Plasmacytoma induction in radiochimeras.
We have induced plasmacytomas (MPC) in BALB/c radiochimeras (RCh) repopulated with syngeneic hemopoietic cells carrying distinctive chromosomal markers. A group of 38 RCh that received 0.5 ml pristane, followed by Abelson virus infection 2-3 weeks later, developed 7 tumors (18.5%) of donor origin after a relatively short latency period (X = 83 +/- 8.3 days). In contrast, only 3 (2%) MPCs were observed in 149 RChs that received 0.5 ml pristane 3 times at monthly intervals. Two of them originated from host cells. Pristane-treated RChs developed a less extensive oil granuloma (OG), compared with pristane-treated intact mice. This may explain the low incidence of MPC in the former. Our findings also suggest that Abelson virus can overcome the postulated deficiency of OG. MPC induction in the pristane + Abelson-virus-treated RCh system will facilitate the further characterization of the MPC precursor cell and the localization of genetic resistance vs. susceptibility factors at the donor vs. host level.
Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
S
S
Silva
M
M
Babonits
F
F
Wiener
G
G
Klein
D000818:Animals; D002869:Chromosome Aberrations; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000209:etiology* / Q000235:genetics; D011828:Radiation Chimera