title: Bioluminescent flow sensors: L-lactate dehydrogenase activity determination in serum.
abstract: The catalytic activity of serum L-lactate dehydrogenase (LDH), was determined by monitoring the NADH produced by LDH with bacterial bioluminescent enzymes immobilized on a nylon coil. The LDH reaction of L-lactate with NAD took place in a flow-through mixing coil that preceded the bioluminescent detector coil. The response was linear from 1 to 5000 U/l at 37 degrees C and from 3 to 2000 U/l at 25 degrees C. The intra- and inter-assay reproducibility (CV%) were less than 10% and recovery range was 92% to 110%. The results agreed well with those obtained with a spectrophotometric method.
authors.affiliation: Institute of Chemical Sciences, University of Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Girotti
authors.forename: C
authors.initials: C
authors.lastname: Bassoli
authors.forename: M L
authors.initials: ML
authors.lastname: Cascione
authors.forename: S
authors.initials: S
authors.lastname: Ghini
authors.forename: G
authors.initials: G
authors.lastname: Carrea
authors.forename: R
authors.initials: R
authors.lastname: Bovara
authors.forename: A
authors.initials: A
authors.lastname: Roda
authors.forename: R
authors.initials: R
authors.lastname: Motta
authors.forename: R
authors.initials: R
authors.lastname: Petilino
mesh_terms: D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008163:Luminescent Measurements; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D004800:Enzymes, Immobilized; D009243:NAD; D007770:L-Lactate Dehydrogenase