Antigenic expression of B-cell chronic lymphocytic leukemic lymphocytes.
A flow cytometric analysis of lymphocytes in B-cell chronic lymphocytic leukemia (B-CLL) samples and in monocyte-depleted and T-cell-depleted normal peripheral blood (B-PBL) samples was undertaken using 129 reagents from the blind panel (BP) and 72 reagents from the cluster designation (CD) panel obtained from the Fourth International Leucocyte Differentiation Conference and Workshop, B-Cell Section. After determining the average mean channel fluorescence and the average percentage of positive cells for the B-CLL and the normal B-PBL preparations, a combined ratio and difference analysis was performed for each monoclonal antibody reactivity. This analysis confirmed the intense expression of class II antigens on B-CLL and the preferential expression of CD19, CD20, CD23 and CD24 antigens. In addition, three new clustered and three new unclustered antigens were also preferentially expressed on B-CLL lymphocytes. Cluster analysis of these differences suggests the existence of at least three overlapping immunophenotypic subpopulations, composed of CD19, CD20, CD21, CD22, CD23, CD24, CD75, CD76 and CDw78.
Marti
G E
GE
Food and Drug Administration (FDA), Bethesda, Maryland.
Zenger
V
V
Caproaso
N E
NE
Brown
M
M
Washington
G C
GC
Carter
P
P
Schechter
G
G
Noguchi
P
P
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D001402:B-Lymphocytes / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D010641:Phenotype
D015703:Antigens, CD; D000951:Antigens, Neoplasm