Solid-phase sample preparation method for prostaglandins: integration of procedures for isolation and derivatization for gas chromatographic determination.
A procedure using a solid-phase support has been developed for the isolation and derivatization of prostaglandins from biological matrices. The styrene-divinylbenzene cross-linked copolymeric macroreticular resin, XAD-2, was used as an adsorbent for prostaglandin E2 from biological samples, as a support for the oximation of the carbonyl group and as a catalyst for pentafluorobenzylation. The reactor bed was then linked to a Florisil column for a final chromatographic clean-up. Matrix effects were found to affect the yield, but recovery of the desired electrophoric products was comparable with methods reported in the literature. The ease of sample preparation suggests that this technique may be a viable approach to automating the processes for preparing prostaglandins from biological matrices for gas chromatographic analysis.
Rosenfeld
J M
JM
Department of Pathology, Faculty of Health Sciences, McMaster University, Hamilton, Ontario, Canada.
Mureika-Russell
M
M
Love
M
M
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer; D015232:Dinoprostone / Q000302:isolation & purification*; D006801:Humans
D015232:Dinoprostone