Structure-potential dependence of adsorbed enzymes and amino acids revealed by the surface enhanced Raman effect.
Surface enhanced Raman scattering (SERS) of some enzymes (alkaline phosphatase, horseradish peroxidase and lactoperoxidase) and some amino acids (tryptophan, tyrosine and phenylalanine) on silver electrodes has been studied. The spectral band intensities of certain amino acids and amino acid residues were determined by their orientation on the surface and depended on the electrode potential (E).
Vidugiris
G J
GJ
Institute of Biochemistry, Lithuanian Academy of Sciences, Vilnius, USSR.
Gudavicius
A V
AV
Razumas
V J
VJ
Kulys
J J
JJ
D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000596:Amino Acids; D004798:Enzymes / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism; D007784:Lactoperoxidase / Q000378:metabolism; D008968:Molecular Conformation; D011199:Potentiometry; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman / Q000379:methods
D000596:Amino Acids; D004798:Enzymes; D006735:Horseradish Peroxidase; D007784:Lactoperoxidase; D000469:Alkaline Phosphatase