Altered contents of tocopherols in chickens with inherited muscular dystrophy.
Oxidative damage has been hypothesized as the basis for some of the changes in enzymatic functions and physical properties of membranes in inherited muscular dystrophy. The contents of alpha- and gamma-tocopherol (vitamin E) and their oxidation products, the tocopheryl quinones, were measured at 1 to 4 weeks after hatching in the muscle and other tissues of chickens with inherited muscular dystrophy. Analyses at these early ages minimized the potential influence of pathological changes on the measured parameters. The affected muscle (pectoralis major) of dystrophic birds contained significantly higher levels of alpha-tocopheryl quinone and a decreased ratio of alpha- to gamma-tocopherol. Consistent changes in these parameters were not observed in other tissues. Although their basis remains unclear, these changes in the tocopherols are suggestive of oxidative stress in dystrophic muscle membranes. Lipid extracts of tissues of normal and dystrophic birds exhibited no significant differences in the content of conjugated dienes or lipofuscins, two other indices of oxidative stress. These data do not consistently support the hypothesis that oxidative stress plays a causal role in damage to dystrophic muscle, although it remains possible that free-radical damage is involved in the secondary alterations associated with muscular dystrophy.
Murphy
M E
ME
Division of Pharmacology and Toxicology, College of Pharmacy, University of Texas, Austin 78712-1074.
Kehrer
J P
JP
D000375:Aging / Q000378:metabolism; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D008062:Lipofuscin / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism*
D008062:Lipofuscin; D014810:Vitamin E