A single-stranded RNA copy of the Giardia lamblia virus double-stranded RNA genome is present in the infected Giardia lamblia.
An isolate of Giardia lamblia infected with the double-stranded RNA virus (GLV) has two major species of RNA that are not present in an uninfected isolate. One of these species is the previously characterized double-stranded RNA genome of GLV (1). The second species of RNA appears to be a full length copy of one strand of the double-stranded RNA genome. This full length single-stranded RNA is not present in viral particles isolated from the growth medium. The cellular concentration of the single-stranded RNA changes during exponential and stationary phases of cell growth in a fashion consistent with a viral replicative intermediate or mRNA. The single-stranded species does not appear to be polyadenylated.
Furfine
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School of Pharmacy, Department of Pharmaceutical Chemistry, University of California, San Francisco 94143.
White
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Wang
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Wang
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D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005872:Giardia / Q000382:microbiology*; D008969:Molecular Sequence Data; D012328:RNA Viruses / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics* / Q000648:ultrastructure; D012689:Sequence Homology, Nucleic Acid
D012330:RNA, Double-Stranded; D012367:RNA, Viral