Determination of soluble fibrin by turbidimetry of its protamine sulphate-induced paracoagulation.
Protamine sulphate-induced aggregation of soluble fibrin causes an increase of turbidity in the plasma sample, which can be measured by means of kinetic turbidimetry. A method was developed which is sufficiently sensitive for the determination of soluble fibrin in plasma without interfering with the sensitivity for fibrinogen. The performance of the assay was studied by analysing plasma samples with high concentrations of fibrinogen and soluble fibrin at different pH values, at different concentrations of plasma and protamine sulphate, and using different wavelengths and analysis times. Measurement of thrombin-induced fibrinogen-fibrin-transformation by the developed turbidimetric method, gave results that correlated well with the release of fibrinopeptide A. The new protamine sulphate method for turbidimetric determination of soluble fibrin is characterized by its practicability, rapid availability of reproducible, quantitative results, and its economy of reagents and time in single and serial analysis. Therefore it seems well-suited for the routine diagnosis of hypercoagulability with increased fibrinogen turnover.
Wieding
J U
JU
Hämatologie, Universitätsklinik, Göttingen, FRG.
Eisinger
G
G
Köstering
H
H
D001777:Blood Coagulation / Q000187:drug effects*; D005337:Fibrin / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007700:Kinetics; D009391:Nephelometry and Turbidimetry; D011479:Protamines / Q000494:pharmacology*; D012995:Solubility; D013053:Spectrophotometry
D007202:Indicators and Reagents; D011479:Protamines; D005337:Fibrin