title: Changes in cell surface antigen expression during murine bone marrow cell regeneration in vivo and proliferation in vitro.
abstract: Modulations in surface antigen expression during marrow regeneration in vivo, and proliferation in vitro in response to haemopoietic growth factors, were studied using a panel of monoclonal antibodies recognizing antigenic determinants expressed by primitive multipotential progenitor cells (Thy-1, Qa-m7), or lineage antigens restricted to committed progenitors and differentiated cells of the neutrophil/macrophage (7/4) and B lymphocyte (B220) lineages. These two categories of antigen exhibited differing responses to marrow perturbation and proliferation. Following administration of a cytotoxic dose of 5-fluorouracil, or lethal irradiation and transplantation of normal donor marrow, the levels of Thy-1 and Qa-m7 antigen expression rapidly increase, reaching a peak at the onset of regeneration: the nadir of marrow cellularity. Expression of these antigens returns to normal as regeneration proceeds and marrow is reconstituted. 7/4 and B220 antigen expression reflect the presence or absence of maturing cells bearing these markers: antigen expression declining following perturbation, and re-emerging during the course of regeneration. In vitro, when marrow cells taken from mice 8 days following treatment with 5-FU are grown in liquid culture in the presence of colony-stimulating factor-1 plus bladder cell carcinoma cell line 5637 conditioned medium, marrow cells are stimulated to proliferate and differentiate along the neutrophil/macrophage lineage. 7/4 antigen expression increases throughout the culture period, and B220 antigen is undetectable after the fifth day of culture. Thy-1 antigen expression also rises and remains elevated, and Qa-m7 antigen expression remains stable.
authors.affiliation: Cell Biology Group, Peter MacCallum Cancer Institute, Melbourne, Vic., Australia.
authors.forename: I
authors.initials: I
authors.lastname: Bertoncello
authors.forename: S H
authors.initials: SH
authors.lastname: Bartelmez
authors.forename: T R
authors.initials: TR
authors.lastname: Bradley
authors.forename: G S
authors.initials: GS
authors.lastname: Hodgson
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D001853:Bone Marrow / Q000276:immunology* / Q000502:physiology; D001854:Bone Marrow Cells; D002455:Cell Division; D005472:Fluorouracil; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D012038:Regeneration / Q000187:drug effects / Q000528:radiation effects
chemical_list: D000943:Antigens, Differentiation; D000954:Antigens, Surface; D005472:Fluorouracil