Immunoreactive tachykinins, calcitonin gene-related peptide and neuropeptide Y in human synovial fluid from inflamed knee joints.
We have analysed the concentrations of substance P (SP), neurokinin A (NKA), calcitonin gene-related peptide (CGRP) and neuropeptide Y (NPY) in the synovial fluid from 5 patients suffering from arthritis with inflamed knee joints as well as from 5 healthy control subjects with an earlier traumatic meniscal or cruciate ligament injury. Competitive radioimmunoassay was done using antisera SP2 (SP), K12 (NKA), R8 (CGRP), and NPY1 (NPY). No SP-like immunoreactivity (-LI) was detected in any patient. NKA-LI was found in all control patients but in none of the arthritis patients. CGRP-LI was seen in all arthritis patients as well as in 3/5 control patients, a non-significant difference. NPY-LI was found in a significantly higher concentration in the arthritis group vs the control patients. The results support an involvement of neuropeptides in human joint inflammation.
Larsson
J
J
Department of Physiology II, Karolinska Institute, Stockholm, Sweden.
Ekblom
A
A
Henriksson
K
K
Lundeberg
T
T
Theodorsson
E
E
D000328:Adult; D000368:Aged; D001168:Arthritis / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007717:Knee; D008297:Male; D008875:Middle Aged; D009478:Neuropeptide Y / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D013582:Synovial Fluid / Q000378:metabolism*; D015320:Tachykinins / Q000378:metabolism*
D009478:Neuropeptide Y; D009479:Neuropeptides; D015320:Tachykinins; D015740:Calcitonin Gene-Related Peptide