Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis.
Monoclonal antibodies (MAb) specific for Chlamydia trachomatis lipopolysaccharide (LPS) and major outer membrane protein (MOMP) were used for immunoelectron microscopy analysis. MAb specific for MOMP showed strong reaction with the chlamydial surface, whereas MAb specific for LPS showed strong association of gold particles with the periphery of the chlamydial body. After fixation of the chlamydia cells, the reactivity was, however, similar to the anti-MOMP reactivity. Enzyme-linked immunosorbent assay showed that MAb specific for LPS could remove LPS from the chlamydial membrane.
Institute of Medical Microbiology, University of Aarhus, Denmark.
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Birkelund
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Lundemose
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Christiansen
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000648:ultrastructure*; D002692:Chlamydia trachomatis / Q000032:analysis / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000032:analysis*; D008854:Microscopy, Electron
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides