Fodrin and band 4.1 in a plasma membrane-associated fraction of human neutrophils.
Erythrocytes possess a well-characterized submembranous filamentous network which interacts with transmembrane glycoproteins and is composed primarily of spectrin, ankyrin, band 4.1, and short actin filaments. An analogous structure was recently described in platelets. Human polymorphonuclear leukocytes (PMNs) were examined for the presence and plasma membrane association of similar proteins. Isolated PMNs, free of contamination with erythrocytes or platelets, were disrupted by nitrogen cavitation and separated into subcellular organelles on a discontinuous Percoll gradient. Detergent lysates of plasma membrane vesicles, but not azurophilic or specific granules, contained insoluble actin filaments and associated proteins. Immunoblots of detergent-insoluble plasma membrane fractions contained proteins recognized by antibodies to brain fodrin and erythrocyte band 4.1, whereas blots probed with antibodies to erythrocyte spectrin and ankyrin were negative. Fodrin and band 4.1 were not detected in granule fractions, but some fodrin was present in the cytosol. The association of proteins related to fodrin and band 4.1 with the plasma membrane suggests that PMNs contain a submembranous skeleton structurally analogous to that of erythrocytes and platelets. The specific function of these proteins and their structural organization in human PMNs await further study.
Stevenson
K B
KB
Department of Medicine, Veterans Administration Medical Center, Iowa City, IA.
Clark
R A
RA
Nauseef
W M
WM
D008841:Actin Cytoskeleton / Q000502:physiology / Q000648:ultrastructure; D000199:Actins / Q000502:physiology*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000502:physiology*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D008562:Membrane Glycoproteins / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology*; D008840:Microfilament Proteins / Q000502:physiology*; D008970:Molecular Weight; D009479:Neuropeptides; D009504:Neutrophils / Q000648:ultrastructure*; D013049:Spectrin
D000199:Actins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009479:Neuropeptides; D013049:Spectrin; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1