Transforming growth factor beta specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes.
The addition of TGF-beta to cultures of LPS-stimulated murine B cells causes a approximately 10-fold enhancement of IgA production, yet causes a 10-fold decrease in total Ig production. IL-2 and, to a lesser extent, IL-5 synergize with TGF-beta to further enhance IgA production and partially reverse the inhibition of total Ig production. IgA constitutes only approximately 0.1% of total Ig in LPS-stimulated cultures, but that percentage rises to 15-25% in cultures to which TGF-beta and IL-2 are added. TGF-beta induces a substantial increase in IgA production from sIgA- B cells but inhibits IgA production by sIgA+ cells. This finding suggests that TGF-beta acts as an isotype-specific switch factor for IgA.
Coffman
R L
RL
Department of Immunology, DNAX Research Institute, Palo Alto, California 94304.
Lebman
D A
DA
Shrader
B
B
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000494:pharmacology; D015848:Interleukin-5; D007378:Interleukins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D015291:Transforming Growth Factors / Q000494:pharmacology*
D007070:Immunoglobulin A; D007376:Interleukin-2; D015848:Interleukin-5; D007378:Interleukins; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D015291:Transforming Growth Factors