Cloning of the amiA locus of Streptococcus pneumoniae and identification of its functional limits.
Mutations in the amiA locus of the Gram-positive bacterium Streptococcus pneumoniae confer a complex phenotype including resistance to various antineoplastic drugs. As a first step towards the understanding of the molecular organization and the function(s) of this locus, we have cloned DNA fragments carrying its 5'- and 3'-extremities. We have isolated and characterized a down-promoter mutation and have located the functional limits of the locus. The amiA locus is between 5.8 and 7.5 kb long strongly suggesting that it encodes several proteins.
Alloing
G
G
Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS, Université Paul Sabatier, Toulouse, France.
Trombe
M C
MC
Claverys
J P
JP
D000630:Aminopterin; D000970:Antineoplastic Agents; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*
D000970:Antineoplastic Agents; D004269:DNA, Bacterial; D000630:Aminopterin