title: Establishment of an etoposide (VP-16)-resistant subline of THP-1 human monocytic leukemia cell line.
abstract: A VP-16-resistant subline was established from THP-1 human monocytic leukemia cells by subculturing in stepwise increasing concentrations of the drug. The resistant cells (THP-1/E) were capable of sustaining continuous growth in 1.0 microgram/ml VP-16. The 50% inhibition dose (IC50) was 1.4 micrograms/ml or a 35-fold of the parent cells. A more than 18-fold IC50 was also obtained with teniposide (VM-26). However, no substantial cross-resistance was observed with 5 other antineoplastic drugs, IC50 values being limited below several times of THP-1.
authors.affiliation: Department of Internal Medicine, Tohoku University, Sendai.
authors.forename: Y
authors.initials: Y
authors.lastname: Saijo
authors.forename: N
authors.initials: N
authors.lastname: Kumano
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Koinumaru
authors.forename: S
authors.initials: S
authors.lastname: Sugawara
authors.forename: K
authors.initials: K
authors.lastname: Oizumi
authors.forename: M
authors.initials: M
authors.lastname: Motomiya
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004351:Drug Resistance; D005047:Etoposide / Q000494:pharmacology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
chemical_list: D005047:Etoposide