[Influence of the intensity of oxygen mass transfer on the biosynthesis of amphotericin B].
Influence of oxygen mass transfer intensity characterized by the rate of oxygen dissolution (S) and the agitation rate (n), as well as influence of dissolved oxygen concentration on the process of amphotericin B biosynthesis was studied. It was shown that S = 40 and 110 mg/l. min and n = 450 and 800 min-1 were respectively the lower and the upper levels of the optimal conditions by oxygen mass transfer during amphotericin B biosynthesis. When biosynthesis of amphotericin B was conducted under conditions of the optimal oxygen mass transfer, the dissolved oxygen concentration of about 12 to 15 per cent of the saturation level was critical for the culture respiration. Inhibition of the culture respiration and antibiotic synthesis was induced under conditions of increased oxygen mass transfer intensity (S greater than 110 mg/l. min and n greater than 800 min-1) by high intensity mechanical agitation of the fermentation broth. Under conditions of decreased oxygen mass transfer (S less than 40 mg/l. min and n = less than 450 min-1) it was induced by insufficient supply of oxygen to the culture. On the basis of the results it was shown possible to control the aeration and agitation conditions by the rate of oxygen uptake and dissolved oxygen concentration. The data should be considered in optimization of aeration and agitation conditions in biosynthesis of amphotericin B in large fermenters.
Vekshin
G A
GA
Malkov
M A
MA
D000666:Amphotericin B / Q000096:biosynthesis*; D003470:Culture Media; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D013302:Streptomyces / Q000378:metabolism
D003470:Culture Media; D000666:Amphotericin B; D010100:Oxygen
Vliianie intensivnosti massoperedachi kisloroda na biosintez amfoteritsina B.