[Construction of an apparatus for pulsed field electrophoresis for the analysis of high molecular weight DNA].
An inexpensive apparatus for Pulsed Field Gel Electrophoresis constructed by the Authors, is described. According to our experimental condition it was found that this apparatus is capable to resolve DNA fragments in order of 50-1500 Kb. This range of analysis is adequate for the molecular studies adjacent regions of prokaryotes, primitive eukaryotes and mammals.
M
M
Gennarelli
G
G
Novelli
A
A
Ruzzo
F
F
Grianti
B
B
Dallapiccola
D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation*; D004867:Equipment Design; D008970:Molecular Weight
D004247:DNA
Realizzazione di un apparecchio per elettroforesi a campi pulsanti per analisi di DNA ad alto peso molecolare.