Topical growth factors and wound contraction in the rat: Part II. Platelet-derived growth factor and wound contraction in normal and steroid-impaired rats.
The wound contraction model described in Part I was used to study topically applied, recombinant platelet-derived growth factor (rPDGF-BB) and wound contraction in normal and steroid-impaired rats. Circular wounds 4 cm in size were made on the dorsum of the rats with halothane anesthesia. Five groups of 20 normal rats each were treated with the vehicle alone and four concentrations of rPDGF-BB (0.5, 1.0, 3.0, and 10.0 micrograms/ml). Four groups of 20 steroid-impaired rats each were treated with the vehicle alone and three concentrations of rPDGF-BB (0.5, 1.0, and 3.0 micrograms/ml). The wounds were dressed with 2 x 2 gauze, Adaptic, 4 x 4 gauze, and Tubigrip. The investigators were blinded to the treatments until the completion of the experiment, and the treatments were applied in random order. Dressings were changed every 2 to 3 days for 14 days and the wound areas were determined, expressed as a fraction of the original, and plotted versus time. The areas under the curves and the means of the fraction open each day were compared between groups using Student's t-test. The treatment with 3.0-micrograms/ml rPDGF-BB accelerated the rate of wound contraction in the steroid-impaired animals (p less than 0.05).
Engrav
L H
LH
Division of Plastic Surgery, University of Washington, Seattle 98195.
Richey
K J
KJ
Kao
C C
CC
Murray
M J
MJ
D000287:Administration, Topical; D000818:Animals; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage*; D051381:Rats; D011994:Recombinant Proteins; D013256:Steroids / Q000502:physiology*; D014945:Wound Healing / Q000187:drug effects*
D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D013256:Steroids