Imaging of human thrombi in the rabbit jugular vein: I: Comparison of two fibrin-specific monoclonal antibodies.
The development of monoclonal antibodies with a specificity for cross-linked fibrin may have a potential role in the detection and of thrombi and thrombolytic therapy. In this study, two monoclonal antibodies with a specificity for fibrin have been examined. In vitro studies have shown NIBn 123 (which has a high affinity for X-oligomer) and DD-3B6 to bind to immobilised fibrin on PVC plates as well as plasma clots which were incubated in the presence of plasma. The Km values for NIBn 123 and DD-3B6 wre 1.0 x 10(10)/7.7 x 10(8) M and 2.6 x 10(8) M respectively. No significant binding to fibrinogen either immobilised or in solution was found. The binding of these antibodies to a human thrombus in the jugular vein of the rabbit was monitored over a 24 hour period. Preferential binding of each antibody reached a ratio of approximately 1.0 (jugular/heart) at 24 hours and an image was detected.
National Institute for Biological Standards and Control, Potters Bar, Herts., UK.
P M
PM
Tymkewycz
L J
LJ
Creighton
P S
PS
Gascoine
G D
GD
Zanelli
P M
PM
Webbon
P J
PJ
Gaffney
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D005337:Fibrin / Q000276:immunology*; D006801:Humans; D007457:Iodine Radioisotopes; D007601:Jugular Veins / Q000000981:diagnostic imaging; D046911:Macromolecular Substances; D011817:Rabbits; D011877:Radionuclide Imaging; D013927:Thrombosis / Q000000981:diagnostic imaging*
D000911:Antibodies, Monoclonal; D000941:Antigens; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances; D005337:Fibrin