Detection of organophosphate detoxifying esterases by dot-blot immunoassay in Culex mosquitoes.
A dot-blot immunoassay, using antiserum raised against esterase B1 responsible for organophosphate (OP) resistance in Culex quinquefasciatus, was used to study different laboratory strains and field collections of this species, as well as of Cx. pipiens, Cx. tarsalis, Anopheles albimanus and An. stephensi. The frequency of esterase B1 positive individuals revealed by this test was in agreement with the data obtained by other methods, indicating the potential of this method for the detection and monitoring of resistance due to increased detoxification by esterases.
Beyssat-Arnaouty
V
V
Institut des Sciences de l'Evolution (CNRS, UA 327), Université de Montpellier II, France.
Mouches
C
C
Georghiou
G P
GP
Pasteur
N
N
D000818:Animals; D000852:Anopheles / Q000201:enzymology; D003465:Culex / Q000201:enzymology*; D005260:Female; D015151:Immunoblotting; D007305:Insecticide Resistance; D007306:Insecticides / Q000378:metabolism*; D008297:Male; D009943:Organophosphorus Compounds; D012697:Serine Endopeptidases / Q000032:analysis*; D013045:Species Specificity
D007306:Insecticides; D009943:Organophosphorus Compounds; D012697:Serine Endopeptidases; C049262:esterase B