Purification of human alpha 2-plasmin inhibitor using monoclonal antibody column chromatography.
alpha 2-Plasmin inhibitor (alpha 2PI) was purified directly from human plasma by using a monoclonal antibody affinity column, which recognizes the reactive site of alpha 2PI. alpha 2PI was eluted from the column under mild conditions with 50% v/v ethyleneglycol containing 0.05% Tween 80 in phosphate-buffered saline, pH 7.4. The yield was around 50% and the specific activity of the purified protein was the same as that of the best product of preparation conventionally purified alpha 2PI.
Sumi
Y
Y
Biotechnology Research Laboratory, Teijin Limited, Hino, Tokyo.
Koike
Y
Y
Ichikawa
Y
Y
Aoki
N
N
D000911:Antibodies, Monoclonal; D000933:Antifibrinolytic Agents; D001665:Binding Sites; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007120:Immunochemistry; D000979:alpha-2-Antiplasmin / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000933:Antifibrinolytic Agents; D000979:alpha-2-Antiplasmin