More efficient excision repair of pyrimidine dimers in the specific DNA sequence than in the genome overall in goldfish cells.
Excision repair of pyrimidine dimers induced by 254 nm UV was examined in the genome overall and in a specific sequence containing a transfected gene for hygromycin B resistance, in RBCF-1 cells derived from a goldfish, by the use of UV endonuclease of Micrococcus luteus and alkaline agarose gel electrophoresis. More than 40% of dimers were removed from the specific sequence, while about 20% were removed from the genome overall, within 24 h after exposure to UV (2.5-7.5 J/m2).
Komura
J
J
Mitani
H
H
Shima
A
A
D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics*; D004260:DNA Repair; D004586:Electrophoresis; D006054:Goldfish; D011740:Pyrimidine Dimers; D014162:Transfection; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D004247:DNA