A simple method for the Schwann cell preparation from newborn rat sciatic nerves.
We have developed a simple and relatively rapid method for obtaining a sufficient number of Schwann cells with a favorable purification ratio from newborn rat sciatic nerves. Perineurium-free nerves were torn into small fascicles of approximately 150-200 microns in diameter and explanted twice on type I collagen gel every 2 days of the culture period in order to reduce the number of contaminant fibroblasts. The last explanted tissues were fed with Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum and 100 micrograms/ml bovine pituitary extract for 10 days. More than 10 X 10(4) Schwann cells (greater than 95% purity) were obtained from newborn rat sciatic nerves.
Oda
Y
Y
Department of Pathology, School of Medicine, Kanazawa University, Japan.
Okada
Y
Y
Katsuda
S
S
Ikeda
K
K
Nakanishi
I
I
D000818:Animals; D000831:Animals, Newborn; D046508:Culture Techniques / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism; D012584:Sciatic Nerve / Q000166:cytology*
D009418:S100 Proteins