Isolated soluble fractions from spleen cell culture supernatants induce tumor enhancement.
Supernatants of spleen cell culture from small-tumor-bearing mice, large-tumor-bearing mice, and large-tumor resected mice were fractionated by Sephadex G-100. The biological activity on tumor growth of all fractions was tested in vivo. It was found that only one fraction (MW: 220-250 kD) from spleen cell supernatants of small-tumor-bearing mice or large-tumor resected mice enhanced tumor growth. In spleen cell supernatants from large-tumor-bearing mice, two fractions had enhancing activity (MW: 220-250 and 100-10 kD). Thus, the surgical resection of large tumor induced disappearance of enhancing activity from the fraction of lower molecular weight.
Eiján
A M
AM
Research Department, Institute of Oncology, A. H. Roffo, Buenois Aires, Argentina.
Jasnis
M A
MA
Motta
A B
AB
Oisgold-Dagá
S
S
D000230:Adenocarcinoma / Q000032:analysis*; D000818:Animals; D007108:Immune Tolerance; D008325:Mammary Neoplasms, Experimental / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000032:analysis*; D014407:Tumor Cells, Cultured