[Heparin and its antagonist--a quaternary ammonium salt of oligomer-25-conidine: distribution in subcellular organelles, effect on DNA synthesis in the regenerating rat liver].
Dynamics of distribution in subcellular organelles as well as effects on DNA synthesis of heparin, polycanidine (ChAS oligomer of 25 canidine) and their complexes were studied in regenerating rat liver tissue after partial hepatectomy. Polycanidine and heparin penetrated from circulation into hepatocyte cells, nuclei and mitochondria. After consecutive administration of polyelectrolytes polycanidine increased 2-2.5-fold the amount of heparin entering into cells. Polycanidine formed stable complexes with DNP in nuclei. Heparin and its complexes with polycanidine decreased the DNA synthesis within first day. Heparin appears to be responsible for the inhibitory effect, whereas administration of polycanidine only into animals caused a slight increase in 3H-thymidine incorporation into DNA as compared with controls.
Khatsernova
B Ia
BIa
Silaeva
S A
SA
Golenchenko
V A
VA
Nikolaev
A Ia
AIa
Rozkin
M Ia
MIa
D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D006493:Heparin / Q000493:pharmacokinetics* / Q000494:pharmacology; D006494:Heparin Antagonists / Q000493:pharmacokinetics* / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D015388:Organelles / Q000378:metabolism*; D011108:Polymers / Q000493:pharmacokinetics* / Q000494:pharmacology; D051381:Rats; D013154:Spleen / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D006494:Heparin Antagonists; D011108:Polymers; C039164:conidine; D006493:Heparin; D004247:DNA; D013936:Thymidine
Geparin i ego antagonist--chetvertichnaia ammoniĭnaia sol' oligomera-25-konidina: raspredelenie po subkletochnym organellam, vliianie na sintez DNK v regeneriruiushcheĭ pecheni krys.