Enzyme-linked immunosorbent assay for the detection of circulating antigens of Toxoplasma gondii in the serum of cats.
An ELISA was developed to detect circulating antigens of Toxoplasma gondii in the serum of cats. For the experiment, toxoplasmosis was induced in a group of cats by oral administration of bradyzoites. An ELISA that detects anti-Toxoplasma IgG, an ELISA to detect circulating antigens, and fecal examinations were performed on samples from each cat for 1 year after inoculation. When coupled with IgG-class antibody measurement, antigen detection can aid in the diagnosis of some cases of subclinical feline toxoplasmosis.
Lappin
M R
MR
Department of Small Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
Greene
C E
CE
Prestwood
A K
AK
Dawe
D L
DL
Tarleton
R L
RL
D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D002371:Cat Diseases / Q000175:diagnosis* / Q000276:immunology; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D013045:Species Specificity; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000276:immunology
D000953:Antigens, Protozoan