title: Reduced DNA damage induced in human melanoma cells by dacarbazine in the presence of deoxyribonucleosides.
abstract: Decarbazine induces DNA lesions in replicating DNA. We show here that deoxyribonucleosides, with the exception of thymidine, in doses of 250 microM or higher, prevent dacarbazine-induced DNA lesions. The DNA lesions that appear in the presence of thymidine can be prevented by aphidicolin, an inhibitor of DNA synthesis. Cytotoxicity analyses confirm that the combination thymidine-dacarbazine prevents cell outgrowth only partially, while other deoxyribonucleosides completely abolish the dacarbazine effect.
authors: Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
authors: U
authors: U
authors: Lönn
authors: S
authors: S
authors: Lönn
authors: U
authors: U
authors: Nylen
authors: G
authors: G
authors: Winblad
mesh_terms: D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects*; D003606:Dacarbazine / Q000037:antagonists & inhibitors*; D003839:Deoxyadenosines / Q000494:pharmacology*; D003841:Deoxycytidine / Q000494:pharmacology*; D003849:Deoxyguanosine / Q000494:pharmacology*; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D013936:Thymidine / Q000494:pharmacology
chemical_list: D004273:DNA, Neoplasm; D003839:Deoxyadenosines; D003841:Deoxycytidine; D003606:Dacarbazine; D003849:Deoxyguanosine; D013936:Thymidine