Iron-binding proteins and 67Ga accumulation by tumor cells.
Tumor cells incubated with 67Ga-citrate plus lactoferrin showed a lactoferrin concentration-dependent increase of 67Ga uptake while for transferrin the effect was an inhibition. A correlation was observed between the binding of 131I-labelled lactoferrin or 131I-labelled transferrin and the uptake of 67Ga by the cells. Saturation with iron did not change significantly the effects of the iron-binding proteins. Preincubation with lactoferrin, transferrin or ferric citrate increased 67Ga uptake. The formation of a ternary complex in which 67Ga mimics iron is discussed.
Anghileri
L J
LJ
Nuclear Medicine Service, Faculty of Medicine, University of Nancy, France.
Thouvenot
P
P
Crone-Escanye
M C
MC
Brunotte
F
F
Robert
J
J
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002951:Citrates / Q000493:pharmacokinetics*; D019343:Citric Acid; D003864:Depression, Chemical; D066298:In Vitro Techniques; D007781:Lactoferrin / Q000494:pharmacology*; D007782:Lactoglobulins / Q000494:pharmacology*; D009374:Neoplasms, Experimental / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000378:metabolism; D013268:Stimulation, Chemical; D014168:Transferrin / Q000494:pharmacology*
D002951:Citrates; D007782:Lactoglobulins; D014168:Transferrin; D019343:Citric Acid; D007781:Lactoferrin