Identification of serum-responsive elements in the promoter of human calcyclin, a growth-regulated gene.
The calcyclin gene is growth regulated in a number of cell types from different animal species. We have identified in the promoter region of the human calcyclin gene the sequences responsible for its growth regulation by serum. A number of deletion mutants and synthetic nucleotides, all driving the chloroamphenicol transferase (CAT) gene, were constructed for this purpose. Serum-responsive elements can be localized in the last 42 bp immediately upstream from the CAP site.
Ghezzo
F
F
Department of Pathology, Temple University Medical School, Philadelphia, PA 19140.
Valpreda
S
S
De Riel
J K
JK
Baserga
R
R
D000818:Animals; D001483:Base Sequence; D001769:Blood; D002135:Calcium-Binding Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D006224:Cricetinae; D004815:Epidermal Growth Factor / Q000235:genetics*; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D010957:Plasmids; D011401:Promoter Regions, Genetic; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins
D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D006133:Growth Substances; D009838:Oligodeoxyribonucleotides; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489134:S100a6 protein, mouse; C055351:S100A6 protein, human; D004815:Epidermal Growth Factor; D015500:Chloramphenicol O-Acetyltransferase