The cholinergic system in developing cerebellum: comparative study of normal, hypothyroid and underfed rats.
To overcome the deficiencies of previous findings, the activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AchE) were studied at very short age intervals to allow a more precise definition of the shape and timing of their developmental curves in normal, hypothyroid and underfed rats. In addition, AchE expression in developing cerebellum was studied histochemically in these three neurological models. When compared with structural findings in the literature, the results provide the following information on the normal and abnormal developing cholinergic system, related or not to cerebellar neurotransmission (1) AchE activity, unlike ChAT, can be considered as a good marker of the developing cholinergic archicerebellum. (2) ChAT and AchE are transiently expressed together in functionally noncholinergic Purkinje cells. In contrast with most regions of the central nervous system, the high ratio of ChAT to AchE activities in the early stage of cerebellar development suggests an enhanced synthesis of acetylcholine (Ach). The level of ChAT activity correlates with Purkinje cell size, supporting the concept of a neurotrophic role of Ach in early maturing macroneurons. (3) The archicerebellar cholinergic network appears to be relatively well preserved from undernutrition and, to an even greater extent, from hypothyroidism, compared to other systems of neurotransmission formed later and more widely distributed throughout the cerebellum. The presynaptic compartment seems to be more affected than the postsynaptic compartment. (4) In disagreement with some data in the literature, the abnormalities induced by both abnormal thyroidal and nutritional states were found to be irreversible.
UA 1197 du CNRS, Neurobiologie endocrinologique, Université des Sciences et Techniques du Languedo, Montpellier, France.
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Clos
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Ghandour
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Eberhart
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Vincendon
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Gombos
D000110:Acetylcholinesterase / Q000378:metabolism*; D000375:Aging; D000818:Animals; D002531:Cerebellum / Q000201:enzymology / Q000254:growth & development*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D006651:Histocytochemistry; D007037:Hypothyroidism / Q000201:enzymology*; D009748:Nutrition Disorders / Q000201:enzymology*; D011689:Purkinje Cells / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase