Wheat phosphoglycerate kinase: evidence for recombination between the genes for the chloroplastic and cytosolic enzymes.
We have isolated and sequenced cDNA clones containing the entire coding region of both the chloroplast and cytosolic versions of phosphoglycerate kinase from wheat. Comparison of these sequences reveals a higher than expected level of similarity between the nucleic acids and encoded proteins. Analysis of this data in relation to that for phosphoglycerate kinase sequences of mammals, prokaryotes and yeasts suggests that the wheat genes have recombined. This has resulted in the chloroplast and cytosolic kinases being more similar to each other than would be expected if the chloroplast enzyme had evolved directly from that of a prokaryotic progenitor.
Longstaff
M
M
Institute of Plant Science Research, Cambridge, UK.
Raines
C A
CA
McMorrow
E M
EM
Bradbeer
J W
JW
Dyer
T A
TA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000201:enzymology*; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D003600:Cytosol / Q000201:enzymology; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005796:Genes; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000201:enzymology / Q000235:genetics
D003062:Codon; D007527:Isoenzymes; D004247:DNA; D010735:Phosphoglycerate Kinase