title: The sequence of the mouse 14 kDa beta-galactoside-binding lectin and evidence for its synthesis on free cytoplasmic ribosomes.
abstract: The partial amino acid sequence of the mouse 14 kDa beta-galactoside-binding lectin has been deduced from cDNA clones corresponding to 86% of the coding sequence and extending to the polyadenylation signal. The deduced amino acid sequence for the murine lectin shows 94% identity with the rat, 89% with human, 86% with bovine and 46% with the chicken 14 kDa lectins. A cDNA probe has been used to analyse genomic DNA and identify a single mRNA of approx. 570 bp in 3T3 fibroblasts, murine erythroleukaemia cells and the murine basement-membrane-secreting Engelbreth-Holm-Swarm tumour. Analysis of free and bound polyribosomes has shown that the lectin message is translated on free cytoplasmic ribosomes.
authors: AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
authors: T J
authors: TJ
authors: Wilson
authors: M N
authors: MN
authors: Firth
authors: J T
authors: JT
authors: Powell
authors: F L
authors: FL
authors: Harrison
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis*; D005697:Galactosides; D037161:Galectins; D006027:Glycosides; D006388:Hemagglutinins; D051379:Mice; D008969:Molecular Sequence Data; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D005697:Galactosides; D037161:Galectins; D006027:Glycosides; D006388:Hemagglutinins