Net increase of pluripotential hematopoietic precursors in suspension culture in response to IL-1 and IL-3.
A new short-term suspension culture system is described in which pluripotential hematopoietic precursors from mouse bone marrow increase 8 to 12 times in number over a 4-day period. The increase is shown to derive from myeloid precursors undergoing repeated cell divisions prior to definitive lineage restriction. The response, which occurs in the absence of any pre-formed feeder layer, depends on dual stimulation by both IL-1 and IL-3, and the maximum effect depends on the presence of both factors together from the initiation of the cultures. The observations extend the known range of targets of IL-1 and IL-3 to include primitive pluripotential precursors capable of some degree of self-renewal, and provide a specific and relatively simple assay both for the precursors and the soluble factors which regulate them.
Iscove
N N
NN
Ontario Cancer Institute, Toronto, Canada.
Shaw
A R
AR
Keller
G
G
D000818:Animals; D001853:Bone Marrow; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004247:DNA / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D007375:Interleukin-1 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D007700:Kinetics; D007958:Leukocyte Count; D051379:Mice; D008808:Mice, Inbred CBA; D013535:Suspensions
D007375:Interleukin-1; D007377:Interleukin-3; D013535:Suspensions; D004247:DNA