Heterogeneous genomic amplification in Streptomyces glaucescens: structure, location and junction sequence analysis.
Certain chromosomal markers in Streptomyces glaucescens behave unstably, being lost at high frequency as a result of extensive genomic deletion. Additionally, mutant strains possessing such deletions frequently display intense DNA amplification. With the help of a wild-type cosmid library we investigated the structure of the amplified DNA sequences (ADS) and the corresponding wild-type amplifiable units of DNA (AUD). The reiterations were heterogeneous in location, copy number and sequences involved and originated predominantly from a single 100 kb region of the chromosome called the AUD locus. All strains bearing reiterations possessed associated deletions which terminated either close to or at the ADS. The termini of four AUDs were sequenced in order to gain more knowledge about these heterogeneous amplifications. In three of the four cases investigated small, interrupted homologies were found bordering the AUDs. With the help of orthogonal-field-alternation gel electrophoresis (OFAGE) we were able to visualize a tandem reiteration of more than 1,500 kb in length.
Häusler
A
A
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Birch
A
A
Krek
W
W
Piret
J
J
Hütter
R
R
D001483:Base Sequence; D002872:Chromosome Deletion; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000235:genetics*
D004269:DNA, Bacterial