An enzyme-linked immunosorbent assay (ELISA) for urinary growth hormone suitable for use in the routine laboratory. The Wessex Growth Study.
An avidin-biotin enzyme-linked immunoassay was developed to measure human growth hormone (hGH) in urine. The assay was validated in terms of sensitivity, specificity and reproducibility. Parallelism was demonstrated between the standard curve (4-500 pg/ml) and serially concentrated urine. There was no interference from 125 ng/ml of adrenocorticotrophin, follicle stimulating hormone, luteinising hormone, prolactin or thyroid stimulating hormone. Recovery of exogenous human growth hormone in urine ranged from 91 to 103%. Intra- and interassay variations were less than 10% and sensitivity was 1.4 pg/ml. Application to timed overnight urine samples from both normal and short children or from acromegalic patients suggests the assay may be useful for simple, non-invasive assessment of growth hormone secretion. Moreover, use of standard laboratory equipment and commercially available components, together with the avoidance of radioisotopes, facilitate its use as a routine screening assay.
Department of Medicine II, Southampton General Hospital, U.K.
H
H
Bullen
T J
TJ
Wilkin
D001360:Avidin; D001710:Biotin; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D013006:Growth Hormone / Q000652:urine*; D006801:Humans
D001360:Avidin; D001710:Biotin; D013006:Growth Hormone