The possible absence of cytochrome P-450 linked xenobiotic metabolism in helminths.
The distribution of cytochrome P-450, b5 and associated oxidations, aniline hydroxylase, 7-ethoxycoumarin O-deethylase and 4-nitroanisole O-demethylase were studied in the parasitic nematode Heligmosomoides polygyrus, its mammalian host, Mus musculus and the free-living nematode Panagrellus redivivus. Cytochrome P-450, its associated oxidations and cytochrome b5 could not be detected in whole homogenates or subcellular fractions (mitochondrial, microsomal and soluble fractions) of either nematode under a variety of assay conditions which included attempted induction with sodium phenobarbital. P. redivivus was able to reduce 1,2-dimethyl-4-(4-carboxyphenylazo)-5-hydroxybenzene and azobenzene, which is predominantly microsomal. The implications of these results in terms of chemotherapy are discussed.
Precious
W Y
WY
Department of Biological Sciences, University College of Wales, Aberystwyth.
Barrett
J
J
D015660:7-Alkoxycoumarin O-Dealkylase; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003573:Cytochrome b Group / Q000378:metabolism; D015786:Cytochromes b5; D006369:Heligmosomatoidea / Q000378:metabolism*; D008297:Male; D051379:Mice; D009351:Nematospiroides dubius / Q000201:enzymology / Q000378:metabolism*; D010090:Oxidoreductases, O-Demethylating / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D013053:Spectrophotometry; D013347:Subcellular Fractions / Q000201:enzymology / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism
D003573:Cytochrome b Group; D015262:Xenobiotics; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; C026048:4-nitroanisole O-demethylase; D010090:Oxidoreductases, O-Demethylating; D010105:Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase; D000815:Aniline Hydroxylase