A high precision dual feedback pump for unsteady perfusion of small organs.
A dynamic pump system is described for perfusion of small organs with whole blood. The pump system was designed with the following aims: Very low flowrates to perfuse single organs in small rodents; high dynamic response for pressure or flow to permit experimenting with a harmonic signal at frequencies up to 20 Hz or by way of sharp step transients in less than 10 msec; high precision to allow detection of fine physiological details, and minimum blood cell trauma or cell activation by use of a piston principle. Representative pressure-flow curves are shown for the rat gracilis muscle after vasodilation. The curves are highly reproducible and serve as a complimentary dataset for microvascular observations in the same organ.
Sutton
D W
DW
AMES-Bioengineering University of California, San Diego, La Jolla 92093.
Mead
E H
EH
Schmid-Schönbein
G W
GW
D000818:Animals; D004867:Equipment Design; D007260:Infusion Pumps; D010477:Perfusion / Q000295:instrumentation*; D011312:Pressure; D011673:Pulsatile Flow; D012377:Rodentia