A growth factor-responsive gene of murine BALB/c 3T3 cells encodes a protein homologous to human tissue factor.
Polypeptide growth factors rapidly induce the transcription of a set of genes that appear to mediate cell growth. We report that one of the genes induced in BALB/c mouse 3T3 cells encodes a transmembrane protein (mTF) homologous to human tissue factor, which is involved in the proteolytic activation of blood clotting. mTF mRNA is present in many murine tissues and cell lines. Our results raise the possibility that mTF may also play a role in cell growth.
Howard Hughes Medical Institute Laboratory, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
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Hartzell
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Ryder
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Lanahan
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Lau
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Nathan
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000235:genetics; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D005816:Genetic Complementation Test; D006031:Glycosylation; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013925:Thromboplastin / Q000235:genetics*
D000941:Antigens; D006133:Growth Substances; D008565:Membrane Proteins; D012333:RNA, Messenger; D004247:DNA; D013925:Thromboplastin