Mitogenic effect of estradiol on MCF-7 human breast cancer cells can be modulated by serum.
The MCF-7 human breast cancer cell line responds to estradiol stimulation in vitro by increased proliferation only if prolonged subcultures in dextran-coated charcoal-treated fetal calf serum have been made previously. This growth stimulation is not obtained when cells are grown in medium containing 5% untreated fetal calf serum. We describe here the culture conditions under which we obtain a reproducible estradiol effect on cell growth.
Laboratoire de Biochimie, Faculté de Pharmacie, Université de Limoges, France.
A
A
Najid
A
A
Nicolas
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M
Tixier
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G
Habrioux
D001943:Breast Neoplasms / Q000473:pathology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003470:Culture Media / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008934:Mitogens / Q000494:pharmacology*; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D003470:Culture Media; D008934:Mitogens; D013629:Tamoxifen; D004958:Estradiol