title: Development of a database of amino acid sequences for proteins identified and isolated on two-dimensional polyacrylamide gels.
abstract: As part of our continuing studies into the biochemical basis of long-term changes in neuronal function in Aplysia, we have developed a simple method for obtaining amino acid sequence information from proteins isolated on two-dimensional gels. Proteins isolated on preparative two-dimensional gels are digested in situ with Staphylococcus aureus V8 protease, and the resulting peptides electrophoresed, transferred to a polyvinylidene difluoride membrane, and sequenced in a gas-phase sequencer. The method is simple and should be applicable to a variety of other systems where the development of a two-dimensional gel database is underway.
authors.affiliation: Howard Hughes Medical Institute, Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
authors.forename: J D
authors.initials: JD
authors.lastname: Sweatt
authors.forename: T E
authors.initials: TE
authors.lastname: Kennedy
authors.forename: K
authors.initials: K
authors.lastname: Wager-Smith
authors.forename: M A
authors.initials: MA
authors.lastname: Gawinowicz
authors.forename: A
authors.initials: A
authors.lastname: Barzilai
authors.forename: K A
authors.initials: KA
authors.lastname: Karl
authors.forename: E R
authors.initials: ER
authors.lastname: Kandel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia; D002490:Central Nervous System / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D007256:Information Systems; D011506:Proteins / Q000032:analysis*; D012697:Serine Endopeptidases / Q000494:pharmacology
chemical_list: D011506:Proteins; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase