New galactosyllactose containing alpha-glycosidic linkage isolated from ovine (Booroola dorset) colostrum.
Three trisaccharides, tetra-, penta-, hexa- and certain higher oligosaccharides were obtained from ovine colostrum as free forms. The chemical structure of the three trisaccharides were determined by methylation and 13C-NMR analyses to be as follows: Gal alpha 1-3Gal beta 1-4Glc, Gal beta 1-3Gal beta 1-4Glc (3'-galactosyllactose) and Gal beta 1-6Gal beta 1-4Glc (6'-galactosyllactose). Gal alpha 1-3Gal beta 1-4Glc, which had been confirmed as the oligosaccharide portion of a glycolipid prepared biosynthetically from rat spleen or bone marrow, has been identified for the first time from natural sources as a free form. The trisaccharide containing alpha-galactosyl unit is a novel compound in mammalian milk.
Laboratory of Dairy Chemistry, Faculty of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Japan.
T
T
Urashima
T
T
Saito
J
J
Nishimura
H
H
Ariga
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002417:Cattle; D003126:Colostrum / Q000032:analysis*; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D011247:Pregnancy; D014312:Trisaccharides / Q000302:isolation & purification*
D002247:Carbon Isotopes; D014312:Trisaccharides; C061732:3'-galactosyllactose