title: Tropomyosin isoforms in developing chicken gizzard smooth muscle.
abstract: In the embryonic smooth muscle of chicken gizzards we found 4 high-Mr-type and 5 low-Mr-type tropomyosin isoforms in addition to alpha- and beta-isoforms reported already. The criteria by which they were identified as tropomyosin isoforms were as follows: 1) anomalous reduction of electrophoretic mobility in the presence of urea, 2) cross reactivity with antisera against tropomyosins, 3) inclusion in a tropomyosin preparation obtained by the usual method for tropomyosin purification, and 4) binding ability to skeletal muscle actin. At the early stages of development, the amounts of these isoforms were larger than those of alpha- and beta-isoforms, but they gradually decreased at later stages and finally disappeared completely after hatching. Our previous study of gizzard smooth muscle showed that the amount ratio of accumulated tropomyosin to gamma-actin was reasonably constant in the development after hatching, while, at the earlier embryonic stages (7-14 d of incubation), it was lower than expected. The isoforms found in this study were present in amounts large enough to bring the ratio at the earlier stages up to the constant amount ratio observed after hatching. Therefore, the coordinate accumulation of actin and tropomyosin was suggested to occur even at the embryonic stages.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Ibaraki.
authors.forename: M
authors.initials: M
authors.lastname: Hosoya
authors.forename: J
authors.initials: J
authors.lastname: Miyazaki
authors.forename: T
authors.initials: T
authors.lastname: Hirabayashi
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002642:Chick Embryo; D015180:Electrophoresis, Gel, Two-Dimensional; D005895:Gizzard, Avian / Q000196:embryology / Q000378:metabolism*; D015151:Immunoblotting; D007536:Isomerism; D009130:Muscle, Smooth / Q000196:embryology / Q000378:metabolism*; D014335:Tropomyosin / Q000032:analysis* / Q000276:immunology
chemical_list: D000199:Actins; D000906:Antibodies; D014335:Tropomyosin