title: Electrically regulated cellular morphological and cytoskeletal changes on an optically transparent electrode.
abstract: Electrically regulated morphological and cytoskeletal changes of HeLa cells were studied on an optically transparent electrode (OTE), on which potential-applied surface cellular behavior and morphogenesis were easily observed. Upon application of a potential, HeLa cells in an OTE exhibited remarkable morphological changes above +0.5 V (vs. Ag/AgCl) and below 0 V. At each potential in this potential range, change in F-actin distribution was observed using a fluorescent probe (rhodamine phalloidin). These results suggest that an electrical field induces a subcellular cytoskeletal change. Electrostimulation of cells with OTE can be a valuable strategy for the manipulation of cultured human cells.
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yaoita
authors.forename: M
authors.initials: M
authors.lastname: Aizawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ikariyama
mesh_terms: D000199:Actins / Q000378:metabolism*; D002462:Cell Membrane / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D004558:Electric Stimulation; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008839:Microelectrodes
chemical_list: D000199:Actins