title: Partial purification of human urinary megakaryocyte colony-stimulating factor.
abstract: Urinary extracts from patients with aplastic anemia are known to promote murine megakaryocytopoiesis. In this report, we show a simple method for the partial purification of megakaryocyte colony-stimulating factor from human urine. A four-step purification procedure, which included ethanol precipitation, CM Affi-Gel Blue chromatography, wheat germ agglutinin-Sepharose chromatography and high-resolution hydroxyapatite chromatography, resulted in an about 430- to 630-fold increase of specific activity. The final fractions were still contaminated with erythropoietin, but the contaminated content of erythropoietin was not enough to stimulate mouse megakaryocytopoiesis in our culture system. We also demonstrate that human urinary extracts stimulated human megakaryocyte colony formation.
authors: Third Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
authors: K
authors: K
authors: Ogata
authors: S
authors: S
authors: Kuriya
authors: K
authors: K
authors: Dan
authors: T
authors: T
authors: Nomura
mesh_terms: D000741:Anemia, Aplastic / Q000652:urine*; D011232:Chemical Precipitation; D002845:Chromatography; D002847:Chromatography, Agarose; D003115:Colony-Stimulating Factors / Q000302:isolation & purification / Q000494:pharmacology / Q000652:urine*; D058851:GPI-Linked Proteins; D006801:Humans; D006882:Hydroxyapatites; D008533:Megakaryocytes / Q000166:cytology*; D008562:Membrane Glycoproteins; D000090204:Mesothelin; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology; D011507:Proteinuria; D014909:Wheat Germ Agglutinins
chemical_list: D003115:Colony-Stimulating Factors; D058851:GPI-Linked Proteins; D006882:Hydroxyapatites; D008562:Membrane Glycoproteins; D011506:Proteins; D014909:Wheat Germ Agglutinins; D000090204:Mesothelin