Production and characterization of four monoclonal antibodies specific for human interferon-alpha-1 and -alpha-2.
Four monoclonal antibodies against HuIFN-alpha-1 and -alpha-2 were produced by the lymphocyte hybridoma technique. The screening for monoclonal anti-IFN antibodies was performed by two different procedures permitting the detection of neutralizing and non-neutralizing antibodies. The first antibody screening in the supernatants of hybridoma clones was carried out by an immunodot ELISA using a novel chromogen, 2-bromo-1-naphthol, for the immunoperoxidase reaction. The second screening was performed with a solid-phase bioassay. The monoclonal antibodies IIG11 and IVH2 react specifically with HuIFN-alpha-1 independently of its origin, natural or recombinant. The mAb IIG11 recognizes also an epitope on a 27 kDa interferon, probably HuIFN-alpha-8. The mAb IIG7 binds to HuIFN-alpha-2 only, whereas the mAb IIB2 binds to HuIFN-alpha-2 and also to the 27 kDa interferon and to a 25 kDa interferon, not yet further characterized. These antibodies can be used successfully for immunopurification as well as for quantification of the corresponding interferons.
Noll
F
F
Central Institute of Molecular Biology, Academy of Sciences of GDR, Berlin-Buch.
Handschack
W
W
Eichmann
E
E
Gaestel
M
M
Schneider
F
F
Kuhl
R
R
Löster
K
K
D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000937:Antigen-Antibody Reactions; D001681:Biological Assay; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007370:Interferon Type I / Q000032:analysis* / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D008970:Molecular Weight; D009500:Neutralization Tests; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D011994:Recombinant Proteins