The effect of alkaline phosphatase on platelet aggregation.
Elevated concentrations of alkaline phosphatase occur in a variety of diseases. The effect of alkaline phosphatase on platelet aggregation was tested. Adenosine diphosphate-induced aggregation was inhibited at the highest concentrations of alkaline phosphatase employed. Similar results were observed when epinephrine, collagen and arachidonic acid were used as aggregating agents. A dose-dependent inhibition of ristocetin-induced aggregation by alkaline phosphatase was observed. These results were confirmed using plasma from patients with cholestatic disease and elevated concentrations of alkaline phosphatase similar to those used in the in vitro studies. These data indicate that patients with significantly elevated levels of alkaline phosphatase may be predisposed to a thrombocytopathy.
Weitberg
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AB
Division of Haematology/Oncology, Roger Williams Cancer Center, Providence, Rhode Island 02908.
D000244:Adenosine Diphosphate / Q000502:physiology; D000469:Alkaline Phosphatase / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors; D012310:Ristocetin / Q000494:pharmacology
D010975:Platelet Aggregation Inhibitors; D012310:Ristocetin; D000244:Adenosine Diphosphate; D000469:Alkaline Phosphatase