Acrylamide binding to the DNA and protamine of spermiogenic stages in the mouse and its relationship to genetic damage.
Mice received an intraperitoneal injection of 14C-labeled acrylamide (AA) at an exposure of 125 mg/kg to equal that used in genetic studies carried out by Shelby et al. (1986). Subsequently, spermatozoa were recovered from the reproductive tracts of the animals over a 3-week period and assayed for the amount of bound AA. A strong increase in the level of binding occurred in late-spermatid to early-spermatozoa stages; these same stages are also genetically most sensitive to the action of AA. At all time points, alkylation of DNA within the sperm accounted for a very small fraction (generally less than 0.5%) of the total sperm-head alkylation. However, alkylation of protamine, a protein unique to sperm cells, was found to be correlated with total sperm-head alkylation and accounted for essentially all of the AA binding. Two radioactive adducts were found in hydrolysed protamine samples, one of which co-eluted with a standard of S-carboxyethylcysteine. Protamine alkylation appears to be a significant cause of acrylamide-induced genetic damage in spermiogenic cells of the mouse.
Biology Division, Oak Ridge National Laboratory, TN 37831-8077.
G A
GA
Sega
R P
RP
Alcota
C P
CP
Tancongco
P A
PA
Brimer
D020106:Acrylamide; D000178:Acrylamides / Q000378:metabolism* / Q000633:toxicity; D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage; D004822:Epididymis / Q000166:cytology; D006868:Hydrolysis; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009153:Mutagens / Q000378:metabolism*; D011479:Protamines / Q000235:genetics / Q000378:metabolism*; D013077:Sperm Head / Q000187:drug effects / Q000378:metabolism; D013091:Spermatogenesis / Q000187:drug effects*; D014649:Vas Deferens / Q000166:cytology
D000178:Acrylamides; D000596:Amino Acids; D009153:Mutagens; D011479:Protamines; D020106:Acrylamide; D004247:DNA