title: The role of charge neutralization and cooperative binding of linker histone in the higher-order structure of chromatin.
abstract: The binding mode and stoichiometry of interaction between soluble rat liver chromatin and histone H1 (H1) were studied. H1 binding to chromatin is cooperative. Chromatin accepts 3.6 molecules of H1/nucleosome at 0 M salt, close to the required ratio for neutralization of 90% of the charges on the phosphate groups of chromatin (4.0 H1 molecules/nucleosome). The proposal is put forward that critical charge neutralization (90%) has a significant influence on the irregular appearance of chromatin.
authors.affiliation: Institut für Zellbiologie, ETH Hönggerberg, Zürich, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001665:Binding Sites; D002843:Chromatin / Q000378:metabolism*; D004563:Electrochemistry; D005453:Fluorescence; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism; D051381:Rats
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes