Localization and characterization of the glycosylation site of human pancreatic elastase 1.
Crystalline elastase 1 from human pancreas was digested with trypsin. Two peptides, containing the potential N-glycosylation sites at Asn-86 and Asn-125, were isolated and analyzed by amino acid analysis, sequencing and carbohydrate component analysis. The results demonstrate that only Asn-86 is glycosylated.
Wendorf
P
P
Institut für Medizinische Mikrobiologie der Universität, Giessen, FRG.
Geyer
R
R
Sziegoleit
A
A
Linder
D
D
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D006020:Glycopeptides / Q000302:isolation & purification; D006031:Glycosylation; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000378:metabolism*
D000596:Amino Acids; D006020:Glycopeptides; D010196:Pancreatic Elastase