Uptake of colchicine, a microtubular system disrupting agent, by isolated rat hepatocytes.
The possible mechanism of hepatic uptake of colchicine (CLC), a microtubule system disrupting agent, was examined using isolated rat hepatocytes. The existence of a carrier-mediated active transport system for CLC was demonstrated. This transport system is saturable, is affected by metabolic inhibitors (dinitrophenol, KCN) and a SH-group blocker (p-hydroxymercuribenzoic acid but not N-ethylmaleimide), and is sensitive to temperature. Ouabain, an inhibitor of Na+, K+-ATPase, does not affect the transport system of CLC.
Wierzba
K
K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Sugiyama
Y
Y
Okudaira
K
K
Iga
T
T
Hanano
M
M
D000818:Animals; D002478:Cells, Cultured; D003078:Colchicine / Q000493:pharmacokinetics* / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000494:pharmacology
D013438:Sulfhydryl Compounds; D003078:Colchicine