A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth.
Laminin is a basement membrane glycoprotein which consists of A, B1, and B2 chains. Laminin has diverse biological activities including promoting cell adhesion, migration, differentiation, growth, and neurite extension. Synthetic peptides from the active region of the A chain were prepared and tested for their biological activity. A 19-mer peptide (designated PA22-2), from just above the carboxyl globule on the long arm of the A chain, was found to promote cell adhesion, spreading, migration, and neurite outgrowth. By testing smaller sequences within the 19-mer peptide, a constituent pentapeptide, IKVAV (Ile-Lys-Val-Ala-Val), was identified as the active site for cell adhesion and neurite outgrowth. These data suggest that this sequence is one of the principle sites in laminin which regulate cellular behavior.
Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, Bethesda, Maryland 20892.
K
K
Tashiro
G C
GC
Sephel
B
B
Weeks
M
M
Sasaki
G R
GR
Martin
H K
HK
Kleinman
Y
Y
Yamada
D000310:Adrenal Gland Neoplasms; D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D002633:Chemotaxis / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D007797:Laminin / Q000138:chemical synthesis / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D010673:Pheochromocytoma; D051381:Rats; D013329:Structure-Activity Relationship
D007797:Laminin; D009842:Oligopeptides