Differences in the influence of diet on hepatic glutathione S-transferase activity and glutathione content between young and old C57 black female mice.
The effect of a protein-free diet (PFD) on hepatic activity of glutathione S-transferase (GST) and hepatic content of total glutathione (GSH) was examined in young (9-month-old), middle-aged (17-month-old) and old (27-month-old) C57BL/6CrS1c female mice. There were no significant differences in the control values of GSH or of enzyme activity for four of five substrates among young, middle-aged, and old animals fed normal diet (ND) only. Both GSH and GST activity were significantly decreased by the 7-day PFD in both young and old groups but the decrement was generally greater in old mice. After a 2-3-day refeeding of ND, young mouse enzyme activities were significantly higher than control (basal) values for all five substrates, whereas old mouse values were still significantly lower than corresponding control values. There was no overshooting of GSH levels after refeeding of ND in either young or old animals. This study indicates that an age difference in this detoxification system can be clearly demonstrated in the hepatic response to PFD feeding and especially to ND refeeding, despite the enzymes' stable basal activities with aging.
Carrillo
M C
MC
First Laboratory of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Kitani
K
K
Kanai
S
S
Sato
Y
Y
Nokubo
M
M
Ohta
M
M
Otsubo
K
K
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002074:Butanones / Q000378:metabolism; D004044:Dietary Proteins / Q000008:administration & dosage; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004852:Epoxy Compounds / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000032:analysis*; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000032:analysis* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009578:Nitrobenzenes / Q000378:metabolism; D011488:Protein Deficiency / Q000378:metabolism* / Q000473:pathology; D011506:Proteins / Q000032:analysis; D013448:Sulfobromophthalein / Q000378:metabolism
D002074:Butanones; D004044:Dietary Proteins; D004137:Dinitrochlorobenzene; D004852:Epoxy Compounds; D009578:Nitrobenzenes; D011506:Proteins; D013448:Sulfobromophthalein; C028328:1,2-dichloro-4-nitrobenzene; C013690:styrene oxide; C025383:benzylideneacetone; D005982:Glutathione Transferase; D005978:Glutathione