Actin in Mucor rouxii.
Rhodamine-conjugated phalloidin was used to analyze the actin distribution during hyphal formation in Mucor rouxii. The occurrence of actin patches in the cortical region of the cells was seen in the initial stages of growth. A fungal 43 kDa protein was isolated by affinity chromatography on DNase I-sepharose. This peptide was identified on immunoblots when polyclonal antibodies against rabbit muscle actin were used as a probe. These results indicate: (1) that changes in actin localization accompany the hyphal development and (2) the fungal 43 kDa protein shares properties that are common to muscle actin.
Sabanero
M
M
Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Mexico.
Zazueta
R
R
D000199:Actins / Q000378:metabolism*; D002846:Chromatography, Affinity; D003850:Deoxyribonuclease I; D005656:Fungal Proteins / Q000378:metabolism*; D009089:Mucor / Q000254:growth & development / Q000378:metabolism*; D013172:Spores, Fungal / Q000254:growth & development
D000199:Actins; D005656:Fungal Proteins; D003850:Deoxyribonuclease I