Acid-base status of the newborn in relation to cord blood serum creatine kinase isoenzymes' activities.
Creatine kinase (CK) isoenzymes are considered to be specific tissue injury markers. The purpose of this study was to evaluate CK isoenzymes (CK-MM, CK-MB, and CK-BB) in umbilical cord blood sera of newborns in relation to their acid-base status. Investigations were performed in a group of 75 newborns delivered after 37 weeks of pregnancy. We estimated CK-isoenzymes with the use of DEAE-Sepharose C1-6B column chromatography. Total CK activity was measured using kits supplied by Boehringer-Mannheim (Monotest-R CK-NAC aktiviert). Newborns were considered hypoxic if umbilical artery blood pH was under 7.2 and/or base excess less than -10 mmol/L. Of 75 newly born infants 26 had features of perinatal hypoxia. We were unable to demonstrate significant differences in total CK, CK-MM and CK-MB activities in examined groups of newborns. We found a significant rise of CK-BB activity in cord sera of hypoxic infants. Our results show that the fetal brain is the most vulnerable to a hypoxic state.
Nikliński
W
W
Institute of Obstetrics and Gynecology, Medical Academy, Białystok, Poland.
Jóźwik
M
M
Pałynyczko
Z
Z
Bartnicki
J
J
Urban
J
J
D000136:Acid-Base Equilibrium; D015415:Biomarkers / Q000097:blood*; D003402:Creatine Kinase / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000032:analysis*; D005311:Fetal Hypoxia / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology; D006801:Humans; D007231:Infant, Newborn / Q000097:blood*; D007527:Isoenzymes; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D011247:Pregnancy
D015415:Biomarkers; D007527:Isoenzymes; D003402:Creatine Kinase