title: Fertilization and development of mouse eggs injected under the zona pellucida with single spermatozoa treated to induce the acrosome reaction.
abstract: Mouse spermatozoa were capacitated and acrosome reacted by incubation for 30 min to 6 h in modified Tyrode's medium (T6) and in cGMP. The fertilization rates of eggs microinjected with a spermatozoon from samples incubated for 30 min, 2 h, and 6 h in T6 and in cGMP were 36%, 34%, 29%, and 43%, respectively. These rates were not correlated to the percentage of acrosome-reacted spermatozoa identified after these treatments. The lack of association could be explained by the selection of an increased proportion of acrosome-reacted spermatozoa actually used for microinjection, particularly in samples incubated for 30 min and 2 h in T6. Both acrosome-intact and acrosome-reacted spermatozoa were identified under the zona in the eggs which failed to fertilize. Fertilized eggs developed at very high rates to the blastocyst stage in vitro.
authors.affiliation: Centre for Early Human Development, Monash University, Monash Medical Centre, Clayton, Victoria, Australia.
authors.forename: O
authors.initials: O
authors.lastname: Lacham
authors.forename: A
authors.initials: A
authors.lastname: Trounson
authors.forename: C
authors.initials: C
authors.lastname: Holden
authors.forename: J
authors.initials: J
authors.lastname: Mann
authors.forename: H
authors.initials: H
authors.lastname: Sathananthan
mesh_terms: D000177:Acrosome / Q000502:physiology*; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D008297:Male; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000502:physiology; D010063:Ovum / Q000502:physiology*; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*