Modification by indomethacin of airway contractile responses in normal and sensitized guinea-pigs.
Active sensitization of guinea-pigs resulted in an increase in responsiveness and sensitivity of tracheal and lung parenchymal strips to CaCl2 (in K+-depolarised tissue), KCl, acetylcholine and histamine. Indomethacin (5 microM) preferentially enhanced the response of tracheal strips from normal animals to histamine and to a lesser extent acetylcholine but not to CaCl2 or KCl. A similar trend was observed in sensitized tissues. Indomethacin pretreatment did not cause changes in responsiveness or sensitivity of lung parenchymal strips from normal or sensitized guinea-pigs to the agonists tested. It is concluded that immunological sensitization produced a non-specific hyperresponsiveness in trachea and lung parenchymal strips. Conversely, cyclooxygenase inhibition by indomethacin elicited a selective increase in the responsiveness to certain agonists in central but not in the peripheral airways.
Cortijo
J
J
Departamento de Farmacologia y Farmacotecnia, Universitat de València, Madrid, Spain.
Ortiz
J L
JL
Sanz
C
C
Sarría
B
B
Pascual
R
R
Perpiñá
M
M
Esplugues
J
J
Morcillo
E J
EJ
D000818:Animals; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D010208:Papaverine / Q000494:pharmacology; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014132:Trachea / Q000187:drug effects
D012710:Serum Albumin, Bovine; D010208:Papaverine; D007213:Indomethacin