Fast isoelectric focusing of milk proteins on small ultrathin polyacrylamide gels containing urea.
The preparation of 0.45 mm thin polyacrylamide gels, containing urea, for horizontal micro isoelectric focusing of milk proteins with PhastSystem is described. Isoelectric focusing in the small gels, stained either with Coomassie Brilliant Blue R-250 or with the more sensitive silver stain, affords a fast and sensitive procedure for an analysis of milk and cheese proteins. The procedure can be effectively exploited in detecting adulteration in ovine cheese with bovine milk.
Istituto Industrie Agrarie, Università di Napoli, Naples, Italy.
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Di Luccia
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Addeo
D000180:Acrylic Resins; D000665:Ampholyte Mixtures; D000818:Animals; D003720:Densitometry; D007525:Isoelectric Focusing / Q000379:methods*; D008894:Milk Proteins / Q000032:analysis*; D012834:Silver; D014508:Urea
D000180:Acrylic Resins; D000665:Ampholyte Mixtures; D008894:Milk Proteins; C016680:polyacrylamide gels; D012834:Silver; D014508:Urea