Nonlinearity in protein assays by the Coomassie blue dye-binding method.
The Coomassie brilliant blue (CBB) method for protein determination takes advantage of the fact that a low-pH red form of CBB reverts to a blue form when CBB binds to protein. The increase in absorbance at 595 nm of a protein-dye mixture compared to a blank containing only the dye reagent has been used to estimate the total amount of protein present in the sample mixture. A disadvantage of this method of protein determination is that the assay plot of absorbance at 595 nm versus total protein is not linear.
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Gustavus Adolphus College, St. Peter, Minnesota 56082.
Sohl
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D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes; D013056:Spectrophotometry, Ultraviolet
D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue