[In vitro and in vivo chemosensitivity of the YTS-1 human bladder carcinoma cell line].
In vitro and in vivo cytotoxicity tests were carried out using the YTS-1 human bladder carcinoma cell line. The lethal effects of a 1-hour treatment with 5 anti-cancer agents (Cisplatin [CDDP], Cytosine arabinoside [ara-C], Adriamycin [ADM], Etoposide [VP-16] and Carboplatin [CBDCA]) on the YTS-1 cells grown as monolayer were examined quantitatively by the regrowth assay methods. In addition, the lethal effects of 3 anti-cancer agents (CDDP, ADM, ara-C) were also examined in vivo. Moreover the effects of simultaneous combinations of CDDP and ara-C, and ADM and ara-C were assessed in vitro and in vivo. In vitro studies showed that, of these single agents, ADM and ara-C were cytotoxic judging from the human maximal plasma concentration of drugs, whereas CDDP, VP-16 and CBDCA were not. Combinations of CDDP and ara-C, and ADM and ara-C showed more than additive effect. In vivo studies on YTS-1 tumors implanted to athymic nude mice (BALB/c) showed that ADM was the cytotoxic agent. Although CDDP alone or ara-C alone was not cytotoxic to YTS-1 tumors, combination of CDDP and ara-C showed antitumor activity. These results show that combination of CDDP and ara-C is synergistic. The most effective combination was observed to be ADM and ara-C, but severe loss of body weight was also observed.
Kakizaki
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D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D051379:Mice; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D000970:Antineoplastic Agents