Identification of the trans-stilbene oxide-active glutathione transferase in human mononuclear leukocytes and in liver as GST1.
A glutathione transferase from human mononuclear leukocytes with a high activity toward trans-stilbene oxide (GT-tSBO) has been studied in liver and blood from fetus and adults and in blood from neonates. Using starch gel electrophoresis, different phenotypes of GST1 have been determined, GST1 0, GST1 1, and GST1 2. As judged from activity measurements and the fact that only those individuals who express the null allele of GST1, the GST1 0, which has a low activity toward trans-stilbene oxide, it is concluded that the hepatic transferase GST1 is identical to GT-tSBO, as well as to hepatic transferase mu. In addition, it has been shown that the different genotypes of GST1 1 (GST1 1-1, GST1 1-0) and GST1 2 (GST1 2-2, GST1 2-0) can be separated by measuring the GT-tSBO activity in whole blood from the same individual. It is also demonstrated that GT-tSBO activity is much lower in fetal liver, approximately 10 times, compared with adult liver, while this activity seems to be unchanged in the blood from fetus and adults, as well as in neonates.
Wallenberg Laboratory, Molecular Ecogenetics, Lund, Sweden.
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Seidegård
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RW
Pero
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Stille
D000328:Adult; D004137:Dinitrochlorobenzene; D004592:Electrophoresis, Starch Gel; D005333:Fetus / Q000201:enzymology; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D010641:Phenotype; D013267:Stilbenes
D004137:Dinitrochlorobenzene; D013267:Stilbenes; D005982:Glutathione Transferase; C025906:stilbene oxide