Isolation of interleukin 1 from joint fluids of patients with Lyme disease.
To investigate the role of interleukin 1 (IL-1) in Lyme arthritis we assayed synovial fluids (SF) for the presence of IL-1 activity. Crude SF from patients with Lyme disease showed IL-1-like activity. Chromatography of joint fluids revealed activity at 15-20,000 daltons. Two populations of cells were grown, which produced significant IL-1 activity when stimulated with the Lyme disease spirochete or its lipopolysaccharide. IL-1 activity from SF or stimulated cells was neutralized with an antihuman IL-1 antibody. Our results suggest IL-1 is important in the pathogenesis of Lyme arthritis, and is similar to other arthritides.
Beck
G
G
Department of Pathology, SUNY, Stony Brook 11794.
Benach
J L
JL
Habicht
G S
GS
D001898:Borrelia / Q000378:metabolism / Q000472:pathogenicity; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D006801:Humans; D007375:Interleukin-1 / Q000302:isolation & purification* / Q000494:pharmacology; D008193:Lyme Disease / Q000209:etiology / Q000276:immunology*; D013582:Synovial Fluid / Q000032:analysis*
D007375:Interleukin-1