Evidence for unusually short tubulin mRNA leaders and characterization of tubulin genes in Giardia lamblia.
We have cloned alpha- and beta-tubulin cDNAs from Giardia lamblia and have used these to determine the gene copy number in the organism and to isolate alpha- and beta-tubulin genomic clones. Studies of the gene organization demonstrate that two copies of beta-tubulin are linked in a head-to-head configuration. The DNA from these two copies and that from one alpha-tubulin copy has been sequenced upstream of the translation initiation codon, and analyzed for consensus to typical eukaryotic promoter sequences. Characterization of the alpha- and beta-tubulin mRNAs in this parasite by primer extension and S1 nuclease mapping has revealed an unusually short (6 nucleotides) 5' untranslated region.
Kirk-Mason
K E
KE
Department of Biochemical Parasitology, Merck, Sharp, and Dohme Research Laboratories, Rahway, NJ 07065.
Turner
M J
MJ
Chakraborty
P R
PR
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D015342:DNA Probes; D005784:Gene Amplification; D005872:Giardia / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D021382:Protein Sorting Signals / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D015183:Restriction Mapping; D014404:Tubulin / Q000235:genetics*
D015342:DNA Probes; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D014404:Tubulin; D004247:DNA