A simple, rapid method for screening hybridomas producing monoclonal antibodies to platelet proteins.
Several parameters influence the outcome of somatic cell fusions based on the Köhler and Milstein technology, and a number of steps are of critical importance, including the screening strategy. The procedure chosen, appropriate for the type of antibody required, should be rapid and sensitive, in order to clone the relevant hybrids as quickly as possible. A simple and quick dot blot-based method is reported, suitable for screening hybridoma culture supernatants in order to identify clones producing monoclonal antibodies to platelet constituents.
Perutelli
P
P
Department of Pediatric Hematology and Oncology, G. Gaslini Research Children's Hospital, Genoa, Italy.
Marchese
P
P
Mori
P G
PG
Damiani
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D003101:Collodion; D003470:Culture Media; D004306:Dose-Response Relationship, Immunologic; D006825:Hybridomas / Q000276:immunology*; D015151:Immunoblotting / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D010980:Platelet Membrane Glycoproteins; D003101:Collodion