[Cell kinetic effects of cis-platinum on cancer cells in a culture system and in cases of cervical cancer using monoclonal antibody of BrdU].
Flow cytometric analyses were done in cervical squamous cell carcinoma cell line (SKG-IIIa) and in cancer cells of cervical cancer cases treated with CDDP using monoclonal antibody of BrdU. 1) In fundamental studies using culture system, accumulation of late S phase cells and of mid S phase cells was observed after 48 hours at the dose of 0.2 micrograms/ml and 0.5 micrograms/ml of CDDP, respectively. 2) Accumulation of early S phase cells was observed at the condition of 1 microgram/ml and 3 micrograms/ml of CDDP. All S phase cells decreased gradually thereafter 3) In clinical studies, accumulation of early S phase cells was observed in cancer cells of cervical cancer cases after intra-arterial infusion of CDDP. However, cell cycle recycling was observed in tumor cells which retained their low sensitivity to CDDP after 21-day treatment with intra-arterial infusion of CDDP.
Izutsu
T
T
Dept. of Obstetrics and Gynecology, Iwate Medical University.
Hirama
T
T
Matsuda
M
M
Yoshizumi
N
N
Kagabu
T
T
Nishiya
I
I
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000503:physiopathology; D002453:Cell Cycle / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007399:Interphase / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000503:physiopathology*
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine; D002945:Cisplatin