The isolation and identification of indigo and indirubin from urine of a patient with leukemia.
The urine of a patient who suffered from acute myelomonocytic leukemia was red coloured after administration of mitoxantrone and etoposid. The isolation and spectroscopic identification of the excreted pigments resulted in the chemical structures of indigo and indirubin. The structure elucidation has been carried out by taking use of a two-dimensional technique in high-resolution nuclear magnetic resonance spectroscopy and different mass spectrometric methods, including tandem mass spectrometry. Possible reasons for the formation of the indigoid compounds are discussed.
Blanz
J
J
Institut für organische Chemie, Universität Tübingen.
Ehninger
G
G
Zeller
K P
KP
D000903:Antibiotics, Antineoplastic / Q000652:urine*; D002852:Chromatography, Ion Exchange; D006801:Humans; D007203:Indigo Carmine; D007211:Indoles / Q000652:urine*; D007938:Leukemia / Q000652:urine*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
D000903:Antibiotics, Antineoplastic; D007211:Indoles; D007203:Indigo Carmine; C027185:indirubin