Elevated plasma interleukin-1 levels in humans following ultraviolet light therapy for psoriasis.
Interleukin-1 (IL-1) is a potent cytokine with a wide range of biologic activities including induction of several acute phase responses. Ultraviolet radiation (UVR) is widely used as a therapeutic modality to treat many chronic skin diseases, including psoriasis. In the present study, we investigated whether ultraviolet B (UVB) radiation induced circulating IL-1 in the plasma of patients undergoing chronic UVB therapy. In order to remove plasma proteins which inhibit IL-1-induced T-cell proliferation, each plasma sample was chromatographed and each fraction was assayed for IL-1 activity. There was no detectable IL-1 before and 1 h after UVB radiation; IL-1 appeared 4 h after treatment and was absent after 24 h. Plasma IL-1 was neutralized by antibodies to recombinant human IL-1 beta and alpha. The anti-IL-1 alpha, but not anti-IL-1 beta, antibodies partially neutralized the IL-1 activity present in a keratinocyte cell line supernate. These results demonstrate that UVB therapy induces circulating IL-1 and that this IL-1 may originate from both keratinocyte and non-keratinocyte sources.
Department of Dermatology, New England Medical Center, Boston, MA 02111.
N
N
Konnikov
S H
SH
Pincus
C A
CA
Dinarello
D000906:Antibodies / Q000276:immunology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D011565:Psoriasis / Q000097:blood / Q000532:radiotherapy*; D011994:Recombinant Proteins / Q000276:immunology; D014467:Ultraviolet Therapy
D000906:Antibodies; D007375:Interleukin-1; D011994:Recombinant Proteins