Metabolic interfaces between growth and reproduction. II. Characterization of changes in messenger ribonucleic acid concentrations of gonadotropin subunits, growth hormone, and prolactin in nutritionally growth-limited lambs and the differential effects of increased nutrition.
Previous studies show that limited nutrition in the ovariectomized lamb results in an impairment of LH and FSH secretion, a phenomenon that is rapidly reversible by increasing the level of nutrition and independent of ovarian steroid feedback. The present study characterizes the biosynthesis of pituitary hormones in the nutritionally growth-limited female lamb by measuring steady state mRNA concentrations. These changes were examined relatively to pituitary and serum hormone concentrations to establish the relationship(s) of synthesis and secretion in response to nutritional manipulation. Ad libitum feeding of nutritionally growth-restricted ovariectomized lambs for 14 days resulted in an increase in the frequency of episodic LH release, thereby increasing mean serum LH concentrations (P less than 0.001). Similarly, mean circulating concentrations of FSH were increased (P less than 0.001). By contrast, serum GH concentrations were lowered significantly as a result of ad libitum feeding (P less than 0.05). Serum PRL concentrations remained unchanged. Although pituitary LH and PRL concentrations were also unchanged in response to increased nutrition feeding, FSH and GH concentrations increased (P less than 0.05). Short-term ad libitum feeding of the chronically food-restricted lamb resulted in significant changes in mRNA concentrations for all hormones except PRL. The concentrations of gonadotropin subunit mRNAs (i.e. alpha, LH beta, and FSH beta) were all significantly higher in response to increased nutrition (P less than 0.001). GH mRNA was also affected; however, feeding decreased concentrations (P less than 0.001). The results demonstrate that an increase in the level of nutrition in the restricted diet lamb produces profound changes in the synthesis, storage, and secretion of LH, FSH, and GH. These changes appear to be coordinated, although the response differs depending upon the hormone.
Landefeld
T D
TD
Department of Pharmacology, University of Michigan, Ann Arbor 48109.
Ebling
F J
FJ
Suttie
J M
JM
Vannerson
L A
LA
Padmanabhan
V
V
Beitins
I Z
IZ
Foster
D L
DL
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D005508:Food Deprivation / Q000502:physiology; D006062:Gonadotropins / Q000097:blood / Q000235:genetics*; D006128:Growth; D006130:Growth Disorders / Q000235:genetics*; D013006:Growth Hormone / Q000097:blood / Q000235:genetics*; D009994:Osmolar Concentration; D010902:Pituitary Gland / Q000378:metabolism; D010907:Pituitary Hormones / Q000097:blood / Q000378:metabolism; D011388:Prolactin / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D012098:Reproduction; D012756:Sheep
D006062:Gonadotropins; D010907:Pituitary Hormones; D012333:RNA, Messenger; D011388:Prolactin; D013006:Growth Hormone