Protein adsorption to hydroxyapatite and to calcium fluoride in vitro and amino acid analyses of pellicle formed on normal enamel and on calcium-fluoride-covered enamel in vivo.
Fluoride treatment of enamel has been reported to result in the formation of a layer of a CaF2-like material on the enamel surface. Protein adsorption to enamel is a specific process dependent on the nature of the surface, and little is known about protein adsorption to CaF2. Albumin and lysozyme were adsorbed to hydroxyapatite (HA) and CaF2 powder in vitro, and protein adsorption patterns constructed. In vivo pellicle was collected from three volunteers from fluoride-treated enamel and from normal enamel, and the amino acid compositions analyzed separately. The results showed that CaF2 took up small amounts of proteins as compared with HA. When the CaF2 was pretreated with a phosphate buffer, pH 6.8, the protein adsorption increased markedly. The amino acid analyses showed no major differences in the amino acid compositions between pellicle collected from CaF2-covered enamel and pellicle collected from normal enamel. This lack of difference is presumably due to the adsorption of phosphate ions to the CaF2 crystals and hence changed surface properties.
Dental Faculty, University of Oslo, Norway.
M
M
Rykke
T
T
Sønju
K
K
Skjørland
G
G
Rølla
D000327:Adsorption; D000418:Albumins / Q000493:pharmacokinetics; D000596:Amino Acids / Q000032:analysis*; D002124:Calcium Fluoride / Q000493:pharmacokinetics; D003741:Dental Deposits / Q000032:analysis* / Q000378:metabolism; D003743:Dental Enamel / Q000032:analysis* / Q000378:metabolism; D044622:Dental Pellicle; D004577:Electron Probe Microanalysis; D006801:Humans; D006882:Hydroxyapatites; D066298:In Vitro Techniques; D009113:Muramidase / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet
D000418:Albumins; D000596:Amino Acids; D006882:Hydroxyapatites; D009113:Muramidase; D002124:Calcium Fluoride