Amino acid analysis: determination of cysteine plus half-cystine in proteins after hydrochloric acid hydrolysis with a disulfide compound as additive.
All cysteine and cystine in a protein are derivatized during hydrolysis in hydrochloric acid containing 3,3'-dithiodipropionic acid. The resulting derivative can be separated from other amino acids and used for quantitation of cysteine plus half-cystine. A procedure is presented for accurate determination by ion exchange chromatography and postcolumn derivatization of all amino acids from acid hydrolysis of a protein, including the Cys-derivative.
Barkholt
V
V
Institute of Biochemical Genetics, University of Copenhagen, Denmark.
Jensen
A L
AL
D000596:Amino Acids / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003545:Cysteine / Q000032:analysis*; D003553:Cystine / Q000032:analysis*; D004220:Disulfides; D006851:Hydrochloric Acid; D006868:Hydrolysis; D009113:Muramidase; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins
D000596:Amino Acids; D004220:Disulfides; D010446:Peptide Fragments; D011506:Proteins; D003553:Cystine; D009113:Muramidase; D003545:Cysteine; D006851:Hydrochloric Acid