Effect of dietary fat on individual long-chain fatty acyl-CoA esters in rat liver and skeletal muscle.
The effect of dietary fat on the long-chain acyl-CoA ester profile of liver and skeletal muscle was investigated by feeding weanling rats 12%-fat diets composed of high-linoleic safflower oil (73% 18:2n-6), high-oleic safflower oil (70% 18:1n-9) or olive oil (70% 18:1n-9) for six and ten weeks. Approximately 50% of both hepatic and skeletal muscle acyl-CoA esters comprised linoleoyl-CoA or oleoyl-CoA with high-linoleic or oleic feeding, respectively. Total hepatic acyl-CoA ester concentration was 40% higher (p less than 0.05) in rats fed 12% fat compared with controls fed a 4%-fat diet. These data demonstrate that the long-chain acyl-CoA ester profile of liver and skeletal muscle reflects the dietary fatty acid profile.
Department of Nutritional Sciences, University of Wisconsin, Madison 53706.
D M
DM
Ney
J B
JB
Lasekan
T
T
Spennetta
M
M
Grahn
E
E
Shrago
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology; D008041:Linoleic Acids / Q000008:administration & dosage; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000008:administration & dosage; D051381:Rats; D011919:Rats, Inbred Strains
D000214:Acyl Coenzyme A; D004041:Dietary Fats; D005227:Fatty Acids; D008041:Linoleic Acids; D009829:Oleic Acids; D019301:Oleic Acid