Induction of specific-locus mutations in male mice by ethyl methanesulfonate (EMS).
Using a sequential mating procedure, the induction of specific-locus mutations by ethyl methanesulfonate (EMS) was reinvestigated in male mice. Doses of 175 mg/kg b.w. and 250 mg/kg b.w. of EMS induce gene mutations in the mating intervals 5-8 and 9-12 days post treatment. However, only the frequency of dominant lethal mutations increases with the dose, not the frequency of specific-locus mutations. This observation implies that with a higher dose of EMS a larger fraction of mutagenized spermatozoa and spermatids are selectively eliminated, leading to underestimation of the specific-locus mutation yield at high doses. EMS does not induce specific-locus mutations in spermatogonia.
Ehling
U H
UH
GSF-Institut für Säugetiergenetik, Neuherberg, F.R.G.
Neuhäuser-Klaus
A
A
D000818:Animals; D003433:Crosses, Genetic; D004305:Dose-Response Relationship, Drug; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D005260:Female; D005799:Genes, Dominant; D005804:Genes, Lethal; D008297:Male; D051379:Mice; D009153:Mutagens; D013094:Spermatozoa / Q000187:drug effects
D009153:Mutagens; D005020:Ethyl Methanesulfonate