Determination of beta-carboline derivatives in biological samples by high-performance liquid chromatography with fluorescence detection.
An assay procedure for measuring plasma, urine, and feces levels of seven beta-carbolines and possible metabolites is described. The drugs are extracted with diethylether or, alternatively, with a commercially available automated extraction device after adding an appropriate internal standard. Separation from matrix constituents is performed by reversed-phase high-performance liquid chromatography (HPLC) followed by fluorescence detection. The carboxylic acid metabolites of the beta-carbolines have to be esterified before HPLC measurement. The detection limits are 0.2 ng/mL for beta-carbolines and 2 ng/mL for their metabolites. Plasma levels and pharmacokinetic parameters of six different beta-carbolines in healthy male volunteers following iv or po treatment are described. Total clearance ranged from 17 to 52 mL/min/kg and the terminal half-life ranged from 1 to 4 h. The absolute bioavailability exhibited a range from less than 1 to 61%.
Schering AG, Berlin, W. Germany.
W
W
Krause
H
H
Mengel
L
L
Nordholm
D000284:Administration, Oral; D000328:Adult; D002243:Carbolines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002264:Carboxylic Acids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000032:analysis; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D013050:Spectrometry, Fluorescence
D002243:Carbolines; D002264:Carboxylic Acids