Crossed immunoelectrophoretic analysis of Flavobacterium meningosepticum DNA hybridization groups I and II.
By means of crossed immunoelectrophoresis and crossed-line immunoelectrophoresis, antigens were compared from four strains and five strains, respectively, of Flavobacterium meningosepticum DNA hybridization groups I and II. Two reference antisera raised against the pooled antigens of each of the two DNA groups were used. The two reference antigen-antibody systems produced 81 and 51 immunoprecipitates, respectively, and within each group nearly all antigens were common to all strains, giving matching coefficients of 0.98-1.00. Group specific antigens were not detected. When antigens from individual strains from each group were compared to individual strains of the other group, however, 5-15 of the antigens were not cross-reactive, giving matching coefficients of 0.83-0.94. The antigenic relatedness between strains belonging to the two different groups was, therefore, just below the level commonly found for strains belonging to the same species. The results thus confirm the previously published differences detected by a DNA hybridization technique.
Høiby
N
N
Statens Seruminstitut, Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Bruun
B
B
D000942:Antigens, Bacterial / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000276:immunology; D007123:Immunoelectrophoresis, Two-Dimensional; D012689:Sequence Homology, Nucleic Acid
D000942:Antigens, Bacterial; D004269:DNA, Bacterial