The effects of vaccination with tissue culture-derived viral vaccines on detection of antibodies to equine arteritis virus by enzyme-linked immunosorbent assay (ELISA).
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of serum antibodies to equine arteritis virus (EAV). Results from this assay produced a good correlation with results from virus neutralisation tests in horses which had not been regularly vaccinated with commercially available mammalian tissue culture-derived viral vaccines. Vaccination of some horses with tissue culture-derived vaccines induced the formation of antibodies to bovine serum. These antibodies reacted with the bovine protein contaminants in the EAV ELISA antigen, producing false-positive results. Non-viral protein contaminants were found to be closely associated with EAV in that they co-purified with the virus during gradient centrifugation.
Equine Virology Unit, Animal Health Trust, Newmarket, Suffolk, Gt. Britain.
R F
RF
Cook
S J
SJ
Gann
J A
JA
Mumford
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006736:Horses / Q000276:immunology*; D009500:Neutralization Tests; D014035:Togaviridae / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D014765:Viral Vaccines