title: Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction.
abstract: Enzymatic DNA amplification was applied to DNA and elementary bodies of C. trachomatis. Oligonucleotide primers were chosen in a sequence of a conserved domain of the major outer membrane protein to generate the amplification of a 129-base pair fragment. This sequence was amplified in the 15 serovars of C. trachomatis; however, serovar J gave a weaker signal than the others. The specificity was controlled by EcoRI restriction enzyme digestion and Southern analysis using an internal probe of the amplified sequence. No cross-reaction was shown with DNA of 11 other bacteria. Thus, enzymatic DNA amplification by the polymerase chain reaction appears to be a potential tool for the specific detection of C. trachomatis.
authors.affiliation: Laboratoire de Bactériologie, Hôpital Pellegrin, Bordeaux, France.
authors.forename: B
authors.initials: B
authors.lastname: Dutilh
authors.forename: C
authors.initials: C
authors.lastname: Bébéar
authors.forename: P
authors.initials: P
authors.lastname: Rodriguez
authors.forename: A
authors.initials: A
authors.lastname: Vekris
authors.forename: J
authors.initials: J
authors.lastname: Bonnet
authors.forename: M
authors.initials: M
authors.lastname: Garret
mesh_terms: D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D005784:Gene Amplification; D015345:Oligonucleotide Probes; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D015246:Deoxyribonuclease EcoRI