title: Semen analysis data from fresh and cryopreserved donor ejaculates: comparison of cryoprotectants and pregnancy rates.
abstract: Patients (155) were selected at random for fresh or cryopreserved semen and inseminated on the predicted day of ovulation. Semen analysis was performed using a microcomputerized multiple-exposure photography system. Frozen semen was used with either glycerol or TEST-yolk (TEST-buffered 20% egg yolk with 10% glycerol) as the cryoprotectant. Cryopreservation resulted in significant decreases in all semen parameters measured. Of these, velocity appeared to be the least effected. TEST-yolk provided significantly more protection against a reduction in velocity compared with glycerol. A total of 18, 17, and 27 patients conceived using fresh, glycerol, or TEST-yolk-preserved semen, respectively. For these same groups, a cumulative pregnancy rate of 52.9%, 27.1%, and 68.5%, respectively, was observed (not significant). The total number of motile sperm per insemination used for fresh artificial inseminations resulting in conception (132.4 X 10(6] was significantly greater than the number used for successful glycerol- and TEST-yolk-preserved semen (approximately 24 X 10(6]. These results demonstrate that although the number of motile sperm of cryopreserved ejaculates are dramatically reduced compared with the fresh counterparts, if a minimum criteria for ejaculate quality is established, the use of cryopreserved semen can offer a viable, effective, and relatively safe alternative to artificial insemination by donor with fresh semen.
authors.affiliation: Department of Obstetrics and Gynecology, University of Kansas School of Medicine, Wichita.
authors.forename: B A
authors.initials: BA
authors.lastname: Keel
authors.forename: B W
authors.initials: BW
authors.lastname: Webster
mesh_terms: D002470:Cell Survival; D003451:Cryoprotective Agents; D005260:Female; D005298:Fertility; D005615:Freezing; D006801:Humans; D007315:Insemination, Artificial / Q000379:methods*; D007316:Insemination, Artificial, Heterologous / Q000379:methods*; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000166:cytology*; D012662:Semen Preservation; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology
chemical_list: D003451:Cryoprotective Agents