CD4 and p56lck can stably associate when co-expressed in NIH3T3 cells.
The CD4 T-cell surface antigen and the lymphocyte-specific tyrosine-protein kinase p56lck form a stable noncovalent complex in CD4+ T-lymphocytes. In this report, we demonstrate that these two gene products can also associate when co-expressed in NIH3T3 fibroblasts, therefore implying that other lymphoid specific components are not required for the CD4-lck interaction. These results also suggest that co-expression of CD4 and p56lck in non-lymphoid cells may prove to be a useful model system for the analysis of structural and possibly functional CD4-lck interactions.
Simpson
S C
SC
Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892.
Bolen
J B
JB
Veillette
A
A
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D005347:Fibroblasts / Q000032:analysis; D051379:Mice; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D014162:Transfection
D000945:Antigens, Differentiation, T-Lymphocyte; D011505:Protein-Tyrosine Kinases