title: Detection of 2-keto-3-deoxyoctonate in endotoxins isolated from six reference strains of the Bacteroides fragilis group.
abstract: 1. Endotoxins isolated from six serotype specific reference strains of the Bacteroides fragilis group were dephosphorylated by treatment with aqueous 50% hydrofluoric acid. 2. Mild acidic hydrolysis of the dephosphorylated endotoxins released 2-keto-3-deoxyaldonic acid, the presence of which was demonstrated by the colorimetric thiobarbituric acid assay (TBA). 3. Thin layer chromatography of the dephosphorylated lipopolysaccharide of B. fragilis IPL E 323 (serotype E2), after acidic hydrolysis, revealed a TBA-positive substance with the same Rf-value as authentical 2-keto-3-deoxyoctolusonic acid (KDO). 4. Quantification of 2-keto-3-deoxyoctonate-in the lipopolysaccharide of B. fragilis IPL E 323 by means of the TBA resulted in a KDO content of 15 nM mg-1 lipopolysaccharide.
authors: Department of Obstetrics and Gynecology, Erasmus University, Rotterdam, The Netherlands.
authors: I
authors: I
authors: Beckmann
authors: H G
authors: HG
authors: van Eijk
authors: F
authors: F
authors: Meisel-Mikołajczyk
authors: H C
authors: HC
authors: Wallenburg
mesh_terms: D001441:Bacteroides fragilis / Q000032:analysis*; D002855:Chromatography, Thin Layer; D004731:Endotoxins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000302:isolation & purification; D010766:Phosphorylation; D013400:Sugar Acids / Q000032:analysis*; D013858:Thiobarbiturates
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D013400:Sugar Acids; D013858:Thiobarbiturates; C002532:2-keto-3-deoxyoctonate; C029684:thiobarbituric acid