Quantitative analysis of the hydrogen peroxide formed in aqueous cigarette tar extracts.
We have established, for the first time, a reliable method to quantitate hydrogen peroxide (H2O2) generated in aqueous extracts of cigarette smoke tar. The aqueous tar extract was passed through a short reverse-phase column and its H2O2 concentration determined by differential pulse polarography using an automatic reference subtraction system. The H2O2 concentration increased with aging, pH and temperature; the presence of superoxide dismutase lead to lower H2O2 concentrations. This method was applied to many kinds of research and commercial cigarettes. With a few exceptions, the amount of H2O2 formed after a fixed time from each cigarette smoke was proportional to its tar yield.
Nakayama
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Biodynamics Institute, Louisiana State University, Baton Rouge 70803.
Church
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Pryor
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D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010947:Plants, Toxic; D011048:Polarography; D012906:Smoke / Q000032:analysis*; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000032:analysis*
D007202:Indicators and Reagents; D012906:Smoke; D006861:Hydrogen Peroxide