title: [A study of the cloned human erythropoietin gene expression in mammalian cells in vitro].
abstract: The human gene coding for the principal factor of erythroid cells differentiation, erythropoietin, has been isolated from the genomic phage library using an oligonucleotide probe for the gene. The construction of series of plasmids carrying the erythropoietin gene under the control of various regulatory elements is reported. Efficiency of the erythropoietin gene expression was estimated by testing of the biologically active erythropoietin in conditioned media 48 h after transient transfection of COS 1 and CHO cell lines.
authors.forename: I A
authors.initials: IA
authors.lastname: Kramerov
authors.forename: M G
authors.initials: MG
authors.lastname: Zelenin
authors.forename: N S
authors.initials: NS
authors.lastname: Filiakina
authors.forename: S L
authors.initials: SL
authors.lastname: Kolobkov
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D004921:Erythropoietin / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D014162:Transfection
chemical_list: D004921:Erythropoietin; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII
vernacular_title: Izuchenie ékspressii klonirovannogo gena éritropŏétina cheloveka v kletkakh mlekopitaiushchikh in vitro.