[The modifying action of methylmethane sulfonate on unscheduled DNA synthesis in the UV irradiation of human peripheral blood lymphocytes].
The value of the unscheduled DNA synthesis after the combined effect of UV radiation and methyl methanesulfonate (MMS) was considerably lower than that upon exposure to UV radiation alone and after two-hour incubation of the culture. These differences were insignificant after 26 h incubation. The result can be attributed to the alkylating effect of MMS on the repair DNA polymerase. With MMS delivered prior to UV irradiation there was an even larger decrease in the unscheduled DNA synthesis with both 2- and 26-hour incubation. The data obtained can be explained by the fact that MMS inhibits an excision endonuclease.
E I
EI
Genter
V M
VM
Mikhel'son
V D
VD
Zhestianikov
D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects* / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D013997:Time Factors; D014466:Ultraviolet Rays
D004247:DNA; D008741:Methyl Methanesulfonate
Modifitsiruiushchee deĭstvie metilmetansul'fonata na vneplanovyĭ sintez DNK pri UF-obluchenii limfotsitov perifericheskoĭ krovi cheloveka.