Phorbol ester induces differential membrane-association of protein kinase C subspecies in human platelets.
Human platelets contained proteins which cross-reacted with antisera specific for brain protein kinase C-alpha and -beta. When platelets were incubated with 12-O-tetradecanoylphorbol-13-acetate there was a rapid accumulation of protein kinase C-alpha in the particulate fraction associated with a loss of this subspecies from the soluble fraction. No particulate accumulation or soluble loss of protein kinase C-beta could be detected when platelets were incubated with the phorbol ester.
Fournier
A
A
School of Biological Sciences, Flinders University, Bedford Park, South Australia.
Hardy
S J
SJ
Clark
K J
KJ
Murray
A W
AW
D001792:Blood Platelets / Q000201:enzymology*; D015153:Blotting, Western; D002451:Cell Compartmentation / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D011493:Protein Kinase C / Q000097:blood* / Q000145:classification; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate