Assessment of membrane protection by 31P-NMR effects of lidocaine on calcium-paradox in myocardium.
In studying calcium paradox, perfused rat hearts were used to investigate the myocardial protective effects of lidocaine. Intracellular contents of phosphates were measured using the 31P-NMR method. In hearts reexposed to calcium, following 3 minute calcium-free perfusion, a rapid contracture occurred, followed by rapid and complete disappearance of intracellular phosphates with no resumption of cardiac function. In hearts where lidocaine was administered from the onset of the calcium-free perfusion until 2 minutes following the onset of reexposure to calcium, both intracellular phosphates and cardiac contractility were maintained. Therefore, it can be said that cell membranes were protected by lidocaine.
Sakai
H
H
First Department of Internal Medicine, Osaka City University Medical School, Japan.
Yoshiyama
M
M
Teragaki
M
M
Takeuchi
K
K
Takeda
T
T
Ikata
M
M
Ishikawa
M
M
Miura
I
I
D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D004734:Energy Metabolism / Q000187:drug effects; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008012:Lidocaine / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D010758:Phosphorus; D051381:Rats; D011921:Rats, Inbred WKY
D010758:Phosphorus; D008012:Lidocaine; D002118:Calcium