Fast detection of homogeneous chemiluminescent immunoassays with a sensitive photoplate.
Chemiluminescent immunoassays performed in microtitre plates can be detected with a sensitive instant photoplate. An apparatus was constructed for this purpose making use of whole polystyrene microtitre plates plates possible. One of the advantages of immunoassays using enzyme-enhanced chemiluminescence is the possibility for developing homogeneous immunoassays. An example is described for a solid-phase immunoassay for 17 beta-testosterone making use of non-specific energy transfer in combination with photoplate detection. A testosterone-peroxidase conjugate was used as label. Quantification was performed using image-analysis techniques. The assay was also performed with colorimetric substrates for comparison.
Jansen
E H
EH
Laboratory for Clinical Chemistry, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Buskens
C A
CA
van den Berg
R H
RH
D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D013739:Testosterone / Q000032:analysis
D013739:Testosterone