The effects of maternal caffeine intake during pregnancy on mineral contents of fetal rat bone.
Various levels of maternal caffeine ingestion during pregnancy were investigated to determine whether caffeine will affect the mineral contents of the growing bones of fetal rats. On day 8 of gestation, rat dams were fed with a 20% protein diet supplemented with 0.5 mg, 1 mg, or 2 mg caffeine/100 g of dams body weight as an experimental group and the same without caffeine as a control until day 22 of gestation. Fetuses were removed by cesarean section on day 22 and mandibular bones were removed to study the mineral contents of calcium, phosphorus, magnesium, and zinc. Although the mandible weighed more in the 0.5-mg caffeine group as compared to the controls, an additional increase of caffeine resulted in a decrease in weight. All calcium, magnesium, and zinc contents per bone decreased in the 1- and 2-mg caffeine groups as compared to either controls or 0.5-mg caffeine group, whereas phosphorus stayed relatively constant regardless of the different levels of caffeine intake. When data are expressed as per gram of bone tissue, most of the mineral contents among the groups disappeared suggesting that normal growth and development of the fetal bone are impaired as a results of maternal caffeine intake. Caffeine intake during gestation in the present study indicates that different levels of caffeine intake may exert not only different effects on mineral contents in bone development, but also affect the growth of the fetal bone.
Nakamoto
T
T
Dept. of Physiology, Louisiana State University Medical Center, New Orleans 70119.
Grant
S
S
Yazdani
M
M
D000818:Animals; D002110:Caffeine / Q000008:administration & dosage / Q000494:pharmacology*; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D008334:Mandible / Q000032:analysis / Q000187:drug effects / Q000196:embryology*; D008903:Minerals / Q000032:analysis*; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D008903:Minerals; D002110:Caffeine