Resistance to acid of canine kidney (MDCK) and human colonic (T84) and ileo-caecal (HCT-8) adenocarcinoma epithelial cell monolayers in vitro.
Monolayers of canine kidney (MDCK) and human lower intestinal (T84 and HCT-8) cell lines generated significant transepithelial electrical resistance (700-5000 omega cm2). Electrical integrity was maintained upon acidification of the apical and/or basolateral surfaces to pH 3.0, and this was associated with increased transepithelial electrical resistance, and generation of a potential difference at pH less than 4.5. These results indicate that resistance to acid is a general phenomenon of epithelial layers, and that monolayers of epithelial cells, including those of human origin, are a homogeneous and simple model for studying epithelial barrier function in vitro.
Chan
A B
AB
Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School.
Allen
C N
CN
Simmons
N L
NL
Parsons
M E
ME
Hirst
B H
BH
D000230:Adenocarcinoma / Q000503:physiopathology*; D000818:Animals; D002430:Cecal Neoplasms / Q000503:physiopathology*; D002460:Cell Line; D002478:Cells, Cultured; D003106:Colon / Q000502:physiology*; D004285:Dogs; D004553:Electric Conductivity; D004847:Epithelial Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000502:physiology*; D008564:Membrane Potentials