Direct effects of furosemide and amiloride on the perfused and ischaemic rat heart.
The present study was undertaken in order to assess direct effects of furosemide and furosemide plus amiloride upon the perfused and ischaemic isolated rat heart. Furosemide in concentrations ranging between 4-400 mg/l in the perfusate increased coronary flow in a concentration dependent manner. There was no evidence for a negative inotropic effect of furosemide. However, very high doses of furosemide (400 mg/l) decreased the post-ischaemic values of left ventricular developed pressure, coronary flow rate, adenosine triphosphate, creatine phosphate and potassium, and increased the myocardial content of calcium and sodium. Furosemide 4 mg/l and 40 mg/l had no effect on post-ischaemic parameters compared to the control group except that furosemide 40 mg/l increased the recovery of coronary flow. Although amiloride 13.3 mg/l alone did not affect post-ischaemic recovery, the addition of this dose to furosemide 400 mg/l improved the post-ischaemic recovery of left ventricular developed pressure, coronary flow rate and adenosine triphosphate. The myocardial content of magnesium and potassium was higher indicating protection of amiloride by its magnesium- and potassium-sparing properties opposing ischaemic losses aggravated by the exposure to furosemide.
Department of Pharmacology and Toxicology, Faculty of Medicine, University of Trondheim, Norway.
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Borchgrevink
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Jynge
D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects; D003326:Coronary Circulation / Q000187:drug effects*; D003327:Coronary Disease / Q000378:metabolism*; D004573:Electrolytes / Q000378:metabolism; D004734:Energy Metabolism; D006339:Heart Rate / Q000187:drug effects; D006352:Heart Ventricles / Q000378:metabolism; D008297:Male; D010710:Phosphates / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004573:Electrolytes; D010710:Phosphates