Determination of the sex of chickens by a biotin-labeled deoxyribonucleic acid probe.
Repeating units of the W chromosome-specific, XhoI-family repetitive DNA of chickens have been cloned and sequenced. The recombinant plasmid carrying the .7 kilobase (kb) W chromosome-specific repeating unit was labeled by biotinylated deoxyuridine 5'-triphosphate and used to determine the sex of White Leghorn chicken by two DNA hybridization techniques. Hybrids formed were detected by the streptavidin-alkaline phosphatase method. By Southern blot hybridization, XhoI-.7 kb, XhoI-1.1 kb, and EcoRI-1.3 kb fragments were detected in the genomic DNA from the female White Leghorn but not in the DNA from the male. By dot-blot hybridization, which is the most convenient way to detect a specific nucleotide sequence, the difference between male and female could be clearly detected not only using purified DNA samples but also using blood cells or even blood itself as samples.
Uryu
N
N
Research Institute, Nichirei Corporation, Tokyo, Japan.
Nagata
Y
Y
Ito
K
K
Saitoh
H
H
Mizuno
S
S
D000818:Animals; D001710:Biotin; D015139:Blotting, Southern / Q000662:veterinary; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D005260:Female; D015151:Immunoblotting / Q000662:veterinary; D008297:Male; D012091:Repetitive Sequences, Nucleic Acid; D012732:Sex Determination Analysis / Q000662:veterinary*
D015342:DNA Probes; D001710:Biotin; D004247:DNA