Effect of thermal injury in the rat on transfer of IgA protein into bile.
Severe thermal injury is associated with bacterial sepsis; the intestine is considered a likely source of invasive organisms. Because IgA antibody in bile accounts for much of the specific immune defense of the upper intestinal tract in the rat, the effect of thermal injury on the quantity of IgA protein in bile was examined. Sprague-Dawley rats received a 20% to 30% body surface area burn under anesthesia. Eighteen hours later the common bile duct was cannulated and bile was collected for three hours. Total IgA protein in bile decreased 90% after thermal injury. The bile volume, the concentration of bile protein, and free secretory component did not change significantly. Although blood flow to the liver 18 hours after thermal injury was not changed, there was a significant reduction in total IgA concentration in the circulation; both monomeric (m-IgA) and polymeric IgA (p-IgA) were decreased. This finding may explain, in part, the reduced concentration of IgA protein in bile. Although not examined in this study, decreased local hepatic synthesis and/or transport of p-IgA across the hepatocyte may also contribute to the reduced IgA levels in bile.
Department of Pediatrics, Harvard Medical School, Boston, Massachusetts.
P R
PR
Harmatz
E A
EA
Carter
D
D
Sullivan
R A
RA
Hatz
R
R
Baker
E
E
Breazeale
K
K
Grant
K J
KJ
Bloch
D000818:Animals; D001646:Bile / Q000276:immunology*; D001798:Blood Proteins / Q000032:analysis; D002056:Burns / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D007070:Immunoglobulin A / Q000378:metabolism*; D008102:Liver Circulation; D008970:Molecular Weight; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012635:Secretory Component / Q000032:analysis
D001798:Blood Proteins; D007070:Immunoglobulin A; D011506:Proteins; D012635:Secretory Component