Low polarity ligands of sex hormone-binding globulin in pregnancy. Part II--Identification.
Certain previously unrecognized ligands of SHBG of low polarity in pregnancy were identified. They include two weakly bound compounds: 5 alpha-pregnane-3,20-dione and progesterone; and two strongly bound substances, 2-methoxyestrone and a new steroid, estradienolone (17 beta-hydroxy-1,5-estradiene-3-one). The identification of the first three peaks was based on chromatographic elution patterns, binding characteristics and gas chromatography-mass spectrometry. The identification of the fourth peak, the new steroid, was based on similar kinds of evidence and, in addition, solubility characteristics and ultraviolet absorption spectrum.
Philip
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Reproductive Physiology Unit, Montreal General Hospital, Canada.
Murphy
B E
BE
D043582:5-alpha-Dihydroprogesterone; D002851:Chromatography, High Pressure Liquid; D004963:Estrenes / Q000032:analysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis; D006894:Hydroxyestrones / Q000032:analysis; D007745:Labor Onset; D009277:Nandrolone / Q000032:analysis; D011247:Pregnancy / Q000097:blood*; D011277:Pregnanediones / Q000032:analysis; D011374:Progesterone / Q000032:analysis; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*
D004963:Estrenes; D006894:Hydroxyestrones; D011277:Pregnanediones; D012738:Sex Hormone-Binding Globulin; D011374:Progesterone; D009277:Nandrolone; D043582:5-alpha-Dihydroprogesterone; C003655:2-methoxyestrone; D006854:Hydrocortisone