Immunocytochemical demonstration of a novel system of neuroendocrine peptidergic neurons in the pond snail Lymnaea stagnalis, with antisera to the teleostean hormone hypocalcin and mammalian parathyroid hormone.
Immunocytochemical staining with antisera raised against trout hypocalcin, the hypocalcemic hormone of the Stannius corpuscles and against bovine parathyroid hormone (bPTH1-84), revealed a new system of neuroendocrine neurons in the pond snail Lymnaea stagnalis. The neurons are located in small groups or single cells in the visceral, parietal, and pedal ganglia of the central nervous system. The axons of these cells are running to the periphery of the pleuroparietal, visceroparietal, and pleuropedal connections, the dorsopedal commissure, and to several nerves originating in the visceral, parietal, and pedal ganglia. The axons are ending with characteristic axonal distensions in the periphery of these connectives, commissure, and nerves. These regions probably act as neurohaemal areas. The affinity of this neuroendocrine system for both the anti-hypocalcin and anti-PTH sera is another indication for a special relationship between hypocalcin and PTH, which possess some immunological resemblance and similar biological activities, although no similarity in primary structure.
Department of Animal Physiology, Faculty of Science, University of Nijmegen, The Netherlands.
S E
SE
Wendelaar Bonga
F P
FP
Lafeber
G
G
Flik
T
T
Kaneko
P K
PK
Pang
D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006728:Hormones; D007150:Immunohistochemistry; D008195:Lymnaea / Q000378:metabolism*; D008854:Microscopy, Electron; D009474:Neurons / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D009490:Neurosecretory Systems / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000276:immunology / Q000378:metabolism*
D006023:Glycoproteins; D006728:Hormones; D010281:Parathyroid Hormone; C051795:teleocalcin