Inhibition of ornithine carbamoyltransferase from Pseudomonas syringae pv. syringae W50 by phaseolotoxin.
In contrast to the producer of phaseolotoxin and Orn-P(O)(NH2)-NH-SO3H (PNSOrn), Pseudomonas syringae pv. phaseolicola, which possesses a sensitive and an insensitive type of ornithine carbamoyltransferase (OCT, E.C. 2.1.3.3.), in Pseudomonas syringae pv. syringae W50, an organism which produce neither phaseolotoxin nor PNSOrn, only one type of OCT could be detected. This enzyme is highly sensitive to phaseolotoxin. This result supports our hypothesis that the existence of an insensitive ornithine carbamoyltransferase is an important prerequisite for the synthesis of phaseolotoxin in P. syringae pv. phaseolicola and that this enzyme does not occur generally in P. syringae spec.
Sektion Biologie der Friedrich-Schiller-Universität Jena, WB Mikrobielle Biochemie.
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Jahn
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Sauerstein
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Reuter
D004351:Drug Resistance; D005098:Exotoxins / Q000494:pharmacology*; D009952:Ornithine / Q000031:analogs & derivatives; D009954:Ornithine Carbamoyltransferase / Q000037:antagonists & inhibitors*; D011549:Pseudomonas / Q000201:enzymology*
D005098:Exotoxins; C021234:phaseolotoxin; D009952:Ornithine; D009954:Ornithine Carbamoyltransferase