[Binding of K-18 on the surface of tumor cells--flowcytometrical analysis in vitro].
After K-18 was contacted with various kinds of tumor cell lines in vitro, the binding pattern was examined. K-18 bound on the surface of K-562 cells showed the maximum levels 1 hr later and maintained the binding activity for 24 hrs in the analysis by flowcytometry. K-18 also bound to HeLa S3, KB, RPMI8226, P815, YAC-1 and L1210 cells. Fluorescent intensity on K-562 cells was stronger with whole molecules of K-18 than with F(ab')2 conjugated with melphalan. Normal human IgG could bind more on the surface of K-562 cells than F(ab')2 of IgG. These results suggest that K-18 might bind on tumor cells via IgG Fc receptors on their surface.
Kato
H
H
1st Dept. of Internal Medicine, Kyoto Prefectural University of Medicine.
Yamamura
Y
Y
Tanigawa
M
M
Sano
H
H
Kin
R
R
Inoue
M
M
Sugino
S
S
Kondo
M
M
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007074:Immunoglobulin G / Q000378:metabolism; D008558:Melphalan / Q000378:metabolism; D051381:Rats; D011961:Receptors, Fc / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc; C051713:antineoplastic agent K 18; D008558:Melphalan