title: Uptake of colchicine, a microtubular system disrupting agent, by isolated rat hepatocytes.
abstract: The possible mechanism of hepatic uptake of colchicine (CLC), a microtubule system disrupting agent, was examined using isolated rat hepatocytes. The existence of a carrier-mediated active transport system for CLC was demonstrated. This transport system is saturable, is affected by metabolic inhibitors (dinitrophenol, KCN) and a SH-group blocker (p-hydroxymercuribenzoic acid but not N-ethylmaleimide), and is sensitive to temperature. Ouabain, an inhibitor of Na+, K+-ATPase, does not affect the transport system of CLC.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Wierzba
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: K
authors.initials: K
authors.lastname: Okudaira
authors.forename: T
authors.initials: T
authors.lastname: Iga
authors.forename: M
authors.initials: M
authors.lastname: Hanano
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003078:Colchicine / Q000493:pharmacokinetics* / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000494:pharmacology
chemical_list: D013438:Sulfhydryl Compounds; D003078:Colchicine