Measurement of cell swelling in a hypotonic medium as a rapid and sensitive test of cell injury.
A development and application of a novel test of cell damage is described. The test is based on the ability of undamaged cells to swell in the hypotonic medium. The test is much more rapid and sensitive than the tests based on staining with trypan blue or fluorescein diacetate.
Kajstura
J
J
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
Reiss
K
K
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis / Q000473:pathology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D007038:Hypotonic Solutions; D008564:Membrane Potentials; D011188:Potassium / Q000032:analysis / Q000493:pharmacokinetics; D012964:Sodium / Q000032:analysis / Q000493:pharmacokinetics; D014882:Water-Electrolyte Balance
D007038:Hypotonic Solutions; D012964:Sodium; D011188:Potassium