Continuous-flow 13C-filtered 1H NMR spectroscopy of ethanol metabolism in rat liver perfusate.
Using a 188.5-microliters continuous-flow dual probe 1H[13C] spin-echo difference spectra of rat liver perfusate were acquired. The conversion of [1-13C]ethanol to [1-13C]-acetaldehyde was readily monitored as a function of time. In combination with 1-1 water nonexcitation and WALTZ 13C decoupling, this method proved to be superior in sensitivity and selectivity to direct 1H or 13C detection.
Albert
K
K
Institut für Organische Chemie der Universität Tübingen, Federal Republic of Germany.
Sudmeier
J L
JL
Anwer
M S
MS
Bachovchin
W W
WW
D000818:Animals; D002247:Carbon Isotopes; D004867:Equipment Design; D000431:Ethanol / Q000378:metabolism* / Q000493:pharmacokinetics; D006859:Hydrogen; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D002247:Carbon Isotopes; D000431:Ethanol; D006859:Hydrogen