title: Pharmacological features of vascular responses of isolated rat common carotid arteries to vasoactive substances revealed by the cannula inserting method.
abstract: Using the cannula inserting method, we studied vascular responses of isolated rat common carotid arteries to 15 vasoactive substances. Intraluminal injections of phenylephrine, norepinephrine (NE), angiotensin II, 5-hydroxytryptamine (5-HT) and prostaglandin F2 alpha (PGF2 alpha) induced a strong vasoconstriction in such a way that a maximum increase in perfusion pressure amounted to approximately 50 mmHg. The order of their potencies were as follows: Phenylephrine greater than or equal to NE greater than or equal to angiotensin II greater than PGF2 alpha greater than or equal to 5-HT much greater than KCl. The selective alpha-2 agonists, xylazine and clonidine, induced nor or a slight vasoconstriction. Tyramine, ATP and acetaldehyde induced only a slight constriction. Histamine, adenosine and acetylcholine (ACh) induced no effect. In preparations preconstricted by a high dose of NE, ACh and isoproterenol induced a vasodilation in a dose-related manner. It is concluded that the cannula inserting method is useful for investigating vascular responses in isolated and perfused rat vessels.
authors.affiliation: Department of Pharmacology, Shinshu University School of Medicine, Matsumoto.
authors.forename: S
authors.initials: S
authors.lastname: Chiba
authors.forename: M
authors.initials: M
authors.lastname: Tsukada
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects; D002404:Catheterization; D015237:Dinoprost / Q000494:pharmacology; D005260:Female; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009638:Norepinephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats
chemical_list: D000804:Angiotensin II; D011189:Potassium Chloride; D015237:Dinoprost; D007545:Isoproterenol; D000109:Acetylcholine; D009638:Norepinephrine