The response of liver to lactobionate/raffinose (University of Wisconsin--UW) solution during hypothermic preservation: a study using 31phosphorus nuclear magnetic resonance.
31P nuclear magnetic resonance spectroscopy has been used to study rat livers following flushing with the University of Wisconsin (UW) lactobionate/raffinose solution (N. Jamieson, R. Sundberg, S. Lindell, J. Southard, and F.O. Belzer, Cryobiology 24, 573-574, 1987; M. Kalayoglu, H. Sollinger, R. Stratta, A. D'Alessandro, R. Hoffman, J. Pirsch, and F. O. Belzer, Lancet 1, 617-619, 1988). These studies have revealed that despite the improved storage properties that have been reported for this solution, hepatic ATP and ADP declined at a rate similar to that seen in the more commonly used Marshall's or Collins' solutions. However, there was a significant inhibition of the developing acidosis, such that by 5 hr postflush, the intracellular pH was 7.17 +/- 0.06 (mean +/- SD, n = 5) compared to 6.90 +/- 0.06 for Marshall's solution (4 hr postflush) and 6.94 +/- 0.04 for Collins' solution (4 hr postflush). This did not appear to be due to a buffering effect of the solution, as this was found to be relatively low, but was probably due to a modification of hepatic metabolism caused by the solution itself.
Busza
A L
AL
Department of Physics in Relation to Surgery, Hunterian Institute, Royal College of Surgeons, Lincoln's Inn Fields, London, United Kingdom.
Fuller
B J
BJ
Proctor
E
E
D000818:Animals; D003451:Cryoprotective Agents; D004187:Disaccharides; D005615:Freezing; D008099:Liver / Q000378:metabolism / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009844:Oligosaccharides; D009926:Organ Preservation / Q000379:methods*; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains
D003451:Cryoprotective Agents; D004187:Disaccharides; D009844:Oligosaccharides; C005608:lactobionic acid; D011887:Raffinose