Lymphocyte activation: changes in intracellular adenosine triphosphate and deoxyribonucleic acid synthesis.
Stimulation of human lymphocytes from 20 individuals with phytohaemagglutinin (PHA) for 60 min resulted in a fall in intracellular adenosine triphosphate (ATP) of 35% compared to unstimulated controls of 19% (p less than 0.001). There was a significant (p less than 0.01) correlation (r = 0.57) between ATP decrease at 60 min and deoxyribonucleic acid (DNA) synthesis at 72 h in PHA-stimulated cells. The technique described here is simple, rapid and has possible application in immunodiagnosis and immunologic monitoring.
Department of Organ Transplantation, Faculty of Medicine, Kuwait University.
A G
AG
White
K T
KT
Raju
S
S
Keddie
G M
GM
Abouna
D000255:Adenosine Triphosphate / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D006801:Humans; D008163:Luminescent Measurements; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000378:metabolism; D013997:Time Factors
D010835:Phytohemagglutinins; D000255:Adenosine Triphosphate; D004247:DNA