title: Detection of cell-affecting agents with a silicon biosensor.
abstract: Cellular metabolism is affected by many factors in a cell's environment. Given a sufficiently sensitive method for measuring cellular metabolic rates, it should be possible to detect a wide variety of chemical and physical stimuli. A biosensor has been constructed in which living cells are confined to a flow chamber in which a potentiometric sensor continually measures the rate of production of acidic metabolites. Exploratory studies demonstrate several applications of the device in basic science and technology.
authors: Molecular Devices Corporation, Menlo Park, CA 94025.
authors: J W
authors: JW
authors: Parce
authors: J C
authors: JC
authors: Owicki
authors: K M
authors: KM
authors: Kercso
authors: G B
authors: GB
authors: Sigal
authors: H G
authors: HG
authors: Wada
authors: V C
authors: VC
authors: Muir
authors: L J
authors: LJ
authors: Bousse
authors: K L
authors: KL
authors: Ross
authors: B I
authors: BI
authors: Sikic
authors: H M
authors: HM
authors: McConnell
mesh_terms: D015374:Biosensing Techniques; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002477:Cells / Q000378:metabolism*; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D010101:Oxygen Consumption; D012825:Silicon
chemical_list: D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D004815:Epidermal Growth Factor; D012825:Silicon