Different Hoechst 33342 and DAPI fluorescence of the human Y chromosome in bivariate flow karyotypes.
The staining properties of AT-specific dyes Hoechst 33342 and DAPI as revealed by Hoechst 33342/mithramycin and mithramycin/DAPI bivariate human flow karyotype patterns are different for chromosomes rich in heterochromatin. The peak corresponding to chromosome Y of a given cell line is higher on the A/T axis with mithramycin/DAPI staining than with mithramycin/Hoechst. The chromosome 1 was found slightly more fluorescent in mithramycin/DAPI than in mithramycin/Hoechst as judged by the slight displacement of its area on the Hoechst-DAPI axis. The other peaks did not show major differences. On the same flow karyotypes, chromosomal rearrangements were detected.
Laboratoire de Cytogénétique, U3OI INSERM and L.O.I., UM7 CNRS, Paris, France.
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A
Bernheim
R
R
Miglierina
D001562:Benzimidazoles; D002461:Cell Line, Transformed; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles; D007621:Karyotyping; D014998:Y Chromosome
D001562:Benzimidazoles; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; C017807:bisbenzimide ethoxide trihydrochloride