A perfusion system developed for 31P NMR study of melanoma cells at tissue-like density.
A perfusion culture system has been developed for 31P NMR study of human uveal melanoma metabolism by adapting the Vitafiber I cartridge system (Amicon). 31P NMR spectra collected weekly during periods of up to 10 weeks demonstrated increasing levels of phosphorus metabolites as the anchorage-dependent cells grew to tissue-like density.
Howe Laboratory of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114.
M M
MM
Minichiello
D M
DM
Albert
N H
NH
Kolodny
M S
MS
Lee
J L
JL
Craft
D004867:Equipment Design; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008545:Melanoma / Q000032:analysis* / Q000648:ultrastructure; D010477:Perfusion / Q000295:instrumentation; D010758:Phosphorus; D014407:Tumor Cells, Cultured; D014604:Uveal Neoplasms / Q000032:analysis* / Q000648:ultrastructure
D010758:Phosphorus