Methylation of 23S rRNA caused by tlrA (ermSF), a tylosin resistance determinant from Streptomyces fradiae.
Ribosomes from Streptomyces griseofuscus expressing tlrA, a resistance gene isolated from the tylosin producer Streptomyces fradiae, are resistant to macrolide and lincosamide antibiotics in vitro. The tlrA product was found to be a methylase that introduces two methyl groups into a single base within 23S rRNA, generating N6,N6-dimethyladenine at position 2058. This activity is therefore similar to the ermE resistance mechanism in Saccharopolyspora erythraea (formerly Streptomyces erythraeus).
Zalacain
M
M
Department of Biochemistry, University of Leicester, United Kingdom.
Cundliffe
E
E
D004352:Drug Resistance, Microbial / Q000235:genetics; D007700:Kinetics; D007933:Leucomycins / Q000494:pharmacology*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D010957:Plasmids; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D014158:Transcription, Genetic; D015645:Tylosin
D007933:Leucomycins; D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase; D015645:Tylosin