title: Cholesterol esterification as a limiting factor in accumulation of cell cholesterol: a comparison of two J774 macrophage cell lines.
abstract: It was previously reported by Tabas et al. that J774 macrophages, unlike mouse peritoneal macrophages, accumulate large amounts of cholesteryl esters when incubated with native low-density lipoprotein (LDL). Comparison of the cell line (designated J774A.2) used in those experiments with its parent line (J774A.1) indicates that it is a variant with a greater rate of cholesterol esterification. This large difference in cholesterol esterification was accompanied by only a small difference in rates of LDL uptake and degradation by the J774A.2 line. The J774A.2 cells have become a variant line through either mutation or selection which has enhanced its susceptibility to foam cell formation by its markedly increased ability to esterify cholesterol.
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla 92093-0613.
authors.forename: J C
authors.initials: JC
authors.lastname: Khoo
authors.forename: E
authors.initials: E
authors.lastname: Miller
authors.forename: P
authors.initials: P
authors.lastname: McLoughlin
authors.forename: I
authors.initials: I
authors.lastname: Tabas
authors.forename: W J
authors.initials: WJ
authors.lastname: Rosoff
mesh_terms: D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D008078:Cholesterol, LDL / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004951:Esterification; D014644:Genetic Variation; D002785:Sterol O-Acyltransferase / Q000378:metabolism
chemical_list: D008078:Cholesterol, LDL; D002784:Cholesterol; D003513:Cycloheximide; D002785:Sterol O-Acyltransferase