Antipeptide antibodies toward the extracellular domain of insulin receptor beta-subunit.
In order to investigate structure and function of beta-subunit extracellular portion, four polyclonal antibodies (AP1, AP2, AP3 and AP4) toward peptides comprised in this region were generated. None of them recognizes native human and rat insulin receptor both in vitro and in whole cells. Two antibodies, AP1 and AP2, immunoprecipitate isolated (DTT-reduced) human beta-subunits and bind to human IM-9 cell after alpha-subunit tryptic cleavage. Only AP1 recognizes rat beta-subunit both in vitro and in trypsin treated rat FAD cells. These findings suggest that: (i) the extracellular portion of the insulin receptor beta-subunit is partially covered by the alpha-subunit in human and rat native insulin receptors; (ii) human and rat beta-subunit extracellular domains are different, at least in the amino acid sequence corresponding to residues 785-796 of the human insulin receptor.
Dpt. of Internal Medicine, University of Genova, Italy.
A
A
Pessino
R
R
Gherzi
G
G
Damiani
R
R
Longhi
L
L
Adezati
R
R
Cordera
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011233:Precipitin Tests; D011487:Protein Conformation; D051381:Rats; D011972:Receptor, Insulin / Q000276:immunology* / Q000648:ultrastructure; D013045:Species Specificity
D000906:Antibodies; D046911:Macromolecular Substances; D010455:Peptides; D011972:Receptor, Insulin