[ELISA method using microplates developed with commercial antisera for measuring von Willebrand factor antigen].
The commercial availability of free and peroxidase-conjugated anti-FvW IgG fractions makes it possible to use ELISA methods for FvW: Ag determination in the routine blood coagulation laboratory. A highly sensitive microplate ELISA method was developed in our laboratory (capable of detecting 0.02% FvW: Ag), which proved to be reproducible, quick and not expensive. No significant differences were found between the results attained on different days in 4 control samples (variance analysis), variation coefficient about 12% being observed even for samples with only 11% FvW: Ag. The standard and control sample curves were parallel, which discarded a dilution effect in the assay. The correlation with Laurell's method was r = 0.889 (n = 34, p less than 0.001) and with ristocetin cofactor it was r = 0.677 (n = 19, p less than 0.005).
Jorquera
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Montoro
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Aznar
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Lerma
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Curats
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Bañuls
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Casaña
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D000941:Antigens / Q000032:analysis*; D001797:Blood Protein Electrophoresis; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007106:Immune Sera; D008832:Microchemistry; D011237:Predictive Value of Tests; D014841:von Willebrand Factor / Q000032:analysis
D000941:Antigens; D007106:Immune Sera; C029417:Von Willebrand antigen; D014841:von Willebrand Factor
Método ELISA en microplaca desarrollado con antisueros comerciales para medir el antígeno del factor von Willebrand.