title: Androgen and estrogen effects on protein synthesis by the adult rabbit epididymis.
abstract: The effects of castration and hormone replacement on [35S]methionine incorporation into newly synthesized proteins by the adult rabbit epididymis were studied in vitro. The proteins were analyzed using two-dimensional polyacrylamide gel electrophoresis. Short term (4-day) castration resulted in a few changes in the pattern of radiolabeled proteins observed in the caput, but no effect was seen in the corpus or cauda. The changes in the caput could be reversed if the samples were incubated with testosterone. The epididymis of short term castrates failed to respond to exogenous estradiol. Long term castration (4-6 weeks) resulted in changes in protein synthesis among all three epididymal segments. Short term (4-h) incubation with testosterone restored the pattern of proteins secreted by the caput and cauda to that in intact rabbits. Short term incubation with estradiol did not restore the pattern of radiolabeled secreted proteins, but it did slightly intensify a 28K protein (pI 5.2) that was present in the caput and cauda of castrated animals. No clear-cut effect of the hormones on proteins secreted by the corpus was observed. Short term incubation with testosterone or estradiol restored the patterns of tissue proteins synthesized by the caput and corpus of castrated rabbits to that in intact animals. In the cauda, estradiol also enhanced the presence of a small group of high mol wt proteins present in the control castrate sample, while testosterone inhibited these proteins. This group of proteins was absent in cauda tissue samples from intact rabbits.
authors.affiliation: Department of Obstetrics and Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: T W
authors.initials: TW
authors.lastname: Toney
authors.forename: B J
authors.initials: BJ
authors.lastname: Danzo
mesh_terms: D000728:Androgens / Q000502:physiology*; D000818:Animals; D003470:Culture Media; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology*; D008297:Male; D009919:Orchiectomy; D014176:Protein Biosynthesis; D011817:Rabbits; D012741:Sexual Maturation; D013739:Testosterone / Q000494:pharmacology
chemical_list: D000728:Androgens; D003470:Culture Media; D004967:Estrogens; D013739:Testosterone; D004958:Estradiol