MicroELISA method for the determination of thymosin beta 9 discriminating between thymosin beta 9 and the structurally closely related thymosin beta 4.
In order to obtain specific antibodies against thymosin beta 9 showing minimal cross-reactivity with the highly homologous peptide thymosin beta 4, the N-terminal fragment 1-14 of thymosin beta 9 was used for immunization. These antibodies have been tested in a competitive ELISA and show less than 1% cross-reactivity with thymosin beta 4. On the other hand, antibodies raised against the native thymosin beta 9 (1-14) cross-react 35% with thymosin beta 4. Specific antibodies against thymosin beta 9 are important for studying the concentration and localization of thymosin beta 9 in thymus and other bovine tissues because thymosin beta 9 is always accompanied by thymosin beta 4. Using N-terminal fragments of thymosin beta 4-like peptides may be a general approach for obtaining specific antibodies since this part of sequence is less conserved in thymosin beta 4-like peptides.
Abteilung für Physikalische Biochemie, Universität Tübingen, F.R.G.
M
M
Mihelić
H
H
Kalbacher
E
E
Hannappel
W
W
Voelter
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D013947:Thymosin / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000145:classification / Q000276:immunology; D013950:Thymus Gland / Q000032:analysis; D000099201:Thymosin beta(4)
D010446:Peptide Fragments; D013947:Thymosin; D000099201:Thymosin beta(4); C042362:thymosin beta(9)