Saturation of human chromosome 3 with unique sequence hybridization probes.
We have generated chromosome 3-specific recombinant libraries in both lambda and cosmid cloning vectors starting with somatic cell hybrids (hamster/human) containing either an intact chromosome 3 or a chromosome 3 with an interstitial deletion removing 75% of long-arm sequences. The libraries contained between 2 X 10(5) and 5 X 10(6) independent recombinants. Approximately 2% of the recombinants in these libraries contain inserts of human DNA. These were identified by hybridizing the recombinants to radioactively labeled total human DNA. Over 2500 recombinants containing human DNA were isolated from these various libraries and DNA was prepared from each of them. This represents 80,000 kb of cloned chromosome 3 sequences. One-third of the DNAs were digested with EcoRI or HindIII, and fragments free of repetitive sequences were radioactively labeled using random hexanucleotide primers and tested as unique sequence hybridization probes. Over 6500 of the fragments were tested and of these 758 were unique sequence probes with minimal or no background hybridization. Their hybridization only to chromosome 3 was verified. These probes, which were derived from 452 independent recombinants, should provide an effective saturation of human chromosome 3.
Smith
D I
DI
Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48201.
Mangrulker
R
R
Geist
R
R
Gilbert
J
J
Kinsman
K
K
Drabkin
H
H
Golembieski
W
W
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D002893:Chromosomes, Human, Pair 3; D006224:Cricetinae; D003412:Cricetulus; D015342:DNA Probes; D004274:DNA, Recombinant; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D015342:DNA Probes; D004274:DNA, Recombinant