CD and small-angle x-ray scattering of silk fibroin in solution.
We investigated the structure of silk fibroin dissolved in water and in water-organic solvent mixtures by CD and small-angle x-ray scattering (SAXS). CD spectra indicated a disordered secondary structure in water and a beta-sheet conformation in aqueous organic solvents, such as methanol, dioxane, and trifluoroethanol (in trifluoroethanol a transient form evolving toward beta-sheet conformation was seen just after dissolution). The SAXS technique indicated the presence of fibroin particles of lamellar shape. The molecular weight was 188,000 daltons in water and 302,000 daltons in aqueous methanol.
M
M
Canetti
A
A
Seves
F
F
Secundo
G
G
Vecchio
D000818:Animals; D012831:Bombyx; D002942:Circular Dichroism; D005349:Fibroins; D000432:Methanol; D008970:Molecular Weight; D011487:Protein Conformation; D012542:Scattering, Radiation; D014867:Water
D014867:Water; D005349:Fibroins; D000432:Methanol