Suppression of cytotoxic T cell induction in vivo by prodigiosin 25-C.
Prodigiosin 25-C (PrG25-C) was discovered as an immunosuppressant in the course of our screening for immunomodulating substances. In this system, PrG25-C inhibited T lymphocytes proliferation and was less suppressive against B lymphocytes. PrG25-C was also a powerful inhibitor of cytotoxic T cell induction by mixed lymphocyte reaction and completely suppressed induction of H-2 specific cytotoxic cells at 12.7 nM. PrG25-C also inhibited in vivo induction of H-2 restricted cytotoxic T lymphocytes at a dose of 0.5 mg/kg but had little myelotoxicity because numbers of blood leukocytes and splenocytes of PrG25-C-treated mice were comparable to those of nonsensitized mice. No inhibitory effects of PrG25-C were observed on the production of anti-SRBC antibody. These results indicate that PrG25-C is a T-lymphocyte-specific immunosuppressant.
Nakamura
A
A
Department of Agricultural Chemistry, University of Tokyo, Japan.
Magae
J
J
Tsuji
R F
RF
Yamasaki
M
M
Nagai
K
K
D000818:Animals; D000961:Antilymphocyte Serum / Q000096:biosynthesis; D002478:Cells, Cultured; D003524:Cyclosporins / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011353:Prodigiosin / Q000008:administration & dosage* / Q000494:pharmacology; D013154:Spleen; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects*
D000961:Antilymphocyte Serum; D003524:Cyclosporins; D007166:Immunosuppressive Agents; D011353:Prodigiosin