Carp growth hormone: molecular cloning and sequencing of cDNA.
cDNA clones of the fish Cyprinus carpio growth hormone (GH) mRNA have been isolated from a cDNA library prepared from carp pituitary gland poly(A)+RNA. The nucleotide sequence of one of the carp GH cDNA clones containing an insert of 1164 nucleotides (nt) was determined. The cDNA sequence was found to encode a polypeptide of 210 amino acids (aa) including a signal peptide of 22 aa and to contain 5' and 3' untranslated regions of the mRNA of 36 and 498 nt, respectively. The carp GH presents a 63% amino acid sequence homology with the salmon GH, has structural features common with other GH polypeptides of mammalian or avian origin and contains domains of conserved sequence near the N- and C-terminal regions. Southern blot hybridization of carp genomic DNA with GH cDNA probes shows the presence of at least two GH-coding sequences in the fish genome.
Koren
Y
Y
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Sarid
S
S
Ber
R
R
Daniel
V
V
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002347:Carps / Q000235:genetics*; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005796:Genes; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012474:Salmon / Q000235:genetics
D011498:Protein Precursors; D012333:RNA, Messenger; D013006:Growth Hormone; D004247:DNA