Placental protein 14 (PP14) inhibits the synthesis of interleukin-2 and the release of soluble interleukin-2 receptors from phytohaemagglutinin-stimulated lymphocytes.
Crude human decidual extracts containing up to 15.0 mg/l PP14 were investigated for their effects on the release of interleukin-2 (IL-2) and of IL-2 receptor (IL-2R) from phytohaemagglutinin (PHA) stimulated lymphocytes. The crude decidual extract suppressed IL-2 production over the culture period investigated (0-90 h) with maximal suppression observed at around 66 h of culture. The suppression was dose dependent over the range of PP14 concentrations used (0-7.0 mg/l). Decidual extracts also inhibited the release of soluble IL-2R into the culture supernatants over the same period. In both cases, the specific reduction of the PP14 content of the extracts by a monoclonal anti-PP14 immunoadsorbent reduced the observed suppression. These results suggest that PP14 inhibits the production of IL-2 from mitogenically stimulated lymphocytes, and leads to a reduced IL-2R release. The mechanism for such an effect is as yet unknown; however, it may explain the previously reported immunosuppressive activity of PP14.
Pockley
A G
AG
Department of Biomedical Sciences, Sheffield City Polytechnic, England.
Bolton
A E
AE
D002478:Cells, Cultured; D003864:Depression, Chemical; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D011257:Pregnancy Proteins / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000378:metabolism*
D000071816:Glycodelin; D006023:Glycoproteins; D007376:Interleukin-2; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D015375:Receptors, Interleukin-2