Establishment and characterization of a granulocytic subclone (UM 384) from the monoblastic cell line U 937.
The human cell line U 937 spontaneously expresses monocytic maturation and can be induced into macrophage-like cells when treated with retinoic acid, sodium butyrate, or 2,3-O-tetra decanoylphorbol-13-acetate. We have selected a subclone, designated UM 384, that expresses granulocytic characteristics and can be induced to mature to granulocytes after exposure to retinoic acid, actinomycin D, and dimethylsulfoxide, and to monocyte-like cells when treated with sodium butyrate and phytohemagglutinin-stimulated leukocyte-conditioned medium. These cells retain the same constitutive markers as the parent line including histocompatibility leukocyte antigens and karyotype but share numerous chromosomal abnormalities, mainly t(X;8) (p21;q12).
Champelovier
P
P
Laboratory of Hematology, University Hospital, University Joseph Fourier, Grenoble, France.
Seigneurin
D
D
Leroux
D
D
Micouin
C
C
Kolodie
L
L
D000943:Antigens, Differentiation / Q000032:analysis; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002454:Cell Differentiation; D002999:Clone Cells; D003609:Dactinomycin / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology*; D006651:Histocytochemistry; D006801:Humans; D066298:In Vitro Techniques; D007951:Leukemia, Myeloid / Q000473:pathology*; D008854:Microscopy, Electron; D009000:Monocytes / Q000166:cytology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology
D000943:Antigens, Differentiation; D002087:Butyrates; D020148:Butyric Acid; D003609:Dactinomycin; D014212:Tretinoin