Steady-state fluorescence anisotropy and multifrequency phase fluorometry on oxidized phosphatidylcholine vesicles.
Multilamellar liposomes, from mixtures of unoxidized (control) and singlet oxygen oxidized phosphatidylcholine, were studied by steady-state fluorescence anisotropy and multifrequency phase fluorometry using 1,6-diphenyl-1,3,5-hexatriene (DPH) as fluorescent probe. Lifetime fluorescence decay of the DPH-labeled liposomes was analyzed either by a model of discrete exponential components and a model that assumes a continuous distribution of lifetime values. Increasing the oxidized phosphatidylcholine content in the liposomes, an increase of the membrane interior polarity and a decrease of membrane fluidity occurs which can be related to the hydroperoxide-lipids and double bonds conjugation, respectively.
Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università di Ancona, Italy.
F
F
Tanfani
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G
Curatola
E
E
Bertoli
D004161:Diphenylhexatriene; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide; D015227:Lipid Peroxidation; D008081:Liposomes; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D005456:Fluorescent Dyes; D008081:Liposomes; D010713:Phosphatidylcholines; D004161:Diphenylhexatriene; D006861:Hydrogen Peroxide