Specificity of asbestos-induced chromosomal aberrations in short-term cultured human mesothelial cells.
Short-term cultured normal human mesothelial cells were exposed for 48 hours to three different asbestos compounds, crocidiolite, chrysotile, and amosite. In the concentration used (0.01 mg/ml) all three asbestiform minerals caused, within a few days, a significant increase of cells showing numerical and/or structural abnormalities. The abnormalities were analyzed in detail using banding techniques. The results were compared with the cytogenetic observations in 52 published cases of mesotheliomas. This comparison revealed only a few similarities as regards numerical deviations. The structural rearrangements in asbestos-exposed cultures, however, in many instances involved chromosome types and chromosome regions preferentially affected in mesotheliomas.
Department of Pathology and Cytogenetics, Central Hospital, Skövde, Sweden.
K
K
Olofsson
J
J
Mark
D001194:Asbestos / Q000009:adverse effects*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D006801:Humans; D007621:Karyotyping; D008654:Mesothelioma / Q000209:etiology* / Q000235:genetics; D010997:Pleural Neoplasms / Q000209:etiology* / Q000235:genetics
D001194:Asbestos