[Mutagenicity testing in the sperm head test/mouse and mutagenic potency of 2 disinfectants on the basis of peracetic acid and phenols, respectively].
For the improvement of the registration of data by assessment of mutagenic risk the analysis of sperm-head abnormalities have been rendered as a suitable at indirect evidence of mutagenic potency. The reproduction of the results is well. The sensitivity of the test is high. The quantitative evaluation of genotoxic effects is possible. By reason of comparatively low methodical expense the screening investigations of species mouse are suitable, before more expensive tests will be accomplished for the elucidation of the mutation type. At 35 day-long intraperitoneal application the double number of sperm head abnormalities was approximately induced through Wofasteril (dose refer to main agent peracetic acid 2.6 mg/kg bw/d) and about 1/3 through cyclophosphamide (dose 25 mg/kg bw/d). With a 50% reduction of Wofasteril dose the mutagenic threshold-concentration was too low. The phenolic disinfectant Wofasept caused only slight no significant increase of the rate of sperm-head abnormalities (doses refer to the content of compounds, for Chlorocresol 1.6 mg/kg bw/d, for Clorofen 0.7 mg/kg bw/d and for detergent 4.8 mg/kg bw/d).
Koch
S
S
Institut für Allgemeine und Kommunale Hygiene des Bereiches Medizin Ernst-Moritz-Arndt-Universität Greifswald.
Kramer
A
A
Stein
J
J
Adrian
V
V
Weuffen
W
W
D000085:Acetates / Q000633:toxicity*; D000818:Animals; D003520:Cyclophosphamide / Q000633:toxicity; D004202:Disinfectants / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009152:Mutagenicity Tests; D010463:Peracetic Acid / Q000633:toxicity*; D010636:Phenols / Q000633:toxicity*; D013077:Sperm Head / Q000187:drug effects*; D013094:Spermatozoa / Q000002:abnormalities* / Q000187:drug effects*
D000085:Acetates; D004202:Disinfectants; D010636:Phenols; D003520:Cyclophosphamide; D010463:Peracetic Acid
Mutagenitätsprüfung im Spermakopf-Test/Maus und mutagene Potenz von 2 Desinfektionsmitteln auf Basis von Peressigsäure bzw. Phenolen.