Growth and morphogenesis of mouse prostate epithelial cells in collagen gel matrix culture.
Epithelial cells were isolated from the ventral prostate gland of the mouse after prolonged incubation in a mixture of collagenase, Dispase and hyaluronidase followed by extensive pipetting. The isolated epithelial cells were then embedded in collagen gels. After cultivation in Dulbecco's modified Eagle's medium supplemented with fetal bovine serum, epidermal growth factor, 5 alpha-dihydrotestosterone and cortisol, stimulation of growth and branching morphogenesis of the epithelial cells were observed. Under these culture conditions, growth of contaminating fibroblastic cells was rarely seen. These observations suggest that hormones including androgen directly stimulate the growth and morphogenesis of mouse prostate epithelial cells in culture.
Kusama
Y
Y
Department of Animal Physiology, Faculty of Agriculture, Meiji University, Kanagawa, Japan.
Enami
J
J
Kano
Y
Y
D000728:Androgens / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006728:Hormones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011467:Prostate / Q000166:cytology* / Q000187:drug effects
D000728:Androgens; D006728:Hormones; D004815:Epidermal Growth Factor; D003094:Collagen