A preparation of melittin depleted of phospholipase A2 by ion exchange chromatography in denaturing solvents.
A preparation of melittin depleted of phospholipase and N-formyl-melittin can be obtained by ion exchange fast protein liquid chromatography in a buffer system containing 90% methanol or 4 M urea.
Wille
B
B
Max-Planck-Institut für Biologie, Tübingen, Federal Republic of Germany.
D001514:Bee Venoms / Q000302:isolation & purification*; D002021:Buffers; D002852:Chromatography, Ion Exchange / Q000379:methods*; D008555:Melitten / Q000032:analysis / Q000302:isolation & purification*; D000432:Methanol; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000302:isolation & purification*; D054467:Phospholipases A2; D014508:Urea
D001514:Bee Venoms; D002021:Buffers; D008555:Melitten; D014508:Urea; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D000432:Methanol