Demonstration of delta rec-pseudo J alpha rearrangement with deletion of the delta locus in a human stem-cell leukemia.
It has been hypothesized that a rearrangement between the delta recombining element (delta Rec) and a pseudo J alpha gene serves to delete the TCR-delta locus before rearrangement of the TCR-alpha genes. We have now sequenced a direct, site-specific rearrangement between the delta Rec element and a pseudo J alpha gene in a human leukemic stem-cell line. Putative "N-sequence" addition was noted at the site of recombination, suggesting that this event occurred at a time when the enzyme(s) involved in N-region addition were active in this cell. This provides support for the view that deletion of the TCR-delta locus is required before rearrangement of the TCR-alpha chain genes.
Begley
C G
CG
Navy Medical Oncology, National Cancer Institute, Bethesda, Maryland 20892.
Aplan
P D
PD
Davey
M P
MP
de Villartay
J P
JP
Cohen
D I
DI
Waldmann
T A
TA
Kirsch
I R
IR
D000208:Acute Disease; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002872:Chromosome Deletion; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000235:genetics*; D007938:Leukemia / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D007133:Immunoglobulin Joining Region; D011948:Receptors, Antigen, T-Cell