Exposure level monitor of 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole, a dietary carcinogen, in rabbits.
The present investigation describes a method for the detection of minute amounts of 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a carcinogenic tryptophan pyrolysate, bound to the hemoglobin of erythrocytes and plasma from rabbits dosed orally with the dietary carcinogen. The method consists of the acid-induced release of the dietary carcinogen adducts as the free carcinogen and their extraction with methylene chloride and subsequent quantitation by high-performance liquid chromatography (HPLC). With this method, Trp-P-1 levels in plasma, platelets, and red blood cells were monitored for 12 weeks to determine a suitable indicator for estimating the exposure levels of the dietary carcinogen. The results suggest that Trp-P-1 in red blood cells that bound covalently to the hemoglobin is the most suitable for monitoring the long-term exposure levels.
Manabe
S
S
Department of Hygiene & Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Kanai
Y
Y
Wada
O
O
D000284:Administration, Oral; D000818:Animals; D001792:Blood Platelets / Q000032:analysis; D002243:Carbolines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D019286:Endopeptidase K; D004912:Erythrocytes / Q000032:analysis; D008297:Male; D009153:Mutagens / Q000032:analysis*; D011817:Rabbits; D012697:Serine Endopeptidases / Q000494:pharmacology
D002243:Carbolines; D009153:Mutagens; D012697:Serine Endopeptidases; D019286:Endopeptidase K; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole