Diagnostic screening of multiple antigen-antibody reactions in a new single assay on nitrocellulose: the line immunobinding assay (LIBA).
A new nitrocellulose-based ELISA technique, the line immunobinding assay (LIBA), permits the quantitative detection of multiple antigen-antibody reactions in one single assay. The antigen solutions are sprayed in thin lines onto a nitrocellulose sheet which is then cut into several strips before incubation in a multi-channel tray with serially diluted serum. The reacting antibodies are visualized by a peroxidase system. Comparison with other techniques routinely used for the detection of antibodies against measles, mumps and rubella, suggest that the LIBA offers specificity with sensitivity and may have a broad range of applications.
Aeppli
R E
RE
Institute for Immunology and Virology, University of Zurich, Switzerland.
Bargetzi
M J
MJ
Binz
H
H
D000914:Antibodies, Viral / Q000032:analysis*; D003101:Collodion; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008459:Measles virus / Q000276:immunology; D009109:Mumps virus / Q000276:immunology; D012412:Rubella virus / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*
D000914:Antibodies, Viral; D003101:Collodion