Biotinylation: a simple method for labelling complement component C8 with preservation of functional activity.
Biotinylation of human C8 with the water-soluble biotin derivative biotinylamidohexanoic acid, N-hydroxysulfosuccinimide ester is an excellent method for labelling this terminal complement component with preservation of its functional activity. The biotinylated product can be detected both in native form and also following its incorporation into the terminal complement complexes. Detection assays include Western blotting, crossed immunoblotting, ELISA, and immunocytochemistry. Biotinylation is an attractive alternative method for labelling C8 and may be used for detecting and quantifying C8 and C5b-9 complexes in their soluble and membrane-bound forms.
Institute of Medical Microbiology, University of Giessen, F.R.G.
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S
Bhakdi
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Roth
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F
Hugo
D001710:Biotin; D003185:Complement C8 / Q000032:analysis* / Q000502:physiology; D015938:Complement Membrane Attack Complex; D003165:Complement System Proteins / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans
D003185:Complement C8; D015938:Complement Membrane Attack Complex; D001710:Biotin; D003165:Complement System Proteins