Amniotic fluid phospholipids. Study of the cold acetone precipitation effect.
Amniotic fluid phospholipids were extracted and separated with chloroform: methanol (2:1 by vol). These phospholipids were also precipitated with cold acetone and the percentage of each phospholipid precipitated was calculated. The precipitation percentage of the six phospholipids studied ranged from 33.78 +/- 0.70 (mean +/- SEM) for lecithin to 93.77 +/- 1.40 for phosphatidylserine. The variation of L/S, PG/S and PI/S ratios depending on the precipitation step was also studied. We observed that cold acetone precipitation decreased L/S and PG/S ratios, whereas PI/S remained unchanged. Our results indicated that: (1) cold acetone precipitation affected each phospholipid in a different way; (2) the precipitation step was not an effective way of completely separating surfactant and non-surfactant material; (3) a new predictive value should be established if the L/S ratio without precipitation is to be used in the clinic on a daily basis.
Budria
R J
RJ
Department of Organic Chemistry, University of Zaragoza, Spain.
Gonzalez
F E
FE
Sanz
H M
HM
Ostáriz
S J
SJ
D000096:Acetone; D000653:Amniotic Fluid / Q000032:analysis*; D011232:Chemical Precipitation; D005260:Female; D006801:Humans; D010712:Phosphatidic Acids / Q000032:analysis / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D011247:Pregnancy; D012044:Regression Analysis; D013109:Sphingomyelins / Q000032:analysis / Q000302:isolation & purification*
D010712:Phosphatidic Acids; D013109:Sphingomyelins; D000096:Acetone