title: Changes in glycosaminoglycan sulfation and protein kinase C subcellular distribution during differentiation of the human colon tumor cell line Caco-2.
abstract: During the spontaneous differentiation (day 5 to day 15 of the culture) of Caco-2 cells, the sulfation of cell layer glycosaminoglycans increased, whereas protein kinase C activity was concomitantly redistributed from the membrane to the cytosol. The protein kinase C activators, 4 beta-phorbol 12 beta-myristate, 13 alpha-acetate and 1,2-dioctanoylglycerol inhibited glycosaminoglycan sulfation. By contrast, 4 alpha-phorbol 12, 13 didecanoate was ineffective. These results suggest that membrane-bound PKC may exert a modulatory effect on glycosaminoglycan sulfation, and this effect is gradually attenuated as Caco-2 cell differentiation progresses.
authors.affiliation: Laboratoire de Biochimie, INSERM, Faculté de Médecine Saint-Antoine, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Levy
authors.forename: G
authors.initials: G
authors.lastname: Cherqui
authors.forename: A
authors.initials: A
authors.lastname: Robert
authors.forename: D
authors.initials: D
authors.lastname: Wicek
authors.forename: J
authors.initials: J
authors.lastname: Picard
mesh_terms: D002454:Cell Differentiation; D002458:Cell Fractionation; D003110:Colonic Neoplasms; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D011493:Protein Kinase C / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D006025:Glycosaminoglycans; D013431:Sulfates; D011493:Protein Kinase C