Effect of calcium ion on the interaction of aldolase with rabbit muscle myofibrils.
A partition equilibrium study has shown calcium ion to be a noncompetitive inhibitor of aldolase adsorption by rabbit muscle myofibrils. This inhibition is interpreted quantitatively in terms of a 10-fold decrease in the intrinsic association constant for the aldolase-myofibril interaction upon Ca2+ binding to either or both of the low-affinity troponin sites associated with regulation of muscle contraction.
Harris
S J
SJ
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Winzor
D J
DJ
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002413:Cations, Divalent; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009132:Muscles / Q000201:enzymology / Q000648:ultrastructure; D009210:Myofibrils / Q000201:enzymology*; D011817:Rabbits; D014336:Troponin / Q000378:metabolism
D002413:Cations, Divalent; D014336:Troponin; D005634:Fructose-Bisphosphate Aldolase; D002118:Calcium