Study of the lipophilic character of a series of beta-carbolines.
The lipophilic character of a series of beta-carbolines has been studied. The RM values were measured by means of a reversed-phase thin-layer chromatographic (TLC) technique and compared with the RM values obtained by high-performance TLC (HPTLC), the log k' obtained by high-performance liquid chromatography (HPLC), and the log P values. The best equation shows a very good linear relationship between our RM values and the classical log P values obtained using an octanol-water system. The choice of a pH of 13.0 for the TLC system allowed the measurement of the RM values of molecules in their non-ionized form. The deviations from the linear relationship shown by the RM(HPTLC) and log k' values of two compounds were due to the fact that both compounds were at least partially ionized at the pH of 7.0 at which the HPTLC and HPLC determinations were carried out.
Biagi
G L
GL
Istituto di Farmacologia, Università di Bologna, Italy.
Pietrogrande
M C
MC
Barbaro
A M
AM
Guerra
M C
MC
Borea
P A
PA
Forti
G C
GC
D002243:Carbolines / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer / Q000379:methods; D013056:Spectrophotometry, Ultraviolet
D002243:Carbolines