title: Glyceraldehyde-3-phosphate dehydrogenase is a microtubule binding protein in a human colon tumor cell line.
abstract: A protein which binds to tubulin polymer was isolated from a human colonic tumor cell line. This protein has a molecular mass of 35 kDa, as determined by polyacrylamide slab gel electrophoresis. The protein was purified by affinity chromatography on taxol-stabilized microtubules, and it did not cross-react with anti-MAP2 or anti-tau antibodies. This protein was identified as glyceraldehyde-3-phosphate dehydrogenase by its enzyme activity and immunoblotting experiments. The purified protein caused a pronounced enhancement in the turbidity increase produced by in vitro tubulin polymerization, and electron microscopic observations revealed the presence of bundles of microtubules.
authors.affiliation: INSERM U 61, Unité de Biologie Cellulaire et de Physiopathologie Digestives, Strasbourg, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Launay
authors.forename: A
authors.initials: A
authors.lastname: Jellali
authors.forename: M T
authors.initials: MT
authors.lastname: Vanier
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002846:Chromatography, Affinity; D003110:Colonic Neoplasms / Q000201:enzymology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification*; D008870:Microtubules / Q000201:enzymology* / Q000648:ultrastructure; D008970:Molecular Weight; D011108:Polymers; D051381:Rats; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D008869:Microtubule-Associated Proteins; D011108:Polymers; D014404:Tubulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases