title: Mismatch repair involving localized DNA synthesis in extracts of Xenopus eggs.
abstract: Repair of heteroduplex DNA containing G.T or A.C mismatches or containing two tandem unpaired bases occurred in vitro with Xenopus egg extracts as detected by a physical assay. The repair was accompanied by a mismatch-stimulated and mismatch-localized DNA synthesis. Repaired molecules, separated from unrepaired molecules, showed a 20- to 100-fold increase in DNA synthesis in the region of the mismatch compared to regions distant from the mismatch. The remaining unrepaired heteroduplex DNA included molecules that also displayed mismatch-stimulated DNA synthesis in the mismatch-proximal regions. These may represent intermediates in the repair process. The patterns of DNA synthesis suggest that repair begins at some distance from the mismatch and that as much as 1 kilobase or more can be involved in the mismatch-stimulated synthesis.
authors.affiliation: Institut Jacques Monod. Centre National de la Recherche Scientifique, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Brooks
authors.forename: C
authors.initials: C
authors.lastname: Dohet
authors.forename: G
authors.initials: G
authors.lastname: Almouzni
authors.forename: M
authors.initials: M
authors.lastname: Méchali
authors.forename: M
authors.initials: M
authors.lastname: Radman
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D004260:DNA Repair; D004261:DNA Replication; D005260:Female; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics / Q000302:isolation & purification; D009865:Oocytes / Q000378:metabolism*; D015183:Restriction Mapping; D014982:Xenopus laevis
chemical_list: D009692:Nucleic Acid Heteroduplexes; D004247:DNA