title: An efficient laboratory made apparatus for DNA amplification.
abstract: DNA amplification by the polymerase chain reaction (PCR) is a method capable of producing a selective and very high enrichment of a specific DNA sequence. Hence it seems to be useful in various fields from basic research to clinical applications. In order to automatize PCR we assembled for a very low cost a mechanical system designed to carry a test tube holder successively in three thermal baths set at the required temperatures for the reaction. Two examples of the use of this machine are given: (i) amplification of DNA of a particular subtype of acute intermittent porphyria; (ii) the detection of the chimeric c-abl/bcr message found in chronic myelogenous leukemia cells.
authors.affiliation: INSERM U.160, Hôpital Beaujon, Clichy, France.
authors.forename: O
authors.initials: O
authors.lastname: Bertrand
authors.forename: M H
authors.initials: MH
authors.lastname: Delfau
authors.forename: M
authors.initials: M
authors.lastname: Garbarz
authors.forename: C
authors.initials: C
authors.lastname: Picat
authors.forename: I
authors.initials: I
authors.lastname: Devaux
authors.forename: D
authors.initials: D
authors.lastname: Dhermy
authors.forename: P
authors.initials: P
authors.lastname: Boivin
authors.forename: B
authors.initials: B
authors.lastname: Grandchamp
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D011164:Porphyrias / Q000235:genetics; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D004247:DNA; D004259:DNA-Directed DNA Polymerase