Trial comparison of mitochondrial cristae craters images obtained by the freeze-fracturing and ultrathin sections method.
In two-folded lamina of the mitochondrial cristae occurs in mitochondria of spermatocytes large areas of the inner and outer halves in freeze-fracturing technique morphological observations suggest that in mitochondrial membrane there exist "crater-like' structures with internal diameter of approximately 18 nm. A question has come up why no mention has so far been found in the literature of the appearance of similar structure in mitochondrial cristae in specimens in transmission electron microscope (TEM) observed. Thus comparison of our findings obtained by the freeze-fracturing (FF) method with those achieved by TEM was made.
Cieciura
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Department of Histology and Embryology, Military Academy of Medicine, Lódź, Poland.
Zdunowski
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P
Pieta
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D000818:Animals; D005614:Freeze Fracturing / Q000379:methods; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D008928:Mitochondria / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000648:ultrastructure