title: Diazomethane derivatization of sulfamethazine: formation of isomeric products.
abstract: Reaction of 4-amino-N-(4,6-dimethyl-2-pyrimidinyl)benzenesulfonamide (sulfamethazine) with diazomethane yields not only 4-amino-N-(4,6-dimethyl-2-pyrimidinyl)-N-methylbenzenesulfonamide but also 2-(4-aminobenzenesulfonimido)-1,4,6-trimethyl-1,2-dihydro-pyrimidi ne. Yields of the latter compound are highly variable and the compound does not show a response to gas chromatography. Thus, results of gas chromatographic determinations of residues of some sulfa drugs in edible meat tissues may be erroneous when diazomethane derivatization is used.
authors.affiliation: U.S. Department of Agriculture, Biosciences Research Laboratory, Fargo, ND 58105.
authors.forename: V J
authors.initials: VJ
authors.lastname: Feil
authors.forename: G D
authors.initials: GD
authors.lastname: Paulson
authors.forename: A L
authors.initials: AL
authors.lastname: Lund
mesh_terms: D003978:Diazomethane; D007202:Indicators and Reagents; D007536:Isomerism; D013418:Sulfamethazine / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D013418:Sulfamethazine; D003978:Diazomethane