Effects of cytoskeleton-disrupting agents on tyrosine transport into cultured bovine adrenal chromaffin cells.
The effects of various cytoskeleton-disrupting agents on tyrosine transport into chromaffin cells were examined to assess the possibility of cytoskeleton involvement in the regulation of precursor supply for catecholamine synthesis. Tyrosine transport was markedly increased by cytochalasin B. Vinblastine also stimulated tyrosine transport, although its effect was less pronounced than that of cytochalasin B. While colchicine failed to cause any significant increase in the transport under the same conditions. These results therefore suggest a possible role of microfilaments as a factor regulating tyrosine transport into chromaffin cells.
Department of Pharmacology, Tokushima University School of Medicine, Japan.
K
K
Morita
K
K
Teraoka
M
M
Oka
S
S
Hamano
D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D002838:Chromaffin System / Q000187:drug effects* / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects*; D003847:Deoxyglucose / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*; D014747:Vinblastine / Q000494:pharmacology
D003571:Cytochalasin B; D014443:Tyrosine; D014747:Vinblastine; D003847:Deoxyglucose