Tropism of canine neutrophils to xanthine oxidase.
Quantitative evaluation of neutrophil chemotaxis was performed on cells obtained by hypotonic-lysis techniques from heparinized blood samples from clinically normal dogs. The techniques resulted in neutrophil recovery rates between 60 and 80%. Chemotaxis comparisons were based on cellular migration in microchambers equipped with polycarbonate membranes with 5-micron pores. Chemo-attractant comparisons were based on neutrophil migration to medium, normal canine plasma, zymosan-activated plasma, and xanthine oxidase. Cellular migration to zymosan-activated plasma in buffer (1:100 dilution) was significantly (P less than 0.001) enhanced over random baseline medium migration. Neutrophil migrations to normal canine plasma and xanthine oxidase were quantitatively less than to zymosan-activated plasma, but were equivalent to each other and significantly greater than for random migration. Migration to xanthine oxidase was maximal at concentrations near 1 U/ml within 30 minutes.
Armed Forces Radiobiology Research Institute, Bethesda, MD 20814-5145.
D F
DF
Gruber
A M
AM
Farese
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004285:Dogs / Q000097:blood*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D014969:Xanthine Oxidase / Q000494:pharmacology*
D014969:Xanthine Oxidase