Characterization of retinyl beta-glucuronide in human blood.
Retinyl beta-glucuronide has been identified as a normal endogenous component of human blood, as have retinoyl beta-glucuronide and retinoic acid. The mean serum concentration of retinyl beta-glucuronide in adult volunteers (n = 6) was 6.8 +/- 4.0 nmol/L (means +/- SD) with a range from 1 to 11 nmol/L. Purified serum retinyl beta-glucuronide was characterized by the mass spectrum of its methylated trimethylsilyl derivative, by its hydrolysis by beta-glucuronidase, and by its chromatographic behavior in high-performance liquid chromatography systems. During a single day, concentrations of retinyl beta-glucuronide, retinoyl beta-glucuronide, and retinoic acid varied approximately two-fold.
Department of Biochemistry, Iowa State University, Ames 50011.
A B
AB
Barua
R O
RO
Batres
J A
JA
Olson
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D012176:Retinoids / Q000097:blood / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000097:blood
D012176:Retinoids; C026787:retinoyl glucuronide; D014212:Tretinoin