Decrease in CuZn-superoxide dismutase mRNA level during differentiation of human monocytic and promyelotic leukemia cells.
Change in the level of CuZn-superoxide dismutase (SOD) mRNA was examined using a molecular probe during differentiation of human monocytic leukemia U937 cells or promyelotic leukemia HL-60 cells induced by either 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or dimethylsulfoxide (DMSO). CuZn-SOD mRNA levels were found to decrease during the course of differentiation, and this response is specific for differentiation, since the treatment of human B cell leukemia cells or normal diploid fibroblasts with TPA failed to have any effect on the level of CuZn-SOD mRNA. The activity of CuZn-SOD in U937 cells also decreased during differentiation, but following that of the CuZn-SOD mRNA level. The expression of the CuZn-SOD gene is thus concluded to diminish during the differentiation of HL-60 and U937 cells.
Saito
H
H
Department of Cancer Cell Research, University of Tokyo, Japan.
Kuroki
T
T
Nose
K
K
D015152:Blotting, Northern; D002454:Cell Differentiation; D003300:Copper; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005347:Fibroblasts; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015032:Zinc
D012333:RNA, Messenger; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide