Measurement of urinary somatomedin C by a chemiluminescent method.
We present a method for the measurement of the total Somatomedin C (SmC) content in human early morning urine samples after dialysis, extraction, and concentration. We modified a chemiluminescence immunoassay, previously developed for SmC determination in serum, for analysis of SmC in urine. Appropriate sensitivity was obtained by the preparation of a new chemiluminescent tracer (AEEI-COOH-SmC) and the optimization of a competitive non-equilibrium immunoassay system which had a detection limit of 0.24 fmol SmC per tube.
Orlando
C
C
Department of Clinical Physiopathology University of Florence, Italy.
Tode
B
B
Pinzani
P
P
Bassi
F
F
Messeri
G
G
D002352:Carrier Proteins / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000652:urine*; D008163:Luminescent Measurements; D011863:Radioimmunoassay; D013002:Somatomedins / Q000652:urine*
D002352:Carrier Proteins; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I