[Various features of peroxidase in human saliva].
Optimal conditions are determined for isolation and purification of peroxidase (EC 1.11.1.7) from the mixed human saliva by gel-filtration and ion chromatography. Such properties as pH and temperature optima, molecular weight, Km, amino acid composition, the effect of activators and inhibitors (purified saliva peroxidases and commercial preparation of horse-radish peroxidase "Reanal", Hungary) are studied with the use of different substrates. The data obtained are necessary to optimize production processes of isolation, storage and methods of the experimental and clinical application of peroxidases of different origin.
Tkachenko
E K
EK
Barabash
R D
RD
Vovchuk
S V
SV
D000596:Amino Acids / Q000032:analysis; D002384:Catalysis; D002850:Chromatography, Gel; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010544:Peroxidases / Q000378:metabolism*; D012463:Saliva / Q000201:enzymology*; D013696:Temperature
D000596:Amino Acids; D006735:Horseradish Peroxidase; D010544:Peroxidases
Nekotorye svoìstva peroksidazy sliuny cheloveka.