Blood oxygen binding properties for the burrowing owl, Athene cunicularia.
Isocapnic O2 equilibrium curves (O2ECs) were generated for whole blood of 4 adult burrowing owls (Athene cunicularia) using thin film techniques. At in vivo pHa (7.49 +/- 0.02; mean +/- 1 SEM) and 41 degrees C, the PO2 at half saturation (P50) was 42.3 +/- 0.8 Torr. CO2 and fixed acid (H+) Bohr slopes (delta log P50/delta pH) were -0.46 +/- 0.01 and -0.42 +/- 0.02, respectively, demonstrating a small specific CO2 effect. CO2 and H+ Bohr slopes were saturation-independent between 0.1 and 0.9 S. Hill plots for Athene blood were non-linear; the Hill coefficient (n) increased from 2.6 below 0.4 S to 3.4 above 0.6 S. Owl equilibrium data were accurately described by the equation: S = [(7.7 x 10(6]/(P4 + 44P3 - 108P2 + 3.5 x 10(4)P) + 1]-1. This complex O2EC shape may result from Hb heterogeneity; isoelectric focusing showed 4 isoHbs with a molar ratio of 9:1:1:1. This study revealed no apparent adaptations of Athene blood for hypoxic and hypercapnic conditions. We conclude that the observed blood O2 binding properties promote tissue O2 delivery during periods of surface activity. While occupying its burrow, the owl compensates for moderate alterations in inspired gas composition partly through increased ventilation.
Maginniss
L A
LA
Division of Biology and Medicine, Brown University, Providence, RI 02912.
Kilgore
D L
DL
D000136:Acid-Base Equilibrium; D000818:Animals; D001692:Biological Transport; D001717:Birds / Q000097:blood*; D004777:Environment; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000097:blood*; D010108:Oxyhemoglobins / Q000378:metabolism; D010710:Phosphates / Q000097:blood
D010108:Oxyhemoglobins; D010710:Phosphates; D010100:Oxygen