A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes.
A new simplified radioassay for measuring polymorphonuclear leukocyte (PMN) chemotaxis is proposed using 51Cr-labeled cells and a single-filter system. The technique offers all the advantages described for the double-filter radioassay and permits a reproducible measurement of random locomotion, chemokinesis and chemotaxis. Moreover the single-filter radioassay utilizes commercially available and disposable chambers gathered in a multichamber apparatus; this makes the method very easy to learn and rapid to perform.
Capsoni
F
F
Istituto di Clinica Medica I, University of Milan, Ospedale Policlinico, Italy.
Minonzio
F
F
Ongari
A M
AM
Zanussi
C
C
D002465:Cell Movement; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002860:Chromium Radioisotopes; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D013997:Time Factors
D002860:Chromium Radioisotopes; D009240:N-Formylmethionine Leucyl-Phenylalanine