title: Antibodies to hnRNP core protein A1 in connective tissue diseases.
abstract: We investigated the specificity of circulating autoantibodies to a heterogeneous nuclear ribonuclear protein A1 (hnRNP A1), obtained by recombinant DNA technique, in different rheumatic diseases: systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), scleroderma, primary Sjogren's syndrome (SS), idiopathic Raynaud (IR), mixed connective tissue disease (MCTD), and healthy donors. All sera were tested by ELISA on hnRNP A1 protein. Positive values were obtained in 22% SLE, 19% scleroderma, 10% IR, 40% (2/5) MCTD, 5% SS, and 50% RA patients. The majority of patients reacted with the aminoterminal part (UP1) of hnRNP A1; however, some RA patients reacted also with the carboxy-terminal part that shows partial homology with keratin. Therefore, hnRNP A1 (UP1) can be considered a target of antinuclear autoimmunity in various rheumatic disorders.
authors.affiliation: Istituto di Genetica Biochimica ed Evoluzionistica del C.N.R., Pavia, Italy.
authors.forename: G C
authors.initials: GC
authors.lastname: Astaldi Ricotti
authors.forename: M
authors.initials: M
authors.lastname: Bestagno
authors.forename: A
authors.initials: A
authors.lastname: Cerino
authors.forename: C
authors.initials: C
authors.lastname: Negri
authors.forename: R
authors.initials: R
authors.lastname: Caporali
authors.forename: F
authors.initials: F
authors.lastname: Cobianchi
authors.forename: M
authors.initials: M
authors.lastname: Longhi
authors.forename: C
authors.initials: C
authors.lastname: Maurizio Montecucco
mesh_terms: D001323:Autoantibodies / Q000032:analysis*; D001798:Blood Proteins / Q000378:metabolism; D015153:Blotting, Western; D003240:Connective Tissue Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D011485:Protein Binding; D012261:Ribonucleoproteins / Q000276:immunology* / Q000378:metabolism
chemical_list: D001323:Autoantibodies; D001798:Blood Proteins; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012261:Ribonucleoproteins; C000619656:hnRNPA1 protein, human