[Atherogenic modifications of low density lipoproteins during fibrin formation and fibrinolysis].
Low density lipoproteins (LDL) were modified after incubation with fibrinogen and fibronectin at physiological concentrations in presence of thrombin and, following the fibrin formation, in presence of plasmin. The modified LDL (LDL-F) isolated from plasmin digested fibrin by means of gel permeation chromatography on Sepharose 6B, were associated with fibrinogen and fibronectin degradation products. The LDL-F differed from control LDL in their physico-chemical properties: LDL-F contained the degraded apoprotein B, its electrophoretic mobility was increased, cholesterol/protein ratio as well as flotation coefficient at d = 1.063 were decreased. The effect of LDL-F on lipid accumulation was studied. Content of cholesterol esters in macrophages incubated with LDL-F was higher 3.8-fold as compared with that of the cells incubated with control LDL. Thus, after incubation of LDL with fibrinogen and thrombin, 20% of the lipoprotein was bound to fibrin. The data obtained suggest that thrombosis may promote both LDL deposition in the vascular intercellular matrix and cellular lipid accumulation.
Chulkova
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TM
D001161:Arteriosclerosis / Q000097:blood*; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D005337:Fibrin / Q000096:biosynthesis*; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005342:Fibrinolysis; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008264:Macrophages / Q000032:analysis
D005338:Fibrin Fibrinogen Degradation Products; D008077:Lipoproteins, LDL; D005337:Fibrin; D002784:Cholesterol
Aterogennye modifikatsii lipoproteidov nizkoĭ plotnosti v protsesse obrazovaniia fibrina i fibrinoliza.