title: [Synthesis of 5'-phosphorylated oligodeoxyribonucleotides by the H-phosphonate method].
abstract: N,O-protected 2'-deoxyribonucleotide-3'-H-phosphonates and 2-(p-nitrophenylethyl)-H-phosphonate were prepared and used in solid-phase synthesis of 5'-phosphorylated DNA. H-Phosphonate condensation is performed with 1:2 ratio of P-component to activator (pivaloyl chloride). Protection groups were removed either by sequential treatment with conc. NH3 and DBU or by combination of these reagents.
authors.forename: S A
authors.initials: SA
authors.lastname: Filippov
authors.forename: D S
authors.initials: DS
authors.lastname: Esipov
authors.forename: S V
authors.initials: SV
authors.lastname: Kalinichenko
authors.forename: V N
authors.initials: VN
authors.lastname: Dobrynin
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D010766:Phosphorylation
chemical_list: D009838:Oligodeoxyribonucleotides
vernacular_title: Sintez 5'-fosforilirovannykh oligodezoksiribonukleotidov H-fosfonatnym metodom.