[The effect of phenobarbital and amidopyrine on the rate of decomposition of isoforms of cytochrome P-450 in mouse liver microsomes].
Separation of microsomal proteins in gradient polyacrylamide gel gives 60 protein bands. The molecular mass range of 48-58 kDa corresponding to cytochrome isozymes contains 7 bands for intact mice and 8 bands for phenobarbital-induced mice. Phenobarbital treatment causes both the appearance of a new cytochrome P-450 isozyme with a molecular mass of 56 kDa and the increase in the content of three isozymes with molecular masses of 54, 52.5 and 50 kDa. The half-life time of cytochrome P-450 isozymes in the livers of intact and phenobarbital-induced mice differs from 15 to 42 hours. Phenobarbital induction results in the breakdown acceleration of the isozyme with a molecular mass of 52.5 kDa and the breakdown retardation of the isozyme with a molecular mass of 54 kDa. Aminopyrine injections to phenobarbital-pretreated mice result in the breakdown acceleration of the cytochrome P-450 isozyme with a molecular mass of 56 kDa.
Adrianov
N V
NV
Dovgiĭ
A I
AI
Khaĭlov
P M
PM
D000632:Aminopyrine / Q000494:pharmacology*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006207:Half-Life; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology*
D007527:Isoenzymes; D000632:Aminopyrine; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital
Vliianie fenobarbitala i amidopirina na skorost' raspada izoform tsitokhroma P-450 v mikrosomakh pecheni mysheĭ.