Use of cell cultures for predicting the biological effects of mycotoxins.
The risk presented by mycotoxins is a toxicological problem. As the data given by physico-chemical analysis may be difficult to translate in terms of toxicity, however, especially when considering multiple contamination, we have developed a system for toxicological analysis of mycotoxins using cell cultures of different origins. The response of several cell types to a number of well defined mycotoxins was obtained in three days. This approach allowed us to: demonstrate and quantify a toxic effect, define some organ specificity related to the preferential action on a particular cell type, and detect an immunosuppressive effect. The results indicate that the system can be used for toxicological screening and that it has a predictive value for the pathological effects of tested products.
Laboratoire de Pathologie Cellulaire de l'ER 278 (Reseau de toxicologie nutritionnelle MRES), Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
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Robbana-Barnat
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Lafarge-Frayssinet
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Frayssinet
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology; D013605:T-2 Toxin / Q000494:pharmacology; D014255:Trichothecenes / Q000494:pharmacology
D009183:Mycotoxins; D014255:Trichothecenes; D013605:T-2 Toxin