title: A continuous-flow automated assay for iodometric estimation of hydroperoxides.
abstract: An iodometric method for the analysis of hydroperoxides has been automated to allow analysis of aqueous biological samples (containing less than 20 mg/ml protein) and lipid hydroperoxide extracts. The evolution of triiodide ions is measured spectrophotometrically at 360 nm. Dependent on the type of sample, 30-60 samples can be analyzed per hour and the system allows detection of less than 100 pmol of peroxide. The assay is linear over a range of 100 pmol to 25 nmol. The sample volume used routinely was 80 microliters.
authors.affiliation: Department of Biology/Biochemistry, Brunel University, Uxbridge, Middlesex, England.
authors.forename: S M
authors.initials: SM
authors.lastname: Thomas
authors.forename: W
authors.initials: W
authors.lastname: Jessup
authors.forename: J M
authors.initials: JM
authors.lastname: Gebicki
authors.forename: R T
authors.initials: RT
authors.lastname: Dean
mesh_terms: D000818:Animals; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D007455:Iodine / Q000032:analysis*; D007700:Kinetics; D008054:Lipid Peroxides / Q000032:analysis; D008077:Lipoproteins, LDL / Q000302:isolation & purification; D008081:Liposomes / Q000302:isolation & purification; D008928:Mitochondria / Q000032:analysis; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions
chemical_list: D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D008081:Liposomes; D010713:Phosphatidylcholines; D012996:Solutions; D007455:Iodine; D006861:Hydrogen Peroxide