A microcomputer-based system for mapping microvascular networks.
A new method has been developed for mapping microvascular networks using an IBM-AT microcomputer and a video-microscope. After recording a vascular network on video tape, it can be reconstructed off-line using frame by frame analysis. A software tool, developed for mapping and editing networks, is used to prepare a montage of the network using a video cursor and mouse. Vessel lengths and diameters can also be measured from the video image using the same methodology. When the analysis is completed, the vessel network, together with a tabulation of lengths and diameters of individual vessels, can be displayed on a TV monitor or reduced to hard copy. This system provides a simple method for quantitative studies of arteriolar, venular and capillary networks.
Wu
C H
CH
University of Arizona, Department of Physiology, Arizona Health Sciences Center, Tucson 85724.
Johnson
P C
PC
D000818:Animals; D003196:Computer Graphics; D007091:Image Processing, Computer-Assisted; D008833:Microcirculation / Q000033:anatomy & histology*; D008838:Microcomputers; D009132:Muscles / Q000098:blood supply; D014743:Videotape Recording