Keloid fibroblasts in culture: abnormal growth behaviour and altered response to the epidermal growth factor.
Keloid fibroblasts were propagated in culture and their proliferative behaviour and response to the Epidermal Growth Factor (EGF) were studied. Keloid fibroblasts grew at a rate which was approximately one-half that of normal age, sex and race matched control fibroblasts. Keloid fibroblasts were stimulated to grow in the presence of EGF (10 ng/ml), but to a lesser degree than the normal control fibroblasts. Scatchard analysis of the binding data obtained using 125I-labeled EGF revealed no difference in binding affinity or receptor numbers between keloid and normal fibroblasts.
Harper
R A
RA
Connective Tissue Research Institute, University City Science Center, Philadelphia, Pennsylvania 19104.
D000328:Adult; D002455:Cell Division; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007627:Keloid / Q000378:metabolism / Q000473:pathology*; D012867:Skin / Q000166:cytology; D013997:Time Factors
D004815:Epidermal Growth Factor