title: Development and application of immunoassay for paraquat: radioimmunoassay.
abstract: A sensitive radioimmunoassay for paraquat is reported. Anti-paraquat antisera were produced by repeated immunization in rabbits with 1-methyl, 1'-hexanoic acid-4,4'-bipyridinium (MHBP) coupled to bovine serum albumin (BSA). Less than 0.5 ng of paraquat dichloride was detectable by this assay system. These antisera were strongly cross-reactive with the bipyridyl ring and methyl group in either the 1- or 1'-position of paraquat. The determination of paraquat in tissues of paraquat-poisoned cadavers was also carried out.
authors.affiliation: Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: T
authors.initials: T
authors.lastname: Takatori
authors.forename: K
authors.initials: K
authors.lastname: Terazawa
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.forename: C
authors.initials: C
authors.lastname: Wakasugi
authors.forename: M
authors.initials: M
authors.lastname: Masui
authors.forename: H
authors.initials: H
authors.lastname: Ikeda
mesh_terms: D001344:Autopsy; D003124:Colorimetry; D003429:Cross Reactions; D006801:Humans; D010269:Paraquat / Q000032:analysis* / Q000276:immunology / Q000506:poisoning; D011863:Radioimmunoassay; D014018:Tissue Distribution
chemical_list: D010269:Paraquat