Binding of myosin I to membrane lipids.
The single-headed myosins called myosin-I were first isolated from the protozoan Acanthamoeba and subsequently identified in other cells. We previously reported evidence that myosin-I is responsible for the movement of membranes, extracted from Acanthamoeba, along actin filaments in vitro. Here we show for the first time that myosin-I can bind directly to NaOH-extracted membranes isolated from Acanthamoeba and to vesicles of pure lipids with an affinity sufficient for extensive binding in the cell. Membrane-bound myosin-I may provide a mechanism for many cellular movements previously thought to involve filamentous myosin-II.
Department of Cell Biology and Anatomy, Johns Hopkins Medical School, Baltimore, Maryland 21205.
R J
RJ
Adams
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TD
Pollard
D000048:Acanthamoeba / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002465:Cell Movement; D008563:Membrane Lipids / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*
D008563:Membrane Lipids; D009218:Myosins