Ultraviolet shadowing nucleic acids on nylon membranes.
We describe a method for the direct visualization of nucleic acids on nylon membranes. Nylon is weakly fluorescent under short wave ultraviolet light allowing membrane-bound nucleic acids to be detected with a sensitivity of 10 ng. This procedure involves no staining or destaining of the gels prior to transfer, does not require duplicate sample lanes or blots, and does not interfere with transfer of the nucleic acid to the membrane or subsequent hybridization.
Thurston
S J
SJ
Jackson Laboratory, Bar Harbor, Maine 04609.
Saffer
J D
JD
D004587:Electrophoresis, Agar Gel; D009696:Nucleic Acids / Q000032:analysis*; D009757:Nylons / Q000528:radiation effects; D014466:Ultraviolet Rays
D009696:Nucleic Acids; D009757:Nylons