Chloroplast biogenesis: quantitative determination of monovinyl and divinyl chlorophyll(ide) a and b by spectrofluorometry.
Simultaneous equations for the determination of monovinyl (MV) and divinyl (DV) Chl a and b by spectrofluorometry in unsegregated mixtures of these tetrapyrroles were derived. The same equations can also be used for the quantitative determination of MV and DV chlorophyllide (Chlide) a and b in unpurified mixtures, after extraction of the Chls in hexane. The equations used differences in the Soret excitation maxima of these tetrapyrroles in ether at 77 degrees K, in order to correct the Soret excitation overlap between MV and DV Chl(ide) a and between MV and DV Chl(ide) b.
Wu
S M
SM
Laboratory of Plant Pigment Biochemistry and Photobiology, University of Illinois, Urbana 61801.
Mayasich
J M
JM
Rebeiz
C A
CA
D002734:Chlorophyll / Q000031:analogs & derivatives*; D002735:Chlorophyllides / Q000032:analysis* / Q000096:biosynthesis; D002736:Chloroplasts / Q000378:metabolism*; D008433:Mathematics; D013050:Spectrometry, Fluorescence
D002735:Chlorophyllides; D002734:Chlorophyll