[The role of adenine nucleotide catabolism in the interphase death of thymocytes, caused by papaverine and dipyridamole].
Papaverine and dipyridamole induce the interphase death of thymocytes rapidly growing four hours later and reaching its maximum by the seventh-eighth hour of the cell incubation. To induce death of thymocytes no constant presence of these preparations in the incubation medium is needed, a definite (for each of preparations) time of the contact with cells being enough. The interphase death of thymocytes induced by papaverine and dipyridamole is preceded by acceleration of the release of adenine nucleotide catabolism products from cells mainly as hypoxanthine and inosine, respectively. These both processes are induced by papaverine for a shorter period of its incubation with cells than by dipyridamole and the joined use of these substances intensifies the above processes. The analysis of the data obtained indicates that thymocytes under the effect of papaverine die rather from the exhaustion of the adenine nucleotide pool, than from a decrease in the adenylate charge of cells. Exogenous adenosine essentially removes the toxic effect of papaverine but not of dipyridamole. Addition of adenine and inosine to thymocytes does not affect their survival rate in the presence of the preparations under study.
Dmitrenko
N P
NP
Goroshnikova
T V
TV
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004176:Dipyridamole / Q000494:pharmacology*; D007399:Interphase / Q000187:drug effects; D010208:Papaverine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D000227:Adenine Nucleotides; D004176:Dipyridamole; D010208:Papaverine
Rol' katabolizma adeninnukleotidov v interfaznoĭ gibeli timotsitov, vyzvannoĭ papaverinom i dipiridamolom.