Vicia villosa agglutinin separates freshly isolated Peyer's Patch T cells into interleukin 5- or interleukin 2-producing subsets.
Murine L3T4 T cells freshly isolated from Peyer's Patch were fractionated based on differential adherence to Vicia villosa agglutinin (VVA). VVA adherent cells secreted IL-5, but not IL-2, after stimulation with Con A and IL-1. In striking contrast, VVA nonadherent PP L3T4 T cells secreted IL-2, but not IL-5, under the same conditions. In addition, supernatants from VVA adherent, but not from VVA nonadherent cells cultures, enhanced IgA secretion by LPS-stimulated splenic B cells to the same extent as purified IL-5. Thus, IL-5-producing T cells are present in PP in situ and may play an important role in the development of mucosal immunity. Further, differential adherence to VVA can be used to separate T cell populations that preferentially secrete IL-5 or IL-2.
Schoenbeck
S
S
Department of Medicine, University of California, San Diego, La Jolla 92037.
Hammen
M J
MJ
Kagnoff
M F
MF
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D007376:Interleukin-2 / Q000096:biosynthesis*; D015848:Interleukin-5; D007378:Interleukins / Q000096:biosynthesis*; D037102:Lectins / Q000494:pharmacology*; D051379:Mice; D010581:Peyer's Patches / Q000166:cytology; D037121:Plant Lectins; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism
D007376:Interleukin-2; D015848:Interleukin-5; D007378:Interleukins; D037102:Lectins; D037121:Plant Lectins; C042242:Vicia lectins