A sensitive analytical procedure for the detection of N-nitrosamides via their alkylating activity.
Based on a method for trapping direct-alkylating intermediates with scavenger reagents possessing a nucleophilic side-chain and an N-nitroso group as a marker, a sensitive test system for the detection of N-nitrosamides and related compounds has been developed. N-Nitroso-N-tert-butylglycine is an effective scavenger reagent for this purpose. It reacts readily with diazoalkanes released from N-nitrosamides on treatment with alkali to form the corresponding esters which are analysed by gas-liquid chromatography using a thermal energy analyser. The method is easy to perform and specific for N-nitroso compounds that decompose to non-polar diazoalkanes on treatment with alkali. A determination limit of 2 ng solids/sample was established for methylated NTBG formed from N-methyl-N-nitro-sourea following treatment with alkali.
German Cancer Research Center, Institute for Toxicology and Chemotherapy, Heidelberg.
P
P
Mende
B
B
Spiegelhalder
R
R
Preussmann
D000478:Alkylation; D002273:Carcinogens / Q000032:analysis*; D002849:Chromatography, Gas; D007202:Indicators and Reagents; D008745:Methylation; D009603:Nitroso Compounds / Q000032:analysis*
D002273:Carcinogens; D007202:Indicators and Reagents; D009603:Nitroso Compounds; C013621:nitrosamides