A sensitive IL-1 thymocyte co-stimulator assay using a novel beta-D-galactoside specific lectin from the beetle, Allomyrina dichotoma.
A sensitive thymocyte co-stimulator assay of IL-1 using a beta-D-galactoside specific lectin (allo A) obtained from the beetle (Allomyrina dichotoma) is reported here. Allo A stimulated [3H]thymidine uptake of mouse thymocytes in the presence of IL-1. The allo A assay was more sensitive than the PHA or PNA- thymocyte assay, especially at low doses of IL-1. Optimal conditions for the allo A assay were as follows: allo A, 2.5-5.0 micrograms/ml; whole thymocytes, 0.5-1.0 x 10(6) cells/well; incubation time, 72-96 hr. The assay is sensitive and convenient and can easily be performed in any laboratory.
Sakai
Y
Y
Department of Parasitology, Yamagata University School of Medicine.
Kimura
S
S
Yamashita
T
T
Umetsu
K
K
Suzuki
T
T
Sendo
F
F
D000818:Animals; D001681:Biological Assay / Q000379:methods; D001517:Coleoptera / Q000032:analysis; D007375:Interleukin-1 / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D037102:Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D009000:Monocytes / Q000032:analysis; D010529:Peritoneal Cavity / Q000166:cytology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000502:physiology*; D013936:Thymidine
C045794:Allomyrina lectin; D007375:Interleukin-1; D037102:Lectins; D010835:Phytohemagglutinins; D011994:Recombinant Proteins; C028784:phytohemagglutinin-P; D013936:Thymidine