Rapid assessment of the antitetanus immune status of a subject using Dot-ELISA.
We describe a Dot-ELISA system for the rapid, specific and reliable assessment of a subject's antitetanus immune status. The conditions for Dot-ELISA were selected to give positive reactions only for sera with antitetanus antibody titers equal to or higher than 0.06 I.U./ml, considered to represent protection when using "in vitro" methods. This Dot-ELISA method could advantageously be applied to the monitoring of vaccination campaigns, as well as for assessing the antitetanus immune status of the wounded.
Mastroeni
P
P
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Messina, Italy.
Leonardi
M S
MS
Gazzara
D
D
Bizzini
B
B
D000907:Antibodies, Bacterial / Q000032:analysis*; D004168:Diphtheria Toxoid / Q000276:immunology*; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004338:Drug Combinations / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010567:Pertussis Vaccine / Q000276:immunology*; D013742:Tetanus / Q000276:immunology* / Q000517:prevention & control; D013745:Tetanus Toxoid / Q000276:immunology*
D000907:Antibodies, Bacterial; D004168:Diphtheria Toxoid; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D004338:Drug Combinations; D010567:Pertussis Vaccine; D013745:Tetanus Toxoid