In vitro distribution of diacetyl didemnin B in human blood cells and plasma.
We have utilized [3H]diacetyl-didemnin B ([3H]DB) in a series of in vitro experiments to determine the time course of equilibration of [3H]DB between plasma and blood cells, the distribution of [3H]DB among blood cells, and the distribution of [3H]DB in plasma. Within 45-60 min at 37 degrees C, approximately 55% of [3H]DB added to whole blood remained in the plasma. Of the drug associated with blood cells, 77% was found with the red blood cells, 18% with the lymphocytes, and 5% with the granulocytes. However, on the basis of amount of drug uptake per cell, lymphocytes incorporated nearly 400 times more and granulocytes nearly 30 times more [3H]DB than red blood cells. No specific association of [3H]DB with a particular lymphocyte subset was detected. Additionally, it appears that [3H]DB in plasma was associated to a large extent with albumin.
Phillips
J L
JL
Cancer Therapy and Research Center, University of Texas Health Science Center, San Antonio.
Schwartz
R
R
Von Hoff
D D
DD
D000970:Antineoplastic Agents / Q000378:metabolism*; D001773:Blood Cells / Q000378:metabolism*; D047630:Depsipeptides; D004912:Erythrocytes / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism; D010456:Peptides, Cyclic / Q000378:metabolism* / Q000493:pharmacokinetics; D010949:Plasma / Q000378:metabolism*; D013997:Time Factors
D000970:Antineoplastic Agents; D047630:Depsipeptides; D010456:Peptides, Cyclic; C030051:didemnins