Determination of halofuginone in bovine plasma by competing-ion high performance liquid chromatography after solid phase extraction.
A high performance liquid chromatography (HPLC) method for the determination of the anticoccidial and antitheilerial drug halofuginone in bovine plasma was developed. Samples were diluted with acetic acid (10%, v/v) and cleaned up on a Bond Elut C8 column. The analyte was eluted from the extraction column and chromatographed by reversed-phase HPLC using decylamine as a competing-ion reagent. Detection was by UV at 243 nm. Recovery from plasma was 75%, and within-day and between-day coefficients of variation were 5.23 and 6.35% respectively. The specificity and sensitivity of this method (limit of detection in plasma, 1 ng/mL) were sufficiently high to enable us to characterize the time course of the drug in plasma after oral administration of therapeutic doses to cattle.
Kinabo
L D
LD
Department of Veterinary Pharmacology, University of Glasgow Veterinary School, UK.
McKellar
Q A
QA
Murray
M
M
D000284:Administration, Oral; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003049:Coccidiostats / Q000097:blood*; D007202:Indicators and Reagents; D008832:Microchemistry; D010880:Piperidines; D011799:Quinazolines / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification; D052999:Quinazolinones
D003049:Coccidiostats; D007202:Indicators and Reagents; D010880:Piperidines; D011799:Quinazolines; D052999:Quinazolinones; C010176:halofuginone