Interleukin-1 beta stimulates phospholipase A2 mRNA synthesis in rabbit articular chondrocytes.
A cDNA that codes for human rheumatoid synovial fluid phospholipase A2 (PLA2) hybridized with a RNA of the same size (900 base pairs) isolated from rabbit articular chondrocytes (RAC). Treatment of RAC with 100 ng/ml recombinant human interleukin-1 beta (IL-1) for 24 hours caused a 13-fold increase in mRNA for this PLA2. Timecourse studies demonstrated that maximal induction of PLA2 mRNA occurred by 16 hours post addition of IL-1 (100 ng/ml). Augmentation of RAC PLA2 mRNA levels was dose-dependent; half-maximal induction was estimated to require 0.15 ng/ml IL-1. Actinomycin D inhibited IL-1 effects on PLA2 mRNA levels. Coordinate effects of IL-1 on RAC PLA2 activity were observed with respect to time and dose dependence as well as actinomycin D sensitivity.
Lyons-Giordano
B
B
Medical Products Department E. I. Du Pont de Nemours and Company, Inc., Wilmington, DE 19880-0400.
Davis
G L
GL
Galbraith
W
W
Pratta
M A
MA
Arner
E C
EC
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000201:enzymology; D015152:Blotting, Northern; D002358:Cartilage, Articular / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D003609:Dactinomycin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D009693:Nucleic Acid Hybridization; D010740:Phospholipases / Q000235:genetics*; D010741:Phospholipases A / Q000235:genetics* / Q000378:metabolism; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D011817:Rabbits; D013582:Synovial Fluid / Q000201:enzymology; D014158:Transcription, Genetic
D015342:DNA Probes; D007375:Interleukin-1; D012333:RNA, Messenger; D003609:Dactinomycin; D004247:DNA; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2