Circulating factors that modify lung cell DNA synthesis following exposure to inhaled oxidants. I. Effect of serum and lavage on lung fibroblasts following exposure of adult rats to 1 ppm ozone.
Adult rats were exposed to 1 ppm (1.96 mg/m3) ozone or air for 2 wk. Animals were sacrificed at 3, 5, 7, or 14 d after the onset of exposure, and samples of plasma and lung lavage were obtained. Heat-inactivated plasma, from animals exposed to ozone for 7 or 14 d, significantly increased DNA synthesis by lung fibroblasts compared with plasma from air-exposed animals. Fractionation of plasma and lavage samples indicated that the factor responsible had an isoelectric point of 6.45-6.75 and a molecular weight of 32 +/- 2 kDa. This factor has a dose-dependent effect on lung fibroblast DNA synthesis in culture, but no significant effect on cultured pneumocyte DNA synthesis. The factor is detectable within 72 h of exposure, and may hold some promise as a marker of early oxidant lung injury.
Tanswell
A K
AK
Department of Paediatrics, University of Western Ontario, London, Canada.
Fraher
L J
LJ
Grose
E C
EC
D000818:Animals; D001273:Atmosphere Exposure Chambers; D001681:Biological Assay; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D004781:Environmental Exposure; D005346:Fibroblast Growth Factors / Q000032:analysis* / Q000097:blood; D005347:Fibroblasts / Q000378:metabolism; D007525:Isoelectric Focusing / Q000379:methods; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010126:Ozone / Q000633:toxicity*; D051381:Rats
D003470:Culture Media; D005346:Fibroblast Growth Factors; D010126:Ozone; D004247:DNA