Identification by C-banding of two human prostate tumour cell lines, 1013L and DU 145.
Two human prostate carcinoma cell lines are easily distinguished by C-banding. DU 145 has one metacentric chromosome with a large centromeric band, 1 metacentric and 2 acrocentric chromosomes with interstitial C-bands. The Y chromosome was present in 48% and 42% of the cells at passages 83 and 106, respectively, whereas in 1013L only one metacentric chromosome contained distinct extra C bands--one at the centromere and one interstitially. The Y chromosome was present in 72% of the cells at passage 15 and in 20% at passage 22, but by passage 44 it had disappeared. Karyotype analysis using G-banding revealed that DU 145 had retained several of its original markers, and demonstrated multiple marker chromosomes in 1013L.
Hartley-Asp
B
B
Pharmacia LEO Therapeutics AB, Department of Cancerpharmacology, Helsingborg, Sweden.
Billström
A
A
Kruse
E
E
D002869:Chromosome Aberrations; D002871:Chromosome Banding; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured
D005819:Genetic Markers