[Distribution of aminoacyl-t-RNA-synthetase activity in rabbit liver cells during disruption of protein biosynthesis in experimental myocardia infarct].
Distribution of the aminoacyl-tRNA synthetase activity has been studied in the normal rabbit liver cells and in the model of protein synthesis damage, i.e. under experimental myocardial infarction (EMI). The activity of a number of aminoacyl-tRNA synthetases in postmitochondrial and postribosomal extracts from rabbit liver homogenate has been determined to increase 12 h after EMI. Gel filtration of the postribosomal extract on Sepharose 6B shows that the activity of aminoacyl-tRNA synthetases is distributed among the fractions with Mr 1.82 x 10(6), 0.84 x 10(6) and 0.12 = 0.35 x 10(6). The first two fractions (high-molecular-weight aminoacyl-tRNA synthetase complexes) contain arginyl-, glutamyl-, isoleucyl-, leucyl-, lysyl- and valyl-tRNA synthetases, whereas the low-molecular-weight fraction contains alanyl-, arginyl-, glycyl-, phenylalanyl-, seryl-, threonyl-, tryptophanyl- and tyrosyl-tRNA synthetases. In a case of EMI all the aminoacyl-tRNA synthetases translocate from the complexes with Mr 1.82 x 10(6) into the complexes with Mr 0.84 x 10(6), what provided evidence for the possibility to regulate protein synthesis by changes in compartmentalization of aminoacyl-tRNA synthetases.
Ivanov
L L
LL
Martinkus
Z P
ZP
Lekis
A V
AV
Lukoshiavichius
L Iu
LIu
Prashkiavichius
A K
AK
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D009203:Myocardial Infarction / Q000201:enzymology* / Q000378:metabolism; D014176:Protein Biosynthesis; D011817:Rabbits
D000604:Amino Acyl-tRNA Synthetases
Raspredelenie aminoatsil-t-RNK-sintetaznoì aktivnosti v kletkakh pecheni krolikov pri narushenii biosinteza belka v usloviiakh éksperimental'nogo infarkta miokarda.