Ontogeny and implication of pregnancy-associated agglutinin of the rat uterus.
The mannose/fructose-binding agglutinin from Day 1-7 post coitum (p.c.) rat uteri was purified on Concanavalin A. The specific haemagglutination activity peaked on Days 4 and 5 p.c. and a 1.4-fold increase in the yield was accompanied by a 10-12-fold increase in specific agglutination titre. The mannose-binding affinity of the protein also increased, but the highest fructose-binding affinity was found on Day 1 p.c., which may indicate a role of the protein in fructose concentration for utilization by the spermatozoa. Rats that were pseudopregnant, superovulated and pseudopregnant, and had one uterine horn ligated showed that, although a basal level of the protein was induced by the hormonal milieu, actual stimulation of the protein synthesis occurred in the presence of the fertilized ova.
Mukhopadhyay
P K
PK
Indian Institute of Chemical Biology, Calcutta.
Chowdhury
M
M
D000818:Animals; D002846:Chromatography, Affinity; D010064:Embryo Implantation; D005260:Female; D005632:Fructose / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*; D011270:Pregnancy, Animal / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000032:analysis* / Q000378:metabolism; D015053:Zygote / Q000502:physiology
D011257:Pregnancy Proteins; D005632:Fructose; D008358:Mannose