Ultradian mitotic rhythms in culture of human sarcomatous bone cells originating from the patient before and after chemotherapy.
Bone sarcomatous cells derived from human malignant tumors were cultured. The mitotic index was recorded for 39 hr. When the cultured cells originated from patients with cancer disease before any chemotherapy, ultradian mitotic rhythms of a 6-9-hr period were detected, but in many cases only after a sensitive statistical analysis was performed. When the cultured cells originated from cancer patients undergoing chemotherapy, the mitotic index was decreased, and the amplitudes of the 6-9-hr component oscillations of the mitotic index were highly significantly increased. Damping and fading out of an ultradian mitotic rhythmicity was a bad prognostic portent in bone cancer. With reference to chemotherapy, the restored and amplified ultradian rhythmicity disclosed an appreciable antitumor effect and better survival prospects for the patient.
Petrovic
A
A
Unité de Recherches sur la Croissance des Cartilages, INSERM--U. 213, Faculté de Médecine, Strasbourg, France.
Oudet
C
C
Stutzmann
J
J
D001859:Bone Neoplasms / Q000188:drug therapy*; D006801:Humans; D008938:Mitosis; D012509:Sarcoma / Q000188:drug therapy*; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects