Cytochemical profile of lymphocytes in haemophilia patients at risk of AIDS.
We report the cytochemical and immunologic features of lymphocytes of 31 patients (26 with haemophilia A and 5 with haemophilia B) chronically treated with commercial clotting factor concentrates. 6 patients, asymptomatic for AIDS were anti-HIV positive. In comparison to aged cross-matched male controls, significant increases of absolute number of lymphocytes and of T and B cell subpopulations with significant reduction of the percentages of CD3, CD4, CD2, CD5, sIg, and CD16 cells was found in haemophiliacs. PAS reaction showed a lower grading score in haemophiliacs and the staining for NBE, AP, NABG revealed a significant reduction of the percentage of the lymphocytes with dot-like positivity in the same patients. The percentage number of AP reactive lymphocytes with dot-like positivity was directly related to the percentage of CD3, and CD4 cells. The anti-HIV positive patients presented lower percentages of CD4 and of dot-like reactive lymphocytes.
Gamba
G
G
Department of Internal Medicine, University of Pavia, Italy.
Ippoliti
G
G
Invernizzi
R
R
Gatti
V
V
Zocchi
M T
MT
Perugini
O
O
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000635:transmission; D001779:Blood Coagulation Factors / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D015483:HIV Antibodies / Q000032:analysis; D006467:Hemophilia A / Q000097:blood* / Q000628:therapy; D002836:Hemophilia B / Q000097:blood* / Q000628:therapy; D006651:Histocytochemistry; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000276:immunology; D008297:Male; D012307:Risk Factors
D001779:Blood Coagulation Factors; D015483:HIV Antibodies