title: Age related changes in hemopoietic capacity of bone marrow cells.
abstract: The effect of aging on the hemopoietic capacity of bone marrow (BM) cells was investigated. No difference was found in the incidence of colony forming units-spleen (CFU-S) and granulocyte-macrophage colony forming cells (GM-CFC) present in the BM of young (2-4 months) or old (24-30 months) mice. However, increased proliferation (x3) of the old BM cells was observed when cultured in the presence of L-cell conditioned medium. The cells were also cultivated over an adherent layer of a BM stroma cell line (14F1.1, endothelial-adipocytes) and the non-adherent cells removed and tested weekly. Cells originating from the old BM proliferated to a greater extent and produced more GM-CFC than those from the young. Differentiation into T-cells after colonizing fetal thymus explants was also measured and found to be reduced in both groups, though to a greater extent in the old. Thus, under the present experimental conditions, myeloid progenitors in the old BM manifest a more pronounced self renewal and differentiation capacity than the young while for the T-cell progenitors the situation is reversed.
authors.affiliation: Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Sharp
authors.forename: D
authors.initials: D
authors.lastname: Zipori
authors.forename: J
authors.initials: J
authors.lastname: Toledo
authors.forename: S
authors.initials: S
authors.lastname: Tal
authors.forename: P
authors.initials: P
authors.lastname: Resnitzky
authors.forename: A
authors.initials: A
authors.lastname: Globerson
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001853:Bone Marrow / Q000276:immunology / Q000502:physiology*; D002478:Cells, Cultured; D006098:Granulocytes / Q000502:physiology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D013154:Spleen / Q000502:physiology; D013950:Thymus Gland / Q000502:physiology