Structural characterization of an anti-complementary pectic polysaccharide from the roots of Bupleurum falcatum L.
An anti-complementary pectic polysaccharide (BR-2-IIb), isolated from the roots of Bupleurum falcatum L., has an average molecular weight of 36,000 (gel filtration), and was subjected to methylation analysis before and after carboxyl-reduction, digestion with endo-polygalacturonase, base-catalysed beta-elimination, and partial acid hydrolysis. BR-2-IIb consisted mainly of galacturonic acid, arabinose, rhamnose, and galactose in the molar ratios 13.0:2.1:1.4:1.0 and contained a large enzyme-sensitive polygalacturonan region. The enzyme-resistant region (PG-1) was rich in neutral sugars and contained a backbone of 4-linked GalA and 2-linked Rha to which a highly branched arabinogalactan was attached to position 4 of some 2-linked Rha units. Partial acid hydrolysis of BR-2-IIb gave Ara-(1----3)-Ara, Ara-(1----4)-Arap, Ara-(1----5)-Araf, Ara-(1----6)-Gal, Gal-(1----4)-Gal, GalA-(1----2)-Rha, GalA-(1----4)-Rha, GalA----Rha----Rha, Gal----Rha----Rha, and GlA-(1----6)-Gal in addition to (1----4)linked oligogalacturonides. The anticomplementary activity of BR-2-IIb was enhanced by de-esterification, but carboxyl-reduction decreased the activity.
Oriental Medicine Research Center of the Kitasato Institute, Tokyo, Japan.
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H
Yamada
K S
KS
Ra
H
H
Kiyohara
J C
JC
Cyong
Y
Y
Otsuka
D002240:Carbohydrate Sequence; D003169:Complement Inactivator Proteins / Q000032:analysis / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010936:Plant Extracts; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D003169:Complement Inactivator Proteins; D010936:Plant Extracts; D011134:Polysaccharides