Development and testing of a synthetic oligonucleotide probe for the detection of pathogenic Yersinia strains.
A 24-base oligonucleotide probe specific for a region of the Yersinia enterocolitica virulence plasmid (pYV) associated with HEp-2 cell cytotoxicity and the Sereny reaction was constructed by using sequences flanking critical TnphoA insertions in a subcloned fragment of pYV. This probe, highly specific and sensitive for virulent yersiniae, detected pathogenic Y. enterocolitica isolates in artificially inoculated foods.
Department of Medicine, University of Maryland School of Medicine, Baltimore, 21201.
M D
MD
Miliotis
J E
JE
Galen
J B
JB
Kaper
J G
JG
Morris
D001483:Base Sequence; D002460:Cell Line; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D005516:Food Microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011237:Predictive Value of Tests; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
D015342:DNA Probes; D004269:DNA, Bacterial