Labelling monoclonal antibodies with yttrium-90.
Using the cyclic DTPA derivatisation procedure developed by Hnatowich, conditions have been optimised for labelling three tumour-associated monoclonal antibodies with 90Y. High labelling efficiencies (greater than 95%) at modest specific activities (1 mCi/mg) could be routinely obtained. Radiochemical stability of the radiolabelled preparations in vitro was good, but radiolysis resulted in early losses of antibody immunoreactivity.
Department of Nuclear Medicine, St. Bartholomews Hospital, London, UK.
S J
SJ
Mather
D M
DM
Tolley
G W
GW
White
D000911:Antibodies, Monoclonal; D004355:Drug Stability; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D004369:Pentetic Acid; D015021:Yttrium Radioisotopes
D000911:Antibodies, Monoclonal; D015021:Yttrium Radioisotopes; D004369:Pentetic Acid