[Use of probes containing cloned genes of the Karelian fever virus in detecting viral genetic material in infected cells by a molecular hybridization method].
Two plasmid DNA-probes containing DNA-replicas of KFV genes (clone 1-protein E1 gene, clone 9--proteins E1 and P1 genes of KFV) were used for detection of the genetic material of Karelian fever virus (KFV) in the infected cells and study of the time course of accumulation of virus-specific RNAs in the process of infection. The detection was performed by the method of RNA:DNA dot-hybridization. Both probes were hybridized with KFV and Sindbis virus RNA in equal amounts--5 X 10(2) infected cells at the peak of virus infection (12 hours). None of the probes used could be bound with RNA of Venezuelan equine encephalomyelitis virus. The results obtained by the dot-hybridization method agree with previously published data on the antigenic relationship between Sindbis virus and KFV.
Uryvaev
L V
LV
Rusavskaia
E A
EA
Samokhvalov
E I
EI
Ionova
K S
KS
Parasiuk
N K
NK
D002478:Cells, Cultured; D015342:DNA Probes; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics*; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D014162:Transfection
D015342:DNA Probes; D012367:RNA, Viral
Ispol'zovanie zondov, soderzhashchikh klonirovannyye geny virusa karel'skov likhoradki, dlia vyiavleniia geneticheskogo materiala virusa v zarazhennykh kletkakh metodom molekuliarnoĭ gibridizatsii.