Fetal pathology produced by ethylene oxide treatment of the murine zygote.
Exposure of female mice to ethylene oxide by inhalation 1 or 6 h after mating produced not only multitemporal death of conceptuses but also high rates of abnormalities among surviving fetuses. In contrast, only marginal effects were observed when females were exposed 9 or 25 h after mating. The abnormalities found among 17 day gestation live fetuses were predominated by hydrops and eye defects, which, together, constitute 54% of all anomalies. Most of the remaining anomalies were distributed among 5 other types: small size, cleft palate, and cardiac, abdominal wall, or extremity and/or tail defects. In a follow-up study, the fetuses of females treated 6 h postmating were examined at 11-15 days gestation and the progression of fetal death and of malformations was studied. Results indicate that the expression of most fetal anomalies does not become apparent until late in gestation. Several of these induced anomalies are similar to common human sporadic birth defects. This new class of experimentally induced fetal anomalies provides a new avenue for investigating zygotic biology and a system for studying the progression of aberrant development.
Rutledge
J C
JC
Children's Medical Center, University of Texas Southwestern Medical Center, Dallas 75235.
Generoso
W M
WM
D000818:Animals; D002970:Cleavage Stage, Ovum / Q000187:drug effects; D015331:Cohort Studies; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D005027:Ethylene Oxide / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000473:pathology*; D005500:Follow-Up Studies; D005865:Gestational Age; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009153:Mutagens; D011247:Pregnancy; D011897:Random Allocation; D015053:Zygote / Q000187:drug effects*
D009153:Mutagens; D004247:DNA; D005027:Ethylene Oxide