A mating-type-specific sterility gene map1 is required for transcription of a mating-type gene mat1-Pi in the fission yeast Schizosaccharomyces pombe.
Transcriptional activation of the mating-type gene mat1-Pi essential for meiosis in the fission yeast Schizosaccharomyces pombe was studied. A homozygous diploid harboring the mutation at the h+-specific sterility gene, map1, was arrested before premeiotic DNA synthesis in the nitrogen-free sporulation medium. Transcription of mat1-Pi was totally absent in the map1 mutant. The mei2 gene encoding a positive regulator for meiosis was normally transcribed in the map1 mutant, suggesting that the map1 function was specific to mat1-Pi.
Fujioka
H
H
Department of Biology, Faculty of Science, Osaka City University, Japan.
Shimoda
C
C
D015152:Blotting, Northern; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D008540:Meiosis; D009154:Mutation; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D014158:Transcription, Genetic
D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger