Identification of semen in stain by determination of the specific activity of L-tartrate-inhibitable acid phosphatase.
For identification of semen in stain the specific activity of L-tartrate-inhibitable acid phosphatase (ACP) was determined. With each stain extract, both enzyme activity and protein concentration were determined, and the specific activity (enzyme activity/protein concentration) was calculated. Seminal stains showed a value of 23.8 +/- 15.2 (mean +/- SD) IU/mg protein, while vaginal fluid stains showed a value of 0.088 +/- 0.049 IU/mg protein. Stains of other body fluids did not show any L-tartrate-inhibitable ACP activity. Furthermore, only eight of 30 plant juice stains showed any levels of L-tartrate-inhibitable ACP, although all plants tested showed ACP activity. As the present method enables us to analyze forensic samples quantitatively, it seems to be useful for forensic practice.
Dept. of Legal Medicine, Nagoya University School of Medicine, Japan.
K
K
Tamaki
K
K
Fujisawa
H
H
Okajima
K
K
Sato
Y
Y
Katsumata
D000135:Acid Phosphatase / Q000032:analysis / Q000037:antagonists & inhibitors*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008297:Male; D011902:Rape / Q000331:legislation & jurisprudence; D012661:Semen / Q000201:enzymology*; D013644:Tartrates / Q000494:pharmacology*
D013644:Tartrates; D000135:Acid Phosphatase; C029768:tartaric acid