[Stimulatory action of shakuyaku on aromatase activity in cultured rat follicles].
The direct action of herbal medicines on aromatase activity was investigated in cultured rat follicles. Mature follicles were obtained from PMSG-treated immature rats. Aromatase inhibitor, 4-hydroxy-4-androstene-3,17-dione (4-OHA) was also used in the experiment. Follicles were incubated in the medium for 24 hour with toki-shakuyaku-san, Keisi-bukuryo-gan. unkei-to (10-500 micrograms) or their ingredients (100 micrograms), respectively. The addition of 4-OHA reduced estradiol secretion in a dose-response manner. The addition of each medicine (100, 500 micrograms) as well as 4-OHA (10(-5) M) restored estradiol secretion to the control level. Among the ingredients, Shakuyaku stimulated estradiol secretion in the medium in the presence or absence of 4-OHA. It is noteworthy that shakuyaku alone had a stimulatory action on aromatase activity and is the only one which is equally contained in the three medicines.
Department of Obstetrics and Gynecology, Akita University School of Medicine.
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Ota
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Fukushima
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Maki
D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D003470:Culture Media; D046508:Culture Techniques; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006080:Ovarian Follicle / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051381:Rats
D003470:Culture Media; D004365:Drugs, Chinese Herbal; D004958:Estradiol; D001141:Aromatase