Production and characterization of four monoclonal antibodies specific for human interferon-alpha-1 and -alpha-2.
Four monoclonal antibodies against HuIFN-alpha-1 and -alpha-2 were produced by the lymphocyte hybridoma technique. The screening for monoclonal anti-IFN antibodies was performed by two different procedures permitting the detection of neutralizing and non-neutralizing antibodies. The first antibody screening in the supernatants of hybridoma clones was carried out by an immunodot ELISA using a novel chromogen, 2-bromo-1-naphthol, for the immunoperoxidase reaction. The second screening was performed with a solid-phase bioassay. The monoclonal antibodies IIG11 and IVH2 react specifically with HuIFN-alpha-1 independently of its origin, natural or recombinant. The mAb IIG11 recognizes also an epitope on a 27 kDa interferon, probably HuIFN-alpha-8. The mAb IIG7 binds to HuIFN-alpha-2 only, whereas the mAb IIB2 binds to HuIFN-alpha-2 and also to the 27 kDa interferon and to a 25 kDa interferon, not yet further characterized. These antibodies can be used successfully for immunopurification as well as for quantification of the corresponding interferons.
Central Institute of Molecular Biology, Academy of Sciences of GDR, Berlin-Buch.
F
F
Noll
W
W
Handschack
E
E
Eichmann
M
M
Gaestel
F
F
Schneider
R
R
Kuhl
K
K
Löster
D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000937:Antigen-Antibody Reactions; D001681:Biological Assay; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007370:Interferon Type I / Q000032:analysis* / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D008970:Molecular Weight; D009500:Neutralization Tests; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D011994:Recombinant Proteins