Histone electrophoretic pattern in the characterization of synaptonemal complexes.
The presence of a stoechiometric electrophoresis pattern of histones in carefully isolated synaptonemal complexes is reported. The use of this pattern is suggested as an internal standard of synaptonemal complex purification, in addition to the more generally used electron micrographs. This is especially useful in experiments leading to the characterization of the protein components of SCs. The use of mice of an age at which pachytenes predominate (90%) in the prophase-cell population is also advantageous to improve the final yield in synaptonemal complexes.
Vilardell
J
J
Coll
M D
MD
Querol
E
E
Egozcue
J
J
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification*; D008297:Male; D008540:Meiosis; D051379:Mice; D013090:Spermatocytes / Q000032:analysis / Q000648:ultrastructure; D013573:Synaptonemal Complex
D006657:Histones