Evidence for a change in expression of DNA ligase genes in the Pleurodeles waltlii germ line during gonadogenesis.
The expression of DNA ligase genes was studied using the nuclear transplantation approach in the germ line of Pleurodeles waltlii (P. waltlii) just before and during gonadogenesis. Germ cell (GC) nuclei were isolated from larvae of P. waltlii and transplanted into unfertilized Ambystoma mexicanum eggs. DNA ligase activity in these eggs was then analyzed after sucrose gradient fractionation. The activity of DNA ligase I (heavy form, 7.5 S) of P. waltlii was present when the transplanted GC nuclei were isolated before the first histological appearance of gonadogenesis. At the beginning of genital ridge formation and thereafter, DNA ligase I activity was replaced by that of DNA ligase II (light form, 7 S). Expression of form I was found to be sensitive to inhibitors of translation and transcription, while that of form II was not. Therefore, the change in DNA ligase activity of the transferred nuclei of P. waltlii germ cells was assumed to be the consequence of a change in gene activity, namely, the repression of the gene encoding DNA ligase I. This change in the gene-regulated state could be linked to protein modifications of the chromatin. These results indicate that, at the beginning of gonadogenesis, germ cells receive information leading to a new state of differentiation.
Laboratoire de Biologie animale, Université Pierre et Marie Curie, Paris, France.
M
M
Lesimple
J C
JC
David
C
C
Dournon
J
J
Lefresne
C
C
Houillon
J
J
Signoret
D000557:Ambystoma / Q000254:growth & development; D000818:Animals; D011088:DNA Ligases / Q000096:biosynthesis* / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D005854:Germ Cells / Q000637:transplantation; D006066:Gonads / Q000201:enzymology* / Q000254:growth & development; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D010999:Pleurodeles / Q000254:growth & development* / Q000378:metabolism; D011118:Polynucleotide Ligases / Q000096:biosynthesis*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012455:Salamandridae / Q000254:growth & development*; D013096:Spermine / Q000494:pharmacology
D007527:Isoenzymes; D011500:Protein Synthesis Inhibitors; D013096:Spermine; D011088:DNA Ligases; D011118:Polynucleotide Ligases