In vitro sensitivity of normal and leukemic myeloid clonogenic cells to hyperthermia: absence of selective effect.
The in vitro heat sensitivity of myeloid clonogenic cells was tested in 22 normal marrows (granulo-monocytic progenitors) and in 40 marrows of patients with acute myelogenous leukemia (leukemic progenitors). Cells were treated for 1 h at 42 degrees, 43 degrees, 44 degrees, or 45 degrees C prior to plating. A temperature-dependent inhibition of growth was seen without a selective effect on the two kinds of progenitors. Because these two kinds of progenitors have the same heat sensitivity, hyperthermia should not be used alone as a technique for in vitro depletion of residual myeloid leukemic cells.
Marie
J P
JP
Service d'Hématologie de l'Hôtel-Dieu, Paris, France.
Thevenin
D
D
Zittoun
R
R
D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D006979:Hyperthermia, Induced; D066298:In Vitro Techniques; D015470:Leukemia, Myeloid, Acute / Q000628:therapy*; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay