Inhibition by beta-thalassemic erythrocytes of erythropoiesis in vitro.
It is known that a medium conditioned by erythrocytes (ECM) reduces 59Fe uptake into haem in haemopoietic cell cultures. In order to evaluate whether the release of this factor in conditioned media is correlated with the whole erythrocyte surface, the same volume of packed erythrocytes of beta-thalassemic and normal subjects were incubated according to the method of Cole and Regan (1977). The influence of these conditioned media upon 59Fe uptake into haem in cultures of guinea pig bone marrow cells was studied. The results demonstrate that ECM of beta-thalassemic erythrocytes caused a more marked depression than ECM of normal erythrocytes, and that this inhibitory effect was correlated with the mean corpuscular volume (MCV) of the erythrocytes investigated.
Vedovato
M
M
Istituto di Anatomia Comparata, Università di Ferrara, Italy.
Salvatorelli
G
G
D002478:Cells, Cultured; D003470:Culture Media; D004912:Erythrocytes / Q000502:physiology*; D004920:Erythropoiesis; D006400:Hematocrit; D006801:Humans; D007501:Iron / Q000378:metabolism; D013789:Thalassemia / Q000097:blood*
D003470:Culture Media; D007501:Iron