High yields of specific hybridomas obtained by electrofusion of murine lymphocytes immunized in vivo or in vitro.
We have studied the use of electrofusion to obtain hybridomas producing antigen-specific antibodies after immunization of murine lymphocytes in vitro. Under optimal conditions fusion frequencies of the order of magnitude of 10(-3) were obtained, which is approximately 80-fold higher than the mean value obtained with fusion induced by polyethylene glycol. The number of antigen-specific hybridomas was also increased in a comparable way. The high yields of specific hybridomas observed with electrofusion were independent of the immunization procedure, the antigen or the hapten of interest, or the sources of the lymphocytes. The data presented in this paper indicate that electrofusion may be an extremely attractive alternative method for immortalization of human lymphocytes following immunization in vitro.
Laboratory of Biochemistry, Faculty of Medicine, University of Amsterdam, The Netherlands.
G
G
van Duijn
J P
JP
Langedijk
M
M
de Boer
J M
JM
Tager
D000818:Animals; D002459:Cell Fusion / Q000187:drug effects; D004558:Electric Stimulation / Q000379:methods; D005260:Female; D006825:Hybridomas / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000494:pharmacology; D011402:Pronase; D013154:Spleen / Q000276:immunology
D011092:Polyethylene Glycols; D011402:Pronase