Isolation of self-recognizing IgG2a monoclonal rheumatoid factors.
Hybridoma cell lines were produced by fusing spleen cells from 11 mice that expressed the lpr mutation to nonsecreting SP2/0 myeloma cells. Five to 20% of the hybridoma cell lines so derived were found to secrete antibodies that recognized autologous IgG2a. Out of 168 anti-IgG2a clones tested, 4 clones produced anti-IgG2a rheumatoid factor (RF) that was itself IgG2a. Two clones recognized self IgG2a while the other two clones each recognized a nonself IgG2a allotypic determinant. These findings indicate that self-recognizing antibodies are a component, albeit a minor one, of the RF repertoire of lpr/lpr mice.
Wolfowicz
C B
CB
Department of Microbiology, Boston University Medical Center, Massachusetts 02118.
Lyons
S
S
Mahon
C A
CA
Marshak-Rothstein
A
A
Rothstein
T L
TL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D007126:Immunoglobulin Allotypes / Q000032:analysis; D007127:Immunoglobulin Constant Regions / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D012217:Rheumatoid Factor / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007127:Immunoglobulin Constant Regions; D007074:Immunoglobulin G; D012217:Rheumatoid Factor