Peptide histidine isoleucine (PHI) immunoreactivity in the rat retina: identification and characterisation by radioimmunoassay, immunohistochemistry and high-performance liquid chromatography.
The presence of peptide histidine isoleucine immunoreactivity (PHI-IR) in the retinae of male Wistar rats has been demonstrated using a specific radioimmunoassay (RIA), immunohistochemistry (IHC) and high performance liquid chromatography (HPLC). PHI levels (mean +/- S.E.M.) of 16.68 +/- 1.61 ng/g wet weight of normal retinal tissue were measured by RIA. IHC showed PHI-IR to be localized to a population of amacrine cells distributed along the proximal aspect of the inner nuclear layer and to processes in laminae I and IV of the inner plexiform layer. PHI-IR in retinal extracts was shown to coelute with a synthetic porcine PHI standard on a reverse phase HPLC system. Additionally levels of PHI-IR were significantly increased (P less than 0.001) with light adaptation.
Department of Medicine, Queen's University of Belfast, Northern Ireland.
P E
PE
Armstrong
W L
WL
Foy
C F
CF
Johnston
C
C
Shaw
R F
RF
Murphy
K D
KD
Buchanan
D000221:Adaptation, Ocular; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007150:Immunohistochemistry; D008297:Male; D010451:Peptide PHI / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000032:analysis*
D010451:Peptide PHI