Involvement of transforming growth factor-alpha secreted by macrophages in metallothionein induction by endotoxin.
The mechanism of metallothionein (MT) induction of the liver by endotoxin, which is mediated by a factor secreted by endotoxin-stimulated macrophages, was studied in vitro. MT induction of the liver cells by the endotoxin-stimulated macrophage conditioned medium was inhibited by a monoclonal antiepidermal growth factor (EGF) / transforming growth factor-alpha (TGF-alpha) receptor antibody, which acts as an antagonist of EGF and TGF-alpha. MT was induced by the substance, which was adsorbed by polyclonal antibody to TGF-alpha, but not by a monoclonal antibody to EGF, in the conditioned medium of endotoxin-stimulated macrophages. These results suggest that TGF-alpha secreted by macrophages is involved in MT induction by endotoxin.
Iijima
Y
Y
Department of Anesthesiology and Resuscitology Okayama University Medical School, Japan.
Fukushima
T
T
Kosaka
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000494:pharmacology*; D004790:Enzyme Induction; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology; D008264:Macrophages / Q000378:metabolism*; D008668:Metallothionein / Q000096:biosynthesis*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D015291:Transforming Growth Factors / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D004731:Endotoxins; D008070:Lipopolysaccharides; C044101:endotoxin, Escherichia coli; D015291:Transforming Growth Factors; D008668:Metallothionein