Low uptake of [3H]2-deoxy-D-glucose by cultured rat Schwann cells.
[3H]2-Deoxy-D-glucose (2-DG) was used to investigate the glucose uptake in cultured rat Schwann cells from postnatal Sprague-Dawley rat sciatic nerves. The glucose uptake of Schwann cells slightly increased in a time- and dose-dependent manner. However, the maximal uptake level was much lower than that of ethylnitrosourea (ENU)-induced transformed rat schwannoma-like cells and fibroblasts. By autoradiography of the cultured system, we were able to visualize the accumulation of [3H]2-DG grains in the schwannoma-like cells and fibroblasts, but not in Schwann cells.
Fourth Department of Internal Medicine, Aichi Medical University, Japan.
T
T
Yasuda
G
G
Sobue
T
T
Mitsuma
A
A
Takahashi
D000818:Animals; D002478:Cells, Cultured; D003837:Deoxy Sugars / Q000493:pharmacokinetics*; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D004734:Energy Metabolism; D005038:Ethylnitrosourea / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D003837:Deoxy Sugars; D003847:Deoxyglucose; D005038:Ethylnitrosourea