Production of hepatitis B virus surface antigen containing pre-S1 and pre-S2 domains by Chinese hamster ovary cells.
We have established transformed Chinese hamster ovary cells, which secrete hepatitis B virus surface antigen containing both pre-S1 and pre-S2 domains into culture medium, by using the autologous S gene promoter.
Lee
T
T
Kitasato Institute, Tokyo, Japan.
Inokoshi
J
J
Namiki
M
M
Takeshima
H
H
Omura
S
S
D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D010957:Plasmids; D011498:Protein Precursors / Q000096:biosynthesis*; D014759:Viral Envelope Proteins / Q000096:biosynthesis*; D014764:Viral Proteins / Q000096:biosynthesis / Q000648:ultrastructure
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen