Induction and synthesis of nitrate reductase in Chlorella vulgaris.
Assimilatory nitrate reductase is an inducible, eukaryotic enzyme that responds to a variety of environmental cues. When higher plants and green algae are grown with ammonia as a nitrogen source, low levels of nitrate reductase activity are present. Transfer to nitrate-containing medium is accompanied by substantial increase of nitrate reductase activity. Here it is shown immunologically that, in the green algae Chlorella vulgaris, nitrate reductase protein is over-produced as activity appears during induction. Immunoreactive protein is also found in cells grown on ammonia. Low levels of translatable mRNA for nitrate reductase are present in ammonia-grown cells. These data suggest that: (i) nitrate reductase appearance is controlled primarily on a transcriptional level, but that transcription is not completely halted under repressing conditions; (ii) there is an overproduction of nitrate reductase protein early during the induction period as previously suggested; and (iii) nascent protein, from in vitro translation, is of approximately the same molecular size as the nitrate reductase subunit and therefore little posttranslational modification is necessary to generate the functional enzyme. Insertion of cofactors and assembly are probably the only post-translational events.
Sherman
T D
TD
Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843.
Funkhouser
E A
EA
D002708:Chlorella / Q000201:enzymology*; D004790:Enzyme Induction; D015151:Immunoblotting; D008970:Molecular Weight; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000096:biosynthesis* / Q000235:genetics; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
D012333:RNA, Messenger; D009565:Nitrate Reductases; D050901:Nitrate Reductase