Development and evaluation of a new polymeric material for small caliber vascular prostheses.
Polyethylene oxide (PEO), because of its low levels of protein and cellular adsorption, may provide a suitable coating for synthetic small caliber vascular prostheses. PEO/polysiloxane networks were synthesized via an acid-catalyzed epoxy/hydroxyl crosslinking reaction and used to produce conduits with a 4-mm internal diameter. Three networks with nominal PEO molecular weights of 2000, 8000, and 20,000 and all 65% PEO by weight were studied. Blood compatibility was assessed by measuring 111In-platelet and 125I-fibrinogen deposition in a baboon ex vivo shunt, over a 1-hr time period and at a flow rate of 50 ml/min. Differences in material performance were noted particularly after the initial 30-min blood contact period. Materials in the mid and high PEO molecular weight range (8000 and 20,000) had significantly lower levels of platelet adsorption than networks of low PEO molecular weight (2000) at 30 min (P less than 0.005) and 60 min (P less than 0.05). The lowest level of platelet deposition was noted on networks of high PEO molecular weight (20,000). During the observation period, platelet accumulation on this surface was less than one platelet per 1000 microns. Platelet deposition on Gore-Tex was two and three orders of magnitude greater than that on the high molecular weight PEO material at 30 and 60 min, respectively (P less than 0.001). Fibrinogen adsorption was also lower on materials of mid and high PEO molecular weights, when compared with low molecular weight networks (P less than 0.05) and Gore-Tex (P less than 0.05). Scanning electron micrographs confirmed these observations. Overall, platelet and fibrinogen depositions are low for PEO networks, particularly for materials of high PEO molecular weight. This latter observation may be related to increased surface molecular mobility and a relative enhancement of PEO content at the blood-material interface.
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge.
E L
EL
Chaikof
J E
JE
Coleman
K
K
Ramberg
R J
RJ
Connolly
E W
EW
Merrill
A D
AD
Callow
D000818:Animals; D001672:Biocompatible Materials; D001769:Blood; D001792:Blood Platelets / Q000502:physiology; D001807:Blood Vessel Prosthesis / Q000592:standards*; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D005340:Fibrinogen / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning; D010215:Papio; D011092:Polyethylene Glycols; D011108:Polymers
D001672:Biocompatible Materials; D011108:Polymers; D011092:Polyethylene Glycols; D005340:Fibrinogen