title: Cerebral ischemia alters cerebral microvascular reactivity in newborn pigs.
abstract: The effects of cerebral ischemia on cerebral microvascular reactivity and prostanoid synthesis were examined in chloralose-anesthetized newborn pigs. Microvascular responses and periarachnoid cerebrospinal fluid (CSF) prostanoid concentrations were determined between 10 and 140 min after a 20-min period of total cerebral ischemia, as well as in sham-control piglets without cerebral ischemia. After cerebral ischemia, the decrease in pial arteriolar diameter in response to topical norepinephrine (10(-4) M) was similar in sham (-27 +/- 6%) and postischemic (-25 +/- 5%) piglets. However, the increase in pial arteriolar diameter in response to hypercapnia (10% CO2 ventilation) that was observed in sham piglets (+21 +/- 5%) was absent after ischemia (-2 +/- 3%). In contrast, dilations of pial arterioles in response to topical prostaglandin (PG)E2 (at 100 ng PGE2/ml: sham, +13 +/- 3%; postischemia, +21 +/- 4%) and topical isoproterenol (10(-6) M) (sham, +29 +/- 4%; postischemia, +23 +/- 3%) were not decreased by prior cerebral ischemia. In sham piglets, norepinephrine and hypercapnia produced increases in cortical periarachnoid prostanoid concentrations, whereas after cerebral ischemia, neither stimulus increased cortical periarachnoid prostanoid concentrations. The results are consistent with the hypothesis that failure of hypercapnia to dilate pial arterioles after cerebral ischemia results from the inability of this stimulus to increase cerebral vasodilator prostanoid synthesis.
authors.affiliation: Department of Physiology and Biophysics, University of Tennessee, Memphis 38163.
authors.forename: C W
authors.initials: CW
authors.lastname: Leffler
authors.forename: D G
authors.initials: DG
authors.lastname: Beasley
authors.forename: D W
authors.initials: DW
authors.lastname: Busija
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001160:Arterioles / Q000503:physiopathology; D002545:Brain Ischemia / Q000503:physiopathology*; D002560:Cerebrovascular Circulation; D015232:Dinoprostone / Q000494:pharmacology; D006935:Hypercapnia / Q000503:physiopathology; D011453:Prostaglandins / Q000134:cerebrospinal fluid; D013552:Swine; D014664:Vasodilation
chemical_list: D011453:Prostaglandins; D015232:Dinoprostone