Microsomal membrane fluidity and phosphatidylcholine synthesis in rabbit lung under high oxygen tension.
Phosphatidylcholine metabolism and membrane fluidity were studied in microsomes isolated from rabbit lung, which had been exposed to high oxygen tension for 30 min. In these microsomes the incorporation of [3H]-palmitate into phosphatidylcholine increased whereas the incorporation of [14C]-glycerol and [14C]-choline from CDP-[methyl-14C]-choline remained unchanged in comparison to the control microsomes. The enhanced [3H]-palmitate incorporation may be explained by an increase of the specific activity of acyl-CoA:lysophosphatidylcholine acyltransferase which was measured in microsomes from hyperoxic lung. Although microsomal parameters influencing membrane fluidity, such as the cholesterol/phospholipid molar ratio, unsaturation degree of phospholipid acyl chains and lipid/protein ratio, are altered after oxygen treatment in vivo, no change of fluorescence polarization (PDPH) and lipid structural order parameter (SDPH) could be measured. Probably, the membrane maintains its fluidity by counteracting effects on different factors on which the fluidity depends.
Casals
C
C
Department of Biochemistry, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
Herrera
L
L
Gasset
M
M
Garcia-Barreno
P
P
Municio
A M
AM
D000215:Acylation; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism; D005454:Fluorescence Polarization; D007425:Intracellular Membranes / Q000187:drug effects*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000633:toxicity*; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D011817:Rabbits
D005227:Fatty Acids; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010100:Oxygen