Endogenous digoxin-like material in normotensive and hypertensive strains of rats.
We measured the concentration of endogenous digoxin-like materials (EDLM) in the serum of spontaneously hypertensive rats (SHR) and three normotensive rat strains at four stages during growth using a sensitive RIA. In the SHR, there was a significant peak in the EDLM level between 0.057-0.087 ngE/mL at 6 to 8 weeks of age, shortly after the onset of hypertension. The EDLM concentration returned to normal levels by 20 weeks of age. Sprague-Dawley and Wistar-Kyoto rats had EDLM levels below 0.050 ngE/mL at all time points studied. In contrast, Fischer 344 rats displayed persistently elevated serum EDLM concentrations that exceeded 0.124 ngE/mL from 3 to 20 weeks of life. We conclude that (1) there are significant interstrain differences in serum EDLM levels in rats; and (2) the SHR has a unique peak in serum EDLM levels at 6 to 8 weeks of age, indicating a possible role for the substance in the inception of hypertension.
Trachtman
H
H
Department of Pediatrics, Schneider Children's Hospital, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
Delpizzo
R
R
Chasalow
F I
FI
D000367:Age Factors; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D001835:Body Weight; D002298:Cardenolides; D004077:Digoxin; D006973:Hypertension / Q000097:blood*; D051381:Rats; D011916:Rats, Inbred F344 / Q000097:blood; D011918:Rats, Inbred SHR / Q000097:blood*; D011919:Rats, Inbred Strains / Q000097:blood*; D011921:Rats, Inbred WKY / Q000097:blood; D012503:Saponins
D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D004077:Digoxin