Effect of pyocyanin and 1-hydroxyphenazine on in vivo tracheal mucus velocity.
Products of the bacterium Pseudomonas aeruginosa have been shown to slow the beating of human respiratory tract cilia in vitro. We have tested the effects of two of these compounds, pyocyanin and 1-hydroxyphenazine (given as a bolus dose dissolved in 2 microliters Ringer solution), on tracheal mucus velocity of radiolabeled erythrocytes in anesthetized guinea pigs. 1-Hydroxyphenazine (200 ng) caused a rapid slowing of tracheal mucus velocity (maximum fall 47% at 20 min) with recovery by 1 h. The effect of pyocyanin was slower in onset, 600 ng causing 60% reduction in tracheal mucus velocity at 3 h, and no recovery occurred. A combination of pyocyanin and 1-hydroxyphenazine produced an initial rapid slowing equivalent to the same dose of 1-hydroxyphenazine given alone, but the later slowing attributed to pyocyanin was greater than the same dose administered alone. This study demonstrates one mechanism by which products of P. aeruginosa may facilitate its colonization of the respiratory tract.
Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, United Kingdom.
N C
NC
Munro
A
A
Barker
A
A
Rutman
G
G
Taylor
D
D
Watson
W J
WJ
McDonald-Gibson
R
R
Towart
W A
WA
Taylor
R
R
Wilson
P J
PJ
Cole
D000818:Animals; D006168:Guinea Pigs; D008297:Male; D009079:Mucociliary Clearance / Q000187:drug effects*; D010619:Phenazines / Q000494:pharmacology*; D011710:Pyocyanine / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects* / Q000502:physiology
D010619:Phenazines; C050093:1-hydroxyphenazine; D011710:Pyocyanine