Conductometric transducers for enzyme-based biosensors.
The use of alternating current conductometric transducers in biosensing devices has been investigated for urea and D-amino acid sensors using the enzyme systems urease and D-amino acid oxidase/catalase. Transducers with copper and platinum electrodes were constructed and characterized, and two enzyme immobilization methods were tested. Detection limits of 1 x 10(-6)M and linear ranges of 2 orders of magnitude were routinely achieved for these model sensors with enzymes covalently immobilized on collagen films.
S R
SR
Mikkelsen
G A
GA
Rechnitz
D000596:Amino Acids / Q000032:analysis; D015374:Biosensing Techniques; D004563:Electrochemistry; D004798:Enzymes; D006863:Hydrogen-Ion Concentration
D000596:Amino Acids; D004798:Enzymes