Measurement of vitamin K in human liver by gradient elution high-performance liquid chromatography using platinum-black catalyst reduction and fluorimetric detection.
A sensitive and precise method for measuring endogenous phylloquinone (K1) and menaquinone (MK-n) in human liver was developed, based on gradient elution high-performance liquid chromatography using platinum-black catalyst reduction and fluorimetric detection. Subnanogram levels of vitamin K compounds in 1 g of liver specimen were detectable. We measured vitamin K concentrations in 38 human resected livers. K1 and MK-4 to MK-13 were detected. The concentrations of MK-10 to MK-12 in livers with chronic hepatitis (n = 10) and cirrhosis (n = 22) were significantly lower than in normal livers (n = 6). It is suggested that the decreased concentrations indicate functional damage of the hepatocytes.
Usui
Y
Y
Second Department of Surgery, Kyoto University Faculty of Medicine, Japan.
Nishimura
N
N
Kobayashi
N
N
Okanoue
T
T
Kimoto
M
M
Ozawa
K
K
D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008099:Liver / Q000032:analysis*; D008930:Mitochondria, Liver / Q000032:analysis; D010084:Oxidation-Reduction; D010984:Platinum; D013050:Spectrometry, Fluorescence; D014812:Vitamin K / Q000032:analysis*; D010837:Vitamin K 1 / Q000032:analysis
D014812:Vitamin K; D010984:Platinum; D010837:Vitamin K 1