Variation of tandem repeats in the developmentally regulated procyclic acidic repetitive proteins of Trypanosoma brucei.
The procyclic acidic repetitive proteins (PARPs) of Trypanosoma brucei are developmentally regulated surface proteins encoded by a family of polymorphic genes. We have determined the complete nucleotide sequence of a novel member of the PARP gene family and investigated its expression. The amino acid sequence deduced from the parpA alpha gene showed a marked conservation of both the amino- and carboxy-terminal regions compared with other PARPs but revealed the substitution of a pentapeptide for the dipeptide repeating unit that is characteristic of all other PARPs. Northern hybridization analysis indicated that expression of the parpA alpha gene, like that of other members of this gene family, is confined to the procyclic stage of the T. brucei life cycle. This result implies coordinate regulation of the unlinked genetic loci that encode PARPs.
Mowatt
M R
MR
Rockefeller University, New York, New York 10021.
Wisdom
G S
GS
Clayton
C E
CE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D014644:Genetic Variation; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins; D012091:Repetitive Sequences, Nucleic Acid; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development
D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; C053277:procyclic acidic repetitive protein, Trypanosoma; D004247:DNA