title: Prostaglandin F2 alpha synthesis and metabolism by luteal phase endometrium in vitro.
abstract: Differences in prostaglandin (PG) F2 alpha synthesis and degradation were sought between early luteal endometrium (histology day 15 to 22, n = 6) and late luteal endometrium (histology day 23 to 28, n = 6). In addition, alterations in PGF2 alpha synthesis and degradation in response to 17 beta-estradiol (E2) and progesterone (P) were examined to clarify the mechanism of steroid modulation of endometrial PG production (n = 12). Endometrium was maintained in tissue culture and the concentration of PGF2 alpha and 13,14 dehydro-15 keto F2 alpha (DHKF2 alpha) was determined in spent media by radioimmunoassay. Prostaglandin F2 alpha and DHKF2 alpha output from luteal endometrium exposed to P and E2 + P were both significantly reduced when compared with no addition or E2 treatments. This implies that the modulation of PGF2 alpha output by P in vitro is secondary to altered synthesis. There was an increase in PGF2 alpha output from late luteal endometrium when compared with early luteal endometrium in the P and E2 + P treatments, but DHKF2 alpha remained unchanged. These data imply that the temporal increase in PGF2 alpha output is the result of alterations in both PG synthesis and catabolism.
authors: Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington 05405-0068.
authors: J R
authors: JR
authors: Brumsted
authors: J
authors: J
authors: Chapitis
authors: J L
authors: JL
authors: Deaton
authors: D H
authors: DH
authors: Riddick
authors: M
authors: M
authors: Gibson
mesh_terms: D046508:Culture Techniques; D015237:Dinoprost / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D008183:Luteal Phase; D011374:Progesterone / Q000494:pharmacology; D013997:Time Factors
chemical_list: C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost