title: [Increase in the in vitro activity of mollicute transcriptase using substances of an organic nature].
abstract: It is established that the use of DNA-dependent RNA-polymerases of mollicutes in the molecular-biological and gene-engineering works aimed to obtain a sufficient amount of the transcription products may promote a 1.5-2-fold increase of the yield by introducing such substances as glycerol, dimethylsulphoxide and spermidine (optimal concentrations: 10-12 vol. % and 12 mM, respectively) into the composition of the reacting mixture.
authors: I G
authors: IG
authors: Skripal'
authors: V V
authors: VV
authors: Babichev
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology*; D009174:Mycoplasma / Q000187:drug effects / Q000201:enzymology*; D011073:Polyamines / Q000494:pharmacology*
chemical_list: D011073:Polyamines; D012321:DNA-Directed RNA Polymerases; D005990:Glycerol; D004121:Dimethyl Sulfoxide
vernacular_title: Povyshenie in vitro aktivnosti transkriptaz mollikutov pri pomoshchi veshchestv organicheskoĭ prirody.