Expression of VH11-gene family in hybridoma collections from peritoneum and spleen: differential correlation with BrMRBC reactivity.
Hybridoma collections from spleen or peritoneal cells of newborn or adult individuals were screened by RNA hybridization for expression of the VH11-gene family using a V-region probe VCP12, which encodes anti-BrMRBC antibodies. No VH11 expression was observed in hybridomas derived from newborn spleen cells in either BALB/c, NZB or (CBA/N x BALB/c) F1 mice (0/93). Adult NZB and BALB/c spleen cell collections contained only one hybridoma expressing VH11 (1/242). Interestingly, however, the VH11-positive hybridoma showed no anti-BrMRBC reactivity, while one anti-BrMRBC clone in the same collection expressed a Q52 VH gene. In contrast, hybridomas derived from peritoneal cells showed an absolute correlation between expression of VH11 genes and anti-BrMRBC reactivity (15/32). The high expression in the peritoneal cavity of such cells is likely the result of local positive selection.
Département d'Immunologie, Institut Pasteur, Paris.
P
P
Poncet
L
L
Reininger
A
A
Freitas
D
D
Holmberg
G
G
Dighiero
A
A
Coutinho
D000818:Animals; D000831:Animals, Newborn; D015342:DNA Probes; D004912:Erythrocytes / Q000276:immunology*; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D005810:Multigene Family; D010529:Peritoneal Cavity / Q000276:immunology; D013154:Spleen / Q000276:immunology
D015342:DNA Probes