Repair of a synthetic abasic site in DNA in a Xenopus laevis oocyte extract.
Covalently closed circular DNA containing a synthetic analog of an abasic site at a unique position was used as a substrate to study DNA repair. Incubation of this DNA in Xenopus laevis oocyte extracts resulted in rapid cleavage of the DNA at the abasic site by a class II apurinic-apyrimidinic endonuclease, followed by complete repair within 40 min. Nicked circular DNAs persisted for several minutes before repair by an ATP-dependent DNA synthesis reaction. The repair-related DNA synthesis was localized within 3 or 4 nucleotides surrounding the abasic site. These results are consistent with the short-patch repair reported for DNA damage at heterogeneous sites in human cells (J. D. Regan and R. B. Setlow, Cancer Res. 34:3318-3325, 1974).
Matsumoto
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Y
Department of Pharmacological Sciences, State University of New York, Stony Brook 11794.
Bogenhagen
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DF
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000096:biosynthesis; D004249:DNA Damage; D004260:DNA Repair; D004270:DNA, Circular / Q000378:metabolism; D005260:Female; D005663:Furans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D014982:Xenopus laevis / Q000378:metabolism
D004270:DNA, Circular; D005663:Furans; C018674:tetrahydrofuran; D004247:DNA