Clonal allele loss in gastrointestinal cancers.
Using a panel of DNA probes for hypervariable DNA regions we screened 52 gastrointestinal carcinomas for clonal allele losses on chromosomes 1, 5, 7, 12, 16 and 17. A total of 24/35 informative cases of colorectal cancers showed loss of constitutional heterozygosity at a locus on chromosome 17p, while 9/31 cases informative for a locus on 5q showed allele loss. Loss of sequences at 5q was linked to allele loss at 17p with a single exception. In gastric cancers loss of heterozygosity most frequently occurred at 1q (5/10 tumours) and at 12q (6/11 tumours). Gastrointestinal tumours show consistent chromosomal losses and the loci involved are different in gastric and colorectal cancers.
Fey
M F
MF
Department of Haematology, John Radcliffe Hospital, Headington, Oxford, UK.
Hesketh
C
C
Wainscoat
J S
JS
Gendler
S
S
Thein
S L
SL
D000230:Adenocarcinoma / Q000235:genetics*; D000483:Alleles; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D015179:Colorectal Neoplasms / Q000235:genetics*; D015342:DNA Probes; D006579:Heterozygote; D006801:Humans; D013274:Stomach Neoplasms / Q000235:genetics*
D015342:DNA Probes