Application of capillary isotachophoresis to the analysis of glutathione conjugates.
An analytical capillary isotachophoretic method has been applied for the quantitative assay of reduced glutathione (GSH) conjugates produced by the cytosolic enzyme GSH S-transferase. The donor molecule GSH in reduced and oxidized (GSSG) forms and the GSH conjugates of at least two electrophilic acceptors can be assayed in a single analysis. This technique also permits the quantitative assay of further metabolites of GSH conjugates including mercapturic acids. The total analysis time was of the order of 30 min. The sensitivity of the method is sufficient for the accurate detection of 0.15 nmol GSH conjugate of 1-chloro-2,4-dinitrobenzene and p-nitrobenzyl chloride, 0.2 nmol GSH conjugate of 1,2-epoxy-3-(p-nitrophenoxy)-propane and 0.15 nmol GSH. The present method is simple, accurate and does not require radioactively labelled compounds or separate analytical procedures.
Tsikas
D
D
Division of Gastroenterology and Hepatology, Medizinische Hochschule Hannover, F.R.G.
Brunner
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G
D000818:Animals; D002384:Catalysis; D004586:Electrophoresis / Q000379:methods*; D005978:Glutathione / Q000032:analysis*; D005982:Glutathione Transferase / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D005982:Glutathione Transferase; D005978:Glutathione