Analysis of the origin of marrow cells in bone marrow transplant recipients using a Y-chromosome-specific in situ hybridization assay.
A Y-chromosome-specific in situ hybridization assay was used to assess the frequency with which host bone marrow cells are retained after marrow grafting. The majority of patients (74%) showed the presence of both host and donor marrow cells when assayed 14 days after transplant. By 84 days posttransplant only 4% of the patients retained host marrow cells. Only 1 of 19 evaluable patients analyzed over 1 year posttransplant showed minimal retention of host cells. No statistical correlation was found between retention of host cells posttransplant and the development of relapse or acute or chronic graft-versus-host disease. Pretransplant conditioning regimen, HLA-matching, diagnosis, disease status at transplant, ABO-matching, and patient age also showed no correlation with the retention of host cells posttransplant.
Durnam
D M
DM
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Anders
K R
KR
Fisher
L
L
O'Quigley
J
J
Bryant
E M
EM
Thomas
E D
ED
D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000473:pathology*; D002678:Chimera; D005260:Female; D006085:Graft Survival; D006801:Humans; D007938:Leukemia / Q000601:surgery; D008297:Male; D009693:Nucleic Acid Hybridization; D013997:Time Factors; D014998:Y Chromosome