[Intraspecific polymorphism of glucose-6-phosphate dehydrogenase in Amoeba proteus].
Seven Amoeba proteus strains of different origin were studied using a polyacrylamide gel electrophoresis micromethod in the presence of NADP+ in cathode buffer. The strains examined differ in glucose-6-phosphate dehydrogenase (G6PD) zymograms: the majority of them (A, L, F, Da and Bk) display 3 bands, whereas strains B and C show 4 and 2 bands, respectively. All but strain C have a common band of high relative mobility. Strain B shows two fast migrating bands instead of a single band typical of strains A, L, F, Da and Bk. The results obtained testify to a certain intraspecific polymorphism of electrophoretic G6PD patterns in A. proteus. These difference between strains may be used as genetic markers in the nuclear transfer experiments.
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D000656:Amoeba / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D011110:Polymorphism, Genetic; D013045:Species Specificity
D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase
Vnutrividovoĭ polimorfizm gliukozo-6-fosfatdegidrogenazy u ameb Amoeba proteus.