Inhibition of neutrophil adherence to endothelial cells by 3-deazaadenosine.
Treatment of cultured human umbilical vein endothelial cells (HUVE) with tumor necrosis factor alpha (TNF-alpha) increases their capacity to adhere human neutrophils. We have found that 3-deazaadenosine (c3Ado), when added in conjunction with TNF-alpha, inhibited this increase in neutrophil adherence. This activity of c3Ado was potentiated by the addition of L-homocysteine thiolactone (Hcy). The ability of c3Ado to inhibit neutrophil adherence to HUVE may contribute to the anti-inflammatory activity of this nucleoside analogue.
Jurgensen
C H
CH
Wellcome Research Laboratories, Research Triangle Park, NC 27709.
Wolberg
G
G
Zimmerman
T P
TP
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D007957:Leukocyte Adherence Inhibition Test; D009504:Neutrophils / Q000187:drug effects*; D014372:Tubercidin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D014409:Tumor Necrosis Factor-alpha; C018258:3-deazaadenosine; D014372:Tubercidin