Permeability and penicillin-binding protein alterations in Salmonella muenchen: stepwise resistance acquired during beta-lactam therapy.
A patient with Salmonella muenchen sepsis was unsuccessfully treated with ampicillin. During therapy, four strains that showed stepwise ampicillin resistance and affected other beta-lactams and unrelated antibiotics were isolated sequentially. Resistance was caused by decreased outer membrane permeability associated with diminished expression of porin OmpF. Furthermore, the most resistant isolate overproduced the PBP 3 target molecule.
Département de Microbiologie, Centre Médical Universitaire de Genève, Switzerland.
F
F
Bellido
I R
IR
Vladoïanu
R
R
Auckenthaler
S
S
Suter
P
P
Wacker
R L
RL
Then
J C
JC
Pechère
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D001345:Autoradiography; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D006602:Hexosyltransferases; D006801:Humans; D007223:Infant; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D010539:Permeability; D010957:Plasmids; D012475:Salmonella / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012480:Salmonella Infections / Q000188:drug therapy / Q000382:microbiology; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D046915:Penicillin-Binding Proteins; D047090:beta-Lactams; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase