gamma-Aminobutyric acid regulation of neurohypophysial hormone secretion in male and female rats.
The role of gamma-aminobutyric acid (GABA) in the control of oxytocin and arginine vasopressin (AVP) release from the posterior pituitary was investigated using the GABA agonist muscimol and the GABA antagonists bicuculline and picrotoxin. Two perifusion model systems were studied using (a) intact isolated posterior pituitaries (IPP) and (b) neurosecretosomes from both male and female rats. In experiments on tissue from male rats, the stimulated release of oxytocin and AVP in both models was inhibited by muscimol, an effect which was reversed in the presence of bicuculline. Bicuculline alone increased the release of oxytocin only. Although similar responses to muscimol or bicuculline were seen in neurosecretosomes from female animals, neither agent affected oxytocin and AVP release from the intact IPP. Picrotoxin had a similar effect to bicuculline on oxytocin in isolated posterior pituitaries from male as well as female rats, although at the neurosecretosome level a paradoxical inhibition was observed. These results provide evidence for an endogenous GABA receptor mechanism at the level of the neurosecretory terminals in both male and female rats. The sexually dimorphic IPP response suggests a second more complex mechanism involving either pituicytenerve terminal interactions and/or a secondary role of other neurotransmitters in the GABA regulation of neurohypophysial hormones.
Medical Unit, Charing Cross and Westminster Medical School, Westminster Hospital, London.
E
E
Saridaki
D A
DA
Carter
S L
SL
Lightman
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D001640:Bicuculline / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D018756:GABA Antagonists; D008297:Male; D009118:Muscimol / Q000494:pharmacology; D010121:Oxytocin / Q000378:metabolism*; D010852:Picrotoxin / Q000494:pharmacology; D010904:Pituitary Gland, Posterior / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014701:Veratridine / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D001127:Arginine Vasopressin; D001640:Bicuculline; D018756:GABA Antagonists; D009118:Muscimol; D010121:Oxytocin; D010852:Picrotoxin; D014701:Veratridine; D005680:gamma-Aminobutyric Acid