Reductive biotransformation of 3-oxo bile acids in human blood.
7 alpha,12 alpha-Dihydroxy-3-oxo-5 beta-cholanoic acid labeled with 18O atoms was incubated with human blood, and the biotransformation products were separated and characterized by gas chromatography-mass spectrometry as the pentafluorobenzyl ester-trimethylsilyl and -dimethylethylsilyl ether derivatives. 3 beta,7 alpha,12 alpha-Trihydroxy-5 beta-cholanoic acid was identified as a main metabolite. When 3-oxo bile acid was incubated with human blood denatured at 70 degrees C for 2 min, no metabolites were formed. The enzymic reduction activity proved to be localized in the red blood cell fraction.
Goto
J
J
Miura
H
H
Ando
M
M
Nambara
T
T
Makino
I
I
D001647:Bile Acids and Salts / Q000378:metabolism*; D001711:Biotransformation; D002793:Cholic Acids / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010084:Oxidation-Reduction
D001647:Bile Acids and Salts; D002793:Cholic Acids; C017234:7,12-dihydroxy-3-oxocholanic acid