Effects of epidermal growth factor, fibroblast growth factor, retinoic acid and serum on anchorage-dependent and anchorage-independent growth of HRRT cells.
The effects of EGF, FGF, RA and serum on anchorage-dependent and anchorage-independent growth of HRRT cells were studied. The five different types of serum tested in the present work induced a dose dependent rise in anchorage-independent growth in aggregates. FCS, SBCS and RS also supported colony formation in soft agar, whereas BS and HS had no significant effect. EGF and FGF stimulated anchorage-dependent growth of HRRT cells in monolayers. The peptide growth factors were also found to induce phenotypic transformation of the nonneoplastic HRRT cells, as measured by anchorage-independent growth in soft agar as well as in aggregates. At equimolar concentrations EGF was much more effective than FGF. The stimulating effect of EGF and FGF on cell proliferation in the aggregate form was markedly inhibited by RA. Treatment of HRRT cells with the highest noncytotoxic concentration of RA, 2 x 10(-7) M, reduced the stimulating effect of both growth factors by about 60%.
Eker
P
P
Department of Biochemistry, Norwegian Radium Hospital, Oslo.
D001769:Blood; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000166:cytology*; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor