Corticosteroid-induced alkaline phosphatase isoenzyme in the diagnosis of canine hypercorticism.
Corticosteroid-induced isoenzyme of alkaline phosphatase (AP) can easily be demonstrated in canine plasma as a routine procedure because of its greater heat stability at 65 degrees C in comparison with that of other AP-isoenzymes. In this study the accuracy of this test for the diagnosis of hypercorticism was investigated. The AP-65 degrees C test had its highest efficiency when applied to plasma AP levels exceeding 150 units/litre. In a group of 146 dogs, clinically suspected of having hyperadrenocorticism, the test had a sensitivity of 0.92 and a positive predictive value for a positive test result of 0.89. Its lack of specificity (0.44) makes it unsuitable as a diagnostic test. The main application of AP-65 degrees C is in detecting hypercorticism in dogs by routine laboratory measurements, as was demonstrated in 711 dogs, in which a positive predictive value for the presence of hypercorticism of 0.89 was found.
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, State University of Utrecht, The Netherlands.
E
E
Teske
J
J
Rothuizen
J J
JJ
de Bruijne
A
A
Rijnberk
D000308:Adrenocortical Hyperfunction / Q000175:diagnosis / Q000201:enzymology / Q000662:veterinary*; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D004796:Clinical Enzyme Tests / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis* / Q000201:enzymology; D004285:Dogs; D007527:Isoenzymes / Q000097:blood*; D011237:Predictive Value of Tests
D007527:Isoenzymes; D000469:Alkaline Phosphatase