Scanning tunneling and transmission electron microscopy on identical areas of biological specimens.
A methodology for tip and specimen-support manufacturing is described which allows for scanning tunneling microscopy (STM) and transmission electron microscopy (TEM) on identical areas of biological specimens, at comparable resolution. Topographs and dI/ds-maps were used to investigate tip-specimen interaction on native air-dried phage T4 polyheads where individual capsomeres and structural alterations upon repetitive scans have been observed at a tunnel current of 0.5 pA.
Stemmer
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M.E. Müller Institute for High Resolution Electron Microscopy at the Biocenter, University of Basel, Switzerland.
Hefti
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Aebi
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U
Engel
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D008855:Microscopy, Electron, Scanning / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D013604:T-Phages / Q000648:ultrastructure