Comparison of human normal, full-term, fetal and adult plasminogen by physical and chemical analyses.
Human fetal plasminogen was isolated from fetal cord blood obtained from full-term normal newborns. Two fetal plasminogen preparations were characterized by physical analyses and compared to adult human Glu-plasminogen. The protein concentration of plasminogen in each full-term fetal plasma was approximately 50% of the concentration found in adult plasma. The specific activity of the isolated plasminogen from both full-term fetal plasmas was 28.8 +/- 1.5 IU/mg protein, approximately the same as that of adult Glu-plasminogen. No significant difference was observed in the rate at which plasmin was generated from the normal fetal plasminogen and the adult Glu-plasminogen using streptokinase, urokinase, and tissue plasminogen activator. Electrophoretic analyses in an acrylamide gel/sodium dodecyl sulfate dissociating system showed that the fetal plasminogens and the adult Glu-plasminogen were the same molecular size. Analyses in an acrylamide gel isoelectric focusing system indicated that fetal and adult plasminogen both contained the same twelve isoelectric forms, however, there was a slight difference in the distribution of the isoelectric forms. The fetal and adult plasminogens both contained 792 +/- 1 amino acid residues, and there were no significant differences in amino acid composition between the fetal and adult preparations. These comparisons indicate that normal, full-term, fetal and adult Glu-plasminogen are identical.
Department of Surgery, Evanston Hospital, Ill.
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Summaria
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D001797:Blood Protein Electrophoresis; D005326:Fetal Proteins / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn / Q000097:blood; D007525:Isoelectric Focusing; D010958:Plasminogen / Q000032:analysis*
D000596:Amino Acids; D005326:Fetal Proteins; D010958:Plasminogen