Prolactin stimulation of protein kinase C activity in rat aortic smooth muscle.
Prolactin (PRL) activated protein kinase C (PKC) in a dose dependent manner in rat aortic smooth muscle. Aortic strips incubated with sub-nanomolar concentrations of ovine PRL for 25 min. at 37 degrees C showed a significant stimulation of PKC activity in both cytosolic and particulate fractions. This activation could be blocked using either anti-PRL antibodies or 1-(5- isoquinolinesulfonyl)-2-methylpiperazine (H-7), a PKC inhibitor. The results further support the role of PKC in the signal transduction pathway for PRL action and suggest that this activation may be involved in vascular smooth muscle function.
Sauro
M D
MD
Department of Pharmacology and Therapeutics University of South Florida, College of Medicine, Tampa 33612.
Buckley
A R
AR
Russell
D H
DH
Fitzpatrick
D F
DF
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D001011:Aorta / Q000166:cytology; D003600:Cytosol / Q000032:analysis; D003902:Detergents; D004305:Dose-Response Relationship, Drug; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010879:Piperazines / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology*; D011493:Protein Kinase C / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D003902:Detergents; D007546:Isoquinolines; D010879:Piperazines; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011388:Prolactin; D011493:Protein Kinase C