title: Crystallization and preliminary X-ray diffraction studies of oxidized flavodoxin from Chondrus crispus, a red alga.
abstract: Crystals of the oxidized form of flavodoxin from a red alga, Chondrus crispus, have been grown in ammonium sulfate solution by the dialysis method. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 63.6, b = 48.8, and c = 56.8 A. The asymmetric unit contains one molecule of flavodoxin. The crystals diffract X-rays to about 2.0 A resolution and are stable to X-ray beams. The diffraction patterns changed significantly upon soaking the crystal in a solution of a platinum complex. The major heavy-atom sites in the platinum derivative crystal have been identified from the difference Patterson function calculated at 4 A resolution.
authors.affiliation: Department of Biology, Faculty of Science, Osaka University.
authors.forename: K
authors.initials: K
authors.lastname: Fukuyama
authors.forename: H
authors.initials: H
authors.lastname: Matsubara
authors.forename: Y
authors.initials: Y
authors.lastname: Katsube
authors.forename: L J
authors.initials: LJ
authors.lastname: Rogers
mesh_terms: D003460:Crystallization; D005418:Flavodoxin / Q000032:analysis*; D005420:Flavoproteins / Q000032:analysis*; D010084:Oxidation-Reduction; D000461:Rhodophyta / Q000032:analysis*; D014961:X-Ray Diffraction
chemical_list: D005418:Flavodoxin; D005420:Flavoproteins