An efficient laboratory made apparatus for DNA amplification.
DNA amplification by the polymerase chain reaction (PCR) is a method capable of producing a selective and very high enrichment of a specific DNA sequence. Hence it seems to be useful in various fields from basic research to clinical applications. In order to automatize PCR we assembled for a very low cost a mechanical system designed to carry a test tube holder successively in three thermal baths set at the required temperatures for the reaction. Two examples of the use of this machine are given: (i) amplification of DNA of a particular subtype of acute intermittent porphyria; (ii) the detection of the chimeric c-abl/bcr message found in chronic myelogenous leukemia cells.
Bertrand
O
O
INSERM U.160, Hôpital Beaujon, Clichy, France.
Delfau
M H
MH
Garbarz
M
M
Picat
C
C
Devaux
I
I
Dhermy
D
D
Boivin
P
P
Grandchamp
B
B
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D011164:Porphyrias / Q000235:genetics; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D004247:DNA; D004259:DNA-Directed DNA Polymerase