Characterization of melanin-concentrating hormone from rat hypothalamus.
A melanin-concentrating hormone (MCH)-like peptide was isolated from rat hypothalamus by acid extraction, gel filtration chromatography, immunoaffinity chromatography using antiserum directed against salmon MCH, and two steps of HPLC using octadecyl columns. Several zones of immunoreactivity were isolated, and Edman degradation in a gas phase sequencer indicated that the amino acid sequence of all zones was identical. Rat hypothalamic MCH is a nonadecapeptide which differs from salmon MCH by an N-terminal extension of two amino acids and four additional substitutions. Rat MCH has the following primary structure: Asp-Phe-Asp-Met-Leu-Arg-Cys-Met-Leu-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Gln- Val.
Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, San Diego, California 92138-9216.
J M
JM
Vaughan
W H
WH
Fischer
C
C
Hoeger
J
J
Rivier
W
W
Vale
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007028:Hypothalamic Hormones; D007031:Hypothalamus / Q000032:analysis*; D008543:Melanins / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010907:Pituitary Hormones / Q000302:isolation & purification*; D011863:Radioimmunoassay; D051381:Rats; D012474:Salmon; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; C019463:melanin-concentrating hormone