Absence of homologous restriction factor does not affect CTL-mediated cytolysis.
Homologous restriction factor (HRF) is a membrane protein of erythrocytes and leukocytes that inhibits the complement (C5b-9)-mediated lysis in a species-restricting manner. HRF has also been reported to inhibit perforin-mediated cytolysis and postulated to play a role in cytotoxic T lymphocyte (CTL) self-protection. We show that paroxysmal nocturnal hemoglobinuria (PNH) erythrocytes, lacking HRF, are no more sensitive to Ca2+-dependent human CTL-mediated lysis than normal erythrocytes. Furthermore, mouse and normal human erythrocytes, as well as PNH erythrocytes, are similarly lysed by isolated murine perforin-containing granules. We conclude that HRF does not inhibit perforin-mediated lysis and therefore is not likely to play a role in CTL self-protection.
Institute of Biochemistry, Lausanne University, Epalinges, Switzerland.
O P
OP
Krähenbühl
H H
HH
Peter
J
J
Tschopp
D001798:Blood Proteins / Q000172:deficiency*; D018957:CD59 Antigens; D002352:Carrier Proteins; D003165:Complement System Proteins / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood / Q000276:immunology; D006461:Hemolysis; D006801:Humans; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000097:blood / Q000172:deficiency* / Q000502:physiology; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000502:physiology
D001798:Blood Proteins; D018957:CD59 Antigens; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; C051590:CD59 protein, human; D054353:Perforin; D003165:Complement System Proteins