Effect of clomiphene on nuclear estrogen receptor of the fallopian tube during ovum transport in rabbits.
The effect of clomiphene on nuclear estrogen receptors of the Fallopian tube during ovum transport in the rabbit has been studied. Nuclear binding capacity was observed in ampulla (A), ampullary-isthmic junction (AIJ), isthmus (I), uterine-isthmic junction (UIJ) and uterus (U). Receptor concentration decreased in all segments of the tube after administration of clomiphene in mated animals. The bindings are of high affinity and low capacity. Important alterations were observed during transport when compared to that of 14, 24, 34, 48, 72, 144 and 168 hr post-coitum (p.c). At 24 hr p.c binding increased only in I and decreased in A and AIJ. Retention of eggs at I at 24 hr p.c showed as increase in binding at I. Egg transport was accelerated and eggs reached prematurely in the uterus due to the influence of clomiphene. Binding in I remained constant from 48 hr p.c to 144 hr p.c but concurrently the binding level increased in U from 34 hr p.c. The elevation of nuclear estrogen receptor level was maximum at 24 hr p.c which coincided with increased plasma estrogen level. The result of such study showed that clomiphene depleted nuclear estrogen receptor complex in the fallopian tube before transfer to the uterus. Further, observation indicated that clomiphene acted directly on the rate of egg transport because of the variations in estrogen receptors during different time periods. Thus, clomiphene reduced the quantity of estrogen receptor i.e., insensitiveness to estrogen. The variations in estrogen binding to its receptor and plasma level at different post-coital periods are modulated by clomiphene resulting in the acceleration of egg transport and prevention of pregnancy.
Gupta
J S
JS
Division of Endocrinology, Central Drug Research Institute, Lucknow, India.
Roy
S K
SK
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002996:Clomiphene / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism; D005187:Fallopian Tubes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005260:Female; D007700:Kinetics; D010066:Ovum Transport; D011817:Rabbits; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*; D012016:Reference Values
D011960:Receptors, Estrogen; D002996:Clomiphene; D004958:Estradiol