Accuracy and precision in the determination of Stokes radii and molecular masses of proteins by gel filtration chromatography.
The accuracy and precision of the estimates of hydrodynamic parameters of globular proteins obtained by inverse regression from gel filtration chromatographic data are discussed. The usefulness of gel filtration chromatography as the basis for a rapid and reliable method for the determination of the Stokes radius and the molecular mass is considered. The discussion is supported by an analysis of the models already proposed in the literature, and is based on the precision of the estimates.
Cabré
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Department of Biochemistry and Physiology, University of Barcelona, Catalonia, Spain.
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Canela
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D000818:Animals; D002850:Chromatography, Gel; D004798:Enzymes / Q000032:analysis*; D008433:Mathematics; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*
D004798:Enzymes; D011506:Proteins