title: An NADH:Fe(III)EDTA oxidoreductase from Cryptococcus albidus: an enzyme involved in ethylene production in vivo?
abstract: An ethylene-forming enzyme which forms ethylene from 2-oxo-4-methylthiobutyric acid (KMBA) was purified to an electrophoretically homogeneous state from a cell-free extract of Cryptococcus albidus IFP 0939. The presence of KMBA, NADH, Fe(III) chelated to EDTA and oxygen were essential for the formation of ethylene. When ferric ions, as Fe(III)EDTA, in the reaction mixture were replaced by Fe(II)EDTA under aerobic conditions, the non-enzymatic formation of ethylene was observed. Under anaerobic conditions in the presence of Fe(III)EDTA and NADH, the enzyme reduced 2 mol of Fe(III) with 1 mol of NADH to give 2 mol of Fe(II) and 1 mol NAD+, indicating that the ethylene-forming enzyme is an NADH-Fe(III)EDTA oxidoreductase. The role of NADH:Fe(III)EDTA oxidoreductase activity in the formation in vivo ethylene from KMBA is discussed.
authors: Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan.
authors: H
authors: H
authors: Fukuda
authors: M
authors: M
authors: Takahashi
authors: T
authors: T
authors: Fujii
authors: M
authors: M
authors: Tazaki
authors: T
authors: T
authors: Ogawa
mesh_terms: D002087:Butyrates / Q000378:metabolism; D003454:Cryptococcus / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005030:Ethylenes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds
chemical_list: D002087:Butyrates; D005030:Ethylenes; D013438:Sulfhydryl Compounds; C045636:2-keto-4-mercaptobutyric acid; C036216:ethylene; D009247:NADH, NADPH Oxidoreductases; C061239:NADH-Fe(III)EDTA oxidoreductase