title: Induction of the insulin receptor and other differentiation markers by sodium butyrate in the Burkitt lymphoma cell, Raji.
abstract: The very low expression of insulin receptors in the Burkitt lymphoma cell Raji was increased 2-fold, 6-fold and 10-fold after 1, 2 and 3 days, respectively, by incubation with the differentiation inducer sodium butyrate. Insulin receptor number was increased without a change in receptor affinity, in association with an increase in the receptor alpha and beta subunits detected after cell-surface labelling and immunoprecipitation. Expression of cell-surface class I and II human leukocyte antigens, the intercellular adhesion molecule-1 and the CD38 leukocyte antigen was also increased, consistent with B cell differentiation. Butyrate effects were not unspecific, as the binding of tumour necrosis factor and growth hormone and the expression of the B cell markers CD20, B5 and CD21 was not increased. The low expression of insulin receptors on Raji cells is therefore a reflection of the less differentiated state of these cells compared to lymphoblastoid cells.
authors: Burnet Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
authors: J D
authors: JD
authors: Newman
authors: G S
authors: GS
authors: Eckardt
authors: A
authors: A
authors: Boyd
authors: L C
authors: LC
authors: Harrison
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis / Q000096:biosynthesis*; D001667:Binding, Competitive; D002051:Burkitt Lymphoma / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D011972:Receptor, Insulin / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D011972:Receptor, Insulin