Development and application of immunoassay for paraquat: radioimmunoassay.
A sensitive radioimmunoassay for paraquat is reported. Anti-paraquat antisera were produced by repeated immunization in rabbits with 1-methyl, 1'-hexanoic acid-4,4'-bipyridinium (MHBP) coupled to bovine serum albumin (BSA). Less than 0.5 ng of paraquat dichloride was detectable by this assay system. These antisera were strongly cross-reactive with the bipyridyl ring and methyl group in either the 1- or 1'-position of paraquat. The determination of paraquat in tissues of paraquat-poisoned cadavers was also carried out.
Nagao
M
M
Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Takatori
T
T
Terazawa
K
K
Wu
B
B
Wakasugi
C
C
Masui
M
M
Ikeda
H
H
D001344:Autopsy; D003124:Colorimetry; D003429:Cross Reactions; D006801:Humans; D010269:Paraquat / Q000032:analysis* / Q000276:immunology / Q000506:poisoning; D011863:Radioimmunoassay; D014018:Tissue Distribution
D010269:Paraquat