Effect of HEPES on the taurine uptake by cultured glial cells.
HEPES inhibited the taurine uptake in glial cells. A different kind of inhibition was observed when HEPES was present in the culture medium or in the incubation medium used for the taurine-uptake measurement. As an example of a possible interference of HEPES in pharmacological experiments, we have studied the effect of this buffer on the modulation of taurine uptake by beta agonists or ionic concentration.
Lleu
P L
PL
Centre de Neurochimie du CNRS et Unité 44 de l'INSERM, Strasbourg, France.
Rebel
G
G
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D046508:Culture Techniques / Q000379:methods; D006531:HEPES / Q000494:pharmacology*; D007700:Kinetics; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology*; D013654:Taurine / Q000378:metabolism*
D010879:Piperazines; D013654:Taurine; D006531:HEPES