The automated enzymic assay of D-isocitrate in human urine.
A simple, automated, enzymic assay for the analysis of D-isocitrate in urine is described. The method detects D-isocitrate in the range 0.05-1.00 mmol/L, producing within-batch and between-batch coefficients of variation of 1.8-3.2% and 2.7-3.0% respectively. The assay was also shown to be robust, tolerating extremes of sample pH. Of a wide range of substances tested, only Fe3+ ions interfered, but only at levels considerably in excess of those encountered in urine. D-isocitrate was stable in urine for up to one week at room temperature or at 4 degrees C, with or without preservatives.
Pallanca
J E
JE
Biochemistry Department, St Paul's Hospital, London, UK.
Denney
R C
RC
Rose
G A
GA
D001322:Autoanalysis / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007521:Isocitrate Dehydrogenase; D007523:Isocitrates / Q000652:urine*
D007523:Isocitrates; C034219:isocitric acid; D007521:Isocitrate Dehydrogenase