Direct preparations from chorionic villi--relationship between villous morphology and mitotic index.
It has been postulated that chorionic villi with abundant sprouts have a higher mitotic index and are therefore preferable for obtaining direct chromosome preparations from chorionic villus samples. This theory was tested by correlating villous morphology with mitotic index. Surprisingly, no statistically significant relationship was found. Choice of culture medium, however, was found to be important, with serum-free RPMI yielding a higher mitotic index than 40 per cent FCS in MEM. We conclude that villous morphology, as assessed in this study, is not a major factor in determining the success of direct chromosome preparations.
Romagnano
A
A
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, MO 63110.
Featherstone
T
T
Sun
L
L
Crane
J P
JP
Cheung
S W
SW
D002478:Cells, Cultured; D015193:Chorionic Villi Sampling; D003470:Culture Media; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods; D008938:Mitosis; D008940:Mitotic Index; D011247:Pregnancy
D003470:Culture Media