Chlamydia trachomatis oculogenital infection in the subcutaneous autotransplant model of conjunctiva, salpinx and endometrium.
The subcutaneous pocket model of salpingeal, endometrial, and conjunctival autografts for studying Chlamydia trachomatis infection in monkeys is described. Portions of the salpinx that were transplanted included fimbria, ampulla, and isthmus. The model is an extension of the original model which consists of either salpingeal fimbria or conjunctive autografts. Transplantation of the ampulla portion of the Fallopian tube enabled us to increase the number of pockets or test sites. Salpingeal and conjunctival autografts could be established during a single surgery. In addition, it is possible to autotransplant endometrium and provoke endometritis. The autografts were shown to be susceptible to C. trachomatis infection. Preliminary rechallenge experiments showed infection of the subcutaneous transplants may induce immunity, indicating the model may be used for immunity and vaccine studies. Simultaneous transplantation of different parts of the oviduct, endometrium, and conjunctive should expand the usefulness of the subcutaneous model in other studies on mixed infections or immune responses to infection.
Department of Obstetrics and Gynecology, University of Washington, Seattle.
D L
DL
Patton
C C
CC
Kuo
R M
RM
Brenner
D000818:Animals; D002690:Chlamydia Infections / Q000473:pathology*; D002692:Chlamydia trachomatis; D003228:Conjunctiva / Q000637:transplantation*; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004717:Endometrium / Q000637:transplantation*; D005187:Fallopian Tubes / Q000637:transplantation*; D005260:Female; D008251:Macaca; D014182:Transplantation, Autologous