Differential BrdU uptake in 3 cell cycles and the resultant 3-way sister-chromatid differentiation at M3 endoreduplicated chromosome level--a hypothesis.
The M3 endoreduplicated chromosomes account for SCE1-3 in a compact form after 3-way sister-chromatid differentiation (3-way SCD). However, a difficulty is faced in the analysis and interpretation of these results. Keeping this in view, the present work attempts to explain a number of possibilities correlating the SCD patterns to the probable patterns of uptake of bromodeoxyuridine (BrdU) over 3 successive cell cycles in M3 endoreduplicated chromosomes. This has been done to facilitate understanding of the staining patterns which could be obtained in the M3 endoreduplicated chromosomes after 3-way SCD, and further for the speedy analysis of such chromosomes, especially in scoring SCE1-3 precisely.
Bamezai
R
R
Department of Anatomy, Banaras Hindu University, Varanasi, U.P., India.
D001973:Bromodeoxyuridine; D002453:Cell Cycle; D006801:Humans; D066298:In Vitro Techniques; D008962:Models, Theoretical; D012854:Sister Chromatid Exchange
D001973:Bromodeoxyuridine