Two better cell lines for making hybridomas expressing specific T cell receptors.
Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be studied at a clonal level, without interference of the BW5147-derived receptor chains. In this study one of the variants has been useful in identifying the reactivity to allogeneic MHC Ag of BW5147 itself.
Howard Hughes Medical Institute, Department of Pediatrics, Denver, CO.
J
J
White
M
M
Blackman
J
J
Bill
J
J
Kappler
P
P
Marrack
D P
DP
Gold
W
W
Born
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002459:Cell Fusion / Q000379:methods; D002460:Cell Line; D002872:Chromosome Deletion; D006825:Hybridomas / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009154:Mutation; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D013945:Thymoma / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D011948:Receptors, Antigen, T-Cell