The cold-induced denaturation of lactate dehydrogenase at sub-zero temperatures in the absence of perturbants.
The cold-induced denaturation of lactate dehydrogenase has been determined in an unfrozen, cryoprotectant free solution at sub-zero temperatures. The cold-induced denaturation temperature (TL) has been found to be -28 degrees C. The results for the first time clearly establish that temperature alone can induce denaturation in a cooled protein solution. The validity of earlier data, obtained in the presence of perturbants (particularly pH or guanidinium chloride), is discussed.
Hatley
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RH
Pafra Ltd, Biopreservation Division, Cambridge, England.
Franks
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D000818:Animals; D003080:Cold Temperature; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology; D011489:Protein Denaturation; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D007770:L-Lactate Dehydrogenase