The two core sequences of the adenovirus E1A inducible E4 promoter are required for the formation of a specific DNA-protein complex.
We have previously demonstrated that the Adenovirus 2 (Ad2) E4 promoter is activated by an E1A gene product through an inducible enhancer. We now show that several DNA-protein complexes can be identified by gel-shift assay; the formation of one of these complexes involves the two core sequences previously found critical to the promoter activity.
Gilardi
P
P
Institut Gustave Roussy, Villejuif, France.
Stratford
L
L
Egly
J M
JM
Perricaudet
M
M
D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins