Characterization of oligosaccharides by lectin affinity high-performance liquid chromatography.
Alterations of the oligosaccharide structures of glycoproteins are associated with differentiation, malignant transformation, and expression of the same protein in different cell types. The potential biological importance of oligosaccharides has resulted in a growing need for detailed structural information. When glycoproteins are available in limited quantities and/or bear highly heterogeneous oligosaccharides, characterization of their oligosaccharides is difficult. We have developed an efficient approach for obtaining detailed information about oligosaccharides by determining structural 'fingerprints' using lectin affinity high-performance liquid chromatography.
Green
E D
ED
Baenziger
J U
JU
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037102:Lectins / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis*
D037102:Lectins; D009844:Oligosaccharides