title: Bovine tryptophanyl-tRNA synthetase and glyceraldehyde-3-phosphate dehydrogenase form a complex.
abstract: Bovine tryptophanyl-tRNA synthetase is able to form a complex with glyceraldehyde-3-phosphate dehydrogenase. The complex formation (i) does not influence the tryptophan-dependent PPi-ATP exchange reaction and (ii) involves predominantly the N-terminal dispensable domain of the synthetase. Glyceraldehyde-3-phosphate dehydrogenase was shown to be capable of interacting simultaneously with tryptophanyl-tRNA synthetase and with ribosomal RNA to form a ternary complex. It is proposed that compartmentation of some aminoacyl-tRNA synthetases in certain cases might be achieved via 'adapter' molecules which can bind at once to ribonucleic acids and to aminoacyl-tRNA synthetases.
authors.affiliation: Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
authors.forename: V V
authors.initials: VV
authors.lastname: Filonenko
authors.forename: S F
authors.initials: SF
authors.lastname: Beresten
authors.forename: B I
authors.initials: BI
authors.lastname: Rubikaite
authors.forename: L L
authors.initials: LL
authors.lastname: Kisselev
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002451:Cell Compartmentation; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D007700:Kinetics; D009097:Multienzyme Complexes; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000378:metabolism; D014369:Tryptophan-tRNA Ligase / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D012335:RNA, Ribosomal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase