Distinct effects of aminopterin on stimulation of T and B lymphocytes with mitogen.
Aminopterin, a folate antagonist, added to cultures of mouse spleen cells stimulated with T-cell mitogens markedly inhibited, in a dose-dependent manner, [3H]thymidine incorporation into the cells. Contrary to this inhibitory effect aminopterin added at the same concentrations to cell cultures stimulated with B-cell mitogens significantly increased [3H]thymidine incorporation. The inhibitory effect of aminopterin on T-cell activation was completely reversed by adding hypoxanthine to the cultures. Analysis of the mechanism of aminopterin action showed direct, mutually opposite, effects of this drug on T- and B-cell populations. The results thus not only demonstrate that polyclonally activated T and B lymphocytes differ in their sensitivity to the toxic action of aminopterin, but simultaneously support early findings suggesting differences in nucleic acid metabolic pathways between T- and B-cell populations.
Holán
V
V
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Stĕdra
J
J
D000630:Aminopterin / Q000494:pharmacology*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D066298:In Vitro Techniques; D007378:Interleukins / Q000502:physiology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens; D008938:Mitosis / Q000187:drug effects; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D007042:Hypoxanthines; D007378:Interleukins; D008934:Mitogens; D019271:Hypoxanthine; D000630:Aminopterin; D013936:Thymidine