Autoradiographic investigations on cell shape-mediated growth regulation of lens epithelial cells in culture.
An autoradiographic method is described which is well suited for the determination of the labelling index in flattened as well as rounded cells. Using this method DNA synthesis of lens epithelial cells in culture was found to be dependent on cell attachment, cell flattening and intact microfilaments. Thus previous results on cell shape mediated growth regulation could be confirmed. Moreover, considering the labelling index it was possible to conclude that cell rounding or a disintegration of microfilaments did not impair ongoing DNA synthesis but did prevent cells from entering the S-phase of the cycle.
Institute of Biochemistry, Martin Luther University, Halle, DDR.
M
M
Iwig
D
D
Ngoli
D
D
Glaesser
D000818:Animals; D001345:Autoradiography / Q000379:methods; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004261:DNA Replication; D004847:Epithelial Cells; D007700:Kinetics; D007908:Lens, Crystalline / Q000166:cytology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D013936:Thymidine