Ultrastructure of hepatic and renal lesions in chickens fed aflatoxin.
Male broiler chicks were given feed and water ad libitum from hatching through 3 weeks of age. The feed contained 0, 1.25, 2.5, and 5.0 micrograms of aflatoxin/g of feed. The chicks were killed by cervical dislocation and specimens of liver and kidney were obtained for electron microscopy on days 3, 6, 9, 17, and 21. In chicks fed 5.0 micrograms of aflatoxin, the primary lesions in liver were hepatocellular lipidosis, enlargement of bile canaliculi, reduction in mitochondrial size, mild lymphocytic infiltration, and hepatocellular degeneration and necrosis. Similar lesions were noticed in some chicks fed 2.5 micrograms of aflatoxin, but none was observed in chicks fed at 1.25 micrograms of aflatoxin. At 5 micrograms of aflatoxin, the most consistent lesion in the kidney was thickening of the glomerular basement membrane. Similar glomerular lesions were observed at 2.5 micrograms of aflatoxin, but not at 1.25 micrograms of aflatoxin. Some foot processes of the glomerular epithelial cells were poorly developed. Fusion of foot processes was not observed and fibrous material was not evident in the basement membrane. The pseudopodia of endothelial cells lining the thickened basement membrane were depleted in number or were absent. Degenerative changes also were observed in the cells of the proximal convoluted tubules, but these were less consistent than those of the glomerulus.
Mollenhauer
H H
HH
Veterinary Toxicology and Entomology Research Laboratory, College Station, TX 77841.
Corrier
D E
DE
Huff
W E
WE
Kubena
L F
LF
Harvey
R B
RB
Droleskey
R E
RE
D000818:Animals; D002645:Chickens; D007668:Kidney / Q000648:ultrastructure*; D007678:Kidney Glomerulus / Q000648:ultrastructure; D007684:Kidney Tubules / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D011201:Poultry Diseases / Q000473:pathology*; D000096702:Aflatoxin Poisoning