Involvement of protein kinase C in the histamine H1-receptor mediated contraction of guinea-pig lung parenchymal strips.
In this study we investigated the role of protein kinase C (PKC) in the histamine H1-receptor mediated contraction of guinea-pig parenchymal lung strips. Lung strips contract after administration of the PKC activators phorbol-12-myristate-13-acetate (PMA) and dioctanoylglycerol. Prolonged stimulation (45 min.) with a high concentration of PMA abolished subsequent responses to PMA whereas H1-responses were only partially reduced. Administration of a PKC inhibitor (H-7) also led to an inhibition of H1-responses. Experiments with combined incubations with the PKC inhibitor H-7 and the Ca2+-entry blocker verapamil suggest that the PKC contribution to the response is possibly mediated via regulation of Ca2+ influx.
Leurs
R
R
Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
Go
J N
JN
Bast
A
A
Timmerman
H
H
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D011725:Pyridines / Q000494:pharmacology; D011969:Receptors, Histamine H1 / Q000187:drug effects / Q000502:physiology*
D007546:Isoquinolines; D010879:Piperazines; D011725:Pyridines; D011969:Receptors, Histamine H1; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C006183:2-(2-aminoethyl)pyridine; D011493:Protein Kinase C