Growth characteristics of Tyzzer's organism in cultured mouse hepatocytes.
The growth characteristics of Tyzzer's organisms in cultured mouse hepatocytes were examined by direct bacterial counting and plaque assay. The viability of propagated bacteria and time required for the maximum titers depended on incubation time and inoculum dose, respectively. Both infectious titers and bacterial counts at optimum harvests were much higher than those obtained from infected mouse livers. Immunofluorescence revealed, after a lag phase of about 2 h, a linear multiplication during a 6 h period.
Kawamura
S
S
Department of Biomedical Science, Faculty of Agriculture, University of Tokyo, Japan.
Mitsuoka
T
T
Fujiwara
K
K
D000818:Animals; D001419:Bacteria / Q000254:growth & development*; D002478:Cells, Cultured; D005260:Female; D008099:Liver / Q000382:microbiology*; D051379:Mice; D008813:Mice, Inbred ICR