An effective method for plasma lipoprotein separation: studies of various animal species.
1. Plasma lipoprotein separation by density gradient ultracentrifugation largely depends on visual examination based on the natural yellow pigments of lipoproteins. 2. In non-human species and in humans with dyslipoproteinemia, some lipoproteins are not well visualized due to the lack of pigments. 3. Using a fluorescent probe in minute quantity (1,6 diphenyl-1,3,5-hexatriene) we were able to demonstrate an effective plasma lipoprotein separation using a discontinuous density gradient ultracentrifugation technique. 4. Plasma lipoproteins of human, chicken, rat and carp were compared showing the unique character of carp HDL.
Lipid Research Unit, Rambam Medical Center, Technion Haifa, Israel.
M
M
Aviram
S
S
Mokady
U
U
Cogan
D000818:Animals; D002347:Carps; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002784:Cholesterol / Q000097:blood; D004161:Diphenylhexatriene; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D051381:Rats; D013045:Species Specificity; D014280:Triglycerides / Q000097:blood
D005456:Fluorescent Dyes; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D004161:Diphenylhexatriene; D002784:Cholesterol