Hyperammonemia decreases body fat content in rat.
We have developed an animal model of hyperammonemia consisting of feeding rats a diet containing 20% (w/w) ammonium acetate. Ingestion of this diet markedly affects carcass composition, with a 46% reduction in lipid content. The ammonium diet alters levels of several key compounds involved in lipid metabolism. Long-chain acylcarnitine is increased in liver by approx. 60% while free carnitine and acetylcarnitine are unaffected. The hepatic content of acetyl-CoA increases by approx. 50%. The level of ketone bodies in blood increases by 32% but remains unchanged in liver. Our data indicate that hyperammonemia alters lipid metabolism and results in a significant decrease in body lipid content.
Miñana
M D
MD
Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Centro Asociado de CSIC, Spain.
Felipo
V
V
Wallace
R
R
Grisolía
S
S
D000085:Acetates / Q000008:administration & dosage; D000105:Acetyl Coenzyme A / Q000032:analysis; D000108:Acetylcarnitine / Q000032:analysis; D000273:Adipose Tissue / Q000378:metabolism*; D000641:Ammonia / Q000097:blood*; D000818:Animals; D002331:Carnitine / Q000032:analysis / Q000097:blood; D007657:Ketone Bodies / Q000032:analysis / Q000097:blood; D008099:Liver / Q000032:analysis; D008297:Male; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D007657:Ketone Bodies; D000108:Acetylcarnitine; D000105:Acetyl Coenzyme A; D000641:Ammonia; C018824:ammonium acetate; D002331:Carnitine