Mass spectral investigations on trichothecene mycotoxins. VIII--Thermospray ionization and collisionally activated dissociation of macrocyclic trichothecenes.
Several structurally similar, biologically active macrocyclic trichothecenes were ionized under thermospray conditions followed by collisionally activated dissociation of the ammonium adducts. Most of the observed daughter ions were formed by bond cleavage at the exocyclic ester bridges. Compounds with similar ester bridges formed several common daughter ions and underwent similar neutral losses. Fragmentation pathways proposed for the dissociation of the adducts were confirmed from the corresponding neutral loss spectra. Simple experiments designed for the sequential monitoring of the characteristic daughter ions were used for the rapid, direct and accurate analysis of macrocyclic trichothecenes in real samples. The primary drawback of the method is its inability to distinguish between isomers.
Krishnamurthy
T
T
US Army Chemical Research, Development and Engineering Center, Aberdeen Proving Ground, Maryland 21010.
Beck
D J
DJ
Isensee
R K
RK
D055598:Chemical Phenomena; D002621:Chemistry; D013058:Mass Spectrometry; D012717:Sesquiterpenes / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*
D012717:Sesquiterpenes; D014255:Trichothecenes