Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities.
Clones expressing activity against xylan or beta(1-3,1-4)glucan (lichenan) were isolated from a library of Ruminococcus flavefaciens 17 DNA made in bacteriophage lambda EMBL3. Hybridization analyses indicated the recovery of four separate genes encoding xylanases that showed no detectable associated carboxylmethylcellulase activity. One of these genes was associated with clones that also expressed beta(1-3,1-4)glucanase and beta-xylosidase activities.
Nutrition Division, Rowett Research Institute, Bucksburn, Aberdeen, United Kingdom.
H J
HJ
Flint
C A
CA
McPherson
J
J
Bisset
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005786:Gene Expression Regulation; D006026:Glycoside Hydrolases / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010459:Peptococcaceae / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D012417:Rumen / Q000382:microbiology*; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism
D004269:DNA, Bacterial; D014990:Xylans; D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase; C028020:licheninase