title: Selective potentiation of lymphocyte-derived macrophage chemotactic factor release in complete Freund's adjuvant-treated guinea pigs.
abstract: Dinitrophenol (DNP)-ovalbumin(OA)-induced tissue macrophage reaction in sensitized guinea pigs is enhanced by treatment with complete Freund's adjuvant (CFA). The enhancement of the reaction may be due to the increased production of a T-lymphocyte-derived macrophage chemotactic factor (LDMCF) because treatment of animals with CFA potentiates antigen- and concanavalin A(ConA)-induced release of LDMCF activity from spleen cells of the CFA-treated animals in vitro. This potentiating effect by CFA seems to be ascribed to the release of an adherent-cell-derived soluble factor from the CFA-treated animals. The adherent cell-derived factor, LDMCF-potentiating factor (LDMCF-PF), preferentially potentiates the release of LDMCF activity but not of eosinophil chemotactic activity from antigen- or Con-A-stimulated T lymphocytes. Protein synthesis is required for release of LDMCF-PF. Molecular weight of LDMCF-PF activity is assumed to be about 10,000-20,000. LDMCF-PF activity is sensitive to trypsin, to neuraminidase, and also to alkalinity at pH 11, suggesting that LDMCF-PF is a glycoprotein. The present study provides one explanation for the enhanced macrophage reaction in delayed-type hypersensitivity reactions.
authors: Department of Pathology, Kumamoto University Medical School, Japan.
authors: K
authors: K
authors: Sakata
authors: K
authors: K
authors: Muramoto
authors: M
authors: M
authors: Hirashima
authors: H
authors: H
authors: Hayashi
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002630:Chemotactic Factors / Q000032:analysis / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006168:Guinea Pigs; D008264:Macrophages; D008297:Male; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D002630:Chemotactic Factors; D003470:Culture Media; D003208:Concanavalin A; D005620:Freund's Adjuvant