[Sulfadimethoxine as a promising drug in the treatment of infections caused by Mycobacterium kansasii and Mycobacterium xenopi--differentiation between M. kansasii and M. marinum and between M. gordonae and M. scrofulaceum by the susceptibility testing to sulfadimethoxine].
Susceptibility testing to sulfadimethoxine of various mycobacteria was made using Ogawa egg medium containing various concentrations of the drug. Each medium was inoculated by a 0.02 ml-sample of bacterial suspensions (10 mg wet weight per ml) prepared from 10 day-old (M. tuberculosis, 14 day-old) cultures growing on Ogawa egg medium after homogenizing the bacteria by shaking with glass beads. The media inoculated were incubated at 37 degrees C for 14 days (M. marinum, at 28 degrees C). The minimal inhibitory concentration (MIC) was determined as the lowest drug concentration on which the growth of bacteria was completely inhibited. However, residual growth occurred often. This was regarded as negative growth, because control medium containing no drug always exhibited abundant membraneous growth. Of the mycobacteria tested, M. kansasii (MICs, 0.8-3.2 micrograms/ml) and M. xenopi (MICs, 0.2-3.2 micrograms/ml) were most susceptible to this drug. Other mycobacteria showed the MICs higher than 3.2 micrograms/ml. The drug seemed to be useful in the treatment of infections caused by M. kansasii and M. xenopi. Furthermore, the susceptibility testing to sulfadimethoxine was considered to be useful for differentiation between two photochromogens, M. kansasii and M. marinum and for differentiation between two scotochromogens, M. scrofulaceum and M. gordonae (Fig. 3 and 4).
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D006801:Humans; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects*; D009164:Mycobacterium Infections / Q000382:microbiology*; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000187:drug effects*; D013412:Sulfadimethoxine / Q000494:pharmacology*
D013412:Sulfadimethoxine