Isolation and characterization of a cDNA clone encoding the lima bean lectin.
The complete amino acid sequence of the lima bean (Phaseolus lunatus) lectin was deduced from the nucleotide sequence of a cDNA clone. The lectin appears to be synthesized as a prepeptide consisting of a signal sequence of 21 residues and a mature protein of 241 amino acids. Comparison of the lima bean lectin sequence to the sequences of other leguminous seed lectins indicates regions of extensive homology. Northern blot analysis showed absence of lectin mRNA in the leaves, roots, or stems of 16-day-old lima bean plants.
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109-0606.
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Imbrie-Milligan
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Datta
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Goldstein
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D007887:Fabaceae / Q000235:genetics*; D037102:Lectins / Q000235:genetics*; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000235:genetics; D037121:Plant Lectins; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid
D037102:Lectins; D010835:Phytohemagglutinins; D037121:Plant Lectins; C031887:lima bean lectin; D004247:DNA