Correlation of pause sites in MDV-1 RNA replication with kinetic refolding of the growing chain. A Monte Carlo simulation of the Markov process.
We consider a template-instructed MDV-1 RNA replication catalyzed by Q beta replicase. The relaxation of the effective binding of the growing chain to the enzyme is simulated, considering a Markov process where refolding events in the chain are concomitant with chain elongation events. When realistic transition probabilities are considered, it becomes evident that the pause sites in replication are due to relaxation of the binding by folding of the growing chain.
Fernández
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Max-Planck-Institut für Biophysikalische Chemie, Göttingen.
D002384:Catalysis; D007700:Kinetics; D008390:Markov Chains; D009010:Monte Carlo Method; D009690:Nucleic Acid Conformation; D009874:Operations Research; D011336:Probability; D011777:Q beta Replicase; D012316:RNA Nucleotidyltransferases; D013698:Templates, Genetic
D011777:Q beta Replicase; D012316:RNA Nucleotidyltransferases