Isolation and mapping of DNA probes within the linkage group I gene cluster of Caenorhabditis elegans.
We have isolated probes for DNA polymorphisms across the linkage group I gene cluster in Caenorhabditis elegans, using Tc1-linkage selection. The probes detect strain polymorphism between the wild-type strains of var. Bristol and var. Bergerac. As a result of mapping the sites hP4, hP5, hP6, hP7, hP9, and sPl, more than 1000 kilobases (kb) of cloned cosmid DNA has been positioned on the genetic map. We found there is more DNA per map unit in the center of the gene cluster than expected on the basis of the genomic average. Furthermore, the amount is not constant across the entire region but reaches a peak in the hP9 unc-13 interval. To find the coding regions, we examined DNA cross-homology between two species, Caenorhabditis elegans and Caenorhabditis briggsae. Approximately one-third of the DNA in the hP5 hP9 interval was examined for coding regions and 21 sequences were identified within 318 kb of DNA.
Starr
T
T
Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
Howell
A M
AM
McDowall
J
J
Peters
K
K
Rose
A M
AM
D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes / Q000302:isolation & purification*; D008040:Genetic Linkage; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D015183:Restriction Mapping
D015342:DNA Probes; D004247:DNA