Effect of extracellular Ca2+ and Mg2+ on enzyme release from quiescent fibroblasts during various exposures.
Release of enzymes from human fibroblasts, as a result of either ATP depletion caused by metabolic inhibitors or a direct cell membrane damage, was increased in the absence of extracellular Ca2+. The lack of extracellular Mg2+ resulted only in a small nonsignificant increase of enzyme release during ATP depletion. However, in a Ca2+-free medium the enzyme release was very much enhanced during ATP depletion if Mg2+ was absent concomitantly. The data show that extracellular calcium protects the cells from cell damage, and that the presence of extracellular magnesium in a calcium-free medium protects the cell against energy-dependent cell damage.
Kristensen
S R
SR
Department of Clinical Chemistry, Odense University Hospital, Denmark.
Hørder
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*
D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D008274:Magnesium; D002118:Calcium