title: Palmitic and erucic acid metabolism in isolated perfused hearts from weanling pigs.
abstract: Hearts from 4 week-old weanling pigs were capable of continuous work output when perfused with Krebs-Henseleit buffer containing 11 mM glucose. Perfused hearts metabolized either glucose or fatty acids, but optimum work output was achieved by a combination of glucose plus physiological concentrations (0.1 mM) of either palmitate or erucate. Higher concentrations of free fatty acids increased their rate of oxidation but also resulted in a large accumulation of neutral lipids in the myocardium, as well as a tendency to increased acetylation and acylation of coenzyme A and carnitine. When hearts were perfused with 1 mM fatty acids, the work output declined below control values. Erucic acid is known to be poorly oxidized by isolated rat heart mitochondria and, to a lesser degree, by perfused rat hearts. In addition, it has been reported that erucic acid acts as an uncoupler of oxidative phosphorylation. In isolated perfused pig hearts used in the present study, erucic acid oxidation rates were as high as palmitate oxidation rates. When energy coupling was measured by 31P-NMR, the steady-state levels of ATP and phosphocreatine during erucic acid perfusion did not change noticeably from those during glucose perfusion. It was concluded that the severe decrease in oxidation rates and ATP production resulting from the exposure of isolated pig and heart mitochondria to erucic acid are not replicated in the intact pig heart.
authors.affiliation: Animal Research Centre, Research Branch, Central Experimental Farm, Ottawa, Canada.
authors.forename: F D
authors.initials: FD
authors.lastname: Sauer
authors.forename: J K
authors.initials: JK
authors.lastname: Kramer
authors.forename: G V
authors.initials: GV
authors.lastname: Forester
authors.forename: K W
authors.initials: KW
authors.lastname: Butler
mesh_terms: D000107:Acetylation; D000215:Acylation; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002331:Carnitine / Q000378:metabolism; D003065:Coenzyme A / Q000378:metabolism; D004734:Energy Metabolism; D004883:Erucic Acids / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D013552:Swine; D014886:Weaning
chemical_list: D004883:Erucic Acids; D005229:Fatty Acids, Monounsaturated; D010169:Palmitic Acids; D010743:Phospholipids; D010725:Phosphocreatine; C049811:erucic acid; D019308:Palmitic Acid; D000255:Adenosine Triphosphate; D005947:Glucose; D002331:Carnitine; D003065:Coenzyme A