Ontogeny and implication of pregnancy-associated agglutinin of the rat uterus.
The mannose/fructose-binding agglutinin from Day 1-7 post coitum (p.c.) rat uteri was purified on Concanavalin A. The specific haemagglutination activity peaked on Days 4 and 5 p.c. and a 1.4-fold increase in the yield was accompanied by a 10-12-fold increase in specific agglutination titre. The mannose-binding affinity of the protein also increased, but the highest fructose-binding affinity was found on Day 1 p.c., which may indicate a role of the protein in fructose concentration for utilization by the spermatozoa. Rats that were pseudopregnant, superovulated and pseudopregnant, and had one uterine horn ligated showed that, although a basal level of the protein was induced by the hormonal milieu, actual stimulation of the protein synthesis occurred in the presence of the fertilized ova.
Indian Institute of Chemical Biology, Calcutta.
P K
PK
Mukhopadhyay
M
M
Chowdhury
D000818:Animals; D002846:Chromatography, Affinity; D010064:Embryo Implantation; D005260:Female; D005632:Fructose / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*; D011270:Pregnancy, Animal / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000032:analysis* / Q000378:metabolism; D015053:Zygote / Q000502:physiology
D011257:Pregnancy Proteins; D005632:Fructose; D008358:Mannose