Design of coupled reactions for simplification of bioluminescence analysis.
Luminescence analysis may be applied to many substances by arranging a prior reaction producing a species entering the light-emitting reaction. Under favourable conditions the two consecutive reactions are carried out simultaneously as a one-step procedure. In a bioluminescence assay, luciferase stability is frequently a problem, making it desirable to develop analytical schemes where the analytical response becomes largely independent of any impaired luciferase activity. The value of maximal emission or an approached steady-state level is a convenient and usually well-defined analytical parameter. When recording this level it is important to design the participating reactions in a way that compensates for changes in luciferase activity.
Brolin
S E
SE
Department of Medical Cell Biology, University of Uppsala, Sweden.
Naeser
P
P
Wettermark
G
G
D055598:Chemical Phenomena; D002621:Chemistry; D004798:Enzymes / Q000032:analysis; D007700:Kinetics; D008156:Luciferases; D008163:Luminescent Measurements; D009711:Nucleotides / Q000032:analysis
D004798:Enzymes; D009711:Nucleotides; D008156:Luciferases