title: Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever.
abstract: A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.
authors.affiliation: Department of Bacterial Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
authors.forename: F A
authors.initials: FA
authors.lastname: Rubin
authors.forename: P D
authors.initials: PD
authors.lastname: McWhirter
authors.forename: N H
authors.initials: NH
authors.lastname: Punjabi
authors.forename: E
authors.initials: E
authors.lastname: Lane
authors.forename: P
authors.initials: P
authors.lastname: Sudarmono
authors.forename: S P
authors.initials: SP
authors.lastname: Pulungsih
authors.forename: M
authors.initials: M
authors.lastname: Lesmana
authors.forename: S
authors.initials: S
authors.lastname: Kumala
authors.forename: D J
authors.initials: DJ
authors.lastname: Kopecko
authors.forename: S L
authors.initials: SL
authors.lastname: Hoffman
mesh_terms: D001345:Autoradiography; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*; D018805:Sepsis / Q000175:diagnosis / Q000382:microbiology*; D014435:Typhoid Fever / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial