Biodegradable microspheres: vaccine delivery system for oral immunization.
The potential of biocompatible and biodegradable microspheres as a controlled release oral vaccine delivery system has been examined. Orally-administered 1-10 micron microspheres composed of poly (DL-lactide-co-glycolide) were specifically taken up into the Peyer's patch lymphoid tissue of the gut, where those greater than or equal to 5 micron remained for up to 35 days. Microspheres less than 5 micron disseminated within macrophages to the mesenteric lymph nodes and spleen. In contrast to soluble staphylococcal enterotoxin B toxoid, oral immunization with enterotoxoid in microspheres induced circulating toxin-specific antibodies and a concurrent secretory IgA anti-toxin response in saliva and gut fluid.
Eldridge
J H
JH
Gilley
R M
RM
Staas
J K
JK
Moldoveanu
Z
Z
Meulbroek
J A
JA
Tice
T R
TR
D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation; D001672:Biocompatible Materials; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010581:Peyer's Patches / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage*
D001672:Biocompatible Materials; D014612:Vaccines