The mouse homologue of hst/k-FGF: sequence, genome organization and location relative to int-2.
We have located and sequenced the murine homologue of the hst/k-FGF oncogene in genomic DNA clones that extend 3' from int-2 on mouse chromosome 7. The two genes are in the same transcriptional orientation, less than 20 kilobase pairs (kb) apart, and presumably evolved by tandem duplication of a common ancestral gene. RNase mapping and primer extension analyses indicated that the major 3.2 kb hst transcript expressed in undifferentiated embryonal carcinoma (EC) cell lines initiates at a unique cap site downstream from an obvious TATA-box. The 3' end of the transcript as identified in multiple cDNA clones occurs at an appropriate distance from a variant polyadenylation signal, ATTAAA. Translation of the major open reading frame would yield a 202 amino acid protein that is 82% homologous to human HST but lacking 4 residues at the presumed signal peptide cleavage site.
Brookes
S
S
Imperial Cancer Research Fund Laboratories, St Bartholomew's Hospital, London, UK.
Smith
R
R
Thurlow
J
J
Dickson
C
C
Peters
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000302:isolation & purification; D005796:Genes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D005346:Fibroblast Growth Factors; D004247:DNA