Expression of cytoplasmic granules with T cell-associated serine proteinase-1 activity in Ly-2+(CD8+) T lymphocytes responding to lymphocytic choriomeningitis virus in vivo.
Monoclonal antibodies (mAb) with specificity for the T cell-associated serine proteinase-1 of the mouse (MTSP-1) were used to study expression and storage of this enzyme in T lymphocytes during lymphocytic choriomeningitis (LCM) virus infection in vivo. Immunohistochemical analysis of splenic tissue at the peak of LCM virus-specific T cell-mediated cytolytic responses, i.e., at day 7 post infection, revealed high numbers of MTSP-1+ T lymphocytes in the interfollicular T cell-dependent area of the spleen. More than 50% of Ly-2+(CD8+) cells but only low numbers of Ly-2-(CD8-) cells, previously enriched by flow cytofluorometry, contained large amounts of cytoplasmic granules which stained both with MTSP-1-specific mAb and the esterase substrate N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl ester. These data demonstrate that in vivo generated LCM virus-induced cytolytic T lymphocytes develop cytoplasmic storage granules containing MTSP-1 and suggest that the mechanism of granule exocytosis is operative in vivo; possibly MTSP-1 is one effector molecule participating in the Ly-2+(CD8+) T lymphocyte-mediated control of virus infection.
Universitäts-Hautklinik, Heidelberg, FRG.
M D
MD
Kramer
U
U
Fruth
H G
HG
Simon
M M
MM
Simon
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D003594:Cytoplasmic Granules / Q000201:enzymology*; D005260:Female; D007150:Immunohistochemistry; D008216:Lymphocytic Choriomeningitis / Q000201:enzymology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D012697:Serine Endopeptidases / Q000276:immunology / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D012697:Serine Endopeptidases