Expression of protein kinase C subspecies in human leukemia-lymphoma cell lines.
Expression of protein kinase C (PKC) subspecies was studied in various human leukemia-lymphoma cell lines. The PKC in most cell lines examined was resolved into two major fractions corresponding to type II (beta-sequence) and type III (alpha-sequence) PKC of the rat brain. The amounts of these two subspecies greatly varied among the cell lines. Type I PKC (gamma-sequence) was expressed in none of the cell lines tested, but PKCs with undefined structures were frequently detected. The differential co-expression of several PKC subspecies is presumably related to the state of cell differentiation.
Sawamura
S
S
Department of Biochemistry, Kobe University School of Medicine, Japan.
Ase
K
K
Berry
N
N
Kikkawa
U
U
McCaffrey
P G
PG
Minowada
J
J
Nishizuka
Y
Y
D000818:Animals; D001402:B-Lymphocytes; D001921:Brain / Q000201:enzymology; D002845:Chromatography; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007938:Leukemia / Q000201:enzymology*; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology; D015479:Leukemia, Myelomonocytic, Acute / Q000201:enzymology; D008223:Lymphoma / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured
D011493:Protein Kinase C