Characterization of the proteoglycogen fraction non-extractable from retina by trichloroacetic acid.
The trichloroacetic acid-insoluble 1,4-alpha-glucan fraction from bovine retina was purified and characterized. It is a proteoglycogen fraction containing a 42 kDa protein moiety similar in size to the protein moiety of the trichloroacetic acid-soluble proteoglycogen fraction. The apparent weight-average Mr of acid-insoluble and acid-soluble proteoglycogens are 4.7 x 10(5) and 7.0 x 10(5) respectively. The present results support suggestions from earlier studies indicating that acid-insoluble proteoglycogen is the precursor of the acid-soluble form.
Department de Quimica Biologica-CIQUIBIC, Facultad de Ciencias Quimicas-CONICET, Cordoba, Argentina.
M C
MC
Miozzo
E R
ER
Lacoste
J A
JA
Curtino
D000818:Animals; D002417:Cattle; D005936:Glucans / Q000302:isolation & purification*; D008970:Molecular Weight; D011509:Proteoglycans / Q000302:isolation & purification*; D012160:Retina / Q000032:analysis*; D012995:Solubility; D014238:Trichloroacetic Acid
D005936:Glucans; D011509:Proteoglycans; D014238:Trichloroacetic Acid; C040088:1,4-glucan