title: Vascular cholinesterases and choline uptake in isolated rat forebrain microvessels: a possible link.
abstract: The two parameters of the active [methyl-3H]choline uptake into isolated rat forebrain microvessels, Km and Vmax, were determined for 1-, 3-, 10-, and 24-month-old Charles River male rats and compared with the activities of the enzymes choline acetyltransferase (ChAT), acetylcholinesterase (AChE), and butyrylcholinesterase (BuChE) in these microvessels over the same time course. The value of Km remained constant over the entire period, but that of Vmax increased from 8.5 +/- 1.0 to 80.6 +/- 16.4 nmol g-1 (mean +/- SEM) over the first 3 months of life. Over the same period, the increase in ChAT activity, from an initial value of 7.1 +/- 1.6 to 10.2 +/- 0.3 nmol g-1 min-1, was not proportional to that of choline uptake. Levels of BuChE activity (0.9-1.3 mumol g-1 min-1) were almost unchanged throughout the entire 24-month period, but those of AChE showed a steady and significant increase from 1 to 24 months, remaining relatively high at senescence (4.7 mumol g-1 min-1), when choline uptake had decreased to one-third of its optimal value. Selective inhibition of AChE with 1,5-bis(4-allyldimethylammonium-phenyl)pentan-3-one dibromide (0.5 microM) in unruptured capillaries from 3-month-old rats resulted in a decrease in Vmax of choline uptake from approximately 81 to 59 nmol g-1 min-1 or with 9-amino-1,2,3,4-tetrahydroacridine (10 microM) in capillaries from 2-month-old rats from approximately 30 to 15 nmol g-1 min-1. Selective inhibition of BuChE with tetraisopropyl pyrophosphoramide (100 microM) resulted in an increase in Vmax from approximately 81 to 96 nmol g-1 min-1. It is possible that the two vascular enzyme systems are coupled to a hypothetical endothelial choline transporter, but with an action opposite to each other.
authors.affiliation: Department of Physiology and Pharmacology, Tel Aviv University, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Shimon
authors.forename: Y
authors.initials: Y
authors.lastname: Egozi
authors.forename: Y
authors.initials: Y
authors.lastname: Kloog
authors.forename: M
authors.initials: M
authors.lastname: Sokolovsky
authors.forename: S
authors.initials: S
authors.lastname: Cohen
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D001808:Blood Vessels / Q000201:enzymology / Q000378:metabolism; D002794:Choline / Q000378:metabolism*; D002802:Cholinesterases / Q000378:metabolism*; D004027:Diencephalon / Q000098:blood supply / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008833:Microcirculation; D051381:Rats; D011919:Rats, Inbred Strains; D013687:Telencephalon / Q000098:blood supply / Q000378:metabolism*
chemical_list: D002802:Cholinesterases; D002794:Choline