[The use of a tripeptide chromogenic substrate for simultaneous determination of 4 indices of kallikrein-kinin system].
Evaluation of the kallikrein-kinine system activity in blood plasma is of importance due to participation of the components of this system in regulation of blood plasma proteolysis. Using D-Pro-Phe-Arg-pNA as a substrate, a procedure for simultaneous estimation of four patterns of the kallikrein-kinine system activity was developed: I. maintenance of contact phase, 2. content of prekallikrein (1.49 un/ml in normal state), 3. activity of kallikrein (0.104 un/ml), 4. antikallikrein potential (0.700 IU/ml).
Dotsenko
V L
VL
Iarovaia
G A
GA
Neshkova
E A
EA
D000581:Amidohydrolases / Q000097:blood; D002863:Chromogenic Compounds; D006801:Humans; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000097:blood*; D007700:Kinetics; D007705:Kinins / Q000097:blood*; D009842:Oligopeptides; D011288:Prekallikrein / Q000302:isolation & purification; D011480:Protease Inhibitors
D002863:Chromogenic Compounds; D007705:Kinins; D009842:Oligopeptides; D011480:Protease Inhibitors; C026148:prolyl-phenylalanyl-arginine-4-nitroanilide; D011288:Prekallikrein; D007610:Kallikreins; D000581:Amidohydrolases; C019665:amidase
Ispol'zovanie tripeptidnogo khromogennogo substrata dlia odnovremennoĭ otsenki chetyrekh pokazateleĭ aktivnosti kallikrein-kininovoĭ sistemy.