Test-strip method for measuring lactate in whole blood.
We have developed a dry reagent strip system for measuring lactate in whole blood. The test strip contains lactate oxidase (no EC number assigned), horseradish peroxidase (EC 1.11.1.7), and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine. The system is designed to measure with a reflectometer the color that developed in the test strip, although the lactate concentration can be estimated without the reflectometer. The between-run coefficients of variation for controls at three concentrations were 2.9-5.3%. The lactate concentrations in blood samples from healthy subjects before and after exercise correlated well (r = 0.97) with the results measured by the comparison method with the use of lactate oxidase. This dry reagent strip system provides a convenient and rapid test for measuring blood lactate in clinical and sports medicine.
Shimojo
N
N
Department of Laboratory Medicine, Osaka City University Medical School, Japan.
Naka
K
K
Nakajima
C
C
Yoshikawa
C
C
Okuda
K
K
Okada
K
K
D006735:Horseradish Peroxidase; D006801:Humans; D007773:Lactates / Q000097:blood*; D006899:Mixed Function Oxygenases; D011934:Reagent Strips; D013177:Sports; D013223:Statistics as Topic; D014052:Toluidines
D007773:Lactates; D011934:Reagent Strips; D014052:Toluidines; C046221:N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-toluidine; D006899:Mixed Function Oxygenases; D006735:Horseradish Peroxidase; C021497:lactate 2-monooxygenase