Structures of two genes coding for melanin-concentrating hormone of chum salmon.
Two MCH genes coding for melanin-concentrating hormone (MCH) were isolated from a chum salmon liver DNA library and characterized. They were shown to be intronless genes with 0.63-kb exons, each of which commonly consisted of an about 80-bp 5'-untranslated region, a region coding for 132 amino acids (aa) MCH precursor protein and an approx. 160-bp 3'-untranslated region. About 20 bp upstream from the putative cap site, sequences were found corresponding to the TATA box. The two genes were 86% identical at the nucleotide sequence level. Sequences homologous to the chum salmon MCH genes were present in the genomes of other fish such as catfish, carp and Chinese grass carp, whereas no highly homologous sequence could be detected in other vertebrate genomes.
Takayama
Y
Y
Department of Molecular Biology, School of Medicine, Kitasato University, Kanagawa, Japan.
Wada
C
C
Kawauchi
H
H
Ono
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005260:Female; D007028:Hypothalamic Hormones; D008543:Melanins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010907:Pituitary Hormones; D011498:Protein Precursors / Q000235:genetics; D012315:RNA Caps; D015183:Restriction Mapping; D012474:Salmon / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014714:Vertebrates / Q000235:genetics
D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D011498:Protein Precursors; D012315:RNA Caps; C019463:melanin-concentrating hormone; D004247:DNA