Altered metabolism of poly(ADP-ribose) in the peripheral blood lymphocytes of patients with systemic lupus erythematosus.
A method was developed to measure poly(ADP-ribose) metabolism in peripheral blood lymphocytes. The technique involved the isolation of lymphocytes on Ficoll gradients, followed by lysis with 5M NaCl. The synthesis and degradation of poly(ADP-ribose) in this crude lysate, measured by the incorporation of 3H-labeled NAD into acid-precipitable counts, was compared in 18 patients with systemic lupus erythematosus (SLE), 10 patients with rheumatoid arthritis, and in 10 control patients without rheumatoid arthritis. Patients with SLE showed a 70% decrease in poly(ADP-ribose) synthesis (P less than 0.001); this decreased synthesis persisted even with the addition of histones or DNase. We present possible explanations of the role of poly(ADP-ribose) in SLE.
Department of Medicine, University of Saskatchewan, Saskatoon, Canada.
J T
JT
Sibley
B L
BL
Haug
J S
JS
Lee
D000293:Adolescent; D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009702:Nucleoside Diphosphate Sugars / Q000097:blood*; D011064:Poly Adenosine Diphosphate Ribose / Q000097:blood* / Q000502:physiology
D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose