Distribution of oleanolic acid glycosides in vacuoles and cell walls isolated from protoplasts and cells of Calendula officinalis leaves.
The contents of oleanolic acid and its 3-0-glucuronide derivatives as well as of 3-0-glucoside derivatives were determined in vacuoles prepared from protoplasts and cell walls obtained from cells of Calendula officinalis leaves. In both cell compartments studied 37% of total cellular oleanolic acid were accumulated, 0.6% occurring as free oleanolic acid (only in vacuoles). Glucuronides accounted for 31.1% (20.7% in vacuoles and 10.4% in cell walls), and glucosides for 5.3% (2.6% in vacuoles and 2.7% in cell walls).
Szakiel
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Institute of Biochemistry, University of Warsaw, Poland.
Kasprzyk
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D002473:Cell Wall / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D006056:Golgi Apparatus / Q000032:analysis; D007425:Intracellular Membranes / Q000032:analysis; D009828:Oleanolic Acid / Q000032:analysis; D010944:Plants / Q000648:ultrastructure*; D011523:Protoplasts / Q000032:analysis*; D014617:Vacuoles / Q000032:analysis*
D006027:Glycosides; D009828:Oleanolic Acid