Simultaneous determination of lactate, pyruvate, and ascorbate in microdialysis samples from rat brain, blood, fat, and muscle using high-performance liquid chromatography.
An isocratic high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of lactate, pyruvate, and ascorbate in tissue and blood, sampled in vivo using the microdialysis technique. The compounds were separated on a silica-based, reversed-phase column or on a resin-based, cation exchange column. The acids were monitored by ultraviolet detection.
Hallström
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Department of Pharmacology, Karolinska Institutet, Carnegie Medicin AB, Stockholm, Sweden.
Carlsson
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A
Hillered
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L
Ungerstedt
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U
D000273:Adipose Tissue / Q000032:analysis*; D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis* / Q000097:blood; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D007773:Lactates / Q000032:analysis* / Q000097:blood; D009132:Muscles / Q000032:analysis*; D011773:Pyruvates / Q000032:analysis* / Q000097:blood; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D007773:Lactates; D011773:Pyruvates; D001205:Ascorbic Acid