Determination of vitamin B6 derivatives in foods and biological materials by reversed-phase HPLC.
Through use of a simplified analyzing system, seven vitamin B6 derivatives were determined with a satisfactory sensitivity and precision. This system consisted of a single reversed-phase ODS column with a fluorescence detector employing an isocratic solvent system. Each vitamin B6 derivative in some foods and biological materials was determined, based on the measurement of the integrated peak area. The data obtained by this method were compared with those obtained from a bioassay by Saccharomyces uvarum ATCC 9080, after acid hydrolysis of these materials.
Institute for Food Science, Faculty of Agriculture, Gifu University, Japan.
T
T
Toukairin-Oda
E
E
Sakamoto
N
N
Hirose
M
M
Mori
T
T
Itoh
H
H
Tsuge
D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D005519:Food Preservation / Q000379:methods; D005615:Freezing; D011736:Pyridoxine / Q000031:analogs & derivatives* / Q000032:analysis*; D051381:Rats; D012440:Saccharomyces / Q000254:growth & development
D011736:Pyridoxine