[Determination of liver cholesterol-7 alpha-monooxygenase activity in an in vivo experiment].
The method of determination of cholesterol-7 alpha-monooxygenase activity in vivo which has a number of advantages over the existing ones is offered. [3H]cholesterin was injected into rats intravenously in the form of albumin-stabilized emulsion. In 2--4 h and then every day 3H2O radioactivity of one of the cholesterin enzymatic hydroxylation products in microsomal liver apparatus was estimated in animals' urine. The dynamics of 3H2O secretion during 7 days and dependence of the urine radioactivity upon the given radionuclide dose are shown.
V P
VP
Tverdokhlib
A A
AA
Nikonorov
V K
VK
Filippov
B A
BA
Frolov
S N
SN
Afonina
D000818:Animals; D002784:Cholesterol / Q000008:administration & dosage / Q000652:urine; D002790:Cholesterol 7-alpha-Hydroxylase / Q000032:analysis* / Q000378:metabolism; D008722:Methods; D008862:Microsomes, Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats; D013250:Steroid Hydroxylases / Q000032:analysis*; D014316:Tritium
D014316:Tritium; D002784:Cholesterol; D013250:Steroid Hydroxylases; D002790:Cholesterol 7-alpha-Hydroxylase
Opredelenie aktivnosti kholesterol-7 al'fa-monooksigenazy pecheni v éksperimente in vivo.