In vitro investigation of the priming principle for rapid neuromuscular block.
The priming principle was investigated in the rat phrenic nerve-diaphragm preparation stimulated continuously at 0.2 Hz. Tubocurarine was added to the organ bath as either a single (non-primed) or a divided (primed) dose. Priming consisted of 15% or 20% of the final dose, with priming intervals of 5 or 10 min. Priming decreased significantly the time to 80% block and was associated with mild neuromuscular block. A simple model adequately predicted the time to 50% and 80% block, using the same diffusion constant for both primed and non-primed conditions. The onset of neuromuscular block, with and without priming, depended mostly upon the distribution of the drug to its site(s) of action.
Storella
R J
RJ
Department of Anesthesiology, Hahnemann University, Philadelphia, Pa 19102.
Jaffe
J
J
Mehr
E
E
Rosenberg
H
H
D000818:Animals; D003964:Diaphragm / Q000187:drug effects; D004334:Drug Administration Schedule; D008297:Male; D009469:Neuromuscular Junction / Q000187:drug effects*; D010791:Phrenic Nerve / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014403:Tubocurarine / Q000008:administration & dosage* / Q000494:pharmacology
D014403:Tubocurarine