Catalase activity measurement with the disk flotation method.
Activity measurements of catalase are usually performed with a spectrophotometric method by monitoring the decrease of H2O2 at 240 nm. A different method presented here uses an instrument named Catalase-meter and yields flotation time data which are expressed in tenth of seconds. For the first time, solutions of catalase of different concentrations were tested simultaneously with the two methods, and the Catalase-meter's flotation data were submitted to correlation with international units calculated from spectrophotometric data. The corresponding calibration curve correlates flotation time data to international units. The r2 values thus obtained for the two calibration curves were 0.960 and 0.929, for a range of activity varying from 9.3 to 144.5 international units/ml.
Boismenu
D
D
Institut Armand-Frappier, CRESALA, Laval, Québec, Canada.
Lépine
F
F
Gagnon
M
M
Dugas
H
H
D000818:Animals; D002374:Catalase / Q000032:analysis*; D002417:Cattle; D008099:Liver / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet
D002374:Catalase