Purification of dolabellanin-C an antineoplastic glycoprotein in the body fluid of a sea hare, Dolabella auricularia.
An antineoplastic factor, dolabellanin C, inducing tumor lysis was purified to apparent homogeneity from the body fluid of the sea hare Dollabella auricularia. Purified dolabellanin C is a glyco-protein of 215 K daltons containing 3 subunits of 70 K daltons. The amino acid sequence of the amino terminal region was also determined. This factor was active even at 0.38 ng protein/ml, but did not lyse normal white or red blood cells.
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
J
J
Kisugi
H
H
Kamiya
M
M
Yamazaki
D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D008974:Mollusca
D000970:Antineoplastic Agents; D006023:Glycoproteins; C060899:dolabellanin C protein, Dollabella auricularia