Isolation and characterization of a mutant of Haemophilus influenzae type b deficient in outer membrane protein P1.
The gene for outer membrane protein P1 of Haemophilus influenzae type b has been previously cloned and expressed in Escherichia coli. To investigate the physiologic role of the P1 protein, the cloned P1 gene was insertionally inactivated with the Tn5 derivative Tn5tac1, and an isogenic P1-deficient Haemophilus mutant was then generated by transformation with linearized plasmid DNA containing the insertionally inactivated gene. The P1-deficient strain grew normally in vitro and induced bacteremia in the infant rat model.
Munson
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Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.
Hunt
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D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006193:Haemophilus influenzae / Q000235:genetics*; D008970:Molecular Weight; D009154:Mutation
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial