Comparison of Coulter volumes with radiometrically determined intracellular water volumes for cultured cells.
During methotrexate-induced differentiation of cultured human choriocarcinoma (BeWo) cells, proliferation is inhibited, morphologic and biochemical changes occur, and giant, often multinucleated, cells form. We have used the increase in cell volume as a marker of the mature syncytiotrophoblastlike phenotype. Uninduced and differentiated BeWo cells are not spherical, and theoretical considerations suggested that deviations in shape could result in significant errors in Coulter volume. To determine if the values obtained by electrical pulse sizing reflected the actual mass of BeWo cells, we have evaluated the relationship between Coulter volumes and intracellular water volumes obtained using a shape-independent estimate for eight cell types. A close correlation (r2 = 0.97) was found, indicating that cell volume changes in populations of irregularly shaped cells can be accurately measured using a Coulter instrument.
Burres
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McEachern Laboratory, University of Alberta, Edmonton, Canada.
Cass
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CE
D001826:Body Fluids / Q000032:analysis*; D001834:Body Water / Q000032:analysis*; D002452:Cell Count / Q000379:methods; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002822:Choriocarcinoma / Q000032:analysis / Q000473:pathology*; D004553:Electric Conductivity; D006801:Humans; D007424:Intracellular Fluid / Q000032:analysis*; D008727:Methotrexate / Q000494:pharmacology; D011874:Radiometry / Q000379:methods; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000187:drug effects / Q000473:pathology
D008727:Methotrexate