Protein conformation changes as the result of binding to reversed-phase chromatography column materials.
The conformational changes of the protein alpha-chymotrypsinogen which may take place on reversed-phase chromatographic material of differing hydrocarbon chain lengths e.g. C4, C6, C8, C10 and phenyl, have been studied by circular dichroism as a function of 1-propanol concentration and pH of the solvent before and after binding to the reversed-phase material.
Drake
A F
AF
Chemistry Department, Birkbeck College, University of London, U.K.
Fung
M A
MA
Simpson
C F
CF
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002919:Chymotrypsinogen; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation
D002919:Chymotrypsinogen