[A study on measles immune status by ELISA].
Both ELISA and HI tests have been used to test 85 paired sera collected shortly before and after a measles epidemic. Results showed that 22 children whose pre-exposure antibody titers were less than 1:2 by HI and less than 1:100 by ELISA contracted measles subsequently and other 2 children, one less than 1:2 by HI and 1:100 by ELISA, one 1:2 by HI and less than 1:100 by ELISA contracted too. 3 of 5 children whose pre-exposure antibody titers ranged from 1:2 to 1:8 by HI and 1:100 to 1:400 by ELISA, contracted the disease and 2 of them developed a subclinical infection. The antibody titers of 56 persons with pre-exposure was 1:16 by HI and greater than or equal to 1:400 by ELISA neither contracted measles nor displayed an antibody enhancement. The comparison between the two tests showed that when the antibody titer of ELISA less than 1:200 was regarded as a negative mark, the sensitivity of this test was 100% and specificity was 95.2%. The ELISA can be used in place of HI test for immune status surveillance against measles.
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D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007223:Infant; D008457:Measles / Q000276:immunology* / Q000517:prevention & control; D008459:Measles virus / Q000276:immunology
D000914:Antibodies, Viral