[Synthesis of 5'-phosphorylated oligodeoxyribonucleotides by the H-phosphonate method].
N,O-protected 2'-deoxyribonucleotide-3'-H-phosphonates and 2-(p-nitrophenylethyl)-H-phosphonate were prepared and used in solid-phase synthesis of 5'-phosphorylated DNA. H-Phosphonate condensation is performed with 1:2 ratio of P-component to activator (pivaloyl chloride). Protection groups were removed either by sequential treatment with conc. NH3 and DBU or by combination of these reagents.
Filippov
S A
SA
Esipov
D S
DS
Kalinichenko
S V
SV
Dobrynin
V N
VN
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D010766:Phosphorylation
D009838:Oligodeoxyribonucleotides
Sintez 5'-fosforilirovannykh oligodezoksiribonukleotidov H-fosfonatnym metodom.