title: The effect of macrophage-conditioned medium on metabolism of normal very low density lipoprotein by fibroblasts.
abstract: Triglyceride was accumulated saturably in cultivated human fibroblasts exposed to increasing concentration of normal very low density lipoprotein (N-VLDL). Characterization of binding and degradation of 125I-N-VLDL by the cells indicated a direct uptake of intact N-VLDL particles via the cell surface receptor. Competition of unlabelled N-VLDL and LDL with 125I-N-VLDL in fibroblasts suggested that LDL receptor may be involved in this process. An unsaturable triglyceride accumulation in fibroblast induced by macrophage-conditioned medium containing N-VLDL was also observed. Intracellular triglyceride content, in this case, is linearly correlated with the concentration of N-VLDL in the medium and results mainly from re-esterification of fatty acid produced by hydrolysis of VLDL-triglyceride by lipoprotein lipase of macrophage.
authors: S P
authors: SP
authors: Wang
authors: Z C
authors: ZC
authors: Feng
authors: W S
authors: WS
authors: Wu
authors: C B
authors: CB
authors: Wang
authors: W G
authors: WG
authors: Jiang
authors: K
authors: K
authors: Xu
mesh_terms: D001897:Boronic Acids; D003470:Culture Media; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001897:Boronic Acids; D003470:Culture Media; D008079:Lipoproteins, VLDL; D014280:Triglycerides; C010686:benzeneboronic acid