Human oligodendrocytes in dissociated cell culture.
Human oligodendrocytes have been successfully maintained in cell cultures for 14 weeks using a modification of a method used previously for animal brain cell cultures. Dissociated cell cultures from spinal cords of human foetuses of 10 to 20 weeks gestional age were investigated for up to 98 days. Oligodendrocytes were identified by monoclonal human antiserum specific for myelin-associated glycoprotein, by polyclonal rabbit antiserum against myelin basic protein, and by the mouse monoclonal antibody I6G1. Astrocytes were identified by polyclonal antibodies against glial fibrillary acidic protein. Immunocytochemical cell identification was corroborated by electron microscopy, by which glial cells were investigated both in situ and in culture. Immunocytochemical staining of myelin-associated glycoprotein showed specifically labelled oligodendrocytes on electron microscopy. The present study indicates that human oligodendrocytes, a putative target in demyelinating disease, can be studied in dissociated cell culture of human foetal spinal cord for several weeks in vitro under stable conditions.
Rihs
F
F
Neurologische Universitätsklinik, Inselspital, Bern, Switzerland.
Kesselring
J
J
Meier
C
C
D001253:Astrocytes / Q000166:cytology; D002478:Cells, Cultured; D005333:Fetus / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology* / Q000648:ultrastructure