Production of interleukin 6 and its relation to the macrophage differentiation of mouse myeloid leukemia cells (M1) treated with differentiation-inducing factor and 1 alpha,25-dihydroxyvitamin D3.
We have studied the production of interleukin 6 (IL-6) and its relation to the macrophage differentiation in murine myeloid leukemia cells (M1). As has been reported, differentiation-inducing factor (D-factor), 1 alpha, 25-dihydroxyvitamin D3 [1 alpha, 25(OH)2D3], and recombinant IL-6 similarly induced differentiation of M1 cells into macrophages. The three compounds also induced mRNA expression of IL-6 in M1 cells. M1 cells treated with D-factor or 1 alpha, 25(OH)2D3 produced biologically active IL-6, but the amounts of IL-6 secreted into culture media did not appear to be enough to induce differentiation of M1 cells. Furthermore, simultaneous addition of anti-IL-6 antibody did not suppress the differentiation of M1 cells induced by D-factor or 1 alpha, 25(OH)2D3. These results show that IL-6 production is an essential property associated with the macrophage differentiation of M1 cells, but it may not be responsible for the D-factor- and 1 alpha, 25(OH)2D3-induced differentiation.
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
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Miyaura
C H
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Jin
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Yamaguchi
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Tomida
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Hozumi
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Matsuda
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Hirano
T
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Kishimoto
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Suda
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002454:Cell Differentiation; D005786:Gene Expression Regulation / Q000187:drug effects; D006131:Growth Inhibitors; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D053605:Leukemia Inhibitory Factor; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology*; D008222:Lymphokines / Q000494:pharmacology*; D008264:Macrophages / Q000473:pathology*; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D006131:Growth Inhibitors; D015850:Interleukin-6; D007378:Interleukins; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D012333:RNA, Messenger; D002117:Calcitriol