[Application of immunoelectron microscopy to the diagnosis of kidney diseases].
The paper considers various modes of fixation and treatment of renal tissue biopsies for immunoelectron microscopic examinations. A simple and effective procedure was proposed to detect immunoglobulins and complement components at the ultrastructural level. The biopsy specimens were fixed with a mixture of 2% paraformaldehyde and 0.05% glutaraldehyde with 0.05% saponin, then they were impregnated with increasing concentrations of glycerin and frozen. Immunoglobulins and complement components were identified by indirect immunoperoxidase method using cryostat sections.
Ivanov
A A
AA
Pal'tsev
M A
MA
Razgulina
L E
LE
Poklonova
N V
NV
D000818:Animals; D001706:Biopsy; D003165:Complement System Proteins / Q000032:analysis; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007674:Kidney Diseases / Q000175:diagnosis*; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
D007136:Immunoglobulins; D003165:Complement System Proteins
Ispol'zovanie immunoélektronnoĭ mikroskopii pri diagnostike zabolevaniĭ pochek.