[A study on complement activation in cardiopulmonary bypass--experimental and clinical evaluations].
Complement activation in patients undergoing cardiopulmonary bypass (CPB) was studied, with particular reference to anaphylatoxins. Complement levels were compared between a bubble oxygenator group (BOG) and a membrane oxygenator group (MOG). The influence of primed homologous blood on complement activation was also evaluated. C4a levels gradually increased during CPB in the BOG, whereas in the MOG they changed little. There was a marked initial rise of C4a levels in the BOG of high H/A (primed homologous vs. circulating autologous blood volume ratio). C3a levels increased more steeply in the BOG than in the MOG. In general C5a levels remained low in both groups, but were slightly higher in the BOG than in the MOG. Complement levels were determined in monkeys (Macaca fascicularis) undergoing continuous intraaortic infusion of 1) autologous blood extracorporeally bubbled with O2, 2) blood contacted with nylon, or 3) O2 microbubbles. The results indicated 1) elevation of C4a and C3a in the bubbled blood group, 2) elevation of C3a alone in the nylon group, and 3) no elevation of either anaphylatoxin. It was thus inferred that 1) during CPB, the complement might be rather dominantly activated via the classical pathway in the BOG, and via the alternative pathway in the MOG, 2) higher anaphylatoxin levels in the BOG than in the MOG might be related to mode and grade of blood trauma including plasma denaturation, and 3) the H/A ratio might be a factor able to modify complement activation.
Yamasaki
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D000293:Adolescent; D000328:Adult; D000368:Aged; D000706:Anaphylatoxins / Q000378:metabolism; D000818:Animals; D002315:Cardiopulmonary Bypass; D002648:Child; D002675:Child, Preschool; D003167:Complement Activation; D003181:Complement C4 / Q000378:metabolism; D015934:Complement C4a; D003165:Complement System Proteins / Q000378:metabolism; D006801:Humans; D008252:Macaca fascicularis; D008297:Male; D008875:Middle Aged; D010107:Oxygenators, Membrane
D000706:Anaphylatoxins; D003181:Complement C4; D015934:Complement C4a; D003165:Complement System Proteins