Use of monoclonal antibodies in the purification of an inhibitor of virus replication by affinity chromatography.
Mouse monoclonal antibodies (MAbs) were prepared to an inhibitor of virus replication (IVR), released from protoplasts or leaf tissue of hypersensitive tobacco plants infected with tobacco mosaic virus. The MAbs were highly specific for IVR and reduced its antiviral activity. Using these MAbs in affinity chromatography enabled the recovery of purified IVR. SDS-PAGE of the immunoaffinity-purified IVR gave a single Mr 23K band. Immunoblots of IVR from extracts of protoplast or leaf tissue also revealed a single Mr 23K band which suggests that protoplast and tissue IVR are closely related.
Gera
A
A
Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Sadka
A
A
Spiegel
S
S
Salomon
R
R
Smorodinsky
N I
NI
D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity; D000998:Antiviral Agents / Q000032:analysis / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D010947:Plants, Toxic; D011523:Protoplasts / Q000382:microbiology; D014026:Nicotiana / Q000382:microbiology; D014027:Tobacco Mosaic Virus / Q000502:physiology*; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D000998:Antiviral Agents