Joint inflammation induced by a mature macrophage cell line and cloned MT4+, Lyt-2- T cells in allogeneic mice.
Recently we reported the isolation of the mature macrophage cell line AP284. This line can efficiently present antigen to cloned helper T cells in vitro and in vivo. In this paper we show that AP284 can also present antigen to cloned helper T cells in an in vivo model system in which joint inflammation is induced. It appeared that injection of cloned helper T cells specific for methylated bovine serum albumin (mBSA) together with mBSA and AP284 into the joints of allogeneic mice induced a substantial joint inflammation. This system offers great prospects for studying the involvement of soluble mediators in the induction of joint inflammation as well as regulatory aspects of this inflammation.
Klasen
I S
IS
Department of Cell Biology, Immunology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Ladestein
R M
RM
Benner
R
R
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D004195:Disease Models, Animal; D007249:Inflammation / Q000276:immunology*; D007719:Knee Joint; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*