A comparison of two methods and two additives for faecal cultivation of bovine trichostrongyle larvae.
The larval recovery rates of Ostertagia ostertagi and Cooperia oncophora from monospecific copro-cultures were determined by the methods of Corticelli and Lai, and Henriksen and Korsholm using vermiculite or polystyrene pellets as inert additives. The addition of either vermiculite or polystyrene pellets in the two culture methods significantly (P less than 0.05) improved larval recoveries of both parasite species. Recovery rates with the Henriksen and Korsholm method were higher (P less than 0.05) for both species than with the Corticelli and Lai method. Advantages and disadvantages inherent in the use of the two culture methods with and without additives are discussed.
Royal Veterinary and Agricultural University, Frederiksberg C., Denmark.
P
P
Steffan
S A
SA
Henriksen
P
P
Nansen
D000538:Aluminum Silicates; D000818:Animals; D002417:Cattle; D003470:Culture Media; D005243:Feces / Q000469:parasitology*; D007814:Larva / Q000254:growth & development; D008863:Microspheres; D010028:Ostertagia / Q000254:growth & development*; D011137:Polystyrenes; D014251:Trichostrongyloidea / Q000254:growth & development*
D000538:Aluminum Silicates; D003470:Culture Media; D011137:Polystyrenes; C003760:vermiculite