title: Lymphocyte alterations in zinc-deficient calves with lethal trait A46.
abstract: Lymphocyte numbers and activities were evaluated at 2, 4, 8 and 12 weeks of age in two calves with lethal trait A46 (A46), a genetic disorder affecting intestinal zinc absorption. Plasma zinc concentrations declined to subnormal by 3 weeks of age, after which anorexia, diarrhea, alopecia and hyperkeratosis occurred. Lymphocyte response to phytohemagglutinin-P (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM) stimulation was variably reduced. CD4+ T-lymphocytes were subnormal on at least one observation period following onset of zinc deficiency, and relative numbers of B lymphocytes were decreased at 8 weeks. Secondary antibody responses to bacteriophage phi X 174 were significantly reduced. The results demonstrate that calves homozygous for the A46 trait have normal numbers of functional lymphocyte subpopulations at birth, and that the activity of their lymphocytes is altered once the calves become zinc deficient.
authors: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
authors: L E
authors: LE
authors: Perryman
authors: D R
authors: DR
authors: Leach
authors: W C
authors: WC
authors: Davis
authors: W D
authors: WD
authors: Mickelsen
authors: S R
authors: SR
authors: Heller
authors: H D
authors: HD
authors: Ochs
authors: J A
authors: JA
authors: Ellis
authors: E
authors: E
authors: Brummerstedt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D001435:Bacteriophages / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000235:genetics / Q000276:immunology*; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D005804:Genes, Lethal; D006720:Homozygote; D007408:Intestinal Absorption / Q000235:genetics; D037102:Lectins / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D008297:Male; D015032:Zinc / Q000172:deficiency*
chemical_list: D000911:Antibodies, Monoclonal; D037102:Lectins; D015032:Zinc