Binding of radiolabeled estrogens by human cells in vitro: implications to the development of a new diagnostic and therapeutic modality in the treatment of malignancies with estrogen receptors.
An in vitro technique was employed to demonstrate the binding of Auger electron-emitting nuclide-labeled estrogenic compounds by a variety of human cell types. Human granulosa cells, endometrium, and MCF-7 breast cancer cells were incubated with either 16 alpha-[I125]-iodoestradiol-17 beta or 16 alpha-[I123]-iodoestradiol-17 beta in vitro. Autoradiographic techniques were subsequently utilized and revealed that binding of these estrogenic compounds by all three types of cells did occur and that this binding was inhibited by excess unlabeled estradiol. Histologic examination was not able to demonstrate nuclear-specific binding in all instances, however. These compounds are potentially useful in both diagnostic and therapeutic settings and this study is the first to provide such data from human tissue.
Anderson
R E
RE
Department of Obstetrics and Gynecology, University of Chicago, Chicago Lying-In Hospital, Illinois 60637.
Holt
J A
JA
D001345:Autoradiography; D001943:Breast Neoplasms / Q000378:metabolism; D002478:Cells, Cultured; D004717:Endometrium / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D009369:Neoplasms / Q000175:diagnosis / Q000628:therapy; D011960:Receptors, Estrogen / Q000378:metabolism*
D007457:Iodine Radioisotopes; D011960:Receptors, Estrogen; D004958:Estradiol