Isolation and characterization of an antitumor active agar-type polysaccharide of Gracilaria dominguensis.
Cold water extraction of the red alga Gracilaria dominguensis, followed by cetyltrimethylammonium bromide fractionation, gave a highly sulfated, agar-type polysaccharide which inhibited the transplantation of Ehrlich ascites carcinoma in mice. The structure of the polysaccharide has been investigated by methylation analysis, and 1H- and 13C-n.m.r. spectroscopy, and was shown to be mainly composed of alternating (1----3)-linked beta-D-galactopyranosyl 6-sulfate and (1----4)-linked 3,6-anhydro-alpha-L-galactopyranosyl residues.
Fernández
L E
LE
Departamento de Bioquímica, Instituto Nacional de Oncología y Radiobiología, Havana, Cuba.
Valiente
O G
OG
Mainardi
V
V
Bello
J L
JL
Vélez
H
H
Rosado
A
A
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000627:therapeutic use; D002236:Carbohydrate Conformation; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy; D002845:Chromatography; D005690:Galactose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D051379:Mice; D015394:Molecular Structure; D011134:Polysaccharides / Q000302:isolation & purification* / Q000627:therapeutic use; D000461:Rhodophyta / Q000032:analysis*; D014461:Ultracentrifugation
D000970:Antineoplastic Agents; D011134:Polysaccharides; D005690:Galactose