Identification and determination of 1 alpha,25-dihydroxyvitamin D3 in rat skin by high-performance liquid chromatography and radioreceptor assay.
Endogenous 1 alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in normal rat skin was identified by using thermal isomerization to convert the metabolite into its pre-isomer, high-performance liquid chromatography (HPLC) and displacement potency with a chick intestinal cytosol receptor. When the metabolite in normal rat skin was determined by a radioreceptor assay after purification by Sep-Pak silica cartridge column chromatography and HPLC, the concentration was 71.0 +/- 6.6 pg/g of wet tissue (mean +/- S.D.). It is also shown that [3H]-1,25-(OH)2D3 and [3H]-25-hydroxyvitamin D3 administered intravenously in the mouse are located in the skin. These results suggest that the metabolite may play an important role in the skin.
Department of Hygienic Sciences, Kobe Women's College of Pharmacy, Japan.
T
T
Okano
N
N
Tsugawa
T
T
Kobayashi
D000818:Animals; D002117:Calcitriol / Q000032:analysis*; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D051379:Mice; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000032:analysis*
D002117:Calcitriol