[Ligase joining of oligodeoxynucleotides on a oligodeoxynucleotide analog matrix containing P-S-C(5')-internucleotide bonds].
Joining of oligodeoxynucleotides by T4 polynucleotide ligase on an oligodeoxynucleotide template analogue having P--S--C(5') internucleotide bonds was investigated. The enzyme did ligate the oligodeoxynucleotides but with an extremely low rate. The major product of the enzymatic reaction proved to be the adenylylated intermediate of the 5'-phosphorylated oligodeoxynucleotide, the product of the second stage of the overall ligation reaction. It is noteworthy that this intermediate is not accumulated in ligase reactions under standard conditions when DNA templates rather than template analogues were used.
V N
VN
Rybakov
V S
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Bogachev
D055598:Chemical Phenomena; D002621:Chemistry; D011088:DNA Ligases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D013379:Substrate Specificity; D013604:T-Phages / Q000201:enzymology; D013698:Templates, Genetic
D009838:Oligodeoxyribonucleotides; D011088:DNA Ligases
Ligaznaia sshivka oligodezoksinukleotidov na matritse--oligodezoksi- nukleotidnom analoge soderzhashchem P-S-C(5')-mezhnukleotidnye sviazi.