Interposition of different chemical linkages between antibody and 111In-DTPA to accelerate clearance from non-target organs and blood.
Two chemically labile linkages, disulfide and diester, and two stable linkages, thioether and hydrocarbon, were introduced between antibody and 111In-DTPA in order to modify their biodistributions. The biodistributions of the new linkages were evaluated in rats with target antigens localized in lungs. For a comparison purpose, the antibody-DTPA conjugate with a peptide linkage was used as a control conjugate. The antibody conjugates with the stable linkages produced the biodistributions similar to that of the peptide linked conjugate during a 48 h period. The disulfide and diester conjugates, however, cleared from blood much faster and are retained in normal organs much lower than the peptide conjugate. The disulfide and the diester conjugate amplified the lung (target) to blood ratio by 15 and 6 times, respectively at 48 h, as compared to the corresponding target to blood ratio of the control conjugate. Compared to the control conjugate, a 3 times higher target to liver ratio was also obtained by the disulfide conjugate and a 4 times higher target to kidney ratio was obtained by the diester conjugate at 48 h.
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Radiopharmaceutical Chemistry Section, George Washington University Medical Center, Washington, DC 20037.
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D000818:Animals; D000911:Antibodies, Monoclonal; D000922:Immunotoxins / Q000138:chemical synthesis / Q000493:pharmacokinetics; D004369:Pentetic Acid / Q000138:chemical synthesis / Q000493:pharmacokinetics; D051381:Rats; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D004369:Pentetic Acid