Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum.
Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two other plasmids, pGK12 and pSA3, were transformed into L. plantarum, and the stability of each plasmid was evaluated.
Department of Agricultural Biochemistry and Nutrition, University of Newcastle upon Tyne, England.
E E
EE
Bates
H J
HJ
Gilbert
G P
GP
Hazlewood
J
J
Huckle
J I
JI
Laurie
S P
SP
Mann
D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D003013:Clostridium / Q000201:enzymology / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D007778:Lactobacillus / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping; D012820:Silage; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D002480:Cellulase