[Photolysis of total histone and pBr-322 plasmid DNA in the ultraviolet vacuum region of the spectrum].
The action of VUV and far UV (lambda greater than 240 nm) radiation of aqueous solutions of total histone by means of electrophoresis method is compared. By VUV photolysis the more effective destruction of HI and HIA histone fractions is determined. It may be explained by interaction of total histone with water radicals (H, OH) formed by VUV photodissociation (indirect mechanism). By VUV irradiation of plasmid pBR-322 DNA in aqueous solutions the indirect action mechanism of single strand breaks in DNA is established.
Kiseleva
M N
MN
Tsyganenko
N M
NM
Smirnova
T M
TM
Dodonova
N Ia
NIa
D004247:DNA / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006657:Histones / Q000378:metabolism*; D010782:Photolysis; D010957:Plasmids; D013056:Spectrophotometry, Ultraviolet
D006657:Histones; D004247:DNA
Fotolysis total'nogo gistonea i DNK plazmidy pBR-322 v vakuumnoĭ ul'trafioletovoĭ oblasti spectra.