title: High voltage electric pulses efficiently induce fusion of cells in monolayer culture.
abstract: High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency of 3 X 10(-4) - 2 X 10(-5) after culturing fused cells in a selection medium that permits the growth of only hybrid cells. This method of electrofusion was applied for complementation analysis of DNA repair-deficient xeroderma pigmentosum cells. Efficient cell fusion was also observed with these human diploid fibroblasts and the resulting heterodikaryons showed a recovery of ultraviolet light-induced unscheduled DNA synthesis to the same extent as in normal cells.
authors.affiliation: Radiation Biology Center, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ishizaki
authors.forename: H R
authors.initials: HR
authors.lastname: Chang
authors.forename: T
authors.initials: T
authors.lastname: Eguchi
authors.forename: M
authors.initials: M
authors.lastname: Ikenaga
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D004560:Electricity; D005816:Genetic Complementation Test; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology* / Q000378:metabolism; D014983:Xeroderma Pigmentosum / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D004247:DNA