Renal handling of 2-methyl-4-chlorophenoxyacetic acid (MCPA) in rats.
Following i.p. administration of various doses of 2-methyl-4-chlorophenoxyacetic acid (MCPA), ca. 50% is excreted during a 5-h diuresis experiment. After i.p. administration of MCPA, virtually no distribution occurs (Vrel = 18% of the body weight). Renal excretion of MCPA can be accelerated by inhibition of its renal tubular reabsorption. The distinct inhibition of renal excretion of MCPA by simultaneous administration of probenecid or p-aminohippurate (PAH) indicates the active tubular transport of MCPA; this transport process can be stimulated by treatment of rats with triiodothyronine. Active tubular transport of MCPA was confirmed by measurement of MCPA accumulation in renal cortical slices, both under aerobic and anaerobic conditions. Accumulation of MCPA under anaerobic conditions indicates an additional passive uptake and binding of MCPA in kidney tissue in accordance with the high degree of binding to plasma albumin (85%).
Institute of Pharmacology and Toxicology, Friedrich Schiller University, Jena, GDR.
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Bräunlich
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Bernhardt
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Bernhardt
D008456:2-Methyl-4-chlorophenoxyacetic Acid / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine*; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D004231:Diuresis / Q000187:drug effects; D005260:Female; D006016:Glycolates / Q000652:urine*; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism; D011339:Probenecid / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D014284:Triiodothyronine / Q000494:pharmacology; D010130:p-Aminohippuric Acid / Q000494:pharmacology
D001798:Blood Proteins; D006016:Glycolates; D014284:Triiodothyronine; D008456:2-Methyl-4-chlorophenoxyacetic Acid; D011339:Probenecid; D010130:p-Aminohippuric Acid