Isolation and characterization of hypeptin from Pseudomonas sp.
An antibiotic, named hypeptin, was isolated from the culture broth of a strain of Pseudomonas sp. The antibiotic was extracted with butanol and purified by chromatography on a Sephadex LH-20 column. The antibiotic is basic in nature. The dihydrochloride is soluble in aqueous alcohols and positive to ninhydrin and Sakaguchi's reaction. A molecular formula, C44H71N13O15.2HCl was indicated by microanalysis and secondary ion MS. The IR spectrum and acid hydrolysis indicated it to be a peptide antibiotic. The stereochemistries of the amino acids produced by hydrolysis were clarified by HPLC using a chiral column. The antibiotic is active in vitro against a wide variety of anaerobic bacteria and aerobic Gram-positive bacteria.
Shoji
J
J
Shionogi Research Laboratory, Shionogi & Co., Ltd., Osaka, Japan.
Hinoo
H
H
Hattori
T
T
Hirooka
K
K
Kimura
Y
Y
Yoshida
T
T
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006094:Gram-Positive Bacteria / Q000187:drug effects; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D011549:Pseudomonas / Q000378:metabolism
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C061460:hypeptin