Kaolin-induced writhing in mice, a new model of possible bradykinin-induced pain for assessment of analgesic agents.
Kaolin induced a clear and reproducible writhing reaction when intraperitoneally injected into mice. A simultaneous injection (i.p.) of soybean trypsin inhibitor (SBTI) significantly suppressed the kaolin-induced writhing reaction. This writhing reaction was markedly potentiated by a simultaneous injection (i.p.) of captopril. In an in vitro experiment kaolin caused kinin-release in mouse plasma, possibly through the activation of prekallikrein. This activation of plasma prekallikrein and kinin-release were inhibited in the presence of SBTI. Some non-steroidal anti-inflammatory agents inhibited the kaolin-induced writhing reaction dose-dependently. These results suggest that kaolin-induced writhing reaction may be caused by the released bradykinin through activation of the plasma kallikrein-kinin system. This model is a novel and simple tool for assessment of analgesic agents.
Fujiyoshi
T
T
Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Hayashi
I
I
Ohishi
S
S
Kuwashima
M
M
Iida
H
H
Dozen
M
M
Taniguchi
N
N
Ikeda
K
K
Ohnishi
H
H
D000109:Acetylcholine; D000700:Analgesics / Q000627:therapeutic use*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D016227:Benzoquinones; D001920:Bradykinin / Q000502:physiology*; D004195:Disease Models, Animal; D007616:Kaolin; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000139:chemically induced* / Q000188:drug therapy; D011809:Quinones
D000700:Analgesics; D016227:Benzoquinones; D011809:Quinones; D007616:Kaolin; C029322:phenylbenzoquinone; D000109:Acetylcholine; D001920:Bradykinin