A physical map of the genome of Ureaplasma urealyticum 960T with ribosomal RNA loci.
A physical map is presented for the 900 kilobase pair genome of Ureaplasma urealyticum 960T, locating 29 sites for 6 restriction endonucleases. The large restriction fragments were separated and sized by pulsed-field agarose gel electrophoresis (PFGE). Their locations on the map were determined by probing Southern blots of digests with individual fragments isolated from other digests and by correlating the products of double digestions and partial digestions. An end-labelling technique was used to detect small fragments not readily observed by PFGE. Two loci for rRNA genes have been determined by probing with cloned DNA.
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
B G
BG
Cocks
L E
LE
Pyle
L R
LR
Finch
D001482:Base Composition; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D012335:RNA, Ribosomal / Q000235:genetics*; D015183:Restriction Mapping; D014509:Ureaplasma / Q000235:genetics*
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal