Senescence-induced alteration in cell surface carbohydrates correlated using proton NMR spectroscopy and a lectin-based affinity-binding assay.
Changes in the cell surface oligosaccharides in human fetal lung fibroblasts (IMR-90) are studied as the cells progress to senescence using nuclear magnetic resonance spectroscopy (NMR) and a biochemical assay. A lectin-based affinity-binding technique is used which measures the organization of carbohydrates on the cell surface. Proton NMR studies of the water in samples of frozen cell suspensions of young and old cells provide information on the local dynamics of the cell surface by monitoring the motion of bound water. Changes in the lectin binding density and affinity class distribution correlate with a decrease in the water proton linewidth in frozen cells. These observations reflect alterations in the conformation or structure of the cell surface oligosaccharides and local constituent water.
Center for Non-Invasive Diagnosis, University of New Mexico School of Medicine, Albuquerque 87131.
S C
SC
Busse
P L
PL
Mann
R H
RH
Griffey
D000440:Butanols / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure*; D002846:Chromatography, Affinity / Q000379:methods; D005615:Freezing; D006801:Humans; D007424:Intracellular Fluid / Q000032:analysis; D037102:Lectins; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009844:Oligosaccharides / Q000302:isolation & purification / Q000502:physiology*; D020002:tert-Butyl Alcohol
D000440:Butanols; D037102:Lectins; D009844:Oligosaccharides; D020002:tert-Butyl Alcohol