Human skin fibroblast in vitro tetraploidy. Flow cytometric DNA assay used to confirm metaphase assay in patients with various colonic diseases.
One hundred twenty-two patients with various colon pathologies (43 colorectal carcinoma patients exclusive of the known autosomal dominant colorectal cancer syndromes, 31 patients with solitary colorectal adenomas, 27 patients with ulcerative colitis, and 21 controls with no evidence of colorectal cancer) were investigated for in vitro tetraploidy in dermal fibroblasts cultures using a metaphase assay for determination of numerical chromosomal alterations. Later, stationary cultures of these skin fibroblasts were investigated with DNA flow cytometry. There was good correlation between the percentage of tetraploid metaphase cells and the percentage of nuclei with a flow cytometric DNA index of 2. Using a linear discrimination function to classify the flow cytometric data, the peak with DNA index of 2 was the most important parameter, supplemented by the region with DNA index greater than 2, whereas the region with DNA index between 1 and 2 probably represented a different subpopulation. We have thus demonstrated that only the region with a DNA index of 2 or greater is suitable in determining in vitro tetraploidy in stationary skin fibroblast cultures.
Svendsen
L B
LB
Department of Surgical Gastroenterology F, Bispebjerg Hospital, Copenhagen, Denmark.
Larsen
J K
JK
Christensen
I J
IJ
D003093:Colitis, Ulcerative / Q000235:genetics / Q000473:pathology; D003108:Colonic Diseases / Q000235:genetics* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005347:Fibroblasts / Q000473:pathology / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D008677:Metaphase; D011003:Ploidies; D012867:Skin / Q000473:pathology / Q000648:ultrastructure
D004273:DNA, Neoplasm; D004247:DNA