Evaluation of HLA expression on gametogenic cells isolated from human testis.
Human germinal cells were isolated using a combined mechanical method and enzymatic digestion of testicular tissue. The attempt to fractionate the testicular cells by centrifugation in the continuous and discontinuous Percoll gradient was undertaken. The homogeneous (100%) fraction of late spermatids and enriched fractions of spermatocytes (up to 60%) and round spermatids (approx. 66%) were obtained. Cell-binding radioimmunoassay (CB-RIA) was used to evaluate the HLA expression both on heterogeneous suspensions and enriched fractions of testicular, germ cells using mouse monoclonal antibodies directed to Class I (HLA, -A, -B, -C) and Class II (HLA, -DR) determinants of MHC (main histocompatibility complex). Consistently negative results were obtained as well for heterogenous populations of germinal cells as their fractions in respect to both Class I and Class II antigenic determinants of HLA system.
Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
I
I
Kowalik
M
M
Kurpisz
A
A
Jakubowiak
A
A
Janecki
A
A
Lukaszyk
G
G
Szymczynski
D000328:Adult; D000911:Antibodies, Monoclonal; D002499:Centrifugation, Density Gradient; D005854:Germ Cells / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D008297:Male; D011863:Radioimmunoassay; D013087:Spermatids / Q000032:analysis; D013090:Spermatocytes / Q000032:analysis; D013737:Testis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II