Cloning of a hemolysin gene from Leptospira interrogans serovar hardjo.
A DNA fragment encoding both hemolysin and sphingomyelinase C activity was cloned from the pathogenic bacterium Leptospira interrogans serovar hardjo. Initial clones were obtained by screening a genomic library in EMBL3 for hemolytic activity. Both hemolytic and sphingomyelinase C activities were coded for by a 3.9-kilobase BamHI fragment. The hemolysin was expressed from its own promoter in Escherichia coli K-12. Similar DNA sequences were also present in the serovars tarassovi and ballum.
del Real
G
G
Department of Bacteriology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Segers
R P
RP
van der Zeijst
B A
BA
Gaastra
W
W
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification; D005796:Genes; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D007921:Leptospira interrogans / Q000235:genetics*; D014774:Virulence
D004269:DNA, Bacterial; D006460:Hemolysin Proteins