Labeling of DNA probes with a photoactivatable hapten.
A photoactivatable reagent for introducing haptens onto DNA probes has been prepared using a commercially available bifunctional linker arm reagent and amino-derivatized 2,4-dinitrophenyl (DNP). The resulting compound (photo-DNP) couples efficiently to DNA using an ordinary sunlamp. Under optimum conditions, about 7-23 DNP molecules per 1000 bases are incorporated into the DNA. Hybridization experiments demonstrate that as little as 1.5 x 10(5) copies of target DNA can be detected by filter hybridization with a photo-DNP-labeled probe and immunochemical detection.
Keller
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Biotech Research Laboratories, Inc., Rockville, Maryland 20850.
Huang
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DP
Manak
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D000614:Aminocaproates / Q000138:chemical synthesis*; D015119:Aminocaproic Acid / Q000138:chemical synthesis*; D001386:Azides / Q000138:chemical synthesis*; D015342:DNA Probes; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics; D006241:Haptens; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D015151:Immunoblotting / Q000379:methods; D010777:Photochemistry; D010957:Plasmids; D011089:Polydeoxyribonucleotides
D000614:Aminocaproates; D001386:Azides; D015342:DNA Probes; D006241:Haptens; D011089:Polydeoxyribonucleotides; C059874:6-(2,4-dinitrophenylamino)-1-aminohexyl-6-(4'-azido-2'-nitrophenylamino)hexanoate; D015119:Aminocaproic Acid