The cytotoxicity of T-2 toxin and related 12,13-epoxytrichothecenes to Adriamycin-sensitive and -resistant P388 leukemia cells.
The cytotoxicity of T-2 toxin and related trichothecenes was studied in Adriamycin-sensitive and -resistant P388 leukemia cells in vitro. The structure-activity relationship indicated that a free hydroxyl in the C-3 position contributed to the activity. Free hydroxyls at the 4, 8, and 15 positions interfered with the activity, and their estrification resulted in improved cytotoxicity. The cytotoxic activity of these trichothecenes did not seem to be related to their degree of lipophilicity. Adriamycin-resistant P388 cells were cross-resistant to the trichothecenes, and this resistance could be circumvented by verapamil.
Ramu
A
A
Department of Radiation and Clinical Oncology, Hadassah University Hospital, Jerusalem, Israel.
Yagen
B
B
Ramu
N
N
D000818:Animals; D000970:Antineoplastic Agents; D002455:Cell Division / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D004354:Drug Screening Assays, Antitumor; D004951:Esterification; D006900:Hydroxylation; D007941:Leukemia P388 / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D012717:Sesquiterpenes / Q000494:pharmacology*; D012995:Solubility; D013329:Structure-Activity Relationship; D013605:T-2 Toxin / Q000494:pharmacology*; D014255:Trichothecenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology; D014700:Verapamil / Q000494:pharmacology; D014867:Water
D000970:Antineoplastic Agents; D012717:Sesquiterpenes; D014255:Trichothecenes; D014867:Water; D004317:Doxorubicin; D014700:Verapamil; D013605:T-2 Toxin