Catecholamines in CSF, plasma, and tissue after autologous transplantation of adrenal medulla to the brain in patients with Parkinson's disease.
Catecholamine concentrations were measured in tissue samples of caudate and adrenal medulla in eight patients with Parkinson's disease who were taking L-dopa and were undergoing autologous transplantation of adrenal medulla to caudate nucleus. High-performance liquid chromatography with electrochemical detection was used for the measurement of analytes. Dopamine concentrations were quite similar in the caudate and the adrenal medulla; epinephrine and norepinephrine concentrations were some 600 times and 90 times higher, respectively, than that of dopamine in adrenal medulla but were barely detectable in caudate nucleus. Catecholamines and metabolites were also measured, before and after transplantation, in lumbar cerebrospinal fluid (CSF) and plasma 1 hour after the patients' first morning dose of L-dopa. The major fractions of the catecholamines in CSF were sulfoconjugated. The concentrations of sulfoconjugated but not free dopamine were modestly increased in CSF after the transplantation, although plasma concentrations were unchanged. CSF concentrations of free and conjugated norepinephrine and epinephrine, 3-methoxy-4-hydroxyphenylglycol, and homovanillic acid were unchanged after the transplantation. The data suggest that the grafted tissue does not retain its noradrenergic or adrenergic properties after transplantation, and that dopamine formation in the brain may be modestly increased. Plasma catecholamines were unaffected after the removal of one adrenal gland for the transplant.
Tyce
G M
GM
Department of Physiology, Mayo Clinic, Rochester, MN 55905.
Ahlskog
J E
JE
Carmichael
S W
SW
Chritton
S L
SL
Stoddard
S L
SL
van Heerden
J A
JA
Yaksh
T L
TL
Kelly
P J
PJ
D000313:Adrenal Medulla / Q000378:metabolism / Q000637:transplantation*; D000328:Adult; D002395:Catecholamines / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D002421:Caudate Nucleus / Q000378:metabolism; D004298:Dopamine / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism; D004837:Epinephrine / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000134:cerebrospinal fluid; D008875:Middle Aged; D009638:Norepinephrine / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism; D010300:Parkinson Disease / Q000378:metabolism / Q000601:surgery*; D014182:Transplantation, Autologous
D002395:Catecholamines; D008734:Methoxyhydroxyphenylglycol; D004298:Dopamine; D009638:Norepinephrine; D006719:Homovanillic Acid; D004837:Epinephrine