A monoclonal anti-platelet antibody which recognizes p55 antigen.
A monoclonal antibody (MoAb) H229(IgGl) was obtained by fusion between SP2/0, mouse myeloma cell line and spleen cells from BC3Fl mice immunized with HEL, a erythroleukemia cell line. MoAb H229 precipitated a 55 kilodalton(KD) molecule in reduced condition. It reacted with platelets and megakaryocytes, but not with monocytes, granulocytes, lymphocytes, thymocytes, red blood cells (RBC), colony-forming units-granulocyte/monocyte (CFU-GM), and burst forming units- erythroid (BFU-E). These findings suggest that MoAb H229 reacts with platelets specifically. However, MoAb H229 did not inhibit platelet aggregation induced by ristocetin, ADP, epinephrine and collagen.
Takaishi
M
M
Murakami
Y
Y
Ishioka
S
S
Yamakido
M
M
Tsubokura
T
T
Fu
S M
SM
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001792:Blood Platelets / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D006801:Humans; D051379:Mice; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D010980:Platelet Membrane Glycoproteins