Alternative splicing of the platelet-derived growth factor A-chain transcript occurs in normal as well as tumor cells and is conserved among mammalian species.
Using a polymerase chain reaction approach, we have analyzed the alternative usage of the platelet-derived growth factor A-chain exon 6 in mRNA from various cell types. The results show that this sequence is utilized in a small fraction of the mRNA molecules in normal as well as transformed cells and that this phenomenon is conserved among mammalian species.
Department of Pathology, University Hospital, Uppsala, Sweden.
B
B
Matoskova
F
F
Rorsman
V
V
Svensson
C
C
Betsholtz
D000818:Animals; D005075:Biological Evolution; D015139:Blotting, Southern; D004259:DNA-Directed DNA Polymerase; D005091:Exons; D005347:Fibroblasts / Q000378:metabolism; D005784:Gene Amplification; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D008322:Mammals / Q000235:genetics; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D004259:DNA-Directed DNA Polymerase