[5-Alkyl(C19-C25) resorcinols as regulators of succinate and NAD-dependent substrate oxidation by mitochondria].
The effect of 5-n-alkyl(C19-C25) resorcinols isolated from Azotobacter chroococcum on the oxidation of succinate and NAD-dependent substrates (glutamate, alpha-ketoglutarate, malate, pyruvate) by rat liver mitochondria was studied, using the polarographic technique. With succinate, the above resorcinol lipids activated to some extent the 2,4-dinitrophenol-decoupled mitochondrial respiration, but markedly suppressed it (up to 95%) in the presence of NAD-dependent substrates. The activating and inhibiting effects correlated with the resorcinol lipid/mitochondrial proteins ratio and were observed, when the lipid concentration in the incubation mixture ranged from 2.4.10(-4) to 6.0.10(-4) M. The most striking inhibiting effect was observed with alpha-ketoglutarate as substrate. The results obtained suggest that 5-n-alkyl(C19-C25) resorcinols should be regarded as rotenone type regulators of cell respiration.
Nenashev
V A
VA
Pridachina
N N
NN
Pronevich
L A
LA
Batrakov
S G
SG
D000818:Animals; D001395:Azotobacter / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D066298:In Vitro Techniques; D007656:Ketoglutaric Acids / Q000378:metabolism*; D008293:Malates / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012118:Resorcinols / Q000494:pharmacology*; D013386:Succinates / Q000378:metabolism*
D005971:Glutamates; D007656:Ketoglutaric Acids; D008293:Malates; D011773:Pyruvates; D012118:Resorcinols; D013386:Succinates; D009243:NAD
5-Alkil(C19-C25)rezortsiny--regulatory okisleniia mitokhondriiami suktsinata i NAD-zavisimykh substratov.