Alkyl and aryl carcinogen adducts detected in human peripheral lung.
Human peripheral lung tissue samples were obtained at autopsy from 17 individuals of known occupational and smoking histories. A spectrum of different carcinogen-DNA adducts was detected using a variety of sensitive techniques. High-pressure liquid chromatography-linked synchronous fluorescent spectrophotometry and an ultrasensitive enzyme radioimmunoassay detected adducts derived from benzo[a]pyrene diol epoxide and other apparent polycyclic aromatic hydrocarbons. An amplified enzyme-linked immunosorbent assay demonstrated the presence of 4-aminobiphenyl-DNA adducts in many of these samples. A number of these specimens also contained O6-alkyldeoxyguanosine as measured by 32P-postlabeling techniques. Thus this pilot study indicates not only that human lung contains a spectrum of carcinogen-DNA adducts, but also that a full scale molecular dosimetry study of human exposure to both aryl and alkyl chemical carcinogens is warranted.
Wilson
V L
VL
Division of Cancer Etiology, National Cancer Institute, Bethesda, MD 20892.
Weston
A
A
Manchester
D K
DK
Trivers
G E
GE
Roberts
D W
DW
Kadlubar
F F
FF
Wild
C P
CP
Montesano
R
R
Willey
J C
JC
Mann
D L
DL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000478:Alkylation; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis*; D004249:DNA Damage; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D008168:Lung / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D012907:Smoking
D002273:Carcinogens; D004247:DNA