Reversed-phase high-performance liquid chromatographic method for the quantification of 5-hydroxymethylfurfural as the major degradation product of glucose in infusion fluids.
Analysis of 5-hydroxymethylfurfural (5-HMF) is an important indicator of degradation in glucose infusion fluids. Current pharmacopoeial methods for analysis are insensitive and non-specific. A method is described here, incorporating an internal standard, to give a sensitive, accurate and precise method, suitable for use in the quality control of glucose infusion fluids, and with possible applications for the quantitative determination of 5-HMF in food and drink, bacterial cultures and hydrothermolysed wood.
Cook
A P
AP
Area Pharmaceutical Laboratory, Ninewells Hospital, Dundee, U.K.
Macleod
T M
TM
Appleton
J D
JD
Fell
A F
AF
D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D005947:Glucose / Q000008:administration & dosage / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet; D013242:Sterilization
C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; D005947:Glucose