Sequence selective binding of ditrisarubicin B to DNA: comparison with daunomycin.
DNase I footprinting has been used to examine the sequence selective binding of ditrisarubicin B, a novel anthracycline antibiotic, to DNA. At 37 degrees C no footprinting pattern is observed, the drug protects all sites from enzymic cleavage with equal efficiency. At 4 degrees C a footprinting pattern is induced with low drug concentrations which is different from that produced by daunomycin. The best binding sites contain the dinucleotide step GpT (ApC) and are located in regions of alternating purines and pyrimidines.
Fox
K R
KR
Dept of Physiology & Pharmacology, University of Southampton, England.
Kunimoto
S
S
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D003630:Daunorubicin / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004587:Electrophoresis, Agar Gel; D007202:Indicators and Reagents; D009710:Nucleotide Mapping
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D007202:Indicators and Reagents; C039539:ditrisarubicin B; D004247:DNA; D003850:Deoxyribonuclease I; D003630:Daunorubicin