Decreased DNA repair in familial Alzheimer's disease.
Alterations in the capacity of a cell to repair DNA lesions play an important role in a number of human diseases. We and others have demonstrated defective DNA repair of alkylation damage in cells from patients with Alzheimer's disease. It has been hypothesized that this defect is related to the cause of Alzheimer's disease and results in the accumulation of lesions in the central nervous system neurons. One prediction of this hypothesis is that in dominantly inherited Alzheimer's disease, the repair defect will be present in half of the offspring of affected patients long before they develop symptoms of the disease. In order to test the hypothesis that decreased DNA repair is responsible for familial Alzheimer's disease and their at-risk offspring we have studied DNA repair in these individuals after exposure of lymphoblasts to alkylating agents. Our results indicate that cell lines from affected patients repair significantly less damage in 3 h than cell lines from healthy controls. A small number of at-risk individuals were also studied and some of these had lower levels of repair, although more cell lines from individuals in this group must be studied. These findings provide further support for defective DNA repair playing a role in the pathogenesis of Alzheimer's disease.
Jones
S K
SK
Neurology Department, University of Vermont College of Medicine, Burlington 05401.
Nee
L E
LE
Sweet
L
L
Polinsky
R J
RJ
Bartlett
J D
JD
Bradley
W G
WG
Robison
S H
SH
D000328:Adult; D000368:Aged; D000544:Alzheimer Disease / Q000235:genetics*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004247:DNA / Q000187:drug effects / Q000302:isolation & purification; D004260:DNA Repair; D005260:Female; D006801:Humans; D008297:Male; D008741:Methyl Methanesulfonate; D008769:Methylnitronitrosoguanidine; D008875:Middle Aged
D008769:Methylnitronitrosoguanidine; D004247:DNA; D008741:Methyl Methanesulfonate