Species-specific shrimp allergens: RAST and RAST-inhibition studies.
Two edible shrimp species are widely available in Louisiana, Penaeus setifecus (white shrimp) and Penaeus aztecus (brown shrimp). Some sensitive individuals report only occasional allergic symptoms after shrimp ingestion, suggesting that there may be species-specific allergens. To investigate this possibility, we evaluated shrimp species-specific reactivity in 31 individuals with a history of immediate hypersensitivity reactions after shrimp ingestion with skin prick tests and RASTs with white and brown shrimp extracts. On selected individuals, RAST-inhibition studies were performed with white shrimp and/or brown shrimp-coupled disks, with white and/or brown shrimp extracts as inhibiting allergen. Positive skin tests to both types of extract were observed in 77% (23/30) of the subjects; one individual reacted to brown shrimp extract only. Elevated RASTs to both extracts were observed in 16/31 study participants; one subject reacted only to white shrimp extracts and two subjects to brown shrimp extract alone. Sera from two individuals tested by RAST inhibition recognized qualitatively different allergens in brown and white shrimp extracts, supporting the hypothesis that there are species-specific shrimp allergens. Species specificity is important because it may explain the intermittent symptoms of some study subjects. The percentage of shrimp-sensitive subjects testing positive by skin test and RAST can be increased by use of extracts from more than one species of shrimp.
Morgan
J E
JE
Department of Medicine, Tulane University Medical Center, New Orleans, La.
O'Neil
C E
CE
Daul
C B
CB
Lehrer
S B
SB
D000328:Adult; D000485:Allergens; D000818:Animals; D001667:Binding, Competitive; D033364:Decapoda / Q000276:immunology*; D005260:Female; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D008145:Louisiana; D008297:Male; D008875:Middle Aged; D011852:Radioallergosorbent Test; D013045:Species Specificity
D000485:Allergens; D007073:Immunoglobulin E