title: Clonal allele loss in gastrointestinal cancers.
abstract: Using a panel of DNA probes for hypervariable DNA regions we screened 52 gastrointestinal carcinomas for clonal allele losses on chromosomes 1, 5, 7, 12, 16 and 17. A total of 24/35 informative cases of colorectal cancers showed loss of constitutional heterozygosity at a locus on chromosome 17p, while 9/31 cases informative for a locus on 5q showed allele loss. Loss of sequences at 5q was linked to allele loss at 17p with a single exception. In gastric cancers loss of heterozygosity most frequently occurred at 1q (5/10 tumours) and at 12q (6/11 tumours). Gastrointestinal tumours show consistent chromosomal losses and the loci involved are different in gastric and colorectal cancers.
authors.affiliation: Department of Haematology, John Radcliffe Hospital, Headington, Oxford, UK.
authors.forename: M F
authors.initials: MF
authors.lastname: Fey
authors.forename: C
authors.initials: C
authors.lastname: Hesketh
authors.forename: J S
authors.initials: JS
authors.lastname: Wainscoat
authors.forename: S
authors.initials: S
authors.lastname: Gendler
authors.forename: S L
authors.initials: SL
authors.lastname: Thein
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000483:Alleles; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D015179:Colorectal Neoplasms / Q000235:genetics*; D015342:DNA Probes; D006579:Heterozygote; D006801:Humans; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D015342:DNA Probes