Determination of antileukemic diimidazolinyl compounds in plasma of experimental animals by high-performance liquid chromatography.
A method is reported for the determination of the fluorescent, antileukemic diimidazolinyl compounds 261/96(1). 253/152(2) and 272/131(3) in plasma. HPLC is performed on a RP-2 10 microns column with a mobile phase of methanol/water (1:1, v/v), to which 0.005 mol/l octanesulfonic acid sodium and 0.003 mol/l dimethyloctylamine are added. Samples are prepared by precipitation of plasma proteins with a mixture of methanol-70% perchloric acid. The assay is linear up to 750 ng/ml for all compounds with limits of determination of 4 ng/ml, 2 ng/ml and 0.5 ng/ml for compounds 1, 2 and 3, respectively. Coefficients of variation are below 10 percent at all concentrations studied.
Gluth
W P
WP
Institut für Pharmazie und Lebensmittelchemie der Universität Erlangen-Nürnberg, BRD.
Dann
O
O
D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics; D001572:Benzofurans / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007093:Imidazoles / Q000097:blood*; D007211:Indoles / Q000097:blood*; D014753:Vinyl Compounds / Q000097:blood
D000970:Antineoplastic Agents; D001572:Benzofurans; D007093:Imidazoles; D007211:Indoles; D014753:Vinyl Compounds