Demonstration of distinct agonist and antagonist conformations of the A1 adenosine receptor.
A1 adenosine receptor-binding subunits can be visualized using high affinity antagonist and agonist photoaffinity radioligands. In the present study, we examined whether agonists and antagonists bind to the same receptor-binding subunit and if agonists and antagonists induce different conformational states of the receptor in intact membranes. It was demonstrated that several agonist and antagonist photoaffinity receptor-binding subunit. When the agonist and antagonist photoaffinity labeled peptides were denatured and subjected to partial peptide map analysis using a two-dimensional gel electrophoresis system similar peptide fragments were generated from each specifically labeled protein. This suggests that both classes of ligand label and incorporate into the same binding subunit. Proteolytic digestions of agonist- and antagonist-occupied receptors in native intact membranes revealed distinct and different peptide fragments depending on whether the ligand was an agonist or an antagonist. Manipulation of incubation conditions to perturb ligand-receptor interactions alter the pattern of peptide fragments generated with each specific protease. These data suggest that agonist and antagonist photoaffinity probes interact with an incorporate into the same binding subunit but that agonist binding is associated with a unique and detectable receptor conformation.
Barrington
W W
WW
Department of Medicine (Cardiology), Duke University Medical Center, Durham, North Carolina 27710.
Jacobson
K A
KA
Stiles
G L
GL
D000345:Affinity Labels; D000818:Animals; D001386:Azides; D001921:Brain / Q000378:metabolism; D002417:Cattle; D007457:Iodine Radioisotopes; D010449:Peptide Mapping; D010777:Photochemistry; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation; D011983:Receptors, Purinergic / Q000187:drug effects / Q000378:metabolism
D000345:Affinity Labels; D001386:Azides; D007457:Iodine Radioisotopes; D011983:Receptors, Purinergic; C050003:N(6)-2-(4-azido-3-iodophenyl)ethyladenosine