[A recommended method for determination of uric acid in serum].
SFBC work group on uric acid propose a method for evaluation in blood serum which is modeled on the one defined by American Association for Clinical Chemistry. Serum is deproteinized by trichoracetic acid and the supernatant, buffered to pH 8.5, is submitted to uricase action. The disappearing of the strong band of uric acid in UV is measured by derivative spectrophotometry; this procedure vanished the effect of trouble which remains in the supernatant. This spectrophotometric technic permit to reach, in multiple sites, a variation coefficient near of 1 p. cent.
Hôpital J. Courmont, CHU Lyon-Sud, Pierre-Bénite.
P
P
Baltassat
D
D
Brault
M
M
Cambillau
J F
JF
Demelier
D
D
Duchassaing
P
P
Levillain
B
B
Mousson
F
F
Paolaggi
D
D
Porquet
P
P
Laruelle
D000704:Analysis of Variance; D013053:Spectrophotometry / Q000379:methods; D014238:Trichloroacetic Acid; D014503:Urate Oxidase; D014527:Uric Acid / Q000097:blood*
D014527:Uric Acid; D014238:Trichloroacetic Acid; D014503:Urate Oxidase
Méthode recommandée pour le dosage de l'acide urique dans le sérum.