A prostaglandin oligomeric derivative inhibits activities of phospholipase and protease: a possible mechanism of membrane protection during ischemia.
A prostaglandin oligomeric derivative was synthesized by alkaline treatment of prostaglandin E1. This compound protected the perfused rat heart from global ischemia. This compound was found to inhibit several lipolytic and proteolytic enzymes in vitro. When phospholipase A2 from Naja naja venom was used as an enzyme and phosphatidylcholine was used as a substrate, 50 per cent inhibition was achieved at 50 microM of the prostaglandin derivative. When trypsin and casein were used as enzyme and substrate, 50 per cent inhibition was obtained at 80 microM. A possible mechanism of beneficial effect of this compound in protecting membranes during ischemia is discussed.
Ohnishi
S T
ST
Membrane Research Institute, University City Science Center, Philadelphia, PA 19104.
Katsuoka
M
M
Hidaka
S
S
D000818:Animals; D003327:Coronary Disease / Q000201:enzymology / Q000517:prevention & control*; D066298:In Vitro Techniques; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D011453:Prostaglandins / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D051381:Rats; D014361:Trypsin Inhibitors
D011453:Prostaglandins; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; C060277:MR 256; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2