Concentrations of apolipoproteins B, C-I, C-II, C-III, E and lipids in serum and serum lipoproteins of normal subjects during alimentary lipaemia.
The effect of alimentary lipaemia, induced by ingestion of 100 g of fat (cream), on lipids and apolipoprotein B, C-I, C-II, C-III and E levels was evaluated in 16 normotriglyceridaemic subjects. Apolipoprotein concentrations were determined by enzyme immunoassay in whole serum and, in a subsample of seven subjects, in serum lipoprotein fractions of d less than 1.006 kg/l (supernatant) containing very low density lipoproteins (VLDL) and chylomicrons and of d greater than 1.006 kg/l (infranatant) containing low density lipoproteins (LDL) and high density lipoproteins (HDL). Triglyceride concentrations in the supernatant were increased by 164% 3 h after the fat ingestion. The level of alimentary lipaemia was positively related to the fasting serum concentrations of triglycerides and apolipoproteins C-II and C-III and inversely related to the fasting concentration of apolipoprotein E in the infranatant lipoprotein fraction with d greater than 1.006 kg/l. No significant changes in the serum levels of apolipoproteins B, C-I and E were found during the postprandial lipaemia. By contrast, serum concentrations of apolipoproteins C-II and C-III were decreased (compared to the fasting levels) 6 h after the fatty meal. In the 3-h samples all apolipoproteins were increased in the supernatant (VLDL + chylomicrons) and a corresponding decrease was observed in the infranatant (LDL + HDL). In conclusion, this study shows that in normotriglyceridaemic subjects a high serum level of apo C-II is not associated with a more rapid clearance of alimentary lipaemia than a low level.(ABSTRACT TRUNCATED AT 250 WORDS)
Annuzzi
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G
King Gustaf V Research Institute, Karolinska Institute, Stockholm, Sweden.
Holmquist
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Carlson
L A
LA
D000328:Adult; D053302:Apolipoprotein C-I; D053304:Apolipoprotein C-II; D053305:Apolipoprotein C-III; D001053:Apolipoproteins / Q000097:blood*; D001055:Apolipoproteins B / Q000097:blood; D001056:Apolipoproteins C / Q000097:blood; D001057:Apolipoproteins E / Q000097:blood; D002784:Cholesterol / Q000097:blood*; D003611:Dairy Products; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D014280:Triglycerides / Q000097:blood*
D053302:Apolipoprotein C-I; D053304:Apolipoprotein C-II; D053305:Apolipoprotein C-III; D001053:Apolipoproteins; D001055:Apolipoproteins B; D001056:Apolipoproteins C; D001057:Apolipoproteins E; D004041:Dietary Fats; D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol