Geographic variation in Giardia karyotypes.
Chromosomes of 41 stocks of Giardia duodenalis derived from humans and 14 stocks from other animal species were analysed by field inversion gel electrophoresis (FIGE). These stocks have two predominant karyotypes as judged by FIGE which appear to fit a geographic distribution. Under FIGE conditions used to optimize the detection of size variation in Giardia chromosomes, five or six major chromosomes could be identified. Most of the stocks derived from North America have three major chromosomes smaller than 800 kb while most of the Australian stocks have four. A few exceptions, and minor variations, of these karyotypes were observed. It was estimated that not all of the DNA entered the gel, the remainder being trapped conformations or very large chromosomes. Karyotypes of Giardia stocks from different animal hosts and human sources within a geographical region are similar.
J A
JA
Upcroft
P F
PF
Boreham
P
P
Upcroft
D000818:Animals; D002875:Chromosomes; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation; D005872:Giardia / Q000235:genetics*; D006801:Humans; D007621:Karyotyping