title: Effects of serine protease inhibitors on oxyradical burst from phagocytizing neutrophils--analysis by chemiluminescence counting and its microscopic imaging.
abstract: Reaction difference of oxyradical generation and luminol-dependent photoemission of zymosan- and phorbol ester-treated neutrophils were investigated using a conventional photomultiplier and ultrasensitive photonic imaging technique. Zymosan-treated cells released a concentrated photonic burst corresponding to the cellular distribution. In contrast, phorbol ester-treated cells produced a negligible level of photoemission, and the additional application of Ca2+ ionophore enhanced the photonic burst, which was gradually spread out into extracellular space. Serine protease inhibitors did not attenuate PMA-induced chemiluminescence but did attenuate zymosan-induced chemiluminescence. This suggests the involvement of serine protease in the respiratory burst of phagocytizing neutrophils.
authors: Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
authors: M
authors: M
authors: Suematsu
authors: S
authors: S
authors: Houzawa
authors: S
authors: S
authors: Miura
authors: H
authors: H
authors: Nagata
authors: T
authors: T
authors: Kitahora
authors: T
authors: T
authors: Morishita
authors: C
authors: C
authors: Oshio
authors: M
authors: M
authors: Tsuchiya
mesh_terms: D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010587:Phagocytosis; D015842:Serine Proteinase Inhibitors; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D005609:Free Radicals; D015842:Serine Proteinase Inhibitors; D008165:Luminol; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen