Inhibitory effect of tannin on poly(ADP-ribose) glycohydrolase from human placenta.
Tannin, a naturally occurring polyphenolic compound, was found to inhibit the activity of purified poly(ADP-ribose) glycohydrolase from human placenta. The inhibition was dose-dependent and half maximal with 2.8 micrograms/ml of tannin. The inhibitory effect of tannin was two and three orders of magnitude more than those of ADP-ribose and cAMP, respectively. Kinetic analysis revealed that the inhibition by tannin was competitive with respect to the substrate poly(ADP-ribose).
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
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Tanuma
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Sakagami
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D001665:Binding Sites; D001667:Binding, Competitive; D005260:Female; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors*; D006801:Humans; D007700:Kinetics; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013634:Tannins / Q000494:pharmacology*
D013634:Tannins; D006026:Glycoside Hydrolases; C022407:poly ADP-ribose glycohydrolase