Large-scale synthesis of the mRNA analogue C17AUGA4C17.
The large-scale synthesis of the mRNA analogue C17AUGA4C17 is described. This molecule is 41 bases in length and contains three unique codons in its central region. tRNA binding studies at 6 mM Mg2+ in the absence and presence of the labeled heteropolymer show the mutual stimulation of tRNA and mRNA binding and demonstrate that this mRNA provides a simple and suitable system for the analysis of the reactions of the ribosomal elongation cycle.
Gnirke
A
A
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin-Dahlem.
Nierhaus
K H
KH
D001483:Base Sequence; D001665:Binding Sites; D003062:Codon / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D011131:Polyribonucleotides / Q000096:biosynthesis*; D011130:RNA Ligase (ATP) / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis*
D003062:Codon; D011131:Polyribonucleotides; D012333:RNA, Messenger; D012335:RNA, Ribosomal; C008951:poly AUG; D012343:RNA, Transfer; D011130:RNA Ligase (ATP)