title: Dual action of anti-sporozoite antibodies in vitro.
abstract: With the use of a double staining technique that permits localization of the sporozoite during the process of entering a host cell, we studied the biologic effects of three mAb directed against determinants contained in the circumsporozoite of Plasmodium yoelii. These mAb, which included one IgM and two IgG3, were studied in primary cultures of rodent hepatocytes inoculated with sporozoites of P. yoelii. These results confirm previous reports of the extended action of antibodies on Plasmodium falciparum after entering hepatocytes by producing a strong intrahepatocyte inhibitory effect in addition to the inhibitory effect on sporozoite entry. As with P. falciparum the intracellular effects on P. yoelii liver stages are only observed when the antibodies are present at the time the sporozoite enters the cell. While carrying out experiments on this phenomenon, it was discovered that, at lowered antibody concentrations, an increase in number of maturing liver schizonts occurs, with the increase or enhancement of infection reaching up to 150% of that of controls. It was also observed that there was an inverse relationship between the antibody concentration that was inhibitory and that which enhanced parasite infectivity.
authors.affiliation: INSERM U 313, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Nudelman
authors.forename: L
authors.initials: L
authors.lastname: Rénia
authors.forename: Y
authors.initials: Y
authors.lastname: Charoenvit
authors.forename: L
authors.initials: L
authors.lastname: Yuan
authors.forename: F
authors.initials: F
authors.lastname: Miltgen
authors.forename: R L
authors.initials: RL
authors.lastname: Beaudoin
authors.forename: D
authors.initials: D
authors.lastname: Mazier
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000913:Antibodies, Protozoan / Q000502:physiology*; D000953:Antigens, Protozoan / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D000962:Antimalarials / Q000502:physiology; D002448:Cell Adhesion; D002478:Cells, Cultured; D004705:Endocytosis; D004912:Erythrocytes / Q000469:parasitology; D007140:Immunoglobulin Fab Fragments / Q000502:physiology; D008099:Liver / Q000276:immunology / Q000469:parasitology*; D010967:Plasmodium yoelii / Q000254:growth & development / Q000276:immunology*; D015800:Protozoan Proteins; D051381:Rats; D012377:Rodentia / Q000469:parasitology; D013045:Species Specificity; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D000962:Antimalarials; D007140:Immunoglobulin Fab Fragments; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan