title: Catalytic properties of thermophilic lactate dehydrogenase and halophilic malate dehydrogenase at high temperature and low water activity.
abstract: Thermophilic lactate dehydrogenases from Thermotoga maritima and Bacillus stearothermophilus are stable up to temperature limits close to the optimum growth temperature of their parent organisms. Their catalytic properties are anomalous in that Km shows a drastic increase with increasing temperature. At low temperatures, the effect levels off. Extreme halophilic malate dehydrogenase from Halobacterium marismortui exhibits a similar anomaly. Increasing salt concentration (NaCl) leads to an optimum curve for Km, oxaloacctate while Km, NADH remains constant. Previous claims that the activity of halophilic malate dehydrogenase shows a maximum at 1.25 M NaCl are caused by limiting substrate concentration; at substrate saturation, specific activity of halophilic malate dehydrogenase reaches a constant value at ionic strengths I greater than or equal to 1 M. Non-halophilic (mitochondrial) malate dehydrogenase shows Km characteristics similar to those observed for the halophilic enzyme. The drastic decrease in specific activity of the mitochondrial enzyme at elevated salt concentrations is caused by the salt-induced increase in rigidity of the enzyme, rather than gross structural changes.
authors.affiliation: Institut für Biophysik und Physikalische Biochemie, Universität Regensburg, Federal Republic of Germany.
authors.forename: K
authors.initials: K
authors.lastname: Hecht
authors.forename: A
authors.initials: A
authors.lastname: Wrba
authors.forename: R
authors.initials: R
authors.lastname: Jaenicke
mesh_terms: D002384:Catalysis; D004789:Enzyme Activation; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology*; D006217:Halobacterium / Q000201:enzymology*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014867:Water
chemical_list: D014867:Water; D012965:Sodium Chloride; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase