Isolation and characterization of dominant, pleiotropic drug-resistance mutants in Chlamydomonas reinhardtii.
Three independent pleiotropic drug-resistance (pdr) mutants were isolated by selecting for resistance to the anti-microtubule herbicides amiprophos-methyl (APM) and oryzalin (ORY). These three mutants and a previously isolated mutant, ani1 (anisomycin resistance), were semi-dominant in heterozygous diploids, and they displayed varying degrees of resistance to structurally and functionally unrelated inhibitors such as cycloheximide, cryptopleurine, emetine, atrazine, and nonidet P-40. Linkage analysis and genetic mapping suggested that three of the four mutants, including ani1, define a single locus, here named pdr1. The fourth mutant defined a new locus, pdr2, which is located on the left arm of linkage group VI. One pdr1 mutant exhibited unusual genetic interactions, including enhanced ts-lethality and synergistic increases in drug resistance, when combined with pdr2-1 and with herbicide-resistant alleles of three other genes.
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108-1095.
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James
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Lefebvre
D000841:Anisomycin / Q000494:pharmacology; D002696:Chlamydomonas / Q000187:drug effects / Q000235:genetics*; D003433:Crosses, Genetic; D004136:Dinitrobenzenes / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005799:Genes, Dominant; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D006540:Herbicides / Q000494:pharmacology; D009154:Mutation; D009578:Nitrobenzenes; D009946:Organothiophosphorus Compounds / Q000494:pharmacology; D010641:Phenotype; D013424:Sulfanilamides; D014170:Transformation, Genetic
D004136:Dinitrobenzenes; D006540:Herbicides; D009578:Nitrobenzenes; D009946:Organothiophosphorus Compounds; D013424:Sulfanilamides; C017823:amiprophos methyl; C012465:oryzalin; D000841:Anisomycin