Studies on carrageenin air pouch inflammation in the rat.
Inflammation was induced in the 6-day subcutaneous air pouch of the rat by injection of carrageenin. The model was characterized in terms of exudate volume, leucocyte accumulation, granuloma, vascular permeability and protein clearance up to 7 days after injection of carrageenin. From days 2-3 rapid and reproducible changes in these responses were observed which indicated a change from polymorphonuclear (PMN) leucocyte-dominated to mononuclear (MN) leucocyte-dominated inflammation. A second injection of carrageenin on day 3 gave increases in exudate formation and PMN accumulation on day 4. Administration of carrageenin mixed with 3 day inflammatory exudate gave an increased exudate volume and decreased leucocyte accumulation at 6 h. Reduction of 6-h cellular accumulation by use of a lower dose of carrageenin or a I-day air pouch gave complete inhibition of exudate formation on day 3. In contrast, inhibition of the 6-h cell response with prednisolone had no effect on the 3-day response. Daily treatment with indomethacin gave increased PMN accumulation on day 3. Similar treatment with prednisolone additionally reduced exudate volume. Treatment on day 2 with prednisolone gave similar effects whereas indomethacin, BW755C and protease inhibitors had no effect. Administration of colchicine at this time gave inhibition of exudate volume on day 3 whereas complement depletion gave increases in volume and PMNs.
Roussel Laboratories Ltd, Swindon, Wilts, UK.
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P
Hambleton
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P
Miller
D000818:Animals; D002351:Carrageenan; D003877:Dermatitis, Contact / Q000473:pathology*; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D007958:Leukocyte Count / Q000187:drug effects; D008297:Male; D009504:Neutrophils; D011239:Prednisolone / Q000494:pharmacology; D051381:Rats
D002351:Carrageenan; D011239:Prednisolone; D007213:Indomethacin