[Detection of Toxoplasma antibodies using the c-ELISA method].
Sensitive and high capacity ELISA techniques introduced for the detection of toxoplasmatic antibodies so far do not correlate very well with standard serological examination systems used in common diagnosis of Toxoplasmosis. The most frequently used method in the CSSR - SFT using tween-ether soluble antigen extract, and displaying very satisfactory agreement with the Sabin-Feldman test, was compared with the system of direct ELISA, using the same antigen extract and the described c-ELISA method. The latter method uses for antibody detection whole non-disintegrated formol-treated trophozoites of Toxoplasma gondii and thus in the reaction in particular membrane antigens, incl. latent ones, are involved. Parallel examination of 160 sera of subjects with clinical suspicion of toxoplasma infection by the standard CFT, method and methods ELISA and c-ELISA revealed a better correlation between CFT and c-ELISA (r = 0.8031) than between CFT and ELISA with soluble antigen (r = 0.6016). Other positive specific features of the c-ELISA method are the use of commercially produced antigen for the immunofluorescence test, considerable elimination of selective adsorption of the polystyrene carrier and the perspective of the long-term preservation of plates with the fixed antigen.
Sýkora
J
J
Pokorný
J
J
Zástĕra
M
M
Frühbauer
Z
Z
Svandová
E
E
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D008722:Methods; D014122:Toxoplasma / Q000276:immunology*
D000913:Antibodies, Protozoan
Detekce toxoplazmických protilátek metodou c-ELISA.