Detection of rabies virus RNA in the central nervous system of experimentally infected mice using in situ hybridization with RNA probes.
Rabies virus is usually demonstrated in human or animal tissues using antigen-detection or viral isolation techniques. Rabies virus RNA can be demonstrated in paraffin-embedded tissues using in situ hybridization. Negative (-) sense 35S- and 3H-labeled RNA probes, specific for rabies virus nucleocapsid protein mRNA, were used for the detection of rabies virus RNA in the nervous system of mice experimentally infected with fixed and street strains of rabies virus. In situ hybridization signals were compared with rabies virus antigen demonstrated with immunoperoxidase staining. Rabies virus RNA and antigen were also demonstrated in the same neurons using a double-labeling technique. In situ hybridization has potential applications as a diagnostic test for rabies and in studies of rabies pathogenesis.
Department of Medicine, Queen's University, Kingston, Ontario, Canada.
A C
AC
Jackson
D L
DL
Reimer
W H
WH
Wunner
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001923:Brain Chemistry; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012367:RNA, Viral / Q000032:analysis*; D011818:Rabies / Q000382:microbiology* / Q000473:pathology; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology; D013116:Spinal Cord / Q000032:analysis* / Q000382:microbiology / Q000473:pathology
D000956:Antigens, Viral; D015347:RNA Probes; D012367:RNA, Viral