Ultraviolet B light inactivates bone marrow T lymphocytes but spares hematopoietic precursor cells.
Bone marrow cells from ten normal donors were exposed to ultraviolet (UV)C or UVB light for total exposures of 0.1 to 100 mJ/cm2, and assayed for granulocyte-macrophage colony-forming units (CFU-GM), erythroid burst-forming units (BFU-E), and phytohemagglutinin (PHA)-stimulated proliferative responses. After exposure to UVC CFU-GM, BFU-E and PHA responses showed a UV dose-dependent sharp decrease to levels less than 1% of controls with 0.5, 2.0, and 10 mJ/cm2, respectively. With UVB, PHA responses were most sensitive, declining to less than 1% at 5 mJ/cm2. BFU-E decreased to less than 1% of control with 15 mJ/cm2 UVB. CFU-GM, at UVB doses of 0.1 to 2.0 mJ/cm2, increased to 125% to 130% of control and decreased to less than 1% only at exposures greater than 20 mJ/cm2. Thus, these studies show that UVB, but not UVC light, can be used to inactivate bone marrow T lymphocytes selectively while sparing hematopoietic precursor cells. The data suggest that UVB irradiation can be used for T-lymphocyte purging for allogeneic marrow transplantation.
Deeg
H J
HJ
Bone Marrow Transplantation Program, Georgetown University, Washington, DC.
Bazar
L
L
Sigaroudinia
M
M
Cottler-Fox
M
M
D001853:Bone Marrow / Q000528:radiation effects*; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D006410:Hematopoiesis / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D006801:Humans; D008213:Lymphocyte Activation / Q000528:radiation effects*; D008212:Lymphocyte Depletion; D013601:T-Lymphocytes / Q000528:radiation effects*; D014466:Ultraviolet Rays