DPH lifetime distributions in vesicles containing phospholipid hydroperoxides.
Multifrequency phase fluorometry was used to determine the lifetime distributions of 1,6 diphenyl-1,3,5-hexatriene in 1-palmitoyl-2 linoleoyl phosphatidylcholine small unilamellar vesicles containing 2% incorporation of phospholipid hydroperoxides. A biexponential decay was observed in both vesicle preparations over a temperature range of 5 to 35 degrees C. Vesicles containing phospholipid hydroperoxides showed an overall longer lifetime as well as a greater distribution in width. These findings suggest that phospholipid hydroperoxides create structural heterogeneity in membrane structure.
Wratten
M L
ML
Department of Pathology and Toxicology, University of Southern California, Los Angeles 90033.
Gratton
E
E
van de Ven
M
M
Sevanian
A
A
D002851:Chromatography, High Pressure Liquid; D004161:Diphenylhexatriene / Q000493:pharmacokinetics*; D015227:Lipid Peroxidation; D008563:Membrane Lipids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011090:Polyenes / Q000493:pharmacokinetics*; D013050:Spectrometry, Fluorescence
D008563:Membrane Lipids; D010743:Phospholipids; D011090:Polyenes; D004161:Diphenylhexatriene