The interactions of factors X and IX with phospholipid.
The interactions of human factors X and IX with phospholipid were studied with an ELISA system. In the presence of calcium ions, factor X bound to phosphatidylserine with an affinity of 1.56 x 10(10) M-1 and to phosphatidylcholine with an affinity of 5.6 x 10(9) M-1. In the presence of calcium ions, factor IX bound to phosphatidylserine with an affinity of 8.4 x 10(8) M-1 and no binding to phosphatidylcholine was observed. No competition of factors X and IX for phosphatidylserine binding sites was observed.
Protein Purification Research Cutter Biological Miles Inc., Berkeley, CA 94710.
J W
JW
Bloom
D001667:Binding, Competitive; D002118:Calcium / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005164:Factor IX / Q000378:metabolism*; D005170:Factor X / Q000378:metabolism*; D006801:Humans; D010713:Phosphatidylcholines / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*
D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D010743:Phospholipids; D005164:Factor IX; D005170:Factor X; D002118:Calcium