Direct and energy-transfer photolabelling of brain muscarinic acetylcholine receptors.
Efficient photolabelling of muscarinic acetylcholine receptors was obtained using either two aryldiazonium salts or an azido derivative. These probes did not discriminate between muscarinic binding subtypes or affinity states and became irreversibly bound to the receptor sites, in an entirely atropine-protectable manner, upon ultraviolet irradiation. The extent of labelling was dependent both on probe concentration and on time of irradiation and reached up to 80% of the receptor population, under optimal alkylating conditions. In contrast to the azido derivative, both diazonium salts behave as potent irreversible labels of muscarinic receptors, provided energy-transfer photolabelling conditions were followed. Such an indirect activation of diazonium ligands, through an energy transfer from photoexcited tryptophan residues, has been previously found to increase the site-specificity and the rate of labelling of other acetylcholine binding proteins. Analogies in the photolabelling process of acetylcholinesterase or of nicotinic and muscarinic receptors by the two diazonium salts are discussed. Altogether, these findings suggest that these new probes may be promising tools to investigate the location and the topography of the agonist-antagonist binding domain on purified muscarinic receptors, through amino acid and/or sequence analyses of radioactive, photolabelled residues.
Ilien
B
B
Laboratoire de Chimie Bio-Organique, Université Louis Pasteur, Faculté de Pharmacie, Illirch, France.
Hirth
C
C
D000345:Affinity Labels / Q000378:metabolism*; D000478:Alkylation; D000818:Animals; D001386:Azides / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D003979:Diazonium Compounds / Q000378:metabolism*; D004735:Energy Transfer; D008297:Male; D010095:Oxotremorine / Q000031:analogs & derivatives / Q000378:metabolism; D010777:Photochemistry; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*; D013053:Spectrophotometry
D000345:Affinity Labels; D001386:Azides; D003979:Diazonium Compounds; D011976:Receptors, Muscarinic; D010095:Oxotremorine; C042743:oxotremorine M; D011813:Quinuclidinyl Benzilate