Anti-high endothelial venule monoclonal antibody HECA-452 recognizes plasmacytoid T cells and delineates an "extranodular" compartment in the reactive lymph node.
So-called plasmacytoid T cells represent a subset of monocyte related cells, which share with endothelium the CD36+ CD11b- (OKM5+ OKM1-) phenotype. The reactivity of plasmacytoid T cells with rat monoclonal antibody HECA-452, highly specific for high endothelial venules, was analyzed in reactive lymph nodes. In all cases, HECA-452 not only labelled the endothelium of high endothelial venules, but also strongly reacted with singular and clustered plasmacytoid T cells. The HECA-452 positivity for high endothelial venules and plasmacytoid T cells visualized a lymph node compartment extending from the subcapsular sinus to the corticomedullary junction. This compartment surrounded the composite nodule and was designated the "extranodular" compartment. The co-occurrence of plasmacytoid T cells and high endothelial venules in this extranodular compartment, together with their immunophenotypical similarities, may be indicative of functional co-operations.
Facchetti
F
F
Department of Pathology, Catholic University of Leuven, Belgium.
de Wolf-Peeters
C
C
van den Oord
J J
JJ
Meijer
C J
CJ
Pals
S T
ST
Desmet
V J
VJ
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D004730:Endothelium, Vascular / Q000276:immunology*; D006801:Humans; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D009000:Monocytes / Q000145:classification / Q000276:immunology; D010950:Plasma Cells / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014699:Venules
D000911:Antibodies, Monoclonal