New antitumor monoclonal antibody-vinca conjugates LY203725 and related compounds: design, preparation, and representative in vivo activity.
A method has been developed to allow the direct coupling of the cytotoxic vinca alkaloid 4-desacetylvinblastine-3-carbohydrazide (DAVLB hydrazide) to a variety of murine monoclonal antibodies directed against human solid tumors. Periodate oxidation of carbohydrate residues on the antibodies, followed by reaction with DAVLB hydrazide in aqueous acid affords, in most cases, conjugates with conjugation ratios of 4-6 vincas per antibody in high yield without significantly impairing antigen binding or solubility. The outcome of the conjugation reaction is highly dependent on the concentration of, and time of exposure of the protein to, the oxidant. These conjugates exhibit potent antitumor activity in vivo against a number of human solid tumor-nude mouse xenografts, with efficacy and safety increased over unconjugated DAVLB hydrazide. This antitumor activity is also superior to that of similarly prepared but nontarget tumor binding antibody-DAVLB hydrazide conjugates. MoAb-DAVLB hydrazide conjugates release DAVLB hydrazide in solution in a temperature- and pH-dependent manner. Hydrolytic release of unmodified DAVLB hydrazide from tumor-localized MoAb-DAVLB hydrazide conjugates in vivo may be an important factor in their antitumor activity.
Laguzza
B C
BC
Lilly Research Laboratories, Indianapolis, Indiana 46285.
Nichols
C L
CL
Briggs
S L
SL
Cullinan
G J
GJ
Johnson
D A
DA
Starling
J J
JJ
Baker
A L
AL
Bumol
T F
TF
Corvalan
J R
JR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000138:chemical synthesis* / Q000627:therapeutic use; D015195:Drug Design; D006801:Humans; D000922:Immunotoxins / Q000138:chemical synthesis* / Q000627:therapeutic use; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000188:drug therapy; D013329:Structure-Activity Relationship; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000627:therapeutic use
C055183:4-desacetylvinblastine hydrazide-monoclonal antibody KS1-4 conjugate; D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D014747:Vinblastine