title: An immunological study on chromogranin A and B in human endocrine and nervous tissues.
abstract: Antisera were raised against synthetic peptides derived from the primary amino acid sequence of human chromogranin B. These antisera recognized in one- and two-dimensional immunoblotting a component previously designated as chromogranin B. In human chromaffin granules, the major endogenous processing product of chromogranin B is formed by proteolytic cleavage of the protein near the C-terminus. Immunohistochemical localizations were obtained with antisera against human chromogranins A and B and against a synthetic peptide corresponding to the B sequence. In human tissues, chromogranin B is co-stored with chromogranin A in the adrenal medulla, the anterior pituitary, parafollicular cells of the thyroid, in some cells of the endocrine pancreas and in some enterochromaffin cells, whereas only chromogranin A is found in the parathyroid gland and enterochromaffin cells of the gastric corpus mucosa. In the nervous system, no immunostaining was observed for chromogranin A and only a weak one for chromogranin B in some cells of the spinal cord. However, the Purkinje cells of the cerebellum were strongly positive for chromogranin B.
authors: Department of Pathology, University of Innsbruck, Austria.
authors: K W
authors: KW
authors: Schmid
authors: R
authors: R
authors: Weiler
authors: R W
authors: RW
authors: Xu
authors: R
authors: R
authors: Hogue-Angeletti
authors: R
authors: R
authors: Fischer-Colbrie
authors: H
authors: H
authors: Winkler
mesh_terms: D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis*; D004702:Endocrine Glands / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D015151:Immunoblotting; D007150:Immunohistochemistry; D009417:Nerve Tissue / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*
chemical_list: C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D007106:Immune Sera; D009419:Nerve Tissue Proteins