Do cadmium and dexamethasone induce different types of metallothioneins?
1. Dexamethasone induced thionein in rat liver cross reacts with antibody made towards Cd-thionein from rat liver. 2. Dex-thionein binds Cd. 3. Compared to Cd-thionein, dex-thionein behaves differently on SDS polyacrylamide gels. 4. On molecular filtration on Sephadex G-75 columns, dex-thionein elutes slightly more towards the low molecular weight region than Cd-thionein. 5. Dex-thionein elutes in one peak with two shoulders after DEAE-Sephadex A-50 ion exchange chromatography, while Cd-thionein elutes in two peaks.
Andersen
R A
RA
Department of Toxicology, National Institute of Public Health, Oslo, Norway.
Daae
H L
HL
Mikalsen
A
A
Alexander
J
J
D000818:Animals; D015153:Blotting, Western; D002104:Cadmium / Q000494:pharmacology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003907:Dexamethasone / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D008668:Metallothionein / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains
C004203:cadmium-binding protein; D002104:Cadmium; D003907:Dexamethasone; D008668:Metallothionein