Effect of fraction V powder from bovine serum on rat liver microsomal glutathione S-transferase activity.
When bovine serum fraction V powder, which contains 98-99% albumin, is incubated with rat liver microsomes, the glutathione S-transferase activity is stimulated 3-fold. The fraction V stimulates the transferase activity even in microsomes pretreated with N-ethylmaleimide. There is no such stimulating activity in protein fractions other than fraction V. The corresponding cytosolic enzyme activity is not changed by fraction V powder or by N-ethylmaleimide. Neither treatment with charcoal nor heating in boiling water for 5 min can remove the stimulating activity from fraction V.
Nishino
H
H
Department of Immunology, School of Medicine, University of Occupational and Environmental Health, Japan.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001769:Blood; D002606:Charcoal / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D019803:Glutathione Disulfide; D005982:Glutathione Transferase / Q000378:metabolism*; D006358:Hot Temperature; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010455:Peptides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D000418:Albumins; D010455:Peptides; D002606:Charcoal; D005982:Glutathione Transferase; D005978:Glutathione; D005033:Ethylmaleimide; D019803:Glutathione Disulfide