Correlation of MDR1 gene expression with chemotherapy in neuroblastoma.
Forty-one neuroblastoma tumor specimens have been analyzed by Northern and slot blot hybridization techniques with human MDR1 gene probes. Only one of 15 (6%) tumors from patients who had not received chemotherapy exhibited high levels of MDR1 transcripts, while 11 of 26 (42%) treated tumors showed high levels of MDR1 expression (Fisher exact test: P = .03). The results indicate that the level of MDR1 mRNA expression is associated with previous chemotherapy, including drugs that select the multidrug resistance phenotype in vitro regardless of neuroblastoma tissue origin or N-myc content in the genome. For the 26 treated neuroblastomas, the number of nonresponsive tumors was found to be significantly higher among those with high levels of MDR1 mRNA.
Laboratoire de Pharmacologie Clinique et Moléculaire, Institut Gustave Roussy, Villejuif, France.
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J
Bourhis
J
J
Bénard
O
O
Hartmann
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L
Boccon-Gibod
J
J
Lemerle
G
G
Riou
D000310:Adrenal Gland Neoplasms / Q000235:genetics / Q000628:therapy; D000970:Antineoplastic Agents / Q000627:therapeutic use; D015152:Blotting, Northern; D001853:Bone Marrow / Q000276:immunology; D003131:Combined Modality Therapy; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics* / Q000556:secondary / Q000628:therapy; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis*; D014157:Transcription Factors / Q000032:analysis*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D015342:DNA Probes; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014157:Transcription Factors