Flow cytometric analysis of effects of 1,3-dinitrobenzene on rat spermatogenesis.
Exposure of 100-d old rats to 1,3-dinitrobenzene (m-DNB) at dosages up to 48 mg/kg resulted in disruption of spermatogenesis as measured by flow cytometry (FCM) of acridine orange-stained sperm and testis cells. One day (d 1) after a single exposure to 48 mg/kg m-DNB, FCM measurements of caput epididymal fluid cells demonstrated the presence of testicular germinal epithelial cells apparently sloughed off into the epididymis. Also, at d 1 after the same exposure, a decrease in pachytene spermatocytes was observed. By d 16 after exposure to 32 or 48 mg/kg, testicular damage was evidenced by an alteration of cell type ratios in FCM-analyzed populations of testicular cells. Extensive recovery of cell type ratios occurred by d 32. At d 16, dosages of 32 and 48 mg/kg caused alterations of sperm chromatin structure as determined by the flow cytometric sperm chromatin structure assay (SCSA); 48 mg/kg caused alterations at both d 16 and d 32. Exposure to m-DNB caused a dose response increase in percent sperm head morphology abnormalities (%ABN) assessed in cauda epididymal and vas sperm. A slightly higher correlation existed between dose and SCSA alpha t values (d 16, .78; p less than .01) than between dose and %ABN (d 16, .70; p less than .01). Also, a higher correlation existed between standard deviation of alpha t (SD alpha t) values and %ABN (.97; p less than .01) than between dose and %ABN (.70; p less than .01). This study demonstrated rapid and unique FCM procedures originally derived for reproductive toxicology studies in mice to be equally useful for studies in rats.
Evenson
D P
DP
Olson Biochemistry Laboratories, South Dakota State University, Brookings 57007.
Janca
F C
FC
Jost
L K
LK
Baer
R K
RK
Karabinus
D S
DS
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002843:Chromatin / Q000235:genetics; D004136:Dinitrobenzenes / Q000633:toxicity*; D004171:Diploidy; D004822:Epididymis / Q000187:drug effects; D005434:Flow Cytometry; D006238:Haploidy; D008297:Male; D009578:Nitrobenzenes / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012260:Ribonucleases; D013010:Sonication; D013087:Spermatids / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects; D014649:Vas Deferens / Q000187:drug effects
D002843:Chromatin; D004136:Dinitrobenzenes; D009578:Nitrobenzenes; C017906:3-dinitrobenzene; D012260:Ribonucleases