Tumoricidal effect of interleukin-2-activated killer cells in canines.
We investigated the effect of both partially purified (TCGF) and recombinant interleukin-2 (rIL-2) on the tumor-directed cytotoxic activity of canine peripheral blood mononuclear cells (PBMC) using three normal canines. No cytotoxic activity was displayed by unstimulated effector cells at 3 h of incubation; however, the cytotoxic effect was observed in a 16-h assay. PBMC of all canines displayed significant levels of lytic activity after stimulation for 4 to 7 days with both types of IL-2 against a variety of allogeneic and xenogeneic neoplastic cells in 3-h 51Cr release assay. The cytotoxic activity of cultured cells increased proportionally in the 16-h assays. Morphological examination of the May-Grünwald and Giemsa stained cytocentrifuged slides of cultured cells on each day of assay showed an increase in large granular lymphocytes (LGLs) beginning on day 4 and reaching a peak on day 7 of culture.
Jardine
J H
JH
Department of Veterinary Medicine and Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Jackson
H J
HJ
Lotzová
E
E
Savary
C A
CA
Small
S M
SM
D000818:Animals; D003602:Cytotoxicity, Immunologic; D004285:Dogs / Q000276:immunology*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
D007376:Interleukin-2