Restricted degradation of U6 RNA in a nuclear extract of Ehrlich ascites tumor cells.
A new nucleolytic activity that causes restricted digestion of U6 RNA was found in a nuclear extract of Ehrlich ascites tumor cells. This nucleolytic activity specifically degrades U6 RNA in the vicinity of its 3'-end with accumulation of a discrete sized degradation product of RNA of 90-95 nucleotides. Since this degradation product was not digested further by the nuclease under these conditions, this trimming of U6 RNA is supposed to be a biologically meaningful reaction. This nucleolytic activity required Mg2+, and was inhibited by Zn2+ or Ca2+.
Faculty of Pharmaceutical Sciences, University of Tokyo.
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Lee
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Hirai
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Natori
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Sekimizu
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism*; D002478:Cells, Cultured; D008274:Magnesium / Q000494:pharmacology; D012334:RNA, Neoplasm / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D013997:Time Factors; D014529:Uridine / Q000378:metabolism
D012334:RNA, Neoplasm; D012342:RNA, Small Nuclear; D012260:Ribonucleases; D008274:Magnesium; D014529:Uridine