Profiling of Alternaria mycotoxins in foodstuffs by high-performance liquid chromatography with diode-array ultraviolet detection.
The potential of high-performance liquid chromatography (HPLC) with diode-array detection for the profiling of mycotoxins in food samples has been demonstrated. A gradient elution reversed-phase chromatographic method coupled with a suitable extraction procedure was devised for the separation and detection of major Alternaria mycotoxins in foodstuffs. Altenuene, alternariol, alternariol methyl ether (dibenzo-alpha-pyrone derivatives), altertoxin-I and altertoxin-II (perylene derivatives) were profiled in extracts of artificially infected maize, rice and tomato samples and naturally contaminated sunflower seeds. First evidence of the occurrence of a new dibenzo-alpha-pyrone derivative in Alternaria cultures is also reported.
Palmisano
F
F
Laboratorio di Chimica Analitica, Dipartimento di Chimica dell'Università, Bari, Italy.
Zambonin
P G
PG
Visconti
A
A
Bottalico
A
A
D000528:Alternaria / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D003904:Mitosporic Fungi / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D009183:Mycotoxins