Immunohistochemical techniques for detection of dermatan sulfate proteoglycan in tissue sections.
Dermatan sulfate proteoglycan chains were detected in tissue sections treated with chondroitin B-lyase (0.01 units/ml) in 20 mM Tris-HCl (pH 8.0) for 1 hr, followed by staining with antibody 9A2 specific for unsaturated uronic acid coupled to N-acetylgalactosamine-4 sulfate. In contrast, after treatment with chondroitin B-lyase, no positive staining was observed with antibodies 3B3 and 1B5 which react to the unsaturated uronic acid coupled to N-acetylgalactosamine 6-sulfate and unsaturated uronic acid coupled to N-acetylgalactosamine, respectively. The distribution of dermatan sulfate thus revealed was confirmed by comparison with that found by monoclonal antibody 6B6 which reacts with small proteoglycans carrying dermatan sulfate side chains. The localization of positive staining in fibrous connective tissues was almost identical with these two procedures.
Sobue
M
M
Department of Laboratory Medicine, Nagoya University School of Medicine, Japan.
Takeuchi
J
J
Fukatsu
T
T
Nagasaka
T
T
Nakashima
N
N
Ogura
T
T
Katoh
T
T
Yoshida
K
K
D000911:Antibodies, Monoclonal; D002807:Chondroitin / Q000031:analogs & derivatives*; D002808:Chondroitin Lyases; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D003871:Dermatan Sulfate / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D011509:Proteoglycans / Q000032:analysis*; D012867:Skin / Q000032:analysis* / Q000166:cytology
D000911:Antibodies, Monoclonal; D011508:Chondroitin Sulfate Proteoglycans; D011509:Proteoglycans; C032649:dermatan sulfate proteoglycan; D003871:Dermatan Sulfate; D002807:Chondroitin; D002808:Chondroitin Lyases