The expression, localization, and effect of a human interferon in plants.
The ORF II of Cauliflower mosaic virus (CaMV) DNA was replaced with the human IFN alpha D coding sequence to yield a stable CaMV strain designated Ca524i. Inoculation of turnip (Brassica rapa cv "Just Right") with strain Ca524i DNA excised from plasmid pCa524i resulted in the production of biologically active IFN alpha D in infected plants. This was also true for its mutant (Ca562i) where one of the CYS codons was deleted. IFN alpha D produced in planta did not hamper superinfection with a single-stranded (+) sense RNA plant virus, turnip yellow mosaic virus (TYMV). Antibody gold labeling techniques and electron microscopy of infected plants showed that IFN was localized in the CaMV inclusion bodies.
Friederich Miescher-Institut, Basel, Switzerland.
G A
GA
De Zoeten
J R
JR
Penswick
M A
MA
Horisberger
P
P
Ahl
M
M
Schultze
T
T
Hohn
D002451:Cell Compartmentation; D003001:Cloning, Molecular; D005814:Genes, Viral; D005822:Genetic Vectors; D007150:Immunohistochemistry; D007181:Inclusion Bodies, Viral / Q000378:metabolism; D007370:Interferon Type I / Q000235:genetics* / Q000378:metabolism; D008854:Microscopy, Electron; D009029:Mosaic Viruses / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D011994:Recombinant Proteins; D014762:Viral Interference
D007370:Interferon Type I; D012367:RNA, Viral; D011994:Recombinant Proteins