Expression of the thyroid sodium/iodide symporter in Xenopus laevis oocytes.
Poly(A+) RNA isolated from FRTL-5 cells (a continuous line of cultured and fully functional rat thyroid cells (Ambesi-Impiombato, F. S., Parks, L. A. M., and Coons, H. G. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 3455-3459] was injected into Xenopus laevis oocytes, and the expression of the Na+/I- symporter in the plasma membrane was assayed by measuring the Na+-dependent ClO4--sensitive uptake of 125I. Expression of the Na+/I- symporter was detected as a 7-fold average increase in transport over background, 5-6 days after injection. Poly(A+) RNA was subsequently fractionated by sucrose gradient centrifugation, and fractions were assayed for their ability to induce I- transport activity. The poly(A+) RNA encoding the Na+/I- symporter was found in a fraction containing messages of 2.8-4.0 kilobases in length.
Vilijn
F
F
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461.
Carrasco
N
N
D000818:Animals; D001692:Biological Transport; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D005260:Female; D007454:Iodides / Q000378:metabolism*; D009865:Oocytes; D011061:Poly A / Q000235:genetics; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism*; D014982:Xenopus laevis
D002352:Carrier Proteins; D007454:Iodides; D011061:Poly A; D012964:Sodium