title: Analysis of human metaphase chromosomes using antibodies to double-stranded and single-stranded DNA: staining patterns are related to DNA conformation.
abstract: Fresh and 6-day-old fixed chromosome spreads, both untreated and treated with various banding techniques and nucleases, were stained using monoclonal antibodies to double-stranded and single-stranded DNA. DNA in fixed chromosome preparations became progressively denatured with ageing. The staining pattern of untreated chromosomes with anti-double-stranded DNA antibodies (which resembles G-banding) was determined by the conformation of the chromosomal DNA.
authors.affiliation: Istituto di Biologia Generale, Università di Cagliari, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Mezzanotte
authors.forename: D
authors.initials: D
authors.lastname: Peretti
authors.forename: M G
authors.initials: MG
authors.lastname: Ennas
authors.forename: R
authors.initials: R
authors.lastname: Vanni
authors.forename: A T
authors.initials: AT
authors.lastname: Sumner
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis*; D002877:Chromosomes, Human / Q000648:ultrastructure*; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D009690:Nucleic Acid Conformation
chemical_list: D000911:Antibodies, Monoclonal; D004277:DNA, Single-Stranded; D004247:DNA