Inhibitory effects of cadmium on rat ornithine carbamoyltransferase and urea synthesis in the rat liver parenchymal cells.
Ornithine carbamoyltransferase (OCTase) has been identified as a cadmium-binding protein in rat liver (Aoki et al., 1988), whose enzymatic activity is inhibited by cadmium and mercury ions. Cadmium is a competitive inhibitor of OCTase with respect to carbamoyl phosphate and a noncompetitive inhibitor with respect to ornithine. Data presented here show that in primary cultured rat liver parenchymal cells cadmium at concentrations of 7-20 microM inhibits urea production.
Aoki
Y
Y
National Institute for Environmental Studies, Ibaraki, Japan.
Suzuki
K T
KT
D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009954:Ornithine Carbamoyltransferase / Q000037:antagonists & inhibitors*; D051381:Rats; D011919:Rats, Inbred Strains; D014508:Urea / Q000096:biosynthesis*
D002104:Cadmium; D014508:Urea; D009954:Ornithine Carbamoyltransferase