Polyclonal origin of mouse skin papillomas.
We show that, from the earliest morphologically recognisable stages of development, mouse skin papillomas induced by a chemical initiation-promotion regime are polyclonal. We have demonstrated polyclonality directly by immunohistochemical staining of the mosaic cell populations in embryo aggregation chimaeras, a method which removes some of the uncertainties of previous conclusions based on analysis using electrophoretic polymorphisms. The findings may imply that initiation within a single cell and promotion of its clonal descendents is not a sufficient explanation for the origin of these tumours and that interaction between cells of more than one clone is involved.
Winton
D J
DJ
Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK.
Blount
M A
MA
Ponder
B A
BA
D000818:Animals; D002678:Chimera; D004817:Epidermis / Q000473:pathology; D006965:Hyperplasia / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010212:Papilloma / Q000473:pathology*; D011230:Precancerous Conditions / Q000473:pathology*; D012878:Skin Neoplasms / Q000473:pathology*