[The effect of high hydrostatic pressure on the cell center and microtubules in tissue culture cells].
A network of cytoplasmic microtubules in PE cells disassembles at 37 degrees C under 1000 atm pr. in 12 to 14 hours; under 2000 atm pr., the disassembly time is not more than 2 hours. The reconstitution process sets in 20 minutes after pressure dropping to proceed diffusely throughout the cytoplasm. Microtubules attached to the cell center reappear in 45 minutes. The dynamics of microtubular disassembly and reconstitution indicates a complete inactivation of the cell center as a microtubule-organizing center.
Vorob'ev
I A
IA
Drachev
V A
VA
D000818:Animals; D046508:Culture Techniques / Q000295:instrumentation; D003593:Cytoplasm / Q000648:ultrastructure*; D006874:Hydrostatic Pressure; D007399:Interphase; D008870:Microtubules / Q000648:ultrastructure*; D008938:Mitosis; D013997:Time Factors
Vliianie vysokogo gidrostaticheskogo davleniia na kletochnyĭ tsentr i mikrotrubochki v kletkakh kul'tury tkani.