Heat shock mRNA in mouse epidermis after UV irradiation.
Total RNA from murine epidermis was extracted at different times after irradiation with erythemogenic doses of ultraviolet light (UVB or UVB/UVA) and hybridized to a DNA probe from the gene of a heat shock protein (hsp 70). An intense and transitory enrichment in RNA molecules hybridizing to the DNA probe was found between 15 and 120 min after irradiation, followed by a return to control levels over the next 70 h. Dose-response analysis indicates that 30 min after the irradiation, the relative amount of RNA hybridizing to the hsp 70 DNA probe increases with the dose (within the range explored: 0-180 mJ/cm2) up to values greater than 5 times the control.
Laboratoires de Recherche Fondamentale de l'Oréal, Aulnay-sous-Bois, France.
S
S
Brunet
P U
PU
Giacomoni
D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D004307:Dose-Response Relationship, Radiation; D004587:Electrophoresis, Agar Gel; D006360:Heat-Shock Proteins / Q000235:genetics / Q000528:radiation effects*; D006801:Humans; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D012333:RNA, Messenger / Q000096:biosynthesis*; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
D015342:DNA Probes; D006360:Heat-Shock Proteins; D012333:RNA, Messenger