[Flow cytometric DNA analysis of frozen samples].
Now that flow cytometric DNA analysis is becoming an established procedure, many samples that are being received from various hospitals cannot all be analyzed within a day. Therefore, samples must be stored for subsequent analysis in a flow system. To determine a proper temperature for storage by freezing, samples of the same material were stored for 2 weeks at 5 degrees C, -80 degrees C, and -195 degrees C. Measurement and analysis at the end of this period revealed no major differences among the subsequent histograms of these samples. Thus, it seems that a flow cytometric DNA analysis can be made of frozen samples for diagnostic and prognostic evaluations.
Kawasaki
M
M
Dept. of Pediatric Surgery, Saiseikai Shimonoseki General Hospital.
Sasaki
K
K
Murakami
T
T
Tanaka
K
K
Takahashi
M
M
D015925:Cryopreservation / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D005615:Freezing; D006801:Humans; D014021:Tissue Preservation / Q000379:methods
D004273:DNA, Neoplasm