Biochemical examination on the increased superoxide dismutase-like plasma substance observed in a state of acute gastric mucosal lesions.
When experimental acute gastric mucosal lesions were produced in guinea pig by water-immersion and restraint stress, superoxide dismutase (SOD)-like substance in the plasma increased. On analysis by gel filtration, it was shown that the molecular weight of the increased SOD-like plasma substance was about 130,000, and even after treatment with trypsin, 84% of this substance remained. Since the molecular weight of intracellular SOD is about 40,000, it seems that this substance is similar to extracellular SOD, located on the endothelial cell-surface, as previously reported by Marklund et al. Our results suggest that in the presence of acute gastric mucosal lesions, SOD-like plasma substance is not identical to intracellular SOD, which derived from cell destruction by stress or free radical-induced microvascular damage or by hemolysis. Furthermore, this substance may itself work as a scavenger of free radicals generated under conditions, such as these described in the present experiment.
Ohara
T
T
Third Department of Internal Medicine, School of Medicine, Tohoku University, Sendai, Japan.
Matsuda
K
K
Shibuya
D
D
Eda
Y
Y
Yuki
T
T
Asaki
S
S
Toyota
T
T
Kurokawa
T
T
Sasaki
R
R
D000818:Animals; D002850:Chromatography, Gel; D005753:Gastric Mucosa; D006168:Guinea Pigs; D007101:Immersion; D008297:Male; D008970:Molecular Weight; D012149:Restraint, Physical; D013276:Stomach Ulcer / Q000201:enzymology* / Q000209:etiology; D013312:Stress, Physiological / Q000150:complications / Q000201:enzymology; D013482:Superoxide Dismutase / Q000097:blood*; D014357:Trypsin / Q000494:pharmacology
D013482:Superoxide Dismutase; D014357:Trypsin