title: Increased sinusoidal volume and solute extraction during retrograde liver perfusion.
abstract: Retrograde isolated liver perfusion has been used to probe acinar functional heterogeneity, but the hemodynamic effects of backward flow have not been characterized. In this study, extraction of a long-chain fatty acid derivative, 12-N-methyl-7-nitrobenzo-2-oxa-1,3-diazol-amino stearate (12-NBDS), was greater during retrograde than during anterograde perfusion of isolated rat liver. To determine whether hemodynamic differences between anterograde and retrograde perfused livers could account for this finding, the hepatic extracellular space was measured for both directions of flow by means of [14C]sucrose washout during perfusion as well as by direct measurement of [14C]sucrose entrapped during perfusion. A three- to fourfold enlargement of the total hepatic extracellular space was found during retrograde perfusion by both approaches. Examination of perfusion-fixed livers by light microscopy and morphometry revealed that marked distension of the sinusoids occurred during retrograde perfusion and that this accounts for the observed increase in the [14C]sucrose space. These findings support the hypothesis that maximum resistance to perfusate flow in the isolated perfused rat liver is located at the presinusoidal level. In addition, increased transit time of perfusate through the liver and greater sinusoidal surface area resulting from sinusoidal distension may account for the higher extraction of 12-NBDS and possibly other compounds by retrograde perfused liver.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143.
authors.forename: N M
authors.initials: NM
authors.lastname: Bass
authors.forename: J A
authors.initials: JA
authors.lastname: Manning
authors.forename: R A
authors.initials: RA
authors.lastname: Weisiger
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000502:physiology*; D008102:Liver Circulation; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013228:Stearates / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D013228:Stearates; C060027:12-N-methyl-7-nitrobenzo-2-oxa-1,3-diazolamino stearate; D013395:Sucrose; D009327:4-Chloro-7-nitrobenzofurazan