Evidence for proton motive force dependent transport of selenite by Clostridium pasteurianum.
The proton motive force mediated the transport of selenite (SeO3(2-)) in Clostridium pasteurianum cells by proton symport. The proton conductor, carbonyl cyanide m-chlorophenylhydrazone, inhibited SeO3(2-) uptake while N,N'-dicyclohexylcarbodiimide prevented SeO3(2-) uptake by presumably inhibiting the unidirectional ATPase. Acid pulse studies and antibiotic experiments with valinomycin suggest that the transmembrane delta pH component of the proton motive force mediated the transport of SeO3(2-) into the cells. The SeO3(2-) porter system in C. pasteurianum was found to be dependent upon energy source, temperature, and medium pH.
Bryant
R D
RD
Department of Biological Sciences, University of Calgary, Alta., Canada.
Laishley
E J
EJ
D000085:Acetates / Q000494:pharmacology; D001693:Biological Transport, Active; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D003013:Clostridium / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D006851:Hydrochloric Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D020887:Selenious Acid; D012643:Selenium / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D013696:Temperature; D014634:Valinomycin / Q000494:pharmacology
D000085:Acetates; D003470:Culture Media; D014634:Valinomycin; D004024:Dicyclohexylcarbodiimide; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D013395:Sucrose; D020887:Selenious Acid; D012643:Selenium; D006851:Hydrochloric Acid