Induction of rat liver glutathione transferase subunit 7 by lead nitrate.
The effect of a single dose of lead nitrate (10 microM/100 g body wt), a hepatic mitogen, on rat liver glutathione transferase (GST) subunit expression was investigated. Using SDS-polyacrylamide gel electrophoresis and Western blot technique evidence for the induction of GST 7-7 is shown. This occurrence is identical to that observed in preneoplastic nodules generated in rat liver by different models of chemical carcinogenesis, suggesting that lead nitrate may be a very simple model for investigation of the mechanism of glutathione transferase 7-7 gene expression in chemical hepatocarcinogenesis.
Istituto di Scienze Biochimiche, Facolta di Medicina, Universita G. D'Annunzio Chieti, Italy.
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Di Ilio
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Aceto
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Columbano
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Ledda-Columbano
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Federici
D000818:Animals; D015153:Blotting, Western; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D004976:Ethacrynic Acid / Q000378:metabolism; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000235:genetics; D007854:Lead / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009566:Nitrates / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D004137:Dinitrochlorobenzene; D009566:Nitrates; D007854:Lead; C017461:lead nitrate; D005982:Glutathione Transferase; D004976:Ethacrynic Acid