Interactions of the lipopeptide antifungal iturin A with lipids in mixed monolayers.
The miscibility and the interactions of the antifungal lipopeptide iturin A with lipids, DMPC and cholesterol, are studied in monolayers at the air/water interface and a comparison of the respective behaviour of iturin A and the biologically inactive methylated derivative MeTyr-iturin A is made. Each lipopeptide is miscible with anyone of the lipids. This behaviour is revealed by the dependence of the transition pressure upon composition and by deviations from the additivity rule of the mean molecular area. The thermodynamic properties of the mixed systems are studied by the method of Goodrich. The mixed monolayers are always more stable than the two separate components, subsequently there are interactions between the components. However, the excess free energy of mixing delta Gexm is positive for the iturin A/DMPC system which is an indication that the interactions between lipopeptide and lipid molecules are weaker than the interactions between the pure components themselves. This is compatible with the presence of self-associated lipopeptide molecules. However, delta Gexm is highly negative for the iturin A/cholesterol system giving evidence of the formation of a specific complex between iturin A and cholesterol which is not the case with the methylated derivative. These data are analysed in connection with previous results concerning the pore-forming properties of these lipopeptides and the lack of biological activity of MeTyr-iturin A.
Maget-Dana
R
R
Centre de Biophysique Moléculaire, C.N.R.S., Orléans, France.
Harnois
I
I
Ptak
M
M
D000900:Anti-Bacterial Agents; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine; D008563:Membrane Lipids; D008745:Methylation; D010455:Peptides; D010456:Peptides, Cyclic; D012995:Solubility; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D000900:Anti-Bacterial Agents; D008563:Membrane Lipids; D010455:Peptides; D010456:Peptides, Cyclic; C013579:iturin A; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine