Activation of the mouse metallothionein-I promoter in transiently transfected avian cells.
The induction of the mouse metallothionein-I (MT-I) transcriptional regulatory region by heavy metals in cultured avian cells was studied in a transient-expression assay system for a growth hormone. The MT-I promoter was shown to be inducible by 10 microM to 90 microM ZnCl2 in a dose-dependent manner.
Kelder
B
B
Department of Zoological and Biomedical Sciences, Ohio University, Athens 45701.
Chen
H
H
Kopchick
J J
JJ
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002712:Chlorides / Q000494:pharmacology; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics; D008668:Metallothionein / Q000235:genetics*; D010957:Plasmids; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D014162:Transfection; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds
D002712:Chlorides; D017967:Zinc Compounds; C016837:zinc chloride; D013006:Growth Hormone; D008668:Metallothionein; D015032:Zinc