Alpha-L-fucosidase activity in normal human lymphocytes.
After DEAE-Trisacryl chromatography two forms of alpha-L-fucosidase have been characterized in normal human lymphocytes. These enzymatic forms were different with respect to their optimum pH, kinetic properties and isoelectric behaviour. After neuraminidase treatment two forms are still observed with a neutral shift in pI values. These results suggest that at least two structurally different alpha-L-fucosidase units exist.
Roger
L
L
Laboratoire de Chimie Biologique, U.E.R. de Biologie Humaine et Expérimentale, Université René Descartes, Paris, France.
Bernard
M A
MA
Percheron
F
F
Foglietti
M J
MJ
D000328:Adult; D002848:Chromatography, DEAE-Cellulose; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007525:Isoelectric Focusing; D008214:Lymphocytes / Q000201:enzymology*; D005644:alpha-L-Fucosidase / Q000378:metabolism*
D005644:alpha-L-Fucosidase