DNA sequence determination of gamma-radiation-induced mutations of the hamster aprt locus.
From a collection of 85 independent gamma-radiation hamster aprt- mutants, 27 having no major structural alterations were analysed at the nucleotide level by using the polymerase chain reaction to amplify mutant exons and then directly sequencing the double-stranded products. The majority of these mutations were simple base substitutions of all types, particularly transversions (11/27). Frameshifts and small deletions were also induced. The 'spectrum' of mutations produced by gamma-radiation was not significantly different from that occurring spontaneously at this locus. Differences with respect to the target and structure of frameshifts and small deletions occurring in the two collections were apparent.
Miles
C
C
Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire, Great Britain.
Meuth
M
M
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D004247:DNA / Q000528:radiation effects*; D004252:DNA Mutational Analysis; D004259:DNA-Directed DNA Polymerase; D005720:Gamma Rays; D008969:Molecular Sequence Data; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011839:Radiation, Ionizing; D019914:Taq Polymerase
D004247:DNA; D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase