Normalization of protein synthesis in brain cortex of rats after hypoxia by transplantation of embryonic nervous tissue.
It has been shown autoradiographically using 3H-lysine-3H-glycin mixture that acute hypoxic hypoxia leading to mass diffuse dystrophy of brain cortex neurons in rats causes a statistically significant decrease in the level of protein synthesis in cortical neurons. The biochemical study of the same material using 3H-leucine has demonstrated that the overall level of protein synthesis in the total cortical tissue (in nerve and non-nerve cells) is not reduced after hypoxia probably due to a high resistance of glial and other non-nerve cells to oxygen deficiency. Transplantation of embryonic nervous tissue into the brain of rats exposed to hypoxia results not only in normalization of the structure of a part of dystrophic neurons but also in a statistically significant increase in the level of protein synthesis which is retained up to the end of the experiment (i. e. for 120 days following the operation) in the total cortical tissue and reaches the normal value in neurons as established autoradiographically.
Polezhaev
L V
LV
N. I. Vavilov Institute of General Genetics, USSR Academy of Sciences, Moscow.
Alexandrova
M A
MA
Vitvitsky
V N
VN
Cherkasova
L V
LV
Saburina
I N
IN
Girman
S V
SV
D000818:Animals; D001345:Autoradiography; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D009417:Nerve Tissue / Q000196:embryology / Q000637:transplantation*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D009419:Nerve Tissue Proteins