title: Changes in lens proteins induced at the early stage of cataractogenesis in cac (Nakano) mice.
abstract: Changes in lens proteins induced at the early stages of cataractogenesis in cac (Nakano) mice were investigated by two-dimensional gel electrophoresis (2D-PAGE). The 2D-PAGE profile of lens proteins in 21-day-old cac mice differed from that in 27-day-old normal mice, even though the appearance of 'pin-head' nuclear opacity (26-day-old) had not yet been observed in the lenses. Especially noticeable were great differences in the polypeptides associated with the alpha- and beta- crystallin subfractions, the appearances of which corresponded to an increase in a ratio of the amounts of Na+ to that of K+ in the lenses of defective mice. No dramatic decrease in the gamma-crystallin fraction was observed until the mature cataract stage.
authors: Kyoritsu College of Pharmacy, Tokyo, Japan.
authors: S
authors: S
authors: Kobayashi
authors: M
authors: M
authors: Kasuya
authors: M
authors: M
authors: Itoi
mesh_terms: D000818:Animals; D002386:Cataract / Q000209:etiology / Q000378:metabolism*; D003459:Crystallins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008817:Mice, Mutant Strains; D010455:Peptides / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D012995:Solubility
chemical_list: D003459:Crystallins; D010455:Peptides; D012964:Sodium; D011188:Potassium