Complementation of a Dictyostelium discoideum thymidylate synthase mutation with the mouse gene provides a new selectable marker for transformation.
A cDNA encoding mouse thymidylate synthase has been inserted 3' to the Dictyostelium discoideum actin 15 promoter in an E. coli-D.discoideum shuttle vector. When this construct was introduced into a D.discoideum thymidylate synthase mutant strain HPS400, stable transformants were obtained at high frequency. These transformants grew in standard axenic medium without requiring exogenous thymidine. This construct provides a second selectable marker for use in transformation of D.discoideum.
Chang
A C
AC
School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Williams
K L
KL
Williams
J G
JG
Ceccarelli
A
A
D000818:Animals; D004023:Dictyostelium / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D051379:Mice; D009154:Mutation; D015183:Restriction Mapping; D013940:Thymidylate Synthase / Q000235:genetics*; D014170:Transformation, Genetic
D013940:Thymidylate Synthase