Antioxidant activity of L-carnosine, a natural histidine-containing dipeptide in crystalline lens.
Lipid peroxidation was shown to be an initiatory cause of cataract development in some cases. It has been established that injection into the vitreous body of the rabbit eye of a suspension of liposomes prepared from phospholipids containing lipid peroxidation products induces the development of posterior subcapsular cataract. Such modelling of cataract is based on a type of clouding of the crystalline lens similar to that observed in cataract resulting from diffusion of toxic lipid peroxidation products from the retina to the lens through the vitreous body on degeneration of the photoreceptors. Saturated liposomes (prepared from dipalmitoylphosphatidylcholine) did not cause clouding of the lens, which demonstrated the peroxide mechanism of the genesis of this form of cataract. Clouding of the lens was accompanied by accumulation of fluorescing lipid peroxidation products in the vitreous body, aqueous humor and the lens and also by a fall in the concentration of reduced glutathione in the lens. The ability of L-carnosine (beta-alanyl-L-histidine) to interact directly with lipid peroxidation products suggested its anticataract properties. The effect of L-carnosine on inhibiting or reversing the formation of cataract induced by the administration of lipid peroxidation products was discovered. This phenomenon appeared to be related with normalization of the peroxide metabolism parameters in the crystalline lens. In view of the data, an aqueous solution of L-carnosine is physiologically acceptable in effective nonsurgical treatment of cataracts.
Department of Biochemistry, Moscow Helmholtz Research Institute of Eye Diseases, Moscow State University, U.S.S.R.
M A
MA
Babizhayev
D000818:Animals; D000975:Antioxidants; D002336:Carnosine / Q000493:pharmacokinetics / Q000494:pharmacology*; D002386:Cataract / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D004151:Dipeptides / Q000494:pharmacology*; D006639:Histidine / Q000378:metabolism; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation; D011817:Rabbits
D000975:Antioxidants; D004151:Dipeptides; D006639:Histidine; D002336:Carnosine