Artefactual low lymphocyte activity caused by platelet contamination in the mononuclear cell preparations.
Platelet (PLT) contamination in preparations of mononuclear cells (MNC) produces a significant decrease (P less than 0.02) in lymphoproliferative activity and interleukin 2 (IL2) production. This effect was observed in MNC preparations from either healthy blood donors or polyglobulic patients. In a dose-response curve carried out with increasing PLT number per MNC, progressive inhibition was found, reaching to 62.6% and 49.5%, respectively, to phytohemagglutinin and IL2 lymphoproliferative response and 87.8% in IL2 production, at 80 PLTs/MNC.
Rueda
F
F
Unitat de Recerca Biomedica, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Marti
F
F
Piñol
G
G
Remacha
A
A
D001792:Blood Platelets / Q000502:physiology*; D002455:Cell Division; D006403:Hematologic Tests; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D009000:Monocytes / Q000502:physiology*; D010835:Phytohemagglutinins; D011087:Polycythemia Vera / Q000097:blood*
D007376:Interleukin-2; D010835:Phytohemagglutinins