[In vitro transformation of Syrian hamster embryo cells by four chemicals].
In this paper, the early passage diploid Syrian hamster embryo (SHE) cells were used as the source of target. Four chemicals were appraised in SHE transformation test to determine whether they were carcinogens or not. They were (1) 2-benzoyl-hydrazono-1,3-diethiolane(BHD) (technical product); (2) isoprothiolane (pure product); (3) isoprothiolane (technical product); (4) benzene-abstracts from coal smoke of coke oven (benzene-abstracts). The results showed that morphological transformation was not observed when cells were not treated or treated with dimethyl sulfoxide, BHD, pure isoprothiolane and the technical product of isoprothiolane. The highest concentration had considerable cytotoxicity. In the groups of positive control (1.0 microgram/ml 3-methyl-cholanthrene, 10.0 micrograms/ml benzo (a) pyrene) and benzene-abstracts, we could observe colonies with random or criss-cross orientation and dense piling-up of cells. According to well known positive criteria, the benzene-abstracts can induce SHE cells morphological transformation. The other three chemicals can not induce SHE cells transformation.
Zhang
Z Z
ZZ
Zeng
X G
XG
Guo
J T
JT
Sun
M L
ML
Zhang
X L
XL
D000818:Animals; D001554:Benzene / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D003031:Coal; D006224:Cricetinae; D004622:Embryo, Mammalian; D005659:Fungicides, Industrial / Q000633:toxicity*; D006835:Hydrazones / Q000633:toxicity*; D008647:Mesocricetus; D012906:Smoke / Q000032:analysis*; D013876:Thiophenes / Q000633:toxicity*
D003031:Coal; D005659:Fungicides, Industrial; D006835:Hydrazones; D012906:Smoke; D013876:Thiophenes; C049780:2-benzoylhydrazono-1,3-dithiolane; C023889:isoprothiolane; D001554:Benzene