Molecular and cellular studies of tryptophanyl-tRNA synthetases using monoclonal antibodies. Remarkable variations in the content of tryptophanyl-tRNA synthetase in the pancreas of different mammals.
The content of Trp-tRNA synthetase in pancreas and liver of cattle, sheep, swine, rat, rabbit and man was assayed by direct radioimmunoblotting with a 125I-labelled monoclonal antibody Am1, specifically interacting with any eukaryotic Trp-tRNA synthetase. Its content in the organs studied, with the exception of bovine and sheep pancreas, was found to be 0.002-0.012% of total proteins. The enzyme content in bovine pancreas was about 0.2% of total proteins, i.e. 70 times higher than in bovine liver; similar correlations were found for sheep. The Trp-tRNA synthetase levels in each organ varied from animal to animal of the same species by not more than a factor of four; these individual variations cannot affect the conclusion about the profound differences in the levels of the enzyme in pancreases of Ruminantia and of the other mammalians. As shown by indirect immunofluorescence technique, bovine Trp-tRNA synthetase is mainly located in the exocrine part of the pancreas. Moreover, the immunoreactive material is detectable also in bovine (not human) pancreatic juice. The abnormally high Trp-tRNA synthetase content in the ruminant pancreas may be connected with unknown function(s) of this protein somehow related to the peculiarities of digestion of these mammals.
Favorova
O O
OO
V. Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Zargarova
T A
TA
Rukosuyev
V S
VS
Beresten
S F
SF
Kisselev
L L
LL
D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D006801:Humans; D015151:Immunoblotting; D008099:Liver / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology*; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D014369:Tryptophan-tRNA Ligase / Q000032:analysis* / Q000502:physiology
D000911:Antibodies, Monoclonal; D000604:Amino Acyl-tRNA Synthetases; D014369:Tryptophan-tRNA Ligase