Inhibition of the replication of hepatitis B virus by the carbocyclic analogue of 2'-deoxyguanosine.
We report that treatment of 2.2.15, a human hepatoblastoma-derived cell line in which hepatitis B virus is actively replicating, with the carbocyclic analogue of 2'-deoxyguanosine [Shealy, Y. F., O'Dell, C. A., Shannon, W. M. & Arnett, G. (1984) J. Med. Chem. 27, 1416-1421] resulted in the nearly complete cessation of viral replication, as monitored by the absence of both intracellular episomal and secreted viral DNAs and by the absence of viral DNA polymerase activity. The drug was nontoxic in concentrations up to 200 times the minimum effective inhibitory concentration.
Price
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PM
Department of Biochemistry, Mount Sinai School of Medicine, New York, NY 10029.
Banerjee
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Acs
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D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000187:drug effects* / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification; D003849:Deoxyguanosine / Q000494:pharmacology / Q000031:analogs & derivatives; D006515:Hepatitis B virus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D006801:Humans; D008970:Molecular Weight; D012367:RNA, Viral / Q000187:drug effects / Q000302:isolation & purification; D014779:Virus Replication / Q000187:drug effects*; D015215:Zidovudine / Q000494:pharmacology
D000998:Antiviral Agents; D004279:DNA, Viral; D012367:RNA, Viral; D015215:Zidovudine; C061610:2-amino-1,9-dihydro-9-(3-hydroxy-4-(hydroxymethyl)cyclopentyl)-6H-purine-6-one; D004259:DNA-Directed DNA Polymerase; D003849:Deoxyguanosine