[Mechanism of action of interferon is linked to its ability to protect the cell from interstrand cross-linking of DNA, induced by 8-methoxypsoralen, activated by long-wave UV-light].
Treatment of human lymphocytes in the G1 phase of mitotic cycle with human lymphoblastoid interferon (Ly-IFN) decreased the frequencies of chromosome aberrations induced by 8-methoxy-psoralen-induced interstrand cross-links. Anticlastogenic effect of Ly-IFN was accompanied by stimulation of unscheduled synthesis of DNA in the G2 phase of mitotic cycle, as shown by increased percent of labeled cells registered by 3H-thymidine autoradiography. The data obtained seem to indicate that the mechanism of Ly-IFN protection is connected with stimulation of postreplicative repair.
G N
GN
Makedonov
E L
EL
Iakubovskaia
G D
GD
Zasukhina
D002869:Chromosome Aberrations / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007399:Interphase / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008730:Methoxsalen / Q000633:toxicity*; D009154:Mutation; D014466:Ultraviolet Rays
D007370:Interferon Type I; D004247:DNA; D008730:Methoxsalen
Mekhanizm deĭstviia interferona sviazan s ego sposobnost'iu kletku ot mezhnitevykh sshivok DNK, indutsirovannykh 8-metoksipsoralenom, aktivirovannym dillinovolnovym UF-svetom.