Effective monitoring of residues of nortestosterone and its major metabolite in bovine urine and bile.
The results of a newly developed method for the detection and identification of residues of nortestosterone (NT) and one of its major metabolites, 17 alpha-nortestosterone (epiNT) are described. The method is based on sample clean-up by immunoaffinity chromatography and detection by high-performance liquid chromatography and/or gas chromatography-mass spectrometry (selected-ion monitoring). All samples of bile from calves that had been treated with NT contained significant amounts of epiNT (6-18 micrograms/l). The NT content of these samples, if detectable, was below 1 microgram/l. Urine contained, with one exception, less than 1 microgram/l epiNT. NT itself if detectable, was, present in urine or bile at levels below 0.1 microgram/l. The results corresponds well with results obtained with a radioimmunoassay procedure.
van Ginkel
L A
LA
National Institute of Public Health and Environmental Protection, Laboratory for Residue Analysis, Bilthoven, The Netherlands.
Stephany
R W
RW
van Rossum
H J
HJ
van Blitterswijk
H
H
Zoontjes
P W
PW
Hooijschuur
R C
RC
Zuydendorp
J
J
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D007106:Immune Sera; D007074:Immunoglobulin G / Q000032:analysis; D009277:Nandrolone / Q000378:metabolism* / Q000652:urine; D011817:Rabbits; D011863:Radioimmunoassay; D013056:Spectrophotometry, Ultraviolet
D007106:Immune Sera; D007074:Immunoglobulin G; D009277:Nandrolone