In vitro production of viable bacteriophage in a laser plume.
Bacteriophage phi X 174, present at high concentrations in an agar overlay in vitro system, were used as a target for pulsed 2.94 microns (Er:YAG) laser ablation. In this preliminary experiment, the potential for transport of viable viruses in the photoablation plume was investigated for laser energy densities of approximately 3.5 J/cm2 per pulse in exposures of 75-600 laser pulses. Transport of relatively few numbers of viable viruses from the ablation site to the plate assay detector, a distance of approximately 2 cm, was observed.
Ediger
M N
MN
Center for Devices and Radiological Health, Office of Science and Technology, Rockville, Maryland 20857.
Matchette
L S
LS
D000336:Aerosols; D000391:Air Microbiology; D003090:Coliphages / Q000302:isolation & purification*; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers
D000336:Aerosols