title: [Substrate specificity of T4 RNA-ligase. The effect of the nucleotide composition of substrates and the size of phosphate donor on the effectiveness of intermolecular ligation].
abstract: Nucleoside 2' (3'),5'-diphosphates, dinucleotides pApA, pApC, pApU, pGpC, pCpC, pUpU (phosphate donors), and trinucleoside diphosphates, such as NpCpC, NpCpU, NpUpC, NpUpU and GpApN (N = U, C, A or G; phosphate acceptors) were used to study the substrate specificity of T4 RNA ligase. Relative efficiency of the mono- and dinucleotide donors depends on the 5'-terminal nucleoside moiety of the dinucleotide: upon ligation with the minimal phosphate acceptor GpUpC, dinucleotides pApA, pApC, and pApU are more effective than nucleotide diphosphate pAp; pGpC is more effective than pGp; efficiencies of pCpC and pCp are almost identical, and efficiency of pUpU is slightly lower than that of pUp. In relative efficiency, dinucleotide donors, varying only in 5'-terminal unit, do not correspond to mononucleotides: pApC greater than pCpC greater than pGpC and pCp greater than pUp approximately pAp much greater than pGp. The effects observed for homooligomeric substrates cannot be extra-polated on heterooligomers.
authors.forename: S M
authors.initials: SM
authors.lastname: Zhenodarova
authors.forename: V P
authors.initials: VP
authors.lastname: Kliagina
authors.forename: F G
authors.initials: FG
authors.lastname: Maistrenko
authors.forename: N M
authors.initials: NM
authors.lastname: Pustoshilova
authors.forename: O A
authors.initials: OA
authors.lastname: Smolianinova
mesh_terms: D002848:Chromatography, DEAE-Cellulose; D008024:Ligands; D009711:Nucleotides; D010710:Phosphates; D011118:Polynucleotide Ligases / Q000378:metabolism*; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D013379:Substrate Specificity; D013604:T-Phages / Q000201:enzymology*
chemical_list: D008024:Ligands; D009711:Nucleotides; D010710:Phosphates; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP)
vernacular_title: Substratnaia spetsifichnost RNK-ligazy T4. Vliianie nukleotidnogo sostava substratov i razmera donora fosfata na éffektivnost' mezhmolekuliarnogo ligirovaniia.