Micro-ELISA for the quantitation of human urinary and serum retinol-binding protein.
A highly sensitive enzyme linked immunoadsorbent assay for determining retinol-binding protein in urine and serum is described. Commercially available reagents are used. The standard curve ranges from 1.2-24 micrograms/l, recovery of retinol-binding protein added to urine was 99-102% (n = 10) and dilution of urine was linear. The within-assay coefficient of variation ranged from 1.2-3.1% and the day-to-day coefficient of variation from 9.2-10.5% depending on concentration. The correlation with urinary retinol-binding protein determined by radioimmunoassay was good (n = 90, r = 0.95). In vitro experiments show that retinol-binding protein is stable in urine with pH 5.2.
Jensen
T
T
Steno Memorial Hospital, Gentofte, Denmark.
Deckert
M
M
Dawnay
A
A
Feldt-Rasmussen
B
B
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008832:Microchemistry; D011863:Radioimmunoassay / Q000379:methods; D012177:Retinol-Binding Proteins / Q000032:analysis* / Q000652:urine
D012177:Retinol-Binding Proteins