Induction of altered hepatocellular foci of hamster for a possible short-term assay for carcinogens.
Altered hepatocellular foci of hamster were applied for in vivo short-term (8 weeks) assay for detecting carcinogens using dietary exposure of N-2-fluorenylacetamide (FAA) and oral administration of carbon tetrachloride (CCl4). Although no hepatocellular foci were induced by the treatment with either of two agents (FAA and CCl4) or with their combination, many of the lesions appeared in the hamsters with prior administration of carcinogens, i.e., dimethylnitrosamine, diethylnitrosamine and methylazoxymethanol acetate, all of which have been reported to be carcinogenic in hamsters. A small number of these lesions was also present with the pretreatment of other types of carcinogens, i.e., N-methyl-N-nitrosourea, N-methyl-N'-nitro-N-nitrosoguanidine and azaserine. No such foci were available by N-butyl-N-(4-hydroxybutyl)nitrosamine and 3,3'-dichloro-4,4'-diaminodiphenylmethane, both of which are not known to be clearly carcinogenic in hamsters. The results indicate that hepatocellular foci of hamsters are rapidly inducible under the present protocol and imply that this model could be useful as an assay for screening carcinogens of which hamster is susceptible.
Mori
H
H
Department of Pathology, Gifu University School of Medicine, Japan.
Tanaka
T
T
Niwa
K
K
Yoshimi
N
N
Iwata
H
H
Mori
Y
Y
Hara
A
A
D015073:2-Acetylaminofluorene / Q000633:toxicity; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity*; D006224:Cricetinae; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008647:Mesocricetus; D009929:Organ Size / Q000187:drug effects
D002273:Carcinogens; D015073:2-Acetylaminofluorene; D002251:Carbon Tetrachloride