Neurotrophic action of leupeptin, a protease inhibitor on ventral spinal cord in culture.
Studies with organotypic culture of ventral spinal cord revealed that leupeptin, a protease inhibitor at 10(-4) to 10(-10) M exerts a marked growth-stimulating effect. The presence of leupeptin, the neuritic extension of ventral spinal cord exceeds control values 1.5-4.9 times. The growth zone of spinal cord explants increases in the leupeptin treated cultures. The possible mechanism of trophic effect of leupeptin on cultured ventral spinal cord was briefly discussed.
Iwasaki
Y
Y
Fourth Department of Internal Medicine, Toho University Ohashi Hospital, Japan.
Kinoshita
M
M
Ikeda
K
K
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D004622:Embryo, Mammalian; D007976:Leupeptins / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D009924:Organ Culture Techniques; D011107:Polylysine; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology* / Q000187:drug effects
D007976:Leupeptins; D009842:Oligopeptides; D011107:Polylysine