Detection of major histocompatibility complex class I-restricted, HIV-specific cytotoxic T lymphocytes in the blood of infected hemophiliacs.
Major histocompatibility (MHC)-restricted, human immunodeficiency virus type one (HIV-1)-specific, cytotoxic T lymphocytes (CTLs) were detected in the peripheral blood mononuclear cells (PBMCs) of HIV-1-infected individuals. Using a system of autologous B and T lymphoblastoid cell lines infected with recombinant vaccinia vectors (VVs) expressing HIV-1 gene products, we were able to detect HIV-1-specific cytolytic responses in the PBMCs of 88% of HIV-1-seropositive hemophiliac patients in the absence of in vitro stimulation. These cytolytic responses were directed against both HIV-1 envelope and gag gene products. The responses were resistant to natural killer (NK) cell depletion and were inhibited by monoclonal antibodies (MoAbs) to the T cell receptor, CD8 surface antigens, and MHC class I antigens, suggesting a classical MHC class I restricted, virus-specific CTL response.
Koup
R A
RA
Department of Pediatrics, University of Massachusetts Medical School, Worcester.
Sullivan
J L
JL
Levine
P H
PH
Brettler
D
D
Mahr
A
A
Mazzara
G
G
McKenzie
S
S
Panicali
D
D
D000911:Antibodies, Monoclonal / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive; D002461:Cell Line, Transformed; D003601:Cytotoxicity Tests, Immunologic; D005822:Genetic Vectors; D006679:HIV Seropositivity / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006467:Hemophilia A / Q000097:blood* / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007694:Killer Cells, Natural / Q000276:immunology; D011446:Prospective Studies; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification* / Q000276:immunology
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I