Analysis of Aprt deficient mutants of Friend erythroleukaemia cells.
Cytogenetic analysis of mutants of the Friend Erythroleukaemia cell line deficient in adenine phosphoribosyl transferase (Aprt) was undertaken to ascertain whether non-disjunctional events were involved in the production of this mutation. All mutant clones examined were found to carry two copies of chromosome 8, the chromosome to which Aprt maps. Since the two homologues are distinguishable by silver staining, it was also clear that no mutants contained two copies of one homologue having lost the other. Treatment of mutants with 5-azacytidine failed to reactivate the Aprt locus.
O'Neill
R
R
Department of Biology, Queen's University of Belfast, N. Ireland.
Hickey
I
I
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000201:enzymology*
D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase