title: An enzymatic method for oxalate automated with the Cobas Fara centrifugal analyzer.
abstract: Measurement of oxalate in urine has been automated for use with the Cobas Fara centrifugal analyzer. No sample pretreatment other than (a critical) pH adjustment is required. Between-run CVs were less than 4%. Results were linearly related to oxalate concentration to 1000 mumol/L. Ascorbic acid ingestion, up to 5 g of ascorbate daily, caused no demonstrable interference with the assay. This practical, automated method for assaying urinary oxalate is substantially faster than other chemical or enzymatic methods.
authors: Department of Clinical Biochemistry, Toronto General Hospital, Ontario, Canada.
authors: L C
authors: LC
authors: Allen
authors: L
authors: L
authors: Kadijevic
authors: A D
authors: AD
authors: Romaschin
mesh_terms: D000445:Aldehyde Oxidoreductases; D001322:Autoanalysis / Q000295:instrumentation; D002262:Carboxy-Lyases; D002498:Centrifugation / Q000295:instrumentation; D005560:Formate Dehydrogenases; D006801:Humans; D010070:Oxalates / Q000592:standards / Q000652:urine*; D011933:Reagent Kits, Diagnostic
chemical_list: D010070:Oxalates; D011933:Reagent Kits, Diagnostic; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases; D002262:Carboxy-Lyases; C021915:oxalate decarboxylase