Photoaffinity labelling of macrophages and B-lymphocytes using 125I-labelled aryl-azide derivatives of muramyldipeptide.
Two 125I-labelled aryl-azide derivatives of MDP have been synthesized. Photoaffinity labelling experiments demonstrated cell surface binding sites for muramylpeptides on activated B-lymphocytes and intracellular muramylpeptides binding protein(s) of 40-45 kD in rat alveolar macrophages.
Tenu
J P
JP
CNRS URA 1116, Institut de Biochimie, Université Paris-Sud, Orsay, France.
Adam
A
A
Souvannavong
V
V
Yapo
A
A
Petit
J F
JF
Douglas
K
K
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives*; D000345:Affinity Labels; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D005260:Female; D007457:Iodine Radioisotopes; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010777:Photochemistry; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D000345:Affinity Labels; D007457:Iodine Radioisotopes; D000119:Acetylmuramyl-Alanyl-Isoglutamine