Effects of palmitate on astrocyte amino acid contents.
The effects of palmitate on intracellular and extracellular amino acid concentrations of cultured astrocytes was studied. Exposure of astrocytes to either 0.72 mM or 0.36 mM palmitate was associated with a significant reduction in the intracellular pool of glutamine and taurine. In contrast, the intracellular concentration of histidine, glycine, citrulline, isoleucine and leucine were increased in the presence of 0.72 mM palmitate. Comparable changes in the extracellular amino acid pool were not observed. The data suggest that palmitic acid, which accumulates in the brain during periods of anoxia, alters the metabolism of several amino acids in cultured astrocytes. These changes may be of significance in terms of the pathophysiology of a stress such as anoxia.
Division of Metabolism, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine 19104.
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Yudkoff
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Stern
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Pleasure
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology*; D051381:Rats
D000596:Amino Acids; D010169:Palmitic Acids; D019308:Palmitic Acid