Effect of glucose on isocitrate lyase in Phycomyces blakesleeanus.
Repression of the synthesis of isocitrate lyase by glucose and/or induction of the synthesis of isocitrate lyase by acetate in Phycomyces blakesleeanus were demonstrated. Both glycerol and ethanol failed to induce isocitrate lyase activity. Furthermore, glucose appeared to cause an in vivo catabolite inactivation of the derepressed enzyme. Isocitrate lyase was inactivated both reversibly and irreversibly by glucose.
Rua
J
J
Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
De Arriaga
D
D
Busto
F
F
Soler
J
J
D000085:Acetates / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction; D004794:Enzyme Repression; D000431:Ethanol / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D007522:Isocitrate Lyase / Q000096:biosynthesis*; D007700:Kinetics; D009090:Mucorales; D007652:Oxo-Acid-Lyases / Q000096:biosynthesis*; D010800:Phycomyces / Q000187:drug effects / Q000201:enzymology*
D000085:Acetates; D000431:Ethanol; D003513:Cycloheximide; D007652:Oxo-Acid-Lyases; D007522:Isocitrate Lyase; D005947:Glucose