title: States of the art of immunoelectron microscopy in Japan.
abstract: In Japan, there are several laboratories which routinely utilize immunoelectron microscopy (IEM) for their investigation and participate in the development of methods for IEM. As a result, some major advances are in the making. IEM methods used in Japan may be divided roughly into four categories: 1) antibodies are reacted with tissues before the tissues are embedded in plastics (pre-embedding method); 2) antibodies are reacted with tissues after the tissues are embedded in plastics (post-embedding method); 3) tissues reacted with antibodies are deep-etched and the site of antigens are recognized in the replicas; and 4) that uses scanning electron microscopes. Samples from each category are introduced utilizing photographs provided by investigators, representing the states of the art of IEM in Japan.
authors: P K
authors: PK
authors: Nakane
mesh_terms: D000818:Animals; D007118:Immunoassay / Q000379:methods*; D008854:Microscopy, Electron; D051381:Rats