[The presence of nucleotide sequences of the hepatitis B virus in peripheral lymphocytes of patients with acute hepatitis B].
Hepatitis B virus (HBV) DNA sequences were assessed in 26 patients with acute type B hepatitis, using dot-blot hybridization technique from peripheral blood mononuclear cells (PBMC), during different phases of the illness. At clinical presentation, 15% of patients showed HBV-DNA sequences in PBMC, while serum HBV-DNA was detected in 58% of patients. During clinical improvement 50% of patients had HBV-DNA in PBMC but only 11.5% were positive for serum HBV-DNA. Twenty-three (88.5%) patients recovered and cleared HBV-DNA from serum and from PBMC; three (11.5%) patients with acute hepatitis progressing to chronicity showed persistently HBV-DNA sequences in serum and in PBMC. In conclusion, our study shows that HBV-DNA sequences may be found in PBMC, transiently in patients with acute hepatitis followed by recovery, persistently in patients with acute hepatitis progressing to chronicity.
Lai
M E
ME
Università degli Studi di Cagliari, Istituto di Medicina Interna.
Farci
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P
Figus
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Mulargia
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AP
Orgiana
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Cuboni
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C
Angioni
G
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Balestrieri
A
A
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D015415:Biomarkers / Q000097:blood; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005260:Female; D006509:Hepatitis B / Q000097:blood / Q000235:genetics* / Q000382:microbiology; D006510:Hepatitis B Antibodies / Q000032:analysis; D006511:Hepatitis B Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics*; D006521:Hepatitis, Chronic / Q000097:blood / Q000235:genetics / Q000382:microbiology; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization
D015415:Biomarkers; D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens
Presenza di sequenze nucleotidiche del virus dell'epatite B nei linfociti periferici di pazienti con epatite acuta di tipo B.