Propranolol-induced changes in the catecholamine-containing cells and vesicles of SHR sympathetic ganglia.
The ultrastructural effects of long-term propranolol administration on the catecholamine-containing (CC) cells of cervical sympathetic ganglia were studied in spontaneously hypertensive rats. After 2 months of propranolol administration, there were hydrops of mitochondria, together with swelling and vesiculation of Golgi complex and rough endoplasmic reticulum. Such swollen organelles seemed to coalesce with each other, resulting in transformation of major parts of the cytoplasm into large membrane bound saccules. In that group of animals, the number of CC vesicles showed a significant decrease (P less than 0.025) compared to the control. Moreover, such vesicles looked more electron dense, with decreased electron-lucent haloes compared to the control. Blood pressure recording via rat tail cuff showed a significant drop (P less than 0.0001) of the mean systolic blood pressure in the treated animals. One month after stopping propranolol administration, resorption of the cytoplasmic saccules occurred, but the cytoplasm looked lighter and lost its granular appearance. The mitochondria regained their normal shape, though they appeared fewer in number. The CC vesicles were significantly reduced in number (P less than 0.001) and were less electron dense compared to the control. The possible implications of these findings on the mechanism of action of propranolol as an antihypertensive agent are discussed.
Division of Anesthesiology, Cleveland Clinic Foundation, Ohio 44195.
N A
NA
Mekhail
F G
FG
Estafanous
P A
PA
Khairallah
B
B
Healy
D000818:Animals; D001794:Blood Pressure; D002395:Catecholamines / Q000378:metabolism*; D003593:Cytoplasm / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D011433:Propranolol / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR / Q000033:anatomy & histology / Q000502:physiology*; D011919:Rats, Inbred Strains / Q000502:physiology*; D013997:Time Factors
D002395:Catecholamines; D011433:Propranolol