Loss of interleukin 2 dependence in cloned interleukin 2-dependent rat T lymphocyte x BW5147 hybridomas is not associated with segregation of a specific pair of rat chromosomes.
A fusion between the mouse AKR thymoma BW5147 and a culture of homogeneously OX8+ (CD8) rat T lymphoblasts yield interleukin (IL) 2-dependent T cell hybridomas when selected in HAT medium supplemented with IL 2-containing supernatants of concanvalin A-activated cells and dexamethasone. IL 2-independent variants can be selected from cloned IL2-dependent hybrids in the absence of conditioned medium. Karyotype analysis was used to test a previously proposed hypothesis according to which IL2-independent variants arise through loss of a specific cytotoxic T lymphocyte (rat) chromosome carrying a gene responsible for IL2 dependence. Comparison of karyotypes of several independently derived hybrids with those of their IL 2-independent variants showed that the hybrids contain at least one homologue of all rat chromosomes, and that no pair of rat chromosomes is consistently absent in the IL 2-independent variants.
Somoza
C
C
Department of Immunology, Clínica Puerta de Hierro, Madrid, Spain.
Fernández-Ruiz
E
E
Jotterand-Bellomo
M
M
Sanz
E
E
Nabholz
M
M
Silva
A
A
D000818:Animals; D002455:Cell Division; D002871:Chromosome Banding; D006825:Hybridomas / Q000502:physiology* / Q000648:ultrastructure; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D010641:Phenotype; D051381:Rats; D013601:T-Lymphocytes / Q000502:physiology*
D007376:Interleukin-2