A light and electron microscopic study of calcitonin gene-related peptide in the rat caudate putamen.
Calcitonin gene-related peptide (CGRP)-like immunoreactive (CGRP-IR) structures have been studied in the rat caudate putamen using avidin-biotin peroxidase immunohistochemistry. Immunoreactivity was found in the axons of this nucleus but not in the perikarya. CGRP-IR fibers were most densely concentrated along the ventral border and in the caudal portion of the rat caudate putamen. CGRP-IR fibers were sparsely distributed throughout the rest of this nucleus. Almost all immunoreactive boutons which contained small clear vesicles had formed asymmetrical synapses. Postsynaptic targets included dendritic spines and shafts. Asymmetrical synapses in the caudate putamen are supposed to be extrinsic in origin. These observations, together with the results of other investigations, suggest that CGRP-IR boutons form synapses with spiny striatal neurons, which, most likely, are medium-sized spiny projecting neurons. Moreover, evidence indicates that these boutons are of extrinsic origin.
Okayama
T
T
Third Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Kubota
Y
Y
Kito
S
S
Funaki
H
H
Shimada
S
S
Takagi
H
H
Inagaki
S
S
D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D003342:Corpus Striatum / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D009479:Neuropeptides; D015740:Calcitonin Gene-Related Peptide