[The action of membranotropic compounds with various structures on the parameters of the phase transition of dimyristoylphosphatidylcholine].
The effect of substances of different nature on the thermodynamic characteristics of dimyristoylphosphatidylcholine (DMPC) phase transition by the differential scanning microcalorimetry has been studied. The substances disposed in hydrophobic part of membrane--alpha-tocopherol, ubiquinone Q10, ionol and vitamin K3 cause the decrease of enthalpy and cooperativity of phase transition. The substances which have the side hydrocarbon chain (tocopherol and ubiquinone Q10) compared with ones without it (ionol and vitamin K3) and reduced quinones (Q10 and vitamin K3) compared with the oxidized ones have stronger influence on the enthalpy and cooperativity of transition. The inclusion of the local anesthetic dicaine disposed mainly in the zone of polar heads of phospholipids into DMPC membranes decreases the temperature of phase transition considerably and practically does not change the cooperativity. A possibility to use the method of differential scanning microcalorimetry to estimate the localization of membrane tropic substances within lipid bilayer is under discussion.
Sëmin
B K
BK
Bautina
A L
AL
Ivanov
I I
II
D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D003067:Coenzymes; D004134:Dimyristoylphosphatidylcholine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects*; D008563:Membrane Lipids / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology; D014812:Vitamin K / Q000494:pharmacology
D003067:Coenzymes; D008051:Lipid Bilayers; D008563:Membrane Lipids; D014812:Vitamin K; D014451:Ubiquinone; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; C024989:coenzyme Q10; D004134:Dimyristoylphosphatidylcholine
Deĭstvie membranotropnykh soedineniĭ razlichnoĭ struktury na parametry fazovogo perekhoda dimiristoilfosfatidilkholina.