title: Manipulating the intracellular environment of hippocampal slices: pH and high-energy phosphates.
abstract: The intracellular energetic environment of rat hippocampal slices was manipulated by bolstering ATP levels following the addition of adenosine to the incubation medium, or by manipulating intracellular pH. Addition of 8 mM adenosine to the incubation medium increased total tissue adenylate and ATP content, but did not prolong electrical function during anoxia. Further, it resulted in long-lasting alterations in normoxic evoked responses. Intracellular pH (pHi) was changed by manipulating the bicarbonate/CO2 ratio of the incubation medium, or by adding amiloride, a hydrogen/sodium antiport blocker. Estimates of intracellular pH using the creatine kinase equilibrium agree with those obtained by Neutral red scanning spectrophotometry in control conditions. However, only Neutral red indicated an acidification with amiloride treatment, while the creatine kinase equilibrium was preferentially affected by hypercapnia, suggesting the presence of at least two pH compartments in hippocampal brain slices. These manipulations cannot be carried out easily in vivo, and provide a means of determining the importance of metabolic changes on neural function during anoxia.
authors.affiliation: Department of Oral Biology, Case Western Reserve University School of Dentistry, Cleveland, OH 44106.
authors.forename: T S
authors.initials: TS
authors.lastname: Whittingham
authors.forename: E
authors.initials: E
authors.lastname: Warman
authors.forename: H
authors.initials: H
authors.lastname: Assaf
authors.forename: T J
authors.initials: TJ
authors.lastname: Sick
authors.forename: J C
authors.initials: JC
authors.lastname: LaManna
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000227:Adenine Nucleotides / Q000378:metabolism*; D000241:Adenosine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008867:Microtomy; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate; D000241:Adenosine