title: Morphological and functional responses of rat Leydig cells to a prolonged treatment with human chorionic gonadotropins.
abstract: The morphological and functional responses of rat Leydig cells to a 3- and 6-day treatment with human chorionic gonadotropins (hCG) (10 IU/kg/day) were investigated by morphometric and radioimmunological techniques. hCG-administration induced a notable time-dependent enhancement in the steroidogenic capacity and growth of Leydig cells; this last was almost exclusively due to hypertrophy (and not to hyperplasia). The volume of mitochondrial and peroxisome compartments, as well as the surface area per cell of mitochondrial cristae and smooth endoplasmic reticulum (SER) were significantly increased after hCG treatment, and showed a highly significant positive linear correlation with both basal and stimulated testosterone production by isolated Leydig cells of the contralateral testis. Also the volume of nuclei and lipid-droplet compartment and the surface area per cell of Golgi apparatus displayed a notable hCG-induced rise, but they did not correlate with testosterone secretion. These findings suggest that, in addition to mitochondria and SER, in which the enzymes of steroid synthesis are located, peroxisomes are also specifically involved in the secretory activity of rat Leydig cells.
authors: Department of Anatomy, University of Padua, Italy.
authors: P G
authors: PG
authors: Andreis
authors: L
authors: L
authors: Cavallini
authors: L K
authors: LK
authors: Malendowicz
authors: A S
authors: AS
authors: Belloni
authors: P
authors: P
authors: Rebuffat
authors: G
authors: G
authors: Mazzocchi
authors: G G
authors: GG
authors: Nussdorfer
mesh_terms: D000818:Animals; D002452:Cell Count / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000096:biosynthesis
chemical_list: D006063:Chorionic Gonadotropin; D013739:Testosterone