Formation of highly ordered cross-linked lattices between asparagine-linked oligosaccharides and lectins observed by electron microscopy.
The interaction of asparagine-linked carbohydrates (N-linked) with carbohydrate binding proteins called lectins has been demonstrated to be involved in a variety of cellular recognition processes. Certain N-linked carbohydrates have been shown to be multivalent and capable of binding, cross-linking, and precipitating lectins (Bhattacharyya, L., Ceccarini, C., Lorenzoni, P., and Brewer, C. F. (1987) J. Biol. Chem. 262, 1288-1293; Bhattacharyya, L., Haraldsson, M., and Brewer, C. F. (1987) J. Biol. Chem. 262, 1294-1299; Bhattacharyya, L., Haraldsson, M., and Brewer, C. F. (1988) Biochemistry 27, 1034-1041). Recent data have further suggested that certain oligomannose and bisected hybrid-type N-linked glycopeptides form homogeneous cross-linked lattices with concanavalin A (Bhattacharyya, L., Khan, M. I., and Brewer, C. F. (1988) Biochemistry 27, 8762-8767). In the present study, evidence has been obtained from electron microscopy for the formation of highly ordered and distinct lattices for two bivalent complex type oligosaccharides cross-linked with soybean lectin (Glycine max) and isolectin A from Lotus tetragonolobus, respectively. The results indicate a new source of specificity for interactions of N-linked carbohydrates with lectins, namely their ability to form highly ordered homogeneous aggregates.
Bhattacharyya
L
L
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461.
Khan
M I
MI
Fant
J
J
Brewer
C F
CF
D001216:Asparagine / Q000378:metabolism*; D003432:Cross-Linking Reagents; D006386:Hemagglutination Tests; D037102:Lectins / Q000378:metabolism*; D008854:Microscopy, Electron; D009844:Oligosaccharides / Q000378:metabolism*; D037121:Plant Lectins; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D001216:Asparagine; D003432:Cross-Linking Reagents; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins