Molecular cloning and nucleotide sequence of the genome of hog cholera virus.
A cDNA clone derived from genomic RNA of hog cholera virus (HCV) was identified using an oligonucleotide complementary to the RNA encoding a hexapeptide from the putative RNA-dependent RNA polymerase of the closely related bovine viral diarrhea virus (BVDV). This clone served as a probe for screening different size-selected cDNA libraries. After molecular cloning and nucleotide sequencing the HCV genome was shown to consist of 12,284 nucleotides containing one long open reading frame. Sequence comparison revealed a high degree of homology between HCV and BVDV genomic RNAs. With respect to HCV the genome of BVDV contains an insertion coding for 90 amino acids.
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
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Meyers
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Rümenapf
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HJ
Thiel
D000595:Amino Acid Sequence; D001483:Base Sequence; D006692:Classical Swine Fever Virus / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping
D012367:RNA, Viral; D004247:DNA; D012321:DNA-Directed RNA Polymerases