Structure of a novel glucosamine-containing phosphoglycolipid from Deinococcus radiodurans.
The structure of a major novel lipid from Deinococcus radiodurans has been determined to be 2'-O-(1,2-diacyl-sn-glycero-3-phospho)-3'-O-(alpha-N-acetylglucosaminyl) -N- glyceroyl alkylamine. The lipid was shown to contain a phosphatidic acid backbone by digestion with phospholipase A2 and by hydrolysis with hydrofluoric acid. Using a combination of chemical and NMR spectroscopic techniques, the structure of this lipid was elucidated and compared with that of a similar phosphoglycolipid reported earlier (Anderson, R., and Hansen, K. (1985) J. Biol. Chem. 260, 12219-12223) in which galactose was found in place of N-acetylglucosamine. The fatty acid compositions of the two lipids were similar.
Huang
Y
Y
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.
Anderson
R
R
D000107:Acetylation; D002241:Carbohydrates / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005944:Glucosamine / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis* / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000032:analysis*; D006858:Hydrofluoric Acid; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010712:Phosphatidic Acids / Q000032:analysis; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2
D002241:Carbohydrates; D005227:Fatty Acids; D006017:Glycolipids; D010712:Phosphatidic Acids; D010741:Phospholipases A; D054467:Phospholipases A2; D005944:Glucosamine; D006858:Hydrofluoric Acid