Nucleotide concentrations in hepatocytes during anoxia and reoxygenation in presence of allopurinol and oxypurinol.
In hepatocytes of starved rats nucleotide, nucleoside and nucleobase concentrations were measured during and following anoxia. A complete restoration of ATP is observed during reoxygenation after anoxic periods less than 60 minutes. GTP cannot be completely restored after 30 minutes of anoxia. Allopurinol and oxypurinol do not accelerate the ATP and GTP restoration. IMP, adenosine and AMP concentrations are increased in presence of allopurinol or oxypurinol during anoxia and reoxygenation.
Schneider
W
W
Institut für Biochemie, Bereich Medizin Charité, Humboldt-Universität, Berlin, DDR.
Grune
T
T
Siems
W
W
Werner
A
A
Gerber
G
G
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000493:Allopurinol / Q000494:pharmacology*; D000693:Anaerobiosis; D000818:Animals; D002478:Cells, Cultured; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010117:Oxypurinol / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation
D007042:Hypoxanthines; D011743:Pyrimidines; D019271:Hypoxanthine; D000493:Allopurinol; D000255:Adenosine Triphosphate; D010117:Oxypurinol