Zintrons in rat alpha-lactalbumin gene.
The eukaryotic genome is characterised by the occurrence of a large amount of repetitive DNA which exists within and around coding sequences. Random repeats of (TG)n sequences have been observed in several introns. In this paper we show that (TG)14 and (TG)24 sequences present in the third intron of rat alpha-lactalbumin gene adopt left-handed Z-helices under varying superhelical densities. The overall sequence of the parent plasmid further influenced the level of supercoiling at which the B to Z transition could be induced in (TG)n sequences. Such Z-potential intervening sequences (zintrons) could act as buffers to maintain desired levels of supercoiling near the transcribed sequences.
Meera
G
G
Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
Ramesh
N
N
Brahmachari
S K
SK
D000818:Animals; D001482:Base Composition; D004278:DNA, Superhelical; D015180:Electrophoresis, Gel, Two-Dimensional; D007438:Introns; D007768:Lactalbumin / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010957:Plasmids; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid
D004278:DNA, Superhelical; D007768:Lactalbumin