B-lymphocytic cell line Raji expresses the leukotriene A4 hydrolase gene but not the 5-lipoxygenase gene.
The expression of LTA hydrolase and 5-lipoxygenase genes was studied in Raji cells, a Burkitt lymphoma derived B-cell line. Northern and Western blot analyses clearly showed the expression, both at the transcriptional and translational level, of the LTA4 hydrolase gene in these cells. However, expression of the 5-lipoxygenase gene was undetectable. Thus, the genes coding for the two enzymes required for biosynthesis of leukotriene B4 from arachidonic acid, 5-lipoxygenase and LTA4 hydrolase, were differentially expressed in the Raji cells.
Medina
J F
JF
Department of Physiological Chemistry, Karolinska Institute, Stockholm, Sweden.
Odlander
B
B
Funk
C D
CD
Fu
J Y
JY
Claesson
H E
HE
Rådmark
O
O
D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics*; D001091:Arachidonate Lipoxygenases / Q000235:genetics*; D001402:B-Lymphocytes / Q000201:enzymology*; D015152:Blotting, Northern; D015153:Blotting, Western; D002051:Burkitt Lymphoma; D004851:Epoxide Hydrolases / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D001091:Arachidonate Lipoxygenases; D001094:Arachidonate 5-Lipoxygenase; D004851:Epoxide Hydrolases; C043134:leukotriene A4 hydrolase