title: Specificity of the activation of [3H]hemicholinium-3 binding by phospholipase A2.
abstract: Phospholipase A2 (PLA2) treatment has been shown previously to stimulate the sodium-dependent high-affinity choline uptake system as assessed by both the specific binding of [3H]hemicholinium-3 ([ 3H]HCh-3) and the uptake of [3H]choline. In the present study, the specificity of PLA2-induced stimulation upon [3H]HCh-3 binding has been examined. PLA2, as well as phospholipase C (PLC), treatment of synaptic membranes produced a dose-dependent increase in the specific binding of [3H]HCh-3 whereas neither phospholipase B nor phospholipase D had any effect. PLC-induced stimulation of [3H]HCh-3 binding resulted from a significant decrease in the Kd without a change in the maximum binding of [3H]HCh-3 binding. PLC treatment of synaptosomes resulted in an inhibition of [3H]choline uptake accompanied by an inhibition of Na+, K+-adenosine triphosphatase activity. In contrast to the increase of [3H]HCh-3 binding, the specific binding of both [3H]desipramine and [3H]mazindol was decreased by PLA2 treatment. After PLA2 treatment, [3H]HCh-3 binding was increased about 2.5-fold over basal levels in different regions of the brain. Electrolytic lesions of the medial septal nucleus and kainic acid-induced lesions of the striatum resulted in a marked reduction of [3H]HCh-3 binding in the hippocampus and the striatum, respectively. Residual [3H]HCh-3 binding in the denervated hippocampus and lesioned striatum was increased by PLA2 treatment but remained lower than that in PLA2-treated controls. Finally, atropine-induced up-regulation of [3H]HCh-3 binding in vivo was not additive with PLA2-induced stimulation. These results support the hypothesis that PLA2 might be involved in the regulation of the sodium-dependent high-affinity choline uptake.
authors.affiliation: Department of Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: M D
authors.initials: MD
authors.lastname: Saltarelli
authors.forename: J T
authors.initials: JT
authors.lastname: Coyle
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D003891:Desipramine / Q000378:metabolism; D006426:Hemicholinium 3 / Q000378:metabolism*; D008297:Male; D008454:Mazindol / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013570:Synaptic Membranes / Q000201:enzymology / Q000378:metabolism; D014316:Tritium
chemical_list: D010743:Phospholipids; D014316:Tritium; D006426:Hemicholinium 3; D008454:Mazindol; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002794:Choline; D003891:Desipramine