Radiosensitivity of human prostate cancer and malignant melanoma cell lines.
The relative radioresponsiveness of human prostate cancer compared to malignant melanoma is well known. The effects of beta-estradiol or testosterone on the X-irradiation survival of several human cell lines were studied, including: human prostate carcinoma cell lines PC3 and DU145 and human malignant melanoma cell lines A375 and A875. Lines PC3 and DU145 demonstrated 55-61 fmol per 10(6) cells of androgen receptor with no detectable estrogen or progesterone receptor. Cells were irradiated at 120 cGy/min dose rate. There was no detectable toxicity of up to 10(-4) M testosterone or beta-estradiol on PC3 or DU145 cells in the absence of X-irradiation. At plating efficiencies from 11-13%, and plating densities of 1 x 10(4) cells per 60 cm2 flask, cell lines PC3 and DU145 demonstrated a Do of 108.5 +/- 6.5, n 2.1 +/- 0.7 cGy, and Do of 143.5 +/- 1.5 cGy, n 2.4 +/- 0.5, respectively. The addition of testosterone or beta-estradiol at 10(-4) to 10(-10) M prior to or after, X-irradiation did not alter radiosensitivity. At the same dose rate of 120 cGy/min, malignant melanoma cell lines A375 and A875 had a Do of 125 +/- 2.5 cGy, n 1.56 +/- 0.8 SF2 0.65 +/- 0.03 and line A875 demonstrated a Do of 129 +/- 4.5 cGy, n 1.58 +/- 0.4 SF2 0.55 +/- 0.04, respectively. The radiosensitivity of melanoma cell lines did not decrease at low dose rate 5 cGy/min. Thus, the in vitro radiosensitivity of androgen receptor positive prostate cancer cell lines is not necessarily altered by the presence of androgen before or after irradiation. The data support the concept that all malignant melanoma cell lines do not show a broad-shouldered cell survival curve in vitro and intrinsic cellular radioresistance.
Wollin
M
M
Department of Urology, University of Massachusetts Medical Center, Worcester 01655.
FitzGerald
T J
TJ
Santucci
M A
MA
Menon
M
M
Longcope
C
C
Reale
F
F
Carlson
J
J
Sakakeeny
M A
MA
Greenberger
J S
JS
D002470:Cell Survival / Q000528:radiation effects; D004958:Estradiol / Q000494:pharmacology; D006801:Humans; D008297:Male; D008545:Melanoma / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D011836:Radiation Tolerance; D013739:Testosterone / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D013739:Testosterone; D004958:Estradiol