[Studies on the control mechanism of hCG beta gene expression--focusing on identification of promoter and other transcriptional regulatory elements].
hCG beta gene is composed of 6 genes or pseudogenes. To find which of these 6 genes are active and which are inactive, a cosmid clone containing the entire hCG beta gene cluster, which we had isolated previously, was separated into subclones containing each gene. Then they were transfected into mouse Y1 cells which express this gene efficiently. The result showed that genes 5, 3 and 8 are active and the rest are inactive. To identify the promoter region of gene 5, which is the most active gene, we created deletion mutants lacking various lengths of gene 5 upstream sequence. We made them either by using restriction endonuclease or Exonuclease III. We transfected them into Y1 cells and studied which part of the upstream sequence is required for hCG beta expression. The results of this experiment show that the promoter region for hCG beta is located within 78bp of the cap site and there are additional regulatory elements upstream. The information obtained here provides a foundation for analytical studies on nuclear factors binding to this region regulating hCG beta expression.
Otani
T
T
Department of Obstetrics and Gynecology, Kobe University School of Medicine.
Boime
I
I
Mochizuki
M
M
D006063:Chorionic Gonadotropin / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D005786:Gene Expression Regulation; D006801:Humans; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D006063:Chorionic Gonadotropin