[Synthesis of peptides, catalyzed by ficin].
Synthesis of a model peptide Z-Ala-Val-Gly-OH catalyzed by ficin in phosphate buffer was studied with water-soluble esters Z-Ala-OCH2CO-Gly-OH and Z-Ala-OCH2CO-NHC2H4SO3Na as carboxyl components. The maximal yield of the peptide was obtained at pH 8-9. The effect of temperature and concentration of reagents was studied in the model reaction.
Schuster
J
J
Mitin
Iu V
IuV
Jakubke
H D
HD
D002384:Catalysis; D003546:Cysteine Endopeptidases; D005361:Ficain; D007202:Indicators and Reagents; D010455:Peptides / Q000138:chemical synthesis*; D013696:Temperature
D007202:Indicators and Reagents; D010455:Peptides; D003546:Cysteine Endopeptidases; D005361:Ficain
Sintez peptidov, kataliziruemyĭ fitsinom.