Adipokinetic hormone gene sequence from Manduca sexta.
A gene sequence encoding the neuropeptide adipokinetic hormone (AKH) was isolated from a genomic library of the tobacco hornworm, Manduca sexta. 2.5 x 10(5) recombinant plaques were screened with a 29-mer oligonucleotide substituted with deoxyinosine at six ambiguous codon positions. One clone was obtained from which we deduced an intronless gene encoding the AKH precursor (prepro-AKH). From NH2 to COOH terminus, prepro-AKH consists of a signal peptide, a single AKH peptide block followed by a Gly-Lys-Arg processing site, and a 34-residue sequence that appears unrelated to known peptides. The cloned AKH gene sequence hybridized to one transcript from the adult corpus cardiacum/corpus allatum complex. The Manduca genome appears to contain one copy of the AKH gene. These studies are a basis for explaining synthesis of a representative from a widespread, physiologically important family of arthropod neuropeptides.
Bradfield
J Y
JY
Department of Entomology, Texas A & M University, College Station 77843-2475.
Keeley
L L
LL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005796:Genes; D007301:Insect Hormones / Q000235:genetics* / Q000302:isolation & purification; D007915:Lepidoptera / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009842:Oligopeptides / Q000235:genetics* / Q000302:isolation & purification; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D015183:Restriction Mapping; D014158:Transcription, Genetic
D007301:Insect Hormones; D009842:Oligopeptides; C044833:adipokinetic hormone; D011761:Pyrrolidonecarboxylic Acid