title: Solid phase synthesis of oligoribonucleotides using the 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) group for the protection of the 2'-hydroxy functions and the H-phosphonate approach.
abstract: The solid phase synthesis of oligoribonucleotides using the H-phosphonate approach and the 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) and dimethoxytrityl (DMTr) groups, respectively, for the protection of the 2'- and 5'-hydroxy functions is described. The use of a new reagent, tris-(1,1,1,3,3,3-hexafluoro-2-propyl) phosphite for the preparation of nucleoside H-phosphonate units is also discussed in detail.
authors.affiliation: Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Sakatsume
authors.forename: M
authors.initials: M
authors.lastname: Ohtsuki
authors.forename: H
authors.initials: H
authors.lastname: Takaku
authors.forename: C B
authors.initials: CB
authors.lastname: Reese
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates; D010758:Phosphorus; D010880:Piperidines
chemical_list: D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D063065:Organophosphonates; D010880:Piperidines; D010758:Phosphorus