Cerebral interferon entry in mice after osmotic alteration of blood-brain barrier.
Systemically administered interferon (IFN) is not readily detected in the central nervous system (CNS) due to the presence of the blood-brain barrier (BBB). A method of osmotic BBB alteration in a mouse model was established in this laboratory. IFN's entry into the normal and osmotically altered brain after its intracarotid injection was investigated. Significant IFN levels (100-1,000 units) in the brain can be achieved by this method. The highest IFN activity was found in the brain hemisphere ipsilateral to the injection site within 20 min to 1 h after injection. IFN activity in the brain was detectable up to 4 h. Animals injected in this manner with murine IFN-alpha/beta (MuIFN-alpha/beta) and observed for a 6-month time interval showed no signs of neurological dysfunctions and resumed their normal activities. The therapeutic value of this method will be tested in a murine model of malignant glioma.
Wiranowska
M
M
Department of Neurology, College of Medicine, University of South Florida, Tampa 33612.
Wilson
T C
TC
Thompson
K
K
Prockop
L D
LD
D000818:Animals; D001089:Arabinose / Q000008:administration & dosage; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D007263:Infusions, Parenteral; D007370:Interferon Type I / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size; D009995:Osmosis / Q000187:drug effects; D013997:Time Factors
D007370:Interferon Type I; D001089:Arabinose