Calcitonin gene-related peptide enhances spontaneous acetylcholine release from the rat motor nerve terminal.
The effect of synthetic rat calcitonin gene-related peptide (rCGRP) on neuromuscular transmission was examined in a superfused rat phrenic nerve-diaphragm preparation using an intracellular microelectrode technique. The superfusion with rCGRP (10(-8) to 2 x 10(-7) M) caused significant increases in the frequency of miniature endplate potentials (MEPPs). It, however, had no effect on the resting membrane potential, amplitude of MEPPs, or acetylcholine quantum size and content. Enhancement of spontaneous acetylcholine release from the motor nerve terminal by rCGRP was demonstrated.
Jinnai
K
K
Department of Medicine, Kobe University School of Medicine, Japan.
Chihara
K
K
Kanda
F
F
Tada
K
K
Fujita
T
T
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D003964:Diaphragm / Q000294:innervation; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D009045:Motor Endplate / Q000187:drug effects / Q000502:physiology*; D009469:Neuromuscular Junction / Q000502:physiology*; D009479:Neuropeptides / Q000494:pharmacology*; D010791:Phrenic Nerve / Q000502:physiology; D051381:Rats
D009479:Neuropeptides; D015740:Calcitonin Gene-Related Peptide; D000109:Acetylcholine