Surfactant secretion and clearance in the newborn.
Pregnant rabbits (30 days) were injected intravenously with [3H]choline 8 h before delivery. The fetuses were delivered, and lung lavage and lamellar body phospholipids (PL) were analyzed. Some newborns also received radioactively labeled surfactant intratracheally on delivery and were permitted to breathe. With time, intratracheal label decreased in lavage and appeared in the lamellar body fraction, and intravenous label accumulated in both pools. Using a tracer analysis for non-steady state, we calculated surfactant secretion and clearance rates for the newborn period. Before birth, both rates rose slightly from 1.8 micrograms PL.g body wt-1.h-1 at 6 h before birth to 7.3 at birth. Immediately after birth, secretion rate rose to 37.7 micrograms PL.g body wt-1.h-1. Between 1.5 and 2 h after birth it fell to a minimum of 1.8 micrograms PL.g body wt-1.h-1 and then rose slowly to 6.0 at 12 h. After birth, clearance rate increased less than secretion rate (maximum 24.7 micrograms PL.g body wt-1.h-1 shortly after birth) then followed the same pattern but did not balance secretion rate in the 1st day.
Stevens
P A
PA
Cardiovascular Research Institute, University of California, San Francisco 94143.
Wright
J R
JR
Clements
J A
JA
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000196:embryology / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D010743:Phospholipids / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011817:Rabbits; D014316:Tritium
D010743:Phospholipids; D011663:Pulmonary Surfactants; D014316:Tritium; D007770:L-Lactate Dehydrogenase; D002794:Choline