Automated zone-electrophoretic sample treatment for the analysis of biological samples by high-performance liquid chromatography.
The automation of zone-electrophoretic sample treatment for liquid chromatography is described. The procedure is completely controlled from a liquid chromatograph. The carry-over of proteins from human serum under different experimental conditions was studied. The influence of the presence of proteins in the sample is illustrated with the anionic compound salicylic acid and the increase in selectivity for cationic compounds is demonstrated with the determination of ephedrine, norephedrine and amphetamine in urine.
Debets
A J
AJ
Laboratory for Analytical Chemistry, Free University, Amsterdam, The Netherlands.
Frei
R W
RW
Hupe
K P
KP
Kok
W T
WT
D000661:Amphetamine / Q000652:urine; D001798:Blood Proteins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D004809:Ephedrine / Q000652:urine; D006801:Humans; D010665:Phenylpropanolamine / Q000652:urine; D012459:Salicylates / Q000097:blood
D001798:Blood Proteins; D012459:Salicylates; D010665:Phenylpropanolamine; D000661:Amphetamine; D004809:Ephedrine