Application of an ultrahigh-resolution scanning electron microscope (UHS-T1) to biological specimens.
In 1985 we developed an ultrahigh-resolution scanning electron microscope with a resolution of 0.5 nm. It is equipped with a field emission gun and an objective lens with a very short focal length. In this study we report a survey of some different preparation techniques and biological specimens using the new scanning electron microscope. Intracellular structures such as cell organelles were observed surprisingly sharper than those observed by ordinary scanning electron microscopes. However, at magnifications over 250,000 x, platinum particles could be discerned as scattered pebbles on the surface of all structures in coated materials. Using an uncoated but conductively stained specimen, we successfully observed ribosomes on a rough endoplasmic reticulum at a direct magnification of 1 million. In these images some protrusions were recognized on the ribosomes. Ferritin and immunoglobulin G were used as samples of biological macromolecules. These samples were observed without metal coating and conductive staining. The ferritin particles appeared as rounded bodies without any substructure on the surface and immunoglobulin G as complexes of three-unit bodies. In the latter the central body might correspond to the Fc fragment and two side ones to Fab fragments. We assume that ultrahigh-resolution scanning electron microscopy is an effective means for observation of the cell fine structures and biological macromolecules. It will open a new research field in biomedicine.
Tanaka
K
K
Department of Anatomy, Tottori University School of Medicine, Yonago, Japan.
Mitsushima
A
A
Kashima
Y
Y
Nakadera
T
T
Osatake
H
H
D000818:Animals; D001435:Bacteriophages / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D005293:Ferritins / Q000648:ultrastructure; D005347:Fibroblasts / Q000648:ultrastructure; D007074:Immunoglobulin G / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009474:Neurons / Q000648:ultrastructure; D015388:Organelles / Q000648:ultrastructure; D010902:Pituitary Gland / Q000648:ultrastructure; D051381:Rats; D013116:Spinal Cord / Q000648:ultrastructure
D007074:Immunoglobulin G; D005293:Ferritins