title: Identification of 3-deoxy-manno-2-octulosonic acid, 3-deoxy-5-O-methyl-manno-2-octulosonic acid and 3-deoxy-lyxo-2-heptulosaric acid in the cell wall (theca) of the green alga Tetraselmis striata Butcher (Prasinophyceae).
abstract: The main constituent of the cell wall complex carbohydrate of the scaly green alga Tetraselmis striata Butcher is shown to be 3-deoxy-manno-2-octulosonic acid (42%). In addition two other 2-keto-sugar acids are present, namely, 3-deoxy-5-O-methyl-manno-2-octulosonic acid (7%), the first methylated derivative of 3-deoxy-manno-2-octulosonic acid found in nature, and 3-deoxy-lyxo-2-heptulosaric acid (11%). The characterization of the three 2-keto-sugar acids has been carried out on the corresponding methyl ester methyl glycosides using GLC-MS and 500-MHz 1H-NMR spectroscopy, and on the corresponding reduced alditol acetates using GLC-MS. Other monosaccharides occurring in the cell wall are D-galacturonic acid (14%), D-galactose (4%), D-gulose (2%), D-glucose (1%) and L-arabinose (1%).
authors.affiliation: Botanisches Institut, Universität zu Köln.
authors.forename: B
authors.initials: B
authors.lastname: Becker
authors.forename: K
authors.initials: K
authors.lastname: Hård
authors.forename: M
authors.initials: M
authors.lastname: Melkonian
authors.forename: J P
authors.initials: JP
authors.lastname: Kamerling
authors.forename: J F
authors.initials: JF
authors.lastname: Vliegenthart
mesh_terms: D002473:Cell Wall / Q000032:analysis*; D000460:Chlorophyta / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006027:Glycosides / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008362:Mannosides; D009005:Monosaccharides / Q000032:analysis; D013400:Sugar Acids / Q000302:isolation & purification*
chemical_list: D006027:Glycosides; D008362:Mannosides; D009005:Monosaccharides; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; C060020:3-deoxy-5-O-methylmanno-2-octolusonic acid; C060021:3-deoxy-2-heptulosaric acid