4-beta-phorbol-12,13-dibutyrate enhances K+-stimulated dopamine release from hippocampal slices.
Slices of rat hippocampus were labelled with [14C]dopamine, superfused continuously with oxygenated Krebs-Henseleit solution and stimulated with a potassium pulse (48 mM K+, 5 min.). 4-beta-phorbol-12,13-dibutyrate (PDB), an activator of protein kinase C (PKC), enhanced the potassium-evoked overflow of 14C. This effect was blocked by prior application of polymyxin B, a relatively selective inhibitor of PKC. In contrast, the PKC-inactive 4-alpha-phorbol-12,13-didecanoate (PDD) had no influence on the evoked transmitter overflow. The results suggest that PKC may also be involved in the regulation of hippocampal DA release. A possible link between PKC activation and DA release to processes of synaptic long-term potentiation is discussed.
Schröder
H
H
Institute of Pharmacology and Toxicology, Medical Academy Magdeburg, GDR.
Reymann
K G
KG
Matthies
H
H
D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D004357:Drug Synergism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D011112:Polymyxin B / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D009419:Nerve Tissue Proteins; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D011112:Polymyxin B; D011188:Potassium; D004298:Dopamine