title: Mitochondrial structure revealed by high-resolution scanning electron microscopy.
abstract: Mitochondrial structure has been examined in three dimensions using high-resolution scanning electron microscopy in cells from rat liver, retina (photoreceptors and retinal pigment epithelium), and kidney (proximal convoluted tubular cells and podocytes). Tissues were prepared by aldehyde-osmium fixation and freeze cleavage using a cryoprotectant, followed by removal of the cytosol by immersion in a dilute osmium tetroxide solution. The microscope used (Hitachi S-570) was equipped with a secondary electron detector located in the column above the specimen, situated within the objective lens. Mitochondria in all tissues examined were found to have only tubular cristae, which in some instances could be seen to span the entire diameter of the organelle. The walls of the tubular cristae, when unfractured, were in contact with the inner mitochondrial membrane; and their lumens were open to the intermembranous space. We hypothesize that in cells of many, perhaps most tissues, mitochondrial cristae are not shelf-like but are, in fact, tubes which span the mitochondrial matrix and are continuous with the inner mitochondrial membrane at both ends.
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Toronto, Ontario, Canada.
authors.forename: P J
authors.initials: PJ
authors.lastname: Lea
authors.forename: M J
authors.initials: MJ
authors.lastname: Hollenberg
mesh_terms: D000818:Animals; D005260:Female; D007091:Image Processing, Computer-Assisted; D007425:Intracellular Membranes / Q000648:ultrastructure; D007672:Kidney Cortex / Q000166:cytology / Q000648:ultrastructure; D008099:Liver / Q000166:cytology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000648:ultrastructure*; D008930:Mitochondria, Liver / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000166:cytology / Q000648:ultrastructure