Standardization of tritium-sensitive film for quantitative autoradiography.
A method for the calibration of plastic tritium standards for use with LKB Ultrofilm is described and validated. This method uses 12-microns cryostat slices of frozen liver which have been labeled with [3H]-formaldehyde and extracted with chloroform-methanol to remove lipids. Quantitative autoradiographic measurement of 3H radioactivity in the liver slices was underestimated by 35% when lipids were not extracted. Plastic sections impregnated with tritium were calibrated in terms of lipid-extracted, tissue-equivalent radioactivity content. Calibrated standard curves for these plastic standards were closely fit (p = 0.99) by second order polynomial equations for exposures of 1, 4, 7, 13, 28, and 104 days. The equations are generally useful for any plastic tritium standards.
Baskin
D G
DG
Veterans Administration Medical Center, Seattle, Washington 98108.
Filuk
P E
PE
Stahl
W L
WL
D000818:Animals; D001345:Autoradiography / Q000295:instrumentation* / Q000379:methods; D002725:Chloroform; D005557:Formaldehyde; D005629:Frozen Sections; D050356:Lipid Metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D008433:Mathematics; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014962:X-Ray Film / Q000592:standards*
D014316:Tritium; D005557:Formaldehyde; D002725:Chloroform