Characterization of the glycosphingolipids of pig cortical bone and cartilage.
Neutral glycosphingolipids and gangliosides were extracted from pig cortical bone and cartilage. To ensure the completeness of extraction, the cortical bone was demineralized and reextracted. Globotriaosylceramide and globoside were noted to be present at high content in the cortical bone. It contained glucosylceramide, lactosylceramide, globotriaosylceramide and globoside as neutral glycosphingolipids at a ratio of 1:0.7:3.1:2.7. In articular cartilage, the ratio was 1:0.7:0.4:0.8. GM3 and GD3 were the major gangliosides in both these tissues. GM3, GM1, GD3, GD1 and GT1 were present at ratios of 1:0.9:0.9:0.1:0.1 in the cortical bone and 1:0:1.2:0.06:0.02 in the cartilage. Neutral glycosphingolipids could be extracted from the cortical bone without the need for demineralization, while most of the gangliosides were extracted after this treatment, implying the occurrence of interactions between gangliosides and minerals in the bone.
Fukaya
N
N
Laboratory of Glycoconjugate Research, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Ito
M
M
Iwata
H
H
Yamagata
T
T
D000818:Animals; D001842:Bone and Bones / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D002358:Cartilage, Articular / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer / Q000379:methods; D005227:Fatty Acids / Q000032:analysis; D006026:Glycoside Hydrolases; D006028:Glycosphingolipids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013552:Swine
D002241:Carbohydrates; D005227:Fatty Acids; D006028:Glycosphingolipids; D006026:Glycoside Hydrolases; C050427:endoglycoceramidase