Determination of baclofen and alpha-baclofen in rat liver homogenate and human urine using solid-phase extraction, o-phthalaldehyde-tert.-butyl thiol derivatization and high-performance liquid chromatography with amperometric detection.
Methods were developed for the determination of the zwitterionic compounds baclofen and alpha-baclofen in complex biological samples (rat liver homogenates and human urine) in concentration ranges that would be suitable for pharmacokinetic studies of these compounds. In the procedure, the biological samples along with an internal standard were selectively concentrated using C18 solid-phase extraction cartridges, evaporated, derivatized with o-phthalaldehyde and tert.-butyl thiol at room temperature for 2 min, then subjected to high-performance liquid chromatographic analysis. The reversed-phase (C18) chromatographic analysis with amperometric detection (glassy carbon electrode in oxidative mode, +0.6 V vs. Ag/AgCl) was found to be useful for the measurement of both baclofen and alpha-baclofen from 10 ng/ml to 10 micrograms/ml in these complex biological samples. The primary advantage of the method was that the derivatives formed using tert.-butyl thiol were markedly more stable than the previously reported derivatives prepared using mercaptoethanol.
Wall
G M
GM
Department of Medicinal Chemistry, School of Pharmacy, University of Mississippi, University 38677.
Baker
J K
JK
D000818:Animals; D001418:Baclofen / Q000032:analysis* / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006207:Half-Life; D006801:Humans; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis*; D051381:Rats; D013438:Sulfhydryl Compounds; D009764:o-Phthalaldehyde
D007202:Indicators and Reagents; D013438:Sulfhydryl Compounds; C061330:tert-butylthiol; D009764:o-Phthalaldehyde; D001418:Baclofen