[Serologic diagnosis of intestinal yersiniosis. The design of a stable erythrocyte preparation for the indirect hemagglutination reaction].
Nine types of erythrocyte diagnostica of serovars O3 and O9, differing in the methods of obtaining sensitins and the physical state of erythrocytes, were put on trial. The preparations were used for the titration of hyperimmune sera and blood sera obtained from about 500 healthy persons, 300 patients with Yersinia enteric infection and 300 patients with other diseases. Freeze-dried diagnostica, when compared with liquid ones, were found to be less sensitive, but more stable and specific. Sensitins isolated by the methods of Westphal ad Boivin showed the highest degree of specificity. The authors believe freeze-dried sheep red blood with activated Boivin's antigen adsorbed onto them to be the optimal preparation for use in the passive hemagglutination (PHA) test. The preparation was found to retain its serological activity for as long as 2-3 years. The titer 1:160 (1:200) in the PHA test is recommended as the minimal diagnostic indicator. Erythrocyte diagnosticum is more sensitive, specific and stable than bacterial one. Since 1984 dried Yersinia erythrocyte diagnostica (serovars O3 and O9) have gone into quantity production at the Leningrad Research Institute for Vaccines and Sera.
Koroliuk
A M
AM
Golovacheva
S N
SN
Dulatova
M V
MV
D000907:Antibodies, Bacterial / Q000032:analysis; D000941:Antigens / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D005612:Freeze Drying; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007114:Immunization; D007410:Intestinal Diseases / Q000175:diagnosis*; D015009:Yersinia Infections / Q000175:diagnosis*; D015008:Yersinia enterocolitica / Q000276:immunology
D000907:Antibodies, Bacterial; D000941:Antigens; D000942:Antigens, Bacterial; C012031:sensitin
Serologicheskaia diagnostika kishechnogo iersinioza. Konstruirovanie stabil'nogo éritrotsitarnogo preparata dlia reaktsii nepriamoĭ gemaggliutinatsii.