Studies on ferritin in rat liver and spleen during repeated phlebotomy.
1. The ferritin content of liver and spleen in normal and iron-loaded rats decreased during repeated phlebotomy. 2. During increased iron demand, ferritin is degraded in toto. 3. With the ESI and EELS technique the iron distribution was followed in different cell types and cellular compartments. 4. We have demonstrated two methods of iron mobilisation: (a) catabolism of lysosomal ferritin in toto and (b) delivery of ferritin from parenchymal cell into the bile and degradation of ferritin in toto.
Mostert
L J
LJ
Institute of Chemical Pathology, Erasmus University Rotterdam, The Netherlands.
Cleton
M I
MI
de Bruijn
W C
WC
Koster
J F
JF
van Eijk
H G
HG
D000818:Animals; D001815:Bloodletting; D002451:Cell Compartmentation; D002454:Cell Differentiation; D005293:Ferritins / Q000378:metabolism*; D007505:Iron-Dextran Complex / Q000494:pharmacology; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008247:Lysosomes / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000378:metabolism* / Q000648:ultrastructure
D007505:Iron-Dextran Complex; D005293:Ferritins