In vitro sensitivity of immature human mast cells to chemotherapeutic agents.
Cells from the human immature mast cell line, HMC-1, were tested for their sensitivity to 11 chemotherapeutic agents by changes in viability and incorporation of tritiated thymidine ([3H]-thymidine) after 72 h of in vitro drug exposure. Doxorubicin hydrochloride and cytosine arabinoside were the most active agents against HMC-1 cells at the concentrations tested. Doxorubicin hydrochloride inhibited the incorporation of [3H]-thymidine and decreased the viability of HMC-1 cells by greater than 90% at a concentration of 0.06 microgram/ml. Cytosine arabinoside inhibited the incorporation of [3H]-thymidine and decreased the viability by greater than 95% at a concentration of 0.1 microgram/ml. A clone of HMC-1 cells, A4, with an enhanced percentage (70-80%) of metachromatically staining cells was equally sensitive to these two agents; however, A4 cells were more sensitive to vinblastine sulfate and less sensitive to methylprednisolone than was the parent cell line. Both HMC-1 cells and A4 cells showed approximately equal sensitivity to etoposide and to mitomycin. These results show that human mast cells are susceptible in vitro to a number of commonly used chemotherapeutic drugs.
Division of Allergic Diseases and Internal Medicine, Mayo Clinic, Rochester, Minn.
J H
JH
Butterfield
D A
DA
Weiler
D000970:Antineoplastic Agents / Q000633:toxicity*; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects*
D000970:Antineoplastic Agents