Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.
A mutant screen employing the ade6-M26 recombination hotspot was developed and used to isolate Schizosaccharomyces pombe mutants deficient in meiotic recombination. Nine rec mutations were recessive, defining six complementation groups, and reduced ade6 meiotic recombination 3-fold to greater than or equal to 300-fold when homozygous. Three recessive rec mutations analyzed further also reduced meiotic intragenic recombination at ura4 on chromosome III and intergenic recombination between pro2 and arg3 on chromosome I. The observed non-co-ordinate reductions of the recombinant frequencies in the three test intervals suggest a degree of locus (or intragenic vs. intergenic) specificity of the corresponding rec+ gene products. None of the mutations specifically inactivated the ade6-M26 hotspot. Additional rec genes may be identified with these methods.
Ponticelli
A S
AS
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Smith
G R
GR
D003433:Crosses, Genetic; D008540:Meiosis; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology; D010957:Plasmids; D011995:Recombination, Genetic; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D013170:Spores / Q000502:physiology; D014170:Transformation, Genetic
D009604:Nitrosoguanidines