Microcapillary clonogenic assays for human marrow hematopoietic progenitor cells.
The capillary clonogenic cell assay was developed and adapted to culture myeloid and erythroid colonies from human bone marrow cells. The plating efficiencies for femoral bone marrow granulocyte-macrophage progenitors (CFU-gm), erythroid colony-forming units (CFU-e) and erythroid burst-forming units (BFU-e) were 0.143%, 0.229% and 0.141%, respectively. Standard bone marrow progenitor Petri dish assays require a total culture volume of 1 ml per dish, and as such are not suitable for the small numbers of cells often obtained from human bone marrow samples. The microcapillary assay as developed and standardized in our laboratory has the unique advantage of being able to utilize small numbers of cells. This technique is suitable for evaluating the myelotoxicity of investigational new anti-cancer and anti-HIV agents and for further investigation of the mechanisms underlying chemotherapy-induced bone marrow toxicity.
Hipple Cancer Research Center, Dayton, Ohio 45439-2092.
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Volpe
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Murphy
D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans