IgG anti-IgE from atopic dermatitis induces mediator release from basophils and mast cells.
IgG antibodies containing anti-IgE activity isolated from a patient with atopic dermatitis (H-aIgE) induced mediator release from human basophils and mast cells isolated from skin and lung tissues. The release of histamine was calcium- and temperature-dependent and did not involve cytotoxicity. There was an excellent correlation (r = 0.88; p less than 0.001) between the maximum percent histamine release from human basophils induced by rabbit anti-IgE (R-aIgE) and H-aIgE. H-aIgE was approximately 30 times more potent than R-aIgE in inducing mediator release from human basophils, skin, and lung mast cells. H-aIgE specifically desensitized human basophils to a subsequent challenge with both H-aIgE and R-aIgE. Lactic acid removal of IgE from human basophils blocked the releasing activity of both R-aIgE and H-aIgE. Passive sensitization with hyperimmune sera or purified IgE myeloma restored the response of basophils to both R-aIgE and H-aIgE. IgE purified from three different myeloma patients concentration-dependently blocked the histamine releasing activity of both R-aIgE and H-aIgE.
Marone
G
G
Department of Medicine, University of Naples II School of Medicine, Italy.
Casolaro
V
V
Paganelli
R
R
Quinti
I
I
D000293:Adolescent; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001491:Basophils / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000097:blood / Q000276:immunology*; D003888:Desensitization, Immunologic; D006632:Histamine / Q000378:metabolism; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008407:Mast Cells / Q000378:metabolism*; D013189:SRS-A / Q000096:biosynthesis; D012867:Skin / Q000166:cytology / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D013189:SRS-A; D007073:Immunoglobulin E; D006632:Histamine