Rapid detection of Streptococcus suis serotype 2 in weaned pigs.
A survey to detect Streptococcus suis serotype 2 in 1,716 weaned pigs was done in Quebec. Forty-nine sow herds were included in this survey: in 26 herds, S suis serotype 2 had been isolated during the preceding 12 months and in 23 herds (control), the organism had not been detected during a previous study. Swab specimens of the nasal cavity and tonsils of pigs were obtained for bacteriologic culture, and S suis serotype 2 was easily detected by the use of brain-heart infusion agar containing a Streptococcus-selective supplement and 5% goat antiserum raised against S suis serotype 2. After measurement of the diameter of the precipitation zone of 539 isolates, a slide agglutination test was performed to identify the S suis serotype 2 isolates. The mean precipitation zone diameter obtained for group S suis serotype 2 was larger (P less than 0.001) than that for the group designated as "others". With slide agglutination test results as reference and on the basis of discriminant analysis to stimulate detection of S suis serotype 2, 93.1% of all isolates were correctly classified, using the precipitation zone diameter as unique classification criterion. Relative specificity was 94.5% and relative sensitivity was 88.7%. Use of the precipitation zone diameter on a quantitative basis led to the proposal of a simple and reliable technique to screen swine herds for S suis serotype 2 in weaned pigs. Nasal and tonsillar swab specimens were obtained and analyzed concurrently for S suis serotype 2. The organism was found in both sites in only 20.4% of 103 carrier pigs.(ABSTRACT TRUNCATED AT 250 WORDS)
Moreau
A
A
Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Qc, Canada.
Higgins
R
R
Bigras-Poulin
M
M
Nadeau
M
M
D000372:Agglutination Tests; D000818:Animals; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D003470:Culture Media; D005260:Female; D007106:Immune Sera; D009296:Nasal Cavity / Q000382:microbiology; D014066:Palatine Tonsil / Q000382:microbiology; D011233:Precipitin Tests; D011237:Predictive Value of Tests; D011792:Quebec; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000302:isolation & purification*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*; D014886:Weaning
D003470:Culture Media; D007106:Immune Sera