Purification of erythropoiesis-stimulating activity (ESA) from one Actinomycetes strain.
An Actinomycetes strain was found to produce a protein which stimulated the growth of murine erythroid progenitors in vitro. The protein was purified to homogeneity and was named Erythropoiesis-Stimulating Activity (ESA). ESA was an acidic glycoprotein with a molecular weight around 80,000. When mouse bone marrow cells were cultured in methylcellulose medium containing physiological concentrations of erythropoietin (0.048 units/ml), addition of ESA stimulated the growth of colony forming unit-erythroid (CFU-E) up to three-fold a in dose dependent manner. ESA was not active on the other hematopoietic progenitors.
Fermentation Research Institute, Tsukuba, Japan.
M
M
Sugie
S
S
Oka
H
H
Tanaka
D000191:Actinomycetaceae / Q000378:metabolism*; D000818:Animals; D001854:Bone Marrow Cells; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004920:Erythropoiesis; D006023:Glycoproteins / Q000302:isolation & purification*; D008222:Lymphokines / Q000302:isolation & purification*; D008297:Male; D051379:Mice; D008970:Molecular Weight
D006023:Glycoproteins; D008222:Lymphokines