Cloning, expression and sequence homologies of cDNA for human gamma enolase.
The nucleotide sequence of the human gamma-enolase mRNA was determined from recombinant cDNA clones. The sequence spans 2273 bp and includes the complete coding region of 1299 bp, a 5'-noncoding region of 74 bp and a 897-bp-long 3'-noncoding region containing a variant polyadenylation signal (ATTAAA). The deduced amino acid (aa) sequence is 433 aa long and shows a 97% similarity with rat gamma-enolase. Both the 5'- and 3'-untranslated regions are similar (82% and 68%, respectively) to the analogous regions of the rat gamma-enolase gene, suggesting that a strong selective pressure operates on noncoding segments of gamma-enolase mRNAs. The size of the gamma-enolase mRNA expressed in human brain is 2.4 kb. A crosshybridizing 1.5-kb message is detected in human skeletal muscle which may be derived from the beta-enolase-coding gene.
Oliva
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D
Istituto di Biologia dello Sviluppo (Consiglio Nazionale delle Ricerche), Palermo, Italy.
Barba
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G
Barbieri
G
G
Giallongo
A
A
Feo
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010751:Phosphopyruvate Hydratase / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D010751:Phosphopyruvate Hydratase