[Effects of ethylene oxide on hepatic microsomal cytochrome P-450: an in vitro study].
The effect of ethylene oxide on rat hepatic microsomal cytochrome P-450 was studied in vitro. Cytochrome P-450, but not b5, was decreased by ethanol. When hepatic microsome was bubbled with ethylene oxide gas, cytochrome P-450 content remained unchanged when compared with CO2 bubbling. The addition of NADPH 1 mM also did not change cytochrome P-450 significantly. The excessive high exposure to 500 mM ethylene oxide also did not affect microsomal cytochrome P-450. These results indicate that ethylene oxide does not destroy directly the hepatic microsomal cytochrome P-450 under these conditions.
Fujishiro
K
K
Department of Environmental Toxicology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Mori
K
K
Inoue
N
N
Sakimura
T
T
Jie
R
R
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005027:Ethylene Oxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D003577:Cytochrome P-450 Enzyme System; D005027:Ethylene Oxide