Radioimmunoscintigraphy of human malignant melanoma. I. Pharmacokinetics and localization of 125I-labeled monoclonal antibody RG-12 in thymectomized mice bearing renal melanoma xenotransplants.
The novel RG-12 monoclonal antibody (MoAb) recognizing a high-molecular-weight antigen of human melanoma cells was radioiodinated and its biodistribution and tumor imaging was determined in immunosuppressed mice bearing xenografted human malignant melanoma HMB-2. Control and tumor-bearing mice were injected with 6 micrograms of 125I-labeled RG-12 IgG (8.9 MBq 125I-IgG/animal). Clearance of the MoAb from plasma had a mean half life of 20.6 hours. At day 2 after injection, radiolabeled RG-12 IgG localized in the tumor was 1.43% of the injected dose bound per gram tissue (ID/g), whereas the localization in the healthy kidney was below 0.5%. Tumor to tissue ratio of MoAb accumulation was low for hepatic tissue (1.25) but high for spleen (3.30) and kidney (3.25), respectively. Scanning with a gamma camera localized tumor mass in the right kidney and implanted peritoneal metastases.
Svec
J
J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Veselovská
Z
Z
Keszeghová
V
V
Reinerová
M
M
Makaiová
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D002460:Cell Line; D006207:Half-Life; D006801:Humans; D007074:Immunoglobulin G / Q000493:pharmacokinetics; D007457:Iodine Radioisotopes; D007680:Kidney Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology; D008545:Melanoma / Q000000981:diagnostic imaging* / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010534:Peritoneal Neoplasms / Q000000981:diagnostic imaging; D011877:Radionuclide Imaging; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes