Protection against Trichinella spiralis induced by purified stage-specific surface antigens of infective larvae.
The stage-specific surface antigens of the infective larvae of Trichinella spiralis, isolated using an affinity column of monoclonal antibody (Mab) NIM-M1, consisted of four components with molecular weights of 72, 65, 52, and 47 kDa, respectively. These four components may have unique as well as shared structural features and appear to be products of the stichosome. When injected i.p. as an emulsion in complete Freund's adjuvant, the purified antigens induced protection against infection of BALB/c mice with T. spiralis, as assessed by reductions in both the muscle larvae load and the number of adult intestinal worms.
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F.
G
G
Ortega-Pierres
E
E
Muñiz
R
R
Coral-Vázquez
R M
RM
Parkhouse
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization; D007814:Larva / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000517:prevention & control*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000954:Antigens, Surface