Formic acid as a milder alternative to trifluoroacetic acid and phosphoric acid in two-dimensional peptide mapping.
In reversed-phase chromatography of peptides, formic acid has been shown to successfully replace the stronger traditional trifluoroacetic and phosphoric acids. Detection of non-aromatic peptide at lower wavelength is not impaired and being volatile the acid is easily removed, enabling further studies of the peptides. Also in ion-exchange chromatography (the first step of a two-dimensional approach) formic acid works well with a sulphonic acid ion-exchange resin.
Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand.
D J
DJ
Poll
D R
DR
Harding
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005463:Fluoroacetates; D005561:Formates; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis; D010449:Peptide Mapping / Q000379:methods*; D010756:Phosphoric Acids; D013056:Spectrophotometry, Ultraviolet; D014269:Trifluoroacetic Acid
D005463:Fluoroacetates; D005561:Formates; D007202:Indicators and Reagents; D010756:Phosphoric Acids; D014269:Trifluoroacetic Acid