Differential alterations in dopamine turnover rates in the stalk-median eminence and posterior pituitary during the preovulatory prolactin surge.
The relative contributions of dopamine (DA) and prolactin-releasing factor (PRF) in generating the preovulatory prolactin (PRL) surge were investigated. Immature female rats were injected with pregnant mare's serum gonadotropin (PMSG) on day 28. Jugular blood was collected hourly on days 30 and 31. PRL levels were low in the morning of day 30, rose 10-12 times to peak levels from 14.00 to 16.00 h, reached a prolonged plateau from 18.00 to 24.00 h, and reduced to basal levels in the morning of day 31. All PMSG-treated rats ovulated an average of 13-14 ova. PRL levels in age-matched control rats were low throughout this time, and no oviductal ova were present. DA turnover rates in the stalk-median eminence (SME) and posterior pituitary (PP) were determined from the decline in tissue DA after injecting alpha-methyl-p-tyrosine (alpha-MPT), a competitive inhibitor of tyrosine hydroxylase. DA turnover rates increased or were unaltered in the SME and PP, respectively, during the peak PRL phase as compared to presurge rates. In contrast, DA turnover rates were significantly reduced in both tissues during the plateau phase. The turnover rate in the SME, but not the PP, was increased in the morning of day 31. DA turnover rates in control rats never changed. Injection of alpha-MPT to PMSG-treated rats increased PRL levels at all times examined except during the plateau phase. Blood PRL levels were also determined in PMSG-treated rats following posterior pituitary lobectomy or sham lobectomy. The PRL surge was similar in both groups and all rats ovulated.(ABSTRACT TRUNCATED AT 250 WORDS)
Arbogast
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LA
Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis.
Murai
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Ben-Jonathan
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D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D006736:Horses; D008473:Median Eminence / Q000378:metabolism*; D008781:Methyltyrosines / Q000494:pharmacology; D010060:Ovulation; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000097:blood / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D019805:alpha-Methyltyrosine
D008781:Methyltyrosines; D019805:alpha-Methyltyrosine; D011388:Prolactin; D004298:Dopamine