Biological activities of synthesized 20K and 22K hGH in Nb2 bioassay and IM-9 radioreceptor assay.
We investigated the bioactivities of the recombinant DNA-derived methionyl 20K hGH (20K-Met-hGH) and methionyl hGH (22K-Met-hGH). The growth-promoting activities in Nb2 cells of 20K-Met-hGH and 22K-Met-hGH were 10.7% and 93.5% of pituitary hGH (P-hGH), respectively. In the IM-9 lymphocyte assay, the binding activities of 20K-Met-hGH and 22K-Met-hGH to hGH receptor were 29.0% and 87.1% of P-hGH, respectively. Our data demonstrate that 20K-Met-hGH may have weaker biological potency than P-hGH.
Ohmae
Y
Y
Endocrine Research Laboratory, National Children's Medical Research Center, Tokyo, Japan.
Yano
H
H
Umezawa
S
S
Tanaka
T
T
Hibi
I
I
Miyamoto
C
C
Furuichi
Y
Y
D000818:Animals; D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D013006:Growth Hormone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D006728:Hormones; D019382:Human Growth Hormone; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008223:Lymphoma / Q000473:pathology; D008970:Molecular Weight; D011869:Radioligand Assay; D051381:Rats; D011986:Receptors, Somatotropin / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D006728:Hormones; D011986:Receptors, Somatotropin; D011994:Recombinant Proteins; D019382:Human Growth Hormone; D013006:Growth Hormone