Isolation and complete primary sequence of a new ovine wild-type beta-lactoglobulin C.
A new wild type of beta-lactoglobulin has been identified in the milk of sheep. It has been designated as ovine beta-lactoglobulin C. Its primary structure has been determined by direct protein microsequencing of intact protein and RP-HPLC-derived tryptic peptides. The new beta-lactoglobulin C is a subtype of ovine beta-lactoglobulin A with a single exchange Arg-Gln at position 148. This exchange may influence polymerisation of beta-lactoglobulin since in the crystal structure of orthorhombic bovine beta-lactoglobulin, residues 145-150 constitute a short beta-sheet region involved in dimer formation by pairing of dyad-related strands.
Erhardt
G
G
Justus-Liebig-Universitat, Institut für Tierzucht und Haustiergenetik, Giessen.
Godovac-Zimmermann
J
J
Conti
A
A
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007525:Isoelectric Focusing; D007782:Lactoglobulins / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D008892:Milk / Q000032:analysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D012756:Sheep; D014357:Trypsin
D000596:Amino Acids; D007782:Lactoglobulins; D010446:Peptide Fragments; D014357:Trypsin