title: Metabolism of 2,4-dinitrotoluene and 2,6-dinitrotoluene, and their dinitrobenzyl alcohols and dinitrobenzaldehydes by Wistar and Sprague-Dawley rat liver microsomal and cytosol fractions.
abstract: The metabolism of 2,4-dinitrotoluene (2,4-DNT), 2,4-dinitrobenzyl alcohol (2,4-DNB), 2,4-dinitrobenzaldehyde (2,4-DNBAl), 2,6-DNT, 2,6-DNB and 2,6-DNBAl in the microsomal and cytosol fractions prepared from unfortified male Wistar and male Sprague-Dawley (S.D.) rat livers was investigated. Data obtained by high-performance liquid chromatography (HPLC) indicated that the products of dinitrotoluenes (2,4-DNT and 2,6-DNT), dinitrobenzyl alcohols (2,4-DNB and 2,6-DNB), and dinitrobenzaldehydes (2,4-DNBAl and 2,6-DNBAl) in the microsomal and cytosol preparations containing nicotinamide adenine dinucleotide phosphate (NAD(P] and reduced NAD(P)(NAD(P)H) were dinitrobenzyl alcohols (2,4-DNB and 2,6-DNB), dinitrobenzaldehydes (2,4-DNBAl and 2,6-DNBAl), and dinitrobenzoic acids (2,4-DNBA and 2,6-DNBA), and dinitrobenzyl alcohols (2,4-DNB and 2,6-DNB), respectively. From these results, it was concluded that the dinitrobenzaldehydes (2,4-DNBAl and 2,6-DNBAl) were intermediates in the oxidations of dinitrobenzyl alchols (2,4-DNB and 2,6-DNB) to dinitrobenzoic acids (2,4-DNBA and 2,6-DNBA), and that the oxidations of dinitrobenzyl alcohols (2,4-DNB and 2,6-DNB) to dinitrobenzaldehydes (2,4-DNBAl and 2,6-DNBAl) and the reductions of dinitrobenzaldehydes to dinitrobenzyl alcohols (2,4-DNB and 2,6-DNB) were reversible.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: M
authors: M
authors: Mori
authors: T
authors: T
authors: Kawajiri
authors: M
authors: M
authors: Sayama
authors: T
authors: T
authors: Miyahara
authors: H
authors: H
authors: Kozuka
mesh_terms: D000818:Animals; D001547:Benzaldehydes / Q000032:analysis; D001592:Benzyl Alcohols / Q000032:analysis; D003600:Cytosol / Q000378:metabolism*; D004136:Dinitrobenzenes / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009578:Nitrobenzenes / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001547:Benzaldehydes; D001592:Benzyl Alcohols; D004136:Dinitrobenzenes; D009578:Nitrobenzenes; C016403:2,4-dinitrotoluene; C023514:2,6-dinitrotoluene