A simple and rapid method to evaluate lymphocyte response to mitogens.
A new method to evaluate lymphocyte response to mitogens is proposed. The method is based on the observation in fluorescence of the stimulated cells after their supravital staining on slides prestained with acridine orange in alcoholic solution. The results are expressed as Blast Index (IB). The method is very practical, easy to reproduce and shows a good correlation with 3H-thymidine uptake.
Giorgio
A
A
Istituto di Richerche Biologiche F. Redi Avellino, Italy.
Rambaldi
M
M
Iaquinto
G
G
Carrato
F
F
Ambrosone
L
L
Moles
D A
DA
Troise
C
C
D000165:Acridine Orange; D006801:Humans; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology*
D008934:Mitogens; D000165:Acridine Orange