Molecular surface characterization of oral streptococci by Fourier transform infrared spectroscopy.
In order to characterize the molecular composition of oral streptococci, infrared transmission spectroscopy on freeze-dried cells dissolved in KBr was used. All infrared spectra show similar absorption bands for the strains studied with the most important absorption bands located at 2930 cm-1 (CH), 1653 cm-1 (AmI), 1541 cm-1 (AmII) and two bands at 1236 cm-1 and 1082 cm-1, which were assigned to phosphate and sugar groups. However, calculation of absorption band ratios normalized with respect to the integrated intensity of the CH stretching region around 2930 cm-1, show significant differences between the strains. Both Streptococcus mitis strains possess high AmI/CH and AmII/CH absorption band ratios compared to the other strains. Streptococcus salivarius HBC12, a mutant strain devoid of all proteinaceous surface appendages, shows significantly lower AmI/CH and AmII/CH band ratios with respect to its parent strain S. salivarius HB. Two positive relationships could be established both between the AmII/CH absorption band ratio and the N/C elemental surface concentration ratio of the strains previously, determined from X-ray photoelectron spectroscopy (XPS) and also between AmI/CH and the fraction of carbon atoms at the surface involved in amide bonds, determined by XPS as well. From this comparison, it is concluded that transmission infrared spectroscopy can be employed as a technique to study the molecular surface composition of freeze-dried microorganisms.
van der Mei
H C
HC
Laboratory for Materia Technica, University of Groningen, The Netherlands.
Noordmans
J
J
Busscher
H J
HJ
D000577:Amides / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D005583:Fourier Analysis; D006801:Humans; D009055:Mouth / Q000382:microbiology; D010710:Phosphates / Q000032:analysis; D013055:Spectrophotometry, Infrared / Q000379:methods; D013291:Streptococcus / Q000032:analysis*; D013295:Streptococcus mutans / Q000032:analysis; D013298:Streptococcus sanguis / Q000032:analysis
D000577:Amides; D002241:Carbohydrates; D010710:Phosphates