title: Inhibition of starfish oocyte maturation by leupeptin analogs, potent trypsin inhibitors.
abstract: Inhibition of subsite-substituted leupeptin analogs, potent trypsin inhibitors, on 1-methyladenine-induced germinal vesicle breakdown was investigated in a starfish, Asterina pectinifera. Of benzyloxycarbonyl(Z)-Leu-P2-argininals, the analog with Ser at P2 residue was the strongest inhibitor, and those with Pro, Leu greater than Thr greater than Gly were followed in this order. In Z-P3-Ser-argininals, ranking of the inhibitory ability was as follows: Phe greater than Leu much greater than Pro greater than Ala at P3 residue. Among 11 analogs synthesized, Z-Phe-Ser-argininal showed the strongest inhibition. The inhibitory potency of the analog was 100-fold stronger than that of leupeptin (acetyl-Leu-Leu-argininal). Thus, trypsin-like enzyme possessing a narrow subsite specificity participates in oocyte maturation in the starfish.
authors.affiliation: Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Japan.
authors.forename: M T
authors.initials: MT
authors.lastname: Sawada
authors.forename: T
authors.initials: T
authors.lastname: Someno
authors.forename: M
authors.initials: M
authors.lastname: Hoshi
authors.forename: H
authors.initials: H
authors.lastname: Sawada
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D007976:Leupeptins / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D013215:Starfish; D013329:Structure-Activity Relationship; D014361:Trypsin Inhibitors / Q000494:pharmacology*
chemical_list: D007976:Leupeptins; D009842:Oligopeptides; D014361:Trypsin Inhibitors; C008407:1-methyladenine; D000225:Adenine