title: Evolution of compound centromeres. A new phenomenon.
abstract: A new type of centromere aberration in a transformed cell line of rat cerebral endothelial origin is described. These cells exhibit normal monocentric, dicentric, and multicentric chromosomes. The centromeres in dicentrics and multicentrics express variable locations along the chromosome. The centromeres in some of the multicentrics are located next to each other, with small intervening noncentromeric chromatin. In others, the centromeres appear to be in the immediate vicinity of each other with no evidence of intervening chromatin. This organization of the centromeres results in what appears to be a compound centromere composed of some multiples of single centromeres. All centromeres deposit kinetochore proteins that respond to kinetochore antibody. This evidence and that obtained from electron microscopy permits the conclusion that various centromeres/kinetochores in the compound structure are functional. The study presented here points to the existence of compound large centromeres--a novel phenomenon in cytogenetics--that may be prevalent in cancer cells. In the present cell line these regions appear as long, neck-like structures in some chromosomes and may be similar to some in vivo situations such as the X in Indian muntjac.
authors: Institute fur Zell- und Tumor Biologie, Deutsches Krebsforschungszentrum, Heidelberg, BRD.
authors: N
authors: N
authors: Paweletz
authors: B K
authors: BK
authors: Vig
authors: E M
authors: EM
authors: Finze
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002461:Cell Line, Transformed; D002503:Centromere / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure*; D004727:Endothelium / Q000648:ultrastructure; D007621:Karyotyping; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011921:Rats, Inbred WKY