Effects of serine protease inhibitors on oxyradical burst from phagocytizing neutrophils--analysis by chemiluminescence counting and its microscopic imaging.
Reaction difference of oxyradical generation and luminol-dependent photoemission of zymosan- and phorbol ester-treated neutrophils were investigated using a conventional photomultiplier and ultrasensitive photonic imaging technique. Zymosan-treated cells released a concentrated photonic burst corresponding to the cellular distribution. In contrast, phorbol ester-treated cells produced a negligible level of photoemission, and the additional application of Ca2+ ionophore enhanced the photonic burst, which was gradually spread out into extracellular space. Serine protease inhibitors did not attenuate PMA-induced chemiluminescence but did attenuate zymosan-induced chemiluminescence. This suggests the involvement of serine protease in the respiratory burst of phagocytizing neutrophils.
Suematsu
M
M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Houzawa
S
S
Miura
S
S
Nagata
H
H
Kitahora
T
T
Morishita
T
T
Oshio
C
C
Tsuchiya
M
M
D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010587:Phagocytosis; D015842:Serine Proteinase Inhibitors; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D005609:Free Radicals; D015842:Serine Proteinase Inhibitors; D008165:Luminol; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen