Micronucleus test with potassium chromate(VI) administered intraperitoneally and orally to mice.
The effect of route of administration, intraperitoneal (i.p.) or oral gavage (p.o.), in the mouse micronucleus test was studied with K2CrO4 in 2 mouse strains (MS/Ae and CD-1). A simplified acute toxicity test to estimate the toxic dose levels of K2CrO4 showed that the LD50S were 50 mg/kg i.p. and 300 mg/kg p.o. for MS/Ae and 32 mg/kg i.p. and 180 mg/kg p.o. for CD-1. Based on results of a pilot micronucleus test to determine appropriate dose levels and the optimal sampling time, it was decided to sample bone marrow cells of both strains of mice 24 h after i.p. doses of 10-80 mg/kg and p.o. doses ranging from 20 to 320 mg/kg. K2CrO4 administered i.p. induced micronucleated polychromatic erythrocytes (MNPCEs) dose-dependently in both strains. In contrast, when administered p.o. the chemical failed to induce MNPCEs. These results suggest that this difference between i.p. and p.o. routes is related to a difference of absorption or metabolic fate of chromate in vivo.
Shindo
Y
Y
Pharmacology and Toxicology Laboratories, Meiji Seika Kaisha Ltd., Yokohama, Japan.
Toyoda
Y
Y
Kawamura
K
K
Kurebe
M
M
Shimada
H
H
Hattori
C
C
Satake
S
S
D000284:Administration, Oral; D000818:Animals; D002840:Chromates / Q000008:administration & dosage* / Q000633:toxicity; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage*; D010865:Pilot Projects; D017680:Potassium Compounds
D002840:Chromates; D009153:Mutagens; D017680:Potassium Compounds; C027373:potassium chromate(VI)