Use of terpene-based solvents (Hemo-De, Histoclear, and Shandon and BDH xylene substitutes) in place of xylene in the Ehrlich indole test.
Although less effective than xylene and Kovacs reagent as indole extractants, Hemo-De and Shandon xylene substitute can be used in the Ehrlich indole test if a less toxic solvent is desirable. In doubtful cases, xylene should be used. Because of the blocking action shown in the test, use of indole-nitrite broth cannot be recommended.
Provincial Laboratory of Public Health for Southern Alberta, Calgary, Canada.
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Gubash
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Bennett
D001419:Bacteria / Q000378:metabolism*; D006801:Humans; D007211:Indoles / Q000302:isolation & purification / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism*; D014992:Xylenes / Q000378:metabolism*
D007211:Indoles; D013729:Terpenes; D014992:Xylenes