Influence of etoposide and cyclophosphamide on the efficacy of cloxacillin and erythromycin in an experimental staphylococcal infection.
The effect of monocytopenia and granulocytopenia on the outgrowth of Staphylococcus aureus as well as on antibiotic efficacy was studied in an experimental thigh infection in mice. Pretreatment with etoposide reduced monocyte numbers in blood to 14% and those of granulocytes to 54% at the time of infection. Monocytopenia did not affect the proliferation of bacteria in the infected thigh or the reduction of bacterial numbers after treatment with cloxacillin or erythromycin. Pretreatment with cyclophosphamide reduced monocyte numbers to 15% and granulocyte numbers to 3%. This resulted in a marked increase in the number of bacteria at the site of infection and a decrease in the efficacy of antibiotic treatment.
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
W
W
Calame
R
R
van der Waals
H
H
Mattie
R
R
van Furth
D000818:Animals; D003023:Cloxacillin / Q000493:pharmacokinetics / Q000627:therapeutic use*; D003520:Cyclophosphamide / Q000493:pharmacokinetics / Q000494:pharmacology* / Q000633:toxicity; D004347:Drug Interactions; D004917:Erythromycin / Q000493:pharmacokinetics / Q000627:therapeutic use*; D005047:Etoposide / Q000493:pharmacokinetics / Q000494:pharmacology*; D007958:Leukocyte Count; D007970:Leukopenia / Q000139:chemically induced; D051379:Mice; D013203:Staphylococcal Infections / Q000188:drug therapy*
D004917:Erythromycin; D005047:Etoposide; D003520:Cyclophosphamide; D003023:Cloxacillin