Hemoglobin radiolabeling: in vitro and in vivo comparison of iodine labeling with iodogen and a new method for technetium labeling.
Radiolabeled hemoglobin may be a useful tool in the study of the body distribution of hemoglobin solutions developed as plasma expanders with oxygen-transporting capacity. The present investigation compares the suitability of two radiolabeling techniques for hemoglobin. 125I labeling of hemoglobin with Iodogen as iodinating agent caused major changes in the chromatographic behaviour and an accelerated plasma clearance of the labeled hemoglobin in rats. A recently developed two-step procedure for 99mTc labeling gave better results. The label had only minimal influence on the chromatographic behaviour of hemoglobin. In vivo, no free label occurred in the circulation and no transfer of the label to other plasma proteins took place. The plasma clearance of 99mTc-labeled hemoglobin in rats was slowed. However, this could be explained entirely by diminishing glomerular filtration, probably by inhibition of the dissociation of the hemoglobin molecule into dimers. The plasma clearance of hemoglobin modified by intramolecular cross-linking, which prevents dissociation of the molecule into dimers and thus excretion by the kidney, was not influenced by the label. We conclude that the 99mTc labeling procedure is suitable for in vivo distribution studies of hemoglobin when it is taken into account that the urinary excretion is underestimated. For cross-linked hemoglobin, which is more promising as plasma expander, no such restriction exists.
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
W K
WK
Bleeker
J
J
Van der Plas
R I
RI
Feitsma
J
J
Agterberg
G
G
Rigter
E K
EK
Pauwels
J C
JC
Bakker
D000818:Animals; D005260:Female; D006454:Hemoglobins; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D010952:Plasma Substitutes / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains; D013667:Technetium; D014018:Tissue Distribution; D014508:Urea / Q000031:analogs & derivatives*
D006454:Hemoglobins; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D010952:Plasma Substitutes; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D013667:Technetium; D014508:Urea