Biological and molecular characterization of human immunodeficiency virus (HIV-1BR) from the brain of a patient with progressive dementia.
HIV-1BR was isolated from the autopsied brain tissue of a 57-year-old man who died of progressive dementing illness. This virus was shown to be HIV-1 by hybridization to HIV-specific DNA probes. The expression of viral proteins as tested by radioimmunoprecipitation assay revealed the presence of HIV-1-specific proteins. HIV-1BR replicated in cultures of CD4+ T-lymphoid cells and induced cytopathic effects in these cells. HIV-1BR also replicated in monocytoid cell lines. The genetic nature of this isolate was determined by molecular cloning and sequencing of the 3'-half of the genome. DNA sequence information established that HIV-1BR is a unique HIV-1 isolate. A stretch of approximately 30 bases in the nef gene of HIV-1BR was found duplicated when compared with the other sequenced HIV-1 genomes. The functional significance of this duplication remains to be determined.
Anand
R
R
Biochemical Genetics Branch, National Institute of Mental Health, Washington, D.C. 20032.
Thayer
R
R
Srinivasan
A
A
Nayyar
S
S
Gardner
M
M
Luciw
P
P
Dandekar
S
S
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000382:microbiology* / Q000473:pathology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics*; D003704:Dementia / Q000209:etiology / Q000382:microbiology*; D015497:HIV-1 / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D011233:Precipitin Tests; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins