title: Evaluation of the polyethylene glycol-indirect antiglobulin test for routine compatibility testing.
abstract: All specimens received in the blood bank over a 5-month period for crossmatch or group and screen requests were tested in parallel by a polyethylene glycol-indirect antiglobulin test (PEG-IAT) and a low-ionic-strength saline (LISS)-IAT. The sera of 41 of 1471 patients had reactions, with 50 antibodies being detected. Ten antibodies reacted only on the PEG-IAT and 14 only by the LISS-IAT; the remaining 26 antibodies were detected by both methods. Of the antibodies that reacted only by the LISS-IAT, one (anti-Jka) was considered clinically significant, whereas five of the antibodies that reacted only by the PEG-IAT (1 anti-c, 2-Fya, 1-Jkb, and 1-S) were considered significant. Two antibodies of questionable clinical significance were detected only by the PEG-IAT. In 97 percent of the sera tested, no reaction was detected by either method. The PEG-IAT is an acceptable technique for routine compatibility testing.
authors: Regional Laboratory Services, Royal Columbian Hospital, New Westminster, British Columbia, Canada.
authors: J L
authors: JL
authors: Slater
authors: D J
authors: DJ
authors: Griswold
authors: L S
authors: LS
authors: Wojtyniak
authors: M J
authors: MJ
authors: Reisling
mesh_terms: D001788:Blood Grouping and Crossmatching / Q000379:methods*; D003298:Coombs Test; D006801:Humans; D011092:Polyethylene Glycols
chemical_list: D011092:Polyethylene Glycols