title: A subset of human rosetted lymphocytes contains previously unidentified histamine.
abstract: By sequential solid-phase immunoadsorption (panning) steps, we have isolated a subset of lymphocytes (comprising 3-7% of rosetted cells) that contains high concentrations of histamine. We have used a radioenzymatic assay for the determination of histamine and have located 117 ng of histamine/1 x 10(6) cells in Leu-5+ (OKT-11), Leu-15+ cells. This subset did not contain basophils and was negative for Leu-4 (OKT-3), Leu-3 (OKT-4), Leu-2 (OKT-8), and 9.3 antigens. The function of this subset of rosetted cells has not been determined.
authors.affiliation: Department of Medicine, Stanford University School of Medicine, California 94305.
authors.forename: M M
authors.initials: MM
authors.lastname: Khan
authors.forename: D M
authors.initials: DM
authors.lastname: Fishwild
authors.forename: K L
authors.initials: KL
authors.lastname: Melmon
authors.forename: S
authors.initials: S
authors.lastname: Coutre
authors.forename: E G
authors.initials: EG
authors.lastname: Engleman
authors.forename: J A
authors.initials: JA
authors.lastname: Bauer
mesh_terms: D000943:Antigens, Differentiation / Q000032:analysis; D006632:Histamine / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000145:classification; D012397:Rosette Formation
chemical_list: D000943:Antigens, Differentiation; D006632:Histamine