Identification of protein components of Echinococcus granulosus protoscolex antigens for specific serodiagnosis of E. granulosus infection in dogs.
Proteins of Echinococcus granulosus protoscolex excretory/secretory or deoxycholate solubilized somatic antigens were radiolabelled with 125I and immunoprecipitated with sera from dogs naturally or experimentally infected with E. granulosus and various control dog sera. Analysis of immunoprecipitates was performed using one- and two-dimensional gel electrophoresis to identify antigenic protein components specific for E. granulosus. Using both electrophoretic techniques, a basic component of Mr 27,000 and an acidic component of Mr 94,000 were defined in both excretory/secretory and somatic protoscolex antigens, and were specifically identified by 95% and 62% of 21 sera from E. granulosus-infected dogs, respectively. An abundant component of Mr 35,000 was identified by 100% of these dogs, parts of which were E. granulosus specific. Results of this study should allow identification of specific recombinant antigens for routine serodiagnosis of E. granulosus infection in dogs.
University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria, Australia.
R B
RB
Gasser
M W
MW
Lightowlers
M D
MD
Rickard
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004443:Echinococcosis / Q000175:diagnosis / Q000662:veterinary*; D004446:Echinococcus / Q000276:immunology*; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification; D012698:Serologic Tests
D000947:Antigens, Helminth; D011506:Proteins