A sensitive and specific ELISA using monoclonal capture antibodies for the detection of HLA antigens in blood stains.
A double antibody ELISA technique is described to detect HLA antigens in extracts of blood stains. The assay involves capture of free HLA determinants using an immobilized monoclonal antibody directed against monomorphic regions of HLA class I and HLA class II antigens. The captured antigens are then detected using alloantisera directed against the polymorphic regions of the captured HLA entities. The technique is able to detect specific HLA-A, B, and DR antigens in extracts prepared from blood smears as well as from dried and freshly thawed lymphocytes. The assay may be of potential use in forensic medicine, particularly in instances where extraction of nucleic acids for fingerprinting is not feasible.
Bishara
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A
Department of Clinical Microbiology, Hadassah University Hospital, Jerusalem, Israel.
Brautbar
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C
D001801:Blood Stains; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005554:Forensic Medicine / Q000379:methods*; D006680:HLA Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D011110:Polymorphism, Genetic
D006680:HLA Antigens; D006684:HLA-DR Antigens