Evaluation of the enhanced rapid identification method for Gardnerella vaginalis.
The enhanced rapid identification method (RIM; Austin Biological Laboratories), a micromethod for the identification of Gardnerella vaginalis, is based on starch and raffinose fermentation and hippurate hydrolysis. We tested 105 clinical isolates of G. vaginalis with both the RIM and standard biochemical tests. The RIM agreed with the standard biochemical methods for 96 (91.4%) of the strains; nine isolates which were hippurate hydrolysis positive by standard biochemical tests were hippurate hydrolysis negative in the RIM. RIM may serve as a useful adjunct to Gram stain and colony morphology for the identification of G. vaginalis.
Lien
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EA
Department of Obstetrics and Gynecology, University of Washington, Seattle 98195.
Hillier
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SL
D005260:Female; D005285:Fermentation; D006194:Gardnerella vaginalis / Q000302:isolation & purification* / Q000378:metabolism; D006190:Haemophilus / Q000302:isolation & purification*; D006626:Hippurates / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D011237:Predictive Value of Tests; D011887:Raffinose / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D013213:Starch / Q000378:metabolism; D013997:Time Factors; D014621:Vagina / Q000382:microbiology*
D006626:Hippurates; D011933:Reagent Kits, Diagnostic; D013213:Starch; D011887:Raffinose