Use of deuterated internal standards for quantitation of T-2 and HT-2 toxins in human blood by tandem mass spectrometry.
Deuterated acetyl derivatives (3-trideutero-acetyl-T-2 and 15-trideutero-HT-2) were prepared for use as internal standards for the quantitation of T-2 and HT-2 in blood by tandem mass spectrometry. The method used was multiple reaction monitoring (MRM), which essentially involves the selection of a parent ion for analysis followed by monitoring of the daughter ions generated by collision activated decomposition. The parent ions chosen for the trifluoroacetate derivative of T-2 and HT-2 were m/z+ 478 and 532, respectively. Both parents yield the same daughter ions, i.e., 180, 138, and 121. HT-2 and T-2 were added to blood extracts in amounts ranging from 1 to 20 ppb. The limit of detection is about 0.5 ppb with an effective detection limit of 1.0 ppb in a range of 1-20 ppb. The recovery is about 90%. This method can be used by veterinarians for purposes of diagnostics. It can be used for urine as well as blood.
Pawlosky
R J
RJ
University of Minnesota, Department of Plant Pathology, St. Paul 55108.
Mirocha
C J
CJ
Wen
Y
Y
Abbas
H K
HK
D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D012717:Sesquiterpenes / Q000097:blood*; D013605:T-2 Toxin / Q000097:blood* / Q000652:urine / Q000031:analogs & derivatives
D007202:Indicators and Reagents; D012717:Sesquiterpenes; D013605:T-2 Toxin; C012351:HT-2 toxin