[Osteogenic properties of adhesive cells in Dexter culture of the mouse bone marrow].
Disaggregated cell suspensions obtained by mouse bone marrow fermentative digestion as well as stromal tissue obtained by marrow mild mechanical destruction were explanted. Both methods yield the cultures in which the hematopoiesis duration is comparable with dexter cultures. Adhesive cells from all of these three culture types were resuspended and in the porous gelatin sponges heterotopically transplanted under the kidney capsule of syngenic recipients. In the transplantation site there develops the hemopoietic organ containing reticular stroma, hemopoietic cells, and in most cases the well developed bone tissue. Thus, the adherent layers of mouse bone marrow dexter and similar cultures contain for a long period (not less than 2-3.5 months) the stromal fibroblast population which maintains its osteogenic and hemopoietic microenvironment transfer capacities.
Kuznetsov
S A
SA
Grosheva
A G
AG
Fridenshteĭn
A Ia
AIa
D000818:Animals; D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002448:Cell Adhesion; D046508:Culture Techniques; D005347:Fibroblasts; D006410:Hematopoiesis; D051379:Mice; D008810:Mice, Inbred C57BL; D010012:Osteogenesis; D014185:Transplantation, Isogeneic
Osteogennye svoĭstva adgezivnykh kletok iz deksterovskikh kul'tur kostnogo mozga myshi.