Formation of alanine and glutamine in chick (Gallus domesticus) skeletal muscle.
1. Incubation of extensor digitorum communis muscles from fed chicks in the presence of plasma concentrations of branched-chain amino acids (BCAA) increased the formation of glutamate, glutamine and alanine. This effect was inhibited by 1.5 mM L-cycloserine. 2. 2-Oxoisocaproate (0.1 and 0.5 mM) increased the formation of leucine but decreased that of glutamate, glutamine and alanine. 3. NH4Cl (0.3 mM) increased the formation of glutamine, and decreased the release and intracellular concentrations of glutamate and alanine. 4. Our results demonstrate that alanine and glutamine are synthesized de novo in chick skeletal muscle and demonstrate the similarity in alanine and glutamine synthesis in skeletal muscle between the domestic fowl and mammals.
Department of Animal Science, University of Alberta, Edmonton, Canada.
G
G
Wu
J R
JR
Thompson
G W
GW
Sedgwick
M
M
Drury
D000409:Alanine / Q000096:biosynthesis*; D000597:Amino Acids, Branched-Chain / Q000378:metabolism*; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D009132:Muscles / Q000378:metabolism*
D000597:Amino Acids, Branched-Chain; D005971:Glutamates; D005973:Glutamine; D000409:Alanine