Identification of the cystic fibrosis gene: chromosome walking and jumping.
An understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA.
Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
J M
JM
Rommens
M C
MC
Iannuzzi
B
B
Kerem
M L
ML
Drumm
G
G
Melmer
M
M
Dean
R
R
Rozmahel
J L
JL
Cole
D
D
Kennedy
N
N
Hidaka
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular / Q000379:methods; D006224:Cricetinae; D003550:Cystic Fibrosis / Q000235:genetics*; D015342:DNA Probes; D005807:Genes, Overlapping; D005808:Genes, Recessive; D005819:Genetic Markers; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping / Q000379:methods
D015342:DNA Probes; D005819:Genetic Markers