Chemiluminescent labelled streptavidin (STAV) as a universal marker in steroid and peptide immunoassays.
The tetrameric structure of streptavidin and its exceptionally strong affinity to biotin (Ka = 10(15)M 1) can be exploited to achieve an amplification of the signal in immunoassays. In the approach described here streptavidin (STAV) labelled with aminobutylethyl-isoluminol (ABEI) served as a universal marker in immunoassays for both haptens and big antigens. The advantageous features of streptavidin can be applied to any immunoassay using biotinylated antibodies as the primary probe. In two-site immunometric assays for larger antigens the liquid phase 'tracer' antibody is biotinylated. In hapten assays the solid phase antigen technique (Wood et al., 1982) is employed, in which sample-antigen and solid phase-antigen compete for a biotinylated antibody. In this paper we demonstrate the use of STAV-ABEI as a universal chemiluminescent label in steroid assays and in an immunometric assay using human growth hormone (hGH) as an example.
Strasburger
C J
CJ
Klinik für Innere Medizin, Medizinische Universität zu Lübeck, FRG.
Kohen
F
F
D000941:Antigens / Q000032:analysis; D001426:Bacterial Proteins; D001710:Biotin; D006241:Haptens / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives; D010455:Peptides / Q000032:analysis; D013256:Steroids / Q000032:analysis; D019809:Streptavidin
D000941:Antigens; D001426:Bacterial Proteins; D006241:Haptens; D010455:Peptides; D013256:Steroids; D008165:Luminol; C031912:6-(N-(4-aminobutyl)-N-ethyl)amino-2,3-dihydrophthalazine-1,4-dione; D001710:Biotin; D019809:Streptavidin