Effect of alveolar hypoxia on pulmonary mast cells in vivo.
This study was undertaken to explore the effects of alveolar hypoxia on perivascular and periairway mast cell populations. Pulmonary mast cells were exposed to unilateral alveolar hypoxia by ventilating one lung of a cat with nitrogen. Mast cells from the contralateral lung, which was simultaneously ventilated with air, were used as a control. The granule content of perivascular and airway mast cells was determined from electron micrographs using morphometric methods. In response to alveolar hypoxia there was a 12% (p less than 0.005) decrease in the granule content of perivascular mast cells but no statistically significant change in periairway mast cell content. Perivascular mast cells from the hypoxic lungs did not show any of the morphological changes seen in IgE-mediated mast cell degranulation, such as granule swelling, fusion, or exocytosis. In the hypoxic lung, morphometric analysis revealed a significant decrease in the proportion of heterogeneous-appearing granules in the perivascular mast cells. The different reactivity of perivascular and periairway mast cells may explain why alveolar hypoxia does not induce significant bronchospasm.
Nadziejko
C E
CE
Department of Pathology, New York Medical College, Valhalla.
Loud
A V
AV
Kikkawa
Y
Y
D000818:Animals; D002415:Cats; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D000860:Hypoxia / Q000097:blood / Q000473:pathology* / Q000503:physiopathology; D008407:Mast Cells / Q000648:ultrastructure*; D010100:Oxygen / Q000097:blood; D011650:Pulmonary Alveoli / Q000473:pathology* / Q000503:physiopathology
D010100:Oxygen