Identification of 'Campylobacter upsaliensis' and other catalase-negative campylobacters from paediatric blood cultures by numerical analysis of electrophoretic protein patterns.
Twenty-one strains of catalase-negative campylobacters from paediatric blood cultures were characterized by one-dimensional SDS-PAGE of cellular proteins. A further 11 Campylobacter strains were included for reference purposes. The partial protein patterns were used as the basis of a numerical analysis, which showed that 17 hippurate-negative strains had a high similarity to 'C. upsaliensis' (r greater than or equal to 0.82) irrespective of their geographical location, and that three hippurate-positive isolates had a high similarity (r greater than or equal to 0.87) to C. jejuni subsp. jejuni. One hippurate-positive CNW strain was not identified. The analysis of SDS-PAGE protein patterns proved an excellent method of characterizing these thermophilic campylobacter as they were difficult to identify by traditional methods.
Owen
R J
RJ
National Collection of Type Cultures, Central Public Health Laboratory, London, U.K.
Morgan
D D
DD
Costas
M
M
Lastovica
A
A
D001426:Bacterial Proteins / Q000032:analysis*; D002167:Campylobacter / Q000032:analysis* / Q000145:classification / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000097:blood / Q000382:microbiology; D002374:Catalase / Q000032:analysis; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D006626:Hippurates / Q000032:analysis; D006801:Humans; D013045:Species Specificity
D001426:Bacterial Proteins; D006626:Hippurates; D002374:Catalase; C030514:hippuric acid