Immunocytochemical detection of proliferating cells in the rat tooth germ by monoclonal antibodies against 5-bromo-2'-deoxyuridine.
5-Bromo-2'-deoxyuridine incorporated into DNA synthesizing cells in vivo is detected by a monoclonal antibody. The results are similar to those obtained with cytoautoradiography, but show variance with data from some past autoradiographic studies. This immunocytochemical technique may be a useful tool for study of cell kinetics in the oral tissues.
Istituto di Istologia ed Embriologia gen., University of Pavia, Italy.
A
A
Casasco
A
A
Calligaro
C
C
Marchetti
P
P
Poggi
S
S
Brugnatelli
M
M
Danova
R
R
Fiocca
D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine; D002455:Cell Division; D007150:Immunohistochemistry; D051381:Rats; D014083:Tooth Germ / Q000166:cytology*
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine