The effect of adenine nucleotides on the rate and fate of glutamine utilization by incubated mitochondria isolated from rat mesenteric lymph nodes.
The rate of glutamine utilization by isolated lymphocyte mitochondria is 21.4 nmol/min per mg protein, of which 72% is converted to glutamate. Addition of ATP, ADP or AMP increased the rate of glutamine utilization by 60%. Evidence is presented that this is due to a stimulation of oxoglutarate dehydrogenase activity by ADP: this may account for the stimulation of glutamine utilization by concanavalin A in lymphocyte.
Curi
R
R
Department of Biochemistry, Oxford University, United Kingdom.
Newsholme
E A
EA
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D005972:Glutaminase / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D066298:In Vitro Techniques; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000227:Adenine Nucleotides; D005973:Glutamine; D007655:Ketoglutarate Dehydrogenase Complex; D005972:Glutaminase