The nucleotide sequence and characterization of four chloroplast tRNAs from the alga codium fragile.
The nucleotide sequences of four chloroplast tRNAs (methionine elongator, lysine, glycine, and arginine) from the siphonaceous green alga Codium fragile have been determined. These tRNAs have an unusually high A-U content compared to other chloroplast tRNAs and show varied, but in general only limited, sequence homology to the corresponding tRNAs of other chloroplasts. The locations of the genes for these four tRNAs have been determined and they show no similarity to the location of the corresponding tRNA genes in other chloroplasts. The Codium chloroplast glycine tRNA has an unmodified uridine in the wobble position of the anticodon, a characteristic rarely found in tRNA but present in mitochondrial tRNAs which read the genetic code by extended wobble.
Francis
M A
MA
Department of Biochemistry, State University of New York, Stony Brook 11794.
Suh
E R
ER
Dudock
B S
BS
D001483:Base Sequence; D000460:Chlorophyta / Q000235:genetics*; D002736:Chloroplasts / Q000032:analysis*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012347:RNA, Transfer, Arg / Q000235:genetics*; D012353:RNA, Transfer, Gly / Q000235:genetics*; D012357:RNA, Transfer, Lys / Q000235:genetics*; D012358:RNA, Transfer, Met / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D012345:RNA, Transfer, Amino Acid-Specific; D012347:RNA, Transfer, Arg; D012353:RNA, Transfer, Gly; D012357:RNA, Transfer, Lys; D012358:RNA, Transfer, Met