title: IR spectral changes of bovine serum albumin upon surface adsorption.
abstract: The adsorption of bovine serum albumin from flowing solutions onto germanium and three polyetherurethanes varying in soft segment content was studied by a Fourier transform infrared/attenuated total reflectance technique. Spectral differences observed in the amide I, II, and III regions upon adsorption to all four surfaces were consistent with a loss of helix and gain of beta-structure. There appeared to be a slight difference between BSA adsorbed to germanium and the PEUs, but no distinction could be made between BSA adsorbed to the different PEUs.
authors.affiliation: Department of Chemical Engineering, University of Washington, Seattle 98195.
authors.forename: T J
authors.initials: TJ
authors.lastname: Lenk
authors.forename: B D
authors.initials: BD
authors.lastname: Ratner
authors.forename: R M
authors.initials: RM
authors.lastname: Gendreau
authors.forename: K K
authors.initials: KK
authors.lastname: Chittur
mesh_terms: D000327:Adsorption; D001704:Biopolymers; D046911:Macromolecular Substances; D012710:Serum Albumin, Bovine; D013055:Spectrophotometry, Infrared
chemical_list: D001704:Biopolymers; D046911:Macromolecular Substances; D012710:Serum Albumin, Bovine