[Study of the carbohydrate components of the surface of S 1300 neuroblastoma cells with the use of cytochemical and lectin agglutination methods].
The carbohydrate determinants of the cell surface of neuroblastoma C 1300 clone N 18 have been investigated by light microscopy with a panel of 11 lectins as cytochemical reagents. The expression of N- and O-glycanes with a predominance of 3-4 antigenic N-glycosyl chains was revealed. The characteristic feature of neuroblastoma cells is an abundance of an antigen A surface determinant. A strong correlation is shown between the intensity of lectin binding and index of cell agglutination. After the binding of lectins with living cells the lectin-receptor complexes are clustered, endocytosed and for 4 hours are concentrated as microvesicles in the cytoplasm compact area. Heterogeneity of the neuroblastoma cell population on the binding of peanut and Lens culinaris lectins is revealed.
Tsegel'skiĭ
A A
AA
Lutsik
M D
MD
Lashkaĭ
A F
AF
D000372:Agglutination Tests; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D037102:Lectins
Issledovanie uglevodnykh komponentov poverkhnosti kletok neĭroblastomS 300 s pomoshch'iu metodov tsitokhimii i agliutinatsii lektinami.