Production of monoclonal antibody to thyroglobulin from malignant thyroid carcinoma.
In this study, the production of anti-human thyroglobulin (Tg) monoclonal antibodies was attempted to detect Tg levels in serum and to study the localization of thyroid metastases in patients with thyroid-gland ablation. As a result four clones of hybridoma cell lines were obtained. By means of enzyme immunoassay (EIA) and immunohistochemical method, it was found that one of them, 2G4, produced a monoclonal antibody recognizing a malignant structural change of Tg and other three clones produced monoclonal antibodies recognizing not only the normal human Tg but also the malignant Tg. Our monoclonal antibody, 2G4, will be a hopeful reagent for the diagnosis in thyroid carcinoma.
Kusakabe
K
K
Department of Radiology, Tokyo Women's Medical College, Japan.
Mori
E
E
Kano
K
K
Ohta
Y
Y
Fujimoto
Y
Y
Mori
T
T
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002291:Carcinoma, Papillary / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D013954:Thyroglobulin / Q000276:immunology* / Q000302:isolation & purification; D013964:Thyroid Neoplasms / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D013954:Thyroglobulin