Purification of synthetic oligonucleotides on a weak ion-exchange column.
A weak ion-exchange column (PVDI 4000-5) was used to purify oligonucleotides of relatively large size. The purification of polynucleotides was very rapid, they were separated according to size and retained all their properties relevant to genetic engineering experiments.
CRBM du CNRS, U-249 INSERM, Montpellier, France.
J
J
Liautard
C
C
Ferraz
J
J
Sri Widada
J P
JP
Capony
J P
JP
Liautard
D002852:Chromatography, Ion Exchange; D009841:Oligonucleotides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D009841:Oligonucleotides