The effect of ascorbic acid on the metabolism of rat calvarial bone cells in vitro.
Addition of 50 micrograms/ml sodium ascorbate to confluent cultures of isolated rat calvarium bone cells resulted in a 21% increase in DNA production, a 50-60% increase in incorporation of [14C]proline into collagenous and noncollagenous proteins, and a 200% increase in alkaline phosphatase activity; under identical conditions, [35S]sulfate incorporation into proteoglycans (glycosaminoglycans) was not affected. These results suggest that ascorbate may be important in maintaining or stimulating the osteogenic phenotype of normal bone cells.
McKay Laboratories of Orthopaedic Research, University of Pennsylvania School of Medicine, Philadelphia 19104-6081.
K P
KP
Spindler
D B
DB
Shapiro
S B
SB
Gross
C T
CT
Brighton
C C
CC
Clark
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000096:biosynthesis; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D006025:Glycosaminoglycans; D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid