title: Identification of radioactivity in cell nuclei from brain, pituitary gland and genital tract of male rhesus monkeys after the administration of [3H]testosterone.
abstract: Enzymes are present in the primate brain that convert testosterone into 17 beta-hydroxy-5 alpha-androstan-3-one (dihydrotestosterone), estradiol-17 beta and 4-androstene-3,17-dione. To identify the metabolites of testosterone that accumulate in cell nuclei obtained from different regions of the brain, 9 adult castrated male rhesus monkeys were injected with 5 mCi [3H]testosterone as an intravenous bolus. After 1 h, brains were rapidly removed and the left halves were used for autoradiography while the right halves were dissected to provide 14 samples. Radioactive metabolites in cell nuclei were identified by high-performance liquid chromatography (HPLC) and by repeated recrystallization. In autoradiograms of brain, most of the labeled neurons were in the hypothalamus, preoptic area and amygdala. These three regions also had the highest levels of radioactivity. The major form of this radioactivity was [3H]estradiol-17 beta (Type I tissues) and the major radioactive androgen present was [3H]testosterone. In all other brain regions and pituitary gland, the major form of radioactivity was unchanged [3H]testosterone (Type II tissues). In genital tract structures, [3H]dihydrotestosterone predominated (Type III tissues). These results suggested that, in contrast to its actions on genital tract structures, testosterone acts on neuronal nuclei mainly in unmetabolized form or after conversion to estradiol-17 beta.
authors.affiliation: Department of Psychiatry, Emory University School of Medicine, Atlanta, GA 30322.
authors.forename: R W
authors.initials: RW
authors.lastname: Bonsall
authors.forename: H D
authors.initials: HD
authors.lastname: Rees
authors.forename: R P
authors.initials: RP
authors.lastname: Michael
mesh_terms: D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D007700:Kinetics; D008253:Macaca mulatta; D008297:Male; D009919:Orchiectomy; D009928:Organ Specificity; D010902:Pituitary Gland / Q000378:metabolism; D012669:Seminal Vesicles / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*; D014316:Tritium
chemical_list: D014316:Tritium; D013739:Testosterone