Preparation of microvillus membrane vesicles from the intestine of embryonic and young chicks.
1. A procedure is described for the isolation of microvillus membranes from 19-day old embryonic, newly hatched, and 2-day old chicken intestine. 2. The magnesium concentration of epithelial cell homogenates is shown to be a crucial factor in obtaining membranes of equal purity from the three age groups. 3. Microvillus membranes are purified 20-25 fold over the original homogenate and form vesicles which are tightly sealed based on the Na+-dependent accumulation of glucose to levels four to five times equilibrium values. 4. These membrane preparations should prove useful in future studies concerning the embryonic and neonatal development of microvillus enzymes and nutrient transport systems in the chicken.
Black
B L
BL
Department of Zoology, North Carolina State University, Raleigh 27695-7617.
Rogers
J O
JO
Smith
J E
JE
D000818:Animals; D000831:Animals, Newborn; D001693:Biological Transport, Active; D002458:Cell Fractionation / Q000379:methods*; D002642:Chick Embryo; D002645:Chickens; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism / Q000648:ultrastructure*; D008274:Magnesium; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure*; D012964:Sodium / Q000378:metabolism
D012964:Sodium; D008274:Magnesium; D005947:Glucose