Actin stress fiber content of regenerated endothelial cells correlates with intramural retention of intermediate plus low density lipoproteins in rat aorta after balloon injury.
The rat aortic model of endothelial injury (balloon catheter induced) has been used to establish whether changes in protein intramural penetration in specific areas of the injured aorta were accompanied by phenotypic modifications of the regenerated endothelial cells covering these particular regions. Iodinated lipoproteins (IDL/LDL fraction) and albumin were used as tracers to localize protein permeability and retention in the aorta. Lipoproteins, but not albumin, were retained in the thickened areas covered with regenerated endothelium (i.e., 60 days after balloon induced injury). Neither lipoproteins nor albumin were retained in the other aortic areas studied, including the intimal thickening of de-endothelialized areas (15 days after injury). The relative volume of cytoplasmic stress fibers was significantly increased in regenerated endothelium covering thickened areas as compared with the other regions of the injured or normal aorta. The accumulation of lipids usually observed in atherosclerotic lesions, compatible with the trapping of lipoproteins by the matrix component of the intimal thickening, may be related to modulated features of endothelial cells regenerated over thickened areas of the aorta.
Barja
F
F
Division of Diabetology, Hôpital Cantonal Universitaire, Geneva, Switzerland.
Blatter
M C
MC
James
R W
RW
Pometta
D
D
Gabbiani
G
G
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000293:injuries / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008077:Lipoproteins, LDL