Application of optimization procedures for the separation of anabolic compounds by high-performance liquid chromatography.
A simple systematic approach is presented for optimizing high-performance liquid chromatographic separations of anabolics with multi-component isocratic mobile phases. A computer program was obtained and adjusted for use with an IBM-compatible XT personal computer. The program requires experimental retention data with three quaternary solvent mixtures to calculate the optimum solvent composition using a geometric model of a prism. For each possible composition of the mobile phase the set of retention data can be calculated. Applications are shown for mixtures of anabolic compounds using a mobile phase composed of methanol, tetrahydrofuran and acetonitrile. The predicted retention data agreed very well with the experimental data.
Jansen
E H
EH
Laboratory for Clinical Chemistry, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Both-Miedema
R
R
van den Berg
R H
RH
D045930:Anabolic Agents / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D013267:Stilbenes / Q000032:analysis; D015029:Zeranol / Q000031:analogs & derivatives / Q000032:analysis
D045930:Anabolic Agents; D013267:Stilbenes; D015029:Zeranol