title: Purification of acidic fibroblast growth factor from bovine omentum.
abstract: Two heparin binding growth factors with different molecular weight, 16.6 kD and 18.6 kD polypeptide, were purified from bovine omentum. The two factors have almost the same affinity to heparin; they were eluted with 1.0 M NaCl from the affinity column. The 16.6 kD polypeptide was found to be acidic fibroblast growth factor by amino acid sequence analysis. The 18.6 kD polypeptide was an N-terminus blocked polypeptide and was suggested to be beta-endothelial cell growth factor. These molecular species may play significant roles in maintaining vascularized structure in omentum and be related to the angiogenic activity of the tissue.
authors.affiliation: Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohtaki
authors.forename: K
authors.initials: K
authors.lastname: Wakamatsu
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: T
authors.initials: T
authors.lastname: Yasuhara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D005346:Fibroblast Growth Factors / Q000302:isolation & purification* / Q000502:physiology; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009852:Omentum / Q000032:analysis*
chemical_list: D005346:Fibroblast Growth Factors; D004247:DNA