Studies with nonradioisotopic sodium chromate. I. Development of a technique for measuring red cell volume.
A nonradioisotopic method for measuring red cell volume that involves the use of 52Cr-sodium chromate as the red cell label and of graphite furnace atomic absorption analysis of chromium is described. The technique allows the labelling of 20 mL of packed red cells with 40 to 50 micrograms of sodium chromate (Na2CrO4) in 30 minutes at 22 degrees C with 94 +/- 6 percent uptake. Approximately 40 micrograms of Na2CrO4 was injected for in vivo studies. This results in posttransfusion in vivo red cell chromium levels after sample processing in the range of 1 to 7 micrograms per L, which could be quantitated accurately (coefficient of variation = 4.7%) by Zeeman electrothermal atomic absorption spectrophotometry. The labeling concentration of chromium did not cause increased hemolysis, and the labeled cells exhibited an osmotic fragility curve similar to that of unlabeled, fresh ACD red cells. Red cell glutathione peroxidase was unaffected by labeling, although glutathione reductase was reduced by approximately 13 percent (p less than 0.05). The 52Cr red cell volume-measuring method was evaluated by concurrent in vivo studies with the standard 51Cr and 125I-albumin methods for that procedure. Simultaneous measurement of red cell volumes in seven volunteers by the 51Cr, 52Cr, and 125I-albumin techniques correlated highly with each other (r greater than 0.76), with mean values of 2294 +/- 199, 2191 +/- 180, and 2243 +/- 291 mL, respectively. The standard deviations of the differences were small: 134 mL for 52Cr versus 51Cr and 183 mL for 52Cr versus 125I.(ABSTRACT TRUNCATED AT 250 WORDS)
Heaton
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WA
Department of Pathology, Medical College of Hampton Roads, Old Dominion University, Virginia.
Hanbury
C M
CM
Keegan
T E
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Pleban
P
P
Holme
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S
D002840:Chromates; D002857:Chromium / Q000097:blood; D002860:Chromium Radioisotopes; D004911:Erythrocyte Volume; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006801:Humans; D012711:Serum Albumin, Radio-Iodinated; D017670:Sodium Compounds; D013054:Spectrophotometry, Atomic
D002840:Chromates; D002860:Chromium Radioisotopes; D012711:Serum Albumin, Radio-Iodinated; D017670:Sodium Compounds; D002857:Chromium; C028982:sodium chromate(VI); D005979:Glutathione Peroxidase; D005980:Glutathione Reductase