Serum IgG antibodies reactive with lipoteichoic acid in adult patients with periodontitis.
IgG antibody levels to lipoteichoic acid (LTA), prepared from Streptococcus mutans cells, were determined by enzyme-linked immunosorbent assay in serum samples from 149 subjects. An extract from Bacteroides gingivalis and lipopolysaccharide from Escherichia coli 055:B5 served as control antigens. The reference group comprised 28 systemically and periodontally healthy adults. The main test groups were: 52 persons with gingivitis only, and 69 patients with periodontitis. Within those groups, 37 patients had insulin-dependent diabetes mellitus, another 20 patients were prospective or renal transplant recipients. The periodontitis patient group showed significantly (p less than 0.05) higher mean antibody value and higher frequency of extreme antibody responses to both LTA and B. gingivalis than the gingivitis group. LPS did not discriminate between the groups. Multiple regression analysis with gingivitis scores as the dependent variable selected plaque scores, anti-LTA antibody values and general health status as significant (p less than 0.05) regressors. The variance in radiographical alveolar bone loss was significantly (p less than 0.05) explained by age and by antibody values to B. gingivalis and to LTA. The patients with extreme immunological responsiveness to LTA or to B. gingivalis had about twice as much alveolar bone loss as those with normal serological reactivity. The results support the contention that LTA modulates the progression of periodontitis in humans.
Department of Periodontology, Dental Faculty, University of Oslo, Norway.
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Monefeldt
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Tollefsen
D000293:Adolescent; D000328:Adult; D000937:Antigen-Antibody Reactions; D001862:Bone Resorption / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005891:Gingivitis / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007165:Immunosuppression Therapy; D007676:Kidney Failure, Chronic / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology* / Q000382:microbiology; D013682:Teichoic Acids / Q000276:immunology*
D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid