Enhanced production of interleukin-6 in mice with type II collagen-induced arthritis.
We established an interleukin-6 (IL-6)-dependent cell line from murine plasmacytoma MOPC-104E cells. This cell line (designated PIL-6) was found to respond to murine and to human IL-6, but not to any other cytokines. We used this cell line to investigate the involvement of IL-6 production in type II collagen-induced arthritis in DBA/1 mice. Only marginal IL-6 activity was detected in sera from DBA/1 mice inoculated with Freund's complete adjuvant (FCA) alone, with an unrelated protein (bovine serum albumin) plus FCA, or with type II collagen plus Freund's incomplete adjuvant. However, enhanced IL-6 activity was observed in DBA/1 mice that had been injected with type II collagen plus FCA to induce arthritis. The elevated level of serum IL-6 activity was associated with high levels of IL-6 produced when lymph node cells from arthritic mice were stimulated in vitro with type II collagen. We also found that the L3T4+ T cell subset is responsible for the enhanced production of IL-6 in arthritic mice. The results are discussed in the context of potential roles of IL-6 in the induction and/or expression of chronic, progressive arthritis.
Takai
Y
Y
Department of Oncogenesis, Osaka University Medical School, Japan.
Seki
N
N
Senoh
H
H
Yokota
T
T
Lee
F
F
Hamaoka
T
T
Fujiwara
H
H
D000818:Animals; D001168:Arthritis / Q000209:etiology / Q000378:metabolism*; D001169:Arthritis, Experimental / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000276:immunology / Q000494:pharmacology; D007114:Immunization; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis* / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D012710:Serum Albumin, Bovine / Q000276:immunology
D015850:Interleukin-6; D007378:Interleukins; D012710:Serum Albumin, Bovine; D003094:Collagen