A histochemical study of the distribution of aldehyde dehydrogenase activity in brain structures of rats with genetically different alcohol-related behaviour.
Brain samples from rats genetically selected for high or low voluntary alcohol intake (AA and ANA strains) or for differences in alcohol-induced motor incoordination (AT and ANT strains) were analyzed by histochemistry for aldehyde dehydrogenase (EC 1.2.1.3; ALDH) activity in various CNS structures. All strains exhibited the highest ALDH activities in neurons of the mesencephalic tract of trigeminal nerve nucleus and in spinal cord motoneurons, while the lowest activities were observed in the somatosensory cortex. Although the general distribution pattern of ALDH activity was similar in the genetically selected strains, some potentially important differences were observed. AA rats with high voluntary alcohol consumption had lower ALDH activity (with acetaldehyde as substrate) in the neuropil of the olfactory tubercle but higher activity (with benzaldehyde as substrate) in the spinal cord motoneurons, Purkinje cells and capillary endothelium of the cerebellum as compared to the corresponding structures from the alcohol avoiding ANA rats. Alcohol-resistant AT rats had higher ALDH activity, with benzaldehyde, in most CNS structures than did the alcohol-sensitive ANT's, significantly so in the lamina II of the somatosensory cortex and the neurons of the lateral hypothalamic area. This relationship was also found with acetaldehyde as substrate in the neurons of the hypothalamic arcuate nuclei and in cerebellar capillaries, but the ANT's had the higher activity in the neurons of the cerebral cortex V lamina. We suggest that some of the differences observed may relate to the differences between the rat strains with respect to voluntary alcohol intake and alcohol-induced motor incoordination.
Institute of Biochemistry, Byelorussian SSR Academy of Sciences, Grodno.
S
S
Zimatkin
K O
KO
Lindros
D000428:Alcohol Drinking; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D004730:Endothelium, Vascular / Q000201:enzymology; D006651:Histocytochemistry; D008297:Male; D009474:Neurons / Q000201:enzymology; D051381:Rats / Q000235:genetics*; D014018:Tissue Distribution
D000444:Aldehyde Dehydrogenase