Murine monoclonal antibody adsorbed onto vinylidene fluoride floccules used to eliminate antibody interference in "sandwich"-type immunoassays.
We have previously reported that human anti-mouse IgG antibody (HAMA) can cause false-positive and false-negative results in "sandwich"-type monoclonal antibody (MAb) assays. To eliminate HAMA interference in "sandwich"-type MAb assays, we investigated the use of MAb on solid-phase, vinylidene fluoride floccules, which we have previously used as a solid-phase second antibody for RIA. The simple procedure effectively removes greater than 95% of HAMA from the most positive serum we have obtained from patients hyperimmunized to murine MAb, and it allows for accurate quantification of carcinoembryonic antigen. The solid-phase complex, added to blood, effectively removes HAMA and (or) "HAMA-type" heterophilic antibody from the sera or plasma.
Newman
E S
ES
Immunomedics Inc., Newark, NJ 07103.
Moskie
L A
LA
Duggal
R N
RN
Goldenberg
D M
DM
Hansen
H J
HJ
D000327:Adsorption; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D014753:Vinyl Compounds
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D014753:Vinyl Compounds; C007384:1,1-difluoroethylene