Postprandial hyperlipemia inhibits platelet aggregation without affecting prostanoid metabolism.
The objective of this work was to characterize changes in platelet aggregability during postprandial hypertriglyceridemia with special emphasis on arachidonic acid metabolism. Ten healthy young men consumed 100 g fat after a fasting period of 12 hr. In-vitro platelet aggregation induced by ADP and collagen was measured at 0, 3, 5, and 9 hours after the fat intake. The major arachidonic acid metabolites, 12-hydroxyeicosatetraenoic acid (12-HETE), thromboxane A2 (TXA2), prostaglandin F2 alpha (PGF2a), and prostaglandin E2 (PGE2) produced during collagen-induced platelet activation were quantified by gas chromatography/mass spectrometry. A significant decrease in platelet aggregability induced by both ADP and collagen was detected during the postprandial hyperlipemia. No significant changes could be found in the prostanoid pattern of collagen activated platelets. There was no correlation between the degree of the inhibition of platelet aggregation and the relative or absolute increase of triglyceride-levels in the plasma during the postprandial hyperlipemia.
Institute of Medical Biochemistry, University of Graz, Austria.
J
J
Nimpf
E
E
Malle
B
B
Leopold
H
H
Wurm
G M
GM
Kostner
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000328:Adult; D001095:Arachidonic Acids / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D006949:Hyperlipidemias / Q000097:blood*; D008297:Male; D010743:Phospholipids / Q000097:blood; D010974:Platelet Aggregation; D011453:Prostaglandins / Q000097:blood; D013929:Thromboxane B2 / Q000097:blood; D014280:Triglycerides / Q000097:blood
D001095:Arachidonic Acids; D004041:Dietary Fats; D006893:Hydroxyeicosatetraenoic Acids; D010743:Phospholipids; D011453:Prostaglandins; D014280:Triglycerides; D013929:Thromboxane B2; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D002784:Cholesterol