Some characteristics of a retrovirus isolated from transformed bovine cells.
A novel retrovirus has been isolated from bovine cells transformed after cocultivation with leukocytes from an animal suffering from sheep-associated bovine malignant catarrhal fever. Morphologically and in its morphogenesis the virus resembles the type D Mason-Pfizer monkey virus (MPMV) as well as the ovine jaagsiekte retrovirus (JSRV). It is also serologically related to these two viruses, whereas a relationship to other known bovine retroviruses was not detected. However, its density of 1.16 g/ml in sucrose and the preference of its reverse transcriptase for manganese ions are closer to the characteristics of type C retroviruses. Unsuccessful attempts at transmission suggest that the virus may be defective in some function affecting its infectivity.
Veterinary Research Institute, P.O. Onderstepoort, South Africa.
D F
DF
York
A
A
Williamson
B J
BJ
Barnard
D W
DW
Verwoerd
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D002417:Cattle / Q000382:microbiology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D007962:Leukocytes / Q000382:microbiology; D051379:Mice; D008819:Mice, Nude; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000382:microbiology / Q000473:pathology; D012190:Retroviridae / Q000276:immunology / Q000302:isolation & purification*; D014764:Viral Proteins / Q000032:analysis
D000914:Antibodies, Viral; D014764:Viral Proteins