B cell precursors are present in the thymus during early development.
An in vitro system for transforming immature lymphoid cells present in the thymus at early development has been established. By phenotype analysis of the transformants obtained, we observed that B cell precursors, susceptible to Abelson murine leukemia virus (A-MuLV)- or Harvey murine sarcoma virus (H-MuSV)-induced lymphogenesis, were present at high frequency in the fetal thymus of BALB/c mice. These precursors recolonized alymphoid thymus lobes in vitro, as do T cell precursors. It was further observed that B precursors in the fetal liver were also capable of recolonizing alymphoid thymus lobes and were stored in a thymic environment. These results suggest that stroma cells of the fetal thymus may possess the capacity to support the growth of B precursors. On the other hand, B cell precursors sensitive to the viral transformation were undetectable in the fetal thymus of C57BL/6, although immunohistochemical analysis suggested their presence. However, in the fetal liver of the same strain, B precursors recolonizing alymphoid thymus in vitro were sensitive to the viral transformation. Based on these results, we will discuss both the role and fate of thymic B precursors. In addition, we also obtained T cell lymphomas at different stages of differentiation from the fetal thymus of C57BL/6 infected with A-MuLV or H-MuSV. These data indicate the usefulness of our system in establishing cell lines derived from intrathymic lymphogenesis at early development.
Division of Molecular Immunology, School of Medicine, Chiba University, Japan.
H
H
Kimoto
T
T
Shirasawa
M
M
Taniguchi
T
T
Takemori
D000011:Abelson murine leukemia virus; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte; D001402:B-Lymphocytes / Q000145:classification / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006251:Harvey murine sarcoma virus; D008099:Liver / Q000502:physiology; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000235:genetics / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010641:Phenotype; D013234:Stem Cells / Q000145:classification / Q000378:metabolism / Q000502:physiology*; D013950:Thymus Gland / Q000196:embryology* / Q000276:immunology
D000944:Antigens, Differentiation, B-Lymphocyte