title: Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3.
abstract: A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12,000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5'- and a longer 3'-noncoding region. The deduced protein sequence was verified by partial peptide sequencing.
authors: Institut für Biochemie, Technische Hochschule Darmstadt, FRG.
authors: M
authors: M
authors: Kriechbaum
authors: H J
authors: HJ
authors: Heilmann
authors: F J
authors: FJ
authors: Wientjes
authors: M
authors: M
authors: Hahn
authors: K D
authors: KD
authors: Jany
authors: H G
authors: HG
authors: Gassen
authors: F
authors: F
authors: Sharif
authors: G
authors: G
authors: Alaeddinoglu
mesh_terms: D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005800:Genes, Fungal; D005949:Glucose Oxidase / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis
chemical_list: D009841:Oligonucleotides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D004247:DNA; D005949:Glucose Oxidase