Histamine methyltransferase in dispersed cells from rabbit fundic mucosa.
The cellular distribution of histamine N-methyltransferase was studied in rabbit gastric mucosa. The fundic mucosa was dispersed by collagenase treatment in Hanks' or calcium-free medium. In calcium-free medium, the number of dispersed cells/g wet tissue, as well as their viability was increased; histamine N-methyltransferase recovery was up to three-fold larger than in cells prepared in Hanks' medium. Furthermore, the calcium-free medium led to a greater acid secretory response, whereas the cellular pepsinogen content tended to be lower. Histamine N-methyltransferase activity was found in all cell fractions but was higher in the larger cell types. The enzyme activity showed only a partial correlation with either oxyntic or chief cells. These results indicate that the use of calcium-free medium to disperse and isolate rabbit mucosal cells improves cell quality. Histamine N-methyltransferase in the rabbit fundic mucosa, is found in more than one cell type, primarily the oxyntic and chief cells.
Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
J
J
Loiselle
A
A
Wollin
D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D005748:Gastric Fundus / Q000166:cytology / Q000201:enzymology; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology*; D006637:Histamine N-Methyltransferase / Q000032:analysis*; D008780:Methyltransferases / Q000032:analysis*; D010295:Parietal Cells, Gastric / Q000201:enzymology; D011817:Rabbits
D000632:Aminopyrine; D008780:Methyltransferases; D006637:Histamine N-Methyltransferase