title: Quantitative analysis of low molecular weight polar compounds by continuous flow liquid secondary ion tandem mass spectrometry.
abstract: Quantitative analyses of low molecular weight (100-200) polar compounds [1-methyl-4-phenylpyridine (MPP+), 2-amino-3-(methylamino)propanoic acid (synonyms, beta-(methyl-amino)-L-alanine or BMAA), and tryptophan] were conducted on a triple-stage quadrupole mass spectrometer configured for continuous flow liquid secondary ion mass spectrometry ionization (CF L-SIMS). It is shown that quantification by CF L-SIMS at subnanogram sensitivity can be precise (correlation coefficients greater than 0.99), accurate, specific, and routine for compounds not measurable by static L-SIMS. Successful analyses, however, are strongly dependent upon the stability of the film formed by the mobile phase on the probe tip. In our system, film stability is affected by mobile phase composition and flow rate, ion source and probe tip temperature, probe-tip and capillary alignment, film thickness, and sample composition.
authors: T C
authors: TC
authors: Wang
authors: M C
authors: MC
authors: Shih
authors: S P
authors: SP
authors: Markey
authors: M W
authors: MW
authors: Duncan
mesh_terms: D015655:1-Methyl-4-phenylpyridinium; D000311:Adrenal Glands / Q000032:analysis*; D000599:Amino Acids, Diamino / Q000032:analysis*; D000818:Animals; D000087522:Cyanobacteria Toxins; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D011726:Pyridinium Compounds / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014364:Tryptophan / Q000032:analysis*
chemical_list: D000599:Amino Acids, Diamino; D000087522:Cyanobacteria Toxins; D011726:Pyridinium Compounds; C001824:beta-N-methylamino-L-alanine; D014364:Tryptophan; D015655:1-Methyl-4-phenylpyridinium