title: Plasma esterase-1 (ES-1) activity is increased in rats fed high-fat diets.
abstract: The question addressed is whether the amount and type of dietary fat affects esterases in plasma. Rats were fed semipurified diets containing 2.0 to 19.4% (w/w) of fat in the form of coconut fat or corn oil. Fat was added to the diets at the expense of isocaloric amounts of carbohydrates. Plasma total esterase activities measured with 4-nitrophenylacetate as substrate were slightly increased with increasing fat intakes. However, an increase in fat concentration of the diet was associated with a pronounced increase in the activity of the so-called ES-1 isozyme in plasma. ES-1, which represents very little plasma total esterase activity, was quantified densitometrically as the high-mobility, anodal esterase band on polyacrylamide gel electrophoresis. The positive association between amount of dietary fat and ES-1 activity was identical for coconut fat and corn oil.
authors.affiliation: Department of Laboratory Animal Science, State University, Utrecht, The Netherlands.
authors.forename: H A
authors.initials: HA
authors.lastname: Van Lith
authors.forename: G W
authors.initials: GW
authors.lastname: Meijer
authors.forename: L F
authors.initials: LF
authors.lastname: Van Zutphen
authors.forename: A C
authors.initials: AC
authors.lastname: Beynen
mesh_terms: D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D000074263:Coconut Oil; D003314:Corn Oil / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D008297:Male; D010938:Plant Oils; D051381:Rats
chemical_list: D004041:Dietary Fats; D010938:Plant Oils; D003314:Corn Oil; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D000074263:Coconut Oil