Secondary structure analysis of tubulin and microtubules with Raman spectroscopy.
Raman spectroscopy is used to study the secondary structure of tubulin in the assembled and the dissociated states from the analysis of the amide-I band. Essentially two states are recognized: the GTP- and the GDP-bound state, differing in alpha-helix and antiparallel beta-sheet content. Microtubules give a spectrum which is very similar to the GDP-bound state. MAPs and temperature have minor effects, while increasing the pH up to 8 causes a reduction in alpha-helix content and a increase in antiparallel beta-sheet. The binding of demecolcine also induces structural changes which are similar to the GDP-bound state.
Audenaert
R
R
Laboratory of Chemical and Biological Dynamics, University of Leuven, Belgium
Heremans
L
L
Heremans
K
K
Engelborghs
Y
Y
D000577:Amides; D000818:Animals; D003703:Demecolcine / Q000494:pharmacology; D006153:Guanosine Diphosphate / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008869:Microtubule-Associated Proteins / Q000494:pharmacology; D008870:Microtubules / Q000648:ultrastructure*; D011108:Polymers; D011485:Protein Binding; D013059:Spectrum Analysis, Raman; D013552:Swine; D013696:Temperature; D014404:Tubulin / Q000648:ultrastructure*
D000577:Amides; D046911:Macromolecular Substances; D008869:Microtubule-Associated Proteins; D011108:Polymers; D014404:Tubulin; D006153:Guanosine Diphosphate; D003703:Demecolcine