title: Inhibition of the contraction of the isolated longitudinal muscle of the guinea-pig ileum by botulinum C2 toxin: evidence for a role of G/F-actin transition in smooth muscle contraction.
abstract: The effect of botulinum C2 toxin was studied on the contractions of the guinea pig ileum myenteric plexus longitudinal muscle preparation. Botulinum C2 toxin inhibited the muscle contraction induced by electrical stimulation (60 V; 0.5 ms; 0.33 Hz) in a time and concentration dependent manner. The inhibitory effect occurred with a time lag of about 1 h, and depended on the presence of both toxin components. After 4 h of incubation with 1.7 micrograms/ml of component I and 6.7 micrograms/ml of component II of botulinum C2 toxin, the smooth muscle contraction was inhibited by about 60%. At these toxin concentrations, about 55% of the modifiable smooth muscle actin was ADP-ribosylated. Smooth muscle contraction induced by bradykinin and bethanechol were similarly inhibited. Moreover, the C2 toxin inhibited muscle contraction induced by Ba2+, and by direct muscle membrane depolarization (60 V; 10 ms; 0.33 Hz) after suppression of acetylcholine release by normorphine. Also cytochalasin D inhibited the electrically evoked contraction of the ileum longitudinal muscle. In contrast to botulinum C2 toxin, inhibition of contractility by cytochalasin D occurred without a lag phase, and was reversed by washing off the toxin. In contrast of guinea pig ileum longitudinal muscle, botulinum C2 toxin did not reduce the contraction of the rabbit aortic smooth muscle stimulated by K+-depolarization or noradrenaline.
authors: Rudolf-Buchheim-Institut für Pharmakologie, Universität Giessen, Federal Republic of Germany.
authors: S
authors: S
authors: Mauss
authors: G
authors: G
authors: Koch
authors: V A
authors: VA
authors: Kreye
authors: K
authors: K
authors: Aktories
mesh_terms: D000199:Actins / Q000502:physiology*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D001626:Bethanechol Compounds / Q000494:pharmacology; D001905:Botulinum Toxins / Q000633:toxicity*; D001920:Bradykinin / Q000494:pharmacology; D004558:Electric Stimulation; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D011817:Rabbits; D013045:Species Specificity
chemical_list: D000199:Actins; D001626:Bethanechol Compounds; D000246:Adenosine Diphosphate Ribose; D001905:Botulinum Toxins; D001920:Bradykinin