Bovine peripheral blood leukocyte population dynamics following treatment with recombinant bovine interferon-alpha I1.
The in vitro functional activity of bovine peripheral blood lymphocytes and the population dynamics of the major peripheral blood leukocyte subpopulations were investigated following the administration of recombinant bovine interferon-alpha I1 (rBoIFN-alpha I1). The intramuscular injection of rBoIFN-alpha I1 induced a 24-h lymphopenia characterized by a decrease in both circulating T and non-T/non-B lymphocytes. An increased CD4/CD8 ratio indicated that there was a relatively greater depletion of the CD8 equivalent subpopulation of T lymphocytes. These changes in lymphocyte trafficking were observed in the absence of a cortisolemia. Coincidental with the lymphopenia, there was a marked decrease in the amplitude of mitogen-induced lymphocyte proliferation responses that probably was caused by a numerical deficit rather than functional deficit in the responder T cells.
Griebel
P J
PJ
Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada.
Bielefeldt Ohmann
H
H
Campos
M
M
Qualtiere
L
L
Davis
W C
WC
Lawman
M J
MJ
Babiuk
L A
LA
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D002417:Cattle; D002469:Cell Separation; D005434:Flow Cytometry; D006854:Hydrocortisone / Q000032:analysis; D007273:Injections, Intramuscular; D007370:Interferon Type I / Q000494:pharmacology*; D008214:Lymphocytes / Q000145:classification* / Q000187:drug effects; D008231:Lymphopenia / Q000209:etiology; D011994:Recombinant Proteins; D013997:Time Factors
D000945:Antigens, Differentiation, T-Lymphocyte; D007370:Interferon Type I; D011994:Recombinant Proteins; D006854:Hydrocortisone