Differential effects of acute and repeated electrically and chemically induced seizures on [3H]Nimodipine and [125I]omega-conotoxin GVIA binding in rat brain.
[3H]Nimodipine and high-affinity [125I]omega-conotoxin GVIA (CgTX) binding were investigated in membranes from rat cerebral cortex, cerebellum, and hippocampus after electrically and chemically induced seizures. Animals were decapitated 30 min after a single electroconvulsive shock (ECS) or lidocaine-induced seizure and 24 h after the last of 10 once-daily ECS or six once-daily lidocaine-induced seizures. After a single ECS, [3H]nimodipine and [125I]CgTX binding sites decreased in cerebral cortex (by 10% and 17%, respectively). A downregulation of [3H]nimodipine binding sites in hippocampus occurred after single and repeated lidocaine-induced seizures (by 24% and 11%, respectively), whereas [125I]CgTX binding remained unaltered. An earlier report on changes in [3H]nitrendipine binding after chronic ECS in cortex and hippocampus was not confirmed.
Gleiter
C H
CH
Laboratory of Clinical Studies, D.I.C.B.R., National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland.
Cain
C J
CJ
Weiss
S R
SR
Post
R M
RM
Marangos
P J
PJ
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D004597:Electroshock; D006624:Hippocampus / Q000378:metabolism; D007457:Iodine Radioisotopes; D008012:Lidocaine; D008297:Male; D008978:Mollusk Venoms / Q000378:metabolism*; D009553:Nimodipine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000139:chemically induced / Q000209:etiology / Q000378:metabolism*; D014316:Tritium; D020866:omega-Conotoxin GVIA
D002121:Calcium Channel Blockers; D007457:Iodine Radioisotopes; D008978:Mollusk Venoms; D014316:Tritium; D009553:Nimodipine; D020866:omega-Conotoxin GVIA; D008012:Lidocaine