Drug-related chromosomal changes in chemoresistant human ovarian carcinoma cells.
Four resistant sublines were derived from the sensitive human ovarian carcinoma IGROV 1 (OV1-P) cell line by exposure to increasing concentrations of vincristine, doxorubicin, and cisplatinum. The vincristine-resistant sublines expressed the MDR phenotype associated with a complete reversion of malignant properties. Cytogenetic studies of sensitive and resistant cells have been repeatedly performed over a 1-year period. Consistent and stable drug-related chromosomal structural rearrangements have been observed in each resistant population affecting chromosomes 3, 4, 6, 8, 11, 22, and X. The most significant result was the presence in OV1/P cells of a minor subclone with a del(11)(p13) marker that represented the whole OV1/VCR population. This result suggests a possible role of this deletion either in the drug-selection process, or in the malignant reversion.
Teyssier
J R
JR
Laboratoire d'Histologie-Embryologie-Cytogénétique. INSERM U 314, Faculté de Médecine, Reims, France.
Bénard
J
J
Ferre
D
D
Da Silva
J
J
Renaud
L
L
D000230:Adenocarcinoma / Q000235:genetics*; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D004351:Drug Resistance / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D010051:Ovarian Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D005819:Genetic Markers