Radioimmunoassay of 2-hydroxyesterone using antisera raised against antigenic complexes obtained by convenient methods.
Antigenic complexes of 2-hydroxyestrone (2-OHE1) were obtained by Mannich reaction of 2-OHE1 and bovine serum albumin (BSA) and by coupling of 2-OHE1 1-glutathione thioether to BSA using glutaraldehyde. Antiserum raised against the antigen obtained by the Mannich reaction had high affinity (Kd = 3.8 x 10(9) M-1) and relatively high specificity; cross reactivities for estrone, 4-hydroxyestrone and 2-methoxyestrone were 2.1%, 10% and 1.5%, respectively. The other antiserum also had high affinity (4.5 x 10(9) M-1) but its cross reactivities for the above three steroids were more than 100%. Concentrations of 2-hydroxyestrone in human plasma were determined by radioimmunoassay with the more specific antiserum and Sephadex LH-20 chromatography to be less than a minimum detectable amount (less than 10 pg/ml) (men), 20.9 pg/ml (women, proliferation) and 26.0 pg/ml (women, periovulation).
M
M
Numazawa
K
K
Kimura
M
M
Nagaoka
A
A
Kambegawa
D000328:Adult; D000818:Animals; D003429:Cross Reactions; D004970:Estrone / Q000031:analogs & derivatives*; D005260:Female; D006801:Humans; D006894:Hydroxyestrones / Q000032:analysis* / Q000276:immunology; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay
D006894:Hydroxyestrones; D004970:Estrone; C024980:2-hydroxyestrone