An enhancer in the c-mos locus binds a nuclear factor 1-like protein.
We recently identified a DNA sequence element located in the rat c-mos protooncogene which fulfills operational criteria for enhancers, increasing transcription from a heterologous promoter in fibroblasts over large distances and in an orientation-independent manner. Here we report that three mouse nuclear proteins bind to the enhancer. Several lines of evidence indicate that one is a Nuclear Factor 1 like activity. Oligonucleotide-directed mutagenesis of the NF-1 binding site destroys binding of the protein, but leaves enhancer activity unaffected. Tumor growth factor-beta, which was shown to exert a stimulatory effect on the alpha 2(I) collagen promoter via its NF-1 binding site, has no effect on the activity of the enhancer. Thus, the NF-1 binding site of the c-mos enhancer is not essential for its activity in fibroblasts.
van der Hoorn
F A
FA
Department of Pathology, University of Colorado Health Sciences Center, Denver 80262.
Nordeen
S K
SK
D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D011519:Proto-Oncogenes; D051381:Rats; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human