Long-term storage of Campylobacter pylori.
A reproducible method for long-term storage of Campylobacter pylori has been previously described. We cultured 10 strains of C. pylori in brucella broth that was supplemented with 10% fetal bovine serum. After incubation for 24 h, 10% glycerol and an additional 10% fetal bovine serum were added to the cultures, which when then held in storage at -70 degrees C. After 6 months, each of the strains remained viable. This technique, therefore, represents an excellent method for long-term storage of C. pylori isolates.
Department of Pediatrics, Hospital for Sick Children, University of Toronto, Ontario, Canada.
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B
Drumm
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Sherman
D002167:Campylobacter / Q000254:growth & development*; D003470:Culture Media; D005615:Freezing; D006801:Humans; D011309:Preservation, Biological
D003470:Culture Media