Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50.
The plasmid pSS50 is a 53-kilobase self-transmissible plasmid of broad host range that has been isolated from several Alcaligenes and Acinetobacter species. This plasmid has previously been shown to mediate the mineralization of 4-chlorobiphenyl to carbon dioxide and water. Physical characterization of this plasmid by restriction analysis indicates that most hexanucleotide cleavage sites are clustered in a 5-kilobase region, leaving large regions without restriction sites. The paucity of restriction sites is not due to DNA methylation.
Hooper
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Department of Microbiology, University of Tennessee, Knoxville 37932-2567.
Dockendorff
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TC
Sayler
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GS
D000150:Acinetobacter / Q000235:genetics* / Q000378:metabolism; D000421:Alcaligenes / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis*; D010957:Plasmids; D015183:Restriction Mapping
D001713:Biphenyl Compounds; D004269:DNA, Bacterial; C006983:4-chlorobiphenyl