The 9-fluorenylmethyloxycarbonyl group as a 5'-OH protection in oligonucleotide synthesis.
Oligo-DNAs are synthesized on a solid support using the 9-fluorenylmethyloxycarbonyl group as a 5'-OH base labile protection. The synthesis of the pure protected nucleotides, a relevant phosphoramidite-type strategy of coupling, and the optimization of the deprotection steps are described. This new synthetic method is an alternative to the standard protocol that avoids acidic conditions.
Ma
Y
Y
Sonveaux
E
E
D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes; D006878:Hydroxides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*
D005449:Fluorenes; D006878:Hydroxides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; C060253:(9-fluorenylmethyl)ethylcarbonate