[Studies on cell surface fibronectin of transformed Syrian hamster lung cells induced by butyric acid].
In this paper, Fn was analysed qualitatively and quantitatively in three Syrian hamster cell lines, ie, nontransformed baby hamster lung fibroblasts cell line (BHL), a transformed cell line (BHLB4) and a butyric acid-induced phenotypically reversed cell line (ButB4) respectively. Fn was visualized on cell surface by means of indirect immunofluorescence technique. Immunofluorescence of Fn on the surface of BHL was bright with a stripe-like distribution, while that on the surface of BHLB4 was very dim or dispersed. On ButB4 cell surface, the intensity and distribution of immunofluorescence was similar to that on BHLB4 cells. Fn was isolated by affinity chromatography from the cell surface of the three cell lines. Its molecular weight was 250 kDa on SDS-polyacrylamide electrophoresis. The quantity of Fn isolated from surface of ButB4 was a bit lower than that from BHL, but was much higher than that from BHLB4. The result offers us a useful criterion for transformation and reverse transformation.
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D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002846:Chromatography, Affinity; D006224:Cricetinae; D005347:Fibroblasts / Q000032:analysis; D005353:Fibronectins / Q000032:analysis*; D008168:Lung / Q000032:analysis* / Q000166:cytology; D008647:Mesocricetus
D002087:Butyrates; D005353:Fibronectins; D020148:Butyric Acid