Control of beta-interferon expression in murine embryonal carcinoma F9 cells.
Murine embryonal carcinoma F9 cells, a tissue culture model for early embryonic development, do not produce interferon (IFN) in response to poly(I-C), as determined by an antiviral assay. RNase protection analyses were used to examine total RNA extracted from the cells for the presence of beta-IFN RNA. Whereas F9 cells differentiated in vitro with retinoic acid produced a biologically active protein as well as beta-IFN RNA in response to poly(I-C), undifferentiated F9 cells produced no detectable beta-IFN RNA even in the presence of cycloheximide, an IFN-superinducing agent. These results show that undifferentiated embryonal carcinoma cells do not accumulate beta-IFN RNA in response to an IFN-inducing agent, suggesting a transcriptional regulatory mechanism. However, this control mechanism is altered upon differentiation, since the gene can be transcriptionally activated in retinoic acid-differentiated cells.
Francis
M K
MK
Department of Microbiology and Immunology, Albany Medical College, New York 12208.
Lehman
J M
JM
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D054278:Embryonal Carcinoma Stem Cells; D005786:Gene Expression Regulation / Q000187:drug effects*; D007370:Interferon Type I / Q000235:genetics*; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D012260:Ribonucleases; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007370:Interferon Type I; D012333:RNA, Messenger; D014212:Tretinoin; D003513:Cycloheximide; D012260:Ribonucleases; D011070:Poly I-C