Negative functional interference between two active centers is indicated in animal fatty acid synthetase.
When fatty acid synthetase of the Harderian gland of guinea-pig was treated with various amounts of phenylmethanesulfonyl fluoride, the overall activity of the enzyme showed a quadratic decrease with respect to the inhibition degree of the thioesterase activity which was the primary target of inhibition. Moreover, the overall activity per active center of a heterodimer, which was formed between the native monomer and the thioesterase-less monomer, was higher than that of the native enzyme. These results are consistent with the view that the two active centers of the native enzyme exhibit a negative interference to each other.
Kyushiki
H
H
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Ikai
A
A
D000818:Animals; D001665:Binding Sites; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006168:Guinea Pigs; D006243:Harderian Gland / Q000201:enzymology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D013450:Sulfones / Q000494:pharmacology*
D013450:Sulfones; D010664:Phenylmethylsulfonyl Fluoride; D064429:Fatty Acid Synthases