title: Biochemical examination on the increased superoxide dismutase-like plasma substance observed in a state of acute gastric mucosal lesions.
abstract: When experimental acute gastric mucosal lesions were produced in guinea pig by water-immersion and restraint stress, superoxide dismutase (SOD)-like substance in the plasma increased. On analysis by gel filtration, it was shown that the molecular weight of the increased SOD-like plasma substance was about 130,000, and even after treatment with trypsin, 84% of this substance remained. Since the molecular weight of intracellular SOD is about 40,000, it seems that this substance is similar to extracellular SOD, located on the endothelial cell-surface, as previously reported by Marklund et al. Our results suggest that in the presence of acute gastric mucosal lesions, SOD-like plasma substance is not identical to intracellular SOD, which derived from cell destruction by stress or free radical-induced microvascular damage or by hemolysis. Furthermore, this substance may itself work as a scavenger of free radicals generated under conditions, such as these described in the present experiment.
authors.affiliation: Third Department of Internal Medicine, School of Medicine, Tohoku University, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohara
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: D
authors.initials: D
authors.lastname: Shibuya
authors.forename: Y
authors.initials: Y
authors.lastname: Eda
authors.forename: T
authors.initials: T
authors.lastname: Yuki
authors.forename: S
authors.initials: S
authors.lastname: Asaki
authors.forename: T
authors.initials: T
authors.lastname: Toyota
authors.forename: T
authors.initials: T
authors.lastname: Kurokawa
authors.forename: R
authors.initials: R
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D005753:Gastric Mucosa; D006168:Guinea Pigs; D007101:Immersion; D008297:Male; D008970:Molecular Weight; D012149:Restraint, Physical; D013276:Stomach Ulcer / Q000201:enzymology* / Q000209:etiology; D013312:Stress, Physiological / Q000150:complications / Q000201:enzymology; D013482:Superoxide Dismutase / Q000097:blood*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D013482:Superoxide Dismutase; D014357:Trypsin