Role of cholesterol in the stability of pH-sensitive, large unilamellar liposomes prepared by the detergent-dialysis method.
Large unilamellar liposomes prepared by an octyl glucoside-dialysis method were examined for stability at 37 degrees C in the presence or absence of human plasma, using the release of the entrapped calcein as a fluorescence marker. The liposomes were acid-sensitive as they were composed of dioleoylphosphatidylethanolamine, oleic acid and cholesterol. The stability of the liposomes in the absence of plasma was significantly enhanced with increasing cholesterol content. However, the maximal calcein release at pH 5 decreased linearly with increasing cholesterol content of the liposome, indicating that cholesterol had reduced the acid-sensitivity of the liposomes. In the presence of human plasma, calcein release exhibited a biphasic behavior with a fast (plasma-sensitive) and a slow (plasma-resistant) component. Inclusion of cholesterol in the liposomes resulted in an increased proportion of the plasma-resistant component. Liposomes pretreated with human plasma, after removal of excess plasma and the released calcein by gel-filtration, showed a remarkable stability both in the presence and absence of human plasma. The acid-sensitivity of the plasma-treated liposomes with 40% cholesterol was the same as that of the untreated liposomes. These results are discussed in terms of the mechanism by which these liposomes deliver their contents to the cytoplasm of the cells via the endocytic pathway, a known biological activity of the type of liposome described here.
Liu
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Department of Biochemistry, University of Tennessee, Knoxville.
Huang
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L
D001769:Blood; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002784:Cholesterol; D003902:Detergents; D003956:Dialysis; D005452:Fluoresceins; D005960:Glucosides; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008081:Liposomes; D019301:Oleic Acid; D009829:Oleic Acids; D010714:Phosphatidylethanolamines
D003902:Detergents; D005452:Fluoresceins; D005960:Glucosides; D008081:Liposomes; D009829:Oleic Acids; D010714:Phosphatidylethanolamines; C018619:octyl-beta-D-glucoside; D019301:Oleic Acid; C020888:1,2-dielaidoylphosphatidylethanolamine; D002784:Cholesterol; C007740:fluorexon