[Characteristics of the cis- and trans-positions of chromatids of human acrocentric chromosomes in extreme old age].
Cis- and trans-positions of chromatid associations of human acrocentric chromosomes were examined at extreme old age. Lymphocyte cultures were prepared by the usual method, from peripheral blood of 9 subjects aged 80-90 years (analysis of 179 metaphases) and 7 subjects aged 20-48 years (analysis of 124 metaphases). The functional difference between stalks of the sister chromatids was found. In the subjects at the age 80-90 years satellite stalks of chromatids-1 (in all DNA strands thymidine was substituted by 5-BrdU) of the D chromosome in cis-position are included into associations with lower frequency, as compared with the satellite stalks of chromatids-2 (thymidine+ is only substituted by 5-BrdU in a half DNA strands of the chromosome). This apparently reflects variability of regulation of functional activity of satellite stalks of sister chromatids.
Lezhava
T A
TA
Khmaladze
E V
EV
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics*; D002842:Chromatids; D002877:Chromosomes, Human; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000166:cytology / Q000648:ultrastructure; D008677:Metaphase; D008875:Middle Aged
Kharakteristika tsis- i trans-polozheniĭ assotsiiruiushchikh khromatid akrotsentricheskikh khromosom cheloveka v glubokoĭ starosti.