Highly sensitive assays of autoantibodies to thyroglobulin and to thyroid peroxidase.
These highly sensitive assays are based on the interaction between thyroid autoantibodies and 125I-labeled autoantigens. Serum samples are incubated with labeled thyroid peroxidase (TPO) or thyroglobulin (Tg) to allow the formation of antibody-labeled antigen complexes. The complexes are then precipitated by addition of solid-phase Protein A. In the presence of high concentrations of TPO antibody or Tg antibody, more than 50% of the respective labeled antigen was precipitated, whereas only 1-2% was precipitated in the absence of autoantibody. Interassay CVs were 3.2% and 5.7%, respectively, for the anti-TPO and anti-Tg assays. There was no cross-reactivity between Tg antibody and TPO antibody. Results correlated highly significantly with results from other assay systems based on antigen-coated cells or plastic supports, but the assays described here were considerably more sensitive. Scatchard analysis of the assay data provided information on the affinity and serum concentration of TPO autoantibodies (ka approximately 10(9) L/mol and concentrations up to 1 g/L) and Tg autoantibodies (ka approximately 4 x 10(10) L/mol and concentrations up to 1 g/L). Overall, these assays provide a sensitive, precise, and convenient system for measuring and investigating the properties of thyroid autoantibodies.
RSR Ltd., Pentwyn, Cardiff, U.K.
K
K
Beever
J
J
Bradbury
D
D
Phillips
S M
SM
McLachlan
C
C
Pegg
A
A
Goral
W
W
Overbeck
G
G
Feifel
B R
BR
Smith
D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000032:analysis*; D006801:Humans; D007453:Iodide Peroxidase / Q000032:analysis / Q000276:immunology*; D011863:Radioimmunoassay; D013696:Temperature; D013954:Thyroglobulin / Q000032:analysis / Q000276:immunology*; D013997:Time Factors
D001323:Autoantibodies; D013954:Thyroglobulin; D007453:Iodide Peroxidase