title: Iron-molybdenum cofactor insertion into the Apo-MoFe protein of nitrogenase involves the iron protein-MgATP complex.
abstract: Nitrogenase is composed of two component proteins, the iron protein (Fe protein) and the molybdenum-iron protein (MoFe protein). The Fe protein is a Mr 60,000 dimer of identical subunits with one bridging [4Fe-4S] center. It serves as a one-electron donor to the MoFe protein in a reaction that is coupled to MgATP hydrolysis. The MoFe protein is an alpha 2 beta 2 tetramer of Mr 220,000 which contains four [4Fe-4S] clusters and two iron-molybdenum cofactor (FeMo cofactor) centers. The exact structure of FeMo cofactor is not known, but it is believed to form the active site of the enzyme. Using specifically constructed deletion mutants of Azotobacter vinelandii, we have previously shown that the Fe protein, but not the MoFe protein, is required for FeMo cofactor biosynthesis (Robinson, A. C., Dean, D. R., and Burgess, B. K. (1987) J. Biol. Chem. 262, 14327-14332). During the partial purification of a FeMo cofactor-deficient form of the MoFe protein from one of these mutants (DJ54, delta nifH), we have discovered that, in addition to biosynthesis, the Fe protein-MgATP complex is involved in FeMo cofactor insertion into the MoFe protein. This insertion process is also sensitive to a number of other parameters (e.g. salt, pH, temperature, protein concentration). Based on our experimental data, we present a model for how this insertion reaction might take place, in which the Fe protein-MgATP complex binds the FeMo cofactor-deficient form of the MoFe protein and stabilizes a specific conformation of the MoFe protein that has the FeMo cofactor binding site exposed and available for coordination by preformed FeMo cofactor.
authors: Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
authors: A C
authors: AC
authors: Robinson
authors: T W
authors: TW
authors: Chun
authors: J G
authors: JG
authors: Li
authors: B K
authors: BK
authors: Burgess
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000693:Anaerobiosis; D001059:Apoproteins / Q000378:metabolism; D001395:Azotobacter / Q000378:metabolism*; D002474:Cell-Free System; D005288:Ferredoxins / Q000378:metabolism*; D007700:Kinetics; D046911:Macromolecular Substances; D008983:Molybdoferredoxin / Q000378:metabolism*; D009591:Nitrogenase / Q000378:metabolism*
chemical_list: D001059:Apoproteins; D005288:Ferredoxins; D046911:Macromolecular Substances; D008983:Molybdoferredoxin; D000255:Adenosine Triphosphate; D009591:Nitrogenase