Use of DMSO for the cryopreservation of Tritrichomonas foetus in liquid nitrogen.
Four Tritrichomonas foetus strains were successfully preserved in liquid nitrogen at -196 degrees C in the presence of 10% dimethylsulfoxide (DMSO) for 7 months (Strain 1) and 6 months (Strain 2, 3 and 4). The percentage of viable organisms after thawing ranged between 65 and 85%, depending upon the strain. The method used is described in detail.
Departamento de Producción Animal EEA INTA Balcarce C.C., Buenos Aires, Argentina.
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D000818:Animals; D002417:Cattle; D004121:Dimethyl Sulfoxide; D005615:Freezing; D009584:Nitrogen; D011309:Preservation, Biological; D014318:Tritrichomonas / Q000502:physiology*
D009584:Nitrogen; D004121:Dimethyl Sulfoxide