[Hybridomas producing monoclonal antibodies to Crimean hemorrhagic fever virus].
A series of hybridomas producing monoclonal antibodies to Crimean hemorrhagic fever virus was obtained and their cultural properties were characterized. HEMA-12 and HEMA-24 secreted monoclonal IgG2b antibodies, HEMA-101 secreted monoclonal IgG1 antibodies, HEMA-31, HEMA-9 and HEMA-11 secreted monoclonal IgG2 antibodies. According to the results of the indirect immunofluorescence test, the titer of specific immunoglobulins in the culture fluid was 1:16-1:32, but sometimes reached 1:64-1:128. The titer of antibodies in ascitic fluid amounted to dilutions of 10(4)-10(5). Hybridomas were cloned by the method of ultimate dilutions. The injection of 5-15 million HEMA cells into the abdominal cavity of BALB/c mice induced the formation of ascitic tumors in the animals within 7-11 days and the accumulation of ascitic fluid in a volume of 1-4 ml. Hybridomas, found to be capable of passage from mouse to mouse, underwent 5-16 passages during the term of observation.
Shutkova
T M
TM
Mel'nikova
E E
EE
Gaĭdamovich
S Ia
SIa
Novokharskiĭ
A S
AS
Turchinskaia
A L
AL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D006479:Hemorrhagic Fever, Crimean / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D007136:Immunoglobulins / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins
Gibridomy, produtsiruiushchie monoklonal'nye antitela k virusu Krymskoĭ gemorrhagicheskoĭ likhoradki.