[Invasive and non-invasive studies of the protective action of a silicon-containing cream and its excipient in skin irritation induced by sodium laurylsulfate].
The purpose of our study was to evaluate the protective effect of a new silicone-containing barrier-cream (Anthydro) and its vehicle (Anthydro without silicone) in the prevention of cutaneous irritation by detergents. We therefore planned a study in several stages, using an anionic surfactant well known for its irritant properties: sodium laurylsulphate (SLS) in aqueous solution. In a first series of experiments, the protective effect of the Anthydro cream against SLS was studied by invasive methods on guinea-pigs in order to determine histologically the protective effect of the cream when a 10 p. 100 SLS solution was applied on the skin under occlusion during 24 hours (Square chambers, Van der Bend). Typical and reproducible lesions were apparent, and the protected sites were compared with the unprotected sites. In parallel, we used non-invasive methods (conductivity, transepidermal water loss and cutaneous blood flow) to determine in humans the protective effect of Anthydro cream in comparison with unprotected sites after application during 24 hours of patches soaked with a 5 p. 100 SLS solution on the forearms of 13 adult and healthy volunteers (Silver patch tests, Van der Bend). In both experiments the Anthydro cream was effective in reducing the SLS-induced cutaneous irritation. In a second series of experiments, the Anthydro barrier-cream was compared with its "base" (Anthydro without silicone) in terms of effectiveness, following the same experimental procedure (invasive on guinea-pigs, and non-invasive on humans). The base was shown to be effective in protecting against irritation. However, the histological lesions were less intense when the skin was protected by Anthydro than by its vehicle. Concerning the non-invasive methods in humans, no significant statistical differences appeared in the measurement of various parameters between the sites protected by Anthydro and the sites protected by the vehicle. These results lead us to suggest the existence of an essentially mechanical protective effect in which the silicone plays a very small part in terms of effectiveness. These experimental results necessitates further investigations to be extrapolated to occupational conditions without tests performed in industries and well-conducted epidemiological investigations.
Département de Médecine du Travail, Université Catholique de Louvain.
H
H
Nouaigui
J L
JL
Antoine
M L
ML
Masmoudi
D J
DJ
Van Neste
J M
JM
Lachapelle
D000818:Animals; D003877:Dermatitis, Contact / Q000188:drug therapy* / Q000209:etiology; D004129:Dimethylpolysiloxanes / Q000494:pharmacology*; D004338:Drug Combinations / Q000494:pharmacology; D004341:Drug Evaluation; D004655:Emulsions; D005079:Excipients; D006168:Guinea Pigs; D006801:Humans; D009824:Ointments; D010328:Patch Tests; D012827:Silicone Oils; D012828:Silicones / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000009:adverse effects*; D013844:Thiazoles / Q000494:pharmacology*
C061177:Anthydro; D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; D004655:Emulsions; D005079:Excipients; D009824:Ointments; D012827:Silicone Oils; D012828:Silicones; D013844:Thiazoles; D012967:Sodium Dodecyl Sulfate
Etudes invasive et non invasive du pouvoir protecteur d'une crème siliconée et de son excipient vis-à-vis de l'irritation cutanée induite par le laurylsulfate de sodium.