title: Species-specific responses of muscle lactate dehydrogenase isozymes to increased contractile activity.
abstract: Analyses of lactate dehydrogenase (LDH) isozymes were performed in normal and chronically stimulated (10 Hz, 10 h/d) tibialis anterior (TA) muscles of the mouse, rat, guinea pig, and rabbit. The electrophoretic mobilities of the individual LDH isozymes of normal muscles revealed species-specific patterns. Stimulation up to 28 days evoked increases in the percentage of the H-subunit of LDH. The absolute increase was highest in the rabbit, intermediate in the guinea pig and rat, and lowest in the mouse. The extent of the M- to H-subunit exchange, thus, appeared to be inversely correlated with the basal aerobic-oxidative capacities of the investigated muscles. In addition, a relationship between the expression of the H-subunit of LDH and the increase in aerobic-oxidative capacity became evident from these stimulation-induced processes of metabolic adaptation.
authors.affiliation: Fakultät für Biologie, Universität Konstanz, Federal Republic of Germany.
authors.forename: J A
authors.initials: JA
authors.lastname: Simoneau
authors.forename: D
authors.initials: D
authors.lastname: Pette
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009119:Muscle Contraction; D009132:Muscles / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase