Identification and partial separation of three distinct 32-kDa calcium/phospholipid-regulated proteins from bovine spleen.
We have separated three distinct 32-kDa calcium/phospholipid-regulated proteins from bovine spleen, which we have designated as 32kDa-Ia, 32kDa-Ib and 32kDa-II. By one-dimensional peptide mapping and chromatographic behavior, the three proteins are distinct from each other. Gizzard 35kDa calcimedin antibody recognizes both 32kDa-Ia and 32kDa-II but not 32kDa-Ib. Anti-aorta endonexin II specifically reacts with 32kDa-II. Bovine lens endonexin antibody reacts with 32kDa-Ia and 32kDa-Ib, but not with 32kDa-II. These data suggest that the 32-kDa calcium/phospholipid-regulated proteins purified from various sources can be divided into three distinct classes of proteins.
Kobayashi
R
R
Department of Biochemistry, Akita University School of Medicine, Japan.
Tashima
Y
Y
D000818:Animals; D002135:Calcium-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D003429:Cross Reactions; D008970:Molecular Weight; D010449:Peptide Mapping; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D010743:Phospholipids / Q000378:metabolism*; D013154:Spleen / Q000032:analysis*
D002135:Calcium-Binding Proteins; D010743:Phospholipids; D010741:Phospholipases A