Kinetic assay of cytotoxic T lymphocyte clones. II. Comparison of primary and secondary responses and lectin-dependent cytotoxicity.
A kinetic assay for cytotoxic T lymphocyte (CTL) clones expanded briefly in vitro was used to compare CTL clones developing from the most frequent precursor population in naive spleens, among splenocytes primed in vitro, and among cells mediating lectin-dependent cellular cytotoxicity. Two kinetic parameters, K0 and n, were compared; K0 is the target cell concentration required for half-maximal velocity of killing, and n is the exponent of the target concentration in the Hill equation used for data analysis. The variance and frequency distribution of these parameters, rather than their numerical value, predicted differences in the clonal composition of distinct lymphoid populations assayed independently.
Jenski
L J
LJ
Department of Biology, Indiana University-Purdue University, Indianapolis 46223.
D000818:Animals; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D010835:Phytohemagglutinins