Synthesis of phosphatidylcholine and phosphatidylglycerol in rat lung mitochondria.
The mitochondrial fraction of adult rat lung contains choline phosphotransferase (EC 2.7.8.2) activity which can not be explained by microsomal contamination estimated on the basis of marker enzyme distribution. Mitochondrial (14C)glycerol-3-phosphate incorporation into PC (phosphatidylcholine) can be distinguished from the microsomal incorporation by different sensitivity to N-ethylmaleimide inhibition. The data indicate that rat lung mitochondria have the intrinsic capability to synthesize PC. Both synthesis of PC and PG (phosphatidylglycerol) are susceptible to isotonic tryptic attack against the cytoplasmic face of isolated rat lung mitochondria, suggesting the outer membrane location of crucial activities involved in the formation of these phospholipids. Rat liver mitochondria are different from rat lung mitochondria with respect to their capability to synthesize PC, their rate of (14C)glycerol-3-phosphate incorporation into PG as well as the submitochondrial site of PG formation.
Schlame
M
M
Institute of Pathological and Clinical Biochemistry, Charite Hospital, Humboldt University, Berlin, GDR.
Rüstow
B
B
Kunze
D
D
D000818:Animals; D002250:Carbon Radioisotopes; D005033:Ethylmaleimide / Q000494:pharmacology; D005994:Glycerophosphates / Q000378:metabolism; D007700:Kinetics; D008168:Lung / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010715:Phosphatidylglycerols / Q000096:biosynthesis*; D051381:Rats; D013367:Submitochondrial Particles / Q000378:metabolism
D002250:Carbon Radioisotopes; D005994:Glycerophosphates; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; C029620:alpha-glycerophosphoric acid; D005033:Ethylmaleimide