Thrombospondin and fibronectin are synthesized by neutrophils in human inflammatory joint disease and in a rabbit model of in vivo neutrophil activation.
Using 35S-methionine metabolic labeling, we studied de novo synthesis and secretion of proteins by activated polymorphonuclear neutrophils (PMN) from two different sources. PMN isolated from inflammatory synovial fluid of patients with inflammatory joint disease were first analyzed. The protein synthetic activity of these cells was compared with that of nonactivated PMN isolated from the peripheral blood of the same patient. Similar studies were conducted on glycogen-activated PMN from the peritoneal cavity of rabbits and results were compared with nonactivated peripheral blood PMN isolated from the same rabbit. Cells were labeled for a period of 16 to 20 h and supernatants were analyzed by one and two dimensional gel electrophoresis. In both models, the activated PMN showed a marked increase in the synthesis and secretion of thrombospondin as identified by immunoisolation with antibodies to this protein. The production of thrombospondin by activated cells paralleled a similar increase in production of another extracellular matrix and cell adhesion protein, fibronectin. The proportion of thrombospondin synthesis and secretion relative to total protein was approximately 1% in both human- and rabbit-activated PMN. For fibronectin, this proportion was in the 0.02% range. Although fibronectin mRNA accumulation in activated PMN could be demonstrated by Northern blots, we were not able to obtain similar results for thrombospondin mRNA. This could be caused by the rapid turnover of this transcript because it is known to contain an adenine uridine-rich 3' untranslated sequence. We conclude that activated PMN are capable of producing thrombospondin. Furthermore, glycogen-activated rabbit peritoneal fluid PMN represent a valuable and relevant source of activated PMN for studying the protein synthetic events of these cells in the context of inflammation.
Kreis
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C
Unité de recherche Inflammation et Immunologie-Rhumatologie, Université Laval, Quebec City, Canada.
La Fleur
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M
Ménard
C
C
Paquin
R
R
Beaulieu
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AD
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D003470:Culture Media / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005353:Fibronectins / Q000096:biosynthesis*; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D019699:Thrombospondins
D003470:Culture Media; D005353:Fibronectins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019699:Thrombospondins