New fluorescence assay for the quantitation of fungi.
Quantitative determination of fungal mass is easily achieved with a new procedure that detects particle epifluorescence. Fungi are detected after exposure to a fluorescent stain (Fungiqual; CIBA-GEIGY Corp., Summit, N.J.) by using a fluorescence particle concentration analyzer. This report describes a simple fluorescence method for quantitation of either yeast or mycelial forms of fungi. The nature of the staining reaction was studied, and a practical application of this procedure for determination of fungal susceptibility to an antifungal agent is presented.
Coleman
T
T
Infectious Diseases Division, Jewish Hospital, St. Louis, Missouri.
Madassery
J V
JV
Kobayashi
G S
GS
Nahm
M H
MH
Little
J R
JR
D000666:Amphotericin B / Q000494:pharmacology; D015169:Colony Count, Microbial; D005453:Fluorescence; D005456:Fluorescent Dyes; D005470:Fluorometry; D005658:Fungi / Q000187:drug effects / Q000254:growth & development*; D006801:Humans; D015394:Molecular Structure; D013267:Stilbenes; D014227:Triazines
D005456:Fluorescent Dyes; D013267:Stilbenes; D014227:Triazines; C061166:fungiqual; D000666:Amphotericin B