Dynamical structure of glutamate dehydrogenase as monitored by tryptophan phosphorescence. Signal transmission following binding of allosteric effectors.
Changes in conformation of glutamate dehydrogenase from beef liver as a result of interactions with allosteric effectors have been demonstrated from the phosphorescence emission of tryptophan. The triplet state lifetime shows that whereas activators ADP and L-leucine enhance considerably the rigidity of the protein structure surrounding the chromophore, inhibitors GTP, Zn2+ and Ag+ act in an opposite manner increasing the flexibility of this region of the macromolecule. Such changes in dynamical structure of the protein are confirmed independently for the ADP and GTP complexes by oxygen diffusion studies. Phosphorescence lifetime measurements at various protein concentrations and with the enzyme crosslinked by glutaraldehyde demonstrate that ADP and GTP exert the same effect on the structure of the protein regardless of its degree of polymerization. The connection between changes in protein structure and regulatory function is strengthened by the finding that (1) ligands with no regulatory function (Eu3+) do not affect protein structure; (2) pairs of opposite effectors which neutralize each other's influence on catalytic activity do restore an apparent native-like structure in the enzyme. Mutual neutralization and the observation that ADP and GTP display maximum activity at partial saturation of the binding sites has been interpreted in terms of a model which assumes asymmetry in the hexameric enzyme at the trimer level. Evidence for the existence of conformational heterogeneity among the subunits of the enzyme has been provided.
Cioni
P
P
CNR, Istituto di Biofisica, Pisa, Italy.
Strambini
G B
GB
D000244:Adenosine Diphosphate / Q000378:metabolism; D000495:Allosteric Site; D000818:Animals; D002417:Cattle; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D008163:Luminescent Measurements; D046911:Macromolecular Substances; D010100:Oxygen / Q000378:metabolism; D011487:Protein Conformation; D014364:Tryptophan
D046911:Macromolecular Substances; D000244:Adenosine Diphosphate; D006160:Guanosine Triphosphate; D014364:Tryptophan; D005969:Glutamate Dehydrogenase; D010100:Oxygen