Flufenamic acid senses conformation and asymmetry of human erythrocyte band 3 anion transport protein.
With Cl as substrate, the human red blood cell anion transport (band 3) protein can exist in four conformations: Ei, with the transport site facing the cytoplasm; Eo, with the transport site facing the external medium; and ECli and EClo, the corresponding forms loaded with Cl. Flufenamic acid (FA), an inhibitor that binds to an external site different from the transport site, binds to Eo with a dissociation constant of 0.0826 +/- 0.0049 (SE) microM. Binding of iodide or sulfate to the external-facing transport site reduces the affinity by 1.66 or 14.3-fold, respectively. Changing from Eo to Ei lowers the affinity by 3.7-fold, and binding of cytoplasmic iodide to Ei further decreases the affinity by 5.5-fold. Thus changes in orientation of the transport site and substrate binding, even at the opposite side of the membrane, cause sufficient conformational changes in band 3 to affect FA binding substantially. If the possible effects of Cl binding to the transport site on FA affinity are estimated from the iodide data, the dependence of FA inhibitory potency on Cl concentrations inside and outside the cell suggests that there are at least 6.5 times as many inward-facing as outward-facing Cl-loaded transport sites. This information can be used to calculate the distribution of capnophorin among the various conformations under different circumstances and to devise conditions for recruiting the transport molecules toward a particular conformation.
Department of Biophysics, University of Rochester School of Medicine and Dentistry, New York 14642.
P A
PA
Knauf
L J
LJ
Spinelli
N A
NA
Mann
D000328:Adult; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism / Q000648:ultrastructure*; D027321:Anion Transport Proteins; D002352:Carrier Proteins / Q000378:metabolism / Q000648:ultrastructure*; D002712:Chlorides / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism; D005439:Flufenamic Acid / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007454:Iodides / Q000378:metabolism; D007700:Kinetics; D008433:Mathematics; D008962:Models, Theoretical; D011485:Protein Binding; D011487:Protein Conformation
D001457:Anion Exchange Protein 1, Erythrocyte; D027321:Anion Transport Proteins; D002352:Carrier Proteins; D002712:Chlorides; D007454:Iodides; D005439:Flufenamic Acid