Development of hexachlorobenzene porphyria in rats: time sequence and relationship with lipid peroxidation.
The relationship between the development of porphyria and free-radical formation induced by hexachlorobenzene was studied in iron-overloaded rats. The first sign of porphyria, an increase in porphyrins in the liver, was detected at day 22. Liver malondialdehyde was also increased at day 22. During the following weeks, liver porphyrins and malondialdehyde increased simultaneously, accompanied by a decrease in uroporphyrinogen decarboxylase activity and glucose-6-phosphate activity in liver, and a high excretion of porphyrins in the urine. In the rats given hexachlorobenzene, changes were detected in the pattern of lipids in the liver microsomes. In comparison with the controls, there were decreases in C20:4 and C22:5 fatty acids, whereas the fatty acid C20:3w6 was increased. In this study of hexachlorobenzene-induced liver damage there was no difference in the time course of the development of porphyria and that of lipid peroxidation.
Visser
O
O
Department of Internal Medicine II, Erasmus University Rotterdam, The Netherlands.
van den Berg
J W
JW
Edixhoven-Bosdijk
A
A
Koole-Lesuis
R
R
Rietveld
T
T
Wilson
J H
JH
D000818:Animals; D002722:Chlorobenzenes / Q000633:toxicity*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005609:Free Radicals; D006581:Hexachlorobenzene / Q000633:toxicity*; D015227:Lipid Peroxidation; D008099:Liver / Q000032:analysis / Q000201:enzymology; D008862:Microsomes, Liver / Q000032:analysis; D011164:Porphyrias / Q000139:chemically induced* / Q000209:etiology; D011166:Porphyrins / Q000032:analysis / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014575:Uroporphyrinogen Decarboxylase / Q000032:analysis
D002722:Chlorobenzenes; D005227:Fatty Acids; D005609:Free Radicals; D011166:Porphyrins; D006581:Hexachlorobenzene; D014575:Uroporphyrinogen Decarboxylase