title: Neurogenesis of basal forebrain cholinergic neurons in rat.
abstract: The basal forebrain cholinergic system embodies a heterogeneous group of neurons distributed in the basal telencephalon that project topographically to the cortical mantle. We sought to examine the generation of these neurons to determine whether basal forebrain neurons have unique patterns of neurogenesis or, if, in contrast, they are born along general neurogenic gradients. The techniques of tritiated thymidine autoradiography and choline acetyltransferase (ChAT) immunocytochemistry were combined to determine the birthdays and neurogenic gradients of cholinergic cells in this region of rat brain. Cholinergic neurogenesis throughout the basal forebrain ranged from embryonic days 12 to 17 (E12-17). Neurogenesis in the nucleus basalis magnocellularis occurred over E12-16, with a peak day of generation on E13. The horizontal limb nucleus of the diagonal band which is located rostral to the nucleus basalis was generated over E12-17, with the majority of cells arising on E14-15. The rostral-most nuclei of the basal forebrain cholinergic system, the vertical limb of the diagonal band and the medial septum, were generated between E13-17, with peak days of neurogenesis on E15 and E15-16, respectively. These results were evaluated quantitatively and demonstrated that the basal forebrain cholinergic neurons were generated along the general caudal-to-rostral gradient previously described for all neurons in this brain region. The results of this study, in combination with those of similar investigations, emphasize that position-dependent epigenetic factors appear to be more potent determinants of the time of neuronal origin than factors which influence a cell's transmitter phenotype.
authors.affiliation: Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010.
authors.forename: D R
authors.initials: DR
authors.lastname: Brady
authors.forename: P E
authors.initials: PE
authors.lastname: Phelps
authors.forename: J E
authors.initials: JE
authors.lastname: Vaughn
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000166:cytology / Q000196:embryology*; D002454:Cell Differentiation; D002799:Cholinergic Fibers / Q000166:cytology / Q000502:physiology*; D005314:Embryonic and Fetal Development; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine
chemical_list: D013936:Thymidine