Ontogeny of estrogen binding sites in the brain of gray short-tailed opossums (Monodelphis domestica).
This study evaluated the binding of [3H]estradiol to brain cytosols from gray short-tailed opossums ranging in age from newborn to 63 days postnatal. Estrogen binding was undetectable in whole-brain cytosol of newborn opossums. By postnatal day 4, high affinity (Kd = 0.2 nM), saturable (Bmax = 3 fmol/mg protein) estrogen binding sites were present, and estrogen binding in whole-brain remained low but detectable (2-5 fmol/mg) through day 63. In contrast, estrogen binding sites in the hypothalamus-preoptic area increased substantially from 3.4 fmol/mg on day 16 to 14 fmol/mg by day 63.
Etgen
A M
AM
Department of Psychiatry, Albert Einstein College of Medicine, Bronx, NY 10461.
Fadem
B H
BH
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D008297:Male; D009893:Opossums / Q000254:growth & development / Q000378:metabolism*; D011301:Preoptic Area / Q000254:growth & development / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism* / Q000502:physiology
D011960:Receptors, Estrogen; D004958:Estradiol