Effect of ferric nitrilotriacetate on predominantly cortical neuronal cell cultures.
Predominately neuronal cell cultures were produced as described in previous communications. Neuronal cells were exposed to ferric nitrilotriacetate (Fe-NTA) at varying concentrations. Studies of the neuronal cells were performed at 13 and 20 days in culture. In addition to morphologic studies, biochemical assays including choline acetyltransferase (ChAT) activity, specific [3H]flunitrazepam (FLU) binding, clonazepam (CLO)-displaceable [3H]FLU binding, Ro5-4864-displaceable [3H]FLU binding, high-affinity [3H]GABA uptake, and protein determinations were performed. The data demonstrate that chelated ferric iron has an adverse effect on predominately neuronal cultures after 7 days of exposure as measured by choline acetyltransferase activity, while other measures remained unaffected; however, after 14 days of exposure all measures were significantly decreased. The effects of Fe-NTA exposure appear to be both concentration and duration-of-exposure related.
Division of Pediatric Neurology, University of Minnesota Medical School, Minneapolis 55455.
K F
KF
Swaiman
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Machen
D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D001570:Benzodiazepinones / Q000378:metabolism; D001667:Binding, Competitive; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002998:Clonazepam / Q000378:metabolism; D005290:Ferric Compounds / Q000494:pharmacology*; D005445:Flunitrazepam / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000085:Acetates; D001570:Benzodiazepinones; D005290:Ferric Compounds; D011506:Proteins; C028513:4'-chlorodiazepam; D005680:gamma-Aminobutyric Acid; D002998:Clonazepam; D005445:Flunitrazepam; D002795:Choline O-Acetyltransferase; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate