Intracellular localization of the calcium- and calmodulin antagonist fendiline.
Microautoradiograpic studies of mouse heart sections were performed to evaluate intracellular localization of the antianginal drug fendiline (Sensit). 15 microCi 3H-fendiline/g b. w. (0.5 mg/kg b.w.) were injected intravenously. 10 min p.a. the heart was removed and either frozen in acetone/dry ice or, after perfusion in situ with dextran 40 and formaldehyde, fixed in formaldehyde. Frozen, paraffin-embedded, and semithin epoxy resin sections were prepared and coated with Ilford K2 emulsion. After 7 to 30 days exposure and development in amidol silver grains were counted above cells, nuclei and extracellular space. The results show that fendiline is able to enter myocardial cells.
Weyhenmeyer
R
R
Abteilung Pharmakokinetik der Thiemann Arzneimittel GmbH, Waltrop Fed. Rep. of Germany.
Gross
M
M
Maurer-Schultze
B
B
D000818:Animals; D001345:Autoradiography; D002121:Calcium Channel Blockers / Q000378:metabolism*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D005260:Female; D005275:Fendiline / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010627:Phenethylamines / Q000378:metabolism*
D002121:Calcium Channel Blockers; D002147:Calmodulin; D010627:Phenethylamines; D005275:Fendiline