Intracellular accumulation of cholesteryl esters suppresses production of lipopolysaccharide-induced interleukin 1 by rat peritoneal macrophages.
Interleukin 1 (IL-1) is a major cytokine of macrophages secreted by several stimulants such as lipopolysaccharide (LPS). Macrophages are known to possess the scavenger receptor for acetylated low density lipoprotein (acetyl-LDL) and maleylated albumin. In the present study we determined effects of these ligands on LPS-induced IL-1 production by rat peritoneal macrophages. These ligands themselves did not induce IL-1 production. However, upon short incubation with acetyl-LDL, LPS-induced IL-1 production was significantly suppressed. The extent of the suppression was proportional to cellular cholesteryl esters. Thus, intracellular accumulation of cholesteryl esters might be responsible for suppression of LPS-induced IL-1 production.
Haga
Y
Y
Department of Biochemistry, Kumamoto University Medical School, Japan.
Takata
K
K
Araki
N
N
Sakamoto
K
K
Akagi
M
M
Morino
Y
Y
Horiuchi
S
S
D000418:Albumins / Q000494:pharmacology; D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism* / Q000502:physiology; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D007424:Intracellular Fluid / Q000378:metabolism*; D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010529:Peritoneal Cavity; D051381:Rats; D011919:Rats, Inbred Strains; D011973:Receptors, LDL / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000418:Albumins; D002788:Cholesterol Esters; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; C049877:maleylalbumin; D012710:Serum Albumin, Bovine