Accurate and efficient RNA polymerase III transcription in a cell-free extract prepared from Ascaris suum embryos.
High-speed supernatant (S100) extracts derived from homogenized Ascaris suum embryos efficiently transcribe added RNA polymerase III templates including cloned 5S rRNA genes of the filarial parasite Brugia malayi. Several criteria, including two-dimensional RNase T1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated.
Maroney
P A
PA
Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH.
Hannon
G J
GJ
Nilsen
T W
TW
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001200:Ascaris / Q000235:genetics*; D002009:Brugia / Q000235:genetics*; D002474:Cell-Free System; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005260:Female; D009693:Nucleic Acid Hybridization; D012320:RNA Polymerase III / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000096:biosynthesis; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014981:Xenopus / Q000235:genetics
D000546:Amanitins; D012341:RNA, Ribosomal, 5S; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III