Bradykinin-induced translocation of protein kinases C in neuroblastoma NCB-20 cell: dependence on 1,2-diacylglycerol content and free calcium.
Bradykinin (BK)-induced production of 1,2-diacylglycerol (1,2-DG) and translocation of protein kinases C (PKCs) were examined in neuroblastoma-derived hybrid NCB-20 cells. Mass analysis of 1,2-DG exhibited a biphasic increase by 1 microM BK stimulation: the first transient phase and the second broad sustained phase. Among three subspecies of PKC expressed in these cells, types II and III were observed to translocate from cytosol to membrane in response to BK as well as PBt2 by Western blotting analysis. Type II translocated more rapidly and distinctly than type III. However, after treatment with quin 2/AM, the second phase of 1,2-DG formation completely disappeared and PKCs translocation by BK or PBt2 was completely abolished. BK-induced IP3 (1,4,5) formation was temporally consistent with the first transient phase of 1,2-DG formation. These findings suggest that PKCs translocation by BK stimulation is caused by 1,2-DG produced not only via phosphoinositide metabolism, but via other phospholipid breakdown which is Ca2+-dependent.
Department of Biochemistry, Gifu University School of Medicine, Japan.
T
T
Fu
Y
Y
Okano
M
M
Hagiwara
H
H
Hidaka
Y
Y
Nozawa
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology*; D002451:Cell Compartmentation / Q000187:drug effects; D004075:Diglycerides / Q000502:physiology*; D005989:Glycerides / Q000502:physiology*; D006801:Humans; D006822:Hybrid Cells; D007527:Isoenzymes / Q000378:metabolism; D009447:Neuroblastoma / Q000201:enzymology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D011744:Pyrimidinones / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D004075:Diglycerides; D005989:Glycerides; D007527:Isoenzymes; D011744:Pyrimidinones; D013844:Thiazoles; D015240:Phorbol 12,13-Dibutyrate; C047505:R 59022; D011493:Protein Kinase C; D001920:Bradykinin; D002118:Calcium