Spectrophotometric method for differentiation of cardiac and hepatic lactate dehydrogenase activities in dogs.
To differentiate the origin of high total lactate dehydrogenase (LD) activity in canine sera, a spectrophotometric method based on the preferential inhibition of cardiac LD isoenzymes by pyruvate was performed. Comparison with the electrophoretic separation of LD isoenzyme activities and determination of the hydroxybutyrate dehydrogenase-to-LD ratio indicated that the method proposed gave a better discrimination between cardiac and hepatic LD activities than did the other tests.
Departmento de Patologia Clinica Veterinaria, Universidad de Córdoba, Spain.
E
E
Aguilera-Tejero
R
R
Mayer-Valor
G
G
Gómez-Cárdenas
D000818:Animals; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008107:Liver Diseases / Q000201:enzymology / Q000662:veterinary*; D009203:Myocardial Infarction / Q000201:enzymology / Q000662:veterinary; D009206:Myocardium / Q000201:enzymology*; D009336:Necrosis; D013053:Spectrophotometry / Q000379:methods
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase