title: Hairpin extension. A general method for the improvement of sensitivity of oligonucleotide probes.
abstract: A general and sensitive detection method of target DNA is described. The system is based on an oligonucleotide probe labeled to high specific activity. This involves a novel oligonucleotide design incorporating at the 3' end a hairpin structure, allowing extension by polymerase reaction.
authors.affiliation: Menzies School of Health Research, Darwin, N.T., Australia.
authors.forename: K S
authors.initials: KS
authors.lastname: Sriprakash
authors.forename: J
authors.initials: J
authors.lastname: Hartas
mesh_terms: D002689:Chlamydia / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D010957:Plasmids
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D009841:Oligonucleotides