title: [Improved method of purification of L-lysine-alpha-oxidase from the fungus Trichoderma sp].
abstract: Relatively rapid procedure was developed for isolation and purification of the antitumor enzyme L-lysine alpha-oxidase. The procedure involved three steps instead of six or four steps described previously, with a yield of 64.4%. The homogenous preparation was obtained as shown by gel electrophoresis and ultracentrifugation. The antitumor activity of the enzyme both in vivo and in vitro is under investigation.
authors.forename: N B
authors.initials: NB
authors.lastname: Iankevich
authors.forename: N F
authors.initials: NF
authors.lastname: Laugalene
authors.forename: V S
authors.initials: VS
authors.lastname: Vesa
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Khaduev
authors.forename: I P
authors.initials: IP
authors.lastname: Smirnova
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D050830:L-Amino Acid Oxidase; D003904:Mitosporic Fungi / Q000201:enzymology*; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase
vernacular_title: Usovershenstvovannaia skhema ochistki L-lizin-alpha-oksidazy griba Trichoderma sp.