title: A second trans-spliced RNA leader sequence in the nematode Caenorhabditis elegans.
abstract: In the nematode Caenorhabditis elegans, the 22-nucleotide RNA sequence called the spliced leader (SL) is trans-spliced from the 100-nucleotide-long SL RNA to some mRNAs. We have identified a trans-spliced leader (SL2) whose sequence differs from that of the original spliced leader (SL1), although both are 22 nucleotides long. By primer-extension sequencing, SL2 but not SL1 was shown to be present at the 5' end of the mRNA encoded by one of the four glyceraldehyde-3-phosphate dehydrogenase genes. The other three glyceraldehyde-3-phosphate dehydrogenase genes encode mRNAs that have the SL1 but not the SL2 sequence at their 5' ends. Therefore, the trans-splicing process can discriminate the transfer of SL1 from that of SL2 in a gene-specific manner.
authors: Department of Developmental Biology, Synergen, Inc., Boulder, CO 80301.
authors: X Y
authors: XY
authors: Huang
authors: D
authors: D
authors: Hirsh
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002107:Caenorhabditis / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005796:Genes; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012333:RNA, Messenger; D004247:DNA