[Expression of immunoglobulin genes and T lymphocyte antigen receptors in a case of acute lymphatic leukemia].
Molecular genetic analysis was performed on DNA extracted from blast cells of a patient with acute lymphoblastic leukemia. While k light chain genes were in a germ line configuration rearranged bands were found using both JH and TCR beta probes, thus making a lineage diagnosis uncertain. Northern blot analysis showed that both mu heavy chain and TCR beta chain genes were transcribed in blast cells; TCR beta gene, however, gave a truncated transcript whereas Ig heavy chain gene cluster produced a full length mu mRNA. These data show that the accuracy of lineage diagnosis can be improved if expression studies are performed in association with molecular genetic analysis.
Mariano
M T
MT
Grantini
M
M
Varani
M
M
Mussini
D
D
Sarti
M
M
Colò
A
A
D015342:DNA Probes; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006801:Humans; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D015347:RNA Probes; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D015342:DNA Probes; D015347:RNA Probes; D011948:Receptors, Antigen, T-Cell
Espressione dei geni delle immunoglobuline e del recettore antigenico T-linfocitario in un caso di leucemia linfatica acuta.