Systematic isolation and identification of membrane lipid oxidation products.
This report discusses the analytical procedure by which it is possible to isolate and identify the oxidation products of cellular and subcellular membrane lipids. The key point of this procedure is the method used for the transmethylation of the lipid material isolated from the tissues. In effect, both the conversion of the glycerides into methyl esters and the reduction of the hydroperoxyl groups into the corresponding hydroxyl groups is performed in one step, without breaking any oxirane rings that may be present. The methyl esters containing functional groups introduced by oxidative processes are separated from the non-modified ones by preparative TLC and are identified by GLC and GC-MS.
Piretti
M V
MV
Dipartimento di Biochimica, Sezione di Biochimica Veterinaria, Università di Bologna, Italy.
Pagliuca
G
G
D002855:Chromatography, Thin Layer; D008563:Membrane Lipids / Q000302:isolation & purification* / Q000378:metabolism; D008722:Methods; D010084:Oxidation-Reduction
D008563:Membrane Lipids