Decline in sialic acid composition of cellular membranes isolated from ovine corpora lutea during prostaglandin-induced luteolysis: apparent independence of autoimmune recognition.
Sialic acid was quantified in plasma membranes of corpora lutea isolated during prostaglandin (PG) F2 alpha-induced luteolysis in sheep. Concentrations of sialic acid within membranes decreased after injection of PGF2 alpha, and before signs of luteal regression (i.e., a decline in tissue concentrations of progesterone) were manifested. Removal of residues of sialic acid from luteal membranes was not associated with cellular binding of gamma globulin, as monitored by indirect immunofluorescence microscopy. We suggest that desialylation of luteal membranes could be an important aspect of the mechanism of luteolysis. Such a process does not appear to involve participation of autoantibody.
Department of Animal Science, University of Wyoming, Laramie 82071.
L E
LE
Steadman
G A
GA
Johnson
E L
EL
Belden
W J
WJ
Murdoch
D000818:Animals; D001323:Autoantibodies / Q000502:physiology*; D002462:Cell Membrane / Q000032:analysis; D003338:Corpus Luteum / Q000032:analysis* / Q000187:drug effects; D015237:Dinoprost / Q000494:pharmacology*; D005260:Female; D019158:N-Acetylneuraminic Acid; D012756:Sheep; D012794:Sialic Acids / Q000032:analysis*
D001323:Autoantibodies; D012794:Sialic Acids; D015237:Dinoprost; D019158:N-Acetylneuraminic Acid