[Optimization of the purification of human plasma fibronectin by double affinity chromatography using gelatin and heparin Trisacryl LS].
The authors described an optimized method of preparation to obtain pure human plasma fibronectin. The new performances related to the chromatographic and concentration steps, where the saving of time is about 40% and the lyophilization step where the saving of energy is also about 40%. The final product is without any less of either physical and biological activities. The size of the columns, the volumes of the chromatographic supports (gelatin and heparin-Trisacryl LS) and the quantity of the treated plasma are very much important and also the quantities of the final product are very increased.
Allary
M
M
Centre de Transfusion sanguine des Armées Jean-Julliard, Clamart.
Amoignon
T
T
Lafargue
O
O
Sene
C
C
Boschetti
E
E
Saint-Blancard
J
J
D000180:Acrylic Resins; D002846:Chromatography, Affinity / Q000379:methods*; D005353:Fibronectins / Q000097:blood* / Q000302:isolation & purification; D005612:Freeze Drying; D005780:Gelatin; D006493:Heparin; D006801:Humans
D000180:Acrylic Resins; D005353:Fibronectins; D005780:Gelatin; D006493:Heparin
Optimisation de la purification de la fibronectine plasmatique humaine par double chromatographie d'affinité sur gélatine et héparine Trisacryl LS.