Hydroxymethylglutaryl coenzyme A reductase activity of adult Hymenolepis diminuta.
The occurrence of hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase in adult Hymenolepis diminuta was demonstrated. This activity was negligible in the cestode's cytosolic fraction but was noted when the mitochondrial or microsomal fraction served as the enzyme source. The predominant localization of HMG-CoA reductase activity was with the microsomal fraction. This fraction did not contain appreciable mitochondrial contamination based on the distribution of marker enzymes. The enzymatic nature of HMG-CoA conversion to mevalonic acid by either fraction was apparent because the reaction was heat labile and responded linearly to time of assay and protein content. The enzymatic reduction of HMG-CoA absolutely required NADPH when either fraction was assayed. The lesser activity of the mitochondrial fraction was membrane-associated. The predominant localization of HMG-CoA reductase activity with microsomal membranes and its separation with the membranous component of the mitochondrial fraction suggest that mitochondrial activity reflects the presence of microsomal membranes. In its predominant localization and pyridine nucleotide requirement, the cestode's HMG-CoA reductase activity resembles that of mammalian systems. The finding of HMG-CoA reductase provides an enzymatic mechanism for the intermediate conversion of HMG-CoA to mevalonic acid that would be needed for acetate-dependent isoprenoid lipid synthesis by adult H. diminuta.
Fioravanti
C F
CF
Department of Biological Sciences, Bowling Green State University, Ohio 43403.
Kim
Y
Y
Batten
C L
CL
Weaver
J R
JR
D000818:Animals; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D006926:Hymenolepis / Q000201:enzymology*; D008798:Mevalonic Acid / Q000032:analysis / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D009249:NADP / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000032:analysis
D009249:NADP; D006903:Hydroxymethylglutaryl CoA Reductases; D013385:Succinate Dehydrogenase; D008798:Mevalonic Acid