title: Comparison of microscale ultracentrifugation and equilibrium dialysis for the determination of the unbound fraction of theophylline in human serum.
abstract: A microscale ultracentrifugation procedure for the measurement of free theophylline in serum is described and evaluated by comparison with an equilibrium dialysis method. Provided an ultracentrifuge is available, micro-ultracentrifugation is a simple, cost-effective technique for the routine determination of free drug fractions.
authors.affiliation: Department of Biochemistry, Royal Infirmary, Glasgow, UK.
authors.forename: K
authors.initials: K
authors.lastname: McKenzie
authors.forename: I D
authors.initials: ID
authors.lastname: Watson
authors.forename: M J
authors.initials: MJ
authors.lastname: Stewart
mesh_terms: D003365:Costs and Cost Analysis; D003956:Dialysis / Q000379:methods; D006801:Humans; D011485:Protein Binding; D013806:Theophylline / Q000097:blood*; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D013806:Theophylline