Species specific variant of human centromeric DNA repeats: localization on chromosome 18 and recent amplification in human ancestral line.
Plasmid library of rapidly renaturating fraction of human DNA was constructed. The library was used for isolation of primate-specific DNA repeats. A clone (1hsp-4) which was intensively hybridizable with human DNA exclusively and produced no signals when hybridized with animal DNAs including the ones from orangoutan and chimpanzee was isolated. The cloned sequences 1hsp-4 have been found to be highly specific to centromeric heterochromatin of the 18 chromosome. Primary structure of a short 1hsp-4 fragment has been determined. The obtained data suggest the emergence of a DNA family homologous to the 1hsp-4 probe to be due to the thousandfold leapwise amplification occuring less than 5-8 millions years ago.
Rogaev
E I
EI
Vetchinkina
A A
AA
Iurov
Iu B
IuB
D000818:Animals; D005075:Biological Evolution; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D004247:DNA / Q000235:genetics*; D005784:Gene Amplification; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA
Vidospetsificheskiĭ variant tsentromernykh povtorov DNK cheloveka: lokalizatsiia na 18-ĭ khromosome i nedavniaia amplifikatsiia v predkovoĭ linii cheloveka.