Possible role of a 36 kDa protein induced by respiratory inhibitors in cyanide-resistant respiration in Hansenula anomala.
The antimycin A or cyanide-dependent appearance of a 36 kDa protein in the particulate fraction was observed in L-[35S]methionine pulse-labeling experiments on cells of Hansenula anomala, in which cyanide-resistant respiration was induced. The combined addition of cycloheximide or anaerobiosis, which block the induction of cyanide-resistant respiration, repressed the synthesis of this protein. These results suggest the involvement of the particulate 36 kDa protein in cyanide-resistant respiration.
Department of Biochemistry, Niigata College of Pharmacy.
A
A
Yoshimoto
S
S
Sakajo
N
N
Minagawa
T
T
Komiyama
D000963:Antimetabolites / Q000494:pharmacology*; D001203:Ascomycota / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis*; D010101:Oxygen Consumption / Q000187:drug effects; D013347:Subcellular Fractions / Q000378:metabolism; D013462:Sulfur Radioisotopes
D000963:Antimetabolites; D003486:Cyanides; D005656:Fungal Proteins; D013462:Sulfur Radioisotopes; D003513:Cycloheximide