Novel phase-shift marker in cell surface proteins of Bordetella bronchiseptica.
Cell surface proteins of phase I cultures of Bordetella bronchiseptica strains from various species of animals were compared with those of isogenic phase III cultures by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A protein band with a molecular mass of 74 kilodaltons was found only in various phase I cultures. This protein disappeared in parallel with antigenic modulation and had strong antigenicity. We found it to be an appropriate phase-shift marker for two reasons: it was readily extracted and recognized, and it was specifically identified by its distinct antigenicity.
Kuzuya
M
M
Gifu Laboratory, Ghen Corporation, Japan.
Kodama
Y
Y
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001884:Bordetella / Q000032:analysis* / Q000276:immunology / Q000472:pathogenicity; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology; D014774:Virulence
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D008565:Membrane Proteins