Prevention of transfusion-induced graft-versus-host disease in dogs by ultraviolet irradiation.
Ten dogs were given 9.2 Gy of total body irradiation and autologous bone marrow infusion followed by ten daily transfusions of leukocytes for a total of 11.5 to 36.2 (median, 18.8) x 10(8)/kg obtained via leukapheresis from histoincompatible unrelated donors. Four dogs were given unirradiated leukocytes, and all developed graft-versus-host disease (GVHD). In contrast, only two of three dogs given leukocytes irradiated with 20 mJ/cm2 of ultraviolet (UV) light (200 to 300 nm), and none of three dogs given leukocytes irradiated with 1,000 mJ/cm2 developed GVHD. These data indicate that UV irradiation abrogates the alloreactive potential of transfused leukocytes, and suggest that UV irradiation can be used to prevent the development of transfusion-induced GVHD.
Deeg
H J
HJ
Vincent T. Lombardi Cancer Research Center, Georgetown University, Washington, DC.
Graham
T C
TC
Gerhard-Miller
L
L
Appelbaum
F R
FR
Schuening
F
F
Storb
R
R
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D016026:Bone Marrow Transplantation / Q000276:immunology*; D004285:Dogs; D004307:Dose-Response Relationship, Radiation; D006086:Graft vs Host Disease / Q000517:prevention & control*; D006648:Histocompatibility; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D014466:Ultraviolet Rays
D015395:Histocompatibility Antigens Class I