title: Interactions between native oat phytochrome and tetrapyrroles.
abstract: The suggestion, that the increase in the far-UV CD signal of the 124 kDa oat phytochrome upon phototransformation of the Pr to Pfr form is possibly due to the chromophore interaction with the N-terminus segment of the phytochrome protein in the Pfr from (Chai, Y.G., Song, P.S., Cordonnier, M.-M. and Pratt, L.H. (1987) Biochemistry 26, 4947-4952), has been investigated by measuring the circular dichroism in the absence of exogenous tetrapyrrolic chromophores (bilirubin, biliverdin, chlorophyllin and hemin). Open tetrapyrrolic chromophores (bilirubin and biliverdin) did not have any significant effect on the phototransformability of the far-UV CD signal of the phytochrome, whereas closed tetrapyrroles (chlorophyllin and hemin) almost completely blocked the increase in the far-UV CD signal upon Pr to Pfr phototransformation. However, closed tetrapyrroles had no effect on the decrease in the CD signal upon Pfr to Pr photoconversion. Secondary structure analysis showed that the alpha-helix content of both Pr and Pfr forms of phytochrome (with 53 and 56% alpha-helical content, respectively) increased to 62% when a 50-fold molar excess of chlorophyllin was added to them separately. Spectral phototransformation of phytochrome was not affected in the presence of tetrapyrroles, except in the case of hemin. A 50-fold molar mass of hemin caused a significant bleaching of the Pfr form of phytochrome but not that of the Pr form. These results suggest that the chromophore-protein interaction is significantly altered during the phototransformation of phytochrome.
authors.affiliation: Department of Chemistry, School of Biological Sciences, University of Nebraska, Lincoln 68588.
authors.forename: B R
authors.initials: BR
authors.lastname: Singh
authors.forename: P S
authors.initials: PS
authors.lastname: Song
mesh_terms: D001663:Bilirubin; D001664:Biliverdine; D055598:Chemical Phenomena; D002621:Chemistry; D002735:Chlorophyllides; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D002523:Edible Grain; D006427:Hemin; D066298:In Vitro Techniques; D008027:Light; D010834:Phytochrome / Q000528:radiation effects / Q000648:ultrastructure; D010940:Plant Proteins / Q000528:radiation effects / Q000648:ultrastructure; D011487:Protein Conformation; D011758:Pyrroles; D045725:Tetrapyrroles
chemical_list: D002735:Chlorophyllides; D010940:Plant Proteins; D011758:Pyrroles; D045725:Tetrapyrroles; D010834:Phytochrome; D006427:Hemin; C007020:chlorophyllin; D001664:Biliverdine; D001663:Bilirubin