Method for reducing the rate of light emission from chemiluminescent and bioluminescent reactions using frozen reagents.
Frozen assay reagents have been used to reduce the rate of light emission from the rapid chemiluminescent acridinium ester and the bioluminescent firefly luciferase reactions. Melting of the assay reagent delays the initiation of the light emission, thus eliminating the need to initiate these rapid reactions by injection of the assay reagents in front of the photodetector.
Kricka
L J
LJ
Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104.
Ogden
S
S
Williams
T M
TM
Kant
J
J
D000166:Acridines; D005615:Freezing; D007202:Indicators and Reagents; D007700:Kinetics; D008156:Luciferases; D008163:Luminescent Measurements; D010781:Photography
D000166:Acridines; D007202:Indicators and Reagents; D008156:Luciferases