Selection of a pure cerebellar granule cell culture by kainate treatment.
Chronic exposure of dissociated cerebellar cultures to 50 microM kainate results in a complete loss of [3H]-GABA release which is a marker of GABAergic interneurons. No loss of granule cells was found and the glutamatergic nature of the granule cells appeared unaltered by the kainate treatment, since evoked release of [3H]-D-aspartate was maintained after kainate exposure. Glial cells in such cultures are virtually eliminated by treatment with an antimitotic such as cytarabine. In consequence a pure culture of cerebellar granule cells virtually free of stellate, basket and glial cells may be obtained by a combined chronic treatment of the cultures with kainate and cytarabine.
Drejer
J
J
CNS Division, Department of Cellular Neurochemistry, Soeborg, Denmark.
Schousboe
A
A
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D005971:Glutamates / Q000502:physiology; D007608:Kainic Acid / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005971:Glutamates; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D007608:Kainic Acid