Human serum immuno-reactive copper, zinc-superoxide dismutase assayed with an enzyme monoclonal immunosorbent in patients with digestive cancer.
Two types of monoclonal antibodies, N4 and N6, were raised against human copper, zinc-superoxide dismutase (Cu,Zn-SOD). An enzyme-linked immunosorbent assay for Cu,Zn-SOD was developed in sandwich method using these monoclonal antibodies. The measurable assay range was 1.0-100 micrograms/l and the coefficient of variation within and between series were 7.5% and 8.1%. Sera of 154 healthy control subjects displayed a low level (14 micrograms/l) of immunoreactive Cu,Zn-SOD with a logarithmic normal distribution. On the other hand, serum Cu,Zn-SOD levels were significantly elevated in patients with various digestive cancers.
Oka
S
S
First Department of Internal Medicine, Yamaguchi University School of Medicine, Ube, Japan.
Ogino
K
K
Matsuura
S
S
Yoshimura
S
S
Yamamoto
K
K
Okazaki
Y
Y
Takemoto
T
T
Kato
N
N
Uda
T
T
D000911:Antibodies, Monoclonal; D004067:Digestive System Neoplasms / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D013482:Superoxide Dismutase / Q000097:blood*
D000911:Antibodies, Monoclonal; D013482:Superoxide Dismutase