Determination of multiple forms of cytochrome P-450 in microsomes from the digestive gland of Cryptochiton stelleri.
Polyclonal antibodies raised against purified trout cytochromes P-450 (P-450) LM2 (anti-LM2) and LM4b (anti-LM4b) were used in Western blot analyses with digestive gland microsomes from control and beta-naphthoflavone (BNF)-treated gumboot chitons Cryptochiton stelleri. An increase and decrease in staining intensity subsequent to treatment with anti-LM4b and anti-LM2, respectively, was observed in digestive gland microsomes from BNF-treated chiton. Thus, there appears to be at least two forms of P-450 in microsomes from the digestive gland of Cryptochiton; one of which is induced by BNF and perhaps is involved in benzo(a)pyrene (BP) biotransformation, and another form which is inhibited by BNF.
Schlenk
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Toxicology Program, Oregon State University, Corvallis 97331.
Buhler
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D000818:Animals; D001571:Benzoflavones; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004790:Enzyme Induction / Q000187:drug effects; D008861:Microsomes / Q000201:enzymology*; D008974:Mollusca / Q000201:enzymology*; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System