title: [Reactivity of oligonucleotide derivatives, containing a methylphosphate group. Affinity modification of target DNA by 4-N-(methyl-N-2-chlorethylamino)benzyl 3'- and 5'-phosphamide derivatives of octathymidylate containing methylphosphonate residues].
abstract: Modification of 5'-32P-labelled octadecadeoxyribonucleotide d(pC5A8C5) (III) with octathymidylate methylphosphonate derivatives bearing both 3'- and 5'-terminal alkylating 4-(N-2-chloroethyl-N-methylamino)benzylphosphoamide residue has been investigated. Yield in the modification depends on configuration of methylphosphonate fragment, in case of Rp-isomer it may amount to 90%. Specificity of alkylation of nucleic acide target (III) by reagents based on the oligonucleotide methylphosphonates is almost the same as by reagents based on the oligonucleotides having phosphodiester internucleotide bonds.
authors.forename: N V
authors.initials: NV
authors.lastname: Amirkhanov
authors.forename: V F
authors.initials: VF
authors.lastname: Zarytova
mesh_terms: D000345:Affinity Labels; D001483:Base Sequence; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009588:Nitrogen Mustard Compounds; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D013942:Thymine Nucleotides
chemical_list: D000345:Affinity Labels; D009588:Nitrogen Mustard Compounds; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D013942:Thymine Nucleotides; D004247:DNA
vernacular_title: Reaktsionnosposobnye proizvodnye oligonukleotidov, soderzhashchikh metilfosfatnye grupy. Affinnaia modifikatsiia DNA-misheni 4-(N-metil-N-2-khlorétilamino)benzil 3'- i 5'-fosfamidnymi proizvodnymi oktatimidilata, soderzhashchimi metilfosfonatnye ostatki.