title: Alterations in cardiac sarcoplasmic reticulum calcium transport in the postischemic "stunned" myocardium.
abstract: This study examined the possibility that the postischemic mechanical depression observed in the "stunned" myocardium is a result of an alteration in the control of intracellular calcium. Regional myocardial stunning was produced in five open-chest dogs by eight to twelve 5-minute occlusions of the left anterior descending coronary artery, alternated with 10-minute reflow periods and followed by a final 60-minute period of reperfusion. Systolic segment shortening in the postischemic zone, measured by sonomicrometry, fell from 14.9% at baseline to -1.1% at the end of reperfusion. Sarcoplasmic reticulum isolated from stunned myocardium demonstrated a 17% reduction in oxalate-supported 45Ca2+ transport compared with sarcoplasmic reticulum from normal myocardium (0.93 vs. 1.12 mumol Ca2+/mg protein/min, p less than 0.005). There was also a 20% decrease in the maximal activation by Ca2+ of the sarcoplasmic reticulum Ca2+, Mg2+-ATPase (2.46 vs. 1.96 mumol Pi/mg protein/min, p less than 0.005), and a downward shift in the Ca2+-activation curve of the Ca2+, Mg2+-ATPase. These results indicate that myocardial stunning is associated with damage to the calcium-transport system of the sarcoplasmic reticulum. Altered intracellular control may contribute to the inability of the stunned heart to maintain normal mechanical function.
authors.affiliation: Department of Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland.
authors.forename: S M
authors.initials: SM
authors.lastname: Krause
authors.forename: W E
authors.initials: WE
authors.lastname: Jacobus
authors.forename: L C
authors.initials: LC
authors.lastname: Becker
mesh_terms: D000818:Animals; D001692:Biological Transport; D001696:Biomechanical Phenomena; D002118:Calcium / Q000378:metabolism*; D004285:Dogs; D006439:Hemodynamics; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
chemical_list: D002118:Calcium