Cloning and sequence analysis of cDNA for precursor of a crustacean hyperglycemic hormone.
Crustacean hyperglycemic hormone (CHH) from Carcinus maenas, a 72 amino acid neuropeptide, originates in neurosecretory perikarya in the eyestalk ganglia. Poly (A)RNA was isolated from these perikarya and a cDNA library was prepared. Screening of 20,000 clones with a 26-mer oligonucleotide, corresponding to a partial sequence of CHH, yielded one positive clone with an insert of approximately 2,000 bp, which contained the complete coding sequence for a pre-pro CHH. This precursor consists of a putative 26 amino acid signal sequence, a 38 amino acid peptide of unknown function (Peptide C), and the CHH sequence at the carboxyl end. The CHH-sequence is flanked N-terminally by a Lys-Arg cleavage site and C-terminally by the tetrapeptide Gly-Arg-Lys-Lys which is followed by the stop codon.
Weidemann
W
W
Institut für Zoophysiologie der Rheinischen Friedrich-Wilhelms-Universität, Bonn, FRG.
Gromoll
J
J
Keller
R
R
D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015723:Gene Library; D007447:Invertebrate Hormones; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*
D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D011498:Protein Precursors; C015947:hyperglycemic hormone, crustacean; C061766:hyperglycemic hormone precursor, crustacean; D004247:DNA