Zeta-crystallin, a novel protein from the guinea pig lens is related to alcohol dehydrogenases.
zeta-Crystallin is a major component of the water-soluble proteins of the guinea pig lens. We have constructed a lens cDNA library from one- to seven-day-old guinea pigs in the plasmid Bluescript KS+ and used the 16 amino acid (aa) sequence of a CNBr peptide to design an oligodeoxyribonucleotide probe. Analysis of two positive clones and direct sequence of the 5' end of the RNA resulted in the completion of a most probably full-length mRNA comprising 1842 nucleotides (nt). The ATG start codon occurs 83 nt downstream from the 5' end. The open reading frame, ending with a stop codon at nt position 1070, predicts a protein of 328 aa with a calculated Mr of 35,071. Comparison of the amino acid sequence with the National Biomedical Research Foundation protein data base reveals a significant similarity of zeta-crystallin with the enzyme of the alcohol dehydrogenase family.
Laboratory of Mechanisms of Ocular Diseases, National Eye Institute, Bethesda, MD 20892.
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A
Rodokanaki
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RK
Holmes
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Borrás
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D003459:Crystallins / Q000096:biosynthesis / Q000235:genetics*; D004247:DNA / Q000235:genetics; D001330:Electronic Data Processing; D005786:Gene Expression Regulation; D006168:Guinea Pigs; D007908:Lens, Crystalline / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*
D003062:Codon; D003459:Crystallins; D012333:RNA, Messenger; D004247:DNA; D000426:Alcohol Dehydrogenase