Introduction of human DNA into mouse eggs by injection of dissected chromosome fragments.
A procedure has been developed for introducing exogenous DNA into mouse eggs by injection of chromosome fragments. Chromosome fragments were dissected from human metaphase spreads and microinjected into the pronuclei of fertilized mouse eggs. Many of the injected eggs subsequently exhibited normal pre- and postimplantation development. Embryos obtained from eggs injected with centromeric fragments retained human centromeric DNA as demonstrated by in situ hybridization analysis. From eggs injected with noncentromeric fragments, a mouse was obtained whose tail tissue exhibited the presence of human DNA. This procedure should facilitate incorporation of very large (more than 10 megabases) DNA fragments into cells and embryos without the need for cloned sequences.
Richa
J
J
Biology Department, University of Pennsylvania, Philadelphia 19104.
Lo
C W
CW
D000818:Animals; D001755:Blastocyst; D002460:Cell Line; D002503:Centromere; D002877:Chromosomes, Human; D004247:DNA / Q000235:genetics*; D006801:Humans; D008677:Metaphase; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D009693:Nucleic Acid Hybridization; D010063:Ovum; D014162:Transfection
D004247:DNA