The organ distribution of liposome-encapsulated and free cobalt in rats. Liposomes decrease the cardiac uptake of the metal.
Rats were administered intravenously liposome-encapsulated or free cobalt, and the organ distribution of the metal was explored using Co57 tracer. Two hours after administration, the cobalt level in the heart was about 40% of the control when given in sphingomyelin (SM)/cholesterol (CH) (1:1 mole ratio) liposomes. These vesicles also tended to decrease the uptake of cobalt in the kidney and the carcass, and to increase it in the spleen and the bones. Liposomes prepared from soybean phosphatidylcholine (SPC)/CH (1:1) had no effect on the uptake of cobalt in the heart, whereas increased its level in the spleen, liver and lung. The time-course of cobalt deposition in the organs displayed substantial variation with the different preparations. Most importantly, no buildup of cobalt level was observed in the heart when the metal was administered in SM/CH vesicles. While confirming known effects of liposomes on the organ-distribution of entrapped drugs, our findings suggest that administration of cobalt in SM/CH liposome-encapsulated form may result in decreased cardiotoxicity and thus increased safety of cobalt-treatment in some anemias.
Dept. Surgical Biology, University of Arizona, Tucson 85724.
J
J
Szebeni
R
R
Garcia
C D
CD
Eskelson
M
M
Chvapil
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002642:Chick Embryo; D003035:Cobalt / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D004337:Drug Carriers; D007668:Kidney / Q000378:metabolism; D008081:Liposomes; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010713:Phosphatidylcholines; D051381:Rats; D011919:Rats, Inbred Strains; D013025:Glycine max; D013109:Sphingomyelins; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
D004337:Drug Carriers; D008081:Liposomes; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D003035:Cobalt