A pregnancy-specific beta 1-glycoprotein, a CEA gene family member, expressed in a human promyelocytic leukemia cell line, HL-60: structures of protein, mRNA and gene.
Both genomic and cDNA clones encoding a precursor for a pregnancy-specific beta 1-glycoprotein (PS beta G) belonging to the CEA family, expressed in a human promyelocytic leukemia cell line, HL-60, have been isolated and the entire primary structure of the precursor is deduced. The 335-AA precursor has a 34-AA signal peptide followed by domains of N, IIA, IIB and C, which are encoded by separate exons. The genomic sequence contains extra exons IA and IB between exons N and IIA. Apparently, exon IA is excluded from the mRNA by alternative splicing while IB is a pseudo-exon having a stop codon formed by a deletion of dinucleotide in the middle of the sequence. This provides another mechanism to render exon IB abortive and is different from that we reported for another PS beta G (Biochem. Biophys. Res. Comm. (1988) 156, 68-77).
Oikawa
S
S
Suntory Institute for Biomedical Research, Osaka, Japan.
Inuzuka
C
C
Kuroki
M
M
Matsuoka
Y
Y
Kosaki
G
G
Nakazato
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006031:Glycosylation; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D008969:Molecular Sequence Data; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000235:genetics*; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics*; D014407:Tumor Cells, Cultured
D002272:Carcinoembryonic Antigen; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins; D012333:RNA, Messenger; D004247:DNA