title: Acute and chronic renal effects of radiocontrast in diabetic rats. Role of anesthesia and risk factors.
abstract: The acute and chronic renal effects of the intravenous injection of meglumine diatrizoate sodium 76% (CM), 5 mL/kg body weight were studied in diabetic (DM) and age-matched normal (C) female Sprague-Dawley rats. In acute studies, the effect of anesthesia was assessed for 2 hours. Although anesthesia decreased 14C-inulin clearance (Cin) in both DM and C rats (P less than .001 vs. conscious values), there was no impairment of Cin in either group after administration of CM. In chronic studies, creatinine clearance (Ccr) was followed for 3-4 days after CM administration. Four protocols to assess risk factors in DM and C were used: adult rats with normal hydration (2A); old dehydrated rats with DM of long duration (2B); rats with prior decreased Ccr (remnant kidney, 2C); and DM rats treated with insulin (2D). No clear-cut nephrotoxicity was apparent in these studies, except that proteinuria increased with CM in Study 2C. A greater severity of renal dysfunction, renal disease, or the association of multiple risk factors may be necessary to induce CM-related nephrotoxicity in the experimental animal. The rat, diabetic or not, may have an inherent resistance to CM-induced renal injury.
authors.affiliation: Medical Service, Veterans Administration Medical Center, Miami, Florida 33125.
authors.forename: C A
authors.initials: CA
authors.lastname: Vaamonde
authors.forename: R T
authors.initials: RT
authors.lastname: Bier
authors.forename: R
authors.initials: R
authors.lastname: Papendick
authors.forename: H
authors.initials: H
authors.lastname: Alpert
authors.forename: W
authors.initials: W
authors.lastname: Gouvea
authors.forename: B
authors.initials: B
authors.lastname: Owens
authors.forename: V
authors.initials: V
authors.lastname: Pardo
mesh_terms: D000375:Aging / Q000502:physiology; D000758:Anesthesia; D000818:Animals; D003681:Dehydration / Q000150:complications / Q000503:physiopathology; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000000981:diagnostic imaging / Q000503:physiopathology*; D003974:Diatrizoate Meglumine / Q000633:toxicity*; D005260:Female; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007444:Inulin; D007668:Kidney / Q000187:drug effects* / Q000503:physiopathology; D007677:Kidney Function Tests; D051381:Rats; D011919:Rats, Inbred Strains; D012307:Risk Factors; D014567:Urography
chemical_list: D003974:Diatrizoate Meglumine; D007444:Inulin