Increased expression of class II antigens of the major histocompatibility complex on alveolar macrophages and alveolar type II cells and interleukin-1 (IL-1) secretion from alveolar macrophages in an animal model of silicosis.
Silicosis is a chronic progressive granulomatous and fibrotic lung disease caused by inhaled silica. Although the causative agent is known, the pathogenesis, especially the immunologic response, is not well understood. We examined two important components of cell-mediated immune responses in the lungs of rats with silica-induced lung disease, i.e., class II (Ia) antigen expression and IL-1 production. The relative density of Ia was examined on isolated alveolar macrophages and type II cells with a solid-phase cellular radioimmunoassay and the percent of Ia positive cells was determined by an indirect immunofluorescent technique. There was a three-fold increase of Ia expression on the alveolar macrophages and nearly a two-fold increase on type II cells from rats with silicosis compared to normal rats. The percent of alveolar type II cells positive for Ia increased by 20%, and the alveolar macrophages increased by 40%. IL-1 in supernatants from cultured alveolar macrophage was measured by the amount of DNA synthesis in an IL-1 dependent cell line (D10). A six-fold increase in IL-1 secretion was noted in macrophage supernatants derived from silica-treated animals. We conclude that in this animal model of silicosis, a local amplification of cell-mediated immune responses may be instrumental in the pathogenesis.
Struhar
D J
DJ
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Harbeck
R J
RJ
Gegen
N
N
Kawada
H
H
Mason
R J
RJ
D000818:Animals; D004195:Disease Models, Animal; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007375:Interleukin-1 / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D011650:Pulmonary Alveoli / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012829:Silicosis / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1