Microbial transformations of steroids--III. Transformation of progesterone by Sepedonium ampullosporum.
The 16 alpha-steroid hydroxylating fungus Sepedonium ampullosporum (CMI strain 203 033) transformed progesterone into 16 alpha-hydroxyprogesterone and four other major metabolites which have not been reported previously for this organism, 6 beta-hydroxyprogesterone, 17 alpha-hydroxyprogesterone, 16 alpha-hydroxyandrostenedione and 16-oxotestosterone (16-ketotestosterone). Among the minor metabolites we have been able to identify 15 alpha-hydroxyprogesterone. This compound has not been reported for S. ampullosporum. The conditions used for transformation had comparatively little effect on the relative proportions of products formed, 16 alpha-hydroxyprogesterone always being the predominant metabolite, but had a major effect on the total yields of metabolites isolatable. These findings suggest that one or more constitutive enzyme systems were responsible for the transformations.
Smith
K E
KE
School of Biological Sciences, Queen Mary College, London, U.K.
White
K A
KA
Kirk
D N
DN
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D003904:Mitosporic Fungi / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D013997:Time Factors
D011374:Progesterone