Development of an in vitro model for the disruption of extracellular matrix components in the study of congenital renal parenchymal dysmorphism.
We attempted to refine a model system for the study of renal parenchymal malformations and demonstrate its use with a preliminary study of the induction of congenital renal dysmorphism. A technique is described whereby 3-day chick embryos are explanted from their shells and cultured in vitro in specially constructed polyethylene chambers. At 8 days of development, the time of ureteral induction of the metanephric mesenchyme, 6-diazo-5-oxo-norleucine, an inhibitor of proteoglycan biosynthesis, is administered as a single microinjection into the posterolateral body wall. Histological examination of fixed metanephric tissue revealed a gross deficiency in the number of differentiated tubules in norleucine-treated animals. A morphometric analysis was made of the actual area occupied by differentiated tubules in the norleucine-treated and control kidneys between 10 and 15 days of development. This analysis revealed a total tubule surface area of control kidneys of more than 2 times greater than that of embryos injected with norleucine at ureteral induction.
Howe
S C
SC
Department of Anatomy, Albany Medical College, Albany Medical Center, New York.
Cromie
W J
WJ
Kaye
N W
NW
D000014:Abnormalities, Drug-Induced / Q000196:embryology; D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo / Q000187:drug effects*; D005109:Extracellular Matrix / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000002:abnormalities*; D007684:Kidney Tubules / Q000002:abnormalities; D009646:Norleucine / Q000633:toxicity
D009646:Norleucine