Changes in inducible nuclear factors with specific binding ability to c-myc gene fragments in peripheral blood mononuclear cells from systemic lupus erythematosus patients.
Using a gel retardation assay, we found new nuclear proteins of peripheral blood mononuclear cells (PBMC) from systemic lupus erythematosus (SLE) patients specifically binding to c-myc gene fragments. A different pattern could be detected between SLE patients and healthy controls for the c-myc exon I fragment, but both groups had similar patterns for the c-myc 5'-upper region fragment. For the c-myc exon I fragment, one DNA-protein complex was induced only in SLE patients and the other DNA-protein complex was amplified in SLE and normal PBMC with mitogen stimulation. It is suggested that abnormal c-myc protooncogene transcription in PBMC from SLE patients could be related to differences in nuclear specific binding proteins. These differences apparently play a key role in abnormal lymphocyte function in changes in gene regulation.
Deguchi
Y
Y
Third Department of Internal Medicine, Osaka University School of Medicine, Japan.
Negoro
S
S
Kishimoto
S
S
D004247:DNA / Q000097:blood / Q000235:genetics; D004268:DNA-Binding Proteins / Q000097:blood; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000235:genetics; D009687:Nuclear Proteins / Q000097:blood*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D004247:DNA