Entry of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine into the rat brain.
We studied blood-to-brain entry of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1-methyl-4-phenylpyridinium (MPP+) and butanol in anesthetized rats using the indicator-fractionation method with right atrial bolus injection. Minimal amounts of MPP+, which has low octanol/water partition coefficient, crossed the blood-brain barrier. MPTP and butanol, both of which have high octanol/water partition coefficients, were almost completely extracted by all regions of the brain on the first pass. The main difference between the MPTP and butanol tracers is that butanol rapidly left the brain with an exponential rate constant of 1.24 min-1, whereas MPTP was avidly retained by the brain with a washout rate constant of 0.10 min-1 (mean values for the four brain regions that we studied). Early retention of MPTP by the brain was not due to its rapid metabolism by monoamine oxidase because pargyline pretreatment did not affect this rate constant. However, 30 min after [3H]MPTP injection, brain retention of the 3H tracer was reduced significantly by pargyline treatment, and the ratio of brain MPTP/MPP+ was increased markedly.
Riachi
N J
NJ
Department of Neurology, University Hospital of Cleveland, Ohio.
LaManna
J C
JC
Harik
S I
SI
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015655:1-Methyl-4-phenylpyridinium; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D000440:Butanols / Q000493:pharmacokinetics; D008297:Male; D010293:Pargyline / Q000494:pharmacology; D011725:Pyridines / Q000493:pharmacokinetics*; D011726:Pyridinium Compounds / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D000440:Butanols; D011725:Pyridines; D011726:Pyridinium Compounds; D014316:Tritium; D010293:Pargyline; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015655:1-Methyl-4-phenylpyridinium