[Localization and invasion of the femur in the rat by a soft tissue tumor induced by pellets made of hydroxypropylcellulose mixed with 20-methylcholanthrene: special reference to effects of the periosteum].
Pellets made of a carcinogenic substance, methylcholanthrene admixed with a high molecular substance, hydroxypropycellulose were implanted into the thigh of 90 Wistar white female rats of 5 weeks of age (3 groups, 30 rats each). Ten control rats did not receive the pellets. In group 1, the pellets were placed at the window made at the cortex of the right femur; in group 2, into the space between the cortex and the periosteum; in group 3, into the pouch created outside of the periosteum. Twenty weeks after implantation, spindle cell sarcoma developed in 28 rats of group 1, 27 of group 2, and 21 of group 3. Tumor invasions into the bone marrow of the femur were seen in 14 rats in group 1 (46.7%), 5 in group 2 (18.5%). In group 3, however, tumor invasion was not observed. There were significant differences in the frequency of tumor invasion between group 1 and 2 (p less than 0.01) as well as between group 2 and 3 (p less than 0.05). At implant sites, group 2 showed thinning out of periosteum, while group 3 exhibited thickening of both cortex and periosteum. This experimental results suggested that periosteum provides an important barrier in the bone against tumor invasion.
Yoshida
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Department of Orthopaedic Surgery, Yokohama City University School of Medicine Kanagawa, Japan.
D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives*; D005260:Female; D005266:Femoral Neoplasms / Q000139:chemically induced / Q000473:pathology*; D008748:Methylcholanthrene; D009361:Neoplasm Invasiveness; D010521:Periosteum / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000139:chemically induced / Q000473:pathology*
D008748:Methylcholanthrene; D002482:Cellulose; C008079:hydroxypropylcellulose