Modeling sickle cell vasoocclusion in the rat leg: quantification of trapped sickle cells and correlation with 31P metabolic and 1H magnetic resonance imaging changes.
We have developed an animal model to elucidate the acute effects of perfusion abnormalities on muscle metabolism induced by different density-defined classes of erythrocytes isolated from sickle cell anemia patients. Technetium-99m (99mTc)-labeled, saline-washed normal (AA), homozygous sickle (SS), or high-density SS (SS4) erythrocytes were injected into the femoral artery of the rat and quantitative 99mTc imaging, 31P magnetic resonance spectroscopy by surface coil at 2 teslas, and 1H magnetic resonance imaging at 0.15 tesla were performed. Between 5 and 25 microliters of SS4 cells was trapped in the microcirculation of the thigh (or 1-6 x 10(7) cells per cubic centimeter of tissue). In contrast, fewer SS discocytes (SS2) or AA cells were trapped (an equivalent packed cell volume of less than 6.7 microliters and 0.3 microliters, respectively). After injection of SS4 cells an initial increase in inorganic phosphate was observed in the region of the thigh served by the femoral artery, intracellular pH decreased, and subsequently the proton relaxation time T1 reached a broad maximum at 18-28 hr. When T1 obtained at this time was plotted against the volume of cells trapped, an increase of T1 over the control value of 411 +/- 48 msec was found that was proportional to the number of cells trapped. We conclude that the densest SS cells are most effective at producing vasoocclusion. The extent of the change detected by 1H magnetic resonance imaging is dependent on the amount of cells trapped in the microcirculation and the magnitude of the initial increase of inorganic phosphate.
Fabry
M E
ME
Department of Medicine, Albert Einstein College of Medicine, Bronx, NY.
Rajanayagam
V
V
Fine
E
E
Holland
S
S
Gore
J C
JC
Nagel
R L
RL
Kaul
D K
DK
D000755:Anemia, Sickle Cell / Q000097:blood*; D000818:Animals; D004195:Disease Models, Animal; D004734:Energy Metabolism; D004913:Erythrocytes, Abnormal / Q000503:physiopathology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007866:Leg / Q000098:blood supply; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008833:Microcirculation; D010725:Phosphocreatine / Q000502:physiology; D051381:Rats; D014652:Vascular Diseases / Q000503:physiopathology*
D010725:Phosphocreatine