Localization of enkephalinase mRNA in rat brain by in situ hybridization: comparison with immunohistochemical localization of the protein.
The messenger RNA (mRNA) encoding enkephalinase (EC. 3.4.24.11; neutral endopeptidase) has been localized in rat brain by in situ hybridization using 35S- or 32P-labelled cRNA probes. Hybridization was observed only in few brain areas, and was particularly strong in the striatum, olfactory bulb and pontine nuclei. The enkephalinase protein was also localized in brain sections using a radiolabelled monoclonal antibody. While some brain regions contained both the mRNA and its translation product, others, including in particular the substantia nigra, were rich in enkephalinase but did not contain any detectable amount of enkephalinase mRNA. Enkephalinase mRNA-containing cells could be identified in regions containing neurons known to project to the substantia nigra. The discrepancy between the mRNA and the protein labelling is likely to reflect the fact that the mRNA is exclusively located within the soma of the cells while the translated protein may be found anywhere along the axonal processes.
Wilcox
J N
JN
Department of Molecular Biology, Genetech, Inc., South San Francisco, CA 94080.
Pollard
H
H
Moreau
J
J
Schwartz
J C
JC
Malfroy
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000201:enzymology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D015260:Neprilysin / Q000235:genetics* / Q000276:immunology; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D015347:RNA Probes; D012333:RNA, Messenger; D015260:Neprilysin