A protein that binds to a cis-acting element of wheat histone genes has a leucine zipper motif.
The structure and function of transcription factors of higher plants was studied by isolating cDNA clones encoding a wheat sequence-specific DNA binding protein. A hexameric nucleotide motif, ACGTCA, is located upstream from the TATA box of several plant histone genes. It has been suggested that this motif is essential for efficient transcription of the wheat histone H3 gene. A wheat nuclear protein, HBP-1 (histone DNA binding protein-1), which specifically binds to the hexameric motif, has previously been identified as a putative transcription factor. A cDNA clone encoding HBP-1 has been isolated on the basis of specific binding of HBP-1 to the hexameric motif. The deduced amino acid sequence indicates that HBP-1 contains the leucine zipper motif, which represents a characteristic property of several eukaryotic transcription factors.
Tabata
T
T
Department of Botany, Faculty of Science, Kyoto University, Japan.
Takase
H
H
Takayama
S
S
Mikami
K
K
Nakatsuka
A
A
Kawata
T
T
Nakayama
T
T
Iwabuchi
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005796:Genes; D005809:Genes, Regulator; D006657:Histones / Q000235:genetics*; D007256:Information Systems; D007930:Leucine; D008745:Methylation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics
D004268:DNA-Binding Proteins; D006657:Histones; D009687:Nuclear Proteins; C062189:histone DNA binding protein-1; D004247:DNA; D007930:Leucine