Preliminary crystallographic analysis of a plant pathogenic factor: pectate lyase.
Pectate lyase is a saccharide-binding enzyme that lyitically depolymerizes polypectate in higher plant cell walls, thus causing soft-rot diseases in food crops. A pectate lyase from Erwinia chrysanthemi, EC16 (PLe), crystallizes in the orthorhombic space group P2(1)2(1)2(1) with unit cell dimension of a = 39.0 A, b = 91.0 A and c = 103.4 A. The asymmetric unit consists of one molecule with a molecular mass of 38,118 daltons and the X-ray diffraction extends to a resolution of 1.8 A. The crystals reproducibly grow to large dimensions and are suitable for a high-resolution X-ray diffraction analysis.
Kim
C Y
CY
Department of Biochemistry, University of California, Riverside 92521.
Mosser
V
V
Keen
N
N
Jurnak
F
F
D010940:Plant Proteins; D011133:Polysaccharide-Lyases; D014961:X-Ray Diffraction
D010940:Plant Proteins; D011133:Polysaccharide-Lyases; C023911:pectate lyase