Identification of two transactivation domains in the mouse oestrogen receptor.
We have identified two discrete transactivation domains within the mouse oestrogen receptor whose relative activities vary according to the target promoter. One domain lies within the N-terminal region and is active in the absence of oestradiol. The second domain is contained within the C-terminal portion of the protein and depends upon oestrogen binding for its activity. The location and oestrogen dependence of this domain has been confirmed using chimaeric receptors containing the Lex A DNA binding domain. Although transactivation by the C-terminal domain is dependent upon ligand binding the analysis of receptor deletion mutants has demonstrated that these two functions are not entirely coincident.
Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, London, UK.
J A
JA
Lees
S E
SE
Fawell
M G
MG
Parker
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002872:Chromosome Deletion; D004958:Estradiol / Q000494:pharmacology*; D005786:Gene Expression Regulation; D005796:Genes / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011960:Receptors, Estrogen / Q000235:genetics*; D013629:Tamoxifen / Q000494:pharmacology; D014162:Transfection
D015345:Oligonucleotide Probes; D011960:Receptors, Estrogen; D013629:Tamoxifen; D004958:Estradiol