title: M.FokI methylates adenine in both strands of its asymmetric recognition sequence.
abstract: M.FokI, a type-IIS modification enzyme from Flavobacterium okeanokoites, was purified, and its activity was characterized in vitro. The enzyme was found to be a DNA-adenine methyltransferase and to methylate both strands of the asymmetric FokI recognition sequence: (formula; see text) M.FokI does not methylate single-stranded DNA, nor does it methylate double-stranded DNA at sequences other than FokI sites.
authors.affiliation: New England Biolabs, Inc., Beverly, MA 01915.
authors.forename: D
authors.initials: D
authors.lastname: Landry
authors.forename: M C
authors.initials: MC
authors.lastname: Looney
authors.forename: G R
authors.initials: GR
authors.lastname: Feehery
authors.forename: B E
authors.initials: BE
authors.lastname: Slatko
authors.forename: W E
authors.initials: WE
authors.lastname: Jack
authors.forename: I
authors.initials: I
authors.lastname: Schildkraut
authors.forename: G G
authors.initials: GG
authors.lastname: Wilson
mesh_terms: D000225:Adenine / Q000378:metabolism*; D000595:Amino Acid Sequence; D002855:Chromatography, Thin Layer; D004247:DNA / Q000378:metabolism; D005417:Flavobacterium / Q000201:enzymology* / Q000235:genetics; D008745:Methylation; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; C058668:DNA modification methylase FokI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D000225:Adenine