Bioluminescent flow sensors: L-lactate dehydrogenase activity determination in serum.
The catalytic activity of serum L-lactate dehydrogenase (LDH), was determined by monitoring the NADH produced by LDH with bacterial bioluminescent enzymes immobilized on a nylon coil. The LDH reaction of L-lactate with NAD took place in a flow-through mixing coil that preceded the bioluminescent detector coil. The response was linear from 1 to 5000 U/l at 37 degrees C and from 3 to 2000 U/l at 25 degrees C. The intra- and inter-assay reproducibility (CV%) were less than 10% and recovery range was 92% to 110%. The results agreed well with those obtained with a spectrophotometric method.
Girotti
S
S
Institute of Chemical Sciences, University of Bologna, Italy.
Bassoli
C
C
Cascione
M L
ML
Ghini
S
S
Carrea
G
G
Bovara
R
R
Roda
A
A
Motta
R
R
Petilino
R
R
D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008163:Luminescent Measurements; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction
D004800:Enzymes, Immobilized; D009243:NAD; D007770:L-Lactate Dehydrogenase