Ovarian early postovulatory development and oestrogen production in rainbow trout (Salmo gairdneri R.) from a spring-spawning strain.
Ovarian early postovulatory development and oestrogen production were studied in rainbow trout from a spring-spawning strain, 5 to 50 days postovulation (PO). Early postovulatory development was assessed by the ovarian development index, corresponding to the relative volume occupied in the ovary by oocytes in an advanced stage of differentiation (containing cortical alveoli). This index was shown to be related to the maximal oocyte diameter and to the time since ovulation, as well as to metabolic criteria. In contrast, gonadosomatic index was not correlated with any of these, confirming its unreliability for assessing the gonadal development, at least at this stage. Size of follicles and presence of vitellus (lipoprotein) in the oocytes of several animals indicate that ovarian development was more advanced in our females than in other studies. It is suggested that young follicles start developing before ovulation of the preceding batch of oocytes. Oestradiol (E2) plasma levels were low but detectable, and showed, in some females, peak values (max 5.5 ng/ml) within the first month PO. Besides, in vitro metabolism was studied using two isotopes of androstenedione as precursors. No sharp discrepancies were observed between the two types of incubation. Conjugation of the precursor or its metabolites was high but decreased with time postovulation. This suggests that it proceeds from postovulatory follicles. E2 production potential was demonstrated from the shortest time postovulation. Moreover, it increased with ovarian development, suggesting that E2 is synthesized in developing follicles.
de Mones
A
A
Institut National de la Recherche Agronomique, Laboratoire de Physiologie des Poissons, Campus de Beaulieu, Rennes, France.
Fostier
A
A
Cauty
C
C
Jalabert
B
B
D000735:Androstenedione / Q000097:blood; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D004958:Estradiol / Q000097:blood; D004967:Estrogens / Q000096:biosynthesis*; D005260:Female; D009865:Oocytes / Q000166:cytology; D010053:Ovary / Q000254:growth & development* / Q000502:physiology; D010060:Ovulation; D012487:Salmonidae / Q000502:physiology*; D012741:Sexual Maturation; D014337:Trout / Q000502:physiology*
D004967:Estrogens; D000735:Androstenedione; D004958:Estradiol; D001141:Aromatase