Modified method for peptide mapping of collagen chains using cyanogen bromide-cleavage of protein within polyacrylamide gels.
A highly efficient method for cyanogen bromide (CNBr)-mapping of collagen peptides is described. This method was developed based on polypeptide cleavage by CNBr within gel slices according to Barsh et al. [1981) Collagen Relat. Res. 1, 543-548). The proposed method has the following advantages: (i) Analysis of both radiolabeled and unlabeled collagens is possible; (ii) CNBr-cleavage of polypeptides is performed in gel pieces which contain individual bands; (iii) The peptide losses are minimized, offering a more complete analysis of collagens including the low molecular weight CNBr-peptides.
Sokolov
B P
BP
Institute of Medical Genetics, Academy of Medical Sciences, Moscow, USSR.
Sher
B M
BM
Kalinin
V N
VN
D003094:Collagen / Q000032:analysis*; D003488:Cyanogen Bromide / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D010449:Peptide Mapping / Q000379:methods*; D011347:Procollagen / Q000032:analysis; D012867:Skin / Q000032:analysis
D011347:Procollagen; D003094:Collagen; D003488:Cyanogen Bromide