Identification and characterization of a serotype b-specific segment of the Haemophilus influenzae genome.
Within the region of the Haemophilus influenzae serotype b genome required for capsule expression, a 4.6-kilobase segment was cloned, used as a hybridization probe, and found to be specific for serotype b strains. Deletion mutagenesis within this segment defined a locus necessary for serotype b capsule production and another involved in maintenance of wild-type colony appearance.
Ely
S
S
Department of Paediatrics, John Radcliffe Hospital, Oxford, Headington, United Kingdom.
Tippett
J
J
Moxon
E R
ER
D002872:Chromosome Deletion; D004269:DNA, Bacterial / Q000302:isolation & purification*; D015246:Deoxyribonuclease EcoRI; D005798:Genes, Bacterial; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics*; D009154:Mutation; D012091:Repetitive Sequences, Nucleic Acid; D012703:Serotyping
D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI