Transport and accumulation of 2-deoxy-D-glucose in wild-type and hexokinase-deficient cultured Chinese-hamster ovary (CHO) cells.
Hexokinase-deficient mutants and wild-type Chinese-hamster ovary cells have been used to investigate the role of hexokinase in uptake and accumulation of 2-D-deoxyglucose (2-dGlc). The evidence for a specific sugar transport system in both types of cells is that there is similar saturable phloretin-sensitive uptake of 2-dGlc and 3-O-methyl-D-glucose (3-OMG) in both types of cell. In wild-type cells, 2-dGlc is accumulated to a tissue:medium ratio of 10- and in the mutant only 3-fold; 3-OMG is not accumulated by either mutant or wild-type cells. The evidence that hexokinase affects the membrane transport process is that the rate of exit of free 2-dGlc from wild-type cells is 5-fold less than from mutant cells, whereas there is no difference in the rate of loss of 3-OMG between mutant and wild-type cells.
Department of Biochemistry, United Medical School of Guy's, St. Thomas' Hospital London, U.K.
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Morgan
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Naftalin
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D019325:3-O-Methylglucose; D000818:Animals; D001693:Biological Transport, Active; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003837:Deoxy Sugars / Q000493:pharmacokinetics*; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D005260:Female; D006593:Hexokinase / Q000502:physiology*; D008757:Methylglucosides / Q000378:metabolism; D010053:Ovary / Q000378:metabolism
D003837:Deoxy Sugars; D008757:Methylglucosides; D019325:3-O-Methylglucose; D003847:Deoxyglucose; D006593:Hexokinase