Efficient endotoxin removal with a new sanitizable affinity column: Affi-Prep Polymyxin.
A new affinity column packing for removal of endotoxins has been prepared by coupling USP drug-quality polymyxin B to Affi-Prep, a novel synthetic macroporous polymer. Affi-Prep Polymyxin binds endotoxins from a number of different strains of gram-negative bacteria. Endotoxin binding is not significantly affected by 10 mg/ml of bovine serum albumin or human immunoglobulin G, by 1 mg/ml sodium dodecyl sulphate, or by 1 mg/ml deoxycholate. Affi-Prep Polymyxin is stable to treatment with 1.0 M sodium hydroxide, an important property for sanitizing the resin. The resin shows a high ligand stability, since no leakage of polymyxin B from the packing could be detected. Affi-Prep Polymyxin exhibited the highest endotoxin binding efficiency when compared with several commercial agarose affinity packings.
Talmadge
K W
KW
Bio-Rad Laboratories, Richmond, CA 94801.
Siebert
C J
CJ
D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D003902:Detergents; D004731:Endotoxins / Q000302:isolation & purification* / Q000378:metabolism; D007202:Indicators and Reagents; D011112:Polymyxin B; D011506:Proteins / Q000378:metabolism; D012972:Sodium Hydroxide / Q000494:pharmacology
D003902:Detergents; D004731:Endotoxins; D007202:Indicators and Reagents; D011506:Proteins; D012972:Sodium Hydroxide; D011112:Polymyxin B