Size selected mRNA induces expression of P-aminohippurate transport in Xenopus oocytes.
Xenopus oocytes were injected with size-fractionated mRNA isolated from the renal cortex of rabbit kidney and after 4 days incubation, PAH uptake in oocytes injected with mRNA (0.7-1.3 kb) was 8 to 45 fold that of the water injected controls. The oocyte to medium ratio of accumulated PAH was 1.95. The Km and Vmax for transport were 333 microM and 66.6 nmoles.oocyte-1.min-1, respectively. This Km is similar to that reported for PAH transport in intact kidneys and slices. The uptake of PAH was unaffected by the absence of Na+ or the presence of probenecid. Expression of the transport represents the first step in an effort to clone and identify the gene for PAH transport.
Kwon
O
O
Department of Physiology, S.U.N.Y. Buffalo, New York 14214.
Kwon
H M
HM
Hong
S K
SK
Goldinger
J M
JM
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005796:Genes; D007672:Kidney Cortex / Q000378:metabolism; D007700:Kinetics; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D011817:Rabbits; D014982:Xenopus laevis; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D002352:Carrier Proteins; D012333:RNA, Messenger; D010130:p-Aminohippuric Acid