Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer.
A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.
Underhill
T M
TM
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
Flintoff
W F
WF
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D005816:Genetic Complementation Test; D006801:Humans; D008727:Methotrexate / Q000493:pharmacokinetics* / Q000494:pharmacology; D009154:Mutation; D010053:Ovary; D014162:Transfection
D004247:DNA; D008727:Methotrexate