Detecting inhibition of coxsackievirus replication by measuring DNA synthesis--effect of proteinase inhibitors.
The replication of picornaviruses can be monitored by microscopic examination of the cytopathogenic effect. We found that measuring cellular DNA synthesis gave a more objective and reliable estimate of the viral effect on the cells. Furthermore, by simultaneously measuring DNA synthesis in uninfected cells, we obtained a sensitive indication of whether the agents tested influenced cellular activity. The method was employed to investigate the effect of various proteinase inhibitors on the replication of coxsackie-B3 virus in HEp2 cells. Certain inhibitors of metallo-proteinases had a limited, but consistent, protective effect against viral activity.
Grinde
B
B
Department of Virology, National Institute of Public Health, Oslo, Norway.
Flugsrud
L
L
Tjøtta
E
E
D000900:Anti-Bacterial Agents; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004770:Enterovirus / Q000254:growth & development*; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D009842:Oligopeptides / Q000494:pharmacology; D010455:Peptides; D011480:Protease Inhibitors / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D006146:Guanidines; D009842:Oligopeptides; D010455:Peptides; D011480:Protease Inhibitors; C016977:amastatin; D004247:DNA; C041445:diprotin A; D008666:Metalloendopeptidases; D019791:Guanidine