Synergism between substrate and non-substrate thiols in peroxidase-oxidase reactions.
Cysteamine and reduced glutathione were shown to act synergistically as peroxidase-oxidase substrates as measured by oxygen consumption and Nitro Blue Tetrazolium reduction. Cysteine methyl ester could be substituted for cysteamine and N-acetylcysteine and penicillamine could be substituted for glutathione. The involvement of reduced oxygen species and the effects of pH and chloride were studied. A possible mechanism of peroxidase-oxidase oxidation of cysteamine and glutathione is proposed. These studies show that peroxidase oxidase reactions can occur with close to physiological concentrations of peroxidase and thiols.
Svensson
B E
BE
Research and Development Laboratories, Södertälje, Sweden.
D002851:Chromatography, High Pressure Liquid; D003543:Cysteamine / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010101:Oxygen Consumption; D010544:Peroxidases / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D013438:Sulfhydryl Compounds; D012965:Sodium Chloride; D003543:Cysteamine; D010088:Oxidoreductases; D010544:Peroxidases; D005978:Glutathione