Ulcerative colitis: specific antibodies against a colonic epithelial Mr 40,000 protein.
A colon tissue-bound disease specific IgG (CCA-IgG) antibody can be eluted from the colonic tissue of patients with ulcerative colitis (UC). CCA-IgG recognizes an Mr 40,000 protein present in both normal and diseased colon. Using an anti-Mr 40,000 murine monoclonal antibody this protein has been localized exclusively in colonic epithelial cells mainly along the baso-lateral domains of plasma membrane. Non-colonic epithelial tissues from 12 different organs including small intestine did not react with the anti-Mr 40,000 monoclonal antibody. The Mr 40,000 protein is present in the RPMI-4788 colon cancer cells but not in HeLa. The antibody dependent cell-mediated cytolysis by UC sera against RPMI-4788 cells was inhibited by anti-Mr 40,000 polyclonal antibody. These findings suggest that CCA-IgG and the Mr 40,000 colonic epithelial protein are involved in an autoimmune reaction and may be important in the pathogenesis of UC.
Das
K M
KM
Department of Gastroenterology & Hepatology, UMDNJ-Robert Wood Johnson Medical School, New Brunswick 08903.
Sakamaki
S
S
Vecchi
M
M
D000911:Antibodies, Monoclonal; D000920:Antibody-Dependent Cell Cytotoxicity; D003093:Colitis, Ulcerative / Q000276:immunology*; D003106:Colon / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G; D008970:Molecular Weight; D009928:Organ Specificity; D011506:Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011506:Proteins