Separation of bovine lymphocytes and granulocytes from blood by use of elutriation.
Bovine blood mononuclear cells were separated into 2 fractions by use of centrifugal elutriation. Total recovery, as well as recovery of each fraction, was greater than that obtained by use of Ficoll-sodium diatrizoate separation. The lymphocyte fraction contained less than 1% granulocytes, and the granulocyte fraction contained only 7% lymphocyte contamination. The technique was reproducible and results proved to be comparable with those of Ficoll-sodium diatrizoate density-gradient centrifugation; furthermore, the method is considerably cheaper and less time-consuming for processing large volumes of blood. Viability of cells separated by elutriation always was greater than 98%, whereas viability of cells separated by Ficoll-sodium diatrizaote was greater than 95%. Also, mitogen activation of lymphocytes separated by elutriation was superior to that of lymphocytes separated by Ficoll-sodium diatrizoate centrifugation.
Department of Veterinary Science, North Dakota State University of Agriculture and Applied Science, Fargo 58105.
N
N
Khansari
K
K
Beauclair
T
T
Gustad
D000818:Animals; D002417:Cattle / Q000097:blood*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002498:Centrifugation; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology*; D011897:Random Allocation