Furazolidone-induced injury in the isolated perfused chicken heart.
The Langendorff isolated heart preparation was adapted to determine the effect of furazolidone (0.5 and 2 micrograms/ml of perfusate) on hearts of 3-week-old broiler chickens. Following 115 minutes of perfusion, both concentrations of furazolidone caused approximately a two-fold increase in myocardial vascular resistance and a six-fold increase in lactate dehydrogenase release into the effluent fluid, compared with a control perfused group of isolated hearts (P less than 0.01). Ultrastructural alteration differences were not found between the drug-treated and control groups. It was concluded that: (i) furazolidone, at concentrations only moderately above therapeutic plasma concentrations, caused detrimental changes in myocardial vascular resistance and lactate dehydrogenase release and (ii) the isolated chicken heart preparation is an example of a cost-effective, reliable laboratory tool for screening potential cardiotoxins.
McCallum
T
T
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayetee, IN 47907.
Badylak
S F
SF
Van Vleet
J F
JF
Reed
W M
WM
D000818:Animals; D002645:Chickens / Q000502:physiology*; D005664:Furazolidone / Q000633:toxicity*; D006321:Heart / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology / Q000648:ultrastructure; D010477:Perfusion / Q000662:veterinary; D014655:Vascular Resistance / Q000187:drug effects*
D005664:Furazolidone; D007770:L-Lactate Dehydrogenase