Differential effects of liposome-entrapped desferrioxamine on proliferation and erythroid differentiation of murine erythroleukemic Friend cells.
It is known that iron chelators (such as desferrioxamine) are potent inhibitors of both cell proliferation and erythroid differentiation. We have shown with in vitro studies that in the case of tumor cells desferrioxamine is even more efficient in inhibiting cell proliferation when entrapped in liposomes consisting of egg yolk phosphatidylcholine. At the same time liposome-entrapped desferrioxamine retains only a slight effect on hexamethylenebisacetamide induction of erythroid differentiation and hemoglobin accumulation of murine erythroleukemic Friend cells. Based on these findings, we propose liposome-entrapped desferrioxamine as potential antineoplastic agent as well as a specific chemical for the study of both iron metabolism and distribution in normal and neoplastic cells. In addition, unlike free desferrioxamine, the liposome-entrapped drug could also be used in combination with inducers of differentiation. With respect to this issue, it is possible that liposome-entrapped desferrioxamine, might permit erythroid differentiation of both neoplastic cells as well as normal stem cells.
Nastruzzi
C
C
Institut für Polymere, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Walde
P
P
Menegatti
E
E
Gambari
R
R
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003676:Deferoxamine / Q000494:pharmacology*; D005614:Freeze Fracturing; D005622:Friend murine leukemia virus; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D008081:Liposomes; D051379:Mice; D008854:Microscopy, Electron; D010713:Phosphatidylcholines
D008081:Liposomes; D010713:Phosphatidylcholines; D003676:Deferoxamine