Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3.
A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12,000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5'- and a longer 3'-noncoding region. The deduced protein sequence was verified by partial peptide sequencing.
Kriechbaum
M
M
Institut für Biochemie, Technische Hochschule Darmstadt, FRG.
Heilmann
H J
HJ
Wientjes
F J
FJ
Hahn
M
M
Jany
K D
KD
Gassen
H G
HG
Sharif
F
F
Alaeddinoglu
G
G
D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005800:Genes, Fungal; D005949:Glucose Oxidase / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis
D009841:Oligonucleotides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D004247:DNA; D005949:Glucose Oxidase