title: Quantitative determination of 4-hydroxy-4-androstene-3,17-dione (4-OHA), a potent aromatase inhibitor, in human plasma, using isotope dilution mass spectrometry.
abstract: An original method is described for the determination in human plasma of 4-hydroxy-4-androstene-3,17-dione (4-OHA), a potent aromatase inhibitor, by isotope dilution mass-spectrometry using 7,7-[2H2]-4-OHA as internal standard. This compound was synthesized starting from 7,7-[2H2]-4-androstene-3,17-dione. The procedure includes an extraction step using an Extrelut 1 column and a derivatization with N,o-bis(trimethylsilyl)trifluoroacetamide (BSTFA). The minimum detection level of the method is 0.650 pg and the coefficients of variation for the 0.5 ng/ml (plasma) and 5 ng/ml (plasma) concentrations are 3.2% (within assay) and 6.7% (between assay) and 1.86% (within assay) and 2.3% (between assay) respectively.
authors.affiliation: Department of Organic Chemistry, University of Florence, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Guarna
authors.forename: G
authors.initials: G
authors.lastname: Moneti
authors.forename: D
authors.initials: D
authors.lastname: Prucher
authors.forename: R
authors.initials: R
authors.lastname: Salerno
authors.forename: M
authors.initials: M
authors.lastname: Serio
mesh_terms: D000735:Androstenedione / Q000031:analogs & derivatives* / Q000097:blood; D047072:Aromatase Inhibitors; D002849:Chromatography, Gas; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods
chemical_list: D047072:Aromatase Inhibitors; D000735:Androstenedione; C014594:formestane