[An approach for determining the capacity of human cells to repair gamma-induced DNA damage using interferon].
The method of ultracentrifugation of a nucleoid in a neutral sucrose gradient in the presence of ethidium bromide was used to detect gamma radiation-induced DNA breaks and their resynthesis in human HEp-2 cells and fibroblasts taken from a skin biopsy of patients with homocystinuria (HCN). In HEp-2 cells pretreated with interferon the nucleoid sedimentation rate after gamma irradiation did not differ from that in intact cells, that is, interferon exerted its protective effect whereas in HCN cells interferon was ineffective. After incubation with interferon, the resynthesis of the induced breaks was enhanced in these cells as well.
Shoniia
N N
NN
Sinel'shchikova
T A
TA
Zasukhina
G D
GD
D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects*; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D006712:Homocystinuria / Q000378:metabolism; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D014407:Tumor Cells, Cultured
D007370:Interferon Type I; D004247:DNA
Podkhod dlia opredeleniia sposobnosti kletok cheloveka k reparatsii gamma-indutsirovannykh povrezhdeniĭ DNK s pomoshch'iu interferona.