Effects of calcium channel blockers on the endothelial release of von Willebrand factor after exercise in healthy subjects.
Von Willebrand factor (vWF) is known to play a relevant role in the development of atherosclerotic lesions by promoting platelet adhesion to injured endothelial cells. An increase in cytosolic calcium has been shown to be associated with the in vitro release of vWF from endothelial cells. The aim of our investigation was to examine the effect of some calcium channel blockers on the release of vWF antigen (vWF:Ag) induced by exercise in two groups of eight healthy subjects during a randomized crossover study between placebo and calcium channel blockers. Placebo and verapamil (80 mg) or nicardipine (10 mg) were given orally at an interval of 2 weeks three times on the day before and once on the morning of the study day. Measurements of plasma vWF:Ag were made at rest and after a progressive maximal exercise on a cycloergometer. A significant increase in absolute values of vWF:Ag was observed after exercise in the subjects given a placebo in both verapamil (0.397 +/- 0.074 U/ml) and nicardipine (0.327 +/- 0.036 U/ml) groups, p less than 0.05. With use of verapamil the rise in vWF:Ag was blunted and not significant (0.123 +/- 0.081 U/ml) whereas a larger increase in vWF:Ag was found in the subjects given nicardipine in comparison with placebo (0.593 +/- 120 U/ml; p less than 0.05). No correlations were observed between exercise-induced changes in systolic or diastolic blood pressure, heart rate, blood lactate level, or pH and vWF:Ag changes in the subjects given a placebo or in those given calcium channel blockers.(ABSTRACT TRUNCATED AT 250 WORDS)
Musumeci
V
V
Istituto di Patologia Speciale Medica Università Cattolica del Sacro Cuore Rome, Italy.
Cardillo
C
C
Baroni
S
S
Zuppi
C
C
Zappacosta
B
B
Tutinelli
F
F
Folli
G
G
D000328:Adult; D000941:Antigens / Q000032:analysis; D001769:Blood; D001794:Blood Pressure; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D015444:Exercise; D006339:Heart Rate; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008297:Male; D009529:Nicardipine / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology*; D014841:von Willebrand Factor / Q000276:immunology / Q000378:metabolism*
D000941:Antigens; D007773:Lactates; C029417:Von Willebrand antigen; D014841:von Willebrand Factor; D019344:Lactic Acid; D014700:Verapamil; D009529:Nicardipine