In vitro sulfation of sublingual salivary gland mucin: structures of 35S-labeled oligosaccharides.
The enzyme which catalyzes the transfer of sulfate ester group from 3'-phosphoadenosine-5'-phosphosulfate to salivary mucus glycoprotein was located in the detergent extract of the Golgi-rich membrane fraction of rat sublingual salivary glands. Alkaline borohydride reductive cleavage of the synthesized 35S-labeled glycoprotein led to the liberation of the label into the reduced acidic oligosaccharide fraction. A 90.3% of the total label was found incorporated in two oligosaccharides. These were identified in order of abundance as sulfated penta- and heptasaccharides. The pentasaccharide was characterized as SO3H,6G1cNAc beta 1,3Ga1 beta 1, 4G1cNAc beta 1,3(NeuAc alpha 2,6)Ga1NAc-01, and the heptasaccharide as SO3H,6G1cNAc beta 1,3Ga1 beta 1,4G1cNAc beta 1,3Ga1 beta 1,4 G1cNAc beta 1,3(NeuAc alpha 2,6)Ga1NAc-01.
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2425.
A
A
Slomiany
K
K
Okazaki
J
J
Piotrowski
B L
BL
Slomiany
D000818:Animals; D002236:Carbohydrate Conformation; D002855:Chromatography, Thin Layer; D006023:Glycoproteins / Q000378:metabolism; D006868:Hydrolysis; D009077:Mucins / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis*; D051381:Rats; D012469:Salivary Glands / Q000201:enzymology / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D015238:Sulfotransferases / Q000378:metabolism*
D006023:Glycoproteins; D009077:Mucins; D009844:Oligosaccharides; D015238:Sulfotransferases; C042860:glycoprotein sulfotransferase