Sodium dodecyl sulfate-glycerol polyacrylamide slab gel electrophoresis for the resolution of apolipoproteins.
We describe the resolution of the plasma apolipoproteins with molecular weights from 8,800 to greater than 550,000, using a 3.5% sodium dodecyl sulfate-glycerol polyacrylamide slab gel system. The simplicity of this system and the resolution of proteins over a broad range of molecular weights will make it particularly useful in investigations of apolipoprotein composition of plasma lipoproteins.
Department of Medicine, St. Michael's Hospital, Canada.
G F
GF
Maguire
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M
Lee
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Connelly
D001053:Apolipoproteins / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate
D001053:Apolipoproteins; D012967:Sodium Dodecyl Sulfate