[3H]5-HT binding in post-mortem human cerebral cortex: methodological considerations.
The effects of different membrane preparations and assay conditions on [3H]5-HT binding to post-mortem human cortical tissue was studied. Optimal binding necessitated thorough removal of endogenous 5-HT and this was achieved either by hypotonic lysis or by preincubation of the membranes at 37 degrees C. Calcium chloride (4 mM) increased specific [3H]5-HT binding. The further addition of ascorbic acid (5.7 mM) or ascorbic acid and clorgyline (10 microM) reduced specific [3H]5-HT binding.
Department of Pharmacology and Clinical Pharmacology, St. George's Hospital Medical School, London, U.K.
S C
SC
Cheetham
R W
RW
Horton
D000328:Adult; D002540:Cerebral Cortex / Q000378:metabolism*; D006801:Humans; D008297:Male; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin