title: Endothelial cell subpopulations in vitro: cell volume, cell cycle, and radiosensitivity.
abstract: Vascular endothelial cells (EC) are important clinical targets of radiation and other forms of free radical/oxidant stresses. In this study, we found that the extent of endothelial damage may be determined by the different cytotoxic responses of EC subpopulations. The following characteristics of EC subpopulations were examined: 1) cell volume; 2) cell cycle position; and 3) cytotoxic indexes for both acute cell survival and proliferative capacity after irradiation (137Cs, gamma, 0-10 Gy). EC cultured from bovine aortas were separated by centrifugal elutriation into subpopulations of different cell volumes. Through flow cytometry, we found that cell volume was related to the cell cycle phase distribution. The smallest EC were distributed in G1 phase and the larger cells were distributed in either early S, middle S, or late S + G2M phases. Cell cycle phase at the time of irradiation was not associated with acute cell loss. However, distribution in the cell cycle did relate to cell survival based on proliferative capacity (P less than 0.01). The order of increasing radioresistance was cells in G1 (D0 = 110 cGy), early S (135 cGy), middle S (145 cGy), and late S + G2M phases (180 cGy). These findings 1) suggest an age-related response to radiation in a nonmalignant differentiated cell type and 2) demonstrate EC subpopulations in culture.
authors: Department of Medicine, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois.
authors: D B
authors: DB
authors: Rubin
authors: E A
authors: EA
authors: Drab
authors: K D
authors: KD
authors: Bauer
mesh_terms: D000818:Animals; D002417:Cattle; D002453:Cell Cycle / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004730:Endothelium, Vascular / Q000166:cytology* / Q000528:radiation effects; D005434:Flow Cytometry; D007399:Interphase / Q000528:radiation effects; D008297:Male