Analysis of porcine erythrocyte lysate proteins by horizontal two-dimensional electrophoresis with immobilized pH gradient in the first dimension.
Horizontal two-dimensional gel electrophoresis with immobilized pH gradients in the first dimension resulted in excellent patterns of porcine erythrocyte lysate proteins. The overwhelming excess of hemoglobin was removed by chloroform-ethanol extraction and the number of spots further increased by acetone precipitation, revealing considerably more protein spots than detected by analyzing the untreated sample. A total of 430 protein spots have been reproducibly obtained for further analysis of genetic variations. A number of enzyme systems have been identified by visual inspection and comparison with previous results. The existence of further multiple forms for other enzymes is also apparent.
Russ
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I
Lehrstuhl für Tierzucht, Technische Universität München, Freising--Weihenstephan, Federal Republic of Germany.
Günther
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S
Pirchner
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F
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004912:Erythrocytes / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013552:Swine / Q000097:blood
D001798:Blood Proteins