Immuno-affinity purification of specific antibodies against vasoactive intestinal polypeptide (VIP) on VIP (1-10)-linked polydimethylacrylamide resin.
A novel method for immuno-affinity purification of specific antibodies against amino-terminal (N-terminal) porcine vasoactive intestinal polypeptide (p-VIP) was developed. The antiserum VP (No. 6203) elicited by p-VIP-immunoglobulin G (IgG) conjugate was heterogenous and reacted not only with the N-terminal fragments of p-VIP but also with carboxy-terminal (C-terminal) fragments. To obtain specific antibodies against the N-terminal fragment of p-VIP, antiserum VP was purified by column chromatography on N-terminal decapeptide (p-VIP 1-10)-linked polydimethylacrylamide resin. The antibody thus obtained was highly specific to the N-terminal sequence of p-VIP and hardly reacted with the C-terminal fragments of VIP in enzyme-linked immunosorbent assay.
Takeyama
M
M
Hatano
M
M
Otaka
A
A
Fujii
N
N
Yajima
H
H
D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D007120:Immunochemistry; D012116:Resins, Plant; D014660:Vasoactive Intestinal Peptide / Q000276:immunology*
D000906:Antibodies; D012116:Resins, Plant; D014660:Vasoactive Intestinal Peptide