[Interspecies variability in the organization of repeated sequences of the genus Hordeum].
Seven barley species have been compared for organization of repeated sequences. Quantitative variation of repeated DNA fractions is demonstrated, though the total amount of sequences (reassociation up to Cot=10) in most cases does not vary. The repeats are divided into four groups by the mode of interspecific variability, with the help of dot and blot hybridization of the genomes under study with cloned highly repeated sequences of Hordeum vulgare. The first group contains the pHv7161 family of the most conservative sequences. The second group comprises moderately changing repeats. The third group includes highly variable Hind III repeats of Hordeum genomes, and the fourth group is represented by pHv7191 family of repeats that are highly amplified in H. vulgare genome. Comparative analysis of content and organization of highly repeated sequences in genome helps to clarify phylogenetic relationships in the genus and can be used for prediction of successfullness of interspecific hybridization.
Salina
E A
EA
Timofeeva
L L
LL
Vershinin
A V
AV
D004247:DNA / Q000235:genetics; D002523:Edible Grain / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004247:DNA
Mezhvidovaia izmenchivost' v organizatsii povtoriaiushchikhsia posledovatel'nosteĭ roda Hordeum.