Low polarity ligands of sex hormone-binding globulin in pregnancy. Part II--Identification.
Certain previously unrecognized ligands of SHBG of low polarity in pregnancy were identified. They include two weakly bound compounds: 5 alpha-pregnane-3,20-dione and progesterone; and two strongly bound substances, 2-methoxyestrone and a new steroid, estradienolone (17 beta-hydroxy-1,5-estradiene-3-one). The identification of the first three peaks was based on chromatographic elution patterns, binding characteristics and gas chromatography-mass spectrometry. The identification of the fourth peak, the new steroid, was based on similar kinds of evidence and, in addition, solubility characteristics and ultraviolet absorption spectrum.
Reproductive Physiology Unit, Montreal General Hospital, Canada.
A
A
Philip
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BE
Murphy
D043582:5-alpha-Dihydroprogesterone; D002851:Chromatography, High Pressure Liquid; D004963:Estrenes / Q000032:analysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis; D006894:Hydroxyestrones / Q000032:analysis; D007745:Labor Onset; D009277:Nandrolone / Q000032:analysis; D011247:Pregnancy / Q000097:blood*; D011277:Pregnanediones / Q000032:analysis; D011374:Progesterone / Q000032:analysis; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*
D004963:Estrenes; D006894:Hydroxyestrones; D011277:Pregnanediones; D012738:Sex Hormone-Binding Globulin; D011374:Progesterone; D009277:Nandrolone; D043582:5-alpha-Dihydroprogesterone; C003655:2-methoxyestrone; D006854:Hydrocortisone