title: Expression of normal and mutant avian integrin subunits in rodent cells.
abstract: We describe the expression of the beta 1 subunit of avian integrin in rodent cells with the purpose of examining the structure-function relationships of various domains within this subunit. The exogenous subunit is efficiently and stably expressed in 3T3 cells, and it forms hybrid heterodimers with endogenous murine alpha subunits, including alpha 3 and alpha 5. These heterodimers are exported to the cell surface and localize in focal contacts where both extracellular matrix and cytoskeleton associate with the plasma membrane. Hybrid heterodimers consisting of exogenous beta 1 and endogenous alpha subunits bind effectively and specifically to columns of cell-binding fragments of fibronectin. The exogenous avian beta 1 subunit appears to function as well as its endogenous murine equivalent, consistent with the high degree of conservation noted previously for integrins. In contrast, expression of a mutant form of avian integrin beta 1 subunit lacking the cytoplasmic domain produces hybrid heterodimers which, while efficiently exported to the cell surface and still capable of binding fibronectin, do not localize efficiently in focal contacts. This further implicates the cytoplasmic domain of the beta 1 subunit in interactions required for cytoskeletal organization.
authors: Wistar Institute, Philadelphia, Pennsylvania 19104.
authors: J
authors: J
authors: Solowska
authors: J L
authors: JL
authors: Guan
authors: E E
authors: EE
authors: Marcantonio
authors: J E
authors: JE
authors: Trevithick
authors: C A
authors: CA
authors: Buck
authors: R O
authors: RO
authors: Hynes
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005786:Gene Expression Regulation; D016023:Integrins; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D005353:Fibronectins; D016023:Integrins; D008562:Membrane Glycoproteins; D010446:Peptide Fragments