No evidence for a circadian rhythm of protein synthesis in the rat suprachiasmatic nuclei.
The mammalian suprachiasmatic nuclei contain an endogenous circadian pacemaker. A quantitative autoradiographic tracer method that measures L-[1-14C]leucine incorporation into protein was used to determine whether the overall rate of protein synthesis in the nuclei varies in a circadian fashion. Unlike the robust circadian rhythms of electrical activity and energy metabolism previously recorded from the nuclei at the two time points sampled, protein synthesis remained constant.
Scammell
T E
TE
Department of Neurology, University of Massachusetts Medical School, Worcester 01655.
Schwartz
W J
WJ
Smith
C B
CB
D000596:Amino Acids / Q000097:blood; D000818:Animals; D002940:Circadian Rhythm; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*
D000596:Amino Acids; D009419:Nerve Tissue Proteins