Karyometric marker features in tissue adjacent to invasive cervical carcinomas.
A companion study showed the existence of statistically significant changes in the value of karyometric "marker features" in the nuclei of histologically normal-appearing ectocervical epithelium adjacent to carcinomas in situ. In this second part of the study, the results obtained in patients with invasive cervical carcinomas were analyzed. Formalin-fixed, paraffin-embedded tissue sections were Feulgen stained and analyzed with a microTICAS video microphotometer. The results, demonstrated for the first time in histologic material, indicate that the marker features are clearly expressed in a majority of nuclei observed in the normal-appearing tissue adjacent to invasive lesions. This effect was statistically significant. The best marker features selected by the discriminant analysis were nuclear roundness, nuclear perimeter length, total optical density and a run length texture measure. These findings may reflect a subtle transformation of the apparently normal cervical epithelium adjacent to an invasive cervical carcinoma.
Bibbo
M
M
Department of Obstetrics and Gynecology, University of Chicago, Illinois 60637.
Montag
A G
AG
Lerma-Puertas
E
E
Dytch
H E
HE
Leelakusolvong
S
S
Bartels
P H
PH
D002277:Carcinoma / Q000473:pathology*; D002467:Cell Nucleus / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D007621:Karyotyping; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
D004273:DNA, Neoplasm