Co-localization of RNAs coding for phenylethanolamine N-methyltransferase and proenkephalin A in bovine and ovine adrenals.
A 29-mer oligodeoxyribonucleotide probe, complementary to the coding region of bovine phenylethanolamine N-methyltransferase (PNMT) mRNA was synthesized. Characterization of this probe by Northern blot hybridization showed that it hybridized to a single band in RNA extracted from bovine and ovine adrenal medullae. The molecular size of this hybridized band was approximately 1.0-1.2 kb which is consistent with recently reported data on the molecular weight of bovine PNMT mRNA. In situ hybridization histochemistry was carried out with this probe on bovine and ovine adrenal sections and results compared on adjacent sections with a probe against proenkephalin A (ProEnk A) mRNA synthesized previously. Both showed a similar localization to the outer margin of cells in the adrenal medulla. The results of this study provide strong evidence at the level of mRNA expression that ProEnk A mRNA is expressed preferentially in the adrenaline synthesizing cells within the adrenal medulla. Further, it demonstrates the usefulness of a synthetic oligodeoxyribonucleotide probe for the study of PNMT gene expression.
Wan
D C
DC
Department of Biochemistry University of Melbourne, Parkville, Vic. (Australia).
Scanlon
D
D
Choi
C L
CL
Bunn
S J
SJ
Howe
P R
PR
Livett
B G
BG
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004745:Enkephalins / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012756:Sheep
D004745:Enkephalins; D009841:Oligonucleotides; D011498:Protein Precursors; D012333:RNA, Messenger; C029992:proenkephalin; D010625:Phenylethanolamine N-Methyltransferase