Interleukin 1 induction of a serine esterase in a murine T cell line is inhibited by fetal calf serum.
In PC60 cells a serine esterase associated with the cytotoxic granules (BLT-esterase) is induced by interleukin 1 (IL-1). The induction was markedly reduced by fetal calf serum (FCS). A defined culture medium was therefore developed in which the PC60 cells proliferated equally well as in serum-containing medium. In this new medium, IL-1 induced BLT-esterase to much higher levels of activity than in serum-containing medium. When testing IL-1 induction of BLT-esterase with various batches of FCS, no correlation was found between cell proliferation and the responses to IL-1. It was concluded that FCS contains one (or several) inhibitor(s) of IL-1 action.
Schlegel-Haueter
S E
SE
Department of Biochemistry, University of Geneva, Switzerland.
Aebischer
F
F
D000818:Animals; D002417:Cattle / Q000097:blood; D002460:Cell Line; D003470:Culture Media / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005312:Fetal Blood / Q000502:physiology; D053804:Granzymes; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D003470:Culture Media; D007375:Interleukin-1; D011994:Recombinant Proteins; D053804:Granzymes; D012697:Serine Endopeptidases