Changes in hypothalamic monoamine oxidase activity in relation to season, ovariectomy, and 17 beta-estradiol administration in intact and ovariectomized catfish, Clarias batrachus (L.).
Cyclic changes in hypothalamic monoamine oxidase (MAO) activity were noted during the annual ovarian cycle of Clarias batrachus, with a high level in the preparatory phase and a low level in the spawning phase. Administration of 17 beta-estradiol (E2) daily for 3 days induced both season- and dose-dependent responses in enzyme activity. In the preparatory phase, 0.05, 0.1, and 0.5 microgram/g doses of E2 enhanced enzyme activity in a dose-dependent manner, with significant changes being observed in the latter two dose groups. Administration of 1.0 and 10.0 micrograms/g doses decreased enzyme activity, with a significant difference elicited by the latter dose. In the spawning phase, enzyme activity was significantly elevated by 0.05 and 0.1 microgram/g doses. MAO activity decreased insignificantly in the 0.5 and 1.0 microgram/g groups and significantly in the 10.0 micrograms/g E2 group. Enzyme activity decreased significantly 2, 3, and 4 weeks after ovariectomy (prespawning phase), in a time-dependent manner, and increased after 5 weeks (not significantly different from the sham control value) and 6 weeks. Administration of E2 to 3-week ovariectomized fish elicited dose-dependent effects on MAO activity. E2 injections at 0.05 and 0.1 microgram/g doses not only restored the ovariectomy-induced decrease, but also elevated enzyme activity significantly compared with the sham + vehicle control. The higher doses (0.5 and 1.0 microgram/g) were ineffective in restoring enzyme activity, which decreased significantly in the 1.0 microgram/g group compared with ovariectomy and sham control values.(ABSTRACT TRUNCATED AT 250 WORDS)
Manickam
P
P
Department of Zoology, Banaras Hindu University, Varanasi, India.
Joy
K P
KP
D000818:Animals; D002397:Catfishes / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007031:Hypothalamus / Q000502:physiology*; D008995:Monoamine Oxidase / Q000378:metabolism*; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D012621:Seasons
D004958:Estradiol; D008995:Monoamine Oxidase