The constitution and properties of phosphorylated and unphosphorylated C-terminal fragments of progastrin from dog and ferret antrum.
Antibodies to the extreme C-terminal tryptic (nona-) peptide fragment of porcine progastrin have been used in radioimmunoassay to identify progastrin fragments in dog, ferret and pig antral mucosa extracts and to monitor their purification. In addition to previously characterised phosphorylated and unphosphorylated C-terminal tryptic peptides of porcine progastrin a minor form corresponding to the C-terminal octapeptide (i.e. des-Ser C-terminal nonapeptide) was isolated and characterised. The latter form together with phosphorylated and unphosphorylated forms of the nonapeptides were also isolated and chemically characterised from dog antrum, and the unphosphorylated nonapeptide was characterised from ferret antrum. The primary amino acid sequences of the dog, ferret and pig nonapeptides were identical. In ferret the unphosphorylated nonapeptide predominated, and in dog the phosphorylated form predominated; in pig both forms of the nonapeptide were well represented. Intact progastrin was identified in gel filtration eluates of extracts of all 3 species, but occurred only in relatively low concentrations. The nonapeptides did not stimulate acid secretion in the conscious gastric fistula rat and they did not modify the acid response to G17. Phosphorylation of progastrin-derived peptides is evidently well conserved across a range of species even though there appear to be differences in the relative proportions of phosphorylated and unphosphorylated forms.
Department of Physiology, University of Liverpool, U.K.
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Desmond
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Varro
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Young
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Gregory
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Nemeth
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GJ
Dockray
D000818:Animals; D002852:Chromatography, Ion Exchange; D004285:Dogs; D005289:Ferrets; D005744:Gastric Acid / Q000378:metabolism; D005755:Gastrins / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011498:Protein Precursors / Q000302:isolation & purification / Q000378:metabolism*; D011706:Pyloric Antrum / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine
D005755:Gastrins; D011498:Protein Precursors; C013706:big gastrin