A scanning electron microscope study of fetal eyelid closure accelerated by cortisone in SWV/Bc mice.
Eyelid closure occurs earlier in SWV/Bc and CBA/J mouse fetuses whose mothers were treated with cortisone on day 14 of gestation than it does in fetuses from untreated mothers. Similar treatment prevents the open-eyes defect of lidgap-Miller mutant mice, but examination by scanning electron microscope (SEM) has shown differences in the periderm of the closing eyelids of the cortisone-treated mutant compared with those of untreated genetically normal fetuses. (Untreated mutant eyelids remain wide open and very abnormal). The present study has examined at the SEM level the accelerated eyelid closure of cortisone-treated normal strain, SWV/Bc, fetuses to investigate whether the differences from normal in the eyelids of treated lidgap-Miller fetuses are part of the mechanism of the cortisone "cure." At the SEM level, cortisone-accelerated eyelid closure of SWV/Bc fetuses is indistinguishable from that in untreated fetuses. This suggests that the early eyelid closure induced by cortisone in normal strain fetuses represents acceleration of the normal coordinated sequence of events that leads to closure, rather than an abnormality that fortuitously leads to closure. The data also indicate that the cellular abnormalities seen previously in treated lidgap-Miller mutant fetuses are a combination of 1) abnormalities due to the mutation that are not completely reversed by cortisone and 2) normal developmental stages that have become concurrent with the cortisone-induced late closure in lidgap-Miller mutant fetuses.
Juriloff
D M
DM
Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
Harris
M J
MJ
D000818:Animals; D003348:Cortisone / Q000494:pharmacology*; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005143:Eyelids / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure; D005260:Female; D005865:Gestational Age; D008431:Maternal-Fetal Exchange; D051379:Mice; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning; D011247:Pregnancy; D013997:Time Factors
D003348:Cortisone