Effects of allyl sulfide, germanium and NaCl on the development of glutathion S-transferase P-positive rat hepatic foci initiated by diethylnitrosamine.
The modification potentials of allyl sulfide, germanium and NaCl on the development of preneoplastic hepatic enzyme altered foci were examined in an in vivo mid-term assay system. Rats were initially given a single dose (200 mg/kg), intraperitoneal injection of diethylnitrosamine (DEN) and, starting 2 weeks later, were treated with allyl sulfide, germanium and NaCl at a concentration of 0.5%, 0.05% and 5%, respectively, in the diet for 6 weeks and then killed. All rats were subjected to two-thirds partial hepatectomy at week 3. The modifying potential was scored by comparing the number and area per cm2 of induced glutathione S-transferase placental form-positive (GST-P+) foci in the liver with those of the corresponding control group given DEN alone. Allyl sulfide exerted an obvious inhibition of the development of GST-P+ foci as regards both number and area. On the contrary, germanium showed a significant increase in the number of GST-P+ foci, although the data for area were not altered. NaCl had no modifying effect on their development.
Jang
J J
JJ
Department of Anatomical Pathology, Korea Cancer Center Hospital, Seoul.
Cho
K J
KJ
Kim
S H
SH
D000498:Allyl Compounds; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003043:Cocarcinogenesis; D004052:Diethylnitrosamine; D005857:Germanium / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000201:enzymology; D008297:Male; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000201:enzymology; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology*; D013440:Sulfides / Q000494:pharmacology*
D000498:Allyl Compounds; D013440:Sulfides; D005857:Germanium; D004052:Diethylnitrosamine; D012965:Sodium Chloride; C038491:allyl sulfide; D005982:Glutathione Transferase