[A study of the cloned human erythropoietin gene expression in mammalian cells in vitro].
The human gene coding for the principal factor of erythroid cells differentiation, erythropoietin, has been isolated from the genomic phage library using an oligonucleotide probe for the gene. The construction of series of plasmids carrying the erythropoietin gene under the control of various regulatory elements is reported. Efficiency of the erythropoietin gene expression was estimated by testing of the biologically active erythropoietin in conditioned media 48 h after transient transfection of COS 1 and CHO cell lines.
Kramerov
I A
IA
Zelenin
M G
MG
Filiakina
N S
NS
Kolobkov
S L
SL
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D004921:Erythropoietin / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D014162:Transfection
D004921:Erythropoietin; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII
Izuchenie ékspressii klonirovannogo gena éritropŏétina cheloveka v kletkakh mlekopitaiushchikh in vitro.