title: Use of an autologous reaction in vitro to assess contributions of T and B lymphocytes to immune hyperreactivity of atopics.
abstract: The in-vitro proliferation reaction of peripheral blood lymphocytes (measured by [3H]thymidine incorporation) to autologous pokeweed mitogen (PWM)-induced lymphoblasts (PWM-lymphoblast-stimulated autologous mixed leucocyte reaction, PWM.AMLR) was used as a measure of immune hyperreactivity for comparison of atopic with non-atopic individuals. Accordingly, 10/24 non-atopics responded in the PWM.AMLR, and 19/19 atopics reacting to inhaled allergens responded. Autologous stimulation was associated with release of mitogenic factors from the PWM-activated stimulating cells (2/15 non-atopics, 9/15 atopics). For non-atopics, stimulation delivered by staphylococcus A (SAC)-activated cells was similar to that delivered by PWM-induced cells, while in atopics, the SAC.AMLR was never more than 50% of the PWM.AMLR, indicating a possible T cell component. Separation by panning of the stimulation cells into lymphocyte subsets supported the notion that stimulation involved a cooperation between B and T4+ T cells. It is proposed that a positive PWM.AMLR is dependent upon an initial B cell activation followed by the PWM stimulus dependent upon a previous T cell activation, where atopics have more lymphocytes in an activated state than healthy non-atopics. Such a baseline priming may contribute to an innate sensitivity of atopics to environmental allergens.
authors: Lautenberg Center for General and Tumor Immunology, Hebrew University, Hadassah Hospital Medical School, Jerusalem, Israel.
authors: J S
authors: JS
authors: Duke-Cohan
authors: R
authors: R
authors: Hirt
authors: M
authors: M
authors: Rottem
authors: A
authors: A
authors: Ben-Zvi
authors: A
authors: A
authors: Rubinow
authors: D
authors: D
authors: Naor
mesh_terms: D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D001430:Bacteriocins / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D011043:Pokeweed Mitogens / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D001430:Bacteriocins; D011043:Pokeweed Mitogens; C017724:staphylococcin