Fluorometric assay for the measurement of viral neuraminidase in influenza vaccines.
Influenza viruses have two surface glycoproteins: haemagglutinin and neuraminidase which are capable of inducing a significant antibody response following vaccination. All neuraminidases from different strains of influenza A and B viruses are able to hydrolyse alpha-ketosidic linkages between N-acetylneuraminic (sialic) acid and other carbohydrates. In this report, the neuraminidase activity was assayed in various influenza vaccines by using a fluorogenic substrate: the sodium salt of 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. This method was reliable (variation less than 8%) and more sensitive (100 to 1000 times) in less time (incubation time = 15 min) than the Warren assay. Therefore, the method is suitable for the control of the sialidase activity during the processing of influenza vaccines.
Lambré
C R
CR
INSERM U 139, Immunopathologie pulmonaire Hôpital H. Mondor, Créteil, France.
Chauvaux
S
S
Pilatte
Y
Y
D005470:Fluorometry; D006801:Humans; D007252:Influenza Vaccines / Q000032:analysis*; D009439:Neuraminidase / Q000032:analysis*; D009975:Orthomyxoviridae / Q000201:enzymology*
D007252:Influenza Vaccines; D009439:Neuraminidase