Enzyme assay of L-myo-inositol 1-phosphate synthetase based on high-performance liquid chromatography of benzoylated inositol.
A procedure for the determination of inositol by reversed-phase HPLC is described which is based on a precolumn benzoylation and detection at 230 nm. This procedure was used to assay the activity of L-myo-inositol 1-phosphate synthetase (EC 5.5.1.4) after treatment of the enzymatic product by a phosphatase.
Feth
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F
Ag. Enzymologie, GBF, Gesellschaft für Biotechnologische Forschung, Braunschweig, Federal Republic of Germany.
Wagner
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R
Baydoun
H
H
Wagner
K G
KG
D001565:Benzoates / Q000032:analysis*; D002238:Carbohydrate Epimerases / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007294:Inositol / Q000031:analogs & derivatives* / Q000032:analysis; D007296:Myo-Inositol-1-Phosphate Synthase / Q000032:analysis*; D015003:Yeasts / Q000201:enzymology
D001565:Benzoates; D007294:Inositol; D002238:Carbohydrate Epimerases; D007296:Myo-Inositol-1-Phosphate Synthase