[Cytotoxicity of chloramphenicol on cultured mammalian cells].
Cytotoxicity of chloramphenicol on cultured mammalian cells was studied. Growth of V79 cells, derived from Chinese hamster lung was inhibited by treatment with chloramphenicol at above 300 micrograms/ml for 24 to 48 hr. Inhibitory effect on survival of V79 cells was not observed in cultures treated with chloramphenicol at 300 to 1,000 micrograms/ml for 2 to 12 hr. Meanwhile, treatment with chloramphenicol at 3,000 micrograms/ml for 2 to 24 hr elicited a dose-related reduction of cell survival. Inhibition of cell survival was also observed in cultures treated with 300 to 3,000 micrograms/ml for 24 hr in a treatment time-dependent manner. Exposure of cells to chloramphenicol at 300 to 3,000 micrograms/ml for 2 hr resulted in an inhibition of DNA-, RNA-, and protein-syntheses of the cells, in a dose-related fashion.
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Mamada
D000818:Animals; D002460:Cell Line; D002701:Chloramphenicol / Q000633:toxicity*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug
D002701:Chloramphenicol