Left and right ventricular myocardial morphometry in fetal, neonatal, and adult sheep.
This study has examined left (LV) and right ventricular (RV) myocardial morphometry in perfusion-fixed hearts of late-gestation sheep fetuses, neonatal lambs, and adult sheep. During development, myocyte size, intercapillary distance, and myocyte myofibrillar and mitochondrial volume densities increased, whereas capillary density, the myocyte-to-capillary ratio, and the myocyte matrix volume density decreased. RV myocytes were larger than LV myocytes in cross section in fetuses and 4-day-old lambs. LV and RV myocytes were of similar size in 7-day-old lambs. LV and RV myocytes were of larger in older lambs and adult sheep. Differences between LV and RV myocyte volume densities of myofibrils, mitochondria, and matrix were also observed in fetuses and young lambs. As well, variation in capillary size and density was apparent between ventricles in the fetal and neonatal periods. We conclude that, in the sheep heart, 1) LV and RV morphometric differences exist during fetal and postnatal development, 2) fetal LV and RV myocardial morphometry is consistent with an RV dominance in utero, 3) rapid growth of LV and RV myocytes occurs in the perinatal period, and 4) the relative size of LV and RV myocytes does not reflect a postnatal LV dominance until between 1 and 4 wk after birth.
Smolich
J J
JJ
Centre for Early Human Development, Monash University, Clayton, Victoria, Australia.
Walker
A M
AM
Campbell
G R
GR
Adamson
T M
TM
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology / Q000502:physiology; D002196:Capillaries / Q000033:anatomy & histology; D003331:Coronary Vessels / Q000033:anatomy & histology; D005318:Fetal Heart / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D008929:Mitochondria, Heart / Q000648:ultrastructure; D009206:Myocardium / Q000166:cytology* / Q000648:ultrastructure; D012756:Sheep