Expression of messenger RNA species coding for a Mr 43,000 peptide associated with ferritin in human leukemia-K562 cells and its down regulation during differentiation.
In the current study it was found that K-562 erythroleukemia cells express the superheavy (S) mRNA and the 43 Kd S peptide which were recently discovered in activated T-lymphocytes. The S mRNA was identified by its cross-hybridization with ferritin H complementary DNA probe and the S peptide (Mr 43,000) was identified by its immunoprecipitation with CM-H-9 monoclonal antibody specific for placental isoferritin. During terminal differentiation by hemin the level of S mRNA decreased below detection, with a concomitant diminution in the biosynthesis of the S peptide. In contrast, a significant increase in the level of ferritin light (L) and heavy (H) mRNAs was observed, resulting in an increase in the rate of biosynthesis of the H and L ferritin subunits. Removal of iron by desferrioxamine reduced the biosynthesis of ferritin L and H chains without altering the level of the corresponding mRNAs. Treatment with desferrioxamine did not affect either the level of S mRNA or the biosynthesis of the S peptide. This is a demonstration of a new mRNA species which is expressed in leukemic cells and is down-regulated during cell differentiation.
Rogoff Medical Research Institute, Beilinson Medical Center, Petah-Tikva, Israel.
N
N
Shterman
B
B
Kupfer
C
C
Moroz
D002460:Cell Line; D003676:Deferoxamine / Q000494:pharmacology; D005293:Ferritins / Q000096:biosynthesis / Q000235:genetics*; D006418:Heme / Q000494:pharmacology; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D006418:Heme; D005293:Ferritins; D003676:Deferoxamine