A biochemical study of optic nerve myelin isolated by a micromethod from different aged rats.
A micromethod was used to study myelin from individual rat optic nerves as a function of age. Protein and galactolipid content as well as 2',3'-cyclic nucleotide phosphohydrolase (CNPase) activity at ages 2, 4, 22, and 28 months were determined. Protein content increased significantly at 22 months and decreased at advanced age. Galactolipid content increased significantly at 28 months while CNPase activity decreased. The ratios of protein to galactolipid and CNPase to galactolipid changed with changing age. These data suggest that, unlike the central nervous system, specific biochemical parameters of the optic nerve change with age.
Jacks
A S
AS
Department of Chemistry, Illinois State University, Normal 61761.
Richardson
A R
AR
Jones
M A
MA
D000375:Aging / Q000378:metabolism*; D000818:Animals; D038983:Galactolipids; D006017:Glycolipids / Q000032:analysis; D006801:Humans; D008297:Male; D008832:Microchemistry; D009185:Myelin Proteins / Q000032:analysis; D009186:Myelin Sheath / Q000032:analysis*; D009900:Optic Nerve / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344
D038983:Galactolipids; D006017:Glycolipids; D009185:Myelin Proteins