[Protection by 16,16-dimethyl PGE2 of surface epithelial, parietal, and chief cells isolated from rat stomach].
Damage caused by ethanol and its prevention by 16,16-dimethyl PGE2 were tested in fractions rich in surface epithelial cells (F1), rich in chief cells (F2), and rich in parietal cells (F3) isolated from rat stomach in vitro. Ethanol caused cell damage in F1 in a dose-related way and the concentration of ethanol for induction of 50% cell damage was 14.93%. There was no difference in cell damage caused by 15% ethanol between the three different cell fractions. 16,16-Dimethyl PGE2 (10(-6)M) significantly inhibited the damage caused by 15% ethanol in all the three fractions. These results suggest that 16,16-dimethyl PGE2 has direct protective effect on gastric cells and this action is not limited to the specific cell type.
Arakawa
T
T
Fukuda
T
T
Nakamura
A
A
Satoh
H
H
Nakamura
H
H
Kobayashi
K
K
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D000431:Ethanol / Q000009:adverse effects; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D008297:Male; D010295:Parietal Cells, Gastric / Q000187:drug effects* / Q000473:pathology; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D011459:Prostaglandins E, Synthetic; D000431:Ethanol; D015064:16,16-Dimethylprostaglandin E2