title: Ingestion of immune bloodmeals and infection of Aedes fowleri, Aedes mcintoshi, and Culex pipiens with Rift Valley fever virus.
abstract: Rift Valley fever (RVF) virus infection, dissemination, and transmission rates were determined for Aedes fowleri, Aedes mcintoshi and Culex pipiens 7 or 10 days after sequentially feeding to repletion on RVF virus immune hamsters and RVF viremic hamsters, or after feeding on a mixture of RVF virus immune sheep serum and RVF viremic hamster blood through a pledget. No significant differences in infection or dissemination rates were detected among Ae. fowleri and Cx. pipiens feeding to repletion on immune hamsters before or after feeding to repletion on a viremic hamster. Similarly, no significant differences in infection, dissemination, or transmission rates were observed among Ae. fowleri and Cx. pipiens feeding to repletion on immune hamsters or nonimmune (control) hamsters 0 or 24 hr after inoculation with RVF virus. Infection rates were significantly higher for Ae. fowleri (56/66, 85%) and Cx. pipiens (123/148, 83%) fed only on viremic hamsters than for those interrupted to complete feeding on an immune hamster (Ae. fowleri [24/49, 59%], Cx. pipiens [66/131, 50%]) or a nonimmune hamster (Ae. fowleri [32/51, 63%], Cx. pipiens [69/127, 54%]). However, no significant differences were detected in infection, dissemination, or transmission rates among Ae. fowleri, Ae. mcintoshi or Cx. pipiens fed on a viremic hamster and interrupted to complete feeding on an immune vs. a nonimmune hamster. Results from interrupted feeding experiments were significantly different from pledget feeding experiments.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland.
authors.forename: L A
authors.initials: LA
authors.lastname: Patrican
authors.forename: C L
authors.initials: CL
authors.lastname: Bailey
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D002043:Bunyaviridae / Q000276:immunology*; D006224:Cricetinae; D003465:Culex / Q000382:microbiology*; D005260:Female; D007106:Immune Sera / Q000276:immunology*; D007303:Insect Vectors / Q000382:microbiology*; D012295:Rift Valley Fever / Q000276:immunology / Q000635:transmission; D012296:Rift Valley fever virus / Q000276:immunology* / Q000502:physiology; D014766:Viremia / Q000276:immunology
chemical_list: D007106:Immune Sera