Nucleotide sequence and complementation studies of the gene 10 region of bacteriophage T3.
The nucleotide sequence of bacteriophage T3 gene 10 and surrounding regulatory elements has been determined and compared to the analogous region of bacteriophage T7. T3 genes 9, 10 and 11 have been shown to complement T7 mutants. The DNA sequences of T3 and T7 gene 10A are homologous, as are the amino acid sequences of the respective products. The translational shift to the -1 frame is predicted to occur at the same position in gene 10 of T3 and T7, though different nucleotide sequences are probably responsible. The resulting gp10B products have completely different C termini.
Department of Microbiology, University of Texas, Austin 78712.
J P
JP
Condreay
S E
SE
Wright
I J
IJ
Molineux
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases; D005814:Genes, Viral; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013604:T-Phages; D014158:Transcription, Genetic
D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases