Spatial and temporal variability of blood flow in stimulated dog gastrocnemius muscle.
The distribution of blood flow in skeletal muscle stimulated to rhythmic isotonic contractions was studied by injections of radioactive microspheres into the arterial supply of gastrocnemius muscles (mean weight 88 g) subsequently cut into 0.5 g pieces for determination of radioactivity. The coefficient of variation (CV = SD/mean) of the ratio of simultaneously injected 10 microns and 15 microns microspheres, 0.12, was taken as the inherent scatter of the method. The average spatial distribution inequality of 10-15 microns microspheres corresponded to a CV of 0.45 and the specific local blood flow inhomogeneity to a CV = 0.43 (= square root of 0.45(2) - 0.12(2)), but there were marked differences between muscles. The temporal variability of blood flow in individual muscle pieces was obtained from the comparison of fractional trapping of 4 to 5 differently labeled microspheres injected at intervals of 2 minutes into steadily stimulated muscles. The mean CV for the variations in time was 0.23 and that corrected for methodological scatter, 0.19. There were large differences between muscle pieces within a muscle and between muscles. The presence of considerable spatial and temporal variations of blood flow in exercising muscle during apparent steady state may be important in limiting and/or modulating tissue O2 supply.
Abteilung Physiologie, Max-Planck-Institut für experimentelle Medizin, Göttingen, F.R.G.
J
J
Piiper
C
C
Marconi
N
N
Heisler
M
M
Meyer
H
H
Weitz
D R
DR
Pendergast
P
P
Cerretelli
D000818:Animals; D001158:Arteries / Q000502:physiology; D004285:Dogs; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008863:Microspheres; D009119:Muscle Contraction; D009132:Muscles / Q000098:blood supply* / Q000502:physiology; D012039:Regional Blood Flow