title: Axonal degeneration and regeneration in sensory roots in a genital herpes model.
abstract: In a mouse model of genital herpes simplex virus type 2 (HSV-2) infection, roots of the lower spinal cord were examined 5 days to 6 months after inoculation. Using immunoperoxidase methods on paraffin sections, viral antigen was found in sensory ganglia, their proximal roots and distal nerves on days 5 and 6 after infection. In Epon sections, most mice had focal sensory root abnormalities in lower thoracic, lumbar or sacral levels. At days 7 and 10, lesions showed chiefly nerve fiber degeneration, particularly of large myelinated fibers. At 2 weeks, lesions contained relatively large bundles of small unmyelinated fibers with immature axon-Schwann cell relationships. From 3 to 6 weeks, lesions again contained many more small unmyelinated fibers than normal but, in increasing proportions, axons in bundles were isolated from their neighbors by Schwann cell cytoplasm, and Schwann cells having 1:1 relationships with axons showed mesaxon or thin myelin sheath formation. At later times, the proportion of small unmyelinated axons decreased in parallel with increased numbers of small myelinated axons. By 6 months, affected roots showed a relative reduction in large myelinated fibers, increased proportions of small myelinated fibers and Schwann cell nuclei. Numbers of unmyelinated fibers were reduced relative to 3- to 6-week lesions. Axonal degeneration and regeneration appears to be the chief pathological change in sensory roots in this model. If regenerated fibers arise from latently infected neurons, then establishment of latency is not a relatively silent event, but is associated with major long-lasting, morphologically detectable effects.
authors.affiliation: Laboratory of Experimental Neuropathology, National Institutes of Health, Bethesda, MD 20892.
authors.forename: D
authors.initials: D
authors.lastname: Soffer
authors.forename: J R
authors.initials: JR
authors.lastname: Martin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006558:Herpes Genitalis / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009410:Nerve Degeneration; D009416:Nerve Regeneration; D009475:Neurons, Afferent / Q000473:pathology*; D013126:Spinal Nerve Roots / Q000473:pathology*