Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody.
Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (VK, JK, VH, JH) as do anti-azophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of beta-octylglucoside as tetragonal bipyramids in the space group of either P4(1)2(1)2 or P4(3)3(1)2 and with unit cell dimensions of a = b = 77.9 A, and c = 146.7 A. They diffract X-rays to better than 2.7 A resolution. Data up to 2.7 A resolution have been collected.
Lin
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SF
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Coll
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Gefter
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ML
Margolies
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MN
Wang
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AH
D000818:Animals; D000911:Antibodies, Monoclonal; D001391:Azo Compounds / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments; D007135:Immunoglobulin Variable Region; D051379:Mice; D008815:Mice, Inbred Strains; D014961:X-Ray Diffraction; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001391:Azo Compounds; D007140:Immunoglobulin Fab Fragments; D007135:Immunoglobulin Variable Region; D010132:p-Azobenzenearsonate