title: Acid-base status of the newborn in relation to cord blood serum creatine kinase isoenzymes' activities.
abstract: Creatine kinase (CK) isoenzymes are considered to be specific tissue injury markers. The purpose of this study was to evaluate CK isoenzymes (CK-MM, CK-MB, and CK-BB) in umbilical cord blood sera of newborns in relation to their acid-base status. Investigations were performed in a group of 75 newborns delivered after 37 weeks of pregnancy. We estimated CK-isoenzymes with the use of DEAE-Sepharose C1-6B column chromatography. Total CK activity was measured using kits supplied by Boehringer-Mannheim (Monotest-R CK-NAC aktiviert). Newborns were considered hypoxic if umbilical artery blood pH was under 7.2 and/or base excess less than -10 mmol/L. Of 75 newly born infants 26 had features of perinatal hypoxia. We were unable to demonstrate significant differences in total CK, CK-MM and CK-MB activities in examined groups of newborns. We found a significant rise of CK-BB activity in cord sera of hypoxic infants. Our results show that the fetal brain is the most vulnerable to a hypoxic state.
authors.affiliation: Institute of Obstetrics and Gynecology, Medical Academy, Białystok, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Nikliński
authors.forename: M
authors.initials: M
authors.lastname: Jóźwik
authors.forename: Z
authors.initials: Z
authors.lastname: Pałynyczko
authors.forename: J
authors.initials: J
authors.lastname: Bartnicki
authors.forename: J
authors.initials: J
authors.lastname: Urban
mesh_terms: D000136:Acid-Base Equilibrium; D015415:Biomarkers / Q000097:blood*; D003402:Creatine Kinase / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000032:analysis*; D005311:Fetal Hypoxia / Q000097:blood / Q000175:diagnosis* / Q000201:enzymology; D006801:Humans; D007231:Infant, Newborn / Q000097:blood*; D007527:Isoenzymes; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D011247:Pregnancy
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D003402:Creatine Kinase