Interface-mediated inactivation of pancreatic lipase by a water-reactive compound: 2-sulfobenzoic cyclic anhydride.
2-Sulfobenzoic cyclic anhydride (SBA) rapidly and selectively inactivates porcine pancreatic lipase (PPL) only when added during the hydrolysis of an emulsified ester such as tributyrin or dodecyl acetate. The present data suggest that the inactivation of PPL occurs preferentially at the oil/water interface and not in the aqueous phase, since colipase and bile salt were found to adversely affect the inhibition process. Moreover, it is shown that at a molar ratio of SBA to pure PPL of 1, 40% of the lipase activity was already irreversibly lost. Complete inactivation was observed at SBA to pure PPL molar ratios of 120. A 60% inactivation occurred when 0.5 mol of 3H-labeled SBA was attached per mole of PPL. The SBA-inactivated PPL competes for binding to the dodecyl acetate/water interface as efficiently as the native enzyme. Larger SBA concentrations are required when crude lipase preparations are used as well as with pure PPL in the presence of bile salts and colipase. Lipases were found to have variable sensitivities to SBA inactivation, depending on their origin. In the presence of bile salts and tributyrin at pH 6.0, human gastric lipase activity was not affected by the presence of a 10(6) molar excess of SBA.
Moulin
A
A
Centre de Biochimie et de Biologie Moléculaire du CNRS, Marseille, France.
Fourneron
J D
JD
Piéroni
G
G
Verger
R
R
D000818:Animals; D001565:Benzoates / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D003089:Colipases / Q000494:pharmacology; D004655:Emulsions; D007700:Kinetics; D008049:Lipase / Q000037:antagonists & inhibitors*; D010179:Pancreas / Q000201:enzymology*; D011485:Protein Binding; D013499:Surface Properties; D013552:Swine; D013657:Taurodeoxycholic Acid / Q000494:pharmacology
D001565:Benzoates; D003089:Colipases; D004655:Emulsions; D013657:Taurodeoxycholic Acid; C028022:2-sulfobenzoic acid cyclic anhydride; D008049:Lipase