[Effects of cytokine on expression of tumor antigen TA-4 in squamous cell carcinoma].
The effects of peripheral mononuclear cells on the production of TA-4 were studied using SKG-IIIa cells, an established cell line of squamous cell carcinoma. The mononuclear cells were isolated from the heparinized peripheral blood from healthy donors and patients with uterine cancer, and were treated with Con A for 3 days. The supernatant of Con A treated mononuclear cells significantly increased TA-4 release from SKG-IIIa cells, but also induced apparent cytotoxicity. The cellular TA-4 content, which was determined by the specific RIA or by immunohistochemical staining using TA-4 antibodies, was also increased by the treatment. There was significant difference in stimulating activity from the mononuclear cells in healthy donors and malignant patients. These stimulating factors from the mononuclear cells had molecular weights of 36,000, 61,000 or 82,000 daltons on Sephadex G-100 column chromatography, whereas the cytotoxic activity was eluted in those fractions with molecular weight of 61,000 daltons. These results indicated that the production of TA-4 in squamous cell carcinoma could be stimulated by some factors from the peripheral mononuclear cells.
Kobayashi
M
M
Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine.
D000951:Antigens, Neoplasm / Q000032:analysis / Q000096:biosynthesis*; D001685:Biological Factors / Q000032:analysis / Q000097:blood / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007525:Isoelectric Focusing; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000097:blood
D000951:Antigens, Neoplasm; D001685:Biological Factors; C036649:tumor antigen TA-4, human; D003208:Concanavalin A