title: A rat Sertoli cell factor similar to basic fibroblast growth factor increases c-fos messenger ribonucleic acid in cultured Sertoli cells.
abstract: We have shown that the cultured Sertoli cell from the immature rat contains a fibroblast growth factor (FGF)-like factor. It behaves as a cationic peptide, is a potent competence factor for BALB/c3T3 mouse embryo fibroblasts, and displays a high affinity for heparin. Both bovine basic FGF and Sertoli cell FGF-like factor rapidly increase c-fos mRNA in cultured Sertoli cells. FSH, serum, and phorbol esters individually stimulate c-fos in cultured Sertoli cells whereas platelet-derived growth factor, epidermal growth factor, and insulin-like growth factor-I have little affect. However, unlike FSH, basic FGF does not stimulate an increase in cAMP and unlike either serum or phorbol esters, basic FGF does not stimulate phosphoinositol turnover or intracellular calcium changes. When Sertoli cell protein kinase C activity is suppressed by preexposure to phorbol ester, basic FGF continues to be a potent stimulator of c-fos, indicating that the calcium/phospholipid pathway is not involved in FGF induction. Basic FGF and FSH also increase jun-B mRNA levels in cultured Sertoli cells. In response to FGF, jun-B is more transiently increased than c-fos. In contrast, in response to FSH, jun-B persists longer than c-fos. These results indicate that cultured Sertoli cells contain a FGF-like factor that increases c-fos mRNA via a mechanism not involving cAMP and the calcium/phospholipid pathways. The different responsiveness of c-fos and jun-B to FSH and basic FGF may explain differences in the ultimate actions of these two ligands.
authors.affiliation: Department of Pediatrics, Biochemistry and Physiology, University of North Carolina, Chapel Hill 27514.
authors.forename: E P
authors.initials: EP
authors.lastname: Smith
authors.forename: S H
authors.initials: SH
authors.lastname: Hall
authors.forename: L
authors.initials: L
authors.lastname: Monaco
authors.forename: F S
authors.initials: FS
authors.lastname: French
authors.forename: E M
authors.initials: EM
authors.lastname: Wilson
authors.forename: M
authors.initials: M
authors.lastname: Conti
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000378:metabolism / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D005346:Fibroblast Growth Factors