Measuring creatine kinase MB isoenzyme in a maintenance hemodialysis population: chemiluminometric immunoassay and electrophoresis compared.
In an effort to clarify the issue of potentially false increases in creatine kinase (EC 2.7.3.2) MB isoenzyme (CK-MB) in uremia, we evaluated the CK profile of 84 persons undergoing chronic maintenance hemodialysis. We compared the performance of a new commercial two-site chemiluminometric immunoassay of CK-MB (Magic Lite; Ciba Corning Diagnostics) with that of electrophoresis on agarose gel (Cardio Trak-CK; Corning Medical). Results of the new chemiluminometric immunoassay for samples from hemodialysis patients correlated well with those of the electrophoretic method (r = 0.86, P less than 0.001), showing that neither substances in the serum of uremic patients nor CK-MM isoenzyme give false-positive increases in CK-MB isoenzyme. Our evidence suggests that the chemiluminometric method may be more specific than is electrophoresis in establishing absolute CK-MB values in the diagnosis of suspected myocardial injury in this population.
Department of Pathology, Pacific Presbyterian Medical Center, San Francisco, CA 94120.
B P
BP
Latner
J S
JS
Skale
W
W
Burns
D003402:Creatine Kinase / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D005189:False Positive Reactions; D006801:Humans; D007118:Immunoassay; D007527:Isoenzymes; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis*; D011933:Reagent Kits, Diagnostic; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood* / Q000628:therapy
D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D003402:Creatine Kinase