Micronucleus test with 6-mercaptopurine monohydrate administered intraperitoneally and orally.
The effect of route of administration on the induction of micronucleated polychromatic erythrocytes (MNPCEs) was examined. 6-Mercaptopurine monohydrate (6-MP) was administered intraperitoneally (i.p.) or orally (p.o.) to 2 strains of mice, MS/Ae and CD-1. From the results of an acute toxicity test and a pilot micronucleus test, the doses selected for the final micronucleus test were 12.5-100 mg/kg for the i.p. route and 25-200 mg/kg for the p.o. route. The sampling time was 48 h. Frequencies of MNPCEs increased dose-dependently by the i.p. route but peaked at 50 or 100 mg/kg for the p.o. route. 6-MP induced MNPCEs more efficiently after p.o. administration than after i.p. treatment in both strains.
Hara
T
T
Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
Makita
T
T
Horiya
N
N
Ozawa
S
S
Ohba
M
M
Naito
J
J
Shibuya
T
T
D000284:Administration, Oral; D000818:Animals; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D015122:Mercaptopurine / Q000008:administration & dosage* / Q000633:toxicity; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage*
D009153:Mutagens; D015122:Mercaptopurine