4-Bromophenacyl bromide inhibits prostaglandin D2 synthesis from arachidonic acid rather than phospholipase A2 activity in liver macrophages.
4-Bromophenacyl bromide at a concentration of 50 microM does not inhibit phospholipase A2 activity in liver macrophages. Rather, this compound increases the amount of radioactivity released from [3H]arachidonate-prelabeled Kupffer cells and leads to the formation of small amounts of thromboxane, prostaglandin D2 and prostaglandin E2. Also the zymosan-induced formation of thromboxane and prostaglandin E2 from endogenous sources which is thought to involve phospholipase A2 remains unaffected in the presence of this compound. The generation of superoxide and the formation of prostaglandin D2 from arachidonate and after stimulation of the cells with zymosan, however, are blocked by 4-bromophenacyl bromide. Furthermore, this compound suppresses the incorporation of externally added arachidonate into membrane lipids of the cells. 4-Bromophenacyl bromide seems, therefore, not to be a useful tool to demonstrate the involvement of phospholipase A2 in complex biological systems.
Biochemisches Institut, Albert-Ludwigs-Universität, Freiburg i.Br.
P
P
Dieter
A
A
Schulze-Specking
K
K
Decker
D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D007728:Kupffer Cells / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D010740:Phospholipases / Q000502:physiology*; D010741:Phospholipases A / Q000502:physiology*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D015230:Prostaglandin D2 / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011863:Radioimmunoassay; D051381:Rats; D013929:Thromboxane B2 / Q000096:biosynthesis; D015054:Zymosan / Q000494:pharmacology
D000098:Acetophenones; D001095:Arachidonic Acids; D008563:Membrane Lipids; D010743:Phospholipids; D011458:Prostaglandins E; D013929:Thromboxane B2; D015054:Zymosan; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; C006821:4-bromophenacyl bromide; D015230:Prostaglandin D2