Development of a delayed-type hypersensitivity granuloma model in the mouse for the study of chronic immune-mediated inflammatory disease.
A cutaneous model of delayed-type hypersensitivity granuloma (DTH GRA) was developed, using methylated bovine serum albumin (mBSA) which could be easily quantified and represented chronic cellular immune-mediated inflammation characteristic of rheumatoid arthritis (RA). Quantitative kinetics showed maximal DTH GRA "wet" tissue weights at d.5 post-induction, diminishing between d.15-22; DTH GRA "dry" tissue weights peaked between d.8-22. Histological evaluation revealed early extensive fibrin deposition, edema, mixed PMN/mononuclear leucocyte infiltration and angiogenesis (d.2-5), followed by increased macrophage recruitment, lymphocyte infiltration, angiogenesis and vasculitis (d.10-15). Fibrin resorption and replacement by fibrous tissue resulted in resolution by d.35. It is suggested that the DTH GRA reaction represents a relevant model for probing pathogenic mechanisms and potential therapeutics for RA.
Dunn
C J
CJ
Department of Hypersensitivity Diseases Research, Upjohn Company, Kalamazoo, MI 49001.
Gibbons
A J
AJ
Miller
S K
SK
D000818:Animals; D002908:Chronic Disease; D003872:Dermatitis / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D006099:Granuloma / Q000276:immunology* / Q000473:pathology; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000473:pathology; D007249:Inflammation / Q000276:immunology* / Q000473:pathology; D051379:Mice; D012710:Serum Albumin, Bovine / Q000276:immunology; D012867:Skin / Q000473:pathology; D013997:Time Factors
D012710:Serum Albumin, Bovine