Influence of shear stress on tumor-cell adhesion to endothelial-cell extracellular matrix and its modulation by fibronectin.
The interaction of malignant cells with blood-vessel endothelial cells and their underlying basement membrane is an important step in the development of secondary metastases. We investigated the interactions of highly metastatic human tumor cells, the A-549 adenocarcinoma of the lung, with cultured endothelial cells (EC) and their extracellular matrix (ECM). We studied the adhesion patterns of the A-549 tumor cells to EC and ECM under static and flow conditions. Our results provide evidence that tumor-cell adhesion depends not only on the characteristics of the tumor cells themselves, but also on the properties of the EC and ECM. Our results also indicate that tumor-cell adhesion to ECM is shear-rate-dependent, and that it is partially modulated by fibronectin. Moreover, our results suggest that the arg-gly-asp (RGD) common adhesion receptor site is also involved in the adhesion of the A-549 cells to EC and ECM.
Bastida
E
E
Servicio de Hemoterapia y Hemostasia, Hospital Clinic i Provincial de Barcelona, Spain.
Almirall
L
L
Bertomeu
M C
MC
Ordinas
A
A
D000230:Adenocarcinoma / Q000473:pathology*; D000906:Antibodies / Q000008:administration & dosage; D001485:Basement Membrane / Q000166:cytology / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects / Q000648:ultrastructure*; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D009362:Neoplasm Metastasis; D010455:Peptides / Q000494:pharmacology; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D013314:Stress, Mechanical; D014407:Tumor Cells, Cultured
D000906:Antibodies; D005353:Fibronectins; D010455:Peptides