Quantitative analysis of wide-field specular microscopy. II. Precision of sampling from the central corneal endothelium.
The precision of the measurement of mean endothelial cell area obtained by sampling with small-field and wide-field specular microscopy from the central 4 mm of human corneal endothelium was studied by comparing endothelial cell parameters from individual specular micrographs in vivo to the results obtained by montaging the micrographs from the entire central 4 mm of the same corneas. The small samples were at least 10% from the true mean cell size of all cells of the central 4 mm in any endothelium other than that with the most homogeneous pattern. A new algorithm for sampling with these two specular microscopes will need to be derived to permit a more precise measure of the mean area of endothelial cells in the central 4 mm of the human corneal endothelium.
Department of Surgery, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
L W
LW
Hirst
K
K
Yamauchi
C
C
Enger
W
W
Vogelpohl
V
V
Whittington
D002452:Cell Count; D004728:Endothelium, Corneal / Q000166:cytology*; D006801:Humans; D008853:Microscopy; D010781:Photography; D011897:Random Allocation; D013048:Specimen Handling