Identification of glucagon-like peptide-1(7-36) amide in rat brain.
Acid-urea extract of rat brain was examined by glucagon-like peptide-1 (GLP-1) specific radioimmunoassay. A single peak was observed which co-eluted with GLP-1(7-36)amide on gel filtration and anion exchange chromatography. In contrast, GLP-1(1-37) was not detected under our experimental conditions. The fact that GLP-1 (7-36)amide, but not GLP-1(1-37), was present in rat brain suggests that preproglucagon was processed in the brain in the same manner as in the intestine and not as in the pancreas.
Yoshimoto
S
S
Department of Laboratory Medicine, School of Medicine, University of Tokushima, Japan.
Hirota
M
M
Ohboshi
C
C
Shima
K
K
D000818:Animals; D001923:Brain Chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005934:Glucagon; D052216:Glucagon-Like Peptide 1; D004763:Glucagon-Like Peptides; D008297:Male; D010446:Peptide Fragments; D010455:Peptides / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D010446:Peptide Fragments; D010455:Peptides; C055453:glucagon-like peptide 1 (7-36)amide; D004763:Glucagon-Like Peptides; D052216:Glucagon-Like Peptide 1; D005934:Glucagon