The sequence of the mouse 14 kDa beta-galactoside-binding lectin and evidence for its synthesis on free cytoplasmic ribosomes.
The partial amino acid sequence of the mouse 14 kDa beta-galactoside-binding lectin has been deduced from cDNA clones corresponding to 86% of the coding sequence and extending to the polyadenylation signal. The deduced amino acid sequence for the murine lectin shows 94% identity with the rat, 89% with human, 86% with bovine and 46% with the chicken 14 kDa lectins. A cDNA probe has been used to analyse genomic DNA and identify a single mRNA of approx. 570 bp in 3T3 fibroblasts, murine erythroleukaemia cells and the murine basement-membrane-secreting Engelbreth-Holm-Swarm tumour. Analysis of free and bound polyribosomes has shown that the lectin message is translated on free cytoplasmic ribosomes.
Wilson
T J
TJ
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
Firth
M N
MN
Powell
J T
JT
Harrison
F L
FL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis*; D005697:Galactosides; D037161:Galectins; D006027:Glycosides; D006388:Hemagglutinins; D051379:Mice; D008969:Molecular Sequence Data; D012270:Ribosomes / Q000378:metabolism*
D002352:Carrier Proteins; D005697:Galactosides; D037161:Galectins; D006027:Glycosides; D006388:Hemagglutinins