Characterization of elastin protein and mRNA from salmonid fish (Oncorhynchus kisutch).
1. Elastin was isolated from the bulbus arteriosus of a salmonid fish. Monoclonal and polyclonal antibodies, elicited against a CNBr digest of this protein, immunoprecipitated a polypeptide of Mr 43,000 from fish cell culture medium. 2. Cell-free translation of salmon poly A+ RNA produced a protein of approximately 43 kD that was immunoprecipitated with anti-elastin antibodies. The corresponding mRNA had an approximate Mr of 2 kb. 3. Despite similarities in amino acid composition, the differences in Mr between mammalian and salmon mRNA and protein suggest a divergence of fish and higher vertebrate elastins from an earlier ancestral gene.
Chow
M
M
Department of Biological Structure, University of Washington, Seattle 98195.
Boyd
C D
CD
Iruela-Arispe
M L
ML
Wrenn
D S
DS
Mecham
R
R
Sage
E H
EH
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002474:Cell-Free System; D004549:Elastin / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012474:Salmon / Q000502:physiology*; D014333:Tropoelastin / Q000276:immunology
D000596:Amino Acids; D000906:Antibodies; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D012333:RNA, Messenger; D014333:Tropoelastin; D004549:Elastin