title: Cytosolic ionized calcium concentration in isolated chondrocytes from each zone of the growth plate.
abstract: In isolated chondrocytes from the growth plate, there is an increase in both the cytosolic ionized calcium concentration and in total cell calcium content as the cells approach the mineralization front. The reserve zone chondrocytes have a cytosolic ionized calcium concentration, [Ca2+]i, of 124 +/- 23 nM and a total cell calcium content, CaT, of 12.8 +/- 6.8 nmol/mg cell protein. Proliferative zone chondrocytes have a [Ca2+]i of 172 +/- 29 nM and a CaT of 16.1 +/- 11.8 nmol/mg cell protein. Hypertrophic zone chondrocytes have a [Ca2+]i of 273 +/- 49 nM and a CaT of 35.8 +/- 16 nmol/mg protein. Chondrocytes isolated from the entire growth plate have a [Ca2+]i of 132 +/- 29 nM and a CaT of 13.2 +/- 2.5 nmol/mg cell protein. Chondrocytes isolated from noncalcifying hyaline cartilage have a [Ca2+]i of 85 +/- 10 nM and a CaT of 11.5 +/- 1.4 nmol/mg cell protein. There appears to be a correlation between intracellular calcium accumulation, an increase in the intracellular ionized calcium concentration, and the process of cartilage matrix mineralization.
authors.affiliation: McKay Laboratory of Orthopaedic Research, University of Pennsylvania School of Medicine, Philadelphia 19104.
authors.forename: J P
authors.initials: JP
authors.lastname: Iannotti
authors.forename: C T
authors.initials: CT
authors.lastname: Brighton
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism*; D002417:Cattle; D002469:Cell Separation; D002470:Cell Survival; D003600:Cytosol / Q000378:metabolism*; D006132:Growth Plate / Q000378:metabolism*; D007477:Ions; D009994:Osmolar Concentration; D012146:Rest
chemical_list: D007477:Ions; D002118:Calcium