T cells sensitized to synthetic HLA-DR3 peptide give evidence of continuous presentation of denatured HLA-DR3 molecules by HLA-DP.
T cell clones raised against a synthetic peptide, identical to the third hypervariable region of the DR3 BI chain, were tested for secondary proliferative responses against a panel of PBLs. All seven DR3 DPw3+ stimulators could induce proliferation. DR3- DPw3+ PBLs were recognized when the synthetic peptide was added to the cultures. Inhibition studies with mAbs showed that in both cases the HLA-DP molecule is involved in the recognition of both types of stimulators. We conclude that the clones recognize the DR3 peptide presented by HLA-DPw3. This stimulus can be obtained in two different ways: (a) by addition of synthetic peptide to DPw3+ PBLs or (b) by using DR3 DPw3+ stimulator cells where DR3 peptides are present in the culture as a product of denaturation of the DR3 molecule. Because all DR3 DPw3+ PBLs tested could stimulate the line and clones, we assume that the presentation of the DR3 peptide by DP is a naturally and continuously occurring phenomenon.
de Koster
H S
HS
Department of Immunohematology and Blood Bank, University Hospital, Leiden, The Netherlands.
Anderson
D C
DC
Termijtelen
A
A
D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D006682:HLA-DP Antigens / Q000276:immunology*; D059868:HLA-DP beta-Chains; D006684:HLA-DR Antigens / Q000276:immunology*; D015803:HLA-DR3 Antigen; D006801:Humans; D011489:Protein Denaturation; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C055961:HLA-DPw3 antigen; D006684:HLA-DR Antigens; D015803:HLA-DR3 Antigen