Comparison of microscale ultracentrifugation and equilibrium dialysis for the determination of the unbound fraction of theophylline in human serum.
A microscale ultracentrifugation procedure for the measurement of free theophylline in serum is described and evaluated by comparison with an equilibrium dialysis method. Provided an ultracentrifuge is available, micro-ultracentrifugation is a simple, cost-effective technique for the routine determination of free drug fractions.
McKenzie
K
K
Department of Biochemistry, Royal Infirmary, Glasgow, UK.
Watson
I D
ID
Stewart
M J
MJ
D003365:Costs and Cost Analysis; D003956:Dialysis / Q000379:methods; D006801:Humans; D011485:Protein Binding; D013806:Theophylline / Q000097:blood*; D014461:Ultracentrifugation / Q000379:methods
D013806:Theophylline