Quantitation of microtubule-associated protein MAP-1B in brain and other tissues.
1. The presence of microtubule-associated protein MAP-1B in all mammalian tissues tested, as well as in brain, has been demonstrated by immunoblotting using a monospecific polyclonal antibody. 2. The expression of brain MAP-1B is developmentally controlled, as it is less abundant in adult than in newborn rat brain, where it is a major microtubule assembly promoting factor. 3. The level of MAP-1B in tissues other than brain is lower than it is in brain; but the relative ratios of MAP-1B to tubulin are very similar in all tissues, thus differing from the observed for MAP-2 or tau. 4. The amount of MAP-1B in non-nervous tissues seems not to be under developmental control. 5. These results are consistent with a role for MAP-1B in the assembly of microtubules in most cells.
Díaz-Nido
J
J
Centro de Biologia Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Avila
J
J
D000311:Adrenal Glands / Q000032:analysis; D000818:Animals; D001921:Brain / Q000254:growth & development; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008168:Lung / Q000032:analysis; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D010179:Pancreas / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D008869:Microtubule-Associated Proteins