On the mechanism of peroxyoxalate chemiluminescence. Quenched chemiluminescence as a detection method in HPLC.
Several analytes such as the inorganic anions bromide, iodide, sulphite and nitrite and organic compounds as substituted anilines and sulphur compounds cause quenching of peroxyoxalate chemiluminescence. A detection method for liquid chromatography based on the quenching phenomenon has been developed. It makes use of an immobilized luminophore, i.e. 3-aminofluoranthene covalently bound via an alkyl-spacer on controlled pore glass, packed in the detector cell. The mechanism behind the quenching has been elucidated by investigating the roles of luminophores (both in the liquid and in solid state) and oxalates in peroxylate CL with respect to quenchers. Most probably the quencher destroys the radical ion pair produced after electron transfer in the last stage of the CIEEL reaction scheme, thus preventing the formation of electronically excited luminophore.
Gooijer
C
C
Free University, Department of General and Analytical Chemistry, Amsterdam, The Netherlands.
van Zoonen
P
P
Velthorst
N H
NH
Frei
R W
RW
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004579:Electron Transport; D005456:Fluorescent Dyes; D005609:Free Radicals; D008163:Luminescent Measurements; D010070:Oxalates
D005456:Fluorescent Dyes; D005609:Free Radicals; D010070:Oxalates; C044622:peroxyoxalate