IL-3 and stromal cell-derived factor synergistically stimulate the growth of pre-B cell lines cloned from long-term lymphoid bone marrow cultures.
The addition of IL-3 to modified Whitlock-Witte long-term lymphocyte cultures was found to enhance the growth of a small but significant number of B cell precursors supported by an adherent stromal cell monolayer. Several pre-B cell lines were cloned from IL-3-treated long-term lymphocyte cultures. The growth requirements and physical properties of one representative clone, BL/3, are described. BL/3 cells were shown to be unresponsive to IL-3 except when it is used at very high concentrations. In contrast, significant growth was stimulated by stromal cell conditioned medium previously shown to contain a pre-B cell growth factor. Optimal growth of the pre-B cell clone was stimulated by stromal cell conditioned medium plus IL-3. Synergy between the stromal cell-derived factor and IL-3 occurred when IL-3 was used over a wide range of concentrations including a relatively low amount that was ineffective as a growth stimulus by itself. The finding that more than one factor is required to sustain optimal growth of some pre-B cells parallels the complex growth requirements reported for some primitive myeloid/erythroid progenitors.
Rennick
D
D
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304.
Jackson
J
J
Moulds
C
C
Lee
F
F
Yang
G
G
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification / Q000166:cytology / Q000187:drug effects*; D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells / Q000145:classification / Q000166:cytology / Q000187:drug effects; D046508:Culture Techniques / Q000379:methods; D004357:Drug Synergism; D005109:Extracellular Matrix / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000166:cytology / Q000187:drug effects*; D007377:Interleukin-3 / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell
D007377:Interleukin-3; D011947:Receptors, Antigen, B-Cell