title: Chronobiological study of several enzymes of lysosomal origin in human plasma.
abstract: The possible occurrence of circadian and circannual rhythms in the plasma concentrations of the following enzymes of lysosomal origin was assessed: beta-D-N-acetylglucosaminidase (EC 3.2.1.30) beta-D-glucuronidase (EC 3.2.1.31), beta-D-glucosidase (EC 3.2.1.21), beta-D-galactosidase (EC 3.2.1.22), alpha-D-galactosidase (EC 3.2.1.23), alpha-L-fucosidase (EC 3.2.1) and alpha-D-mannosidase (EC 3.2.1.24). The circadian rhythm was studied in 16 women (aged: 17-24 years) and 13 men (age: 23 years) volunteers; the circannual rhythm, in 10 women and 8 men (age: 20-25 years). The circadian rhythm was detected in all the tested enzymes of women, and only in alpha-D-galactosidase, beta-D-glucosidase, alpha-D-mannosidase and beta-D-acetylglucosaminidase of men. A statistically significant difference between genders in the circadian rhythm was exhibited by beta-D-galactosidase (MESOR; amplitude) beta-D-glucosidase (MESOR; amplitude; acrophase) beta-D-N-acetylglucosaminidase, beta-D-glucuronidase and alpha-D-galactosidase (MESOR) and alpha-L-fucosidase (amplitude, acrophase). A circannual rhythm was detected in all the tested enzymes with the exception of beta-D-glucuronidase and beta-D-N-acetylglucosaminidase; no statistically significant difference between genders was detected. The group rhythms of some of the enzymes (alpha-D-galactosidase, beta-D-glucosidase, beta-D-galactosidase) showed similar values of both circadian and circannual acrophases, suggesting that they may subjected as a group to the same chronobiological coordination, possibly mediated by hormones. The chronobiological rhythms of lysosomal enzymes were different from those of lactate dehydrogenase and alpha 1-antitrypsin, indicating that these rhythms are not merely reflecting fluctuations of the water content of plasma. No in-phase relationship was observed between the circadian and circannual rhythms of plasma cortisol and those of the tested lysosomal enzymes, excluding a direct chronobiological and possibly functional relationship between this hormone and lysosomal enzymes.
authors: Dipartimento di Chimica e Biochimica Medica, Facoltà di Medicina, Università degli Studi di Milano, Italy.
authors: G
authors: G
authors: Goi
authors: A
authors: A
authors: Fabi
authors: A
authors: A
authors: Lombardo
authors: L
authors: L
authors: Caimi
authors: G
authors: G
authors: Tettamanti
authors: N
authors: N
authors: Montalbetti
authors: M
authors: M
authors: Cavalleri
authors: F
authors: F
authors: Halberg
mesh_terms: D000328:Adult; D001831:Body Temperature; D002940:Circadian Rhythm; D005260:Female; D006026:Glycoside Hydrolases / Q000097:blood*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D010507:Periodicity; D012016:Reference Values; D012737:Sex Factors; D000515:alpha 1-Antitrypsin / Q000032:analysis
chemical_list: D000515:alpha 1-Antitrypsin; D007770:L-Lactate Dehydrogenase; D006026:Glycoside Hydrolases; D006854:Hydrocortisone