Isolation and purification of protein kinase C from human leukemia ML-1 cells phosphorylation of human leukemia RNA polymerase II in vitro.
Protein kinase C (PKC) was purified to near homogeneity from human leukemia ML-1 cells. The purified enzyme showed a single polypeptide band of 80 kDa on SDS-polyacrylamide gel after electrophoresis, and was totally dependent on Ca2+/phospholipid for activity. Diacylglycerol and the tumor-promoting on Ca2/phospholipid for activity. Diacylglycerol and the tumor-promoting phorbol esters stimulated the enzyme activity. Autophosphorylation of PKC purified from phenyl-Sepharose column showed both 80- and 37 kDa polypeptides. Further fractionation of PKC on a hydroxyapatite column revealed two peaks of enzyme activity, indicating that there may be two different forms of protein kinase C present in human leukemia cells. The purified PKC was used to phosphorylate RNA polymerase II of human leukemia cells in vitro and the autoradiogram showed that RNA polymerase II large subunits (240, 220 and 150 kDa) were phosphorylated in a time-dependent manner.
Chuang
L F
LF
Department of Pharmacology, School of Medicine, University of California, Davis 95616.
Zhao
F K
FK
Chuang
R Y
RY
D001345:Autoradiography; D002122:Calcium Chloride / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D004075:Diglycerides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007938:Leukemia / Q000201:enzymology; D010718:Phosphatidylserines / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000302:isolation & purification* / Q000378:metabolism; D012319:RNA Polymerase II / Q000302:isolation & purification* / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000201:enzymology*
D004075:Diglycerides; D010718:Phosphatidylserines; D011493:Protein Kinase C; D012319:RNA Polymerase II; D002122:Calcium Chloride