Continuous-wave ultrasound and neoplastic transformation in vitro.
C3H/10T1/2 cells in suspension were assayed using an initiation-promotion protocol for neoplastic transformation induced by continuous-wave ultrasound. Cells were insonated at 1.765 MHz for 40 min. Two ultrasonic intensities were used: 1.3 and 2.6 W/cm2 spatial average. The first intensity was found to be noncytotoxic; the second resulted in immediate lysis of 20% of the cells, followed by the clonogenic survival of 64% of the remaining cells. Ultrasound was delivered alone or in combination with X-rays (2 Gy, 240 kVp given before ultrasound), and/or 12-O-tetradecanoyl-phorbol-13-acetate (TPA, 0.1 microgram/ml post-irradiation). Under all treatment conditions, there was no effect of ultrasound on transformation at the 95% confidence level.
Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD 21201.
G H
GH
Harrison
E K
EK
Balcer-Kubiczek
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000209:etiology*; D002478:Cells, Cultured; D003043:Cocarcinogenesis; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D051379:Mice; D008809:Mice, Inbred C3H; D009381:Neoplasms, Radiation-Induced; D013755:Tetradecanoylphorbol Acetate; D014465:Ultrasonics / Q000009:adverse effects*
D013755:Tetradecanoylphorbol Acetate