Identification of HIV-1 infected seropositive subjects by a direct diagnostic test involving hybridization of amplified viral DNA.
We describe a simple slot-blot hybridization procedure with an oligo-probe for identification of amplified HIV-1 DNA in seropositive subjects. Comparison are realized, on the same DNA samples, with other methods DNA detection including Southern blot tests after hybridization with a labelled probe, and autoradiographic patterns after digestion with a restriction enzyme of the amplified product hybridized. This new direct approach towards diagnosis of HIV-1 infection easily be carried out on a large scale.
Lucotte
G
G
Laboratoire d'Anthropologie Physique, Collège de France, Paris.
Reveilleau
J
J
D015139:Blotting, Southern / Q000379:methods; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005784:Gene Amplification; D005814:Genes, Viral; D015483:HIV Antibodies / Q000032:analysis; D006679:HIV Seropositivity / Q000175:diagnosis*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012016:Reference Values
D004279:DNA, Viral; D015483:HIV Antibodies; D015345:Oligonucleotide Probes