title: Laboratory characterization of a swine influenza virus isolated from a fatal case of human influenza.
abstract: A swine influenza virus-like type A (H1N1) virus, designated A/Wisconsin/3523/88, was isolated in September 1988 from a Wisconsin woman who had died with primary viral pneumonia. Antigenic analyses with hemagglutinin-specific monoclonal antibodies and postinfection ferret serum indicated that the hemagglutinin of A/Wisconsin/3523/88 was antigenically closely related to viruses currently circulating in swine. Genetic analysis of the A/Wisconsin/3523/88 virus by RNA fingerprinting and partial RNA sequence analysis of seven of the eight segments indicated that the genome of the human isolate was similar to that of enzootic swine viruses. These laboratory data supported the epidemiologic findings that this human infection occurred by transmission of an enzootic swine influenza virus and that the virus showed no major genetic changes potentially related to increased pathogenesis.
authors.affiliation: Division of Viral Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
authors.forename: P A
authors.initials: PA
authors.lastname: Rota
authors.forename: E P
authors.initials: EP
authors.lastname: Rocha
authors.forename: M W
authors.initials: MW
authors.lastname: Harmon
authors.forename: V S
authors.initials: VS
authors.lastname: Hinshaw
authors.forename: M G
authors.initials: MG
authors.lastname: Sheerar
authors.forename: Y
authors.initials: Y
authors.lastname: Kawaoka
authors.forename: N J
authors.initials: NJ
authors.lastname: Cox
authors.forename: T F
authors.initials: TF
authors.lastname: Smith
mesh_terms: D000328:Adult; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007251:Influenza, Human / Q000382:microbiology*; D009710:Nucleotide Mapping; D011024:Pneumonia, Viral / Q000382:microbiology*; D012367:RNA, Viral / Q000032:analysis*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral