Cell surface of Micrococcus luteus: chemical treatment of the cells and teichuronic acids on the surface.
Micrococcus luteus IFO 3333 cells, both treated with chemical reagents and non-treated, were observed with a scanning electron microscope (SEM). The agglutinability of the cells with antiserum containing anti-teichuronic acid antibody was examined. The binding of protein A-gold particles to the cells, mediated with the antiserum, was also observed with SEM. The surface of a M. luteus cell consisted of two or three areas with borders--the rough and the smooth areas, or the rough, the slightly rough, and the smooth areas; fluffy materials were clearly seen in the rough area. Gold particles were observed uniformly and densely on the whole cell surface. However, either mild acid treatment or mild Smith degradation of the cells altered the fluffy rough area to a rough one, and extremely decreased the agglutinability and the binding of protein A-gold particles. Teichuronic acids appeared to be distributed uniformly on the whole cell surface of M. luteus IFO 3333.
Monodane
T
T
Department of Microbiology, Faculty of Dentistry, Kagoshima University.
Kusamichi
M
M
Tokunaga
M
M
Torii
M
M
D000372:Agglutination Tests; D002473:Cell Wall / Q000032:analysis / Q000648:ultrastructure; D006046:Gold; D006601:Hexoses / Q000032:analysis; D006851:Hydrochloric Acid; D008837:Micrococcus / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D014574:Uronic Acids / Q000032:analysis*
D006601:Hexoses; D011135:Polysaccharides, Bacterial; D014574:Uronic Acids; C010788:teichuronic acid; D006046:Gold; D006851:Hydrochloric Acid