Anti-idiotype antibody reactive with a target molecule for mouse lymphokine-activated killer cells.
A rat monoclonal antibody (MoAb), termed KBA, against mouse lymphokine-activated killer (LAK) cells recognizes a LAK cell surface molecule termed LAA responsible for the binding between LAK and target cells. In order to identify a target molecule of LAK cells, we prepared anti-KBA idiotype antibodies (anti-KBA-Id) from rabbit anti-KBA sera. Immunoglobulins were separated by ammonium sulfate precipitation followed by sequential affinity column chromatographies using Affi-gel coupled with rat MoAbs other than KBA and KBA-coupled gel. An immunoglobulin(s) in a KBA-gel-bound fraction showed the selective reactivity to KBA, comprising anti-KBA-Id character. This anti-KBA-Id inhibited the binding of KBA to LAK. Moreover, it bound with a portion of mouse leukemia cells sensitive to LAK cells, but not with normal mouse cells, and inhibited the binding of LAK cells to a target leukemia. These findings indicate that the anti-KBA-Id contain anti-Id which possess a three-dimensional structure that mimics a mirror image of the antigen (LAA)-combining site in KBA or the structure of LAA. The antigen reactive with anti-KBA-Id was characterized as a glycoprotein.
Sato
N
N
Department of Hygienic Chemistry, Tohoku University, Sendai, Japan.
Masuko
T
T
Nishimura
T
T
Kato
K
K
Hashimoto
Y
Y
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007130:Immunoglobulin Idiotypes; D008222:Lymphokines