title: Levels and size complexity of DNA polymerase beta mRNA in rat regenerating liver and other organs.
abstract: A cDNA probe encoding DNA polymerase beta (beta-pol) was used to study the level and size complexity of beta-pol mRNA in regenerating rat liver and other rat tissues. An almost 2-fold increase in beta-pol mRNA was observed 18-24 h after partial hepatectomy. In most adult rat tissues (liver, heart, kidney, stomach, spleen, thymus, lung and brain) the abundance of beta-pol mRNA was low. In contrast, young brain and testes exhibited beta-pol mRNA levels 5- and 15-times higher, respectively. The observed changes in the level of beta-pol mRNA in regenerating rat liver and in developing brain are correlated with reported changes in DNA polymerase beta activity. Four different (4.0, 2.5, 2.2, 1.4 kb) transcripts hybridizing to beta-pol probe were found in all tissues examined. The 4.0 kb transcript was dominant for young and adult brain, whereas the 1.4 kb transcript was dominant for testes. The significance of these transcripts is discussed.
authors.affiliation: Department of Cell Biology and Experimental Therapy, Marie Sklodowska-Curie Institute, Warsaw, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Nowak
authors.forename: J A
authors.initials: JA
authors.lastname: Siedlecki
authors.forename: L
authors.initials: L
authors.lastname: Kaczmarek
authors.forename: B Z
authors.initials: BZ
authors.lastname: Zmudzka
authors.forename: S H
authors.initials: SH
authors.lastname: Wilson
mesh_terms: D000818:Animals; D002453:Cell Cycle; D004256:DNA Polymerase I / Q000096:biosynthesis / Q000235:genetics*; D015342:DNA Probes; D004586:Electrophoresis; D006498:Hepatectomy; D015151:Immunoblotting; D008099:Liver / Q000201:enzymology*; D008115:Liver Regeneration; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D013997:Time Factors
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D004256:DNA Polymerase I