title: Studies on the irreversible step of pepsinogen activation.
abstract: The bond cleavage step of pepsinogen activation has been investigated in a kinetic study in which the denatured products of short-term acidifications were separated on SDS-polyacrylamide gels and the peptide products were quantitated by densitometry. Although several peptide products were observed, under the conditions of the experiments (pH values between 2.0 and 2.8, 22 degrees C), the only one that was a product of an initial bond cleavage was the 44-residue peptide, which upon removal from pepsinogen yields pepsin. The rate constant for this bond cleavage is 0.015 s-1 at pH 2.4, which is the same as that at which the alkali-stable potential activity of pepsinogen had been found to convert to the alkali-labile activity of pepsin. When the conversion of zymogen to enzyme was followed by the change in fluorescence of adsorbed 6-(p-toluidinyl)naphthalene-2-sulfonate (TNS), the rate of change in TNS fluorescence was the same as the conversion to alkali lability. However, pepstatin blocked the bond cleavage of pepsinogen to pepsin, but it permitted the fluorescence change to proceed. In fact, it accelerated the apparent rate of change of TNS fluorescence by shifting the pKa of an essential conjugate acid from 1.7 to 2.6. The conversion to alkali lability, therefore, may be considered to be a composite of a relatively slow conformational change (at the measured rate), followed immediately by a relatively fast bond cleavage.
authors.affiliation: Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226.
authors.forename: D M
authors.initials: DM
authors.lastname: Glick
authors.forename: Y
authors.initials: Y
authors.lastname: Shalitin
authors.forename: C R
authors.initials: CR
authors.lastname: Hilt
mesh_terms: D002151:Calorimetry; D004789:Enzyme Activation; D005456:Fluorescent Dyes; D007700:Kinetics; D008433:Mathematics; D008962:Models, Theoretical; D009282:Naphthalenesulfonates; D010435:Pepsinogens / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D009282:Naphthalenesulfonates; D010435:Pepsinogens; C009497:2-(4-toluidino)-6-naphthalenesulfonic acid