Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of organophosphate pesticides.
A simple and rapid method for isolation of eleven organophosphate pesticides with Sep-Pak C18 cartridges from human urine and plasma, is presented. The detection of the pesticides was made by wide-bore capillary gas chromatography (GC) with flame ionization detection. The pesticide-containing samples, after mixing with water, were directly applied to the cartridges and eluted with chloroform/isopropanol (9:1). The recoveries with use of the cartridges were excellent for most pesticides. Separation of each pesticide peak from each other and from impurities on the gas chromatograms was also satisfactory with use of non-polar and slightly polar capillary columns. The isolation method with use of the cartridges, together with the wide-bore capillary GC, seems very useful in forensic and environmental chemistry and clinical toxicology.
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
J
J
Liu
O
O
Suzuki
T
T
Kumazawa
H
H
Seno
D002849:Chromatography, Gas / Q000295:instrumentation; D006801:Humans; D007306:Insecticides / Q000032:analysis* / Q000097:blood / Q000652:urine; D009943:Organophosphorus Compounds
D007306:Insecticides; D009943:Organophosphorus Compounds