title: Maintenance of neuronal expression of calbindin by a muscular extract in cultures of chick dorsal root ganglion cells.
abstract: Calbindin D-28K is a calcium-binding protein which is expressed by subpopulations of dorsal root ganglion cells cultured from 10-day-old (E10) chick embryos. After 7 or 10 days of culture, more than 20% of the ganglion cells are immunostained by an anticalbindin-antiserum; however, after 14 days of culture, the proportion drops to 10%. This fall can be prevented by addition of muscle extract to cultures at 10 days. Thus the transitory expression of calbindin-immunoreactivity by responsive sensory neurons would be not only induced but also maintained by a differentiation factor of muscular origin.
authors.affiliation: Institut d'Histologie et d'Embryologie, Université de Lausanne, Switzerland.
authors.forename: I
authors.initials: I
authors.lastname: Barakat
authors.forename: B
authors.initials: B
authors.lastname: Droz
mesh_terms: D000818:Animals; D064026:Calbindins; D002478:Cells, Cultured; D002642:Chick Embryo; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D009132:Muscles / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D014020:Tissue Extracts