Evaluation of platelet concentrates prepared from buffy coats and stored in a glucose-free crystalloid medium.
Comparison was made between platelet concentrates prepared from pools of buffy coats removed from standard blood donations and stored in a glucose-free, commercially available crystalloid solution (BC-PCs) and standard platelet concentrates prepared from platelet-rich plasma (PRP-PCs). Platelet yield in BC-PCs and PRP-PCs was 59 and 75 percent of donated platelets, respectively. The number of total white cells in 1 BC-PC unit, prepared from a pool of 7 buffy coats, was 21 x 10(6), i.e., 50 times lower than that of 7 units of PRP-PCs. The in vitro values of adequate platelet quality were maintained for 10 days in BC-PCs stored in 1000-mL polyolefin bags. Prolonged bleeding times were reduced or corrected in three of three thrombocytopenic leukemic patients evaluated before and after transfusion of stored BC-PCs. Pretransfusion and 1- and 24-hour posttransfusion median platelet counts in 57 leukemic recipients during 4 months of routine transfusion of BC-PCs (n = 93) were 14, 35, and 27 x 10(9) per L, while those of PRP-PCs (n = 246) were 13, 37, and 31 x 10(9) per L, respectively. No reactions to BC-PCs were reported, but a 1.3 percent rate of reaction to PRP-PC transfusions was reported. This study indicates that BC-PCs are a good alternative to PRP-PCs for platelet support of thrombocytopenic patients.
Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Policlinico, Milan, Italy.
F
F
Bertolini
P
P
Rebulla
D
D
Riccardi
M
M
Cortellaro
M L
ML
Ranzi
G
G
Sirchia
D000328:Adult; D001793:Blood Preservation / Q000379:methods; D001803:Blood Transfusion / Q000379:methods; D002469:Cell Separation / Q000379:methods; D000077324:Crystalloid Solutions; D005947:Glucose; D006801:Humans; D007552:Isotonic Solutions; D007938:Leukemia / Q000628:therapy; D007958:Leukocyte Count; D007962:Leukocytes; D010865:Pilot Projects; D010952:Plasma Substitutes; D010976:Platelet Count; D017713:Platelet Transfusion; D012996:Solutions
D000077324:Crystalloid Solutions; D007552:Isotonic Solutions; D010952:Plasma Substitutes; D012996:Solutions; D005947:Glucose