title: Evaluation of Pasteurella multocida mutants of low virulence. I. Development and pathogenicity.
abstract: Mutagenesis of the Clemson University (CU) vaccine strain of Pasteurella multocida with N-methyl-N-nitro-N-nitrosoguanidine resulted in temperature-sensitive mutants that grew at 37 C but not at 42 C. Seven such mutants were evaluated for immunogenicity in turkeys. From these seven, only two, PM#1 and PM#3, provided turkeys with a level of protection against challenge with a virulent serotype 3 P. multocida strain (P-1059) comparable to the protection provided by the CU strain. Intravenous (IV) inoculation of PM#1, PM#3, or CU was used to assess differences in virulence. PM#1 and PM#3 resulted in lower rates of mortality and lameness than the CU strain. Histopathological evaluation of spleens 24, 48, and 72 hours after IV inoculation demonstrated that the CU strain induced significantly more fibrinoid necrosis of the spleen than either PM#1 or PM#3.
authors.affiliation: Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
authors.forename: C L
authors.initials: CL
authors.lastname: Hofacre
authors.forename: J R
authors.initials: JR
authors.lastname: Glisson
authors.forename: S H
authors.initials: SH
authors.lastname: Kleven
authors.forename: J
authors.initials: J
authors.lastname: Brown
authors.forename: G N
authors.initials: GN
authors.lastname: Rowland
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology; D009154:Mutation; D010325:Pasteurella / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D010326:Pasteurella Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology*; D013696:Temperature; D014422:Turkeys / Q000276:immunology*; D014774:Virulence
chemical_list: D001428:Bacterial Vaccines