The coat protein of beet curly top virus is essential for infectivity.
We have applied the procedure of Agrobacterium-mediated inoculation to develop a simple, efficient, and reproducible assay for the infectivity of the leafhopper-transmitted geminivirus, beet curly top virus (BCTV). This assay system was used to show that a coat protein mutant of BCTV is not infectious, but could be complemented by coagroinoculation with a second mutant bearing a lethal mutation in the complementary-sense open reading frame, C1. Furthermore, the coat protein mutant retained the ability to replicate and to produce both ssDNA and dsDNA when electroporated into Nicotiana tabacum protoplasts. We conclude that the coat protein of BCTV is essential for spread of the virus. The results are discussed in the light of results with coat protein mutants of other geminiviruses.
Briddon
R W
RW
Department of Virus Research, John Innes Institute, AFRC Institute of Plant Science Research, Norwich, United Kingdom.
Watts
J
J
Markham
P G
PG
Stanley
J
J
D015139:Blotting, Southern; D002213:Capsid / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D009154:Mutation; D010942:Plant Viruses / Q000235:genetics / Q000502:physiology*; D010947:Plants, Toxic; D011523:Protoplasts; D015183:Restriction Mapping; D014026:Nicotiana; D014162:Transfection
D004277:DNA, Single-Stranded; D004279:DNA, Viral