[Toxicity of cyclosporin A for corneal endothelium in tissue culture].
The immunosuppressive agent cyclosporin A (Cy-A) was applied topically for corneal diseases and corneal grafting. In human and animal studies, concentrations of 2%-4% have been used. As the endothelial concentration of the substance is not known, the toxic concentration, especially for the corneal endothelium, has not yet been determined. Using monolayer cultures of porcine corneal endothelium, we studied the effects of dilutions of Cy-A powder and analogous dilutions of Cy-A injection solutions in culture medium ranging from 1:10(2) to 1:10(9). Preconfluent cultures of porcine corneal endothelium were exposed to the test and control media. The cell densities, cell morphology and cell viability were monitored at predetermined intervals. In the control cultures, an increase in cell density was recorded from 250 mm2 to more than 1000/mm2 within 4 days. In the test cultures, a dose-dependent toxic effect was observed which ranged from vacuolic degenerative changes to complete necrosis of the cell monolayer. Based on our results, we conclude that, in topical application, a toxic effect of Cy-A can only be expected at local endothelial concentrations above 1:10(7).
Böhnke
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Heyne-Aufmkolk
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D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D046508:Culture Techniques; D003524:Cyclosporins / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004728:Endothelium, Corneal / Q000187:drug effects*; D013552:Swine
D003524:Cyclosporins
Untersuchungen zur Toxizität von Cyclosporin-A für Hornhautendothel in der Gewebekultur.