Azido derivatives of dicarboxylic acids for photoaffinity labeling of mitochondrial carriers.
New photoaffinity probes, N-(4-azidosalicylic)-aminosuccinic acid, 3-(4-azidophenylazo)-4-hydroxyphenylmalonic acid, (4-azido-2-nitroanilino)-N-succinic acid, 4-azidophenacylthiosuccinic acid and 4-azidophenylsuccinic acid, were synthesized and characterized chemically. They differ in the distance between dicarboxylic and azido groups, hydrophobicity and acidic moiety. These between dicarboxylic and azido groups, hydrophobicity and acidic moiety. These reagents can be applied for photoaffinity labeling of mitochondrial anion carriers and enzymes interacting with dicarboxylic acids. Inhibition and labeling of the dicarboxylate carrier is presented.
Szewczyk
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Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
Nałecz
M J
MJ
Wojtczak
L
L
D000345:Affinity Labels / Q000138:chemical synthesis / Q000378:metabolism*; D000818:Animals; D001386:Azides / Q000378:metabolism; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003998:Dicarboxylic Acids / Q000138:chemical synthesis / Q000378:metabolism*; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism*; D010782:Photolysis; D051381:Rats
D000345:Affinity Labels; D001386:Azides; D002352:Carrier Proteins; D003998:Dicarboxylic Acids