title: Ovine opsonins for Staphylococcus aureus cell wall and pseudocapsule.
abstract: Blood serum, milk whey and purified IgG1 and IgG2 antibody preparations from sheep immunised with live or killed Staphylococcus aureus vaccines were assayed for opsonising activity against S aureus using ovine mammary neutrophils as effector cells. Serum had much higher levels of opsonins than whey; whey did not inhibit the potent opsonising activity of hyperimmune serum. IgG1 antibodies against cell wall antigens of in vitro-grown S aureus and pseudocapsular antigens of in vivo-grown S aureus had low opsonising activity. IgG2 antibody against in vitro-grown S aureus surface antigens had significantly greater opsonising activity than IgG1 of this specificity. The most powerful opsonising activity was associated with IgG2 antibody against the pseudo-capsular antigens produced by S aureus grown under in vivo, or simulated in vivo cultural conditions.
authors: CSIRO, Division of Animal Health, Armidale, New South Wales, Australia.
authors: D L
authors: DL
authors: Watson
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D007785:Lactose / Q000276:immunology; D008892:Milk / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis; D012756:Sheep / Q000276:immunology*; D013209:Staphylococcal Vaccines / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D007074:Immunoglobulin G; D009895:Opsonin Proteins; D013209:Staphylococcal Vaccines; D007785:Lactose