Effect of phenobarbital on the expression of glutathione S-transferase isoenzymes in cultured rat hepatocytes.
Cultured adult rat hepatocytes were treated daily with 3.2 mM phenobarbital (PB) in order to study its effect on the expression of cytosolic glutathione S-transferase isoenzymes. Glutathione S-transferase (GST) activities, using 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates, were increased when PB was present in the culture medium. After purification and separation of GST on glutathione Sepharose 6 B and reversed-phase HPLC, respectively, it was observed in vitro that PB caused an increase in the relative amounts of subunits 1, 3 and 7 compared to subunits 2 and 4. Using Northern blot technique, elevated levels of GST subunit 1/2 and 7 mRNA were measured, after addition of PB to the cultures.
Vandenberghe
Y
Y
Dienst Toxicologie, Vrije Universiteit Brussel, Belgium.
Morel
F
F
Foriers
A
A
Ketterer
B
B
Vercruysse
A
A
Guillouzo
A
A
Rogiers
V
V
D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004137:Dinitrochlorobenzene / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D009578:Nitrobenzenes / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004137:Dinitrochlorobenzene; D007527:Isoenzymes; D009578:Nitrobenzenes; D012333:RNA, Messenger; C028328:1,2-dichloro-4-nitrobenzene; D005982:Glutathione Transferase; D010634:Phenobarbital