title: Triacylglycerol synthesis in cultured rat hepatocytes. The rate-limiting role of diacylglycerol acyltransferase.
abstract: The limiting role of diacylglycerol acyltransferase with respect to triacylglycerol synthesis in cultured rat hepatocytes was evaluated by following the inhibition of the overall synthetic flux by 2-bromooctanoate acting as an inhibitor of the diacylglycerol acyltransferase step. The flux-control coefficient of diacylglycerol acyltransferase in intact cultured hepatocytes amounted to 0.76 in the presence of saturating glycerol and either palmitate or oleate as the fatty acyl substrates. The flux-control coefficient of diacylglycerol acyltransferase in lysolecithin-permeabilized cultured hepatocytes amounted to 0.80 and 0.99 in the presence of saturating glycerol 3-phosphate and either palmitate or oleate as the fatty acyl substrate, respectively. Hence, triacylglycerol synthesis in liver cells under the experimental conditions employed is rate-limited by the diacylglycerol acyltransferase.
authors.affiliation: Department of Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Mayorek
authors.forename: I
authors.initials: I
authors.lastname: Grinstein
authors.forename: J
authors.initials: J
authors.lastname: Bar-Tana
mesh_terms: D000214:Acyl Coenzyme A / Q000494:pharmacology; D000217:Acyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002210:Caprylates / Q000494:pharmacology; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D051048:Diacylglycerol O-Acyltransferase; D007700:Kinetics; D008066:Lipolysis / Q000187:drug effects; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008244:Lysophosphatidylcholines; D051381:Rats; D014280:Triglycerides / Q000096:biosynthesis*
chemical_list: D000214:Acyl Coenzyme A; D002210:Caprylates; D008244:Lysophosphatidylcholines; D014280:Triglycerides; C019279:2-bromooctanoic acid; C060151:2-bromooctanoyl-coenzyme A; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase