[Complementary addressed modification of plasmid DNA].
The possibility to accomplish the sequence-specific chemical modification of superhelical DNA with reactive oligonucleotide derivatives was demonstrated. Plasmids containing fragments of the immunoglobulin gene were modified with alkylating derivatives of oligonucleotides complementary to a nucleotide sequence in the immunoglobulin gene. In contrast to the relaxed plasmid DNAs, superhelical DNAs (sigma = -0.1) were found to be attacked by the derivatives at the target nucleotide sequence. The efficiency of the reaction increases with the increase of the plasmids negative superhelicity. It was found also that the denatured derivatives. The sequence-specific modification of plasmid DNAs with the reactive oligonucleotide derivatives can be used for the site-directed mutagenesis and the investigation of the repair processes.
V V
VV
Vlasov
S A
SA
Gaĭdamakov
A A
AA
Nikonova
A S
AS
Levina
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004278:DNA, Superhelical / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics; D051379:Mice; D009841:Oligonucleotides / Q000235:genetics; D010957:Plasmids
D004278:DNA, Superhelical; D007136:Immunoglobulins; D009841:Oligonucleotides; D004247:DNA
Komplementarno adresovannaia modifikatsiia DNK plazmid.