Effects of the antioestrogen, ICI 164,384, on oestrogen induced RNAs in MCF-7 cells.
The effects of ICI 164,384 on the expression of six oestrogen-regulated RNAs (pNR-1, pNR-2, pNR-13, pNR-17, pNR-25 and pNR-100) and the 46 kDa secreted protein were measured in MCF-7 cells. In marked contrast to tamoxifen, an antioestrogen commonly used in the treatment of breast cancer, ICI 164,384 administered alone had little or no effect on the RNAs or protein. ICI 164,384 completely inhibited the induction of the RNAs and 46 kDa protein by oestradiol. Although ICI 164,384 has an affinity for the human oestrogen receptor only slightly less than that of oestradiol, half maximal inhibition of oestradiol action was attained with between a 50 and 150-fold molar excess of ICI 164,384. The pNR-1 RNA is induced by tamoxifen but this induction was abolished by ICI 164,384. Thus, ICI 164,384 acts as a potent antioestrogen for the regulation of the expression of specific oestrogen-responsive genes in human breast cancer cells.
University Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, U.K.
L R
LR
Wiseman
A E
AE
Wakeling
F E
FE
May
B R
BR
Westley
D001943:Breast Neoplasms; D002460:Cell Line; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007700:Kinetics; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D053284:Polyunsaturated Alkamides; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000187:drug effects / Q000302:isolation & purification
D004965:Estrogen Antagonists; D009363:Neoplasm Proteins; D053284:Polyunsaturated Alkamides; D012334:RNA, Neoplasm; D004958:Estradiol; C051781:ICI 164384