Energetic behaviour of mitochondria isolated from rat livers perfused with a perfluorodecalin + N,N-perfluorodiethylcyclohexylamine emulsion.
Rat livers have been perfused with a saline control medium or with a perfluorocarbon emulsion containing perfluorodecalin and N,N-perfluorodiethylcyclohexylamine, and the respiratory rates and transmembrane electrical potentials of mitochondria isolated following perfusion have been evaluated. The results indicate that the perfluorocarbon emulsion used, by providing a good oxygen supply to the perfused liver, allowed to preserve the efficiency of mitochondrial oxidative phosphorylation.
Department of Biological Chemistry, University of Padova, Italy.
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Branca
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Giron
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Conte
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Vincenti
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Scutari
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D004655:Emulsions; D004734:Energy Metabolism / Q000187:drug effects*; D005466:Fluorocarbons / Q000494:pharmacology*; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion; D051381:Rats
D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; C060999:N,N-perfluorodiethylcyclohexylamine; C008805:perfluorodecalin; D000255:Adenosine Triphosphate