Cloning, expression and chromosomal localization of a member of the human cytochrome P450IIC gene sub-family.
We have isolated and sequenced a cDNA clone (pB8) that codes for a novel member of the cytochrome P450IIC sub-family of man. Analysis, by Southern blot hybridization, of DNA isolated from a panel of nine independent human-rodent somatic cell hybrids demonstrated that the corresponding gene (CYP2C) is located on human chromosome 10. Northern blot hybridization of RNA isolated from human livers revealed a 10-fold inter-individual variation in the expression of the gene.
Shephard
E A
EA
Department of Biochemistry, University College London.
Phillips
I R
IR
Santisteban
I
I
Palmer
C N
CN
Povey
S
S
D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000032:analysis; D005796:Genes; D006801:Humans; D008099:Liver / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D004247:DNA; D003577:Cytochrome P-450 Enzyme System