title: Immunochemical identification of desmin in Torpedo postsynaptic membranes and at the rat neuromuscular junction.
abstract: Preparations of acetylcholine receptor-rich (AChR-rich) postsynaptic membranes from electric tissue of electric rays often contain an Mr 55,000 protein (55kD protein) that has not been previously characterized. Using a monoclonal antibody (MAb 1403) against the 55kD protein from Torpedo californica and a pan-specific, anti-intermediate filament antibody (Pruss et al., 1981; Cell 27:419-428), we show that the 55kD protein has the properties expected of Torpedo desmin. By the electron microscope immunogold method applied to perfusion-fixed electric tissue, MAb 1403 labeled only cytoplasmic filaments in the electroplax. These filaments were neither more concentrated nor arranged detectably differently in postsynaptic regions relative to nonpostsynaptic regions. The 55kD protein could also be fractionated away from isolated postsynaptic membranes by gradient centrifugation. The protein is thus a minor component of the postsynaptic membrane in situ and after isolation. On semithin cryosections of rat skeletal muscle, on the other hand, MAb 1403, which recognizes rat desmin but not rat vimentin, gave strong fluorescent labeling of the postsynaptic region, weaker labeling of the Z-line, and still weaker labeling of the cell surface immediately surrounding extra-junctional nuclei. The pattern of postsynaptic labeling suggests that desmin, presumably in the form of intermediate filaments, occurs near the AChR-rich crests of the junctional folds, but is particularly concentrated among and around the ends of the folds. Similar results were obtained with a second monoclonal antibody raised against authentic desmin. These results suggest that desmin intermediate filaments may have an important role in organization of the postsynaptic cytoplasm in rat muscle.
authors.affiliation: Department of Physiology, University of North Carolina, Chapel Hill 27599.
authors.forename: R
authors.initials: R
authors.lastname: Sealock
authors.forename: A A
authors.initials: AA
authors.lastname: Murnane
authors.forename: D
authors.initials: D
authors.lastname: Paulin
authors.forename: S C
authors.initials: SC
authors.lastname: Froehner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003893:Desmin / Q000032:analysis*; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000032:analysis / Q000648:ultrastructure; D008854:Microscopy, Electron; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000032:analysis* / Q000648:ultrastructure; D009469:Neuromuscular Junction / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D014101:Torpedo / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D003893:Desmin