Full length cDNA structure and deduced amino acid sequence of human 3 beta-hydroxy-5-ene steroid dehydrogenase.
Polyclonal antibodies raised against 3 beta-hydroxysteroid dehydrogenase isolated from human placenta were used to screen a lambda gt11 expression cDNA library from the same tissue. The protein deduced from cDNA sequences contains 372 amino acids with a calculated mol wt of 42,216. Since 3 beta-hydroxysteroid dehydrogenase is the enzyme catalyzing the formation of all classes of hormonal steroids, the availability of the cDNA encoding this enzyme opens new possibilities for a detailed investigation of the factors regulating the expression and activity of this crucial enzyme in adrenal, gonadal as well as peripheral tissues.
Luu The
V
V
Department of Molecular Endocrinology, Laval University Medical Centre, Quebec, Canada.
Lachance
Y
Y
Labrie
C
C
Leblanc
G
G
Thomas
J L
JL
Strickler
R C
RC
Labrie
F
F
D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000906:Antibodies; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D000906:Antibodies; D015096:3-Hydroxysteroid Dehydrogenases; C031954:delta(5)-3 beta-hydroxysteroid dehydrogenase