A previously reported polymorphic plasma protein of dogs and horses, identified as apolipoprotein A-IV.
By using immunoblotting with antiserum specific to human plasma apolipoprotein A-IV (apoA-IV), a previously reported polymorphic plasma protein of dogs viz postalbumin-2 (Pa2) and one of horses viz serum protein 2 (SP2), were identified as apoA-IV of these species. This along with earlier published results implied that: (1) both dog and horse show a high degree of polymorphism at the APOA4 locus with three common alleles in each of the two species; and (2) apoA-IV phenotyping in these two species can be done by analysing plasma/serum samples by a simple method of two-dimensional electrophoresis, conducted under non-denaturing conditions, followed by general-protein staining of gels.
Juneja
R K
RK
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
Gahne
B
B
Lukka
M
M
Ehnholm
C
C
D000818:Animals; D001054:Apolipoproteins A / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D004285:Dogs / Q000097:blood* / Q000276:immunology; D006736:Horses / Q000097:blood* / Q000276:immunology; D011110:Polymorphism, Genetic
D001054:Apolipoproteins A; C022205:apolipoprotein A-IV