[Use of FMC macromolecular medium for determination of the erythrocyte ABO group using microtiter plates].
Several blood group determination techniques have been developed in recent years which provide easy handling, fine reproducibility and similar or even higher sensitivity than the procedures commonly employed, aimed to be used in the automation of blood bank work. A microtitration plate technique for ABO determination on red cells is presented in this report. A macromolecular medium, comprised of a mixture of the polymers Ficoll 400 and carboxymethyl cellulose (FMC) is used ad coadjuvant for the reaction. This medium increases by 15- the score units and by 4-fold (or more) the intensity of haemagglutination (titration) in typing the ABO antigenicity of red cell A, B, O and AB groups.
J
J
Mauri
M G
MG
Ercilla
A
A
Gelabert
R
R
Castillo
D000017:ABO Blood-Group System / Q000235:genetics; D001788:Blood Grouping and Crossmatching / Q000295:instrumentation / Q000379:methods*; D002266:Carboxymethylcellulose Sodium; D004912:Erythrocytes / Q000276:immunology*; D005362:Ficoll; D006386:Hemagglutination Tests / Q000295:instrumentation; D006801:Humans; D012996:Solutions
D000017:ABO Blood-Group System; D012996:Solutions; D005362:Ficoll; D002266:Carboxymethylcellulose Sodium
Empleo del medio macromolecular FMC para la determinación del grupo eritrocitario ABO empleando placas de microtitulación.