Development of an assay for human growth hormone in urine using commercially available reagents.
An assay for the measurement of human growth hormone in urine (u-HGH) has been developed using commercially available reagents and its analytical variables have been investigated. The procedure is sensitive to 0.50 muU/L urine, has an interassay precision of 6.2-7.5 (CV%) over the standard curve, and shows good parallelism and recovery. Urine samples for the assay can be collected in plain plastic bottles, stored at room temperature for up to 24 h and, after adding preservative, at -20 C before assay for three months. There was a good correlation between the responses of HGH in serum and in urine during clonidine and insulin stimulation tests.
Evans
A J
AJ
Department of Chemical Pathology, Southampton General Hospital, Southampton, Hants, UK.
Wood
P J
PJ
D002648:Child; D005615:Freezing; D013006:Growth Hormone / Q000097:blood / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D011309:Preservation, Biological; D011933:Reagent Kits, Diagnostic; D013696:Temperature; D013997:Time Factors
D011933:Reagent Kits, Diagnostic; D013006:Growth Hormone