Electron microscopy of gold-labeled human and equine chromosomes.
We present an immunochemical technique for the detection of 5-bromo-2'-deoxyuridine (BrdU) incorporated discontinuously into the chromosomal DNA. A monoclonal anti-BrdU antibody and a protein A-gold complex were used to produce chromosome banding of human and equine chromosomes, specific for electron microscopy (EM). Well-defined bands, symmetry of sister chromatids, concordance between homologues, and band patterns similar to those observed by light microscopy facilitate chromosome identification and karyotyping. From prophase to late metaphase, chromosomes condense and bands appear to fuse. The fusion appears to be owing to chromatin reorganization. Our results underline the value of using immunogold reagents, which are ideal probes for antigen localization on chromosomes.
Département d'Anatomie, Faculté de Médecine, Université de Montréal, Québec, Canada.
P E
PE
Messier
R
R
Drouin
C L
CL
Richer
D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000378:metabolism; D002871:Chromosome Banding / Q000379:methods; D002875:Chromosomes / Q000378:metabolism* / Q000648:ultrastructure; D002877:Chromosomes, Human / Q000378:metabolism* / Q000648:ultrastructure; D006736:Horses / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine