Hydrolysis of retinyl palmitate by enzymes of rat pancreas and liver. Differentiation of bile salt-dependent and bile salt-independent, neutral retinyl ester hydrolases in rat liver.
Previous studies have demonstrated that homogenates of the livers of rats contain a neutral retinyl ester hydrolase activity that requires millimolar concentrations of bile salts for maximal in vitro activity. The enzymatic properties of this neutral, bile salt-dependent retinyl ester hydrolase activity in liver homogenates are nearly identical to those observed in the present report for the in vitro hydrolysis of retinyl palmitate by purified rat pancreatic cholesteryl ester hydrolase (EC 3.1.1.13). Moreover, anti-rat pancreatic cholesteryl ester hydrolase IgG completely inhibits the bile salt-dependent retinyl ester hydrolase activity of rat liver homogenates whereas normal rabbit IgG does not. We also show that liver homogenates contain a neutral, bile salt-independent retinyl ester hydrolase activity that differs from the bile salt-dependent activity in that 1) its absolute activity does not vary markedly among individual rats, 2) it is not inhibited by antibodies to pancreatic cholesteryl ester hydrolase, and 3) it is localized in the microsomal fraction of liver homogenates. Subfractionation of microsomes demonstrates that the neutral, bile salt-independent retinyl ester hydrolase activity is associated with liver cell plasma membranes and thus may play a role in the hydrolysis of retinyl esters delivered to the liver by chylomicron remnants.
Harrison
E H
EH
Department of Physiology and Biochemistry, Medical College of Pennsylvania, Philadelphia 19129.
Gad
M Z
MZ
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004224:Diterpenes; D006868:Hydrolysis; D007074:Immunoglobulin G; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D000084562:Retinyl Esters; D002787:Sterol Esterase / Q000276:immunology / Q000378:metabolism; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000378:metabolism
D001647:Bile Acids and Salts; D004224:Diterpenes; D007074:Immunoglobulin G; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate; D002265:Carboxylic Ester Hydrolases; C022523:retinyl esterase; D002787:Sterol Esterase