Reversed-phase HPLC separation of plasma norepinephrine, epinephrine, and dopamine, with three-electrode coulometric detection.
We present a method involving minor modifications of previous techniques, in which we use an ion-pair reversed-phase HPLC separation with three-electrode coulometry. Isocratic baseline separations can be obtained in 5 min. The sample preparation procedure, involving extraction with alumina at low temperature, allows good reproducibility (within-run and between-run CVs less than 10%) and improved sensitivity (less than 5 pg of each catecholamine per extract). This method allows approximately 70 low-cost plasma catecholamine analyses to be done in a working day.
Cattedra Patologia Speciale Medica R, Ospedale San Martino, Universita' di Genova, Italy.
N R
NR
Musso
C
C
Vergassola
A
A
Pende
G
G
Lotti
D002395:Catecholamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000097:blood; D004563:Electrochemistry; D004566:Electrodes; D004837:Epinephrine / Q000097:blood; D006801:Humans; D009638:Norepinephrine / Q000097:blood
D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine