High-performance ion-exchange chromatography of oligoribonucleotides using linear and hyperbolic salt gradients.
A method for separation and chain length determination of oligo- and polynucleotides by high-performance anion-exchange chromatography was developed, which allows resolution of individual fragments according to their chain length n, up to n approximately 10 by linear gradient of sodium chloride and up to n approximately 30 by an hyperbolic gradient of this salt. The hyperbolic relationship between n and the salt concentration at which elution of the fragment occurs allows determination of the degree of polymerization of oligo- and polynucleotides with unknown n. The method proposed can be used for estimation of the effective charge of nucleic acids with complex structure.
Tsygankov AYu
A. N. Bakh Institute of Biochemistry, U.S.S.R. Academy of Sciences, Moscow.
Motorin YuA
Wolfson
A D
AD
Kirpotin
D B
DB
Orlovsky
A F
AF
D002851:Chromatography, High Pressure Liquid; D009843:Oligoribonucleotides / Q000032:analysis*; D011061:Poly A / Q000032:analysis; D011072:Poly U / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis
D009843:Oligoribonucleotides; D011061:Poly A; D011072:Poly U; D012343:RNA, Transfer