title: Cloning and sequencing of the human nucleolin cDNA.
abstract: A cDNA containing the entire coding region for human nucleolin has been isolated from a lambda gt10 human retinal library using a bovine cDNA probe. The cDNA hybridized to a transcript of 3000 bases from fast-dividing cells, as well as terminally differentiated tissues of several species. Translation of the nucleotide sequence revealed a long open reading frame which predicts a 707 amino acid protein with several distinct domains. These include repeating elements, four conserved RNA-binding regions, a glycine-rich carboxy-terminal domain and sites for phosphorylation, glycosylation and dibasic cleavage. Human and bovine nucleolin exhibited more additions and/or substitutions of aspartate, glutamate and serine residues in the chromatin-binding domains by comparison with the hamster and mouse nucleolins. These differences may be related to species-specific functions in transcription.
authors.affiliation: Laboratory of Cell Biology and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20892.
authors.forename: M
authors.initials: M
authors.lastname: Srivastava
authors.forename: P J
authors.initials: PJ
authors.lastname: Fleming
authors.forename: H B
authors.initials: HB
authors.lastname: Pollard
authors.forename: A L
authors.initials: AL
authors.lastname: Burns
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D015183:Restriction Mapping; D012160:Retina / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D000097796:Nucleolin
chemical_list: D004247:DNA; D009687:Nuclear Proteins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin