Change in cytoplasmic free Ca2+ concentration in rabbit platelets contacting with albumin coated and uncoated polystyrene surfaces.
Direct measurement of cytoplasmic free Ca2+ concentration in rabbit platelets in contact with polystyrene latexes was examined by monitoring an intracellular trapped fluorescent indicator dye, Fura 2, as a quantitative evaluation of platelet activation on material surfaces. It was found that an increase in cytoplasmic free calcium levels in platelets in contacting latexes was strongly reduced by the adsorption of albumin on the latex surfaces. Further, such an elevation of cytoplasmic free calcium levels was found to require a calcium influx from an extracellular medium across the platelet-plasma membrane.
Institute of Biomedical Engineering, Tokyo Women's Medical College, Japan.
N
N
Yui
K
K
Kataoka
Y
Y
Sakurai
Y
Y
Fujishima
T
T
Aoki
A
A
Maruyama
K
K
Sanui
N
N
Ogata
D000818:Animals; D001572:Benzofurans; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D003593:Cytoplasm / Q000378:metabolism; D016257:Fura-2; D066298:In Vitro Techniques; D008297:Male; D010973:Platelet Adhesiveness; D011137:Polystyrenes; D011817:Rabbits; D012709:Serum Albumin; D013499:Surface Properties
D001572:Benzofurans; D001672:Biocompatible Materials; D011137:Polystyrenes; D012709:Serum Albumin; D002118:Calcium; D016257:Fura-2