Characterization of a YAC-1 mouse cell receptor for group B coxsackieviruses.
A receptor on YAC-1 cells, a mouse T-lymphoma cell line, bound all six serotypes of the group B coxsackieviruses (CVB). In addition, the cells produced infectious virus. Each of the CVB competed for the same receptor on YAC-1 cells. CVB3 bound relatively slowly to YAC-1 cells (k = 4 x 10(-11) min-1 cell-1), and there were only 500 attachment sites per cell. A rabbit antiserum prepared against the HeLa cell receptor protein Rp-a specifically inhibited the binding of CVB1 and CVB3. A virus-receptor complex with CVB3 could be isolated from detergent (0.5% sodium deoxycholate, 1% Triton X-100)-solubilized YAC-1 plasma membranes. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the iodinated virus-receptor complex revealed a band with the same mobility as Rp-a. The results suggested that the YAC-1 receptor for CVB resembles that of the HeLa cell receptor.
Hsu
K H
KH
Department of Microbiology and Immunology, Hahnemann University School of Medicine, Philadelphia, Pennsylvania 19102-1192.
Crowell
R L
RL
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D004770:Enterovirus / Q000502:physiology*; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007700:Kinetics; D008223:Lymphoma; D051379:Mice; D011991:Receptors, Virus / Q000502:physiology*
D011991:Receptors, Virus