Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.
A cDNA for threonyl-tRNA synthetase was isolated from a human placental cDNA lambda gt11 expression library by immunological screening, and its identity was confirmed by hybrid-selected mRNA translation. With this cDNA used as a hybridization probe, borrelidin-resistant Chinese hamster ovary cells that overproduced threonyl-tRNA synthetase were shown to have increased levels of threonyl-tRNA synthetase mRNA and gene sequences. Amplification of the gene did not appear to have been accompanied by any major structural reorganizations.
Kontis
K J
KJ
Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717.
Arfin
S M
SM
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D005233:Fatty Alcohols / Q000494:pharmacology; D005784:Gene Amplification; D005796:Genes; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D013914:Threonine-tRNA Ligase / Q000235:genetics*
D005233:Fatty Alcohols; D012333:RNA, Messenger; C005066:borrelidin; D004247:DNA; D000604:Amino Acyl-tRNA Synthetases; D013914:Threonine-tRNA Ligase