Production and secretion of recombinant soluble CD3 polypeptides by myeloma-derived transfectant clones.
Soluble forms of three human CD3 proteins have been produced by recombinant DNA techniques. The extracellular domain of CD3-gamma, -delta or -epsilon has been linked to the constant region of mouse immunoglobulin kappa light chain to form gamma-kappa, delta-kappa and epsilon-kappa chimaeric proteins. These are secreted by mouse myeloma-derived transfectant cell lines and are immunoprecipitable by CD3- or kappa-specific polyclonal antisera. Yields of 100-500 micrograms secreted recombinant proteins per litre of culture medium were obtained, which could be purified by anti-kappa affinity chromatography. The production of soluble CD3 illustrates the applicability of this technology to a loosely associated protein complex.
Basel Institute for Immunology, Switzerland.
A
A
Tunnacliffe
C
C
Olsson
A
A
Traunecker
G W
GW
Krissansen
K
K
Karjalainen
D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000378:metabolism; D015153:Blotting, Western; D002460:Cell Line; D002678:Chimera; D002999:Clone Cells; D010957:Plasmids; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014162:Transfection
D000943:Antigens, Differentiation; D011994:Recombinant Proteins