An in vivo study of benzene metabolite DNA adduct formation in liver of male New Zealand rabbits.
Rabbits were treated with benzene (586 mg/kg/b.i.d./4 days) after which DNA was isolated from liver and analyzed for adduct formation using the [32P] post-labeling method of Randerath and coworkers (Randerath et al. 1981; Reddy et al. 1984, 1986, 1987). Liver 500 g and 9000 g fractions were analyzed for adducts. There appeared to be several adducts in both the 500 g and 9000 g fractions observed on radioautographs of cellulose-TLC plates. Several adducts were also observed when the 9000 g fraction was studied using HPLC.
Bauer
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Joint Graduate Program in Toxicology, Rutgers the State University of New Jersey, Piscataway 08855.
Dimitriadis
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EA
Snyder
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R
D000818:Animals; D001554:Benzene / Q000378:metabolism* / Q000633:toxicity; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010761:Phosphorus Radioisotopes; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
D010761:Phosphorus Radioisotopes; D004247:DNA; D001554:Benzene