title: 31P NMR studies on rejuvenation of outdated red blood cells: complete regeneration of ATP is accompanied by partial Mg-ATP recomplexation.
abstract: We used 31P Nuclear Magnetic Resonance (NMR) spectroscopy to investigate whether regeneration of ATP in outdated human blood is accompanied by Mg-ATP recomplexation. We found that the loss of 'total ATP' (i.e. the sum of ATP4- + MG-ATP2-) which occurs during blood storage is completely reversible by treatment of RBC with precursors for purine nucleotide salvage. The magnesium-ATP complex, however, is only partially (70%) recovered due to a continuous decline of cytosolic free Mg2+ with increasing RBC age. Total RBC magnesium, as measured by flame absorption spectroscopy, did not show any significant change under these conditions.
authors.affiliation: Department of Animal Physiology, Gorlaeus Laboratories, University of Leiden, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: van Waarde
authors.forename: A
authors.initials: A
authors.lastname: Lombarts
authors.forename: G
authors.initials: G
authors.lastname: van den Thillart
authors.forename: C
authors.initials: C
authors.lastname: Erkelens
authors.forename: J
authors.initials: J
authors.lastname: Lugtenburg
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood*; D001793:Blood Preservation; D003080:Cold Temperature; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy
chemical_list: D000255:Adenosine Triphosphate; D008274:Magnesium