Flash photolysis of the serum albumin-heme-CO complex.
Protoheme-CO in aqueous solution does not exhibit a geminate ligand recombination reaction. Addition of a protein, either globin or serum albumin, to which heme binds strongly, leads to an observable geminate reaction in aqueous solution. The bimolecular kinetic data for the albumin-heme-CO complex show two stable components, one heme-like in rate and difference spectrum, and one hemoglobin-like. The geminate reaction correlates spectrally with the hemoglobin-like component.
INSERM U299, Hôpital de Bicêtre, Le Kremlin Bicêtre, France.
M C
MC
Marden
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Hazard
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Leclerc
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QH
Gibson
D001665:Binding Sites; D002248:Carbon Monoxide / Q000528:radiation effects*; D006418:Heme / Q000528:radiation effects*; D007700:Kinetics; D010782:Photolysis; D012709:Serum Albumin / Q000528:radiation effects*; D013053:Spectrophotometry
D012709:Serum Albumin; D006418:Heme; D002248:Carbon Monoxide