Preparation of leukocyte-poor platelets by filtration.
There is evidence that leukocyte contaminating red blood cells and platelet concentrates are responsible for refractoriness to platelet transfusions. The efficacy of a cotton-wool filter to remove leukocytes from red blood cells has been documented previously. The present study was designed to evaluate whether the cotton-wool filters can effectively remove leukocytes from platelet concentrates. Sixty pools of random-donor platelets and single-donor plateletpheresis products were filtered through a cotton-wool filter. The efficacy of filtration was determined by measuring the absolute numbers of leukocytes and platelets and subpopulations of mononuclear cells. The average platelet loss was 8% per pool of random platelets and 10% per plateletpheresis product. The average leukocyte removal was 99% from a pool of random platelets and plateletpheresis concentrates collected by CS-3000 and 90% from plateletpheresis concentrates harvested by single-stage COBE/IBM-2997. The filtration removed 100% of granulocytes, 95% of monocytes, 90% of B-lymphocytes, and 85% of T-lymphocytes. We conclude that filtration through a cotton-wool filter is an efficient and cost-effective method for preparation of leukocyte-poor platelets.
Sniecinski
I
I
City of Hope National Medical Center, Blood Transfusion Services, Duarte, CA 91010.
Jean
J S
JS
Nowicki
B
B
D001781:Blood Component Removal / Q000379:methods*; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007958:Leukocyte Count; D008854:Microscopy, Electron; D010974:Platelet Aggregation; D010976:Platelet Count; D010983:Plateletpheresis / Q000379:methods*