Structured modeling and simulation of oxygen transport to hybridoma cell in a suspension culture: theoretical analysis.
The importance of the consideration of micro-anatomical and cytological observation for the oxygen utilization by hybridoma cells are emphasized. Tumor cells have considerably lower content of mitochondria and that cells metabolize much of their carbon source by anaerobic pathway, therefore, consumes much less oxygen. Because of the fact that hybridoma cells are derived from a myeloma cell, which is a type of tumor cell, there may be possibility that the oxygen demand could be closely related to mitochondrial content of the resulting cell. Possible correlation between the optimum DOT and mitochondrial content of the hybridoma cells is hypothesized. By simulating oxygen transport inside of a single hybridoma cells for the cases of three different mitochondrial contents, possible differences in the optimum DOT according to the mitochondrial contents were studied and the results reported.
Kang
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KA
Department of Chemical Engineering, University of California, Davis 95616.
Ryu
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DD
D000818:Animals; D003470:Culture Media; D004058:Diffusion; D006825:Hybridomas / Q000378:metabolism*; D008433:Mathematics; D008928:Mitochondria / Q000378:metabolism; D008962:Models, Theoretical; D010101:Oxygen Consumption
D003470:Culture Media