Antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations.
The formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside Quil A with an antigen preparation derived from herpes simplex virus type 1 (HSV-1)-infected cell cultures using a zwitterionic detergent is described. The HSV-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against HSV-1 or HSV-2 lethal, systemic challenge infection in animals given a single dose containing 5 micrograms of protein. The HSV-1 iscom preparation also provided significant protection in mice against local reactions following challenge with HSV-1 by skin scarification.
Department of Virology, Medical School, University of Sheffield, U.K.
M
M
Ertürk
R
R
Jennings
D
D
Hockley
C W
CW
Potter
D000276:Adjuvants, Immunologic / Q000276:immunology*; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology*; D003902:Detergents / Q000008:administration & dosage; D005260:Female; D005782:Gels / Q000008:administration & dosage; D006561:Herpes Simplex / Q000276:immunology* / Q000517:prevention & control; D051379:Mice; D008812:Mice, Hairless; D008807:Mice, Inbred BALB C; D009930:Organic Chemicals; D014759:Viral Envelope Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D003902:Detergents; D005782:Gels; D009930:Organic Chemicals; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; D000536:Aluminum Hydroxide; C041772:empigen BB