title: Failure to detect extremely high levels of serum IgE with an immunoradiometric assay.
abstract: Two patients with extreme elevations in serum IgE were found to have falsely low levels of IgE when measured by a commercial immunoradiometric assay. Dilution studies performed on serum from both patients revealed the rise and subsequent fall of the dose-response curve characteristic of the high-dose "hook" or prozone effect. Specimens with unexpectedly low levels of serum IgE obtained by either commercial immunoradiometric assay or enzyme immunoassay should be reanalyzed after dilution.
authors: Department of Allergy and Dermatology, Lahey Clinic Medical Center, Burlington, Massachusetts 01805.
authors: J A
authors: JA
authors: Saryan
authors: P E
authors: PE
authors: Garrett
authors: S R
authors: SR
authors: Kurtz
mesh_terms: D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic; D007073:Immunoglobulin E