Identification of C-3 on polyacrylamide gel by pre-staining the normal human serum with Remazol Brilliant Blue and raising monospecific anti C-3 antibody.
Based on our earlier work we hereby describe pre-staining of normal human serum with Remazol Brilliant Blue (RBB) to precisely locate the third component of complement system (C3,B1C) on polyacrylamide gels. Testing the dye-protein complex eluted from different immunoreactive post-transferrin (origin to transferrin) components against the standards anti-C3 antibody in various gel precipitation techniques, revealed that the prominent band just posterior to the centrally placed transferrin is of complement. Subsequent to its identity, we also evolved a method to raise an anti-C3 antibody. The resulting antibody was found to be monospecific when assessed in different gel precipitation procedures.
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D000880:Anthraquinones; D000906:Antibodies; D004396:Coloring Agents; D003176:Complement C3 / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans
D000880:Anthraquinones; D000906:Antibodies; D004396:Coloring Agents; D003176:Complement C3; C024758:Remazol Brilliant Blue R