Isolation and characterization of an acidic lethal phospholipase Az from Malayan cobra (Naja naja sputatrix) venom.
An acidic, lethal phospholipase Az was purified to electrophoretic homogeneity from the venom of the Malayan cobra (Naja naja sputatrix). The enzyme has an isoelectric point of 5.58, a molecular weight of 12000, and a medium lethal dose (LD50) of 0.86 micrograms/g in mice by intravenous injection. The enzyme also exhibited weak anticoagulant and edema-forming activities. The amino acid composition of the enzyme is similar to those of other cobra venom phospholipases Az.
Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur.
N H
NH
Tan
A
A
Arunmozhiarasi
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004546:Elapid Venoms / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000302:isolation & purification* / Q000633:toxicity; D012911:Snakes
D000596:Amino Acids; D004546:Elapid Venoms; D010740:Phospholipases; D010741:Phospholipases A