title: Chromosomal aberration and sister-chromatid exchange frequencies in peripheral blood lymphocytes of a large human population sample. II. Extension of age range.
abstract: We have previously reported on a cytogenetic-epidemiological study of chromosomal aberration and sister-chromatid exchange (SCE) frequencies in peripheral blood lymphocytes (PBL) of a cohort of 353 healthy employees of the Brookhaven National Laboratory (Bender et al., 1988). This sample has now been increased in order to extend the age range represented and, incidentally, the representation of non-white subjects. In total, the data now include chromosomal aberration information from 108,950 cells and SCE information from 25,397 cells from 613 samples from 493 subjects. Neither the mean frequencies of any of the chromosomal aberration types nor the mean frequency of SCE have changed notably through the addition of the new subjects and samples. The mean age at sampling of the population is now 43.1 years with a range of from 1.1 to 83.7 years. However, we still find no significant relationship of the frequency of any conventional aberration category to age with the single exception of the dicentric chromosome, which now shows a positive regression (p = 0.001). The raw mean SCE frequencies show a statistically significant increase with subject age, but when cigarette smoking status is taken into account, no significant age relationship is found. As with the earlier samples, neither aberration nor SCE frequencies was influenced by race. Mean SCE frequencies, measured in non-smokers, were about 5% higher in females than males. Only one aberration category, "supernumerary acentric fragment", was significantly related to sex. This "aberration", known to constitute early centromere separation of an X chromosome, is much more common in females than in males and, in the females, increases significantly with increasing subject age.
authors.affiliation: Medical Department, Brookhaven National Laboratory, Upton, NY 11973.
authors.forename: M A
authors.initials: MA
authors.lastname: Bender
authors.forename: R J
authors.initials: RJ
authors.lastname: Preston
authors.forename: R C
authors.initials: RC
authors.lastname: Leonard
authors.forename: B E
authors.initials: BE
authors.lastname: Pyatt
authors.forename: P C
authors.initials: PC
authors.lastname: Gooch
mesh_terms: D000293:Adolescent; D000367:Age Factors; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D006801:Humans; D007223:Infant; D008214:Lymphocytes; D008875:Middle Aged; D009154:Mutation; D044469:Racial Groups; D012854:Sister Chromatid Exchange