A modification of the thiobarbituric acid reaction.
A simple modification of the thiobarbituric acid reaction, which removes nonspecific chromogenic reaction products, involves incubation at room temperature for 16-20 hr and a column chromatographic step. The chromogen-concentrating capacity of the latter step is useful for determination of low concentrations of thiobarbituric acid-reactive materials in biological preparations.
UCLA/VA Medical Center, Sepulveda 91343.
T G
TG
Bidder
B M
BM
Sipka
D006801:Humans; D008314:Malonates / Q000032:analysis*; D008315:Malondialdehyde / Q000032:analysis* / Q000652:urine; D013053:Spectrophotometry; D013696:Temperature; D013858:Thiobarbiturates; D013997:Time Factors
D008314:Malonates; D013858:Thiobarbiturates; D008315:Malondialdehyde; C029684:thiobarbituric acid