title: Reaction of Se with SH groups in spectrin is involved in the stabilization of erythrocyte membrane skeleton.
abstract: Na2SeO3 supplementation in the dialysis medium could obviously prevent the dissociation of spectrin from the erythrocyte membranes. Such Se effect could be eliminated by pretreatment of erythrocyte membranes with a SH-blocking reagent, iodoacetamide(IAA) or addition of a SH-compound, dithio-threitol. The fluorescence intensity of erythrocyte membranes labelled with the fluorescent probe N-(3-pyrenyl)-maleimide decreased with increasing Na2SeO3 concentration used for pretreatment of ghosts. 31P-NMR spectra of erythrocyte membrane dialyzed in the presence or absence of Na2SeO3 concentration showed a difference in chemical shift anisotropy (delta sigma) between these two samples. These data suggest that the stabilization effect is based on changes in lipid-protein interaction and conformation of membrane skeletal components induced by reaction of their SH groups with Na2SeO3.
authors.affiliation: Institute of Biophysics, Academia Sinica, Beijing, China.
authors.forename: F Y
authors.initials: FY
authors.lastname: Yang
authors.forename: M Z
authors.initials: MZ
authors.lastname: Yang
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D015314:Dialysis Solutions / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006801:Humans; D007460:Iodoacetamide / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D012643:Selenium / Q000378:metabolism*; D013049:Spectrin / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D015314:Dialysis Solutions; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D013049:Spectrin; D012643:Selenium; D007460:Iodoacetamide