[A method of shaking-blotting--a simple and reliable means for obtaining direct chromosomal preparations from chorionic biopsies].
The method proposed is based on a gradual fixation, and short-term hydration before softening and on a new technique of chromosome preparation. The latter is based on the sample softening in 60% acetic acid directly on the slide, its gentle shaking and spot-blotting, a careful spreading of the cell suspension on slide surface avoiding the usage of micropipets and other handlings causing metaphase plate breakage. The method provides a highly efficient karyotyping of human embryos within the 7-12th weeks of gestation.
Baranov
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VS
D002823:Chorion / Q000648:ultrastructure*; D015193:Chorionic Villi Sampling / Q000379:methods*; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005260:Female; D005865:Gestational Age; D006801:Humans; D007621:Karyotyping / Q000379:methods; D011247:Pregnancy
Metod striakhivaniia--otpechatyvaniia--prostoĭ i nadezhnyĭ sposob polucheniia priamykh khromosomnykh preparatov iz bioptatov khoriona.