[Anoxia-reoxygenation injury in isolated rat myocytes is modulated by cell sodium during the anoxia period].
Using the model of anoxia-reoxygenation injury in isolated rat myocytes, we observed that incubation of myocytes with ouabain, inhibitor of Na+-K+ ATPase, during anoxia period significantly increased the cell sodium content. These myocytes demonstrated severe injury and intracellular calcium overload during reoxygenation period. The sodium content of myocytes at the end of anoxia period was positively correlated with the overload of intracellular calcium at reoxygenation (r = 0.882, P less than 0.01). Mn2+, an inhibitor of Na+-Ca2+ exchange, significantly attenuated the anoxia-reoxygenation injury when given during reoxygenation period. Mn2+ also inhibited the cell injury caused by incubation of myocytes with Na+-free medium. These results suggest that anoxia-reoxygenation injury of rat myocytes is modulated by cell sodium during the anoxia period, and the Na+-Ca2+ exchange mechanism plays an important role in influx of extracellular calcium during reoxygenation period.
Z P
ZP
Li
C S
CS
Tang
J Y
JY
Su
D000818:Animals; D002118:Calcium / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008345:Manganese / Q000494:pharmacology; D015428:Myocardial Reperfusion Injury; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism*
D008345:Manganese; D010042:Ouabain; D012964:Sodium; D010100:Oxygen; D002118:Calcium