Rate of interaction of IgG1 and IgG3 sensitized red cells with monocytes in the phagocytosis assay.
Another aspect of the preferential binding to monocytes of IgG3-coated erythrocytes over IgG1-coated erythrocytes has been demonstrated by measuring the rate of interaction in the phagocytosis assay. Erythrocytes sensitized with comparable numbers of IgG1 and IgG3 monoclonal and polyclonal anti-D antibodies were incubated with a monocyte preparation for up to 2 h. Interaction with the erythrocytes sensitized with monoclonal or polyclonal IgG3 was much more rapid than with erythrocytes sensitized with IgG1. With IgG3-sensitized erythrocytes maximum interaction was found after 30 min, whereas with IgG1-sensitized erythrocytes 2 h were required for maximum interaction. These results are consistent with the longer hinge region of IgG3 allowing bridges to be formed more easily between the negatively charged erythrocytes and monocytes.
Brojer
E
E
Institute of Haematology, Warsaw, Poland.
Merry
A H
AH
Zupanska
B
B
D000906:Antibodies; D000911:Antibodies, Monoclonal; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000502:physiology*; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis; D011971:Receptors, Immunologic / Q000502:physiology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011971:Receptors, Immunologic