Effect of phosphoenolpyruvate and adenosine triphosphate on rabbit skeletal muscle after ischaemia: preliminary biochemical study.
The present study examined the effect of phosphoenolpyruvate (PEP) and adenosine triphosphate (ATP) on rabbit skeletal muscle flap survival after warm ischaemia. Two muscle flap models, rectus femoris pedicle flap and latissimus dorsi free flap, were subjected to a total ischaemia of 4 hours at 37 degrees C and 20 degrees C, respectively. Immediately prior to revascularisation, the muscles were infused with either Hanks' balanced salt solution (BSS) or Hanks' BSS containing 200 mumol PEP and 6.6 mumol ATP. Quantification of muscle damage was determined by measuring the plasma levels of creatinine kinase (CK), lactate dehydrogenase (LDH), lactate, potassium, and phosphate at 0, 2, 24, and 96 hours after revascularisation. Infusion of PEP/ATP compared with Hanks' BSS alone significantly decreased the efflux of CK in both rectus femoris (P less than 0.025) and latissimus dorsi muscles (P less than 0.05) and of LDH in the rectus femoris muscle (P less than 0.01). No significant changes were observed, however, for the plasma levels of lactate, potassium, and phosphate. From this study it was concluded that PEP and ATP partially protect skeletal muscle from ischaemia and reperfusion injury.
Khazanchi
R K
RK
Microsurgery Research Centre, St. Vincent's Hospital, Victoria, Australia.
Knight
K R
KR
Angel
M F
MF
Pederson
W C
WC
Coe
S A
SA
O'Brien
B M
BM
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D007261:Infusions, Intra-Arterial; D007511:Ischemia / Q000503:physiopathology*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D009132:Muscles / Q000098:blood supply / Q000187:drug effects* / Q000201:enzymology; D010728:Phosphoenolpyruvate / Q000494:pharmacology*; D011817:Rabbits; D015424:Reperfusion / Q000379:methods; D015427:Reperfusion Injury / Q000517:prevention & control; D013524:Surgical Flaps
D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase