Two peptides related to platelet-derived growth factor are present in human wound fluid.
Platelet-derived growth factor (PDGF) has been suggested to play an important role in wound healing. In this study wound fluid collected from patients after radical mastectomy was analyzed for PDGF-related factors. Analysis revealed the presence of two PDGF-related peptides of 16-17 kDa and 34-36 kDa. These PDGF-related peptides were purified by using anti-PDGF immunoaffinity techniques and found to possess both chemotactic and mitogenic activities for NIH 3T3 cells in vitro. Surprisingly, we could not detect authentic native PDGF (30 kDa) or PDGF A or B chain in any of the wound-fluid samples examined. These results indicate that PDGF-related peptides are present in normal human wound fluid, whereas PDGF A- and B-chain peptides are absent or only present in trace amounts relative to the total PDGF-related biological activity.
Matsuoka
J
J
Department of Biology, University of South Florida, Tampa 33620.
Grotendorst
G R
GR
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D046911:Macromolecular Substances; D015409:Mastectomy, Radical; D051379:Mice; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000031:analogs & derivatives* / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D014947:Wounds and Injuries / Q000503:physiopathology*
D046911:Macromolecular Substances; D010982:Platelet-Derived Growth Factor