Heterogeneity of hematopoietic cells immortalized by v-myc/v-raf recombinant retrovirus infection of bone marrow or fetal liver.
The J2 recombinant retrovirus expressing v-myc/v-raf (also known as MYC/RAF1) immortalized macrophages from the bone marrow of lipopolysaccharide-responsive mouse strains, producing the ANA-1 cell line from C57BL/6 mice and the INF-3A cell line from C3H/HeN mice. In contrast, J2 recombinant retrovirus infection of the fetal liver from C57BL/6-Ly-5a mice immortalized a cell line (GGD) that did not exhibit the characteristics of mature macrophages. The GGD cell line was classified as leukocytic on the basis of its expression of the Ly-6B.2, Fc gamma R, and Ly-5.2 antigens. Our results indicate that the J2 recombinant retrovirus selectively immortalizes macrophages from the bone marrow of C57BL/6 and C3H/HeN mice but immortalizes cells without definitive macrophage characteristics from murine fetal liver under the same culture conditions.
Laboratory of Molecular Immunoregulation, NCI-Frederick Cancer Research Facility, MD 21701.
G W
GW
Cox
B J
BJ
Mathieson
L
L
Gandino
E
E
Blasi
D
D
Radzioch
L
L
Varesio
D000818:Animals; D001854:Bone Marrow Cells; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D008099:Liver / Q000166:cytology / Q000196:embryology; D008264:Macrophages / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009857:Oncogenes; D010587:Phagocytosis; D010641:Phenotype; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology