title: Isolation and characterization of an antitumor active agar-type polysaccharide of Gracilaria dominguensis.
abstract: Cold water extraction of the red alga Gracilaria dominguensis, followed by cetyltrimethylammonium bromide fractionation, gave a highly sulfated, agar-type polysaccharide which inhibited the transplantation of Ehrlich ascites carcinoma in mice. The structure of the polysaccharide has been investigated by methylation analysis, and 1H- and 13C-n.m.r. spectroscopy, and was shown to be mainly composed of alternating (1----3)-linked beta-D-galactopyranosyl 6-sulfate and (1----4)-linked 3,6-anhydro-alpha-L-galactopyranosyl residues.
authors: Departamento de Bioquímica, Instituto Nacional de Oncología y Radiobiología, Havana, Cuba.
authors: L E
authors: LE
authors: Fernández
authors: O G
authors: OG
authors: Valiente
authors: V
authors: V
authors: Mainardi
authors: J L
authors: JL
authors: Bello
authors: H
authors: H
authors: Vélez
authors: A
authors: A
authors: Rosado
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000627:therapeutic use; D002236:Carbohydrate Conformation; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy; D002845:Chromatography; D005690:Galactose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D051379:Mice; D015394:Molecular Structure; D011134:Polysaccharides / Q000302:isolation & purification* / Q000627:therapeutic use; D000461:Rhodophyta / Q000032:analysis*; D014461:Ultracentrifugation
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D005690:Galactose