[Study of oligosaccharides in human urothelial membrane by means of lectins].
We have used four lectins (PHA-L, HPA, RCA-120 and PNA) to characterise the oligosaccharides present in the membrane of the different cell layers of the human vesical urothelium, obtained from 27 normal biopsies of vesical mucus. In all the patients studied the PHA-L and HPA lectins showed an affinity for all the urothelial layers, and a progressive increase in fluorescence is observed from the basal to the surface stratum. The pattern observed when using lectin RCA-120 was a positive marking of all the urothelial layers studied, with a homogeneous intensity in all of them. The PNA lectin did not show reactivity to the normal urothelium in none of the cases studied. We comment on the applications of this type of study both for characterizing the glycid composition of the urothelial cell membrane and for ascertaining its variations in accordance with cell differentiation.
Ferrando
F
F
Sánchez
M C
MC
Gil Salom
M
M
Chuan
P
P
Ramírez
A A
AA
Carretero
P
P
Castells
A
A
D000116:Acetylgalactosamine / Q000032:analysis*; D000368:Aged; D000369:Aged, 80 and over; D002462:Cell Membrane / Q000032:analysis; D005260:Female; D005688:Galactosamine / Q000031:analogs & derivatives*; D005690:Galactose / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins; D008297:Male; D008875:Middle Aged; D001743:Urinary Bladder / Q000032:analysis* / Q000166:cytology
D037102:Lectins; D005688:Galactosamine; D000116:Acetylgalactosamine; D005690:Galactose
Estudio de los oligosacáridos de la membrana del urotelio humano mediante lectinas.