title: Cytotoxic activity of various LAK cells against autologous lung cancer, K-562 and Daudi cells.
abstract: Comparative cytotoxic activity and specificity studies of lymphokine activated killer (LAK) cells from different sources and preparations were carried out. Cytotoxic studies of lymphocytes from regional lymph nodes (Rn-LAK), peripheral blood (Pb-LAK) and those stimulated by autologous tumor cells and then activated by recombinant interleukine 2 (St-LAK) against autologous lung cancer cells, were conducted using the 51Cr-releasing test. The cytotoxic activities of these three LAK cells against autologous fibroblasts, allogeneic tumor cells, K-562 and Daudi cells were also compared. A time course experiment disclosed that the cytotoxic activity of Pb-LAK cells against autologous tumor cells reached a maximum on day 3 and declined shortly after the peak while that of Rn-LAK cells gradually increased to a maximum on day 7 and remained at a high titer for at least two weeks. The cytotoxic activity of Rn-LAK cells was significantly higher than that of Pb-LAK cells in seven out of 10 individual experiments and that of stimulated LAK (St-LAK) cells was higher than the cytotoxic activities of the other LAK cells in eight out of nine experiments. A specificity study showed that Rn-LAK cells possessed a higher cytotoxic activity against autologous tumor cells and a lower cytotoxic activity against autologous fibroblasts and allogeneic tumor cells than Pb-LAK cells. A comparative study of cytotoxic activities against K-562, Daudi cells and autologous tumor cells between St- and Rn-LAK cells demonstrated that although there was no difference in natural killer activity, cytotoxic activity against autologous tumor cells as well as the LAK activity of St-LAK cells were higher than that of Rn-LAK cells. These results indicate that the activities and specificities of LAK cells differ according to their source and preparation.
authors: Department of Surgery, School of Medicine, Chiba University.
authors: H
authors: H
authors: Kimura
authors: Y
authors: Y
authors: Yamaguchi
mesh_terms: D000368:Aged; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007951:Leukemia, Myeloid / Q000276:immunology* / Q000473:pathology; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008198:Lymph Nodes / Q000166:cytology; D008223:Lymphoma / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured / Q000276:immunology