[Methodologic recommendations for the determination of 25-OH vitamin D3 in plasma using HPLC].
A method for measuring 25-OH D3-vitamin (calcidiol) in plasma samples of cattle is described. The samples were purified using non-polar C 18-cartridges and 25-OH D3 was determined by high-performance liquid chromatography (HPLC) on a silicic acid straight phase liquid chromatography column (retention time 11.5 min). The detection limit for 25-OH D3 is about 0.5 ng injection quantity, the average recovery was 90%, the standard error amounted to 8%.
Matthey
M
M
Sektion Tierproduktion und Veterinärmedizin der Karl-Marx-Universität Leipzig, Wissenschaftsbereich Tierernährungschemie DDR.
Graf
H
H
Richter
G H
GH
Flachowsky
G
G
D000818:Animals; D002112:Calcifediol / Q000097:blood*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid
D002112:Calcifediol
Methodische Hinweise zur Bestimmung von 25-OH D3-Vitamin im Plasma mittels HPLC.