title: Coexistence of delta sleep-inducing peptide and serotonin in midgut carcinoid tumour cells in vivo and in vitro.
abstract: By immunocytochemistry, delta sleep-inducing peptide (DSIP) was demonstrated to coexist with serotonin (5-HT) in a majority of midgut carcinoid tumour cells studied in biopsies and long-term cell cultures. Tumour cell colonies were characterized ultrastructurally and by confocal laser microscopy. The cultures produced several DSIP-like peptides chromatographically separated from culture media. DSIP has not yet proved to be a useful tumour marker clinically. Provocation with pentagastrin in patients with midgut carcinoid syndrome resulted in increased peripheral levels of 5HT, but not of DSIP.
authors: Department of Surgery, University of Göteborg, Sweden.
authors: H
authors: H
authors: Ahlman
authors: L
authors: L
authors: Ahlund
authors: O
authors: O
authors: Nilsson
authors: A
authors: A
authors: Dahlström
authors: A
authors: A
authors: Bjartell
authors: R
authors: R
authors: Ekman
mesh_terms: D001706:Biopsy; D002276:Carcinoid Tumor / Q000378:metabolism* / Q000648:ultrastructure; D003701:Delta Sleep-Inducing Peptide / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007414:Intestinal Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D008198:Lymph Nodes / Q000378:metabolism / Q000648:ultrastructure; D008207:Lymphatic Metastasis; D010418:Pentagastrin; D012701:Serotonin / Q000032:analysis / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D012701:Serotonin; D003701:Delta Sleep-Inducing Peptide; D010418:Pentagastrin