Red blood cell filterability during incubation and its dependence on RBC shape, glucose and serum albumin: a study in the diabetic ob/ob-mouse.
Red blood cell (RBC) filterability in the obese-hyperglycaemic ob/ob-mouse is markedly impaired compared with the lean controls. A new RBC filtration device with improved time resolution was used to study this phenomenon in relation to RBC shape-transformations and the effects of glucose and albumin. In ob/ob-mice, but not in controls, filterability increased with time; however, the difference between the mice remained significant after 2 h. Stomatocytosis (cup shaped RBCs) was in the order of 80-90% in both types of mice and 56% in human blood. The proportion of stomatocytes decreased linearly with time, most rapidly in the ob/ob-mice. Only 1% echinocytes (crenated RBCs) was seen after 2 h of incubation. In the absence of serum albumin stomatocytosis was initially 40% and declined further during incubation. Echinocytes showed the opposite reaction, increasing from about 10% to 55%. Glucose, 20 mM, caused an immediate increase in filterability but had no corresponding effect on the RBC shape transformations.
Department of Histology and Cell Biology, University of Umeå, Sweden.
K G
KG
Engström
I B
IB
Täljedal
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000473:pathology; D004906:Erythrocyte Count / Q000187:drug effects; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004912:Erythrocytes / Q000473:pathology*; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D051379:Mice; D008820:Mice, Obese; D012709:Serum Albumin / Q000494:pharmacology*; D013997:Time Factors
D012709:Serum Albumin; D005947:Glucose