title: Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD.
abstract: MyoD is a master regulatory gene for myogenesis. Under the control of a retroviral long terminal repeat, MyoD was expressed in a variety of differentiated cell types by using either a DNA transfection vector or a retrovirus. Expression of muscle-specific proteins was observed in chicken, human, and rat primary fibroblasts and in differentiated melanoma, neuroblastoma, liver, and adipocyte lines. The ability of MyoD to activate muscle genes in a variety of differentiated cell lines suggests that no additional tissue-specific factors other than MyoD are needed to activate the downstream program for terminal muscle differentiation or that, if such factors exist, they are themselves activated by MyoD expression.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
authors.forename: H
authors.initials: H
authors.lastname: Weintraub
authors.forename: S J
authors.initials: SJ
authors.lastname: Tapscott
authors.forename: R L
authors.initials: RL
authors.lastname: Davis
authors.forename: M J
authors.initials: MJ
authors.lastname: Thayer
authors.forename: M A
authors.initials: MA
authors.lastname: Adam
authors.forename: A B
authors.initials: AB
authors.lastname: Lassar
authors.forename: A D
authors.initials: AD
authors.lastname: Miller
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D005822:Genetic Vectors; D006801:Humans; D008099:Liver; D008545:Melanoma; D008980:Moloney murine sarcoma virus / Q000235:genetics; D009132:Muscles / Q000166:cytology*; D009447:Neuroblastoma; D014162:Transfection