[Determination of the amino acid composition of cartilage and creatine phosphokinase isoenzymes with the use of phenylthiocarbamyl amino acid derivatives].
A method for estimation of amino acid composition and collagen-specific amino acids was developed. The assay was based on the precolumn reaction of amino acids with phenylisothiocyanate. The phenylthiocarbamyl derivatives were analyzed by means of reverse-phase chromatography on octadecyl sorbents, which involved gradient elution with ethanol and sodium or ammonium acetate. The method was used for effective separation of collagen-specific and main amino acids in cartilage hydrolysate from healthy persons and of patients with funnel chest and Ehlers-Danlos syndrome and also for determination of amino acid composition in creatine phosphokinase B- and M-subunits.
F E
FE
Romantsev
V N
VN
Prozorovskiĭ
E A
EA
Kozlov
D A
DA
Karpov
E L
EL
Trebukhina
D000596:Amino Acids / Q000032:analysis*; D002356:Cartilage / Q000032:analysis* / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D003402:Creatine Kinase / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D007527:Isoenzymes; D010670:Phenylthiourea; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D007202:Indicators and Reagents; D007527:Isoenzymes; D010670:Phenylthiourea; D003402:Creatine Kinase
Opredelenie aminokislotnogo sostava khriashchevoĭ tkani i izofermentov kreatinfosfokinazy s ispol'zovaniem feniltiokarbamil'nykh proizvodnykh aminokislot.