New findings on the purification and characterization of an anti-bothropic factor from Didelphis marsupialis (opossum) serum.
We have used DEAE-Sephacel and Sephacryl S-200 to separate protein fractions from Didelphis marsupialis serum capable of protecting mice from the lethal effect of Bothrops jararaca venom. The fractions separated were homogeneous by conventional electrophoresis using cellulose acetate and polyacrylamide; however, they were heterogeneous on PAGE-SDS, showing similar electrophoretic patterns with or without mercaptoethanol. The protein bands obtained were glycoproteins with a molecular weight of 42,000 to 58,000 Daltons.
Departamento de Fisiologia e Farmacodinâmica, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
J
J
Perales
R
R
Muños
S
S
Graterol
O
O
Oviedo
H
H
Moussatché
D000818:Animals; D000997:Antivenins / Q000276:immunology / Q000302:isolation & purification*; D003435:Crotalid Venoms / Q000276:immunology* / Q000506:poisoning; D004588:Electrophoresis, Cellulose Acetate; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D009893:Opossums / Q000097:blood*
D000997:Antivenins; D003435:Crotalid Venoms