title: Interaction of the novel agent amphethinile with tubulin.
abstract: The novel agent amphethinile is shown to inhibit tubulin assembly in vitro. This agent is capable of displacing colchicine but not vinblastine from tubulin and causes a stimulation in GTPase activity in vitro. The affinity constant for the association of this drug with tubulin has been determined (Ka = 1.3 x 10(6) M-1). It is concluded that amphethinile belongs to the class of agents which share a common binding site with colchicine on the tubulin molecule.
authors.affiliation: Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
authors.forename: A T
authors.initials: AT
authors.lastname: McGown
authors.forename: B W
authors.initials: BW
authors.lastname: Fox
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002417:Cattle; D003078:Colchicine / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D007211:Indoles; D014404:Tubulin; C055673:amphethinile; D003078:Colchicine