A miniaturised method for immunoglobulin allotype serology.
A modification of the classic haemagglutination inhibition method for immunoglobulin allotyping is described which employs reagent volumes of 2 microliters compared with the 25 microliter volumes commonly used in other serological allotyping methods. No loss of sensitivity or specificity was associated with the miniaturised method when compared to the standard tile technique. This method simplifies the large scale phenotyping and screening of sera and permits considerable savings of valuable reagents to be made.
Regional Tissue Typing Laboratory, Welsh Regional Transfusion Centre, Cardiff, Wales, U.K.
N T
NT
Young
J
J
Street
C
C
Darke
D005787:Gene Frequency; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000032:analysis* / Q000235:genetics; D008832:Microchemistry; D014852:Wales
D007126:Immunoglobulin Allotypes