title: [Methodologic recommendations for the determination of 25-OH vitamin D3 in plasma using HPLC].
abstract: A method for measuring 25-OH D3-vitamin (calcidiol) in plasma samples of cattle is described. The samples were purified using non-polar C 18-cartridges and 25-OH D3 was determined by high-performance liquid chromatography (HPLC) on a silicic acid straight phase liquid chromatography column (retention time 11.5 min). The detection limit for 25-OH D3 is about 0.5 ng injection quantity, the average recovery was 90%, the standard error amounted to 8%.
authors: Sektion Tierproduktion und Veterinärmedizin der Karl-Marx-Universität Leipzig, Wissenschaftsbereich Tierernährungschemie DDR.
authors: M
authors: M
authors: Matthey
authors: H
authors: H
authors: Graf
authors: G H
authors: GH
authors: Richter
authors: G
authors: G
authors: Flachowsky
mesh_terms: D000818:Animals; D002112:Calcifediol / Q000097:blood*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid
chemical_list: D002112:Calcifediol
vernacular_title: Methodische Hinweise zur Bestimmung von 25-OH D3-Vitamin im Plasma mittels HPLC.