Effect of dietary omega-3 and omega-6 fatty acids on development of azaserine-induced preneoplastic lesions in rat pancreas.
We examined the effect of varying the ratio of dietary omega-3 (omega 3) to omega-6 (omega 6) on the development of pancreatic preneoplastic lesions in male Wistar rats given azaserine at 14 days of age. As the ratio of dietary omega 3 to omega 6 fatty acids increased in a diet totaling 20% by weight of fat, the development of preneoplastic atypical acinar cell nodules (AACNs) at 4 months after dosing with azaserine decreased significantly. In addition, serum levels of prostaglandin thromboxane B2, prostaglandin E2, and 6-keto-prostaglandin F1 alpha decreased significantly. The fatty acid composition of the rbc membrane was also significantly influenced by the ratio of dietary omega 3 to omega 6 fatty acids. In a second experiment, we examined the effect of dietary intervention with a different type of fat (corn oil or menhaden oil) 2 months into the 4-month postdosing period on AACN development at the end of the post-dosing period. Intervention of the omega 6 fatty acid-rich diet with the omega 3 fatty acid-rich diet significantly decreased focal development. The opposite was true when intervention involved substituting the omega 3 fatty acid-rich diet with the omega 6 fatty acid-rich diet.
O'Connor
T P
TP
Division of Nutritional Sciences, Cornell University, Ithaca, NY.
Roebuck
B D
BD
Peterson
F J
FJ
Lokesh
B
B
Kinsella
J E
JE
Campbell
T C
TC
D000818:Animals; D001377:Azaserine / Q000009:adverse effects*; D002462:Cell Membrane / Q000032:analysis; D003314:Corn Oil / Q000009:adverse effects; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000009:adverse effects; D008297:Male; D010190:Pancreatic Neoplasms / Q000139:chemically induced*; D011230:Precancerous Conditions / Q000139:chemically induced*; D051381:Rats
D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; C033266:Menhaden oil; D003314:Corn Oil; D001377:Azaserine