Administration of recombinant human insulin-like growth factor I to pigs: determination of circulating half-lives and chromatographic profiles.
Recombinant human insulin-like growth factor I (IGF-I) was injected daily (4 or 8 mg/pig) as an intra-arterial bolus into pigs for 3 consecutive days and the serum IGF-I concentration was measured to determine disappearance profiles. IGF-I partitioned to a fast component (half-life, t 1/2 = 5.7 min) and a slow component (t 1/2 = 253 min) in pig serum. Chromatography of serum revealed that the fast component comprised unbound IGF-I, whereas the slow component comprised IGF-I bound to 40- and 150-kD serum IGF-binding proteins. In addition, administration of exogenous IGF-I caused significant hypoglycemia.
Walton
P E
PE
Department of Dairy and Animal Science, Pennsylvania State University, University Park.
Gopinath
R
R
Burleigh
B D
BD
Etherton
T D
TD
D000818:Animals; D002845:Chromatography; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000097:blood*; D008297:Male; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000097:blood; D013002:Somatomedins / Q000097:blood*; D013552:Swine / Q000097:blood*
D011994:Recombinant Proteins; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I