Relative specificities of inhibition of acid cholesteryl ester hydrolase and neutral cholesteryl ester hydrolase in cultured rabbit aortic smooth muscle cells by esterastin and cholesteryl oleyl ether.
Rabbit aortic smooth muscle cells in culture were incubated with 0.04-500 M esterastin. Acid cholesteryl ester hydrolase (ACEH) and neutral cholesteryl ester hydrolase (NCEH) activities were inhibited to a comparable degree, with 50% inhibition occurring in the range of 0.4 M esterastin. Cells incubated with cholesteryl oleyl ether showed 50% inhibition of NCEH at 5.0 M, but no inhibition of ACEH over a concentration range of 0.2-20 M. This relative specificity of cholesteryl oleyl ether for NCEH can be employed to study the relative roles of ACEH vs. NCEH in preventing cellular cholesteryl ester accumulation.
Morin
R J
RJ
Department of Pathology, Harbor-UCLA Medical Center, Torrance 90509.
Peng
S K
SK
D000818:Animals; D001011:Aorta; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007783:Lactones / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D011817:Rabbits; D002787:Sterol Esterase / Q000037:antagonists & inhibitors*
D007783:Lactones; C023511:cholesteryl oleyl ether; C017293:esterastin; D002784:Cholesterol; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase