Dot-enzyme-linked immunosorbent assay (dot-ELISA) for the rapid diagnosis of human fascioliasis.
A dot-enzyme-linked immunosorbent assay (dot-ELISA) was developed as a fast and field applicable antibody detection tool for the diagnosis of human fascioliasis. The assay is performed using partially purified antigens from a species of Fasciola at 180 ng protein/dot (2 microliters) and serum samples at 1:20 dilution (1 microliter). Dot-ELISA results completely agreed with those of micro-ELISA. Antigen-coated nitrocellulose sheets stored for 3 mo at -20 C showed results identical to fresh sheets. Sera from patients with fascioliasis (n = 30) and other parasitic or viral infections (n = 120) were compared with sera from healthy controls (n = 14). Ninety samples can be tested within 90 min. The sensitivity, specificity, and speed of the assay may justify its use in laboratory and field studies.
U.S. Naval Medical Research Unit No. 3, Cairo, Egypt, F.P.O., New York 09527.
H I
HI
Shaheen
K A
KA
Kamal
Z
Z
Farid
N
N
Mansour
F N
FN
Boctor
J N
JN
Woody
D000947:Antigens, Helminth / Q000032:analysis; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005211:Fascioliasis / Q000175:diagnosis* / Q000276:immunology; D005243:Feces / Q000469:parasitology; D006801:Humans
D000947:Antigens, Helminth