Determination of the structure of a novel acidic oligosaccharide with blood-group activity isolated from bovine submaxillary-gland mucin.
In this study we investigated the structure of an acidic fucose-containing pentasaccharide released from bovine submaxillary-gland mucin by alkaline-borohydride treatment. The structure, determined by a combination of one-dimensional and two-dimensional 1H-n.m.r. spectroscopy at 270 MHz and methylation analysis involving g.l.c.-m.s., was as follows: Fuc alpha(1----2)Gal beta(1----4)GlcNAc beta(1----3)[NeuAc alpha(2----6)]GalNAcol This pentasaccharide is a novel structure and is the first report of a blood-group-H type 2 determinant on a submaxillary-gland mucin.
Department of Chemistry, University College, Galway, Ireland.
S M
SM
D'Arcy
C M
CM
Donoghue
C A
CA
Koeleman
D H
DH
Van den Eijnden
A V
AV
Savage
D000818:Animals; D001789:Blood Group Antigens; D002417:Cattle; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D009077:Mucins / Q000032:analysis*; D009844:Oligosaccharides / Q000302:isolation & purification* / Q000378:metabolism; D013329:Structure-Activity Relationship; D013363:Submandibular Gland / Q000032:analysis*
D001789:Blood Group Antigens; D009077:Mucins; D009844:Oligosaccharides