Production of transforming growth factor beta by human peripheral blood monocytes and neutrophils.
Human peripheral blood monocytes and neutrophils secrete TGF-beta. Activation of monocytes with LPS stimulates the secretion of TGF-beta; however, the production of TGF-beta by neutrophils was not altered by treatment with LPS. The secreted TGF-beta appears to be in a fully active form since acid treatment of the conditioned medium does not increase the amount of TGF-beta activity. TGF-beta 1 transcripts were detected at similar levels in both activated and nonactivated monocytes and neutrophils, suggesting that the increase in TGF-beta secretion after activation of monocytes is regulated by a posttranscriptional mechanism. Western blot analysis with anti-N-terminal TGF-beta 1 peptide antibodies indicate the leukocyte-derived TGF-beta is beta 1. In addition, TGF-beta 1 transcripts were detected in rat peritoneal macrophages and in a differentiating human hematopoietic tumor cell line (HEL). The ability of inflammatory cells such as monocytes and neutrophils to produce TGF-beta may play an important role in the function of these cells in wound repair, in the immune response, and in the pathogenesis of fibrotic diseases.
Grotendorst
G R
GR
Department of Biology, University of South Florida, Tampa 33620.
Smale
G
G
Pencev
D
D
D000818:Animals; D000936:Antigen-Antibody Complex / Q000494:pharmacology; D002460:Cell Line; D004731:Endotoxins / Q000494:pharmacology; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D015151:Immunoblotting; D009000:Monocytes / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015291:Transforming Growth Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D000936:Antigen-Antibody Complex; D004731:Endotoxins; D015291:Transforming Growth Factors