Experimental model for metastasis of intraocular melanoma: preventive role of natural killer cells.
A murine melanoma cell line, B16-BL6, was inoculated in various body sites of the mouse, including the anterior chamber of the eye, and an analysis was carried out of the relationship between the anatomical site of tumor inoculation and the tumor growth or the pattern of tumor metastasis. It was found that not only the primary tumor growth but also the metastatic pattern was greatly affected by the site of the primary tumor. Fifteen days after the tumor transplantation, the mean weight of the primary tumors transplanted in the ear or in the abdominal flank became five to ten times larger than those transplanted in the eye or in the footpad. Furthermore, intraocular melanomas rapidly developed lung metastasis, whereas subcutaneous melanoma primarily developed lymph node metastasis. It was also found that natural killer cells had a preventive role in the early phase of hematogenous dissemination from the intraocular melanoma.
Yokoyama
T
T
Department of Ophthalmology, Tohoku University School of Medicine, Japan.
Yoshie
O
O
Mizuno
K
K
D000818:Animals; D000867:Anterior Chamber; D002461:Cell Line, Transformed; D015353:Eye Enucleation; D005134:Eye Neoplasms; D007370:Interferon Type I / Q000627:therapeutic use; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology / Q000556:secondary* / Q000628:therapy; D008207:Lymphatic Metastasis; D008297:Male; D008545:Melanoma / Q000276:immunology / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins; D012983:Soft Tissue Neoplasms / Q000276:immunology / Q000556:secondary; D013848:Thigh; D013997:Time Factors
D007370:Interferon Type I; D011994:Recombinant Proteins