title: Release of taurine and GABA from cerebellar slices from developing and adult mice.
abstract: The properties of the release of exogenous radioactive taurine and GABA from cerebellar slices from developing and adult mice were investigated using a superfusion system. Potassium stimulation (50 mM K+) caused, approximately, a 1.4-fold enhancement in the release of preloaded taurine from slices from adult mice, while the response to potassium in 7-day-old mice was about 6-fold. The potassium stimulation caused, approximately, a 3-fold increase in the release of preloaded GABA from cerebellar slices from 7-day-old mice, whereas the enhancement was about 10-fold in the adult. The actual molar amount of taurine released from the immature cerebellum was strikingly large, about 16 times larger than the amount of GABA released upon the same stimulus. Spontaneous taurine efflux was potentiated by taurine and GABA, the responses being more pronounced in the 7-day-old cerebellum, suggesting that the immature cerebellum is more prone to stimulation by homo- and heteroexchange than the mature cerebellum. Potassium-stimulated taurine release was inhibited by GABAergic substances in the adult but not in the developing cerebellum. Potassium-stimulated GABA release from the adult cerebellum was greatly increased by GABA and also moderately by muscimol and bicuculline, the effect of the latter being antagonized by taurine and hypotaurine. Taurine was thus able to modulate GABA release through bicuculline-sensitive receptors, but this modulation was not evident in cerebellar slices from 7-day-old mice. An exposure of the slices to sodium-free media greatly enhanced taurine and GABA release in both age groups. The stimulated release of GABA generally exhibited a similar calcium dependency in the adult and 7-day-old cerebella but in 7-day-old mice the stimulated release of taurine was not strictly calcium-dependent.
authors.affiliation: Tampere Brain Research Center, Department of Biomedical Sciences, University of Tampere, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Kontro
authors.forename: S S
authors.initials: SS
authors.lastname: Oja
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002531:Cerebellum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D009118:Muscimol / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D013654:Taurine / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D013654:Taurine; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D011188:Potassium; D001640:Bicuculline