title: Intracellular recording from putative dopamine-containing neurons in the ventral tegmental area of Tsai in a brain slice preparation.
abstract: Coronal slices of rat mesencephalon containing the ventral tegmental area of Tsai (VTA) and the substantia nigra were prepared. Stable intracellular recordings were obtained from presumed dopamine (DA)-containing neurons in the VTA. Both silent and spontaneously active cells were encountered; spontaneously active neurons fired in an extremely regular pacemaker-like fashion. These neurons had resting membrane potentials ranging from -45 to -75 mV and input resistances ranging from 80-400 M omega. DA-containing neurons in the VTA demonstrated marked anomalous rectification in response to hyperpolarizing current pulses. Application of DA or the GABAB agonist, baclofen, to the bathing medium produced suppression of spontaneous firing, sometimes accompanied by membrane hyperpolarization. Neuronal input resistance was not changed consistently by DA and was generally reduced by baclofen.
authors.affiliation: Neuroscience Research Division, Abbott Laboratories, Abbott Park, IL 60064.
authors.forename: A L
authors.initials: AL
authors.lastname: Mueller
authors.forename: M S
authors.initials: MS
authors.lastname: Brodie
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D004298:Dopamine / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008867:Microtomy; D051381:Rats; D011919:Rats, Inbred Strains; D013681:Tegmentum Mesencephali / Q000187:drug effects / Q000502:physiology*
chemical_list: D001418:Baclofen; D004298:Dopamine