Testing of hydralazine in in vivo-in vitro hepatocyte assays for UDS and stimulation of replicative DNA synthesis.
The vasodilator hydralazine was tested for induction of DNA-repair synthesis and stimulation of replicative DNA synthesis in rat hepatocytes after administration in vivo, either once or repetitively. No increase in unscheduled or replicative DNA synthesis was observed. By contrast, positive controls clearly induced DNA-repair synthesis, either after a single treatment (4-aminobiphenyl, dimethylnitrosamine and methyl methanesulphonate) or after repetitive treatment (benzo[a]pyrene), or stimulated replicative DNA synthesis (carbon tetrachloride and dimethylnitrosamine). Thus, hydralazine displayed no genotoxic and no tumour-promoting activity in these in vivo-in vitro test systems.
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Ciba-Geigy Ltd., Basel, Switzerland.
Müller
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D000818:Animals; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D006830:Hydralazine / Q000633:toxicity*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens; D051381:Rats
D009153:Mutagens; D006830:Hydralazine