Improved neonatal heart preservation with an intracellular cardioplegia and storage solution.
The optimal conditions for preservation of the neonatal heart for transplantation remain uncertain. An isolated, working neonatal piglet heart model was used to compare a standard extracellular-like cardioplegic solution followed by storage at 4 degrees C in normal saline for 12 hr (n = 8) to cardioplegia and storage at 4 degrees C for 24 hr in an intracellular-like solution (n = 7). Seven of eight hearts in the 12-hr Extracellular Group failed to regain function, with a maximum stroke work index (SWI), developed at a left ventricular end-diastolic pressure (LVEDP) of 9 mm Hg of 0.91 +/- 0.30 x 10(3) erg/g (mean +/- standard error of the mean), 7.1% of nonpreserved control hearts. In contrast, all hearts arrested and stored for 24 hr in the intracellular solution regained function with a maximum SWI, again at a LVEDP of 9 mm Hg of 9.51 +/- 1.98 X 10(3) erg/g, 73.7% of control (P less than 0.05). Ultrastructural changes seen by electron microscopy paralleled the functional results. We conclude that an intracellular arrest and storage solution may be superior to conventional solutions for extended preservation of the neonatal heart.
Breda
M A
MA
Department of Surgery, University of California Medical Center, Los Angeles 90024-1741.
Drinkwater
D C
DC
Laks
H
H
Bhuta
S
S
Wu
A
A
Pearl
J
J
Chang
P
P
D000818:Animals; D000831:Animals, Newborn; D001769:Blood; D002314:Cardioplegic Solutions / Q000493:pharmacokinetics / Q000494:pharmacology*; D003326:Coronary Circulation; D006321:Heart / Q000502:physiology; D006324:Heart Arrest, Induced; D006334:Heart Function Tests; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure; D011309:Preservation, Biological; D013552:Swine
D002314:Cardioplegic Solutions