Structure of the capsular polysaccharide of Klebsiella ozaenae serotype K4 containing 3-deoxy-D-glycero-D-galacto-nonulosonic acid.
The acidic capsular polysaccharide from the museum strain 2211 of Klebsiella ozaenae serotype K4 is built up of pentasaccharide repeating-units that contain residues of D-glucose, D-mannose, D-glucuronic acid, and 3-deoxy-D-glycero-D-galacto-nonulosonic acid (Kdn) in the ratios 2:1:1:1, as well as an O-acetyl group. The last-named sugar, which is reported in bacterial polysaccharides for the first time, was identified as the methyl (methyl 3-deoxynonulopyranosid)onate obtained by methanolysis of the polysaccharide. On the basis of the results of partial acid hydrolysis, Smith degradation, and computer-assisted 13C-n.m.r. analysis, it was concluded that the capsular polysaccharide has the following structure: (formula see text)
Knirel
Y A
YA
N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow.
Kocharova
N A
NA
Shashkov
A S
AS
Kochetkov
N K
NK
Mamontova
V A
VA
Solovéva
T F
TF
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007709:Klebsiella / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009904:Optical Rotation; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D012703:Serotyping; D013400:Sugar Acids / Q000032:analysis*
D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; C050008:3-deoxyglycero-galacto-nonulosonic acid