The effect of dexamethasone on chronic pulmonary oxygen toxicity in infant mice.
The effect of dexamethasone (0.1, 1, and 5 mg/kg/d given subcutaneously from d 14-18) was tested in infant mice continuously exposed from birth to either humidified air or 80% oxygen. Dexamethasone significantly decreased lung wet wt (p less than 0.01), lung water (p less than 0.021), lung dry wt, protein, and DNA (p less than 0.001) in both air- and oxygen-exposed animals. Dexamethasone, however, had no effect on lung compliance measured after animals were killed on d 18. It also had no effect on the increase in the blood-air barrier thickness or decrease in the blood-air exchange surface area seen in the 80% oxygen-exposed mice. Dexamethasone decreased thymus gland wt (p less than 0.001), body wt gain (p less than 0.001), brain wt (p less than 0.001), and lung lymphocytes (p less than 0.05) in both air- and oxygen-exposed animals. The effect of 1 mg/kg and 5 mg/kg of the drug could not be differentiated. During the 4 d of drug administration, one air- and one oxygen-exposed animal died; both received 5 mg/kg/d of dexamethasone; microscopic and culture evidence of infection was not found. If dexamethasone causes similar effects in human infants with bronchopulmonary dysplasia, it should be used with great caution even for short-term clinical management.
Ohtsu
N
N
Department of Radiology, Stanford University Medical Center, California 94305.
Ariagno
R L
RL
Sweeney
T E
TE
Davis
L
L
Moses
L
L
Petriceks
R
R
Daehne
I
I
Bensch
K
K
Northway
W H
WH
D000818:Animals; D000831:Animals, Newborn; D001997:Bronchopulmonary Dysplasia / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D002908:Chronic Disease; D003094:Collagen / Q000032:analysis; D004247:DNA / Q000032:analysis; D003907:Dexamethasone / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D007231:Infant, Newborn; D008168:Lung / Q000032:analysis / Q000473:pathology; D051379:Mice; D010100:Oxygen / Q000037:antagonists & inhibitors / Q000633:toxicity; D011506:Proteins / Q000032:analysis
D011506:Proteins; D003907:Dexamethasone; D003094:Collagen; D004247:DNA; D010100:Oxygen