A comparison of the phototoxicity of protoporphyrin, coproporphyrin and uroporphyrin using a cellular system in vitro.
The photobiological effects of protoporphyrin (PP), coproporphyrin (CP) and uroporphyrin (UP) were investigated using an in vitro model. Suspensions of Ehrlich ascites carcinoma cells were labelled with 51Cr and irradiated in the presence of a wide range of concentrations of PP, CP and UP. It was found that PP was the most potent photosensitizer in this system; CP was less effective than PP and UP was the least potent. The cell lysis by CP was enhanced by superoxide dismutase (SOD) and inhibited by catalase; the lysis by UP was also inhibited by catalase; on the other hand, the lysis by PP was not affected by SOD or catalase. These indicate that the cell lysis by CP and UP was largely due to hydrogen peroxide produced from superoxide formed during the irradiation. The lysis produced by PP was not mediated by hydrogen peroxide. These differences in the mechanisms of the phototoxicity of the various porphyrins may have relevance in the etiology and treatment of the porphyrias.
Aravind Menon
I
I
Department of Medicine, University of Toronto, Ontario, Canada.
Persad
S D
SD
Haberman
H B
HB
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002374:Catalase / Q000494:pharmacology; D003306:Coproporphyrins / Q000494:pharmacology*; D003624:Darkness; D008027:Light; D011166:Porphyrins / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014578:Uroporphyrins / Q000494:pharmacology*
D003306:Coproporphyrins; D011166:Porphyrins; D011524:Protoporphyrins; D014578:Uroporphyrins; D002374:Catalase; D013482:Superoxide Dismutase