title: Lipid peroxidation and activity of antioxidant enzymes in muscle of the lower leg before and after arterial reconstruction.
abstract: Reperfusion syndrome and lipid peroxidation due to toxic effects of free oxygen radicals might be one pathophysiological cause of the oedema that develops during the first week after a femoro-popliteal reconstruction. This paper reports the activity of the protecting antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase and catalase in gastrocnemic muscle before and after reperfusion of chronically ischaemic legs with comparison to activities in non-ischaemic muscle. Furthermore the susceptibility to lipid peroxidation in the muscle was measured as thiobarbituric acid reactive material (TBAR). The activities of CuZn SOD, Mn SOD and glutathione peroxidase were equal in normally perfused and chronically ischaemic muscle and there was no difference after reperfusion. Muscle catalase activity was low compared to activity of red blood cells and could not be reliably estimated. There was no difference in iron-stimulated lipid peroxidation of ischaemic and non-ischaemic muscle but in reliably estimated. There was no difference in iron-stimulated lipid peroxidation of ischaemic and non-ischaemic muscle but in muscle biopsied 10 min after reperfusion there was a significant increase in production of TBAR indicating an increased susceptibility for lipid peroxidation at this time. The finding is compatible with the occurrence of an oxidant insult on the muscle at reperfusion. Ischaemia--or reperfusion--induced reductions in activity of antioxidant enzymes are however not related to this reaction.
authors: Department of Surgery, Malmö General Hospital, University of Lund, Sweden.
authors: N H
authors: NH
authors: Persson
authors: D
authors: D
authors: Bergqvist
authors: G
authors: G
authors: Fex
authors: S L
authors: SL
authors: Marklund
authors: B
authors: B
authors: Nilsson
authors: R
authors: R
authors: Takolander
mesh_terms: D001161:Arteriosclerosis / Q000201:enzymology / Q000601:surgery; D002374:Catalase / Q000378:metabolism; D005609:Free Radicals; D005979:Glutathione Peroxidase / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007866:Leg / Q000098:blood supply; D015227:Lipid Peroxidation; D009132:Muscles / Q000201:enzymology*; D015424:Reperfusion; D013482:Superoxide Dismutase / Q000378:metabolism; D014656:Vascular Surgical Procedures
chemical_list: D005609:Free Radicals; D006454:Hemoglobins; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase