title: Effect of plasma fibronectin on the adhesive properties of human peripheral lymphocytes.
abstract: The adhesive properties of human peripheral lymphocytes (HPL) towards immunobilized substrate in vitro have been investigated. The role of plasma fibronectin was studied in two different systems: a) immobilized fibronectin as a model of cell adhesion in the tissues; b) soluble fibronectin as a model for cell adhesion in blood circulation. Collagen (Type I) or collagen-fibronectin-coated surfaces were used as substrate for lymphocyte adhesion in system b. A very small amount of HPL (5.1 +/- 1.2%) was attached to the collagen surface. The adhesion to fibronectin or to collagen-fibronectin surfaces, however, was substantially higher, reaching a value of 34.1 +/- 3.8% and 32.7 +/- 5.8%, respectively. The addition of soluble fibronectin to system a leads to a competitive inhibition of adhesion. On the contrary, in the presence of soluble fibronectin, the augmentation of the lymphocyte adhesion to collagen was found to be dose-dependent and nearly 4 times higher as compared to the control level. This effect was found to be maximal at fibronectin concentrations of 250-300 micrograms/ml. The data demonstrate the important role of plasma fibronectin in the adhesive interactions of lymphocytes.
authors.affiliation: Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
authors.forename: G
authors.initials: G
authors.lastname: Altankov
authors.forename: L
authors.initials: L
authors.lastname: Smilenov
mesh_terms: D002448:Cell Adhesion; D003094:Collagen; D005353:Fibronectins / Q000097:blood / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D013499:Surface Properties
chemical_list: D005353:Fibronectins; D003094:Collagen