An enzymatic method for oxalate automated with the Cobas Fara centrifugal analyzer.
Measurement of oxalate in urine has been automated for use with the Cobas Fara centrifugal analyzer. No sample pretreatment other than (a critical) pH adjustment is required. Between-run CVs were less than 4%. Results were linearly related to oxalate concentration to 1000 mumol/L. Ascorbic acid ingestion, up to 5 g of ascorbate daily, caused no demonstrable interference with the assay. This practical, automated method for assaying urinary oxalate is substantially faster than other chemical or enzymatic methods.
Allen
L C
LC
Department of Clinical Biochemistry, Toronto General Hospital, Ontario, Canada.
Kadijevic
L
L
Romaschin
A D
AD
D000445:Aldehyde Oxidoreductases; D001322:Autoanalysis / Q000295:instrumentation; D002262:Carboxy-Lyases; D002498:Centrifugation / Q000295:instrumentation; D005560:Formate Dehydrogenases; D006801:Humans; D010070:Oxalates / Q000592:standards / Q000652:urine*; D011933:Reagent Kits, Diagnostic
D010070:Oxalates; D011933:Reagent Kits, Diagnostic; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases; D002262:Carboxy-Lyases; C021915:oxalate decarboxylase