A specific defect in the proliferative capacity of B cells from old mice stimulated with autoreactive T cells.
B lymphocytes from aged mice were found to be defective in their ability to proliferate in response to stimulation with an autoreactive T cell clone D1.4. The differentiative response leading to antibody secretion was also impaired in the auto D1.4 T cell-stimulated B cells from old mice in comparison to similarly stimulated B cells from young mice. The B cells from old mice were competent in activating the autoreactive T cells such that the T cells were induced to proliferate. The B cell defect appears to be restricted to a certain phase of B cell activation, since old mouse B cells responded to the auto D1.4 T cells by increasing cell surface Ia as well as size, but failed to incorporate tritiated thymidine. The responsiveness to interleukin-4 was found to be similar between B cells from young and old mice. It appeared that the B cells from old mice are specifically defective in progressing from the G0 phase of cell cycle into the S phase when stimulated with the auto D1.4 T cells.
Nagarkatti
P S
PS
Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Seth
A
A
Nagarkatti
M
M
Muthusamy
N
N
Rychlik
B
B
Subbarao
B
B
D000375:Aging; D001324:Autoantigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002453:Cell Cycle; D002454:Cell Differentiation; D002478:Cells, Cultured; D002999:Clone Cells; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007075:Immunoglobulin M / Q000378:metabolism; D066298:In Vitro Techniques; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D013601:T-Lymphocytes / Q000276:immunology*
D001324:Autoantigens; D000949:Histocompatibility Antigens Class II; D007075:Immunoglobulin M; D007378:Interleukins; D015847:Interleukin-4