A jack bean protein similar to pea seed ferritin and unrelated to concanavalin A is the only iron storage protein in jack bean seeds, although concanavalin A can form ferritin-like iron cores in vitro.
Iron storage in living organisms is performed by ferritins. These proteins are built up from 24 subunits organized in a spherical shell forming a coat to a core of bioavailable iron. Recently, it was observed that concanavalin A from jack bean can form in vitro iron cores similar to those of animal ferritins (Yariv, J., Kalb, A. J., Helliwell, J. R., Papiz, M. Z., Bauminger, E. R., and Nowik, I. (1988) J. Biol. Chem. 263, 13508-13510). From this observation and from the comparison of the three-dimensional structures of horse spleen ferritin and of the form of concanavalin A which forms soluble polynuclear iron complexes, these authors suggested that concanavalin A is the apoferritin of jack bean seeds. On the basis of immunological and biochemical results, we report here that a protein purified from jack bean seeds, unrelated to concanavalin A and similar to plant seed ferritins, is responsible for iron storage in jack bean seeds. Furthermore, concanavalin A does not contain iron in vivo. Therefore we conclude that a unique protein, ferritin, stores iron in vivo in jack bean seeds and that concanavalin A provides an unusual model for studying the formation of iron cores inside a protein shell.
Briat
J F
JF
Centre National de la Recherche Scientifique URA 57, Université Joseph Fourier, Grenoble, France.
Massenet
O
O
Laulhère
J P
JP
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003208:Concanavalin A / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000378:metabolism*; D005293:Ferritins / Q000378:metabolism*; D006736:Horses; D007501:Iron / Q000378:metabolism*; D037121:Plant Lectins; D010946:Plants, Medicinal; D013154:Spleen / Q000378:metabolism
D000596:Amino Acids; D037121:Plant Lectins; D003208:Concanavalin A; D005293:Ferritins; D007501:Iron