Reaction of Se with SH groups in spectrin is involved in the stabilization of erythrocyte membrane skeleton.
Na2SeO3 supplementation in the dialysis medium could obviously prevent the dissociation of spectrin from the erythrocyte membranes. Such Se effect could be eliminated by pretreatment of erythrocyte membranes with a SH-blocking reagent, iodoacetamide(IAA) or addition of a SH-compound, dithio-threitol. The fluorescence intensity of erythrocyte membranes labelled with the fluorescent probe N-(3-pyrenyl)-maleimide decreased with increasing Na2SeO3 concentration used for pretreatment of ghosts. 31P-NMR spectra of erythrocyte membrane dialyzed in the presence or absence of Na2SeO3 concentration showed a difference in chemical shift anisotropy (delta sigma) between these two samples. These data suggest that the stabilization effect is based on changes in lipid-protein interaction and conformation of membrane skeletal components induced by reaction of their SH groups with Na2SeO3.
Yang
F Y
FY
Institute of Biophysics, Academia Sinica, Beijing, China.
Yang
M Z
MZ
D055598:Chemical Phenomena; D002621:Chemistry; D015314:Dialysis Solutions / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006801:Humans; D007460:Iodoacetamide / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D012643:Selenium / Q000378:metabolism*; D013049:Spectrin / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D015314:Dialysis Solutions; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D013049:Spectrin; D012643:Selenium; D007460:Iodoacetamide