Demonstration of functional acetylcholinesterase on the soma of individual neurones of Aplysia by in vivo microspectrophotometry.
The presence of functional acetylcholinesterase is demonstrated in vivo on somatic membranes of single ganglionic neurones of Aplysia using concurrently microspectrophotometry and electrophysiology. The similarity of the effects of an irreversible blocker of acetylcholinesterase and of phospholipase C from Bacillus cereus suggests that acetylcholinesterase is anchored in the membrane via phosphatidylinositol.
Laboratoire de Neurobiologie Cellulaire et Moléculaire, C.N.R.S. Gif sur Yvette, France.
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D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001048:Aplysia / Q000201:enzymology*; D002217:Carbachol / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005724:Ganglia / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D009946:Organothiophosphorus Compounds / Q000494:pharmacology; D013053:Spectrophotometry
D004791:Enzyme Inhibitors; D009946:Organothiophosphorus Compounds; C051451:methylphosphonothiolate; D002217:Carbachol; D000110:Acetylcholinesterase