Determination of chlortetracycline residues in tissues using high-performance liquid chromatography with fluorescence detection.
A method is described for the determination of chlortetracycline residues in tissue samples. The samples were extracted into a hydrochloric acid - glycine solution and the extracts concentrated and purified on cyclohexyl-bonded reversed-phase cartridges. Any chlortetracycline present was converted to iso-chlortetracycline at pH 12, which was then separated from interfering compounds on a reversed-phase polymeric column using high-performance liquid chromatography with fluorescence detection. The detection and determination limits of the assay were 20 and 50 ng g-1, respectively, making it suitable for statutory residue testing purposes.
W J
WJ
Blanchflower
R J
RJ
McCracken
D A
DA
Rice
D002751:Chlortetracycline / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D002751:Chlortetracycline