Reductive dehydroxylation of 4-hydroxybenzoyl-CoA to benzoyl-CoA in a denitrifying, phenol-degrading Pseudomonas species.
The initial reactions in anaerobic degradation of phenol to CO2 have been studied in vitro with a denitrifying Pseudomonas strain grown with phenol and nitrate in the absence of molecular oxygen. Phenol has been proposed to be carboxylated to 4-hydroxybenzoate [(1987) Arch. Microbiol. 148, 213-217]. 4-Hydroxybenzoate was activated to 4-hydroxybenzoyl-CoA by a coenzyme A ligase. Cell extracts also catalyzed the reductive dehydroxylation of 4-hydroxybenzoyl-CoA to benzoyl-CoA with reduced benzyl viologen as electron donor. This enzyme, benzoyl-CoA:(acceptor) 4-oxidoreductase (hydroxylating) (EC 1.3.99.-), has not been reported before. The data suggest that phenol and 4-hydroxybenzoate are anaerobically metabolized by this strain via benzoyl-CoA.
Glöckler
R
R
Abteilung Angewandte Mikrobiologie, University of Ulm, FRG.
Tschech
A
A
Fuchs
G
G
D000214:Acyl Coenzyme A / Q000378:metabolism*; D007700:Kinetics; D010088:Oxidoreductases / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010226:Parabens / Q000378:metabolism; D019800:Phenol; D010636:Phenols / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology*
D000214:Acyl Coenzyme A; D010226:Parabens; D010636:Phenols; C058482:4-hydroxybenzoyl-coenzyme A; D019800:Phenol; C037817:benzoyl-coenzyme A; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C060482:benzoyl-coenzyme A-4-oxidoreductase; C038193:4-hydroxybenzoic acid