title: Inhibitory effect of diethyl pyrocarbonate on the H+/organic cation antiport system in rat renal brush-border membranes.
abstract: We examined the effect of diethyl pyrocarbonate (DEPC), a histidine-specific reagent, on the H+/organic cation antiport system in brush-border membrane vesicles isolated from the rat renal cortex. Pretreatment of membrane vesicles with DEPC resulted in the inhibition of tetraethylammonium transport. This inhibition was reversed by subsequent treatment with hydroxylamine, but not with dithiotreitol. In contrast, the uptake of p-aminohippurate, a typical organic anion, was not inhibited by DEPC pretreatment. In the absence of an H+ gradient, pretreatment with DEPC inhibited the uptake of tetraethylammonium at pH 6.0-7.0, but not at pH 7.5. The Vmax value of tetraethylammonium uptake at pH 7.0 was decreased without any change in the Km value, but the kinetic parameters at pH 7.5 were unchanged. Unlabeled tetraethylamonium did not protect against the inhibition by DEPC. These results suggest that histidine residues in the organic cation carrier are essential for transport at acidic and neutral pH values, but not at alkaline pH values, and that histidine residues play an important role as regulatory sites in the H+/organic cation antiport system rather than as binding sites for organic cations.
authors.affiliation: Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Hori
authors.forename: H
authors.initials: H
authors.lastname: Maegawa
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Katsura
authors.forename: K
authors.initials: K
authors.lastname: Inui
mesh_terms: D000818:Animals; D001692:Biological Transport; D002412:Cations; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D005561:Formates / Q000494:pharmacology*; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000378:metabolism; D010130:p-Aminohippuric Acid / Q000378:metabolism
chemical_list: D002412:Cations; D005561:Formates; D006898:Hydroxylamines; D007093:Imidazoles; D013439:Sulfhydryl Reagents; D013757:Tetraethylammonium Compounds; D019811:Hydroxylamine; D019789:Tetraethylammonium; D006859:Hydrogen; D004047:Diethyl Pyrocarbonate; D004229:Dithiothreitol; D010130:p-Aminohippuric Acid; C012160:N-acetylimidazole