Revised amino acid sequence of pancreatic spasmolytic polypeptide exhibits greater similarity with an inducible pS2 peptide found in a human breast cancer cell line.
The published amino acid sequence of pancreatic spasmolytic polypeptide (Thim, L., Thomsen, J., Christensen, M. and Jørgensen, K.H. et al. (1985) Biochim. Biophys. Acta 827, 410-418) has been checked by a combination of mass spectroscopy and Edman degradation. The pyroglutamyl blocking group was positively identified, and residue assignments at four positions were corrected: Lys48 (not Ser), Ser63 (not Lys), Cys68 (not Ser) and Ser74 (not Cys). The revised sequence exhibits greater similarity with pS2 peptide, a 60 residue polypeptide which is induced by oestrogen in the human breast cancer cell line MCF-7 and found in malignant but not in non-malignant breast tissue.
Rose
K
K
Département de Biochimie Médicale, C.M.U. Geneva, Switzerland.
Savoy
L A
LA
Thim
L
L
Christensen
M
M
Jørgensen
K H
KH
D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000032:analysis*; D002268:Carboxypeptidases; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010276:Parasympatholytics; D010446:Peptide Fragments; D010455:Peptides; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014357:Trypsin; D014407:Tumor Cells, Cultured
D036341:Intercellular Signaling Peptides and Proteins; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010276:Parasympatholytics; D010446:Peptide Fragments; D010455:Peptides; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; C034407:pancreatic spasmolytic polypeptide; D002268:Carboxypeptidases; D014357:Trypsin