Crystallization and preliminary X-ray diffraction studies of oxidized flavodoxin from Chondrus crispus, a red alga.
Crystals of the oxidized form of flavodoxin from a red alga, Chondrus crispus, have been grown in ammonium sulfate solution by the dialysis method. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 63.6, b = 48.8, and c = 56.8 A. The asymmetric unit contains one molecule of flavodoxin. The crystals diffract X-rays to about 2.0 A resolution and are stable to X-ray beams. The diffraction patterns changed significantly upon soaking the crystal in a solution of a platinum complex. The major heavy-atom sites in the platinum derivative crystal have been identified from the difference Patterson function calculated at 4 A resolution.
Fukuyama
K
K
Department of Biology, Faculty of Science, Osaka University.
Matsubara
H
H
Katsube
Y
Y
Rogers
L J
LJ
D003460:Crystallization; D005418:Flavodoxin / Q000032:analysis*; D005420:Flavoproteins / Q000032:analysis*; D010084:Oxidation-Reduction; D000461:Rhodophyta / Q000032:analysis*; D014961:X-Ray Diffraction
D005418:Flavodoxin; D005420:Flavoproteins