In vivo visualization of oxyradical-dependent photoemission during endothelium-granulocyte interaction in microvascular beds treated with platelet-activating factor.
Oxyradical-dependent chemiluminescence from granulocytes sticking to venular endothelium was successfully visualized in rat mesenteric microvascular beds treated with platelet-activating factor (PAF). Intravital photonic image intensifier microscopy revealed that topical application of PAF-acether (100 nM) caused remarkable granulocyte adherence on endothelial walls and the subsequent activation of a luminol-dependent photonic burst. Chemilumigenic sites clearly corresponded to the spatial distribution of sticking cells in post-capillary venules. The present findings thus serve as the first demonstration of an intravital oxidative burst of granulocytes on venular endothelium in PAF-induced microcirculatory disturbances.
Suematsu
M
M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo.
Kurose
I
I
Asako
H
H
Miura
S
S
Tsuchiya
M
M
D000818:Animals; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005609:Free Radicals; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D010101:Oxygen Consumption; D010972:Platelet Activating Factor / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013152:Splanchnic Circulation / Q000187:drug effects; D013997:Time Factors
D005609:Free Radicals; D010972:Platelet Activating Factor; D008165:Luminol