Levels of disialogangliosides in sera of melanoma patients monitored by sensitive thin-layer chromatography and immunostaining.
Levels of GD2, GD3, and 9-O-acetyl GD3 were monitored in sera of patients with melanoma and healthy adults with two monoclonal antibodies that specifically detect these gangliosides. By direct measurement of radioactivity in the immunolabeled chromatogram, GD2 could be detected in normal sera at 2 ng/mL. Serum levels of GD2 and GD3 were increased approximately sixfold and fivefold, respectively, in patients with disseminated melanoma, compared with those of healthy adults. The acetylated derivative of GD3, which is highly specific for melanoma cells, was not detected in serum. This sensitive assay allows the quantitation of tumor-associated gangliosides that are circulating in sera of melanoma patients.
Sela
B A
BA
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Iliopoulos
D
D
Guerry
D
D
Herlyn
D
D
Koprowski
H
H
D000328:Adult; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D014408:Biomarkers, Tumor / Q000097:blood; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000097:blood*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D008545:Melanoma / Q000097:blood*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D005732:Gangliosides; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; C026226:ganglioside, GD3; C019403:ganglioside, GD2; C049132:9-O-acetyl-GD3 ganglioside