Capillary gas chromatography and tandem mass spectrometry of paf-acether and analogs: absence of 1-O-alkyl-2-propionyl-sn-glycero-3-phosphocholine in human polymorphonuclear neutrophils.
Fast atom bombardment-tandem mass spectrometry was used to identify molecular species of paf-acether (paf) produced by human polymorphonuclear neutrophils. Using this biological material, normal phase high performance liquid chromatography was necessary prior to the fast atom bombardment-tandem mass spectrometry step. Gas liquid chromatography/electron capture detection after hydrolysis with phospholipase C and conversion to heptafluorobutyrate derivatives was used to confirm the results. The results indicated the presence of mainly 1-O-hexadecyl/octadecyl-2-acetyl-sn-glycero-3-phosphocholine, acyl analogs of paf and only trace amounts of other alkyl analogs of paf. We did not detect the 2-propionyl analog of paf. Moreover, supplementation of human polymorphonuclear neutrophils with sodium propionate did not result in formation of the 2-propionyl analog of paf.
Laboratoire de Chimie Analytique II, S.A.M.M., Faculté de Pharmacie, Chatenay Malabry, France.
M J
MJ
Bossant
R
R
Farinotti
F
F
De Maack
G
G
Mahuzier
J
J
Benveniste
E
E
Ninio
D004583:Electrons; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000097:blood; D010738:Type C Phospholipases
C060636:1-O-alkyl-2-propionyl-sn-glycero-3-phosphocholine; D010972:Platelet Activating Factor; D010738:Type C Phospholipases