[Comparison of the results of radioimmunologic and hemagglutination methods in the determination of antibodies against thyroglobulin and the effect of these antibodies on thyroglobulin serum levels].
The following methods have been introduced at our institute: labeling of human thyroglobulin (H-Tg) with the radioiodine 125I by the lactoperoxidase method, radioimmunologic method for serum H-Tg determination by means of rabbit antiserum to H-Tg prepared at our institute, and radioimmunologic method for the determination of antibodies to thyroglobulin. Sera from 15 patients with different thyropathies were examined by the given methods. In the first part of the work the quality of 125I labeled H-Tg was studied. The maximum binding by antiserum was found to be substantially decreased as early as two weeks following labeling, The second part of the study presents our first experience with comparing our RIA method and the hemagglutination method for TgAb determination. The results yielded by the two methods did not differ significantly. The level of serum H-Tg is falsely affected by the competition of TgAb autoantibodies with the first rabbit antibody to H-Tg at recipitation by means of the second antibody. It is therefore important to establish the titer of autoantibodies before actual serum H-Tg determination. This approach is of importance e.g. in following up patients with malignant goiter. The introduced methods are a contribution to diagnosis and management of patients with thyropathies.
Resetková
E
E
Strbák
V
V
Hnilica
P
P
Zórad
S
S
D001323:Autoantibodies / Q000032:analysis*; D006386:Hemagglutination Tests; D006801:Humans; D011863:Radioimmunoassay; D013954:Thyroglobulin / Q000097:blood / Q000276:immunology*
D001323:Autoantibodies; D013954:Thyroglobulin
Porovnanie výsledkov rádioimunologickej a hemaglutinacnej metódy na urcenie protilátok proti tyreoglobulínu a ovplyvnenie jeho hladiny v sére prítomnost'ou týchto látok.