Effect of lithium on chemiluminescence of polymorphonuclear granulocytes and mononuclear cells of peripheral blood.
Influence of lithium on luminol amplified chemiluminescence activity of leucocytes of human peripheral blood was studied by a continuously recorded system. Lithium had a biphasic effect on mitogen-induced early activation of mononuclear cells, i.e. at concentration of 1.0 mM an increase, at higher concentrations a significant inhibition in photon emission was observed. Activating the mononuclear cells by opsonized Zymosan in the presence of lithium a dose-dependent decrease of chemiluminescence was registered. The respiratory burst of polymorphonuclear granulocytes induced by either mitogen or opsonized Zymosan was significantly inhibited at 2.0 and 5.0 mM of lithium, respectively. Chemiluminescence activity of peroxidase-dependent and independent cell-free system was not influenced by lithium. It was concluded that lithium by accumulating into target organs might have an immunosuppressive and antiphlogistic effect by mean of inhibition of antigen-presenting cells and polymorphonuclear granulocytes.
Department of Medicine, Teaching Hospital of Debrecen, Hungary.
C
C
Balázs
E
E
Kiss
N R
NR
Farid
D006098:Granulocytes / Q000187:drug effects*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008094:Lithium / Q000494:pharmacology*; D008163:Luminescent Measurements; D008213:Lymphocyte Activation / Q000187:drug effects; D010586:Phagocytes / Q000187:drug effects; D011961:Receptors, Fc / Q000276:immunology; D015054:Zymosan / Q000494:pharmacology
D011961:Receptors, Fc; D015054:Zymosan; D008094:Lithium