[The study of degradation of peptides in human plasma and serum by the ERIAD high pressure liquid chromatography-mass spectrometry method].
The proteolysis of bradykinin, [Leu5]enkephalin and some of its synthetic analogs. [D-Ala2,Leu5]enkephalin and [D-Ala2,Leu5]enkephalinyl-Arg, by human blood plasma and serum enzymes was investigated. The degradation products were identified. Based on the kinetic data, the principal pathways of degradation and its limiting steps were established.
Mirgorodskaia
O A
OA
Podtelezhnikov
A V
AV
Shevchenko
A A
AA
D001920:Bradykinin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004745:Enkephalins / Q000097:blood*; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods
D004745:Enkephalins; D001920:Bradykinin
Izuchenie degradatsii peptidov v plazme i syvorotke krove cheloveka metodom vysokoéffektivnoĭ zhidkostnoĭ khromatografii-mass- spektrometrii ERIAD.