Screening for interindividual differences in radiosensitivity by means of the micronucleus assay in human lymphocytes.
The response of unstimulated peripheral lymphocytes to a single dose of 3 Gy of 137Cs gamma rays was analysed in blood samples from 30 donors by a conventional micronucleus assay and from 14 donors by the cytokinesis-block (CB) method. Significant interindividual variations could be detected for the baseline levels and for induced levels of micronuclei. An age effect could be demonstrated with the conventional method for the number of spontaneous MN, but not with the CB method. The corresponding numerical estimate was 3.4 +/- 1.3% increase per year. No such increase was apparent for induced frequencies. Provided that cell proliferation kinetics is reliably taken into account the micronucleus assay could be helpful for diagnosing potential radiosensitive individuals.
Huber
R
R
Institut für Strahlenbiologie, München-Neuherberg, Federal Republic of Germany.
Braselmann
H
H
Bauchinger
M
M
D000328:Adult; D000375:Aging / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D015162:Micronucleus Tests; D008875:Middle Aged; D011836:Radiation Tolerance; D012907:Smoking / Q000235:genetics