title: Expression of proliferation and differentiation antigens in response to modulation of membrane fluidity in chronic lymphocytic leukemia lymphocytes.
abstract: Isolated lymphocytes from patients with chronic lymphocytic leukemia (CLL) and from healthy controls were investigated for cell membrane fluidity and lipid phase separation. Expression of surface immunoglobulins, OKB7 and HLA-DR antigen, transferrin and interleukin-2 receptor was determined in relation to modulation of membrane lipid composition in each individual case. Structural and functional changes of membrane lipids and impaired lipid-protein interactions could be demonstrated in CLL lymphocytes. However, there is no simple correlation of membrane fluidity and receptor expression. This interaction poses a complex physicochemical problem, which must consider the original structural and functional state of the lipid bilayer. Disturded lipid-protein interactions are discussed in terms of defective transmembrane signalling and cell-cell interaction, allowing uncoupling of the leukemic cell from the network of growth and differentiation factors.
authors.affiliation: Immunopathology Section, University of Cologne, FRG.
authors.forename: S
authors.initials: S
authors.lastname: Daefler
authors.forename: G R
authors.initials: GR
authors.lastname: Krueger
mesh_terms: D000943:Antigens, Differentiation / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002788:Cholesterol Esters / Q000494:pharmacology; D005454:Fluorescence Polarization; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000503:physiopathology; D008560:Membrane Fluidity / Q000187:drug effects; D010713:Phosphatidylcholines / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011990:Receptors, Transferrin / Q000032:analysis
chemical_list: D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D002788:Cholesterol Esters; D006684:HLA-DR Antigens; D010713:Phosphatidylcholines; D011947:Receptors, Antigen, B-Cell; D011990:Receptors, Transferrin