Different handling of antigen by macrophages of low-responder C57BL/10ScSn strain and high-responder A/J strain of mice. II. Antigen uptake.
The uptake of 51Cr-SRBC and 125I-ARS-BG6 in the liver and spleen of non-immune low IgG responder C57BL/10ScSn mice is higher than in the high-responder A/J strain. After immunization with SRBC, the uptake in the spleen of A/J mice is ten times higher and that in the liver three times higher than in C57BL/10ScSn mice. The higher uptake of the well responding A/J strain, which is antigen specific, is due not only to a higher level of opsonizing antibodies. Endotoxin stimulation, which increases the IgG formation of the low-responding strain, does not increase the uptake in this strain. In the low-responder strain there is a considerable level of free 51Cr in the serum and kidney within 5 min after antigen injection, suggesting a high catabolic rate of the antigen. In contrast, at that time in the A/J strain virtually all of the radioactivity is still bound to the erythrocyte fraction. Ultrastructural observations suggest that in the low-responding strain the antigen residues survived in macrophages for a long period of time. The results provide evidence of differences between the metabolic activity of macrophage populations of both strains which could be related to the low IgG response of the strain C57BL/10ScSn.
Ríhová
B
B
Institute of Microbiology, Czechoslovak Academy of Sciences, Praha.
Vĕtvicka
V
V
Rossmann
P
P
Plocová
D
D
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002860:Chromium Radioisotopes; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007114:Immunization; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D013045:Species Specificity
D000941:Antigens; D002860:Chromium Radioisotopes