Direct evidence that the kinase activity of protein kinase C is involved in transcriptional activation through a TPA-responsive element.
In order to examine the involvement of protein kinase C (PKC) in the transcriptional activation of genes by TPA (12-0-tetradecanoyl phorbol 13-acetate) we have constructed a series of PKC expression plasmids. Transient expression of an active fragment of PKC in rat fibroblasts resulted in the transcriptional activation of a TRE (TPA-responsive element)-CAT chimeric gene which contains various repetitions of collagenase TREs. These provide the first direct evidence that kinase activity of PKC is involved in TPA-induced transcriptional activation through TRE.
Department of Molecular Biology, Tokyo Metropolitan Institute of Medicl Science, Japan.
A
A
Hata
Y
Y
Akita
Y
Y
Konno
K
K
Suzuki
S
S
Ohno
D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004789:Enzyme Activation; D005347:Fibroblasts; D005786:Gene Expression Regulation; D010957:Plasmids; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic; D014162:Transfection
D015500:Chloramphenicol O-Acetyltransferase; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate