cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.
The Ku antigen is a DNA-associated nuclear protein recognized by sera from patients with autoimmune diseases. It consists of two polypeptides of 86 and 70 kDa. cDNA clones encoding the 86-kDa subunit of the Ku antigen were isolated by probing lambda gt11 recombinant cDNA expression libraries with a monoclonal antibody specific for this protein. The amino acid sequence deduced from the cDNA comprises 732 amino acids and corresponds to a protein with molecular weight of 81.914. Nineteen residues at the NH2 terminus determined by protein sequencing corresponded to the sequence deduced from the cDNA. The predicted amino acid sequence contains a region with repeating leucine residues similar to the "leucine zipper" structure observed in the c-myc, v-myc, and c-fos oncogene products. The largest cDNA hybridized to 2.7- and 3.4-kilobase poly(A)+ mRNAs from HeLa cells. The cDNA clones expressed fusion proteins immunoreactive with the monoclonal antibody and sera from patients with autoimmune diseases.
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030.
M
M
Yaneva
J
J
Wen
A
A
Ayala
R
R
Cook
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005333:Fetus; D006801:Humans; D000072200:Ku Autoantigen; D008099:Liver / Q000032:analysis; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D011487:Protein Conformation; D015183:Restriction Mapping
D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D000954:Antigens, Surface; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D004247:DNA; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen