Purification and characterization of a myotoxic phospholipase A2 from Indian cobra (Naja naja naja) venom.
A major phospholipase A2 (NN-XIII-PLA2) which constitutes 20% of the whole Naja naja naja venom was purified to homogeneity on CM-Sephadex C-25 column chromatography. NN-XIII-PLA2 is a basic protein with a mol. wt of 11,200 by SDS-PAGE. This enzyme has low enzymatic activity but is more toxic to mice than the whole venom. The LD50 value (i.p.) of NN-XIII-PLA2 is 2.4 mg/kg body weight (whole venoms LD50 is 2.8 mg/kg body weight). It induces neurotoxic-like signs in experimental animals. It induces myotoxicity when injected i.m. into the thigh muscle of mice and edema when injected into the foot pads of mice. This enzyme has a fluorescence maxima between 310-316 nm which is typical of tyrosine residues.
Department of Studies in Biochemistry, Manasagangothri, University of Mysore, India.
M K
MK
Bhat
T V
TV
Gowda
D000818:Animals; D004487:Edema / Q000139:chemically induced; D004546:Elapid Venoms / Q000032:analysis / Q000633:toxicity*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D051379:Mice; D009132:Muscles / Q000187:drug effects*; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D054467:Phospholipases A2; D013050:Spectrometry, Fluorescence
D004546:Elapid Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2