Production of interleukin 6 by human spleen cells stimulated with streptococcal preparation OK-432.
The streptococcal preparation OK-432 was tested for the ability to stimulate human spleen leukocytes (SPL) for generation of interleukin 6 (IL-6). When SPL were cultured with OK-432 for 24 h in serum-free T medium, the cell-free supernatant induced production of IgM in the SKW6.CL-4 and IgG in the CESS human B cell line, while no such activity was detected in unstimulated SPL culture. The activity was neutralized by treatment with antiserum directed against B cell stimulatory factor 2 (BSF-2). An optimum production of BSF-2 was observed when SPL were stimulated with 10 micrograms/ml of OK-432. The culture supernatant also induced proliferation of IL-6-dependent murine hybridoma MH-60.BSF2 (hybridoma growth factor; HGF). It is thus evident that the molecule produced by OK-432-activated human SPL is BSF-2/HGF/IL-6. These results indicate that the antitumor agent OK-432 stimulates human spleen cells to produce IL-6.
Fukui
H
H
Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Co. Ltd., Shizuoka, Japan.
Koishihara
Y
Y
Nagamuta
M
M
Mizutani
Y
Y
Uchida
A
A
D001688:Biological Products / Q000494:pharmacology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006133:Growth Substances / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis*; D007962:Leukocytes / Q000166:cytology; D010844:Picibanil / Q000494:pharmacology*; D013154:Spleen / Q000378:metabolism*
D001688:Biological Products; D006133:Growth Substances; D015850:Interleukin-6; D007378:Interleukins; D010844:Picibanil