title: Characterization of activation of a partially defective B cell subpopulation with immunocompetence restricted to IgM and IgA.
abstract: A B-cell subpopulation (BM-A cell) responding to an antigen with the production of IgM and IgA plaque-forming cells but not of IgG plaque-forming cells was isolated from neonatally bursectomized chickens and was examined for the mode of activation by B-cell mitogens. The BM-A cells did not elevate both glucose consumption and protein synthesis with the B-cell mitogens, in striking contrast to normal B cells. The BM-A cells were also not activated by an activator of protein kinase C, phorbol myristate acetate. Both anti-Ig and a calcium ionophore, A23187, however, primed the BM-A cells to increase intracellular free calcium ion as well as normal B cells. From these results it is conceived that the lack of protein kinase C activation may be responsible for the failure of activation of BM-A cells.
authors: Department of Animal Microbiology, College of Agriculture, University of Osaka.
authors: H
authors: H
authors: Nakaniwa
authors: T
authors: T
authors: Kajikawa
authors: Y
authors: Y
authors: Bito
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis / Q000276:immunology*; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D008934:Mitogens; D011494:Protein Kinases; D005947:Glucose; D002118:Calcium