Evidence for regulation of endothelial plasminogen-activating system by polymorphonuclear leukocyte elastase.
Incubation of unstimulated polymorphonuclear leukocytes with cultured bovine pulmonary artery endothelial cells increased the activity of endothelial plasminogen activator. On the other hand, polymorphonuclear leukocytes stimulated by serum-opsonized zymosan decreased the plasminogen activator activity. A specific elastase inhibitor increased the enhancing effect of the unstimulated polymorphonuclear leukocytes and reversed the suppressing effect of the stimulated polymorphonuclear leukocytes. Catalytically active elastase suppressed the plasminogen activator activity and increased the activity of plasminogen activator inhibitor. In contrast, inactivated elastase enhanced the activity of plasminogen activator. Both, active and inactive forms of elastase bound to the endothelial cells. These findings suggest that elastase modulates the endothelial plasminogen-activating system, apparently by binding to the endothelial cells.
Wadsworth Center for Labs and Research, New York State Department of Health 12201-0509.
N
N
Gilboa
P H
PH
Neumann
J M
JM
Gutmann
P J
PJ
Del Vecchio
P W
PW
Gudewicz
D000818:Animals; D001769:Blood; D002417:Cattle; D002460:Cell Line; D003470:Culture Media; D004730:Endothelium, Vascular / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology*; D009895:Opsonin Proteins; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism*; D011651:Pulmonary Artery; D015054:Zymosan / Q000494:pharmacology
D003470:Culture Media; D009895:Opsonin Proteins; D015054:Zymosan; D010960:Plasminogen Activators; D010196:Pancreatic Elastase