Interleukin 1 production by alveolar macrophages is decreased in smokers.
To evaluate the mechanism by which cigarette smoking suppresses pulmonary immune responses, we determined the capacity of alveolar macrophages (AM) to produce interleukin 1 (IL-1 in 32 normal subjects and in 40 patients with pulmonary sarcoidosis. The amount of IL-1 released from LPS-stimulated AM from smokers was significantly decreased compared with that in nonsmokers in both normal and sarcoid groups. The addition of indomethacin to the cultures in 18 normal subjects and in 22 patients with pulmonary sarcoidosis yielded similar results, thus excluding the possibility that this difference resulted from a difference in the amount of cyclooxygenase metabolites released in the culture supernatants. Similar results were obtained by enzyme-linked immunosolvent assay. Because IL-1 is thought to induce the accumulation of T cells at the site of disease and contribute to local cellular and humoral immunity of the lung, our data suggest that the reduced capacity of AM to release IL-1 in smokers affords partial protection against the initiation of immune responses in the lung and the development of granulomatous lung diseases.
Yamaguchi
E
E
First Department of Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
Okazaki
N
N
Itoh
A
A
Abe
S
S
Kawakami
Y
Y
Okuyama
H
H
D000328:Adult; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008171:Lung Diseases / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011650:Pulmonary Alveoli / Q000166:cytology*; D012507:Sarcoidosis / Q000378:metabolism; D012907:Smoking / Q000378:metabolism*
D007375:Interleukin-1