Enhanced procoagulatory activity (PCA) of human monocytes/macrophages after in vitro stimulation with chemically modified LDL.
In cultured human monocytes/macrophages, surface expression of procoagulatory activity (PCA) was induced by chemically modified LDL (acetyl-LDL and MDA-LDL) in a dose- and time-dependent manner. Maximum PCA (30-fold increase) was detected after 24 h of culture with modified LDL at doses of 25-750 micrograms protein/ml. Using factor VII deficient human plasma and phospholipase C this PCA was identified as tissue thromboplastin activity (factor III). These results suggest a further atherogenic potential for modified LDL through stimulation of the conversion of fibrinogen to fibrin in the atheromatous lesion.
Department of Clinical Chemistry, Georg-August University, Göttingen, F.R.G.
P
P
Schuff-Werner
G
G
Claus
V W
VW
Armstrong
H
H
Köstering
D
D
Seidel
D001779:Blood Coagulation Factors / Q000378:metabolism*; D002478:Cells, Cultured; D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008264:Macrophages / Q000502:physiology*; D009000:Monocytes / Q000502:physiology*
D001779:Blood Coagulation Factors; D004731:Endotoxins; D008077:Lipoproteins, LDL; C032629:acetyl-LDL; C045072:macrophage procoagulant activity