title: The polymerase chain reaction and its applications.
abstract: The advent of recombinant DNA technology has brought us many new techniques for the detection of genetic defects. With these methods it has become possible to do prenatal screening of the DNA of fetuses and to detect genetic defects that would lead to congenital disease. The biggest problem facing researchers has been the limited sensitivity of the techniques of molecular biology especially in detecting small changes in the DNA.
authors.forename: A
authors.initials: A
authors.lastname: Milbourne
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: J M
authors.initials: JM
authors.lastname: Nakamura
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D000755:Anemia, Sickle Cell / Q000175:diagnosis; D004259:DNA-Directed DNA Polymerase; D006801:Humans; D011296:Prenatal Diagnosis / Q000379:methods
chemical_list: D004259:DNA-Directed DNA Polymerase