Low-temperature and conventional scanning electron microscopy of human urothelial neoplasms.
The appearance of neoplastic human urothelium viewed by low-temperature scanning electron microscopy (LTSEM) and conventional scanning electron microscopy (CSEM) was compared. Fixed, dehydrated neoplastic cells viewed by CSEM had well-defined, often raised cell junctions; no intercellular gaps; and varying degrees of pleomorphic surface microvilli. The frozen hydrated material viewed by LTSEM, however, was quite different. The cells had a flat or dimpled surface, but no microvilli. There were labyrinthine lateral processes which interdigitated with those of adjacent cells and outlined large intercellular gaps. The process of fixation and dehydration will inevitably distort cell contours and on theoretical grounds, the images of frozen hydrated material should more closely resemble the in vivo appearance.
Hopkins
D M
DM
Institute of Environmental and Biological Sciences, University of Lancaster, U.K.
Morris
J A
JA
Oates
K
K
Huddart
H
H
Staff
W G
WG
D000368:Aged; D000369:Aged, 80 and over; D002295:Carcinoma, Transitional Cell / Q000648:ultrastructure*; D003080:Cold Temperature; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D014021:Tissue Preservation / Q000379:methods; D001749:Urinary Bladder Neoplasms / Q000648:ultrastructure*