Isolation and identification of S-adenosylmethionine from human urine.
Procedures are described for the isolation and identification of adenosylmethionine from human urine. Previously described preliminary separative procedures using anion and cation exchange columns and an XAD-4 resin column have been extended to permit the separation of adenosylmethionine. The adenosylmethionine has been identified by conversion to methylthioadenosine followed by rechromatography of the latter compound with three different types of columns and elution systems. Mean adenosylmethionine values for urine were as follows: adults, 0.26; children, 0.36 nmole/mumole creatinine. Recovery of adenosylmethionine added to urine and determined by this separative procedure was 52%.
Mills
G C
GC
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.
D000328:Adult; D000375:Aging / Q000378:metabolism; D002021:Buffers; D002675:Child, Preschool; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D008297:Male; D012436:S-Adenosylmethionine / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet
D002021:Buffers; D012436:S-Adenosylmethionine