Antigenic sites on ribonuclease A. Synthetic peptides corresponding to the sites 37-42 and 83-88 on ribonuclease A show immunogenicity.
Two hexapeptides NH2-Lys-Asp-Arg-Cys-Lys-Pro-COOH and NH2-Asp-Cys-Arg-Glu-Thr-Gly-COOH corresponding to the strong hydrophilic regions 37-42 and 83-88, respectively, on ribonuclease A were synthesized by solid-phase method. These synthetic peptides showed antigenic characteristics and provided an experimental validity to the prediction made earlier, supporting the view that highly hydrophilic regions on the protein have a good correlation with their being potentially antigenic.
Centre for Cellular and Molecular Biology, Hyderabad, India.
A J
AJ
Sagar
Y V
YV
Mahalakshmi
R
R
Nagaraj
M W
MW
Pandit
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D001665:Binding Sites; D003429:Cross Reactions; D003488:Cyanogen Bromide; D007120:Immunochemistry; D010455:Peptides / Q000138:chemical synthesis / Q000302:isolation & purification; D011489:Protein Denaturation; D011817:Rabbits; D012259:Ribonuclease, Pancreatic / Q000276:immunology*; D012709:Serum Albumin / Q000378:metabolism
D000906:Antibodies; D000941:Antigens; D010455:Peptides; D012709:Serum Albumin; D012259:Ribonuclease, Pancreatic; D003488:Cyanogen Bromide