Chloroplast ribosomal DNA organization in the chromophytic alga Olisthodiscus luteus.
There are almost no data describing chloroplast genome organization in chromophytic (chlorophyll a/c) plants. In this study chloroplast ribosomal operon placement and gene organization has been determined for the golden-brown alga Olisthodiscus luteus. Ribosomal RNA genes are located on the chloroplast DNA inverted repeat structure. Nucleotide sequence analysis, demonstrated that in contrast to the larger spacer regions in land plants, the 16S-23S rDNA spacer of O. luteus is only 265 bp in length. This spacer contains tRNA(Ile) and tRNA(Ala) genes which lack introns and are separated by only 3 bp. The sequences of the tRNA genes and 16S and 23S rDNA termini flanking the spacer were examined to determine homology between O. luteus, chlorophytic plant chloroplast DNA, and prokaryotes.
Delaney
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TP
University of Washington, Department of Botany, Seattle 98195.
Cattolico
R A
RA
D001345:Autoradiography; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D002736:Chloroplasts; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D056890:Eukaryota / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D015183:Restriction Mapping
D015342:DNA Probes; D004275:DNA, Ribosomal; D015347:RNA Probes; D004247:DNA