Differential effects of phospholipase inhibitors in long-term potentiation in the rat hippocampal mossy fiber synapses and Schaffer/commissural synapses.
Bath application of the inhibitors of phospholipases, nordihydroguaiaretic acid (NDGA) and p-bromo-phenacyl bromide (BPB), to the rat hippocampal slices suppressed long-term potentiation (LTP) in Schaffer/commissural-CA1 pyramidal synapses. On the other hand, neither of the two inhibitors suppressed LTP in mossy fiber-CA3 pyramidal cell synapses. BPB did not suppress phosphatidylinositol-specific phospholipase C (PI-PLC) activity of the slices. These results suggested that the mechanisms of LTP were quite different in the CA1 and CA3 subfields of rat hippocampus: in CA1, the involvement of an arachidonate metabolism was strongly suggested, whereas in CA3, an arachidonic acid cascade may not be necessary for LTP.
Okada
D
D
National Institute for Physiological Sciences, Department of Cellular Physiology, Okazaki, Japan.
Yamagishi
S
S
Sugiyama
H
H
D000098:Acetophenones / Q000494:pharmacology*; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D066298:In Vitro Techniques; D009637:Masoprocol / Q000494:pharmacology*; D010740:Phospholipases / Q000502:physiology*; D051381:Rats
D000098:Acetophenones; D002396:Catechols; D004791:Enzyme Inhibitors; D009637:Masoprocol; D010740:Phospholipases; C006821:4-bromophenacyl bromide