Intracellular targetting signals of polymeric immunoglobulin receptors are highly conserved between species.
A rat liver cDNA library, constructed in the plasmid expression vector pUEX, was immunoscreened using a rabbit polyclonal antiserum raised against rat liver Golgi membrane proteins. A sub-set of isolated clones were shown to encode the rat polymeric immunoglobulin receptor (pIgR). DNA sequence analysis of these clones provided the complete coding sequence of rat pIgR. Subsequent alignment of rat, rabbit and human predicted amino acid sequences demonstrated that the greatest degree of homology between the three pIgRs lies in their cytoplasmic tails; a region previously shown to be important for correct targetting and trancytosis of rabbit pIgR [(1984) Nature 308, 37-43].
European Molecular Biology Laboratory, Heidelberg, FRG.
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G
Banting
B
B
Brake
P
P
Braghetta
J P
JP
Luzio
K K
KK
Stanley
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D006056:Golgi Apparatus / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000235:genetics*; D008099:Liver / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D021382:Protein Sorting Signals / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D011971:Receptors, Immunologic / Q000032:analysis / Q000235:genetics*; D012635:Secretory Component / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D007074:Immunoglobulin G; D008565:Membrane Proteins; D021382:Protein Sorting Signals; D011971:Receptors, Immunologic; D012635:Secretory Component; C044411:polymeric IgG; D004247:DNA