title: Lymphocyte proliferative response to mitogenic monoclonal antibodies in systemic sclerosis. Evidence for unresponsiveness to murine monoclonal antibodies of IgG1 isotype.
abstract: Proliferative response of peripheral blood mononuclear cells to phitohemoagglutinin and anti-CD3 mitogenic monoclonal antibodies (MoAbs) of the IgG2a (OKT3) and IgG1 (PanT2, CLB T3/4.1) isotypes was studied in 39 patients with systemic sclerosis (SSc) and in 82 control subjects. The effect of IL-2 on this response was also investigated. No difference in the response to PHA and to IgG2a anti-CD3 MoAb OKT3 was seen between scleroderma patients and controls. Both the patient and control groups contained responders and non-responders to IgG1 anti-CD3 MoAbs. The percentage of non-responders was significantly higher in scleroderma patients than in controls. When purified lymphocytes from non-responder scleroderma patients were cultured with monocytes from control responders, proliferative response to IgG1 MoAbs was restored. Our results show that monocytes from patients with systemic sclerosis bear a defect leading to IgG1 unresponsiveness by T lymphocytes.
authors.affiliation: Clinica Medica, I Facoltà di Medicina e Chirurgia, Napoli, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Valentini
authors.forename: P
authors.initials: P
authors.lastname: Carandente Giarrusso
authors.forename: L
authors.initials: L
authors.lastname: Gualdieri
authors.forename: L
authors.initials: L
authors.lastname: Corbo
authors.forename: F
authors.initials: F
authors.lastname: Alfinito
authors.forename: M C
authors.initials: MC
authors.lastname: Turco
authors.forename: S
authors.initials: S
authors.lastname: Venuta
authors.forename: M
authors.initials: M
authors.lastname: Giordano
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005260:Female; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008875:Middle Aged; D009000:Monocytes / Q000473:pathology; D012595:Scleroderma, Systemic / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes