Isolation and structural studies of phosphate-containing oligosaccharides from alkaline and acid hydrolysates of Streptococcus pneumoniae type 6B capsular polysaccharide.
The capsular polysaccharide of Streptococcus pneumoniae serotype 6B [----2)-alpha-D-Galp-(1----3)-alpha-D-Glcp-(1----3)-alpha-L-Rhap-( 1----4)- D-RibOH-(5-P----]n was depolymerised under alkaline (NaOH) and acidic (HF) conditions. The former treatment yielded, as the major component, alpha-2-P-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-5- P-RibOH. The latter treatment at -16 degrees gave alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH-(5-P----2)- alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH and at 4 degrees gave alpha-Galp-(1----3)-alpha-Glcp-(1----3)-alpha-Rhap-(1----4)-Rib OH. These oligosaccharides were characterised by sugar analysis, f.a.b.-m.s., and 1H- and 13C-n.m.r. spectroscopy.
van Dam
J E
JE
Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Breg
J
J
Komen
R
R
Kamerling
J P
JP
Vliegenthart
J F
JF
D000143:Acids; D000468:Alkalies; D002241:Carbohydrates / Q000032:analysis; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000032:analysis / Q000302:isolation & purification*; D010710:Phosphates / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013296:Streptococcus pneumoniae
D000143:Acids; D000468:Alkalies; D002241:Carbohydrates; D009844:Oligosaccharides; D010710:Phosphates; D011135:Polysaccharides, Bacterial