[A panel of monoclonal antibodies against human lymphocyte antigens].
Monoclonal antibodies (McAb) LT1, LT2, LT4, LT6, LT7, LT8, LB21 and LDR to surface antigens of human lymphocytes have been obtained. Basing on the data concerning the distribution of McAb reactivity with cells from different organs and tissues, and with tumour cell lines, on experiments on the mutual blockage and comodulation of antigens, on two-colour immunofluorescence and determination of the molecular weight of the detected antigens a conclusion is made that the McAb obtained react with CD5, CD2, CD4, CD6, CD7, CD8, CD21 and HLA-DR antigens, respectively. McAb LT1 and Leu1, as well as LT2 and Leu5b in pairs blocked each other completely. The determinant recognized by McAb LT8 is more completely overlapped with the epitope, reacting with McAb OKT8. The McAb LT4 binding site is only partially overlapped with the McAb Leu3 site of recognition and practically is not bound to the epitope recognized by McAb OKT4.
Filatov
A V
AV
Bachurin
P S
PS
Markova
N A
NA
Surnakova
N E
NE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D000941:Antigens / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011233:Precipitin Tests; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000941:Antigens; D000954:Antigens, Surface
Panel' monoklonal'nykh antitel protiv antigenov limfotsitov cheloveka.