Quantitative determination of 4-hydroxy-4-androstene-3,17-dione (4-OHA), a potent aromatase inhibitor, in human plasma, using isotope dilution mass spectrometry.
An original method is described for the determination in human plasma of 4-hydroxy-4-androstene-3,17-dione (4-OHA), a potent aromatase inhibitor, by isotope dilution mass-spectrometry using 7,7-[2H2]-4-OHA as internal standard. This compound was synthesized starting from 7,7-[2H2]-4-androstene-3,17-dione. The procedure includes an extraction step using an Extrelut 1 column and a derivatization with N,o-bis(trimethylsilyl)trifluoroacetamide (BSTFA). The minimum detection level of the method is 0.650 pg and the coefficients of variation for the 0.5 ng/ml (plasma) and 5 ng/ml (plasma) concentrations are 3.2% (within assay) and 6.7% (between assay) and 1.86% (within assay) and 2.3% (between assay) respectively.
Guarna
A
A
Department of Organic Chemistry, University of Florence, Italy.
Moneti
G
G
Prucher
D
D
Salerno
R
R
Serio
M
M
D000735:Androstenedione / Q000031:analogs & derivatives* / Q000097:blood; D047072:Aromatase Inhibitors; D002849:Chromatography, Gas; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods
D047072:Aromatase Inhibitors; D000735:Androstenedione; C014594:formestane