Characterization of two anti-DNA antibodies bearing distinct idiotypes. Correlation with clinical manifestations.
Spontaneous idiotype shift of anti-DNA antibodies in systemic lupus erythematosus was shown to be associated with changes in the clinical manifestations of the disease. Characterization of two anti-DNA antibody preparations from the same lupus patient showed that lupus nephritis was associated with antibodies showing higher specific antigen-binding activity and avidity to DNA and presenting more cationic quality upon isoelectric focusing and Western blot analysis than the ones found in patients without renal involvement. Lupus cerebritis was associated with anti-DNA antibodies with different idiotypic characteristics not shared by anti-DNA antibodies present in lupus nephritis. Lupus cerebritis anti-DNA antibodies had less specific antigen-binding activity and avidity to DNA and did not show cationic quality. Idiotypic and immunochemical changes of anti-DNA antibodies in lupus may be associated with various clinical manifestations.
Department of Medicine, University of Kansas Medical Center, Kansas City 66103.
R
R
Suenaga
P A
PA
Munoz
S H
SH
Hon
N I
NI
Abdou
D000915:Antibody Affinity; D001323:Autoantibodies; D004247:DNA / Q000276:immunology*; D004277:DNA, Single-Stranded / Q000276:immunology; D004660:Encephalitis / Q000150:complications / Q000276:immunology; D005260:Female; D006801:Humans; D007130:Immunoglobulin Idiotypes; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000276:immunology*; D008181:Lupus Nephritis / Q000276:immunology
D001323:Autoantibodies; D004277:DNA, Single-Stranded; D007130:Immunoglobulin Idiotypes; D004247:DNA