Molecular cloning and nucleotide sequence of the genome of hog cholera virus.
A cDNA clone derived from genomic RNA of hog cholera virus (HCV) was identified using an oligonucleotide complementary to the RNA encoding a hexapeptide from the putative RNA-dependent RNA polymerase of the closely related bovine viral diarrhea virus (BVDV). This clone served as a probe for screening different size-selected cDNA libraries. After molecular cloning and nucleotide sequencing the HCV genome was shown to consist of 12,284 nucleotides containing one long open reading frame. Sequence comparison revealed a high degree of homology between HCV and BVDV genomic RNAs. With respect to HCV the genome of BVDV contains an insertion coding for 90 amino acids.
Meyers
G
G
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Rümenapf
T
T
Thiel
H J
HJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D006692:Classical Swine Fever Virus / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping
D012367:RNA, Viral; D004247:DNA; D012321:DNA-Directed RNA Polymerases