Inhibitory effect of Toxoplasma lysate antigen on the multiplication of transplanted tumor in mice.
Tachyzoites of the RH strain of Toxoplasma gondii were pretreated by a process of freezing and thawing followed by ultrasonication. After ultracentrifugation at 144,000 g for 120 minutes, the resulting supernatant contained a protein (TLA 144), which consisted mainly of a component of protozoan origin with a molecular weight of 10,000-20,000, and additional sugars, peptides, and amino acids. TLA 144 was soluble in water and of very low toxicity. Mice that had been inoculated with allogeneic (S-180) and syngeneic (Meth A) transplantable tumor cells, were injected intramuscularly with 100 micrograms of TLA 144 once a week for some time beginning one week after transplantation. It was found that after TLA treatment the multiplication of tumor cells was more intensely inhibited than following administration of OK-432, one of the biological response modifiers (BRMs).
Dept. of Veterinary Physiology, Obihiro University, Yagi Hospital, Hyogo, Japan.
A
A
Saito
H
H
Sakurai
S
S
Terada
I
I
Igarashi
T
T
Hirose
N
N
Suzuki
H
H
Osaki
H
H
Kudo
Y
Y
Sakata
K
K
Okamoto
D000818:Animals; D000953:Antigens, Protozoan; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D012513:Sarcoma, Experimental / Q000188:drug therapy*; D014122:Toxoplasma
D000953:Antigens, Protozoan; D000970:Antineoplastic Agents