Metabolism of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) by bovine brain microvessel endothelial cells.
Incubation of bovine brain microvessel endothelial cell monolayers with the dopaminergic neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine led to the monoamine oxidase (MAO)-mediated formation of the oxidative metabolites 1-methyl-4-phenyl-2,3-dihydropyridine and 1-methyl-4-phenylpyridine (MPP+). The flux of low nanomolar concentrations of [3H]MPP+ across endothelial cell monolayers appeared to be restricted, probably due to the oxidation by MAO. [3H]MPP+ did not significantly cross endothelial cell monolayers.
Scriba
G K
GK
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas, Lawrence 66045.
Borchardt
R T
RT
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000493:pharmacokinetics*; D015655:1-Methyl-4-phenylpyridinium / Q000493:pharmacokinetics*; D000818:Animals; D001921:Brain / Q000098:blood supply*; D002199:Capillary Permeability; D002417:Cattle; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015655:1-Methyl-4-phenylpyridinium