Simultaneous measurement of cytosolic free calcium concentration and cell circumference during contraction, both in a single rat cardiomuscular cell, by digital imaging microscopy with indo-1.
We have developed a novel system using digital imaging microscopy with indo-1 to measure the cytosolic free calcium concentration [( Ca2+]i). The method is particularly suitable for measuring the rapid change in [Ca2+]i in relation to the cell motion. With this system, we made the first successful simultaneous measurement of [Ca2+]i and cell circumference during contraction in an electrically stimulated single rat ventricular myocyte. It was found that the level of [Ca2+]i was elevated during contraction, and that the onset and peak time of the calcium transient preceded that of the decrease in circumference.
Tamura
K
K
Research Laboratories, Chugai Pharmaceutical Co., Ltd., Tokyo, Japan.
Yoshida
S
S
Fujiwake
H
H
Watanabe
I
I
Sugawara
Y
Y
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002451:Cell Compartmentation; D003593:Cytoplasm / Q000502:physiology; D004558:Electric Stimulation; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007211:Indoles; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology*; D051381:Rats
D005456:Fluorescent Dyes; D007211:Indoles; C048960:indo-1; D002118:Calcium