Copper-thionein in melanoma.
The phenomenon of an elevated copper concentration in melanoma tumors was examined. It was demonstrated that 50-60% of total tissue copper is associated with metallothionein. The amino acid composition, electronic absorption and fluorescence were identical to that of the many known vertebrate Cu-thioneins. The immunological identification of melanoma tissue metallothionein was successful. The elevated Cu-thionein concentration in melanoma tumor tissue is not yet understood. It appears to be a common concept that in most tumors transient changes of the copper status parallel the metallothionein levels.
Krauter
B
B
Anorganische Biochemie, Physiologisch-Chemisches Institut der Universität Tübingen, F.R.G.
Nagel
W
W
Hartmann
H J
HJ
Weser
U
U
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D003300:Copper / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D006801:Humans; D015151:Immunoblotting; D008099:Liver / Q000032:analysis; D008545:Melanoma / Q000032:analysis* / Q000662:veterinary; D008623:Mercaptoethanol / Q000494:pharmacology; D008668:Metallothionein / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D013053:Spectrophotometry
D000596:Amino Acids; C007121:copper thionein; D008623:Mercaptoethanol; D003300:Copper; D008668:Metallothionein