Stimulation of mast cell chemotaxis by interleukin 3.
PWM-activated spleen cell-conditioned medium (SCCM) and a variety of purified hematopoietic growth factors were tested for their ability to stimulate chemotaxis of mouse connective tissue mast cells (CTMC). Of the agents tested, only IL-3 and SCCM promoted mast cell chemotaxis. Neither IL-2, IL-4, GM-CSF, nor endotoxin had any significant mast cell chemotactic activity. Neutralizing antibodies to mouse IL-3 blocked greater than 90% of the chemotactic activity of SCCM, suggesting that IL-3 is the predominant mast cell chemotactic factor produced by activated spleen cells. Our results demonstrate that mature connective tissue type mast cells are capable of moving toward a gradient of spleen cell-derived IL-3 and suggest that movement of mature mast cells toward lymphokines may influence the accumulation of mast cells at sites of inflammatory or immune reactions.
Matsuura
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N
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts.
Zetter
B R
BR
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003239:Connective Tissue Cells; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology*; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D007377:Interleukin-3; D009240:N-Formylmethionine Leucyl-Phenylalanine