Testicular atrophy and loss of nerve growth factor-immunoreactive germ cell line in rats exposed to n-hexane and a protective effect of simultaneous exposure to toluene or xylene.
Testicular and germ cell line morphology in rats were studied 2 weeks, 10 months and 14 months after cessation of a 61-day inhalation exposure to 1000 ppm n-hexane. Androgen biosynthetic capacity of testis, testosterone blood concentration, vas deferens morphology and noradrenaline (NA) concentration, epididymal sperm morphology, and fertility were also studied. Severe testicular atrophy involving the seminiferous tubules with loss of the nerve growth factor (NGF) immunoreactive germ cell line was found. Total loss of the germ cell line was found in a fraction of animals up to 14 months post-exposure, indicating permanent testicular damage. No impairment of androgen synthesis or androgen dependent accessory organs was observed. Simultaneous administration of 1000 ppm n-hexane and 1000 ppm toluene, or 1000 ppm n-hexane and 1000 ppm xylene, did not cause germ cell line alterations or testicular atrophy. Toluene and xylene were thus found to protect from n-hexane induced testicular atrophy.
Department of Neuromedicine, National Institute of Occupational Health, Solna, Sweden.
P
P
Nylén
T
T
Ebendal
M
M
Eriksdotter-Nilsson
T
T
Hansson
A
A
Henschen
A C
AC
Johnson
T
T
Kronevi
U
U
Kvist
N O
NO
Sjöstrand
G
G
Höglund
D000818:Animals; D001284:Atrophy / Q000139:chemically induced; D005298:Fertility / Q000187:drug effects; D006586:Hexanes / Q000633:toxicity*; D007150:Immunohistochemistry; D008297:Male; D009414:Nerve Growth Factors / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013094:Spermatozoa / Q000276:immunology; D013737:Testis / Q000201:enzymology / Q000473:pathology*; D013739:Testosterone / Q000097:blood; D014050:Toluene / Q000494:pharmacology*; D014649:Vas Deferens / Q000166:cytology; D014992:Xylenes / Q000494:pharmacology*
D006586:Hexanes; D009414:Nerve Growth Factors; D014992:Xylenes; C026385:n-hexane; D014050:Toluene; D013739:Testosterone