Strain variation in Campylobacter pylori detected by numerical analysis of one-dimensional electrophoretic protein patterns.
A total of 21 clinical isolates of Campylobacter pylori from Peru and the United Kingdom and two reference strains (from Australia), including the type strain (NCTC 11637T), were characterized by high resolution one-dimensional SDS-polyacrylamide gel electrophoresis of cellular proteins. The protein patterns contained more than 40 discrete bands and the approximate molecular weights of the major bands were 22, 27, 46, 57, 60, 65 and 93 kD. The total patterns were used as the basis of numerical analysis. Most strains were clustered in four phenons at 91% similarity with the exception of six ungrouped strains. Overall similarity was high with all strains linked in the phenogram at greater than or equal to 81%. Variation among strains was attributable principally to qualitative and quantitative band differences in the 47 to 56 kD (hypervariable) region of the C. pylori protein profile. From the analysis, ten different electropherotypes (EP-types) were identified. We demonstrated that differences were detectable among isolates from widely separated geographical locations as well as from the same location, although multiple isolates from two Peruvian patients had the same electropherotype. Our results indicate that determination of protein profiles provides the basis of a reproducible method for characterization of C. pylori isolates.
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
R J
RJ
Owen
M
M
Costas
D D
DD
Morgan
S L
SL
On
L R
LR
Hill
A D
AD
Pearson
D R
DR
Morgan
D001426:Bacterial Proteins / Q000032:analysis*; D002167:Campylobacter / Q000032:analysis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006801:Humans; D009716:Numerical Analysis, Computer-Assisted; D015203:Reproducibility of Results; D013045:Species Specificity
D001426:Bacterial Proteins