Induction of tumour hypoxia by a vasoactive agent. A combined NMR and radiobiological study.
The effect of hydralazine treatment on 3 murine tumours (RIF-1, KHT and 16/C) was monitored using 31P-NMR. Changes in the 31P-NMR spectrum are compared with measurements of radiobiological hypoxic fraction (RHF) in the RIF-1 and KHT. Hydralazine is known to reduce temporarily blood flow in experimental tumours, and thus cause a transient increase in the RHF to 100% (in RIF-1 and KHT). This correlates with a decline in energy status as measured by 31P-NMR (i.e. there was an increase in Pi in all three tumours). Time-course data from the RIF-1 and KHT tumours show that maintenance of anaesthesia prolongs the hypoxia induced by hydralazine.
Dunn
J F
JF
MRC Biochemical & Clinical Magnetic Resonance Unit, Department of Biochemistry, University of Oxford, England.
Frostick
S
S
Adams
G E
GE
Stratford
I J
IJ
Howells
N
N
Hogan
G
G
Radda
G K
GK
D000818:Animals; D006830:Hydralazine / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000098:blood supply / Q000378:metabolism* / Q000532:radiotherapy; D010100:Oxygen / Q000378:metabolism*; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D014662:Vasoconstrictor Agents; D006830:Hydralazine; D010100:Oxygen