Heat shock stress is deleterious to CNS cultured neurons microinjected with anti-HSP70 antibodies.
CNS cultured neurons microinjected with anti-mouse immunoglobulin or anti-hsp70 antibodies and cultured at 37 degrees C remained healthy while those injected with anti-hsp70 antibodies, exposed to 50 degrees C for 15 min and returned to 37 degrees C exhibited deleterious effects i.e. lesser interconnecting neurites and poor neuronal population. This shows that heat shock stress affects the survival of neurons in vitro.
Khan
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NA
Unité Fonctionnelle de Biologie Cellulaire, C.H.U., Rennes, France.
Sotelo
J
J
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature / Q000009:adverse effects*; D007106:Immune Sera / Q000494:pharmacology*; D051379:Mice; D013312:Stress, Physiological / Q000378:metabolism*
D006360:Heat-Shock Proteins; D007106:Immune Sera