title: Revised amino acid sequence of pancreatic spasmolytic polypeptide exhibits greater similarity with an inducible pS2 peptide found in a human breast cancer cell line.
abstract: The published amino acid sequence of pancreatic spasmolytic polypeptide (Thim, L., Thomsen, J., Christensen, M. and Jørgensen, K.H. et al. (1985) Biochim. Biophys. Acta 827, 410-418) has been checked by a combination of mass spectroscopy and Edman degradation. The pyroglutamyl blocking group was positively identified, and residue assignments at four positions were corrected: Lys48 (not Ser), Ser63 (not Lys), Cys68 (not Ser) and Ser74 (not Cys). The revised sequence exhibits greater similarity with pS2 peptide, a 60 residue polypeptide which is induced by oestrogen in the human breast cancer cell line MCF-7 and found in malignant but not in non-malignant breast tissue.
authors: Département de Biochimie Médicale, C.M.U. Geneva, Switzerland.
authors: K
authors: K
authors: Rose
authors: L A
authors: LA
authors: Savoy
authors: L
authors: L
authors: Thim
authors: M
authors: M
authors: Christensen
authors: K H
authors: KH
authors: Jørgensen
mesh_terms: D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000032:analysis*; D002268:Carboxypeptidases; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010276:Parasympatholytics; D010446:Peptide Fragments; D010455:Peptides; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014357:Trypsin; D014407:Tumor Cells, Cultured
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010276:Parasympatholytics; D010446:Peptide Fragments; D010455:Peptides; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; C034407:pancreatic spasmolytic polypeptide; D002268:Carboxypeptidases; D014357:Trypsin