Solubilization of membrane-associated cross-protection factor(s) of Pasteurella multocida.
Pasteurella multocida harvested from the blood of turkeys dying of experimental fowl cholera were purified by centrifugation and lysed. The soluble and membrane-associated components of the bacteria were separated by centrifugation. Nonionic (octylglucoside) and zwitterionic (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate; CHAPS) detergents were tested for their abilities to solubilize the cross-protection factor(s) (CPF) from the membrane-associated component. Protection studies in turkeys showed that optimum solubilization was by 1.0% octylglucoside and 0.5% CHAPS. Antibodies from turkeys made against solubilized membrane-associated CPF passively cross-protected poults against challenge. Ion exchange chromatography of detergent-solubilized CPF resulted in elution of two protein-containing peaks, each of which conferred active immune protection.
Rimler
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RB
National Animal Disease Center, Ames, Iowa 50010.
Rhoades
K R
KR
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D001428:Bacterial Vaccines / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D007110:Immunity, Active; D007116:Immunization, Passive / Q000662:veterinary; D010325:Pasteurella / Q000276:immunology* / Q000302:isolation & purification; D010326:Pasteurella Infections / Q000276:immunology / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology*; D018805:Sepsis / Q000276:immunology / Q000662:veterinary; D012995:Solubility; D014422:Turkeys / Q000276:immunology*
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines