Development of a monoclonal antibody for use as an amboceptor in complement fixation tests.
A monoclonal antibody for use as a haemolytic amboceptor in complement fixation tests was developed. The monoclonal antibody selected was investigated in parallel with conventional rabbit amboceptor. The results with the monoclonal amboceptor were at least as good as with the rabbit amboceptor. Production of the monoclonal amboceptor was attained provided that hybridoma growth factor was added to the culture medium.
Laboratory of Virology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
J M
JM
Ossewaarde
A D
AD
de Booij
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002999:Clone Cells / Q000378:metabolism; D003168:Complement Fixation Tests / Q000379:methods; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006461:Hemolysis; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D012756:Sheep
D000911:Antibodies, Monoclonal