title: Secretion of 35SO4-labeled proteins from isolated rat hepatocytes.
abstract: Sulfation is a Golgi-specific modification of secretory proteins. We have characterized the proteins that are labeled with 35SO4 in cultures of rat hepatocytes and studied their transport to the medium. Analysis by polyacrylamide gel electrophoresis showed that of the five most heavily labeled proteins, four had well-defined mobilities--apparent molecular masses of 188, 142, 125, and 82 kDa--whereas one was electrophoretically heterogeneous--apparent molecular mass of 35-45 kDa. Judging by their relatively high resistance to acid treatment, the sulfate residues in the 125- and 35-45-kDa proteins were linked to carbohydrate. Some of the secreted proteins were sialylated. In samples of pulse-labeled cells, there appeared to be no unsialylated forms, indicating that sulfation occurred after sialylation, presumably in the trans Golgi. Kinetic experiments showed that the cellular half-life was the same for all the sulfated proteins--about 8 min--consistent with the idea that transport from the Golgi complex to the cell surface occurs by liquid bulk flow.
authors.affiliation: Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
authors.forename: M M
authors.initials: MM
authors.lastname: von Würtemberg
authors.forename: E
authors.initials: E
authors.lastname: Fries
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000378:metabolism*; D013462:Sulfur Radioisotopes
chemical_list: D011506:Proteins; D013431:Sulfates; D013462:Sulfur Radioisotopes