Immunoelectron microscopic visualization of the transcytosis of low density lipoproteins in perfused rat arteries.
A postembedding labeling technique was employed to visualize human native low density lipoproteins (LDL) during transcytosis in rat arterial endothelium. For this purpose human LDL was perfused through rat vasculature before fixation and processing for immunoelectron microscopy. The LDL particles were located on sections by anti-human apolipoprotein B-100 (LDL) antibodies and secondary antibodies or protein-A conjugated to 10-nm colloidal gold. LDL molecules were seen in plasmalemmal vesicles as well as in the subendothelial space. No colloidal gold was found in the intercellular junctions. Perfusion with reductively methylated LDL, which cannot bind to the LDL receptor, gave a similar labeling pattern, indicating that transcytosis of LDL via plasmalemmal vesicles is most likely receptor independent. Furthermore, the passage of LDL through intact vascular endothelium is a vesicular transport rather than an intercellular diffusion process.
Snelting-Havinga
I
I
Department of Dermatology, Leiden University, The Netherlands.
Mommaas
M
M
Van Hinsbergh
V W
VW
Daha
M R
MR
Daems
W T
WT
Vermeer
B J
BJ
D000818:Animals; D001158:Arteries / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D011974:Receptors, LH / Q000378:metabolism / Q000502:physiology
D008077:Lipoproteins, LDL; D011974:Receptors, LH