Sugar composition of the polysaccharide portion of lipopolysaccharides isolated from non-O1 Vibrio cholerae O2 to O41, O44, and O68.
The sugar composition of the polysaccharide portion of lipopolysaccharides (LPS) was determined for 42 serovars of non-O1 Vibrio cholerae, i.e., from serogroups O2 to O41, O44, and O68. On the basis of their compositional sugar pattern, they were classified into 24 chemotypes. 2-Keto-3-deoxyoctonate (KDO) was totally undetectable by the conventional color test (Weissbach's reaction) under the conventional hydrolysis conditions. Instead, a kind of KDO-like substance, which was positive in the reaction but not identical to KDO, was found in serogroup O19. Fructose, a characteristic sugar constituent of O1 V. cholerae LPS, was found in 33 serogroups but was absent from nine serogroups, approximately 20% of the members of this group so far examined.
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama.
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D002241:Carbohydrates / Q000032:analysis; D008070:Lipopolysaccharides / Q000302:isolation & purification; D013058:Mass Spectrometry; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D012703:Serotyping; D013400:Sugar Acids / Q000032:analysis; D014734:Vibrio cholerae / Q000145:classification* / Q000276:immunology
D002241:Carbohydrates; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate