Report of workshop on cellular protein databases derived from two-dimensional polyacrylamide gel electrophoresis.
A workshop entitled Cellular Protein Databases from Two-Dimensional Gel Electrophoresis was held in Atlanta, Georgia, 28 February-1 March 1987. Its purpose was to assess the status of two-dimensional gel electrophoresis of proteins as a research methodology in biological systems, particularly in the generation of cellular protein databases. The workshop participants summarized current studies on a variety of biological systems, both prokaryotic and eukaryotic. Analysis of the progress being made led to the conclusion that electrophoretic techniques, supported by automatic scanning of gel images and computer-assisted processing, analysis and matching of gel images, are now capable of generating databases of great potential value. Factors affecting the reproducibility of protein spot patterns on gels were identified, and the extent to which gel pattern variability causes difficulties in communicating results and in integrating information from different laboratories was assessed. Measures were suggested to help overcome obstacles to the generation of comprehensive cellular protein databases from the electrophoretic resolution of total cellular proteins.
Neidhardt
F C
FC
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor.
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007256:Information Systems; D011506:Proteins / Q000032:analysis*
D011506:Proteins