Effects of dietary iron deficiency and tungsten supplementation on 59Fe absorption and gastric retention from 59Fe compounds in rats.
1. In vivo 59Fe absorption from intrinsically labelled Fe-containing fractions of liver and blood were measured in rats by intragastric dosing. All rats were fed on a low-Fe diet for 3 d before dosing in order to standardize the Fe status of the intestinal mucosal cells. 2. An increase in digestion time from 2 to 12 h increased 59Fe absorption (P less than 0.01) from all fractions except ferritin. 3. Fe-deficient rats when compared with essentially Fe-replete rats showed decreased gastric retention for all fractions, but increased 59Fe absorption over 2 h only from ferritin. Ferritin showed several unusual absorption characteristics. 4. Dietary tungsten supplementation of Fe-deficient rats reduced the ferroxidase activity of intestinal mucosal xanthine oxidase. In addition, gastric retention and 59Fe absorption (P less than 0.05) from all fractions were increased.
Shears
G E
GE
Department of Applied Biochemistry and Food Science, Nottingham University, School of Agriculture, Sutton Bonington, Loughborough, Leics.
Neale
R J
RJ
Ledward
D A
DA
D000818:Animals; D004063:Digestion; D005293:Ferritins / Q000378:metabolism; D005746:Gastric Emptying; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000201:enzymology; D007501:Iron / Q000378:metabolism*; D000090463:Iron Deficiencies; D007504:Iron Radioisotopes / Q000378:metabolism; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014414:Tungsten / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D007504:Iron Radioisotopes; D005293:Ferritins; D007501:Iron; D010088:Oxidoreductases; D014969:Xanthine Oxidase; D014414:Tungsten