title: The human immunodeficiency virus rev protein is a nuclear phosphoprotein.
abstract: The human Immunodeficiency virus rev protein is one of several key regulatory proteins involved in the control of viral structural protein synthesis and replication. In this report, we identify the 20-kDa rev protein as a nuclear phosphoprotein. Post-translational modification was observed solely on serine residues. In vitro kinase reactions utilizing a derivative of Rev purified from Escherichia coli identified a nuclear kinase capable of phosphorylating exogenously added rev protein. Our results suggest that the nuclear kinase activity observed in vitro is likely to be responsible for the in vivo post-translational modification of Rev.
authors.affiliation: Department of Molecular Oncology, Roche Institute of Molecular Biology, Nutley, New Jersey.
authors.forename: A
authors.initials: A
authors.lastname: Cochrane
authors.forename: R
authors.initials: R
authors.lastname: Kramer
authors.forename: S
authors.initials: S
authors.lastname: Ruben
authors.forename: J
authors.initials: J
authors.lastname: Levine
authors.forename: C A
authors.initials: CA
authors.lastname: Rosen
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D006678:HIV / Q000378:metabolism*; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000502:physiology*; D010750:Phosphoproteins / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012191:Retroviridae Proteins / Q000502:physiology*
chemical_list: D009687:Nuclear Proteins; D010750:Phosphoproteins; D012191:Retroviridae Proteins; D011494:Protein Kinases