Factor, present in plasma, inhibiting the interaction of low density lipoprotein with arterial proteoglycan.
The complex formation of LDL with arterial proteoglycan or glycosaminoglycan was quantitated as precipitation of LDL-cholesterol after incubation in a low ionic strength buffer containing CaCl2 and MgCl2. It was found that human plasma or serum contains a factor which inhibits this complex formation. Upon density gradient centrifugation this factor was found at d greater than 1.24 g/ml. It could be further purified from the d greater than 1.24 g/ml fraction by affinity chromatography on heparin-Sepharose and hydrophobic interaction chromatography on phenyl-Sepharose. The content of inhibitory activity was found to vary over a 4-fold range in sera of apparently healthy persons.
Gaubius Institute TNO, Leiden, The Netherlands.
H J
HJ
Kempen
M
M
Buytenhek
E
E
Gruber
M
M
Bihari-Varga
D002122:Calcium Chloride / Q000494:pharmacology; D011232:Chemical Precipitation; D006025:Glycosaminoglycans / Q000097:blood* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000097:blood* / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D011509:Proteoglycans / Q000097:blood* / Q000378:metabolism
D006025:Glycosaminoglycans; D008077:Lipoproteins, LDL; D011509:Proteoglycans; D015636:Magnesium Chloride; D008274:Magnesium; D002122:Calcium Chloride