Antibody-forming cell assays of avian paramyxoviruses: the serotype-specific response of mice.
Mice were immunized with eight different serotypes of avian paramyxovirus (PMV). The number of splenocytes that formed antibody to PMV-infected Madin-Darby bovine kidney cells was determined by immunoperoxidase staining. Microscopic examination revealed brown plaques of cell surface or inclusion body-like antigen comprising the glycoprotein or nucleoprotein-polymerase complex, respectively. The IgG response to virus glycoprotein was priming-dependent and 99% serotype-specific, a value which exceeds that of PMV typing using avian serology. The response to inclusion body-like antigens was more variable and less priming-dependent and suggested cross-reactivities between PMV-1 and PMV-3 or -9. The primary IgM response also included a 1 to 10% non-specific antibody response to host antigens revealed by neuraminidase treatment.
Russell
P H
PH
Department of Veterinary Pathology, Royal Veterinary College, University of London, U.K.
Ozdemir
I
I
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D003429:Cross Reactions; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D007700:Kinetics; D051379:Mice; D010252:Paramyxoviridae / Q000145:classification / Q000276:immunology*; D012703:Serotyping; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000954:Antigens, Surface; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014759:Viral Envelope Proteins