An automated high-performance liquid chromatographic trace enrichment method for the determination of metoclopramide in serum and its application to a bioequivalence human volunteer study.
An automated trace enrichment method for metoclopramide is described. Serum was injected on to a short column packed with PLRP-S 100A (a polymeric reversed-phase material). The unwanted components were washed off with borate buffer (pH 9.8) before switching to a Spherisorb ODS column for the separation. The limit of detection in serum samples was 2 ng/ml. The method was used in a pharmacokinetic study to compare the biovailability of two formulations of the drug.
Fairhead
A P
AP
Department of Clinical Chemistry, Rorer Health Care Limited, Eastbourne, East Sussex.
Brooks
S G
SG
Butterworth
K R
KR
Mangham
B A
BA
D000328:Adult; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008787:Metoclopramide / Q000097:blood* / Q000493:pharmacokinetics; D011237:Predictive Value of Tests
D008787:Metoclopramide