Genetic analysis of the lytic replicon of bacteriophage P1. I. Isolation and partial characterization.
Despite the extensive genetic analysis of bacteriophage P1, the region of the viral genome that is responsible for its lytic (vegetative) replication has not been identified. In this paper we describe the identification of various fragments of P1 DNA that can replicate an otherwise replication-defective lambda vector when they are cloned into that vector. The fragments share a 2800 base-pair segment of the P1 genome that is located adjacent to the immI region of the phage. Replication mediated by the cloned P1 fragments is abolished by the product of the P1 c1 gene, the repressor of phage lytic functions. Since these properties resemble those of the P1 lytic replicon, we suggest that the 2800 base-pair segment identified here contains that replicon.
Cohen
G
G
Central Research and Development Department, E. I. du Pont de Nemours and Co., Wilmington, DE 19898.
Sternberg
N
N
D001482:Base Composition; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D008957:Models, Genetic; D010957:Plasmids; D012093:Replicon; D015183:Restriction Mapping; D014779:Virus Replication
D004279:DNA, Viral