Monoclonal antibodies against rat glutathione S-transferase isoenzymes 2-2 and 3-3.
Monoclonal antibodies were prepared against rat glutathione S-transferase isoenzymes 2-2 and 3-3. Three clones were capable of specifically differentiating their respective antigens from other rat isoenzymes as well as human isoenzymes, in ELISA and on Western blot. One clone produced antibodies specific for isoenzyme 2-2, and 2 hybridomas were specific for isoenzyme 3-3. Balb/c mice did not respond to immunization with glutathione S-transferase isoenzymes 1-1 and 4-4. However, an immune response was obtained in some other strains of mice, with differential H-2 haplotypes, notably CBA/BrARij mice and CBA/CaHRij-T6 mice for isoenzyme 1-1 and CBA/BrARij mice for isoenzyme 4-4, which offers perspectives for obtaining additional specific monoclonal antibodies against these glutathione S-transferases.
Agricultural University, Department of Toxicology, Wageningen, The Netherlands.
R M
RM
Vos
I J
IJ
Lutkeschipholt
P M
PM
Boonekamp
A
A
Schots
H
H
Pomp
P J
PJ
Van Bladeren
J H
JH
Koeman
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003429:Cross Reactions; D005982:Glutathione Transferase / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007527:Isoenzymes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D005982:Glutathione Transferase