[Lectin binding pattern of the normal rat lens].
Paraffin sections of normal rat lenses were stained with seven lectins, using an avidin-biotin-complex (ABC) procedure to study the presence and distribution of specific glycoconjugate in the lens. Epithelial cells were stained with concanavalin A (Con A) and wheat germ agglutinin (WGA). Lens fibers with nuclei were stained with six lectins: peanut agglutinin (PNA), ricinus communis agglutinin (RCA), soybean agglutinin (SBA), dolichos biflorus agglutinin (DBA), ConA and WGA. The equatorial cortex stained heavily, while lens fibers without nuclei stained only slightly. Ulex europeus agglutinin (UEA-I) caused no staining at all. Con A stained the nuclear membrane of lens fibers. These results suggest that glycoconjugate changes during lens cell differentiation, and that high levels of N-acetyl-glucosamine and mannose are distributed in both lens epithelium and lens fibers, but that N-acetyl-galactosamine distribution level is low in the lens epithelium.
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Suzuki
D000818:Animals; D002454:Cell Differentiation; D006001:Glycoconjugates / Q000378:metabolism; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism*; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D006001:Glycoconjugates; D037102:Lectins