Amino-acid sequence of the short subfragment-2 in adult chicken skeletal muscle myosin.
The amino-acid sequence of a short subfragment-2 in the amino-terminal portion of subfragment-2 (S-2) derived from adult chicken skeletal muscle myosin was completely determined. Peptides cleaved by cyanogen bromide and by lysyl endopeptidase of S-carboxymethylated S-2, and hydrolytic peptides obtained with trypsin or dilute acetic acid of larger CNBr fragments were isolated and sequenced. This region was composed of 257 amino-acid residues, and hydrophobic and charged residue repeat units were found highly conserved and with a periodicity in 7 or 28 residues. This sequence of the short S-2 fragment of chicken skeletal muscle myosin was compared with the sequence of chicken and rat embryonic skeletal muscle myosins, rabbit skeletal and rabbit cardiac muscle myosin (alpha-myosin heavy chain), and 95.3%, 86.8%, 89.9% and 94.2% sequence identities were observed, respectively.
School of Allied Medical Sciences, Nagasaki University.
B
B
Watanabe
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens; D003488:Cyanogen Bromide; D008969:Molecular Sequence Data; D009132:Muscles; D015879:Myosin Subfragments; D009218:Myosins / Q000302:isolation & purification; D010446:Peptide Fragments / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases
D000596:Amino Acids; D015879:Myosin Subfragments; D010446:Peptide Fragments; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase; D009218:Myosins; D003488:Cyanogen Bromide