Granulomatous and nongranulomatous lymphadenitis in sarcoidosis. An immunophenotypic study of seven cases.
Five cases of prescalenic granulomatous lymphadenitis (GL) and 2 cases of prescalenic nongranulomatous lymphadenitis (NGL) in 7 patients with sarcoidosis were studied with a large panel of monoclonal antibodies (anti-CD 1, CD 3, CD 4, CD 8, CD 14, CD 22, CD 25, HLA-DR, HLA-DQ, HNK-1, R 4/23). Immunopathologic analysis was performed by studying three different compartments of GL--granuloma, intergranulomatous area and sinuses--and of NGL--cortex, paracortex and sinuses. Intra- and intergranulomatous T lymphocytes were mainly of the CD 4 type in 4 out of 5 cases; in all the cases less than 25% of T lymphocytes stained also for CD 25. Epithelioid cells were HLA-DR+, HLA-DQ+, CD 14+ and, frequently, CD 1+ and CD 25+, the latter positivity being mainly restricted to the marginally located epithelioid cells. Sinuses were filled with T- and B- cells; sinusal histiocytes were HLA-DR+, HLA-DQ+, CD 14+ and, frequently, CD 1+ and CD 25+. In the cortex and paracortex of NGL, T-cell subsets paralleled the distribution and ratio observed in the intergranulomatous area of GL; furthermore the immunophenotype of NGL sinusal histiocytes roughly overlapped that observed in the same district of GL with a strong CD 1 and CD 25 positivity. These results, besides confirming the global imbalance of the CD 4/8 ratio in all the areas of GL, seem to demonstrate that the prevalence of CD 4+ or CD 8+ T-cells probably reflects different functional phases of the granulomatous process.(ABSTRACT TRUNCATED AT 250 WORDS)
Roncalli
M
M
2nd Department of Pathology, University of Milan, Italy.
Servida
E
E
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D002540:Cerebral Cortex / Q000276:immunology / Q000473:pathology; D006099:Granuloma / Q000150:complications* / Q000276:immunology / Q000473:pathology; D006680:HLA Antigens / Q000032:analysis / Q000145:classification; D006801:Humans; D008199:Lymphadenitis / Q000150:complications* / Q000276:immunology / Q000473:pathology; D010641:Phenotype; D012507:Sarcoidosis / Q000150:complications* / Q000276:immunology / Q000473:pathology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006680:HLA Antigens