One-step purification of rat plasma alpha 1-acid glycoprotein by antibody affinity chromatography: application to normal and inflamed rat sera.
Most purification procedures used previously to isolate alpha 1-acid glycoprotein (AGP) from plasma can lead to some alterations in its carbohydrate moiety. An immunoaffinity chromatographic method is proposed for purifying in one step rat plasma AGP without any detectable modification of its glycan moiety. Crossed immunoaffinoelectrophoresis with concanavalin A before and after purification showed identical patterns, suggesting no glycan selection during the purification. In the same way no desialylation occurred during the purification step. This immunoaffinity chromatographic procedure provided evidence of a decreased level of fucosyl residues in turpentine oil rat plasma AGP compared with normal rat plasma AGP.
Drechou
A
A
URA CNRS 622, UER des Sciences Pharmaceutiques et Biologiques, Chatenay-Malabry, France.
Perez-Gonzalez
N
N
Biou
D
D
Rouzeau
J D
JD
Feger
J
J
Durand
G
G
D000818:Animals; D002846:Chromatography, Affinity; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007202:Indicators and Reagents; D007249:Inflammation / Q000097:blood*; D008297:Male; D009961:Orosomucoid / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D007202:Indicators and Reagents; D009961:Orosomucoid; D003208:Concanavalin A