Continuous-flow 13C-filtered 1H NMR spectroscopy of ethanol metabolism in rat liver perfusate.
Using a 188.5-microliters continuous-flow dual probe 1H[13C] spin-echo difference spectra of rat liver perfusate were acquired. The conversion of [1-13C]ethanol to [1-13C]-acetaldehyde was readily monitored as a function of time. In combination with 1-1 water nonexcitation and WALTZ 13C decoupling, this method proved to be superior in sensitivity and selectivity to direct 1H or 13C detection.
Institut für Organische Chemie der Universität Tübingen, Federal Republic of Germany.
K
K
Albert
J L
JL
Sudmeier
M S
MS
Anwer
W W
WW
Bachovchin
D000818:Animals; D002247:Carbon Isotopes; D004867:Equipment Design; D000431:Ethanol / Q000378:metabolism* / Q000493:pharmacokinetics; D006859:Hydrogen; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D002247:Carbon Isotopes; D000431:Ethanol; D006859:Hydrogen