[Synthesis and reactivity of anthracene-1-carbonyl azide as a fluorescent derivatization reagent for alcohols].
Anthracene-1-carbonyl azide was found to be a sensitive fluorescent derivatization reagent for primary and secondary alcohols for high-performance liquid chromatography. Reaction conditions were investigated for cholestanol. The reagent reacts with alcohols in benzene in the presence of 4-dimethylaminopyridine to give the corresponding fluorescent urethanes at 100 degrees C for 50 min. The detection limit for cholestanol was 250 fmol for an injection volume of 10 microliters. Tertiary alcohols and phenols do not produce fluorescent derivatives under these conditions.
Fujino
H
H
Hidaka
M
M
Goya
S
S
D000438:Alcohols / Q000032:analysis*; D000873:Anthracenes / Q000138:chemical synthesis; D001386:Azides / Q000138:chemical synthesis; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D007202:Indicators and Reagents
D000438:Alcohols; D000873:Anthracenes; D001386:Azides; D007202:Indicators and Reagents