Evaluation of L6, an anti-carcinoma murine monoclonal antibody, in tumor-bearing nude mice.
L6 is a murine monoclonal antibody (MAb) binding to cells of most human carcinomas, mediating antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity, and inhibiting tumor growth in nude mice [10]. Fab and F(ab')2 fragments of L6, as well as intact MAb, have been evaluated for immunospecific localization in nude mice xenografted with a human lung carcinoma. They were compared with preparations of an isotype-matched control immunoglobulin, P1.17, after labelling with 125I or 131I. L6 Fab fragments prepared from MAb L6 and labelled with 67Ga via desferrioxamine were also tested. The data suggest that MAb L6 may be useful for in vivo detection of human carcinomas.
Oncogen, University of Washington, Seattle.
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E
Lavie
P L
PL
Beaumier
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L
Brodzinsky
K E
KE
Hellström
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I
Hellström
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D005710:Gallium Radioisotopes; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D008657:Metabolic Clearance Rate; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000378:metabolism*; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D005710:Gallium Radioisotopes; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes