title: Measurement of urinary somatomedin C by a chemiluminescent method.
abstract: We present a method for the measurement of the total Somatomedin C (SmC) content in human early morning urine samples after dialysis, extraction, and concentration. We modified a chemiluminescence immunoassay, previously developed for SmC determination in serum, for analysis of SmC in urine. Appropriate sensitivity was obtained by the preparation of a new chemiluminescent tracer (AEEI-COOH-SmC) and the optimization of a competitive non-equilibrium immunoassay system which had a detection limit of 0.24 fmol SmC per tube.
authors.affiliation: Department of Clinical Physiopathology University of Florence, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Orlando
authors.forename: B
authors.initials: B
authors.lastname: Tode
authors.forename: P
authors.initials: P
authors.lastname: Pinzani
authors.forename: F
authors.initials: F
authors.lastname: Bassi
authors.forename: G
authors.initials: G
authors.lastname: Messeri
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000652:urine*; D008163:Luminescent Measurements; D011863:Radioimmunoassay; D013002:Somatomedins / Q000652:urine*
chemical_list: D002352:Carrier Proteins; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I