Mutants of luminous bacteria selected for bioluminescent toxicity tests.
Mutants of the luminescent bacterial strain NRRL B-11177 were isolated with pleiotropic hypersensitivity towards hydrophobic antimicrobial agents. SDS-PAGE analyses of outer membrane proteins and lipopolysaccharides revealed that the outer membrane structure of the ahs-mutants was altered. QSAR analysis showed that the inhibitory effect of chloro-substituted phenols on bioluminescence of the ahs-mutants depended on their hydrophobicity. The effect of chlorinated phenols and detergents on bioluminescence was increased in the ahs-mutants. The potential use of these mutants in bioluminescent toxicity tests was discussed.
Wagner
A
A
Lehrstuhl f. Biologie d. Mikroorganismen, Ruhr-Universität, Bochum, FRG.
Winkler
U
U
Lümmen
P
P
D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002463:Cell Membrane Permeability; D003902:Detergents / Q000633:toxicity; D004353:Drug Evaluation, Preclinical; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity; D008163:Luminescent Measurements; D009154:Mutation; D013329:Structure-Activity Relationship
D003902:Detergents; D006843:Hydrocarbons, Chlorinated