Induction of murine hemopoietic growth factors by toxic shock syndrome toxin-1.
Toxic shock syndrome toxin-1 (TSST-1), an extra-cellular 22 kDa single chain protein produced by most Staphylococcus aureus strains isolated from patients with toxic shock syndrome (TSS), induces modifications of blood cell values similar to those observed during TSS. We therefore analyzed the effects of TSST-1 on the proliferation and differentiation of murine granulocyte-macrophage progenitor cells (CFU-culture) and the eventual role of endotoxin in this response. TSST-1 had no direct effect on the proliferation of CFU-culture and was unable to influence the CSF-induced proliferation and differentiation of these progenitors. In contrast, TSST-1 was a potent inducer in spleen cell cultures of a factor with an ability to induce both colony formation by bone marrow cells and proliferation of an IL-3-dependent cell line. Nanogram amounts of TSST-1 were able to induce the release of CSF activity in spleen cell cultures from both normal and LPS-hyporesponsive mice. Cells from C3H/HeJ mice were as responsive as cells from C3H/He Pas mice. Furthermore, in spleen cell cultures from normal mice, TSST-1 and LPS did not act synergistically to induce CSF activity. Nanogram amounts of TSST-1 were also able to induce CSF activity in vivo but failed to induce IL-3 activity in the serum and organ-conditioned media from TSST-1-treated mice.
Galelli
A
A
C.N.R.S. UA-359, Institut Pasteur, Paris, France.
Anderson
S
S
Charlot
B
B
Alouf
J E
JE
D000818:Animals; D001427:Bacterial Toxins; D002455:Cell Division / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000276:immunology / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D007375:Interleukin-1 / Q000096:biosynthesis; D007377:Interleukin-3 / Q000096:biosynthesis / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D011817:Rabbits; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D018089:Superantigens
D001427:Bacterial Toxins; D003115:Colony-Stimulating Factors; D004768:Enterotoxins; D007375:Interleukin-1; D007377:Interleukin-3; D008070:Lipopolysaccharides; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal