[Use of the DNA-DNA hybridization method on filters for determining the toxic potency of atypical strains of Vibrio cholerae isolated from natural sources].
The methods of the radioimmunoassay and the blot hybridization of restricted fragments of chromosomal DNA have been used for the characterization of V. cholerae atypical strains isolated from the natural environment. For all strains under study, the radioimmunoassay has been found to yield the most sharply defined data characterizing their atoxigenicity. The absence of the structural genes of toxin in the chromosomes of these strains has been shown by the method of blot hybridization. Some methodological simplifications of blot hybridization, having no adverse effect on the sensitivity of this method, have been tested.
Demkin
V V
VV
Brukhanskii
G V
GV
Zakharenko
V I
VI
Evdokimova
N M
NM
Gaĭlonskaia
I N
IN
D001427:Bacterial Toxins / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D011863:Radioimmunoassay; D014734:Vibrio cholerae / Q000378:metabolism*
D001427:Bacterial Toxins; D004269:DNA, Bacterial
Ispol'zovanie metoda DNK-DNK-gibridizatsii na fil'trakh dlia opredeleniia toksigennoĭ potentsii atipichnykh shtammov Vibrio cholerae, vydelennykh iz prirodnykh istochnikov.