[Substrate specificity of T4 RNA-ligase. The effect of the nucleotide composition of substrates and the size of phosphate donor on the effectiveness of intermolecular ligation].
Nucleoside 2' (3'),5'-diphosphates, dinucleotides pApA, pApC, pApU, pGpC, pCpC, pUpU (phosphate donors), and trinucleoside diphosphates, such as NpCpC, NpCpU, NpUpC, NpUpU and GpApN (N = U, C, A or G; phosphate acceptors) were used to study the substrate specificity of T4 RNA ligase. Relative efficiency of the mono- and dinucleotide donors depends on the 5'-terminal nucleoside moiety of the dinucleotide: upon ligation with the minimal phosphate acceptor GpUpC, dinucleotides pApA, pApC, and pApU are more effective than nucleotide diphosphate pAp; pGpC is more effective than pGp; efficiencies of pCpC and pCp are almost identical, and efficiency of pUpU is slightly lower than that of pUp. In relative efficiency, dinucleotide donors, varying only in 5'-terminal unit, do not correspond to mononucleotides: pApC greater than pCpC greater than pGpC and pCp greater than pUp approximately pAp much greater than pGp. The effects observed for homooligomeric substrates cannot be extra-polated on heterooligomers.
Zhenodarova
S M
SM
Kliagina
V P
VP
Maistrenko
F G
FG
Pustoshilova
N M
NM
Smolianinova
O A
OA
D002848:Chromatography, DEAE-Cellulose; D008024:Ligands; D009711:Nucleotides; D010710:Phosphates; D011118:Polynucleotide Ligases / Q000378:metabolism*; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D013379:Substrate Specificity; D013604:T-Phages / Q000201:enzymology*
D008024:Ligands; D009711:Nucleotides; D010710:Phosphates; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP)
Substratnaia spetsifichnost RNK-ligazy T4. Vliianie nukleotidnogo sostava substratov i razmera donora fosfata na éffektivnost' mezhmolekuliarnogo ligirovaniia.