Studies on the ability of smoke from different types of cigarettes to induce DNA single-strand breaks in cultured human cells.
In this paper we report preliminary studies using alkaline elution to examine the incidence of DNA-strand breakage in human lung cells exposed to smoke/phosphate-buffered saline generated from cigarettes of different tar contents and filter status. The majority of the DNA breaks induced were abolished by catalase indicating a role for active oxygen species. The incidence of breaks did not correlate with the tar content of the cigarettes. The presence of a filter in the cigarette reduced the TPM concentration of the mainstream smoke but did not reduce the number of single-strand breaks occurring in DNA after exposure to smoke/PBS. This last parameter was however reduced if the filter was ventilated.
Fielding
S
S
School of Biological Sciences, University College of Swansea, Great Britain.
Short
C
C
Davies
K
K
Wald
N
N
Bridges
B A
BA
Waters
R
R
D002374:Catalase / Q000494:pharmacology; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects; D010947:Plants, Toxic; D012906:Smoke / Q000009:adverse effects*; D013638:Tars / Q000633:toxicity; D014026:Nicotiana
D004277:DNA, Single-Stranded; D012906:Smoke; D013638:Tars; D002374:Catalase