Identification and characterization of 114/A10, an antigen highly expressed on the surface of murine myeloid and erythroid progenitor cells and IL-3-dependent cell lines.
Monoclonal antibody (mAb) 114/A10, raised against the murine bone marrow-derived multipotential hemopoietic progenitor cell line B6SUtA, identifies an antigen highly expressed by various interleukin-3 (IL-3)-dependent cell lines, the myelomonocytic cell line WEHI-3, and a large proportion of primary myeloid and erythroid colony-forming cells. Spleen- and bone marrow-derived 114/A10-positive cells were shown to selectively proliferate in vitro in response to pokeweed mitogen-stimulated spleen cell-conditioned medium or recombinant IL-3. Western blot analysis indicated that the antigen recognized by mAb 114/A10 has a mean relative molecular mass of approximately 150,000, although it is extremely heterogeneous in nature, and differs greatly in size range among different cell lines.
Dougherty
G J
GJ
Terry Fox Laboratory, B.C. Cancer Research Centre, Vancouver, Canada.
Dougherty
S T
ST
Kay
R J
RJ
Landsdorp
P
P
Humphries
R K
RK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002455:Cell Division; D002460:Cell Line; D004815:Epidermal Growth Factor; D004912:Erythrocytes / Q000166:cytology* / Q000276:immunology; D007377:Interleukin-3 / Q000494:pharmacology*; D051379:Mice; D008970:Molecular Weight; D013154:Spleen / Q000166:cytology; D013234:Stem Cells / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007377:Interleukin-3; C063487:Ly64 protein, mouse; D004815:Epidermal Growth Factor