Development and application of radioimmunoassays for interleukin-1 alpha and interleukin-1 beta.
Specific and sensitive radioimmunoassay (RIA) systems for human IL-1 alpha and IL-1 beta, using sheep polyclonal antisera, have been developed. The assays, which do not require prior extraction of IL-1, have been used to quantify IL-1 alpha and IL-1 beta in synovial fluid and plasma and to quantify intracellular and extracellular IL-1 alpha and IL-1 beta produced by human monocytes stimulated with endotoxin.
Department of Endocrinology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts, U.K.
S
S
Poole
A F
AF
Bristow
S
S
Selkirk
B
B
Rafferty
D000818:Animals; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000493:pharmacokinetics; D009000:Monocytes / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011994:Recombinant Proteins / Q000032:analysis; D013582:Synovial Fluid / Q000032:analysis
D007375:Interleukin-1; D011994:Recombinant Proteins