Comparison of tanned cell hemagglutination and counter immunoelectrophoresis test in the detection of antibodies to extractable nuclear antigens (ENA) and to DNA in the systemic lupus erythematosus.
A comparative study of tanned cell hemagglutination (TCH) and counterimmunoelectrophoresis (CIE), two easy and reliable methodes for the routine detection of antibodies against nuclear antigens was performed. Antibodies against ENA, RNA-ase sensitive ENA and DNA antigens were searched in patients affected by systemic lupus erythematosus (SLE). Analysis of the results obtained for a particular antibody using TCH and CIE techniques suggests that both methods should be used for the detection of antibodies to nuclear antigens since in several cases antibodies were detected by one method and not for the other. Besides, the frequency of antibodies to ENA, RNA-ase sensitive ENA and DNA revealed by both techniques is similar to the results reported by others employing laborious tests. TCH and CIE serve as a screen to determine the presence of an antibody system and seems to provide the sensitivity enough as to be used in the smaller routine laboratories that wish to provide services for antibodies to nuclear antigens. When tanned cell hemagglutination was used looking for antibodies to DNA or ENA in the sera of patients affected by SLE it proved to be useful since in samples where antibodies to DNA were absent or at very low titres, antibodies to ENA were present in titres ranging from 1:9 to 1:6 561. The authors think this is of considerable importance since the variety of clinical features seen in different subjects with SLE is often accompanied by different specificities or types and amounts of autoantibodies and that certain combinations of these antibodies coincide with specific clinicopathological abnormalities.
Depiante-Depaoli
M
M
Cátedra de Química Clínica II, Universidad Nacional de Córdoba, Argentina.
Galmarini
M
M
Pesoa
S A
SA
Riera
C M
CM
D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis*; D001773:Blood Cells / Q000276:immunology*; D003378:Counterimmunoelectrophoresis; D004247:DNA / Q000276:immunology*; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D000974:Antibodies, Antinuclear; D004247:DNA