A fluorescence histochemical method for the demonstration of central catecholamine neurons in young embryonic tissues.
A simple fluorescence histochemical technique is described for the production of catecholamine histofluorescence in the developing brain of rat embryos. The procedure combines the magnesium-formaldehyde-glutaraldehyde perfusion technique, with polyethylene glycol dehydration and embedding. Several examples illustrate the sensitivity of the technique and the good preservation of tissue sections. The tissue can also be stored for several weeks without an appreciable loss of fluorescence.
Woerly
S
S
Centre de Recherche en Neurobiologie, Hôpital de l'Enfant-Jésus, Québec, Canada.
Marchand
R
R
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology*; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis*; D005453:Fluorescence; D006651:Histocytochemistry / Q000379:methods; D009474:Neurons / Q000032:analysis*; D051381:Rats
D002395:Catecholamines