Biotransformation of beta-nortestosterone by cultured porcine hepatocytes.
The metabolism of beta-nortestosterone by porcine hepatocytes was investigated. Initially beta-nortestosterone was rapidly oxidized to norandrostenedione, which was further transformed into a number of more hydrophilic compounds. It is assumed that most of these compounds were glucuronides, considering the effect of beta-glucuronidase treatment. The main product of enzymatic cleavage was investigated by gas chromatography-mass spectrometry but could not be identified until now.
Hoogenboom
L A
LA
State Institute for Quality Control of Agricultural Products (RIKILT), Wageningen, The Netherlands.
Berghmans
M C
MC
Traag
W A
WA
D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000378:metabolism*; D009277:Nandrolone / Q000493:pharmacokinetics*; D013552:Swine
D009277:Nandrolone