Spectral editing techniques for 31P NMR spectroscopy of blood.
Although the measurement of the pH of biological systems using the 31P NMR response of inorganic phosphate is well known, sometimes in blood absolute determination of the intensity and position of the Pi resonance is precluded by overlap from the doublet resonance of 2,3-diphosphoglycerate. Three techniques which overcome this problem by eliminating the doublet are described and their sensitivity to the experimental parameters is assessed.
Clinical Oncology and Radiotherapeutics Unit, Medical Research Council, Cambridge, United Kingdom.
R
R
Jayasundar
T J
TJ
Norwood
L D
LD
Hall
N M
NM
Bleehen
D001774:Blood Chemical Analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010710:Phosphates
D010710:Phosphates