Thermally adaptive changes of mycolic acids in Mycobacterium smegmatis.
The effect of growth temperature on mycolic acid composition in eight strains of Mycobacterium smegmatis was investigated by gas chromatography/mass spectrometry. A change in growth temperature from 45 to 20 degrees C caused a shift in the subclass and molecular species composition of mycolic acids. The relative amount of alpha'-mycolic acids to alpha-mycolic acids decreased, and that of hydroxy mycolic acids increased at lower temperatures. Moreover, the proportion of shorter-chain species of alpha-mycolic acids increased, and those of longer-chain species of alpha-mycolic and hydroxy mycolic acids decreased. This observation seems to be due to the changes of the chain length of meromycolates because the alpha-alkyl chain unit of mycolic acids was not affected. The ratio of odd to even carbon-numbered alpha-mycolates decreased as the growth temperature was lowered. In contrast, the molecular species composition of alpha'-mycolic acid was not influenced by the growth temperature.
Baba
T
T
Department of Domestic Science, Shoin Women's College, Hyogo.
Kaneda
K
K
Kusunose
E
E
Kusunose
M
M
Yano
I
I
D000222:Adaptation, Physiological; D000818:Animals; D002455:Cell Division; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D009161:Mycobacterium / Q000378:metabolism; D009171:Mycolic Acids / Q000096:biosynthesis* / Q000145:classification; D013696:Temperature
D009171:Mycolic Acids