The influence of temperature and serum deprivation on the synthesis of heat-shock proteins and alpha and beta tubulin in promastigotes of Leishmania major.
We have examined changes in the relative synthesis of individual proteins in promastigotes of Leishmania major subjected to decreasing serum levels in vitro. We observed increases in the relative synthesis of the putative heat-shock proteins of 82 and 70 kDa and of proteins of 79 and 41 kDa but decreases in the synthesis of proteins of 38 and 28 kDa. The relative synthesis of alpha-tubulin increased, whereas that of beta-tubulin decreased, in promastigotes subjected to decreased serum concentrations. This uncoordinated regulation of the synthesis of the tubulin proteins was not reflected as an alteration in the relative levels of the messenger RNA of the respective proteins. We have also studied changes in the synthesis of proteins in L. major promastigotes subjected to a temperature change from 26 degrees C to 34 degrees C. The results indicate that the synthesis of putative heat-shock proteins of 82, 70, 65, 41, 23 and 22 kDa increased when the parasites were incubated at the higher temperature, although these proteins were synthesised in detectable amounts at 26 degrees C. We could not detect differences between infective and non-infective promastigotes, separated by binding to peanut agglutinin, in the synthesis of individual proteins in response to increased temperature. These results were confirmed by densitometer analysis of autoradiographs of labelled promastigote proteins, and the relative changes in the synthesis of the two major heat-shock proteins, as well as alpha- and beta-tubulin, were estimated.
Toye
P
P
Department of Medicine, Harvard Medical School, Boston, MA.
Remold
H
H
D000818:Animals; D001345:Autoradiography; D001769:Blood; D015152:Blotting, Northern; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology; D007895:Leishmania tropica / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D008970:Molecular Weight; D019887:Peanut Agglutinin; D012333:RNA, Messenger / Q000096:biosynthesis; D013696:Temperature; D014404:Tubulin / Q000096:biosynthesis* / Q000235:genetics
D006360:Heat-Shock Proteins; D037102:Lectins; D019887:Peanut Agglutinin; D012333:RNA, Messenger; D014404:Tubulin