Involvement of protein kinase A and casein kinase II in the in vivo protein kinase activities in prophase arrested Xenopus oocytes.
In vivo beta casein phosphorylation was analysed in Xenopus full-grown oocytes arrested in the prophase of the meiotic cell division. The phosphorylation was inhibited by the protein kinase inhibitor (PKI) and also by heparin (3 micrograms/ml; final concentration). beta casein phosphorylation was increased by spermine (2 mM). Therefore, protein kinase A and casein kinase II are both active in vivo in full-grown oocytes and may be involved in the prophase arrest of meiotic cell division.
Physiologie de la Reproduction, CNRS URA 004, Paris.
P
P
Cormier
O
O
Mulner-Lorillon
R
R
Ozon
R
R
Bellé
D000818:Animals; D047388:Casein Kinases; D002364:Caseins; D005260:Female; D009865:Oocytes / Q000166:cytology / Q000201:enzymology*; D010766:Phosphorylation; D011418:Prophase; D011494:Protein Kinases / Q000378:metabolism*; D013379:Substrate Specificity; D014982:Xenopus laevis
D002364:Caseins; D011494:Protein Kinases; D047388:Casein Kinases