title: Relative specificities of inhibition of acid cholesteryl ester hydrolase and neutral cholesteryl ester hydrolase in cultured rabbit aortic smooth muscle cells by esterastin and cholesteryl oleyl ether.
abstract: Rabbit aortic smooth muscle cells in culture were incubated with 0.04-500 M esterastin. Acid cholesteryl ester hydrolase (ACEH) and neutral cholesteryl ester hydrolase (NCEH) activities were inhibited to a comparable degree, with 50% inhibition occurring in the range of 0.4 M esterastin. Cells incubated with cholesteryl oleyl ether showed 50% inhibition of NCEH at 5.0 M, but no inhibition of ACEH over a concentration range of 0.2-20 M. This relative specificity of cholesteryl oleyl ether for NCEH can be employed to study the relative roles of ACEH vs. NCEH in preventing cellular cholesteryl ester accumulation.
authors.affiliation: Department of Pathology, Harbor-UCLA Medical Center, Torrance 90509.
authors.forename: R J
authors.initials: RJ
authors.lastname: Morin
authors.forename: S K
authors.initials: SK
authors.lastname: Peng
mesh_terms: D000818:Animals; D001011:Aorta; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007783:Lactones / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D011817:Rabbits; D002787:Sterol Esterase / Q000037:antagonists & inhibitors*
chemical_list: D007783:Lactones; C023511:cholesteryl oleyl ether; C017293:esterastin; D002784:Cholesterol; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase