In vitro amplification of the alpha 1-antitrypsin gene: application to prenatal diagnosis.
Discrimination of the M, Z, and S alleles of alpha 1-antritrypsin (AAT) has been carried out using in vitro gene amplification with the polymerase chain reaction (PCR). Amplification of 90 nucleotides surrounding the Z mutation site and 120 nucleotides surrounding the S mutation site dramatically improves the sensitivity and reliability of allele-specific oligonucleotide (ASO) hybridization for direct detection of these alleles. Analysis is performed using Southern blots or dot blots hybridized with 19 base oligonucleotides and differentially washed for allele specificity. Amplification of the Z and S mutation sites can be combined in one PCR to allow detection of both mutations when analysed by gel electrophoresis and Southern transfer. This technique can be performed reliably using less than 0.1 micrograms of genomic DNA or less than 100 amniocytes or white blood cells. This technique has been used to perform prenatal diagnosis on a chorionic villus sample (CVS) in a fetus at risk for the ZZ Pi type form of AAT deficiency.
Hejtmancik
J F
JF
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas.
Holcomb
J D
JD
Howard
J
J
Vanderford
M
M
D000483:Alleles; D015139:Blotting, Southern; D004247:DNA; D005260:Female; D005784:Gene Amplification; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011296:Prenatal Diagnosis; D000515:alpha 1-Antitrypsin / Q000235:genetics*; D019896:alpha 1-Antitrypsin Deficiency
D000515:alpha 1-Antitrypsin; D004247:DNA