The determination of anti-Toxoplasma gondii antibodies in different IgG subclasses of human sera by the enzyme-linked immunosorbent assay (ELISA).
The level of activity of the 4 IgG subclasses in toxoplasmosis were determined in an ELISA employing commercially available monoclonal antibodies to the 4 subclasses. Forty-four sera positive by IgG-ELISA and 73 negative sera were tested for IgG subclass activity. IgG1 was found to be the predominant subclass while IgG3 and IgG4 were probably produced at low but significant levels in sera which were positive for IgG antibodies. The significance of the results were discussed.
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Department of Microbiology, National University of Singapore.
Singh
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Yap
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D000328:Adult; D000818:Animals; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000097:blood* / Q000276:immunology
D007074:Immunoglobulin G