Loss of focal contacts accompanies the density dependent inhibition of cell growth.
The formation of focal contacts by fibroblast-like 3T3 and 9390 cells and endothelial XTH-2 cells was examined with the use of reflection interference contrast microscopy at different cell densities. It was found that cells density-inhibited in growth loose all focal contacts with the substratum. This observation explains the disappearance of stress fibers in density-inhibited cells, and also offers an explanation for the mechanism of density-dependent inhibition of proliferation of anchorage-dependent cells.
Kajstura
J
J
Cinematic Cell Research Group, Johann Wolfgang Goethe-University.
Bereiter-Hahn
J
J
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003260:Contact Inhibition; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice