Enzyme-linked immunosorbent assay for free thyroxin in human serum.
We describe a simple enzyme-linked immunosorbent assay (ELISA) in which microtiter plates are used for determining the free thyroxin concentration (FT4) in serum. Only commercially available chemicals and reagents are needed, including the antiserum. The working range is 1 to 60 ng/L. Turnaround time is about 4 h. Within-run coefficients of variation (CV) for FT4 concentrations of 9.0, 19.0, and 35.0 ng/L were less than 5%; between-run CVs were 10% to 11%. Results by a radioimmunoassay method for 150 sera correlated well (r = 0.922).
Chui
S H
SH
Department of Health Sciences, Hong Kong Polytechnic, Kowloon.
Wan
K C
KC
Lam
C W
CW
Lewis
W H
WH
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011863:Radioimmunoassay; D012016:Reference Values; D013974:Thyroxine / Q000097:blood*
D013974:Thyroxine