Single cell analysis of DNA modifications induced by chemical carcinogens and cytostatic drugs.
Antibodies recognizing specific DNA modifications allow the immunocytochemical visualization and quantification of these modifications at the level of the individual cell. Thus, the formation and repair of DNA adducts induced by chemical mutagens and carcinogens and by cytostatic drugs can be studied in very small samples in relation to e.g. cell type and tissue localization. A number of existing and potential applications in the fields of chemical carcinogenesis, chemical mutagenesis, experimental chemotherapy and molecular epidemiology are illustrated.
Den Engelse
L
L
Bax
J
J
Terheggen
P M
PM
Van Schooten
F J
FJ
Van Benthem
J
J
Wild
C P
CP
Scherer
E
E
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002273:Carcinogens; D002478:Cells, Cultured; D004249:DNA Damage; D006801:Humans; D051381:Rats
D000970:Antineoplastic Agents; D002273:Carcinogens