title: Demonstration of delta rec-pseudo J alpha rearrangement with deletion of the delta locus in a human stem-cell leukemia.
abstract: It has been hypothesized that a rearrangement between the delta recombining element (delta Rec) and a pseudo J alpha gene serves to delete the TCR-delta locus before rearrangement of the TCR-alpha genes. We have now sequenced a direct, site-specific rearrangement between the delta Rec element and a pseudo J alpha gene in a human leukemic stem-cell line. Putative "N-sequence" addition was noted at the site of recombination, suggesting that this event occurred at a time when the enzyme(s) involved in N-region addition were active in this cell. This provides support for the view that deletion of the TCR-delta locus is required before rearrangement of the TCR-alpha chain genes.
authors.affiliation: Navy Medical Oncology, National Cancer Institute, Bethesda, Maryland 20892.
authors.forename: C G
authors.initials: CG
authors.lastname: Begley
authors.forename: P D
authors.initials: PD
authors.lastname: Aplan
authors.forename: M P
authors.initials: MP
authors.lastname: Davey
authors.forename: J P
authors.initials: JP
authors.lastname: de Villartay
authors.forename: D I
authors.initials: DI
authors.lastname: Cohen
authors.forename: T A
authors.initials: TA
authors.lastname: Waldmann
authors.forename: I R
authors.initials: IR
authors.lastname: Kirsch
mesh_terms: D000208:Acute Disease; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002872:Chromosome Deletion; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000235:genetics*; D007938:Leukemia / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
chemical_list: D007133:Immunoglobulin Joining Region; D011948:Receptors, Antigen, T-Cell