Role of GSSG-reductase and a thiol oxidant diethylmaleate (DEM) in skin tumorigenesis induced by jute batching oil.
Single topical application of jute batching oil (JBO-P) elevated the status of enzyme GSSG-reductase in mouse skin and multiple applications produced a persistent increase in the enzyme levels. Also an increase in NADPH-dependent GSSG-reductase activity was registered after single topical application of known carcinogenic polyaromatic hydrocarbons (PAHs), e.g. benzo(a)pyrene (BaP), 7,12-dimethyl-benzanthracene (DMBA) and 3-methylcholanthrene (3-MC). This suggests that the change in GSSG-reductase activity induced by JBO-P is intrinsic to its tumorigenic activity rather than the toxic effect of the oil. Pretreatment of mouse skin with diethylmaleate (DEM), an SH-inactivating agent, increases the latent period of JBO-P induced tumorigenesis. No tumour was recorded in animals belonging to Group IV (DEM + JBO) while in animals belonging to Group II (JBO-P alone) 100% tumorigenesis was recorded during the period of study (i.e. up to 20 wk).
Environmental Carcinogenesis Laboratory, Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow, India.
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Antony
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Kumar
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Mehrotra
D000818:Animals; D002273:Carcinogens; D005260:Female; D005980:Glutathione Reductase / Q000032:analysis / Q000502:physiology*; D008298:Maleates / Q000494:pharmacology*; D051379:Mice; D008899:Mineral Oil / Q000633:toxicity*; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000201:enzymology
D002273:Carcinogens; D008298:Maleates; C051384:jute batching oil; D008899:Mineral Oil; D005980:Glutathione Reductase; C014476:diethyl maleate