title: Radiolabeled, nonspecific, polyclonal human immunoglobulin in the detection of focal inflammation by scintigraphy: comparison with gallium-67 citrate and technetium-99m-labeled albumin.
abstract: The accumulation of nonspecific polyclonal human immunoglobulin (IgG) radiolabeled with 125I or 111In was compared to that of [67Ga]citrate and [99mTc]albumin in rats with deep thigh inflammation due to Escherichia coli infection. Serial scintigrams were acquired at 1, 3, 24, and in some cases, 48 hr after injection. As early as 3 hr postinjection, [111In]IgG showed greater accumulation at the lesion than [99mTc]HSA (p less than 0.01). Both [125I]IgG and [111In]IgG showed greater accumulation than [67Ga]citrate (p less than 0.01). At 24 hr, IgG image definition increased, while HSA image definition decreased, and the intensity of accumulation of both IgG preparations was greater than that of [67Ga]citrate or [99mTc]HSA (p less than 0.01). At all imaging times, [67Ga]citrate accumulation was surprisingly low. In inflammation produced by Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, Candida albicans, or turpentine, [111In]IgG accumulation was similar to the results obtained with Escherichia coli. These studies suggest that focal sites of inflammation can be detected with radiolabeled nonspecific human polyclonal IgG.
authors.affiliation: Infectious Disease Unit of the Medical Service, Massachusetts General Hospital, Boston 02114.
authors.forename: R H
authors.initials: RH
authors.lastname: Rubin
authors.forename: A J
authors.initials: AJ
authors.lastname: Fischman
authors.forename: M
authors.initials: M
authors.lastname: Needleman
authors.forename: R
authors.initials: R
authors.lastname: Wilkinson
authors.forename: R J
authors.initials: RJ
authors.lastname: Callahan
authors.forename: B A
authors.initials: BA
authors.lastname: Khaw
authors.forename: W P
authors.initials: WP
authors.lastname: Hansen
authors.forename: P B
authors.initials: PB
authors.lastname: Kramer
authors.forename: H W
authors.initials: HW
authors.lastname: Strauss
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000150:complications / Q000000981:diagnostic imaging; D002177:Candidiasis / Q000150:complications / Q000000981:diagnostic imaging; D002951:Citrates; D019343:Citric Acid; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007205:Indium Radioisotopes; D007249:Inflammation / Q000000981:diagnostic imaging* / Q000209:etiology; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008297:Male; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D013668:Technetium Tc 99m Aggregated Albumin; D014425:Turpentine
chemical_list: D002951:Citrates; D007074:Immunoglobulin G; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D013668:Technetium Tc 99m Aggregated Albumin; D019343:Citric Acid; D014425:Turpentine