title: Synthesis of frog virus 3 proteins occurs on intermediate filament-bound polyribosomes.
abstract: Immunogold labeling and biochemical methods were used to localize the site of viral protein synthesis in frog virus 3 (FV3)-infected baby hamster kidney (BHK) cells. Immunogold labeling studies of Triton-extracted (cytoskeletons), FV3-infected BHK cells with antivimentin antibodies showed that the major components of the detergent-resistant residue are the intermediate filaments (IF) and polyribosomes. Double immunogold labeling studies with anti-FV3 and antivimentin antibodies revealed that FV3 proteins are associated with polyribosomes that are bound to the IF network. Biochemical studies of the cytoskeletons prepared from FV3-infected cells showed that a substantial fraction of all newly synthesized FV3 proteins associate with the cytoskeleton and that the association is not disrupted by colchicine (microtubule-disrupting drug) or cytochalasin B (microfilament-disrupting drug). Together the above studies suggest that IF may provide the scaffold for the attachment of polyribosomes that are active in viral protein synthesis.
authors.affiliation: Department of Virology & Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 38105.
authors.forename: K
authors.initials: K
authors.lastname: Murti
authors.forename: R
authors.initials: R
authors.lastname: Goorha
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003599:Cytoskeleton / Q000378:metabolism*; D004267:DNA Viruses / Q000378:metabolism*; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D014746:Vimentin; D014764:Viral Proteins