title: An endpoint enzymatic assay for fructose 2,6-bisphosphate performed in 96-well plates.
abstract: An endpoint enzymatic assay for fructose 2,6-bisphosphate based on the ability of the compound to stimulate pyrophosphate 6-phosphofructo-1-kinase and performed in a 96-well plate is reported here. The method presents a low detection limit and a high sensitivity that could be further improved; moreover, the use of 96-well plates greatly increases the number of samples that can be simultaneously assayed.
authors.affiliation: Department of Biochemistry, University of Florence, Firenze, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Bruni
authors.forename: V
authors.initials: V
authors.lastname: Vasta
authors.forename: M
authors.initials: M
authors.lastname: Farnararo
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005635:Fructosediphosphates / Q000032:analysis*; D006598:Hexosediphosphates / Q000032:analysis*; D009243:NAD / Q000032:analysis
chemical_list: D005635:Fructosediphosphates; D006598:Hexosediphosphates; D009243:NAD; C027652:fructose 2,6-diphosphate