title: In vitro culture of mouse embryos in human amniotic fluid.
abstract: Human amniotic fluid was compared with Ham's F-10 culture medium as a possible alternative for use in in vitro fertilisation. The cleavage success of mouse embryos in human amniotic fluid (experimental group) was 92% compared with 86% in Ham's F-10 medium. It is concluded that human amniotic fluid is a viable alternative culture medium for mouse embryos.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Pretoria.
authors.forename: J C
authors.initials: JC
authors.lastname: Coetzee
authors.forename: C B
authors.initials: CB
authors.lastname: Sevenster
authors.forename: F L
authors.initials: FL
authors.lastname: Fourie
authors.forename: J V
authors.initials: JV
authors.lastname: van der Merwe
authors.forename: L A
authors.initials: LA
authors.lastname: Helberg
mesh_terms: D000653:Amniotic Fluid; D000818:Animals; D003470:Culture Media; D004622:Embryo, Mammalian / Q000502:physiology*; D005314:Embryonic and Fetal Development; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D051379:Mice
chemical_list: D003470:Culture Media