Complement-mediated enhancement of IgA-induced H2O2 release by human polymorphonuclear leucocytes.
In a previous study we have demonstrated that heat-killed Staphylococcus aureus opsonized with either purified human serum IgA or secretory IgA (sIgA) can induce a respiratory burst (measured as H2O2 release) in human polymorphonuclear leucocytes (PMN; Gorter et al., 1987). In the present study we have investigated whether opsonization of IgA-coated staphylococci with complement has an additional effect on the H2O2 release of PMN. It was demonstrated that staphylococci coated with IgA (or sIgA) and subsequently opsonized with complement induced at least a two-fold increase in the specific H2O2 release compared with bacteria coated with IgA (or sIgA) alone (P less than 0.05 and P less than 0.02, respectively). The co-operative effect of IgA and complement was also observed in the presence of 10 mM ethyleneglycoltetraacetic acid containing 5 mM MgCl2 (MgEGTA), suggesting that activation of the alternative pathway of complement is sufficient to exert this effect. Using D-deficient serum as a source of complement we could demonstrate that activation of the alternative pathway is essential for the co-operative effect of complement and IgA. The increase in specific H2O2 release caused by complement was found to be dependent on the amount of IgA initially used to opsonize the bacteria. Finally the co-operative effect of IgA and complement was not restricted to one IgA subclass, because an additional opsonization of S. aureus coated with sIgA1 or sIgA2 with complement resulted in both cases in a statistically significant enhanced specific H2O2 release by PMN (P less than 0.05).
Gorter
A
A
Department of Nephrology, University Hospital, Leiden, The Netherlands.
Hiemstra
P S
PS
Leijh
P C
PC
van der Sluys
M E
ME
van den Barselaar
M T
MT
Van Es
L A
LA
Daha
M R
MR
D003176:Complement C3 / Q000502:physiology; D003170:Complement Pathway, Alternative; D003165:Complement System Proteins / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007070:Immunoglobulin A / Q000502:physiology*; D009504:Neutrophils / Q000378:metabolism*; D010101:Oxygen Consumption
D003176:Complement C3; D007070:Immunoglobulin A; D003165:Complement System Proteins; D006861:Hydrogen Peroxide