Dexamethasone induces an increased biosynthesis of chromogranin A in rat pituitary gland.
The influence of dexamethasone on the regulation of the biosynthesis of chromogranins A and B, two proteins originally described in adrenal chromaffin granules, was investigated in the rat pituitary gland. Dexamethasone increased chromogranin A levels and its corresponding mRNA in a dose-dependent manner. Induction of chromogranin A occurred with a time-lag of 24 h. As shown by immunohistochemistry, chromogranin A was present in control and dexamethasone-treated rats in gonadotrophic cells only. Chromogranin B, secretogranin II and the respective pituitary hormones co-stored with chromogranin A were not changed by dexamethasone. Thus dexamethasone selectively increased chromogranin A levels in gonadotrophic cells.
Department of Pharmacology, University of Innsbruck, Austria.
R
R
Fischer-Colbrie
T
T
Wohlfarter
K W
KW
Schmid
M
M
Grino
H
H
Winkler
D000818:Animals; D053379:Chromogranin A; D002864:Chromogranins / Q000096:biosynthesis* / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010902:Pituitary Gland / Q000187:drug effects*; D010908:Pituitary Hormones, Anterior / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013997:Time Factors
D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D010908:Pituitary Hormones, Anterior; D011506:Proteins; D012333:RNA, Messenger; C506153:chromogranin A, rat; D003907:Dexamethasone