title: Cochlear degeneration in minipigs after repeated hyperbaric exposures.
abstract: Auditory function and cochlear pathology were investigated in 4 minipigs subjected to compression to 4 ATA, held for 1 h, and decompressed using a standard schedule (Blackpool Tables) on 21 occasions. Three minipigs were used as controls. Brainstem auditory evoked response testing was carried out after the last hyperbaric exposure and showed no response bilaterally in 3 and unilaterally in 1 of the test animals. Light microscopy demonstrated a loss of hair cells throughout the cochlea in all the compressed animals; in one the tectorial membrane was detached and adherent to Reissner's membrane. Vestibular changes were confined to the saccule. Hemorrhage was not a prominent feature. On scanning electron microscopy the pathologic changes included missing hair cells and fused and giant stereocilia. Possible causes of the pathology include barotrauma or direct effects of repeated compression and decompression on the inner ear, or both. The compression-decompression profile used was one that is thought to be safe for compressed air workers who are repeatedly exposed to hyperbaric conditions.
authors.affiliation: Wellcome Laboratory for Comparative Neurology, Department of Clinical Veterinary Medicine, Cambridge, England.
authors.forename: M K
authors.initials: MK
authors.lastname: Wilkes
authors.forename: A C
authors.initials: AC
authors.lastname: Palmer
authors.forename: P C
authors.initials: PC
authors.lastname: Pearce
authors.forename: N P
authors.initials: NP
authors.lastname: Luff
authors.forename: M J
authors.initials: MJ
authors.lastname: Halsey
authors.forename: I M
authors.initials: IM
authors.lastname: Calder
mesh_terms: D000818:Animals; D001469:Barotrauma / Q000473:pathology* / Q000503:physiopathology; D001933:Brain Stem / Q000503:physiopathology; D003051:Cochlea / Q000293:injuries* / Q000473:pathology; D005072:Evoked Potentials, Auditory; D006198:Hair Cells, Auditory / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D013552:Swine; D013556:Swine, Miniature