Quantification of rat T-kininogen using immunological methods. Application to inflammatory processes.
Antibodies raised in rabbits against rat T-kininogen (alpha 1-cysteine proteinase inhibitor) were used to develop a radioimmunoassay and a nephelometric quantification for T-kininogen. These assays were specific and analytically reliable. We also described a radioimmunoassay for kinin measurement. These immunological methods have been used to study the behaviour of T-kininogen during inflammatory processes and specify the two properties of this kind of kininogen: its inhibitory capacity towards cysteine proteinases and its activity as precursor of T-kinin. Control plasma level of T-kininogen in male rats was lower than that of female rats. The maximum level was observed in plasma, liver, kidney and uterus of female rats during metestrus. After turpentine injection, T-kininogen level increased not only in plasma but also in liver and kidney. In carrageenan-induced peritoneal exudates, we found a large accumulation of T-kininogen and of immunoreactive kinins, these latter being identified by HPLC as bradykinin.
Adam
A
A
Faculté de Pharmacie, Université de Montréal, Canada.
Damas
J
J
Calay
G
G
Renard
C
C
Remacle-Volon
G
G
Bourdon
V
V
D000818:Animals; D005260:Female; D007249:Inflammation / Q000378:metabolism*; D007704:Kininogens / Q000032:analysis* / Q000276:immunology; D007705:Kinins / Q000032:analysis; D008297:Male; D009391:Nephelometry and Turbidimetry; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D014425:Turpentine / Q000494:pharmacology
D007704:Kininogens; D007705:Kinins; D014425:Turpentine