A method of isolation of apical membranous sheets from frog urinary bladder epithelium by stripping with gelatin.
We have developed a technique for recovering apical membranous sheets from amphibian urinary bladders by gelatin stripping. The tissue is mounted on a lucite support and the apical surface is first stuck onto a gelatin-coated glass slide at 30 degrees C. This sandwich is then chilled on ice and the bladder is pulled away from the slide. Preliminary results indicate that this simple technique could be used to remove membranous apical sheets of various sizes, almost devoid of cytoplasmic contamination and without significant damage to the underlying cell structures. The method could also be adapted to prepare perforated cells and to study the cohesive forces between the different layers of the tissue.
Favard
P
P
Centre de Biologie Cellulaire, CNRS, Ivry-sur-Seine, France.
Favard
N
N
Zhu
Q L
QL
Bourguet
J
J
Lechaire
J P
JP
D000818:Animals; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods; D002462:Cell Membrane / Q000648:ultrastructure*; D004848:Epithelium / Q000648:ultrastructure; D005780:Gelatin; D005898:Glass; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D011893:Rana esculenta; D001743:Urinary Bladder / Q000648:ultrastructure*
D005780:Gelatin