Opsonic activity of ascitic fluids by a chemiluminescent method.
Cirrhotic ascites are highly susceptible to spontaneous bacterial infection, whereas carcinogenic ascites are seldom infected. This difference may be explained by differences in their chemotactic, bactericidal and/or opsomic activities. We measured the chemotactic and opsonic activity of ascitic fluids from 35 alcoholic cirrhotic ascites and of 12 peritoneal carcinogenic ascites. Chemotactic activity was measured by the under-agarose technique and opsonic activity by a luminol-enhanced method. Ascitic fluids from alcoholic cirrhosis had low chemotactic (62 +/- 24.5% that of N-formylated peptide) and opsonic (67 +/- 50% of normal serum) activities on normal human neutrophils. In contrast, ascitic fluids from peritoneal carcinoma were found to possess high opsonic activity (114 +/- 49% of normal serum) and chemotactic activity similar to that of N-formylated peptide. During a 3-month follow-up, 11 spontaneous bacterial infections were observed among the first group against none in the carcinogenic group.
Huu
T P
TP
Institut National de la Santé et de la Recherche Médicale, U. 294, CHU Xavier Bichat, Paris, France.
Bendahou
M
M
Sourbier
P
P
Mal
F
F
Beaugrand
M
M
Hakim
J
J
D001201:Ascites / Q000276:immunology*; D006801:Humans; D008104:Liver Cirrhosis, Alcoholic / Q000276:immunology; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology*; D010534:Peritoneal Neoplasms / Q000276:immunology; D010587:Phagocytosis
D009895:Opsonin Proteins