[Cytogenetic effect of the radiosensitizing action of 5-bromo-2'-deoxyuridine on eukaryotic cells at various periods of the DNA synthesis phase].
The effect of 5-bromo-2'-deoxyuridine (BUDR) radiosensitizing action on meristematic cells of wheat seedlings and human peripheral blood lymphocytes synchronized with 5-fluoro-2'-deoxyuridine at the G1-S boundary was revealed. This effect was only found in the beginning of the S phase. We show that it was conditioned by incorporation of BUDR into special early replicating DNA sequences which are specific for chromosomal mutagenesis. Using the molecular biology methods non-random distribution of 3H-BUDR in the early replicating wheat DNA sequences with different degree of repeating was found.
Kh A
KhA
Khakimov
Ts A
TsA
Chikhladze
G A
GA
Khudoliĭ
A I
AI
Akuf'ev
D001345:Autoradiography; D001973:Bromodeoxyuridine / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000187:drug effects* / Q000528:radiation effects; D004261:DNA Replication / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D007399:Interphase; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D009154:Mutation; D009695:Nucleic Acid Renaturation; D011838:Radiation-Sensitizing Agents / Q000633:toxicity*; D014908:Triticum / Q000235:genetics
D011838:Radiation-Sensitizing Agents; D001973:Bromodeoxyuridine
Tsitogeneticheskiĭ éffekt radiosensibiliziruiushchego deĭstviia 5-brom-2'-dezoksiuridina na kletki éukariot v otdel'nykh otrezkakh fazy sinteza DNK.