Monoclonal antibody based immunoassay for human aminoacylase-1.
Aminoacylase-1 is expressed in a wide variety of cell types. Although the exact role of this enzyme in cellular physiology remains unclear, it has been postulated to function in the salvage of acylated aminoacids. The enzyme is also of interest due to its gene map location on chromosome 3p21, a region deleted in several neoplasms. We have produced mouse monoclonal antibodies and rabbit antisera which recognize human aminoacylase-1. A sandwich ELISA has been developed which allows the measurement of human aminoacylase-1.
Miller
Y E
YE
Department of Medicine, Veterans Administration Medical Center, Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado.
Kao
B
B
D000581:Amidohydrolases / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000581:Amidohydrolases; C019672:aminoacylase I