Serotonin-stimulated protein phosphorylation in aortic smooth muscle cells.
The effects of serotonin on the formation of inositol phosphates and protein phosphorylation were examined in cultured smooth muscle cells. Serotonin stimulated the formation of [3H]inositol monophosphate, [3H]inositol bisphosphate and [3H]inositol trisphosphate. This effect was prevented by 5-HT2 specific antagonist, 6-methyl-1-(1-methylethyl)ergoline-8-carboxylic acid, 2-hydroxy-1-methylpropyl ester [Z]-2-butenedioate (LY53857). Serotonin stimulated the phosphorylation of many polypeptides, among which a 20 kDa polypeptide was the most prominent. The phosphorylation was also inhibited by LY53857. LY53857 alone produced no effects on protein phosphorylation. The 20 kDa polypeptides were also phosphorylated by the addition of 12-O-tetradecanoylphorbol-13-acetate. These results suggest that serotonin stimulates protein phosphorylation through 5-HT2 receptors and possibly activates protein kinase C in intact vascular smooth muscle cells.
Department of Pharmacology, Keio University School of Medicine, Tokyo, Japan.
T
T
Nakaki
B C
BC
Wise
D M
DM
Chuang
R
R
Kato
D000818:Animals; D001011:Aorta; D002478:Cells, Cultured; D004873:Ergolines / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004873:Ergolines; D010750:Phosphoproteins; D012702:Serotonin Antagonists; D012701:Serotonin; D011493:Protein Kinase C; C032663:LY 53857; D013755:Tetradecanoylphorbol Acetate