Effect of splenectomy on hepasor treatment in allyl-alcohol-traumatized rat liver.
Experimental liver injury was provoked in test rats with and without spleen intraperitoneally with allyl alcohol injections. The rats without spleen were used for tests 2 months after the splenectomy. Traumatized rats received further intraperitoneal injections of Hepasor, a protoberberine alkaloid mixture from Enantia chlorantha (Annonaceae). Biochemical assays from blood plasma, serum alanine transferase, serum alkaline phosphatase, serum creatinine, serum hydroxyproline and serum calcium were done and the total amount of blood obtained by decapitation was measured. Liver and kidney samples for histological processing were taken. The biochemical results obtained show significant changes in serum hydroxyproline which increases cumulatively due to traumatization, Hepasor treatment and splenectomy. In case of spenectomy, the absolute volume of circulating blood enhanced under Hepasor treatment. The histological findings in the liver sections show that a 2-week Hepasor therapy of the 2-week pretraumatized rats greatly furthers the healing process during prolonged traumatization. The preventive effect of Hepasor was seen as a diminished occurrence of Kupffer cells, improved cell architecture and promoted mitotic activity. The sedative effect of Hepasor was pivotally evaluated, when massive intra- and extracellular damages were provoked with allyl alcohol in splenectomized rats. This indicate the high regeneration potency of Hepasor on experimentally provoked liver dysplasia.
Virtanen
P
P
Institute of Dentistry, University of Turku, Finland.
Lassila
V
V
Njimi
T
T
Mengata
D E
DE
D000433:1-Propanol / Q000008:administration & dosage / Q000633:toxicity; D000818:Animals; D001600:Berberine Alkaloids / Q000627:therapeutic use*; D001706:Biopsy; D056486:Chemical and Drug Induced Liver Injury; D004338:Drug Combinations / Q000627:therapeutic use; D005260:Female; D007274:Injections, Intraperitoneal; D008107:Liver Diseases / Q000188:drug therapy / Q000473:pathology; D020005:Propanols; D051381:Rats; D013156:Splenectomy
D001600:Berberine Alkaloids; D004338:Drug Combinations; D020005:Propanols; C055787:hepasor; C006463:allyl alcohol; D000433:1-Propanol