title: The large viral RNA segment of California serogroup bunyaviruses encodes the large viral protein.
abstract: Reassortant bunyaviruses derived from two members of the California serogroup (La Crosse/original and Tahyna/181-57) viruses were used to demonstrate that the large Mr viral protein (L) is encoded by the L RNA segment. Radiolabelled viral proteins were analysed by discontinuous SDS-PAGE. The L protein of La Crosse virus was observed to migrate ahead of its Tahyna virus counterpart when electrophoresed through a 5% acrylamide resolving gel. Among the reassortant viruses, the L protein phenotype segregated with the viral L RNA segment. After confirming the genotype of the viruses used in this study, it was concluded that the L RNA species of California serogroup viruses codes for the L protein, the presumed viral polymerase.
authors.affiliation: Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
authors.forename: M J
authors.initials: MJ
authors.lastname: Endres
authors.forename: D R
authors.initials: DR
authors.lastname: Jacoby
authors.forename: R S
authors.initials: RS
authors.lastname: Janssen
authors.forename: F
authors.initials: F
authors.lastname: Gonzalez-Scarano
authors.forename: N
authors.initials: N
authors.lastname: Nathanson
mesh_terms: D000818:Animals; D002043:Bunyaviridae / Q000235:genetics*; D002460:Cell Line; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D002141:Encephalitis Virus, California / Q000145:classification / Q000235:genetics*; D005838:Genotype; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D015342:DNA Probes; D012367:RNA, Viral; D014764:Viral Proteins