Microplate measurement of urinary albumin and creatinine.
We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly simplifying calculations and improving sensitivity and accuracy. Measurement of creatinine in urine and plasma samples with a commercially available enzymatic kit modified for analysis by use of an inexpensive microplate reader produced values comparable in precision and accuracy to those obtained by an automated kinetic Jaffé method.
Department of Internal Medicine, University of Cincinnati, OH 45267-0585.
R A
RA
Magnotti
G W
GW
Stephens
R K
RK
Rogers
A J
AJ
Pesce
D000328:Adult; D000419:Albuminuria / Q000175:diagnosis / Q000652:urine*; D002624:Chemistry, Clinical / Q000592:standards; D003404:Creatinine / Q000592:standards / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic; D003404:Creatinine