Comparison of an immunocytochemical assay for progesterone receptor with a biochemical method of measurement and immunocytochemical examination of the relationship between progesterone and estrogen receptors.
Using a rat monoclonal antibody raised against human progesterone receptor (PR) we have developed an immunocytochemical technique to detect PR in human normal and malignant breast tissue and have compared the distribution of this with that obtained by the conventional dextran-coated charcoal steroid-binding assay. Immunoreactive PR was detected exclusively in the nuclei of epithelial cells in 29/51 (56.9%) of breast cancers studied. There was an excellent correlation between the immunocytochemical and dextran-coated charcoal techniques, with concordance in 43/51 (84.3%) cases [regression coefficient (Spearman) = 0.78; P less than 0.001]. The relationship between PR and estrogen receptor (ER) was also examined immunocytochemically using a monoclonal antiserum to ER. Twenty-eight out of 51 (54.9%) tumors were positive for both receptors and 13/51 (25.5%) negative for both. ER-positive, PR-negative tumors were found in 9/51 (17.6%) cases whereas only one case (2%) was PR-positive, ER-negative.
Ludwig Institute for Cancer Research, St. George's Hospital Medical School, London, United Kingdom.
U
U
Berger
P
P
Wilson
S
S
Thethi
R A
RA
McClelland
G L
GL
Greene
R C
RC
Coombes
D000911:Antibodies, Monoclonal; D001940:Breast / Q000032:analysis / Q000166:cytology; D001943:Breast Neoplasms / Q000032:analysis* / Q000473:pathology; D003600:Cytosol / Q000032:analysis; D005260:Female; D005348:Fibrocystic Breast Disease / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D011397:Promegestone / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011397:Promegestone