Prevalence of human immunodeficiency virus type 1 DNA in hemophilic men and their sex partners. Hemophilia-AIDS Collaborative Study Group.
Polymerase chain reaction (PCR) was used to detect human immunodeficiency virus (HIV)-1 DNA in peripheral blood mononuclear cells to assess in hemophilic men whether any were HIV-seropositive but uninfected or seronegative but infected and in seronegative sex partners of seropositive hemophilic men whether any were infected. Of 40 seropositive men, 38 (95%) were PCR-positive; one was PCR-indeterminate and one PCR-negative. None of 41 seronegative men who used only donor-screened, virus-inactivated coagulation factor products were PCR-positive. However, two of six who received noninactivated products were PCR-positive; one had low T-helper cell counts and died of unrelated causes and the other had seroconverted 11 mo later. PCR with a second primer pair also detected HIV-1 DNA in these two men. None of 25 seronegative female sex partners of seropositive men, including six men with AIDS and seven with AIDS-related symptoms, were PCR-positive. These data suggest that most seropositive hemophilic men are HIV-infected; whether some are infected with defective virus remains to be resolved as does the infection status of seropositive PCR-negative men. Identification of two seronegative PCR-positive men supports the possibility that HIV-1 DNA can be detected before seroconversion.
Jason
J
J
Center for Infectious Diseases, Atlanta, Georgia.
Ou
C Y
CY
Moore
J L
JL
Lawrence
D N
DN
Schochetman
G
G
Evatt
B L
BL
D004279:DNA, Viral / Q000032:analysis; D006679:HIV Seropositivity / Q000382:microbiology*; D015497:HIV-1 / Q000032:analysis* / Q000235:genetics; D006467:Hemophilia A / Q000382:microbiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008137:Longitudinal Studies; D008297:Male; D016133:Polymerase Chain Reaction; D012747:Sexual Partners
D004279:DNA, Viral