A new cell culture assay for quality control in IVF.
A new, simple and sensitive bioassay for quality testing in IVF is described. This bioassay (Medi-Cult Hybritest, GEA Ltd, Biotech Division, Hvidovre, Denmark) is based on the culture of the rapidly growing mouse hybridoma cell line 1E6 in a defined serum-free culture medium. The use of serum-free conditions greatly increases the sensitivity to toxic substances, due to the absence of binding proteins. The testing of known toxic agents showed that this assay disclosed cytotoxicity with a high sensitivity. The Hybritest thus provides a simple yet sensitive and reproducible bioassay for quality control of culture media, water, chemical compounds and equipment in an IVF programme. Tests of different batches of culture media showed that media for IVF should be processed from powder and high-quality sterile water. It is important not only to test the single components of the culture media, but also the final product in a sensitive test system.
Bertheussen
K
K
Institute of Medical Biology, University of Tromsø, Norway.
Holst
N
N
Forsdahl
F
F
Høie
K E
KE
D000818:Animals; D002478:Cells, Cultured; D002594:Cetylpyridinium / Q000032:analysis; D003470:Culture Media / Q000032:analysis; D005307:Fertilization in Vitro; D005557:Formaldehyde / Q000032:analysis; D006825:Hybridomas; D006834:Hydrazines / Q000032:analysis; D066298:In Vitro Techniques; D008012:Lidocaine / Q000032:analysis; D051379:Mice; D011786:Quality Control; D014867:Water / Q000032:analysis
D003470:Culture Media; D006834:Hydrazines; D014867:Water; D005557:Formaldehyde; D008012:Lidocaine; D002594:Cetylpyridinium