Two better cell lines for making hybridomas expressing specific T cell receptors.
Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be studied at a clonal level, without interference of the BW5147-derived receptor chains. In this study one of the variants has been useful in identifying the reactivity to allogeneic MHC Ag of BW5147 itself.
White
J
J
Howard Hughes Medical Institute, Department of Pediatrics, Denver, CO.
Blackman
M
M
Bill
J
J
Kappler
J
J
Marrack
P
P
Gold
D P
DP
Born
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002459:Cell Fusion / Q000379:methods; D002460:Cell Line; D002872:Chromosome Deletion; D006825:Hybridomas / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009154:Mutation; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D013945:Thymoma / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D011948:Receptors, Antigen, T-Cell