title: Diagnostic screening of multiple antigen-antibody reactions in a new single assay on nitrocellulose: the line immunobinding assay (LIBA).
abstract: A new nitrocellulose-based ELISA technique, the line immunobinding assay (LIBA), permits the quantitative detection of multiple antigen-antibody reactions in one single assay. The antigen solutions are sprayed in thin lines onto a nitrocellulose sheet which is then cut into several strips before incubation in a multi-channel tray with serially diluted serum. The reacting antibodies are visualized by a peroxidase system. Comparison with other techniques routinely used for the detection of antibodies against measles, mumps and rubella, suggest that the LIBA offers specificity with sensitivity and may have a broad range of applications.
authors.affiliation: Institute for Immunology and Virology, University of Zurich, Switzerland.
authors.forename: R E
authors.initials: RE
authors.lastname: Aeppli
authors.forename: M J
authors.initials: MJ
authors.lastname: Bargetzi
authors.forename: H
authors.initials: H
authors.lastname: Binz
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D003101:Collodion; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008459:Measles virus / Q000276:immunology; D009109:Mumps virus / Q000276:immunology; D012412:Rubella virus / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D003101:Collodion