[Isolation of monoclonal antibodies from mouse ascitic fluid using chromatography on "blue" DEAE cellulose].
A method is described of the isolation of monoclonal antibodies form mouse ascites fluids by chromatography on beaded DEAE cellulose with covalently bound Reactive Blue-2 ("Blue" DEAE cellulose). The method yields antibodies of 80-90% purity, free from protease activity.
Hrkal
Z
Z
Rejnková
J
J
Stöckbauer
P
P
Rybák
M
M
Benes
M J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001202:Ascitic Fluid / Q000276:immunology*; D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D051379:Mice
D000911:Antibodies, Monoclonal
Izolace monoklonálních protilátek z mysích ascitických tekutin chromatografií na "modré" DEAE celulóze.