title: Differential hypomethylation of the c-myc protooncogene in bladder cancers at different stages and grades.
abstract: The 5-methylcytosine content of c-myc proto-oncogene DNA obtained from samples of normal bladders and of bladders with transitional cell carcinoma of different stage and grade was analyzed. Five CCGG sites (from the third coding region to the 3' flanking region of the gene) which showed different methylation in normal and neoplastic samples were identified. The overall c-myc methylation levels were significantly reduced in carcinomas. Furthermore, a significant correlation between disease invasiveness and methylation level was found. Nevertheless, in each group of patients with the same histological grade, heterogeneity has been observed. This might be suggestive of different prognosis of the disease.
authors.affiliation: Centro di Studi Biochimici delle Patologie del genoma umano, Università, Ferrara, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Del Senno
authors.forename: I
authors.initials: I
authors.lastname: Maestri
authors.forename: R
authors.initials: R
authors.lastname: Piva
authors.forename: S
authors.initials: S
authors.lastname: Hanau
authors.forename: A
authors.initials: A
authors.lastname: Reggiani
authors.forename: A
authors.initials: A
authors.lastname: Romano
authors.forename: G
authors.initials: G
authors.lastname: Russo
mesh_terms: D044503:5-Methylcytosine; D001345:Autoradiography; D015139:Blotting, Southern; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D003596:Cytosine / Q000031:analogs & derivatives / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009857:Oncogenes; D011379:Prognosis; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D044503:5-Methylcytosine; D003596:Cytosine