title: A developmental study of the abnormal expression of alpha-cardiac and alpha-skeletal actins in the striated muscle of a mutant mouse.
abstract: BALB/c mice possess a 5' duplication of the alpha-cardiac actin gene which is associated with abnormal levels of alpha-cardiac and alpha-skeletal actin mRNAs in adult cardiac tissue. This mutation therefore provides a potential tool for the study of the inter-relationship between the striated muscle actins. We have examined the expression of this actin gene pair throughout the development of skeletal and cardiac muscle in BALB/c mice. During embryonic and fetal development, the expression of these two genes is indistinguishable from that in normal mice, as determined by in situ hybridization. A quantitative postnatal study demonstrates that in the hearts of normal mice the level of alpha-cardiac actin mRNA declines, whereas that of alpha-skeletal actin increases. In mutant mice, these trends are exaggerated so that whereas normal mice have 95.8% alpha-cardiac mRNA and 4.2% alpha-skeletal mRNA in the adult heart, BALB/c mice have 52.4 and 47.6% of these mRNAs, respectively. This difference is also reflected at the protein level. In developing skeletal muscle, the expression of these genes follows kinetics similar to that observed in the heart with a decrease in the relative level of alpha-cardiac mRNA as the muscle matures. Cardiac actin mRNA levels are again lower in the mutant mouse, but here the effect is less striking because skeletal actin is the predominant isoform. These results are discussed in the context of the interaction between this actin gene pair in developing and adult striated muscle.
authors.affiliation: Delta Biotechnology Ltd., Castle Court, Nottingham, Great Britain.
authors.forename: I
authors.initials: I
authors.lastname: Garner
authors.forename: D
authors.initials: D
authors.lastname: Sassoon
authors.forename: J
authors.initials: J
authors.lastname: Vandekerckhove
authors.forename: S
authors.initials: S
authors.lastname: Alonso
authors.forename: M E
authors.initials: ME
authors.lastname: Buckingham
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D015342:DNA Probes; D005786:Gene Expression Regulation; D006321:Heart / Q000196:embryology / Q000254:growth & development; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008817:Mice, Mutant Strains; D024510:Muscle Development; D009132:Muscles / Q000196:embryology / Q000378:metabolism*; D009154:Mutation; D009206:Myocardium / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D012518:Sarcomeres / Q000378:metabolism
chemical_list: D000199:Actins; D015342:DNA Probes; D015347:RNA Probes; D012333:RNA, Messenger