Isolation of myelin bodies from the kidney cortex of gentamicin-treated rats.
Myelin bodies have been isolated from the kidney cortex of gentamicin-treated rats (100 mg gentamicin sulfate/kg body weight i.p. twice daily for 3 days) by a simple procedure involving differential centrifugation followed by equilibrium density centrifugation on a discontinuous sucrose gradient. Electron microscopy and assay of acid phosphatase suggest that the myelin bodies were obtained in virtually quantitative yield, essentially uncontaminated by other cellular structures and relatively intact. The method developed here may also prove applicable for the isolation of myelin bodies arising in connection with other drug treatments and may provide information on a number of toxic side-effects of clinical importance.
Department of Biochemistry, University of Stockholm, Sweden.
L
L
Nässberger
A
A
Bergstrand
J W
JW
DePierre
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D005839:Gentamicins / Q000633:toxicity*; D007425:Intracellular Membranes / Q000648:ultrastructure; D007672:Kidney Cortex / Q000187:drug effects* / Q000648:ultrastructure; D008563:Membrane Lipids / Q000032:analysis; D008854:Microscopy, Electron; D051381:Rats
D005839:Gentamicins; D008563:Membrane Lipids; D000135:Acid Phosphatase