Cell-mediated immunity to chemically xenogenized tumors--IV. Production of lymphokine activity by, and in response to, highly immunogenic cells.
To determine whether a novel pattern of lymphokine production might be involved in the superior immunogenicity of chemically xenogenized tumors over that of parental cells, we tested a panel of murine tumors xenogenized by DTIC for production of soluble factors with lymphokine-like activity and induction of lymphokine release from naïve or specifically sensitized lymphocytes. In the L5178Y tumor system, a majority of xenogenized but not parental clones produced an IL-1-like factor, and this was associated, as a rule, with class II antigen expression and antigen-presenting ability. However, no such properties were exhibited by the xenogenized variants of P815 and L1210Ha cells, which nevertheless occasionally expressed other lymphokine (GM-CSF, IL-3) activities. On examining the ability of xenogenized and parental tumors to cause release of IL-1, IL-2, IL-3, IFN-gamma, TNF/LT and GM-CSF from T-cells, we found, as a rule, an increased lymphokine production when lymphocytes primed in vivo to a xenogenized tumor were restimulated in vitro with the same or parental cells.
Romani
L
L
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Puccetti
P
P
Grohmann
U
U
Cenci
E
E
Mage
M G
MG
Fioretti
M C
MC
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D003606:Dacarbazine / Q000494:pharmacology*; D005260:Female; D006649:Histocompatibility Antigens / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects; D066298:In Vitro Techniques; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D009153:Mutagens / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000951:Antigens, Neoplasm; D006649:Histocompatibility Antigens; D008222:Lymphokines; D009153:Mutagens; C029237:tumor-associated transplantation antigen; D003606:Dacarbazine