Changes in levels of osteonectin in bovine dentine during tooth development.
Bovine incisors were classified into three developmental stages and non-collagenous proteins extracted from them. Sodium dodecyl sulphate gel electrophoresis of the extracts showed a reduction in osteonectin with the various stages. The reduction was confirmed by enzyme immunoassay using antiserum against bone osteonectin. This change is in contrast to dentine phosphoprotein, indicating functional differences between these two proteins.
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
R
R
Fujisawa
Y
Y
Kuboki
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002417:Cattle; D003804:Dentin / Q000032:analysis* / Q000254:growth & development; D004586:Electrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D007180:Incisor / Q000032:analysis / Q000254:growth & development*; D015676:Osteonectin
D002352:Carrier Proteins; D015676:Osteonectin