Human seminal ribonuclease. Immunological quantitation of cross-reactive enzymes in serum, urine and seminal plasma.
The distribution of secretory-type ribonuclease in human serum, urine and seminal plasma has been studied by immunological measurements. Inhibition of enzyme activity by antibodies against pure human seminal RNAase shows that a cross-reactive enzyme is predominant (90%) in seminal plasma and is a significant component (70-80%) in urine and serum. A competitive binding radioimmunoassay has been developed by using specific antibodies and 125I-labelled RNAase as radioligand. The procedure, very sensitive, reproducible and specific, has been used to determine seminal RNAase levels in seminal plasma samples from 48 healthy individuals (age range, 20-58 years). The mean concentration of the enzyme was found to be 6.6 micrograms/ml (S.D. +/- 1.9).
Sorrentino
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Dipartimento di Chimica Organica e Biologica, Facoltà di Scienze, Università di Napoli, Italy.
De Prisco
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Libonati
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D000328:Adult; D000906:Antibodies / Q000032:analysis* / Q000494:pharmacology; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003429:Cross Reactions; D006801:Humans; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D012260:Ribonucleases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000097:blood / Q000652:urine; D012661:Semen / Q000032:analysis / Q000201:enzymology*
D000906:Antibodies; D012260:Ribonucleases