title: Effect of route of administration in the micronucleus test with potassium bromate.
abstract: The effect of intraperitoneal injection (i.p.) versus oral gavage administration (p.o.) of potassium bromate was examined using the micronucleus test in 2 strains of male mice (MS/Ae and CD-1). First, a small acute toxicity test and a pilot micronucleus experiment were performed to determine the appropriate dose range and sampling time for the full-scale micronucleus test. The full-scale test was carried out using doses of 18.8, 37.5, 75, and 150 mg/kg in the i.p. test and of 37.5, 75, 150, and 300 mg/kg in the p.o. test. The sampling time was 24 h for both mouse strains. Potassium bromate induced micronucleated polychromatic erythrocytes (MNPCEs) dose-dependently by both routes of administration in both mouse strains. No distinct difference in route of administration was observed in the test with MS/Ae mice. In CD-1 mice more MNPCEs were induced by the i.p. route than by the p.o. route.
authors.affiliation: Biosafety Research Center, Foods, Drugs, and Pesticides (An-Pyo Center), Shizuoka-ken, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
authors.forename: M
authors.initials: M
authors.lastname: Kitazawa
authors.forename: K
authors.initials: K
authors.lastname: Oba
authors.forename: Y
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001959:Bromates / Q000008:administration & dosage* / Q000633:toxicity; D001966:Bromine / Q000008:administration & dosage*; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage*; D010865:Pilot Projects
chemical_list: D001959:Bromates; D009153:Mutagens; C019536:potassium bromate; D001966:Bromine