Effect of soman intoxication on the organization of rat brain ribosomes and the translational activity of mRNA in a cell-free system.
The effect of soman on rat brain ribosomes organization and translational activity of mRNA in cell-free system was studied in rats exposed to 1.3 LD50 soman (120 micrograms/kg body weight) and in rats repeatedly injected with 0.4 LD50 soman (35 micrograms/kg). Fifteen minutes after the injection of 1.3 LD50 soman the heavy polyribosomal fraction from rat brain was found to be enriched and translational activity of mRNA was enhanced. In rats administered five injections of 0.4 LD50 soman at 24-h intervals, the low density ribosomes appeared as the predominant fraction whereas the activity of mRNA in all cell-free system was significantly impaired. It is concluded that soman intoxication expresses a stimulatory or inhibitory effect on the processes of protein synthesis in the rat brain, depending on the dose schedule of soman administration.
The Sinisa Stanković Institute for Biological Research, Belgrade, Yugoslavia.
L
L
Sevaljević
B
B
Bosković
M
M
Glibetić
M
M
Tomić
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000648:ultrastructure; D001923:Brain Chemistry / Q000187:drug effects; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D008297:Male; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012270:Ribosomes / Q000187:drug effects*; D012999:Soman / Q000633:toxicity*; D013050:Spectrometry, Fluorescence
D012333:RNA, Messenger; D012999:Soman