Interleukin 7 production and function in stromal cell-dependent B cell development.
The role of IL-7 in the stromal cell-dependent B cell development was investigated using two stromal cell clones, ST2 and PA6; the former supports B lymphopoiesis while the latter can not. We demonstrate here that: (a) the ability of the stromal cell clone to produce IL-7 correlates well with the stromal cell activity to support B lymphopoiesis; (b) IL-7 production by ST2 is inducible rather than constitutive; (c) the IL-7-dependent B cell itself is a potent inducer of IL-7 production by ST2; (d) addition of rIL-7 to the PA6 layer renders this in vitro environment B lymphopoietic; and (e) the differentiation from early B progenitor to pre-B cell requires both IL-7 and other stromal cell molecule(s) yet to be identified.
Sudo
T
T
Biomaterial Research Institute Co. Ltd., Yokohama, Japan.
Ito
M
M
Ogawa
Y
Y
Iizuka
M
M
Kodama
H
H
Kunisada
T
T
Hayashi
S
S
Ogawa
M
M
Sakai
K
K
Nishikawa
S
S
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D015342:DNA Probes; D005796:Genes; D015851:Interleukin-7; D007378:Interleukins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000302:isolation & purification; D015183:Restriction Mapping; D014158:Transcription, Genetic
D015342:DNA Probes; D015851:Interleukin-7; D007378:Interleukins; D012333:RNA, Messenger; D011994:Recombinant Proteins