title: Endotoxemia elicits increased circulating beta 2-IFN/IL-6 in man.
abstract: beta 2-IFN/hepatocyte stimulating factor/IL-6 is a cytokine secreted by monocytes, fibroblasts, and endothelial cells in cell culture that possesses diverse biologic activity including the stimulation of acute phase plasma protein synthesis and immunomodulation. The circulating levels of this cytokine in man in response to bacterial LPS (endotoxin) were studied. A single i.v. bolus of endotoxin (20 U/kg) produced a monophasic rise in circulating immunoreactive IFN-beta 2/IL-6 and IFN-beta 2/IL-6 bioactivity (hepatocyte stimulation and B cell differentiation assays) peaking 2 to 4 h after the endotoxin challenge. Peak IFN-beta 2/IL-6 levels ranged from 4.1 to 27.5 ng/ml. Associated with this was a rise in circulating C-reactive protein levels detected 20 h after the endotoxin bolus. Thus, IFN-beta 2/IL-6 is likely one of the endogenous mediators which is triggered in man during bacterial infection and likely participates in the metabolic and immune responses of the infected host.
authors: Laboratory of Surgical Metabolism, New York Hospital-Cornell Medical Center 10021.
authors: Y
authors: Y
authors: Fong
authors: L L
authors: LL
authors: Moldawer
authors: M
authors: M
authors: Marano
authors: H
authors: H
authors: Wei
authors: S B
authors: SB
authors: Tatter
authors: R H
authors: RH
authors: Clarick
authors: U
authors: U
authors: Santhanam
authors: D
authors: D
authors: Sherris
authors: L T
authors: LT
authors: May
authors: P B
authors: PB
authors: Sehgal
mesh_terms: D000328:Adult; D002097:C-Reactive Protein / Q000032:analysis; D004731:Endotoxins / Q000008:administration & dosage; D006801:Humans; D015151:Immunoblotting; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis / Q000097:blood* / Q000302:isolation & purification; D008099:Liver / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D012772:Shock, Septic / Q000097:blood* / Q000209:etiology
chemical_list: D004731:Endotoxins; D015850:Interleukin-6; D007378:Interleukins; D002097:C-Reactive Protein