Estimation of cholesterol loading of the low-density lipoprotein fraction in diabetic subjects without ultracentrifugation.
We report here a new formula for estimating apolipoprotein (apo) B concentration in the low-density lipoprotein (LDL) fraction from measurements of plasma triglyceride and apoB. ApoB in plasma and in the triglyceride-rich lipoprotein fraction (VLDL, d less than 1.019) and plasma triglyceride were measured in 112 subjects, including 56 diabetics. There was a significant correlation between VLDL-apoB and plasma triglyceride (Y = 0.07X + 1, r = 0.73, P less than 0.001). We calculated LDL-apoB according to this formula: LDL-apoB = total apoB - (0.07 x total triglyceride + 1). We found an excellent relationship between LDL-apoB (total apoB - VLDL-apoB) and calculated LDL-apoB (Y = 1.0X + 1, r = 0.96, P less than 0.001). This new formula will enable us to estimate the apoB concentration in the LDL fraction without ultracentrifugation.
Second Department of Internal Medicine, Kobe University of Medicine, Japan.
G
G
Yoshino
M
M
Iwai
K
K
Matsuba
I
I
Iwatani
M
M
Matsushita
T
T
Kazumi
S
S
Baba
D001055:Apolipoproteins B / Q000097:blood*; D002784:Cholesterol / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood*; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000097:blood; D015228:Hypertriglyceridemia / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D008722:Methods; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D014280:Triglycerides / Q000097:blood*; D014461:Ultracentrifugation / Q000379:methods
D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D011933:Reagent Kits, Diagnostic; D014280:Triglycerides; D002784:Cholesterol