Glycine-extended anglerfish peptide YG (aPY) a neuropeptide Y (NPY) homologue may be a precursor of a biologically active peptide.
The 37 residue peptide YG (aPY), isolated from anglerfish endocrine pancreas, bears distinct sequence homology to the pancreatic polypeptide family of hormones. However, instead of a carboxyl-terminal tyrosine-amide, aPY has a free carboxyl-terminus ending with glycine. Towards studying the structure-activity relationship of this hormone, we have synthesized aPY by solid phase methodology using Boc-amino acid derivatives and phenylacetamidomethyl resin. The crude peptide was purified to homogeneity in 20% yield by reversed phase chromatography. The purified peptide had the expected amino acid composition and sequence, and was found to be identical with the natural aPY by analytical HPLC and peptide mapping of proteolytic digests. Neither the snythetic nor the natural aPY exhibited the characteristic vasoconstrictor activity of the related pancreatic polypeptide family of hormones. However, [Des37-Gly]-aPY, isolated from the anglerfish pancreas, caused vasoconstriction in rats. Based on these results and by analogy to the glycine-extended gastrin peptides, it may be suggested that aPY is a precursor of a biologically active peptide, namely [Des37-Gly]-aPY-amide.
Department of Surgery, University of Cincinnati Medical Center, OH 45267.
A
A
Balasubramaniam
P C
PC
Andrews
V
V
Renugopalakrishnan
D F
DF
Rigel
D000595:Amino Acid Sequence; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D005399:Fishes; D006339:Heart Rate / Q000187:drug effects; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009478:Neuropeptide Y; D009479:Neuropeptides / Q000138:chemical synthesis* / Q000494:pharmacology; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014357:Trypsin
D007202:Indicators and Reagents; D009478:Neuropeptide Y; D009479:Neuropeptides; D010446:Peptide Fragments; C061057:glycine-extended anglerfish peptide YG; D014357:Trypsin