The mouse thymidylate synthase promoter: essential elements are in close proximity to the transcriptional initiation sites.
The promoter region of the mouse thymidylate synthase gene was analyzed by deletion and site-directed mutagenesis. Elimination of an upstream Sp1 element reduced expression threefold, whereas elimination of an adenovirus upstream stimulatory factor element had little effect. All of the upstream elements that are essential for promoter activity are located within 22 nucleotides of the first transcriptional initiation site.
Department of Biochemistry, Ohio State University, Columbus 43210.
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Deng
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Li
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Jolliff
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Johnson
D000818:Animals; D001483:Base Sequence; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics; D005809:Genes, Regulator; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D013940:Thymidylate Synthase / Q000235:genetics*; D014158:Transcription, Genetic
D004247:DNA; D013940:Thymidylate Synthase