Leucine aminopeptidase activity of polyploid cells after centrifugal elutriation of rat hepatocytes of various ages.
Rat hepatocytes were isolated by the perfusion method, and further separated with centrifugal elutriation into diploid cells and polyploid cells. The ploidy class and amount of polyploid (octaploid) cells increased with increasing flow rate. At higher flow rates of centrifugal elutriation, older rats contained much more octaploid cells than younger rats. Activity of acid phosphatase and leucine aminopeptidase increased with increasing flow rate (increasing ploidy class). Protein components of hepatocytes in octaploid cells were different from those of tetraploid cells. From these results, it is suggested that the function of the higher ploidy class hepatocytes is greater than that of lower ploidy class hepatocytes.
Nakano
M
M
Institute for Medical Science of Aging, Aichi Medical University, Japan.
Mizuno
T
T
D000135:Acid Phosphatase / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002498:Centrifugation; D007931:Leucyl Aminopeptidase / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D010477:Perfusion; D011123:Polyploidy; D051381:Rats
D000135:Acid Phosphatase; D007931:Leucyl Aminopeptidase