Wavelength dependence of DNA incision by a human ultraviolet endonuclease.
The wavelength dependence of an ultraviolet irradiation of the DNA substrate for a human endonuclease was determined. Sites of DNA incision for all UVB and UVC wavelengths examined were at cytosines which were neither cyclobutane pyrimidine dimers nor 6-4'-(pyrimidin-2-one)pyrimidines. The optimal wavelengths for formation of these cytosine photoproducts were between 270 and 295 nm. This human endonuclease therefore has a similar ultraviolet substrate specificity to endonuclease III.
Gallagher
P E
PE
Weiss
R B
RB
Brent
T P
TP
Duker
N J
NJ
D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000528:radiation effects*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D009097:Multienzyme Complexes / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity; D014466:Ultraviolet Rays
D009097:Multienzyme Complexes; C032219:UV endonuclease; D004247:DNA; D004706:Endodeoxyribonucleases; D009699:N-Glycosyl Hydrolases