Identification of in vitro metabolites of 3-ethyl-2,6-dimethyl-4H-pyrido(1,2a)pyrimidin-4-one.
(2-14C)3-Ethyl-2,6-dimethyl-4H-pyrido(1,2a)pyrimidin-4-one is metabolized in vitro by liver microsomes to at least 12 metabolites. The metabolites were isolated and purified by thin-layer chromatography and high-performance liquid chromatography and identified by mass spectrometry and nuclear magnetic resonance spectroscopy. The mostly mono- and dihydroxylated isomers were distinguished on the bases of their electron ionization mass spectra and the metastable daughter ion spectra of selected ions.
Jemnitz
K
K
Central Research Institute for Chemistry of the Hungarian Academy of Sciences, Budapest.
Dénes
G
G
Tamás
J
J
Gács-Baitz
E
E
D000700:Analgesics / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D002855:Chromatography, Thin Layer; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D008970:Molecular Weight; D011744:Pyrimidinones / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000700:Analgesics; D011744:Pyrimidinones; C045845:CH 150