The diagnosis of human hydatidosis by measurement of specific IgE antibody by enzyme immunoassay.
The sensitivity and specificity of an enzyme immunosorbent assay (EIA) for measurement of anti-Echinococcus granulosus specific IgE antibody using microtiter strips was evaluated. The sensitivity was 92.2% for 90 patients with cysts in the liver or other body sites but excluding the patients with cysts in the lungs or bones. In this latter group (n = 26) a sensitivity of 61.5% was recorded. The specificity for control groups comprising 89 blood bank sera from Spain and 48 sera from atopic Swedes was 100%. Patients infected with other parasites (n = 78) were usually negative (81%). 11 of the 15 false positives were found in patients with total IgE greater than 5,000 kU/l. The microtiter EIA method employed can be considered as a very convenient method to be included in the range of immunodiagnostic tests for human hydatid disease.
Sjölander
A
A
Pharmacia Diagnostics AB, Uppsala, Sweden.
Guisantes
J A
JA
Torres-Rodriguez
J M
JM
Schröder
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000918:Antibody Specificity; D002648:Child; D004443:Echinococcosis / Q000175:diagnosis* / Q000276:immunology; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests
D000909:Antibodies, Helminth; D007073:Immunoglobulin E