An enzyme immunoassay for estimating small numbers of cells.
In this report we describe an enzyme immunoassay for determination of cell number in small samples. The assay utilizes a commercially available monoclonal antibody that recognizes double- and single-stranded DNA and works with several different methods of cell fixation. DNA extraction is not required. Confluent, growth-arrested fibroblasts serve as standards. While most readily applicable to anchorage-dependent cells, addition of a centrifugation step allows the method to be applied also to anchorage-independent cells.
Faraji-Shadan
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F
Letterman Army Institute of Research, Presidio of San Francisco, California 94129-6800.
Bowman
P D
PD
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D002452:Cell Count / Q000379:methods; D002455:Cell Division; D002460:Cell Line; D002477:Cells / Q000166:cytology*; D004247:DNA / Q000032:analysis* / Q000276:immunology; D004273:DNA, Neoplasm / Q000032:analysis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D004273:DNA, Neoplasm; D004247:DNA