Glutathione S-transferases and chloroform toxicity in streptozotocin-induced diabetic rats.
The glutathione S-transferase activity in liver and kidney cytosol was significantly decreased in short term diabetes induced with streptozotocin, whereas no decrease in the transferase was observed in phenobarbital-treated diabetic rats. Toxicity of chloroform was potentiated in streptozotocin- or phenobarbital-treated rats. The decrease in liver cytosolic and microsomal glutathione S-transferase activity was observed in long term diabetic rats, and only microsomal transferase activity was restored by insulin treatment. There was no release of glutathione S-transferases into the serum in the diabetic rats, and the transferases were not inhibited by streptozotocin in vitro. These results showed that glutathione S-transferase activity decreased during diabetes, and this decrease may contribute to altering drug metabolism and toxicity in diabetes.
Laboratory of Physiology and Pharmacology, School of Health Sciences, University of the Ryukyus, Okinawa, Japan.
Y
Y
Aniya
Y
Y
Ojiri
R
R
Sunagawa
K
K
Murakami
G
G
Zhenzhong
G
G
Mimura
M
M
Sakanashi
D000410:Alanine Transaminase / Q000097:blood; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002725:Chloroform / Q000633:toxicity*; D003600:Cytosol / Q000201:enzymology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000503:physiopathology*; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D002725:Chloroform; D000815:Aniline Hydroxylase; D005982:Glutathione Transferase; D000410:Alanine Transaminase