Identification, by immunoblotting, of the structures carrying Lutheran and para-Lutheran blood group antigens.
Immunoblotting was performed, after sodium dodecyl sulphate polyacrylamide gel electrophoresis of solubilised red cell membranes under non-reducing conditions, with human Lutheran antibodies, anti-Lua, -Lub, -Lu3, -Lu6 and -Lu8, and with human para-Lutheran antibodies, -Lu4, -Lu12 and -Lu17. On antigen-positive red cells all antibodies revealed two components of apparent molecular weight (Mr) 83,000 and 76,000. Anti-Lua and -Lub gave no bands with Lu(a-b+) or Lu(a+b-) cells, respectively. With the exception of anti-Lu17, which reacted normally, and anti-Lu3, which reacted weakly, none of the antibodies showed bands with membranes from Lu(a-b-) cells of the In(Lu) type. Treatment of the red cells with sialidase led to a small reduction in the apparent Mr of the Lutheran glycoproteins which, therefore, appear to contain sialic acid bearing O-linked oligosaccharides.
Medical Research Council Blood Group Unit, London, UK.
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Daniels
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Khalid
D004910:Erythrocyte Membrane / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D007519:Isoantigens / Q000032:analysis*; D008188:Lutheran Blood-Group System / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis; D008970:Molecular Weight
D007519:Isoantigens; D008188:Lutheran Blood-Group System; D008562:Membrane Glycoproteins