Determination of 3,4-methylenedioxyamphetamine and 3,4-methylenedioxymethamphetamine enantiomers in whole blood.
A method for the determination of the enantiomeric content of 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) in microsamples (200 microliters) of whole blood is described. The method involves liquid-liquid extraction of MDA and MDMA from blood and derivatization with the chiral reagent N-trifluoroacetyl-L-prolyl chloride. Separation, identification and quantitation of diastereomeric derivatives is by gas chromatography-mass spectrometry. The analytical range of the assay is from 0.12 ng to 48 ng injected on-column. Details for the synthesis of the enantiomers of MDMA are also provided.
Fitzgerald
R L
RL
Department of Pharmacology and Toxicology, Medical College of Virginia, Richmond 23298.
Blanke
R V
RV
Glennon
R A
RA
Yousif
M Y
MY
Rosecrans
J A
JA
Poklis
A
A
D015104:3,4-Methylenedioxyamphetamine / Q000097:blood*; D000662:Amphetamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D013237:Stereoisomerism
D000662:Amphetamines; D015104:3,4-Methylenedioxyamphetamine