Biological monitoring of dichloropropene: air concentrations, urinary metabolite, and renal enzyme excretion.
Fifteen applicators of dichloropropene (DCP) were studied for personal air exposure to DCP, excretion of the metabolite of DCP (3CNAC), and excretion of the renal tubular enzyme, N-acetyl glucosaminidase (NAG). Each was studied for four 6-8 h consecutive intervals following baseline determinations of 3CNAC and NAG excretion. In accord with pilot data, 24-h urinary excretion of 3CNAC (mg) correlated well with exposure product for DCP (min exposed.mg/m3), r = 0.854, p less than .001. A more precise correlation of the air exposure product with urinary excretion of 3CNAC was discerned by using the morning urine after the previous day of exposure (micrograms/mg of creatinine), r = 0.914, p less than .001. Four workers had clinically elevated activity of NAG (greater than 4 mU/mg creatinine) in any of their urine collections after baseline. Nine workers showed greater than 25% increases in NAG excretion when compared to baseline. Dichloropropene air exposure products of greater than 700 mg.min/m3 or excretion of greater than 1.5 mg 3CNAC/d distinguished abnormally high daily excretion of NAG. These data demonstrate a firm positive relationship between air exposure and internal exposure, and a possible subclinical nephrotoxic effect in DCP workers.
Department of Laboratory Medicine and Medicine, University of California, San Francisco.
J D
JD
Osterloh
R
R
Wang
F
F
Schneider
K
K
Maddy
D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000652:urine; D000118:Acetylglucosaminidase / Q000652:urine*; D000395:Air Pollutants, Occupational / Q000032:analysis*; D000498:Allyl Compounds / Q000032:analysis* / Q000652:urine; D004781:Environmental Exposure; D008401:Gas Chromatography-Mass Spectrometry; D006596:Hexosaminidases / Q000652:urine*; D006801:Humans; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000032:analysis* / Q000652:urine; D007536:Isomerism; D007684:Kidney Tubules / Q000201:enzymology*; D008297:Male; D010865:Pilot Projects; D013997:Time Factors
D000395:Air Pollutants, Occupational; D000498:Allyl Compounds; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; C042828:N-acetyl-S-(3-chloroprop-2-enyl)cysteine; C023891:1,3-dichloro-1-propene; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D000111:Acetylcysteine