Developmentally regulated expression of the neural cell adhesion molecule (NCAM) by mouse thymocytes.
The expression of the neural cell adhesion molecule (NCAM) has been investigated during thymus ontogeny. NCAM mRNA was readily detectable at day 19 of gestation, the youngest age studied. Its level declined after birth to become undetectable at 3 weeks of age. Cell surface expression of NCAM protein was detected on 14% of day 15 fetal thymocytes and peaked during the perinatal period, when around 40% of the thymocytes expressed low to medium levels of NCAM. At postnatal day 2, the vast majority of the NCAM+ cells were also CD4+ and CD8+. At embryonic day 15, NCAM appeared also to be expressed by CD4- thymocytes since 14% of the cells were already NCAM+ whereas CD4 was virtually undetectable. In frozen section of the newborn thymus, surface staining for NCAM was present on a subpopulation of cells in the cortex, rare in the medulla and absent from the sub-capsular area. In conjunction with other cell adhesion molecules, NCAM could play a role in cell interactions during thymic development.
Brunet
J F
JF
Centre d'Immunologie, INSERM-CNRS de Marseille-Luminy, France.
Hirsch
M R
MR
Naquet
P
P
Uberla
K
K
Diamantstein
T
T
Lipinski
M
M
Goridis
C
C
D000367:Age Factors; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D015815:Cell Adhesion Molecules; D005333:Fetus / Q000032:analysis; D005434:Flow Cytometry; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000032:analysis*
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D012333:RNA, Messenger