title: Evaluation of different plating media used in the isolation of salmonellas from environmental samples.
abstract: Different serotypes of salmonellas were compared for selectivity and efficiency of recovery using 11 plating media. No optimal growth was obtained after 24 h incubation in any of the media, but after 48 h, brilliant green, brilliant green-phenol red-lactose-sucrose, bismuth sulphite, xylose-lysine-deoxycholate and Hektoen enteric agars showed optimal recovery of all the salmonella serotypes. Xylose-lysine-deoxycholate and brilliant green-phenol red-lactose-sucrose agars were the most selective media for all salmonella serotypes. Addition of 10 micrograms/ml of sodium novobiocin to the tryptic soy-xylose-lysine and tryptic soy-brilliant green agars significantly improved their selectivity but reduced or inhibited the growth of some salmonella serotypes, including Salmonella typhi. Xylose-lysine-deoxycholate agar gave the highest recovery percentage of stressed salmonellas with a double-agar layer technique. Good recovery was also obtained on brilliant green-phenol red-lactose-sucrose, tryptic soy-brilliant green, tryptic soy-brilliant green-novobiocin, tryptic, soy-xylose-lysine and tryptic soy-xylose-lysine-novobiocin agars. Salmonella-shigella agar was the least efficient medium for the recovery of salmonellas under stress-induced or non-stressed conditions.
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Málaga, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Moriñigo
authors.forename: E
authors.initials: E
authors.lastname: Martinez-Manzanares
authors.forename: A
authors.initials: A
authors.lastname: Muñoz
authors.forename: R
authors.initials: R
authors.lastname: Cornax
authors.forename: P
authors.initials: P
authors.lastname: Romero
authors.forename: J J
authors.initials: JJ
authors.lastname: Borrego
mesh_terms: D003470:Culture Media; D012475:Salmonella / Q000302:isolation & purification*; D014871:Water Microbiology
chemical_list: D003470:Culture Media