Recovery of human immunodeficiency virus type 1 from supernatant fluid of routine viral cultures.
Peripheral blood mononuclear cell samples from human immunodeficiency virus (HIV) antibody-positive subjects were inoculated into cell cultures used for routine viral isolation to determine if HIV could survive in them. HIV was recovered from 5 (19%) of 26 day 1 supernatants and from four of the five samples in all three types of cell cultures. We conclude that HIV may survive for at least 24 h in standard virology cell culture systems and therefore is a potential risk for laboratory personnel.
Department of Laboratory Medicine and Pathology, University of Minnesota Health Sciences Center, Minneapolis, 55455.
E O
EO
Sia
J A
JA
Englund
K
K
Sannerud
K
K
Hansen
N
N
Shah
H H
HH
Balfour
D000163:Acquired Immunodeficiency Syndrome / Q000635:transmission*; D002478:Cells, Cultured; D015497:HIV-1 / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology*