Attenuation of ischemic renal injury with fructose 1,6-diphosphate.
Fructose 1,6-diphosphate (FDP) has been shown to attenuate tissue injury associated with ischemia and shock by enhancing the anaerobic carbohydrate utilization and by inhibiting oxygen-free-radical generation by the neutrophils. Previously, we have reported that FDP prevents ischemic renal failure if administered prior to the ischemic insult. The present study was designed to determine whether this agent could prevent renal damage when administered during the postischemic reperfusion period. Rats were subjected to 30 min of bilateral renal artery occlusion and infused with FDP (350 mg/kg body wt) beginning 10 min after release of the renal artery clamps. Control rats received an equal volume of glucose/saline solution. A third group of rats were sham operated. Twenty-four hours after injury, BUN, creatinine, and fractional sodium excretion values were less in FDP-treated rats than in control rats (P less than 0.001, P less than 0.005, and P less than 0.001, respectively) and not different from values observed in sham-operated rats. Inulin clearance was greater (P less than 0.001) in FDP-treated rats than in control rats (665 +/- 38 microliters/min/g kidney wt). Renal histology was also better preserved in the FDP-treated group. These data suggest that FDP infused after the initiation of an acute ischemic insult provides significant, but not complete, functional and histologic protection from renal damage.
Department of Surgery, University of Mississippi Medical Center, Jackson 39216-4505.
R
R
Didlake
K A
KA
Kirchner
J
J
Lewin
J D
JD
Bower
A K
AK
Markov
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005635:Fructosediphosphates / Q000627:therapeutic use*; D006598:Hexosediphosphates / Q000627:therapeutic use*; D007444:Inulin / Q000378:metabolism; D007511:Ischemia / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007677:Kidney Function Tests; D008297:Male; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000517:prevention & control*
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D007444:Inulin; C029063:fructose-1,6-diphosphate