The effect of ascorbic acid on the metabolism of rat calvarial bone cells in vitro.
Addition of 50 micrograms/ml sodium ascorbate to confluent cultures of isolated rat calvarium bone cells resulted in a 21% increase in DNA production, a 50-60% increase in incorporation of [14C]proline into collagenous and noncollagenous proteins, and a 200% increase in alkaline phosphatase activity; under identical conditions, [35S]sulfate incorporation into proteoglycans (glycosaminoglycans) was not affected. These results suggest that ascorbate may be important in maintaining or stimulating the osteogenic phenotype of normal bone cells.
Spindler
K P
KP
McKay Laboratories of Orthopaedic Research, University of Pennsylvania School of Medicine, Philadelphia 19104-6081.
Shapiro
D B
DB
Gross
S B
SB
Brighton
C T
CT
Clark
C C
CC
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000096:biosynthesis; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D006025:Glycosaminoglycans; D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid