Histamine methyltransferase in dispersed cells from rabbit fundic mucosa.
The cellular distribution of histamine N-methyltransferase was studied in rabbit gastric mucosa. The fundic mucosa was dispersed by collagenase treatment in Hanks' or calcium-free medium. In calcium-free medium, the number of dispersed cells/g wet tissue, as well as their viability was increased; histamine N-methyltransferase recovery was up to three-fold larger than in cells prepared in Hanks' medium. Furthermore, the calcium-free medium led to a greater acid secretory response, whereas the cellular pepsinogen content tended to be lower. Histamine N-methyltransferase activity was found in all cell fractions but was higher in the larger cell types. The enzyme activity showed only a partial correlation with either oxyntic or chief cells. These results indicate that the use of calcium-free medium to disperse and isolate rabbit mucosal cells improves cell quality. Histamine N-methyltransferase in the rabbit fundic mucosa, is found in more than one cell type, primarily the oxyntic and chief cells.
Loiselle
J
J
Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Wollin
A
A
D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D005748:Gastric Fundus / Q000166:cytology / Q000201:enzymology; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology*; D006637:Histamine N-Methyltransferase / Q000032:analysis*; D008780:Methyltransferases / Q000032:analysis*; D010295:Parietal Cells, Gastric / Q000201:enzymology; D011817:Rabbits
D000632:Aminopyrine; D008780:Methyltransferases; D006637:Histamine N-Methyltransferase