A method of human diaphorase locus 3 phenotyping in human semen by isoelectric focusing.
A rapid method for the separation of diaphorase (DIA 3) isozymes in the pH range 6-8 is presented. Isoelectric focusing (IEF) of DIA 3 on 0.2 mm thick polyacrylamide gels took 60 min at 5 degrees C. Separated bands are sharp and common phenotypes are readily distinguishable. The method combines high resolution, speed, reproducibility and reagent economy with simplicity.
Pawlowski
R
R
Institute of Forensic Medicine, Medical Academy Gdańsk, Poland.
D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis / Q000235:genetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D008297:Male; D010641:Phenotype; D012661:Semen / Q000201:enzymology*
D007527:Isoenzymes; D008058:Dihydrolipoamide Dehydrogenase