Characterization of ribonucleolytic activity of angiogenin towards tRNA.
Yeast tRNA is a convenient substrate for the assay of the ribonucleolytic activity of human angiogenin. The optimal pH, [NaCl], and temperature for tRNA cleavage by angiogenin are approximately 6.8, 15-30 mM, and approximately 55 degrees C, respectively, as compared with approximately 8.0, 100-200 mM, and approximately 65 degrees C, respectively, for RNase A. Polyanions and metals both inhibit angiogenin and RNase A but to different extents.
Lee
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Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115.
Vallee
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D043925:Angiogenesis Inducing Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D006224:Cricetinae; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010926:Placental Hormones / Q000494:pharmacology; D011131:Polyribonucleotides / Q000494:pharmacology; D011506:Proteins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism* / Q000502:physiology; D012259:Ribonuclease, Pancreatic / Q000037:antagonists & inhibitors; D013696:Temperature
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D010926:Placental Hormones; D011131:Polyribonucleotides; D011506:Proteins; D012335:RNA, Ribosomal; C022052:placental ribonuclease inhibitor; D012343:RNA, Transfer; C046366:angiogenin; D012259:Ribonuclease, Pancreatic