A method of human diaphorase locus 3 phenotyping in human semen by isoelectric focusing.
A rapid method for the separation of diaphorase (DIA 3) isozymes in the pH range 6-8 is presented. Isoelectric focusing (IEF) of DIA 3 on 0.2 mm thick polyacrylamide gels took 60 min at 5 degrees C. Separated bands are sharp and common phenotypes are readily distinguishable. The method combines high resolution, speed, reproducibility and reagent economy with simplicity.
Institute of Forensic Medicine, Medical Academy Gdańsk, Poland.
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Pawlowski
D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis / Q000235:genetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D008297:Male; D010641:Phenotype; D012661:Semen / Q000201:enzymology*
D007527:Isoenzymes; D008058:Dihydrolipoamide Dehydrogenase