Dot-enzyme-linked immunosorbent assay (dot-ELISA) for the diagnosis of schistosomiasis.
We describe a dot enzyme-linked immunosorbent assay (dot-ELISA) as a field applicable tool for the rapid diagnosis of schistosomiasis. This antibody capture assay is performed using 50 ng protein of crude Schistosoma mansoni egg antigen (SEA) in 1 microliter volumes per dot. Sera (1 microliter/dot) at 1:40 dilution were optimal. Dot-ELISA results were completely comparable to micro-ELISA.
Hamadto
H A
HA
Kamal
K A
KA
D000293:Adolescent; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008297:Male; D012547:Schistosoma / Q000276:immunology; D012552:Schistosomiasis / Q000175:diagnosis*
D000909:Antibodies, Helminth