Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. VI. Design, chromatographic performance and application of non-porous silica-based anion exchangers.
The linear solvent strength model of Snyder was applied to describe fast protein separations on 2.1-micron non-porous, silica-based strong anion exchangers. It was demonstrated on short columns packed with these anion exchangers that (i) a substantially higher resolution of proteins and nucleotides was obtained at gradient times of less than 5 min than on porous anion exchangers; (ii) the low external surface area of the non-porous anion exchanger is not a critical parameter in analytical separations and (iii) microgram-amounts of enzymes of high purity and full biological activity were isolated.
Jilge
G
G
Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität, Mainz, F.R.G.
Unger
K K
KK
Esser
U
U
Schäfer
H J
HJ
Rathgeber
G
G
Müller
W
W
D001704:Biopolymers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000032:analysis; D046911:Macromolecular Substances; D008433:Mathematics; D009711:Nucleotides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D001704:Biopolymers; D004798:Enzymes; D046911:Macromolecular Substances; D009711:Nucleotides; D011506:Proteins