Effect of gamma irradiation on the human immunodeficiency virus and human coagulation proteins.
The effect of gamma irradiation on HIV and plasma coagulation factors F VIII:C, F VIII:vWF and FIX was studied. Donor plasma was harvested from single donations, frozen and irradiated in the frozen state at target doses from 0 to 40 kGy (0-4 mRad). HIV was inoculated into human plasma and irradiated in a similar manner. A range of other viruses, not suspended in plasma, were also irradiated to establish viral inactivation. An inactivation rate of 0.164 TCID50 dose/ml/kGy was demonstrated for HIV compared to rates of 0.00173, 0.00526 and 0.00286 log10 units/ml/kGy for F VIII:C,F VIII:vWF and FIX respectively. The use of gamma irradiation to inactivate infectious agents present in human plasma may eliminate the need for any post-production viral inactivation methods and provide a means of assuring the safety of as yet untreated products such as cryoprecipitate and fresh frozen plasma.
Kitchen
A D
AD
Regional Blood Transfusion Centre, Brentwood, Essex, UK.
Mann
G F
GF
Harrison
J F
JF
Zuckerman
A J
AJ
D001779:Blood Coagulation Factors / Q000528:radiation effects*; D001780:Blood Coagulation Tests; D005720:Gamma Rays; D006678:HIV / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques
D001779:Blood Coagulation Factors