title: Cytoplasmic localization of human repetitive DNA revealed by in situ hybridization.
abstract: Previously, we showed that a human repetitive DNA sequence (Sau3A family) belonging to a satellite DNA is unstable and constantly excised from the chromosomes (R. Kiyama, H. Matsui, and M. Oishi, 1986, Proc. Natl. Acad. Sci. USA 83, 4665). The unusual property of the repetitive DNA, along with another repetitive DNA (Alu sequence), was further investigated by in situ hybridization in several different human cells including HeLa, bone marrow, and peripheral blood cells. We found that the excised repetitive DNA sequences are localized not only in nuclei, but also in cytoplasm. These results have confirmed the instability of these DNA sequences in the chromosomes and further suggest that the alpha satellite DNA and the Alu sequence which were excised from the chromosomes are released from nuclei to cytoplasm.
authors.affiliation: Institute of Applied Microbiology, University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kiyama
authors.forename: M
authors.initials: M
authors.lastname: Oishi
authors.forename: N
authors.initials: N
authors.lastname: Kanda
mesh_terms: D001853:Bone Marrow / Q000648:ultrastructure; D001854:Bone Marrow Cells; D002467:Cell Nucleus / Q000032:analysis / Q000378:metabolism; D003593:Cytoplasm / Q000032:analysis* / Q000378:metabolism; D004276:DNA, Satellite / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004276:DNA, Satellite