[Study on the kinetics of isolation of trypsin immobilized on dialdehyde cellulose during hydrolytic destruction].
The kinetics of isolation of trypsin immobilized on a dialdehyde cellulose carrier in buffer solutions with pH 7.0 and 8.0 was studied. The number of the aldehyde groups amounted to 15-60 per cent of the initial one. The kinetics of hydrolytic destruction of the collected samples with the immobilized trypsin and the carrier in buffer solutions with pH 7.0 and 8.0 was also studied. The constants of the rates of trypsin isolation from the materials and the constants of the rates of liberation into the buffer solutions of the compounds containing CO-groups were determined. The groups were used for estimating hydrolytic destruction of the materials and the time of complete destruction of the materials in the solutions.
Ryl'tsev
V V
VV
Virnik
R B
RB
D002482:Cellulose / Q000031:analogs & derivatives*; D004800:Enzymes, Immobilized / Q000302:isolation & purification* / Q000493:pharmacokinetics; D006868:Hydrolysis; D014677:Pharmaceutical Vehicles; D014357:Trypsin / Q000302:isolation & purification* / Q000493:pharmacokinetics
D004800:Enzymes, Immobilized; D014677:Pharmaceutical Vehicles; D002482:Cellulose; C058684:2,3-dialdehydocellulose; D014357:Trypsin
Issledovanie kinetiki vydeleniia tripsina, immobilizovannogo na dial'degidtselliuloze, pri gidroliticheskoĭ destruktsii.