Identification of a novel site specific endonuclease produced by Mycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines.
We have discovered a new restriction endonuclease, MfeI, in nuclear extracts from T cells contaminated with Mycoplasma fermentans. This endonuclease was identified while studying proteins binding to the interleukin-2 receptor alpha chain gene promoter. MfeI cuts at the recognition sequence C'AATTG generating EcoRI compatible cohesive ends. Potential applications are discussed.
Halden
N F
NF
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Wolf
J B
JB
Leonard
W J
WJ
D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D013379:Substrate Specificity; D013601:T-Lymphocytes / Q000201:enzymology*
D004268:DNA-Binding Proteins; C059776:endodeoxyribonuclease MfeI; D015252:Deoxyribonucleases, Type II Site-Specific