title: Micronucleus test with cyclophosphamide administered by intraperitoneal injection and oral gavage.
abstract: The effect of route of administration on the micronucleus test was examined in 2 laboratories: cyclophosphamide (CYP) was administered by intraperitoneal injection (i.p.) or oral gavage (p.o.) to 2 strains of mice. MS/Ae and CD-1. On the basis of a small-scale acute toxicity study and a pilot micronucleus experiment, the final micronucleus test was performed with a 48-h sampling time at doses of 25-200 mg/kg i.p. and 50-400 mg/kg p.o. CYP via the i.p. route was more toxic and induced more micronucleated polychromatic erythrocytes (MNPCEs) in MS/Ae mice than in CD-1 mice. Administration-route-related differences were not distinctly shown in the MS/Ae strain. In CD-1, however, higher doses were required for the p.o. route than for the i.p. route to induce about equal amounts of clastogenic damage.
authors.affiliation: Safety Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd., Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Wakata
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: M
authors.initials: M
authors.lastname: Tamaoki
authors.forename: T
authors.initials: T
authors.lastname: Ohshima
authors.forename: M
authors.initials: M
authors.lastname: Kojima
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003520:Cyclophosphamide / Q000008:administration & dosage* / Q000633:toxicity; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage*; D010865:Pilot Projects
chemical_list: D009153:Mutagens; D003520:Cyclophosphamide