title: Isolated soluble fractions from spleen cell culture supernatants induce tumor enhancement.
abstract: Supernatants of spleen cell culture from small-tumor-bearing mice, large-tumor-bearing mice, and large-tumor resected mice were fractionated by Sephadex G-100. The biological activity on tumor growth of all fractions was tested in vivo. It was found that only one fraction (MW: 220-250 kD) from spleen cell supernatants of small-tumor-bearing mice or large-tumor resected mice enhanced tumor growth. In spleen cell supernatants from large-tumor-bearing mice, two fractions had enhancing activity (MW: 220-250 and 100-10 kD). Thus, the surgical resection of large tumor induced disappearance of enhancing activity from the fraction of lower molecular weight.
authors.affiliation: Research Department, Institute of Oncology, A. H. Roffo, Buenois Aires, Argentina.
authors.forename: A M
authors.initials: AM
authors.lastname: Eiján
authors.forename: M A
authors.initials: MA
authors.lastname: Jasnis
authors.forename: A B
authors.initials: AB
authors.lastname: Motta
authors.forename: S
authors.initials: S
authors.lastname: Oisgold-Dagá
mesh_terms: D000230:Adenocarcinoma / Q000032:analysis*; D000818:Animals; D007108:Immune Tolerance; D008325:Mammary Neoplasms, Experimental / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000032:analysis*; D014407:Tumor Cells, Cultured