Deletions in a recombinant retrovirus genome associated with its expression in embryonal carcinoma cells.
We analyzed embryonal carcinoma cell lines infected with a recombinant Moloney murine leukemia virus. Lines that carried but did not express the neo gene retained a provirus of LTR-gag-pol-neo-LTR, where LTR is a long terminal repeat, whereas all G418-resistant lines deleted regions that included the primer binding site and the splicing donor site. This suggested the presence of multiple inhibitory elements.
Jackson Laboratory, Bar Harbor, Maine 04609.
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D002872:Chromosome Deletion; D004279:DNA, Viral / Q000032:analysis; D054278:Embryonal Carcinoma Stem Cells; D005814:Genes, Viral; D008979:Moloney murine leukemia virus / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000382:microbiology*; D011533:Proviruses / Q000235:genetics; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
D004279:DNA, Viral