Effects of gamma-ray irradiation in utero on developing hearts of rat embryos: inhibition of accumulation of a 27-kDa protein.
Rat embryos were irradiated in utero with 1.5-Gy (teratogenic dose) of 60Co gamma-rays on Day 9 of gestation, and the protein composition of each embryonic heart was examined by two-dimensional electrophoresis on Day 18. Almost all of about 450 protein species detected were identical to those of the non-irradiated hearts. However, a 27-kDa protein was reproducibly found in decreased amount in the irradiated samples. In the normal development of the heart, this protein accumulates rapidly either during the last several days of gestation or upon birth. Therefore, the irradiation with gamma-rays inhibited the accumulation of this protein.
Higo
H
H
Department of Geneticopathology, Hiroshima University, Japan.
Higo
K
K
Lee
J Y
JY
Satow
Y
Y
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005318:Fetal Heart / Q000528:radiation effects*; D005720:Gamma Rays; D005865:Gestational Age; D009124:Muscle Proteins / Q000096:biosynthesis*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats
D009124:Muscle Proteins