title: Transcriptional regulation of type X collagen during chondrocyte maturation.
abstract: The 18-day embryonic chick sternum expresses type X collagen only in the cephalic, presumptive mineralization zone which consists primarily of hypertrophic chondrocytes. The smaller chondrocytes of the caudal, permanent cartilaginous zone do not express this protein. Run-off transcriptions of nuclei isolated directly from cephalic and caudal 18-day embryonic sterna demonstrate that type X collagen is specifically transcribed by cells of the cephalic zone. In addition, type X mRNA is localized to the cephalic zone as detected by in situ hybridization. The results suggest that the cell-specific expression of the type X collagen gene is due entirely to transcriptional regulation.
authors.affiliation: Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115.
authors.forename: P
authors.initials: P
authors.lastname: LuValle
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: B R
authors.initials: BR
authors.lastname: Olsen
mesh_terms: D000818:Animals; D002356:Cartilage / Q000196:embryology* / Q000378:metabolism; D002642:Chick Embryo; D003094:Collagen / Q000235:genetics*; D015342:DNA Probes; D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D003094:Collagen