Separation of porphyrin isomers by high-performance liquid chromatography.
A high-speed reversed-phase high-performance liquid chromatographic method using an octadecylsilyl 3 cm long (3 microns particle size) column to separate the free acids of uroporphyrins I and III and coproporphyrins I and III from each other, and from the type I isomers of several other porphyrin carboxylic acids, is described. Separation of the porphyrins was achieved in less than 8 min, and injections were possible every 12 min. The detection limits of uroporphyrin, coproporphyrin, and mesoporphyrin were 75, 45, and 35 fmol (at a signal-to-noise ratio of 2), respectively. Application of the method to the determination of urinary and liver porphyrin patterns is shown.
Canadian Wildlife Service, National Wildlife Research Centre, Ottawa, Ontario.
S W
SW
Kennedy
A L
AL
Maslen
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000302:isolation & purification; D003370:Coturnix; D007536:Isomerism; D008099:Liver / Q000032:analysis; D011164:Porphyrias / Q000378:metabolism; D011166:Porphyrins / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D014578:Uroporphyrins / Q000302:isolation & purification
D003306:Coproporphyrins; D011166:Porphyrins; D014578:Uroporphyrins