Evaluation of butylated hydroxytoluene as a cryopreservative added to whole or skim milk diluent for bull semen.
Effect of five concentrations of butylated hydroxytoluene on the percent motility and percent intact acrosomes was evaluated for bull sperm frozen and thawed in whole and skim milk diluents. Twelve ejaculates were frozen in .5-ml straws in moving nitrogen vapor after each ejaculate was split among the five butylated hydroxytoluene treatments within the two diluents. Prefreeze and postthaw percent motility and postthaw percent intact acrosomes were evaluated at 0 and 4 h. Before freezing, sperm motilities were similar in whole or skim milk diluents with and without butylated hydroxytoluene. Upon thawing, sperm motility was 10 percentage points higher in whole milk diluent containing .5 and .75 mM butylated hydroxytoluene than in samples without it. After 4 h, percentage motile sperm was similar among all whole milk treatments. Sperm motility was similar among all skim milk treatments at both 0 and 4 h. Percent intact acrosomes were similar among all treatments for each diluent. Oxidation in whole milk diluent stored at 4 degrees C for 1 wk was reduced in the presence of 1.0, 2.0, and 4.0 mM butylated hydroxytoluene.
Killian
G
G
Dairy Breeding Research Center, Pennsylvania State University, University Park 16802.
Honadel
T
T
McNutt
T
T
Henault
M
M
Wegner
C
C
Dunlap
D
D
D000177:Acrosome / Q000502:physiology; D000818:Animals; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002417:Cattle / Q000502:physiology*; D003451:Cryoprotective Agents; D005615:Freezing; D008297:Male; D008892:Milk; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects
D003451:Cryoprotective Agents; D002084:Butylated Hydroxytoluene