Adsorption chromatography on cellulose. IV. Separation of D- and L-tryptophan and D- and L-methyltryptophan on cellulose with aqueous solvents.
Differences in RF values between D- and L-tryptophan on cellulose paper, developed with water, were observed already in 1954 and now several variables of this separation, such as modifications to the tryptophan molecule, the temperature and salting-out have been examined. Differences were found in the mechanism of the process compared with the systems described by Yuasa et al. [Chromatographia, 21 (1986) 79], who separated amino acid enantiomers on cellulose with pyridine-ethanol-water mixtures.
Institut de Chimie Minérale et Analytique, Université de Lausanne, Switzerland.
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Lederer
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D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D012997:Solvents; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000302:isolation & purification*
D012997:Solvents; C028129:tryptophan methyl ester; D014364:Tryptophan