Bindings of toxic shock syndrome toxin-1 and staphylococcal enterotoxins A, B, and C to rabbit spleen cells.
Toxic shock syndrome toxin-1 (TSST-1) and staphylococcal enterotoxins (SE) A, B, and C were studied on binding to rabbit spleen cells. The toxins showed remarkable mitogenic effects on the cells. Among them, SEA and TSST-1 had much stronger mitogenic activities than SEB and SEC. Binding study showed that labeled TSST-1 and SEA bound considerably to cells, but that labeled SEB or SEC was not observed to bind at a detectable level under the same conditions as TSST-1 and SEA. Competitive binding analysis between toxins to cells proved that TSST-1 and SEA clearly competed with each other in binding. Scatchard plots for TSST-1 and SEA in binding were linear at the doses used. The Scatchard analysis for TSST-1 and SEA gave a dissociation constant of 2.5 X 10(-9) M and 7.6 X 10(-8) M and the number of binding sites per cell of 5.3 X 10(3) and 1.0 X 10(5), respectively.
Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health.
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Fujikawa
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Takayama
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Uchiyama
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Igarashi
D000818:Animals; D001427:Bacterial Toxins; D001667:Binding, Competitive; D004768:Enterotoxins / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D008934:Mitogens; D011817:Rabbits; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013211:Staphylococcus aureus; D018089:Superantigens
D001427:Bacterial Toxins; D004768:Enterotoxins; D008934:Mitogens; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal; C034959:enterotoxin C, staphylococcal