[Modification of the streptokinase functional groups during iodination].
Modification of tyrosine residues was detected in streptokinase molecule during iodination. A nonlinear type of the modification reaction allowed to suppose that tyrosine residues were heterogenous. As shown by gel chromatography the modified streptokinase did not develop stable complexes with human plasminogen even at low ratios of iodine/streptokinase, however the activating effects of the streptokinase were maintained. During iodination the secondary structure of streptokinase appears to be altered, the level of which depended on the molar ratio iodine/streptokinase.
Nikandrov
V N
VN
Kaziuchits
O A
OA
D006801:Humans; D007455:Iodine / Q000494:pharmacology*; D007700:Kinetics; D010958:Plasminogen / Q000378:metabolism; D011487:Protein Conformation; D013300:Streptokinase / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D014443:Tyrosine; D010958:Plasminogen; D007455:Iodine; D013300:Streptokinase
Modifikatsiia funktsional'nyukh grupp streptokinazy pri ĭodirovanii.