Substrate specificity of phenol sulfotransferase from primary cultures of bovine brain microvessel endothelium.
The substrate specificity of the thermostable phenol sulfotransferase (PST) from primary cultures of brain microvessel endothelial cell monolayers was characterized. Selected catecholamines, catecholamine metabolites, and p-nitrophenol at 5, 50, and 500 microM were used as substrates in PST assays of cytosol extracts. Endogenous catecholamines, epinephrine, norepinephrine, and dopamine, exhibited no detectable activity as substrates (500 microM) compared to 500 microM p-nitrophenol as substrate (1.8 pmol/mg/min specific activity) for the PST. In contrast, 500 microM of either deaminated or 3-O-methylated metabolites of catecholamines exhibited intermediate (approximately 1.0 pmol/mg/min specific activity) to low (approximately 0.2 pmol/mg/min specific activity) activity, respectively, as substrates compared to p-nitrophenol as substrate for the PST. Additionally, 500 microM of metabolites of catecholamines that were both deaminated and 3-O-methylated exhibited high activity (greater than 3.0 pmol/mg/min specific activity) as substrates compared to p-nitrophenol as substrate for the PST. Qualitatively similar results were observed at lower substrate concentrations. Therefore, results from this study suggest a potential role for PST as part of the "enzymatic" blood-brain barrier in regulating transendothelial passage of endogeneous catecholamines between the blood and the brain.
Department of Biochemistry, Warsaw Medical School, Poland.
A
A
Baranczyk-Kuzma
K L
KL
Audus
R T
RT
Borchardt
D000818:Animals; D015239:Arylsulfotransferase / Q000378:metabolism*; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply*; D002417:Cattle; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism; D004730:Endothelium, Vascular / Q000201:enzymology*; D004837:Epinephrine / Q000378:metabolism; D008833:Microcirculation / Q000201:enzymology; D009596:Nitrophenols / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D013379:Substrate Specificity
D009596:Nitrophenols; D015239:Arylsulfotransferase; D004298:Dopamine; D009638:Norepinephrine; C024836:4-nitrophenol; D004837:Epinephrine