Phosphoenolpyruvate carboxylase in lupin nodules and roots. Identification of the enzymatic forms.
Phosphoenolpyruvate carboxylase (PEPC) EC 4.1.1.31 was extracted from nodules and roots of 2-day-old seedlings of lupin (Lupinus luteus L.). Chromatography on DEAE-cellulose of the nodule extract gave two forms of the enzyme: PEPC I and PEPC II eluted at 0.3-0.35 M and 0.41-0.53 M Tris buffer, respectively. A third form PEPC III from lupin roots was eluted from DEAE-cellulose column at the same buffer concentration as PEPC II from nodules. PEPC I and PEPC II eluted at 0.3-0.35 M and 0.41-0.53 M Tris buffer, more active in the 6-week-old nodules binding effectively nitrogen than in the 12-week-old ones.
Marczewski
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Carbon and Nitrogen Metabolism Laboratory, A. Mickiewicz University, Poznań, Poland.
D002262:Carboxy-Lyases / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D007887:Fabaceae / Q000201:enzymology* / Q000382:microbiology; D007527:Isoenzymes / Q000302:isolation & purification; D009586:Nitrogen Fixation; D010730:Phosphoenolpyruvate Carboxylase / Q000302:isolation & purification*; D010946:Plants, Medicinal
D007527:Isoenzymes; D002262:Carboxy-Lyases; D010730:Phosphoenolpyruvate Carboxylase