Simple, rapid, and portable chromatographic tetrazolium reduction method for detection of potassium cyanide in medicinal drugs and confectionery.
A simple, rapid, and portable paper chromatographic method for detection of potassium cyanide in medicinal drugs and a few confectionery samples is described. Potassium cyanide is extracted in methanol and concentrated. Acetone-water-1.5% EDTA (4 + 5.5 + 0.5) mixture is used as the solvent system for paper chromatography. The KCN chromatograms appear as pink spots on paper due to reduction of the chromogenic salt 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride; phenazonium methosulfate is a catalyst. Microgram amounts of KCN can be separated and detected in the laboratory or the marketplace because of the simplicity of the method.
Bhagyalakshmi
K
K
Sri Venkateswara University, Department of Zoology, Tirupati, India.
Kumar
N V
NV
D002854:Chromatography, Paper / Q000379:methods; D003486:Cyanides / Q000032:analysis*; D005504:Food Analysis; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013778:Tetrazolium Salts
D003486:Cyanides; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D013778:Tetrazolium Salts