Organization of the vimentin system and its spatial relationship to the microtubule complex during the division of mammalian cells growing attached and in suspension.
We have used immunofluorescence staining with antibodies that detect vimentin, tubulin and the centrioles to compare the distributions of these respective antigens during the division of several suspension and attached cultured cells. Our observations demonstrate that 1) from distinct interphase organizations in suspension and attached cells, the vimentin system consistently rearranges with the onset of mitosis into a filamentous cage-like structure enclosing the spindle, 2) during cytokinesis, the polar centrosomes relocalize near the midbody in suspension cells while they remain at the pole opposite to it in attached cells, and 3) the vimentin cage is disintegrated and aggregated on each side of the midbody during cytokinesis in lymphoid cells but may be retained in other suspension cells.
Department of Biology, University of Ottawa, Ontario/Canada.
M
M
Paulin-Levasseur
D L
DL
Brown
D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D002502:Centrioles / Q000378:metabolism*; D004173:Dipodomys; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000166:cytology*; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D014404:Tubulin / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*
D014404:Tubulin; D014746:Vimentin