Improved separation speed and efficiency for proteins, nucleic acids and viruses in asymmetrical flow field flow fractionation.
The performance of the asymmetrical flow field-flow fractionation channel has been improved by the use of a much thinner (0.12-mm) channel than before and by flow programming (stepwise gradient elution). The thinner channel contributes to a decreased zone broadening which enables complete resolution in a shorter time. Three protein peaks, representing molecular weights from 12,000 to 136,000, wer completely resolved within 3 min. Flow programming speeds up the elution of the high-molecular-weight materials which occur late in a fractogram. This enabled separation of a small plasmid fragment (700 base pairs) from the large amounts of a big fragment (4600 base pairs) in 30 min and improved detection of a presumed trimer of albumin. Two viruses (1.8 10(6) and 50 10(6) daltons) were eluted as narrow peaks within 5 min.
Department of Analytical Pharmaceutical Chemistry, University of Uppsala Biomedical Center Sweden.
A
A
Litzén
K G
KG
Wahlund
D005591:Chemical Fractionation / Q000379:methods; D009696:Nucleic Acids / Q000032:analysis*; D010957:Plasmids; D011506:Proteins / Q000032:analysis*; D014780:Viruses / Q000302:isolation & purification*
D009696:Nucleic Acids; D011506:Proteins