Application of sorbent extraction chromatography to the purification of diethylstilboestrol extracted from muscle tissue and determined by radioimmunoassay.
A chromatographic procedure is described for the purification of bovine muscle tissue extracts prior to the determination of diethylstilboestrol (DES) by radioimmunoassay. Sorbent extraction chromatography of tissue extracts on reversed-phase octadecyl (C18) columns gives adequate recovery of residue and a suitable sample for radioimmunoassay. This procedure, which is simple and rapid, provides an alternative to a more complex purification by high-performance liquid chromatography. Using this method, the limit of detection for DES in muscle samples is approximately 40 pg/g.
O'Keeffe
M
M
National Food Centre, Teagasc, Dunsinea, Castleknock, Dublin, Ireland.
Hopkins
J P
JP
D000818:Animals; D002417:Cattle; D002845:Chromatography; D004054:Diethylstilbestrol / Q000032:analysis* / Q000302:isolation & purification; D005260:Female; D008297:Male; D009132:Muscles / Q000032:analysis*; D011863:Radioimmunoassay
D004054:Diethylstilbestrol