Genetic and biochemical characterization of the thymidine kinase gene from herpesvirus of turkeys.
The thymidine kinase gene encoded by herpesvirus of turkeys has been identified and characterized. A viral mutant (ATR0) resistant to 1-beta-D-arabinofuranosylthymine was isolated. This mutant was also resistant to 1-(2-fluoro-2-deoxy-beta-D-arabinofuronosyl)-5-methyluracil and was unable to incorporate [125I]deoxycytidine into DNA. The mutant phenotype was rescued by a cloned region of the turkey herpesvirus genome whose DNA sequence was found to contain an open reading frame similar to that for known thymidine kinases from other viruses. When expressed in Escherichia coli, this open reading frame complemented a thymidine kinase-deficient strain and resulted in thymidine kinase activity in extracts assayed in vitro.
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Synergen Inc., Boulder, Colorado 80301.
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Bandyopadhyay
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D004279:DNA, Viral / Q000235:genetics*; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006564:Herpesviridae / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D013937:Thymidine Kinase / Q000235:genetics*; D014422:Turkeys
D003470:Culture Media; D004279:DNA, Viral; D013937:Thymidine Kinase