Presence of 1-O-alk-1'-enyl-2-O-acetyl glycerophosphocholine (vinyl form of PAF) in perfused rat and guinea pig hearts.
1-O-Alk-1'-enyl-2-O-acetyl-glycerophosphocholine (vinyl form of PAF) was found with PAF in perfused rat and guinea pig hearts. The main molecular species of the vinyl form of PAF, after separation by reverse phase HPLC, were identified as 1-O-hexadec-, -octadec-, and -octadecen-1'-enyl-2-O-acetyl-GPCs (16:0, 18:0, and 18:1 vinyl forms of PAF) by mass spectrometry. The amounts of the predominant 16:0 species in rat and guinea pig hearts, respectively, were 46.4 and 22.5 ng per mg lipid-phosphorus of the original heart phospholipids.
Department of Medical Chemistry, Kansai Medical School, Osaka.
R
R
Nakayama
K
K
Saito
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008055:Lipids / Q000097:blood; D008297:Male; D009206:Myocardium / Q000032:analysis*; D010477:Perfusion; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D008055:Lipids; D010972:Platelet Activating Factor; C055475:1-O-hexadec-1'-enyl-2-acetyl-sn-glycero-3-phosphocholine