title: Fatty acid acylation of the fusion glycoprotein of human respiratory syncytial virus.
abstract: We describe the covalent attachment of palmitate to the fusion glycoprotein of respiratory syncytial virus and the identification of the attachment site. Labeling of respiratory syncytial virus-infected Vero cells with [3H]palmitate, followed by the purification and subsequent analysis of the fusion glycoprotein in conjunction with polyacrylamide gel electrophoresis, demonstrated that the fatty acid is covalently attached to the F1 subunit of the fusion glycoprotein. The bound palmitate was sensitive to 1 M hydroxylamine at neutral pH. In addition, the release of palmitate label by reduction with sodium borohydride showed that the palmitate is linked to the protein through a thioester bond. Isolation of a radiolabeled peptide from a tryptic digest of the protein and subsequent amino-terminal sequence analysis revealed that the cysteine residue (amino acid residue 550) within the anchor sequence, located at the carboxyl terminus of the F1 subunit, is the covalent attachment site for palmitate.
authors.affiliation: Praxis Biologics, Inc., Rochester, New York 14623.
authors.forename: R G
authors.initials: RG
authors.lastname: Arumugham
authors.forename: R C
authors.initials: RC
authors.lastname: Seid
authors.forename: S
authors.initials: S
authors.lastname: Doyle
authors.forename: S W
authors.initials: SW
authors.lastname: Hildreth
authors.forename: P R
authors.initials: PR
authors.lastname: Paradiso
mesh_terms: D000215:Acylation; D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification; D015707:HN Protein; D008969:Molecular Sequence Data; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000378:metabolism*; D014759:Viral Envelope Proteins; D014764:Viral Proteins
chemical_list: D000956:Antigens, Viral; D015707:HN Protein; D010169:Palmitic Acids; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G; D019308:Palmitic Acid