Effect of phenylarsine oxide on protein synthesis in 3T3-L1 adipocytes.
In this report, we show that insulin stimulated the incorporation of tracer [3H]leucine into protein of 3T3-L1 adipocytes within 2 min of insulin addition. The concentration of insulin required to elicit 50% activation was 4nM. Phenylarsine oxide, an inhibitor of insulin-stimulated glucose transport, blocked not only insulin-stimulated protein synthesis but constitutive protein synthesis as well (Ki, 3 microM). Importantly, protein synthesis was not required for insulin-activated glucose transport since cycloheximide added either before or after insulin had no effect on the stimulated rates of glucose transport.
Pettengell
K
K
Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville 32610.
Frost
S C
SC
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001152:Arsenicals / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007329:Insulin Antagonists; D051379:Mice; D014176:Protein Biosynthesis
D001152:Arsenicals; D007329:Insulin Antagonists; C029341:oxophenylarsine; D003513:Cycloheximide; D005947:Glucose