On the metabolism of GM3 ganglioside in cultured human foreskin fibroblasts.
The metabolism of GM3 ganglioside in cultured human foreskin fibroblasts was investigated by labeling cultured cells with [1-3H]-galactose for 48 hours, followed by a 48 hour chase. More than 80% of the radioactivity associated with GM3 was found in the hexose portion of the carbohydrate chain, whereas approximately 12% of the radioactivity was observed in the sialic acid moiety. The hexose and sialic acid residues lost 42% and 53% of their initial radioactivity, respectively, during the chase period, indicating an active metabolism of these sugar residues of GM3 in growing cultures.
Department of Biochemistry, Michigan State University East Lansing 48824-1319.
M J
MJ
Swamy
C C
CC
Sweeley
D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D005679:G(M3) Ganglioside / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008297:Male; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000378:metabolism
D005679:G(M3) Ganglioside; D005732:Gangliosides; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid; D005947:Glucose; D005690:Galactose