Liposome-mediated delivery of gadolinium-diethylenetriaminopentaacetic acid to hepatic cells: a P-31 NMR study.
Delivery of gadolinium-diethylenetriaminopentaacetic acid (Gd-DTPA) (a magnetic resonance imaging contrast agent) to hepatic cells via liposomes used as carriers was demonstrated and monitored by P-31 NMR spectroscopy at 162 MHz on a perfused rat liver model. The penetration of Gd-DTPA into hepatic cells was reflected by a 41% reduction in the longitudinal relaxation time of the beta phosphorus atom of intrahepatic ATP used as an index of the contrast agent presence. The perfusion of free Gd-DTPA did not affect the relaxation index nor did liposomes devoid of paramagnetic agent. There was no indication of interference of Gd-DTPA, free or encapsulated in liposomes, with liver energetic metabolism. Alterations of hepatic magnetic resonance imaging signal induced by Gd-DTPA containing liposomes might help to assess the functional state of the parenchyma and pinpoint the intracellular compartment as opposed to the intravascular space.
Centre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique (URA No. 1186), Faculté de Médecine Timone, Marseille, France.
J
J
Vion-Dury
S
S
Masson
J M
JM
Devoisselle
M
M
Sciaky
F
F
Desmoulin
S
S
Confort-Gouny
D
D
Coustaut
P J
PJ
Cozzone
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D019786:Gadolinium DTPA; D066298:In Vitro Techniques; D008081:Liposomes; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004369:Pentetic Acid / Q000008:administration & dosage* / Q000493:pharmacokinetics; D010477:Perfusion; D051381:Rats
D008081:Liposomes; D009942:Organometallic Compounds; D004369:Pentetic Acid; D000255:Adenosine Triphosphate; D019786:Gadolinium DTPA