title: Measurement of vitamin K in human liver by gradient elution high-performance liquid chromatography using platinum-black catalyst reduction and fluorimetric detection.
abstract: A sensitive and precise method for measuring endogenous phylloquinone (K1) and menaquinone (MK-n) in human liver was developed, based on gradient elution high-performance liquid chromatography using platinum-black catalyst reduction and fluorimetric detection. Subnanogram levels of vitamin K compounds in 1 g of liver specimen were detectable. We measured vitamin K concentrations in 38 human resected livers. K1 and MK-4 to MK-13 were detected. The concentrations of MK-10 to MK-12 in livers with chronic hepatitis (n = 10) and cirrhosis (n = 22) were significantly lower than in normal livers (n = 6). It is suggested that the decreased concentrations indicate functional damage of the hepatocytes.
authors.affiliation: Second Department of Surgery, Kyoto University Faculty of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Usui
authors.forename: N
authors.initials: N
authors.lastname: Nishimura
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Okanoue
authors.forename: M
authors.initials: M
authors.lastname: Kimoto
authors.forename: K
authors.initials: K
authors.lastname: Ozawa
mesh_terms: D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008099:Liver / Q000032:analysis*; D008930:Mitochondria, Liver / Q000032:analysis; D010084:Oxidation-Reduction; D010984:Platinum; D013050:Spectrometry, Fluorescence; D014812:Vitamin K / Q000032:analysis*; D010837:Vitamin K 1 / Q000032:analysis
chemical_list: D014812:Vitamin K; D010984:Platinum; D010837:Vitamin K 1