Determination of halofuginone in bovine plasma by competing-ion high performance liquid chromatography after solid phase extraction.
A high performance liquid chromatography (HPLC) method for the determination of the anticoccidial and antitheilerial drug halofuginone in bovine plasma was developed. Samples were diluted with acetic acid (10%, v/v) and cleaned up on a Bond Elut C8 column. The analyte was eluted from the extraction column and chromatographed by reversed-phase HPLC using decylamine as a competing-ion reagent. Detection was by UV at 243 nm. Recovery from plasma was 75%, and within-day and between-day coefficients of variation were 5.23 and 6.35% respectively. The specificity and sensitivity of this method (limit of detection in plasma, 1 ng/mL) were sufficiently high to enable us to characterize the time course of the drug in plasma after oral administration of therapeutic doses to cattle.
Department of Veterinary Pharmacology, University of Glasgow Veterinary School, UK.
L D
LD
Kinabo
Q A
QA
McKellar
M
M
Murray
D000284:Administration, Oral; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003049:Coccidiostats / Q000097:blood*; D007202:Indicators and Reagents; D008832:Microchemistry; D010880:Piperidines; D011799:Quinazolines / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification; D052999:Quinazolinones
D003049:Coccidiostats; D007202:Indicators and Reagents; D010880:Piperidines; D011799:Quinazolines; D052999:Quinazolinones; C010176:halofuginone