Chemical composition of Eubacterium nodatum cell wall peptidoglycan.
The structure of Eubacterium nodatum cell wall peptidoglycan was investigated. The peptide subunit of E. nodatum peptidoglycan has the following structure: L-Ala-D-Glu (Gly)-L-Orn-D-Ala. The carboxyl group of alanine occupying position 4 is attached to the delta-amino group of ornithine of an other subunit by the cross-linking bridge L-Ala-L-Ala-L-Orn. All glycine molecules are connected with the alpha-carboxyl group of glutamic acid with the ratio being 0.5-1. The hydrolysis of E. nodatum peptidoglycan by the S. albus G enzyme proceeds primarily due to the activity of alanyl-alanine endopeptidase, ornithyl-ornithine endopeptidase, ornithyl-alanine endopeptidase, N-acetyl-muramyl-alanine amidase, N-acetylmuramidase and N-acetylglucosaminidase.
Severin
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Department of Microbiology, Okayama University Dental School, Japan.
Kokeguchi
S
S
Kato
K
K
D000596:Amino Acids / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D002850:Chromatography, Gel; D004590:Electrophoresis, Paper; D005051:Eubacterium / Q000032:analysis* / Q000648:ultrastructure; D010457:Peptidoglycan / Q000032:analysis*; D013302:Streptomyces / Q000201:enzymology
D000596:Amino Acids; D010457:Peptidoglycan