title: Reductive biotransformation of 3-oxo bile acids in human blood.
abstract: 7 alpha,12 alpha-Dihydroxy-3-oxo-5 beta-cholanoic acid labeled with 18O atoms was incubated with human blood, and the biotransformation products were separated and characterized by gas chromatography-mass spectrometry as the pentafluorobenzyl ester-trimethylsilyl and -dimethylethylsilyl ether derivatives. 3 beta,7 alpha,12 alpha-Trihydroxy-5 beta-cholanoic acid was identified as a main metabolite. When 3-oxo bile acid was incubated with human blood denatured at 70 degrees C for 2 min, no metabolites were formed. The enzymic reduction activity proved to be localized in the red blood cell fraction.
authors: J
authors: J
authors: Goto
authors: H
authors: H
authors: Miura
authors: M
authors: M
authors: Ando
authors: T
authors: T
authors: Nambara
authors: I
authors: I
authors: Makino
mesh_terms: D001647:Bile Acids and Salts / Q000378:metabolism*; D001711:Biotransformation; D002793:Cholic Acids / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010084:Oxidation-Reduction
chemical_list: D001647:Bile Acids and Salts; D002793:Cholic Acids; C017234:7,12-dihydroxy-3-oxocholanic acid