Gas chromatographic analysis of free and bound malonaldehyde in rat liver homogenates.
A gas chromatographic method for the measurement of free and bound malonaldehyde (MA) in rat liver homogenate was developed to investigate biological damage caused by lipid peroxidation. Free MA was derivatized 1-methylpyrazole (1-MP), which was subsequently analyzed by capillary gas chromatography (GC). Bound forms of MA were determined by first hydrolyzing samples in acetic acid, then measuring the free MA as 1-MP. Bound MA in rat liver was released within 15 min by acid hydrolysis. A slight increase in bound MA levels in rat liver was observed 1 hr after CCl4 treatment, with bound MA then returning to control levels. Free MA levels and thiobarbituric acid assay (TBA) values did not show appreciable changes over a 24-hr period after CCl4 administration. However, the TBA values were approximately ten times the MA values in most samples.
Ichinose
T
T
Department of Environmental Toxicology, University of California, Davis 95616.
Miller
M G
MG
Shibamoto
T
T
D000818:Animals; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002849:Chromatography, Gas; D006868:Hydrolysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism*; D008297:Male; D008314:Malonates; D008315:Malondialdehyde / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013858:Thiobarbiturates
D008314:Malonates; D013858:Thiobarbiturates; D002084:Butylated Hydroxytoluene; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; C029684:thiobarbituric acid