Thin-layer chromatography of free porphyrins for diagnosis of porphyria.
In this simple thin-layer chromatographic (TLC) technique for evaluating porphyrin excretion, porphyrins are extracted from urine or feces, then separated on silica-gel TLC plates. The distinct porphyrin bands are observed by viewing the plates under long-wave fluorescent light. Positive screening tests can readily be confirmed or rejected, and a more comprehensive investigation confidently undertaken.
Henderson
M J
MJ
Department of Chemical Pathology, St. James's University Hospital, Leeds, U.K.
D002855:Chromatography, Thin Layer; D003306:Coproporphyrins / Q000378:metabolism / Q000652:urine; D005243:Feces / Q000032:analysis; D006801:Humans; D011164:Porphyrias / Q000175:diagnosis*; D011166:Porphyrins / Q000378:metabolism* / Q000652:urine; D011524:Protoporphyrins / Q000378:metabolism / Q000652:urine; D014578:Uroporphyrins / Q000378:metabolism / Q000652:urine
D003306:Coproporphyrins; D011166:Porphyrins; D011524:Protoporphyrins; D014578:Uroporphyrins