Production of a toxic shock syndrome toxin variant by Staphylococcus aureus strains associated with sheep, goats, and cows.
A toxic shock syndrome toxin (TSST) variant with an isoelectric point (pI) of 8.6 produced by an ovine-associated Staphylococcus aureus strain was described previously. Analysis of additional strains associated with sheep, goats, cows, and humans by isoelectric focusing with immunoblotting using monoclonal antibodies revealed that all 18 strains associated with sheep and all 12 strains associated with goats produced the TSST variant. Only 1 of 10 bovine-associated strains and no human-associated strains produced the variant, whereas the others produced TSST-1 (pI between 7.0 and 7.2). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting indicated that both TSST-1 and the TSST variant had a molecular size of 24 kilodaltons.
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Department of Biological Sciences, Michigan Technological University, Houghton 49931.
Campbell
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Bergdoll
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Carlson
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D000818:Animals; D001427:Bacterial Toxins; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000096:biosynthesis*; D006041:Goats; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008413:Mastitis / Q000382:microbiology / Q000662:veterinary; D008414:Mastitis, Bovine / Q000382:microbiology; D008970:Molecular Weight; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology; D012772:Shock, Septic / Q000382:microbiology; D013211:Staphylococcus aureus / Q000378:metabolism*; D018089:Superantigens
D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal