Determination of methotrexate in untreated body fluids by micellar liquid chromatography.
A fast and sensitive method for determination of the antineoplastic drug methotrexate in untreated serum and urine is described. Reversed-phase liquid chromatography with sodium dodecyl sulfate (SDS) micellar mobile phase has been used with direct sample injection. Changes in mobile phase variables such as SDS concentration and/or pH profoundly affected drug retention. Recovery was quantitative and the detection limit (90 nM for a 20 microL sample size) was below the range normally monitored. Multichannel UV detection provided strong evidence regarding peak purity and peak identity.
Palmisano
F
F
Guerrieri
A
A
Zambonin
P G
PG
Cataldi
T R
TR
D001826:Body Fluids / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D008727:Methotrexate / Q000032:analysis* / Q000097:blood / Q000652:urine; D013056:Spectrophotometry, Ultraviolet
D008727:Methotrexate