[Isoelectric focusing and electroblotting of human vitreous body proteins].
Due to its high resolution and reproducibility, isoelectric focusing with a highly sensitive silver stain, in combination with electroblotting and immunodetection, may be a valuable technique to provide detailed mapping of the protein pattern of the human vitreous. By means of this technique, the presence of albumin, alpha 1-antitrypsin, alpha 1-acid glycoprotein, and transferrin in the normal human vitreous was established. Our results indicate that the differences between vitreous protein und serum protein are more of a quantitative than a qualitative nature. Preliminary studies on pathological vitreous samples obtained during vitrectomy indicate that a substantial amount of the intravitreal protein in PVR as well as in proliferative diabetic retinopathy is plasma-derived. The vitreous from eyes with a macular pucker shows a pattern of protein bands that is reminiscent of normal vitreous.
Moter
H
H
Wiedemann
P
P
Weller
M
M
Heimann
K
K
D005136:Eye Proteins / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D014822:Vitreous Body / Q000032:analysis*
D005136:Eye Proteins
Isoelektrische Fokussierung und Elektroblotting von menschlichen Glaskörperproteinen.