Vinblastine sensitivity of leukaemic lymphoblasts modulated by serum lipid.
The high-resolution proton magnetic resonance spectrum of leukaemic lymphoblasts is characteristic of neutral lipid in an isotropic environment. When such lymphoblasts are selected for resistance to the anticancer drug vinblastine, the intensity of this spectrum increases with increasing drug resistance. A reversal of this trend can be achieved by growing cells in delipidated serum, whereby lipid spectrum and drug resistance are diminished. However, both can be restored by subsequent regrowth in normal medium. Thus, although detectable genetic changes accompany the development of vinblastine resistance, the expression of these changes can be modulated by environmental lipid.
Holmes
K T
KT
Department of Cancer Medicine, University of Sydney, NSW, Australia.
Dyne
M
M
Williams
P G
PG
May
G L
GL
Tripp
E
E
Wright
L C
LC
Mountford
C E
CE
D002784:Cholesterol / Q000378:metabolism; D003470:Culture Media; D004351:Drug Resistance; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000378:metabolism / Q000473:pathology*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014747:Vinblastine / Q000494:pharmacology*
D003470:Culture Media; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D014747:Vinblastine; D002784:Cholesterol