Histidine release from brain slices and synaptosomes.
The release of radioactive histidine was studied in a superfusion system by using preloaded rat cerebral cortical slices and guinea pig synaptosomes. ATP/ADP ratios in both preparations were used to assess the energy levels and thus the oxygenation of brain slices and synaptosomes. ATP/ADP ratios similar to the intact brain and nerve terminals were found in slices and synaptosomes even after hours of superfusion with oxygenated buffer. Depolarization by 30 or 50 mM K+ induced release of histidine from slices and synaptosomes. The results confirm that under physiological energetic conditions, histidine is released both from slices and synaptosomes after depolarization with potassium.
Tuomisto
L
L
Department of Pharmacology and Toxicology, University of Kuopio, Finland.
Antikainen
T
T
Raatikainen
O
O
Kauppinen
R
R
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D006168:Guinea Pigs; D006639:Histidine / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
D006639:Histidine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D011188:Potassium