1H COSY spectra of superfused brain slices of rat: ex vivo direct assignment of resonances.
Realization of 1D 1H spectra of superfused brain slices of rats is described. Two-dimensional 1H-1H COSY spectra have been used for the direct assignment of resonances on living tissue (lactate, GABA, N-acetyl aspartate, aspartate, glutamine/glutamate, creatine/phosphocreatine, taurine, inositol, choline/ethanolamine). Utilization of the SUPERCOSY sequence permits simultaneously the enhancement of off-diagonal signals for small metabolites and the reduction of the signals for water and macromolecules.
Institut de Chimie des Substances Naturelles, CNRS, Gif sur Yvette, France.
B
B
Gillet
S
S
Mergui
J C
JC
Beloeil
J
J
Champagnat
G
G
Fortin
T
T
Jacquin
D000818:Animals; D000831:Animals, Newborn; D001834:Body Water / Q000032:analysis; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D006859:Hydrogen; D007773:Lactates / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010743:Phospholipids / Q000032:analysis; D051381:Rats
D007773:Lactates; D010743:Phospholipids; D006859:Hydrogen