title: Synthesis and aromatization of 19-norandrogens in the stallion testis.
abstract: The results of the measurement of 19-nortestosterone in the testiscular artery and vein of the stallion, the very low levels of this steroid in the peripheral blood of geldings and the similar patterns of increase in the peripheral levels of 19-nortestosterone and testosterone after hCG stimulation, show that 19-nortestosterone, like testosterone, is essentially synthesized in the testis. This testicular origin was confirmed by the ability of testicular tissue to synthesize 19-norandrogens from [4-14C]androgens in vitro. 19-Nortestosterone was 50% conjugated in the peripheral blood and almost entirely conjugated after biosynthesis in vitro. The sequence of appearance of steroids in the peripheral blood after a single injection of 10,000 IU hCG suggests that, in the equine testis, 19-norandrogens are produced by a specific C10-19 desmolase (estrene synthetase), stimulable by hCG. 19-Nortestosterone was aromatized into estradiol-17 beta by stallion testicular microsomes. The affinity of the aromatase for 19-nortestosterone was very low compared to that for testosterone. At low and presumably physiological levels, and at a high testosterone/19-nortestosterone ratio, testosterone did not inhibit 19-nortestosterone aromatization by more than 53%. Thus, 19-nortestosterone may be aromatized in vivo in the testis in spite of the endogenous concentrations of androgens. However, the low velocity of 19-nortestosterone aromatization by testicular microsomes at roughly physiological concentrations suggests that 19-norandrogen aromatization may only participate slightly in the testicular estrogen production. These results suggest that in the equine testis, two aromatizing enzyme systems may exist: one which aromatizes both androgens and 19-norandrogens, and a minority system more specific for 19-norandrogens.
authors.affiliation: Laboratoire de Biochimie, UA CNRS 609, Caen, France.
authors.forename: T
authors.initials: T
authors.lastname: Dintinger
authors.forename: J L
authors.initials: JL
authors.lastname: Gaillard
authors.forename: I
authors.initials: I
authors.lastname: Zwain
authors.forename: R
authors.initials: R
authors.lastname: Bouhamidi
authors.forename: P
authors.initials: P
authors.lastname: Silberzahn
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000378:metabolism; D006736:Horses / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009277:Nandrolone / Q000096:biosynthesis / Q000378:metabolism*; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D013739:Testosterone; D004958:Estradiol; D009277:Nandrolone; D001141:Aromatase