Isolation of human cDNA clones of ski and the ski-related gene, sno.
cDNA clones of ski and the ski-related gene, sno, were obtained by screening human cDNA libraries. The predicted open reading frame of h-ski could encode a protein of 728 amino acid residues. The h-ski protein is highly homologous with the v-ski protein. The overall homology between h-ski and v-ski is 91% at the amino acid level. DNA sequencing analysis revealed two types of cDNA clones from the sno (ski-related novel gene) gene, possibly due to alternative splicing. The first type, named snoN (non Alu-containing), encoded a protein of 684 amino acid residues. The second type, named snoA (Alu-containing), encoded a protein of 415 amino acid residues. The first 366 amino acid residues of snoN and snoA are the same, but subsequent amino acids show divergence. Several transcripts of h-ski (6.0, 4.7, 3.8, 3.0, 2.1 and 1.8 kb) were detected. The mRNAs of h-sno were 6.2, 4.4 and 3.2kb.
Molecular Oncology Laboratory, Nippon Veterinary and Zootechnical College, Tokyo, Japan.
N
N
Nomura
S
S
Sasamoto
S
S
Ishii
T
T
Date
M
M
Matsui
R
R
Ishizaki
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002460:Cell Line; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D004268:DNA-Binding Proteins; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D009857:Oncogenes; D011506:Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D012689:Sequence Homology, Nucleic Acid; D012984:Software
D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; C060615:SKIL protein, human; C060614:SKI protein, human; D004247:DNA