Reaction of o-phthalaldehyde with amino acids and glutathione. Application to high-performance liquid chromatography determination.
Experimental conditions were found for the preparation of stable fluorescent adducts of o-phthalaldehyde with glutathione and its metabolites: glutamine, glutamic and aspartic acids, gamma-glutamylglutamine and gamma-glutamylglutamylglutamine. The structure of the glutathione isoindole derivative obtained was confirmed by NMR studies. The procedure was applied to reversed-phase high-performance liquid chromatographic separation of the previous compounds. The method was extended to glutathione and "total glutathione" determinations.
Morineau
G
G
UA-CNRS 484, Faculté de Pharmacie, Paris, France.
Azoulay
M
M
Frappier
F
F
D000447:Aldehydes; D000596:Amino Acids; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione; D013050:Spectrometry, Fluorescence; D009764:o-Phthalaldehyde
D000447:Aldehydes; D000596:Amino Acids; D009764:o-Phthalaldehyde; D005978:Glutathione