Fish viruses: isolation of an icosahedral cytoplasmic deoxyribovirus from sheatfish (Silurus glanis).
An icosahedral cytoplasmic deoxyvirus has been isolated from moribund sheatfish (Silurus glanis) fry of a commercial warm water recirculation aquaculture unit with cumulative mortalities of up to 100%. The agent replicated in BF-2 and in FHM cells at 20-30 degrees C producing cytoplasmatic inclusion bodies followed by lysis of the cells. The DNA containing virus proved to be labile to chloroform. Infected BF-2 cells revealed hexagonal particles in the cytoplasm measuring about 125-135 nm in diameter. The virus consisted of a central electron-dense core and a electron-translucent zone. The isolate shares characteristics with the Iridoviridae.
Ahne
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W
Schlotfeldt
H J
HJ
Thomsen
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I
D000818:Animals; D002397:Catfishes; D004267:DNA Viruses / Q000302:isolation & purification* / Q000648:ultrastructure; D005393:Fish Diseases / Q000382:microbiology*; D007497:Iridoviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D008854:Microscopy, Electron; D014777:Virus Diseases / Q000382:microbiology / Q000662:veterinary*