Detection of leptospires in pig kidney using DNA hybridisation.
DNA hybridisation detected leptospiral organisms in homogenised kidneys from experimentally infected pigs, and in homogenates of pig kidneys collected at abattoirs. The technique is easy to perform and had some advantages over cultural and histological methods, in permitting the rapid survey of many kidneys simultaneously. Leptospires added to a homogenate of uninfected kidney could be detected at 10(2) organisms ml-1 by DNA hybridisation, but the technique appeared to be less sensitive than culture.
Department of Agriculture and Rural Affairs, Regional Veterinary Laboratory, Bendigo, Victoria, Australia.
B M
BM
McCormick
B D
BD
Millar
R P
RP
Monckton
R T
RT
Jones
R J
RJ
Chappel
B
B
Adler
D000003:Abattoirs; D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis*; D007668:Kidney / Q000382:microbiology*; D007921:Leptospira interrogans / Q000235:genetics / Q000302:isolation & purification*; D007922:Leptospirosis / Q000175:diagnosis / Q000662:veterinary*; D009693:Nucleic Acid Hybridization; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D004269:DNA, Bacterial