[A study of the conformation of native and immobilized human somatotropin using circular dichroism].
The conformation of human growth hormone (hGH) as monomer and aggregate as well as of those immobilized in the soluble polysaccharide matrix was investigated. The immobilization resulted in ten per cent decrease of the amount of alpha-helix and in the conformational mobility change of aromatic amino acid side groups. It did not, however, influence the biological activity of the hormone, thus suggesting that the above conformational variations did not affect functionally important portions of the molecule.
Vareĭkis
E I
EI
Lobachev
V M
VM
Iuodka
B A
BA
D002942:Circular Dichroism; D013006:Growth Hormone; D006801:Humans; D011487:Protein Conformation
D013006:Growth Hormone
Issledovanie konformatsii nativnogo i immobilizovannogo somatotropina cheloveka s pomoshch'iu metoda krugovogo dikhroizma.