Perinatal lethality (ple): a mutation caused by integration of a transgene into distal mouse chromosome 15.
We have used cytogenetic and recombinational analysis to determine the position of a transgene integrated into the mouse genome. The transgene maps to band F on the physical map of mouse chromosome 15 by in situ analysis and is tightly linked genetically to a cluster of loci that include the mutations caracul (Ca) and microcytic anemia (mk). Genetic analysis of the offspring of noninbred animals carrying the transgene and marker loci demonstrates a significant deficiency of homozygous progeny at weaning. When inbred mice heterozygous for the transgene are mated, about one-quarter of their offspring are homozygous; none of these animals survives more than 1 day after birth. It appears likely that a recessive insertional mutation has occurred as a result of transgene integration into a locus required for postnatal viability. We call this mutation transgenic perinatal lethality (Tg.ple).
Beier
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DR
Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts 02115.
Morton
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CC
Leder
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A
Wallace
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R
Leder
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P
D000818:Animals; D002874:Chromosome Mapping; D005804:Genes, Lethal; D005808:Genes, Recessive; D005813:Genes, Synthetic; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D008822:Mice, Transgenic / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011995:Recombination, Genetic