title: Cell proliferation and differentiation in the fetal and early postnatal mouse thymus.
abstract: The relationships between cell proliferation and cell differentiation during thymus ontogeny were studied by labeling DNA-synthesizing thymocytes with bromodeoxyuridine and staining with antibodies against CD4, CD8, J11d, phagocytic glycoprotein 1, TCR V beta 8 chain, Thy-1, and IL-2R surface proteins. The development of the thymus was discontinuous, with two well defined growth periods from 13 days to 18 days of fetal life and from 3 days to 6 days after birth, and more progressive growth from day 8 to 2 wk. Cell proliferation started on fetal day 12, 1 day after the arrival of hemopoietic stem cells in the third branchial pouch. These cells were phagocytic glycoprotein 1-positive but IL-2R and Thy-1 negative. Thus, cell proliferation preceded IL-2R expression. Until day 15, CD4-8- thymocytes expanded without differentiation. Then CD4-8+ and CD4+8+ cells appeared; this induction was proliferation dependent and occurred on cells which had already lost IL-2R, but just after maximum expression of this receptor. During several days, the thymus remained of constant size (around 10(7) cells) and behaved like the steady state thymus. On day 3 after birth, expansion started again and was correlated with an increase in CD4-8- proliferation index and IL-2R expression. At the same time, the thymic subset capable of expansion without differentiation was again, transiently, detectable. These results suggest that the inflow of precursor cells into the thymus is permanent but transiently increased at several times during ontogeny. Moreover, the behavior of fetal CD4-8- cells does not appear radically different from that of adult precursors, but the actual difference resides in the variation of the relative proportion of CD4-8- cells at different maturation stages, as revealed by striking variations of IL-2R expression by cycling cells.
authors: INSERM U 25-CNRS UA 122, Hôpital Necker, Paris, France.
authors: C
authors: C
authors: Penit
authors: F
authors: F
authors: Vasseur
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology* / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte; D002453:Cell Cycle; D002454:Cell Differentiation; D002470:Cell Survival; D005314:Embryonic and Fetal Development; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000502:physiology; D013950:Thymus Gland / Q000254:growth & development / Q000276:immunology / Q000502:physiology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte