Antinociceptive effect of intrathecally administered antiserum against calcitonin gene-related peptide on thermal and mechanical noxious stimuli in experimental hyperalgesic rats.
We compared the effects of intrathecal administration of antiserum against calcitonin gene-related peptide (CGRP) between thermo- and mechano-nociceptive responses, using experimental hyperalgesic rats. An intrathecal administration of anti-CGRP antiserum, but not antiserum absorbed by synthetic CGRP, normalized either adjuvant- or carrageenin-induced hyperalgesia both in the paw radiant heat and the paw pressure tests, with little effect on non-hyperalgesic paws. These results suggest that endogenous CGRP, probably present in primary afferents, promotes both thermo- and mechano-nociceptive transmission in the spinal dorsal horn, at least in the hyperalgesic states with inflammations.
Kawamura
M
M
Department of Pharmacology, Faculty of Pharmaceutical Science, Kyoto University, Japan.
Kuraishi
Y
Y
Minami
M
M
Satoh
M
M
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000502:physiology*; D006930:Hyperalgesia / Q000378:metabolism*; D006941:Hyperesthesia / Q000378:metabolism*; D007106:Immune Sera / Q000494:pharmacology*; D007278:Injections, Spinal; D008297:Male; D010146:Pain / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007106:Immune Sera; D015740:Calcitonin Gene-Related Peptide