[Improved method of purification of L-lysine-alpha-oxidase from the fungus Trichoderma sp].
Relatively rapid procedure was developed for isolation and purification of the antitumor enzyme L-lysine alpha-oxidase. The procedure involved three steps instead of six or four steps described previously, with a yield of 64.4%. The homogenous preparation was obtained as shown by gel electrophoresis and ultracentrifugation. The antitumor activity of the enzyme both in vivo and in vitro is under investigation.
Iankevich
N B
NB
Laugalene
N F
NF
Vesa
V S
VS
Khaduev
S Kh
SKh
Smirnova
I P
IP
D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D050830:L-Amino Acid Oxidase; D003904:Mitosporic Fungi / Q000201:enzymology*; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology*
D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase
Usovershenstvovannaia skhema ochistki L-lizin-alpha-oksidazy griba Trichoderma sp.