Macrophage activation decreases macrophage prostaglandin E2 release in experimental trauma.
Prostaglandin E2 (PGE2) derived from macrophages following trauma may contribute to trauma-induced immunosuppression. This study evaluated the effect of glucan, a macrophage-activating agent, on macrophage PGE2 release in a murine trauma model. ICR/HSD mice were administered D5W, glucan pre-trauma, or glucan post-trauma, and subjected to hindlimb crush and amputation injury. Splenic macrophages were isolated 24 hours following trauma, cultured (24 hrs), and macrophage PGE2 levels were determined. In-vitro marrow proliferation was assessed as a measure of immune function. Crush-amputation injury increased (184%) macrophage PGE2 release. In contrast, glucan administration (pre or post) reduced PGE2 levels in macrophage supernatants (71% and 85%, respectively). A 52% decrease in in-vitro bone marrow proliferation was observed following trauma. Glucan pre- or post-trauma eliminated the suppression of bone marrow proliferation. In conclusion, macrophage-activating immunomodulators may exert beneficial effects following trauma by: 1) reducing macrophage PGE2 synthesis and release; and 2) reducing traumatic suppression of bone marrow proliferation.
Department of Surgery, Tulane University School of Medicine, New Orleans, Louisiana 70112.
H A
HA
Pretus
I W
IW
Browder
P
P
Lucore
R B
RB
McNamee
E L
EL
Jones
D L
DL
Williams
D000673:Amputation, Traumatic / Q000276:immunology* / Q000473:pathology; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D003444:Crush Syndrome / Q000276:immunology* / Q000473:pathology; D015232:Dinoprostone / Q000032:analysis / Q000096:biosynthesis*; D005936:Glucans / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011863:Radioimmunoassay; D012774:Shock, Traumatic / Q000276:immunology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013268:Stimulation, Chemical
D005936:Glucans; D015232:Dinoprostone