Phenytoin induces interleukin-1 production in vitro.
Human adherent mononuclear cells and subcloned cell lines established from the human histiocytic cell line U-937 were cultured with phenytoin (PHT) and/or lipopolysaccharide (LPS) purified from Bacteroides fragilis. After the cultivation period, the cell-free supernatants were tested for interleukin-1 (Il-1) activity. The results showed that PHT induces Il-1 activity and potentiates LPS-induced Il-1 production. In the monocytic cell line U-937, the induced Il-1 production was found to be clonally distributed indicating that the response to PHT may be exerted by a subpopulation of monocytes. The PHT-induced Il-1 activity may be of importance in the development of gingival overgrowth. The induced Il-1 could also contribute to other known side effects such as dermatologic reactions accompanied by transient fever seen in patients medicating the drug.
Department of Pedodontics, Odontological Clinics, Huddinge, Sweden.
T
T
Modéer
J
J
Karsten
A
A
Weintraub
M
M
Gidlund
K G
KG
Sundqvist
D000328:Adult; D000818:Animals; D001441:Bacteroides fragilis; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D010672:Phenytoin / Q000494:pharmacology*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D010672:Phenytoin