Two-step high-performance liquid chromatography method for the determination of myo-inositol and sorbitol.
A simple two-step HPLC method for the separation and quantitation of myo-inositol and sorbitol in extracts of glomeruli from rat kidneys is described. The limit of detection is 2 ng. The procedure involves fractionation of the sugar alcohols on a Waters Sugar Pak column, preparation of the p-nitrobenzoate derivatives, and further purification with quantitation by absorbance at 254 nm using a Waters mu Porasil column. The applicability of the procedure to determination of sorbitol and myo-inositol in biological samples was demonstrated by the finding of marked alterations in sorbitol and myo-inositol content of glomeruli isolated from diabetic compared to that from normal rat kidneys.
Lauro
P N
PN
Department of Medicine, Veterans Administration Medical Center, Pittsburgh, Pennsylvania 15240.
Craven
P A
PA
DeRubertis
F R
FR
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005260:Female; D005632:Fructose / Q000032:analysis; D007294:Inositol / Q000032:analysis*; D007678:Kidney Glomerulus / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013012:Sorbitol / Q000032:analysis*
D005632:Fructose; D007294:Inositol; D013012:Sorbitol