Determination of arteether in blood plasma by high-performance liquid chromatography with ultraviolet detection after hydrolysis acid.
A reversed-phase high-performance liquid chromatographic method is described for determination of the antimalarial agent arteether in blood plasma based on its decomposition in acidic medium and measurement of the major decomposition product, which has been identified as an alpha,beta-unsaturated decalone. Linear calibration curves were obtained in the range 0-250 ng/ml arteether and the recovery of the drug from plasma was found to be quantitative. There is no interference from desoxyarteether, the putative major metabolite of arteether. The method has been applied to the measurement of arteether in the plasma of rats given 110 mg/kg by intramuscular injection of the drug as a solution in sunflower oil.
Department of Pharmacolgy and Therapeutics, University of Liverpool, UK.
O R
OR
Idowu
G
G
Edwards
S A
SA
Ward
M L
ML
Orme
A M
AM
Breckenridge
D000818:Animals; D000962:Antimalarials / Q000097:blood*; D037621:Artemisinins; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006851:Hydrochloric Acid; D006868:Hydrolysis; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D000962:Antimalarials; D037621:Artemisinins; D007202:Indicators and Reagents; D012717:Sesquiterpenes; D006851:Hydrochloric Acid; C055141:artemotil