[Comparison of promoting the proliferation and fusion of myoblast between conditioned media from fibroblasts of chick embryo and fetal mouse].
Conditioned media were prepared by using the collected media which had cultured fibroblasts from muscle tissue of fetal mouse (ICR) or chick embryo (Leghorn chicken). The effects of these media on the proliferation and fusion in mouse or chick myoblast were studied quantitatively. The results were as follows: (1) the conditioned medium from fibroblasts of fetal mouse promoted the proliferation of mouse or chick embryonic myoblasts by 2.65 times (P less than 0.001) or 2.35 times (P less than 0.01) as compared with control groups respectively. (2) The conditioned medium from fibroblasts of embryonic chicken promoted the proliferation of chick or mouse embryonic myoblasts by 2.66 times (P less than 0.01) or 2.17 times (P less than 0.01) respectively. (3) The conditioned medium from fibroblasts of fetal mouse enhanced the fused rate of mouse or chick myoblasts by 1.9 or 2.6 times respectively. The conditioned medium from fibroblasts of embryonic chick enhanced the rate of chick myoblast fusion by 2.1 times, but the effect on the mouse myoblast fusion was not remarkable. The results suggest that the effect of fibroblast conditioned media on myoblast proliferation is similar in the two species, but the effect on the fusion of myoblasts is somewhat species-specific.
J F
JF
Gu
Y Q
YQ
Yan
Z H
ZH
Zhou
D000818:Animals; D002455:Cell Division; D002459:Cell Fusion; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D008813:Mice, Inbred ICR; D009132:Muscles / Q000166:cytology*
D003470:Culture Media