Rapid identification of medically important yeasts by electrophoretic protein patterns.
A simple electrophoretic method for yeast identification was evaluated. Whole cells were extracted by SDS and the protein profiles obtained in SDS-PAGE after Coomassie blue staining were compared for 52 strains from 9 species of yeast or yeast-like fungi commonly isolated from man (Candida albicans, C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis, C. pseudotropicalis, C. tropicalis, Geotrichum candidum, Saccharomyces cerevisiae). The corresponding patterns showed 30 to 45 polypeptides in the range 95-20 kDa and were clearly different for the 9 species. No differences could be detected between strains from the same species. The characteristic patterns were obtained within 24 h allowing rapid identification of the most commonly encountered clinical yeast isolates.
Bruneau
S
S
Centre d'Immunochimie Microbienne, Institut Pasteur, L'Arbresle, France.
Guinet
R
R
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005656:Fungal Proteins / Q000302:isolation & purification; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D015003:Yeasts / Q000145:classification / Q000302:isolation & purification*
D005656:Fungal Proteins; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; C048139:Coomassie blue