[Effect of nucleotides on N-methylcytisine and dimethyltubocurarine binding by the nicotinic acetylcholine receptors of the optic ganglia in the squid B. magister].
The effect of nucleosides mono-, di-, and triphosphates on binding of 3H-N-methylcytisine and 14C-tubocurarine to nAChR from squid optical ganglia were investigated. It was found, that ATP and GTP potentiate the specific binding of 3H-N-methylcytisine and inhibit the one of 14C-tubocurarine. While conducting the photoaffinity modification of nACHR by 3H-azidomethylcytisine in the presence of ATP the increase of specific incorporation of label was observed in comparison with control. Molecular weight of labeled receptor complex and subunit, carrying the binding site was the same as the original.
Pliashkevich
Iu G
IuG
Demushkin
V P
VP
D000818:Animals; D002850:Chromatography, Gel; D003596:Cytosine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D049832:Decapodiformes / Q000187:drug effects* / Q000378:metabolism; D005724:Ganglia / Q000187:drug effects* / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D009711:Nucleotides / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D011869:Radioligand Assay; D011950:Receptors, Cholinergic / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D014403:Tubocurarine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
D008565:Membrane Proteins; D009711:Nucleotides; D011950:Receptors, Cholinergic; C031532:1-methylcytosine; D003596:Cytosine; C015836:dimethyltubocurarine; D014403:Tubocurarine
Vliianie nukleotidov na sviazyvanie N-metiltsitizina i dimetiltubokurarina nikotinovymi atsetilkholinovymi retseptorami zritel'nykh gangliev kal'mara B. magister.