Isolation and characterization of two immunoregulatory factors--interleukin 2 inhibitors.
Supernatants of cultured gibbon T lymphoma cells MLA144 were shown to contain two suppressive factors (IF50 and IF80) isolated by ammonium sulfate precipitation, ion-exchange chromatography and gel filtration. Both factors had similar activities: inhibition of IL 2 production by human peripheral blood mononuclear cells, inhibition of IL 2 responsiveness of human lymphoblasts and mouse thymocytes, and inhibition of proliferation of Con A-stimulated mouse splenocytes. However, they did not affect E receptors. Fc receptors or phagocytosis. The physico-chemical properties of these two factors were also different. It is of special interest to note that the MLA144 cell line which spontaneously secretes IL 2 also generates IL 2 inhibitors. This offers an experimental model for investigation of the intracellular immunoregulatory mechanism of IL 2 expression.
Wu
Y Y
YY
Zhang
J
J
Zhang
Y H
YH
D000818:Animals; D002460:Cell Line; D007376:Interleukin-2 / Q000037:antagonists & inhibitors*; D008222:Lymphokines / Q000378:metabolism / Q000494:pharmacology*; D008223:Lymphoma / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL
D007376:Interleukin-2; D008222:Lymphokines; C046147:interleukin 2 inhibitor