Replication of measles virus in the human plasma cell leukemia-derived LICR-LON-HMy2 cell line.
Measles virus is usually grown in human or monkey fibroblast cells. We now show that LICR-LON-HMy2 (LL2) cells, a human plasma cell leukemia-derived line which grows in suspension culture, will permissively support replication of measles virus to an extent achievable with Vero cells. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of measles virions produced by LL2 cells showed a polypeptide pattern typical of measles virus. As well, measles virus-infected LL2 cells, like infected Vero cells, were found to secrete large amounts of virus hemagglutinin, but not other virus proteins. We thus conclude that LL2 cells can be effectively used to produce milligram amounts of measles virus and that virus-clarified culture medium from measles virus-infected LL2 cells is a potential source for purifying virus hemagglutinin.
Ziola
B
B
Department of Microbiology, University of Saskatchewan, Saskatoon, Canada.
Morhart
M
M
Gilbert
L
L
Karvonen
B
B
Chen
X P
XP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D006389:Hemagglutinins, Viral / Q000096:biosynthesis; D006801:Humans; D008459:Measles virus / Q000254:growth & development*; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology; D014776:Virus Cultivation / Q000379:methods*
D000911:Antibodies, Monoclonal; D006389:Hemagglutinins, Viral; D014764:Viral Proteins