title: Binding of complement component C5 to model immune complexes and the use of anti-C5 antibodies for determination of C5-containing circulating immune complexes.
abstract: When normal human or mouse serum is added to micro ELISA plates coated with monomeric or aggregated IgG, complement component C5 binds to IgG. C5 binding was demonstrated with a specific chicken anti-C5 antibody. Hydrazine treatment of the serum or addition of EDTA to the serum abolished the binding of C5. C5-deficient mouse serum was negative for C5 binding, whereas the same serum supplemented with human C5 restored the binding of C5. Chicken anti-C5-coated plates were used for determination of C5-containing circulating immune complexes (CIC). Increased concentrations of CIC were found in sera from patients with rheumatoid arthritis and Bell's palsy.
authors.affiliation: Department of Medical and Physiological Chemistry, Biomedical Centre, Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Larsson
authors.forename: J
authors.initials: J
authors.lastname: Sjöquist
mesh_terms: D000936:Antigen-Antibody Complex / Q000032:analysis / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D015153:Blotting, Western; D003182:Complement C5 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005158:Facial Paralysis / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011485:Protein Binding
chemical_list: D000936:Antigen-Antibody Complex; D003182:Complement C5