title: Screening and confirmation of thyreostatics in urine by gas chromatography with nitrogen-phosphorus detection and gas chromatography-mass spectrometry after sample clean-up with a mercurated affinity column.
abstract: Methods are described for the screening and confirmation of residues of the thyreostatics thiouracil, methylthiouracil and propylthiouracil in urine samples of cattle at levels down to 25 micrograms/l. After a selective preconcentration of the thiol-containing thyreostatics on a mercurated affinity column, the analytes are derivatized by extractive alkylation and analysed by gas chromatography with nitrogen-phosphorus or mass spectrometric detection.
authors.affiliation: State Institute for Quality Control of Agricultural Products (RIKILT), Wageningen, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Schilt
authors.forename: J M
authors.initials: JM
authors.lastname: Weseman
authors.forename: H
authors.initials: H
authors.lastname: Hooijerink
authors.forename: H J
authors.initials: HJ
authors.lastname: Korbee
authors.forename: W A
authors.initials: WA
authors.lastname: Traag
authors.forename: M J
authors.initials: MJ
authors.lastname: van Steenbergen
authors.forename: W
authors.initials: W
authors.lastname: Haasnoot
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D008713:Methimazole / Q000378:metabolism* / Q000652:urine; D008779:Methylthiouracil / Q000652:urine; D011441:Propylthiouracil / Q000652:urine; D013889:Thiouracil / Q000031:analogs & derivatives / Q000652:urine*
chemical_list: C060320:6-phenylthiouracil; D008713:Methimazole; D013889:Thiouracil; D011441:Propylthiouracil; D008779:Methylthiouracil