title: Preparation of plant DNA for separation by pulsed field gel electrophoresis.
abstract: A method was developed for the preparation of completely intact plant DNA embedded in agarose, and suitable for restriction enzyme digestion. Digestion with restriction enzyme was carried out according to modified protocols of Anand and Kenwrick et al. The new method of DNA isolation allows the separation of high molecular weight plant DNA by pulsed field gel electrophoresis.
authors.affiliation: Laboratory of Physiological Chemistry, University of Gent, Belgium.
authors.forename: K M
authors.initials: KM
authors.lastname: Devos
authors.forename: D
authors.initials: D
authors.lastname: Vercruysse-Dewitte
mesh_terms: D015139:Blotting, Southern; D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods; D008970:Molecular Weight; D010944:Plants / Q000235:genetics*; D011523:Protoplasts / Q000032:analysis*; D013048:Specimen Handling; D014908:Triticum / Q000235:genetics
chemical_list: D004247:DNA