title: Effects of metal ions on cyprinid fish immune response: in vitro effects of Zn2+ and Mn2+ on the mitogenic response of carp pronephros lymphocytes.
abstract: Lymphocytes from the pronephros of carp (Cyprinus carpio L) have been subjected to transformation by mitogens, concanavalin A (Con A), phytohemagglutinin (PHA), and lipopolysaccharides (LPS), with Zn or Mn at varying concentrations. Addition of Zn2+ (10(-7) to 10(-3) M) to mitogen-stimulated T and B cells enhanced [3H]thymidine incorporation. Addition of 10(-5) M Zn2+ inhibited the response to Con A, PHA, and LPS. At this concentration, Zn was toxic. Addition of Mn2+ (10(-7) to 10(-3) M) to mitogen-stimulated lymphocytes enhanced [3H]thymidine incorporation. This effect was observed with Con A- and PHA-stimulated lymphocytes, but not with LPS-stimulated lymphocytes. In contrast, addition of 10(-1) M Mn2+ to lymphocyte cultures exerted an inhibitor on the response to Con A or to PHA, while the response to LPS was unaffected. Addition of 10(-1) M Mn2+ to Con A- or PHA-stimulated cultures at different times after initiation of stimulation indicated that Mn2+ was inhibitory only when it was added before the first 16 hr of cultures. The inhibition induced by 10(-1) M Mn2+ could be reversed by adding 2 mM CaCl2 to the culture.
authors.affiliation: Laboratoire d'Immunophysiologie générale et comparée, U.E.R. des sciences, Limoges, France.
authors.forename: Z
authors.initials: Z
authors.lastname: Ghanmi
authors.forename: M
authors.initials: M
authors.lastname: Rouabhia
authors.forename: O
authors.initials: O
authors.lastname: Othmane
authors.forename: P A
authors.initials: PA
authors.lastname: Deschaux
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D003530:Cyprinidae / Q000276:immunology*; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008274:Magnesium / Q000633:toxicity*; D008938:Mitosis / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D014873:Water Pollutants / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000633:toxicity*
chemical_list: D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D003208:Concanavalin A; D008274:Magnesium; D015032:Zinc; D002118:Calcium