[Preparation of crystals of chicken cytosolic aspartate amino- transferase, suitable for high resolution x-ray structural analysis].
The crystals of cytosolic chicken aspartate aminotransferase were grown from polyethylene glycol solutions. Two of the four crystal modifications obtained diffract to 1.8 A resolution. The crystals of the free holoenzyme belong to space group P2(1)2(1)2(1) with unit cell dimensions of a = 56.9, b = 126.9, c = 124.6 A. The crystals of the enzyme-maleate complex belong to the same space group with slightly different unit cell dimensions of a = 56.5, b = 126.1, c = 124.6 A. The influence of ions of several divalent metals, dioxane and non-ionic detergent beta-octylglucoside on crystallization have been investigated. The best crystals were obtained in the presence of Mg2+ ions. These crystals were used for data collection on the diffractometer.
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D000818:Animals; D001219:Aspartate Aminotransferases / Q000302:isolation & purification*; D002412:Cations; D002645:Chickens; D003460:Crystallization; D003600:Cytosol / Q000201:enzymology*; D003902:Detergents; D005960:Glucosides; D008670:Metals; D011487:Protein Conformation; D014961:X-Ray Diffraction
D002412:Cations; D003902:Detergents; D005960:Glucosides; D008670:Metals; C018619:octyl-beta-D-glucoside; D001219:Aspartate Aminotransferases
Poluchenie kristallov tsitozol'noĭ aspartataminotransferazy kur, prigodnykh dlia rentgenostrukturnogo issledovaniia pri vysokom razreshenii.