Immunohistochemical distribution of beta-protein kinase C in rat hippocampus determined with an antibody against a synthetic peptide sequence.
An antibody directed against a synthetic peptide sequence specific for the beta-subtype of protein kinase C (PKC) was used to determine the distribution of beta-PKC in rat hippocampus by immunocytochemistry. PKC was distributed primarily in the stratum oriens and radiatum of the CA1 region. Positive staining cell bodies were only observed after colchicine treatment in pyramidal cells (CA2-CA4) and granule cells of the dentate gyrus. The discrete localization of various subtypes of PKC should provide clues to their functions.
Naval Medical Research Institute, Bethesda, MD 20814.
B L
BL
Roth
M J
MJ
Iadarola
J P
JP
Mehegan
D M
DM
Jacobowitz
D000595:Amino Acid Sequence; D000818:Animals; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology*; D007106:Immune Sera / Q000276:immunology*; D007150:Immunohistochemistry; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011493:Protein Kinase C / Q000138:chemical synthesis / Q000276:immunology*; D051381:Rats
D007106:Immune Sera; D010446:Peptide Fragments; D011493:Protein Kinase C