Investigations into the membrane associated IL-1 like activity on mouse macrophages.
The present study was undertaken to investigate the expression of membrane IL-1 (mIL-1) in mouse macrophages. Membrane IL-1 activity was measured on paraformaldehyde fixed peritoneal macrophages using EL4 6.1 lymphocyte cell line. Acid washing prior to fixation did not remove the mIL-1 activity, indicating that mIL-1 is an integral part of the membrane. Adherence to tissue culture plastic plates was found to be a potent inducer of mIL-1 activity in both resident and C. parvum activated macrophages. Dexamethasone reduced mIL-1 activity induced in vivo by C. parvum and also that induced by adherence in vitro.
Kidgell
A E
AE
Department of Cellular Sciences, Glaxo Group Research Ltd. Greenford Middlesex, UK.
Paska
W
W
Humphray
H P
HP
Mackenzie
A R
AR
D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D007375:Interleukin-1 / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice
D007375:Interleukin-1; D003907:Dexamethasone