Dual effects of pertussis toxin on in vitro invasive behavior of metastatic lymphoma variants.
A simple monolayer invasion assay (MIA) was recently developed using confluent fibroblastic cells as a target and variants of the BW5147 murine T-cell lymphoma as invading cells. Metastatic variants were consistently invasive in the MIA whereas non-metastatic cells were not. In this paper it is reported that pertussis toxin (PT) treatment of a highly metastatic and invasive variant caused a marked delay of invasion in the MIA at concentrations from 50 pg/ml upwards. Surprisingly, PT treatment of the non-metastatic, non-invasive parental BW5147 cells induced a moderate but significant level of invasion. Morphometric analysis showed that PT provoked an increased pseudopodal activity in cells in which it also caused increased invasive potential, and a decreased motility in cells with decreased invasiveness. This finding strengthens the perception that invasive potential and the capability to perform shape changes are related characteristics in these lymphoma cells.
Verschueren
H
H
Pasteur Instituut van Brabant, Brussels, Belgium.
Van Hecke
D
D
Hannecart-Pokorni
E
E
Dekegel
D
D
De Baetselier
P
P
D000818:Animals; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008223:Lymphoma / Q000473:pathology*; D051379:Mice; D008809:Mice, Inbred C3H; D009361:Neoplasm Invasiveness; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin