Synthesis and biological properties of 4-norleucine-neuropeptide Y; secondary structure of neuropeptide Y.
Neuropeptide Y (NPY) is a 36 amino acid peptide amide isolated from porcine brain. The NPY analog, 4-norleucine-NPY was synthesized by a solid-phase method and purified to homogeneity in 20% yield by reverse-phase chromatography. Investigation of the biological properties indicated that the analog is an agonist of NPY. Secondary structural analyses revealed that NPY and the analog exhibited predominantly alpha-helical and beta-sheet structures, respectively; however, experiments in trifluoroethanol indicated that the analog has the potential of assuming an alpha-helical structure. Based on circular dichroism (CD), Raman spectroscopy and Chou-Fasman analyses, a model has been proposed for the secondary structure of NPY.
Department of Surgery, University of Cincinnati Medical Center, OH 45267.
A
A
Balasubramaniam
V
V
Renugopalakrishnan
D F
DF
Rigel
M S
MS
Nussbaum
R S
RS
Rapaka
J C
JC
Dobbs
L A
LA
Carreira
J E
JE
Fischer
D000595:Amino Acid Sequence; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002942:Circular Dichroism; D005583:Fourier Analysis; D006339:Heart Rate / Q000187:drug effects; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D011487:Protein Conformation; D051381:Rats; D013059:Spectrum Analysis, Raman; D014661:Vasoconstriction / Q000187:drug effects
D009478:Neuropeptide Y; C054642:neuropeptide Y, Nle(4)-