An adhesion-hemadsorption inhibition test for the detection of serum antibody to Mycoplasma gallisepticum.
A simple adhesion-hemadsorption inhibition (AHAI) test was developed for the detection of antibodies to Mycoplasma gallisepticum in the chicken sera. The AHAI antibody was detected simultaneously with HI antibody from sera of chickens intratracheally inoculated with viable cells of M. gallisepticum. A good correlation between HI and AHAI antibody titers was obtained with 382 (84.7%) of 451 sera from chickens reared on farms spontaneously contaminated with M. gallisepticum, whereas the remainder, 69 sera, was positive for HI but negative for AHAI test. It was not apparent whether the latters exhibited a non-specific reaction or the discrepancy was due to the lower sensitivity of AHAI reaction. The AHAI test does not require a great amount of antigen, special reagents or instruments, or pre-absorption treatment of test sera, and, therefore, it may serve as a simple serological test for detecting antibodies to M. gallisepticum.
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Shimizu
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Nagatomo
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002645:Chickens; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary*; D008297:Male; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology*
D000907:Antibodies, Bacterial