Increased level of apolipoprotein B mRNA in the liver of ventromedial hypothalamus lesioned obese rats.
The mRNA level of apolipoprotein B (apoB), which is a principal protein component of nascent very low density lipoprotein (VLDL), was determined in parallel with the measurement of acetyl-coenzyme A (Ac-CoA) carboxylase activity in the liver of ventromedial hypothalamus (VMH) lesioned obese rats. Eight weeks after the electrolysis of the bilateral VMH, the level of apoB mRNA in the VMH-lesioned rats was about 1.5-fold higher than that in the sham-operated rats, indicating increased apoB synthesis in the liver of the VMH-lesioned obese rats. The activity of Ac-CoA carboxylase, which is a rate-limiting enzyme for the fatty acid biosynthesis, was about 1.8-fold higher in the VMH-lesioned rats. These observations indicated that VLDL synthesis is increased in the liver of VMH-lesioned obese rats.
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Y
Y
Inui
S
S
Kawata
Y
Y
Matsuzawa
K
K
Tokunaga
S
S
Fujioka
S
S
Tamura
T
T
Kobatake
Y
Y
Keno
S
S
Tarui
D000199:Actins / Q000378:metabolism; D000818:Animals; D001055:Apolipoproteins B / Q000378:metabolism*; D015152:Blotting, Northern; D001835:Body Weight; D005260:Female; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014697:Ventromedial Hypothalamic Nucleus / Q000473:pathology*
D000199:Actins; D001055:Apolipoproteins B; D008079:Lipoproteins, VLDL; D012333:RNA, Messenger