Effects of (+/-)-DOI on medial prefrontal cortical cells: a microiontophoretic study.
The relatively selective 5-HT2 receptor agonist, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane [+/-)-DOI) ejected by microiontophoresis at low currents potentiates glutamate (GLU)-induced excitation and at higher currents (greater than 20 nA) invariably inhibits both spontaneous and GLU-induced activity of cells in the medial prefrontal cortex (mPFc). The inhibitory action of DOI is blocked by the 5-HT2 antagonists, ritanserin, metergoline and spiperone, but not other receptor antagonists. Microiontophoresis of 1 M Mg2+ for 5-20 min did not alter DOI's inhibitory effect suggesting that DOI's action is a direct one. These results show that DOI predominantly inhibits mPFc neuronal activity and this inhibitory action is mediated by 5-HT2 receptors.
Ashby
C R
CR
Department of Psychiatry and Behavioral Sciences, State University of New York, Stony Brook 11794-8790.
Edwards
E
E
Harkins
K
K
Wang
R Y
RY
D000662:Amphetamines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004594:Electrophysiology; D005625:Frontal Lobe / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007478:Iontophoresis; D015636:Magnesium Chloride / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D012702:Serotonin Antagonists / Q000494:pharmacology; D013237:Stereoisomerism
D000662:Amphetamines; D012702:Serotonin Antagonists; D015636:Magnesium Chloride; C015952:4-iodo-2,5-dimethoxyphenylisopropylamine