title: Comparison of HeLa 229 and McCoy cell cultures for detection of Chlamydia trachomatis in clinical specimens.
abstract: Consecutive clinical specimens of Chlamydia trachomatis (1,048) were inoculated in parallel on DEAE-dextran- and cycloheximide-treated HeLa 229 cells and cycloheximide-treated McCoy cells. HeLa 229 cell culture detected 113 positive specimens, and McCoy cell culture detected 103 positive specimens. This difference is not significant. However, HeLa 229 cell culture yielded significantly more inclusions than McCoy cell culture in the 95 specimens positive in both cell types (P = 0.042). For routine diagnostic purposes, a choice for one of the cell types may be determined by local preferences.
authors.affiliation: Department of Clinical Microbiology, University Hospital Rotterdam, The Netherlands.
authors.forename: E A
authors.initials: EA
authors.lastname: Thewessen
authors.forename: I
authors.initials: I
authors.lastname: Freundt
authors.forename: J H
authors.initials: JH
authors.lastname: van Rijsoort-Vos
authors.forename: E
authors.initials: E
authors.lastname: Stolz
authors.forename: M F
authors.initials: MF
authors.lastname: Michel
authors.forename: J H
authors.initials: JH
authors.lastname: Wagenvoort
mesh_terms: D002460:Cell Line; D002584:Cervix Uteri / Q000382:microbiology*; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D003470:Culture Media; D003513:Cycloheximide; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D014521:Urethra / Q000382:microbiology*; D014526:Urethritis / Q000175:diagnosis / Q000382:microbiology; D002575:Uterine Cervicitis / Q000175:diagnosis / Q000382:microbiology
chemical_list: D003470:Culture Media; D003513:Cycloheximide