Electron microscopic studies of viruses labeled with magnetite.
We were able to develop a method with which to successfully and specifically detect virus particles under the electron microscope by using magnetite. This method was devised on the principle that magnetite-labeled antibody or magnetite coupled with protein A selectively bind virus or antibody-treated virus particles on the electron microscope grid by the action of an electromagnet. Another advantage characterizing the technique is the possibility of detection of a small number of virus particles. This is done through a process of concentration and purification of the reaction complexes trapped rigidly by magnetic force.
Department of Virology, Kanto-Teishin Hospital, Tokyo.
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Mizutani
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Mizutani
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Nozaki
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Fujiwara
D000914:Antibodies, Viral / Q000276:immunology; D000915:Antibody Affinity; D052203:Ferrosoferric Oxide; D006801:Humans; D007501:Iron; D009975:Orthomyxoviridae / Q000276:immunology / Q000648:ultrastructure; D010087:Oxides; D014771:Virion / Q000648:ultrastructure*
D000914:Antibodies, Viral; D010087:Oxides; D007501:Iron; D052203:Ferrosoferric Oxide