Identification of Nsp100 as elongation factor 2 (EF-2).
The nerve growth factor-sensitive phosphoprotein from PC12 cells, previously designated Nsp100, has been shown to be elongation factor 2 (EF-2). The criteria used for this identification include: (i) similarity of N-terminal sequence; (ii) phosphorylation by the same kinase; (iii) ADP-ribosylation mediated by diphtheria toxin; (iv) comparable function in cell-free protein synthesis. According to these criteria, Nsp100 and EF-2 are identical and the kinase that phosphorylates Nsp100 in PC12 cells is calcium/calmodulin kinase III.
Koizumi
S
S
Section on Growth Factors, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Ryazanov
A
A
Hama
T
T
Chen
H C
HC
Guroff
G
G
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis*; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000032:analysis*; D010673:Pheochromocytoma; D010750:Phosphoproteins / Q000032:analysis; D010766:Phosphorylation; D051381:Rats; D014407:Tumor Cells, Cultured
D009419:Nerve Tissue Proteins; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D010750:Phosphoproteins; D000246:Adenosine Diphosphate Ribose