Characterization of a new temperature-sensitive and avirulent mutant of the rabies virus.
A temperature-sensitive (ts) mutant, tsG1, has been isolated from the CVS (Challenge Virus Standard) strain of rabies virus. The ts mutation affects the glycoprotein (G protein); it consists of an amino acid substitution (leucine to phenylalanine) at position 132. tsG1 exhibits a slightly reduced pathogenicity when administered via the intracerebral route and complete avirulence after intramuscular inoculation, associated with a very high protective power for adult mice. The ts mutation does not seem to block the transport of the G protein to the plasma membrane at the non-permissive temperature (39.6 degrees C). It abolishes the c.p.e. of the virus in cell cultures.
Préhaud
C
C
Laboratoire de Génétique des Virus, CNRS, Gif sur Yvette, France.
Coulon
P
P
Diallo
A
A
Martinet-Edelist
C
C
Flamand
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D008562:Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010449:Peptide Mapping; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology; D013696:Temperature; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014774:Virulence
D004279:DNA, Viral; D008562:Membrane Glycoproteins; D014764:Viral Proteins