Release of PI-anchoring enzymes and other effects of phosphatidylinositol-specific phospholipase C from Bacillus thuringiensis on TN-368 cells from a moth ovary.
Release of PI-anchoring enzymes and other effects of phosphatidylinositol-specific phospholipase C from Bacillus thuringiensis on TN-368 cells from a moth ovary. Toxicon 27, 637-645, 1989.--The effect of phosphatidylinositol-specific phospholipase C(PIPLC) from Bacillus thuringiensis was investigated on TN-368 cells, derived from the ovary of a moth, Trichoplusia ni. Quantitative analysis of lipids showed that phosphatidylinositol (PI) was contained as one of the major phospholipids in TN-368 cells, whereas sphingomyelin and cholesterol were minor lipid components. When TN-368 cells were treated with PIPLC, significant amounts of alkaline phosphatase, 5'-nucleotidase and beta-glucosidase were released from these cells. Thus, these enzymes were shown to be PI-anchoring proteins in the plasma membrane of these cells. In the presence of 4.2 units of PIPLC, the cell growth of TN-368 was inhibited by 50%. In contrast with normal cells, the cells cultured in the presence of PIPLC became swollen and globular, losing their protoplasmic extensions. Also, there was degeneration of the interior of TN-368 cells cultivated in the presence of PIPLC. Mitochondria became swollen with a decrease in number of granules while the crista turned transparent. Also, an increase in lysosomes was observed and vacuoles seemingly derived from smooth endoplasmic reticula appeared.
Ikezawa
H
H
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Hashimoto
A
A
Taguchi
R
R
Nakabayashi
T
T
Himeno
M
M
D000818:Animals; D001413:Bacillus thuringiensis / Q000201:enzymology*; D002460:Cell Line; D005260:Female; D008854:Microscopy, Electron; D009036:Moths; D010053:Ovary / Q000166:cytology / Q000187:drug effects; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D010738:Type C Phospholipases / Q000633:toxicity*
D010716:Phosphatidylinositols; D010743:Phospholipids; D010738:Type C Phospholipases