Technique for cellular fluorescence distribution analysis.
The usefulness of multidimensional slit-scan flow cytometry in whole cell measurements is dependent on extracting relevant features from the cellular fluorescence distributions (slit-scan contours). In addition, the extraction of these features must be rapid to allow for real-time data processing during acquisition. This paper describes two algorithms that have been used successfully to count the numbers of local maxima (peaks) and to find nuclear boundaries in a cellular fluorescence distribution. These routines are efficient, use only simple integer arithmetic, and have been implemented on several different microprocessors.
Robinson
R D
RD
Department of Pathology, University of Rochester Medical Center, New York 14642.
Reeder
J E
JE
Wheeless
L L
LL
D000465:Algorithms; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D006801:Humans