Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum.
Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.
Tesoriere
G
G
Institute of Biological Chemistry, University of Palermo, Italy.
Vento
R
R
Calvaruso
G
G
Schiavo
M R
MR
Giuliano
M
M
Taibi
G
G
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D002417:Cattle; D002642:Chick Embryo; D004261:DNA Replication / Q000187:drug effects*; D006131:Growth Inhibitors / Q000494:pharmacology*; D008970:Molecular Weight; D009924:Organ Culture Techniques; D012160:Retina / Q000187:drug effects / Q000378:metabolism*
D001798:Blood Proteins; D006131:Growth Inhibitors