[Pyridoxal phosphate-dependent enzymes of sulfur amino acid metabolism].
Cystathionine beta-synthase and gamma-cystathionase, the two major enzymes of the transsulfuration pathway of methionine metabolism, are described. These enzymes are responsible for inborn errors, e.g., homocystinuria and cystathioninuria. The interaction of gamma-cystathionase with the cofactor, substrates and inhibitors of the general formula RONH2 containing structural fragments of substrates has been studied. A non-radioactive avidin-biotin system for the microdetection of gamma-cystathionase in dot blots has been developed. This system was applied for immunoscreening of a rat liver cDNA library in the prokaryotic expression vector lambda gt 11.
Gabibov
A G
AG
Shuster
A M
AM
Khomutov
A R
AR
Tolosa
E A
EA
Goriachenkova
E V
EV
D000603:Amino Acids, Sulfur / Q000378:metabolism*; D000818:Animals; D003541:Cystathionine beta-Synthase / Q000378:metabolism*; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D008190:Lyases / Q000378:metabolism*; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
D000603:Amino Acids, Sulfur; D011732:Pyridoxal Phosphate; D008190:Lyases; D006836:Hydro-Lyases; D003541:Cystathionine beta-Synthase; D003542:Cystathionine gamma-Lyase
Pirodksal'fosfat-zavisimye fermenty metabolizma serosoderzhashchikh aminokislot.