Synthesis of platelet activating factor by tissues from the rainbow trout, Salmo gairdneri.
In mammals, platelet activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a lipid mediator with biological activity at concentrations in the subnanomolar range. Although PAF is known to have many activities in mammals, little is known about its synthesis and importance in other vertebrate groups. We demonstrate here the synthesis of PAF from [3H]acetate by slices of trout gill, kidney, liver and spleen. PAF synthesis was stimulated by the calcium ionophore A23187 and was time-dependent. The radiolabeled PAF produced was characterized by TLC, HPLC, derivatization and by saponification and phospholipase A2 hydrolysis. These findings suggest that PAF may be an important mediator in fish.
Turner
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MR
Mountain Aquaculture Research Center, Western Carolina University, Cullowhee, NC.
Lumb
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RH
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005880:Gills / Q000378:metabolism; D006868:Hydrolysis; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010972:Platelet Activating Factor / Q000096:biosynthesis* / Q000378:metabolism; D012487:Salmonidae / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D014316:Tritium; D014337:Trout / Q000378:metabolism*
D010972:Platelet Activating Factor; D014316:Tritium