Detection and characterization of a B cell stimulatory factor (BSF-TC) derived from a bone marrow stromal cell line.
Stromal cell lines derived from murine bone marrow support the growth of immature pre-B cells and produce cytokines that affect the growth and differentiation of other hematopoietic precursors. Conditioned medium (CM) from one such line (TC-1) stimulated marked proliferation of B cells previously activated by anti-Ig (anti-Ig blasts). Proliferation of anti-Ig blasts was not induced by purified cytokines known to be produced by TC-1 (CSF-1, GM-CSF, or G-CSF) or by IL-1, IL-2, IL-3, IL-4, IL-5, or IL-6. Furthermore, IL-2, IL-4, and IL-5, alone or in combination, failed to support proliferation or differentiation of anti-Ig blasts. TC-1 CM enhanced proliferation of B cells that were co-cultured with LPS, anti-Ig, or dextran sulfate; co-stimulation with anti-Ig was unaffected by the presence of monoclonal anti-IL-4. Proliferation of low, but not high, density B cells isolated from spleen was directly stimulated by TC-1 CM. These results suggest that bone marrow stromal cells produce a novel B cell stimulatory factor (BSF-TC) that induces proliferation of activated B cells.
Simpson
L
L
Department of Pharmacology, University of Virginia School of Medicine, Charlottesville 22908.
McNiece
I
I
Newberg
M
M
Schetz
J
J
Lynch
K R
KR
Quesenberry
P
P
Isakson
P C
PC
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology* / Q000502:physiology; D001853:Bone Marrow / Q000032:analysis* / Q000502:physiology; D002460:Cell Line; D003470:Culture Media; D004357:Drug Synergism; D005109:Extracellular Matrix / Q000032:analysis* / Q000502:physiology; D006133:Growth Substances / Q000502:physiology; D015847:Interleukin-4; D007378:Interleukins / Q000032:analysis* / Q000502:physiology; D007399:Interphase / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C
D000888:Antibodies, Anti-Idiotypic; D003470:Culture Media; D006133:Growth Substances; D007378:Interleukins; D008222:Lymphokines; D015847:Interleukin-4