RNA polymerase II transcription complexes are destabilized by ATP or GTP.
In vitro transcription by RNA polymerase II requires hydrolysis of the beta-gamma bond of ATP after the transcription complex forms, prior to RNA synthesis. It was observed that the presence of ATP during transcription complex formation inhibits subsequent transcription when the remaining 3 rNTPs are added. We now report that ATP or GTP inhibits transcription if either is present during transcription complex formation to added to preformed complexes. This inhibition is not due to purine rNTP degradation and occurs if as little as 2 mM ATP or 50 mM GTP is added to forming or preformed complexes. Deoxy derivatives of ATP inhibit similarly. AMP-PNP, a beta-gamma imido derivative, neither satisfies the energy requirement nor inhibits transcription if added to incubations of forming or of preformed transcription complexes.
Kundzicz
H
H
Biology Department, University of Massachusetts, Boston 02125.
Davis
E A
EA
Ackerman
S
S
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002467:Cell Nucleus / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D006367:HeLa Cells; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D012319:RNA Polymerase II / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013997:Time Factors; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D012319:RNA Polymerase II