Effect of methylazoxymethanol acetate on bluegill sunfish cell cultures in vitro.
An epithelioid cell line derived from fin tissue of bluegill sunfish (designated BG/F) exhibited early indications of cell transformation upon exposure to methylazoxymethanol acetate (MAM acetate). Such changes included the induction of polyploidy, increased colony-forming efficiency, loss of contact inhibition, and formation of transformed foci. Unlike later transformation characteristics observed with mammalian cells, the MAM acetate-treated BG/F cells could not be propagated under conditions of anchorage independence in soft agar. Incubation of BG/F cells with N-methyl-N'-nitro-N-nitrosoguanidine, followed by exposure to 12-O-tetradecanoylphorbol-13-acetate, was not observed to cause cell transformation under the experimental conditions. The controls of a fibroblastic cell culture derived from gill tissue of bluegill sunfish showed spontaneous transformation after 6 months of passage, similar to the transformation observed in the experimental MAM acetate treated gill cultures.
Borenfreund
E
E
Laboratory Animal Research Center, Rockefeller University, New York, New York 10021.
Babich
H
H
Martin-Alguacil
N
N
D000818:Animals; D001391:Azo Compounds / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002875:Chromosomes / Q000187:drug effects; D005399:Fishes; D005880:Gills / Q000166:cytology; D008746:Methylazoxymethanol Acetate / Q000633:toxicity*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D014873:Water Pollutants / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D001391:Azo Compounds; D002273:Carcinogens; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D008746:Methylazoxymethanol Acetate; D006899:Mixed Function Oxygenases