title: Decreased expression of the insulin-responsive glucose transporter in diabetes and fasting.
abstract: Cellular resistance to insulin caused by a reduction in insulin-mediated glucose uptake can be produced in rats by chemically inducing diabetes with streptozotocin and by fasting. Two glucose transporter isoforms are expressed in fat cells: (1) the insulin-responsive species which is found only in fat and muscle, and (2) a species corresponding to the erythrocyte/Hep G2/rat brain transporter. We show here that fat cells isolated from streptozotocin diabetic rats and from fasted rats show a significant (60-80%) decrease in the amount of immunologically detectable insulin-sensitive glucose transporter and no change in the level of the Hep G2/rat brain transporter. Administration of insulin and refeeding, respectively, result in a return of the insulin-sensitive glucose transporter to levels that are normal or slightly above normal. Thus, peripheral tissue insulin resistance could be due to the specific reduction in the amount of insulin-sensitive glucose transporter.
authors.affiliation: Department of Biochemical Endocrinology, Merck Institute for Therapeutic Research, Rahway, New Jersey 07065.
authors.forename: J
authors.initials: J
authors.lastname: Berger
authors.forename: C
authors.initials: C
authors.lastname: Biswas
authors.forename: P P
authors.initials: PP
authors.lastname: Vicario
authors.forename: H V
authors.initials: HV
authors.lastname: Strout
authors.forename: R
authors.initials: R
authors.lastname: Saperstein
authors.forename: P F
authors.initials: PF
authors.lastname: Pilch
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005215:Fasting; D015151:Immunoblotting; D007333:Insulin Resistance; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009004:Monosaccharide Transport Proteins