Screening for thermostability and electrophoretic red blood cell sorbitol dehydrogenase (E.C.1.1.1.14) variants.
A screening for both thermostability and electrophoretic red blood cell sorbitol dehydrogenase (RBC-SORD) variants in blood donors was performed. SORD activity in standard conditions (unheated samples) in 274 individuals was 198 +/- 38.6 mIU/g Hb. The ratio of enzymatic activity after heating (H) to the activity in controls (C) before heating (H/C ratio) was 0.39 +/- 0.10. H/C ratios minor than 0.1 in 3 out of 274 blood donors and higher than 0.9 in 1 were observed. In 208 individuals, four electrophoretic phenotypes were observed: I) Three bands, named a, b and c, with cathodic mobility in 163 individuals (78.36%); II) Two bands a and c in 25 individuals (12.02%); III) Two bands b and c in 14 (6.73%); and IV) One band, c in 6 (2.88%). Studies carried out to characterize the three bands suggest that they are isozymes of the same locus with the observation of an interchange of the bands as a normal phenomena.
División de Genética, Instituto Mexicano del Seguro Social, Jalisco, Mexico.
B
B
Ibarra
G
G
Vaca
F J
FJ
Perea
M
M
Rábago
C
C
Alvarez
C
C
Ortíz
C
C
Medina
D004589:Electrophoresis, Disc; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D007064:L-Iditol 2-Dehydrogenase / Q000032:analysis* / Q000235:genetics; D010641:Phenotype; D013401:Sugar Alcohol Dehydrogenases / Q000032:analysis*; D013696:Temperature
D007527:Isoenzymes; D013401:Sugar Alcohol Dehydrogenases; D007064:L-Iditol 2-Dehydrogenase