title: A novel metabolite in the microbial degradation of 2-chlorobenzoate.
abstract: A bacterium which utilizes 2-chlorobenzoate as sole source of carbon and energy was isolated from water and tentatively identified as a Pseudomonas sp. During growth on 2-chlorobenzoate, the chlorine substituent was released quantitatively and a metabolite accumulated in the culture medium. Based on thin-layer chromatography, high-pressure liquid chromatography and gas chromatography linked to mass spectrometry this compound was unequivocally identified as 2,3-dihydroxybenzoate, a compound not described before in the degradation of 2-chlorobenzoate.
authors.affiliation: Institut für Mikrobiologie der Universität Hohenheim, Stuttgart, FRG.
authors.forename: S
authors.initials: S
authors.lastname: Fetzner
authors.forename: R
authors.initials: R
authors.lastname: Müller
authors.forename: F
authors.initials: F
authors.lastname: Lingens
mesh_terms: D002723:Chlorobenzoates / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D062385:Hydroxybenzoates / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002723:Chlorobenzoates; D062385:Hydroxybenzoates; C009135:2,3-dihydroxybenzoic acid