Natural cell-mediated cytotoxicity against syngeneic rat chondrocytes originating from different types of cartilage.
Natural anti-chondrocyte cytotoxicity of normal rat splenocytes, peritoneal cells and thymocytes was tested by means of 51Cr-release assay. Chondrocytes derived from epiphyseal, costal, nasal, and auricular cartilages were used as target cells. In some experiments, erythroleukaemic K-562 cells, known as typical natural killer cell targets, were also used. All types of chondrocytes were lysed equally well by splenocytes. Peritoneal cells exerted a low cytotoxic effect, whilst very low, almost negligible, cytotoxicity was noted with thymocytes. Negative selection with antibodies and complement showed that spleen-derived anti-chondrocyte effector cells are endowed with surface ganglioside asialo-GM1. A similar result was obtained in parallel experiments with K-562 cells. Moreover, 'cold' target experiments demonstrated that the release of 51Cr from the labelled chondrocytes could be inhibited by addition of unlabelled chondrocytes and K-562 cells.
Department of Histology and Embryology, Warsaw Medical School, Poland.
D M
DM
Radomska
A
A
Osiecka
J
J
Malejczyk
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D014407:Tumor Cells, Cultured