Degradation of 2-bromo-, 2-chloro- and 2-fluorobenzoate by Pseudomonas putida CLB 250.
Pseudomonas putida strain CLB 250 (DSM 5232) utilized 2-bromo-, 2-chloro- and 2-fluorobenzoate as sole source of carbon and energy. Degradation is suggested to be initiated by a dioxygenase liberating halide in the first catabolic step. After decarboxylation and rearomatization catechol is produced as a central metabolite which is degraded via the ortho-pathway. After inhibition of ring cleavage activities with 3-chlorocatechol, 2-chlorobenzoate was transformed to catechol in nearly stoichiometric amounts. Other ortho-substituted benzoates like anthranilate and 2-methoxybenzoate seem to be metabolized via the same route.
Institut für Mikrobiologie, Universität Stuttgart, F.R.G.
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D001565:Benzoates / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001970:Bromobenzoates / Q000378:metabolism*; D002396:Catechols / Q000096:biosynthesis; D002723:Chlorobenzoates / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology / Q000378:metabolism*
D001565:Benzoates; D001970:Bromobenzoates; D002396:Catechols; D002723:Chlorobenzoates; C022894:2-fluorobenzoic acid; C061078:2-bromobenzoate; C060415:2-chlorobenzoic acid; C034221:catechol