Effects of fibrinogen degradation fragments D and E on cell-mediated immunity.
Serum taken from severely traumatized victims suppresses in vitro the response of normal lymphocytes to the mitogenic stimulant phytohemagglutinin (PHA). In the postburn period, fibrin degradation products (fragments D and E) are elevated in a high percentage. Controversy exists as to whether these fragments contribute to what is clinically evident as cell-mediated immune (CMI) suppression. Purified fragments D and E were isolated over an ion exchange cellulose column after activating, with streptokinase, a solution containing fibrinogen and plasminogen. Lymphocytes from six volunteers were cultured with PHA and serial dilutions of fragments D and E; each was analyzed for ability to incorporate radiolabeled thymidine. Fragment E possessed in vitro CMI suppression at pharmacologic doses. Fragment D demonstrated immune suppressive capabilities at doses approximating those estimated to occur in the acute postburn phases of injury.
Ferrara
J J
JJ
Department of Surgery, University of South Alabama, Mobile 36617.
Kan
C
C
Luterman
A
A
Curreri
P W
PW
D005338:Fibrin Fibrinogen Degradation Products / Q000494:pharmacology*; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D007121:Immunocompetence / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D010835:Phytohemagglutinins
D005338:Fibrin Fibrinogen Degradation Products; D010835:Phytohemagglutinins; C015214:fibrinogen D fragment; C018543:fibrinogen fragment E