title: Anaphylactoid reactions mediated by autoantibodies to cholesterol in miniature pigs.
abstract: Antoantibodies to cholesterol were detected and purified from normal (nonimmunized) pig serum. The antibodies were assayed by ELISA with crystalline cholesterol as an Ag and by C-dependent damage to cholesterol-laden liposomes. Intravenous injection of liposomes containing cholesterol into anesthetized animals caused decreased hemolytic complement titers, and induced a reaction consisting of transient neutropenia, thrombocytopenia, respiratory distress, cyanosis, pulmonary and systemic hypertension, and decreased cardiac output. Plasma levels of thromboxane B2 and 6-keto-prostaglandin F1 alpha increased 1300 and 200%, respectively, and leukocyte and platelet counts decreased by 36 and 38%, respectively. Injection of cholesterol-free liposomes did not induce the reaction. These results show that naturally occurring autoantibodies to cholesterol can initiate C activation and can be associated with anaphylactoid reaction to exogenously administered cholesterol in pigs.
authors: Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
authors: N M
authors: NM
authors: Wassef
authors: S H
authors: SH
authors: Johnson
authors: G M
authors: GM
authors: Graeber
authors: G M
authors: GM
authors: Swartz
authors: C L
authors: CL
authors: Schultz
authors: J R
authors: JR
authors: Hailey
authors: A J
authors: AJ
authors: Johnson
authors: D G
authors: DG
authors: Taylor
authors: R L
authors: RL
authors: Ridgway
authors: C R
authors: CR
authors: Alving
mesh_terms: D000707:Anaphylaxis / Q000276:immunology* / Q000503:physiopathology; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D002784:Cholesterol / Q000276:immunology*; D003167:Complement Activation; D015777:Eicosanoids / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007213:Indomethacin / Q000627:therapeutic use; D007958:Leukocyte Count; D010976:Platelet Count; D013552:Swine
chemical_list: D001323:Autoantibodies; D015777:Eicosanoids; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D002784:Cholesterol; D007213:Indomethacin