The effect of crosslinking by bis(3,5-dibromosalicyl) fumarate on the autoxidation of hemoglobin.
Bis(3,5-dibromosalicyl) fumarate was used to crosslink hemoglobin both in the oxy and deoxy states. This double headed diaspirin was known to crosslink oxy Hb A selectively between Lys 82 beta 1 and Lys 82 beta 2 (Walder, J. A., et al. (1979) Biochemistry 18, 4265) and deoxy Hb A between Lys 99 alpha 1 and Lys 99 alpha 2 (Chatterjee R. Y., et al. (1986) J. Biol. Chem. 261, 9929). The autoxidation at 37 degrees C of oxy alpha 99 crosslinked hemoglobin was found to be 1.8 times as fast as that of Hb A while that of the oxy beta 82 crosslinked hemoglobin was only 1.2 times as fast. After 5 hours the formation of methemoglobin in the alpha crosslinked Hb A is 21.3% compared to 10.8% in beta crosslinked Hb A and 6.4% in Hb A. These results may effect the proposed use of alpha 99 crosslinked hemoglobin as a blood substitute by demonstrating the need for protection from autoxidation during storage.
Department of Chemistry Loyola University of Chicago, IL 60626.
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D001241:Aspirin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D006441:Hemoglobin A / Q000378:metabolism*; D007700:Kinetics; D008706:Methemoglobin / Q000378:metabolism; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism
D003432:Cross-Linking Reagents; D010108:Oxyhemoglobins; C021630:bis(3,5-dibromosalicyl)fumarate; D008706:Methemoglobin; D006441:Hemoglobin A; D001241:Aspirin