Echinococcus multilocularis: inhibition of murine neutrophil and macrophage chemotaxis.
Resident peritoneal neutrophils and macrophages from mice infected with 50 +/- 5 cysts of Echinococcus multilocularis were collected at 2, 4, 6, 8, 10, and 16 weeks postinfection. Their ability to respond and migrate to purified parasite larval antigens or endotoxin-activated mouse serum (EAMS) in comparison to normal peritoneal cells from uninfected mice was tested in vitro using Boyden chambers. Early in the infection, both cell types responded to the specific and nonspecific chemoattractants as the control group. However, at 8 and 10 weeks postinfection, the neutrophils and macrophages lost their response to parasite antigens but retained their ability to migrate to EAMS. Toward the 12th and 16th week postinfection, both cell types lost their ability to migrate to the specific as well as the nonspecific factors. The data presented suggest that the cellular mechanisms of recognition and chemotaxis in mice infected with alveolar hydatid cysts are impaired.
Alkarmi
T
T
Department of Microbiology, Faculty of Medicine, University of Kuwait, Safat.
Behbehani
K
K
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002633:Chemotaxis; D002634:Chemotaxis, Leukocyte; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology*
D000947:Antigens, Helminth