Plasma lipoprotein metabolism in lean and in fat chickens produced by divergent selection for plasma very low density lipoprotein concentration.
Plasma lipoprotein metabolism was studied in vivo in two lines of chickens produced by selection for high and low plasma very low density lipoprotein (VLDL) concentration. Rates of VLDL secretion were measured by determining the rate of accumulation of triglyceride in the plasma after intravenous injection of anti-lipoprotein lipase antibody. The clearance of VLDL-triglyceride and its uptake into liver and adipose tissue was examined using radioactively labeled VLDL synthesized in vivo. The rate of VLDL secretion was about threefold higher in the high-VLDL line as compared to the leaner, low VLDL-line (6.7 vs 2.1 mumol VLDL triglyceride/h per ml of plasma). The clearance of VLDL from the circulation of the low VLDL line was much faster than that of the high VLDL line (t1/2 of 3.7 and 13.6 min, respectively). The proportion of administered radiolabel taken up by the abdominal fat pad was substantially greater in the fat line than in the lean line (11.9 vs 4.8%, respectively). Lipoprotein lipase activities in leg muscle and heart were consistently greater in the low-VLDL line and beta-hydroxybutyrate concentrations in the plasma of the low-VLDL line were significantly greater than those in the high-VLDL line (0.86 vs 0.48 mumol/ml). The results show that the approximately tenfold difference in plasma VLDL concentration between lines is primarily due to markedly different rates of hepatic VLDL production and that selection has made a major effect on partitioning of VLDL triglyceride between adipose and other tissues.(ABSTRACT TRUNCATED AT 250 WORDS)
Agricultural and Food Research Council's Institute for Grassland and Animal Production, Poultry Department, Roslin, Midlothian, Scotland.
H
H
Griffin
F
F
Acamovic
K
K
Guo
J
J
Peddie
D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D004032:Diet; D008071:Lipoprotein Lipase / Q000276:immunology / Q000378:metabolism; D008074:Lipoproteins / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008657:Metabolic Clearance Rate; D009765:Obesity / Q000201:enzymology / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D008071:Lipoprotein Lipase