High-performance liquid chromatographic determination of tauro- and glyco-conjugated bile acids in human serum.
A method for the identification and individual determination of the ten tauro- and glyco-conjugated bile acids is described. It consists in a specific three-step extraction from small serum samples (500 microliters), high-performance liquid chromatographic separation and direct spectrophotometric detection at 119 nm. Extraction can be checked by the use of an internal standard. The reproducibility, recovery and separation of fractions were satisfactory.
Hôpital Saint-Joseph, Laboratoire de Biochimie, Paris, France.
D
D
Labbé
M F
MF
Gerhardt
A
A
Myara
C
C
Vercambre
F
F
Trivin
D001647:Bile Acids and Salts / Q000097:blood*; D001651:Cholestasis, Extrahepatic / Q000097:blood / Q000188:drug therapy; D002851:Chromatography, High Pressure Liquid; D006001:Glycoconjugates / Q000097:blood*; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000188:drug therapy; D014580:Ursodeoxycholic Acid / Q000627:therapeutic use
D001647:Bile Acids and Salts; D006001:Glycoconjugates; D014580:Ursodeoxycholic Acid