title: Fast detection of homogeneous chemiluminescent immunoassays with a sensitive photoplate.
abstract: Chemiluminescent immunoassays performed in microtitre plates can be detected with a sensitive instant photoplate. An apparatus was constructed for this purpose making use of whole polystyrene microtitre plates plates possible. One of the advantages of immunoassays using enzyme-enhanced chemiluminescence is the possibility for developing homogeneous immunoassays. An example is described for a solid-phase immunoassay for 17 beta-testosterone making use of non-specific energy transfer in combination with photoplate detection. A testosterone-peroxidase conjugate was used as label. Quantification was performed using image-analysis techniques. The assay was also performed with colorimetric substrates for comparison.
authors.affiliation: Laboratory for Clinical Chemistry, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: E H
authors.initials: EH
authors.lastname: Jansen
authors.forename: C A
authors.initials: CA
authors.lastname: Buskens
authors.forename: R H
authors.initials: RH
authors.lastname: van den Berg
mesh_terms: D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D013739:Testosterone / Q000032:analysis
chemical_list: D013739:Testosterone