An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cells.
We have used the cholesterol auxotrophy of NS-1 mouse myeloma cells as the basis for selecting NS-1 hybridomas. The outgrowth of nascent NS-1 hybridomas in cholesterol-free serum-free medium was 3- to 9-fold more efficient than that in HAT medium and resulted in 3- to 13-times as many antigen-reactive hybridoma wells. This method of hybridoma selection can be applied with any sterol-dependent parent cell line. Hybridomas established under serum-free culture conditions were growth inhibited by fetal calf serum.
Myoken
Y
Y
Department of Oral and Maxillofacial Surgery I, Hiroshima University School of Dentistry, Japan.
Okamoto
T
T
Osaki
T
T
Yabumoto
M
M
Sato
G H
GH
Takada
K
K
Sato
J D
JD
D000630:Aminopterin / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002784:Cholesterol / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D006825:Hybridomas / Q000473:pathology*; D007042:Hypoxanthines / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology*; D013936:Thymidine / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D007042:Hypoxanthines; D002784:Cholesterol; D000630:Aminopterin; D013936:Thymidine