The optimal processing of plasma samples for the determination of bicyclo-PGE in patients with malignant maxillofacial tumors.
Prostaglandins (PGs) have been shown to be increased in several tumor tissues. PGE2 and PGF1-alpha Radioimmunoassays (RIAs) have been widely used for quantitative PG-measurements in cancer patients. Our results indicate that optimal processing of plasma samples for the determination of bicyclo-PGE2 is of the highest importance in order to get reproducible results. Therefore it is necessary to fulfill the following requirements: 1. 30 minutes rest before sampling; 2. Avoid venous occlusion; 3. Use constant needle diameter; 4. Precooled anticoagulant; 5. Addition of a cyclooxygenase inhibitor is necessary; 6. Control of processing temperature; 7. Storage at -70 degrees C less than -20 degrees C; 8. Control of storage time; 9. The samples have to be examined after thawing once; 10. Only blood samples processed under the above discovered optimal conditions are of clinical relevance; 11. An international standardization for the processing of plasma samples seems to be necessary considering all these requirements; 12. In 41 patients with maxillo-facial carcinomas the PG-value was significantly higher than in the controls; 13. A RIA-evaluation methodologically properly done may be used as an additional aid for clinical monitoring of the patients.
Department of Dentistry, University of Vienna, Austria.
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A
Nell
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H
Porteder
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M
Matejka
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H
Sinzinger
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000925:Anticoagulants / Q000494:pharmacology; D001800:Blood Specimen Collection; D015232:Dinoprostone / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006258:Head and Neck Neoplasms / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D005082:Physical Exertion; D011863:Radioimmunoassay; D013048:Specimen Handling; D013696:Temperature
D000925:Anticoagulants; C025088:11-deoxy-15-keto-13,14-dihydro-11 beta,16-cycloprostaglandin E2; D015232:Dinoprostone