Effect of phosphatidylcholine on the alpha-lactalbumin-induced fusion of vesicles.
Previous studies on alpha-lactalbumin induced fusion of phosphatidylserine/phosphatidylethanolamine vesicles are extended to vesicles composed of various combinations of phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and cardiolipin. It was found that inclusion of phosphatidylcholine in the vesicles results in a depression of fusion. This depression of fusion appears to be caused by a reduction in the amount of irreversibly bound alpha-lactalbumin to vesicles containing phosphatidylcholine. It is suggested that in this system fusion is dependent upon the extent by which a particular protein segment penetrates the bilayer.
Park
B S
BS
Department of Science Education, Dankook University, Seoul, Korea.
Kim
J
J
Kim
U H
UH
Kim
H
H
D006863:Hydrogen-Ion Concentration; D007768:Lactalbumin / Q000378:metabolism / Q000494:pharmacology*; D008081:Liposomes / Q000378:metabolism*; D008561:Membrane Fusion / Q000187:drug effects*; D010713:Phosphatidylcholines / Q000378:metabolism*; D011485:Protein Binding; D014357:Trypsin
D008081:Liposomes; D010713:Phosphatidylcholines; D007768:Lactalbumin; D014357:Trypsin