Analysis of a soluble lipid-protein complex carrying endogenous 11-cis retinaldehyde from bovine retinal pigment epithelium.
A soluble lipid-protein complex in bovine retinal pigment epithelium is shown to carry endogenous 11-cis retinaldehyde, in the extent of 15% of the total 11-cis retinaldehyde found in this tissue. The complex, analyzed with respect to its chemical composition, exhibits a lipid composition close resembling the lipid composition of the rod outer segment membrane; the SDS-PAGE evidences the presence of a number of protein bands, two of which of 34 and 27 kDa appear glycoproteins. Finally, the lipid-protein complex exhibits a discrete level of a Cathepsin D-like protease activity. From the above, the possibility is discussed that the soluble lipid-protein complex could represent some phagolysosomal inclusion occurring in the pigment epithelial cells upon rod outer segment phagocytosis.
Livrea
M A
MA
Institute of Biological Chemistry, University of Palermo, Italy.
Bongiorno
A
A
Bono
A
A
Tesoriere
L
L
Gueli
M C
MC
Nicotra
C
C
Pandolfo
L
L
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002784:Cholesterol / Q000032:analysis*; D002850:Chromatography, Gel; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008074:Lipoproteins / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D010743:Phospholipids / Q000032:analysis*; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D012172:Retinaldehyde / Q000378:metabolism*; D012176:Retinoids / Q000378:metabolism*; D014280:Triglycerides / Q000032:analysis
C020932:11-cis-retinal-binding protein; D002352:Carrier Proteins; D008074:Lipoproteins; D010743:Phospholipids; D012176:Retinoids; D014280:Triglycerides; D002784:Cholesterol; D012172:Retinaldehyde