"In vivo" (35S)methionine interaction with rat liver tRNA.
As part of a study to characterize the methionine role in tumorigenesis, we report that methionine sulfur interacts with rat liver tRNA "in vivo" (35S) radioactivity remained associated to the nucleic acid after a number of treatments, including tRNA deacylation. Similar data were obtained after administration of (methyl-3H) methionine, while no comparable tRNA labelling was detected when the aminoacid labelled in the aliphatic chain was given. Hplc analysis of (35S) tRNA enzymic hydrolysate showed two unidentified UV-absorbing radioactive peaks. NMR spectra of these two peaks did not reveal any thiomethyl group.
Dipartimento di Biochimica e Biologia Moleculare, Facoltá di Scienze, Universitá di Bari, Italy.
D
D
Kanduc
M R
MR
Rossiello
T
T
Ranieri
E
E
Quagliariello
D000818:Animals; D004247:DNA / Q000378:metabolism; D008099:Liver / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013455:Sulfur
D013455:Sulfur; D004247:DNA; D012343:RNA, Transfer; D008715:Methionine