Electrically regulated cellular morphological and cytoskeletal changes on an optically transparent electrode.
Electrically regulated morphological and cytoskeletal changes of HeLa cells were studied on an optically transparent electrode (OTE), on which potential-applied surface cellular behavior and morphogenesis were easily observed. Upon application of a potential, HeLa cells in an OTE exhibited remarkable morphological changes above +0.5 V (vs. Ag/AgCl) and below 0 V. At each potential in this potential range, change in F-actin distribution was observed using a fluorescent probe (rhodamine phalloidin). These results suggest that an electrical field induces a subcellular cytoskeletal change. Electrostimulation of cells with OTE can be a valuable strategy for the manipulation of cultured human cells.
Yaoita
M
M
Department of Bioengineering, Tokyo Institute of Technology, Japan.
Aizawa
M
M
Ikariyama
Y
Y
D000199:Actins / Q000378:metabolism*; D002462:Cell Membrane / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D004558:Electric Stimulation; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008839:Microelectrodes
D000199:Actins