Mannostatins A and B: new inhibitors of alpha-D-mannosidase, produced by Streptoverticillium verticillus var. quintum ME3-AG3: taxonomy, production, isolation, physico-chemical properties and biological activities.
Mannostatins have been isolated as part of a program designed to find microorganism-produced inhibitors of alpha-D-mannosidase from Streptoverticillium verticillus var. quintum. They were purified by sequential use of active carbon and Dowex resins and then isolated as colorless powders. Mannostatins A and B have the molecular formula, C6H13NO3S and C6H13NO4S, respectively. They were competitive with the substrate, and the inhibition constants (Ki) of mannostatins A and B were 4.8 x 10(-8) M.
Aoyagi
T
T
Institute of Microbial Chemistry, Tokyo, Japan.
Yamamoto
T
T
Kojiri
K
K
Morishima
H
H
Nagai
M
M
Hamada
M
M
Takeuchi
T
T
Umezawa
H
H
D003517:Cyclopentanes / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D008361:Mannosidases / Q000037:antagonists & inhibitors*; D015394:Molecular Structure; D013171:Spores, Bacterial; D013306:Streptomycetaceae / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D043323:alpha-Mannosidase
D003517:Cyclopentanes; C060107:mannostatin A; C060108:mannostatin B; D008361:Mannosidases; D043323:alpha-Mannosidase