title: Purification and partial characterization of human polyamine synthases.
abstract: Spermidine synthase was purified to apparent homogeneity from human spleens (8700-fold) by affinity chromatography. The native enzyme was composed of two subunits of identical Mr (35,000) and showed an apparent Mr of 62,000 in pore-gradient gel electrophoresis. Its pI was 5.1, Spermine synthase was purified to apparent homogeneity from placenta (5300-fold) and from kidney (4600-fold). The native enzyme was composed of two subunits of identical Mr (45,000) and showed an apparent Mr of 78,000 in pore-gradient gel electrophoresis. In isoelectric focusing it revealed two bands, with pI values of 4.9 and 5.0. Both synthases were present in all human tissues studied, but revealed a clear tissue-specific pattern. Mouse antisera against spermidine synthase revealed only one band, of Mr 35,000, in all purified enzyme preparations and in crude human tissue extracts in immunoblotting. Antisera against spermine synthase showed an immunoreactive band corresponding to the Mr of the subunit of spermine synthase. These antisera did not indicate any cross-reactivity in immunoblotting. Thus spermine synthase and spermidine synthase do not share homologous antigenic sites and are totally different proteins.
authors.affiliation: Department of Biochemistry, University of Kuopio, Finland.
authors.forename: E O
authors.initials: EO
authors.lastname: Kajander
authors.forename: L I
authors.initials: LI
authors.lastname: Kauppinen
authors.forename: R L
authors.initials: RL
authors.lastname: Pajula
authors.forename: K
authors.initials: K
authors.lastname: Karkola
authors.forename: T O
authors.initials: TO
authors.lastname: Eloranta
mesh_terms: D015153:Blotting, Western; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D007700:Kinetics; D011436:Spermidine Synthase / Q000302:isolation & purification* / Q000378:metabolism; D013097:Spermine Synthase / Q000302:isolation & purification* / Q000378:metabolism; D014166:Transferases / Q000302:isolation & purification*
chemical_list: D014166:Transferases; D011436:Spermidine Synthase; D013097:Spermine Synthase