title: More efficient excision repair of pyrimidine dimers in the specific DNA sequence than in the genome overall in goldfish cells.
abstract: Excision repair of pyrimidine dimers induced by 254 nm UV was examined in the genome overall and in a specific sequence containing a transfected gene for hygromycin B resistance, in RBCF-1 cells derived from a goldfish, by the use of UV endonuclease of Micrococcus luteus and alkaline agarose gel electrophoresis. More than 40% of dimers were removed from the specific sequence, while about 20% were removed from the genome overall, within 24 h after exposure to UV (2.5-7.5 J/m2).
authors.forename: J
authors.initials: J
authors.lastname: Komura
authors.forename: H
authors.initials: H
authors.lastname: Mitani
authors.forename: A
authors.initials: A
authors.lastname: Shima
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics*; D004260:DNA Repair; D004586:Electrophoresis; D006054:Goldfish; D011740:Pyrimidine Dimers; D014162:Transfection; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers; D004247:DNA