Modulation of chromatin structure and histone H1 content of rat intestinal epithelial cells in response to dietary methionine-cysteine restriction.
Rats were fed for 6 days a control diet, a diet low in methionine-cysteine or the diet low in methionine-cysteine followed by 2 days of the control diet. One group was pair-fed the control diet with the food intake of the methionine-cysteine restricted group. The mass of cells obtained from 60 cm of the jejunum decreased significantly after methionine-cysteine restriction, increased after refeeding and was higher in the pair-fed than the methionine-cysteine restricted rats. Sedimentation values showed an increase in amount of chromatin of greater than 100S in the methionine-cysteine restricted restricted as compared with the control chromatin. In the heavy chromatin regions histone H1 in percent of the total proteins was significantly higher in the methionine-cysteine restricted than the control rats, while transcription activity was significantly diminished.
Wenner-Gren Institute for Experimental Biology, University of Stockholm, Sweden.
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M
Norell
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von der Decken
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D003545:Cysteine / Q000008:administration & dosage / Q000172:deficiency*; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004032:Diet; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000172:deficiency*; D009687:Nuclear Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D002843:Chromatin; D006657:Histones; D009687:Nuclear Proteins; D004247:DNA; D008715:Methionine; D012321:DNA-Directed RNA Polymerases; D003545:Cysteine