A screening for the presence of four different crystal protein gene types in 25 Bacillus thuringiensis strains.
25 different strains of Bacillus thuringiensis, belonging to 18 different serotypes were screened by Southern hybridization analysis for the presence of nucleotide sequences of four different gene types coding for crystal proteins showing different insecticidal spectra. Many but not all strains showed sequences homologous to any of the probes used. Homology with sequences of the gene type present in strain kurstaki HD1 occurred in all positive strains, whereas the other gene types were much less abundant. Sequences homologous to those of gene type VI, coding for a Spodoptera-specific crystal protein, appeared only in strains of serotypes aizawai and entomocidus.
Visser
B
B
Research Institute Ital, Wageningen, The Netherlands.
D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D015183:Restriction Mapping; D012703:Serotyping; D013045:Species Specificity
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis