Construction and use of a replication-competent human immunodeficiency virus (HIV-1) that expresses the chloramphenicol acetyltransferase enzyme.
The construction and properties of an infectious human immunodeficiency virus (HIV) that expresses the bacterial gene chloramphenicol acetyltransferase are described. This virus can be used in vitro to screen for drugs that inhibit HIV infection. The marked virus may also be used to trace the routes of infection from the site of inoculation in animal experiments.
Terwilliger
E F
EF
Laboratory of Biochemical Pharmacology, Dana-Farber Cancer Institute, Boston, MA 02115.
Godin
B
B
Sodroski
J G
JG
Haseltine
W A
WA
D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D003588:Cytopathogenic Effect, Viral; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006678:HIV / Q000235:genetics* / Q000254:growth & development; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D014779:Virus Replication
D004274:DNA, Recombinant; D015500:Chloramphenicol O-Acetyltransferase