title: [Measurement of the concentration of free cytoplasmic calcium in the process of platelet aggregation using a fluorescent method].
abstract: A multiwavelength method for measuring free cytosolic calcium concentration is proposed. It is based on the registration of the fluorescent spectrum of calcium--sensitive probe indo-1 and deconvolution of the spectrum into components corresponding to free and bound forms of the probe. Calcium concentration is calculated as a product of calcium-probe dissociation constant by calcium-bound to free form concentration ratio. The obtained values are independent of variations in light-scattering properties of the medium and total dye concentration in the optical channel. It is shown that during ADP-induced platelet aggregation calcium concentration rises without measurable delay after the addition of the inducer and significantly decreases by the time the aggregation begins.
authors.forename: E G
authors.initials: EG
authors.lastname: Popov
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Gavrilov
authors.forename: E Ia
authors.initials: EIa
authors.lastname: Pozin
authors.forename: Z A
authors.initials: ZA
authors.lastname: Gabbasov
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D005456:Fluorescent Dyes; D008027:Light; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D000244:Adenosine Diphosphate; D002118:Calcium
vernacular_title: Izmerenie kontsentratsii svobodnogo tsitoplazmaticheskogo kal'tsiia v protsesse agregatsii trombotsitov fluorestsentnym metodom.