Binding of heat shock proteins to the avian progesterone receptor.
The protein composition of the avian progesterone receptor was analyzed by immune isolation of receptor complexes and gel electrophoresis of the isolated proteins. Nonactivated cytosol receptor was isolated in association with the 90-kilodalton (kDa) heat shock protein, hsp90, as has been described previously. A 70-kDa protein was also observed and was shown by Western immunoblotting to react with an antibody specific to the 70-kDa heat shock protein. Thus, two progesterone receptor-associated proteins are identical, or closely related, to heat shock proteins. When the two progesterone receptor species, A and B, were isolated separately in the absence of hormone, both were obtained in association with hsp90 and the 70-kDa protein. However, activated receptor isolated from oviduct nuclear extracts was associated with the 70-kDa protein, but not with hsp90. A hormone-dependent dissociation of hsp90 from the cytosolic form of the receptor complex was observed within the first hour of in vivo progesterone treatment, which could explain the lack of hsp90 in nuclear receptor complexes. In a cell-free system, hsp90 binding to receptor was stabilized by molybdate but disrupted by high salt. These treatments, however, did not alter the binding of the 70-kDa protein to receptor. Association of the 70-kDa protein with the receptor could be disrupted by the addition of ATP at elevated temperatures (23 degrees C). The receptor-associated 70-kDa protein is an ATP-binding protein, as demonstrated by its affinity labeling with azido[32P]ATP. These results indicate that the two receptor-associated proteins interact with the progesterone receptor by different mechanisms and that they are likely to affect the structure or function of the receptor in different ways.
Department of Biochemistry and Molecular Biology, Mayo Medical School, Rochester, Minnesota 55905.
S L
SL
Kost
D F
DF
Smith
W P
WP
Sullivan
W J
WJ
Welch
D O
DO
Toft
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002645:Chickens; D003600:Cytosol / Q000378:metabolism; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism*; D007120:Immunochemistry; D066298:In Vitro Techniques; D007700:Kinetics; D008970:Molecular Weight; D010057:Oviducts / Q000378:metabolism; D011376:Progesterone-Binding Globulin / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism*
D006360:Heat-Shock Proteins; D011376:Progesterone-Binding Globulin; D011980:Receptors, Progesterone; D000255:Adenosine Triphosphate