The accumulation of malonyldialdehyde, an end product of membrane lipid peroxidation, can cause potassium leak in normal and sickle red blood cells.
This study has examined the effect of malonyldialdehyde (MDA), an end product of lipid peroxidation, on the K+ leak in normal (AA) and sickle (SS) red blood cells (RBCs). In vitro MDA accumulation in human RBCs was accomplished by treating them with exogenous standard MDA. MDA accumulation assessed by the thiobarbituric acid reactivity of in vitro MDA-treated RBCs was comparable to the RBCs in hemolytic anemias. There was a significant K+ leak in AA RBCs after in vitro treatment with MDA. The effect of MDA on the K+ leak was greater in SS RBCs. The increase in cellular K+ leak was significantly positively correlated with the extent of MDA accumulation as assessed by thiobarbituric acid reactivity.
Department of Pediatrics, Louisiana State University School of Medicine, Shreveport 71130.
S K
SK
Jain
J D
JD
Ross
G J
GJ
Levy
R L
RL
Little
J
J
Duett
D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects*; D004913:Erythrocytes, Abnormal / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015227:Lipid Peroxidation / Q000187:drug effects*; D008314:Malonates / Q000494:pharmacology*; D008315:Malondialdehyde / Q000097:blood / Q000494:pharmacology*; D008563:Membrane Lipids / Q000097:blood*; D008875:Middle Aged; D009994:Osmolar Concentration; D011188:Potassium / Q000097:blood*
D008314:Malonates; D008563:Membrane Lipids; D008315:Malondialdehyde; D011188:Potassium