title: Site-directed inactivation of human lung acidic glutathione S-transferase by 1-chloro-2,4-dinitrobenzene in the absence of glutathione.
abstract: Human lung acidic glutathione S-transferase is irreversibly inhibited by 1-chloro-2,4-dinitrobenzene (CDNB) in the absence of the co-substrate glutathione (GSH). The time-dependent inactivation is pseudo-first-order and demonstrates saturation kinetics, suggesting that inactivation occurs from an EI complex. The Ki was 0.14 mM; and kobs was 0.32 min-1 at 0.6 mM CDNB. The enzyme was protected against CDNB inactivation by GSH. The other two classes of glutathione S-transferase, the basic and near-neutral, are not significantly inactivated by CDNB. Incubation with [14C]CDNB indicated covalent binding to all three classes of transferase. One peptide fraction was found to be radiolabelled in both the basic and acidic transferases when these were incubated with [14C]CDNB and GSH, cleaved with cyanogen bromide, and chromatographed by HPLC. Incubation in the absence of GSH yielded one and two additional labelled peptide fractions for the basic and acidic transferases, respectively. Our results suggest that while CDNB arylates all three classes of human transferases, only the acidic transferase possesses a specific GSH-sensitive CDNB binding site, binding to which leads to time-dependent inactivation.
authors.affiliation: Department of Medicine, University of Cape Town Medical School, Observatory, South Africa.
authors.forename: A V
authors.initials: AV
authors.lastname: Corrigall
authors.forename: M M
authors.initials: MM
authors.lastname: Bhargava
authors.forename: K M
authors.initials: KM
authors.lastname: Ivanetich
authors.forename: M R
authors.initials: MR
authors.lastname: Ehlers
authors.forename: R E
authors.initials: RE
authors.lastname: Kirsch
mesh_terms: D001665:Binding Sites; D004137:Dinitrochlorobenzene / Q000378:metabolism / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology*; D011485:Protein Binding
chemical_list: D004137:Dinitrochlorobenzene; D005982:Glutathione Transferase; D005978:Glutathione