Effects of dietary iron deficiency and tungsten supplementation on 59Fe absorption and gastric retention from 59Fe compounds in rats.
1. In vivo 59Fe absorption from intrinsically labelled Fe-containing fractions of liver and blood were measured in rats by intragastric dosing. All rats were fed on a low-Fe diet for 3 d before dosing in order to standardize the Fe status of the intestinal mucosal cells. 2. An increase in digestion time from 2 to 12 h increased 59Fe absorption (P less than 0.01) from all fractions except ferritin. 3. Fe-deficient rats when compared with essentially Fe-replete rats showed decreased gastric retention for all fractions, but increased 59Fe absorption over 2 h only from ferritin. Ferritin showed several unusual absorption characteristics. 4. Dietary tungsten supplementation of Fe-deficient rats reduced the ferroxidase activity of intestinal mucosal xanthine oxidase. In addition, gastric retention and 59Fe absorption (P less than 0.05) from all fractions were increased.
Department of Applied Biochemistry and Food Science, Nottingham University, School of Agriculture, Sutton Bonington, Loughborough, Leics.
G E
GE
Shears
R J
RJ
Neale
D A
DA
Ledward
D000818:Animals; D004063:Digestion; D005293:Ferritins / Q000378:metabolism; D005746:Gastric Emptying; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000201:enzymology; D007501:Iron / Q000378:metabolism*; D000090463:Iron Deficiencies; D007504:Iron Radioisotopes / Q000378:metabolism; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014414:Tungsten / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D007504:Iron Radioisotopes; D005293:Ferritins; D007501:Iron; D010088:Oxidoreductases; D014969:Xanthine Oxidase; D014414:Tungsten