[Diminution of the primary antibody response in rats without seminal vesicles].
A group of 180 male Wistar rats was utilized to study the synthesis of antibodies against sheep red blood cells (SRBC) after the surgical extirpation of seminal vesicles at 8-10 days of age. Rats were randomly divided into three groups of 60 animals. Group 1 had the rats which seminal vesicles were removed surgically. The second group had the sham-operated controls which glands were exposed only and the incision was closed forthwith. Animals of group 3 were normal and nonoperated. Between 1-6 months of age the rats were immunized intraperitoneally with a SRBC suspension. Four days later the animals were sacrificed, the spleens were removed from the abdominal cavity and the lymphocyte suspensions were obtained after homogenization of the tissues. Adjusted splenic viable lymphocyte suspensions were mixed with SRBC and guinea pig serum to count the anti-erythrocyte hemolytic plaque forming cells, according to the modification of Cunningham of the previously described cell lytic assay of Jerne. The amounts of splenic lymphocytes synthesizing antibodies against SRBC in the rats without seminal vesicles were significantly lower (p less than 0.001) than that of the sham-operated animals, except at 30 days of age. Proportions of rats with a number of plaque forming cells (PFC) above the median value were significantly lower (p less than 0.001) in the group of rats without seminal vesicles. A X test was used to explore differences between the proportions observed in the three groups.(ABSTRACT TRUNCATED AT 250 WORDS)
García-Tamayo
F
F
Ocampo-Lujano
A
A
Hernández
R
R
D000367:Age Factors; D000818:Animals; D000917:Antibody Formation; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012669:Seminal Vesicles / Q000502:physiology*
Disminución de la respuesta primaria de anticuerpos en ratas sin vesículas seminales.