Androgen-induced production of colony-stimulating factor (CSF) and colony-inhibitory factor (CIF) in the submandibular gland in female mice.
Both testosterone (T) and 5 alpha-dihydrotestosterone (DHT) significantly increased colony-stimulating factor (CSF) as well as colony-inhibitory factor (CIF) in the submandibular gland (SMG) in female mice. The CIF activity was completely inactivated by heating at 60 degrees C for 15 min, while the CSF activity was stable against the heat treatment. It was found that the CSFs obtained from the androgen-treated female mice and from normal male mice behaved identically in chromatography on hydroxylapatite and Ultro-gel AcA 34 columns. The potency of DHT to increase CSF activity was about 10 times greater than that of T.
Shimizu
M
M
Department of Biochemistry, Hoshi University, Tokyo, Japan.
Sato
J
J
Ishii
T
T
Kanada
T
T
Shinoda
M
M
D000728:Androgens / Q000502:physiology*; D000818:Animals; D002850:Chromatography, Gel; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D013196:Dihydrotestosterone / Q000494:pharmacology; D005260:Female; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D014176:Protein Biosynthesis; D011506:Proteins; D013363:Submandibular Gland / Q000378:metabolism*; D013739:Testosterone / Q000502:physiology
D000728:Androgens; D003115:Colony-Stimulating Factors; D008222:Lymphokines; D011506:Proteins; D013196:Dihydrotestosterone; D013739:Testosterone; C016313:colony inhibiting factor