title: Method of measuring liquid junction potentials in small (nanolitre) droplets of biological fluids.
abstract: 1. A method is described which allows the determination of liquid junction potentials between biological fluid samples of less than 1 nL with a reproducibility of 10%. This was achieved by use of a micro-method and the use of 3% agarose added to the electrolyte in the measuring electrodes. 2. Validation was performed with a modified flowing boundary method and the system was also tested using a solution containing divalent ions. Liquid junction potential between surface fluid from rat kidneys and harvested renal proximal tubular fluids was -0.62 +/- 0.11 mV (mean +/- s.d., n = 66).
authors: Department of Medicine, Sydney University, New South Wales, Australia.
authors: E
authors: E
authors: Sugo
authors: A Z
authors: AZ
authors: Györy
mesh_terms: D001826:Body Fluids / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004566:Electrodes; D004573:Electrolytes / Q000032:analysis; D012834:Silver / Q000032:analysis; D018030:Silver Compounds
chemical_list: D004573:Electrolytes; D018030:Silver Compounds; D012834:Silver; C037548:silver chloride