Isolation and characterization of an ipt gene from the Ti plasmid Bo542.
A 1.9 kb clone of the T-DNA region of the Agrobacterium tumefaciens Ti plasmid Bo542 which exhibited homology to the isopentenyl transferase (ipt) locus of pTiA6 was identified by low stringency DNA hybridization. Introduction of this segment of pTiBo542 DNA into cells of Nicotiana tabacum or N. glauca caused tumor formation in vivo, and allowed hormone independent growth in vitro. Furthermore, this DNA segment complemented ipt mutant strains of A. tumefaciens, restoring their ability to cause tumors on Kalanchöe leaves and tomato stems. The complete DNA sequence of this segment has been determined, revealing an open reading frame homologous to other known Agrobacterium ipt genes.
Strabala
T J
TJ
Department of Biochemistry, University of Wisconsin-Madison 53706.
Bednarek
S Y
SY
Bertoni
G
G
Amasino
R M
RM
D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010941:Plant Tumors / Q000382:microbiology; D010957:Plasmids; D012231:Rhizobium / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014166:Transferases / Q000235:genetics
D004269:DNA, Bacterial; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase