Effect of benfluron metabolites on ATP level in tumor cells.
Changes in ATP levels of both Ehrlich ascites carcinoma and P388 leukemia cells were evaluated after 2 h of incubation in the presence of different concentrations of benfluron (BF), 7-dihydrobenfluron (DBF) and N-oxide of benfluron (NOBF). Up to the concentration of 37.5 mumol/l, none of the substances significantly depressed the ATP levels. A more expressive decrease in ATP levels was noted only at concentrations of 75 mumol/l and higher. BF proved the most effective, less effective was DBF, while NOBF was practically without any effect. Of the remaining cytostatic drugs, Mitoxantrone, CCNU and Me-CCNU, in particular, proved efficient in depressing ATP level. The latter becomes depressed already after 15 min of incubation in the presence of the highest concentrations of benfluron and 7-dihydrobenfluron.
Department of Microbiology and Biochemistry, Slovak Polytechnical University, Bratislava, Czechoslovakia.
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Miko
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Krepelka
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Mĕlka
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Vajdová
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D005449:Fluorenes / Q000494:pharmacology*; D007700:Kinetics; D007941:Leukemia P388 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice
D000970:Antineoplastic Agents; D005449:Fluorenes; C034234:VUFB 13468; C059231:7-dihydrobenfluron; D000255:Adenosine Triphosphate; C059230:benfluron N-oxide