Blocking of lectin-like adhesion molecules on pulmonary cells inhibits lung sarcoma L-1 colonization in BALB/c-mice.
Adhesion and inhibition experiments with pulmonary cells of BALB/c-mouse origin and syngeneic sarcoma L-1 cells indicated that L-fucose specific lectin-like adhesion molecules, presumably situated on pulmonary cell surfaces are (at least partly) responsible for the specificity of this cell-cell interaction. Addition of specific sugars and glycoconjugates (L-fucose and fucoidan, respectively) to the incubation medium evidently inhibited the adhesion process as quantified using radiolabelled tumor cells. Unspecific carbohydrates (e.g. D-galactose) did not affect the cellular interaction. In vivo, repeated administration of fucoidan (but not of unspecific glycoconjugates) significantly inhibited the settling of metastatic sarcoma L-1 cells in the lungs of BALB/c-mice. Therefore, when lectin-like adhesion molecules on pulmonary cells were blocked with competitive glycoconjugates, tumor cell colonization of the lung could be significantly inhibited.
Roszkowski
W
W
National Institute of Lung Diseases and Tuberculosis, Warsaw, Poland.
Beuth
J
J
Ko
H L
HL
Uhlenbruck
G
G
Pulverer
G
G
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D006001:Glycoconjugates / Q000494:pharmacology; D037102:Lectins / Q000502:physiology*; D008168:Lung / Q000166:cytology*; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012513:Sarcoma, Experimental / Q000473:pathology*; D014407:Tumor Cells, Cultured
D006001:Glycoconjugates; D037102:Lectins