Immunophenotypic similarities of mediastinal clear-cell lymphoma and sinusoidal (monocytoid) B cells.
Using 14 well-defined (clustered) monoclonal antibodies (MAbs) against B-cell restricted/associated differentiation and activation (CD) antigens and 12 mediastinal clear-cell lymphomas (MCCL), 46 follicular-center-cell lymphomas (FCCL), and 20 non-neoplastic lymph nodes--including toxoplasmic and HIV-associated lymphadenitis--were immunohistochemically examined to determine the histogenesis of MCCL. Antigenically, MCCL was characterized as CD5-, CD10-, CD19+, CD20+, CD21-, CD22+, CD30-, CD37+, CDw40+, and by a frequent expression of CD11c and CD23, while other antigens were inconsistently expressed. The antigenic profiles of MCCL and FCCL showed statistically significant differences in 4/14 distinct antigens. When the neoplastic cells of both tumor groups were compared with morphologically defined normal B-cell types, the overall resemblance of their immunophenotypes was even closer between MCCL and sinusoidal (monocytoid) B cells than between FCCL and follicular-center B cells. We conclude that MCCL is a lymphoma type distinct from FCCL, most probably representing a highly malignant neoplasm corresponding to sinusoidal B-cell reaction.
Institute of Pathology, University of Heidelberg, FRG.
P
P
Möller
D U
DU
Matthaei-Maurer
W J
WJ
Hofmann
B
B
Dörken
G
G
Moldenhauer
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology; D008223:Lymphoma / Q000276:immunology*; D008479:Mediastinal Neoplasms / Q000276:immunology*; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte