Pyruvate kinase inhibited by L-cysteine as a marker of tumorigenic human urothelial cell lines.
It was found that a decrease in electrophoretic mobility of pyruvate kinase (PK) isoenzyme, and an increase of the sensitivity of this enzyme to L-cysteine, were markers of immortalization and tumorigenic properties, respectively, in human urothelial cell lines characterized by different grades of transformation (TGr) in vitro.
Gumińska
M
M
Institute of Medical Biochemistry, N. Copernicus Academy of Medicine, Kraków, Poland.
Stachurska
M B
MB
Christensen
B
B
Tromholt
V
V
Kieler
J
J
Radzikowski
C
C
Duś
D
D
D014408:Biomarkers, Tumor; D002460:Cell Line; D002843:Chromatin / Q000302:isolation & purification; D003545:Cysteine / Q000494:pharmacology*; D004589:Electrophoresis, Disc; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D011770:Pyruvate Kinase / Q000032:analysis / Q000037:antagonists & inhibitors*
D014408:Biomarkers, Tumor; D002843:Chromatin; D007527:Isoenzymes; D011770:Pyruvate Kinase; D003545:Cysteine