Evidence for proliferation and differentiation of endosteal cells into hemopoietic cell lines in short-term liquid culture.
To study the ability of endosteal cells to spontaneously differentiate in vitro, cells isolated from the endosteal bone surface (endosteal cells) were incubated in liquid suspension cultures containing fetal calf serum and antibiotics, but without chemical inducers of differentiation. Cultures were examined daily for the appearance of morphologically recognizable mature and differentiated hemopoietic cells. Culture outcomes were uniformly consistent, showed an almost complete disappearance of the endosteal cells (which appeared as stimulated lymphoid and late stage normoblast-like cells once they are separated from the bone surfaces), and their replacement by differentiated and mature hemopoietic cells. The ability of endosteal cells to fully differentiate into mature hemopoietic cells in vitro indicates that endosteal cells represent the multipotential hemopoietic stem cells in vivo, and are equivalent of embryonal stage undifferentiated mesenchymal cells.
Department of Medical Oncology, Roswell Park Memorial Institute, Buffalo, NY 14263.
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Islam
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Glomski
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Henderson
D000818:Animals; D001854:Bone Marrow Cells; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005269:Femur / Q000166:cytology; D006410:Hematopoiesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL