Structural domains and conformational changes in nuclear chromatin: a quantitative thermodynamic approach by differential scanning calorimetry.
A good deal of information on the thermodynamic properties of chromatin was derived in the last few years from optical melting experiments. The structural domains of the polynucleosomal chain, the linker, and the core particle denature as independent units. The differential scanning calorimetry profile of isolated chromatin is made up of three endotherms, at approximately 74, 90, and 107 degrees C, having an almost Gaussian shape. Previous work on this matter, however, was mainly concerned with the dependence of the transition enthalpy on external parameters, such as the ionic strength, or with the melting of nuclei from different sources. In this paper we report the structural assignment of the transitions of rat liver nuclei, observed at 58, 66, 75, 92, and 107 degrees C. They are representative of the quiescent state of the cell. The strategy adopted in this work builds on the method developed for the investigation of complex biological macromolecules. The heat absorption profile of the nucleus was related to the denaturation of isolated nuclear components; electron microscopy and electrophoretic techniques were used for their morphological and molecular characterization. The digestion of chromatin by endogenous nuclease mimics perfectly the decondensation of the higher order structure and represented the source of several misinterpretations. This point was carefully examined in order to define unambiguously the thermal profile of native nuclei. The low-temperature transitions, centered around 58 and 66 degrees C, arise from the melting of scaffolding structures and of the proteins associated with heterogeneous nuclear RNA.(ABSTRACT TRUNCATED AT 250 WORDS)
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Centro di Studi Chimico-Fisici di Macromolecole Sintetiche e Naturali, CNR, Genova, Italy.
Abelmoschi
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ML
Gogioso
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L
Parodi
S
S
Barboro
P
P
Cavazza
B
B
Patrone
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D000818:Animals; D002152:Calorimetry, Differential Scanning; D002455:Cell Division; D002843:Chromatin / Q000302:isolation & purification / Q000648:ultrastructure*; D008099:Liver / Q000648:ultrastructure; D008297:Male; D008836:Micrococcal Nuclease; D008854:Microscopy, Electron; D015394:Molecular Structure; D011487:Protein Conformation; D011489:Protein Denaturation; D051381:Rats; D011919:Rats, Inbred Strains; D013816:Thermodynamics
D002843:Chromatin; D008836:Micrococcal Nuclease