title: High-performance ion-exchange chromatography of oligoribonucleotides using linear and hyperbolic salt gradients.
abstract: A method for separation and chain length determination of oligo- and polynucleotides by high-performance anion-exchange chromatography was developed, which allows resolution of individual fragments according to their chain length n, up to n approximately 10 by linear gradient of sodium chloride and up to n approximately 30 by an hyperbolic gradient of this salt. The hyperbolic relationship between n and the salt concentration at which elution of the fragment occurs allows determination of the degree of polymerization of oligo- and polynucleotides with unknown n. The method proposed can be used for estimation of the effective charge of nucleic acids with complex structure.
authors.affiliation: A. N. Bakh Institute of Biochemistry, U.S.S.R. Academy of Sciences, Moscow.
authors.lastname: Tsygankov AYu
authors.lastname: Motorin YuA
authors.forename: A D
authors.initials: AD
authors.lastname: Wolfson
authors.forename: D B
authors.initials: DB
authors.lastname: Kirpotin
authors.forename: A F
authors.initials: AF
authors.lastname: Orlovsky
mesh_terms: D002851:Chromatography, High Pressure Liquid; D009843:Oligoribonucleotides / Q000032:analysis*; D011061:Poly A / Q000032:analysis; D011072:Poly U / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis
chemical_list: D009843:Oligoribonucleotides; D011061:Poly A; D011072:Poly U; D012343:RNA, Transfer