Molecular characterization of a surface protein antigen gene from serotype c Streptococcus mutans, implicated in dental caries.
The complete nucleotide sequence of the gene for a cell-surface protein antigen (PAc) of Streptococcus mutans MT8148 (serotype c) was determined. The pac gene consisted of 4695 bp and coded for a 170773D protein. The pac gene product contained a putative 38 amino acid signal peptide, resulting in a 166817D mature protein. A potential promoter sequence and a putative Shine-Dalgarno sequence preceded the open reading frame. Two internal repeating amino acid sequences were present in the PAc. One repeating region located in the N-terminal region was rich in alanine, and the other located in the central region was rich in proline. Southern blot analysis under the less stringent condition (allowing up to 35% base mismatch) revealed that the probe covering the proline-rich region hybridized to DNA preparations from strains of Streptococcus cricetus, Streptococcus sobrinus and Streptococcus downei as well as Streptococcus mutans.
Department of Dental Research, National Institute of Health, Tokyo, Japan.
N
N
Okahashi
C
C
Sasakawa
M
M
Yoshikawa
S
S
Hamada
T
T
Koga
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015139:Blotting, Southern; D003062:Codon; D004269:DNA, Bacterial / Q000235:genetics*; D003731:Dental Caries / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000235:genetics; D015183:Restriction Mapping; D012703:Serotyping; D013295:Streptococcus mutans / Q000145:classification / Q000235:genetics* / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D003062:Codon; D004269:DNA, Bacterial; D008565:Membrane Proteins; D021382:Protein Sorting Signals