title: Stimulation of mouse lymphocytes by a mitogen derived from Mycoplasma arthritidis. VII. Responsiveness is associated with expression of a product(s) of the V beta 8 gene family present on the T cell receptor alpha/beta for antigen.
abstract: Mycoplasma arthritidis produces a potent soluble T lymphocyte mitogen (MAM) which is dependent upon accessory cells bearing the alpha-chain of the I-E molecule (E alpha). Lymphocytes from the RIIIS mouse strain which possess E alpha yet whose T cells fail to recognize the MAM-E alpha complex were shown not to express V beta 8.1, 8.2 or 8.3 gene products present on the TCR-alpha/beta by virtue of their lack of reactivity with the F23.1 mAb. Because lymphocytes from the congenic B10.RIII mouse strain reacted positively with F23.1, we examined the progeny from (RIIIS x B10.RIII)F1 x RIIIS backcross mice for cosegregation of lymphocytes expressing F23.1-reactive sites and ability to proliferate in response to MAM. Whereas lymphocytes of all mice responded to Con A, only lymphocytes from progeny expressing F23.1-reactive cells responded to MAM. Similar studies were conducted on progeny from the F23.1- SWR mouse which was backcrossed to (SWR x B10.RIII)F1 mice. Of the E alpha-bearing progeny, there was a direct correlation between lymphocyte expression of F23.1 determinants and ability to respond to MAM. These results established that MAM reactivity was dependent upon a product(s) of the V beta locus of the TCR-alpha/beta. Nodal and thymic lymphocytes cultured for 3 days in vitro with MAM exhibited clonal expansion of F23.1 and F23.2-reactive cells as compared with cultures treated with Con A. We also demonstrated that F23.1 and F23.2 mAb inhibited the ability of lymphocytes to proliferate in response to MAM but had little effect on responses to Con A. The combined data suggest that the MAM-E alpha complex can utilize a V beta 8 gene product(s) on the TCR-alpha/beta.
authors: Department of Medicine, University of Utah School of Medicine, Salt Lake City 84132.
authors: B C
authors: BC
authors: Cole
authors: D R
authors: DR
authors: Kartchner
authors: D J
authors: DJ
authors: Wells
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000941:Antigens; D000942:Antigens, Bacterial; D005260:Female; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology; D007166:Immunosuppressive Agents / Q000502:physiology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008934:Mitogens / Q000494:pharmacology*; D005810:Multigene Family; D009174:Mycoplasma / Q000276:immunology*; D011506:Proteins; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D018089:Superantigens; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000941:Antigens; D000942:Antigens, Bacterial; D007135:Immunoglobulin Variable Region; D007166:Immunosuppressive Agents; D008934:Mitogens; C052996:Mycoplasma arthritidis mitogen; D011506:Proteins; D011948:Receptors, Antigen, T-Cell; D018089:Superantigens