A continuous-flow automated assay for iodometric estimation of hydroperoxides.
An iodometric method for the analysis of hydroperoxides has been automated to allow analysis of aqueous biological samples (containing less than 20 mg/ml protein) and lipid hydroperoxide extracts. The evolution of triiodide ions is measured spectrophotometrically at 360 nm. Dependent on the type of sample, 30-60 samples can be analyzed per hour and the system allows detection of less than 100 pmol of peroxide. The assay is linear over a range of 100 pmol to 25 nmol. The sample volume used routinely was 80 microliters.
Thomas
S M
SM
Department of Biology/Biochemistry, Brunel University, Uxbridge, Middlesex, England.
Jessup
W
W
Gebicki
J M
JM
Dean
R T
RT
D000818:Animals; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D007455:Iodine / Q000032:analysis*; D007700:Kinetics; D008054:Lipid Peroxides / Q000032:analysis; D008077:Lipoproteins, LDL / Q000302:isolation & purification; D008081:Liposomes / Q000302:isolation & purification; D008928:Mitochondria / Q000032:analysis; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions
D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D008081:Liposomes; D010713:Phosphatidylcholines; D012996:Solutions; D007455:Iodine; D006861:Hydrogen Peroxide