title: The isolation and partial characterization of chymotrypsinogen from the pancreas of the ostrich (Struthio camelus).
abstract: 1. Cationic chymotrypsinogen from the pancreas of the ostrich was purified to homogeneity by sulfuric acid extraction of pancrei, (NH4)2SO4 fractionation and SP-Sephadex C-50 and Sephadex G-100 chromatography. 2. The final preparation was homogeneous when subjected to SDS-PAGE, isoelectric focusing and sedimentation equilibrium centrifugation. The Mmin value obtained from amino acid analysis was 25,572 Da. A mean sedimentation coefficient of 2.575 S was obtained by sedimentation velocity centrifugation. 3. N-terminal analysis by dansylation showed an Ala residue which is the N-terminal of a neochymotrypsinogen. 4. The effects of pH, temperature and inhibitors (LBTI, PMSF, TPCK and DFP) on the chymotryptic activity were examined. A Km-value for ATEE as substrate was found to be 0.57 mM.
authors.affiliation: Department of Biochemistry, University of Port Elizabeth, Republic of South Africa.
authors.forename: N
authors.initials: N
authors.lastname: van der Westhuizen
authors.forename: R J
authors.initials: RJ
authors.lastname: Naudé
authors.forename: W
authors.initials: W
authors.lastname: Oelofsen
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001717:Birds / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D002919:Chymotrypsinogen / Q000302:isolation & purification* / Q000378:metabolism; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D013696:Temperature
chemical_list: D000596:Amino Acids; D002919:Chymotrypsinogen