Nuclear proteins binding to an enhancer element of the major histocompatibility class I promoter: differences between highly oncogenic and nononcogenic adenovirus-transformed rat cells.
Two major DNA-binding activities specific for the major histocompatibility (MHC) class I regulatory element (CRE) were detected in adenovirus (Ad)-transformed cells. One activity, term CRE1, had similar binding properties to a previously described positive-acting transcription factor specific for MHC class I genes termed H2TF1. The other activity, termed CRE2, bound to a region on the CRE separate from CRE1, and was present in Ad12, but not in Ad5-transformed cells. A CRE2-like activity was also present in non-adenovirus-transformed mouse L929 cells, indicating that CRE2 may be a cellular, rather than a viral, factor. The CRE2 activity did not correspond to any previously described transcription factor with a potential binding site in the CRE.
Ackrill
A M
AM
Department of Biochemistry, University of Leeds, United Kingdom.
Blair
G E
GE
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D004742:Enhancer Elements, Genetic; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D015395:Histocompatibility Antigens Class I; D009687:Nuclear Proteins; D009841:Oligonucleotides