Hb Moriguchi or alpha 2 beta 2(97) (FG4)His---Tyr substitution at the alpha 1-beta 2 interface.
An abnormal profile of cation exchange high performance liquid chromatography for the determination of Hb A1c led to the discovery of a new hemoglobin variant with a His----Tyr substitution at position 97(FG4) of the beta chain. The variant comprised about equal proportion to normal Hb A. It showed an increased oxygen affinity, normal n value and slightly reduced alkaline Bohr effect. However, the magnitude of the functional deviation is less than that of other variants with substitutions at the same position, and the heterozygous carrier was not erythremic.
Department of Clinical Laboratory Science, Yamaguchi University School of Medicine, Ube, Japan.
Y
Y
Ohba
K
K
Imai
I
I
Kumada
A
A
Ohsawa
T
T
Miyaji
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005914:Globins / Q000302:isolation & purification; D006442:Glycated Hemoglobin / Q000032:analysis; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification*; D006579:Heterozygote; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008297:Male; D010100:Oxygen / Q000097:blood; D014357:Trypsin
D000596:Amino Acids; D005914:Globins; D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; D010100:Oxygen; D014357:Trypsin; C060712:hemoglobin Moriguchi