Identification of semen in stain by determination of the specific activity of L-tartrate-inhibitable acid phosphatase.
For identification of semen in stain the specific activity of L-tartrate-inhibitable acid phosphatase (ACP) was determined. With each stain extract, both enzyme activity and protein concentration were determined, and the specific activity (enzyme activity/protein concentration) was calculated. Seminal stains showed a value of 23.8 +/- 15.2 (mean +/- SD) IU/mg protein, while vaginal fluid stains showed a value of 0.088 +/- 0.049 IU/mg protein. Stains of other body fluids did not show any L-tartrate-inhibitable ACP activity. Furthermore, only eight of 30 plant juice stains showed any levels of L-tartrate-inhibitable ACP, although all plants tested showed ACP activity. As the present method enables us to analyze forensic samples quantitatively, it seems to be useful for forensic practice.
Tamaki
K
K
Dept. of Legal Medicine, Nagoya University School of Medicine, Japan.
Fujisawa
K
K
Okajima
H
H
Sato
K
K
Katsumata
Y
Y
D000135:Acid Phosphatase / Q000032:analysis / Q000037:antagonists & inhibitors*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008297:Male; D011902:Rape / Q000331:legislation & jurisprudence; D012661:Semen / Q000201:enzymology*; D013644:Tartrates / Q000494:pharmacology*
D013644:Tartrates; D000135:Acid Phosphatase; C029768:tartaric acid