title: Comparison of the enzymatic composition of cell-free extracts of non-aflatoxigenic Aspergillus parasiticus with respect to late stages of aflatoxin biosynthesis.
abstract: Cell-free extracts of fungal mycelia of two non-aflatoxigenic isolates of Aspergillus parasiticus (SRRC 163 and SRRC 2043) were examined for enzyme activities involved in the latter stages of aflatoxin biosynthesis. Post-microsomal fractions (105 Kxg supernatant) of both SRRC 163 (ATTC 56774) and SRRC 2043 were able to convert sterigmatocystin (ST) to O-methylsterigmatocystin (OMST); whereas the microsomal (105 Kxg pellet) preparation of only SRRC 163 was able to convert OMST to aflatoxin B1 (AFB1). A mixture of the microsomal fraction of SRRC 163 and post-microsomal fraction of SRRC 2043 converted ST to AFB1. Electrophoretic protein separations of the microsomal fractions of the two A. parasiticus isolates on non-denaturing or denaturing polyacrylamide gel electrophoresis (PAGE) provided equivalent protein profiles.
authors.forename: D
authors.initials: D
authors.lastname: Bhatnagar
authors.forename: T E
authors.initials: TE
authors.lastname: Cleveland
authors.forename: A R
authors.initials: AR
authors.lastname: Lax
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis*; D001230:Aspergillus / Q000201:enzymology*; D002458:Cell Fractionation; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel
chemical_list: D000348:Aflatoxins