[Inhibition of acetylcholinesterase at pupil-related central nuclei by organophosphorus pesticide (fenthion)--an experimental study].
An organophosphorus pesticide (OP) inhibits acetylcholinesterase (AChE) of various organs, especially in cholinergically innervated organs. Pupil constriction is a major sign of an ocular symptom in the patient with acute OP intoxication, but the etiology is unknown. The present experiments were intended to elucidate a central mechanism of pupil size in which AChE and OP are involved. Fenthion (dimethyl 4-methylthion-m-tolyl phosphorothionate) of 5mg/day body weight) was subcutaneously administered dorsally in Wistar rats for 14 days consecutively in the longest group. Histochemical staining and microspectrophotometry were used to analyze the intensity of the AChE activity in the iris, the pretectal nucleus and Edinger-Westphal (EW) nucleus on the 2nd, 4th, 6th, 10th and 14th day of administration. The pupil was measured after 20 minutes of dark adaptation. The pupil constricted on the 6th day and most remarkably on the 10th day. The AChE activity in the iris decreased remarkably on the 4th day. Miosis was not seen on the 4th day. The AChE activity in the pretectal nucleus decreased remarkably on the 6th day. The AChE activity in the EW nucleus reduced gradually from the 6th day and 62% reduction of initial value was seen on the 14th day. Intense miosis was seen on the 10th day. In conclusion inhibition of AChE activity was detected first in the iris, thereafter in the pretectal nucleus and the EW nucleus in close correlation with the miotic pupil. Therefore, miosis associated with OP intoxicated patients may parallel the central inhibition of AChE in pupil-related centers.
Hayakawa
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S
Hiramoto
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D
Sekiya
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H
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D003251:Constriction, Pathologic / Q000139:chemically induced; D005284:Fenthion / Q000633:toxicity*; D007498:Iris / Q000201:enzymology; D008297:Male; D008916:Miotics; D011680:Pupil / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D012025:Reflex, Pupillary / Q000187:drug effects
D002800:Cholinesterase Inhibitors; D008916:Miotics; D005284:Fenthion; D000110:Acetylcholinesterase