[Analysis with immunoreactive CCKs and bioactive CCKs in rat duodenum and brain].
We extracted immunoreactive CCKs from rat duodenum and brain. We fractionated these samples by gel-filtration and analyzed with immunoreactivity and bioactivity of each fraction. 1) Elution profile of CCKs in duodenum and brain was well corresponding to that of bioactive CCKs. 2) Contents of CCKs determined by both RIA and Bioassay in each fraction were well correlated with each other. 3) Apparent molecular weights of CCKs of duodenum calibrated in column chromatography were approximately 2200, 1000 and 800. 4) Apparent molecular weight of CCK of brain seemed to be 1000.
Ohnishi
S
S
Adachi
H
H
Noguchi
M
M
Sato
S
S
Honda
T
T
Aoki
E
E
Katsushima
S
S
Konishi
J
J
Tateishi
K
K
Hamaoka
T
T
D000818:Animals; D001923:Brain Chemistry; D002766:Cholecystokinin / Q000276:immunology / Q000378:metabolism*; D004386:Duodenum / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D002766:Cholecystokinin