[The dependence of the clonogenic fibroblast proliferation of human bone marrow on the feeder].
The proliferation of human bone marrow stromal fibroblasts depends on the growth factors. Xenogenic bone marrow cells, previously radiated in a dose of 3000 Gy, are shown to be a source of such factors. The human bone marrow cells contain both stimulators and inhibitors of the fibroblast proliferation. The inhibitory activity of the bone marrow cells increases with their concentration in explants. The optimal culture conditions are developed. The efficiency values of the fibroblast cloning in children and adults are presented and compared.
Vladimirskaia
E B
EB
Aĭmanbetova
A M
AM
Kolosova
L Iu
LIu
Tsuria
V M
VM
D000328:Adult; D000818:Animals; D001707:Biopsy, Needle; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002648:Child; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects; D003114:Colony-Forming Units Assay; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors
D010982:Platelet-Derived Growth Factor
Zavisimost' proliferatsii klonogennykh fibroblastov kostnogo mozga cheloveka ot fidera.