[Induction of cytochrome P-450 in rat liver microsomes by perfluorodecalin].
Perfluorodecalin was incorporated into phospholipid liposomes and injected intraperitoneally in various dozes. The maximal cytochrome P-450 induction is reached 48 hours after perfluorodecalin injection. Cytochrome P-450 content increases 4 times after perfluorodecalin injection in dose of 0.6 ml/kg in homogenate, and 6 times after perfluorodecalin injection in a dose of 0.4 ml/kg in microsomes. Phenobarbital and perfluorodecalin induce several cytochrome P-450 isozymes and cause the appearance of a new isozyme with mass 56 kD absent in microsomes of intact CBA mice. Perfluorodecalin induction strongly increased the rate of NADPH-dependent aminopyrine nN-demethylation (6-7 times per mg of microsomal protein and 1.5 times per nmol cytochrome P-450). The rate of NADPH-dependent hydroxylation of aniline was not affected by perfluorodecalin induction.
Adrianov
N V
NV
Archakov
A I
AI
Tsigler
M
M
D000818:Animals; D001802:Blood Substitutes / Q000008:administration & dosage / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D005466:Fluorocarbons / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008081:Liposomes; D051379:Mice; D008808:Mice, Inbred CBA; D008862:Microsomes, Liver / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology
D001802:Blood Substitutes; D005466:Fluorocarbons; D008081:Liposomes; C008805:perfluorodecalin; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital
Induktsiia perftordekalinom tsitokhroma P-450 v mikrosomakh pecheni mysheĭ.