A unique deletion in pBR322 DNA caused by insertion of poly(dA).poly(dT).
A unique deletion covering around 43% of the pBR322 genome was found after attempting to insert 100 or 200 bp poly(dA).poly(dT) into the EcoRV site of pBR322 DNA. This result was not observed if an equivalent size heterologous DNA or a larger poly(dA).poly(dT) fragment of 10-20,000 bp was introduced at the same site. DNA sequencing analysis at the junctions suggests that a specific intramolecular pairing may be involved in the formation of this deletion mutant.
Division of Biomedical Sciences, Meharry Medical College, Nashville, Tennessee 37208.
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Ikpeazu
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Valenzuela
D001483:Base Sequence; D015139:Blotting, Southern; D002872:Chromosome Deletion; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011067:Poly dA-dT / Q000235:genetics*; D011089:Polydeoxyribonucleotides / Q000235:genetics*; D015183:Restriction Mapping; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT