Concept of response factor in capillary isotachophoresis. Determination of drugs in solution for intravenous injection.
Twenty-six drugs in solutions for intravenous injection were determined by capillary isotachophoresis with only one calibration point for each component. The maximum deviation of the labelled concentration was 2%. A new calibration constant is introduced, viz., the response factor (RF, dimensionless), which is independent of the diameter of the capillary, the construction of the universal detector and the driving current used during detection. It is shown that the RF can be used on different equipment, using different currents during detection. It appears that the RF is usable for routine analysis when a deviation of 5% is acceptable. Daily one-point recalibration, however, improves this value to 1%.
Gladdines
M M
MM
Laboratory of Instrumental Analysis, Eindhoven University of Technology, The Netherlands.
Reijenga
J C
JC
Trieling
R G
RG
van Thiel
M J
MJ
Everaerts
F M
FM
D004586:Electrophoresis / Q000379:methods*; D007262:Infusions, Intravenous; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000032:analysis*; D012996:Solutions
D004364:Pharmaceutical Preparations; D012996:Solutions