Amino acid sequence of pheromone-biosynthesis-activating neuropeptide (PBAN) of the silkworm, Bombyx mori.
We have isolated two distinct pheromone-biosynthesis-activating neuropeptides (PBAN), named PBAN-I and -II, as fully oxidized forms of Met residues from adult heads of the silkworm, Bombyx mori. PBAN-I was identical with the PBAN which we had isolated before. The complete amino acid sequence of PBAN-I, a total of 33 amino acid residues, was determined as H-Leu-Ser-Glu-Asp-Met-Pro-Ala-Thr-Pro-Ala-Asp-Gln-Glu-Met-Tyr-Gln-Pro-As p-Pro- Glu-Glu-Met-Glu-Ser-Arg-Thr-Arg-Tyr-Phe-Ser-Pro-Arg-Leu-NH2. Synthetic PBAN-I after oxidation with H2O2 was chromatographically identical with the isolated PBAN-I. Examination of PBAN activity of synthetic analogues indicated that the carboxyl-terminal portion of PBAN-I was important for biological activity.
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
A
A
Kitamura
H
H
Nagasawa
H
H
Kataoka
T
T
Inoue
S
S
Matsumoto
T
T
Ando
A
A
Suzuki
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D010675:Pheromones / Q000378:metabolism*; D012724:Sex Attractants / Q000378:metabolism*; D013329:Structure-Activity Relationship
D009479:Neuropeptides; D010675:Pheromones; D012724:Sex Attractants; C060896:pheromone-biosynthesis-activating neuropeptide I