TGF-beta stimulates primary human skin fibroblast DNA synthesis via an autocrine production of PDGF-related peptides.
Transforming growth factor-beta (TGF-beta) stimulates DNA synthesis in human foreskin fibroblasts after a prolonged lag period as compared with other growth factors. The mechanism of induction of DNA synthesis appears to be dependent on the synthesis and secretion of PDGF-related proteins as antibodies which are specific for PDGF can block the TGF-beta-induced DNA synthesis. Other growth factors such as PDGF, EGF, or FGF do not induce the synthesis of these PDGF-related proteins. Additionally, TGF-beta treatment of human foreskin fibroblasts induces the expression of the PDGF A-chain gene but not the B-chain gene. This phenomenon appears to function in vivo, as subcutaneous injection of TGF-beta in rat skin induces the expression of the PDGF A-chain gene. These data suggest that TGF-beta may stimulate the growth of fibroblastic cells via an autocrine production of PDGF-related proteins.
Soma
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Department of Ophthalmology, University of South Florida College of Medicine, Tampa 33612.
Grotendorst
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D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D012867:Skin / Q000166:cytology*; D015291:Transforming Growth Factors / Q000494:pharmacology*
D010982:Platelet-Derived Growth Factor; D015291:Transforming Growth Factors; D004247:DNA