title: In situ distribution of enolase isozymes in chronic liver disease.
abstract: Liver biopsy specimens with or without chronic liver diseases were examined immunohistochemically to determine the distribution of enolase isozymes (alpha, beta, and gamma). In normal liver, alpha-enolase was positively stained in almost all hepatocytes and bile duct cells. beta- and gamma-enolases were localized in hepatocytes and bile duct cells, respectively. Electron microscopic studies revealed that Kupffer cells and sinusoidal endothelial cells had both alpha- and gamma-enolases. In chronic active hepatitis and cirrhosis, proliferated biliary ductular cells had both alpha- and gamma-enolases, but did not express beta-enolase. This is almost the same localization pattern of enolase isozymes as in preexisting bile duct cells. gamma-Enolase was detected in some hepatocytes in eight of 12 cases with chronic active hepatitis and six of 12 cases with cirrhosis. These hepatocytes were small, showed a cobblestone pattern, and binucleate cells were frequent. On the other hand, rosette-formed hepatocytes adjacent to a regenerating bulging lobule were not stained for gamma-enolase. These results suggest that regenerating hepatocytes have gamma-enolase and that, with maturation, hepatocytes lose it.
authors.affiliation: Second Department of Internal Medicine, Nagoya University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Y
authors.initials: Y
authors.lastname: Miyazawa
authors.forename: M
authors.initials: M
authors.lastname: Imoto
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama
authors.forename: I
authors.initials: I
authors.lastname: Nakano
authors.forename: H
authors.initials: H
authors.lastname: Nagura
authors.forename: K
authors.initials: K
authors.lastname: Kato
mesh_terms: D002908:Chronic Disease; D006521:Hepatitis, Chronic / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism* / Q000473:pathology; D008854:Microscopy, Electron; D010751:Phosphopyruvate Hydratase / Q000032:analysis*
chemical_list: D010751:Phosphopyruvate Hydratase