Influence of cellular DNA content on survival in ovarian carcinoma.
Flow cytometric analysis of nuclear DNA content was performed on paraffin-embedded tissue from 153 ovarian epithelial carcinomas. The DNA ploidy, DNA index, and S phase fraction were determined without knowing the final outcome of the patients. Fifty-one (38%) of the 134 classifiable histograms were considered to be diploid, 70 (52%) aneuploid, and 13 (10%) multiploid. The DNA index was a better prognostic factor for survival than DNA ploidy; 48% of the patients with a small DNA index (less than 1.3) survived for 5 years after the diagnosis, in contrast to 14% of the patients with a large DNA index (greater than 1.3) (P less than .0001). Carcinomas with a small S phase fraction (less than 11%) were associated with favorable survival (P = .0002). In multivariate analysis, the size of the residual tumor at surgery (P less than .001), the DNA index (P = .001), and the S phase fraction (P = .05) were the only significant independent prognostic factors. The results indicate that flow cytometric nuclear DNA content analysis provides important additional information for the estimation of survival in ovarian carcinoma.
Klemi
P J
PJ
Department of Pathology, University Central Hospital of Turku, Finland.
Joensuu
H
H
Mäenpää
J
J
Kiilholma
P
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000032:analysis / Q000401:mortality* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000032:analysis / Q000401:mortality* / Q000473:pathology; D011003:Ploidies; D011379:Prognosis
D004273:DNA, Neoplasm