Oxidative metabolism of 4-hydroxy-2,3-nonenal during diethyl-nitrosamine-induced carcinogenesis in rat liver.
In some chemically-induced hepatomas and in cultured transformed cells the aldehyde dehydrogenase activity was found increased in the presence of aromatic aldehyde as substrate. We studied this enzyme during diethyl-nitrosamine carcinogenesis in rat liver by using an aliphatic aldehyde, 4-hydroxynonenal, as substrate. 4-Hydroxynonenal is an important product of lipid peroxidation. The NAD- and NADP-dependent aldehyde dehydrogenase of the cytosolic fraction and the NADP-dependent aldehyde dehydrogenase of the microsomes show higher values in nodules and hepatoma than in normal liver. These results suggest that increased aldehyde dehydrogenase, when 4-hydroxynonenal is used, can be considered a marker of the neoplastic process, in the same way as the level of aldehyde dehydrogenase increased in presence of aromatic aldehyde.
Canuto
R A
RA
Dipartimento di Medicina ed Oncologia Sperimentale, Universita di Torino, Italia.
Muzio
G
G
Biocca
M E
ME
Dianzani
M U
MU
D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D004052:Diethylnitrosamine; D008054:Lipid Peroxides / Q000096:biosynthesis; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D011230:Precancerous Conditions / Q000139:chemically induced / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344
D000447:Aldehydes; D008054:Lipid Peroxides; D009243:NAD; D004052:Diethylnitrosamine; D009249:NADP; D000444:Aldehyde Dehydrogenase; C027576:4-hydroxy-2-nonenal