Effect of milk on fibronectin and collagen type I binding to Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis.
Tryptic soy broth (TSB)-grown cells of Staphylococcus aureus isolated from acute and chronic bovine mastitis bound mainly 125I-fibronectin (Fn) [corrected], whereas strains of nine species of coagulase-negative staphylococci showed a predominant interaction with 125I-collagen (Cn) [corrected] type I. A particle agglutination assay (PAA) was used to examine the interaction of coagulase-negative staphylococci with 125I-Fn and 125I-Cn immobilized on latex. All 368 coagulase-negative staphylococci demonstrated high 125I-Cn and moderate to low 125I-Fn interactions in the PAA. Cn-PAA reactivity was high among strains of Staphylococcus xylosus (84.2%), Staphylococcus simulans (77.8%), Staphylococcus epidermidis (76.7%), and Staphylococcus hyicus (74.3%), whereas all six Staphylococcus capitis strains clumped Cn-PAA reagent. Incubating TSB-grown cells in 10% skim milk for 1 h decreased the 125I-Fn- and 125I-Cn-binding affinity in most of the S. aureus and coagulase-negative staphylococci, while growth in 10% skim milk for 18 h resulted in more than 90% decrease or complete loss of interaction with these proteins. Decreased 125I-Fn binding in the presence of milk was correlated with protease production but not with 125I-Cn binding.
Department of Medical Microbiology, University of Lund, Sweden.
J
J
Miedzobrodzki
A S
AS
Naidu
J L
JL
Watts
P
P
Ciborowski
K
K
Palm
T
T
Wadström
D000208:Acute Disease; D000372:Agglutination Tests; D000818:Animals; D002417:Cattle; D002908:Chronic Disease; D003094:Collagen / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000378:metabolism*; D011485:Protein Binding; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013210:Staphylococcus / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism
D005353:Fibronectins; D003094:Collagen