Different patterns of G/T and A/C mismatch repair in simian cells correspond to the specificity of a mismatch binding protein isolated from simian and HeLa cells.
The two transition mispairs G/T and A/C are corrected with different efficiencies and specificities in CV-1 African green monkey kidney cells. G/T mispairs are corrected with 99% efficiency and almost always in favor of guanine, while A/C mispairs exhibit a 31% uncorrected sector and are otherwise randomly corrected. The higher correction efficiency and bias for the G/T mispair can be correlated with the substrate specificity of a protein, found in HeLa and CV-1 cell extracts, that binds selectively to oligonucleotide duplexes containing G/T mispairs. No binding can be detected to duplexes containing other mispairs, or to homoduplexes.
Brown
T C
TC
Corman
N
N
Rudkin
B B
BB
Jiricny
J
J
D000225:Adenine / Q000032:analysis; D000818:Animals; D001482:Base Composition; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003596:Cytosine / Q000032:analysis; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D006147:Guanine / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D013941:Thymine / Q000032:analysis
D004268:DNA-Binding Proteins; D006147:Guanine; D003596:Cytosine; D000225:Adenine; D013941:Thymine