[Changes in the composition of DNA-bound nonhistone protein in the thymus and liver of gamma-irradiated rats].
A supramolecular DNA complex (SC DNA) and DNA of a phenol nuclear matrix (PNM DNA) were extracted, by the phenol method, from rat thymus and liver 15 min following 10 Gy gamma-irradiation. The method of electrophoresis in polyacrylamide gel was used to study a composition of nonhistone proteins firmly bound to these DNA fractions. Irradiation was shown to induce the occurrence of new proteins and redistribution of proteins between SC DNA and PNN DNA of rat organs.
N B
NB
Strazhevskaia
V A
VA
Struchkov
D Iu
DIu
Blokhin
D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000528:radiation effects*; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004591:Electrophoresis, Polyacrylamide Gel; D005720:Gamma Rays; D008099:Liver / Q000378:metabolism / Q000528:radiation effects*; D046911:Macromolecular Substances; D008297:Male; D011485:Protein Binding / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000378:metabolism / Q000528:radiation effects*; D013997:Time Factors
D002868:Chromosomal Proteins, Non-Histone; D046911:Macromolecular Substances; D004247:DNA
Izmenenie sostava DNK-sviazannogo negistonovogo belka v timuse i pecheni gamma-obluchennykh krys.