Isolation and preliminary characterization of a 2-chlorobenzoate degrading Pseudomonas.
Pseudomonas sp. strain B-300, which is able to utilize 2-chlorobenzoic acid, was isolated from a soil sample by enrichment culture. This strain was shown to grow on 2-chlorobenzoic acid and to completely degrade the substrate with concomitant chlorine ion release. Concentrations of 2-chlorobenzoic acid higher than 0.5% (w/v) were toxic to the cells. Our study also suggested that in the presence of glucose, 2-chlorobenzoic acid is converted to catechol or chlorocatechol; these are in turn transformed to muconic and chloromuconic acid, respectively, suggesting a repression by glucose of some of the degradation pathway enzymes. A similar scheme was already described for 3-chlorobenzoate degradation by pAC25 plasmid.
Sylvestre
M
M
Centre de recherche microbiologie appliquée, Institut Armand Frappier, Laval, Québec, Canada.
Mailhiot
K
K
Ahmad
D
D
Massé
R
R
D001673:Biodegradation, Environmental; D055598:Chemical Phenomena; D002621:Chemistry; D002723:Chlorobenzoates / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology
D002723:Chlorobenzoates; C060415:2-chlorobenzoic acid