Ultrafiltration of human serum to determine the size of species that poison voltammetric electrodes.
Solutions containing the electroactive species tris(2,2'-bipyridine)-ruthenium dication and ferrocene carboxylate as well as bovine serum albumin (BSA), gamma globulin (IgG) or human serum were ultrafiltered. Cyclic voltammetry at a glassy carbon electrode showed that the deleterious effects of BSA and IgG were eliminated by passing the solution through a 30 kdalton filter. This was insufficient filtration of serum, however. Ultrafiltration with 3 or 5 kdalton filters was required for good voltammetry. This indicates that the removal of the preponderant proteins is necessary, but not sufficient, to protect electrochemical surfaces.
Elbicki
J M
JM
Department of Chemistry, University of Pittsburgh, PA 15260.
Weber
S G
SG
D015374:Biosensing Techniques; D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000302:isolation & purification; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D008970:Molecular Weight; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D012996:Solutions; D014462:Ultrafiltration
D001798:Blood Proteins; D007074:Immunoglobulin G; D012996:Solutions; D012710:Serum Albumin, Bovine