WS-9659 A and B, novel testosterone 5 alpha-reductase inhibitors isolated from a Streptomyces. I. Taxonomy, fermentation, isolation, physico-chemical characteristics.
WS-9659 A and B, produced by Streptomyces sp. No. 9659, were extracted from cultured broth, purified by solvent extraction followed by chromatography on silica gel and then isolated as prisms (C22H24N2O, mp 161 approximately 162 degrees C, C22H23N2OCl, mp 152 approximately 153 degrees C). WS-9659 A and B have testosterone 5 alpha-reductase inhibitory activity. The IC50 values of WS-9659 A and B for partially purified rat prostate testosterone 5 alpha-reductase were 5.0 x 10(-7) M and 1.0 x 10(-5) M, respectively.
Exploratory Research Laboratory, Fujisawa Pharmaceutical Co., Ltd., Ibaraki, Japan.
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Nakayama
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Yagi
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M
Tanaka
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Kiyoto
M
M
Okuhara
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M
Kohsaka
D058891:5-alpha Reductase Inhibitors; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005285:Fermentation; D066298:In Vitro Techniques; D008297:Male; D010619:Phenazines / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D011467:Prostate / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*
D058891:5-alpha Reductase Inhibitors; D000900:Anti-Bacterial Agents; D010619:Phenazines; C060460:lavanducyanin; C060647:WS 9659 B