Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever.
A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.
Rubin
F A
FA
Department of Bacterial Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
McWhirter
P D
PD
Punjabi
N H
NH
Lane
E
E
Sudarmono
P
P
Pulungsih
S P
SP
Lesmana
M
M
Kumala
S
S
Kopecko
D J
DJ
Hoffman
S L
SL
D001345:Autoradiography; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*; D018805:Sepsis / Q000175:diagnosis / Q000382:microbiology*; D014435:Typhoid Fever / Q000175:diagnosis / Q000382:microbiology*
D015342:DNA Probes; D004269:DNA, Bacterial