Evaluation of sulfonated aluminum phthalocyanines for use in photochemotherapy. A study on the relative efficiencies of photoinactivation.
The cellular photosensitivity caused by aluminum phthalocyanines sulfonated to different degrees (AlPcSn) has been investigated. The phototoxic effect increased with decreasing number of sulfonate groups on the macrocycle, with the exception of AlPcS1 which was less phototoxic than AlPcS2 but more phototoxic than AlPcS3 and AlPcS4. The tendency of the AlPcSns to aggregate in our cellular system increased with increasing lipophilicity of the sensitizers. The aggregates had little or no photosensitizing activity. The low efficiency of cell inactivation caused by AlPcS1 can be explained by the highly aggregated state of this sensitizer in the cells. AlPcS2 and AlPcS3 induced a lower degree of cell inactivation per fluorescing quantum and per quantum absorbed by monomeric species than did AlPcS2 and AlPcS1. AlPcS4 and AlPcS3 are therefore suggested to be in different intracellular locations than AlPcS2 and AlPcS1.
Berg
K
K
Bommer
J C
JC
Moan
J
J
D002478:Cells, Cultured; D007211:Indoles; D009942:Organometallic Compounds; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents; D013057:Spectrum Analysis; D013451:Sulfonic Acids
D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; D013451:Sulfonic Acids; C060570:aluminum phthalocyanine