An enzyme immunoassay for isotyping mouse monoclonal antibodies.
A rapid, simply performed and relatively inexpensive enzyme immunoassay for isotyping mouse monoclonal antibodies is described, based on the urease/urea system. Because of the high sensitivity (less than 0.1 microgram/ml of immunoglobulin can be detected in cell culture medium) no treatment of the hybridoma supernatant sample is required prior to assay, and the isotype of a mouse immunoglobulin can be determined in about thirty minutes.
Coulter
A R
AR
Cox
J C
JC
Harris
R D
RD
Healey
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification*; D004797:Enzyme-Linked Immunosorbent Assay; D007132:Immunoglobulin Isotypes / Q000032:analysis*; D051379:Mice
D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes