Rabbit brown adipose tissue uncoupling protein MRNA: use of only one of two polyadenylation signals in its processing.
A cDNA containing the complete coding sequence of rabbit brown adipose tissue uncoupling protein was isolated and sequenced. The coding region is 80.6% identical to rat UCP cDNA and the protein is about 86% identical to the rat and hamster proteins. Despite the presence of 2 AATAAA polyadenylation consensus sequences in rabbit UCP cDNA, only one rabbit UCP mRNA was detected indicating that only the 3'-downstream signal is used in contrast to rat and mouse where both are used.
Balogh
A G
AG
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Ridley
R G
RG
Patel
H V
HV
Freeman
K B
KB
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D014475:Uncoupling Agents / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1; D011061:Poly A