Differential cyclophosphamide sensitivity of suppressor function in Xenopus, the clawed toad.
Thymocyte and Splenocyte cultures from in vivo immunised Xenopus were assayed to test their suppressive capacity. Immunisation with TNP-Polyvinylpyrrolidone induced suppression. Suppression induced by the haptenated antigens, TNP-Red blood cells, TNP-Lipopolysaccharide, and TNP-Ficoll affected the anti-TNP antibody response of splenocytes from TNP-PVP immunised animals. Pretreatment with cyclophosphamide revealed both a sensitive and insensitive suppression capacity in Xenopus laevis.
Department of Human Morphology, University of Nottingham, Queen's Medical Centre, UK.
R H
RH
Clothier
Z
Z
Last
J D
JD
Somauroo
L N
LN
Ruben
M
M
Balls
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D007108:Immune Tolerance / Q000187:drug effects*; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011205:Povidone / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D014301:Trinitrobenzenes / Q000276:immunology; D014981:Xenopus / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014301:Trinitrobenzenes; D003520:Cyclophosphamide; D011205:Povidone