Expression of a mouse metallothionein gene in transgenic plant tissues.
Three gene constructions based on a mouse metallothionein I gene (mMT-I) were introduced into tobacco using a Ri plasmid vector system to test the effectiveness of animal gene regulatory signals in plant cells. No transcription from the native mouse gene was observed. In plant cells bearing chimeric mMT-I genes in which transcription was driven by the nopaline synthase promoter, neither polyadenylation nor splicing of mMT-I pre-mRNA was observed. Detailed comparisons of mMT-I sequences with those of known plant genes were carried out; slight differences in regions of known consensus sequences may be at least partly responsible for the non-recognition of mMT-I gene regulatory signals in plant cells, though other as yet unidentified, potentially necessary sequences may also be involved.
Pautot
V
V
Laboratoire de Biologie Cellulaire, INRA-Centre de Versailles, France.
Brzezinski
R
R
Tepfer
M
M
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004274:DNA, Recombinant; D005091:Exons; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D004247:DNA; D008668:Metallothionein