title: Multilayer coil planet centrifuge for analytical high-speed counter-current chromatography.
abstract: A compact portable model of a high-speed counter-current chromatograph enables efficient analytical separations of microgram sample quantities within 10 min. A series of preliminary experiments was conducted to study retention of the stationary phase of various two-phase solvent systems in short coils with different helical diameters. Under the optimal experimental conditions, analytical capability of the apparatus was successfully demonstrated in separation of flavonoids from a crude sea buckthorn ethanol extract in a multilayer coil with a total capacity of 8 ml.
authors: Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
authors: H
authors: H
authors: Oka
authors: F
authors: F
authors: Oka
authors: Y
authors: Y
authors: Ito
mesh_terms: D002498:Centrifugation / Q000295:instrumentation*; D002845:Chromatography / Q000295:instrumentation*