Cloning of the potato virus Y genes encoding the capsid protein CP and the nuclear inclusion protein NIb.
DNA was synthesized complementary to the RNA genome of potato virus Y (PVY; strain GO 16) and cloned into lambda vectors. The size of PVY-specific Eco RI-restricted cDNA ranged from 0.3 to approximately 22kb. Two of the cDNA clones each of which contained some 4kb of cDNA sequence starting from the 3'-polyadenylated terminus were characterized by sequence analysis. Presence of a single open reading frame suggests that PVY-specific proteins are synthesized as a polyprotein precursor. As with other sequenced potyvirus RNAs the gene for the PVY capsid protein CP is located next to the 3'-untranslated region followed by the genes for the putative RNA polymerase (nuclear inclusion protein NIb) and the virus-specific protease (nuclear inclusion protein NIa). The 3'-trailing sequence of the PVY strains cloned is highly homologous to the corresponding region of pepper mottle virus (PeMV) and suggests that PeMV is not a distinct member of the potyvirus group, but another strain of PVY.
Wefels
E
E
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
Sommer
H
H
Salamini
F
F
Rohde
W
W
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D012367:RNA, Viral; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics*
D011498:Protein Precursors; D012367:RNA, Viral; D014764:Viral Proteins; C061396:nuclear inclusion protein b, mosaic viruses; D012321:DNA-Directed RNA Polymerases