Charge distribution of plasma IgA in IgA nephropathy.
The spectrotype of plasma IgA in patients with IgA nephropathy was studied by isoelectric focussing. Densitometry of the gels showed a significant increase in the anionic region at isoelectric points (pI) 4.7-5.1 (P = 0.02) and a reduction in the cationic region pI 5.8-6.0 (P = 0.03) in patients (n = 15) compared with controls (n = 8). Measurement of the IgA concentration in eluates of sequential slices of the gels showed that the ratio of anionic-to-cationic IgA, using pI 5.6 as the dividing point, was significantly greater in patients (n = 10) than in controls (n = 10) (P = 0.03). Two different methods of analysis have therefore demonstrated an increased proportion of anionic and decreased proportion of cationic IgA in the plasma of patients with IgA nephropathy.
Renal Unit, United Medical School of Guy's, London, England.
T
T
Harada
P
P
Hobby
M
M
Courteau
J F
JF
Knight
D G
DG
Williams
D000293:Adolescent; D000328:Adult; D005922:Glomerulonephritis, IGA / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A; D007525:Isoelectric Focusing; D008875:Middle Aged
D007070:Immunoglobulin A