Structural significance of the C-terminal amphiphilic helix of interleukin-2.
The structural significance of C-terminal amphiphilic alpha-helix of human interleukin-2 has been investigated using principles of protein design. Employing disulfide-mediated semi-synthesis, several multiple residue substitution patterns were studied in order to provide rapid insight into the most appropriate features to incorporate into fully recombinant proteins. Substitutions directed toward both stabilization and destabilization of the helix resulted in proteins with modulated bioactivity. Circular dichroism verified the conformational integrity and thermal stability of the derivatives. The biologic characteristics of each derivative were evaluated in the standard murine CTLL-2 assay and compared to activities exhibited in both human T-cell bioactivity and binding assay. A strategy for the design of protein ligand agonists and antagonists without knowledge of receptor contact residues is discussed.
Department of Pharmacology, Dartmouth Medical School, Hanover, New Hampshire 03756.
B
B
Landgraf
F E
FE
Cohen
K A
KA
Smith
R
R
Gadski
T L
TL
Ciardelli
D001667:Binding, Competitive; D002942:Circular Dichroism; D006801:Humans; D007376:Interleukin-2 / Q000138:chemical synthesis / Q000276:immunology; D007700:Kinetics; D010455:Peptides / Q000138:chemical synthesis; D011487:Protein Conformation; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology
D007376:Interleukin-2; D010455:Peptides; D015375:Receptors, Interleukin-2