Increased ultra weak chemiluminescence emission from rat heart at postischemic reoxygenation: protective role of vitamin E.
Aim of this study was to confirm an increased free radical generation rate during ischemia-reoxygenation, by ultra-weak chemiluminescence detection at the surface of perfused rat heart. We observed that reoxygenation following 30 min global ischemia, induces an increase of ultraweak chemiluminescence emission in isolated perfused heart only if partial depletion of vitamin E is induced by dietary manipulation. Moreover, in normal diet fed rats, vitamin E is partially consumed during global ischemia, but not during reoxygenation. Since chemiluminescence increases during post-ischemic reperfusion, when vitamin E myocardial content is lowered, the most probable free radicals involved are the hydroperoxyl radical derivatives of lipids. These radicals, indeed, are known both to produce photoemission by disproportion and to react with vitamin E. On the other hand, the nature of the reaction that consumes vitamin E during ischemia is still obscure. Accordingly, the basal level of vitamin E myocardial content seems to be a key factor for protecting the heart against reoxygenation injury and its consumption during ischemia could be a determinant of myocardial sensitivity to oxidative stress during reperfusion.
Barsacchi
R
R
Department of Physiology and Biochemistry, University of Pisa, Italy.
Coassin
M
M
Maiorino
M
M
Pelosi
G
G
Simonelli
C
C
Ursini
F
F
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D003327:Coronary Disease / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D008163:Luminescent Measurements; D008297:Male; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014810:Vitamin E / Q000494:pharmacology; D014811:Vitamin E Deficiency / Q000378:metabolism*
D014810:Vitamin E; D001205:Ascorbic Acid