title: [Cytospectrophotometric research on hemoglobin in human erythrocytes. I. The methemoglobin content in intact erythrocytes and its alteration under the influence of chromosmon, ascorbic acid, riboflavin and glutathione].
abstract: The blood of healthy men and patients with methemoglobinemia of different genesis was incubated with chromosmon, ascorbic acid, riboflavin and glutathione, the percentage of erythrocytes with thorn-shaped protuberances-echinocytes being subsequently determined in the blood smears. The absorbtion spectra at the range 400-650 nm were investigated both in the smooth erythrocytes and in echinocytes. A correlation was found between the percentage of echinocytes and the methemoglobin content in the blood. The methemoglobin amount in the echinocytes was determined to be higher than in the smooth erythrocytes. It is discovered that effects of chromosmon, glutathione and riboflavin on production of methemoglobin depend on the dose, individual peculiarities of erythrocytes and on the illness that caused methemoglobinemia. The calculation of echinocyte percentage may be used as an express-diagnostics of methemoglobinemia and for purposes of studying the effect of methemoglobin-producing substances and drugs.
authors.forename: L V
authors.initials: LV
authors.lastname: Filev
authors.forename: I I
authors.initials: II
authors.lastname: Zakharov
authors.forename: G V
authors.initials: GV
authors.lastname: Selivanova
authors.forename: E B
authors.initials: EB
authors.lastname: Zhiburt
authors.forename: V A
authors.initials: VA
authors.lastname: Kuznetsov
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology*; D001991:Bronchitis / Q000097:blood / Q000150:complications; D002908:Chronic Disease; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000032:analysis* / Q000187:drug effects; D005978:Glutathione / Q000494:pharmacology*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008706:Methemoglobin / Q000032:analysis*; D008708:Methemoglobinemia / Q000097:blood* / Q000209:etiology; D008751:Methylene Blue / Q000494:pharmacology*; D011660:Pulmonary Heart Disease / Q000097:blood / Q000150:complications; D012256:Riboflavin / Q000494:pharmacology*; D018805:Sepsis / Q000097:blood / Q000150:complications; D012774:Shock, Traumatic / Q000097:blood / Q000150:complications; D013053:Spectrophotometry
chemical_list: D006454:Hemoglobins; D008706:Methemoglobin; D005978:Glutathione; D001205:Ascorbic Acid; D008751:Methylene Blue; D012256:Riboflavin
vernacular_title: Tsitospektrofotometricheskoe issledovanie gemoglobina v éritrotsitakh cheloveka. I. Soderzhanie metgemoglobina v intaktnykh éritrotsitakh i ego izmenenie pod vliianiem khromosmona, askorbinovoĭ kisloty, riboflavina i gliutationa.