Determination of cyclodextrins and branched cyclodextrins by reversed-phase chromatography with pulsed amperometric detection and a membrane reactor.
A high-performance liquid chromatographic method has been developed for the determination of cyclodextrins (CDs) and branched CDs. The method involves their separation on a reversed-phase column using a mixture of water and acetonitrile as an eluant, eluant pH modification with a cation-exchange membrane reactor surrounded by 1.5 M sodium hydroxide solutions, and pulsed amperometric detection with a gold working electrode. The calibration graphs constructed by peak height versus injected amount were linear over the ranges 50-1000 pmol. The detection limits for CDs and branched CDs were about 1-5 pmol at a signal-to-noise ratio of 3. The method was successfully applied to the assay of beta-CD in serum samples.
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
J
J
Haginaka
Y
Y
Nishimura
J
J
Wakai
H
H
Yasuda
K
K
Koizumi
T
T
Nomura
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000097:blood*; D003912:Dextrins / Q000097:blood*; D004563:Electrochemistry; D013213:Starch / Q000097:blood*
D003505:Cyclodextrins; D003912:Dextrins; D013213:Starch