title: [Aldehydes and ketones in silage: quantitative analysis by high performance liquid chromatography].
abstract: Carbonyl compound toxicity is known in several species but no study has been carried out with ruminants. Such volatile compounds exist in silages. After condensation of aldehydes and ketones with 2,4-dinitrophenylhydrazine, quantitative analysis was performed with 37 silages. It was found that quantities of carbonyl compounds varied from 36 mg/kg of dry matter (DM) to 1,535 mg/kg DM with a mean value of 642 mg/kg DM. Ethanal was 63% of the total amount of carbonyl compounds (mol/kg DM). Other molecules were propanal, propanone, butanal (n- and iso-), butanone and n- and iso- pentanal. The total amount of carbonyl compounds correlated positively with the dry matter percentage and negatively with the pH, crude fiber, ash content and volatile fatty acids. Thus, carbonyl compounds seem to be dependent up on silage storage conditions. Lactic flora could be involved in the synthesis of these compounds.
authors.affiliation: Laboratoire associé INRA de prévention globale, Ecole nationale vétérinaire de Nantes, France.
authors.forename: D
authors.initials: D
authors.lastname: Langin
authors.forename: P
authors.initials: P
authors.lastname: Nguyen
authors.forename: H
authors.initials: H
authors.lastname: Dumon
authors.forename: A
authors.initials: A
authors.lastname: Malek
mesh_terms: D000447:Aldehydes / Q000032:analysis* / Q000633:toxicity; D000821:Animal Feed / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007659:Ketones / Q000032:analysis* / Q000633:toxicity; D012820:Silage / Q000032:analysis* / Q000633:toxicity
chemical_list: D000447:Aldehydes; D007659:Ketones
vernacular_title: Aldéhydes et cétones dans les ensilages: analyse quantitative par chromatographie liquide à haute performance.