Acetate inhibition of chick bone cell proliferation and bone growth in vitro.
A hypothesis has been advanced that parenteral solutions as commonly formulated for use in clinical practice have a toxic effect on cell metabolism. A specific component of these solutions, sodium acetate, has been suggested to disrupt normal bone turnover and therefore to contribute to the osteopenia observed in patients receiving hemodialysis and parenteral nutrition (PN). We developed an in vitro model to test the hypothesis that sodium acetate at concentrations that are infused in PN solutions has a deleterious effect on bone metabolism. Osteoblasts and preosteoblasts from 16- to 17-day-old embryonic chick calvaria, and tibiae and femora from 10-day-old embryonic chicks were grown in BGJb medium (control) or in BGJb medium plus sodium acetate (5, 10, or 20 mM). Calvarial cell proliferation was quantified by direct cell counts as well as by incorporation of [3H]TdR into DNA as an index of cell proliferation. Calvarial cell alkaline phosphatase activity was quantified by the ability of extracts of the cultured cells to hydrolyze p-nitrophenyl phosphate to p-nitrophenol, and bone growth was determined by measuring final dry weight. Calvarial cell counts as well as DNA synthesis showed a dose-dependent decrease in the presence of sodium acetate (5-20 mM) compared with controls. [3H]TdR incorporation was decreased a mean 19% with 5 mM, 38% with 10 mM, and 63% with 20 mM acetate. Alkaline phosphatase activity per cell increased 48% with 5 mM, 140% with 10 mM, and 355% with 20 mM acetate. Cell viability as assessed by trypan blue exclusion was identical for test and control media (greater than 95%).(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Medicine, University of Washington, Seattle 98195.
J C
JC
Saitta
E W
EW
Lipkin
G A
GA
Howard
D000085:Acetates / Q000494:pharmacology*; D019342:Acetic Acid; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001790:Blood Physiological Phenomena; D001846:Bone Development / Q000187:drug effects*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D009924:Organ Culture Techniques; D009929:Organ Size / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects
D000085:Acetates; D000469:Alkaline Phosphatase; D019342:Acetic Acid