[Effect of vitamin E-containing liposome on experimental sugar cataract].
The effect of vitamin E-containing liposome on experimental sugar cataract formation in vitro was investigated. The lenses of male Wistar rats aged 6 weeks were prepared by incubating with 55. 6 mM glucose with vitamin E-containing liposome (dipalmitoyl phosphatidylcholine: DPPC). We examined the formation of lens opacity and assayed vitamin E and its related compounds. Incubation with vitamin E-containing liposome prevented sugar cataract formation. In Addition, vitamin E concentration in lens was significantly elevated by incubation with vitamin E-containing liposome. In lenses of the high level glucose group incubated without vitamin E-containing liposome, lipid peroxide (LPO) content was increased, but in lenses of the high-level glucose group incubated with vitamin E-containing liposome the increase was significantly inhibited at each incubation time. Vitamin E had no effect either on the decrease of reduced glutathione (GSH) or the increase of sorbitol content in lens incubated with high level glucose medium. In conclusion vitamin E-containing liposome was transported from medium to lens well and was significantly effective in preventing experimental sugar cataract formation in vitro. The protective effects of vitamin E are probably caused not only by its antioxidative action.
Hattori
H
H
Majima
Y
Y
Nagamura
Y
Y
Ishiguro
I
I
D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000517:prevention & control*; D004337:Drug Carriers; D005947:Glucose; D066298:In Vitro Techniques; D008081:Liposomes; D008297:Male; D051381:Rats; D014810:Vitamin E / Q000008:administration & dosage* / Q000494:pharmacology
D004337:Drug Carriers; D008081:Liposomes; D014810:Vitamin E; D005947:Glucose