title: Histamine synthesis by mouse T lymphocytes through induced histidine decarboxylase.
abstract: When spleen cells of C57BL/6 mice or mast cell-deficient W/Wv mice were cultured, their histidine decarboxylase (HDC) activity increased with increases in the histamine concentration in the cells and the medium. Addition of concanavalin A (Con A) or Escherichia coli lipopolysaccharide (LPS) enhanced the increase. The removal of adherent cells reduced both the control HDC activity and the response to the mitogens. Purified T lymphocytes responded to Con A but not to LPS. Neither Con A nor LPS had any effect on B lymphocytes. Treatment of T cells with anti-Thy-1.2 and complement completely abrogated the induction of HDC. Histamine synthesis dependent on Con A by T cells was stimulated by the addition of conditioned medium from peritoneal adherent cells activated with LPS. The addition of recombinant interleukin-1 (rIL-1) or peritoneal adherent cells fixed with paraformaldehyde significantly enhanced HDC induction dependent on Con A in T cells. These results suggest that histamine is synthesized by T lymphocytes through HDC and that the reaction was enhanced by a soluble factor(s) released from macrophages.
authors: Department of Nutritional Regulation, Research Institute for Biochemical Regulation, Nagoya University, Chikusa, Japan.
authors: R
authors: R
authors: Aoi
authors: I
authors: I
authors: Nakashima
authors: Y
authors: Y
authors: Kitamura
authors: H
authors: H
authors: Asai
authors: K
authors: K
authors: Nakano
mesh_terms: D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002478:Cells, Cultured; D003208:Concanavalin A; D006632:Histamine / Q000096:biosynthesis*; D006640:Histidine Decarboxylase / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D003208:Concanavalin A; D006632:Histamine; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase