Members of the human glyceraldehyde-3-phosphate dehydrogenase-related gene family map to dispersed chromosomal locations.
Several highly homologous glyceraldehyde-3-phosphate dehydrogenase (GAPD)-related sequences have been identified previously in human DNA by Southern blot analysis. Protein studies have identified only a single expressed locus for this major glycolytic enzyme, and this maps to chromosome 12p13. Sequence analysis of a GAPD muscle cDNA clone and a GAPD-related clone retrieved from an X-chromosome recombinant library showed that the latter was a processed pseudogene that maps to Xp11-p21. In this study, we have determined the chromosomal locations of several of the additional GAPD-related human sequences using a short 3' end sequence from the cDNA to probe DNA from a series of human-rodent somatic cell hybrids on Southern blots. Eight HindIII GAPD-related sequences detected at high stringency have been mapped to 6 different chromosomes. Several of the additional sequences detected at more moderate stringency have been localized to a further 10 chromosomal sites. Together, these sites constitute the known expressed locus, the known X-linked pseudogene, and 15 GAPD-like loci.
Benham
F J
FJ
Division of Medical and Molecular Genetics, United Medical School of Guy's Hospital, London, United Kingdom.
Povey
S
S
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002881:Chromosomes, Human, Pair 12; D002889:Chromosomes, Human, Pair 2; D002894:Chromosomes, Human, Pair 4; D002896:Chromosomes, Human, Pair 6; D006224:Cricetinae; D015342:DNA Probes; D015247:Deoxyribonuclease HindIII; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D006824:Hybridization, Genetic; D066298:In Vitro Techniques; D051379:Mice; D051381:Rats
D015342:DNA Probes; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D015247:Deoxyribonuclease HindIII