Plasma thromboxane B2 levels and thromboxane B2 production by platelets are increased in patients during spinal and epidural anesthesia.
Concentrations of thromboxane (Tx) B2 in plasma and its production by platelets were measured in 20 spinal and 10 epidural anesthesia patients scheduled for small operations in the lower extremities. The main metabolite of prostacyclin, 6-keto-PGF1 alpha and prostaglandin (PG) E2 in plasma were also determined. Plasma TxB2 and TxB2 production by platelets increased during both spinal and epidural anesthesia. Plasma TxB2 levels also remained elevated 1 h after anesthesia. The plasma concentrations of 6-keto-PGF1 alpha and PGE2 did not change during spinal or epidural anesthesia. In in vitro studies, only low concentrations of lidocaine (0.5-1.0 micrograms/ml) and bupivacaine (0.5-3.0 micrograms/ml) increased platelet TxB2 production. In platelet rich plasma, neither lidocaine nor bupivacaine in concentrations of 0.5-3.0 micrograms/ml caused constant changes in ADP-induced platelet aggregation, but they inhibited it in toxic concentrations (12 micrograms/ml). The results suggest that the increased TxB2 plasma levels and platelet TxB2 production during regional anesthesia are not caused by local anesthetics itself but by other factors, e.g. tissue trauma. In clinically found concentrations, local anesthetics do not cause any constant changes in platelet aggregation.
Department of Anesthesia, Tampere University Central Hospital, Finland.
S
S
Kaukinen
R
R
Ojanen
L
L
Kaukinen
E
E
Seppälä
H
H
Vapaatalo
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000328:Adult; D000767:Anesthesia, Epidural; D000775:Anesthesia, Spinal; D001792:Blood Platelets / Q000378:metabolism*; D002045:Bupivacaine / Q000494:pharmacology; D015232:Dinoprostone / Q000097:blood; D005260:Female; D006801:Humans; D008012:Lidocaine / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D013929:Thromboxane B2 / Q000096:biosynthesis / Q000097:blood*; D013997:Time Factors
D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D008012:Lidocaine; D015232:Dinoprostone; D002045:Bupivacaine