In vitro stability of the immunoreactivity of the ovarian cancer-associated antigen CA 125.
A commercially available two-site solid phase radio-immunoassay was used to study the influence of time and temperature on the in vitro stability of CA 125. In whole blood samples stored at 20 degrees C the CA 125 immunoreactivity increased significantly after 2 days of storage. However, serum could be stored at 20 degrees C for 3 days and at -20 degrees C and -80 degrees C for three weeks without statistically significant changes in the CA 125 immunoreactivity. Therefore, for storage up to 3 days at 20 degrees C, serum should be preferred. For prolonged storage serum samples should be kept at -20 degrees C or -80 degrees C.
Mogensen
O
O
Department of Oncology, Aarhus University Hospital, Denmark.
Mogensen
B
B
Jakobsen
A
A
D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis* / Q000276:immunology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D011863:Radioimmunoassay; D013696:Temperature; D013997:Time Factors
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; C017589:ovarian tumor associated antigen