Automation and computerization of chemiluminescence: a new methodological approach in the study of human phagocytes.
Peripheral blood phagocytic cells (PMNLs) are activated by contact with opsonized particles. Metabolic activation of PMNLs is associated with a remarkable increase in the respiratory burst and generates high energy oxygen compounds which are responsible for the bactericidal activity of PMNLs and for their ability to produce luminol-dependent chemiluminescence (CL). The CL phenomenon is measured by an automated and computerized photoluminometer (Berthold LB950) in whole blood stimulated with opsonized zymosan. This whole blood method of CL measurement has been applied to the study of the phagocytic process and to the investigation of cellular and humoral abnormalities in several pathologies, indicating this assay as a simple, rapid and reliable test.
Parente
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Clinical Laboratory, M. Malpighi Hospital, Bologna, Italy.
Melotti
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D000328:Adult; D002648:Child; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009504:Neutrophils / Q000502:physiology*; D009895:Opsonin Proteins; D010100:Oxygen / Q000378:metabolism; D010587:Phagocytosis; D012016:Reference Values; D015054:Zymosan
D009895:Opsonin Proteins; D015054:Zymosan; D010100:Oxygen