Mechanism of staphylococcal serine proteinase inactivation by lymphocytes and granulocytes.
Stricking differences were observed in the mechanism of interaction between staphylococcal serine proteinase and surface of human granulocytes or lymphocytes despite the fact that incubation of this enzyme with both types of cells leads to analogical decrease of proteinase activity. Interaction of proteinase with lymphocytes releases peptides smaller than these released spontaneously by non-treated lymphocytes or lymphocytes treated with DFP-proteinase. However, in supernatants of lymphocytes neither complex of proteinase with cell derived molecules nor changes of electrophoretic mobility of proteinase was found. Products of proteinase-lymphocyte reaction have a proliferative effect on intact lymphocytes, which is greater that the one of active proteinase. On the other hand granulocytes are resistant to proteinase and bind active proteinase as well as the DFP-proteinase in the receptor mediated way, followed by endocytosis with the affinity similar to the one in monocytes.
Ochałek
T
T
Department of Microbiology and Immunology, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Turyna
B
B
Porwit-Bóbr
Z
Z
D004591:Electrophoresis, Polyacrylamide Gel; D004705:Endocytosis; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D007457:Iodine Radioisotopes; D007531:Isoflurophate / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000502:physiology*; D011971:Receptors, Immunologic / Q000187:drug effects / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000276:immunology
D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic; D015842:Serine Proteinase Inhibitors; D007531:Isoflurophate; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase