Cadmium-induced synthesis of metallothioneins in human T and B cell purified by a fluorescence activated cell sorter.
Human peripheral blood lymphocytes were reacted with fluorescein-conjugated antibodies specific to T or B cell surface antigen and fractionated with a fluorescence activated cell sorter. The isolated T and B cells were examined for their capacity to synthesize metallothioneins (MTs). Analysis by gel electrophoresis indicated that both T and B cells were able to produce MTs in a Cd2+-inducible manner, suggesting that both cells types have a mechanism of protection against Cd2+ toxicity.
Yamada
H
H
National Institute of Industrial Health, Kawasaki, Japan.
Minoshima
S
S
Koizumi
S
S
Kimura
M
M
Shimizu
N
N
D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002104:Cadmium / Q000494:pharmacology*; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis* / Q000097:blood; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D002104:Cadmium; D008668:Metallothionein