Further studies on the effect of ASF factor on Cl- permeability across the Deiters' neurone plasma membrane.
Experiments have been performed in order to assess whether the antisecretory factor (ASF) can influence the permeation of Cl- ions across the Deiters' neurone plasma membrane in the outward direction. ASF is a naturally occurring, acidic protein with a molecular weight of about 60,000, which specifically inhibits enterotoxin-induced intestinal secretion. Both basal and "intracellular" GABA activated Cl- permeability was studied, and the result confirmed the existence of GABAA receptors on the Deiters' membrane cytoplasmic side. ASF completely abolished the effect of "intracellular" GABA on Cl- permeability in the outward direction. However, ASF did not influence the basal Cl- permeability in the outward direction.
Centro di Neurofisiologia Cerebrale, C.N.R., Genova, Italy.
M V
MV
Rapallino
A
A
Cupello
S
S
Lange
I
I
Lönnroth
H
H
Hydén
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D003984:Dibenzazepines / Q000494:pharmacology; D018756:GABA Antagonists; D066298:In Vitro Techniques; D007700:Kinetics; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology*; D010539:Permeability; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D002712:Chlorides; D003984:Dibenzazepines; D018756:GABA Antagonists; D009479:Neuropeptides; C049628:antisecretory factor; D005680:gamma-Aminobutyric Acid; C041662:pitrazepin