HPLC separation and characterization of chlorin derivatives with intact ring V from acid degradation products of silkworm excrement crude chlorophyll mixture.
A reversed-phase HPLC system with 88% methanol in 1 M ammonium acetate buffer pH 5.35 as the mobile phase on an ODS column was used to analyse pheophorbides a, a', b, b' and pyropheophorbides a and b. Pyropheophorbides a and b were found in samples obtained from silkworm excrement but not from spinach leaves though the preparation methods used were the same. This difference suggests that chlorophylls undergo metabolism in the body of silkworm to give pyrochlorophyll derivatives.
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Laboratory for Antimalarial Drug Research, Second Military Medical College, People's Republic of China.
Xu
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D000818:Animals; D012831:Bombyx / Q000378:metabolism*; D002734:Chlorophyll / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D011166:Porphyrins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D011166:Porphyrins; D002734:Chlorophyll; C006969:chlorin