title: Enzyme assay of L-myo-inositol 1-phosphate synthetase based on high-performance liquid chromatography of benzoylated inositol.
abstract: A procedure for the determination of inositol by reversed-phase HPLC is described which is based on a precolumn benzoylation and detection at 230 nm. This procedure was used to assay the activity of L-myo-inositol 1-phosphate synthetase (EC 5.5.1.4) after treatment of the enzymatic product by a phosphatase.
authors.affiliation: Ag. Enzymologie, GBF, Gesellschaft für Biotechnologische Forschung, Braunschweig, Federal Republic of Germany.
authors.forename: F
authors.initials: F
authors.lastname: Feth
authors.forename: R
authors.initials: R
authors.lastname: Wagner
authors.forename: H
authors.initials: H
authors.lastname: Baydoun
authors.forename: K G
authors.initials: KG
authors.lastname: Wagner
mesh_terms: D001565:Benzoates / Q000032:analysis*; D002238:Carbohydrate Epimerases / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007294:Inositol / Q000031:analogs & derivatives* / Q000032:analysis; D007296:Myo-Inositol-1-Phosphate Synthase / Q000032:analysis*; D015003:Yeasts / Q000201:enzymology
chemical_list: D001565:Benzoates; D007294:Inositol; D002238:Carbohydrate Epimerases; D007296:Myo-Inositol-1-Phosphate Synthase