Effects of progesterone and estradiol on aminopeptidase A (AAP) and leucine aminopeptidase (LAP) activities in the pregnancy serum and placenta of the rat.
We investigated the effects of estradiol and progesterone on placental aminopeptidase A and leucine aminopeptidase activities in pregnant rats. Estradiol (0.5, 1.0 and 2 mg/day) plus progesterone (5, 10 and 20 mg/day) was given for five days starting from the 16th day. Changes in both enzyme activities in the serum, placental cytosol and microsome caused by the steroids were analyzed. Aminopeptidase A activities in all three compartments and leucine aminopeptidase activities in the serum and cytosol were stimulated by estradiol (1.0 mg/day) plus progesterone (10 mg/day). Since we previously found that the placental aminopeptidases degrade peptide hormones, our present study suggests that steroids effect the metabolism of peptide hormones of fetal and/or maternal origin.
Kurauchi
O
O
Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Japan.
Mizutani
S
S
Nomura
S
S
Hotta
R
R
Ito
Y
Y
Asada
Y
Y
Furuhashi
M
M
Kasugai
M
M
Tomoda
Y
Y
D000626:Aminopeptidases / Q000097:blood / Q000378:metabolism*; D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D043384:Glutamyl Aminopeptidase; D007931:Leucyl Aminopeptidase / Q000097:blood / Q000378:metabolism*; D010920:Placenta / Q000187:drug effects / Q000201:enzymology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000097:blood*; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats
D011374:Progesterone; D004958:Estradiol; D000626:Aminopeptidases; D007931:Leucyl Aminopeptidase; D043384:Glutamyl Aminopeptidase