Coupled-column chromatography on immobilized protein phases for direct separation and determination of drug enantiomers in plasma.
Columns packed with immobilized alpha 1-acid glycoprotein and albumin were used in coupled-column chromatography to increase their utility for determining low concentrations of enantiomers in biological samples. The two enantiomers eluted from the protein columns were trapped and compressed on two separate columns, packed with hydrophobic stationary phase, and subsequently transferred to a fourth column for final separation. The overall effect was an increase in efficiency and selectivity. Examples are given of separations of the enatiomers of terbutaline, metoprolol, oxazepam and bupivacaine in plasma. For quantitative determination a single calibration can be used for both enantiomers.
Technical Analytical Chemistry, University of Lund, Sweden.
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Walhagen
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LE
Edholm
D055598:Chemical Phenomena; D002621:Chemistry; D002853:Chromatography, Liquid; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood*; D011506:Proteins; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations; D011506:Proteins