Isolation of 11 beta-hydroxylated androgens from testicular vein blood of the mature boar.
The isolation of 11 beta-hydroxyandrostenedione and 11 beta-hydroxytestosterone from testicular vein blood of the mature male domestic pig is described. Blood was collected from veins and arteries on the surface of the testes of mature boars. Steroids were extracted from plasma with SEP-PAK C18 cartridges and recovered with acetonitrile. A separation of steroids was made by high performance liquid chromatography (HPLC) using acetonitrile/water (37/63; v/v), and fractions were collected manually with detection at 254 nm. Preliminary identification was based on comparison with the HPLC retention time of an authentic steroid standard. Final characterization was achieved by means of capillary gas chromatography-mass spectrometry (GC-MS). The findings record the first evidence for the secretion of C19-11-hydroxylated steroids by normal testes in a mammalian species.
Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
J I
JI
Raeside
R L
RL
Renaud
R M
RM
Friendship
D000728:Androgens / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006900:Hydroxylation; D008297:Male; D013552:Swine / Q000097:blood*; D013737:Testis / Q000098:blood supply / Q000378:metabolism*
D000728:Androgens