Recovery of hippocampal cholinergic activity by transplantation of septal neurons in AF64A treated rats.
Embryonic septal neurons were transplanted into the hippocampus of adult rats which had received lateral-ventricular administration of AF64A, a cholinergic neurotoxin, and the effects on hippocampal cholinergic activity were studied. One week after AF64A administration, we injected dissociated septal cell suspension into the dorsal hippocampus, unilaterally. About 3 months after the transplantation, acetylcholine (ACh)-rich septal grafts formed extensive acetylcholinesterase (AChE)-positive fibers into the host hippocampus, recovering choline acetyltransferase (ChAT) level only in the grafted side. These results indicate that septal implants can produce a partial recovery of the cholinergic activity in the chemically damaged hippocampus.
Department of Neuroscience, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
S
S
Ikegami
I
I
Nihonmatsu
H
H
Hatanaka
N
N
Takei
H
H
Kawamura
D000110:Acetylcholinesterase; D000818:Animals; D001388:Aziridines / Q000008:administration & dosage / Q000494:pharmacology*; D001389:Azirines / Q000494:pharmacology*; D002794:Choline / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D007276:Injections, Intraventricular; D008297:Male; D009466:Neuromuscular Blocking Agents / Q000008:administration & dosage / Q000494:pharmacology*; D009474:Neurons / Q000637:transplantation*; D051381:Rats; D011919:Rats, Inbred Strains; D014184:Transplantation, Homologous
D001388:Aziridines; D001389:Azirines; D009466:Neuromuscular Blocking Agents; C044894:ethylcholine aziridinium; D000110:Acetylcholinesterase; D002794:Choline