title: Expression of the c-fos protooncogene by human and murine erythroblasts.
abstract: The expression of the c-fos protooncogene was investigated by in situ hybridization in normal murine bone marrow cells. A strong signal was found in murine marrow cells having the morphologic features of erythroblasts. This result was confirmed in human marrow cells using a double labeling technique (in situ hybridization and immunocytochemistry). A majority (70%) of the cells expressing c-fos mRNA were glycophorin A-positive. In contrast, granulocytic precursors (CD 15-positive) or monocytes and their precursors (CD 14-positive cells) did not significantly hybridize with the c-fos probe. In addition, c-fos mRNA (2.2Kb) was detected by Northern blotting in RNA extracted from homogeneous populations of erythroblasts obtained by immune panning from fetal liver and from adult blood BFU-E-derived colonies. Fos protein was also detected in erythroblasts by immunofluorescence. The high level of c-fos mRNA previously found in hematopoietic tissue should therefore be related to the transcription of the c-fos gene during terminal erythroid differentiation.
authors.affiliation: INSERM U.91, Hôpital Henri Mondor, Créteil, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Caubet
authors.forename: M T
authors.initials: MT
authors.lastname: Mitjavila
authors.forename: A
authors.initials: A
authors.lastname: Dubart
authors.forename: D
authors.initials: D
authors.lastname: Roten
authors.forename: S C
authors.initials: SC
authors.lastname: Weil
authors.forename: W
authors.initials: W
authors.lastname: Vainchenker
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000032:analysis*; D015342:DNA Probes; D004900:Erythroblasts / Q000032:analysis*; D005333:Fetus; D005914:Globins / Q000032:analysis; D006021:Glycophorins / Q000032:analysis; D006801:Humans; D008099:Liver / Q000032:analysis; D051379:Mice; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D015342:DNA Probes; D006021:Glycophorins; D012333:RNA, Messenger; D005914:Globins