Single-step purification and h.p.l.c. analysis of glutathione transferase 8-8 in rat tissues.
GSSG selectively elutes two GSH transferases from a mixture of rat GSH transferases bound to a GSH-agarose affinity matrix. One is a form of GSH transferase 1-1 and the other is shown to be GSH transferase 8-8. By using tissues that lack this form of GSH transferase 1-1 (e.g. lung), GSH transferase 8-8 may thus be purified from cytosol in a single step. Quantitative analysis of the tissue distribution of GSH transferase 8-8 was obtained by h.p.l.c.
Cancer Research Campaign Molecular Toxicology Group, Department of Biochemistry, University College and Middlesex School of Medicine, London, U.K.
D J
DJ
Meyer
E
E
Lalor
B
B
Coles
A
A
Kispert
P
P
Alin
B
B
Mannervik
B
B
Ketterer
D000818:Animals; D002847:Chromatography, Agarose; D002851:Chromatography, High Pressure Liquid; D005982:Glutathione Transferase / Q000032:analysis / Q000302:isolation & purification*; D007527:Isoenzymes / Q000032:analysis / Q000302:isolation & purification*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000201:enzymology
D007527:Isoenzymes; D005982:Glutathione Transferase