The large viral RNA segment of California serogroup bunyaviruses encodes the large viral protein.
Reassortant bunyaviruses derived from two members of the California serogroup (La Crosse/original and Tahyna/181-57) viruses were used to demonstrate that the large Mr viral protein (L) is encoded by the L RNA segment. Radiolabelled viral proteins were analysed by discontinuous SDS-PAGE. The L protein of La Crosse virus was observed to migrate ahead of its Tahyna virus counterpart when electrophoresed through a 5% acrylamide resolving gel. Among the reassortant viruses, the L protein phenotype segregated with the viral L RNA segment. After confirming the genotype of the viruses used in this study, it was concluded that the L RNA species of California serogroup viruses codes for the L protein, the presumed viral polymerase.
Endres
M J
MJ
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Jacoby
D R
DR
Janssen
R S
RS
Gonzalez-Scarano
F
F
Nathanson
N
N
D000818:Animals; D002043:Bunyaviridae / Q000235:genetics*; D002460:Cell Line; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D002141:Encephalitis Virus, California / Q000145:classification / Q000235:genetics*; D005838:Genotype; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D015342:DNA Probes; D012367:RNA, Viral; D014764:Viral Proteins