A single-amino-acid substitution eliminates the stringent carbohydrate requirement for intracellular transport of a viral glycoprotein.
In this report, we have investigated the contribution of primary sequence to the carbohydrate requirement for intracellular transport of two closely related glycoproteins, the G proteins of the San Juan and Orsay strains of vesicular stomatitis virus. We used site-directed mutagenesis of the coding sequence to eliminate the two consensus sites for glycosylation in the Orsay G protein. Whereas the nonglycosylated San Juan G protein required at least one of its two asparagine-linked oligosaccharides for transport to the plasma membrane at 37 degrees C, a fraction of the Orsay G protein was transported without carbohydrate. Of the 10 amino acid differences between these two proteins, residue 172 (tyrosine in San Juan, aspartic acid in Orsay) played the major role in determining the stringency for the carbohydrate requirement. The rates at which the glycosylated and nonglycosylated Orsay G proteins were transported to the cell surface were the same, although a smaller fraction of the nonglycosylated protein was transported. These results suggest that the carbohydrate does not promote intracellular transport directly but influences a polypeptide folding or oligomerization step which is critical for transport.
Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, California 92138.
A M
AM
Pitta
J K
JK
Rose
C E
CE
Machamer
D000596:Amino Acids / Q000032:analysis; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D006031:Glycosylation; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009154:Mutation; D011487:Protein Conformation; D014415:Tunicamycin / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D000596:Amino Acids; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014415:Tunicamycin