Study and development of multilayer needle-type enzyme-based glucose microsensors.
Glucose oxidase (GOD) was covalently coupled to a cellulose acetate (CA) layer, using bovine serum albumin (BSA) and parabenzoquinone (PBQ) linkages. Prior to GOD coupling this CA layer was deposited on the platinum tip of a needle-type sensor and covered with an outer layer of polyurethane (PU). Such microsensors were found to be active, their GOD load reaching 1.6 to 3.0 micrograms mm-2 and their glucose response reaching 1 to 3 microA M-1 mm-2, even when the upper limit of their linear range reached 10-30 mM. Due to the multilayer structure and composition of these microsensors, small anions such as ascorbate were partially discriminated from neutral molecules such as hydrogen peroxide. When implanted subcutaneously in anaesthetized rats, sensor responses correlated correctly with blood glucose concentration but presented sensitivity coefficients significantly different to those determined in vitro: a 2 point calibration procedure was found necessary for in vivo experiments.
Sternberg
R
R
Laboratoire de Bioélectrochimie et Analyse du Milieu, U.F.R. de Sciences et de Technologie, Université Paris-Val de Marne, Créteil, France.
Barrau
M B
MB
Gangiotti
L
L
Thévenot
D R
DR
Bindra
D S
DS
Wilson
G S
GS
Velho
G
G
Froguel
P
P
Reach
G
G
D000818:Animals; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis; D002482:Cellulose / Q000031:analogs & derivatives; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D008297:Male; D010539:Permeability; D011140:Polyurethanes; D019736:Prostheses and Implants; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D011140:Polyurethanes; C005062:acetylcellulose; D002482:Cellulose; D005949:Glucose Oxidase; D005947:Glucose