Effect of processing time on the quality of mouse bone-marrow metaphase preparations.
To evaluate the effect of extended incubation in phosphate-buffered saline (PBS) on the quality of mouse bone-marrow metaphase preparations, bone-marrow samples were allowed to stand in PBS at room temperature for from 15 min to 3 h before hypotonic treatment and fixation. The frequencies of sister-chromatid exchanges, the rate of cellular proliferation and the mitotic indices in these mice were not significantly affected by prolonged storage in PBS. However, slide quality decreased significantly in bone-marrow samples stored in PBS for more than 2 h. These data demonstrate that the storage of bone-marrow samples in PBS at room temperature for at least 1 h does not adversely affect the quality of routine cytogenetic data, making it more feasible to conduct studies involving large numbers of animals.
Luke
C A
CA
Medical Department, Brookhaven National Laboratory, Upton, NY 11973.
Tice
R R
RR
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure*; D002021:Buffers / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D007621:Karyotyping / Q000379:methods*; D008297:Male; D051379:Mice; D008940:Mitotic Index; D009152:Mutagenicity Tests / Q000379:methods*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology; D013048:Specimen Handling; D013997:Time Factors
D002021:Buffers; D012965:Sodium Chloride