title: Isolation of a novel prolactin-like cDNA clone from bovine placenta: occurrence of new family members.
abstract: A novel cDNA clone that hybridized to bovine prolactin cDNA was isolated from a bovine (Bos taurus) placental cDNA library and the nucleotide sequence was analyzed. The cDNA clone, named bPLP-III, contained one open reading frame encoding a protein consisting of 239 amino acids. The amino-acid sequence of bPLP-III is 43 and 57% homologous to bovine preprolactin and a bovine prolactin-related protein, bPRC-I, respectively, but only 23% homologous to bovine pregrowth hormone. The predicted mature protein of bPLP-III is distinct from bovine placental lactogens in amino-acid composition, suggesting that bPLP-III is the clone for a new member of prolactin-related mRNA expressed in bovine placenta.
authors.affiliation: Department of Biochemistry, Mie University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Yamakawa
authors.forename: M
authors.initials: M
authors.lastname: Watahiki
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010920:Placenta / Q000032:analysis*; D011257:Pregnancy Proteins / Q000235:genetics; D011388:Prolactin / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011257:Pregnancy Proteins; D011388:Prolactin; D004247:DNA