Specific amplification of the ZFY gene to screen sex in man.
Using the polymerase chain reaction, a sequence comprising 400bp of the human ZFY gene was amplified specifically in the male. The method allows detection of the presence of the ZFY gene in the order of 1:10(4) cells.
Ebensperger
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Institut für Humangenetik und Anthropologie der Universität, Freiburg, Federal Republic of Germany.
Studer
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Epplen
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JT
D015139:Blotting, Southern; D015342:DNA Probes; D004268:DNA-Binding Proteins; D004259:DNA-Directed DNA Polymerase; D005260:Female; D005784:Gene Amplification; D012739:Gonadal Steroid Hormones / Q000235:genetics*; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008297:Male; D012732:Sex Determination Analysis; D014157:Transcription Factors; D014998:Y Chromosome
D015342:DNA Probes; D004268:DNA-Binding Proteins; D012739:Gonadal Steroid Hormones; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C054430:ZFY protein, human; D004259:DNA-Directed DNA Polymerase