Steady-state electrophoresis: a technique for measuring physical properties of macro-ions.
The basic theory of a newly discovered physical technique, steady-state electrophoresis, is described followed by analyses of several representative macro-ion systems with the technique. This method measures apparent moments of molar diminished charge (defined below) of solvated macro-ions from an analysis of a concentration gradient of the macro-ions stabilized against a semipermeable membrane by the opposing forces of diffusion and an external electric field. The experimental results confirm that the theory adequately describes the behavior of the macro-ion systems at electrophoretic steady state and suggest applications for the technique in addition to measuring macro-ion-small ion interactions.
Godfrey
J E
JE
Biology Department, Johns Hopkins University, Baltimore, MD 21218.
D004563:Electrochemistry; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D005969:Glutamate Dehydrogenase; D007477:Ions; D008433:Mathematics; D008962:Models, Theoretical; D012710:Serum Albumin, Bovine
D007477:Ions; D012710:Serum Albumin, Bovine; D005969:Glutamate Dehydrogenase