title: Effect of erythorbic acid administration on activities of drug metabolic enzyme and phosphatases in guinea pigs administered an adequate amount of ascorbic acid.
abstract: The effect of erythorbic acid (ErA) administration on activities of liver aniline hydroxylase, liver acid phosphatase, and serum alkaline phosphatase, and the content of liver cytochrome P-450 was studied to determine whether or not ErA would affect the availability of ascorbic acid (AsA) in normal and AsA-deficient guinea pigs. In experiment I, changes of the enzyme activities and liver cytochrome P-450 content in the guinea pigs administered AsA and/or ErA and sacrificed on days 4, 10, 16, and 30 were examined. Moreover, in experiment II, after 16 days of depletion of AsA, the guinea pigs were administered AsA and/or ErA. These animals were sacrificed on days 0, 4, and 20 of the repletion period, after which the activities of drug metabolic enzyme and phosphatases and content of cytochrome P-450 during recovery were observed. The enzyme activities and cytochrome P-450 content of AsA-supplemented guinea pigs were similar to those of ErA-supplemented animals and also similar to those of both AsA and ErA-supplemented guinea pigs throughout the experimental period. During the repletion of the AsA-depleted guinea pigs, there were no significant differences in these enzyme activities and cytochrome P-450 content among the animals administered AsA and/or ErA. These results suggested that ErA administration may not affect the AsA availability in the guinea pigs.
authors.affiliation: Department of Food and Nutrition, Ochanomizu University, Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Kurata
authors.forename: N
authors.initials: N
authors.lastname: Arakawa
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000494:pharmacology*; D001206:Ascorbic Acid Deficiency / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology*; D008297:Male
chemical_list: C005056:isoascorbic acid; D003577:Cytochrome P-450 Enzyme System; D000815:Aniline Hydroxylase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D001205:Ascorbic Acid