Technique for cellular fluorescence distribution analysis.
The usefulness of multidimensional slit-scan flow cytometry in whole cell measurements is dependent on extracting relevant features from the cellular fluorescence distributions (slit-scan contours). In addition, the extraction of these features must be rapid to allow for real-time data processing during acquisition. This paper describes two algorithms that have been used successfully to count the numbers of local maxima (peaks) and to find nuclear boundaries in a cellular fluorescence distribution. These routines are efficient, use only simple integer arithmetic, and have been implemented on several different microprocessors.
Department of Pathology, University of Rochester Medical Center, New York 14642.
R D
RD
Robinson
J E
JE
Reeder
L L
LL
Wheeless
D000465:Algorithms; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D006801:Humans