[Comparison of antigenic properties of the X-potato virus and its coat proteins. Effect of proteolytic cleavage on antigenic characteristics].
Antigenic properties of intact potato virus X (PVX) particles and of PVX coat protein (CP) preparations were compared using different modifications of ELISA test. In the competitive ELISA test (reaction in solution) antibodies to intact virus react much stronger with PVX than with CP while antibodies to CP react much stronger with CP than with PVX. In the direct ELISA test (reaction on the solid support) the difference in reactions of antiCP antibodies with PVX and CP is eliminated while the one in reactions of antiPVX antibodies with these antigens remains. No difference was registered in reactivity of PVX absorbed directly on polystyrene or on immunoglobulin-coated wells (sandwich ELISA) to antiCP antibodies.
Bobkova
A F
AF
Gol'dshteĭn
Kaftanova
A S
AS
Dobrov
E N
EN
Atabekov
I G
IG
D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D002213:Capsid / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006868:Hydrolysis; D010942:Plant Viruses / Q000276:immunology*; D011198:Solanum tuberosum / Q000382:microbiology
D000914:Antibodies, Viral; D000956:Antigens, Viral
Sravnenie antigennykh svoĭstv x-virusa kartofelia i ego belka obolochki. Vliianie proteoliticcheskogo rasshchepleniia na antigennye kharakteristiki.