[Apoprotein B: its plasma level and interaction with cultured fibroblasts].
To study the relationship between the ability of apolipoprotein B (apo B) to interact with LDL receptors and its blood concentration, enzyme immunoassay was used to measure serum apo B levels in 51 healthy donors, 84 patients with coronary heart disease (CHD), and 22 newborns. Employing the same serum samples, the binding of apo B to fibroblasts was determined by the enzyme immunoassay modified for cultured cells. The mean values of apo B concentrations were found to be 0.89 +/- 0.25 mg/ml in the donors, 1.29 +/- 0.51 mg/ml in CHD patients, and 0.57 +/- 0.19 mg/ml in the newborns, while the apo B binding to the cells amounted to 108.9 +/- 16.7, 141.5 +/- 43.6, and 55.0 +/- 24.6 ng/mg, respectively. The mean values of serum apo B binding in the healthy donors and CHD patients were shown to correspond to the concentration relationship of pure LDL interaction with LDL receptors. The findings suggest that higher plasma apo B concentrations in CHD patients cannot be explained by lowered ability of serum apolipoproteins B to interact with LDL receptors of the cells.
Anisimova
O Iu
OIu
Preobrazhenskiĭ
S N
SN
Fuki
I V
IV
Eluashvili
I A
IA
Preobrazhenskiĭ
D V
DV
Liakishev
A A
AA
Repin
V S
VS
D000328:Adult; D001055:Apolipoproteins B / Q000097:blood*; D002478:Cells, Cultured; D003327:Coronary Disease / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D008077:Lipoproteins, LDL / Q000097:blood*; D011485:Protein Binding; D011973:Receptors, LDL / Q000378:metabolism*; D012016:Reference Values; D012867:Skin / Q000166:cytology*
D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D011973:Receptors, LDL
Apoprotein B: kontsentratsiia v plazme krovi i vzaimodeĭstvie s fibroblastami v ku'lture.