[The fluorimetric determination of dabequin in biological fluids].
A study of dabequin was performed to define its possible quantification by spectrofluorimetry. By this technique, dabequin was shown to present maximum excitation and emission wave length at 400 and 450 nm, respectively. Drug emission was determined at different concentrations of sulfuric acid and no changes were observed. Two quantification procedures are compared to determine, which one produces better results in quantifying total serum and blood levels in patients. Specificity of a thin-layer chromatography method, as well as the drug stability in 0.05 M sulfuric acid medium are shown.
E
E
Muñoz
R
R
Tapanes
G
G
Perez
M
M
Febles
F
F
Ramos
D000634:Aminoquinolines / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics; D000962:Antimalarials / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics; D001826:Body Fluids / Q000032:analysis* / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D005069:Evaluation Studies as Topic; D006801:Humans; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
D000634:Aminoquinolines; D000962:Antimalarials; D012996:Solutions; C015773:dabequin
Fluorimetricheskoe opredelenie dabekhina v biologicheskikh zhidkostiakh.