Intracellular metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cdr variant.
The regulation of metallothionein (MT) gene expression in a cadmium resistant CHO cell line which overproduces MT was examined in this study. Our results show that MT mRNA levels reach a maximum 24-30 h after a primary zinc exposure and, subsequently, MT mRNA concentrations decline. This decrease in MT mRNA levels can be correlated with the accumulation of metallothionein and decreased rates of zinc and cadmium uptake.
Morris
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S
Department of Biochemistry, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland 21205.
Huang
P C
PC
D000818:Animals; D002104:Cadmium / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae / Q000235:genetics*; D003412:Cricetulus / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D014644:Genetic Variation; D008668:Metallothionein / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology
D012333:RNA, Messenger; D002104:Cadmium; D008668:Metallothionein; D015032:Zinc