[Nucleotide sequence of intergenic and external transcribed spacers of rDNA of diploid wheat Triticum urartu Thum. ex Candil].
The primary structure of intergenic non-transcribed and external transcribed spacers of rDNA of diploid wheat Triticum urartu, cloned in pTu3 plasmid 2402 b.p. long was determined. The intergenic non-transcribed rDNA spacer of Tr. urartu was shown to consist of 8 subrepeats with an average of 133 b.p. long, heterogeneous in length and nucleotide sequence. A number of repeated sequences was revealed within each subrepeat. While comparing nucleotide sequences of rDNA subrepeats of Tr. urartu and Tr. aestivum a high homology was found (up to 82%). A high similarity between these plant species was also found in the promoter region and in the external transcribed rDNA spacer. Suppression of the nucleolar organizer of 1A chromosome in the presence of 1B and 6B chromosomes of Tr. aestivum is supposed to be connected with the existence of a great number of subrepeats in the intergenic non-transcribed rDNA spacer of B genome donors in polyploid wheat species of turgidum-aestivum row.
Vakhitov
V A
VA
Chemeris
A V
AV
Akhmetzianov
A A
AA
D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D004171:Diploidy; D008969:Molecular Sequence Data; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics*
D004275:DNA, Ribosomal
Nukleotidnaia posledovatel'nost' mezhgennogo i vneshnego transkribiruemogo speĭserov rDNK diploidnoĭ pshenitsy Triticum urartu Thum. ex candil.