title: The wet disc antimicrobial solution assay. An in vitro method to test efficacy of antimicrobial solutions for topical use.
abstract: The wet disc antimicrobial solution assay, an in vitro method to test susceptibility/resistance of burn wound-isolated microorganisms against antimicrobial solutions for topical use, is described. Where antibiotic discs for Kirby-Bauer testing were available for antibiotics contained in the topical solutions, Kirby-Bauer assays were done, and the results were compared with those derived using the wet disc assay. More microorganisms were susceptible using the wet disc method compared with the Kirby-Bauer method. The wet disc method could be used to test topical antimicrobial solutions that had no Kirby-Bauer disc equivalent. The wet disc method provided more reliable information for the clinician to use in choosing appropriate topical antimicrobial solutions for treating patients' wounds than the Kirby-Bauer method.
authors.affiliation: Department of Surgery, University of Cincinnati College of Medicine, Ohio.
authors.forename: I A
authors.initials: IA
authors.lastname: Holder
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002056:Burns / Q000150:complications; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests / Q000379:methods*; D014946:Wound Infection / Q000188:drug therapy
chemical_list: D000891:Anti-Infective Agents, Local