title: Determination of overt carnitine palmitoyltransferase by reversed-phase high-performance liquid chromatography.
abstract: A method for the determination of carnitine palmitoyltransferase I (CPT I; EC 2.3.1.19) in isolated rat liver mitochondria by reversed-phase high-performance liquid chromatography is described. Enzyme activity is assayed by direct determination of coenzyme A (CoA) released from palmitoyl-CoA within 60 min by a linear gradient system. CPT 1 in rat liver mitochondria can be assayed from only 30 micrograms of mitochondrial protein per millilitre of assay mixture. The changes in the kinetic parameters of CPT I, including Ki for malonyl-CoA, resulting from the fasting-feeding cycle are also discussed.
authors: Department of Critical Care Medicine, Kansai Medical University, Osaka Japan.
authors: N
authors: N
authors: Takeyama
authors: N
authors: N
authors: Matsuo
authors: D
authors: D
authors: Takagi
authors: T
authors: T
authors: Tanaka
mesh_terms: D000217:Acyltransferases / Q000032:analysis*; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005215:Fasting; D007700:Kinetics; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000217:Acyltransferases; D002334:Carnitine O-Palmitoyltransferase