Molecular cloning and sequence analysis of cDNA coding for canine gastrin.
We have isolated and sequenced cDNA clones encoding for preprogastrin from a canine antral cDNA library. Comparison of this sequence with those of porcine, human, and rat gastrin reveals extensive (83%) homology in the gastrin coding region as well as short regions of conserved nucleotides in the noncoding regions. The canine sequence encodes a preprogastrin of 104 amino acids which consists of a signal peptide, a 37-amino acid prosegment, and a 34-amino acid gastrin sequence, followed by a glycine (the amide donor), and flanked by pairs of arginine residues.
Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul.
S C
SC
Kang
K L
KL
Agarwal
O J
OJ
Yoo
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004285:Dogs; D005755:Gastrins / Q000032:analysis / Q000235:genetics*; D005815:Genetic Code; D015335:Molecular Probes; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D021382:Protein Sorting Signals / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D005755:Gastrins; D015335:Molecular Probes; D011498:Protein Precursors; D021382:Protein Sorting Signals; D012333:RNA, Messenger; C042873:preprogastrin; D004247:DNA