Differential expression and subcellular localization for subunits of cAMP-dependent protein kinase during ram spermatogenesis.
The expression of mRNAs for the RI alpha, RII alpha, and C alpha subunits of cAMP-dependent protein kinase has been studied in different ram germ cells. The sizes of the specific RI alpha, RII alpha, and C alpha mRNAs, observed in germ cells were 1.6, 2.0, and 2.6 kb, respectively. RI alpha and C alpha mRNAs were mainly expressed in primary spermatocytes. A postmeiotic expression predominating in early spermatids was unique to RII alpha mRNA. The location of RI, RII alpha, and C subunits in well-defined organelles of ram spermatids and epididymal sperm was assessed by immunogold electron microscopy. In spermatids, RI, RII alpha, and C were essentially present in the forming acrosome and, to a lesser extent, in the nucleus. During sperm epididymal maturation, the protein kinases disappeared from the acrosome and were detected in a variety of sperm functional areas, such as the tip of the acrosome, the motility apparatus, and the membrane network. The present study on subunits of cAMP-dependent protein kinase supports the concept that specific functions are attached to the different subunits in that it shows differential expression and differential subcellular localization in germ cells.
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
C
C
Pariset
J
J
Feinberg
J L
JL
Dacheux
O
O
Oyen
T
T
Jahnsen
S
S
Weinman
D000818:Animals; D015342:DNA Probes; D006651:Histocytochemistry; D006801:Humans; D046911:Macromolecular Substances; D008297:Male; D008854:Microscopy, Electron; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012756:Sheep; D013087:Spermatids / Q000201:enzymology* / Q000648:ultrastructure; D013091:Spermatogenesis; D013094:Spermatozoa / Q000201:enzymology* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000201:enzymology; D013737:Testis / Q000201:enzymology / Q000648:ultrastructure
D015342:DNA Probes; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011494:Protein Kinases