Studies on the mechanism of rubidium-induced kaliuresis.
Renal clearance and electron microprobe methods were used 1) to elucidate the effects of chronic rubidium administration on potassium transport and 2) to localize, by the use of amiloride in acute experiments, the tubule site of interaction between rubidium and potassium. Substitution of drinking water by a 50 mM rubidium chloride solution for 9 to 11 days led to significant hypokalemia (plasma potassium 2.5 +/- 0.1 mM; plasma potassium plus rubidium 3.3 +/- 0.1 mM). Compared to a control group (reduction of plasma potassium to 3.4 +/- 0.1 mM by short-term potassium depletion) with a fractional potassium excretion of 2.1 +/- 0.3%, rubidium-treated rats excreted potassium at a much higher rate of 14.6 +/- 3.0%. The potassium content of principal cells was, however, significantly lower in rubidium-treated than in potassium-deprived animals. Similar to experiments in which rubidium was given acutely (3 hours), chronic rubidium administration was associated with preferential accumulation of rubidium in all tubule cells relative to potassium. Rubidium clearances were uniformly below those of potassium. Amiloride abolished the difference between rubidium and potassium clearances and sharply reduced the excretion of both cations. In view of the known site of action of amiloride, this suggests a distal tubule site of rubidium action on potassium transport. Amiloride also reduced or abolished the preferential uptake of rubidium into all but intercalated tubule cells. Marked cell heterogeneity of rubidium accumulation into intercalated cells was observed: One subpopulation, with low cell chloride, retained rubidium more effectively than another subpopulation with high cell chloride.
Department of Physiology, University of Munich, Federal Republic of Germany.
F X
FX
Beck
A
A
Dörge
G
G
Giebisch
K
K
Thurau
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D004573:Electrolytes / Q000097:blood / Q000652:urine; D005919:Glomerular Filtration Rate / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology; D008297:Male; D011188:Potassium / Q000097:blood / Q000652:urine*; D011191:Potassium Deficiency / Q000097:blood / Q000652:urine*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012413:Rubidium / Q000097:blood / Q000633:toxicity* / Q000652:urine
D004573:Electrolytes; D000584:Amiloride; D012413:Rubidium; D011188:Potassium