A rapid procedure for cell colony hybridization using DNA probes.
A rapid, direct method for screening single cell-derived colonies or foci is described. The method allows the screening of a large number of colonies or foci by nitrocellulose filter hybridization using DNA probes. This technique simplifies current screening procedures and is a reliable, rapid, and sensitive method for the selection of cell clones containing a desired transfected gene.
Avraham
H
H
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Avraham
S
S
Bernstein
S C
SC
D000818:Animals; D002460:Cell Line; D015342:DNA Probes; D015151:Immunoblotting; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014162:Transfection
D015342:DNA Probes