title: Expression and characterization of the tau subunit of phosphorylase kinase.
abstract: A cDNA encoding the entire tau subunit of rabbit skeletal muscle phosphorylase kinase was reconstructed and inserted into a plasmid containing the Escherichia coli ptac promoter and a constructed plasmid containing the ptac promoter and bacterial chloramphenicol acetyl transferase (CAT) gene, respectively. A significant phosphorylase kinase activity was found, in the first case. In the second case, a fused protein containing 73 amino acids from the CAT protein was obtained. After renaturation, the CAT-tau subunit protein shows enzymatic activity similar to the HPLC-purified and renatured tau subunit.
authors.affiliation: Dept. of Biochem. and Biophys., Iowa State Univ., Ames 50011.
authors.forename: L R
authors.initials: LR
authors.lastname: Chen
authors.forename: C J
authors.initials: CJ
authors.lastname: Yuan
authors.forename: G
authors.initials: G
authors.lastname: Somasekhar
authors.forename: P
authors.initials: P
authors.lastname: Wejksnora
authors.forename: J E
authors.initials: JE
authors.lastname: Peterson
authors.forename: A M
authors.initials: AM
authors.lastname: Myers
authors.forename: L
authors.initials: L
authors.lastname: Graves
authors.forename: P T
authors.initials: PT
authors.lastname: Cohen
authors.forename: E F
authors.initials: EF
authors.lastname: da Cruz e Silva
authors.forename: D J
authors.initials: DJ
authors.lastname: Graves
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D007700:Kinetics; D046911:Macromolecular Substances; D010764:Phosphorylase Kinase / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D004247:DNA; D010764:Phosphorylase Kinase