title: Molecular cloning and characterization of the primary structure of the alkane hydroxylating cytochrome P-450 from the yeast Candida maltosa.
abstract: A cDNA library was established starting from poly(A) RNA of n-alkane-grown Candida maltosa cells and cDNA clones were isolated containing the entire coding sequence for the alkane hydroxylating cytochrome P-450. The deduced protein consists of 521 amino acids, contains two putative transmembrane segments in the N-terminal region and has a characteristic heme-binding sequence in the C-terminal part. Sequence alignments with members of 11 reported cytochrome P-450 families revealed a strong homology to an alkane-inducible cytochrome P-450 from Candida tropicalis.
authors.affiliation: Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin-Buch.
authors.forename: W H
authors.initials: WH
authors.lastname: Schunck
authors.forename: E
authors.initials: E
authors.lastname: Kärgel
authors.forename: B
authors.initials: B
authors.lastname: Gross
authors.forename: B
authors.initials: B
authors.lastname: Wiedmann
authors.forename: S
authors.initials: S
authors.lastname: Mauersberger
authors.forename: K
authors.initials: K
authors.lastname: Köpke
authors.forename: U
authors.initials: U
authors.lastname: Kiessling
authors.forename: M
authors.initials: M
authors.lastname: Strauss
authors.forename: M
authors.initials: M
authors.lastname: Gaestel
authors.forename: H G
authors.initials: HG
authors.lastname: Müller
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D005810:Multigene Family
chemical_list: D008565:Membrane Proteins; D004247:DNA; D003577:Cytochrome P-450 Enzyme System