An enzyme-linked immunosorbent assay (ELISA) for the measurement of serum antibodies to growth hormone. The Wessex Growth Study.
A solid-phase enzyme-linked immunosorbent assay for measuring antibodies to growth hormone (GH) was developed, with occasional use and long shelf-life in mind. With coating protein concentration optimised, the standard curve was linear over a 25-fold range of concentration. The interassay coefficient of variation ranged from 14.4% to 9.3%. The specificity of the assay was confirmed by the substitution of insulin for GH as coating protein and by preabsorption of sera with GH coupled covalently to Sepharose beads. There was no binding signal with insulin and absorption was dose dependent, maximum (greater than 80% complete) with 2 x 10(4) micrograms GH/ml undiluted serum. The assay was simple to perform, cheap and reliable.
Department of Medicine II, General Hospital, Southampton, U.K.
T
T
Wilkin
C
C
Casey
A
A
Tuck
P
P
Englefield
D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D013006:Growth Hormone / Q000276:immunology* / Q000627:therapeutic use; D006801:Humans; D011863:Radioimmunoassay; D013997:Time Factors
D000906:Antibodies; D013006:Growth Hormone