Isolation of line 10 hepatoma-cell membrane by the water extraction method and immunochemical analysis.
Water extraction method was applied to isolate the cell membrane from line 10 hepatoma cells and normal liver cells in strain 2 guinea pig. The materials isolated by this method were further analyzed by different immunochemical techniques including SDS-PAGE, crossed immunoelectrophoresis and crossed affino immunoelectrophoresis to demonstrate the major components and their antigenicities. Five major glycoproteins of apparent molecular weights of 44, 46, 62, 64, and 68 kDa were prominent in line 10 tumor cell materials, whereas one band of molecular weight of 82 kDa was prominent in the materials from normal liver cells. Also four minor components from line 10 tumor cells were found to be glycoprotein in nature.
Ahsan
C R
CR
Department of Bacteriology, Hirosaki University School of Medicine, Aomori.
Sasaki
J
J
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000276:immunology; D003208:Concanavalin A / Q000031:analogs & derivatives; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007123:Immunoelectrophoresis, Two-Dimensional; D008099:Liver / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D012995:Solubility; D014407:Tumor Cells, Cultured; D014867:Water
D000954:Antigens, Surface; D005452:Fluoresceins; D005456:Fluorescent Dyes; C042784:fluorescein isothiocyanate-concanavalin A; D014867:Water; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate