The effect of chronic parenteral administration of cadmium on isoenzyme levels of alkaline phosphate in intestinal mucosa.
Chronic administration of cadmium chloride to rats (13.3 mumol/kg body wt per dose subcutaneously) produced a decrease in the activity of alkaline phosphatase in the intestinal mucosa to less than half that in control rats by the time cumulative doses of between 30 and 48 mumol had been administered. The reduced level of activity remained approximately steady following further dosing. Three isoenzymes of intestinal alkaline phosphatase were separated electrophoretically. Chronic cadmium treatment markedly decreased the proportion of the 2 isoenzymes with lower electrophoretic mobility. Some analogies are drawn between the effect of cadmium administration, and magnesium deficiency on changes in intestinal alkaline phosphatase.
O'Brien
I G
IG
Department of Biochemistry, University of Surrey, Guildford, U.K.
King
L J
LJ
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D004347:Drug Interactions; D007263:Infusions, Parenteral; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000201:enzymology; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D002104:Cadmium; D000469:Alkaline Phosphatase