Use of colloidal gold in diagnostic surgical pathology.
Colloidal gold immuno-electron microscopy is a powerful tool for defining antigenicity at the subcellular level. Such studies permit correlation with cell fractionation studies. They also allow one to assess the specificity of a particular antibody. The most useful reagent for immuno-electron microscopy is colloidal gold stabilized by a binding protein, either staphylococcal protein A or immunoglobulin. This method permits highly discrete labeling, and the system is useful for most antibodies used in diagnostic pathology.
Department of Pathology, Pennsylvania Hospital, Philadelphia 19107.
M J
MJ
Warhol
D000941:Antigens / Q000032:analysis; D004848:Epithelium / Q000276:immunology; D006046:Gold; D006801:Humans; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000276:immunology / Q000648:ultrastructure; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D010340:Pathology, Surgical / Q000379:methods*; D011467:Prostate / Q000276:immunology / Q000378:metabolism
D000941:Antigens; D006046:Gold