Compared effect of n-3 and n-6 dietary fatty acids on rat intestinal acyl-CoA:cholesterol acyltransferase activity.
Polyunsaturated fatty acids are known to lower plasma cholesterol concentrations. We studied their effect on intestinal acyl-CoA:cholesterol acyltransferase (ACAT) activity in rats fed either salmon oil or corn oil (17% fat) with or without 1% cholesterol. After an 8-week feeding period we confirmed the hypolipidemic effect of salmon oil and we established its ability to stimulate ACAT activity in rats fed low-cholesterol diets. The most striking effect of 1% dietary cholesterol on ACAT activity was obtained in the control group (34% enhancement), whereas cholesterol supplementation had no effect on ACAT activity in the salmon oil group. The results enable us to suggest that n-3 fatty acids have an effect per se on ACAT activity; the regulation of enzyme activity by dietary cholesterol probably involves independent processes.
U 130 INSERM, Hôpital de Ste-Marguerite, Marseilles, France.
M
M
Chautan
E
E
Termine
N
N
Amirayan
J
J
Leonardi
A M
AM
Pauli
H
H
Portugal
H
H
Lafont
D000818:Animals; D002784:Cholesterol / Q000097:blood; D004032:Diet; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000201:enzymology*; D008099:Liver / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D002785:Sterol O-Acyltransferase / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol; D002785:Sterol O-Acyltransferase