title: Binding of prazosin to alpha 1-acid glycoprotein and albumin: effect of protein purity and concentrations.
abstract: Prazosin is extensively bound in human serum/plasma. In the present study a bound fraction of 93-95% was observed at 37 degrees for therapeutic drug concentrations. Both alpha 1-acid glycoprotein (AAG) and albumin (HSA) are established as transport proteins for prazosin, but their individual contribution to the extent and variability of protein binding in serum/plasma is unclear. The present study showed that AAG possesses one binding site per molecule with high affinity (Kd approximately 0.8 microM) for prazosin. HSA, essentially globulin-free, bound prazosin with lower affinity (Kd approximately 30 microM) with an average of 0.3 binding sites per molecule. However, less purified HSA, containing globulins, exhibited apparently higher affinity (Kd approximately 8 microM), but lower binding capacity (0.07 sites per molecule) for prazosin. In mixtures of highly purified proteins, the concentrations of AAG, and not HSA, determined the extent and variability of prazosin binding.
authors.affiliation: Department of Pharmacology, University of Tromsø, Norway.
authors.forename: G
authors.initials: G
authors.lastname: Sager
authors.forename: R
authors.initials: R
authors.lastname: Jaeger
authors.forename: C
authors.initials: C
authors.lastname: Little
mesh_terms: D003956:Dialysis; D006801:Humans; D009961:Orosomucoid / Q000302:isolation & purification / Q000378:metabolism*; D011224:Prazosin / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D009961:Orosomucoid; D012709:Serum Albumin; D011224:Prazosin