Platelet-activating factor and laser trauma of the iris.
Local application of platelet-activating factor (PAF) on the rabbit eye caused a dose-dependent significant increase in intraocular pressure (IOP). After laser irradiation of the iris the IOP showed a hypertensive phase of about 3 hr. Prophylactic treatment with the PAF antagonist BN 52021 but not with indomethacin abolished the hypertensive phase. Elevated levels of protein (10.6 +/- 0.9 g/l) and prostaglandin E2 (PGE2, 1.7 +/- 0.2 ng/ml) were measured in the aqueous humor 2 hr after laser irradiation of the iris. Prophylactic treatment with BN 52021 showed lower levels of protein (6.1 +/- 0.7) and PGE2 (1.1 +/- 0.02); with indomethacin pretreatment the level of protein was 3.4 +/- 0.7 g/l and of PGE2 0.10 +/- 0.02 ng/ml. A role of PAF as a mediator in ocular inflammatory response is suggested.
Verbey
N L
NL
Eye Hospital, Rotterdam, The Netherlands.
Van Delft
J L
JL
Van Haeringen
N J
NJ
Braquet
P
P
D000818:Animals; D004224:Diterpenes; D004305:Dose-Response Relationship, Drug; D046934:Ginkgolides; D007429:Intraocular Pressure / Q000187:drug effects / Q000528:radiation effects; D007498:Iris / Q000293:injuries*; D007783:Lactones / Q000494:pharmacology; D007834:Lasers / Q000009:adverse effects*; D008297:Male; D009798:Ocular Hypertension / Q000209:etiology; D010936:Plant Extracts / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011817:Rabbits; D011833:Radiation Injuries, Experimental / Q000517:prevention & control
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010936:Plant Extracts; D010972:Platelet Activating Factor; C045856:ginkgolide B