[Analysis of genomic similarity in Streptomycetes belonging to the fluorescent subgroup as a confirmation of their taxonomic revision using a population method].
Genomic similarity was analysed in streptomycetes belonging to the fluorescent subgroup: Streptomyces chrysomallus, S. fluorescens, S. galbofluorescens and S. citreofluorescens. The degree of reference S. chrysomalius DNA hybridization with S. fluorescens and S. galbofluorescens DNAs was 75 and 82%, respectively, thus being within the limits of the intraspecial hybridization level. S. citreofluorescens DNA showed a 55% homology with reference S. chrysomallus DNA, which corresponded to the range of interspecies hybridization. These conclusions were confirmed by the results obtained in analysing the thermostability of hybrid duplexes. Therefore, these findings are consistent with the data of revising the species taxonomy of this streptomycetes subgroup which was done using the method of comparative population analysis. The population model proposed by one of the authors can be used to assess the intraspecies level of DNA-DNA hybridization.
S N
SN
Filippova
V D
VD
Kuznetsov
T P
TP
Turova
T L
TL
Ivanova
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005453:Fluorescence; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000145:classification / Q000235:genetics*; D013696:Temperature
D004269:DNA, Bacterial
Analiz skhodstva genomov streptomitsetov fluorestsiruiushcheiĭ podgruppy kak podtverzhdennie ikh taksonomicheskoĭ revizii populiatsionnym metodom.