Anti-kidney antibody teratogenesis: evidence for a direct action on the rat conceptus by a mechanism involving the yolk sac.
IgG from anti-rat kidney antiserum (anti-K) was added to 48-h cultures of 9.5-day whole rat conceptuses. At concentrations of 25 micrograms/ml and above, dysmorphogenesis of embryos (optic vesicle defects) was observed. Significant embryonic growth retardation was apparent at 75 micrograms/ml and above. Other abnormalities (neural tube defects, deficits in axial rotation, reduced somite numbers) were seen at 100 and 150 micrograms/ml. In separate experiments, anti-K inhibited pinocytosis of 125I-polyvinylpyrrolidone by isolated yolk sacs. All of these effects of anti-K are similar to those produced by anti-visceral yolk sac antiserum suggesting a similar mechanism of teratogenic action, namely interference with yolk-sac-mediated embryotrophic nutrition.
Freeman
S J
SJ
Smith Kline & French Research Ltd., Welwyn, Herts, U.K.
Marlow
R
R
Leung
C C
CC
D000015:Abnormalities, Multiple / Q000209:etiology*; D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects*; D007106:Immune Sera / Q000633:toxicity*; D007457:Iodine Radioisotopes; D007668:Kidney / Q000276:immunology*; D009924:Organ Culture Techniques; D010873:Pinocytosis / Q000187:drug effects; D011205:Povidone / Q000493:pharmacokinetics; D051381:Rats; D013723:Teratogens; D015017:Yolk Sac / Q000187:drug effects*
D007106:Immune Sera; D007457:Iodine Radioisotopes; D013723:Teratogens; D011205:Povidone