Sperm motile efficiency (SME) of fresh spermatozoa in a hypotonic medium correlates with their cryosurvival in fertile men and infertility patients (predicting test).
The sperm motile efficiency (SME) of fresh spermatozoa in a hypotonic medium was investigated concerning the prediction of post thaw motility and the differentiation between fertile men and infertility patients. A total of 95 semen samples were collected for this study. Twenty of these samples were obtained from semen donors of proven fertility and 75 samples were collected from male partners of infertile couples. There were no significant differences between the SME after mixture of the fresh semen with a 140 mOsm saline in equivalent volumes and after cryopreservation (Wilcoxon-matched pairs signed ranks-test) but a significant positive correlation between the SME after both treatments of semen samples was notable. Significant differences between the fertile control group and group of infertility patients could be determined concerning the SME after mixing of fresh semen with 140 mOsm saline as well as after cryopreservation (Mann-Whitney-U-test). The discriminant analysis revealed a most discriminating effectiveness for SME immediately after the addition of hypoosmotic saline.
Dept. of Dermatology, Karl-Marx-University, Leipzig/GDR.
H J
HJ
Glander
H
H
Bock
P
P
Winiecki
D005615:Freezing; D006801:Humans; D007038:Hypotonic Solutions; D007248:Infertility, Male / Q000473:pathology*; D008297:Male; D009994:Osmolar Concentration; D011237:Predictive Value of Tests; D012965:Sodium Chloride / Q000494:pharmacology; D013081:Sperm Motility; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology*; D014021:Tissue Preservation / Q000379:methods*
D007038:Hypotonic Solutions; D012965:Sodium Chloride