Comparative effect on humoral response of four different proteins covalently linked on BSA-containing liposomes.
The humoral response to encapsulated BSA appears to be a classical TD antigen response with a high ratio of IgG to IgM, whereas that to covalently-linked antigen is more complex, characterized by an enhanced synthesis of IgM, leading to an equal production of IgM and IgG. In a recent paper, we observed that surface-linked Con A on BSA-containing liposomes changed the isotype distribution to encapsulated BSA so as to mimic the response to surface-linked antigen. In the present study, we compared the immune response to BSA in BALB/c mice immunized with the antigen encapsulated into liposomes coated with one of four different proteins: Con A, Myo, MSA, or PWM. The humoral response was analyzed by measurements of antibody production (total Ig, IgM, and IgG isotypes) on serum samples obtained by cardiac puncture. It can be concluded from our results that any surface-linked protein may affect the interaction between liposome-associated antigen and immunocompetent cells.
Shahum
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Département Chimie-Biologie, Université du Québec à Trois-Rivières, Canada.
Thérien
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D000818:Animals; D000917:Antibody Formation; D003208:Concanavalin A / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008081:Liposomes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009211:Myoglobin / Q000276:immunology; D010743:Phospholipids / Q000032:analysis; D011043:Pokeweed Mitogens / Q000276:immunology; D011506:Proteins / Q000276:immunology*; D012709:Serum Albumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008081:Liposomes; D009211:Myoglobin; D010743:Phospholipids; D011043:Pokeweed Mitogens; D011506:Proteins; D012709:Serum Albumin; D003208:Concanavalin A; D012710:Serum Albumin, Bovine