Dot-enzyme-linked immunosorbent assay (dot-ELISA) for the rapid diagnosis of human fascioliasis.
A dot-enzyme-linked immunosorbent assay (dot-ELISA) was developed as a fast and field applicable antibody detection tool for the diagnosis of human fascioliasis. The assay is performed using partially purified antigens from a species of Fasciola at 180 ng protein/dot (2 microliters) and serum samples at 1:20 dilution (1 microliter). Dot-ELISA results completely agreed with those of micro-ELISA. Antigen-coated nitrocellulose sheets stored for 3 mo at -20 C showed results identical to fresh sheets. Sera from patients with fascioliasis (n = 30) and other parasitic or viral infections (n = 120) were compared with sera from healthy controls (n = 14). Ninety samples can be tested within 90 min. The sensitivity, specificity, and speed of the assay may justify its use in laboratory and field studies.
Shaheen
H I
HI
U.S. Naval Medical Research Unit No. 3, Cairo, Egypt, F.P.O., New York 09527.
Kamal
K A
KA
Farid
Z
Z
Mansour
N
N
Boctor
F N
FN
Woody
J N
JN
D000947:Antigens, Helminth / Q000032:analysis; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005211:Fascioliasis / Q000175:diagnosis* / Q000276:immunology; D005243:Feces / Q000469:parasitology; D006801:Humans
D000947:Antigens, Helminth