Liquid chromatographic determination of miconazole nitrate in creams and suppositories.
A rapid method has been developed for the determination of miconazole nitrate in creams and suppositories. The sample is dissolved in ethanol, diluted in acetonitrile-water (1 + 1), and injected onto a C18 column. The mobile phase consists of 55% acetonitrile, a triethylammonium phosphate buffer, and an ion-pairing agent. The total run time is less than 4 min, and the active ingredient is determined using absorbance detection at 214 nm. The mean recovery of miconazole from spiked placebo samples was 99.7 +/- 0.7% for the cream samples at the 2% level and 98.8 +/- 0.3% for the suppository samples at the 4% level.
Tyler
T A
TA
Herbert V. Shuster, Inc., Quincy, MA 02171.
Genzale
J A
JA
D002849:Chromatography, Gas; D003358:Cosmetics / Q000032:analysis*; D005410:Flame Ionization; D008825:Miconazole / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013488:Suppositories / Q000032:analysis*
D003358:Cosmetics; D013488:Suppositories; D008825:Miconazole