Viability and functional integrity of washed platelets.
The viability and functional integrity of saline- and ACD-saline-washed platelets were compared with those of unwashed platelets. After template bleeding time (TBT) was measured, 15 healthy volunteers underwent plateletpheresis and ingested 600 mg of aspirin. Autologous 111In-labeled platelets were transfused: unwashed (n = 5), washed with 0.9 percent saline solution (SS) (n = 5), and washed with a buffered 12.6 percent solution of ACD-A in 0.9 percent saline solution (n = 5). After transfusion, we measured TBT at 1, 4, and 24 hours; platelet survival at 10 minutes and 1, 4, and 24 hours and daily for 6 days; and the percentage of uptake in liver and spleen by quantitative whole-body radionuclide scintigraphy at 24 and 190 hours. We found that saline washing affected platelet recovery, 23.47 +/- 12 percent (p less than 0.001) as compared to 52.43 +/- 17 percent (p less than 0.002) for ACD-saline and 73.17 +/- 8 percent for control; that saline washing resulted in a greater liver uptake than control and ACD-saline-washed platelets (31.9 +/- 8% [p less than 0.001] vs 17.7 +/- 4.1 and 19.3 +/- 2.1% [p greater than 0.1], respectively); that, unlike control and ACD-saline-washed platelets, saline-washed platelets did not shorten bleeding time; and that neither type of washing affected survival. Although ACD-saline washing affects recovery, it also results in intact function, normal survival, higher recovery than SS platelets, and no significant liver uptake.
Pineda
A A
AA
Blood Bank Service, Mayo Clinic, Rochester, Minnesota.
Zylstra
V W
VW
Clare
D E
DE
Dewanjee
M K
MK
Forstrom
L A
LA
D001760:Bleeding Time; D001792:Blood Platelets / Q000502:physiology; D002021:Buffers; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D019343:Citric Acid; D005947:Glucose / Q000031:analogs & derivatives; D006801:Humans; D007205:Indium Radioisotopes; D008099:Liver / Q000378:metabolism; D010976:Platelet Count; D017713:Platelet Transfusion; D012965:Sodium Chloride; D013154:Spleen / Q000378:metabolism
D002021:Buffers; D007205:Indium Radioisotopes; C002113:acid citrate dextrose; D019343:Citric Acid; D012965:Sodium Chloride; D005947:Glucose