cDNA sequences of two inducible T-cell genes.
We have previously described a set of human T-lymphocyte-specific cDNA clones isolated by a modified differential screening procedure. Apparent full-length cDNAs containing the sequences of 14 of the 16 initial isolates were sequenced and were found to represent five different species of mRNA; three of the five species were identical to previously reported cDNA sequences of preproenkephalin, T-cell-replacing factor, and a serine esterase, respectively. The other two species, 4-1BB and L2G25B, were inducible sequences found in mRNA from both a cytolytic T-lymphocyte and a helper T-lymphocyte clone and were not previously described in T-cell mRNA; these mRNA sequences encode peptides of 256 and 92 amino acids, respectively. Both peptides contain putative leader sequences. The protein encoded by 4-1BB also has a potential membrane anchor segment and other features also seen in known receptor proteins.
Kwon
B S
BS
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46223.
Weissman
S M
SM
D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002460:Cell Line; D004247:DNA / Q000235:genetics*; D004745:Enkephalins / Q000235:genetics; D004950:Esterases / Q000235:genetics; D006801:Humans; D015848:Interleukin-5; D007378:Interleukins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D004745:Enkephalins; D015848:Interleukin-5; D007378:Interleukins; D011498:Protein Precursors; D012333:RNA, Messenger; D004247:DNA; C033660:preproenkephalin; D004950:Esterases; C022635:serine esterase