title: Role of T3 in thermogenic and trophic responses of brown adipose tissue to cold.
abstract: Cold-induced growth of brown adipose tissue (BAT) was studied in thyroidectomized rats that received low doses of either thyroxine (T4) or 3,5,3'-triidothyronine (T3). The objective was to find out whether the cold-induced increase in activity of T4 5'-deiodinase, and thus increased endogenous T3 generation in BAT itself, was necessary for growth of BAT or whether T3 from the blood could serve as effectively as T3 produced endogenously. The acute thermogenic response of BAT to cold (15 h at 4 degrees C), as measured by the increase in mitochondrial GDP binding, was abolished by thyroidectomy, as seen previously, and restored by T3 as well as by T4 treatment. The long-term trophic response to cold (20-25 days at 4 degrees C), as indicated by increases in protein and DNA and in mitochondrial concentrations of GDP-binding sites and uncoupling protein, occurred whether T3 or T4 was administered to these thyroidectomized rats. We conclude that endogenous T3 production in BAT does not direct and is not essential for the long-term trophic response of this tissue to cold. We are not able to exclude, on the basis of the present results, that an optimal growth rate during the initial phase of the trophic response may require enhanced endogenous production of T3 in BAT. The cold-induced increase in T4 5'-deiodinase activity, presumably mediated by an action of norepinephrine, does not require the presence of either T3 or T4, as seen previously by others.
authors.affiliation: Department of Biochemistry, University of Ottawa, Ontario, Canada.
authors.forename: I R
authors.initials: IR
authors.lastname: Park
authors.forename: D B
authors.initials: DB
authors.lastname: Mount
authors.forename: J
authors.initials: J
authors.lastname: Himms-Hagen
mesh_terms: D000222:Adaptation, Physiological; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D001833:Body Temperature Regulation; D003080:Cold Temperature; D004247:DNA / Q000096:biosynthesis; D006153:Guanosine Diphosphate / Q000378:metabolism; D007453:Iodide Peroxidase / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D013965:Thyroidectomy; D013974:Thyroxine / Q000494:pharmacology; D014284:Triiodothyronine / Q000494:pharmacology / Q000502:physiology*
chemical_list: D014284:Triiodothyronine; D006153:Guanosine Diphosphate; D004247:DNA; D007453:Iodide Peroxidase; D013974:Thyroxine