Further characterization of serum alkaline phosphatase from male and female beagle dogs.
Alkaline phosphatase (AP) from the sera of both male and female beagle dogs was partially purified and then analyzed for the presence of AP isoenzymes having intestinal or osseous characteristics as detected by bromotetramisole inhibition or wheat germ lectin agarose electrophoresis, respectively. The sera from both sexes were similar in regard to the presence of AP isoenzymes with intestinal (16 vs. 20%) or osseous (19 vs. 23%) characteristics, but serum AP from the male had a greater sialic acid content and only the male serum contained a detectable constitutive acidic (pI = 3.4) AP isoenzyme. This was similar to a serum AP isoenzyme previously found elevated in the sera of dogs afflicted with hyperadrenocorticalism or of dogs treated with certain corticosteroids.
Drug Safety Evaluation Department, Pfizer Central Research, Groton, Conn.
D E
DE
Amacher
C V
CV
Higgins
S J
SJ
Schomaker
R J
RJ
Clay
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood* / Q000302:isolation & purification; D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004285:Dogs / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007527:Isoenzymes / Q000032:analysis / Q000097:blood; D008297:Male; D008970:Molecular Weight; D012685:Sepharose / Q000031:analogs & derivatives; D012794:Sialic Acids / Q000032:analysis; D013773:Tetramisole / Q000031:analogs & derivatives / Q000494:pharmacology; D014462:Ultrafiltration
D007527:Isoenzymes; D012794:Sialic Acids; C028326:concanavalin A-sepharose; C006226:4-bromotetramisole; D012685:Sepharose; D013773:Tetramisole; D000469:Alkaline Phosphatase