A rapid and sensitive micro-assay to determine the capacity of quinones to undergo redox cycling.
Using a series of aziridinyl-benzoquinones it is shown that the conversion of oxyhemoglobin to methemoglobin in sheep erythrocytes is correlated with the capacity of each quinone to undergo redox cycling. Based on these findings a semiquantitative assay is developed for the rapid screening of redox cycling quinones.
Department of Pharmacognosy, Faculty of Pharmacy, University of Utrecht, The Netherlands.
L A
LA
Hart
N A
NA
van der Wal
A S
AS
Koster
R P
RP
Labadie
D000818:Animals; D001388:Aziridines; D016227:Benzoquinones; D002849:Chromatography, Gas; D053138:Cyclohexenes; D004912:Erythrocytes / Q000378:metabolism; D008706:Methemoglobin / Q000096:biosynthesis; D008832:Microchemistry; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism; D011809:Quinones / Q000097:blood / Q000378:metabolism*; D012756:Sheep
D001388:Aziridines; D016227:Benzoquinones; D053138:Cyclohexenes; D010108:Oxyhemoglobins; D011809:Quinones; C006052:2,5-bis(1-aziridinyl)-3,6-bis(2-methoxyethoxy)-4-benzoquinone; D008706:Methemoglobin