Electron microscopic evidence for endocytosis of superoxide dismutase by hepatocytes using protein-gold adducts.
Cu, Zn superoxide dismutase, an intracellular, carbohydrate-free protein, was found to bind to hepatocytes and endothelial cells by electron microscopy of liver treated with enzyme-gold conjugates. In cultured hepatocytes, the free enzyme, but not bovine serum albumin, competed for the binding, and at 37 degrees C, the gold-protein complexes were internalized in a typical process of receptor-mediated endocytosis.
Dini
L
L
Department of Biology, University of Rome Tor Vergata, Italy.
Rotilio
G
G
D000818:Animals; D002417:Cattle; D002451:Cell Compartmentation; D004705:Endocytosis; D004727:Endothelium / Q000378:metabolism; D006046:Gold; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism*
D006046:Gold; D013482:Superoxide Dismutase