Effect of incorporation of serum from dogs with renal impairment on canine erythroid bone marrow cultures.
Serum from dogs with surgically induced renal impairment was incorporated into the medium for erythroid bone marrow cultures. A significant correlation was found between serum activities of erythropoietin and numbers of erythroid colony-forming units grown in culture. Serum creatinine concentrations had no correlation, and serum parathyroid hormone activities had a negative correlation with numbers of erythroid colony-forming units that was below the level of significance. Purified 1-84 parathyroid hormone added to bone marrow cultures was found to be stimulatory to erythroid colony-forming unit growth in higher concentrations, but decreased the number of burst-forming units. Unmeasured substances in the canine serum appeared to have a greater effect on the canine erythroid bone marrow cultures than did creatinine or parathyroid hormone values.
Petrites-Murphy
M B
MB
Department of Veterinary Pathology, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Pierce
K R
KR
Fisher
J W
JW
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003404:Creatinine / Q000097:blood; D003470:Culture Media; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D015672:Erythroid Precursor Cells / Q000166:cytology*; D004921:Erythropoietin / Q000097:blood; D007674:Kidney Diseases / Q000097:blood / Q000662:veterinary*; D010281:Parathyroid Hormone / Q000097:blood / Q000494:pharmacology
D003470:Culture Media; D010281:Parathyroid Hormone; D004921:Erythropoietin; D003404:Creatinine