The cloning and expression of transfer RNA gene cluster of Vibrio eltor phage e4.
Vibrio eltor phage e4 codes for five different tRNA species (S. Chattopadhyay and R. K. Ghosh, 1988, Virology, 165, 606-608). The tRNA genes contained in a 3.4-kb KpnI fragment (S. Chattopadhyay and R. K. Ghosh, 1988, Virology, 162, 337-345) have been cloned in pUC 19 at the KpnI site. Two recombinant plasmids, pSR216 and pSR112, produced four of the five tRNA species (arginine, isoleucine, tyrosine, and tryptophan) encoded in the phage genome. The tRNA genes were located on a 1.45-kb KpnI-HindIII subfragment.
Chattopadhyay
S
S
Indian Institute of Chemical Biology, Calcutta.
Ghosh
R K
RK
D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D010957:Plasmids; D012343:RNA, Transfer / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014733:Vibrio / Q000235:genetics*
D004279:DNA, Viral; D012367:RNA, Viral; D012343:RNA, Transfer