[Effect of nitrobenzylthioinosine accumulated by the heart in rats and guinea pigs on the release of adenine nucleotide breakdown products during ischemia and reperfusion].
The uptake kinetics of nitrobenzyl thioinosine (NBTI), a nucleoside transport inhibitor, was studied in the isolated Langendorf-perfused guinea pig and rat hearts. In rats the rate constant of NBTI uptake was higher and the extent of NBTI accumulation was less than in guinea pig hearts. Heart-accumulated NBTI inhibited the total release of adenine nucleotide degradation products (ANDP) during reperfusion 25 min after global ischemia. The effect was more pronounced in guinea-pig hearts-in accordance with observed higher myocardial concentration of NBTI. Unlike other ANDP, the release adenosine by guinea-pig hearts was unchanged and that by rat hearts increased. In spite of significant NBTI-induced decrease of ANDP losses recovery of contractile function during reperfusion was not observed to improve.
Kuz'min
A I
AI
Shul'zhenko
V S
VS
Medvedev
O S
OS
Kapel'ko
V I
VI
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D003327:Coronary Disease / Q000378:metabolism*; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion / Q000379:methods; D051381:Rats; D013868:Thioinosine / Q000493:pharmacokinetics / Q000494:pharmacology / Q000031:analogs & derivatives; D013997:Time Factors
D000227:Adenine Nucleotides; D013868:Thioinosine; C001789:4-nitrobenzylthioinosine
Vliianie nitrobenziltioinozina, nakoplennogo serdtsem krys i morskikh svinok, na vysvobozhdenie produktov raspada adeninnukleotidov pri ishemii i reperfuzii.