A major antigen on the outer envelope of a human oral spirochete, Treponema denticola.
Human oral spirochetes are prominent inhabitants of subgingival plaque in patients with periodontal disease. By immunoelectron microscopy using protein A-gold complexes and either polyclonal mouse antiserum against the 53-kDa antigen or 53-kDa-antigen-specific monoclonal antibody, a major polypeptide antigen, with a molecular weight of 53,000 (molecular size, 53 kilodaltons [kDa]), of a human oral spirochete, Treponema denticola ATCC 33520, was found to localize on the surface of the outer envelope.
Umemoto
T
T
Department of Oral Microbiology, School of Dentistry, Asahi University, Gifu, Japan.
Namikawa
I
I
Suido
H
H
Asai
S
S
D000372:Agglutination Tests; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010510:Periodontal Diseases / Q000382:microbiology / Q000473:pathology; D014208:Treponema / Q000276:immunology* / Q000648:ultrastructure
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface