Effects of zinc on macromolecular synthesis and nuclear division during the yeast to mycelium transition in Sporothrix schenckii.
Zinc ions (10 mM) have been reported previously to inhibit the yeast to mycelium transition in Sporothrix schenckii. Yeast cells of this fungus were harvested, selected by filtration and allowed to form germ tubes in a basal medium with glucose in the presence of 10 mM zinc and the effects of this ion on protein, RNA and DNA synthesis and nuclear division recorded. All of these processes were affected by the addition of 10 mM zinc to the medium. Nevertheless, the inhibition of protein synthesis was observed earlier than that of RNA or DNA synthesis and was of a greater magnitude than that observed for both of these processes. Protein synthesis was inhibited within the first hour after inoculation, at which time this process begins in the control cells. RNA synthesis was inhibited during the 3 to 6 h interval after inoculation, that is, 3 h after the start of this process in the control cells. After 9 h of incubation, the inhibition of protein synthesis had reached its maximum at 70%, while that of RNA synthesis was only 52%. DNA synthesis was slightly inhibited, with maximum inhibition being observed 9 h after inoculation. Nuclear division in cells forming germ tubes in the presence of 10 mM zinc took place with a 3 h delay in relation to the control cells. These observations suggest that the inhibition of protein synthesis might be the most important mechanism by which zinc inhibits the yeast to mycelium transition in S. schenckii.
Department of Microbiology and Medical Zoology, University of Puerto Rico, San Juan 00936.
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Rodríguez-del Valle
D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology; D003470:Culture Media; D004271:DNA, Fungal / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis*; D006801:Humans; D012331:RNA, Fungal / Q000096:biosynthesis*; D013175:Sporothrix / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D015032:Zinc / Q000494:pharmacology*
D003470:Culture Media; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; D015032:Zinc