title: In vitro effect of human growth hormone on lymphocyte transformation and lymphocyte growth factors secretion.
abstract: Cultures of human blood peripheral lymphocytes were performed in the presence or absence of human growth hormone, and also of phytohemagglutinin and normal human serum 10%. After incubation for 48 h, the supernatants were tested for their ability to promote the uptake of [3H]thymidine into lectin-activated lymphocytes. Supernatants from lymphocyte-free control samples, treated in the same manner, were assayed under the same experimental conditions. Variance analysis of the different dose-response relationships was performed. The results of these in vitro experiments confirm that physiological levels of GH inhibit the lectin-induced lymphoproliferation and that lymphocytes secrete an 'activity' able to stimulate the incorporation of [3H]thymidine into lectin activated lymphocytes. Furthermore we show that: 1) Secretion of this lymphocyte-stimulating activity is increased by physiological levels of GH; 2) This lymphocytic secretion is not radioimmunoassayable IGF-I; 3) Using fast protein liquid chromatography (FPLC), this activity appears in fractions with various molecular weights.
authors.affiliation: INSERM U 188, Paris, France.
authors.forename: R M
authors.initials: RM
authors.lastname: Schimpff
authors.forename: A M
authors.initials: AM
authors.lastname: Repellin
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D002850:Chromatography, Gel; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007378:Interleukins / Q000032:analysis / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008222:Lymphokines / Q000032:analysis / Q000096:biosynthesis*; D008970:Molecular Weight
chemical_list: D007378:Interleukins; D008222:Lymphokines; C043695:lymphocyte proliferation potentiating factors; D013006:Growth Hormone