title: The multicatalytic proteinase. Multiple proteolytic activities.
abstract: The multicatalytic proteinase is a high molecular weight nonlysosomal proteinase which has been isolated from a variety of mammalian tissues and has been suggested to contain several distinct catalytic sites. The enzyme degrades protein and peptide substrates and can cleave bonds on the carboxyl side of basic, hydrophobic, and acidic amino acid residues. The three types of activity have been referred to as trypsin-like, chymotrypsin-like, and peptidyl-glutamyl peptide bond hydrolyzing activities, respectively. All of these proteolytic activities are associated with a single band on native polyacrylamide gels. The pH optimum of the proteinase (pH 7.5-9.5) depends on the substrate. Using synthetic peptide substrates it was possible to demonstrate two distinct activities. Trypsin-like activity is inhibited at concentrations of the peptide aldehyde inhibitors leupeptin and antipain or of N-ethylmaleimide which have little or no effect on chymotrypsin-like activity. Results of mixed-substrate experiments also suggest that there are at least two distinct types of catalytic sites. All proteolytic activity is lost following dissociation by urea or by acid treatment. Polyclonal antibodies raised against the intact multicatalytic proteinase precipitate the complex but have little effect on its proteolytic activities.
authors.affiliation: Department of Biochemistry, University of Leicester, United Kingdom.
authors.forename: A J
authors.initials: AJ
authors.lastname: Rivett
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003546:Cysteine Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D007074:Immunoglobulin G; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000302:isolation & purification / Q000378:metabolism*; D009842:Oligopeptides; D046988:Proteasome Endopeptidase Complex; D051381:Rats; D013379:Substrate Specificity; D013816:Thermodynamics; D014508:Urea / Q000494:pharmacology
chemical_list: D007074:Immunoglobulin G; D009097:Multienzyme Complexes; D009842:Oligopeptides; D014508:Urea; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex