Alkaline elution of DNA from stomach pyloric mucosa of rats treated with glyoxal.
DNA damage in the pyloric mucosa of the stomach of male F344 rats was determined by the alkaline elution method after administration of glyoxal, a direct-acting mutagen present in various heated foods, by gastric intubation. Glyoxal at doses of 50-550 mg/kg body weight induced DNA damage in the pyloric mucosa of rat stomach, detected by a 5- to 12-fold increase in the elution rate constant 2 h after its administration. N-Methyl-N'-nitro-N-nitrosoguanidine, a glandular stomach carcinogen, used as a positive control at doses of 1-100 mg/kg body weight induced a 11- to 24-fold increase in the elution rate constant, while 2-acetylaminofluorene, which is not a gastric carcinogen, given as a negative control at doses of 200-400 mg/kg body weight did not increase the elution rate constant. Thus glyoxal, which was previously suggested to induce unscheduled DNA synthesis in the pyloric mucosa of rat stomach, was confirmed to be genotoxic in this region.
Department of Molecular Oncology, University of Tokyo, Japan.
C
C
Furihata
A
A
Hatta
Y
Y
Sato
T
T
Matsushima
D000447:Aldehydes / Q000633:toxicity*; D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D004249:DNA Damage; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006037:Glyoxal / Q000633:toxicity*; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens; D051381:Rats; D011916:Rats, Inbred F344
D000447:Aldehydes; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D006037:Glyoxal; D004247:DNA