The effect of fluorocarbon emulsion on 24-hour canine heart preservation by coronary perfusion.
This study was designed to evaluate the effects of fluorocarbon emulsion as an oxygen carrier in myocardial preservation. The hearts were preserved for 24 hours by coronary perfusion with either oxygenated crystalloid cardioplegic solution (group C) or crystalloid cardioplegic solution with fluorocarbon added (group FC). The perfusion pressure was kept at 20 mm Hg, and myocardial temperature was maintained at 4 degrees C. Group FC demonstrated better recovery in developed pressure and maximum rate of rise of left ventricular pressure compared with group C. The postpreservation end-diastolic pressure in group C increased significantly compared with the baseline value (value obtained before preservation). On the other hand, group FC showed no significant increase of end-diastolic pressure after preservation. Group FC released a significantly lower level of creatine kinase into its perfusate than did group C. Ultrastructural changes after preservation in group C showed severe ischemic injury, but there was no evidence of ischemic injury in group FC. The use of fluorocarbon emulsion proved beneficial to myocardial protection in long-term preservation of canine hearts.
First Department of Surgery, Yamaguchi University School of Medicine, Ube, Japan.
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Gohra
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Mori
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Esato
D000818:Animals; D001802:Blood Substitutes; D002314:Cardioplegic Solutions; D004285:Dogs; D004655:Emulsions; D005466:Fluorocarbons; D006321:Heart; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000648:ultrastructure; D009926:Organ Preservation; D013997:Time Factors
D001802:Blood Substitutes; D002314:Cardioplegic Solutions; D004655:Emulsions; D005466:Fluorocarbons; C008807:perfluorotributylamine