Derivatization of N-methyl and cyclic amino acids with dimethylformamide dimethyl acetal.
Six amino acids containing either an N-methyl or a cyclic secondary amine were converted to volatile derivatives by reaction with dimethylformamide dimethyl acetal. The amine functionalities were formylated by way of an amide acetal intermediate while the carboxylic acid groups were esterified directly. The resulting N-formyl esters were stable to solvent extraction and exhibited gas chromatography-mass spectrometry properties suitable for assay development.
Grubb
M F
MF
Department of Medicinal Chemistry/Pharmacognosy, University of Maryland, Baltimore 21201.
Callery
P S
PS
D000596:Amino Acids / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D004126:Dimethylformamide / Q000031:analogs & derivatives*; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents
D000596:Amino Acids; D007202:Indicators and Reagents; C022083:dimethylformamide-dimethylacetal; D004126:Dimethylformamide