Villous sodium gradient associated with volume absorption in the feline intestine: an electron-microprobe study on freeze-dried tissue.
Water transport in biological tissue is driven by local osmotic gradients created by accumulation of actively transported ions in tissue compartments. To localize and measure such gradients, jejunal segments from the small intestine of anaesthetized cats were perfused with modified isotonic Krebs-Henseleit electrolyte solution, and net fluid transport was measured with a volumetric technique. The segments were then rapidly frozen, freeze-dried, and prepared for X-ray micro-analysis of elemental content. Whenever the lumen perfusate contained sodium, the apical third of the villus was found to have a sodium gradient rising to a tip concentration more than twice that at the base of the villus. This sodium gradient was associated with a chloride gradient and fluid absorption. No similar potassium gradient was found. When choline replaced sodium in the intestinal lumen, no gradient of sodium chloride was found and no net fluid absorption occurred. Absorption of fluid was thus apparently coupled to absorption of sodium through creation of a local osmotic gradient in the tip of the intestinal villus.
Department of Pharmacology, Smith, Kline & French Laboratories, King of Prussia, Pennsylvania.
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Sjöqvist
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Beeuwkes
D000818:Animals; D002415:Cats; D002794:Choline / Q000493:pharmacokinetics; D004577:Electron Probe Microanalysis; D005260:Female; D005612:Freeze Drying; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism*; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000493:pharmacokinetics; D012965:Sodium Chloride / Q000493:pharmacokinetics; D014882:Water-Electrolyte Balance
D012965:Sodium Chloride; D011189:Potassium Chloride; D002794:Choline