[Effect of poly(dA).poly(dT) sequence inserted into EcoR1 site of pBr322 plasmid on the expression of tet and bla genes].
The 99 base pair poly(dA).poly(dT) stretch was inserted into EcoRI site of pBR plasmid. The effect of this insertion on tet-gene expression from P2 promoter, and bla-gene expression from P1 and P3 promoters was studied. The insertion results in a slight enhancement of the resistance of the cells to tetracycline and a decreased sensitivity to ampicillin. It is suggested that these findings reflect the effect of insertion on the transcription of the corresponding genes.
Andreeva
I S
IS
Denisova
L Ia
LIa
Zagrebel'nyĭ
S N
SN
Kileva
E V
EV
Pustoshilova
N M
NM
D000668:Ampicillin Resistance / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D005786:Gene Expression Regulation; D010957:Plasmids; D011067:Poly dA-dT; D011089:Polydeoxyribonucleotides; D011401:Promoter Regions, Genetic; D013753:Tetracycline Resistance / Q000235:genetics; D014170:Transformation, Genetic
D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; D004247:DNA; D015246:Deoxyribonuclease EcoRI
Vliianie posledovatel'nosti poli(dA).poli(dT), vstroennoĭ v EcoR1-uchastok plazmidy pBR322, na ékspressiiu genov tet i bla.