Improved assay for quantitating adherence of ruminal bacteria to cellulose.
A quantitative technique suitable for the determination of adherence of ruminal bacteria to cellulose was developed. This technique employs adherence of cells to cellulose disks and alleviates the problem of nonspecific cell entrapment within cellulose particles. By using this technique, it was demonstrated that the adherence of Ruminococcus flavefaciens FD1 to cellulose was inhibited by formaldehyde, methylcellulose, and carboxymethyl cellulose. Adherence was unaffected by acid hydrolysates of methylcellulose, glucose, and cellobiose.
Department of Animal Sciences, University of Illinois, Urbana 61801.
M A
MA
Rasmussen
B A
BA
White
R B
RB
Hespell
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001422:Bacterial Adhesion; D001439:Bacteroides / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism; D005051:Eubacterium / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000378:metabolism; D010459:Peptococcaceae / Q000378:metabolism; D012417:Rumen / Q000382:microbiology*
D002482:Cellulose