High-resolution analysis of a hypervariable region in the human apolipoprotein B gene.
A hypervariable region occurs immediately 3' of the human apolipoprotein B gene. Several allelic variants of this tandemly repeated sequence can be resolved by genomic blotting. Higher resolution among size variants may be obtained by polymerase-chain-reaction amplification of this region followed by electrophoresis in a denaturing acrylamide gel. Fourteen different alleles containing 25-52 repeats of the basic 15-bp unit were distinguished in a population study of 318 unrelated individuals. This approach should be applicable to pedigree and linkage analysis with the apolipoprotein B gene or other tandemly repeated sequence elements.
Ludwig
E H
EH
Gladstone Foundation Laboratories for Cardiovascular Disease, University of California, San Francisco 94140-0608.
Friedl
W
W
McCarthy
B J
BJ
D000483:Alleles; D001055:Apolipoproteins B / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005784:Gene Amplification; D005787:Gene Frequency; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid
D001055:Apolipoproteins B; D015345:Oligonucleotide Probes