Identification of drug receptors in porcine dental pulp by the radioligand binding assay.
1. The acetylcholine (ACh), histamine and serotonin (5-HT) receptors in porcine dental pulp were characterized by the radioligand binding assay. 2. For [3H]nicotine binding site, Kd was 8.06 +/- 1.65 nM and Bmax was 270.83 +/- 32.68 fmol/mg protein. 3. For [3H]QNB binding site, Kd was 1.04 +/- 0.14 nM and Bmax was 24.83 +/- 3.09 fmol/mg protein. 4. For [3H]histamine binding site, Kd was 1.22 +/- 0.1 nM and Bmax was 283.15 +/- 33.1 fmol/mg protein. 5. For [3H]5-HT binding site, Kd was 1.41 +/- 0.1 nM and Bmax was 53.1 +/- 3.4 fmol/mg protein. 6. These findings indicate that the specific receptors for ACh, histamine and 5-HT are present in the porcine dental pulp, and that the ACh receptor is predominantly nicotinic.
Sano
M
M
Department of Oral Physiology, School of Dentistry, Showa University, Tokyo, Japan.
Murakami
H
H
Tsukimura
T
T
Yamazaki
A
A
D000818:Animals; D003782:Dental Pulp / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006632:Histamine / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009538:Nicotine / Q000378:metabolism; D011813:Quinuclidinyl Benzilate; D011869:Radioligand Assay; D011955:Receptors, Drug / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D013552:Swine; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
D009419:Nerve Tissue Proteins; D011955:Receptors, Drug; D012701:Serotonin; D011813:Quinuclidinyl Benzilate; D009538:Nicotine; D006632:Histamine