Comparison of media for screening of diarrheic stools for the recovery of Clostridium difficile.
Recoveries of Clostridium difficile from stool specimens by using three media, cycloserine-mannitol agar (M-CMA), cycloserine-mannitol-blood agar (M-CMBA), and cycloserine-cefoxitin agar (M-CCA), were compared. Of 321 clinical specimens, 37 yielded C. difficile. Thirty-four were positive on M-CCA, 21 were positive on M-CMA, and 20 were positive on M-CMBA. M-CCA recovered significantly more C. difficile than did M-CMBA or M-CMA.
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68105.
P C
PC
Iwen
S J
SJ
Booth
G L
GL
Woods
D003013:Clostridium / Q000254:growth & development / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003470:Culture Media; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology*; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans
D003470:Culture Media