An homologous radioimmunoassay for coho salmon (Oncorhynchus kisutch) vitellogenin, with general applicability to other Pacific salmonids.
This paper describes an homologous radioimmunoassay for coho salmon vitellogenin that demonstrates parallel cross-reactivity for plasma vitellogenin of all Pacific salmonids tested (chinook, chum, coho, pink, and sockeye salmon, and cutthroat and rainbow trout), but not for Atlantic salmon or two nonsalmonids: common carp and sablefish. Plasma vitellogenin levels were high in ovulatory female Pacific salmonids (micrograms/ml to mg/ml range), but were mostly nondetectable in spermiating males of the same species.
Benfey
T J
TJ
Department of Fisheries and Oceans, West Vancouver Laboratory, British Columbia, Canada.
Donaldson
E M
EM
Owen
T G
TG
D000818:Animals; D003429:Cross Reactions; D005260:Female; D008297:Male; D011863:Radioimmunoassay / Q000379:methods*; D012474:Salmon / Q000378:metabolism*; D014819:Vitellogenins / Q000097:blood* / Q000276:immunology
D014819:Vitellogenins