Chromosomal localization of the gene for the human trifunctional enzyme, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase.
A trifunctional protein in man, 5,10-methylenetetrahydrofolate dehydrogenase-5,10-methenyltetrahydrofolate cyclohydrolase-10-formyltetrahydrofolate synthetase, catalyzes three consecutive steps in the interconversion of tetrahydrofolate derivatives; these derivatives supply one-carbon units for intermediary metabolism. Somatic cell hybridization and in situ hybridization were used to localize the functional gene coding for this protein--to human chromosome 14q24, near the c-fos and TGF-beta 3 loci. A second hybridizing sequence, possibly a pseudogene, was identified near the centromere of the X chromosome, at Xp11.
Rozen
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Department of Pediatrics, McGill University-Montreal Children's Hospital Research Institute, Quebec, Canada.
Barton
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D
Du
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Hum
D W
DW
MacKenzie
R E
RE
Francke
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U
D000619:Aminohydrolases / Q000235:genetics*; D000818:Animals; D015139:Blotting, Southern; D002883:Chromosomes, Human, Pair 14; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D005260:Female; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D008025:Ligases / Q000235:genetics*; D008297:Male; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D009097:Multienzyme Complexes / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000235:genetics*; D015183:Restriction Mapping; D014960:X Chromosome
D015342:DNA Probes; D009097:Multienzyme Complexes; C021836:formyl-methenyl-methylenetetrahydrofolate synthetase; D004247:DNA; D010088:Oxidoreductases; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases; D008025:Ligases; D005574:Formate-Tetrahydrofolate Ligase