Restoration of high affinity choline uptake in the hippocampal formation following septal cell suspension transplants in rats with fimbria-fornix lesions.
High affinity choline uptake (HACU) was investigated in the hippocampal formation following fetal septal cell suspension transplants into rats with fimbria-fornix lesions. Nine-14 weeks after transplantation, HACU was markedly decreased in hippocampi from animals with fimbria-fornix lesions; this decrease was ameliorated by fetal septal cells transplanted into the host hippocampus. HACU related to septal transplantation was activated in vitro by K+, and in vivo by the administration of scopolamine and picrotoxin. These findings suggest that fetal septal cell transplantation can restore HACU in the host hippocampus following fimbria-fornix lesions, and that HACU related to the graft has pharmacological properties similar to those of the normal adult HACU system. The activation of HACU by picrotoxin, a gamma-aminobutyric acid (GABA) antagonist, suggests that transplanted cholinergic neurons receive either direct or indirect functional input from GABAergic afferents from the transplant and/or host hippocampus. Lesions of the fimbria-fornix also resulted in an increased binding to muscarinic receptors in the dorsal hippocampus. This increase in binding was not significantly ameliorated by intrahippocampal grafts of cholinergic neurons.
Kaseda
Y
Y
Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46223.
Simon
J R
JR
Low
W C
WC
D000818:Animals; D001927:Brain Diseases / Q000378:metabolism*; D002794:Choline / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010852:Picrotoxin / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012601:Scopolamine / Q000494:pharmacology; D012686:Septal Nuclei / Q000166:cytology / Q000637:transplantation; D013574:Synaptosomes / Q000378:metabolism; D014018:Tissue Distribution
D002794:Choline; D009419:Nerve Tissue Proteins; D010852:Picrotoxin; D011188:Potassium; D011813:Quinuclidinyl Benzilate; D012601:Scopolamine