Cellular origin and distribution of transforming growth factor-beta in the normal rat myocardium.
Transforming growth factor-beta (TGF-beta) is a biologically active polypeptide present in normal tissues as well as transformed cells. Two structurally related forms of this peptide are TGF-beta 1 and TGF-beta 2. Using freshly isolated cardiomyocytes and non-myocyte heart cells, and a [32P]- labelled cDNA probe to human TGF-beta 1, we demonstrated that mRNA for TGF-beta 1 could be detected only in the nonmyocyte fraction of heart cells. In the present study, the distribution of TGF-beta 1 in the heart was determined by immunofluorescence staining by use of a polyclonal antibody to porcine TGF-beta 1 in cryostat sections of rat heart. Immunofluorescence staining was intense around the blood vessels and radially diffuse in the surrounding myocardium.
Eghbali
M
M
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York.
D000818:Animals; D002469:Cell Separation; D007150:Immunohistochemistry; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D015291:Transforming Growth Factors / Q000378:metabolism*
D012333:RNA, Messenger; D015291:Transforming Growth Factors