Differential sensitivity to (dl)-5-methyltetrahydrofolate of normal CFU-GM and HL-60 cells.
We studied the effect of (dl)-5-methyltetrahydrofolate on clonogenic growth of HL-60 cells in comparison with human normal CFU-GM. Seven normal bone marrow samples were tested for CFU-GM assay with or without (dl)-mTHF at concentrations ranging from 1.25 X 10(-4) to 5 X 10(-4) M. (dl)-mTHF only slightly affected CFU-GM formation, while, at the same concentrations, it showed a dose related inhibition of HL-60 colony formation, up to a complete arrest of growth at the doses of 5 X 10(-4) and 1 X 10(-3) M. The same impairment of proliferation was observed in liquid culture. These results are in keeping with reported observations, describing a different membrane system mediating "folate" transport in normal and leukemic cells.
U.O. Ematologia, Universitá degli Studi, Firenze, Italy.
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V
Gattei
P A
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Bernabei
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Ferrini
D001853:Bone Marrow / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003114:Colony-Forming Units Assay; D006131:Growth Inhibitors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D013763:Tetrahydrofolates / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology
D006131:Growth Inhibitors; D013763:Tetrahydrofolates; C005984:5-methyltetrahydrofolate