Isoelectric focusing of monoamine oxidase subtypes as identified by MAO inhibitors.
Monoamine oxidase from various human tissues from several individuals was labeled with [3H]pargyline and solubilized by means of Triton X-100 or Triton X-100 and urea. The specificity of the labeling was assessed using various selective, reversible and irreversible inhibitors as pharmacologic tools in competition experiments. The labeled material was submitted to isoelectric focusing on polyacrylamide gels according to one- and two-dimensional electrophoretic procedures and with fluorographic detection. While the differences in electrophoretic mobility of the two subtypes, MAO-A and MAO-B, could be replicated the subtypes showed identical although heterogeneous charges in isoelectric focusing. This contrasts with recent findings of clear differences in the primary structure of monoamine oxidase subtypes and thus needs further clarification.
Department of Psychiatry, School of Medicine, University of Wuerzburg, F.R.G.
J
J
Fritze
P
P
Koronakis
C
C
Konradi
P
P
Riederer
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003720:Densitometry; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D008995:Monoamine Oxidase / Q000032:analysis*; D008996:Monoamine Oxidase Inhibitors; D009419:Nerve Tissue Proteins / Q000032:analysis
D008996:Monoamine Oxidase Inhibitors; D009419:Nerve Tissue Proteins; D008995:Monoamine Oxidase