Murine transforming growth factor-beta 2 cDNA sequence and expression in adult tissues and embryos.
Murine transforming growth factor-beta 2 (TGF-beta 2) cDNAs were isolated from cDNA libraries derived from a differentiated murine embryonic carcinoma cell line, PCC3. The composite cDNA sequence is 4267 nucleotides long, including a 1217 nucleotides 5'-untranslated sequence, and encodes a murine TGF-beta 2 precursor of 414 amino acids with 96% identity to its human counterpart. Several consensus polyadenylation sequences are present in the 1807 nucleotides 3'-untranslated sequence. Five TGF-beta 2 mRNA species are observed in the developing mouse fetus and they show different patterns of expression during development. TGF-beta 2 mRNA expression was also examined in adult mouse tissues, in which four of the five RNA species were observed. TGF-beta 2 mRNAs were present in all adult mouse tissues examined, except liver, and was most abundant in placenta, the male submaxillary gland and lung. The patterns of expression suggest a physiological role for TGF-beta 2 both in embryonic development and in the maintenance of adult tissues.
Miller
D A
DA
Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Lee
A
A
Pelton
R W
RW
Chen
E Y
EY
Moses
H L
HL
Derynck
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015342:DNA Probes / Q000302:isolation & purification; D005314:Embryonic and Fetal Development; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D016212:Transforming Growth Factor beta; D015291:Transforming Growth Factors / Q000378:metabolism*
D015342:DNA Probes; D011498:Protein Precursors; D011506:Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D015291:Transforming Growth Factors