title: Effect of chronic food restriction in aging rats. II. Liver cytosolic antioxidants and related enzymes.
abstract: The cytosolic status during aging of several antioxidants and enzymatic activities which protect the cell from oxidative damage was explored in the liver of ad libitum-fed and food restricted rats. Restricting calories effectively prevented the age-related decrease in cellular glutathione that occurs in ad libitum-fed rats. Although glutathione reductase exhibited little change with age in ad libitum-fed rats, dietary restriction resulted in greater activity of this enzyme than that of ad libitum-fed animals. Glutathione S-transferase activity of ad libitum-fed rats decreased significantly with age in ad libitum-fed rats but not in food restricted rats. The glutathione peroxidase activity which increased until 12 months in the ad libitum-fed rats declined by 24 months; there was little change with adult age in this enzymatic activity in food restricted rats. Catalase activity declined steadily from 3-24 months in the ad libitum-fed rats, and food restriction prevented this age-related decline. The significance of antioxidants and the related protective enzymes is discussed relative to membrane alterations and the anti-oxidative action of food restriction in relation to age-related degenerative damages.
authors.affiliation: Department of Physiology, University of Texas, San Antonio 78284-7756.
authors.forename: S
authors.initials: S
authors.lastname: Laganiere
authors.forename: B P
authors.initials: BP
authors.lastname: Yu
mesh_terms: D000375:Aging / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism*; D004032:Diet; D005508:Food Deprivation / Q000502:physiology*; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D000975:Antioxidants; D002374:Catalase; D005982:Glutathione Transferase