The induction of DNA-strand breaks and unscheduled DNA synthesis in F-344 rat hepatocytes following in vivo administration of caprolactam or benzoin.
Benzoin (ZOIN) and caprolactam (CAP) were administered by gavage to Fischer 344 rats at a dose of 750 mg/kg and the hepatocytes isolated 12, 24 or 48 h after treatment. The isolated hepatocytes were subsequently examined for the induction of DNA-strand breaks (SB) and unscheduled DNA synthesis (UDS). Neither ZOIN nor CAP induced SB or UDS in hepatocytes, however ZOIN did induce an increase in the fraction of cells in S-phase 24 h after treatment. These results correlate well with the observed lack of carcinogenicity of these compounds.
Bermudez
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Department of Genetic Toxicology, Chemical Industry Institute of Toxicology, Research Triangle Park, NC.
Smith-Oliver
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T
Delehanty
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LL
D000818:Animals; D001381:Azepines / Q000633:toxicity*; D001573:Benzoin / Q000633:toxicity*; D002209:Caprolactam / Q000633:toxicity*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D009153:Mutagens; D051381:Rats; D011916:Rats, Inbred F344
D001381:Azepines; D009153:Mutagens; D002209:Caprolactam; D001573:Benzoin