The effects of lithogenic bile on gallbladder epithelium.
Prairie dogs were fed a 1.2% cholesterol diet for up to 24 weeks to evaluate the effects of lithogenic bile on the mucosa of the gallbladder. There was a progressive increase in the lithogenic index of the gallbladder bile (1.44 +/- 0.15 at 4 weeks, p less than 0.05). Fifty-five of 70 animals developed gallstones between the second and fourth week. Increasing stone burden was associated with a 27% (p less than 0.05) decrease in the electrical resistance of the epithelium and a 60% (p less than 0.05) decrease in net sodium transport when measured isotopically in an Ussing chamber (3 weeks). After 4 months, seven of ten animals developed inflammatory mucosal polyps characterized by a heavy infiltration of plasma cells into an expanded matrix. Cellular infiltration began as early as 2 weeks. These changes occurred without alterations in the ultrastructural appearance of the epithelium.
Moody
F G
FG
Department of Surgery, University of Texas Health Science Center, Houston.
Haley-Russell
D
D
Li
Y F
YF
Husband
K J
KJ
Weisbrodt
N W
NW
Dewey
R B
RB
D000042:Absorption; D000818:Animals; D001646:Bile / Q000032:analysis / Q000502:physiology*; D002769:Cholelithiasis / Q000209:etiology*; D002791:Cholesterol, Dietary / Q000494:pharmacology; D004594:Electrophysiology; D005704:Gallbladder / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D009092:Mucous Membrane / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D012589:Sciuridae; D012964:Sodium / Q000378:metabolism; D014867:Water / Q000378:metabolism
D002791:Cholesterol, Dietary; D014867:Water; D012964:Sodium