Inhibition of inositol 1,4,5-trisphosphate-induced Ca2+ release in permeabilized pancreatic acinar cells by hormonal and phorbol ester pretreatment.
Isolated rabbit pancreatic acinar cells, permeabilized by saponin treatment and incubated in the presence of 0.1 microM free Ca2+, accumulated 0.9-1.5 nmol of Ca2+/mg acinar protein in an energy-dependent pool. Uptake into this pool was not altered by pretreatment of acinar cells with the Ca2+ mobilizing secretagogues carbamylcholine and cholecystokinin-octapeptide or the phorbol ester 12-O-tetradecanoylphorbol 13-acetate, indicating that the Ca2+ pump of the internal Ca2+ store was not affected by prolonged activation of the Ca2+ messenger system. Inositol 1,4,5-trisphosphate (1,4,5-IP3) caused a rapid decrease in Ca2+ content of the internal Ca2+ store. The response was maximal within 30 s following addition of 1,4,5-IP3 and no reuptake of Ca2+ was observed over the next 60 s. Up to 55% of the amount of Ca2+ stored in the energy-dependent pool was 1,4,5-IP3 releasable with an EC50 of 1.0 microM. Pretreatment of acinar cells with carbamylcholine or cholecystokinin-octapeptide significantly reduced the effectivity of 1,4,5-IP3 to release Ca2+ from the internal store. The dose-response curve for 1,4,5-IP3-induced release of actively stored Ca2+ from carbamylcholine-treated acinar cells showed both a rightward shift (EC50 value of 1.7 microM) and a decreased maximal response (maximal release value of 44%), which suggests that the affinity of 1,4,5-IP3 for its receptor as well as the number of 1,4,5-IP3 receptors or 1,4,5-IP3-operated Ca2+ channels was reduced upon prolonged activation of the Ca2+ messenger system. Moreover, analysis of the release values in a Hill plot suggested positive cooperativity for 1,4,5-IP3-induced Ca2+ release from the internal store (n values of 1.3 and 1.7 for saline- and carbamylcholine-treated permeabilized acinar cells, respectively). Pretreatment of acinar cells with 12-O-tetradecanoylphorbol 13-acetate partly mimicked the inhibitory effect of carbamylcholine on 1,4,5-IP3-induced release of actively stored Ca2+ in that the dose-response curve was shifted to the right but the maximal response was not affected, suggesting that the effects of carbamylcholine were at least in part mediated by protein kinase C.(ABSTRACT TRUNCATED AT 400 WORDS)
Willems
P H
PH
Department of Biochemistry, University of Nijmegen, The Netherlands.
Van den Broek
B A
BA
Van Os
C H
CH
De Pont
J J
JJ
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011817:Rabbits; D012844:Sincalide / Q000494:pharmacology*; D013403:Sugar Phosphates / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D007295:Inositol Phosphates; D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D013403:Sugar Phosphates; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D015544:Inositol 1,4,5-Trisphosphate; D002217:Carbachol; D012844:Sincalide; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium