Altered calcium homeostasis in carbon tetrachloride exposed rat hepatocytes.
In situ perfusion of rat liver with carbon tetrachloride (10-15 mM at a final concentration of lul/ml of mineral oil) resulted in a significant (p less than 0.01) loss of microsomal Ca2+ over 5-15 min of exposure. Mitochondrial Ca2+ content increased sharply (2 fold) with concomitant increase in the cytosolic free Ca2+. The changes were associated with a significant (p less than 0.01) decrease in plasma membrane Ca2+ - ATPase activity, reflected by impaired Ca2+ efflux and Ca2+ exchange properties. Our data indicates that impairment of plasma membrane Ca2+ flux contributes substantially to the overall disruption of calcium homeostasis induced by carbon tetrachloride.
Andrabi
K
K
Department of Experimental Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh.
Kaul
N
N
Ganguly
N K
NK
Dilawari
J B
JB
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D006706:Homeostasis; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002251:Carbon Tetrachloride; D002118:Calcium