[Immunohistochemical detection of triiodothyronine in thyroid tumors using T3-01 monoclonal antibody].
Cells of the spleen of BALB/c mice immunized with a conjugate of triiodothyronine and bovine immunoglobulin were fused with cells of the murine myeloma line F O. Selection and cloning were used to look up the T3-01 hybridoma producing a monoclonal antibody of the IgG1 subclass which responded in a radioimmunoassay specifically to triiodothyronine. The T3-01 monoclonal antibody was used for the immunohistochemical demonstration of triiodothyronine on 19 tumour derivatives.
Michal
M
M
Franĕk
F
F
Nedvídková
J
J
Kovár
J
J
Felt
V
V
D000911:Antibodies, Monoclonal; D006801:Humans; D007150:Immunohistochemistry; D013964:Thyroid Neoplasms / Q000032:analysis*; D014284:Triiodothyronine / Q000032:analysis*
D000911:Antibodies, Monoclonal; D014284:Triiodothyronine
Imunohistochemický průkaz trijódtyroninu v nádorech stítné zlázy monoklonální protilátkou T3-01.