title: Non-additive inheritance of glucose phosphate isomerase activity in mice heterozygous at the Gpi-1s structural locus.
abstract: The activity of blood glucose phosphate isomerase (GPI-1) in mice heterozygous for various alleles at the Gpi-1s structural locus (heterozygotes a/b, a/c and b/c) was significantly higher than expected, on the basis of additive inheritance, from the levels in parental homozygotes. Moreover, the GPI-1 activity was higher in a/b heterozygotes than in either parent (heterosis). Studies of heat stability with kidney homogenates revealed that the relative stabilities of GPI-1 dimers was AA greater than AB greater than BB greater than AC greater than or equal to BC greater than CC. Differences in dimer stabilities in vivo would affect the total GPI-1 levels in heterozygotes and could account for non-additive inheritance but would be insufficient to explain heterosis for GPI-1 activity. Other possible contributing factors include unequal production or stability of monomers, or higher catalytic activity of heterodimers. Monomers could also associate non-randomly but this would not be sufficient to explain heterosis. It is clear that non-additive inheritance patterns may be produced by variants of either structural or regulatory genes.
authors: J D
authors: JD
authors: West
authors: J H
authors: JH
authors: Flockhart
mesh_terms: D000818:Animals; D004795:Enzyme Stability; D005796:Genes; D005838:Genotype; D005956:Glucose-6-Phosphate Isomerase / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D006579:Heterozygote; D006358:Hot Temperature; D007527:Isoenzymes / Q000097:blood / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D007527:Isoenzymes; D005956:Glucose-6-Phosphate Isomerase