Effect of antineoplastic drugs and estradiol on leucine uptake and proliferation of Ehrlich tumor cells.
Antineoplastic drugs (methotrexate, 5-fluorouracil, adriamycin) arrest the proliferation of Ehrlich ascites tumor cells in mice but have no influence on L-leucine uptake in vitro. Tamoxifen does not influence either process. Estradiol increases both the cellular proliferation and the amino acid uptake. The absence of estrogen receptors in the cells indicates that the hormone acts on cellular proliferation through mechanisms other than activation of DNA replication, such as stimulation of amino acid transport.
Department of Biochemistry, Faculty of Medicine, University of Buenos Aires, Argentina.
E
E
Levin
A
A
Cherñavsky
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005472:Fluorouracil / Q000494:pharmacology; D007930:Leucine / Q000493:pharmacokinetics*; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D013629:Tamoxifen / Q000494:pharmacology*
D000970:Antineoplastic Agents; D013629:Tamoxifen; D004958:Estradiol; D004317:Doxorubicin; D007930:Leucine; D005472:Fluorouracil; D008727:Methotrexate