Investigations on the interferon-induced 2'-5' oligoadenylate system using analytical capillary isotachophoresis.
The activity of the interferon inducible enzyme 2'-5' oligoadenylate synthetase (2-5A synthetase, E.C. 2.7.7) which converts ATP into a series of 2'-5' oligoadenylates was measured using analytical capillary isotachophoresis. The turnover rate of ATP during the reaction was monitored by determination of its concentration at the beginning and the end of the 2-5A synthetase reaction. The enzyme was analysed in extracts of peripheral blood mononuclear cells either pretreated or not with interferon.
Bruchelt
G
G
Children's Hospital, Department of Hematology, University of Tuebingen, F.R.G.
Buedenbender
M
M
Treumer
J
J
Niethammer
D
D
Schmidt
K
K
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis*; D000227:Adenine Nucleotides / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D004586:Electrophoresis; D007370:Interferon Type I / Q000494:pharmacology*; D008214:Lymphocytes / Q000032:analysis; D009843:Oligoribonucleotides / Q000378:metabolism
D000227:Adenine Nucleotides; D007370:Interferon Type I; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D015088:2',5'-Oligoadenylate Synthetase