Simple high-performance liquid chromatographic method for the measurement of toloxatone in rabbit cerebrospinal fluid and plasma.
A simplified, rapid high-performance liquid chromatographic procedure has been developed for the measurement of toloxatone in rabbit plasma and cerebrospinal fluid. The method involves a single-step extraction of the alkalinized sample with diethyl ether and analysis of the evaporated extract on a C8 column. Detection was performed by ultraviolet absorbance monitored at 240 nm. The overall run-time of the assay was 8 min at a flow-rate of 1 ml min-1. The sensitivity limit of toloxatone was 70 ng ml-1 at a signal-to-noise ratio of 3:1 in rabbit cerebrospinal fluid and plasma. The assay has been used to define plasma toloxatone concentration-time profiles and to quantitate cerebrospinal fluid toloxatone levels after a single intravenous injection in rabbits.
Laboratoire de Pharmacologie, U.F.R. de Pharmacie, Reims, France.
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Vistelle
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Lamiable
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Zinzou
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008996:Monoamine Oxidase Inhibitors; D010080:Oxazoles / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics*; D023303:Oxazolidinones; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
D008996:Monoamine Oxidase Inhibitors; D010080:Oxazoles; D023303:Oxazolidinones; C010798:toloxatone