Liposome-mediated delivery of gadolinium-diethylenetriaminopentaacetic acid to hepatic cells: a P-31 NMR study.
Delivery of gadolinium-diethylenetriaminopentaacetic acid (Gd-DTPA) (a magnetic resonance imaging contrast agent) to hepatic cells via liposomes used as carriers was demonstrated and monitored by P-31 NMR spectroscopy at 162 MHz on a perfused rat liver model. The penetration of Gd-DTPA into hepatic cells was reflected by a 41% reduction in the longitudinal relaxation time of the beta phosphorus atom of intrahepatic ATP used as an index of the contrast agent presence. The perfusion of free Gd-DTPA did not affect the relaxation index nor did liposomes devoid of paramagnetic agent. There was no indication of interference of Gd-DTPA, free or encapsulated in liposomes, with liver energetic metabolism. Alterations of hepatic magnetic resonance imaging signal induced by Gd-DTPA containing liposomes might help to assess the functional state of the parenchyma and pinpoint the intracellular compartment as opposed to the intravascular space.
Vion-Dury
J
J
Centre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique (URA No. 1186), Faculté de Médecine Timone, Marseille, France.
Masson
S
S
Devoisselle
J M
JM
Sciaky
M
M
Desmoulin
F
F
Confort-Gouny
S
S
Coustaut
D
D
Cozzone
P J
PJ
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D019786:Gadolinium DTPA; D066298:In Vitro Techniques; D008081:Liposomes; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004369:Pentetic Acid / Q000008:administration & dosage* / Q000493:pharmacokinetics; D010477:Perfusion; D051381:Rats
D008081:Liposomes; D009942:Organometallic Compounds; D004369:Pentetic Acid; D000255:Adenosine Triphosphate; D019786:Gadolinium DTPA