Dependence between the free-radical peroxidation, the activity of the superoxide dismutase glucose-6-phosphate dehydrogenase and erythrocytes haemolysis after thermal trauma and alpha-tocopherol treatment.
Experimental thermal trauma of IIIa-IIIb degree was induced on an area of 15-20% in male Wistar rats weighing 200-250 g. The changes in the content of the products of free-radical oxidation (FRO) - conjugated dienes and thiobarbituric acid (TBA) products in the blood, haemodialysis, the activity of superoxide dismutase (SOD) and of G-6-PHD-ase in the erythrocytes during the acute period after thermal trauma (24, 48 and 72 hours) were traced. The experimental data have shown that the percentage of haemolysed erythrocytes increases with the potentiation of FRO after burning. Intraperitoneal administration of alpha-tocopherol in a dose of 20 mg/kg body mass is followed by a reduction of the FRO products and by a rise in the antioxidative protection and resistance of the erythrocytes. The role of FRO in the pathogenesis of the damage of the erythrocytic membrane after thermal trauma and the protective effect of alpha-tocopherol are discussed.
Bekyarova
G
G
Higher Medical Institute, Varna.
Kozarev
I
I
Yankova
T
T
D000818:Animals; D002056:Burns / Q000097:blood* / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D005609:Free Radicals; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006400:Hematocrit; D006461:Hemolysis / Q000187:drug effects*; D008297:Male; D010545:Peroxides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
D005609:Free Radicals; D010545:Peroxides; D014810:Vitamin E; D005954:Glucosephosphate Dehydrogenase; D013482:Superoxide Dismutase