[Experimental studies on influences of intermittent hemihepatic vascular occlusion].
The influences of intermittent hemihepatic vascular occlusion in rats were investigated. The restoration of tissue energy level during 10 min of reperfusion after more than 40 min of occlusion was significantly lower than after occlusion within 30 min. over 40 min of occlusion which resulted in a significant increase of tissue free fatty acids during occlusion showed marked decrease of total adenine nucleotides (TAN) in the liver and marked increase of serum TAN in the hepatic vein after reperfusion. It was presumed that these findings were caused by wash-out of adenine-nucleotides from liver cell to the hepatic vein after reperfusion correlated with degradation of cell membrane. Thus, less than 30 min of occlusion was safe in hemihepatic vascular occlusion. Three times intermittent occlusion--repeats of 30 min of occlusion and 10 min of reperfusion--resulted in a same tissue energy level as once 40 min of occlusion. Furthermore, there was no difference in serum ornithine carbamoyltransferase, tissue oxygen consumption and ICG test during 48 hrs after reperfusion between three times intermittent occlusion and once 40 min of occlusion. These findings indicated that repetition of short time occlusion was useful in order to safely prolong total ischemic time in hemihepatic vascular occlusion.
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First Department of Surgery, Yamagata University School of Medicine, Japan.
D000227:Adenine Nucleotides / Q000097:blood / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006503:Hepatic Veins; D007511:Ischemia / Q000097:blood / Q000378:metabolism*; D008026:Ligation; D008099:Liver / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009954:Ornithine Carbamoyltransferase / Q000097:blood; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion; D013997:Time Factors
D000227:Adenine Nucleotides; D005230:Fatty Acids, Nonesterified; D009954:Ornithine Carbamoyltransferase