Enhancement of the adherence of hematopoietic stem cells to mouse bone marrow-derived stromal cell line MS-1-T by a tetrapeptide acetyl-N-Ser-Asp-Lys-Pro.
The tetrapeptide acetyl-N-Ser-Asp-Lys-Pro (AcSDKP) has been shown to inhibit in vivo the hematopoietic stem cell (spleen colony-forming unit; CFU-S) entry into cell cycle in cytosine arabinoside-treated mice. Our data showed that AcSDKP has no effect on interleukin 3 (IL-3)-dependent DA-1 cell proliferation or on granulocyte-macrophage colony-forming cell and mast cell colony formation, whereas it enhances the adherence of CFU-S to the bone marrow-derived hematopoietic supportive stromal cell line MS-1-T. AcSDKP suppresses MS-1-T proliferation but does not modify granulocyte-macrophage colony-stimulating activity secretion by these cells. This suggests that the peptide does not counteract the activity of the IL-3 receptor on CFU-S but acts on MS-1-T and in particular at the level of CFU-S/MS-1-T interactions.
ICSN/CNRS, Gif-sur-Yvette, France.
M
M
Lenfant
K
K
Itoh
H
H
Sakoda
D
D
Sotty
N A
NA
Sasaki
J
J
Wdzieczak-Bakala
K J
KJ
Mori
D000818:Animals; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D008264:Macrophages / Q000166:cytology; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*
D009842:Oligopeptides; C058504:goralatide