Establishment of a chicken X chicken hybridoma secreting specific antibody.
Chicken B lymphoblastoid cell mutants lacking thymidine kinase (TK) were selected from the parental cell line treated with ethyl methanesulfonate. To obtain chicken hybridomas secreting immunoglobulin against the defined antigen, a TK- mutant was fused with inactivated Newcastle disease virus (NDV)-immunized chicken spleen cells using polyethyleneglycol. Finally, one hybridoma secreting IgM specific for NDV was established.
Nishinaka
S
S
Department of Microbiology and Hygiene, Faculty of Applied Biological Sciences, Hiroshima University, Japan.
Matsuda
H
H
Murata
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000378:metabolism; D002459:Cell Fusion; D002645:Chickens; D006825:Hybridomas / Q000378:metabolism*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D009522:Newcastle disease virus / Q000276:immunology; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007075:Immunoglobulin M