[Identification of the segment for binding of transferrin using a cellular receptor].
Methods of proteolysis, radio-immunoblotting and affinity chromatography were used for identifying the human transferrin molecular binding site with cellular receptor. Monoclonal antibody HTF-14 which inhibits binding of the transferrin molecule with the receptor was employed. We showed that this monoclonal antibody has an antigenic determinant of the conformational type which is localized on the COOH-sublobe of the NH2-lobe of the molecule of the transferrin.
Rubikaĭte
B I
BI
Viklitskiĭ
V
V
Slavichkova
A
A
Dushkova
B
B
Beresten'
S F
SF
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002846:Chromatography, Affinity; D002918:Chymotrypsin; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D011487:Protein Conformation; D011990:Receptors, Transferrin / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism*; D014357:Trypsin
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D011990:Receptors, Transferrin; D014168:Transferrin; D002918:Chymotrypsin; D014357:Trypsin
Identifikatsiia uchastka sviazyvaniia transferrina s kletochnym retseptorom.