The BCR-ABL oncogene transforms Rat-1 cells and cooperates with v-myc.
The tyrosine kinase P210 is the gene product of the rearranged BCR-ABL locus on the Philadelphia chromosome (Ph1), which is found in leukemic cells of patients with chronic myelogenous leukemia. It has a weakly oncogenic effect in immature murine hematopoietic cells and does not transform NIH 3T3 cells. We have found that P210 has a strikingly different effect in Rat-1 cells, another line of established rodent fibroblasts. Stable expression of P210 in Rat-1 cells caused a distinct morphological change and conferred both tumorigenicity and capacity for anchorage-independent growth. The introduction of v-myc into Rat-1 cells expressing P210 led to complete morphological transformation and enhanced tumorigenicity. No such interaction took place in NIH 3T3 cells. Thus, Rat-1 cells can be used to detect cooperation between BCR-ABL and other oncogenes and may prove useful for the identification of secondary oncogenic events in chronic myelogenous leukemia.
Lugo
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Department of Microbiology, University of California, Los Angeles 90024-1570.
Witte
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D000011:Abelson murine leukemia virus / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006801:Humans; D007942:Leukemia, Experimental / Q000235:genetics / Q000473:pathology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics; D009857:Oncogenes; D051381:Rats
D009856:Oncogene Proteins, Viral