[Activity of 5-alpha- and 5-beta-steroid reductases in subcellular liver structures from rats bearing Walker carcinosarcoma].
Change in the activity of cytoplasmic 5 beta- and microsomal 5 alpha-steroid reductases was studied in tumor bearing rats. As shown by thin-layer chromatography conversion of 3H-corticosterone into 5-alpha and 5 beta-dihydro-compounds was decreased 3-fold in rats bearing Walker tumor. Activity of both these enzymes exceeded the initial level 2-3-fold in presence of exogenous NADPH. At the same time, activation of glucose 6-phosphate dehydrogenase was accompanied by simultaneous alteration in content of transcortin-bound corticosterone in rat blood plasma. 5 alpha-reductase was detected in membranes of liver tissue endoplasmic reticulum and in the fraction of free polyribosomes.
Ulinskaĭte
A P
AP
Krauialis
K I
KI
Diashrius
A P
AP
D000818:Animals; D002279:Carcinoma 256, Walker / Q000201:enzymology*; D003345:Corticosterone / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009249:NADP; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology*
D009249:NADP; D010088:Oxidoreductases; D003345:Corticosterone
Aktivnost' 5-alpha- i 5-beta-steroidreduktazy v subkletochnykh strukturakh pecheni krys pri roste kartsinosarkomy uokera.