title: Identification of a procarboxypeptidase A-truncated protease E binary complex in human pancreatic juice.
abstract: The characterization, in human pancreatic juice, of a binary complex associating procarboxypeptidase A with a 32 kDa inactive glycoprotein (G32) is reported in this paper. Free G32 was isolated after dissociation of the binary complex. N-terminal sequence analysis revealed a complete homology between this protein and human protease E (HPE 1), except for the two strongly hydrophobic N-terminal residues (Val-Val) which are missing in G32. This protein might be a truncated protease E highly analogous to the subunit III of the ruminant procarboxypeptidase A-S6 ternary complex. The analogy with bovine subunit III is further supported by interspecies reassociation experiments showing that bovine procarboxypeptidase A can specifically bind human G32.
authors.affiliation: Centre de Biochimie et de Biologie Moléculaire du CNRS, Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Moulard
authors.forename: B
authors.initials: B
authors.lastname: Kerfelec
authors.forename: B
authors.initials: B
authors.lastname: Mallet
authors.forename: C
authors.initials: C
authors.lastname: Chapus
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002268:Carboxypeptidases / Q000378:metabolism*; D043422:Carboxypeptidases A; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D010189:Pancreatic Juice / Q000201:enzymology*; D011487:Protein Conformation; D012697:Serine Endopeptidases / Q000378:metabolism*; D015032:Zinc / Q000032:analysis
chemical_list: D002241:Carbohydrates; D004792:Enzyme Precursors; D006023:Glycoproteins; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D012697:Serine Endopeptidases; C028734:protease E; D015032:Zinc