title: Autocrine regulation of bovine retinal capillary endothelial cell (BREC) proliferation by BREC-derived basic fibroblast growth factor.
abstract: Fibroblast growth factors (FGFs) are mitogenic for bovine retinal capillary endothelial cells (BREC) seeded at a low density. Seeding BREC cells at a high density greatly reduces their requirement for basic FGF (bFGF) in order to proliferate actively. We show here that monolayers of BREC cells synthesize and release into the culture medium a growth factor, which on the basis of biological activity, heparin affinity, immuno-cross reactivity with anti-bFGF antibodies and mRNA analysis, has been identified as basic fibroblast growth factor. These data indicate that BREC cells are able to synthesize and release bFGF, which can act as a promoting-growth factor for these cells by a para- and/or autocrine mechanism. We suggest thus, that this para- and/or autocrine mechanism involving bFGF may play a key role in preretinal neovascularization, particularly in diabetic patients presenting a proliferative retinopathy.
authors.affiliation: INSERM U 168, Laboratoire d'Endocrinologie Expérimentale, CHU Rangueil, Université Paul Sabatier, Toulouse, France.
authors.forename: M
authors.initials: M
authors.lastname: Bensaid
authors.forename: F
authors.initials: F
authors.lastname: Malecaze
authors.forename: H
authors.initials: H
authors.lastname: Prats
authors.forename: F
authors.initials: F
authors.lastname: Bayard
authors.forename: J P
authors.initials: JP
authors.lastname: Tauber
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000494:pharmacology / Q000502:physiology*; D012171:Retinal Vessels / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D005346:Fibroblast Growth Factors