title: Crystallization of a new class of microbial ribonuclease from Rhizopus niveus.
abstract: Crystals of ribonuclease Rh, a new class of microbial ribonuclease from Rhizopus niveus, were obtained from polyethylene glycol 8000 solution by a vapour diffusion technique in the hanging drop mode. Two crystal forms, type I and type II, were obtained from the same droplet solution. Both forms belong to the space group P2(1)2(1)2(1), but their cell dimensions are markedly different: a = 68.3 A, b = 73.0 A, c = 50.0 A for type I and a = 67.5 A, b = 72.3 A, c = 44.2 A for type II. The type I crystals diffract beyond 2.0 A resolution and are suitable for X-ray structure analysis at high resolution.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kurihara
authors.forename: Y
authors.initials: Y
authors.lastname: Mitsui
authors.forename: K T
authors.initials: KT
authors.lastname: Nakamura
authors.forename: E
authors.initials: E
authors.lastname: Wakabayashi
authors.forename: K
authors.initials: K
authors.lastname: Ohgi
authors.forename: M
authors.initials: M
authors.lastname: Irie
mesh_terms: D003460:Crystallization; D012233:Rhizopus / Q000201:enzymology*; D012260:Ribonucleases; D014961:X-Ray Diffraction
chemical_list: D012260:Ribonucleases