Effect of absorption promoters in intranasal administration of human fibroblast interferon as a powder dosage form in rabbits.
The utility of the absorption promoters, sodium glycocholate (GC-Na), ethylenediamine dihydrochloride (EDTA-2Na), sodium caprylate (Cap-Na) and sodium salicylate (Sal-Na), in the intranasal administration of human fibroblast interferon-beta (HuIFN-beta) in rabbits was investigated. The optimal amount of added EDTA-2Na, Cap-Na and Sal-Na with respect to HuIFN-beta was examined for nasal absorption in the powder dosage form. Formulations of HuIFN-beta with GC-Na showed greatly enhanced intranasal HuIFN-beta absorption, as compared to the other absorption promoters. The results of a stability study on HuIFN-beta in homogenates of nasal mucosa suggested that GC-Na behaved as a hydrolysis inhibitor in the nasal mucosa and maintained the activity of HuIFN-beta.
Igawa
T
T
Maitani
Y
Y
Machida
Y
Y
Nagai
T
T
D000042:Absorption; D000281:Administration, Intranasal; D000818:Animals; D005079:Excipients; D006801:Humans; D007370:Interferon Type I / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D011208:Powders; D011817:Rabbits
D005079:Excipients; D007370:Interferon Type I; D011208:Powders