title: Analysis of porcine erythrocyte lysate proteins by horizontal two-dimensional electrophoresis with immobilized pH gradient in the first dimension.
abstract: Horizontal two-dimensional gel electrophoresis with immobilized pH gradients in the first dimension resulted in excellent patterns of porcine erythrocyte lysate proteins. The overwhelming excess of hemoglobin was removed by chloroform-ethanol extraction and the number of spots further increased by acetone precipitation, revealing considerably more protein spots than detected by analyzing the untreated sample. A total of 430 protein spots have been reproducibly obtained for further analysis of genetic variations. A number of enzyme systems have been identified by visual inspection and comparison with previous results. The existence of further multiple forms for other enzymes is also apparent.
authors.affiliation: Lehrstuhl für Tierzucht, Technische Universität München, Freising--Weihenstephan, Federal Republic of Germany.
authors.forename: I
authors.initials: I
authors.lastname: Russ
authors.forename: S
authors.initials: S
authors.lastname: Günther
authors.forename: F
authors.initials: F
authors.lastname: Pirchner
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004912:Erythrocytes / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013552:Swine / Q000097:blood
chemical_list: D001798:Blood Proteins