Studies of spontaneous proliferation of mononuclear cells in human immunodeficiency virus (HIV-1)-infected individuals.
We evaluated the ability of mononuclear cell populations from 56 hemophiliac subjects, 69 homosexuals, and 32 control subjects to proliferate spontaneously in vitro. Levels of spontaneous proliferation (SP) were not significantly different among homosexuals or hemophiliac subjects when they were grouped as asymptomatic human immunodeficiency virus (HIV) seronegative, asymptomatic seropositive, persistent generalized lymphadenopathy, acquired immunodeficiency syndrome-related complex, or acquired immunodeficiency syndrome groups. There was, however, a stepwise increase in levels of SP corresponding with progression of disease in both patient groups. No correlations were noted between SP and CD8+ cell populations or mitogenic responses in study subjects, but SP correlated inversely to percentages and numbers of CD4+ lymphocytes. Depletion of CD4+ cells from mononuclear cell populations of HIV-infected subjects decreased SP. Thus, in HIV-1-infected subjects, SP increases as HIV-1-related disease progresses and appears to be CD4 dependent.
Clinical Immunology Section, Tulane University School of Medicine, New Orleans, La.
R D
RD
deShazo
J E
JE
Morgan
C B
CB
Daul
A
A
Andes
N
N
Hyslop
C
C
Palmer
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1; D006467:Hemophilia A / Q000276:immunology; D006716:Homosexuality; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins