[Phosphorylation of components of high molecular weight aminoacyl-tRNA-synthetase complex from the rabbit liver by associated casein kinase type I].
The aminoacyl-tRNA synthetase complex from rabbit liver possesses an endogenous protein kinase activity. The associated protein kinase in the complex was defined as casein kinase I. Using FPLC, a fraction of the supramolecular complex with a high level of metabolic activity was isolated; this preparation was found to be enriched in the casein kinase activity. Incubation of this fraction with [gamma-32P] ATP leads to the intensive incorporation of labeled phosphate into 12 polypeptides of the complex, i.e., glutamyl-, isoleucyl-, leucyl-, methionyl-, lysyl-, arginyl- and aspartyl-tRNA synthetases. An addition of free homologous casein kinase I does not change the spectrum or level of phosphorylation of the complex substrates. The homologous casein kinase II phosphorylates polypeptides with Mr of 65, 43 and 20 kDa in the complex.
S M
SM
Elizarov
A D
AD
Vol'fson
Iu A
IuA
Motorin
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D047388:Casein Kinases; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits
D010455:Peptides; D011494:Protein Kinases; D047388:Casein Kinases; D000604:Amino Acyl-tRNA Synthetases
Fosforilirovanie komponentov vysokomolekuliarnogo aminoatsil-tRNK-sintetaznogo kompleksa iz pecheni krolika assotsiirovannoĭ kazeinovoĭ kinazoĭ tipa I.