Age-related decline of acetylcholine release evoked by depolarizing stimulation.
Release of endogenous acetylcholine (ACh) from the dorsal hippocampus in response to depolarizing stimulation with high-K+ infusion was examined in young and aged rats using the method of in vivo dialysis. ACh content in the dialysate was determined by high-performance liquid chromatography-electrochemical detection (HPCC-ECD). During the high-K+ stimulation, the concentration of ACh in the dialysate only slightly increased in aged rats in contrast with young rats where the ACh content during stimulation increased about 2-fold of the basal level. These results showed that ACh release evoked by depolarizing stimulation declined through aging in the hippocampus.
Department of Neuroscience, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
N
N
Takei
I
I
Nihonmatsu
H
H
Kawamura
D000109:Acetylcholine / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D005071:Evoked Potentials; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D006651:Histocytochemistry; D007700:Kinetics; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D000109:Acetylcholine; D011188:Potassium