Production of interleukin-1-alpha and -beta by human peripheral polymorphonuclear neutrophils.
The mechanism of the production of interleukin-1 (IL-1) by human peripheral polymorphonuclear neutrophils (PMN) was investigated. Supernatants of PMN stimulated with 30 micrograms/ml lipopolysaccharide (LPS) were used as extracellular IL-1 and supernatants of their lysate as intracellular IL-1 source. IL-1 activity was measured by the C3H/HeJ thymocyte co-mitogenic assay. The supernatants from PMN stimulated with LPS for 72 h showed IL-1 activity which had an apparent molecular weight of 15-20 kilodaltons and pI of 5.0 and more than 8.5. It was neutralized with anti-IL-1 antibodies and it lacked IL-2 activity. Our time course study of the IL-1 assay with neutralization by anti-IL-1-alpha and -beta antibodies indicated that the extracellular IL-1-beta activity appeared predominantly in the early incubation periods, whereas alpha activity appeared predominantly in the late periods. Intracellular IL-1-alpha but not beta activity was detected mainly at the intermediate incubation periods. These data indicate that PMN stimulated with LPS produce both IL-1-alpha and -beta, and release IL-1-beta first and IL-1-alpha later.
Goh
K
K
Department of Dermatology, Teikyo University School of Medicine, Tokyo, Japan.
Furusawa
S
S
Kawa
Y
Y
Negishi-Okitsu
S
S
Mizoguchi
M
M
D000818:Animals; D002470:Cell Survival; D002474:Cell-Free System; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000032:analysis; D005110:Extracellular Space / Q000032:analysis; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis*; D007525:Isoelectric Focusing; D007700:Kinetics; D008070:Lipopolysaccharides; D051379:Mice; D008809:Mice, Inbred C3H; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology
D007375:Interleukin-1; D008070:Lipopolysaccharides