title: Dry eye before and after therapy with hydroxypropyl methylcellulose. Ultrastructural and cytochemical study in 20 patients.
abstract: We have utilized hydroxypropyl methylcellulose in the therapy of 20 patients suffering from dry eye in the initial stage. The effects of the substance have been evaluated both from a clinical and a morphological standpoint. In particular, transmission and scanning electron microscopy were applied to investigate the ultrastructural features of the conjunctival epithelium before and after treatment. In addition, lectin cytochemistry was carried out to analyse semiquantitatively the presence of glycoconjugates in the conjunctival mucus produced by goblet and non-goblet epithelial cells. Hydroxypropyl methylcellulose did not improve the tear film stability as demonstrated by the clinical tests. The normal surface of the conjunctival epithelium did not appear recovered as well. Only a minimal increase in the density of the second mucus system vesicles was observed. From a cytochemical point of view, the content of glycosidic residues appeared altered in dry-eye patients both before and after treatment. This alteration can lead to changes in the physical and functional properties of the mucus, impairing its role as a surfactant. We conclude that the common treatment by means of lubrificant-like substances palliates the dryness condition. The search for a drug which reintegrates the correct composition of the conjunctival mucus should be seriously considered as a valid alternative.
authors.affiliation: Institute of Ophthalmology, University of Bologna, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Versura
authors.forename: M C
authors.initials: MC
authors.lastname: Maltarello
authors.forename: F
authors.initials: F
authors.lastname: Stecher
authors.forename: R
authors.initials: R
authors.lastname: Caramazza
authors.forename: R
authors.initials: R
authors.lastname: Laschi
mesh_terms: D001706:Biopsy; D003228:Conjunctiva / Q000187:drug effects / Q000648:ultrastructure*; D015352:Dry Eye Syndromes / Q000628:therapy*; D004847:Epithelial Cells; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D065347:Hypromellose Derivatives; D037102:Lectins / Q000378:metabolism; D008297:Male; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000627:therapeutic use; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D013666:Tears / Q000378:metabolism
chemical_list: D037102:Lectins; D065347:Hypromellose Derivatives; D008747:Methylcellulose