Analysis of heparins by size-exclusion and reversed-phase high-performance liquid chromatography with photodiode-array detection.
High-performance liquid chromatography (HPLC) of heparins was carried out with on-line photodiode-array detection. Average molecular weights and molecular weight distribution were calculated using computer data acquisition and handling; size-exclusion chromatography was performed using different selective microparticulate columns and narrow molecular weight distribution heparin standards for calibration; heparin peak purity was investigated using several methods for evaluation. Additional UV-absorbing compounds present in heparin preparations were characterized and quantitated by reversed-phase HPLC. These methods are useful for the analysis of the molecular weight distribution and peak purity of heparins and for the determination of additional drugs, additives or impurities.
de Vries
J X
JX
Abteilung für Klinische Pharmakologie, Medizinische Klinik, Universität Heidelberg, F.R.G.
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006493:Heparin / Q000032:analysis*; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet
D006493:Heparin