Oesophagostomum radiatum: successful vaccination of calves with high molecular weight antigens.
Extracts of adult Oesophagostomum radiatum were resolved into four fractions by gel filtration chromatography on a Superose 12 column. ELISA assays on these four fractions showed that the antibodies produced by naturally infected calves predominantly reacted with the void volume fraction (VV). Three trials were conducted in which calves were vaccinated with the VV fraction of adult extract. When compared to untreated controls, vaccination with the VV of adult extract significantly (P less than 0.05) reduced worm establishment and faecal egg output after challenge infection relative to untreated controls.
East
I J
IJ
Berrie
D A
DA
Fitzgerald
C J
CJ
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D008297:Male; D009814:Oesophagostomiasis / Q000517:prevention & control / Q000662:veterinary*; D009815:Oesophagostomum / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth