title: Analysis of sister-chromatid exchanges, cell kinetics and mitotic index in lymphocytes of smoking pesticide sprayers.
abstract: Whole blood of 50 smokers who were exposed to pesticides was set up in RPMI 1640 medium, and observed for sister-chromatid exchanges (SCEs), cell kinetics (CK) and mitotic index (MI). As controls, blood samples were collected from 20 non-smokers (control I) and 27 smokers (control II) who were not exposed to pesticides. A significant increase in SCEs was observed as the duration of exposure increased. The frequency of M1 metaphases increased significantly whereas M2 and M3+ metaphases decreased in the exposed group. The mitotic index increased in control II and in the exposed population while it showed a decrease at 11-25 years' exposure.
authors.affiliation: Institute of Genetics, Osmania University, Andhra Pradesh, India.
authors.forename: D S
authors.initials: DS
authors.lastname: Rupa
authors.forename: P P
authors.initials: PP
authors.lastname: Reddy
authors.forename: O S
authors.initials: OS
authors.lastname: Reddi
mesh_terms: D000328:Adult; D000382:Agricultural Workers' Diseases / Q000235:genetics*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007306:Insecticides / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects*; D008875:Middle Aged; D008938:Mitosis / Q000187:drug effects*; D008940:Mitotic Index / Q000187:drug effects*; D009943:Organophosphorus Compounds; D010575:Pesticides / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D012907:Smoking / Q000009:adverse effects*
chemical_list: D007306:Insecticides; D009943:Organophosphorus Compounds; D010575:Pesticides; D011722:Pyrethrins