Identification and characterization of the emetic effects of peptide YY.
Emesis was noted following intravenous bolus injections into dogs of a chromatographic subfraction derived from porcine small intestinal tissue extracts. The active agent was isolated from this subfraction using sequential ion-exchange and reverse-phase HPLC and demonstrated to be the recently identified regulatory peptide PYY. The threshold dose for PYY-induced emesis in the dog is less than 120 pmol/kg. Emesis was sometimes seen following large IV bolus doses of neuropeptide Y (NPY), but none was seen following IV injection of pancreatic polypeptide (PP). Dogs prepared with discrete, bilateral lesions of the area postrema were refractory to a suprathreshold emetic dose of PYY. PYY is the most potent, circulating emetic peptide identified to date.
Harding
R K
RK
Defence Research Establishment Ottawa, Ontario, Canada.
McDonald
T J
TJ
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004285:Dogs; D004639:Emetics; D005260:Female; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D007421:Intestine, Small / Q000032:analysis; D008297:Male; D019894:Peptide YY; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D013552:Swine
D004639:Emetics; D005768:Gastrointestinal Hormones; D010455:Peptides; D019894:Peptide YY; D001058:Apomorphine