Fibronectin on excimer laser and diamond knife incisions.
The experiment was designed to illustrate the difference between the diamond knife and the excimer laser (ArF 193 nm) on keratotomy incisions. The histopathologic effect of fibronectin applied to these wounds was observed. Fibronectin demonstrated a chemotactic effect on fibroblasts and increased the amount of newly synthesized collagen deposited at the incision sites. The excimer laser incisions treated with fibronectin had 43% more fibroblasts per 2,500 microns 2 than the excimer laser incisions not treated with fibronectin. The adjustable diamond knife incisions treated with fibronectin had 42% more fibroblasts than the adjustable diamond knife incisions not treated with fibronectin. The excimer laser ArF 193 nm incisions had 18% more fibroblasts in the incision area than the adjustable diamond knife incisions independent of fibronectin usage. This indicates that the excimer laser incisions and the diamond knife incisions may have different healing mechanisms--perhaps because of the distinctive modes of action of these instruments.
Keates
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RH
Corneal Service, Ohio State University, Columbus 43210.
Bloom
R T
RT
Ren
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Q
Ringel
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DM
Erdey
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RA
D000818:Animals; D002452:Cell Count; D003315:Cornea / Q000187:drug effects / Q000473:pathology; D005347:Fibroblasts / Q000166:cytology; D005353:Fibronectins / Q000494:pharmacology*; D007646:Keratotomy, Radial; D053685:Laser Therapy; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects*
D005353:Fibronectins