Significance of alteration in biodistribution of labeled lymphocytes exposed to stannous ion.
The biodistribution patterns of 99mTc (99mTc-lymph) and 111In-lymphocytes with [111In-(Sn)-lymph] or without (111In-lymph) stannous ion treatment was compared in Lewis rats. Syngeneic lymphocytes were labeled with either 125 microCi (4.63 MBq) 99mTc or 5 microCi (185 kBq) 111In per 2 x 10(7) cells. Mean labeling efficiency for 99mTc and 111In was 68.61% +/- 3.90% (SEM) and 87.22% +/- 2.01% (SEM) respectively. 99mTc-lymph (n = 4), 111In-lymph (n = 6) and 111In-(Sn)-lymph (n = 6) rats received 2 x 10(7) cells and were killed 18 h later. While 99mTc-lymph demonstrated significantly less localization in spleen, lymph nodes, and blood (P(F) less than 0.01) as compared with 111In-lymph, 111In-(Sn)lymph also demonstrated a significant difference (P[F]= 0.0001) in lymph node accumulation when compared to 111In-lymph. As the activity levels utilized are not associated with cell radiation damage, these alterations in biodistribution do not reflect viability or chromosomal damage, but appear related to stannous ion exposure.
Tatum
J L
JL
Department of Radiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0001.
Hirsch
J I
JI
Burke
T S
TS
Fratkin
M J
MJ
D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D007205:Indium Radioisotopes; D007477:Ions; D007553:Isotope Labeling; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011917:Rats, Inbred Lew; D013667:Technetium; D014001:Tin / Q000494:pharmacology*
D007205:Indium Radioisotopes; D007477:Ions; D013667:Technetium; D014001:Tin