title: Regulation and role in pathogenicity of Erwinia chrysanthemi 3937 pectin methylesterase.
abstract: The gene pem, encoding the pectin methylesterase (PME) of Erwinia chrysanthemi 3937, was cloned and mutagenized by mini-Mu transposable elements. A second gene, pecY, which could act as a negative regulator of PME was found 5' to the pem gene. A PME-E. chrysanthemi derivative inoculate onto Saintpaulia plants was shown to be clearly noninvasive, demonstrating the important role of this enzyme in soft rot disease.
authors.affiliation: Laboratoire de Pathologie Végétale, Institut National Agronomique, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Boccara
authors.forename: V
authors.initials: V
authors.lastname: Chatain
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000502:physiology*; D004885:Erwinia / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D010935:Plant Diseases; D014774:Virulence
chemical_list: D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase