title: The diagnosis of human hydatidosis by measurement of specific IgE antibody by enzyme immunoassay.
abstract: The sensitivity and specificity of an enzyme immunosorbent assay (EIA) for measurement of anti-Echinococcus granulosus specific IgE antibody using microtiter strips was evaluated. The sensitivity was 92.2% for 90 patients with cysts in the liver or other body sites but excluding the patients with cysts in the lungs or bones. In this latter group (n = 26) a sensitivity of 61.5% was recorded. The specificity for control groups comprising 89 blood bank sera from Spain and 48 sera from atopic Swedes was 100%. Patients infected with other parasites (n = 78) were usually negative (81%). 11 of the 15 false positives were found in patients with total IgE greater than 5,000 kU/l. The microtiter EIA method employed can be considered as a very convenient method to be included in the range of immunodiagnostic tests for human hydatid disease.
authors.affiliation: Pharmacia Diagnostics AB, Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Sjölander
authors.forename: J A
authors.initials: JA
authors.lastname: Guisantes
authors.forename: J M
authors.initials: JM
authors.lastname: Torres-Rodriguez
authors.forename: H
authors.initials: H
authors.lastname: Schröder
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000918:Antibody Specificity; D002648:Child; D004443:Echinococcosis / Q000175:diagnosis* / Q000276:immunology; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests
chemical_list: D000909:Antibodies, Helminth; D007073:Immunoglobulin E