The alternatively spliced exon of the platelet-derived growth factor A chain encodes a nuclear targeting signal.
We have previously shown that the SIS/platelet-derived growth factor B chain contains a nuclear targeting signal near its C terminus. Here we show that the platelet-derived growth factor A chain also contains a nuclear targeting signal encoded by an exon which is subject to alternative splicing. This sequence is capable of targeting a nonsecreted form of the A chain to the nucleus and can also target the cytoplasmic proteins dihydrofolate reductase, chloramphenicol acetyltransferase, and pyruvate kinase to the nucleus.
Maher
D W
DW
Department of Chemistry, University of California--San Diego, La Jolla 92093.
Lee
B A
BA
Donoghue
D J
DJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005091:Exons; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D012326:RNA Splicing; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism
D010982:Platelet-Derived Growth Factor; D013762:Tetrahydrofolate Dehydrogenase; D015500:Chloramphenicol O-Acetyltransferase; D011770:Pyruvate Kinase