Platelet-melanoma cell interaction is mediated by the glycoprotein IIb-IIIa complex.
A human malignant melanoma cell line (M3Dau) was observed by electron microscopy to interact directly with human platelets and induced platelet aggregation. Fab fragments of a monoclonal antibody MoAb (LYP18), directed against the platelet glycoprotein (GP) IIb-IIIa complex, inhibited platelet-melanoma interactions and platelet-platelet aggregation. M3Dau melanoma cells bind LYP 18 and synthesize IIb-IIIa-like GPs. When the melanoma cells were preincubated with LYP 18, tumor-platelet interaction did not occur, suggesting that the interaction may be mediated by the IIb-IIIa-like GPs present on the melanoma cell surface. Glanzmann's thrombasthenic platelets, lacking GPIIb and IIIa, did not interact with melanoma cells, indicating that the platelet GPIIb-IIIa complex is also necessary for the platelet-melanoma cell interaction. This work demonstrates the importance of the IIb-IIIa-like GPs, present on M3Dau melanoma cells, in mediating tumor-platelet interactions.
Boukerche
H
H
Faculté de Médicine Alexis Carrel, INSERM Unite 63, Lyon, France.
Berthier-Vergnes
O
O
Tabone
E
E
Doré
J F
JF
Leung
L L
LL
McGregor
J L
JL
D000911:Antibodies, Monoclonal / Q000502:physiology; D001667:Binding, Competitive; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D002450:Cell Communication; D006801:Humans; D008545:Melanoma / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000276:immunology / Q000502:physiology*; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D010980:Platelet Membrane Glycoproteins