Sequential anti-idiotypes define reciprocal idiotopes on the same anti-DNA antibody.
Sequential anti-idiotypes have defined a pair of idiotypes with an idiotype-anti-idiotype relationship to each other in the variable region of the monoclonal anti-DNA antibody 16/6. A rabbit immunized with 16/6 made an antibody-1 (anti-Id-16/6R) and an antibody-2 (anti-anti-Id-16/6 or anti-anti-Id 16/6M). The latter bound to M-2, a monoclonal mouse anti-Id-16/6. M-2 was used to immunize a second mouse, which produced M-16. The variable region of M-16 mimics an antigenic surface on Id-16/6 and binds to a peptide contained within the first hypervariable region of the light chain of 16/6. The complementary idiotypes of 16/6 represent a reciprotope, an idiotype/anti-idiotype pair within the variable region of the same immunoglobulin molecule which may have functional importance.
Department of Medicine A, Hadassah University Hospital, Jerusalem.
Y
Y
Naparstek
C
C
Mackworth-Young
L
L
Breitman
R S
RS
Schwartz
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region; D010446:Peptide Fragments