Interleukin 1 plus interleukin 3 plus colony-stimulating factor 1 are essential for clonal proliferation of primitive myeloid bone marrow cells.
The clonal growth in nutrient agar at low cell densities of high-proliferative potential colony-forming cells (HPP-CFC) of bone marrow obtained from mice treated 2 days earlier with 5-fluorouracil (FU) (FU2dBM) has been shown to require a combination of three growth factors, interleukin 1 (IL-1), interleukin 3 (IL-3), and macrophage colony-stimulating factor (CSF-1). These HPP-CFC have been enriched 140-fold from FU2dBM by fluorescence-activated cell sorting of 7/4-, B220-, and L3T4-negative cells. The mean of the plating efficiencies of these enriched populations was 4.4% and no growth was observed when the factors were used singly. Similarly, enrichments of 16-fold were obtained from FU2dBM using immunomagnetic Dynabeads with anti-7/4 plus anti-B220 (meaning plating efficiency 0.5%). The further additions of human granulocyte CSF or mouse granulocyte-macrophage CSF or both to IL-1 plus IL-3 plus CSF-1 did not increase HPP-CFC colony formation, but both augmented the small colony formation with IL-1 plus IL-3, IL-3 plus CSF-1, or IL-1 plus CSF-1.
Cell Biology Group, Peter MacCallum Cancer Institute, Melbourne, Victoria, Australia.
S H
SH
Bartelmez
T R
TR
Bradley
I
I
Bertoncello
D Y
DY
Mochizuki
R J
RJ
Tushinski
E R
ER
Stanley
A J
AJ
Hapel
I G
IG
Young
A B
AB
Kriegler
G S
GS
Hodgson
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D002999:Clone Cells / Q000187:drug effects / Q000502:physiology; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D004338:Drug Combinations; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000502:physiology; D007375:Interleukin-1 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D008297:Male; D051379:Mice
D003115:Colony-Stimulating Factors; D004338:Drug Combinations; D007375:Interleukin-1; D007377:Interleukin-3