title: The cold-induced denaturation of lactate dehydrogenase at sub-zero temperatures in the absence of perturbants.
abstract: The cold-induced denaturation of lactate dehydrogenase has been determined in an unfrozen, cryoprotectant free solution at sub-zero temperatures. The cold-induced denaturation temperature (TL) has been found to be -28 degrees C. The results for the first time clearly establish that temperature alone can induce denaturation in a cooled protein solution. The validity of earlier data, obtained in the presence of perturbants (particularly pH or guanidinium chloride), is discussed.
authors: Pafra Ltd, Biopreservation Division, Cambridge, England.
authors: R H
authors: RH
authors: Hatley
authors: F
authors: F
authors: Franks
mesh_terms: D000818:Animals; D003080:Cold Temperature; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology; D011489:Protein Denaturation; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D007770:L-Lactate Dehydrogenase