Protective effect of glutathione against oxygen-induced growth inhibition of human diploid fibroblasts.
The reduced glutathione level in human diploid fibroblasts was increased by the addition of N-acetylcysteine or reduced glutathione ethylester into the culture medium, while it was decreased by the addition of L-buthionine-(R,S)-sulfoximine or diethyl maleate. The hyperbaric oxygen-induced reduction in colony-forming ability was prevented in the N-acetylcysteine- or reduced glutathione ethylester-treated cells, and enhanced in the L-buthionine-(R,S)-sulfoximine- or diethyl maleate-treated cells. The extent of the growth inhibition is well correlated with the cellular glutathione level. It is deduced that glutathione is an important safeguard against the oxygen-induced growth inhibition of human diploid cells.
Honda
S
S
Tokyo Metropolitan Institute of Gerontology, Japan.
Matsuo
M
M
D019328:Buthionine Sulfoximine; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008298:Maleates / Q000494:pharmacology; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D010100:Oxygen / Q000037:antagonists & inhibitors* / Q000633:toxicity
D003470:Culture Media; D008298:Maleates; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; C014476:diethyl maleate; D005978:Glutathione; D010100:Oxygen