title: Detection and quantification of ligand leakage from lectin affinity columns.
abstract: The usefulness of lectin affinity chromatography for the preparation of glycoproteins is impaired by ligand release. Ligand leakage from mistletoe lectin (MLI) Sepharose 4B column was detected by 24 h skin reaction in mice and by immunoblotting. Immunoaffinity chromatography was found to be an efficient method for the separation of lectin traces from the glycoprotein fraction. A sugar concentration dependent increase of lectin release from MLI-Sepharose 4B column was detected by a solid phase enzyme immunoassay.
authors.affiliation: Institute of Biochemistry, Wilhelm-Pieck University, Rostock, GDR.
authors.forename: H
authors.initials: H
authors.lastname: Walzel
authors.forename: J
authors.initials: J
authors.lastname: Brock
authors.forename: H
authors.initials: H
authors.lastname: Franz
authors.forename: P
authors.initials: P
authors.lastname: Ziska
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D005354:Fibrosarcoma / Q000032:analysis; D006023:Glycoproteins / Q000302:isolation & purification*; D037102:Lectins / Q000302:isolation & purification; D008024:Ligands; D051379:Mice; D008808:Mice, Inbred CBA; D014784:Mistletoe; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D010946:Plants, Medicinal; D054790:Ribosome Inactivating Proteins, Type 2; D012513:Sarcoma, Experimental / Q000032:analysis; D012882:Skin Tests; D014118:Toxins, Biological / Q000302:isolation & purification / Q000633:toxicity
chemical_list: D006023:Glycoproteins; D037102:Lectins; D008024:Ligands; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum