Binding of epidermal growth factor and insulin-like growth factor I in human myometrium and leiomyomata.
Samples of uterine myometrium and leiomyoma from 11 women were analyzed for the presence of epidermal growth factor receptors and insulin-like growth factor I receptors. In addition, the content of soluble insulin-like growth factor binding protein (IGF-BP/PP12) was measured in the tissue cytosols. Cell membrane preparations of myoma tissue bound significantly more insulin-like growth factor I than did those of adjacent normal myometrium, whereas myoma tissue bound less epidermal growth factor than did the normal myometrium. The differences in both insulin-like growth factor I and epidermal growth factor binding were due to changes in receptor concentration rather than to alterations in receptor affinity. Neither myoma nor myometrial tissue contained detectable levels of insulin-like growth factor binding protein. The changes in epidermal growth factor and insulin-like growth factor I binding to the myometrium may play a role in the pathogenesis of uterine leiomyomata.
Tommola
P
P
Minerva Institute for Medical Research, Kauniainen, Finland.
Pekonen
F
F
Rutanen
E M
EM
D000328:Adult; D001665:Binding Sites; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007889:Leiomyoma / Q000378:metabolism*; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism*; D013002:Somatomedins / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000378:metabolism*
D007457:Iodine Radioisotopes; D013002:Somatomedins; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I