title: Quantitation of IgG antibody to Streptococcus pneumoniae vaccine by ELISA and FAST-ELISA using tyraminated antigen.
abstract: We examined two ELISA methods for measuring antibodies to Streptococcus pneumoniae using tyraminated S. pneumoniae polysaccharide types 3, 7N, 9F and 14 as antigens. The ELISA has the usual format with a relatively long incubation time whereas the FAST-ELISA has a short incubation time and employs a different solid-phase configuration. We showed that both techniques can be used for the detection of antibodies to S. pneumoniae polysaccharides. Although its analytical sensitivity is about 1/10 of that of the ELISA, the FAST-ELISA is sufficiently sensitive to distinguish protective from unprotective levels of antibodies to the types of S. pneumoniae studied. In studying pre- and post-immunization response, we showed that type 3 is the most immunogenic.
authors.affiliation: Specialty Laboratories, Inc., Santa Monica, CA 90404.
authors.forename: F N
authors.initials: FN
authors.lastname: Boctor
authors.forename: N E
authors.initials: NE
authors.lastname: Barka
authors.forename: M S
authors.initials: MS
authors.lastname: Agopian
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D014439:Tyramine
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial; D014439:Tyramine