EGF-induced PGE2 release is synergistically enhanced in retinoic acid treated fetal rat lung cells.
Retinoic acid has been shown to induce a 2.5-fold increase in 125I-EGF binding capacity through increased EGF receptor synthesis in a fetal rat lung (FRL) cell line (1). In FRL cells, incubation with either EGF or retinoic acid induces a modest increase in PGE2 secretion (80% or 40%, respectively). However, in the presence of both EGF and retinoic acid, FRL cells exhibit a 6.4-fold increase in PGE2 secretion. Retinoic acid and EGF dose-response curves demonstrate that the effect on PGE2 secretion correlates with the retinoic acid induced increase in EGF receptors. These data suggest a relationship between increased EGF receptor expression and increased EGF responsiveness. Furthermore, these data indicate a potential mechanism by which EGF and retinoic acid may interact in lung physiology.
Oberg
K C
KC
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146.
Carpenter
G
G
D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D015232:Dinoprostone