Protein precoating of polylactide microspheres containing a lipophilic immunopotentiator for enhancement of macrophage phagocytosis and activation.
Biodegradable microspheres containing a lipophilic muramyl dipeptide, MDP-B30, were prepared from a L-lactic acid-glycolic acid copolymer. The effect of precoating the microspheres with water-soluble polymers including proteins on the antitumor activity of mouse peritoneal macrophages (M phi) was investigated. Macrophages activated by phagocytosis of the microspheres exhibited growth inhibitory activity toward Meth-A tumor cells. The activity correlated with the extent of M phi phagocytosis of the microspheres. M phi phagocytosis was greatly augmented by gelatin precoating of the microspheres, resulting in a significant increase of in vitro antitumor activity of M phi by the microspheres. However, potentiation of M phi activity by gelatin precoating was minimal after intraperitoneal injection of the microspheres, but cross-linking of the coated gelatin with glutaraldehyde afforded potentiation of the antitumor activity in vivo.
Tabata
Y
Y
Research Center for Medical Polymers and Biomaterials, Kyoto University, Japan.
Ikada
Y
Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D003470:Culture Media; D004337:Drug Carriers; D005780:Gelatin; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010587:Phagocytosis / Q000187:drug effects*; D011091:Polyesters / Q000494:pharmacology*; D013268:Stimulation, Chemical
D000276:Adjuvants, Immunologic; D003470:Culture Media; D004337:Drug Carriers; D011091:Polyesters; C033616:poly(lactide); D005780:Gelatin