Growth, flow cytometric, and karyological characterization of L1210 cell sublines resistant to various Pt derivatives.
Growth, flow cytometric, and karyological characteristics were analyzed with respect to differences between sensitive L1210 cells and eight sublines resistant to cisplatin (DDP), 1,2-diaminocyclohexane(DACH)-Pt(II) citrate, DACH-Pt(II) glucarate, cis-dichloro-bis-(isopropylamine)-trans-dihydroxyplatinum (IV) (CHIP, iproplatin), and methotrexate (MTX). No great differences were found in growth properties. The sensitive and a majority of resistant sublines displayed similar flow cytometric and karyological characteristics, major differences were found only in the sublines resistant to CHIP. The relationship between the mechanism(s) of resistance and different pharmacokinetics of the drugs is discussed with special reference to DNA content and chromosome structure.
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
M
M
Hrubisko
B
B
Kysela
P
P
Kuliffay
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D002945:Cisplatin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000032:analysis; D004351:Drug Resistance; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D007621:Karyotyping; D007939:Leukemia L1210 / Q000473:pathology*; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D009944:Organoplatinum Compounds / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D009944:Organoplatinum Compounds; C023359:iproplatin; D002945:Cisplatin; D008727:Methotrexate