Cell photosensitization by 5-iminodaunomycin activated with red light.
5-Iminodaunomycin, an anthracycline antitumor drug exhibiting an absorption peak at 595 nm, is shown to photosensitize in vitro cell kill. The photoactivation is performed irradiating the culture dishes during the incubation with the drug for 2 h with 34 mW/cm2 intensity, that is with light doses of up to 245 J/cm2. Long-term effects of administering 50 ng/ml and light for 2 h are studied in terms of growth curves. We show that photoactivation enhances the dark toxicity by a factor of about 10. Immediate cell death is produced by irradiating the cells in the presence of higher drug concentrations (e.g., 1000 ng/ml) which, however, are not toxic in the short term if administered in the dark. The viable cell percentage decreases at increasing light doses, being about 0.6% at the maximum dosage. Administering lower light doses, such as 30 J/cm2, which corresponds to an exposure duration of 15 min, has a short-term effect on the cell survival that strongly depends on the timing of the exposures within the incubation period.
Andreoni
A
A
Centro Endocrinologia ed Oncologia Sperimentale, C.N.R., University of Naples, Italy.
Colasanti
A
A
Malatesta
V
V
Mastrocinque
M
M
Roberti
G
G
von Kisslinger
A
A
D000818:Animals; D000903:Antibiotics, Antineoplastic; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003630:Daunorubicin / Q000031:analogs & derivatives; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques; D008027:Light; D010777:Photochemistry; D011838:Radiation-Sensitizing Agents; D051381:Rats; D013961:Thyroid Gland; D013997:Time Factors
D000903:Antibiotics, Antineoplastic; D011838:Radiation-Sensitizing Agents; C018979:5-iminodaunorubicin; D003630:Daunorubicin