Detection of IgG4 antibodies by immunoblotting in patients on vespid venom immunotherapy.
A nitrocellulose immunoblotting procedure has been used to monitor patient specific IgG antibodies during Hymenoptera vespid venom therapy. By using monoclonal antibodies in the immunoblot assay the relative IgG4 antibody levels could be analysed semiquantitatively. Treatment with vespid venom over 2 years resulted in rises in venom-specific IgG4 antibodies mostly directed towards antigen 5, phospholipase A and hyaluronidase. The use of high quality monoclonal antibodies in immunoblotting assays offers an improvement in the in vitro evaluation of venom immunotherapy.
Einarsson
R
R
Pharmacia Diagnostics AB, Uppsala, Sweden.
Inganäs
M
M
Karlsson
T
T
Persson
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D000997:Antivenins / Q000032:analysis*; D001514:Bee Venoms / Q000627:therapeutic use*; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000276:immunology; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis*; D007167:Immunotherapy; D010740:Phospholipases / Q000276:immunology; D014862:Wasp Venoms / Q000276:immunology / Q000627:therapeutic use*; D014863:Wasps / Q000276:immunology
D000911:Antibodies, Monoclonal; D000997:Antivenins; D001514:Bee Venoms; D007074:Immunoglobulin G; D014862:Wasp Venoms; D010740:Phospholipases; D006821:Hyaluronoglucosaminidase