title: Tumor cell heterogeneity in multiple myeloma: antigenic, morphologic, and functional studies of cells from blood and bone marrow.
abstract: Tumor cells from six patients with immunoglobulin G (IgG) multiple myeloma were analyzed for surface antigens, cytoplasmic paraprotein, morphology, and response to various culture conditions. The tumor marker was the paraprotein idiotype. Low numbers of tumor cells were found in the blood of most of the patients. In some patients, the circulating tumor cells were solely B lymphocytes, whereas in other patients, they were lymphoid, lymphoplasmacytoid, and plasmacytoid. Dual surface antigen analysis of blood and bone marrow cells confirmed that the tumor may be composed of a spectrum of cell types. Thus, cells may range from surface-idiotype+,CD19+,CD20+, PCA-1-,cytoplasmic-idiotype- lymphocytes, to CD19-,PCA-1+,cytoplasmic-idiotype+ plasma cells that are surface-idiotype- or weakly surface-idiotype+. In one patient, some of the tumor cells co-expressed surface idiotype and CD10. The tumor B lymphocytes were activated in vitro to synthesize paraprotein by pokeweed mitogen (PWM), and by low molecular weight B cell growth factor (BCGF). In contrast, spontaneous synthesis of paraprotein by more mature tumor cells was inhibited by agents that also inhibit nonmyeloma plasma cells. These agents included PWM, gamma interferon, and phorbol ester. The results demonstrate that in multiple myeloma there exist different tumor cell types that are similar, by a variety of criteria, to normal B lineage cells at different stages of differentiation. Thus, further evidence is provided for the hypothesis of myeloma cell differentiation.
authors: Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, Australia.
authors: M A
authors: MA
authors: King
authors: D S
authors: DS
authors: Nelson
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology; D001853:Bone Marrow / Q000032:analysis / Q000473:pathology*; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic / Q000032:analysis / Q000473:pathology*; D003593:Cytoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D009101:Multiple Myeloma / Q000032:analysis / Q000097:blood* / Q000473:pathology; D010266:Paraproteins / Q000032:analysis; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012397:Rosette Formation; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007130:Immunoglobulin Idiotypes; D010266:Paraproteins; D011947:Receptors, Antigen, B-Cell