Conformational change of band 3 protein induced by diethyl pyrocarbonate modification in human erythrocyte ghosts.
Diethyl pyrocarbonate inhibited the phosphate exchange across the human erythrocyte membrane. The exchange rate was inhibited only when the membranes were modified with the reagent from the cytosolic surface of resealed ghosts. The intracellular modification by diethyl pyrocarbonate inhibited the extracellular binding of [3H]dihydro-4,4'-diisothiocyanostilbene-2,2'-disulfonic acid to band 3 protein. Furthermore, the extracellular 4,4'-dinitrostilbene-2,2'-disulfonic acid protected the membranes from the intracellular modification by diethyl pyrocarbonate. These results suggest that the extracellular binding of 4,4'-dinitrostilbene-2,2'-disulfonic acid to band 3 protein induces the conformational change of the intracellular counterpart of band 3 protein and the diethyl pyrocarbonate susceptible residue(s) is (are) hidden from the cytosolic surface of the cell membrane in connection with the conformational change. Conversely, under the conditions where the diethyl pyrocarbonate modification is confined to the intracellular side of the membrane, the extracellular binding site of [3H]dihydro-4,4'-diisothiocyanostilbene-2,2'-disulfonic acid is hidden from the cell surface.
Department of Clinical Chemistry, Fukuoka University School of Medicine, Japan.
K
K
Izuhara
K
K
Okubo
N
N
Hamasaki
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D005561:Formates / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000378:metabolism; D011487:Protein Conformation; D013267:Stilbenes / Q000378:metabolism / Q000494:pharmacology; D013499:Surface Properties
D000596:Amino Acids; D001457:Anion Exchange Protein 1, Erythrocyte; D005561:Formates; D010710:Phosphates; D013267:Stilbenes; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C022306:4,4'-dinitro-2,2'-stilbenedisulfonic acid; D004047:Diethyl Pyrocarbonate; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid