[Conditions for continuous registration of enzyme activity].
Conditions of continuous registration of enzyme activity are considered on the example of alcohol dehydrogenase and sorbitol dehydrogenase from cytoplasm of the bovine liver cells. A device permitting to register the initial steps of enzyme interaction with the effector (substrate, coenzyme or inhibitor) is described. The importance of the reaction product coupling for analysis of enzyme activity is demonstrated.
Sudovtsov
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VE
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004798:Enzymes / Q000378:metabolism*; D066298:In Vitro Techniques; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D013053:Spectrophotometry / Q000295:instrumentation; D013997:Time Factors
D004798:Enzymes; D000426:Alcohol Dehydrogenase; D007064:L-Iditol 2-Dehydrogenase
Usloviia nepreryvnoĭ registratsii fermentativnoĭ aktivnosti.