[Synthesis of 1-(difluoro-1,3,5-triazinyl)-2-methylisoindole as a fluorescent derivatization reagent and its reactivity for corticosteroids].
A fluorescence high-performance liquid chromatographic method for the determination of corticosteroids is described. Corticosteroids were labeled quantitatively by treatment with 1-(difluoro-1,3,5-triazinyl)-2-methylisoindole in an alkaline medium at room temperature for 10 s and the fluorescent adducts were eluted isocratically on a reversed-phase column by a mobile phase of aqueous methanol. The detection limit of cortisone was 3 pmol for an injection volume of 10 microliter.
H
H
Fujino
S
S
Goya
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003348:Cortisone / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007211:Indoles / Q000138:chemical synthesis*; D014227:Triazines / Q000138:chemical synthesis*
D005456:Fluorescent Dyes; D007211:Indoles; D014227:Triazines; C060535:1-(4,6-difluoro-1,3,5-triazinyl)-2-methylisoindole; D003348:Cortisone