Formation of a phosphoramide mustard-nucleotide adduct that is not by alkylation at the N7 position of guanine.
The reaction of 2'-deoxyguanosine 3'-monophosphate with phosphoramide mustard resulted in the formation of several adducts. One of these adducts was formed by linking phosphoramide mustard to the phosphate group of 2'-deoxyguanosine 3'-monophosphate rather than by the generally accepted mechanism involving alkylation at the N7 position of guanine. This adduct served as an acceptor for the transfer of 32p from [gamma 32P]ATP by polynucleotide kinase and thus could be detected by the sensitive 32p-postlabeling assay.
Grace Cancer Drug Center, Roswell Park Memorial Institute, Buffalo, NY 14263.
A E
AE
Maccubbin
L
L
Caballes
G B
GB
Chheda
R F
RF
Struck
H L
HL
Gurtoo
D000477:Alkylating Agents; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003520:Cyclophosphamide / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D006150:Guanine Nucleotides / Q000378:metabolism*; D010752:Phosphoramide Mustards / Q000378:metabolism*
D000477:Alkylating Agents; D006150:Guanine Nucleotides; D010752:Phosphoramide Mustards; C030090:phosphoramide mustard; D003520:Cyclophosphamide; D004247:DNA