Labelling monoclonal antibodies with yttrium-90.
Using the cyclic DTPA derivatisation procedure developed by Hnatowich, conditions have been optimised for labelling three tumour-associated monoclonal antibodies with 90Y. High labelling efficiencies (greater than 95%) at modest specific activities (1 mCi/mg) could be routinely obtained. Radiochemical stability of the radiolabelled preparations in vitro was good, but radiolysis resulted in early losses of antibody immunoreactivity.
Mather
S J
SJ
Department of Nuclear Medicine, St. Bartholomews Hospital, London, UK.
Tolley
D M
DM
White
G W
GW
D000911:Antibodies, Monoclonal; D004355:Drug Stability; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D004369:Pentetic Acid; D015021:Yttrium Radioisotopes
D000911:Antibodies, Monoclonal; D015021:Yttrium Radioisotopes; D004369:Pentetic Acid