[Ultrastructural changes of platelets during ADP- stimulated aggregation (in the presence of fibrinogen)].
The ultrastructure of resting and stimulated human blood platelets (P) was studied by the transmission electron microscopy. The cells were chemically fixed (using tannic acid and OsFeCN mixture) 1, 3, 5 and 15 min after the addition of ADP and fibrinogen (F). Early changes in P ultrastructure consist in drastic reduction of the electron-dense layer of glycocalyx and in an increase of the plasma membrane permeability. At the early stages of P aggregation the cells contact with each other due to rapidly arising pseudopodia. Later, the extracellular network containing an exogenous F participates in the aggregation process.
Belitser
N V
NV
Anishchuk
M G
MG
Pozdniakova
T M
TM
Gorkun
O V
OV
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D001792:Blood Platelets / Q000648:ultrastructure*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D005340:Fibrinogen / Q000502:physiology*; D006801:Humans; D008854:Microscopy, Electron; D010974:Platelet Aggregation / Q000187:drug effects*
D000244:Adenosine Diphosphate; D005340:Fibrinogen
Ul'trastrukturnye izmeneniia trombotsitov pri ADF-stimuliruemoĭ agregatsii (v prisutstvii fibrinogena).