Biochemical and biological profile of a new enzyme preparation from Antarctic krill (E. superba) suitable for debridement of ulcerative lesions.
A protease extract from Antarctic krill (E. superba) intended as a new enzymatic debrider for necrotic ulcers has been characterized by sodium dodecyl sulphate polyacrylamide gradient gel electrophoresis and fast protein liquid chromatography. The predominant enzymes in the preparation represent trypsin-like activity associated with three serine proteinases. In addition two carboxypeptidases A and B are present as cooperative enzymes for a more complete breakdown of complex proteinaceous substrates. Biological studies on a well-defined substrate (fibrin) originating from leg ulcers, demonstrated more effective degradation by krill enzymes than bovine trypsin, a common component in marketed enzymatic debriders. These findings support previously in vitro/in vivo studies in an animal model (rat) using excised rat skin as "necrotic" tissue.
Anheller
J E
JE
Pharmacia AB, Uppsala, Sweden.
Hellgren
L
L
Karlstam
B
B
Vincent
J
J
D000818:Animals; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000378:metabolism; D043422:Carboxypeptidases A; D002851:Chromatography, High Pressure Liquid; D003445:Crustacea / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D057487:Enzyme Therapy; D004798:Enzymes / Q000032:analysis* / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D007871:Leg Ulcer / Q000188:drug therapy*; D014357:Trypsin / Q000378:metabolism
D004798:Enzymes; D005337:Fibrin; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D043424:Carboxypeptidase B; D014357:Trypsin