Effect of human serum on alkaline phosphatase induction in cultured human tumor cells.
The continuous cell lines T 24 and HT-29, derived from human bladder and colon carcinomas, produce term-placental and intestinal alkaline phosphatase, respectively. Growth in hyperosmolar medium or exposure to prednisolone or sodium butyrate induces increased enzyme levels, and combinations of inducers elicit synergistic activity increases. The effect of the inducing agents is strikingly diminished when cells are grown in the presence of high concentrations of human serum, and the synergistic increases are essentially abolished. Major human serum protein fractions do not affect alkaline phosphatase induction.
Department of Pathology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx 10467-2490.
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D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D001769:Blood; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D003110:Colonic Neoplasms / Q000201:enzymology; D004790:Enzyme Induction; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D009994:Osmolar Concentration; D014407:Tumor Cells, Cultured / Q000201:enzymology*; D001749:Urinary Bladder Neoplasms / Q000201:enzymology
D002087:Butyrates; D005938:Glucocorticoids; D020148:Butyric Acid; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase