Effect of cerulein on in vivo release of acetylcholine from the rat striatum.
An effect of cerulein on in vivo release of acetylcholine (ACh) from the rat striatum was examined by means of intracerebral dialysis. Intraperitoneal administration of cerulein (25-200 micrograms/kg) enhanced a spontaneous release of ACh in a dose-dependent manner. Intraperitoneal administration of cerulein (100 micrograms/kg) also enhanced the K+-evoked (30 mM) release of ACh. Bilateral subdiaphragmatic vagotomy reduced the increase of both K+-evoked and that of spontaneous release of ACh induced by cerulein administration. Pretreatment with haloperidol (0.5 mg/kg, i.p.) had no effect on increase in spontaneous release of ACh brought about by cerulein administration (100 micrograms/kg, i.p.). These results suggested that peripherally administered cerulein stimulated striatal cholinergic neurons, that its stimulatory effect on striatal ACh release was not mediated by striatal dopamine D2 receptors and that the action of cerulein was, in part, mediated via vagal afferent impulses.
Shimoyama
M
M
Third Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Kito
S
S
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002108:Ceruletide / Q000494:pharmacology*; D002799:Cholinergic Fibers / Q000187:drug effects / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014630:Vagus Nerve / Q000502:physiology
D002108:Ceruletide; D006220:Haloperidol; D000109:Acetylcholine; D011188:Potassium