An SV40 mutant oncoprotein has a nuclear location.
T147 is an SV40 mutant that makes a normal small t antigen and a large T antigen that is only 147 amino acids long. We have introduced a second mutation into the genome of T147 which eliminates its ability to encode small t antigen. We show that this double mutant is able to transform C3H10T1/2 mouse cells in a focus assay and F111 rat cells in an agar suspension assay, demonstrating that the transforming domain of T antigen is located within its amino-terminal 147 amino acids. We also show that the T147 mutant T antigen, like wild-type T antigen, has a nuclear location. However, in contrast to wild-type T antigen, which is also found in the plasma membranes of wild-type transformed cells, we fail to detect any mutant T antigen associated with the plasma membranes of T147 transformants.
Sompayrac
L
L
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.
Danna
K J
KJ
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D051379:Mice; D051381:Rats; D013329:Structure-Activity Relationship
D000952:Antigens, Polyomavirus Transforming