Binding patterns of monoclonal anti-B, anti-H and anti-(Leb + Y) on erythrocytes, imaged in the scanning electron microscope.
Binding patterns of monoclonal IgM anti-B, anti-H and anti-(Leb + Y) antibodies on erythrocytes were visualized in the backscatter electron imaging (BEI) mode of a scanning electron microscope by indirect immunogold labelling. The results were independent of secretor status, supporting the view that type 2 oligosaccharides predominate on erythrocytes. There were considerable cell-to-cell variations of amounts of the antigens, presumably because of differences of antigen development. The amount of H antigens may have been overestimated in previous studies. B and H antigens seemed linked mostly to mobile structures, probably lipid, on the convex parts of the cells and to immobile structures, probably protein, on the concave parts. Y antigens appeared bound to mobile structures also on the concave part of the cell membrane. The results therefore confirm that B oligosaccharides on erythrocytes develop from both lipid- and protein-bound H, but suggest that Y develops mainly from lipid-bound H.
Heier
H E
HE
National Institute of Public Health, Department of Immunology, Oslo, Norway.
Namork
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D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006384:Hemagglutination; D006801:Humans; D007983:Lewis Blood Group Antigens; D008855:Microscopy, Electron, Scanning
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007983:Lewis Blood Group Antigens