The role of marrow accessory cell populations in the augmentation of human erythroid progenitor cell (BFU-E) proliferation by prostaglandin E.
The requirement for CD8+ T lymphocytes in the stimulation of erythroid progenitor cells by prostaglandin E (PGE) was examined. When low density bone marrow (LD-BM) or non-adherent bone marrow (NA-BM) cells were depleted of CD8+ cells the enhancing effect of PGE on BFU-E proliferation was abrogated. However, further enrichment of marrow progenitor cells by depletion of accessory cells using a cocktail of specific monoclonal antibodies, immunoadherence and fluorescence activated cell sorting with the MY10 monoclonal antibody resulted in a population of erythroid progenitor cells which were responsive to the enhancing effect of PGE despite the absence of CD8+ cells. Stepwise individual cell lineage depletion of marrow cell populations indicated that prostaglandin E enhanced erythroid burst formation in the absence of CD8+ cells provided that glycophorin-A+ cells were removed from LD-BM or NA-BM cells. These results suggest that nucleated erythroid cell populations may modulate the enhancement of BFU-E by PGE. The ability of GP-A+ cells to block the enhancement of erythroid burst formation by PGE following removal of CD8+ T cells was confirmed by readdition of conditioned medium prepared from positively selected GP-A+ marrow cells. These results expand the role of CD8+ T cells in the PGE enhancement of BFU-E proliferation and suggest another mechanism by which accessory cells regulate the proliferation of BFU-E in bone marrow.
Nocka
K H
KH
Department of Hematopoietic Regulation, Sloan-Kettering Institute, New York 10021.
Ottman
O G
OG
Pelus
L M
LM
D000938:Antigen-Presenting Cells / Q000502:physiology*; D001853:Bone Marrow / Q000502:physiology*; D002448:Cell Adhesion; D002452:Cell Count; D003470:Culture Media / Q000494:pharmacology; D004900:Erythroblasts / Q000502:physiology*; D004920:Erythropoiesis / Q000187:drug effects*; D006133:Growth Substances / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D008212:Lymphocyte Depletion; D011458:Prostaglandins E / Q000494:pharmacology*; D013601:T-Lymphocytes
D003470:Culture Media; D006133:Growth Substances; D011458:Prostaglandins E