title: Thioredoxin and glutaredoxin systems of rat liver cytosol are not influenced by thyroid dysfunction.
abstract: The relation of thyroid hormone status to the function of hepatic cytosolic components activating microsomal reverse triiodothyronine (rT3) 5'-monodeiodination was studied in rats. Hyperthyroidism was induced by administration of thyroxine and hypothyroidism, by thyroidectomy. The DTT-stimulated microsomal rT3 5'-monodeiodination rate was increased by 125% in hyperthyroid rats and reduced to about 30% in hypothyroid rats (when compared with euthyroid animals). Thyroid status was unrelated to NADPH-dependent activation of microsomal 5'-deiodinase by cytosol components or to cytosolic concentrations of thioredoxin and glutaredoxin, which stimulate in vitro microsomal deiodination of thyroid hormone.
authors: Thyroid Research Laboratory and Endocrine Division, Mount Sinai Hospital, Toronto, Ont., Canada.
authors: G B
authors: GB
authors: Bhat
authors: B C
authors: BC
authors: Hummel
authors: P G
authors: PG
authors: Walfish
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism*; D054477:Glutaredoxins; D005978:Glutathione / Q000378:metabolism; D007453:Iodide Peroxidase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009249:NADP / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010088:Oxidoreductases; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology; D013879:Thioredoxins / Q000378:metabolism*; D013959:Thyroid Diseases / Q000378:metabolism*; D013963:Thyroid Hormones / Q000097:blood; D013965:Thyroidectomy
chemical_list: D001426:Bacterial Proteins; D054477:Glutaredoxins; D011506:Proteins; D013963:Thyroid Hormones; D013879:Thioredoxins; D009249:NADP; D010088:Oxidoreductases; D007453:Iodide Peroxidase; D005978:Glutathione