Stimulation of the human heat shock protein 70 promoter in vitro by simian virus 40 large T antigen.
Simian virus 40 large tumor (T) antigen stimulates transcription from the SV40 late promoter and some cellular genes. We report here the novel finding that purified T antigen preferentially stimulates transcription from the human heat shock protein 70 promoter in an in vitro transcription system. T antigen is thus capable of stimulating transcription by a process that does not require synthesis of other proteins and that may involve a direct interaction with preexisting cellular factors.
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
I C
IC
Taylor
W
W
Solomon
B M
BM
Weiner
E
E
Paucha
M
M
Bradley
R E
RE
Kingston
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D005796:Genes; D006367:HeLa Cells / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D006360:Heat-Shock Proteins