title: Cloning of a hemolysin gene from Leptospira interrogans serovar hardjo.
abstract: A DNA fragment encoding both hemolysin and sphingomyelinase C activity was cloned from the pathogenic bacterium Leptospira interrogans serovar hardjo. Initial clones were obtained by screening a genomic library in EMBL3 for hemolytic activity. Both hemolytic and sphingomyelinase C activities were coded for by a 3.9-kilobase BamHI fragment. The hemolysin was expressed from its own promoter in Escherichia coli K-12. Similar DNA sequences were also present in the serovars tarassovi and ballum.
authors.affiliation: Department of Bacteriology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
authors.forename: G
authors.initials: G
authors.lastname: del Real
authors.forename: R P
authors.initials: RP
authors.lastname: Segers
authors.forename: B A
authors.initials: BA
authors.lastname: van der Zeijst
authors.forename: W
authors.initials: W
authors.lastname: Gaastra
mesh_terms: D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification; D005796:Genes; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D007921:Leptospira interrogans / Q000235:genetics*; D014774:Virulence
chemical_list: D004269:DNA, Bacterial; D006460:Hemolysin Proteins