title: Immediate decrease in antigen-presenting function and delayed enhancement of interleukin-1 production in human epidermal cells after in vivo UVB irradiation.
abstract: Human skin was irradiated in vivo with a single UVB dose (100 mJ/cm2 or 200 mJ/cm2) to examine simultaneously the antigen-presenting function and interleukin-1 (IL-1) production capacity of irradiated epidermal cells (EC). Suction blisters were produced on irradiated areas on days 0, 3 and 7 after UVB. Irradiated EC were harvested and co-cultured with autologous T lymphocytes in the presence of antigens (PPD, HSV) or mitogen (ConA). Culture supernatants were tested for IL-1 activity using the thymocyte comitogenity assay. We found that a single 200 mJ/cm2 dose of UVB caused an immediate suppression of the antigen-presenting function of EC, but no alteration in their IL-1 production capacity or surface marker expression (ATPase, CDI). PPD- and HSV-induced lymphocyte proliferation was decreased 70-80% and ConA-driven proliferation 30% when compared to non-irradiated EC. However, this suppression was restored on days 3 and 7 after UVB irradiation, this being coexistent with an increased capacity of EC to produce IL-1. It remains to be elucidated whether the immediate UVB-induced photoimmunosuppression observed in the present study is due to inhibitory mediators or impaired membrane function of EC or both.
authors: Department of Biomedical, University of Tampere, Finland.
authors: L
authors: L
authors: Räsänen
authors: T
authors: T
authors: Reunala
authors: M
authors: M
authors: Lehto
authors: C
authors: C
authors: Jansén
authors: I
authors: I
authors: Rantala
authors: P
authors: P
authors: Leinikki
mesh_terms: D000328:Adult; D000938:Antigen-Presenting Cells / Q000528:radiation effects; D002452:Cell Count; D004307:Dose-Response Relationship, Radiation; D004817:Epidermis / Q000276:immunology; D006801:Humans; D007165:Immunosuppression Therapy; D007375:Interleukin-1 / Q000096:biosynthesis*; D007801:Langerhans Cells / Q000276:immunology; D008213:Lymphocyte Activation / Q000528:radiation effects; D008875:Middle Aged; D012867:Skin / Q000276:immunology / Q000528:radiation effects*; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D007375:Interleukin-1