Early responses by osteoclasts in vivo and dentinoclasts in vitro to corticosteroids.
The results of previous investigations examining the association between corticosteroids and bone resorption in vitro have been conflicting. Recent studies have shown that application of a triamcinolone-containing medicament to the dental pulp cavity inhibits dentinoclast-mediated tooth resorption in vivo and in vitro. The aims of this study were to quantitate early ultrastructural changes in osteoclasts in vivo in response to a single steroid dose, and to examine the attachment of dentinoclasts grown in steroid-supplemented cultures in vitro. Resorbing bone around erupting rat molars was examined using TEM and morphometrically quantitated for changes in ruffled border and clear zone areas 1 and 4 h after injections of NaCl, calcitonin, hydrocortisone and prednisolone. Dentinoclasts were harvested from resorption areas in mature rat molars, grown in cultures with calcitonin, hydrocortisone or prednisolone, and fixed and viewed with SEM after 30 min and 2 h. Results of TEM studies showed a significant reduction in areas of resorbing structures in osteoclasts treated with calcitonin at 1 and 4 h, and prednisolone at 4 h. At 1 and 4 h, hydrocortisone showed an increase in resorbing structure areas. SEM results indicated all experimental substances reduced dentinoclast spreading and attachment. It was concluded that the direct effect of steroids on clastic cells may be one of inhibition (the degree of which depends on the nature and dose of the steroid) whereas, in vivo, systemic administration may cause more secondary effects (such as PTH stimulation) to compete with this inhibition. The efficacy of steroid-containing medicaments in inhibiting dentinoclastic resorption may be due to their relatively localized area of application.
Pierce
A M
AM
Department of Pathology, University of Adelaide, Australia.
Lindskog
S
S
D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000818:Animals; D001862:Bone Resorption / Q000139:chemically induced; D002116:Calcitonin / Q000494:pharmacology; D002452:Cell Count; D002478:Cells, Cultured; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D003804:Dentin / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D006854:Hydrocortisone / Q000494:pharmacology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010010:Osteoclasts / Q000187:drug effects* / Q000648:ultrastructure; D011239:Prednisolone / Q000494:pharmacology; D051381:Rats
D000305:Adrenal Cortex Hormones; D002116:Calcitonin; D011239:Prednisolone; D006854:Hydrocortisone