Molecular characterization of a surface protein antigen gene from serotype c Streptococcus mutans, implicated in dental caries.
The complete nucleotide sequence of the gene for a cell-surface protein antigen (PAc) of Streptococcus mutans MT8148 (serotype c) was determined. The pac gene consisted of 4695 bp and coded for a 170773D protein. The pac gene product contained a putative 38 amino acid signal peptide, resulting in a 166817D mature protein. A potential promoter sequence and a putative Shine-Dalgarno sequence preceded the open reading frame. Two internal repeating amino acid sequences were present in the PAc. One repeating region located in the N-terminal region was rich in alanine, and the other located in the central region was rich in proline. Southern blot analysis under the less stringent condition (allowing up to 35% base mismatch) revealed that the probe covering the proline-rich region hybridized to DNA preparations from strains of Streptococcus cricetus, Streptococcus sobrinus and Streptococcus downei as well as Streptococcus mutans.
Okahashi
N
N
Department of Dental Research, National Institute of Health, Tokyo, Japan.
Sasakawa
C
C
Yoshikawa
M
M
Hamada
S
S
Koga
T
T
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015139:Blotting, Southern; D003062:Codon; D004269:DNA, Bacterial / Q000235:genetics*; D003731:Dental Caries / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000235:genetics; D015183:Restriction Mapping; D012703:Serotyping; D013295:Streptococcus mutans / Q000145:classification / Q000235:genetics* / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D003062:Codon; D004269:DNA, Bacterial; D008565:Membrane Proteins; D021382:Protein Sorting Signals