Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport.
In reconstitution studies N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits both fusion of endocytic vesicles and vesicular transport in the Golgi apparatus. We show here that the same N-ethylmaleimide-sensitive factor that catalyses the vesicle-mediated transport within Golgi stacks is also required for endocytic vesicle fusion. Thus, it is likely that a common mechanism for vesicle fusion exists for both the secretory and endocytic pathways of eukaryotic cells.
Diaz
R
R
Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110.
Mayorga
L S
LS
Weidman
P J
PJ
Rothman
J E
JE
Stahl
P D
PD
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D004705:Endocytosis; D005033:Ethylmaleimide / Q000494:pharmacology*; D006056:Golgi Apparatus / Q000378:metabolism*; D007106:Immune Sera; D007700:Kinetics; D052066:N-Ethylmaleimide-Sensitive Proteins; D033921:Vesicular Transport Proteins
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins; D007106:Immune Sera; D033921:Vesicular Transport Proteins; D052066:N-Ethylmaleimide-Sensitive Proteins; D005033:Ethylmaleimide