High-molecular-mass ("biliary") isoenzyme of alkaline phosphatase and the diagnosis of liver dysfunction in cystic fibrosis.
The high-Mr isoenzyme of alkaline phosphatase (AP, EC 3.1.3.1), a highly sensitive index to cholestasis, was measured by liquid chromatography in 45 patients with cystic fibrosis. Results of serum tests for liver dysfunction--including gamma-glutamyltransferase, aspartate aminotransferase, alanine aminotransferase, total AP, bilirubin, and bile acids--were compared with those for high-Mr AP. Values for high-Mr AP were increased in 44.4% of our patient population, with activities ranging from 0.4 to 17.3 U/L. The upper limit in the control group was 2.5 U/L. We find increased high-Mr AP to be a more sensitive indicator of liver dysfunction in patients with cystic fibrosis than are other tests.
Klinik mit Poliklinik für Kinder und Jugendliche der Friedrich-Alexander Universität Erlangen-Nürnberg, F.R.G.
E
E
Schoenau
W
W
Boeswald
R
R
Wanner
K H
KH
Herzog
B
B
Boewing
H J
HJ
Boehles
K
K
Stehr
D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood*; D001659:Biliary Tract / Q000201:enzymology*; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000150:complications*; D005260:Female; D006801:Humans; D007223:Infant; D007527:Isoenzymes / Q000097:blood*; D008107:Liver Diseases / Q000150:complications / Q000201:enzymology*; D008111:Liver Function Tests; D008297:Male; D008970:Molecular Weight
D007527:Isoenzymes; D000469:Alkaline Phosphatase