The bioenergetics of mitochondria after cryopreservation.
The functional characteristics of rat liver mitochondria after cryopreservation with and without the addition of the cryoprotectant dimethyl sulfoxide (Me2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me2SO effected some changes in mitochondrial function.
Academic Department of Surgery, Royal Free Hospital School of Medicine, University of London, England.
B J
BJ
Fuller
A
A
Rubinacci
K
K
Geboes
W
W
De Loecker
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D004121:Dimethyl Sulfoxide; D004734:Energy Metabolism; D005615:Freezing; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000378:metabolism; D010101:Oxygen Consumption; D051381:Rats; D014021:Tissue Preservation
D000255:Adenosine Triphosphate; D004121:Dimethyl Sulfoxide