Effect of age on the abundance and fragmentation of link protein of the human intervertebral disc.
The link proteins of the human intervertebral disc were studied in tissue extracts by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE), followed by immunoblotting, using a specific monoclonal antibody. Three link proteins were detected, corresponding in electrophoretic mobility to those present in articular cartilage. As with articular cartilage, the largest link protein predominates in the young, whereas in the adult the smallest link protein is equally abundant and internal fragmentation of the link proteins occurs. Only in the newborn is the quantity of extractable link protein comparable to that from articular cartilage. In the adult, the disc contains much less link protein than is present in autologous articular cartilage. Neither the amount nor heterogeneity of the link protein differs among different levels within the lumbar spine, although the proportions of the three proteins can differ between the anulus fibrosus and nucleus pulposus. The anulus always contained more extractable link protein relative to tissue wet weight than the nucleus, and the nuclear link protein, at least in adolescents, contained a greater proportion of the smallest link protein. Such changes in the quantity and structure of the disc link proteins may affect the properties of the proteoglycan aggregates and, thus, could influence disc function.
Pearce
R H
RH
Department of Pathology, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Mathieson
J M
JM
Mort
J S
JS
Roughley
P J
PJ
D000375:Aging; D000911:Antibodies, Monoclonal / Q000276:immunology; D016326:Extracellular Matrix Proteins; D006801:Humans; D015151:Immunoblotting; D007403:Intervertebral Disc / Q000032:analysis*; D011506:Proteins / Q000378:metabolism*; D011509:Proteoglycans; D014020:Tissue Extracts; D000099249:Proteoglycan Link Protein
D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D011506:Proteins; D011509:Proteoglycans; D014020:Tissue Extracts; D000099249:Proteoglycan Link Protein