gamma-Aminobutyric acid uptake by rat kidney brush-border membrane vesicles.
Brush-border membrane vesicles (BBMV) from rat kidney cortex possessed two uptake systems for gamma-aminobutyric acid (GABA), a high affinity system (Km = 10.9 microM) and a low affinity system (Km = 1203 microM). Both uptake systems were inhibited by p-hydroxymercuribenzoic acid and ouabain, and by the action of neuraminidase, whereas the GABA analogs nipecotic acid, beta-alanine, 2,4-diaminobutyric acid and 4,5,6,7-tetrahydroisoxazolo-[4,5 c]-pyridin-3-ol had no effect on the GABA uptake activity. The BBMV uptake systems were clearly different from the GABA transport systems present in brain tissue.
Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
H S
HS
Sidhu
J D
JD
Wood
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D009557:Nipecotic Acids / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D009557:Nipecotic Acids; D005680:gamma-Aminobutyric Acid; D009439:Neuraminidase