A rapid, qualitative ELISA test for the specific detection of morphine in serum or urine.
We describe a competitive inhibition ELISA technique, with a visual end-point, to detect free morphine in blood or urine. It has a sensitivity of 2 X 10(-7) mol/l using 5 microliter samples. No significant cross-reactivity was observed with other opiate derivatives. The assay has applications as a specific screen for morphine in drug abusers, or to study the metabolism of the drug in the body (as the metabolite, morphine-3-glucuronide, does not cross-react significantly with morphine in the assay).
Laurie
D
D
School of Pharmaceutical and Chemical Sciences, Sunderland Polytechnic, Sunderland, UK.
Manson
A J
AJ
Rowell
F J
FJ
Seviour
J
J
D000328:Adult; D003061:Codeine / Q000652:urine; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005966:Glucuronidase / Q000494:pharmacology; D006556:Heroin Dependence / Q000652:urine; D006801:Humans; D008297:Male; D008722:Methods; D008875:Middle Aged; D009020:Morphine / Q000032:analysis* / Q000097:blood / Q000652:urine; D009021:Morphine Dependence / Q000652:urine; D009294:Narcotics / Q000652:urine; D011863:Radioimmunoassay; D012015:Reference Standards
D009294:Narcotics; D009020:Morphine; D005966:Glucuronidase; D003061:Codeine