Effects of tenotomy on muscle histology and energy metabolism in normal and dystrophic mice.
Creatine, phosphocreatine, ATP and phosphate concentrations were measured in extracts of tibialis anterior and gastrocnemius muscles from 42 normal and 39 dystrophic mice from 1 to 16 weeks of age. No differences were observed at 1 week, prior to the onset of histological abnormalities in dystrophic animals. Creatine and phosphocreatine concentrations were significantly reduced in older dystrophic mice, and phosphate levels were higher, while ATP levels generally did not differ. Tenotomy of gastrocnemius at 1 week prevented the development of dystrophy in this muscle but this was not associated with an increase in phosphogen concentrations. Serum creatine kinase levels were significantly higher in dystrophic mice than normal mice but only during the first two weeks of life; levels in older mice were not significantly different. This study shows that the reported deficits in phosphogen concentrations in dystrophic muscles are likely to reflect the results, rather than the cause, of the dystrophic process.
Lane
R J
RJ
Muscular Dystrophy Group Research Laboratories, Newcastle General Hospital, U.K.
Watmough
N J
NJ
Jaros
E
E
D000367:Age Factors; D000818:Animals; D003402:Creatine Kinase / Q000097:blood*; D004734:Energy Metabolism; D051379:Mice; D009132:Muscles / Q000294:innervation* / Q000378:metabolism / Q000503:physiopathology; D009137:Muscular Dystrophy, Animal / Q000378:metabolism* / Q000503:physiopathology; D009929:Organ Size
D003402:Creatine Kinase