The physical state of the erythrocyte membrane in myotonic dystrophy.
The molecular pathology of myotonic dystrophy is believed to be expressed at the plasma membrane level. Previous assessments of membrane fluidity, a marker of the biochemical state of the membrane, have yielded conflicting results. In this study, erythrocyte membrane fluidity was reevaluated using highly sensitive fluorescence probe techniques. Steady-state anisotropy was measured with diphenylhexatriene (DPH), trimethylaminophenyl-hexatriene (TMA-DPH) and phenylhexatrienylphenylpropionic acid, probing different regions of the membrane. In the patients, significantly increased steady-state anisotropy was obtained with DPH, probing the hydrophobic core of the membrane, while slightly reduced anisotropy was found with TMA-DPH. The dynamic properties of the membrane lipids were further examined by means of time-resolved measurements with DPH. The excited state decay kinetics could best be described by a bi-exponential decay model. A large redistribution of the probe populations and a reduction of the average order parameter were found in the patients indicating a less ordered or more fluid lipid matrix. These perturbations might be induced by a protein abnormality and altered protein-lipid interaction within the erythrocyte membrane.
Beaugé
F
F
Unité de Neurotoxicologie, INSERM U.26, Hôpital F. Widal, Paris, France.
Gallay
J
J
Sydow
O
O
Stibler
H
H
D000293:Adolescent; D000328:Adult; D004912:Erythrocytes / Q000473:pathology*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008560:Membrane Fluidity; D008875:Middle Aged; D009136:Muscular Dystrophies / Q000097:blood*
D005456:Fluorescent Dyes