Diazomethane derivatization of sulfamethazine: formation of isomeric products.
Reaction of 4-amino-N-(4,6-dimethyl-2-pyrimidinyl)benzenesulfonamide (sulfamethazine) with diazomethane yields not only 4-amino-N-(4,6-dimethyl-2-pyrimidinyl)-N-methylbenzenesulfonamide but also 2-(4-aminobenzenesulfonimido)-1,4,6-trimethyl-1,2-dihydro-pyrimidi ne. Yields of the latter compound are highly variable and the compound does not show a response to gas chromatography. Thus, results of gas chromatographic determinations of residues of some sulfa drugs in edible meat tissues may be erroneous when diazomethane derivatization is used.
Feil
V J
VJ
U.S. Department of Agriculture, Biosciences Research Laboratory, Fargo, ND 58105.
Paulson
G D
GD
Lund
A L
AL
D003978:Diazomethane; D007202:Indicators and Reagents; D007536:Isomerism; D013418:Sulfamethazine / Q000032:analysis*
D007202:Indicators and Reagents; D013418:Sulfamethazine; D003978:Diazomethane