Detection of antigens in urine during acute toxoplasmosis.
Toxoplasma antigens were detected in sera and urine of mice acutely infected with Toxoplasma gondii. The concentrations of antigens in the urine samples measured by enzyme-linked immunosorbent assay were similar to those detected in the sera of the corresponding mice. The major antigens were not dialyzable and were largely destroyed by treatment with trichloroacetic acid and heat (100 degrees C for 1 h). Toxoplasma antigens were demonstrable on Western blots (immunoblots) of the urine samples.
Huskinson
J
J
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.
Stepick-Biek
P
P
Remington
J S
JS
D000208:Acute Disease; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis / Q000652:urine*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D014124:Toxoplasmosis, Animal / Q000175:diagnosis / Q000276:immunology*
D000953:Antigens, Protozoan