A modified anti-roll plate as a remedy for the ill-effects of electrical charge during cryosectioning.
This report describes problems commonly encountered in cryosectioning of fragile tissues, such as jamming and scrambling of sections between knife and anti-roll plate. The electrostatic nature of the factors causing these derangements is demonstrated here using simple methods, and several modifications to the anti-roll plate surface are tested for their help in obviating these difficulties. The anti-roll plate material found to be most effective consists of a metal oxide-coated glass which is commercially available at low cost.
Viebahn
C
C
Institute of Anatomy, University of Bonn, Federal Republic of Germany.
Lüttenberg
H P
HP
D000818:Animals; D006224:Cricetinae; D004560:Electricity; D004622:Embryo, Mammalian / Q000033:anatomy & histology; D005629:Frozen Sections; D005898:Glass; D051379:Mice; D008867:Microtomy; D011817:Rabbits; D051381:Rats