The role of gut micro-organisms in the metabolism of deoxynivalenol administered to rats.
1. Oral administration of deoxynivalenol (DON) to control rats resulted in the appearance of a de-epoxy metabolite in urine and faeces. 2. When DON was administered to rats treated with antibiotics to deplete their gut microflora there was very little excretion of radioactivity as the de-epoxy metabolite in faeces or urine. 3. Incubation of DON with a strictly anaerobic preparation of gut contents resulted in the progressive appearance of de-epoxy DON during a 24 h incubation period. 4. Incubation of DON with liver homogenate did not result in the appearance of the de-epoxy DON metabolite. 5. These results indicate that the presence of de-epoxy DON in rat excreta, following the oral administration of DON, is the result of metabolism by micro-organisms in the gut.
Worrell
N R
NR
BIBRA, Carshalton, Surrey, UK.
Mallett
A K
AK
Cook
W M
WM
Baldwin
N C
NC
Shepherd
M J
MJ
D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D005766:Gastrointestinal Contents; D066298:In Vitro Techniques; D007422:Intestines / Q000382:microbiology*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000378:metabolism*; D014255:Trichothecenes / Q000008:administration & dosage / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D012717:Sesquiterpenes; D014255:Trichothecenes; C048238:deepoxy-deoxynivalenol; C007262:deoxynivalenol