[A rapid test of urine microflora sensitivity to antibacterial drugs in the treatment of children with nonspecific inflammatory diseases of the kidney and urinary tract].
The authors describe their own method for rapid determination of urine microflora sensitivity to antibacterial drugs. The method is based on the capacity of the microorganisms (as a totality of particles with the refractive index different from that of the medium) of increasing the optical density of the medium under the conditions of unlimited resources of the nutrition and space at the expense of reproduction in a liquid culture medium. The lack of the optical density increase in the urine sample after addition to it of a certain amount of antibacterial substances evidences the death of the population of the microorganisms and of its sensitivity to the antibacterial drug under study. The method proposed by the authors was compared to those widely used in clinical practice. With special reference to a concrete patient, the results obtained with the authors' method turned out to correlate with those derived with the use of the conventional methods for urine microflora sensitivity to antibacterial drugs (the disc method and the triphenyltetrazolium chloride test). That the final result of the investigation can be obtained after 2--6 hours and low labour intensivity of the method permit the institution of adequate antibiotic therapy within the first day since the patient's admission to the hospital.
Rodoman
V E
VE
Laberko
L A
LA
Korotaev
A L
AL
Zarma
A A
AA
D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D002648:Child; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000188:drug therapy* / Q000382:microbiology; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods*; D014552:Urinary Tract Infections / Q000188:drug therapy* / Q000382:microbiology; D014556:Urine / Q000382:microbiology*
D000900:Anti-Bacterial Agents
Uskorennoe opredelenie chuvstvitel'nosti mikroflory mochi k antibakterial'nym preparatam v lechenii deteĭ s nespetsificheskimi vospalitel'nymi zabolevaniiami pochek i mochevyvodiashchikh puteĭ.