[Interference of inflammation caused by polyester with the growth of Yoshida ascite hepatoma. IV. Modifications of the sedimentation profile of the hepatoma cells].
It was previously shown that Yoshida's ascites-hepatoma cells (Y cells) intraperitoneally injected into rats carrying chronic inflammation caused by polyester thread rapidly disappear. In this paper the sedimentation rate in a continuous Percoll gradient at unit gravity (1 g) using the CELSEP apparatus (Sorvall), the mean diameter and volume of the Y cells harvested from the peritoneal cavity of untreated (NT) and polyester-treated (TM) animals were determined at 6-12-18-24 hours after the inoculum. At 6 hours a number of Y cells appeared enlarged both from TM and NT rats, while afterwards, and notably at 18 and 24 hours, their size was decreased and their sedimentation rate was reduced only in TM animals. It seems that the chronic inflammation causes alterations both in the size and in the density of the Y cells and probably one of the mechanisms of their death is through shrinkage and apoptosis.
Torboli
M
M
Melandri
P
P
Ventrelli
I
I
Berti
G
G
Benassi
G
G
D000818:Animals; D001201:Ascites / Q000473:pathology*; D007249:Inflammation / Q000209:etiology / Q000503:physiopathology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D011091:Polyesters; D051381:Rats; D011919:Rats, Inbred Strains
D011091:Polyesters
Interferenza dell 'infiammazione da poliestere con la crescita dell'epatoma ascite di Yoshida. IV. Modificazioni del profilo di sedimentazione delle cellule dell'epatoma.