Rearrangement by inversion of a T-cell receptor delta variable region gene located 3' of the delta constant region gene.
We have located a T-cell receptor variable (V) delta gene segment immediately 3' of the delta constant (C) region gene and 5' to the known joining (J) alpha gene segments. This V delta gene is in the opposite transcriptional polarity to C delta and has rearranged to C delta by inversion in a gamma/delta-expressing hybridoma, DN7.3. This V delta gene is commonly rearranged in adult but not fetal gamma/delta-expressing thymocytes and has not been observed among alpha gene rearrangements reported to date. The reciprocal joining sequence isolated from this cell line contains N region nucleotides between the recombination signal sequences, in contrast to previously analyzed reciprocal joints. The results are discussed in the context of models accounting for ordered V gene usage during lymphocyte development.
Korman
A J
AJ
Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142.
Maruyama
J
J
Raulet
D H
DH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D007446:Chromosome Inversion; D003001:Cloning, Molecular; D015329:Gene Rearrangement, T-Lymphocyte; D005796:Genes; D014644:Genetic Variation; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell