A new approach for measuring the erythrocyte life span with a nonradioisotope.
The possibility of substituting a nonradioactive isotope of chromium for 51Cr was studied. Blood, from five dogs with varying erythrocyte life spans, was labeled with 50Cr and 51Cr and transfused autologously. Erythrocyte-bound 50Cr was determined by inductively coupled argon plasma mass spectrometry and 51Cr by measuring radioactivity. Erythrocyte life span determination derived from the two isotopes did not differ significantly. Our results suggest that 50Cr can provide a nonradioactive alternative to 51Cr for labeling erythrocytes for life span studies.
Department of Pathology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
M
M
Dever
J E
JE
Smith
D W
DW
Hausler
D000818:Animals; D002857:Chromium / Q000032:analysis*; D002859:Chromium Isotopes; D002860:Chromium Radioisotopes; D004285:Dogs; D004905:Erythrocyte Aging; D006207:Half-Life; D007553:Isotope Labeling; D013058:Mass Spectrometry; D013048:Specimen Handling
D002859:Chromium Isotopes; D002860:Chromium Radioisotopes; D002857:Chromium