Evidence of potential tumour-initiating and tumour-promoting activities of hickory smoke condensate when given alone or with nitrite to rats.
Epidemiological studies have indicated that the consumption of smoked fish and meat products is associated with an increased risk of stomach cancer. A commercial hickory smoke condensate (HSC) was evaluated for its tumour-initiating and promoting activities in the glandular stomach using short-term methods in vivo. HSC (1 ml of a 10-100% v/v solution/rat) was given orally, either with or without nitrite (25-100 mumols/rat) to male F344 rats. The potential of HSC to act as a glandular stomach carcinogen was revealed by the induction in the pyloric mucosa of the stomach of ornithine decarboxylase (ODC), replicative DNA synthesis (RDS) and DNA single-strand breaks. The administration of HSC with nitrite also induced unscheduled DNA synthesis in the pyloric mucosa, but led to decreased induction of ODC, RDS and DNA single-strand breaks in comparison with treatment with HSC alone. These results suggest that HSC contains substance(s) that have potential tumour-initiating and/or tumour-promoting activities and that reaction with nitrite generates new substance(s) that could act as potential tumour-initiators in the rat glandular stomach.
Ohshima
H
H
Unit of Environmental Carcinogens and Host Factors, International Agency for Research on Cancer, Lyon, France.
Furihata
C
C
Matsushima
T
T
Bartsch
H
H
D000818:Animals; D002273:Carcinogens; D003043:Cocarcinogenesis; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D005511:Food Handling; D005753:Gastric Mucosa / Q000201:enzymology; D008297:Male; D009573:Nitrites / Q000633:toxicity*; D009955:Ornithine Decarboxylase / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012906:Smoke; D013274:Stomach Neoplasms / Q000139:chemically induced; D014934:Wood
D002273:Carcinogens; D009573:Nitrites; D012906:Smoke; D009955:Ornithine Decarboxylase