IL-1 and PGE2 productions by the regional lymph node cells from DNFB-sensitized mice.
Interleukin 1 (IL-1) and Prostaglandin E2 (PGE2) are mainly produced by macrophage/monocytes. In this experiment, we investigated the immunological role of macrophage/monocytes in the lymph nodes (LNs) by measuring IL-1 and PGE2 productions of regional LN cells from dinitrofluorobenzene (DNFB)-sensitized and -tolerant mice. LN cells from sensitized mice produced IL-1 in remarkable amounts after in vitro antigen stimulation. On the other hand, the LN cells from tolerant mice failed to produce IL-1 or PGE2. These data indicate that, by modifying cytokine production, macrophage/monocytes in the regional LNs play a key role in the pathomechanism of contact hypersensitivity.
Natsuaki
M
M
Higasa
M
M
Sagami
S
S
Shinka
S
S
D000818:Animals; D003877:Dermatitis, Contact / Q000378:metabolism*; D004139:Dinitrofluorobenzene / Q000276:immunology*; D015232:Dinoprostone / Q000032:analysis / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009578:Nitrobenzenes / Q000276:immunology*
D007375:Interleukin-1; D009578:Nitrobenzenes; D004139:Dinitrofluorobenzene; D015232:Dinoprostone