Rapid and sensitive determination of beta-phenylethylamine in animal brains by high performance liquid chromatography with fluorometric detection.
A reversed phase HPLC method with fluorometric detection for the analysis of beta-phenylethylamine has been developed using p-methoxyphenylethylamine as an internal standard. Two columns, containing 200 microL of Dowex 50-X8 and Amberlite CG-50 respectively, were used to prepare a fraction containing beta-phenylethylamine. The recoveries of beta-phenylethylamine and p-methoxyphenylethylamine were 53.9 +/- 9.4% and 68.1 +/- 12.4%, respectively, and elution profile of p-methoxyphenylethylamine was sufficiently well correlated with that of beta-phenylethylamine. Regional distributions of beta-phenylethylamine in rat and mouse brains were determined. The highest concentrations were found in hypothalamus and hippocampus in both animals.
Department of Neuropsychiatry, Kyoto Prefectural University of Medicine, Japan.
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Taga
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Tsuji
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Nakajima
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008832:Microchemistry; D009928:Organ Specificity; D010627:Phenethylamines / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D010627:Phenethylamines; C029261:phenethylamine