Novel preparation of zein microspheres conjugated with PS-K available for cancer immunotherapy.
Microspheres which entrapped PS-K were prepared using Zein as a carrier matrix by a one-step or two-step process in dimethyl sulfoxide-H2O media. Microspheres prepared by the latter process provided satisfactory recovery and uptake of PS-K. Use of a catalytic amount of dl-camphorsulfonic acid, glutaraldehyde and rapid addition of aqueous solution of polyvinylpyrrolidone into the reaction media are crucial for the preparation of fine and mono-dispersed microspheres. The release rate of PS-K could be controlled by altering the ratio of PS-K to Zein and the conditions of the medium. In the presence of actinase E, drug release was considerably increased to 70-80% after 24 h incubation. Sonication of the aggregated microspheres readily yielded a mono-dispersed preparation with a particle diameter of less than 1 micron, which would be a suitable size for phagocytosis by macrophages.
Matsuda
Y
Y
Suzuki
T
T
Sato
E
E
Sato
M
M
Koizumi
S
S
Unno
K
K
Kato
T
T
Nakai
K
K
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004337:Drug Carriers; D007167:Immunotherapy; D008863:Microspheres; D011509:Proteoglycans / Q000008:administration & dosage*; D015028:Zein
D004337:Drug Carriers; D011509:Proteoglycans; C010770:polysaccharide-K; D015028:Zein