Depressed translational activity in the androgen sensitive levator ani muscle of the rat.
The androgen-dependent levator ani (LA) muscle of the rat provides a suitable model to explore the molecular mechanism of steroid hormone action in target tissues. The objective of the present series of experiments was to study the effect of gonadectomy (GDX) and androgen replacement therapy on the in vitro protein synthetic capacity of the LA muscle. The incorporation of labeled methionine into the contractile protein fraction of the LA muscle maintained in organ culture decreases in a time-dependent manner following GDX. Translation of total polyadenylated mRNA in the rabbit reticulocyte translation system revealed that the decrease in protein synthetic capacity was not associated with differences in the template activity of the mRNA derived from GDX tissue. However, when polyribosomes were used to direct the same in vitro synthesis system, a significant time-dependent loss of translational activity was observed following GDX. The polyribosomes of the LA muscle of control and GDX rats were shown to contain equivalent amount of rRNA and mRNA of comparable translation efficiency. Collectively the results of these experiments indicate that the decrease in protein synthetic capacity of the LA muscle in androgen deficient rats is due, in part, to a repression of the translation process associated to the functional integrity of polyribosomes.
Boissonneault
G
G
Laval University Hospital Center, Laboratory of Hormonal Bioregulation, Sainte-Foy, Quebec, Canada.
Gagnon
J
J
Ho-Kim
M A
MA
Rogers
P A
PA
Tremblay
R R
RR
D000818:Animals; D001284:Atrophy; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009919:Orchiectomy; D014176:Protein Biosynthesis / Q000187:drug effects; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
D011506:Proteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D013739:Testosterone