Fluorometric measurement of ofloxacin uptake by human polymorphonuclear leukocytes.
A fluorometric assay, based on the natural fluorescence of the quinolone nucleus, was used to determine the uptake of ofloxacin by human polymorphonuclear leukocytes. The ratio of cellular concentration to extracellular concentration (C/E) at 20 min and 37 degrees C was 7.2, using an extracellular concentration of 5 micrograms/ml. Uptake was rapid and was not affected by pH (5 to 9), but required elevated environmental temperature and cell viability. The metabolic inhibitors sodium fluoride and sodium cyanide significantly decreased the uptake of ofloxacin. The penetration of ofloxacin was not affected by the presence of glucose or adenosine, but was decreased by L-amino acids (lysine, leucine, and glycine). These results suggest that ofloxacin could be transported via an amino acid transport system and that the fluorometric assay is a useful method for determining the intracellular penetration of fluoroquinolones, avoiding the use of radiolabeled antimicrobial agents.
Pascual
A
A
Department of Microbiology, School of Medicine, University of Seville, Spain.
Garcia
I
I
Perea
E J
EJ
D000241:Adenosine / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000032:analysis / Q000378:metabolism*; D015242:Ofloxacin / Q000097:blood*; D013050:Spectrometry, Fluorescence; D013696:Temperature
D000596:Amino Acids; D015242:Ofloxacin; D005947:Glucose; D000241:Adenosine