title: Effects of Chinese drugs "xiebai" and "dasuan" on human platelet aggregation (Allium bakeri, A. sativum).
abstract: Adenosine (1), guanosine (2), and tryptophan (3), as well as beta-sitosterol beta-D-glucoside (4) were isolated from the n-butanol-soluble fraction of Xiebai, the tuber of Allium bakeri Reg., which was recognized to have an anti-platelet aggregation effect. Moreover, 1 and 2 were also isolated from the n-butanol-soluble fraction of Dasuan, the tuber of A. sativum L. Compound 1 showed a significant inhibitory activity against both the primary and secondary wave aggregation of human platelet induced by 2 microM ADP, whereas compounds 2-4 showed no or very low inhibitory effects. The structure-activity relationships on human platelet aggregation with regard to 1, 2 and their derivatives, adenosine 2'-monophosphate (5), adenosine 5'-monophosphate (6), adenosine triphosphate (7), guanosine 3'-monophosphate (8), and guanosine 5'-monophosphate (9), and guanylyl(3'----5')adenosine (10) are discussed. Compounds 5-7 and 10 also inhibited the primary and secondary wave aggregation with a dose-dependent response.
authors.forename: T
authors.initials: T
authors.lastname: Okuyama
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: S
authors.initials: S
authors.lastname: Shibata
authors.forename: M
authors.initials: M
authors.lastname: Hoson
authors.forename: T
authors.initials: T
authors.lastname: Kawada
authors.forename: M
authors.initials: M
authors.lastname: Masaki
authors.forename: N
authors.initials: N
authors.lastname: Yamate
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives; D000490:Allium / Q000032:analysis*; D005591:Chemical Fractionation; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005737:Garlic / Q000032:analysis*; D010946:Plants, Medicinal; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D010975:Platelet Aggregation Inhibitors; D000241:Adenosine