Analysis of human metaphase chromosomes using antibodies to double-stranded and single-stranded DNA: staining patterns are related to DNA conformation.
Fresh and 6-day-old fixed chromosome spreads, both untreated and treated with various banding techniques and nucleases, were stained using monoclonal antibodies to double-stranded and single-stranded DNA. DNA in fixed chromosome preparations became progressively denatured with ageing. The staining pattern of untreated chromosomes with anti-double-stranded DNA antibodies (which resembles G-banding) was determined by the conformation of the chromosomal DNA.
Mezzanotte
R
R
Istituto di Biologia Generale, Università di Cagliari, Italy.
Peretti
D
D
Ennas
M G
MG
Vanni
R
R
Sumner
A T
AT
D000911:Antibodies, Monoclonal / Q000032:analysis*; D002877:Chromosomes, Human / Q000648:ultrastructure*; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D009690:Nucleic Acid Conformation
D000911:Antibodies, Monoclonal; D004277:DNA, Single-Stranded; D004247:DNA