Preparation of [35S]sulfobromophthalein of high specific activity.
Study of the hepatocyte transport mechanism of organic anions such as bilirubin and sulfobromophthalein has been limited by the relatively low specific activities of these ligands. [3H]Bilirubin and [35S]sulfobromophthalein have been available with specific activities of only approximately 100 mCi/mmol. We now report a relatively simple procedure to prepare [35S]sulfobromophthalein at a specific activity of approximately 3000 mCi/mmol. This compound is radiochemically pure and serves as a tracer for authentic sulfobromophthalein as judged by chromatography, hepatocyte uptake, metabolism, and biliary excretion. Use of this material as a photoaffinity probe and as a transported ligand may permit dissection and understanding of its transport mechanism.
Liver Research Center, Albert Einstein College of Medicine, Bronx, New York.
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Nilprabhassorn
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Wolkoff
D000818:Animals; D001646:Bile / Q000378:metabolism; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D066298:In Vitro Techniques; D007553:Isotope Labeling; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D051381:Rats; D013448:Sulfobromophthalein / Q000138:chemical synthesis* / Q000302:isolation & purification; D013462:Sulfur Radioisotopes; D013464:Sulfuric Acids / Q000302:isolation & purification
D013462:Sulfur Radioisotopes; D013464:Sulfuric Acids; D013448:Sulfobromophthalein