Use of the water-soluble fluor sodium salicylate for fluorographic detection of tritium in thin-layer chromatograms and nitrocellulose blots.
We have determined that sodium salicylate, a water-soluble fluor which we use routinely for fluorography with polyacrylamide gels, is also useful for fluorography with thin-layer media. Detection of 3H-labeled material applied to thin-layer chromatography plates, or nitrocellulose membranes, can be enhanced up to 150-fold after treatment with an aqueous solution of 2 M sodium salicylate, while detection of 35S-labeled material is enhanced only about 2-fold. We demonstrate the utility of sodium salicylate fluorography in detecting 3H-labeled palmitic acid following thin-layer chromatography and 3H-labeled proteins following blotting to nitrocellulose.
Lucher
L A
LA
Department of Biological Sciences, Illinois State University, Normal 61761.
Lego
T
T
D001345:Autoradiography; D002855:Chromatography, Thin Layer; D005453:Fluorescence; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D012980:Sodium Salicylate; D014316:Tritium / Q000032:analysis*
D007202:Indicators and Reagents; D008567:Membranes, Artificial; D014316:Tritium; D012980:Sodium Salicylate