[Determination of amyloid P component in blood by an immunoenzyme method].
An ELISA procedure was developed for measuring serum amyloid P-component (SAP). The assay is based on our finding of binding AP to polystyrene microtiter plates. The amount of SAP is determined by concurrent ELISA, wells being sequentially incubated with rabbit anti-AP antiserum and goat anti-rabbit antiserum, conjugated with peroxidase. The limit of sensitivity of the assay is 0.5 micrograms/ml. When applied to the screening of patients plasma, elevation of SAP concentration in patients with rheumatoid arthritis and amyloidosis were found.
Bannikova
E M
EM
Nasonov
E L
EL
Tishchenko
V A
VA
Nagornaia
L V
LV
Sura
V V
VV
D000686:Amyloidosis / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D000683:Serum Amyloid P-Component / Q000032:analysis*
D000683:Serum Amyloid P-Component
Opredelenie amiloidnogo P-komponenta v plazme krovi s pomoshch'iu immunofermentnogo metoda.