High-performance liquid chromatography of proteins on N-methylpyridinium polymer columns.
Two types of 4-methylpyridinium polymers (4VP-DVB-Me and 4VP-EG-Me, cross-linked with divinylbenzene and ethylene glycol dimethacrylate, respectively) were employed for the analysis of proteins in ion-exchange high-performance liquid chromatography. These polymers had different physical properties in the dry state, but showed similar retentions in size-exclusion chromatography using carbohydrate standards. Generally, the 4VP-EG-Me column was superior to the 4VP-DVB-Me column with regard to separation and recovery of proteins.
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
A
A
Sugii
K
K
Harada
K
K
Nishimura
R
R
Hanaoka
S
S
Masuda
D002851:Chromatography, High Pressure Liquid; D011108:Polymers; D011506:Proteins / Q000032:analysis*; D011726:Pyridinium Compounds; D013056:Spectrophotometry, Ultraviolet
D011108:Polymers; D011506:Proteins; D011726:Pyridinium Compounds; C014677:1-methylpyridinium