Lectin histochemistry in ulcerative colitis and Crohn's disease.
The glycoconjugate composition of intestinal goblet cell mucin was characterized according to the anatomical distribution of lectin-binding sites in surgically resected intestinal tissues and mucosal biopsy specimens obtained from 38 control subjects, and from 32 patients with the active phase of ulcerative colitis, and 12 with Crohn's disease. Immunoperoxidase labeling studies found that in control tissues binding by Soybean Agglutinin (SBA), Dolichos Biflorus Agglutinin (DBA), Wheatgerm Agglutinin (WGA), and Ricinus Communis Agglutinin-120 (RCA-120) was consistently higher than that of Peanut Agglutinin (PNA), Ulex Europaeus Agglutinin-1 (UEA-1), Concanavalin A (ConA) and Helix Pomatia Agglutinin (HPA). Tissues from ulcerative colitis and Crohn's disease patients, showed increases in DBA and SBA binding, a reduction in HPA binding, and changes in the distribution of PNA, UEA-1, RCA-120, and HPA labeling sites. These results demonstrated that the expression of lectin-binding sites on human intestinal goblet mucin was specifically altered in ulcerative colitis and Crohn's disease, thus possibly providing another approach to the assessment of neoplastic risk on these diseases.
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
H
H
Yoshioka
M
M
Inada
K
K
Ogawa
G
G
Ohshio
H
H
Yamabe
Y
Y
Hamashima
T
T
Miyake
D000293:Adolescent; D000328:Adult; D000368:Aged; D003093:Colitis, Ulcerative / Q000378:metabolism*; D003208:Concanavalin A / Q000378:metabolism; D003424:Crohn Disease / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019887:Peanut Agglutinin; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins / Q000378:metabolism
C038095:Helix lectin; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; C036850:Ulex europaeus lectins; D014909:Wheat Germ Agglutinins; C036554:dolichos biflorus agglutinin; D003208:Concanavalin A