The in vitro unscheduled DNA synthesis (UDS) assay in rat primary hepatocytes: evaluation of 2-furoic acid and 7 drug candidates.
The in vitro unscheduled DNA synthesis assay (UDS) is part of the routine genetic toxicology screening at The Upjohn Company. The purpose of this paper is to report results for 8 compounds which were tested in the in-house genetic toxicology program. These compounds represent diverse chemical structure and most of them entered the screening program because they are biologically active in efficacy screens. All tests were carried out under Good Laboratory Practices Regulations of the U.S. Food and Drug Administration. None of the materials reported here produced an increase in UDS and therefore the UDS results with these compounds do not suggest potential for genotoxicity.
Aaron
C S
CS
Genetic Toxicology Research, Upjohn Company, Kalamazoo, MI 49001.
Harbach
P R
PR
Wiser
S K
SK
Grzegorczyk
C R
CR
Smith
A L
AL
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D005663:Furans / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains
D005663:Furans; D009153:Mutagens; C060089:2-furoic acid