Specific immunocytochemical visualization of phosphatidylserine.
In order to determine whether anti-phosphatidylserine antibodies are able to detect phosphatidylserine in situ, an immunocytochemical approach has been developed using human platelets. A strong positive reaction was obtained when anti-phosphatidylserine serum was applied to platelets whereas no reaction was observed with preimmune serum, or with immune serum which had been preincubated with 10(-5) M phosphatidylserine, suggesting that phosphatidylserine was indeed specifically detected by the antibodies.
Maneta-Peyret
L
L
I.B.C.N.-C.N.R.S., Bordeaux, France.
Freyburger
G
G
Bessoule
J J
JJ
Cassagne
C
C
D001792:Blood Platelets / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D010718:Phosphatidylserines / Q000032:analysis* / Q000276:immunology
D010718:Phosphatidylserines