Hexanedione effects on protein phosphorylation in rat peripheral nerve.
Rats were treated with either 2,5-hexanedione (2,5-HD), 1,6-hexanediol (1,6-HDIOL), or saline for 7, 15 or 24 days. Protein phosphorylation was measured in proximal and distal sciatic nerve segments following incubation with [32P]orthophosphate. In proximal segments, 2,5-HD administration caused selective time-dependent increases in isotope incorporation in a 55 kDa protein, tentatively identified as tubulin, and a 180 kDa protein. Enhanced phosphorylation was highest at 24 days when motor function was most impaired. Administration of 1,6-HDIOL produced no consistent phosphorylation changes. Animals intoxicated with 3,4-dimethyl-2,5-hexanedione for 12 days showed proximal region increases in phosphorylation of the 55 and 180 kDa proteins and the major myelin proteins, Po and Pr.
Department of Pharmacology, University of Houston, TX 77004.
K L
KL
Horan
J
J
Eichberg
L N
LN
Berti-Mattera
R M
RM
LoPachin
D000818:Animals; D006588:Hexanones; D008297:Male; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism*
D006588:Hexanones; D009419:Nerve Tissue Proteins; C039195:3,4-dimethyl-2,5-hexanedione