Regulation of expression of the c-sis proto-oncogene.
Regulation of expression of platelet derived growth factor polypeptide B encoded by the c-sis proto-oncogene is important in a number of physiological and pathological conditions. Sequences in the 1028 nucleotide long 5' untranslated region of the c-sis mRNA were found to inhibit protein synthesis. The inhibition is relieved by deletion of nucleotides 154-378 or 398-475. Sequences within 375 nucleotides upstream of the RNA initiation site are important for transcriptional activity. Sequences in two portions of this region, between -375 and -235 nucleotides and between -235 and -99 nucleotides relative to the RNA CAP site are important for full activity. A transcriptional enhancer activity is demonstrated by its ability to increase the activity of the human T lymphotropic virus type (HTLV) I promoter at a distance and in an orientation-independent manner. Furthermore, sequences upstream of the c-sis RNA CAP site respond to the HTLV I transactivator protein to increase RNA synthesis from either the c-sis or HTLV I promoter.
Ratner
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Department of Medicine, Washington University, St Louis, MO 63110.
D001482:Base Composition; D001483:Base Sequence; D001667:Binding, Competitive; D015500:Chloramphenicol O-Acetyltransferase; D002872:Chromosome Deletion; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000502:physiology; D012191:Retroviridae Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D012191:Retroviridae Proteins; D014157:Transcription Factors; D015500:Chloramphenicol O-Acetyltransferase