Cytotoxicity of alkyl-2-cyanoacrylate adhesives.
The cytotoxicity of methyl- and isobutyl-2-cyanoacrylate adhesives was determined using a rat polymorphonuclear leukocyte suspension. Cell degranulation increased and migration decreased on addition of the alkyl-2-cyanoacrylates to the suspension in a concentration-dependent manner. When acetylsalicylic acid or indomethacin, inhibitors of prostaglandin H synthase, were present, the cytotoxicity observed on addition of the adhesives to the leukocytes decreased up to eightfold in a dose-dependent manner as detected by trypan blue exclusion. Likewise, glutathione peroxidase and superoxide dismutase lowered such cytotoxicity resulting from the cyanoacrylates up to eight- and sevenfold, respectively. The data suggested that the adhesives may have generated lipid hydroperoxides that activated prostaglandin and thromboxane biosynthesis, and participated in membranal oxidation and lysis. Such a mechanism may contribute to understanding the thrombotic events associated with the necrosis observed on application of these adhesives to tissues in vivo.
Papatheofanis
F J
FJ
Department of Orthopaedics, University of Illinois, College of Medicine, Chicago.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003487:Cyanoacrylates / Q000633:toxicity*; D005260:Female; D009504:Neutrophils / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D014014:Tissue Adhesives / Q000633:toxicity*
D003487:Cyanoacrylates; D014014:Tissue Adhesives