Fractionation of Sumatran pit viper (Trimeresurus sumatranus sumatranus) venom by DEAE-Sephacel ion exchange chromatography and some biological properties of the fractions.
Sumatran pit viper (Trimeresurus sumatranus sumatranus) venom was fractionated by DEAE-Sephacel ion exchange chromatography into seven fractions. Fractions 4, 5 and 6 were lethal to mice and exhibited strong hemorrhagic activity, as well as some enzymatic activities. Fraction 6 also exhibited potent anticoagulant and thrombin-like activities. Analysis of the biological and enzymatic properties of the three lethal fractions suggests that the major lethal component of fractions 4 and 5 may be the hemorrhagic principle, and that the lethality of fraction 6 may be due to the hemorrhagic principle and/or the anticoagulant principle.
Tan
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NH
Department of Biochemistry, University of Malaya, Kuala Lumpur, Malaysia.
Tan
C S
CS
D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000302:isolation & purification* / Q000633:toxicity; D004798:Enzymes / Q000032:analysis*; D007928:Lethal Dose 50; D013056:Spectrophotometry, Ultraviolet
D003435:Crotalid Venoms; D004798:Enzymes