Capillary zone electrophoresis of histidine-containing compounds.
Capillary zone electrophoresis has been tested for the separation of angiotensins, cationic heptapeptides and model histidine derivatives. Good separation efficiencies are seen for peptides and model compounds with negative to small positive net charges. For net charge greater than +2, addition of putrescine to pH 6 buffer greatly suppresses ion exchange at anionic sites on fused silica. When operating at pH values where histidine groups are neutral, addition of Zn2+ allows separations based on metal, rather than proton, binding. Separation efficiencies and relative migration times are dependent on capillary length when ion-exchange behavior occurs.
Stover
F S
FS
Central Research Laboratories, Monsanto Co., St. Louis, MO 63167.
Haymore
B L
BL
McBeath
R J
RJ
D000809:Angiotensins / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D006639:Histidine / Q000032:analysis*; D006801:Humans; D010455:Peptides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000809:Angiotensins; D010455:Peptides; D006639:Histidine