Recombinant and native human urinary colony-stimulating factor directly augments granulocytic and granulocyte-macrophage colony-stimulating factor production of human peripheral blood monocytes.
Colony-stimulating factor from human urine (CSF-HU) has been purified to a homogeneous protein, and its complementary DNA (cDNA) has been cloned. Recombinant CSF-HU was prepared from a serum-free medium conditioned by Chinese hamster ovary cells transfected with the cDNA and purified by the same method as that for the native protein. Purified CSF-HU stimulated human bone marrow cells to form macrophage colonies. It also stimulated human mature monocytes prepared from peripheral blood of healthy volunteers to produce human active colony-stimulating activity that stimulates human bone marrow cells to form granulocyte and macrophage colonies. This activity was partially neutralized by the addition of both polyclonal antibodies against human granulocyte colony-stimulating factor and against human granulocyte-macrophage CSF, respectively. The stimulation of monocytes by CSF-HU was not inhibited by the addition of polymyxin-B, which is known as a potent inhibitor of endotoxin. On the other hand, CSF-HU did not stimulate monocyte production of interleukin-1 and interferon. These results indicate that recombinant and native CSF-HU stimulates immature cells as well as mature cells in the human monocyte lineage.
Division of Hemopoiesis, Jichi Medical School, Tochigi, Japan.
K
K
Motoyoshi
K
K
Yoshida
K
K
Hatake
M
M
Saito
Y
Y
Miura
N
N
Yanai
M
M
Yamada
T
T
Kawashima
G G
GG
Wong
P A
PA
Temple
D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D004247:DNA / Q000235:genetics; D006098:Granulocytes; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008264:Macrophages; D011112:Polymyxin B / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D014556:Urine / Q000032:analysis*
D003115:Colony-Stimulating Factors; D007370:Interferon Type I; D007375:Interleukin-1; D011994:Recombinant Proteins; D004247:DNA; D011112:Polymyxin B