[Effects of salivary ultrafiltrates on the production of phenol from tyrosine by oral microorganisms].
The metabolism of oral microorganisms is known to correlate with oral diseases. Protein degradation by oral microorganisms is usually followed by the production of phenol from tyrosine. The purpose of this study is to clarify the effect of salivary ultrafiltrates [MW: less than 10,000, 1,000-10,000 (intermediate fraction), less than 1,000, less than 500] on the production or the decomposition of phenol by oral microorganisms. Salivary sediment and salivary ultrafiltrate, the molecular weight of which is less than 10,000 unless otherwise stated, were incubated at 37 degrees C for 24 hours with tyrosine or phenol at a final concentration of 2.5 mg/100 ml. After incubation, the phenol produced (or remained) was extracted with ether and determined spectrophotometrically. Phenol production by oral microorganisms was not seen in the non-dialyzable fraction of saliva even when tyrosine was added. It was confirmed that the presence of the salivary ultrafiltrates was essential for the production of phenol by oral microorganisms. Phenol production in the salivary ultrafiltrate was further increased with the addition of tyrosine or non-dialyzable fraction of saliva. Phenol disappeared within 48 or 72 hours after the salivary sediment was incubated with phenol. The decomposition of phenol was inhibited with the salivary ultrafiltrate. Phenol production was enhanced with the addition of Good's buffer. Optimum pH of phenol production by oral microorganisms in the ultrafiltrate of saliva was within the range from 6.5 to 7.0 in the presence of Good's buffer (MOPS). Phenol production decreased with the reduction of the molecular weight of the salivary ultrafiltrates. The phenol production was increased when the intermediate fraction was added to the ultrafiltrate (MW: less than 1,000). The increase of the phenol production was considered to be due to the peptides in the intermediate fraction.
Nagayama
M
M
Inoue
M
M
Mishiro
Y
Y
D001419:Bacteria / Q000378:metabolism*; D009055:Mouth / Q000382:microbiology; D019800:Phenol; D010636:Phenols / Q000096:biosynthesis*; D012463:Saliva / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism*; D014462:Ultrafiltration
D010636:Phenols; D019800:Phenol; D014443:Tyrosine