Identification of a novel metabolite in phenanthrene metabolism by the fungus Cunninghamella elegans.
The metabolism of phenanthrene by the fungus Cunninghamella elegans was investigated. Kinetic experiments using [9-14C]phenanthrene showed that after 72 h, 53% of the total radioactivity was associated with a glucoside conjugate of 1-hydroxyphenanthrene (phenanthrene 1-O-beta-glucose). This metabolite was isolated by reversed-phase high-performance liquid chromatography and characterized by the application of UV absorption, 1H nuclear magnetic resonance, and mass spectral techniques. The results show that aromatic ring oxidation followed by glucosylation is a predominant pathway in the metabolism of the polycyclic aromatic hydrocarbon phenanthrene by C. elegans.
Cerniglia
C E
CE
National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079.
Campbell
W L
WL
Freeman
J P
JP
Evans
F E
FE
D000107:Acetylation; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D009090:Mucorales / Q000378:metabolism*; D010616:Phenanthrenes / Q000302:isolation & purification / Q000378:metabolism*
D010616:Phenanthrenes