Characterization of the adenovirus 2 virion protein, mu.
Adenovirus 2 virions contain a small, highly basic protein known as mu (mu). Partial sequence analysis of mu labeled with radioactive amino acids showed that it is derived from an 11-kDa virion precursor protein, L2-79R. Amino acid analysis, direct microsequence analysis, time-of-flight mass spectrometer analysis, and chemical synthesis demonstrated that mu is the unmodified, 19 amino acid peptide obtained from the 79-residue precursor by adenovirus-encoded proteinase-mediated cleavage after glycine31 and glycine50. Mu bound tightly to DNA and was located in the virion core. In vitro, mu could precipitate DNA fragments, suggesting that it may have a role in viral chromosome condensation.
Anderson
C W
CW
Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
Young
M E
ME
Flint
S J
SJ
D000260:Adenoviruses, Human / Q000032:analysis* / Q000235:genetics; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000138:chemical synthesis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000032:analysis / Q000235:genetics; D014758:Viral Core Proteins / Q000032:analysis* / Q000138:chemical synthesis / Q000235:genetics; D014771:Virion / Q000032:analysis* / Q000235:genetics
D000596:Amino Acids; D004268:DNA-Binding Proteins; D011498:Protein Precursors; D014758:Viral Core Proteins; C061289:virion protein mu, adenovirus