The isolation of spermatozoa from sexual assault swabs using proteinase K.
The most common examination in sexual offences is the identification of spermatozoa in aqueous extracts from swabs. Using Proteinase K with sodium dodecyl sulphate, it is possible to digest all the cellular material apart from the spermatozoa, resulting in a quick and effective method of their isolation. Spermatozoa were detected in 18 extracts using this treatment when the normal method had failed to reveal all but one of them.
Chapman
R L
RL
Brown
N M
NM
Keating
S M
SM
D019286:Endopeptidase K; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D012697:Serine Endopeptidases / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate; D013094:Spermatozoa; D014621:Vagina / Q000378:metabolism*
D012967:Sodium Dodecyl Sulfate; D012697:Serine Endopeptidases; D019286:Endopeptidase K