Polymer support synthesis. XV. Behaviour of non-porous surface-coated silica gel microbeads in oligonucleotide synthesis.
Non-porous silica gel microbeads of diameter 1.5 microns have been investigated as supports for oligonucleotide synthesis. In the preparation of oligothymidylates of chain length up to 150 bases, with 5'-di-p-anisylphenylmethyl-3'-phosphoramidite as an intermediate, the average yields per chain elongation were up to 99%. Lower overall yields were observed in the case of a support which developed a strong tendency towards aggregation after the build up of an oligonucleotide coating.
Seliger
H
H
Universität Ulm, Sektion Polymere, F.R.G.
Kotschi
U
U
Scharpf
C
C
Martin
R
R
Eisenbeiss
F
F
Kinkel
J N
JN
Unger
K K
KK
D001345:Autoradiography; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011108:Polymers
D009841:Oligonucleotides; D011108:Polymers