The use of monoclonal antibodies to define levels of cystatin C in normal human serum.
We established four hybridoma cell lines which secreted monoclonal antibodies against human cystatin C. These monoclonal antibodies were of IgA(kappa), IgG2a(lambda), IgG1(kappa), and IgG1(lambda) isotypes, respectively. The association constants (Ka) of the three IgG monoclonal antibodies ranged from 3.6 x 10(9) M-1 to 7.3 x 10(10) M-1. An ELISA technique for the measurement of cystatin C was established by using one of these monoclonal antibodies. The assay procedure was highly specific, sensitive, and reproducible. The lower detectable limit of cystatin C by this procedure was 1.9 ng/ml. The level of cystatin C in normal human serum was 1.16 +/- 0.91 micrograms/ml (mean +/- S.D., n = 274).
Ishiguro
H
H
Department of Biochemistry, Nagoya City University Medical School, Japan.
Ohkubo
I
I
Mizokami
M
M
Titani
K
K
Sasaki
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D055316:Cystatin C; D015891:Cystatins; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D011480:Protease Inhibitors / Q000097:blood / Q000276:immunology; D011506:Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; C525030:CST3 protein, human; C525031:Cst3 protein, mouse; D055316:Cystatin C; D015891:Cystatins; D011480:Protease Inhibitors; D011506:Proteins