title: Binding of saccharides to ricin E isolated from small castor beans.
abstract: The binding of saccharides to ricin E isolated from small castor beans was studied by equilibrium dialysis and spectroscopy. Equilibrium dialysis data indicate that ricin E has two galactose-binding sites, a high affinity site (HA-site) and a low affinity site (LA-site). The binding of specific saccharides to ricin E induces a shift of the fluorescence spectrum to shorter wavelength by 3 nm and UV-difference spectra with a maximum at 290 nm and a negative intensity around 300 nm. The interaction of ricin E with its specific saccharides was analyzed in terms of the variation of the intensity at 320 nm in the fluorescence spectrum and the magnitude of the negative intensity at 300 nm in the UV-difference spectra as functions of saccharide concentration. The results indicate that these spectroscopic changes are representative of the binding of saccharides to the LA-site, which contains a tryptophan residue. By comparing the association constants of saccharides for ricin E with those for ricin D, isolated from the large castor beans, it was found that the HA of ricin E binds saccharides with an affinity of less than one-half that of ricin D, while the saccharide-binding abilities of the LA-site of the two ricins were about the same.
authors.affiliation: Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka.
authors.forename: T
authors.initials: T
authors.lastname: Hatakeyama
authors.forename: H
authors.initials: H
authors.lastname: Ohba
authors.forename: N
authors.initials: N
authors.lastname: Yamasaki
authors.forename: G
authors.initials: G
authors.lastname: Funatsu
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002367:Ricinus communis / Q000032:analysis*; D003956:Dialysis; D006863:Hydrogen-Ion Concentration; D007785:Lactose / Q000032:analysis; D037121:Plant Lectins; D010947:Plants, Toxic; D012276:Ricin / Q000032:analysis*; D012278:Ricinus / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002241:Carbohydrates; D037121:Plant Lectins; D012276:Ricin; D007785:Lactose