[Comparative studies of measuring condition of activated partial thromboplastin time and contact factors in experimental animals].
For establishing the optimal incubation time (OIT) for measurement of the activated partial thromboplastin time (APTT) in dogs, rabbits, guinea pigs, rats and mice, we determined the shortest clotting time of the plasma from each animal species and compared them with that of human plasma. The OIT for APTT determination was 15 to 30 sec in guinea pigs, rats and mice and 5 to 10 minutes in dogs and rabbits. The mouse APTT (about 30 sec) with the OIT thus determined was similar to human APTT, and relatively longer than APTT in other animal species (10-20 sec). To elucidate the mechanism of the species differences in OIT, we examined the plasma of each animal species for the activity of the contact factors such as factor XII, factor XI, high molecular weight kininogen (HMWK) and prekallikrein (PK) and their effect on the coagulation of contact factor-deficient plasma. The total activity of contact factors was higher in dogs and guinea pigs and lower in rabbits and mice than that in humans. Species difference with the factor XII, Factor XI and HMWK was noted in clotting time but not in OIT. These results suggest that the species difference in OIT for APTT is probably due to difference in activity of the plasma contact factors and in the mode of coagulation for each contact factor.
Noguchi
M
M
Safety Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Fujii
T
T
Minamide
S
S
D000818:Animals; D001780:Blood Coagulation Tests; D004285:Dogs; D005172:Factor XI; D005174:Factor XII; D006168:Guinea Pigs; D006801:Humans; D007704:Kininogens; D051379:Mice; D010314:Partial Thromboplastin Time; D011288:Prekallikrein; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D007704:Kininogens; D005174:Factor XII; D005172:Factor XI; D011288:Prekallikrein