In vivo studies on the effects of human recombinant interleukin-1 beta on articular cartilage.
Interleukin-1 (IL-1) is a cytokine produced by a number of connective-tissue and inflammatory cells which has been shown in organ culture to stimulate the breakdown of cartilage proteoglycans and inhibit their synthesis. Intraarticular injection of human recombinant IL-1 beta into the knee joints of rabbits induced a dose-related decrease in cartilage proteoglycan content and increased infiltration of cells into the synovial fluid. Following a single intraarticular injection, the loss of proteoglycan was maximal at 3 days. By 7 days, proteoglycan content began to return toward control levels. IL-1 also resulted in a dose-related decrease in the ability of cartilage to synthesize new proteoglycan as measured by 35S incorporation. These in vivo effects of IL-1 on articular cartilage closely reflect those effects observed in vitro in organ culture and are consistent with the hypothesis that IL-1 may play a role as a mediator of the loss of cartilage in some arthritic diseases.
Arner
E C
EC
Medical Products Department E. I. Du Pont de Nemours & Co., Wilmington, De 19880-0400.
Di Meo
T M
TM
Ruhl
D M
DM
Pratta
M A
MA
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects*; D015232:Dinoprostone / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011509:Proteoglycans / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D013462:Sulfur Radioisotopes; D013582:Synovial Fluid / Q000166:cytology / Q000187:drug effects; D013997:Time Factors
D007375:Interleukin-1; D011509:Proteoglycans; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D015232:Dinoprostone