title: Bovine peripheral blood leukocyte population dynamics following treatment with recombinant bovine interferon-alpha I1.
abstract: The in vitro functional activity of bovine peripheral blood lymphocytes and the population dynamics of the major peripheral blood leukocyte subpopulations were investigated following the administration of recombinant bovine interferon-alpha I1 (rBoIFN-alpha I1). The intramuscular injection of rBoIFN-alpha I1 induced a 24-h lymphopenia characterized by a decrease in both circulating T and non-T/non-B lymphocytes. An increased CD4/CD8 ratio indicated that there was a relatively greater depletion of the CD8 equivalent subpopulation of T lymphocytes. These changes in lymphocyte trafficking were observed in the absence of a cortisolemia. Coincidental with the lymphopenia, there was a marked decrease in the amplitude of mitogen-induced lymphocyte proliferation responses that probably was caused by a numerical deficit rather than functional deficit in the responder T cells.
authors: Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada.
authors: P J
authors: PJ
authors: Griebel
authors: H
authors: H
authors: Bielefeldt Ohmann
authors: M
authors: M
authors: Campos
authors: L
authors: L
authors: Qualtiere
authors: W C
authors: WC
authors: Davis
authors: M J
authors: MJ
authors: Lawman
authors: L A
authors: LA
authors: Babiuk
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D002417:Cattle; D002469:Cell Separation; D005434:Flow Cytometry; D006854:Hydrocortisone / Q000032:analysis; D007273:Injections, Intramuscular; D007370:Interferon Type I / Q000494:pharmacology*; D008214:Lymphocytes / Q000145:classification* / Q000187:drug effects; D008231:Lymphopenia / Q000209:etiology; D011994:Recombinant Proteins; D013997:Time Factors
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D007370:Interferon Type I; D011994:Recombinant Proteins; D006854:Hydrocortisone