Demonstration of cell-ricin interaction by electrophoretic light scattering.
Electrophoretic light scattering has been used to investigate the interaction of ricin, a vegetal toxin, with cells. This technique allowed measurements in the presence of free ligand and proved particularly useful for the study of a system with low affinity. The electrophoretic mobility of erythrocytes and oligodendrocytes was found equal to 2.08 x 10(-8) and 2.35 x 10(-8)m2s-1V-1, respectively. Upon ricin binding, these values decreased significantly. This change was related to the saturation of the binding sites. The specificity of the interaction was demonstrated by conducting the experiments in the presence of lactose. This specific inhibitor fully prevented the ricin-cell interaction.
Laboratoire de Diffusion Inélastique de la Lumière, UER Etudes Médicales et Biologiques, Paris, France.
B
B
Hakim Hachemi
C
C
Betrencourt
B
B
Volochine
J P
JP
Frénoy
Z
Z
Chraïbi
A
A
Alfsen
F
F
Lavialle
D001667:Binding, Competitive; D004301:Doppler Effect; D004586:Electrophoresis / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007785:Lactose / Q000494:pharmacology; D008027:Light; D009457:Neuroglia / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D037121:Plant Lectins; D010947:Plants, Toxic; D012276:Ricin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012278:Ricinus / Q000032:analysis; D012542:Scattering, Radiation
D037121:Plant Lectins; D012276:Ricin; D007785:Lactose