Effects of interleukin 4 on neonatal B lymphocyte tolerance.
Perturbation of antigen receptors on mouse neonatal B cells by rabbit antimouse IgM antibody was shown to inhibit cell proliferation in response to the B cell mitogen lipopolysaccharide. When these antibody-inactivated cells were challenged with lipopolysaccharide in the presence of the helper T cell product interleukin 4, a strong proliferative response was observed. Interleukin 4 alone did not cause proliferation of the antibody-treated B cells. Pretreatment with interleukin 4 did not prevent neonatal B cell inactivation by the antibody. Our results show that neonatal B cells inactivated directly through their antigen receptors can be reactivated by the combined signals of interleukin 4 and lipopolysaccharide.
Pinto
V B
VB
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33101.
Riley
R L
RL
Peacock
J S
JS
D000818:Animals; D000831:Animals, Newborn; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D015847:Interleukin-4 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000276:immunology
D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D015847:Interleukin-4