Tissue specific distribution of calcyclin--10.5 kDa Ca2+-binding protein.
Expression of calcyclin in different cell lines and mouse tissues was determined with polyclonal antibodies raised against calcyclin from Ehrlich ascites tumour (EAT) cells. The protein was detected in mouse skeletal and cardiac muscle, in lung, kidney and spleen, and was especially enriched in mouse smooth muscle as well as in rat fibroblasts. No positive immunological reaction was detected in mouse brain, liver and intestine and some tumourigenic cell lines. The level of calcyclin mRNA found in different cells and tissues corresponded well to the calcyclin level estimated by immunoblotting. The calcyclin-like protein was purified from mouse stomach and appeared to be very similar to the EAT protein.
Nencki Institute of Experimental Biology, Warsaw, Poland.
J
J
Kuźnicki
A
A
Filipek
P
P
Heimann
L
L
Kaczmarek
B
B
Kamińska
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D018797:Cell Cycle Proteins; D002460:Cell Line; D004247:DNA / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods; D005347:Fibroblasts / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D009130:Muscle, Smooth / Q000032:analysis*; D009132:Muscles / Q000032:analysis*; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; D013270:Stomach / Q000032:analysis*
D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489134:S100a6 protein, mouse; C489132:S100a6 protein, rat; D004247:DNA