[Effect of lymphokines produced by human T-T hybridomas on the proliferation of normal and neoplastic B lymphocytes].
Human T-T hybridomas were obtained by fusing T lymphoblasts of the azaguanine-resistant CEM-6 cell line and PHA-activated normal T lymphocytes. After 4 weeks of culture with selective media, proliferating hybrid cells were cloned by limiting dilution. Several clones were obtained and one of them (F2) was shown to secrete a lymphokine with inhibitory activity on the proliferation of an EBV-transformed B cell line, of normal B lymphocytes, as well as of mitogen activated normal T lymphocytes. The proliferation of three other cell lines (MLA-144, CEM-6 and K-562) was not affected. The inhibition of cell proliferation was not due to a cytotoxic effect. The soluble factor from F2 hybridoma was very sensitive to heat-treatment, was not inactivated by target cell adsorption and, finally, it was effective at very low concentrations (70% inhibition at 1:1,000 dilution, v/v). The suppressor activity in the supernatant from F2 hybridoma did not appear to be related to other known lymphokines.
Gambatesa
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D001402:B-Lymphocytes / Q000166:cytology* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis / Q000502:physiology*
D006131:Growth Inhibitors; D008222:Lymphokines
Influenza di linfochine prodotte da ibridomi umani T-T sulla proliferazione di linfociti B normali e neoplastici.