Tissue-specific differential modulation of arginase and ornithine aminotransferase by hydrocortisone during various developmental stages of the rat.
The activities and regulatory patterns of arginase and ornithine aminotransferase (OAT) of the liver (a mitotic tissue) and kidney cortex (a post-mitotic tissue) of immature, adult, and senescent male rats were studied. The activities of the liver enzymes were highest in the immature rat and decreased gradually with age. However, in the kidney cortex, the activity of arginase was highest and decreased significantly thereafter while that of OAT shows no significant change throughout the life span of the rat. Further, the activity of kidney cortex arginase was approximately 1/20th of that of the liver enzyme. Adrenalectomy and hydrocortisone treatments altered the activity of arginase in both tissues and that of OAT in the liver only. However, the kidney cortex OAT was not responsive towards these treatments. Actinomycin D inhibited the hydrocortisone-mediated induction of arginase of both the liver and kidney cortex and that of the liver OAT.
Department of Pharmaceutics, Banaras Hindu University, Varanasi, India.
S K
SK
Patnaik
R
R
Patnaik
D000315:Adrenalectomy; D000375:Aging; D000818:Animals; D001119:Arginase / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D006854:Hydrocortisone / Q000494:pharmacology*; D007672:Kidney Cortex / Q000201:enzymology / Q000254:growth & development*; D008099:Liver / Q000201:enzymology / Q000254:growth & development*; D008297:Male; D009928:Organ Specificity; D009953:Ornithine-Oxo-Acid Transaminase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000637:Transaminases / Q000378:metabolism*
D003609:Dactinomycin; D000637:Transaminases; D009953:Ornithine-Oxo-Acid Transaminase; D001119:Arginase; D006854:Hydrocortisone