UV laser induced RNA-protein crosslinks and RNA chain breaks in tobacco mosaic virus RNA in situ.
The efficiency of RNA-protein crosslink and RNA chain break formation under nanosecond or picosecond UV-laser pulse irradiation of tobacco mosaic virus was determined. It was found that on high-intensity UV-laser irradiation the quantum yields of both reactions increase considerably as compared to the usual (low-intensity) UV-irradiation. The RNA-protein crosslink quantum yield was found to be 1.8 x 10(-5) and 1.2 x 10(-4) and that of RNA chain breaks 1.7 x 10(-4) and 8.9 x 10(-4) for nanosecond and picosecond irradiation, respectively.
E N
EN
Dobrov
Z Kh
ZKh
Arbieva
E K
EK
Timofeeva
R O
RO
Esenaliev
A A
AA
Oraevsky
D N
DN
Nikogosyan
D003432:Cross-Linking Reagents; D007834:Lasers; D011506:Proteins / Q000528:radiation effects*; D012367:RNA, Viral / Q000528:radiation effects*; D014027:Tobacco Mosaic Virus / Q000528:radiation effects*; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D011506:Proteins; D012367:RNA, Viral