Reduction of human immunodeficiency virus-infected cells from donor blood by leukocyte filtration.
Several filters for leukocyte removal were evaluated in terms of their ability to reduce the cell-associated human immunodeficiency virus (HIV) load in units of blood either inoculated in vitro with lymphocytes from a chronically infected cell line or collected directly from seropositive donors. Filtration of the experimentally inoculated units of blood resulted in a 5.9 log 10 mean reduction (95% confidence interval:7.4-4.5) of tissue culture infectious units (TCIU) as assayed by end-point titration using the coculture assay. Filtration of the units of blood from anti-HIV positive donors lowered the infectivity by over 2 logs, as detected by the coculture and polymerase chain reaction (PCR) techniques. However, residual cell-associated virus was detected in the majority of experiments. Clinical studies are warranted to determine if leukocyte filtration of blood will reduce the risk of transfusion transmitted viral infections.
Rawal
B D
BD
Irwin Memorial Blood Center, San Francisco, CA.
Busch
M P
MP
Endow
R
R
Garcia-de-Lomas
J
J
Perkins
H A
HA
Schwadron
R
R
Vyas
G N
GN
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology / Q000635:transmission*; D001782:Blood Donors; D002469:Cell Separation / Q000379:methods; D006678:HIV / Q000254:growth & development; D006440:Hemofiltration / Q000379:methods; D006801:Humans; D007962:Leukocytes / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology