Effects of inhibitors and activators of protein kinase C on late erythroid progenitor (CFU-e) colony formation in vitro.
Protein kinase C (PKC) plays a central role in external signal transduction for many cell types. To examine the involvement of PKC in the control of erythropoiesis, we tested the effects of PKC inhibitors on in vitro colony formation by late erythroid progenitors (CFU-e) from normal and Friend virus-infected mice. Inhibitors of PKC and other kinases (H-7 and H-8) inhibited CFU-e at concentrations which inhibit PKC. HA1004, an inhibitor of the cyclic nucleotide-dependent kinases and a weak inhibitor of PKC, had little effect on CFU-e. In the absence of erythropoietin, a combination of phorbol ester and Ca++ ionophore significantly increased normal CFU-e. These results suggest PKC plays a role in the transduction of regulatory signals for the growth of CFU-e.
Jenis
D M
DM
Laboratory of Cell Biology, AMC Cancer Research Center, Denver, Colorado 80214.
Johnson
C S
CS
Furmanski
P
P
D000818:Animals; D003114:Colony-Forming Units Assay; D004789:Enzyme Activation; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D004920:Erythropoiesis / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000196:embryology; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
D011493:Protein Kinase C