Fatty acid alters glycogen metabolism in contracting vascular smooth muscle.
The purpose of this investigation was to study the effect of fatty acid on carbohydrate metabolism in contracting vascular smooth muscle. The glycogen content of porcine carotid artery incubated in media containing only glucose decreased markedly upon contraction with 80 mM KCl. In contrast, when 0.5 mM palmitate was included there was no decrease in glycogen. Furthermore, the maximal isometric force generated was 22% greater than in the absence of palmitate (P less than 0.001). Stimulation of glycogen phosphorylase a activity with contraction was also enhanced with palmitate. Palmitate had no effect on the levels of the high energy phosphates under any substrate condition. Thus, fatty acid profoundly affects glycogen metabolism and contractility in vascular smooth muscle.
Barron
J T
JT
Department of Medicine, Rush Medical College, Rush-Presbyterian St. Luke's Medical Center, Chicago, IL 60612.
Kopp
S J
SJ
D000818:Animals; D002339:Carotid Arteries; D006003:Glycogen / Q000378:metabolism*; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology*; D010762:Phosphorylase a / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D013552:Swine
D007773:Lactates; D010169:Palmitic Acids; D019308:Palmitic Acid; D019344:Lactic Acid; D006003:Glycogen; D010762:Phosphorylase a; D011188:Potassium