Studies on the energy metabolism of opossum Didelphis virginiana erythrocytes--IV. Red cells have low adenosine deaminase activity and high levels of deoxyadenosine nucleotides.
Alkaline extracts of adult opossum red cells were used to determine triphosphates of adenosine, deoxyadenosine and guanosine by anion exchange HPLC. Mean (nm/g Hg) ATP content of erythrocytes was 3713 and that of dATP 1913 (n = 12). Sonicates of red cells deaminated adenosine (ADO) at a rate of 1.55 nm/mg Hg/h and deoxyadenosine (dADO) at 1.82 nm/mg Hg/h. dATP synthesis from provided dADO was one order of magnitude greater in opossum than in human erythrocytes at both low and high dADO and Pi concentrations.
Bethlenfalvay
N C
NC
Department of Primary Care and Clinical Investigation, Fitzsimons Army Medical Center, Aurora, Colorado 80045.
Chadwick
E
E
Lima
J E
JE
D000241:Adenosine / Q000097:blood; D000243:Adenosine Deaminase / Q000097:blood*; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003838:Deoxyadenine Nucleotides / Q000097:blood*; D003839:Deoxyadenosines / Q000097:blood; D004734:Energy Metabolism; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000378:metabolism*; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009700:Nucleoside Deaminases / Q000097:blood*; D009893:Opossums / Q000097:blood*
D003838:Deoxyadenine Nucleotides; D003839:Deoxyadenosines; D006454:Hemoglobins; D000255:Adenosine Triphosphate; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D000241:Adenosine; C026600:2'-deoxyadenosine triphosphate