Anti-idiotypic antibodies against a monoclonal antibody specific for the trichothecene mycotoxin T-2.
A BALB/c murine monoclonal antibody against the trichothecene mycotoxin T-2 was generated. The antibody, designated HD11, specifically bound T-2 mycotoxin. The binding of HD11 to T-2 conjugated to bovine serum albumin was inhibited by free T-2 toxin but not by the water-soluble heterocyclic guanidines saxitoxin and tetrodotoxin. The T-2 detection limit in an enzyme-linked immunosorbent assay with HD11 was in the nanogram range. The in vitro cytotoxicity of T-2, as measured by the inhibition of radiolabeled leucine uptake of the human epidermoid carcinoma Hep-2 and KB cell lines, was completely reversed by the addition of HD11. Rabbit anti-idiotypic antibodies specific for HD11 were generated and characterized.
Chanh
T C
TC
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78284.
Huot
R I
RI
Schick
M R
MR
Hewetson
J F
JF
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D012530:Saxitoxin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D012717:Sesquiterpenes / Q000276:immunology*; D013605:T-2 Toxin / Q000032:analysis / Q000276:immunology* / Q000494:pharmacology; D013779:Tetrodotoxin / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D012717:Sesquiterpenes; D012710:Serum Albumin, Bovine; D012530:Saxitoxin; D013779:Tetrodotoxin; D013605:T-2 Toxin