Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor.
Three major autophosphorylation sites are located near the C-terminus of the epidermal growth factor receptor, but a fourth site is repeatedly detected. We report here the purification and sequencing of a tryptic peptide containing this site, Tyr-1086. Furthermore, we demonstrate that additional phosphopeptides are observed following both partial digestion and overdigestion. Finally, we show that Tyr-1086 can be phosphorylated in intact cells.
Hsuan
J J
JJ
Structural Biology Group, Ludwig Institute for Cancer Research, London, U.K.
Totty
N
N
Waterfield
M D
MD
D000595:Amino Acid Sequence; D066246:ErbB Receptors / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010766:Phosphorylation
D066246:ErbB Receptors