Glutathione-coated cadmium-sulfide crystallites in Candida glabrata.
Cadmium-sulfide crystallites form in the yeast Candida glabrata cultured in the presence of cadmium salts. The particles function to sequester and detoxify intracellular cadmium ions. The crystallites are peptide-coated, but the coating peptide varies with the nutrient conditions of the growth medium. When cultured in rich nutrient broth the yeast forms intracellular CdS particles coated with a mixture of glutathione and the gamma-glutamylcysteine dipeptide. In contrast, cultures in synthetic minimal medium yield particles coated with polymerized gamma EC peptides of general structure (gamma-Glu-Cys)n-Gly. Glutathione/gamma-glutamylcysteine particles exhibit properties analogous to quantum, semiconductor-type crystallites. The optical properties are dependent on particle size, and irradiation results in photoluminescence and photoreduction not observed in bulk CdS mineral. Aerobic irradiation leads to particle decomposition presumably via oxidation of the sulfide ions within the crystallite.
Dameron
C T
CT
Department of Medicine, University of Utah Medical Center, Salt Lake City 84132.
Smith
B R
BR
Winge
D R
DR
D002104:Cadmium / Q000302:isolation & purification / Q000378:metabolism*; D019187:Cadmium Compounds; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D004151:Dipeptides / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D013053:Spectrophotometry; D013440:Sulfides; D013455:Sulfur / Q000032:analysis
D019187:Cadmium Compounds; D004151:Dipeptides; D013440:Sulfides; D002104:Cadmium; C034939:cadmium sulfide; D013455:Sulfur; D005978:Glutathione; C017341:gamma-glutamylcysteine