Anti-gliadin antibody specificity for gluten-derived peptides toxic to coeliac patients.
The specificities of serum and intestinal antibodies from coeliac and normal individuals towards gluten-derived peptides, known to be toxic in coeliac disease, has been investigated. Though untreated coeliacs had high serum antibody levels towards gliadin and some gluten-derived peptides, antibody specificities to various toxic gluten-derived peptides were similar to normal patients. Further, no significant binding in any patient group was found to the alpha-gliadin-derived peptides B1342 (Wieser, Belitz & Ashkenazi, 1984) or the 12 amino-acid A-gliadin peptide (Kagnoff, 1985). There appears to be no direct relationship between the toxicities and the antigenic reactivity of gluten-derived peptides. Thus, the intestinal damage in coeliac disease is probably not primarily caused by antibody-dependent mechanisms. The specificities of several monoclonal antibodies which bound to wheat prolamins as well as prolamins from other coeliac-toxic cereals have also been investigated with these toxic gluten-derived peptides, in order to identify possible common epitopes. No monoclonal antibody tested bound the B1342 and 12-amino-acid A-gliadin peptide. However the monoclonal antibodies which were specific for the coeliac-toxic cereal prolamins did show the strongest binding to other coeliac-toxic gluten-derived peptides.
Devery
J M
JM
Division of Plant Industry, North Ryde, Australia.
La Brooy
J T
JT
Krillis
S
S
Davidson
G
G
Skerritt
J H
JH
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000918:Antibody Specificity; D002446:Celiac Disease / Q000276:immunology*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005903:Gliadin / Q000009:adverse effects / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007421:Intestine, Small / Q000276:immunology; D008297:Male; D010455:Peptides / Q000009:adverse effects / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D010455:Peptides; D010940:Plant Proteins; D005903:Gliadin