Iron depletion alters surface-associated properties of Staphylococcus aureus and its association to human neutrophils in chemiluminescence.
Staphylococcus aureus NCTC 6571 was grown in iron-depleted tryptone soya broth (Fe-TSB) to approximate to in vivo conditions, and in iron-rich TSB (Fe + TSB). Low iron effected a crucial decrease in surface hydrophobicity (SH) and a lack of supernatant Protein A (PrA). Iron availability did not affect PrA detection in immunoblotting and it was identified as a 35.5 kDa antigen in this strain. Fe-phenotypes lacked 34, 48 and 52 kDa antigens. In chemiluminescence, Fe-phenotypes appeared least vulnerable to phagocytosis despite opsonisation.
Domingue
P A
PA
Department of Pharmaceutical Sciences, Aston University, Birmingham, U.K.
Lambert
P A
PA
Brown
M R
MR
D000954:Antigens, Surface; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects; D013205:Staphylococcal Protein A / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D000954:Antigens, Surface; D013205:Staphylococcal Protein A; D007501:Iron