Zinc increases phorbol ester receptors in intact B-cells, neutrophil polymorphs and platelets.
In the presence of pyrithione, which was used as a Zn2+ ionophore, Zn2+ (10-100 microM) increased phorbol ester binding by intact B-CLL cells in a dose-dependent fashion. Zn pyrithione increased 2-fold the number of phorbol ester receptors in B-cells (0.74 to 1.4 pmol/10(6) cells), neutrophil polymorphs (0.2 to 0.51 pmol/10(6) cells) and platelets (91 to 209 pmol/10(10) cells). Fractionation of cells after treatment with Zn pyrithione showed that increased binding of PDBu occurred in the particulate fraction of cells and this was accompanied by loss of phorbol ester receptors from the cytosol. These data are compatible with a role for Zn in the subcellular distribution and activation of protein kinase C.
Forbes
I J
IJ
Department of Medicine, University of Adelaide, Queen Elizabeth Hospital, Woodville, Australia.
Zalewski
P D
PD
Hurst
N P
NP
Giannakis
C
C
Whitehouse
M W
MW
D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D006801:Humans; D007700:Kinetics; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010618:Phenanthrolines / Q000494:pharmacology; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D011955:Receptors, Drug / Q000187:drug effects / Q000378:metabolism*; D013871:Thiones; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000494:pharmacology*
D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D010618:Phenanthrolines; D011725:Pyridines; D011955:Receptors, Drug; D013871:Thiones; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D015240:Phorbol 12,13-Dibutyrate; C008704:pyrithione; D011493:Protein Kinase C; D015032:Zinc