Inhibition of interferon-alpha/beta induction in L-929 cells by benzene and benzene metabolites.
Murine L-929 cells were treated with benzene or a series of benzene metabolites, washed and then interferon-alpha/beta was induced with polyriboinosinic-polyribocytidylic acid. Exposure of the cells to benzene or phenol, a monocyclic metabolite of benzene, did not affect interferon-alpha/beta induction. However, exposure of the cells to p-benzoquinone, hydroquinone or catechol, dihydroxy- and diketo-metabolites of benzene, resulted in a severe inhibition of interferon-alpha/beta production. There was no significant loss of viability of the cell cultures. Additional studies with p-benzoquinone indicated that inhibition of interferon-alpha/beta was reversible and could be abrogated by addition of reduced glutathione to the cell cultures.
Department of Microbiology and Immunology, School of Medicine, University of Louisville, Ky.
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SC
Cheung
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Nerland
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Sonnenfeld
D000818:Animals; D001554:Benzene / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology*; D016227:Benzoquinones; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000494:pharmacology; D007370:Interferon Type I / Q000096:biosynthesis*; D007739:L Cells; D051379:Mice; D019800:Phenol; D010636:Phenols / Q000494:pharmacology; D011809:Quinones / Q000494:pharmacology; D013329:Structure-Activity Relationship
D001555:Benzene Derivatives; D016227:Benzoquinones; D007370:Interferon Type I; D010636:Phenols; D011809:Quinones; D019800:Phenol; C004532:quinone; D005978:Glutathione; D001554:Benzene