Effects of cadmium treatment in vitro on the uptake and release of [3H]serotonin by human blood platelets.
Effects of cadmium treatment on human platelets were studied with respect to uptake and release of 5-[3H]hydroxytryptamine (5-HT). The uptake of 5-[3H]HT in the presence of varying concentrations of CdCl2 (0.001-10 mM) was inhibited significantly with respect to control platelets and the inhibition was maximum at 1 mM CdCl2 concentration. From studies on the kinetics of 5-[3H]HT uptake a higher Km and significantly lower Vmax for CdCl2-treated platelets were observed. CdCl2 stimulated spontaneous release but inhibited thrombin-induced release of 5-[3H]HT. Spontaneous release of 5-[3H]HT induced by CdCl2 was not significantly altered in the presence of externally available CaCl2 (1 mM).
Mukhopadhyay
S
S
Mukhopadhyay
S
S
Addya
S
S
Bhattacharya
D K
DK
Chatterjee
G C
GC
D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D012701:Serotonin / Q000097:blood*; D013917:Thrombin / Q000494:pharmacology
D002104:Cadmium; D012701:Serotonin; D013917:Thrombin