Covalent peptide transfer to cell membrane proteins (peptidyl transferase).
HL60 cells, rabbit peritoneal granulocytes or membrane preparations of these cells incorporate radioactivity when reacted with the radioactive peptide tuftsin [3H Pro3]-Thr-Lys-Pro-Arg. The radioactivity which is not diminished by repeated treatments with TCA and NaOH, is covalently bound to a membrane acceptor protein of 100 kDa. The peptide is not displaced by large concentrations of its constituent amino acids. The acceptor protein is resolved into one labeled peak by gel filtration on Sephadex G-200, Sephacryl S-300 and by SDS-PAGE. Digestion by trypsin and chymotrypsin results in the production of smaller fragments.
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
V A
VA
Najjar
N J
NJ
Bump
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010458:Peptidyl Transferases / Q000378:metabolism*; D011817:Rabbits; D014405:Tuftsin / Q000378:metabolism*
D008565:Membrane Proteins; D000217:Acyltransferases; D010458:Peptidyl Transferases; D014405:Tuftsin