Plasma clearance of bile acids in the rat: hepatic uptake under physiological conditions without countertransport.
Studies in rats by others indicated that sulfobromophthalein (BSP), bilirubin and indocyanine green are taken up by the liver and can be transported back to plasma against the prevailing concentration gradient (= countertransport). The present in vivo study was designed to determine whether the bile acids cholic acid and taurocholic acid under physiological conditions undergo appreciable countertransport as has been suggested by experiments in isolated hepatocytes. Experiments with BSP (controls) showed that injections of unlabeled BSP into rats five minutes after the administration of radiolabeled BSP was followed by a release of radioactivity into plasma (BSP-countertransport). In contrast bile acid countertransport could not be demonstrated, no matter whether it was tested 1, 5 or 8 minutes after the administration of radiolabeled cholic- or taurocholic acid.
Gärtner
U
U
Department of Medicine, University of Heidelberg, F.R.G.
Gundert-Remy
U
U
Stiehl
A
A
Schulz
T
T
Götz
R
R
Walter
B
B
Kommerell
B
B
D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood*; D001693:Biological Transport, Active; D002463:Cell Membrane Permeability; D019826:Cholic Acid; D002793:Cholic Acids / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate; D051381:Rats; D011919:Rats, Inbred Strains; D013656:Taurocholic Acid / Q000097:blood
D001647:Bile Acids and Salts; D002793:Cholic Acids; D013656:Taurocholic Acid; D019826:Cholic Acid