Genetic susceptibility to mucosal damage leads to bacterial translocation in a murine burn model.
Since genetic factors may be important in host resistance to infections after thermal injury, we screened the susceptibility of three mouse strains (CD-1, Balb/c, and C57/bl) to thermally induced bacterial translocation from the GI tract. Bacteria translocated to the MLNs of Balb/c but not the CD-1 or C57/bl mice receiving 25% body burns. The increased incidence of bacterial translocation in the burned Balb/c mice appeared to be due to a burn-induced gut mucosal injury, since the intestinal mucosa of the Balb/c but not the CD-1 or C57/bl mice was damaged 24 hr after the thermal injury. The mucosal injury appears to be mediated, at least in part, by xanthine oxidase-generated oxygen-free radicals, since inhibition of xanthine oxidase activity with allopurinol, or inactivation of xanthine oxidase activity by a molybdenum-free tungsten diet, prevented the mucosal injury and reduced the extent of bacterial translocation.
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Department of Surgery, LSU Medical Center, Shreveport 71130-3932.
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Deitch
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Specian
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Berg
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D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D001424:Bacterial Infections / Q000150:complications / Q000235:genetics*; D002056:Burns / Q000150:complications* / Q000382:microbiology; D004198:Disease Susceptibility; D007082:Ileum / Q000473:pathology; D007413:Intestinal Mucosa / Q000473:pathology; D008198:Lymph Nodes / Q000382:microbiology*; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000382:microbiology*; D013045:Species Specificity