Isolation and sequencing of a cDNA clone encoding acid phosphatase in rat liver lysosomes.
A full length cDNA for acid phosphatase in rat liver lysosomes was isolated and sequenced. The predicted amino acid sequence comprises 423 residues (48,332 Da). A putative signal peptide of 30 residues is followed by the NH2-terminal sequence of lysosomal acid phosphatase (45,096 Da). The deduced NH2-terminal 18-residue sequence is identical with that determined directly for acid phosphatases purified from the rat liver lysosomal membranes. The primary structure deduced for acid phosphatase contains 9 potential N-glycosylation sites and a hydrophobic region which could function as a transmembrane domain. It exhibits 89% and 67% sequence similarities in amino acids and nucleic acids, respectively, to human lysosomal acid phosphatase. The amino acid sequence of the putative transmembrane segment shows a complete similarity to that of the human enzyme. Northern blot hybridization analysis identified a single species of acid phosphatase mRNA (2.2 kbp in length) in rat liver.
Himeno
M
M
Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Fujita
H
H
Noguchi
Y
Y
Kono
A
A
Kato
K
K
D000135:Acid Phosphatase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D008565:Membrane Proteins / Q000235:genetics / Q000648:ultrastructure; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping
D008565:Membrane Proteins; D004247:DNA; D000135:Acid Phosphatase