Differential oligoclonal band patterns on polyvinyldifluoride membranes.
We report a novel observation which has been reproducibly noted whilst studying immunoglobulin G binding to antigen immobilized on polyvinyldifluoride membranes. In some samples we have observed a difference between the pattern of oligoclonal bands on the front surface when compared to the reverse side of the membrane. We postulate that this observation results from differing affinities of the specific antibody binding to the antigen immobilised on the membrane. High affinity antibody will bind to antigen on the surface of the membrane next to the gel, while lower affinity antibody appears to diffuse through to the reverse side of the membrane.
Special Chemical Pathology, Institute of Neurology, London, U.K.
R W
RW
Luxton
E J
EJ
Thompson
D000915:Antibody Affinity; D003101:Collodion; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D011145:Polyvinyls; D005465:Fluorocarbon Polymers
D003101:Collodion; D007074:Immunoglobulin G; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers