Light and electron microscope immunolocalization of cytosolic malic enzyme-like activity in the rat's cerebellar cortex.
The immunocytochemical localization of malic enzyme-like activity in the cerebellar cortex of the rat at light and electron microscope levels using the PAP method was investigated. The antiserum against the malic enzyme was obtained from rabbit after having injected the chicken liver malic enzyme. The microscope observations have demonstrated the presence of a positive immunocytochemical reaction against malic enzyme in the cytosol of Bergmann glial cells and in the astrocytes of granular layer and also in several dendrites, but not in all, in the postsynaptic site. A possible role of cytosolic malic isoenzyme (cME) in the several synapses is suggested.
Martínez-Rodríguez
R
R
Cajal Institute of C.S.I.C., Madrid, Spain.
Arenas
G
G
Hidalgo
M M
MM
Carnicero
M B
MB
D000818:Animals; D002525:Cerebellar Cortex / Q000201:enzymology* / Q000648:ultrastructure; D003600:Cytosol / Q000201:enzymology*; D007150:Immunohistochemistry; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
D008291:Malate Dehydrogenase