[Histological take rates of fresh human tumors in the subrenal capsular space of cyclosporin A treated mice].
To elucidate the reliability of subrenal capsule assay, histological take rates of fresh human tumors from 75 clinical cases were studied. (1) Take rates of the clinical materials in immune suppressed ddY mice treated by cyclosporin A (60 mg/kg sc, qd) at 6 day were 71% in colon cancer, 50% in stomach cancer, 43% in breast cancer and 0% in lymphoma. (2) Regarding to the histological type, take rates were better in more differentiated type being around 80%, and worse in poorly differentiated type, around 20%. (3) Factors affecting on the poor take rates, other than organ type and histological type, were such as strong heterogeneity of fragments, paucity of cancer cells and abundant stroma. Good tumor take among poorly differentiated type was observed in medullary type. (4) By the daily observation of the growing pattern of the implants (mainly assessed by the amounts of viable tumor cells) the group with good tumor shows similar number of cells through the experimental days and, on the other hand, group with poor tumor shows an early disappearance of tumor cells at day 1 or 2. (5) By lowering pH of the medium harboring fragments down to pH 6.8-7.0 take rates were improved as well as the quality of viability.
Dept. of Surgery, National Fukuoka Central Hospital.
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Yamada
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology; D003470:Culture Media; D003524:Cyclosporins / Q000494:pharmacology*; D005260:Female; D006085:Graft Survival; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology; D013370:Subrenal Capsule Assay
D003470:Culture Media; D003524:Cyclosporins