Chloroplast ribosomal protein gene rps12 of Chlamydomonas reinhardtii. Wild-type sequence, mutation to streptomycin resistance and dependence, and function in Escherichia coli.
The chloroplast rps12 gene encoding ribosomal protein S12 from wild-type as well as from streptomycin-resistant and -dependent mutants of Chlamydomonas reinhardtii was cloned and sequenced. At least six mRNA transcripts ranging in size from 800 to 4800 nucleotides were found to contain the rps12 coding sequence. As in Escherichia coli and Euglena, the C. reinhardtii rps12 gene is continuous, in contrast to its trans-spliced structure in higher plants. However, rps12 of C. reinhardtii is no longer immediately adjacent to the rps7 gene encoding ribosomal protein S7 as it is in other organisms. The deduced amino acid sequence of C. reinhardtii S12 protein shows strong homologies (48-79% identity) to S12 protein sequences of other organisms, but it has extra amino acid residues at its C terminus. Single base pair changes at different sites result in streptomycin-resistant or -dependent mutants with amino acid changes identical to comparable mutations in the E. coli S12 protein. The chloroplast rps12 gene is also expressed under the control of its own promoter in E. coli cells, and the C. reinhardtii S12 protein produced assembles into the E. coli ribosomes where it appears to function efficiently.
Department of Botany, Duke University, Durham, North Carolina 27706.
X Q
XQ
Liu
N W
NW
Gillham
J E
JE
Boynton
D000595:Amino Acid Sequence; D001483:Base Sequence; D002696:Chlamydomonas / Q000187:drug effects / Q000235:genetics*; D002736:Chloroplasts / Q000378:metabolism*; D004351:Drug Resistance / Q000235:genetics; D005796:Genes; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D009876:Operon; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013307:Streptomycin / Q000494:pharmacology*
D015345:Oligonucleotide Probes; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12; D013307:Streptomycin