Development and testing of a synthetic oligonucleotide probe for the detection of pathogenic Yersinia strains.
A 24-base oligonucleotide probe specific for a region of the Yersinia enterocolitica virulence plasmid (pYV) associated with HEp-2 cell cytotoxicity and the Sereny reaction was constructed by using sequences flanking critical TnphoA insertions in a subcloned fragment of pYV. This probe, highly specific and sensitive for virulent yersiniae, detected pathogenic Y. enterocolitica isolates in artificially inoculated foods.
Miliotis
M D
MD
Department of Medicine, University of Maryland School of Medicine, Baltimore, 21201.
Galen
J E
JE
Kaper
J B
JB
Morris
J G
JG
D001483:Base Sequence; D002460:Cell Line; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D005516:Food Microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011237:Predictive Value of Tests; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
D015342:DNA Probes; D004269:DNA, Bacterial