title: Effects of sodium removal on calcium mobilization and dense granule secretion induced by thrombin in human platelets.
abstract: Removal of extracellular sodium decreased calcium mobilization from intracellular stores induced by thrombin in aspirin-treated human platelets. ATP and serotonin secretion were also significantly reduced. Secretion was positively correlated with calcium mobilization, but the presence or absence of sodium did not modify the slope of the regression line. Half-maximal secretion was reached when [Ca2+]i was increased by about 0.1 microM. Calcium mobilization induced by the divalent cation ionophore ionomycin was not modified by sodium removal. Secretion induced by ionomycin was much smaller than the thrombin-induced one for the same increases of [Ca2+]i. These results suggest that the presence of external sodium is required for normal thrombin-induced calcium release from the intracellular stores and hence for dense granule secretion. However, secretion cannot be only attributed to the increase of cell [Ca2+]i but also to other process(es) which are not affected by external sodium.
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de Medicina, Universidad de Valladolid, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Alonso
authors.forename: A
authors.initials: A
authors.lastname: Sanchez
authors.forename: J
authors.initials: J
authors.lastname: García-Sancho
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D002118:Calcium / Q000097:blood*; D003593:Cytoplasm / Q000502:physiology; D003594:Cytoplasmic Granules / Q000502:physiology; D004987:Ethers / Q000494:pharmacology; D005089:Exocytosis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015759:Ionomycin; D012701:Serotonin / Q000378:metabolism*; D012964:Sodium / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology
chemical_list: D004987:Ethers; D012701:Serotonin; D015759:Ionomycin; D000255:Adenosine Triphosphate; D012964:Sodium; D013917:Thrombin; D001241:Aspirin; D002118:Calcium