[Determination of the endotoxin content of egg products using a miniaturized chromogenic Limulus test].
A chromogenic Limulus amoebocyte lysate assay was applied to monitor endotoxin concentration in egg products. Analysis of differently contaminated whole egg probes revealed a strong correlation of endotoxin concentration to total bacterial count 6 x 10(4) cfu x ng-1, where cfu = colony-forming unit) as well as to number of Enterobacteriaceae (1 ng/7 x 10(2) cfu). Similar relations were also found for egg white and egg yolk probes. A significant influence of heat pretreatment of egg probes (65 degrees C, 60 min) on endotoxin detection could be excluded. Up to a concentration of 10 mg x ml-1 endotoxin-free whole egg material did not interfere with the test system. A miniaturized version of the chromogenic Limulus test, which can be carried out in microtiter plates, is described.
Deutsches Institut für Lebensmitteltechnik, Bundesrepublik Deutschland.
K
K
Steffens
T
T
Maier
D000818:Animals; D015169:Colony Count, Microbial; D004531:Eggs / Q000032:analysis*; D004731:Endotoxins / Q000032:analysis*; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D008033:Limulus Test / Q000379:methods*
D004731:Endotoxins
Bestimmung des Endotoxin-Gehalts in Eiprodukten mit Hilfe eines miniaturisierten chromogenen Limulus-Tests.