Synthesis of [Glu34]human splenin (hSP) and examination of its immunological effect on the reduced B-lymphocytes of uremic patients.
[Glu34]human splenin (hSP) was synthesized in a conventional manner by assembling ten peptide fragments followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole (molar ratio, 1:1) in trifluoroacetic acid in the presence of m-cresol and dimethylselenide. Finally, the deprotected peptide was incubated with dithiothreitol to reduce sulfoxide on the methionine side chain. Incubation of peripheral lymphocytes isolated from uremic patients with the synthetic [Glu34]hSP showed an enhancing effect on the reduced B-lymphocytes, but synthetic human thymopoietin (hTP) had no effect under the same conditions.
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D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D013946:Thymopoietins / Q000138:chemical synthesis* / Q000276:immunology / Q000494:pharmacology; D013951:Thymus Hormones / Q000138:chemical synthesis*; D014511:Uremia / Q000097:blood*
D000596:Amino Acids; D013946:Thymopoietins; D013951:Thymus Hormones; C032841:thymopoietin III