Chemiluminescence immunoassay for the detection and quantification of methyltestosterone residues in muscle tissue.
Chemiluminescence as a detection method for immunoassay has successfully been applied to the measurement of methyltestosterone (MT) residues in muscle tissue. The sample is digested enzymatically, extracted with diethyl ether and purified on a Lipidex-5000 column. An optional clean-up utilized disposable C18 columns. As the luminescent label the N-(4-aminobutyl)-N-ethylisoluminol conjugate of MT was used. The antiserum was raised in a rabbit against MT-3-carboxymethyloxime-bovine serum albumin. The detection limit of the assay was 14 +/- 7 pg (n = 13), with a limit of quantification in muscle tissue of 0.125 ppb.
van Peteghem
C
C
Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, State University of Ghent, Belgium.
van Look
L
L
De Guesquière
A
A
D000818:Animals; D002417:Cattle; D007118:Immunoassay; D008163:Luminescent Measurements; D008777:Methyltestosterone / Q000032:analysis*; D009132:Muscles / Q000032:analysis*
D008777:Methyltestosterone