Inhibitory effects of alpha-carotene on proliferation of the human neuroblastoma cell line GOTO.
alpha-Carotene inhibited the proliferation of the human neuroblastoma cell line GOTO in a dose- and time-dependent manner. In addition, it was about 10 times more inhibitory than beta-carotene. Northern blot analysis indicated that alpha-carotene caused maximum suppression of the level of the N-myc messenger RNA of GOTO cells. This suppression occurred within 18 hours of alpha-carotene treatment, after which the level of the N-myc messenger RNA gradually recovered to the basal level. Analysis by flow cytometry indicated that when GOTO cells were exposed to alpha-carotene, they were arrested in the G0-G1 phase of their cell cycle. However, as the level of the N-myc messenger RNA was recovering, these cells resumed normal cycling. These results indicate that the reduction in the level of the N-myc messenger RNA caused by alpha-carotene is closely linked with G0-G1 arrest.
Murakoshi
M
M
Department of Biochemistry, Kyoto Prefectural University of Medicine, Japan.
Takayasu
J
J
Kimura
O
O
Kohmura
E
E
Nishino
H
H
Iwashima
A
A
Okuzumi
J
J
Sakai
T
T
Sugimoto
T
T
Imanishi
J
J
D002338:Carotenoids / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007399:Interphase / Q000187:drug effects; D009447:Neuroblastoma / Q000473:pathology*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured; D019207:beta Carotene
D012333:RNA, Messenger; D019207:beta Carotene; D002338:Carotenoids; C041635:alpha-carotene