Interactions of the dimethyldiazaperopyrenium dication with nucleic acids. 2. Binding to double-stranded polynucleotides.
The interactions of dimethyldiazaperopyrenium dication (1) with DNA have been studied by spectroscopic methods: absorption, static and dynamic fluorescence, and linear dichroism. 1 binds strongly to DNA at 250 mM NaCl, with a higher affinity for G-C pairs as compared to A-T pairs. The dye fluorescence is enhanced when it is bound to A-T pairs, whereas the emission is quenched in the vicinity of G-C pairs. Evidence for intercalation has been obtained via energy transfer and linear dichroism measurements.
Slama-Schwok
A
A
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, Paris, France.
Rougée
M
M
Ibanez
V
V
Geacintov
N E
NE
Montenay-Garestier
T
T
Lehn
J M
JM
Hélène
C
C
D001482:Base Composition; D001665:Binding Sites; D001667:Binding, Competitive; D004735:Energy Transfer; D015394:Molecular Structure; D009691:Nucleic Acid Denaturation; D009696:Nucleic Acids; D009994:Osmolar Concentration; D011119:Polynucleotides; D011806:Quinolinium Compounds; D013053:Spectrophotometry
D009696:Nucleic Acids; D011119:Polynucleotides; D011806:Quinolinium Compounds; C060279:dimethyldiazaperopyrenium