title: An automated high-performance liquid chromatographic trace enrichment method for the determination of metoclopramide in serum and its application to a bioequivalence human volunteer study.
abstract: An automated trace enrichment method for metoclopramide is described. Serum was injected on to a short column packed with PLRP-S 100A (a polymeric reversed-phase material). The unwanted components were washed off with borate buffer (pH 9.8) before switching to a Spherisorb ODS column for the separation. The limit of detection in serum samples was 2 ng/ml. The method was used in a pharmacokinetic study to compare the biovailability of two formulations of the drug.
authors.affiliation: Department of Clinical Chemistry, Rorer Health Care Limited, Eastbourne, East Sussex.
authors.forename: A P
authors.initials: AP
authors.lastname: Fairhead
authors.forename: S G
authors.initials: SG
authors.lastname: Brooks
authors.forename: K R
authors.initials: KR
authors.lastname: Butterworth
authors.forename: B A
authors.initials: BA
authors.lastname: Mangham
mesh_terms: D000328:Adult; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008787:Metoclopramide / Q000097:blood* / Q000493:pharmacokinetics; D011237:Predictive Value of Tests
chemical_list: D008787:Metoclopramide