Phosphorus-31 nuclear magnetic resonance study of the C6 glioma cell line cultured on microcarrier beads.
The energetic metabolism of perfused C6 glioma cells anchored and cultured on polystyrene microcarrier beads has been studied by phosphorus-31 nuclear magnetic spectroscopy (NMR). The observation of intracellular phosphorylated compounds demonstrates the metabolic long-lasting viability of the perfused cells. The effect of glucose deprivation on energetic metabolism and intracellular pH illustrates the existence of an active aerobic glycolysis. The non-invasive study of anchored C6 cells by NMR provides a direct means to investigate the metabolism of glioma cells.
Centre de Résonance Magnétique Biologique et Médicale, Faculté de Médecine, Marseille, France.
J
J
Vion-Dury
J P
JP
Galons
J
J
Fantini
S
S
Confort-Gouny
M
M
Kriat
P J
PJ
Cozzone
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism; D005910:Glioma / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008863:Microspheres; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D011137:Polystyrenes; D051381:Rats; D014407:Tumor Cells, Cultured
D010710:Phosphates; D011137:Polystyrenes; D010725:Phosphocreatine; D000255:Adenosine Triphosphate