Frequency of occurrence of abnormal cell types in organ-cultured fetal crystalline lenses and its dependence upon the culture medium used.
Statistically significant differences were found to exist in the frequencies of abnormal cell types in cultured lenses depending on the composition of the nutrient media (Eagle's and Dulbecco's modified essential medium with fetal calf serum FCS) they were cultured on. Abnormal cell growth (epithelioid, fibroblast-like, infiltrating or bladder cells) was more frequent in lenses grown in the nutrient media containing either the Eagle medium or in the nutrient media containing 20% FCS. Bladder cells were more frequent in the Eagle medium, whereas fibroblast-like cells occurred more often in the Eagle medium with 20% FCS.
Veroman
S
S
Institute of General and Molecular Pathology, Tartu State University, Estonia, USSR.
Viikmaa
M
M
D000818:Animals; D002417:Cattle; D003470:Culture Media; D004847:Epithelial Cells; D005312:Fetal Blood; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology; D007908:Lens, Crystalline / Q000166:cytology*; D009924:Organ Culture Techniques; D013552:Swine; D013997:Time Factors; D001743:Urinary Bladder / Q000166:cytology
D003470:Culture Media