[Isolation of low-molecular weight fractions from the splenic tissue of calves that inhibit the proliferation of leukemic cells in mice].
The three-stage scheme of extracting the low-molecular weight biologically active substances from spleen tissue has been suggested. It includes the following steps: adsorption chromatography on hydroxyapatite, ion-exchange chromatography on DEAE-matrices and gel-filtration. The resulting pool of low-molecular substances inhibits the 3H-thymidine incorporation into DNA of cultured leukemic cells.
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D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects; D003864:Depression, Chemical; D007942:Leukemia, Experimental / Q000473:pathology*; D008722:Methods; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D013154:Spleen / Q000276:immunology*; D014020:Tissue Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D014020:Tissue Extracts
Poluchenie nizkomolekuliarnykh fraktsiĭ iz tkani selezenki teliat, ingibiruiushchikh proliferatsiiu leĭkoznykh kletok mysheĭ.