Radioimmunoassay for non-enzymatically glycated serum proteins.
We have developed a radioimmunoassay (RIA) for nonenzymatically glycated serum proteins. The polyclonal antibodies prepared against reduced glycated human albumin were specific for the glucitollysine residues of serum proteins. Serum proteins from diabetic patients (n = 25) contained 5.3 +/- 2.8 nmoles of glucitollysine/mg protein, compared to 2.0 +/- 0.2 in controls (n = 20). The intra- and inter-assay variables were 3.2-6.2% and 4.4-8.6%, respectively. Results from this assay procedure correlated well with those from the boronate affinity chromatography procedure (r = 0.94; P less than 0.001). The data suggested that diabetic serum proteins contained at least 2.5 times as much immunochemically detectable glucitollysine residures as normal serum proteins after reduction of the proteins with sodium borohydride.
Kato
M
M
2nd Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Nakayama
H
H
Makita
Z
Z
Aoki
S
S
Kuroda
Y
Y
Misawa
K
K
Yoshida
H
H
Yanagisawa
K
K
Nakagawa
S
S
D000328:Adult; D000368:Aged; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002846:Chromatography, Affinity; D003920:Diabetes Mellitus / Q000097:blood; D017127:Glycation End Products, Advanced; D006023:Glycoproteins; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods; D012709:Serum Albumin / Q000032:analysis; D000093422:Glycated Serum Proteins; D000093423:Glycated Serum Albumin
D001798:Blood Proteins; D017127:Glycation End Products, Advanced; D006023:Glycoproteins; D007106:Immune Sera; D012709:Serum Albumin; D000093422:Glycated Serum Proteins; D000093423:Glycated Serum Albumin