Conditions of radioiodination with iodogen as oxidizing agent.
Different factors that give the maximum iodination of proteins by iodogen method have been evaluated. The quantity of iodogen was varied from 2 to 20 micrograms; pH from 3 to 9; the quantity of bovine serum albumin (BSA) from 25 micrograms to 2 mg; the 131I activity from 200 microCi to 5 mCi and the reaction time from 2 to 30 min. The maximum iodination of proteins is achieved with iodogen-to-iodide molar ratio of 100-200 and iodogen-to-protein molar ratio of 1-2 at pH 6-8 for 10-15 min at room temperature.
Department of Nuclear Medicine, Cleveland Clinic Foundation, OH 44195-5074.
G B
GB
Saha
J
J
Whitten
R T
RT
Go
D000818:Animals; D002417:Cattle; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D012710:Serum Albumin, Bovine; D012711:Serum Albumin, Radio-Iodinated; D014508:Urea / Q000031:analogs & derivatives*
D007202:Indicators and Reagents; D012711:Serum Albumin, Radio-Iodinated; D012710:Serum Albumin, Bovine; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D014508:Urea