Purine compounds in mitochondria: a quantitative evaluation.
Applying a recently developed reverse-phase ion-pair HPLC technique we determined purine compounds in acid extracts of mitochondria isolated from rat and mouse liver, rat renal cortex, and hepatoma. Intramitochondrial purine nucleosides and bases were evaluated quantitatively for the first time. All mitochondria examined contain significant amounts of adenosine, guanosine, inosine, guanine, and hypoxanthine. Moreover, IMP was identified in all extracts. Adenine was detected in traces only. These results are in accordance with the concept of mitochondrial metabolism of purine compounds.
Institute of Biochemistry, Humboldt University Berlin, GDR.
M
M
Ziegler
W
W
Dubiel
A M
AM
Pimenov
Tikhonov YuV
R T
RT
Toguzov
W
W
Henke
G
G
Gerber
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007672:Kidney Cortex / Q000032:analysis; D008114:Liver Neoplasms, Experimental / Q000032:analysis; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008928:Mitochondria / Q000032:analysis*; D008930:Mitochondria, Liver / Q000032:analysis; D011685:Purine Nucleotides / Q000302:isolation & purification*; D011687:Purines / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D011685:Purine Nucleotides; D011687:Purines