Evaluation of the genotoxicity of domoic acid in a hepatocyte-mediated assay with V79 Chinese hamster lung cells.
Domoic acid, a recognized neurotoxin derived from contaminated samples of the blue mussel (Mytilus edulis L.), was analyzed for mutagenicity at 2 loci and for 2 cytogenetic parameters in a hepatocyte-mediated assay with V79 Chinese hamster lung fibroblasts. Genetic end-points measured were: mutation to 6-thioguanine resistance at the HGPRTase locus; mutation to ouabain resistance at the Na+,K+-ATPase locus; sister-chromatid exchange (SCE) and micronucleus frequency (MN). None of these genetic end-points was significantly affected by exposure to domoic acid at dose levels of 27.2 and 54.4 micrograms/ml with or without activation by freshly isolated rat liver hepatocytes. It was concluded that, within the limits of the test system employed, domoic acid was non-genotoxic to V79 cells.
Rogers
C G
CG
Toxicology Research Division, Food Directorate, Health and Welfare Canada, Ottawa, Ont.
Boyes
B G
BG
D000818:Animals; D001711:Biotransformation; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D005347:Fibroblasts; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D008099:Liver / Q000378:metabolism; D008297:Male; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000493:pharmacokinetics; D009498:Neurotoxins / Q000493:pharmacokinetics / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000187:drug effects
D009153:Mutagens; D009498:Neurotoxins; C012301:domoic acid; D007608:Kainic Acid