Chemical synthesis of the 24 RNA fragments corresponding to hop stunt viroid.
A general and practical synthetic method of oligoribonucleotides (10-20 mers) by using the cyanoethyl phosphoramidite approach was described. In this experiment 9-phenylxanthen-9-yl (Pix) and 9-(4-methoxy)phenylxanthen-9-yl (Mox) groups were employed for the 5'-hydroxyls and tetrahydropyranyl (Thp) group was used for the 2'-hydroxyl protecting groups. In addition, suitable acyl groups were introduced for the protection of the lactam functions of guanine and uracil moieties.
Tanimura
H
H
Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan.
Maeda
M
M
Fukazawa
T
T
Sekine
M
M
Hata
T
T
D001483:Base Sequence; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010942:Plant Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000138:chemical synthesis*; D014772:Viroids / Q000235:genetics*
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012367:RNA, Viral