Modulations in the biotransformation of tobacco extract and N'-nitrosonornicotine under differential dietary protein status.
The modulation of the phase I and phase II biotransformation enzymes upon treatment with tobacco extract (TE) and N'-nitrosonornicotine (NNN) was investigated using male Sprague-Dawley rats fed differential protein diets. It was observed that the animals fed a low protein diet showed an overall decrease in the basal levels of hepatic and pulmonary phase I and II enzymes. TE and NNN significantly decreased the detoxifying system in the low-protein-fed animals. Animals fed 20% protein, however, showed significant increases in glutathione and glutathione S-transferase upon treatment. Furthermore, TE and NNN treatment brought about a significant depletion in the hepatic pool of vitamin A with a concomitant increase in the vitamin C levels.
Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
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Ammigan
U J
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Nair
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Amonkar
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Bhide
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001711:Biotransformation; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003573:Cytochrome b Group / Q000378:metabolism; D015786:Cytochromes b5; D004032:Diet; D004044:Dietary Proteins / Q000378:metabolism / Q000494:pharmacology*; D007928:Lethal Dose 50; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009602:Nitrosamines / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*; D010947:Plants, Toxic; D051381:Rats; D011919:Rats, Inbred Strains; D014026:Nicotiana; D014801:Vitamin A / Q000378:metabolism
D002273:Carcinogens; D003573:Cytochrome b Group; D004044:Dietary Proteins; D009602:Nitrosamines; D010936:Plant Extracts; D014801:Vitamin A; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D001205:Ascorbic Acid; C008655:N'-nitrosonornicotine