31P NMR studies on rejuvenation of outdated red blood cells: complete regeneration of ATP is accompanied by partial Mg-ATP recomplexation.
We used 31P Nuclear Magnetic Resonance (NMR) spectroscopy to investigate whether regeneration of ATP in outdated human blood is accompanied by Mg-ATP recomplexation. We found that the loss of 'total ATP' (i.e. the sum of ATP4- + MG-ATP2-) which occurs during blood storage is completely reversible by treatment of RBC with precursors for purine nucleotide salvage. The magnesium-ATP complex, however, is only partially (70%) recovered due to a continuous decline of cytosolic free Mg2+ with increasing RBC age. Total RBC magnesium, as measured by flame absorption spectroscopy, did not show any significant change under these conditions.
van Waarde
A
A
Department of Animal Physiology, Gorlaeus Laboratories, University of Leiden, The Netherlands.
Lombarts
A
A
van den Thillart
G
G
Erkelens
C
C
Lugtenburg
J
J
D000255:Adenosine Triphosphate / Q000097:blood*; D001793:Blood Preservation; D003080:Cold Temperature; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy
D000255:Adenosine Triphosphate; D008274:Magnesium