Protein kinase C phosphorylates the sponge aggregation receptor after its binding to the homologous aggregation factor.
The aggregation factor from the sponge Geodia cydonium functions also as a growth factor after binding to the aggregation receptor (= growth factor receptor) on the plasma membrane of homologous cells. We have recently shown that protein kinase C is involved in the pathway transducing the growth factor signal. Here we report that the aggregation receptor (a polypeptide with an Mr of 43,500) is phosphorylated by protein kinase C. Using a plasma membrane fraction only this phosphoprotein (pp) 43.5 became phosphorylated by kinase C. The phosphorylation of pp43.5 in intact cells in response to the binding of the aggregation factor to this polypeptide was a late event and occurred 10 to 15 h after addition of the aggregation factor. Based on studies with phorbol esters it appears to be very likely that protein kinase C also phosphorylates pp43.5 in vitro. The degree of phosphorylation of pp43.5 paralleled with both the extent of DNA synthesis and ras oncogene expression. The latter process resulted in a switch of the responsiveness of the cells to growth factors signals: 10 to 15 h after addition of the aggregation factor to dissociated cells, this factor lost its growth factor function while the homologous lectin gained the ability to stimulate cell proliferation (to be published). These results support the idea that phosphorylation of pp43.5 (= aggregation receptor) results in an inhibition of its function, i.e., the transduction of the growth factor (= aggregation factor) signal.
Schröder
H C
HC
Institut für Physiologische Chemie, Universität, Mainz/Bundesrepublik Deutschland.
Amberger
V
V
Renneisen
K
K
Bachmann
M
M
Kurelec
B
B
Uhlenbruck
G
G
Müller
W E
WE
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D015815:Cell Adhesion Molecules; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011161:Porifera / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D011940:Receptor Aggregation / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000596:Amino Acids; D015815:Cell Adhesion Molecules; D010750:Phosphoproteins; D011506:Proteins; C013065:cell aggregation factors; D011493:Protein Kinase C; D013936:Thymidine