Pulsed-field gel electrophoresis determination of the genome size of obligate intracellular bacteria belonging to the genera Chlamydia, Rickettsiella, and Porochlamydia.
The chromosome length of obligate intracellular procaryotes was determined by pulsed-field gel electrophoresis of intact or NotI- and SfiI-restricted genomes. Sizes averaged 2,100, 1,720, 1,550, 2,650, and 1,450 kilobases for Rickettsiella grylli, Rickettsiella melolonthae, Porochlamydia buthi, Porochlamydia chironomi, and Chlamydia psittaci and Chlamydia trachomatis, respectively. An SfiI restriction map of the R. melolonthae genome was derived.
Frutos
R
R
Laboratoire de Pathologie Comparée, Université des Sciences et Techniques du Languedoc, France.
Pages
M
M
Bellis
M
M
Roizes
G
G
Bergoin
M
M
D002689:Chlamydia / Q000235:genetics*; D002692:Chlamydia trachomatis / Q000235:genetics; D002691:Chlamydophila psittaci / Q000235:genetics; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods; D008970:Molecular Weight; D015183:Restriction Mapping; D012287:Rickettsiaceae / Q000235:genetics*; D013045:Species Specificity
D004269:DNA, Bacterial