[Experimental and clinical evaluations of flow cytometric DNA-aneuploidy for the determining malignancy of head and neck tumors].
Using flow cytometry, basic experiments and clinical trials to investigate DNA aneuploidy were performed. The site of the peak in the DNA histogram was found to be unstable in the flow cytometric assay. However, relatively stable DNA indices can be obtained if only internal standards, such as human lymphocytes, etc., are added to the samples, thus results in a more precise detection of aneuploidy. In 11 cases of head and neck tumors, DNA aneuploidy was observed in six out of seven cases that were histopathologically diagnosed as being malignant tumors, showing a correlation with the pathological studies as a whole. Further clinical studies may prove that the present method can be useful for the pathological, supplementary diagnostic and prognostic evaluations.
Oka
S
S
Dept. of Otorrhinolaryngology, Kansai Medical Univ.
Wada
Y
Y
Yamazaki
N
N
Yamashita
T
T
Kumazawa
T
T
Kawamoto
K
K
D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D011237:Predictive Value of Tests; D011379:Prognosis; D012015:Reference Standards
D004273:DNA, Neoplasm