Influence of supplementation of saturated alkanes on the membrane properties of Microsporum gypseum.
Microsporum gypseum cells grown on saturated alkanes of different chain lengths (C10, C12, C14, C16 and C18) exhibited increased levels of total phospholipids and sterols. A significant increase in the content of phosphatidylcholine was observed in alkane-grown cells. Increased saturation of phospholipid fatty acid was observed with all the alkanes studied, which was mainly due to the decreased amount of C18:1 and C18:2 with concomitant increase in the levels of palmitic acid. The affinity for glycine changed in alkane-supplemented cells as compared to glucose-grown cells. 1-Anilino-naphthalene-8-sulphonate (ANS) binding to the spheroplast membrane demonstrated increased binding sites in supplemented cells. These results are discussed in terms of the effect of altered lipid composition on the membrane structure and function of this fungus.
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Vaidya
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Khuller
D000473:Alkanes / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D008563:Membrane Lipids / Q000378:metabolism*; D008864:Microsporum / Q000187:drug effects*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism; D013329:Structure-Activity Relationship
D000473:Alkanes; D008563:Membrane Lipids; D010743:Phospholipids