title: Developmental coupling of expression of the Igh-linked minor antigen H-40 to membrane immunoglobulin expression.
abstract: C.B-20 (Ighb) but not BALB/c (Igha) mice are able to reject BCL1, a spontaneous B cell leukemia of BALB/c origin. The protective immune response is directed toward the minor histocompatibility (H) antigen, H-40, which can be detected by cytotoxic T lymphocytes and is expressed on surface (s) immunoglobulin positive tumor cells including BCL1 as well as sIg+ lymphoblasts activated by lipopolysaccharide. The fact that only sIg+ tumor cells and lymphoblasts express H-40 suggests that sIg itself may be a component involved in CTL recognition of this antigen. However, this possibility was ruled out by demonstrating that removal of sIg from target cells did not prevent H-40-specific CTL recognition. To determine whether H-40 expression was coordinately regulated with that of membrane Ig, we determined whether H-40- sIg- cells acquire H-40 when induced to express sIg. We also transfected a functional mu c and nu region gene into a sIg- lymphoma so that it acquired a sIg+ phenotype. In neither case did such sIg+ cells acquire H-40. Together, these data indicate that H-40 is expressed on B cells representing a particular stage of differentiation that is coincidental with sIg. We previously showed that H-40 and Igh loci are linked on the 12th murine chromosome. In this study we further localized H-40 to a point beyond Aat (formerly Pre-1) near Lm-1, a locus previously described to encode minor H-antigens expressed on lymphomyeloid cells. Thus, H-40 and Lm-1 may represent a gene cluster encoding antigens expressed on subsets of lymphoid and myeloid cells.
authors: Immunology Graduate Program, University of Texas Southwestern Medical Center, Dallas 75235-9048.
authors: K L
authors: KL
authors: Schreiber
authors: C
authors: C
authors: Webb
authors: P
authors: P
authors: Tucker
authors: R
authors: R
authors: Riblet
authors: J
authors: J
authors: Forman
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D002874:Chromosome Mapping; D003602:Cytotoxicity, Immunologic; D005786:Gene Expression Regulation; D006183:H-2 Antigens / Q000276:immunology; D007075:Immunoglobulin M / Q000235:genetics / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008912:Minor Histocompatibility Loci; D011947:Receptors, Antigen, B-Cell / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
chemical_list: D006183:H-2 Antigens; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell