An effective method for plasma lipoprotein separation: studies of various animal species.
1. Plasma lipoprotein separation by density gradient ultracentrifugation largely depends on visual examination based on the natural yellow pigments of lipoproteins. 2. In non-human species and in humans with dyslipoproteinemia, some lipoproteins are not well visualized due to the lack of pigments. 3. Using a fluorescent probe in minute quantity (1,6 diphenyl-1,3,5-hexatriene) we were able to demonstrate an effective plasma lipoprotein separation using a discontinuous density gradient ultracentrifugation technique. 4. Plasma lipoproteins of human, chicken, rat and carp were compared showing the unique character of carp HDL.
Aviram
M
M
Lipid Research Unit, Rambam Medical Center, Technion Haifa, Israel.
Mokady
S
S
Cogan
U
U
D000818:Animals; D002347:Carps; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002784:Cholesterol / Q000097:blood; D004161:Diphenylhexatriene; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D051381:Rats; D013045:Species Specificity; D014280:Triglycerides / Q000097:blood
D005456:Fluorescent Dyes; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D004161:Diphenylhexatriene; D002784:Cholesterol