title: Perinatal lethality (ple): a mutation caused by integration of a transgene into distal mouse chromosome 15.
abstract: We have used cytogenetic and recombinational analysis to determine the position of a transgene integrated into the mouse genome. The transgene maps to band F on the physical map of mouse chromosome 15 by in situ analysis and is tightly linked genetically to a cluster of loci that include the mutations caracul (Ca) and microcytic anemia (mk). Genetic analysis of the offspring of noninbred animals carrying the transgene and marker loci demonstrates a significant deficiency of homozygous progeny at weaning. When inbred mice heterozygous for the transgene are mated, about one-quarter of their offspring are homozygous; none of these animals survives more than 1 day after birth. It appears likely that a recessive insertional mutation has occurred as a result of transgene integration into a locus required for postnatal viability. We call this mutation transgenic perinatal lethality (Tg.ple).
authors.affiliation: Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts 02115.
authors.forename: D R
authors.initials: DR
authors.lastname: Beier
authors.forename: C C
authors.initials: CC
authors.lastname: Morton
authors.forename: A
authors.initials: A
authors.lastname: Leder
authors.forename: R
authors.initials: R
authors.lastname: Wallace
authors.forename: P
authors.initials: P
authors.lastname: Leder
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D005804:Genes, Lethal; D005808:Genes, Recessive; D005813:Genes, Synthetic; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D008822:Mice, Transgenic / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011995:Recombination, Genetic