T lymphocytes in infectious mononucleosis; effect of IL-2 on the outgrowth of Epstein-Barr virus-infected cells.
The addition of interleukin-2 (IL-2) to lymphocyte cultures from acute infectious mononucleosis (IM) donors dramatically increased the incidence of regression in such cultures and resulted in the emergence of an IL-2 dependent, CD3 Epstein-Barr virus nuclear antigen (EBNA)-negative cell population. Corresponding cultures seeded in the absence of IL-2 rarely regressed and were quickly dominated by IL-2 independent, CD3-negative, EBNA-positive cells. Lymphocyte cultures from Epstein-Barr virus (EBV) seropositive donors showed enhanced regression in the presence of IL-2 but failed to regress after the removal of the E-rosetting population. Cultures from EBV-seronegative donors showed no evidence of regression in the presence or absence of IL-2. E-rosetting cells isolated from cultures from acute IM donors that had been cultured in the presence of IL-2 lysed autologous and allogeneic lymphoblastoid cell lines.
Misko
I S
IS
Queensland Institute of Medical Research, Brisbane, Australia.
Burrows
S R
SR
Schmidt
C
C
Bishop
C J
CJ
Ryan
J M
JM
Staples
J A
JA
Moss
D J
DJ
D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000302:isolation & purification; D003602:Cytotoxicity, Immunologic; D019309:Epstein-Barr Virus Nuclear Antigens; D006801:Humans; D066298:In Vitro Techniques; D007244:Infectious Mononucleosis / Q000276:immunology*; D007376:Interleukin-2 / Q000494:pharmacology*; D010641:Phenotype; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007376:Interleukin-2