title: Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody.
abstract: Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (VK, JK, VH, JH) as do anti-azophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of beta-octylglucoside as tetragonal bipyramids in the space group of either P4(1)2(1)2 or P4(3)3(1)2 and with unit cell dimensions of a = b = 77.9 A, and c = 146.7 A. They diffract X-rays to better than 2.7 A resolution. Data up to 2.7 A resolution have been collected.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: S F
authors.initials: SF
authors.lastname: Lin
authors.forename: M
authors.initials: M
authors.lastname: Coll
authors.forename: M L
authors.initials: ML
authors.lastname: Gefter
authors.forename: M N
authors.initials: MN
authors.lastname: Margolies
authors.forename: A H
authors.initials: AH
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001391:Azo Compounds / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments; D007135:Immunoglobulin Variable Region; D051379:Mice; D008815:Mice, Inbred Strains; D014961:X-Ray Diffraction; D010132:p-Azobenzenearsonate / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D001391:Azo Compounds; D007140:Immunoglobulin Fab Fragments; D007135:Immunoglobulin Variable Region; D010132:p-Azobenzenearsonate