Determination of enantiomeric purity of tranylcypromine sulfate by proton magnetic resonance spectroscopy with chiral lanthanide shift reagent.
Optimum experimental conditions were developed for determination of the optical purity of samples of tranylcypromine sulfate by proton magnetic resonance spectroscopy after complexation with the chiral lanthanide chelate Eu(hfc)3. At a substrate concentration of 0.25M (0.125M as sulfate) in CDCl3 and an Eu(hfc)3 to substrate molar ratio of 1, the methine proton geminal to the amino group in the cyclopropane ring showed the largest induced shift and largest enantiomeric induced shift difference. From the relative intensities of the resolved (+)-CH-NH2 protons (15.77 ppm) and (-)-CH-NH2 proton (16.04 ppm), the enantiomeric purity and percentage compositions were readily calculated. The mean +/- SD recovery of (+)-tranylcypromine sulfate from synthetic enantiomeric mixtures was 101.02 +/- 2.59 (n = 6).
Hanna
G M
GM
Food and Drug Administration, New York Regional Laboratory, Brooklyn 11232.
Lau-Cam
C A
CA
D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008674:Metals, Rare Earth; D013237:Stereoisomerism; D014191:Tranylcypromine / Q000032:analysis*
D007202:Indicators and Reagents; D008674:Metals, Rare Earth; D014191:Tranylcypromine