Selective expression of class II E alpha d gene in transgenic mice.
Class II genes of the MHC must be expressed by APC for activation of CD4+ T cells and efficient delivery of T cell help to B lymphocytes. Class II genes have restricted tissue expression and are under complex regulation. By using various deletion constructs of the class II E alpha d gene in transgenic mice we have mapped different 5' flanking regions which control E alpha d gene expression in distinct cell types. We demonstrate dissociate expression of E alpha d within the macrophage lineage as well as within the B cell lineage, and present evidence for a repressive element operative in B cells and macrophages. We describe the generation of novel transgenic lines with limited constitutive and inducible E alpha mRNA and I-E protein.
Burkly
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LC
Biogen Research Corporation, Cambridge, MA 02142.
Lo
D
D
Cowing
C
C
Palmiter
R D
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Brinster
R L
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Flavell
R A
RA
D000818:Animals; D001202:Ascitic Fluid / Q000032:analysis; D001402:B-Lymphocytes / Q000032:analysis; D001482:Base Composition; D001483:Base Sequence; D002872:Chromosome Deletion; D005260:Female; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000235:genetics*; D008264:Macrophages / Q000032:analysis; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D013154:Spleen; D013950:Thymus Gland
D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen