title: A fluorescence sandwich ELISA for detecting soluble and cell-associated human interleukin-2.
abstract: A fluorescence sandwich ELISA was developed for the quantitation of human interleukin-2 (IL-2) using a combination of two anti-human IL-2 antibodies, L61 and biotinylated anti-IL-2 rabbit IgG. The minimum level of IL-2 detectable by this ELISA was 0.3 pg/ml, indicating that this method is about 100 times more sensitive than the usual colorimetric human IL-2 ELISA. The ELISA specifically detected human IL-2 in cell culture supernatants and serum, and also in extracts from mitogen-stimulated peripheral blood mononuclear cells. All of the IL-2 species detected in the supernatants, sera and cell lysates were shown to have a molecular weight of 15,000, as estimated by size exclusion high performance liquid chromatography.
authors: AIDS Research Center, National Institute of Health, Tokyo, Japan.
authors: K
authors: K
authors: Kitamura
authors: K
authors: K
authors: Matsuda
authors: M
authors: M
authors: Ide
authors: T
authors: T
authors: Tokunaga
authors: M
authors: M
authors: Honda
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D013045:Species Specificity
chemical_list: D007376:Interleukin-2