[Study of microcirculatory changes in experimental dietary fatty liver].
Regional hepatic blood flow in SD rats with fatty liver was measured by electrolytic regional blood flow meter (Biomedical Sci. Co. Ltd. model RBF-2) and compared with areas of sinusoids histologically calculated by CONTRON MOP-AM 03.


METHODS
SD male rats, weighing around 240 g, fed on the choline deficient diet, were anaesthetized with ethyl ether and rough regional hepatic blood flow were measured after 3, 5 and 7 weeks. Furthermore, after 15 min. from death, they were measured (death regional flow) in order to determine the simple diffusion of hydrogen gas in liver tissue. Real regional blood flow was got by subtraction of death regional flow from rough regional blood flow. The degree of fatty liver was histologically divided into 3 groups, as it were, slight, moderate and severe. The lipid was extracted from fatty liver in 3 groups using the combined liquids of methanol and chloroform, and the weight of the lipid was measured to compare with fatty changes. The area of sinusoids in fatty liver was analyzed from microscopic pictures using CONTRON MOP-AM 03.


RESULTS
There was no difference of rough regional hepatic blood flow among 3 groups as shown Figure.: (table; see text) But the real regional blood flow was decreased in the moderate or severe groups. And the lipid weights of the liver tissues were increased according to severe fatty changes. The values of real regional blood flow were well-correlated with decrease of the areas of sinusoids histologically analyzed. These facts suggested that fat vacuoles narrowed the lumen of sinusoid and decreased the hepatic blood flow.
Ohhara
K
K
3rd Department of Internal Medicine, Asahikawa Medical College, Japan.
D000818:Animals; D002796:Choline Deficiency; D004032:Diet; D005234:Fatty Liver / Q000503:physiopathology*; D008099:Liver / Q000098:blood supply*; D008297:Male; D008833:Microcirculation; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow