[Isolation and characterization of gliadins from Aegilops squarrosa seeds].
The major gliadin components were isolated from the seeds of the diploid species Aegilops squarrosa, a putative source of polyploid wheat D-genome. The isolation procedure included gel-filtration and reversed-phase high-performance liquid chromatography (HPLC). The purified proteins were characterized by electrophoretic mobility in polyacrylamide gel using acid Al-lactate system and a system containing sodium dodecyl sulfate. The amino acid composition of isolated omega-gliadins was determined. Using covalent chromatography on thiopropyl-Sepharose 6B it was found that omega-gliadins of A. squarrosa contain no SH-groups and/or S-S-bonds. The N-terminal amino acid sequences of A. squarrosa gliadins were determined. omega-Gliadins were found to contain three types of N-terminal amino acid sequences, one of which, SRQ, in hexaploid wheat is encoded by 1B chromosome. It was shown that some omega-gliadins of A. squarrosa have blocked N-terminal amino acids. The major component of the gamma-fraction was found to contain an N-terminal sequence of gamma 2 type encoded in polyploid wheat by 1D chromosome. Gliadins with electrophoretic mobility in the beta-zone of the spectrum possess the N-terminal sequence of alpha-type. The results obtained are discussed in terms of the origin of polyploid wheat genomes.
Odintsova
T I
TI
Egorov
Ts A
TsA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005903:Gliadin / Q000032:analysis / Q000302:isolation & purification*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000302:isolation & purification*; D011003:Ploidies; D006109:Poaceae / Q000032:analysis* / Q000235:genetics; D012639:Seeds / Q000032:analysis*; D014908:Triticum / Q000032:analysis* / Q000235:genetics; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D010940:Plant Proteins; D005903:Gliadin; D014357:Trypsin
Vydelenie i kharakteristika gliadinov iz semian Aegilops squarrosa.