Turnover and compartmentation of gp70/p15 E in psi 2 cells.
The distribution of Moloney murine leukemia virus gp70/p15 E between cell surface and intracellular compartments and the kinetics of transfer between these compartments was examined in psi 2 cells. A novel biotin derivatization and recovery assay was used to quantitate pulse-labeled protein accessible at 4 degrees C (cell surface), 18 degrees C (cell surface and an intracellular compartment), or inaccessible at any temperature. Cell surface (4 degrees C) gp70 and p15 E turn over more rapidly than intracellular pools of these proteins. The decrease in cell surface gp70 and p15 E after one hour of chase is accounted for by an increase in that which is inaccessible to biotinyl reagent.
Hare
J F
JF
Department of Biochemistry, Oregon Health Sciences University, Portland 97201.
D000818:Animals; D002451:Cell Compartmentation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism*
D008562:Membrane Glycoproteins; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins