Blood risk factor metabolites associated with heart disease and myocardial fatty acids in copper-deficient male and female rats.
Intact and castrated males and intact and ovariectomized female rats were fed a copper-deficient diet in order to establish whether the protection provided in females against cardiovascular pathology and mortality is due to endogenous sex hormones, and different levels of blood lipids and/or myocardial fatty acids. Seventy-three male and female rats were assigned to a copper-deficient diet (0.6 micrograms of copper/g diet) containing 62% fructose for 8 weeks. Twelve of the male rats underwent castration and 12 of the females were ovariectomized. All animals exhibited high levels of plasma cholesterol, triglycerides, and uric acid, which were neither affected by the sex of the rat nor by the surgical treatment. The composition of fatty acids of the myocardium was similar in males and females. Except for those animals that were sacrificed by us, all other male rats died of heart pathology. In contrast, none of the female rats exhibited heart pathology and none died of the deficiency. It is suggested that heart pathology and mortality in copper deficiency are sex related and not due to high levels of plasma cholesterol, triglycerides, and uric acid or to differences in myocardial fatty acid composition.
Fields
M
M
Division of Endocrinology, Georgetown University Medical School, Washington, DC 20007.
Lewis
C
C
Beal
T
T
Berlin
E
E
Kliman
P G
PG
Peters
R C
RC
D000818:Animals; D002369:Castration; D003300:Copper / Q000172:deficiency*; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D006331:Heart Diseases / Q000209:etiology*; D008055:Lipids / Q000097:blood; D008297:Male; D009206:Myocardium / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012307:Risk Factors; D012737:Sex Factors
D005227:Fatty Acids; D008055:Lipids; D003300:Copper