Neutron irradiation of human melanoma cells.
The biological characteristics and in vitro radiosensitivity of melanoma cells to thermal neutrons were investigated as a guide to the effectiveness of boron neutron capture therapy. Plateau phase cultures of three human malignant melanoma-established cell lines were examined for cell density at confluence, doubling time, cell cycle parameters, chromosome constitution, and melanin content. Cell survival dose-response curves, for cells preincubated in the presence or absence of p-boronophenylalanine. HCl (10B1-BPA), were measured over the dose range 0.6-8.0 Gy (N + gamma). The neutron fluence rate was 2.6 x 10(9) n/cm2/s and the total dose rate 3.7 Gy/h (31% gamma). Considerable differences were observed in the morphology and cellular properties of the cell lines. Two cell lines (96E and 96L) were amelanotic, and one was melanotic (418). An enhanced killing for neutron irradiation was found only for the melanotic cells after 20 h preincubation with 10 micrograms/ml 10B1-BPA. In view of the doubling times of the cell lines of about 23 h (96E and 96L) or of 36 h (418), it seems likely that an increased boron uptake, and hence increased radiosensitivity, might result if the preincubation period with 10B1-BPA is extended to several hours longer than the respective cell cycle times.
Brown
K
K
Australian Nuclear Science and Technology Organisation, Lucas Heights Research Laboratory, Menai, New South Wales.
Mountford
M H
MH
Allen
B J
BJ
Mishima
Y
Y
Ichihashi
M
M
Parsons
P
P
D001896:Boron Compounds / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use / Q000633:toxicity*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007554:Isotopes; D008545:Melanoma / Q000473:pathology / Q000532:radiotherapy*; D009502:Neutrons; D010649:Phenylalanine / Q000008:administration & dosage / Q000627:therapeutic use / Q000633:toxicity / Q000031:analogs & derivatives; D012878:Skin Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000528:radiation effects
D001896:Boron Compounds; D007554:Isotopes; D010649:Phenylalanine; C033685:4-boronophenylalanine