A rapid method for detecting Trichinella spiralis larvae in pork using a monoclonal antibody-latex conjugate.
A simple, non-microscopic method was developed for the direct detection of Trichinella spiralis in pork. Samples of meat were treated with pepsin for 2 h to liberate the larvae, and sonicates were made from the deposit and used in an antigen detection system. In this system, latex particles, sensitized with a parasite-specific monoclonal antibody, were utilized in a slide or tube agglutination test. The efficiencies of these immunological methods were compared with that of trichinelloscopy in the examination of 102 pork specimens, 35 of which contained varying numbers of larvae after enzymatic digestion of 2 g of the meat. Trichinelloscopy detected 77.1% of these positive samples, but this was lower (55.5 and 41.7%) for those samples with a low larval burden (less than 50 or less than 10 larvae g-1 tissue, respectively). Better results (88.6, 76.5 and 60.0%) were obtained with the slide latex agglutination technique. The efficiency of detection was further improved (100%) using the more sensitive tube agglutination method. However, this was based on a small number (20) of samples examined and the limit of detection of this technique was subsequently found to be three larvae in total. No false-positives were seen with the latex agglutination methods from a total of 67 uninfected specimens examined.
Department of Microbiology, University of Hong Kong, Queen Mary Hospital Compound.
W F
WF
Choy
P L
PL
Lim
M H
MH
Ng
D000818:Animals; D000911:Antibodies, Monoclonal; D003964:Diaphragm; D007841:Latex Fixation Tests; D008460:Meat; D009132:Muscles / Q000469:parasitology*; D011237:Predictive Value of Tests; D013552:Swine; D014234:Trichinella / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal