Ultraviolet light-resistant primary transfectants of xeroderma pigmentosum cells are also DNA repair-proficient.
In a previous work, an immortal xeroderma pigmentosum cell line belonging to complementation group C was complemented to a UV-resistant phenotype by transfection with a human cDNA clone library. We now report that the primary transformants selected for UV-resistance also acquired normal levels of DNA repair. This was assessed both by measurement of UV-induced [3H]thymidine incorporation and by equilibrium sedimentation analysis of repair-DNA synthesis. Therefore, the transduced DNA element which confers normal UV-resistance also corrects the excision repair defect of the xeroderma pigmentosum group C cell line.
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
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Stark
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Naiman
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Canaani
D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D004260:DNA Repair; D006801:Humans; D066298:In Vitro Techniques; D014162:Transfection; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics*