[Dynamics of amino acid and protein metabolism in laying hens after the administration of 15N-labeled wheat protein. 11. Incorporation of 15N in the tissues and the amino acids of the muscles].
Over a period of 4 days 12 colostomized laying hens daily received 36 g 15N labelled wheat with 15N excess (15N') of 14.37 atom-% together with a conventional feed mixture for laying hens. The labelling of the lysine N in the wheat was 13.58 atom-%, that of histidine N 14.38 and that of arginine 15N' 13.63 atom-% 15N'. Three hens each were butchered 12, 36, 60 and 108 h after the last 15N' feeding. The first three hens did not receive any feed before being butchered. The following three hens each received the unlabelled feed ration for another 1, 2 or 4 days resp. after the main period until they were butchered. The total of skeleton muscles, the heart and the stomach muscle (without inner skin) of each hen were combined into one sample, cut thinly, drenched with fluid nitrogen and pulverized. N, 15N' and the basic and non-basic amino acids as well as their 15N' were determined in the individual samples. In contrast to the organs, the proteins in the muscle tissue have a long half life so that a slight decrease of atom-% 15N' in the muscles could only be detected after 108 h. The 14N and 15N' quota of the non-basic amino acids in the total nitrogen of the muscles is 50%. The 14N quota of the basic amino acids is 30% and the 15N' quota only 22.5% in the total muscle N. The heavy nitrogen of the free lysine in the TCA soluble N fraction is hardly detectable 36 h and 60 h after the last 15N' supply and not at all after 108 h. In contrast to this, the other two free basic amino acids remain significantly higher labelled in dependence on the last butchering time.
Gruhn
K
K
Sektion Tierproduktion und Veterinärmedizin, Karl-Marx-Universität Leipzig.
Zander
R
R
D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D004044:Dietary Proteins / Q000008:administration & dosage; D005260:Female; D009132:Muscles / Q000032:analysis*; D009206:Myocardium / Q000032:analysis; D009587:Nitrogen Isotopes; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D013270:Stomach; D014908:Triticum
D000596:Amino Acids; D004044:Dietary Proteins; D009587:Nitrogen Isotopes; D011506:Proteins
Dynamik des Aminosäuren- und Proteinstoffwechsels der Legehennen nach Applikation von 15N-markiertem Weizenprotein. 11. Mitteilung. Inkorporation des 15N in das Gewebe und die Aminosäuren der Muskulatur.