Defective T-cell surface antigen expression after mitogen stimulation. An index of lymphocyte dysfunction after controlled murine injury.
Murine spleen T-cell activation in lectin-stimulated cultures after 25% body surface area burn injury or hind-limb amputation was studied by measuring the temporal expression of cell surface markers using monoclonal antibodies and two-color flow cytometry. Lymphocyte activation has been shown to be accompanied by the appearance of new surface antigens, including Interleukin-2 (IL-2) deceptor (IL-2R) and Ia, and emergence of cells that coexpress helper (Th) and suppressor (Ts) surface markers. IL-2R has been shown to appear early on stimulated cells, before DNA synthesis, whereas Ia appears later. Surface markers (L3T4, Lyt2, Ia, and IL-2R) were analyzed at time 0 and after 24, 48, and 72 hours of mitogen-stimulated culture. The appearance of IL-2R and Ia on Th (L3T4+) and Ts (Lyt-2+) populations was markedly depressed after burn injury, but minimal changes were seen after musculoskeletal injury. In addition, coexpression of L3T4/Lyt2 antigens was markedly reduced in burn-derived cells. Serum from burn-injured animals caused depression of surface antigen expression by stimulated normal cells. Recombinant IL-2, when added to burn-derived cell cultures, did not increase expression of these surface markers during culture, nor did it improve proliferation.
Department of Surgery, University of California, San Diego.
M A
MA
Gadd
J F
JF
Hansbrough
D B
DB
Hoyt
N
N
Ozkan
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002056:Burns / Q000276:immunology; D002478:Cells, Cultured / Q000187:drug effects; D005260:Female; D005269:Femur / Q000293:injuries; D005434:Flow Cytometry; D007111:Immunity, Cellular; D007376:Interleukin-2 / Q000032:analysis / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D011948:Receptors, Antigen, T-Cell / Q000187:drug effects; D011994:Recombinant Proteins / Q000032:analysis / Q000494:pharmacology; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014947:Wounds and Injuries / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015415:Biomarkers; D007376:Interleukin-2; D037102:Lectins; D011948:Receptors, Antigen, T-Cell; D011994:Recombinant Proteins