title: Nile red simultaneous staining of intracellular lipids and membrane network in human muscle cultures.
abstract: We have applied a new fluorescent probe, Nile red, on normal and pathological human muscle derived cultures and compared the results with corresponding human muscle sections. In normal human muscle cultures, Nile red strain has proved useful for visualization of both intracellular lipids and membrane network. Similar patterns have been observed in muscle cultures derived from lipid storage and mitochondrial myopathies. Moreover, abnormalities in pathological muscle cultures could be revealed by establishing more advanced culture systems.
authors.affiliation: Istituto di Clinica Neurologica, Centro Dino Ferrari, Università degli Studi, Milano, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Santilli
authors.forename: A
authors.initials: A
authors.lastname: Prelle
authors.forename: L
authors.initials: L
authors.lastname: Geremia
authors.forename: G
authors.initials: G
authors.lastname: Scarlato
authors.forename: G
authors.initials: G
authors.lastname: Meola
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D008055:Lipids / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis*; D009132:Muscles / Q000032:analysis* / Q000166:cytology / Q000473:pathology; D009135:Muscular Diseases / Q000378:metabolism* / Q000473:pathology; D010078:Oxazines
chemical_list: D008055:Lipids; D008563:Membrane Lipids; D010078:Oxazines; C044808:nile red