The effect of cryopreservation on ciliary beat frequency of human respiratory epithelium.
The effect of cryopreservation on ciliary activity of human nasal respiratory epithelium was evaluated. Samples were cryopreserved in a solution containing nutrient medium, 10% fetal calf serum, and two different concentrations (10 or 20%) of dimethyl sulfoxide and stored in liquid nitrogen at -196 degrees C for 2 weeks. Ciliary beat frequencies (CBF) of the samples before and after cryopreservation were compared. Mean CBF values did not differ significantly with both concentrations of dimethyl sulfoxide. The mean intrasample coefficient of variation of the CBF decreased significantly after cryopreservation. After thawing, CBF remained unchanged for at least 4 hr. It is concluded that normal ciliated epithelial cells can be frozen and stored in liquid nitrogen at 196 degrees C while maintaining their CBF.
Department of Thoracic Medicine, Royal Free Hospital, Hampstead, London, United Kingdom.
G
G
Di Benedetto
J
J
Gill
M T
MT
Lopez-Vidriero
S W
SW
Clarke
D000328:Adult; D002923:Cilia / Q000502:physiology; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D005615:Freezing; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009297:Nasal Mucosa / Q000502:physiology / Q000648:ultrastructure; D014021:Tissue Preservation