title: Isolation and purification by high performance liquid chromatography of a tetanus toxin fragment (fragment [A-B]) derived from mildly papain-treated toxin.
abstract: Fragment [A-B] of tetanus toxin was highly purified by combination of gel permeation chromatography, adsorption chromatography in an HPLC and immunoadsorption chromatography using anti-Fragment [C] as a ligand. The purified Fragment [A-B] (200 micrograms) elicited a peculiar toxicity, 'hypoactivity' or 'weakness', and killed the mice in ca. 73 hr and 88 hr when it was injected i.v. and i.m., respectively. However, contamination by the whole toxin was not detectable, in the purified fragment preparation, when up to 600 micrograms was tested by the mouse toxicity assay.
authors: Department of Tuberculosis Research I, Osaka University, Japan.
authors: K
authors: K
authors: Ozutsumi
authors: D L
authors: DL
authors: Lei
authors: N
authors: N
authors: Sugimoto
authors: M
authors: M
authors: Matsuda
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D051379:Mice; D010206:Papain; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D013744:Tetanus Toxin / Q000032:analysis* / Q000633:toxicity
chemical_list: D010446:Peptide Fragments; D013744:Tetanus Toxin; D010206:Papain