Studies of the cation binding properties of an oligomeric derivative of prostaglandin B1.
The ionophoretic activity of PGBx, an oligomeric mixture synthesized from 15-dehydro PGB1, with different cations was measured using arsenazo III-entrapped liposomes. The order of ionophoretic activity was Zn2+ greater than Co2+ greater than Mn2+ greater than Cu2+ greater than Ca2+ greater than Ba2+ greater than Sr2+ greater than Mg2+. The intrinsic fluorescence of PGBx was quenched by the binding of divalent cations as well as by La3+ and H+. Quenching by K+ and Na+ was minimal. The order of quenching strength of divalent cations was Zn2+ greater than Co2+ greater than Cu2+ = Mn2+ greater than Ca2+ greater than Ba2+ greater than Sr2+ greater than Mg2+. Binding affinities of these cations determined by a murexide indicator method were in good agreement with that determined by the fluorescence quenching reaction. The cation binding affinity of PGBx in aqueous solutions correlates with the ionophoretic activity in liposomes. The binding affinity for K+ was estimated from the inhibition by K+ of Ca2+ binding by PGBx. Although PGBx has a lower selectivity for divalent cation binding than the ionophore A23187, the characteristics of the binding affinity of these two compounds for various ions were similar. The pK of PGBx as determined by fluorescence quenching was 6.7. The molecular weight of the divalent cation binding unit was estimated to be about 680, with each PGBx molecule having three such binding sites. The binding of Ca2+ to such a site is one-to-one.
Department of Biological Chemistry, Hahnemann University, School of Medicine, Philadelphia, PA.
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Devlin
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Ohnishi
D000818:Animals; D002413:Cations, Divalent / Q000378:metabolism*; D007202:Indicators and Reagents; D007476:Ionophores; D007700:Kinetics; D008081:Liposomes; D009282:Naphthalenesulfonates; D011108:Polymers / Q000494:pharmacology*; D011453:Prostaglandins / Q000494:pharmacology*; D011456:Prostaglandins B / Q000494:pharmacology*; D011465:Prostaglandins, Synthetic / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D002413:Cations, Divalent; D007202:Indicators and Reagents; D007476:Ionophores; D008081:Liposomes; D009282:Naphthalenesulfonates; D011108:Polymers; D011453:Prostaglandins; D011456:Prostaglandins B; D011465:Prostaglandins, Synthetic; C056888:palladiazo; C024303:prostaglandin Bx