Chromatographic analysis, isolation and partial characterization of two photocatalysed reaction products of haem and quinone.
Quinone was reacted in vitro with free haem under ultraviolet light. Two principal products were detected by liquid chromatography and purified by column chromatography. Mass spectral and Fourier transform infrared analyses showed that both products contained pyrroles and that the first product was probably an ether formed after reaction with the haem vinyl moieties. This product was entirely different from the known N-alkyl intermediates in the haem-catalysed epoxidation reactions of porphyrin dimerization by quinone.
Department of Industrial Hygiene and Toxicology, Institute of Occupational Health, Helsinki, Finland.
K
K
Peltonen
R
R
Kostiainen
H
H
Savolainen
D002384:Catalysis; D002853:Chromatography, Liquid; D005583:Fourier Analysis; D006418:Heme / Q000032:analysis*; D013058:Mass Spectrometry; D010777:Photochemistry; D011809:Quinones / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D011809:Quinones; D006418:Heme