Growth hormone-induced alteration of morphology and tubulin expression in 3T3 preadipose cells.
Effects of growth hormone on morphology and cytoskeletal protein expression were examined in 3T3-F442A preadipocytes in serum-free medium. Between 2 and 5 days of culture 2 nM methionyl human growth hormone converted 3T3-F442A cells from a flat fibroblastic morphology to a rounded form with numerous membrane convolutions. Growth hormone treated cultures manifested a 30-40% reduction in cell volume. Growth hormone induced changes in morphology and volume preceded and were independent of lipogenesis. In cells treated with growth hormone, expression of alpha and beta-tubulin as determined by Western blotting was found to increase approximately 50% within 72 h as compared to untreated cells. After 7 days, tubulin levels in growth hormone treated cells were approximately 40% of control levels. This indicated that morphological changes and alteration of tubulin expression were signatures of growth hormone action on 3T3-F442A cells.
Memorial Sloan-Kettering Cancer Center, New York, N.Y. 10021.
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Guller
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Corin
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Wu
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Sonenberg
D000273:Adipose Tissue / Q000378:metabolism* / Q000648:ultrastructure; D015153:Blotting, Western; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D014404:Tubulin / Q000235:genetics*
D003598:Cytoskeletal Proteins; D014404:Tubulin; D013006:Growth Hormone