Volume changes in the binding of lanthanides to peptide analogues of loop II of calmodulin.
The solution expansion accompanying coordination of lanthanide ions to synthetic peptide analogues of a metal-binding loop in calmodulin was determined by a density method. This study was designed to further test the hypothesis that the nonlinear expansions observed upon sequential addition of Ca2+ to intracellular calcium-binding proteins reflect principally upon the coordination event at specific binding sequences. Three peptides of 13 residues each were synthesized as analogues of binding loop II in mammalian calmodulin: Peptide I was the native analogue; peptide II contained an aspartyl in place of an asparaginyl residue at position 5 from the N-terminus; for peptide III, the aspartyl residue in position 3 of the native analogue was interchanged with the asparaginyl residue in position 5. Thus, the number of charged-oxygen donor atoms for coordination was the same in I and in III, but the latter peptide could permit two pairs of acidic groups to converge toward the metal ion as in some loops of these proteins. The observed expansions with different lanthanide ions to the same peptide varied appreciably, suggesting dissimilar structures [Gariépy et al. (1983) Biochemistry 22, 1765-1772]; coordination to the simpler tetracarboxylate sequestrants, on the other hand, generated an expansion profile approximately as expected from the properties of the lanthanide series. The largest expansions were generated with peptide II (having the additional acidic group) for all lanthanides tested.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Biochemistry, School of Medicine, University of Virginia, Charlottesville 22908.
D W
DW
Kupke
J W
JW
Fox
D001665:Binding Sites; D002118:Calcium; D002147:Calmodulin; D008674:Metals, Rare Earth; D010455:Peptides / Q000138:chemical synthesis; D011487:Protein Conformation; D014336:Troponin; D019209:Troponin C
D002147:Calmodulin; D008674:Metals, Rare Earth; D010455:Peptides; D014336:Troponin; D019209:Troponin C; D002118:Calcium