Lactate dehydrogenase from adult Setaria digitata (Nematoda: Onchocercidae).
Lactate dehydrogenase of Setaria digitata exhibited an optimum pH of 7.0-8.0 and showed resistance to high temperature. The inhibition/activation of various anions differed in both the forward and backward directions. Filarin (a drug used in Siddha medicine) and diethylcarbamazine (DEC) inhibited pyruvate reduction rather than lactate oxidation. High pyruvate reduction:lactate oxidation at Vmax and Vmax/Km favoured pyruvate reduction in vivo. The enzyme exists as isozymes (four in the female and three in the male) and their separation depended on the percentage of gel and on pH. The mobility of the 700 X g supernatant fraction in the gel was less than that of the 10,000 X g supernatant.
Department of Zoology, University of Madras, Guindy Campus, India.
M J
MJ
Banu
K
K
Nellaiappan
S
S
Dhandayuthapani
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D004589:Electrophoresis, Disc; D005260:Female; D005370:Filarioidea / Q000201:enzymology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000037:antagonists & inhibitors; D007773:Lactates / Q000378:metabolism; D008297:Male; D011773:Pyruvates / Q000378:metabolism; D012719:Setariasis / Q000469:parasitology / Q000662:veterinary
D007527:Isoenzymes; D007773:Lactates; D011773:Pyruvates; D007770:L-Lactate Dehydrogenase