Hypervariability of simple sequences as a general source for polymorphic DNA markers.
Short simple sequence stretches occur as highly repetitive elements in all eukaryotic genomes and partially also in prokaryotes and eubacteria. They are thought to arise by slippage like events working on randomly occurring internally repetitive sequence stretches. This predicts that they should be generally hypervariable in length. I have used the polymerase chain reaction (PCR) process to show that several randomly chosen simple sequence loci with different nucleotide composition and from different species show extensive length polymorphisms. These simple sequence length polymorphisms (SSLP) may be usefully exploited for identity testing, population studies, linkage analysis and genome mapping.
Max-Planck Institut für Entwicklungsbiologie, Tübingen, FRG.
D
D
Tautz
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D005796:Genes; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012463:Saliva / Q000032:analysis; D012867:Skin / Q000032:analysis; D014907:Whales
D004247:DNA