In vivo arming of cutaneous mast cell receptors by IgE released from macrophages.
Intracutaneous injection of purified peritoneal macrophages harvested from ovalbumin (OVA)-hypersensitive high-IgE-responder BN rats into naive animals sensitised the injection sites for subsequent OVA-specific passive cutaneous anaphylaxis (PCA) reactions. The underlying mechanism(s) were investigated using a macrophage cell line (WEHI 265.1), which exhibited comparable sensitising activity in rat or mouse skin, after initial pulsing in vitro with antiserum rich in OVA-specific IgE. Transfer of OVA-hypersensitivity by the cell line (1) was IgE-dependent and did not occur when the cells were pre-exposed to antiserum containing OVA-specific IgG alone, (2) was blockable by saturation of cell surface receptors in the recipient with myeloma IgE (but not myeloma IgG), and (3) did not occur in mast cell-deficient mice carrying the W/Wv mutation, in contrast to their normal heterozygous littermates which developed marked OVA-hypersensitivity at the injection site. These results are consistent with arming of IgE-receptors on cutaneous mast cells by IgE antibody released from macrophages, and hint at a possible role for phagocytes in amplifying IgE-mediated reactions in tissues.
Holt
P G
PG
Clinical Immunology Research Unit, Princess Margaret Hospital, Subiaco, Australia.
Bilyk
N
N
Vines
J
J
Turner
K J
KJ
Thomas
W R
WR
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D007114:Immunization; D007073:Immunoglobulin E / Q000502:physiology*; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D009194:Myeloma Proteins / Q000494:pharmacology; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D011914:Rats, Inbred BN; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000276:immunology*
D009194:Myeloma Proteins; D011971:Receptors, Immunologic; D007073:Immunoglobulin E; D010047:Ovalbumin