Soluble non-collagen proteins of bovine vitreous humour.
Bovine vitreous humour contains 0.6 +/- 0.04 mg of proteins per cm3. Three main protein fractions were obtained by CM-cellulose chromatography and their homogeneity was confirmed by isoelectric focusing and SDS-gel electrophoresis. The Mr and pl values of the separated fractions were: fraction I, 67,000 and 4.02; fraction II, 59,000 and 5.22; fraction III, 43,000 and 7.48, respectively.
Wolańska
M
M
Department of Biochemistry, Medical Academy, Poland.
Bańkowski
E
E
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000302:isolation & purification*; D007526:Isoelectric Point; D014822:Vitreous Body / Q000032:analysis*
D000596:Amino Acids; D005136:Eye Proteins