title: Adenylate degradation products release from the human myocardium during open heart surgery.
abstract: Purine degradation products were determined in the human heart coronary sinus effluent collected from patients undergoing cardiac surgery, during infusion of a cardioplegic solution. At the onset of cardiopulmonary bypass the mean concentrations of adenosine, inosine and hypoxanthine were 0.1, 0.5 and 0.3 mumol/l, respectively. Ischemic arrest leads to a progressive increase of the respective levels to 1.4 17.8 and 9.6 mumol/l after 60-80 min of ischemia. Xanthine concentration was undetectable (less than 0.2 mumol/l) throughout. A substantial urate release (20 mumol/l) was observed which decreased with the duration of ischemia. Xanthine oxidoreductase activity in human myocardium was found to be below the detection limit (0.1 mU/g wet weight). Thus, urate release represented wash out of urate which had accumulated in the tissue.
authors.affiliation: Department of Biochemistry, Academic Medical School, Debinki, Poland.
authors.forename: R T
authors.initials: RT
authors.lastname: Smoleński
authors.forename: A C
authors.initials: AC
authors.lastname: Składanowski
authors.forename: M
authors.initials: M
authors.lastname: Perko
authors.forename: M M
authors.initials: MM
authors.lastname: Zydowo
mesh_terms: D000241:Adenosine / Q000378:metabolism; D001021:Aortic Valve / Q000601:surgery; D006348:Cardiac Surgical Procedures; D002314:Cardioplegic Solutions / Q000032:analysis; D002315:Cardiopulmonary Bypass; D002851:Chromatography, High Pressure Liquid; D006330:Heart Defects, Congenital / Q000601:surgery; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007288:Inosine / Q000378:metabolism; D008943:Mitral Valve / Q000601:surgery; D009206:Myocardium / Q000378:metabolism*; D011687:Purines / Q000378:metabolism*; D014968:Xanthine Dehydrogenase / Q000378:metabolism
chemical_list: D002314:Cardioplegic Solutions; D007042:Hypoxanthines; D011687:Purines; D019271:Hypoxanthine; D007288:Inosine; D014968:Xanthine Dehydrogenase; D000241:Adenosine