[Measurement of the concentration of free cytoplasmic calcium in the process of platelet aggregation using a fluorescent method].
A multiwavelength method for measuring free cytosolic calcium concentration is proposed. It is based on the registration of the fluorescent spectrum of calcium--sensitive probe indo-1 and deconvolution of the spectrum into components corresponding to free and bound forms of the probe. Calcium concentration is calculated as a product of calcium-probe dissociation constant by calcium-bound to free form concentration ratio. The obtained values are independent of variations in light-scattering properties of the medium and total dye concentration in the optical channel. It is shown that during ADP-induced platelet aggregation calcium concentration rises without measurable delay after the addition of the inducer and significantly decreases by the time the aggregation begins.
Popov
E G
EG
Gavrilov
I Iu
IIu
Pozin
E Ia
EIa
Gabbasov
Z A
ZA
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D005456:Fluorescent Dyes; D008027:Light; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D000244:Adenosine Diphosphate; D002118:Calcium
Izmerenie kontsentratsii svobodnogo tsitoplazmaticheskogo kal'tsiia v protsesse agregatsii trombotsitov fluorestsentnym metodom.