[Qualitative and quantitative changes of luteinizing hormone (LH) in the pituitary glands in female Japanese monkeys (Macaca fuscata) during the menstrual cycle and after castration].
The present study was designed to investigate qualitative and quantitative changes in luteinizing hormone (LH) in the pituitary glands of female Japanese monkeys (Macaca fuscata) during the menstrual cycle and after castration. The pituitary glands were removed from 10 intact cycling Japanese monkeys and 3 castrated ones: The former sacrificed in the early, late follicular phase, and mid luteal phase, and the latter 8 weeks after castration. All 13 pituitary glands were fractionated by isoelectrofocusing (IEF). The mid cycle peaks of estradiol (E2) and bioactive LH occurred simultaneously in 11 out of 13 monkeys. No animals showed luteal phase peaks in the plasma E2 concentration. Bioactive LH content (IU/mg wet weight) in the pituitary gland was significantly correlated with plasma E2 levels in the cycling monkeys (R2 = 0.974) and it was the highest in monkeys in the late follicular phase. Bioactive LH in the pituitary gland was separated into 6 distinct species in terms of pI values by IEF; A(9.31 +/- 0.19), B(8.88 +/- 0.17), C(8.36 +/- 0.17), D(7.92 +/- 0.18), E(7.32 +/- 0.16) and F(acidic LH). Among alkaline LH species, the most predominant ones were Fractions B, C and D in the early follicular phase, Fraction A followed by B in the late follicular phase and Fraction B followed by C in the mid luteal phase. The neutral and acidic LH (Fraction E and F) was increased more in the early follicular phase and in the castrates. In conclusion, LH species separated by IEF may correspond to the stage of molecular maturation of pituitary LH.(ABSTRACT TRUNCATED AT 250 WORDS)
Hamada
K
K
Department of Obstetrics and Gynecology, School of Medicine, Ehime University.
Suginami
H
H
Matsuura
S
S
D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D005498:Follicular Phase; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008183:Luteal Phase; D007986:Luteinizing Hormone / Q000097:blood / Q000145:classification / Q000378:metabolism*; D008251:Macaca; D008597:Menstrual Cycle; D009929:Organ Size; D010052:Ovariectomy; D010902:Pituitary Gland / Q000378:metabolism* / Q000473:pathology; D011374:Progesterone / Q000097:blood
D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone