Edema-producing proteins isolated from Trimeresurus mucrosquamatus snake venom.
Seven edema-producing fractions were isolated from Trimeresurus mucrosquamatus snake venom by CM-Sephadex C-50 chromatography and further purified by gel filtration on a Sephadex G-75 column. They were homogeneous as judged by electrophoresis on polyacrylamide gels. The mol. wts were estimated to be 26,900 (Fr. IV-2), 21,500 (Fr. X-2), 23,000 (Fr. XII-2), 21,800 (Fr. XIII-2), 24,600 (Fr. XIV-2), 80,000 (Fr. XVIII-1) and 22,500 (Fr. XXII-2). Fraction IV-2 had weak esterase activity, fractions X-2, XII-2, XIII-2 and XIV-2 possessed proteolytic activity toward casein and fibrinogen, while fractions XVIII-1 and XXII-2 possessed phospholipase A2 activity. Fractions with phospholipase A2 activity had greater edema-producing activity than those with protease and/or esterase activity. It is concluded that the edema caused by T. mucrosquamatus venom may be due to phospholipases A2, proteases and esterases.
Pharmacological Institute, College of Medicine, National Taiwan University, Taipei.
C M
CM
Teng
J P
JP
Wang
H C
HC
Peng
C
C
Ouyang
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D003435:Crotalid Venoms / Q000032:analysis* / Q000633:toxicity; D004487:Edema / Q000139:chemically induced*; D004589:Electrophoresis, Disc; D011506:Proteins / Q000302:isolation & purification* / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D003435:Crotalid Venoms; D011506:Proteins; C029748:Trimeresurus venoms