Technetium-99m haemaccel: a new lymphoscintigraphic agent.
Haemaccel, a denatured gelatin polymer with average molecular weight of 35,000, was labeled with Tc-99m and evaluated in rats and rabbits. The labeling efficiency was consistently higher than 85%. Tc-99m haemaccel (200 microCi in 0.05 ml) was injected intradermally into the hind footpads of rats lightly anaesthetized with diethyl ether vapour. Using a gamma camera, static images of the anterior view were obtained at various time intervals after injection. The images obtained demonstrated good localization of Tc-99m haemaccel in regional lymph nodes. In rats, about 3% of the injected dose was trapped in the node, and 48% remained at the injection site at 4 h after injection. In rabbits, good-quality images of lymph nodes and lymphatic channels of the hind legs were obtained within 15 min after intradermal injection of Tc-99m haemaccel. Tc-99m haemaccel showed fast migration from the injection site, good accumulation in the lymph nodes, and good visualization of lymphatic channels. Hence, it has a potential application in lymphoscintigraphy.
Nuclear Medicine Department, Faculty of Medicine, Kuwait University, Safat.
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Sadek
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Owunwanne
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Yacoub
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Abdel-Dayem
D000818:Animals; D003287:Contrast Media; D061305:Lymphoscintigraphy; D011097:Polygeline; D011108:Polymers; D011817:Rabbits; D051381:Rats; D013667:Technetium
D003287:Contrast Media; D011108:Polymers; D013667:Technetium; D011097:Polygeline