Enhancement of HIV replication and giant cell formation by tumor necrosis factor.
Human T cell lines Molt-4, Jurkat, and TLOm-1 were infected by HIV-1 in the presence of various concentrations of r-TNF. The infectivity of HIV-1 was monitored by an indirect immunofluorescence method using anti-HIV-1-positive human serum. We found that r-TNF enhanced the replication of HIV-1. HIV-1-induced giant cell formation between HIV-1 chronically infected Molt-4 cells and HIV-1-uninfected Molt-4 cells was accelerated by r-TNF. The median tissue culture infectious doses (TCID50) of HIV-1 determined in the presence of TNF revealed that TNF apparently accelerated the time of the appearance of CPE but did not affect final titer of HIV-1.
Matsuyama
T
T
Department of Virology and Parasitology, School of Medicine, University of Yamaguchi, Japan.
Yoshiyama
H
H
Hamamoto
Y
Y
Yamamoto
N
N
Soma
G
G
Mizuno
D
D
Kobayashi
N
N
D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D014409:Tumor Necrosis Factor-alpha