18, 19-Dihydroxycorticosterone: a new metabolite in human urine.
Urines of patients with primary aldosteronism were extracted, the extract repeatedly chromatographed with reversed phase HPLC. The fractions immunoactive against 18-hydroxycorticosterone antiserum and being more polar than the 18-hydroxycorticosterone were further purified, derivatized and investigated by GC/MS. In this manner the natural occurrence of the 18, 19-dihydroxycorticosterone, which was lately synthetized, in human urine was confirmed.
Department of Pharmacology, University of Heidelberg, W. Germany.
J
J
Godzsa
P
P
Vecsei
T
T
Iwuanyanwu
M
M
Harnik
D015069:18-Hydroxycorticosterone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003345:Corticosterone / Q000031:analogs & derivatives*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006929:Hyperaldosteronism / Q000652:urine*; D007202:Indicators and Reagents
D007202:Indicators and Reagents; C051957:18,19-dihydroxycorticosterone; D015069:18-Hydroxycorticosterone; D003345:Corticosterone