The tissue distribution of Ia- and IgM-positive cells in adult and newborn miniature pigs.
Distribution of Ia- and IgM-positive cells was compared in tissues of adult and newborn miniature pigs, using indirect immunoperoxidase and immunofluorescent techniques with monoclonal antibodies. The different distribution patterns were found mainly in adult jejunum and kidney. Both IgM- and Ia-staining were ascertained in the lamina propria between the Lieberkühn crypts and in the lymphoid follicles of the jejunum. Moreover, IgM-positive cells were demonstrated in the crypts while Ia-reaction was seen in the lamina propria of the villi. In the kidney, IgM-staining was limited to the glomeruli. On the contrary, a distinct Ia-reaction was found mainly in intertubular structures. Small differences in IgM- and Ia-positive cell distribution were also noted in the mesenteric lymph nodes and in the spleen. Developmental changes of the adult distribution patterns (chiefly that of IgM-positive cells) were found in all tissues studied at 2 days of age.
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Horák
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Hruban
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Dvorák
D000375:Aging / Q000276:immunology*; D000818:Animals; D000831:Animals, Newborn; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D007583:Jejunum / Q000166:cytology / Q000276:immunology*; D007668:Kidney / Q000166:cytology / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013552:Swine; D013556:Swine, Miniature / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D007075:Immunoglobulin M