[Analysis of anti-platelet antibody and platelet volume in idiopathic thrombocytopenic purpura].
We investigated platelets and plasma from patients with idiopathic thrombocytopenic purpura (ITP) to elucidate the antigenic determinants at which their autoantibodies are directed, and studied the relationship between anti-platelet antibody and platelet volume. We used flow cytometry to detect platelet-associated IgG (PAIgG), C3 (PAC3), IgM (PAIgM) and platelet volume, and also to determine the binding rate of monoclonal anti-platelet antibodies in patients with ITP. The following results were obtained. 1. Both anti-GPIIb/IIIa autoantibodies (21 of 71 patients) and anti-GPIb autoantibodies (3 of 71 patients) were found in ITP. 2. The decrease in platelet count in patients without anti-GPIIb/IIIa autoantibodies was significant. 3. The increase in platelet volume was found more frequently in patients with a platelet count less than 50,000 and in untreated patients. 4. There was a positive correlation between the platelet volume and PAIgM in patients with a platelet count less than 30,000 and high levels of PAIgM.
Nomura
S
S
Soga
T
T
Yanabu
M
M
Suzuki
M
M
Kondo
K
K
Sone
N
N
Kitada
C
C
Nagata
H
H
Kokawa
T
T
Yasunaga
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D011696:Purpura, Thrombocytopenic / Q000097:blood / Q000276:immunology*
D001323:Autoantibodies; D001324:Autoantigens; D010980:Platelet Membrane Glycoproteins