Stabilization of actin filaments by ATP and inorganic phosphate.
Both inorganic orthophosphate and ATP stabilize actin filaments. This is reflected by a reduced nucleotide exchange and by a protection against filament breakdown by SDS or KI. When the filament-stabilizing effect of ATP was maximal, only about 15% of the actin subunits of the filament had bound one molecule of the nucleotide offered in the stabilizing solution.
Institut für Zoologie der Technischen Hochschule Darmstadt, Bundesrepublik Deutschland.
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D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000266:Adenylyl Imidodiphosphate / Q000494:pharmacology; D002250:Carbon Radioisotopes; D007700:Kinetics; D046911:Macromolecular Substances; D010710:Phosphates / Q000378:metabolism*; D011193:Potassium Iodide / Q000494:pharmacology; D011485:Protein Binding; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D000199:Actins; D002250:Carbon Radioisotopes; D046911:Macromolecular Substances; D010710:Phosphates; D011193:Potassium Iodide; D000266:Adenylyl Imidodiphosphate; D012967:Sodium Dodecyl Sulfate; D000255:Adenosine Triphosphate