title: Intraspinal course of descending serotoninergic pathways innervating the rodent dorsal horn and lamina X.
abstract: Although numerous studies have discussed serotoninergic pathways projecting from the brainstem to the spinal cord, the intraspinal course of these descending axons remains a matter of debate. One of the difficulties with traditional immunohistochemical methods is that the interpretation of results has been subjective. We have therefore employed a computer-assisted image-processing system to provide objective measurements of serotonin immuno-reactivity within the rodent dorsal horn and lamina X caudal to unilateral thoracic cordotomy in order to establish the intraspinal course of descending serotoninergic fibers. Lesion analysis combined with computer-determined optical density measurements, high-power microscopic examination, and camera lucida drawings demonstrated that the predominant serotoninergic pathway terminating within the dorsal horn courses unilaterally within the dorsal portion of the dorsolateral funiculus. The thoracic dorsal horn may receive a slightly greater contribution from the contralateral side than the lumbar dorsal horn. Lamina X receives serotoninergic innervation both from fibers descending within the ipsilateral dorsolateral funiculus and from more ventrally located ipsilateral pathways, and it also contains serotonin derived from other sources.
authors: Department of Physiology, University of North Carolina, Chapel Hill 27599.
authors: E
authors: E
authors: Bullitt
authors: A R
authors: AR
authors: Light
mesh_terms: D000818:Animals; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
chemical_list: D012701:Serotonin