Glutathione S-transferases and chloroform toxicity in streptozotocin-induced diabetic rats.
The glutathione S-transferase activity in liver and kidney cytosol was significantly decreased in short term diabetes induced with streptozotocin, whereas no decrease in the transferase was observed in phenobarbital-treated diabetic rats. Toxicity of chloroform was potentiated in streptozotocin- or phenobarbital-treated rats. The decrease in liver cytosolic and microsomal glutathione S-transferase activity was observed in long term diabetic rats, and only microsomal transferase activity was restored by insulin treatment. There was no release of glutathione S-transferases into the serum in the diabetic rats, and the transferases were not inhibited by streptozotocin in vitro. These results showed that glutathione S-transferase activity decreased during diabetes, and this decrease may contribute to altering drug metabolism and toxicity in diabetes.
Aniya
Y
Y
Laboratory of Physiology and Pharmacology, School of Health Sciences, University of the Ryukyus, Okinawa, Japan.
Ojiri
Y
Y
Sunagawa
R
R
Murakami
K
K
Zhenzhong
G
G
Mimura
G
G
Sakanashi
M
M
D000410:Alanine Transaminase / Q000097:blood; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002725:Chloroform / Q000633:toxicity*; D003600:Cytosol / Q000201:enzymology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000503:physiopathology*; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D002725:Chloroform; D000815:Aniline Hydroxylase; D005982:Glutathione Transferase; D000410:Alanine Transaminase