Isolation of modified histone H3 from ultraviolet-irradiated deoxyribonucleoprotein by reversed-phase high-performance liquid chromatography.
A rapid reversed-phase high-performance liquid chromatography procedure for the isolation of histone H3 and/or of thymine modified at the lysine residue histone H3 from uv-irradiated deoxyribonucleoprotein and DNA-protein complex is reported. The system utilizes a C8 Ultrasphere macroporous column and an acetonitrile "inverse or negative gradient."
Kurochkina
L P
LP
A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Kolomijtseva GYa
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003852:Deoxyribonucleoproteins / Q000528:radiation effects; D004586:Electrophoresis; D006657:Histones / Q000302:isolation & purification*; D014466:Ultraviolet Rays
D003852:Deoxyribonucleoproteins; D006657:Histones