title: Structure and function of the androgen receptor.
abstract: The androgen receptor in several species (human, rat, calf) is a monomeric protein with a molecular mass of 100-110 kDa. The steroid binding domain is confined to a region of 30 kDa, while the DNA-binding domain has the size of approx. 10 kDa. A 40 kDa fragment containing both the DNA and steroid binding domain displayed a higher DNA binding activity than did the intact 100 kDa molecule. cDNA encoding the major part of the human androgen receptor was isolated. The cDNA contains an open reading frame of 2,277 bp but still lacks part of the 5'-coding sequence. Homology with the progesterone and glucocorticoid receptor was about 80% in the DNA binding domain and 50% in the steroid binding domain. The present data provide evidence that the androgen receptor belongs to the superfamily of ligand responsive transcriptional regulators and consists of three distinct domains each with a specialized function.
authors.affiliation: Department of Biochemistry II, Erasmus University, Rotterdam, The Netherlands.
authors.forename: A O
authors.initials: AO
authors.lastname: Brinkmann
authors.forename: P
authors.initials: P
authors.lastname: Klaasen
authors.forename: G G
authors.initials: GG
authors.lastname: Kuiper
authors.forename: J A
authors.initials: JA
authors.lastname: van der Korput
authors.forename: J
authors.initials: J
authors.lastname: Bolt
authors.forename: W
authors.initials: W
authors.lastname: de Boer
authors.forename: A
authors.initials: A
authors.lastname: Smit
authors.forename: P W
authors.initials: PW
authors.lastname: Faber
authors.forename: H C
authors.initials: HC
authors.lastname: van Rooij
authors.forename: A
authors.initials: A
authors.lastname: Geurts van Kessel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008970:Molecular Weight; D051381:Rats; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*
chemical_list: D011944:Receptors, Androgen; D004247:DNA