Cooperative interaction of chicken lysozyme enhancer sub-domains partially overlapping with a steroid receptor binding site.
Expression of the lysozyme gene is a marker for the differentiation of macrophages, lysozyme transcription being gradually increased during maturation. We have analyzed the fine structure and function of two macrophage-specific enhancer elements of the chicken lysozyme gene (E-2.7 kb and E-0.2 kb). Both increase their activities upon LPS induction, both contain multiple binding sites for similar or identical nuclear factors and both can be divided into two functional modules. For the E-0.2 kb enhancer we found a synergistic activity of the modules to be dependent on their distance. Binding sites for nuclear proteins within enhancer E-0.2 kb overlap substantially with the previously identified progesterone/glucocorticoid receptor binding site, which is required for steroid induction of lysozyme transcription in the oviduct.
Genzentrum, Max-Planck-Institut für Biochemie, Martinsried, FRG.
J
J
Altschmied
M
M
Muller
A
A
Baniahmad
C
C
Steiner
R
R
Renkawitz
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005796:Genes; D008264:Macrophages / Q000378:metabolism; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics*; D009687:Nuclear Proteins / Q000378:metabolism; D010957:Plasmids; D011485:Protein Binding; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D015183:Restriction Mapping
D009687:Nuclear Proteins; D011965:Receptors, Glucocorticoid; D011980:Receptors, Progesterone; D004247:DNA; D009113:Muramidase