Ammonia and urea dynamics in the Lake Magadi tilapia, a ureotelic teleost fish adapted to an extremely alkaline environment.
The tilapia Oreochromis alcalicus grahami, which thrives under harshly alkaline conditions in Lake Magadi, Kenya, was studied in its natural environment (pH = 10, total CO2 = 180 mmol/L, osmolality = 525 mOsm/kg, 30-36.5 degrees C). At rest, this species excretes all nitrogenous waste as urea. This is the first known instance of complete ureotelism in an entirely aquatic teleost fish. Very small 'apparent' ammonia excretion (less than 5% of overall N excretion) was attributable to faecal/bacterial production. Ammonia excretion could not be induced by feeding, reduced temperature, or exposure to pH 7. Exhaustive exercise induced only a small efflux of ammonia. Urea output was inhibited completely by pH 7 water and partly by exhaustive exercise, and greatly stimulated by exposure to 500 mumol/L NH3 (at pH 10). A related species, nominally Oreochromis nilotica, which lives in freshwater at circumneutral pH in the same geographic region, excretes 85% ammonia-N and 15% urea-N at pH 7 in the standard teleost fashion. Urea-N efflux increased to 33% upon transfer of O. nilotica to pH 10 in freshwater. Urea output in this species was only marginally stimulated by exposure to 500 mumol/L NH3 (at pH 7). Plasma and white muscle urea levels were 4- to 5-fold higher in O. a. grahami than in O. nilotica, and plasma levels increased between caudal and cardiac sampling sites, indicating hepatic ureagenesis. Blood pH and PNH3 levels, when corrected for sampling artifact, were unusually high in O. a. grahami. We hypothesize that complete ureotelism in O. a. grahami is an evolutionary response to the problems of excreting ammonia into highly buffered water at pH 10 and/or acid-base balance in this extreme environment.
Wood
C M
CM
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Perry
S F
SF
Wright
P A
PA
Bergman
H L
HL
Randall
D J
DJ
D000136:Acid-Base Equilibrium; D000222:Adaptation, Physiological; D000468:Alkalies / Q000378:metabolism*; D000641:Ammonia / Q000097:blood / Q000378:metabolism*; D000818:Animals; D005243:Feces / Q000032:analysis; D005399:Fishes / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007630:Kenya; D009132:Muscles / Q000032:analysis; D013997:Time Factors; D014508:Urea / Q000097:blood / Q000378:metabolism*; D014867:Water / Q000032:analysis
D000468:Alkalies; D014867:Water; D000641:Ammonia; D014508:Urea