title: Effects of oxygen concentration on the metabolism of [U-14C]glucose by mouse morulae and early blastocysts in vitro.
abstract: The utilization of the acid-soluble glycogen pool in pulse-labelled embryos was significantly enhanced during 24- and 48-h chase culture under low oxygen concentrations of 5, 2.5 and 1%. The lower the oxygen tension the greater was the turnover in the pool. The morphological development of embryos was equally as good at very low oxygen concentrations as when embryos were cultured in 5-20% oxygen. Reduction in oxygen concentration enhanced the oxidative utilization of substrate, as measured by rate of carbon dioxide production. The present study could provide an explanation for the discrepancy in glycogen content between mouse blastocysts developing in utero and in vitro and for the reported beneficial effects of low oxygen concentration during development of embryos in culture.
authors: School of Veterinary Studies, Murdoch University, W.A.
authors: N K
authors: NK
authors: Khurana
authors: R G
authors: RG
authors: Wales
mesh_terms: D000818:Animals; D001711:Biotransformation; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D002970:Cleavage Stage, Ovum / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D009028:Morula / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D011247:Pregnancy; D013997:Time Factors
chemical_list: D005947:Glucose; D010100:Oxygen