Absorption and ester cleavage of methyl salicylate by skin of single-pass perfused rabbit ears.
1. Rabbit ears were single-pass perfused with a cell-free medium. Arterial pressure, oxygen consumption, and lactate production increased with flow rate. 2. Methyl salicylate was hydrolysed with an apparent Vmax of 1.5 nmol/min per cm2 surface area, a rate about 25 times greater than after arterial administration. 3. Estimation of the Km was not possible, due to oedema developing at arterial concentrations greater than 100 microM methyl salicylate. 4. The model appears suitable to compare absorption, as well as metabolic rates, of xenobiotics in rabbit skin.
Behrendt
H
H
Walther-Straub-Institut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität, München, FR Germany.
Kampffmeyer
H G
HG
D000818:Animals; D004423:Ear; D006868:Hydrolysis; D007700:Kinetics; D010477:Perfusion / Q000379:methods; D011817:Rabbits; D012459:Salicylates / Q000378:metabolism / Q000493:pharmacokinetics*; D012869:Skin Absorption / Q000187:drug effects*
D012459:Salicylates; C033069:methyl salicylate