Biocompatibility testing of a posterior composite and dental cements using a new organ culture model.
Mandibular first molars from day 14 and 17 mouse embryos were cultured in vitro for 7 days in chemically defined Pratt's medium in a submerged culture system. Ameloblast and odontoblast polarization and morphogenesis occurred in the control tooth germs. Discs of Occlusin, Silicate, ChemFil, IRM and Dycal were exposed to the culture system as either fresh material, leached discs (by pre-incubation in media) or the leachate from the incubated discs. Their effects on dental differentiation were assessed histologically. Day 17 tooth germs were slightly more sensitive to the effects of the exogenous agents than day 14 tooth germs. In general, silicate and ChemFil were toxic, IRM was slightly less toxic and the major effect was from the leachate. Dycal was toxic but most of this effect resulted from pH changes in the leachate. Occlusin was the most biocompatible material tested. Only a very mild adverse effect was detected, and this appeared to be caused by an agent (not a pH change) released into the leachate.
Hetem
S
S
Jowett
A K
AK
Ferguson
M W
MW
D000538:Aluminum Silicates / Q000633:toxicity; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D002126:Calcium Hydroxide / Q000633:toxicity; D003188:Composite Resins / Q000633:toxicity; D003738:Dental Cements / Q000633:toxicity; D005899:Glass Ionomer Cements / Q000633:toxicity; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D008903:Minerals / Q000633:toxicity; D009924:Organ Culture Techniques; D012823:Silicate Cement / Q000633:toxicity; D014083:Tooth Germ / Q000187:drug effects / Q000473:pathology; D014520:Urethane / Q000633:toxicity
D000538:Aluminum Silicates; D001672:Biocompatible Materials; D003188:Composite Resins; D003738:Dental Cements; D005899:Glass Ionomer Cements; D008903:Minerals; C056413:Occlusin; D012823:Silicate Cement; D014520:Urethane; C025702:Dycal; C039733:Chemfil; D002126:Calcium Hydroxide