Quantitation of HIV-1 RNA in blood cells of ARC and AIDS patients.
Peripheral blood mononuclear cells from HIV-1-infected persons were mixed with 5 M guanidine thiocyanate, and HIV-1-specific probes were hybridized with target RNA directly in the lysate. No RNA purification was needed. Hybrids were purified by repeated capture on superparamagnetic beads coated with oligo(dT) in a device-assisted format, a procedure termed "reversible target capture." Blood mononuclear cells from 70 ARC and AIDS patients were examined and found to have an average of 1.8 x 10(5) molecules of HIV-1 RNA per 2 x 10(6) cells.
Solomon
R
R
Department of Neoplastic Diseases, Hahnemann University, Philadelphia, Pennsylvania 19102.
Thompson
J
J
Gillespie
D
D
D000386:AIDS-Related Complex / Q000382:microbiology*; D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D006146:Guanidines; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000032:analysis* / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012367:RNA, Viral / Q000097:blood*; D013861:Thiocyanates
D006146:Guanidines; D015347:RNA Probes; D012367:RNA, Viral; D013861:Thiocyanates; C054436:guanidine thiocyanate