High voltage electric pulses efficiently induce fusion of cells in monolayer culture.
High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency of 3 X 10(-4) - 2 X 10(-5) after culturing fused cells in a selection medium that permits the growth of only hybrid cells. This method of electrofusion was applied for complementation analysis of DNA repair-deficient xeroderma pigmentosum cells. Efficient cell fusion was also observed with these human diploid fibroblasts and the resulting heterodikaryons showed a recovery of ultraviolet light-induced unscheduled DNA synthesis to the same extent as in normal cells.
Ishizaki
K
K
Radiation Biology Center, Kyoto University, Japan.
Chang
H R
HR
Eguchi
T
T
Ikenaga
M
M
D000818:Animals; D002459:Cell Fusion; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D004560:Electricity; D005816:Genetic Complementation Test; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology* / Q000378:metabolism; D014983:Xeroderma Pigmentosum / Q000235:genetics / Q000378:metabolism / Q000473:pathology
D004247:DNA