A follow-up on functional parameters of blood stored in ACD at +4 degrees C.
The main functional parameters of blood stored at +4 degrees C in ACD, according to the common transfusional practice, have been carefully followed in the course of 40 days. The expected depletion of DPG takes place within 10 days, but apparently, no increase of the Hb affinity towards oxygen is observed in this period (or later), because pH lowering acts in the opposite direction during the same time. However, the intrinsic increased affinity of Hb is promptly revealed if the "actual" pHs are corrected at the standard value of 7.4, and/or are extrapolated at this pH from Bohr effect.
Scaroina
F
F
Amprimo
M C
MC
Zola
F
F
Cavallari
L
L
Milanesio
L
L
Bonetti
G
G
Rabino Massa
E
E
Ricco
G
G
D019794:2,3-Diphosphoglycerate; D001793:Blood Preservation; D019343:Citric Acid; D003080:Cold Temperature; D004163:Diphosphoglyceric Acids / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis* / Q000187:drug effects; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D013997:Time Factors
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; C002113:acid citrate dextrose; D019343:Citric Acid; D005947:Glucose