title: [Use of isoelectric focusing in studying the composition of allergens from mites].
abstract: The method of the isoelectric separation of allergens isolated from Dermatophagoides ticks with the use of isoelectrofocusing has been improved. 10-12 protein fractions with isoelectric points from 3.50 to 6.55 have been isolated. Allergens obtained from D. farinae and D. pteronyssinus grown on two different nutrient media have been found to contain common protein components. In D. pteronyssinus allergen the presence of a greater number of protein fractions has been noted in the region of pH 5.25-6.00 than in D. farinae allergen.
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Emel'ianova
authors.forename: V M
authors.initials: VM
authors.lastname: Berzhets
authors.forename: L N
authors.initials: LN
authors.lastname: Padiukov
authors.forename: N e
authors.initials: Ne
authors.lastname: Sipitsyna
authors.forename: V A
authors.initials: VA
authors.lastname: Taranov
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D004391:Dust; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D008925:Mites / Q000276:immunology*; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification
chemical_list: D000485:Allergens; D004391:Dust; D011506:Proteins
vernacular_title: Ispol'zovanie izoélektrofokusirovaniia dlia izuchenia sostava allergenov iz kleshcheĭ.