The primary structure of a protein carboxyl methyltransferase from bovine brain that selectively methylates L-isoaspartyl sites.
Protein L-isoaspartyl methyltransferase (PIMT) transfers the methyl group of S-adenosyl-L-methionine to free alpha-carboxyl groups of atypical L-isoaspartyl residues in proteins. The complete primary structure of the type I isoform of bovine brain PIMT was determined by sequence analysis of peptides generated by endoprotease Lys-C, trypsin, cyanogen bromide, and endoprotease Asp-N digests. The correct composition of every peptide was verified by fast atom bombardment mass spectrometry. The efficiency of sequencing by tandem mass spectrometry was examined for several peptides by comparing its speed and accuracy with automated Edman degradation. Tandem mass spectrometry was used to determine the structure of the NH2-terminal blocked peptide derived from a hydroxylamine cleavage. PIMT is 226 residues with Mr = 24,500 and contains acetyl alanine as the amino-terminal residue. The partial sequence (141 residues from 8 tryptic peptides) of a homologous human red cell PIMT (Gilbert, J. M., Fowler, A., Bleibaum, J., and Clarke, S. (1988) Biochemistry 27, 5227-5233) shows a 97% identity with the corresponding peptides of the bovine brain enzyme. The complete brain enzyme sequence reported here bears no significant homology to any other known class of methyltransferase including those which methylate the side chain gamma-carboxyl group of receptor proteins involved in bacterial chemotaxis.
Department of Protein Chemistry, Genentech, Inc., South San Francisco, California 94080.
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Henzel
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Stults
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Hsu
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Aswad
D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007536:Isomerism; D008745:Methylation; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D011496:Protein Methyltransferases / Q000378:metabolism*; D011497:Protein O-Methyltransferase / Q000378:metabolism*; D013379:Substrate Specificity; D014357:Trypsin
D010446:Peptide Fragments; D001224:Aspartic Acid; D011496:Protein Methyltransferases; D011497:Protein O-Methyltransferase; D014357:Trypsin