[Cytochemical and ultrastructural characteristics of the lining epithelium of the distal part of the epididymis in hamsters (Mesocricetus auratus)].
In the terminal segment of the hamster epididymidis there was some evidence of micro-merocrine protein secretion a the level of the principal cells and clear evidence of granular secretion in the light cells, presumable of glycoproteins. The PAS and protein cytochemistry reactivities observed in both these cells, of the ductus epithelial lining, but especially in the light cells, are suggestive of mucopolysaccharides and protein complexes synthesis and secretion. This secretion is carried out to the epididymal epithelium from the lumen and luminal content. A complex of small vacuoles and vesicles appeared to form from the Golgi complex is showed in the principal cells. It was suggested that this complex may represented merocrine secretory vacuoles and vesicles in these cells. Dense granules, at the TEM level, are observed in all the cytoplasm of the light cells, with correspondence to similar PAS-positive granules observed in these cells, at the light microscope level. These granules, at the TEM level, are actually secreted to the epididymal duct lumen, by the apical cytoplasms of the light cells. Signs of absorption were suggested to the principal and light columnar cells through the ultrastructural observations of micropinocytosis, apical multivesicular bodies or great membrane-bounded vacuoles in the adluminal cytoplasms.
C A
CA
Vicentini
A M
AM
Orsi
D000818:Animals; D006224:Cricetinae / Q000502:physiology*; D004822:Epididymis / Q000502:physiology / Q000648:ultrastructure*; D006025:Glycosaminoglycans / Q000378:metabolism; D008297:Male; D008647:Mesocricetus / Q000502:physiology*; D008867:Microtomy; D010503:Periodic Acid-Schiff Reaction
D006025:Glycosaminoglycans; C020341:glucosaminoglycans
Características citoquímicas e ultraestruturais do epitélio de revestimento na parte distal do epidídimo do hamster (Mesocricetus auratus).