Implantation of vascular grafts lined with genetically modified endothelial cells.
The possibility of using the vascular endothelial cell as a target for gene replacement therapy was explored. Recombinant retroviruses were used to transduce the lacZ gene into endothelial cells harvested from mongrel dogs. Prosthetic vascular grafts seeded with the genetically modified cells were implanted as carotid interposition grafts into the dogs from which the original cells were harvested. Analysis of the graft 5 weeks after implantation revealed genetically modified endothelial cells lining the luminal surface of the graft. This technology could be used in the treatment of atherosclerosis disease and the design of new drug delivery systems.
Wilson
J M
JM
Whitehead Institute, Cambridge, MA.
Birinyi
L K
LK
Salomon
R N
RN
Libby
P
P
Callow
A D
AD
Mulligan
R C
RC
D000818:Animals; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries / Q000601:surgery; D002478:Cells, Cultured; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D005822:Genetic Vectors; D012190:Retroviridae / Q000235:genetics; D014162:Transfection