Characterization of protective T cells in the acquired response to Leishmania donovani in genetically determined cure (H-2b) and noncure (H-2d) mouse strains.
The response to reinfection with Ethiopian Leishmania donovani was evaluated in genetically determined noncure (H-2d) B10.D2 mice that are able to resolve infection due to sublethal irradiation pretreatment after inoculation with a low parasite dose and in C57BL/10 mice that demonstrate the genetically determined cure (H-2b) response to L. donovani. It was found that after resolution of primary infection, C57BL/10 (cure) mice and sublethally irradiated B10.D2 (noncure) mice were resistant to rechallenge with L. donovani. Noncure mice inoculated with a low dose of amastigotes were not, however, solidly immune to reinfection. Adoptive-cell transfer experiments were then done to determine the T-cell subset that was associated with resistance to reinfection, and thus the development of immunity, in sublethally irradiated B10.D2 noncure mice and in C57BL/10 cure mice. T-cell-enriched preparations from spleens of immune donors were treated with subset-specific antibodies and complement prior to adoptive transfer in unprimed recipients. The results of the adoptive transfer experiments provide evidence that the genetically determined cure (H-2b) response in C57BL/10 mice and the cure response in genetically determined noncure (H-2d) B10.D2 mice brought about by sublethal irradiation pretreatment are mediated primarily by an L3T4+ Lyt-2- T cell.
Ulczak
O M
OM
Montreal General Hospital Research Institute, Quebec, Canada.
Ghadirian
E
E
Skamene
E
E
Blackwell
J M
JM
Kongshavn
P A
PA
D000818:Animals; D005260:Female; D006183:H-2 Antigens / Q000235:genetics*; D007110:Immunity, Active; D007111:Immunity, Cellular; D007113:Immunity, Innate; D007116:Immunization, Passive; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012008:Recurrence; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation
D006183:H-2 Antigens