title: Radionuclide imaging of experimental atherosclerosis with nonspecific polyclonal immunoglobulin G.
abstract: The utility of nonspecific polyclonal IgG for external imaging of experimental atherosclerosis was tested in a series of rabbits after balloon catheter deendothelialization of the abdominal aorta. Following injection of 111In-IgG, 111In-Fc, or 111In-Fab serial images were recorded. In addition, several animals received 125I-low density lipoproteins [125I-LDL], or 125I human serum albumin [125I-HSA] as positive and negative controls. Forty-eight hours after injection of the radiolabeled proteins, the aortas were removed, divided into abdominal and thoracic regions, counted, and autoradiographed. The images acquired after injection of 111In-IgG and 111In-Fc, showed clear focal accumulation of radioactivity in the healing abdominal aorta. In contrast, the images obtained after injection of 111In-Fab did not show focal radionuclide accumulation. For 111In-IgG and 111In-Fc there were three to six times as many counts in the abdominal as in the thoracic aorta, while for 111In-Fab and 125I HSA, the abdominal and thoracic counts were nearly equal. The results suggest that radiolabeled IgG and Fc can be used to image experimental atherosclerosis.
authors.affiliation: Department of Radiology, Massachusetts General Hospital, Boston 02114.
authors.forename: A J
authors.initials: AJ
authors.lastname: Fischman
authors.forename: R H
authors.initials: RH
authors.lastname: Rubin
authors.forename: B A
authors.initials: BA
authors.lastname: Khaw
authors.forename: P B
authors.initials: PB
authors.lastname: Kramer
authors.forename: R
authors.initials: R
authors.lastname: Wilkinson
authors.forename: M
authors.initials: M
authors.lastname: Ahmad
authors.forename: M
authors.initials: M
authors.lastname: Needelman
authors.forename: E
authors.initials: E
authors.lastname: Locke
authors.forename: N D
authors.initials: ND
authors.lastname: Nossiff
authors.forename: H W
authors.initials: HW
authors.lastname: Strauss
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000000981:diagnostic imaging*; D001345:Autoradiography; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G / Q000008:administration & dosage*; D007205:Indium Radioisotopes; D008297:Male; D011817:Rabbits; D011877:Radionuclide Imaging
chemical_list: D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007205:Indium Radioisotopes