The Streptomyces K15 DD-peptidase/penicillin-binding protein. Active site and sequence of the N-terminal region.
The N-terminal region of the Streptomyces K15 DD-peptidase/penicillin-binding protein shows high homology with that of other penicillin-interactive proteins or domains. The active-site serine residue of the conserved tetrad Ser-Xaa-Xaa-Lys occurs at position 35. There is no indication for the presence of a signal peptide or an N-terminal hydrophobic sequence, suggesting that the Streptomyces K15 enzyme is probably anchored to the membrane by a C-terminal peptide segment.
Service de Microbiologie, Université de Liège, Institut de Chimie, Belgium.
M
M
Leyh-Bouille
J
J
Van Beeumen
S
S
Renier-Pirlot
B
B
Joris
M
M
Nguyen-Distèche
J M
JM
Ghuysen
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001665:Binding Sites; D002352:Carrier Proteins; D006602:Hexosyltransferases; D008969:Molecular Sequence Data; D002267:Muramoylpentapeptide Carboxypeptidase; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010446:Peptide Fragments; D010458:Peptidyl Transferases; D013302:Streptomyces / Q000201:enzymology*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010446:Peptide Fragments; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase