Benoxaprofen inhibits the adhesion of human monocytes to cultured vascular endothelium.
Pretreatment of human monocytes with benoxaprofen for at least 2 h produced a dose-dependent abrogation of their adhesion to monolayers of cultured porcine endothelium with 0.05 microgram/ml and 50.0 micrograms/ml of the drug inducing a mean 33% and 83% inhibition of adhesion respectively. When the endothelium was treated with the drug there was no modification of monocyte adhesion. In contrast, pretreatment of endothelium with 5.0 and 50.0 micrograms/ml benoxaprofen for at least 6 h resulted in a mean 35% and 31% inhibition of polymorphonuclear cell (PMN) adhesion in 6/11 experiments. This inhibitory effect was not seen when drug-treated PMNs were added to endothelium. An impairment of monocyte chemotactic migration was only apparent with high concentrations of the drug (50 micrograms/ml). These results suggest that an important anti-inflammatory property of benoxaprofen is the inhibition of monocyte adhesion to vascular endothelium.
Department of Immunology, United Medical School, St. Thomas' Campus, London, UK.
K A
KA
Brown
J
J
Ferrie
B
B
Wilbourn
D C
DC
Dumonde
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects; D006801:Humans; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D011422:Propionates / Q000008:administration & dosage / Q000494:pharmacology*; D013552:Swine
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011422:Propionates; C011677:benoxaprofen