Enhanced malignant phenotype of urethane-induced lung adenoma associated with sialoadenectomy in BALB/c mice.
Mouse lung adenoma were induced in adult female BALB/c mice by chronic i.p. urethane injection. Sialoadenectomy of the mice prior to carcinogen treatment did not alter the frequency or size of lung adenoma. However, sialoadenectomized mice exhibited a decrease in the proportion (18 versus 52%) of tumours which exhibited a low nuclear to cytoplasmic ratio and which grew along alveolar septa. Sialoadenectomy was also related to a complementary increase in the proportion (82 versus 48%) of tumours with large vesicular nuclei, prominent nucleoli and generally increased phenotypic features of malignancy. Replacement of epidermal growth factor (EGF) in sialoadenectomized mice restored the tumour-type ratio observed in non-sialoadenectomized mice. These data are discussed with respect to the possible roles of EGF in mouse lung alveologenic carcinoma formation and the cell type of origin for the tumours observed.
Smith
G J
GJ
Carcinogenesis Research Unit, School of Pathology, University of New South Wales, Kensington, Australia.
Rhodes
G C
GC
Moore
G P
GP
D000236:Adenoma / Q000139:chemically induced* / Q000473:pathology; D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D013363:Submandibular Gland / Q000502:physiology*; D014520:Urethane
D014520:Urethane; D004815:Epidermal Growth Factor