Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.
A general and efficient transfection procedure, based on compacted lipopolyamine-coated plasmids, has been developed. The active species is obtained by simple addition of excess synthetic lipospermine solution to the DNA and binds within minutes to the cell membrane. This technique has been developed on endocrine cells of the intermediate lobe of the pituitary as a general tool for physiological work on primary cells; it is not toxic and does not interfere with physiological regulations in melanotrope cells. A variety of eukaryotic cell cultures also have been transfected with success for transient and stable expression.
Laboratoire de Chimie organique physique, Unité Associee 422 du Centre National de la Recherche Scientifique, Insitut Le Bel, Strasbourg, France.
J P
JP
Behr
B
B
Demeneix
J P
JP
Loeffler
J
J
Perez-Mutul
D000818:Animals; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005998:Glycine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D007700:Kinetics; D008081:Liposomes; D010714:Phosphatidylethanolamines / Q000138:chemical synthesis; D010902:Pituitary Gland / Q000378:metabolism; D010957:Plasmids; D013096:Spermine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D013552:Swine; D014158:Transcription, Genetic; D014162:Transfection
D008081:Liposomes; D010714:Phosphatidylethanolamines; C061134:dioctadecylamidoglycylspermine; C061135:1,2-dipalmitoylphosphatidylethanolamidospermine; D013096:Spermine; D015500:Chloramphenicol O-Acetyltransferase; D005998:Glycine