A rapid response to a plant hormone: auxin stimulates phospholipase A2 in vivo and in vitro.
Addition of the active auxins indole-3-acetic acid, 2,4-dichlorophenoxyacetic acid or alpha-naphthylacetic acid to cultured soybean (Glycine max L.) cells prelabeled with ethanolamine or choline increased the radioactivity in the lysophosphatidylethanolamine (LPE) or lysophosphatidylcholine (LPC) pool within 5 min. The inactive auxin analogue, beta-naphthylacetic acid, was inactive in this response. In membranes prelabeled in vivo, either with ethanolamine or choline, and subsequently isolated from zucchini (Cucurbita pepo L.) hypocotyls, indole-3-acetic acid and 2,4-dichlorophenoxyacetic acid stimulated the conversion of phosphatidylethanolamine (PE) to LPE and of phosphatidylcholine (PC) to LPC in vitro whereas the inactive auxin analogue 2,3-dichlorophenoxyacetic acid did not.
Scherer
G F
GF
Botanisches Institut, Universität Bonn, FRG.
André
B
B
D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D007210:Indoleacetic Acids / Q000494:pharmacology*; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008246:Lysophospholipids / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010937:Plant Growth Regulators / Q000494:pharmacology*; D013025:Glycine max; D013329:Structure-Activity Relationship
D007210:Indoleacetic Acids; D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D010937:Plant Growth Regulators; C008301:lysophosphatidylethanolamine; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2