gamma-Aminobutyric acid uptake by rat kidney brush-border membrane vesicles.
Brush-border membrane vesicles (BBMV) from rat kidney cortex possessed two uptake systems for gamma-aminobutyric acid (GABA), a high affinity system (Km = 10.9 microM) and a low affinity system (Km = 1203 microM). Both uptake systems were inhibited by p-hydroxymercuribenzoic acid and ouabain, and by the action of neuraminidase, whereas the GABA analogs nipecotic acid, beta-alanine, 2,4-diaminobutyric acid and 4,5,6,7-tetrahydroisoxazolo-[4,5 c]-pyridin-3-ol had no effect on the GABA uptake activity. The BBMV uptake systems were clearly different from the GABA transport systems present in brain tissue.
Sidhu
H S
HS
Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
Wood
J D
JD
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D009557:Nipecotic Acids / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D009557:Nipecotic Acids; D005680:gamma-Aminobutyric Acid; D009439:Neuraminidase