Phenotypic hiding: the carryover of mutations in RNA viruses as shown by detection of mar mutants in influenza virus.
When influenza virus monoclonal antibody-resistant (mar) mutants are selected by incubation in vitro with excess antibody, 90 to 99% of the mutants are not detectable. This observation may be explained by encapsidation of mar mutant RNAs within phenotypically wild-type envelopes. This phenotypic hiding can be revealed by selection of mar mutants in vivo after virus uncoating. Using experimental procedures appropriate to detect all viable mar mutants in a virus population, we determined precisely the mutation rates to the mar genotype by the fluctuation test for two nonoverlapping monoclonal antibodies.
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
J
J
Valcárcel
J
J
Ortín
D000911:Antibodies, Monoclonal / Q000276:immunology*; D009154:Mutation; D009975:Orthomyxoviridae / Q000235:genetics / Q000276:immunology*; D010641:Phenotype
D000911:Antibodies, Monoclonal