In vivo and in vitro 31P-NMR spectroscopy of rat liver treated with halocarbons.
Both in vivo and in vitro 31P-NMR spectroscopy were used to demonstrate metabolic changes in rat liver as a function of time after exposure to either carbon tetrachloride (CCl4) or bromotrichloromethane (BrCCl3). The inorganic phosphate resonance, measured in vivo, moves upfield, which is associated with a decrease in cytosolic pH over a 12 or 20 h period (for BrCCl3 or CCl4, respectively). Intoxication by CCl4 or BrCCl3 causes an intracellular acidosis to pH 7.05 or 6.82 (+/- 0.05), respectively. Also, it has been found that halocarbon exposure increases the amounts of phosphomonoesters (PME) detected. High resolution in vitro 31P-NMR spectroscopy studies of perchloric acid extracts of CCl4-treated rat livers indicated a significant increase in the height of the phosphocholine resonance in the PME region 4-5 h after CCl4 exposure.
Towner
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RA
Guelph-Waterloo Centre for Graduate Work in Chemistry, University of Guelph, Canada.
Brauer
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Janzen
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Ling
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D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001975:Bromotrichloromethane / Q000633:toxicity*; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D002725:Chloroform / Q000031:analogs & derivatives*; D005609:Free Radicals; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D000227:Adenine Nucleotides; D005609:Free Radicals; D010710:Phosphates; D010758:Phosphorus; D002725:Chloroform; D001975:Bromotrichloromethane