[Species specific motility patterns of hyperactivated mammalian spermatozoa and quantitative analysis of the hyperactivation of bull spermatozoa].
Movement tracks of spermatozoa of human, lion, tiger, cow, pig and sheep are recorded by dark field photography (fluid layer thickness 16.7 microns, exposure time 1 s). Comparison before and after capacitation by an incubation of 2 h in modified tissue culture medium TCM 199 with 10% fetal calf serum resulted in two quite different patterns of hyperactivated spermatozoa: 1) Tracks are broadened due to enlarged lateral head displacement or radius of rotating head movements respectively (tiger, lion) and show beside that a markedly increase in erratic motility (human). 2) In the studied species of artiodactyla, cow, pig and sheep, a qualitative new, panicle-like pattern arised as a result of superposition of spermatozoa head pendular movements around the axis of forward motility and the other one around the head axis. This new type of tracks allows a simple quantitative analysis of hyperactivation of bull spermatozoa, first described in this report. The method is applied to investigations on efficacy of capacitation media and provides evidence for high individual differences of semen donors in capacitation success.
Blottner
S
S
Akademie der Wissenschaften der DDR, Forschungsstelle für Wirbeltierforschung.
Bertram
R
R
Pitra
C
C
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D006801:Humans; D008045:Lions; D008297:Male; D010781:Photography; D012756:Sheep; D013045:Species Specificity; D013075:Sperm Capacitation; D013081:Sperm Motility; D013552:Swine
Speciesspezifische Motilitätsmuster hyperaktivierter Säugetierspermatozoen und die quantitative Auswertung der Hyperaktivierung von Bullenspermatozoen.