Heterogeneity of a crude synaptosomal preparation, studied by affinity partitioning using hexaethonium-poly(ethylene glycol).
The heterogeneity of a synaptosomal preparation was studied by the use of affinity partitioning in combination with centrifugal counter-current distribution. Hexaethonium-poly(ethyleneglycol) was used as the extracting agent. The fractions were analyzed for: light scattering, protein, choline acetyltransferase, L-glutamate decarboxylase, glutamine synthetase, 2',3'-cyclicnucleotide-3'-phosphohydrolase, acetylcholinesterase and succinate dehydrogenase. The material was fractionated into three main fractions which differed in their content of marker-enzymes.
Olde
B
B
Department of Biochemistry, Chemical Center, University of Lund, Sweden.
Johansson
G
G
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods; D002498:Centrifugation; D003377:Countercurrent Distribution; D004797:Enzyme-Linked Immunosorbent Assay; D010783:Photometry; D011092:Polyethylene Glycols; D000644:Quaternary Ammonium Compounds; D013574:Synaptosomes
D000644:Quaternary Ammonium Compounds; D011092:Polyethylene Glycols