Correlation of structure with T cell responses of the three members of the HLA-DRw52 allelic series.
A third allele at the DRB3 locus, DRw52c, represents an intermediate sequence between DRw52a and DRw52b and may have arisen by a gene conversion-like event. The recognition of cells bearing these molecules by a number of alloreactive and antigen-specific DR-restricted T cell clones was analyzed. On the basis of a theoretical model of HLA class II structure, distinct amino acid clusters have been identified as motifs controlling TCR recognition. These are located both in the cleft and in the alpha-helical edge of the MHC class II recognition platform. Motifs shared between two alleles may restrict public T cell clones.
Gorski
J
J
Department of Microbiology, University of Geneva Medical School, Switzerland.
Irle
C
C
Mickelson
E M
EM
Sheehy
M J
MJ
Termijtelen
A
A
Ucla
C
C
Mach
B
B
D000483:Alleles; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D006684:HLA-DR Antigens / Q000235:genetics*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology*
D000596:Amino Acids; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C051104:HLA-DR52 antigen