Analytical determination of methylated histidine in proteins: actin methylation.
The methylation of histidine in actin from various muscle and nonmuscle sources has been studied by formation of phenylthiocarbamyl derivatives and subsequent reverse-phase high-pressure liquid chromatographic separation and analysis of actin hydrolyzates. All the actin species examined were found to contain 3-methylhistidine. This method has also been used in assays for the enzyme(s) responsible for methylation of rabbit skeletal muscle actin and to investigate the formation of other methylated residues in vitro. 3-Methyl-histidine is the major methylation product in this in vitro reaction.
Raghavan
M
M
Department of Chemistry, Princeton University, New Jersey 08544.
Smith
C K
CK
Schutt
C E
CE
D000199:Actins / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005895:Gizzard, Avian / Q000032:analysis; D006639:Histidine / Q000031:analogs & derivatives*; D008762:Methylhistidines / Q000032:analysis*; D009132:Muscles / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D011817:Rabbits; D013154:Spleen / Q000032:analysis; D013552:Swine
D000199:Actins; D000596:Amino Acids; D008762:Methylhistidines; D006639:Histidine