title: Tumour necrosis factor production by IL-2-activated macrophages in vitro and in vivo.
abstract: Purified human and murine monocytes can be activated in vitro by human recombinant IL-2 to secrete tumour necrosis factor (TNF). TNF mRNA is detected within 4 h of addition of IL-2. Signal transduction does not appear to require expression of the Tac peptide of the IL-2 receptor. IL-2 does, however, induce Tac mRNA and Tac peptide expression on peripheral blood monocytes (PBM). In addition to these in vitro studies, PBM retrieved from IL-2-treated cancer patients and mice have been shown to have a greatly increased capacity for TNF production. These studies identify a pathway for macrophage activation by IL-2 both in vitro and in vivo. They also suggest a mechanism for some of the immunoregulatory and toxic effects of IL-2 based immunotherapy regimens.
authors: John Wayne Cancer Clinic, UCLA School of Medicine 90024.
authors: J S
authors: JS
authors: Economou
authors: W H
authors: WH
authors: McBride
authors: R
authors: R
authors: Essner
authors: K
authors: K
authors: Rhoades
authors: S
authors: S
authors: Golub
authors: E C
authors: EC
authors: Holmes
authors: D L
authors: DL
authors: Morton
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D007376:Interleukin-2; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha