Use of nasal and pharyngeal swabs for rapid detection of respiratory syncytial virus and adenovirus antigens by enzyme-linked immunosorbent assay.
Nasal and pharyngeal swabs from 134 children with acute respiratory diseases were examined for the presence of respiratory syncytial (RS) virus and adenovirus antigens by enzyme-linked immunosorbent assay (ELISA). The results were compared with those obtained by virus isolation and serology. Altogether, 56 RS virus-positive (prospective study), 51 adenovirus-positive (retrospective study), and 27 negative (prospective study) samples were examined. The sensitivities of ELISA were 96 and 98% for RS virus and adenovirus antigen detection, respectively. No false-positive results were observed. It is concluded that the joint eluate from a nasal and a pharyngeal swab, properly taken and handled, serves well for the detection of RS virus and adenovirus acute respiratory infections by ELISA.
Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
M
M
Brůcková
M
M
Grandien
C A
CA
Pettersson
L
L
Kunzová
D000258:Adenovirus Infections, Human / Q000382:microbiology; D000260:Adenoviruses, Human / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006801:Humans; D007223:Infant; D009297:Nasal Mucosa / Q000382:microbiology*; D010614:Pharynx / Q000382:microbiology*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D012140:Respiratory Tract Diseases / Q000382:microbiology; D010253:Respirovirus Infections / Q000382:microbiology; D012189:Retrospective Studies
D000956:Antigens, Viral