Primary structures of new 'iso-hirudins'.
Crude hirudin (12.7 U/micrograms), a complex mixture of polypeptides obtained from the leech, could be separated by microbore HPLC. A combination of amino acid analysis, N-terminal microsequencing and chemical as well as enzymatic fragmentation made the primary sequence of the new isohirudins Ia-IIIb' accessible. The biological activity determined in the thrombin inhibition test showed a comparable value for all of these compounds. The results presented address the question as to whether these isohirudins are true mutations from a family of genes or a family of leeches.
Scharf
M
M
Institut für organische Chemie, Johann Wolfgang v. Goethe Universität, Frankfurt, FRG.
Engels
J
J
Tripier
D
D
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006629:Hirudins / Q000032:analysis / Q000302:isolation & purification*; D006868:Hydrolysis; D019811:Hydroxylamine; D006898:Hydroxylamines; D007865:Leeches; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D006629:Hirudins; D006898:Hydroxylamines; D010446:Peptide Fragments; D010455:Peptides; D019811:Hydroxylamine; D014357:Trypsin