Excimer fluorescence in lens proteins labelled with N-(1-pyrene)maleimide.
Lens proteins labelled with the sulphydryl reagent, N-(1-pyrene)maleimide exhibit pyrene excimer fluorescence around 470 nm. This was found to be associated with both the beta- and gamma-crystallins but not the alpha-crystallins. Disappearance of the 470 nm peak on exposure to denaturants indicates it arose from an intramolecular excimer. This excimer fluorescence provides a new and sensitive tool for monitoring conformational changes in the beta- and gamma-crystallins.
Putilina
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T
Russell Grimwade School of Biochemistry, Melbourne University, Victoria, Australia.
Augusteyn
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RC
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D003459:Crystallins / Q000378:metabolism*; D005453:Fluorescence; D008301:Maleimides / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D003459:Crystallins; D008301:Maleimides; C003944:N-(3-pyrene)maleimide