Posterior half amputation of the chick wing bud: the response of the developing vasculature, and subsequent wound healing.
Experimental analyses examining pattern formation in the developing chick limb have concentrated on the skeleton, muscles and nerves, and have rarely considered blood vessels. To investigate the relationship between the vasculature and limb development, posterior amputations were performed on 3.5-4 day chick limb-buds. It has been shown that the removal of the posterior half alters the developmental fate of the anterior tissue: it becomes necrotic and fails to differentiate into the complement of skeletal parts predicted by fate maps. The possibility that this developmental failure results from interference with the future arterial supply was examined by Indian ink injection between 3-48 h after operation. Scanning electron microscopy (SEM) and resin histology were used to examine the wound repair at similar post-operative intervals. Results from the Indian ink injections showed that within 6 h of operation a collateral circulation was established by means of a branch from the truncated primary subclavian artery. The capillary density in the operated limbs appeared normal when compared to the contralateral limb. The results support the view that the poor developmental performance of the anterior half is due to removal of the zone of polarizing activity (ZPA) rather than to experimentally-induced alteration to the vascular supply. Histological and SEM examination of the wound healing process showed that epithelialization of the cut surface occurred within 24 h, and that the peridermal cells of the bilayered ectoderm appeared to initiate the regrowth. The wound site was not visible 48 h after operation, showing that wound healing at these developmental ages occurs quickly, with no scar tissue formation.(ABSTRACT TRUNCATED AT 250 WORDS)
Wilson
D J
DJ
Department of Dental Surgery and Pathology, Queen's University of Belfast, Northern Ireland.
McNeill
J
J
Hinchliffe
J R
JR
D000671:Amputation, Surgical; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D002642:Chick Embryo; D004475:Ectoderm / Q000502:physiology; D006651:Histocytochemistry; D008648:Mesoderm / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D014921:Wings, Animal / Q000098:blood supply* / Q000196:embryology / Q000293:injuries; D014945:Wound Healing