Lipoprotein lipase in myocytes and capillary endothelium of heart: immunocytochemical study.
Lipoprotein lipase was immunolocalized by electron microscopy in hearts of young mice; 78% of lipoprotein lipase was in myocytes, 3-6% in extracellular space, and 18% in capillary endothelium. Lipoprotein lipase in myocytes was located primarily in sarcoplasmic reticulum, Golgi sacs, and transport vesicles and also in secretory vesicles at the cell periphery. Lipoprotein lipase in extracellular space was present near the orifice of secretory vesicles of myocytes and in narrow zones spanning the space between myocytes and capillary endothelium. The lowest concentration of lipase associated with endothelial cells was at the basal plasma membrane, whereas the highest concentration was at the surface of luminal projections. Lipoprotein lipase was associated with chylomicrons at the capillary surface but not with chylomicron remnants. Fasting mice for 48 h increased, in heart, lipoprotein lipase activity by 120% and immunolocalized lipase by 270%. The greatest increase (5-fold) occurred at the surface of intraluminal endothelial projections. The findings indicate that lipoprotein lipase in heart is synthesized by myocytes, transferred across extracellular space along cell surfaces and across endothelial cells via vesicles or intracellular channels, and concentrated at the surface of luminal projections of endothelium where the enzyme hydrolyzes triacylglycerol of chylomicrons and very low-density lipoproteins.
Blanchette-Mackie
E J
EJ
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Masuno
H
H
Dwyer
N K
NK
Olivecrona
T
T
Scow
R O
RO
D000818:Animals; D002196:Capillaries / Q000201:enzymology / Q000648:ultrastructure; D003331:Coronary Vessels / Q000201:enzymology / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000201:enzymology* / Q000648:ultrastructure; D005110:Extracellular Space / Q000201:enzymology / Q000648:ultrastructure; D005215:Fasting; D006352:Heart Ventricles / Q000201:enzymology; D007106:Immune Sera; D007150:Immunohistochemistry; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D012016:Reference Values; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000648:ultrastructure
D007106:Immune Sera; D008071:Lipoprotein Lipase