[Interaction of pyruvate dehydrogenase complex from the heart muscle with thiamine diphosphate and its derivatives].
Inhibitory effects of 23 thiamin derivatives on the bovine heart pyruvate dehydrogenase complex (PDC) were studied. Oxythiamin diphosphate and tetrahydroxythiamin diphosphate exhibited the most pronounced effect on the PDC activity, affecting the complex by a competitive type of inhibition for thiamin diphosphate (TDP). The apparent affinity of TDP and the anticoenzyme derivatives for apo PDC depended on presence of phosphate and divalent metal ions. Phosphate considerably increased the Km values for TDP (up to 0.17 microM) and the Ki values for oxythiamin diphosphate (0.40 microM) as well as for tetrahydroxythiamin diphosphate (0.23 microM). In presence of Mn2+, Km value for TDP was 3.5-fold lower as compared with Mg2+ containing medium.
Strumilo
S A
SA
Kiselevskiĭ
Iu V
IuV
Taranda
N I
NI
Zabrodskaia
S V
SV
Oparin
D A
DA
D000818:Animals; D002384:Catalysis; D002417:Cattle; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology*; D010084:Oxidation-Reduction; D011768:Pyruvate Dehydrogenase Complex / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013835:Thiamine Pyrophosphate / Q000031:analogs & derivatives / Q000378:metabolism*
D011768:Pyruvate Dehydrogenase Complex; D008345:Manganese; D008274:Magnesium; D013835:Thiamine Pyrophosphate
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