[Monoclonal antibodies raised against cultured cells and their application in cancer diagnosis].
Since cultured cells have the possibility to produce tumor-associated substances in vitro, we generated several monoclonal antibodies using cultured cells as immunogen and applied them to cancer diagnosis. Two monoclonal antibodies (F602-1 and F602-6) were generated against a newly established ovarian mesonephroid cancer cell line (RMG-II), and a double determinants sandwich enzyme-immunoassay system was developed. Their epitopes were proved to exist on the CA125 molecules, but to be different from the one recognized by OC125, and almost the same result of serum assay as CA125 was obtained by this assay. A new monoclonal antibody (MSN-1) was produced by immunizing a new endometrial cancer cell line (SNG-II). MSN-1 recognized the Lewis-b carbohydrate moiety on the cell surface glycolipid, and seldom reacted immunohistochemically with normal endometrium, but reacted with about 90% of endometrial cancer cases. By application of MSN-1 to flow-cytometry, the possibility of differentiating endometrial normal cells from the cancer cells was demonstrated.
Nozawa
S
S
Dept. of Obstetrics and Gynecology, Keio University School of Medicine.
Udagawa
Y
Y
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002460:Cell Line; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008649:Mesonephroma / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000276:immunology*; D014594:Uterine Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal