[Several properties of human red cells subjected to hypotonic dialysis and resealing to use them as vehicles for bioreactors].
The morphological and metabolic properties of red blood cells submitted to the procedure of loading by hypotonic hemolysis and isotonic resealing were compared with the controls. No appreciable differences could be detected concerning glycolytic ability, the amount of glucose metabolized in the hexose monophosphate pathway and the concentrations of glycolytic intermediates of ATP and of 2,3-DPG. Instead the concentration of reduced glutathione and the MCV were slightly reduced. These manipulated erythrocytes can be used as potential bioreactors or as carriers of exogenous substances.
L
L
Rossi
M
M
Bianchi
G
G
Serafini
M
M
Magnani
D004337:Drug Carriers; D004912:Erythrocytes / Q000378:metabolism* / Q000502:physiology; D006019:Glycolysis; D006461:Hemolysis; D006801:Humans; D008846:Micromanipulation / Q000379:methods
D004337:Drug Carriers
Alcune proprieta' di globuli rossi umani sottoposti a dialisi ipotonica e risigillamento da utilizzare come veicoli o bioreattori.