Presence of multiple species of polypeptides immunologically related to transcription factor TFIIIA in adult Xenopus tissues.
Transcription of 5S RNA gene in Xenopus oocytes requires a 38 kDa transcription factor TFIIIA, which interacts with the 50 bp internal control region of the gene. We looked for TFIIIA-like polypeptides in the extracts of adult Xenopus tissues on the basis of their antigenic cross-reactivity to anti-TFIIIA antibody. Several species of polypeptides ranging from 30 to 50 kDa were found in kidney, stomach, liver and testis. Although these polypeptides reacted specifically to anti-TFIIIA antibody, proteolytic peptide mapping of three representative ones did not reveal any mutual similarities. They also seemed to be distinct from TFIIIA. Possible functions of these proteins are discussed.
Department of Molecular and Experimental Medicine, Scripps Clinic and Research Foundation, La Jolla, CA 92037.
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Yasui
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Ryoji
D000818:Animals; D000906:Antibodies; D003488:Cyanogen Bromide; D005260:Female; D015151:Immunoblotting; D007074:Immunoglobulin G; D008297:Male; D008970:Molecular Weight; D009865:Oocytes / Q000032:analysis; D009928:Organ Specificity; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification*; D012341:RNA, Ribosomal, 5S; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000276:immunology / Q000302:isolation & purification*; D014982:Xenopus laevis
D000906:Antibodies; D007074:Immunoglobulin G; D010446:Peptide Fragments; D010455:Peptides; D012341:RNA, Ribosomal, 5S; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors; D003488:Cyanogen Bromide