Human monoclonal anti-idiotypic antibody to the tumour-associated antibody 791T/36.
A human monoclonal antibody, 105AD7, was produced by fusion of a mouse/human heteromyeloma cell line with lymphocytes from a patient previously injected with mouse monoclonal antibody 791T/36 for tumour immunoscintigraphy. The 105AD7 hybridoma has been in continuous culture for more than 12 months, producing a human monoclonal IgG1 which binds to 791T/36 (IgG2b) and its IgG2a class switch variant, but not a range of other monoclonal mouse immunoglobulins. In quantitative flow cytometric assays, 105AD7 was shown to block the binding of fluorescein-labelled 791T/36 to its target gp72 antigen at the surface of tumour cells, but not the binding of 228, an anti-carcinoembryonic antigen (CEA) monoclonal antibody to CEA. Tests with purified 105AD7 antibody demonstrated a stoichiometric high-affinity interaction between 105AD7 and 791T/36. Thus 105AD7 is a human anti-idiotypic antibody which binds at or very close to the binding site of 791T/36, and as such is a candidate for anti-idiotypic immunization of cancer patients.
Austin
E B
EB
Cancer Research Campaign Laboratories, University of Nottingham, U.K.
Robins
R A
RA
Durrant
L G
LG
Price
M R
MR
Baldwin
R W
RW
D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000918:Antibody Specificity; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007130:Immunoglobulin Idiotypes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes