Permanent alteration of the murine Ly-1 B repertoire due to selective depletion of Ly-1 B cells in neonatal animals.
Studies presented here demonstrate that paternal allotype Ly-1 B cells are permanently depleted following neonatal treatment with antibodies to the paternal IgM allotype. Paternal allotype conventional B cells, in contrast, are temporarily depleted by treatment with either anti-IgM or anti-IgD allotype antibodies and return rapidly to normal frequencies once the antibody treatment disappears. These differences are explained by basic developmental differences between Ly-1 B and conventional lineage B cells. That is, the conventional B cell population is replenished from Ig- precursors throughout life and, therefore, is only temporarily affected when depleted in neonates. The Ly-1 B cell population, in contrast, develops from Ig- progenitors during the prenatal and neonatal life but survives because it is exclusively self-replenishing in adults. Therefore, elimination of a population of Ly-1 B cells from neonates is tantamount to removing it forever. These findings suggest that while conventional B cells turn over rapidly and have an effectively unlimited repertoire, Ly-1 B cells express a repertoire whose composition is strongly influenced by neonatal conditions that favor or select against the retention of cells producing certain antibody molecules. Thus, Ly-1 B cells play a unique role in the immune system in that they retain indefinitely the history of the neonatal animal's immunological experience.
Lalor
P A
PA
Department of Genetics, Stanford University, CA 94305.
Stall
A M
AM
Adams
S
S
Herzenberg
L A
LA
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000950:Antigens, Ly / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D004912:Erythrocytes / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology; D007126:Immunoglobulin Allotypes / Q000276:immunology*; D007072:Immunoglobulin D / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA
D000888:Antibodies, Anti-Idiotypic; D000950:Antigens, Ly; D007126:Immunoglobulin Allotypes; D007072:Immunoglobulin D; D007075:Immunoglobulin M