cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.
The Ku antigen is a DNA-associated nuclear protein recognized by sera from patients with autoimmune diseases. It consists of two polypeptides of 86 and 70 kDa. cDNA clones encoding the 86-kDa subunit of the Ku antigen were isolated by probing lambda gt11 recombinant cDNA expression libraries with a monoclonal antibody specific for this protein. The amino acid sequence deduced from the cDNA comprises 732 amino acids and corresponds to a protein with molecular weight of 81.914. Nineteen residues at the NH2 terminus determined by protein sequencing corresponded to the sequence deduced from the cDNA. The predicted amino acid sequence contains a region with repeating leucine residues similar to the "leucine zipper" structure observed in the c-myc, v-myc, and c-fos oncogene products. The largest cDNA hybridized to 2.7- and 3.4-kilobase poly(A)+ mRNAs from HeLa cells. The cDNA clones expressed fusion proteins immunoreactive with the monoclonal antibody and sera from patients with autoimmune diseases.
Yaneva
M
M
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030.
Wen
J
J
Ayala
A
A
Cook
R
R
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005333:Fetus; D006801:Humans; D000072200:Ku Autoantigen; D008099:Liver / Q000032:analysis; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D011487:Protein Conformation; D015183:Restriction Mapping
D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D000954:Antigens, Surface; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D004247:DNA; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen