High-performance liquid chromatography of amino acids, peptides, proteins and polynucleotides. XCIV. Solid-phase hybridization of complementary oligonucleotides.
The ability of synthetic oligonucleotides to specifically hybridize to a complementary oligonucleotide immobilized on an anionic stationary phase has been investigated. A sigmoidal melting curve was obtained when oligonucleotide duplex formation on the column was plotted against hybridization stringency over the ionic strength range 0.2-0.42 M. These studies confirm a rapid method for determining the relative melting temperature of hybridized oligonucleotide complexes and provide a basis for the selection of stringency conditions optimal for various synthetic oligonucleotide probes.
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
M
M
Guthridge
J
J
Bertolini
M T
MT
Hearn
D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D009841:Oligonucleotides; D010455:Peptides; D011506:Proteins