A subset of human rosetted lymphocytes contains previously unidentified histamine.
By sequential solid-phase immunoadsorption (panning) steps, we have isolated a subset of lymphocytes (comprising 3-7% of rosetted cells) that contains high concentrations of histamine. We have used a radioenzymatic assay for the determination of histamine and have located 117 ng of histamine/1 x 10(6) cells in Leu-5+ (OKT-11), Leu-15+ cells. This subset did not contain basophils and was negative for Leu-4 (OKT-3), Leu-3 (OKT-4), Leu-2 (OKT-8), and 9.3 antigens. The function of this subset of rosetted cells has not been determined.
Khan
M M
MM
Department of Medicine, Stanford University School of Medicine, California 94305.
Fishwild
D M
DM
Melmon
K L
KL
Coutre
S
S
Engleman
E G
EG
Bauer
J A
JA
D000943:Antigens, Differentiation / Q000032:analysis; D006632:Histamine / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000145:classification; D012397:Rosette Formation
D000943:Antigens, Differentiation; D006632:Histamine