Amplification of N-myc and ornithine decarboxylase genes in human neuroblastoma and hydroxyurea-resistant hamster cell lines.
The genes for the M2 subunit of ribonucleotide reductase (RRM2), ornithine decarboxylase (ODC1), and 55,000-Daltons protein (P5), are amplified in hydroxyurea-resistant hamster and human cell lines. These genomic sequences have been mapped to hamster chromosome 7 and to human chromosome 2p24-25 near the cytogenetic location of the N-myc gene. We now report that genomic sequences homologous to N-myc are amplified in hydroxyurea-resistant hamster lung cell line, 600H, and the N-myc gene segregates with hamster chromosome 7 in mouse-hamster somatic cell hybrids. The conserved linkage group consisting of the RRM2, ODC1, P5, and N-myc in the hamster and human genomes prompted our investigation of human neuroblastomas. We report here that genomic DNA from 1 of 6 primary neuroblastoma tumors containing amplified N-myc also contains amplified sequences homologous to a hamster ODC cDNA.
Department of Microbiology, University of Toronto, Toronto, M5S 1A8, Canada.
P N
PN
Tonin
H
H
Yeger
R L
RL
Stallings
P R
PR
Srinivasan
W H
WH
Lewis
D000293:Adolescent; D000818:Animals; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D004351:Drug Resistance; D005784:Gene Amplification; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D007223:Infant; D009447:Neuroblastoma / Q000235:genetics*; D009955:Ornithine Decarboxylase / Q000235:genetics*; D011519:Proto-Oncogenes; D014407:Tumor Cells, Cultured
D004247:DNA; D009955:Ornithine Decarboxylase; D006918:Hydroxyurea