title: Protein-RNA interactions in an icosahedral virus at 3.0 A resolution.
abstract: Nearly 20 percent of the packaged RNA in bean-pod mottle virus (BPMV) binds to the capsid interior in a symmetric fashion and is clearly visible in the electron density map. The RNA displaying icosahedral symmetry is single-stranded with well-defined polarity and stereochemical properties. Interactions with protein are dominated by nonbonding forces with few specific contacts. The tertiary and quaternary structures of the BPMV capsid proteins are similar to those observed in animal picornaviruses, supporting the close relation between plant comoviruses and animal picornaviruses established by previous biological studies.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Stauffacher
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: T
authors.initials: T
authors.lastname: Schmidt
authors.forename: W
authors.initials: W
authors.lastname: Bomu
authors.forename: G
authors.initials: G
authors.lastname: Kamer
authors.forename: M
authors.initials: M
authors.lastname: Shanks
authors.forename: G
authors.initials: G
authors.lastname: Lomonossoff
authors.forename: J E
authors.initials: JE
authors.lastname: Johnson
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000378:metabolism* / Q000648:ultrastructure; D003461:Crystallography; D004577:Electron Probe Microanalysis; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000032:analysis* / Q000235:genetics / Q000648:ultrastructure; D010942:Plant Viruses / Q000032:analysis* / Q000235:genetics / Q000648:ultrastructure; D011487:Protein Conformation; D012367:RNA, Viral / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D046911:Macromolecular Substances; D012367:RNA, Viral