Differentiation of intestinal epithelial cell line (IEC-18) by an acid extract of rat small intestine.
A factor which may induce differentiation of intestinal epithelial cell lines in vitro was found in an acid extract of adult rat small intestine. The addition of a partially purified acetic acid extract of rat small intestine to IEC-18 cell culture dishes increased sucrase activity within 48 h. Thymidine incorporation markedly decreased within 24 h. Significant development of microvilli-like structures was observed on the acid extract-treated IEC-18 cells, compared with controls. This activity of rat acid extract was heat-stable and the apparent molecular weight of the factor was 400-800. These findings suggested that the factor may be related to the epithelial differentiation of rat small intestinal crypt cells.
Shintani
T
T
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Fushiki
T
T
Fukuoka
S
S
Takahashi-Iwanaga
H
H
Sugimoto
E
E
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004261:DNA Replication / Q000187:drug effects; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D007421:Intestine, Small / Q000502:physiology*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D009130:Muscle, Smooth / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*
D014020:Tissue Extracts