Cloning and expression of Staphylococcus saprophyticus urease gene sequences in Staphylococcus carnosus and contribution of the enzyme to virulence.
The urease gene of Staphylococcus saprophyticus CCM883 was cloned and expressed in Staphylococcus carnosus TM300. In vitro translation of the cloned DNA sequences revealed six polypeptides (of 70, 47, 29, 27, 20, and 17 kilodaltons) that were associated with enzyme activity. Introduction of the cloned genes into a urease-negative mutant of S. saprophyticus restored the virulence of this strain, confirming our previous suggestion (S. Gatermann, J. John, and R. Marre, Infect. Immun. 57:110-116, 1989) that this enzyme is a major virulence factor of the organism and contributes mainly to cystopathogenicity.
Gatermann
S
S
Institut für Medizinische Mikrobiologie, Medizinische Universität, Lübeck, Federal Republic of Germany.
Marre
R
R
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003556:Cystitis / Q000201:enzymology / Q000382:microbiology; D005798:Genes, Bacterial; D010957:Plasmids; D014176:Protein Biosynthesis; D051381:Rats; D015183:Restriction Mapping; D013203:Staphylococcal Infections / Q000201:enzymology / Q000382:microbiology; D013210:Staphylococcus / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D014510:Urease / Q000235:genetics* / Q000502:physiology; D001743:Urinary Bladder / Q000382:microbiology; D014774:Virulence
D001426:Bacterial Proteins; D014510:Urease