Chemokinetic effects of exogenous and endogenous tumor necrosis factor alpha on human blood monocytes.
The possible chemokinetic effect of human tumor necrosis factor alpha (TNF-alpha) upon human monocytes was investigated by using a type 1 collagen gel system. Human recombinant TNF-alpha was found to be chemokinetic, and concentrations of that cytokine as low as 0.7 pg/microliters enhanced the movement of those leukocytes. Furthermore, we explored whether endogenous TNF-alpha stimulates monocyte movement by incubating blood monocytes with a TNF-alpha-stimulating agent, human recombinant interleukin 1, with or without antibodies to human TNF-alpha. Human recombinant interleukin 1 enhanced the motility of blood monocytes, and that effect was blocked by rabbit polyclonal antibodies to human TNF-alpha. Furthermore, the movement of blood monocytes no exposed to exogenous cytokines was decreased after incubation with anti-TNF-alpha. Thus, endogenous as well as exogenous TNF-alpha is a chemokinetic agent for human monocytes.
Mushtaha
A A
AA
Department of Pediatrics, University of Texas Medical Branch, Galveston.
Schmalstieg
F C
FC
Hughes
T K
TK
Rudloff
H E
HE
Goldman
A S
AS
D000328:Adult; D000818:Animals; D002630:Chemotactic Factors / Q000494:pharmacology* / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000494:pharmacology* / Q000502:physiology
D002630:Chemotactic Factors; D007375:Interleukin-1; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha