Characterization and expression of a spliced leader RNA in the parasitic nematode Ascaris lumbricoides var. suum.
The parasitic nematode Ascaris spp. contains a 22-nucleotide spliced-leader (SL) sequence identical to the trans-SL previously described in Caenorhabditis elegans and other nematodes. The SL comprises the first 22 nucleotides of a approximately 110-base RNA and is transcribed by RNA polymerase II. The SL RNA contains a trimethylguanosine cap and a consensus Sm binding site. Furthermore, the Ascaris SL RNA has the potential to adopt a secondary structure which is nearly identical to potential secondary structures of similar SL RNAs in C. elegans and Brugia malayi.
Nilsen
T W
TW
Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Shambaugh
J
J
Denker
J
J
Chubb
G
G
Faser
C
C
Putnam
L
L
Bennett
K
K
D000818:Animals; D001200:Ascaris / Q000196:embryology / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D006151:Guanosine / Q000032:analysis / Q000031:analogs & derivatives; D008969:Molecular Sequence Data; D009348:Nematoda / Q000196:embryology / Q000235:genetics; D009690:Nucleic Acid Conformation; D012315:RNA Caps; D012319:RNA Polymerase II / Q000378:metabolism; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D012315:RNA Caps; D012333:RNA, Messenger; D006151:Guanosine; C017778:N(2),N(2),7-trimethylguanosine; D012319:RNA Polymerase II