Quantitative morphologic changes in nephron structures and urinary enzyme activity pattern in sodium-maleate-induced renal injury.
The morphologic changes in sodium-maleate-induced acute renal injury in the rat were quantified by a stereologic analysis. The major changes were confined to an increase in endocytic vacuoles and a decrease in mitochondrial inner membrane surface area. These results were found to be linked to significantly increased urinary activities of the cytosolic of the cytosolic enzymes fructose-1,6-bisphosphatase (FBP) and lactate dehydrogenase, the lysosomal enzyme N-acetyl-beta-glucosaminidase (NAG) and the NAD-dependent mitochondrial isocitrate dehydrogenase (ICDH). The highest increase was found for NAG, followed by FBP and ICDH.
Pfaller
W
W
Institute of Physiology, University of Innsbruck, Austria.
Joannidis
M
M
Gstraunthaler
G
G
Kotanko
P
P
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D004798:Enzymes / Q000652:urine*; D005919:Glomerular Filtration Rate; D007674:Kidney Diseases / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology*; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008298:Maleates / Q000633:toxicity*; D008928:Mitochondria / Q000201:enzymology / Q000648:ultrastructure; D009399:Nephrons / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism
D004798:Enzymes; D008298:Maleates