title: Effects of gastrointestinal hormones on the growth of human intestinal epithelial cells in vitro.
abstract: The growth of cultured epithelium like cells from human normal embryonic intestine was studied in response to various hormones using a method that quantifies the number of cells by the amount of dye that they bind after fixation. Gastrin and neurotensin in the pg/ml range and higher caused small increases in cell growth. Glucagon and VIP were stimulatory in the low ng/ml range, whereas somatostatin and bombesin had no effect at the lower concentrations but were stimulatory at the highest concentration tested (10 and 100 ng/ml respectively). Secretin and pancreozymin (cholecystokinin) seemed to be ineffective.
authors.affiliation: Department of Surgery, King's College School of Medicine and Dentistry, London.
authors.forename: C
authors.initials: C
authors.lastname: Simopoulos
authors.forename: J D
authors.initials: JD
authors.lastname: Gaffen
authors.forename: A
authors.initials: A
authors.lastname: Bennett
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005755:Gastrins / Q000494:pharmacology; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects*
chemical_list: D005755:Gastrins; D005768:Gastrointestinal Hormones