Detection of cell-affecting agents with a silicon biosensor.
Cellular metabolism is affected by many factors in a cell's environment. Given a sufficiently sensitive method for measuring cellular metabolic rates, it should be possible to detect a wide variety of chemical and physical stimuli. A biosensor has been constructed in which living cells are confined to a flow chamber in which a potentiometric sensor continually measures the rate of production of acidic metabolites. Exploratory studies demonstrate several applications of the device in basic science and technology.
Parce
J W
JW
Molecular Devices Corporation, Menlo Park, CA 94025.
Owicki
J C
JC
Kercso
K M
KM
Sigal
G B
GB
Wada
H G
HG
Muir
V C
VC
Bousse
L J
LJ
Ross
K L
KL
Sikic
B I
BI
McConnell
H M
HM
D015374:Biosensing Techniques; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002477:Cells / Q000378:metabolism*; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D010101:Oxygen Consumption; D012825:Silicon
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D004815:Epidermal Growth Factor; D012825:Silicon