On the use of fibroblasts in tissue culture to study the neurochemistry of brain mechanisms controlling blood pressure.
Fibroblasts grown in tissue culture from skin biopsies taken from the genetic model of spontaneously hypertensive rats (SHR), show a reduction in the accumulation of choline when compared to fibroblasts grown from skin biopsies taken from normotensive Wistar Kyoto rats. Choline uptake by fibroblasts from both strains of rats is sodium dependent but there is no difference between the strains in the effects of sodium depletion. These results suggest that fibroblasts in tissue culture may be a useful source of tissue for analyzing differences between hypertensive and normotensive individuals that are genetically determined rather than due to prolonged exposure to elevated blood pressure. In addition, the fibroblast shares several biochemical characteristics with the neuron and, at least as far as these common characteristics are concerned, appears to be a good model for neurochemical study.
Richardson
J S
JS
Department of Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Dunlop
D M
DM
D000818:Animals; D002478:Cells, Cultured; D002794:Choline / Q000493:pharmacokinetics*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006973:Hypertension / Q000378:metabolism*; D009638:Norepinephrine / Q000493:pharmacokinetics*; D051381:Rats; D011918:Rats, Inbred SHR / Q000378:metabolism*; D011919:Rats, Inbred Strains / Q000378:metabolism*; D012867:Skin / Q000166:cytology; D012964:Sodium / Q000502:physiology
D012964:Sodium; D002794:Choline; D009638:Norepinephrine