Xenobiotic microsomal epoxide hydrolase: 5' sequence of the human gene.
A cDNA for human microsomal epoxide hydrolase (mEH) was used to isolate genomic clones representing the 5' portion of the corresponding human gene. A total of 1034 bases of mEH gene sequence were obtained that included exon 1 and 5' flanking information. In contrast to the structure reported for the rat mEH gene (Falany et al. (1987) J. Biol. Chem. 262, 5924-5930), the human mEH gene lacked a CAAT box, but did contain a high-affinity CTF binding site at position -278 and an SV40 core enhancer element at position -525. TATA boxes were identified at positions -32 and -29 for the human and rat genes, respectively. Sequence comparisons between 5' flanking regions of the human and rat mEH genes demonstrated 47% homology, considerably less than the 83% conservation observed in the cDNA coding areas. Primer extension experiments localized the transcription initiation site of the human gene to a position 116 bases 5' from the analogous site in the rat mEH gene.
Wilson
N M
NM
Department of Environmental Health, School of Public Health and Community Medicine, University of Washington, Seattle 98195.
Omiecinski
C J
CJ
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004851:Epoxide Hydrolases / Q000235:genetics*; D006801:Humans; D008861:Microsomes / Q000201:enzymology*; D008969:Molecular Sequence Data; D014158:Transcription, Genetic; D015262:Xenobiotics / Q000493:pharmacokinetics*
D015262:Xenobiotics; D004247:DNA; D004851:Epoxide Hydrolases