Development of a diagnostic test for Johne's disease using a DNA hybridization probe.
A DNA probe, M13 mpHAW71, that detects Mycobacterium paratuberculosis in the fecal material of infected animals was developed for use in the diagnosis of Johne's disease. The probe detected as few as 10(5) M. paratuberculosis when hybridized under stringent conditions to total genomic DNA purified from bovine fecal material. When the probe was used diagnostically, it did not differentiate members of the Mycobacterium avium-M. intracellulare-M. paratuberculosis complex. Compared with culturing, the DNA probe identified 34.4% more mycobacterium-containing fecal samples, and testing took only 72 h to complete.
Department of Natural Resources, Madison, Wisconsin 53707.
S S
SS
Hurley
G A
GA
Splitter
R A
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Welch
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D003429:Cross Reactions; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D003720:Densitometry; D005243:Feces / Q000382:microbiology; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D010283:Paratuberculosis / Q000175:diagnosis* / Q000382:microbiology; D014162:Transfection
D015342:DNA Probes; D004269:DNA, Bacterial