Reduction of murine interleukin-1 beta: a possible mechanism in the functional maturation of IL-1 beta.
Studies related to the functional activity of human endogenous pyrogen have emphasized its susceptibility to oxidative inactivation. Here we show that the specific activity of murine IL-1 beta polypeptides can be increased by reduction and subsequent alkylation. This treatment induces a change in the relative molecular weight from 20 kDa to 21 kDa of the molecule, suggesting that conformational changes may play a critical role in the functional maturation of IL-1 beta molecules.
Beuscher
H U
HU
Institute of Clinical Microbiology, Erlangen, FRG.
Günther
C
C
Röllinghoff
M
M
D000818:Animals; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008968:Molecular Conformation; D008970:Molecular Weight; D010084:Oxidation-Reduction
D007375:Interleukin-1