[The determination of trypsin activity in duodenal fluid with N alpha-(p-toluenesulfonyl)-L-arginine-p-nitranilide (L-TAPA)].
The conditions are optimized for the determination of trypsin activity in duodenal juice at 37 degrees C using L-TAPA as a substrate ("Bio-La-Test Trypsin", Lachema, CSSR). Most of the directions of the kit could be accepted, but optimal pH was 0.4 units below that described there. This method allows a highly specific, sensitive, and sufficiently precise determination of trypsin activity in duodenal juice.
Siegmund
E
E
Dummler
W
W
D000813:Anilides / Q000378:metabolism*; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D004386:Duodenum / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic / Q000592:standards; D013379:Substrate Specificity; D014357:Trypsin / Q000032:analysis*
D000813:Anilides; D011933:Reagent Kits, Diagnostic; C009507:N(alpha)-tosylarginine-4-nitroanilide; D001120:Arginine; D014357:Trypsin
Die Bestimmung der Trypsinaktivität im Duodenalsaft mit N alpha-(p-Toluensulfonyl)-L-arginin-p-nitranilid (L-TAPA).