Remineralization of bovine dentine in vitro. The influence of the F content in solution on mineral distribution.
In this paper remineralization of bovine dentine is reported. After demineralization of the dentine in an acidic gel system creating lesions of about 180 microns depth, the tissue was remineralized in a 1.5 mM Ca and 0.9 mM phosphate containing solution at pH 7 and 37 degrees C for 8 or 21 days. The F content in the remineralization solution was 0.02, 2, or 10 ppm as NaF. Samples were analyzed by means of microradiography and scanning electron microscopy. The results show that remineralization (without F added in solution) causes a decrease in mineral loss and in lesion depth. With 2 or 10 ppm in solution, however, a substantial mineral accumulation in the lesion, but particularly on the original dentine surface, was observed. During 3 weeks of remineralization, mineral accumulations of 67 and 70 vol% of mineral for 2 and 10 ppm F in solution, respectively, were found near the original outer surface, with the sound dentine mineral value being 48 vol%. The combined microradiography and scanning electron microscopy data show that the mineral accumulated is for a major part deposited on the dentine tissue and partly inside. The latter deposition occurred both inside the tubules as well as in the intertubular areas. Inside the tubules dense precipitates were observed. Because several differences exist between bovine and human dentine, an extrapolation to the human in vivo situation is speculative. The results indicate that the presence of fluoride in the parts per million range is important for dentine remineralization efficacy and that the outer surface area in dentine can be 'overremineralized'.
Dental School, Laboratory for Materia Technica, Groningen, The Netherlands.
J
J
Arends
J
J
Christoffersen
J
J
Ruben
W L
WL
Jongebloed
D000818:Animals; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects / Q000378:metabolism; D003804:Dentin / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005459:Fluorides / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D008852:Microradiography; D008855:Microscopy, Electron, Scanning; D014089:Tooth Remineralization / Q000379:methods*
D005459:Fluorides