An immunoprotective monoclonal antibody directed against Leptospira interrogans serovar copenhageni.
A monoclonal antibody (mAb) was prepared by hybridoma technology in BALB/c mice immunized to Leptospira interrogans serovar copenhageni. This mAb agglutinated serovars copenhageni and icterohaemorrhagiae to high titres and protected hamsters, dogs and monkeys against challenge with a virulent strain of serovar copenhageni. The mAb gave protection to hamsters at dilutions up to 1 in 1000; at a 1 in 10 dilution the protective effect lasted for at least two weeks. Biochemical analysis by SDS-PAGE and Western blotting indicated that this mAb reacted with an epitope of a carbohydrate nature.
Schoone
G J
GJ
N. H. Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
Everard
C O
CO
Korver
H
H
Carrington
D G
DG
Inniss
V A
VA
Baulu
J
J
Terpstra
W J
WJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004285:Dogs; D006825:Hybridomas / Q000276:immunology; D007116:Immunization, Passive; D007921:Leptospira interrogans / Q000276:immunology*; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal