Glycogen metabolism during tension generation and maintenance in vascular smooth muscle.
To study the regulation of glycogen utilization in vascular smooth muscle, we measured the content of glycogen and glucose 6-phosphate and the activity of the glycogen phosphorylase and glycogen debrancher enzymes in porcine carotid artery. During active contraction, the rates of glycogen phosphorylase and glycogenolysis were as high as expected. Despite this, glycogen content did not decrease to less than approximately 50% of control levels even after sustained contractions. The activity of glycogen debrancher enzyme was found to be limiting glycogen utilization at this point. Although glycogenolysis is closely coordinated with increases in oxidative metabolism concomitant with active contraction, the maximal level of tension obtained after stimulation was not substantially reduced under conditions where glycogen debrancher enzyme was limiting glycogen utilization. On the other hand, the rate of tension generation was increased in these tissues. Thus glycogen utilization is not necessary for maximal force generation per se, but may influence other muscle contractile properties. Finally, during steady-state tension maintenance, glycogen utilization is likely to be regulated by the intracellular concentrations of metabolic intermediates (glucose, glucose 6-phosphate), as it is in skeletal muscle.
Lynch
R M
RM
Department of Physiology and Biophysics, College of Medicine, University of Cincinnati, Ohio 45267-0576.
Kuettner
C P
CP
Paul
R J
RJ
D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism / Q000502:physiology; D005947:Glucose / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism*; D006004:Glycogen Debranching Enzyme System / Q000378:metabolism; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D007700:Kinetics; D009119:Muscle Contraction; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000502:physiology*; D006005:Phosphorylases / Q000378:metabolism; D013552:Swine
D006004:Glycogen Debranching Enzyme System; D006003:Glycogen; D006005:Phosphorylases; C030563:amylo-1,6-glucosidase; D005947:Glucose