title: [Interaction of immunoglobulins with charged biopolymers: significance in the regulation of humoral immunity].
abstract: When subjected to ion exchange chromatography on QAE-Sephadex A-50 or gel filtration of Sephadex G-200 under conditions that cause the dissociation of immune complexes at pH 4.05, immunoglobulins both from serum and its immunoglobulin fraction increase their interaction with charged antigens as native DNA and cardiolipin. Ion exchange chromatography also leads to the deaggregation of complexes. It was demonstrated that immunoglobulins bind DNA molecule through its F(ab)2 fragments. Based on data obtained, the suggestion was made that interaction between immunoglobulins and charged serum biopolymers is an important factor in humoral immunity regulation. Namely, high specificity of immunological reactions may be supported by elimination of non-specific binding provided electrostatic interactions from all the potential spectrum of antigen-antibody reactions.
authors.forename: A M
authors.initials: AM
authors.lastname: Poverennyĭ
authors.forename: G M
authors.initials: GM
authors.lastname: Rott
authors.forename: N I
authors.initials: NI
authors.lastname: Sulaeva
authors.forename: I V
authors.initials: IV
authors.lastname: Lekakh
authors.forename: V A
authors.initials: VA
authors.lastname: Saenko
mesh_terms: D000917:Antibody Formation; D001704:Biopolymers / Q000378:metabolism*; D002308:Cardiolipins / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000378:metabolism*; D004560:Electricity; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D007525:Isoelectric Focusing; D046911:Macromolecular Substances / Q000378:metabolism*; D011863:Radioimmunoassay
chemical_list: D001704:Biopolymers; D002308:Cardiolipins; D007136:Immunoglobulins; D046911:Macromolecular Substances; D004247:DNA
vernacular_title: Vzaimodeĭstvie immunoglobulinov s zariazhennymi biopolimerami: znachenie v reguliatsii gumoral'nogo immuniteta.