[The interaction of macrophages and hematopoietic stem cells of mice in vitro and in vivo].
In the previous in vivo experiments it was shown that macrophages could modify the direction of haematopoietic stem cell (CFU-S) differentiation from erythroid to myeloid one, when injected simultaneously to lethally irradiated mice. Stable absorption of 50% CFU-S on the macrophage monolayer was documented in in vitro experiments, when it was incubated for 1 hour together with bone marrow cells. After administration to syngeneic recipients the CFU-S adsorbed by macrophages formed myeloid spleen colonies mainly. The nonadsorbed part of CFU-S underwent similar changes in the direction of differentiation, but only after a longer (2.5 hour) coincubation. The involvement of monokines in the control of the early differentiation of haematopoietic stem cells is suggested.
Kravtsov
V D
VD
Lasunskaia
E B
EB
Freĭdlin
I S
IS
D000327:Adsorption; D000818:Animals; D001854:Bone Marrow Cells; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D066298:In Vitro Techniques; D008264:Macrophages / Q000166:cytology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010529:Peritoneal Cavity / Q000166:cytology; D013154:Spleen / Q000166:cytology
Vzaimodeĭstvie makrofagov i stvolovykh krovetvornykh kletok mysheĭ in vitro i in vivo.