Fundamental study on latex reagents for agglutination tests.
Competitive adsorption of Fab and Fc fragments on to particles revealed that the main driving forces for the adsorption of Fab and Fc fragments are ionic and hydrophobic forces, respectively. Latex particles were sensitized with antihuman C-reactive protein-antibody under a condition where ionic binding force was suppressed, and hence antibody was supposed to attach to the particles predominantly at the Fc site. The resulting latex indicated a high efficiency for the determination of C-reactive protein. Among the latexes used, a partially hydrolysed styrene-acrylamide copolymer latex was the best with respect to test efficiency and storage stability.
Kawaguchi
H
H
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Sakamoto
K
K
Ohtsuka
Y
Y
Ohtake
T
T
Sekiguchi
H
H
Iri
H
H
D000327:Adsorption; D002097:C-Reactive Protein / Q000276:immunology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007840:Latex; D007841:Latex Fixation Tests
D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007202:Indicators and Reagents; D007840:Latex; D002097:C-Reactive Protein