Sequence homology of a canine brain calcium-binding protein with calregulin and the human Ro/SS-A antigen.
A 60 kDa calcium-binding protein (CBP) was purified from canine brain and its N-terminal sequence determined to be: Glu-Pro-Ala-Ile-Tyr-Phe-Lys-Glu-Gln-Phe-Leu-Asp-Gly-Asp-Gly-X-Thr-Arg-X- Ile- Glu-Ser-Lys. This sequence is very similar to that of "Ccalregulin", a CBP of unknown function which is similar in size and appears to be present in most animal tissues. An unexpected and even more striking similarity was found with the N-terminal sequence of the human Ro/SS-A antigen, a 60 kDa protein which has long been implicated in the pathogenesis of autoimmune diseases such as systemic lupus erythematosus. These findings suggest that the Ro/SS-A antigen is probably also a CBP.
Collins
J H
JH
Department of Biological Chemistry, School of Medicine, University of Maryland, Baltimore 21201.
Xi
Z J
ZJ
Alderson-Lang
B H
BH
Treves
S
S
Volpe
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000378:metabolism*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D037282:Calreticulin; D004285:Dogs; D006801:Humans; D008969:Molecular Sequence Data; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D000099207:SS-A Antigen
D001324:Autoantigens; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human