[Differentiation of pancreatic lipase and pseudolipase serum activity].
The determination of the serum lipase activity by turbidimetric assay was modified and false-positive results due to pseudolipase presence were eliminated. The new method includes a preincubation with beta-mercaptoenthanol and its conditions are specified to use with kinetic analysers as LKB 2086. By using our new method we estimated the activity of the pseudolipase in 36.4% of the group of 85 patients with elevated serum lipase activity determined by standard turbidimetry. By introducing our differential method with beta-mercaptoethanol correction the increasing correlation about 27% between lipase activity and serum alpha-amylase activity was estimated.
Kocna
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P
Slabý
J
J
D005189:False Positive Reactions; D006801:Humans; D008049:Lipase / Q000097:blood*; D009391:Nephelometry and Turbidimetry; D010179:Pancreas / Q000201:enzymology*
D008049:Lipase
Rozlisení aktivity pankreatické lipázy a pseudolipázy krevního séra.