[The micronuclei detection test in stimulated human peripheral lymphocytes using cytochalasin B].
The authors introduced a technique for assessment of cells with micronuclei in stimulated human peripheral lymphocytes using cytochalasin B (CB technique). The spontaneous level of cells with micronuclei was assessed in an ageing population group. The group comprised fifteen subjects (12 women and 3 men), mean age 79.7 +/- 6.7 years. The spontaneous level of cells with micronuclei was 33.7 per 1000 evaluated cells, i.e. 3.37%. The authors consider the possibility of introducing the test for assessment of micronuclei in human peripheral lymphocytes using cytochalasin B as an alternative test in the cytogenetic analysis of chromosomal aberrations in human peripheral lymphocytes.
Kocisová
J
J
Srám
R J
RJ
D000368:Aged; D000369:Aged, 80 and over; D003571:Cytochalasin B / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D008875:Middle Aged
D003571:Cytochalasin B
Test na stanovení mikrojader ve stimulovaných lidských periferních lymfocytech za vyuzití cytochalasinu B.