Chemiluminescent detection of DNA: application for DNA sequencing and hybridization.
A non-radioactive DNA detection chemistry is described and its application is shown for DNA hybridization and standard dideoxy DNA sequencing. The method employes a biotin-streptavidin system which binds an enzyme specifically to a target DNA and upon exposure to substrate, the enzyme catalyzes a chemiluminescent reaction. The image is captured within seconds by a Polaroid or X-ray film. The method is capable of detecting DNA in the hundred attomol range.
Beck
S
S
MilliGen/Biosearch, Division of Millipore, Burlington, MA 01803.
O'Keeffe
T
T
Coull
J M
JM
Köster
H
H
D001483:Base Sequence; D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008832:Microchemistry; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis
D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides