Distribution of ricin within the mammalian para-aortic lymph node. II. Comparison of the localization, after intramuscular dosage of colloidal gold-labelled ricin in vivo, with in vitro binding characteristics of the native toxin.
Previous work has shown that, following an intramuscular injection of ricin, the toxin becomes localized within histiocytes in the sinuses of lymph nodes draining the 'wound' site. When ricin labelled with colloidal gold was similarly injected, it was found within the same lymphoid cells as seen with native ricin. Biologically inert Indian ink apparently follows a similar fate, as demonstrated by the appearance of carbon particles within sinus histiocytes, as soon as 1 h after intramuscular injection. When the binding in vitro of Indian ink or ricin toxin to sections of lymph node was examined, ricin was seen to bind to the surfaces of the same sinusoidal cells and also, with a much lower frequency, to follicular lymphocytes, whereas Indian ink failed to bind. This indicated an interaction between ricin and cell membrane components. Moreover, this binding was inhibited markedly by the galactose-containing disaccharide, lactose, a target sugar specified by the lectin binding site of ricin and to a much lesser extent by the monosaccharide mannose.
Griffiths
G D
GD
Department of Forensic Medicine, St James's University Hospital, Leeds, UK.
Leith
A G
AG
Leek
M D
MD
Green
M A
MA
D000818:Animals; D002244:Carbon; D003102:Colloids; D004396:Coloring Agents / Q000378:metabolism; D006046:Gold; D006644:Histiocytes / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007273:Injections, Intramuscular; D007785:Lactose / Q000494:pharmacology; D008198:Lymph Nodes / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008214:Lymphocytes / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008358:Mannose / Q000494:pharmacology; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D012276:Ricin / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism
D003102:Colloids; D004396:Coloring Agents; C028433:chinese ink; D002244:Carbon; D006046:Gold; D012276:Ricin; D007785:Lactose; D008358:Mannose