Effect of sublethal concentration of antibiotics on the adherence of Staphylococcus epidermidis to eukaryotic cells.
Adherence of Staphylococcus epidermidis to pharyngeal epithelial cells as measured by [3H]-leucine-labelled bacteria was found to be a simple, objective and highly reproducible technique. Sublethal concentrations of cephalothin, rifampicin and vancomycin significantly reduced the bacterial adherence to epithelial cells, the effect being most marked with 1/2 MIC. Cloxacillin increased the adherence for one strain while it decreased it for the second. The effect was dose-related and the maximum reduction was observed when bacteria were grown with an antibiotic overnight.
Department of Microbiology, Ministry of Public Health, Safat, Kuwait.
T D
TD
Chugh
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E
Babaa
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G
Burns
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Shuhaiber
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005057:Eukaryotic Cells; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D011817:Rabbits; D012756:Sheep; D013212:Staphylococcus epidermidis / Q000187:drug effects*
D000900:Anti-Bacterial Agents