Ultrastructural analysis of megakaryoblasts by simultaneous detection of platelet peroxidase and platelet glycoproteins.
We studied the phenotype of megakaryoblasts (MKB) in two patients with blastic crisis of chronic myeloid leukemia by simultaneous detection of platelet peroxidase (PPO) activity and platelet glycoproteins (GP) with monoclonal antibodies at the ultrastructural level. When 227A (anti-GPIb) was used, the finding of GP correlated well with the presence of PPO in the majority of MKB, and a few MKB were found to express PPO but lack GP. When 224B (anti-GPIIb/IIIa) was applied, we found a few MKB of a further type, which expressed GP but lacked PPO. These results indicate that the expression of platelet markers is partially aberrant in neoplastic MKB, and that MKB are heterogenous in their phenotypic expressions. Thus, the detection of PPO or GP alone may miss some MKB. For the accurate identification of MKB and the demonstration of aberrant expression of platelet markers, simultaneous detection of the markers seems to useful.
Kishimoto
Y
Y
Nagano
T
T
Kimura
T
T
Adachi
M
M
Tatsumi
E
E
Ryo
R
R
Yamaguchi
N
N
Yasunaga
K
K
D001752:Blast Crisis; D001792:Blood Platelets / Q000032:analysis* / Q000201:enzymology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000473:pathology; D008297:Male; D008533:Megakaryocytes / Q000032:analysis*; D008562:Membrane Glycoproteins / Q000097:blood*; D008854:Microscopy, Electron; D008875:Middle Aged; D010544:Peroxidases / Q000097:blood*
D008562:Membrane Glycoproteins; D010544:Peroxidases