Synthesis and analysis of selenomethionine metabolites.
This paper describes the enzymatic synthesis of selenomethionine metabolites of the transmethylation and polyamine synthesis pathways: adenosylselenomethionine, adenosylselenohomocysteine, decarboxylated adenosylselenomethionine, and methylselenoadenosine. These compounds and the corresponding methionine metabolites were simultaneously separated by a single HPLC run. The sensitivity of the HPLC method is about 20 pmol per compound. The method may be used for direct analysis of the metabolite levels in tissues or cells treated with selenomethionine and it provides an assay method for the pulse-chase type of analysis of relative flows for both selenium- and sulfur-containing compounds in transmethylation and polyamine pathways.
Department of Biochemistry, University of Kuopio, Finland.
E O
EO
Kajander
R L
RL
Pajula
R J
RJ
Harvima
T O
TO
Eloranta
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012643:Selenium / Q000138:chemical synthesis*; D012645:Selenomethionine / Q000031:analogs & derivatives / Q000032:analysis / Q000138:chemical synthesis*; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured
D012645:Selenomethionine; D012643:Selenium