Acarbose is a competitive inhibitor of mammalian lysosomal acid alpha-D-glucosidases.
Intraperitoneal injections (approximately 400 mg/kg of body weight) of acarbose, an inhibitor of acid (1----4)-alpha-D-glucosidase, perturb the metabolism of glycogen in the liver, resulting in excess storage of lysosomal glycogen. The metabolism of skeletal muscle glycogen was unaffected, suggesting that acarbose either does not enter the tissue or that the muscle alpha-D-glucosidase is not inhibited. The hydrolysis of maltose and glycogen by the acid alpha-D-glucosidases from rat liver, rat skeletal muscle, and human placenta was inhibited competitively by acarbose. Thus, the lack of effect of acarbose upon the metabolism of muscle glycogen is due to its inability to enter the tissue.
Calder
P C
PC
Department of Biochemistry, University of Auckland, New Zealand.
Geddes
R
R
D020909:Acarbose; D000818:Animals; D001667:Binding, Competitive; D004791:Enzyme Inhibitors; D005959:Glucosidases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology*; D008958:Models, Molecular; D009132:Muscles / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014312:Trisaccharides / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D014312:Trisaccharides; D006003:Glycogen; D005959:Glucosidases; D006026:Glycoside Hydrolases; D020909:Acarbose