Induction of neurite outgrowth from chick embryonic ganglia explants by activators of protein kinase C.
Neurite outgrowth from chick embryonic sensory ganglia explants was induced by activators of protein kinase C and other compounds known to stimulate the hydrolysis of inositol phospholipids. The addition of diacylglycerols, phospholipase C and muscarine chloride to a defined growth medium promoted the outgrowth of dense neurites from ganglia explants which were morphologically distinct from those induced by the phorbol ester TPA. Moreover, these neurite-promoting agents did not enhance non-neuronal cell proliferation and were ineffective in the absence of insulin and/or progesterone.
Hsu
L
L
Department of Biology, Seton Hall University, South Orange, NJ 07079.
Jeng
A Y
AY
Chen
K Y
KY
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure*; D002478:Cells, Cultured; D002642:Chick Embryo; D004075:Diglycerides / Q000494:pharmacology*; D004789:Enzyme Activation; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects; D005989:Glycerides / Q000494:pharmacology*; D009116:Muscarine / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004075:Diglycerides; D005989:Glycerides; D009116:Muscarine; C038237:1-oleoyl-2-acetylglycerol; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate