Molecular evidence of Y-autosomal translocations in owl monkeys.
Probe pDP1007, which contains highly conserved DNA sequences from the sex-determining region of the human Y chromosome, cross-hybridized with owl monkey EcoRI restriction fragments of 1.8 kb and 6.6 kb. Southern transfer analysis of owl monkey (karyotype VI)--rodent somatic cell hybrids localized the 1.8-kb fragment on the owl monkey X chromosome and the 6.6-kb fragment, which is male specific, on chromosome 14/Y. Regional in situ chromosome mapping of pDP1007 revealed specific sites of hybridization: the distal short arm of the X chromosome of karyotypes IV, VI, and VII; the small metacentric Y of karyotype IV; the C-band positive region on the short arm of chromosome 17/Y (karyotype VII); and the C-band positive region on the long arm of chromosome 14/Y (karyotype VI). These molecular findings reinforce cytological evidence that Y-chromosomal material has been transferred to autosomes 14 and 17 in owl monkeys of karyotypes VI and VII, respectively, in which there are no independently segregating Y chromosomes.
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New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772.
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D000818:Animals; D001029:Aotus trivirgatus / Q000235:genetics*; D015139:Blotting, Southern; D002427:Cebidae / Q000235:genetics*; D002874:Chromosome Mapping; D015342:DNA Probes; D005260:Female; D006801:Humans; D008297:Male; D012732:Sex Determination Analysis; D014178:Translocation, Genetic; D014960:X Chromosome; D014998:Y Chromosome
D015342:DNA Probes