Tse-2: a trans-dominant extinguisher of albumin gene expression in hepatoma hybrid cells.
Serum albumin gene expression is generally extinguished in hepatoma x fibroblast hybrids. To define the genetic basis of this phenomenon, we screened a panel of hepatoma hybrids retaining different fibroblast chromosomes for albumin production by immunofluorescence. We report that albumin extinction in these clones was strictly correlated with the retention of mouse chromosome 1. Furthermore, albumin was systematically reexpressed in chromosome 1 segregants. These data define a tissue-specific extinguisher locus (Tse-2) that affects albumin gene expression in trans. Two other liver genes, those encoding liver alcohol dehydrogenase and liv-10, were coordinately extinguished with albumin in monochromosomal hybrids that specifically retained mouse chromosome 1.
Department of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
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Fournier
D000818:Animals; D002875:Chromosomes; D005786:Gene Expression Regulation; D006822:Hybrid Cells / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000235:genetics; D012709:Serum Albumin / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014960:X Chromosome
D012709:Serum Albumin