Effect of steroid hormones on human colorectal adenocarcinoma xenografts, of known steroid-receptor status, in nude mice.
The effect of hormone therapy on the growth of human colonic adenocarcinoma xenografts in nude mice was evaluated. Primary xeno-transplantation for ten different human colorectal adenocarcinomas into nude mice yielded a tumour take of 50%. One of these host tumours was found to contain androgen receptors (8 fmol/mg cytosol protein; Kd 0.73 x 10(-9) M), which were maintained in the xenograft at the third and ninth passages, but not expressed at the tenth and twelfth passages. The host tumour and its xenograft did not express either oestrogen or progesterone receptors. Administration of dihydrotestosterone led to inhibition of xenograft growth at the ninth passage compared with untreated controls (P less than 0.05), but had no effect on xenograft growth at the tenth and twelfth passages when androgen receptors were absent. Stilboestrol and progesterone failed to influence xenograft growth. In conclusion, dihydrotestosterone administration led to inhibition of xenograft growth only in the presence of androgen receptor, suggesting that some colorectal cancers might be considered steroid-hormone-sensitive tumours.
Stebbings
W S
WS
Professional Surgical Unit, St. Bartholomew's Hospital, London, U.K.
Vinson
G P
GP
Farthing
M J
MJ
Balkwill
F
F
Wood
R F
RF
D000230:Adenocarcinoma / Q000032:analysis / Q000473:pathology*; D000328:Adult; D000368:Aged; D000818:Animals; D015179:Colorectal Neoplasms / Q000032:analysis / Q000473:pathology*; D004054:Diethylstilbestrol / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology; D005260:Female; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011374:Progesterone / Q000494:pharmacology; D011944:Receptors, Androgen / Q000032:analysis; D014183:Transplantation, Heterologous
D012739:Gonadal Steroid Hormones; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D011374:Progesterone; D004054:Diethylstilbestrol