Assessment of membrane protection by 31P-NMR effects of lidocaine on calcium-paradox in myocardium.
In studying calcium paradox, perfused rat hearts were used to investigate the myocardial protective effects of lidocaine. Intracellular contents of phosphates were measured using the 31P-NMR method. In hearts reexposed to calcium, following 3 minute calcium-free perfusion, a rapid contracture occurred, followed by rapid and complete disappearance of intracellular phosphates with no resumption of cardiac function. In hearts where lidocaine was administered from the onset of the calcium-free perfusion until 2 minutes following the onset of reexposure to calcium, both intracellular phosphates and cardiac contractility were maintained. Therefore, it can be said that cell membranes were protected by lidocaine.
First Department of Internal Medicine, Osaka City University Medical School, Japan.
H
H
Sakai
M
M
Yoshiyama
M
M
Teragaki
K
K
Takeuchi
T
T
Takeda
M
M
Ikata
M
M
Ishikawa
I
I
Miura
D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D004734:Energy Metabolism / Q000187:drug effects; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008012:Lidocaine / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D010758:Phosphorus; D051381:Rats; D011921:Rats, Inbred WKY
D010758:Phosphorus; D008012:Lidocaine; D002118:Calcium