title: Contact-guidance of neurite growth: a direct or an indirect effect of physical environment?
abstract: Experiments with dissociated chick dorsal root ganglion cells cultured in different media revealed the significance of non-neuronal cells for neurite growth along a physical boundary. The evidence presented suggests that fibrillary fibronectin deposited by non-neuronal cells guides neurites, rather than the physical boundary itself. This guidance is not a consequence of preferential neurite growth on fibronectin deposits. Apparently fibronectin exerts different effects when present in a homogeneous vs. a fibrillary form. It is concluded, that these mechanisms account for the fact that elongating nerve fibers extend parallel to scratches in the culture dish surface and maintain a distance to it, when co-cultured with non-neuronal cells.
authors: Department of Anatomy, University of Goettingen, FRG.
authors: A
authors: A
authors: Michler
authors: D L
authors: DL
authors: Meyer
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D005347:Fibroblasts / Q000502:physiology; D005353:Fibronectins / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology; D007150:Immunohistochemistry
chemical_list: D003470:Culture Media; D005353:Fibronectins