title: Inhibition of complement-factor-5a-induced inflammatory reactions by prostaglandin E2 in experimental meningitis.
abstract: The possible role of complement factor 5a (C5a) and prostaglandin E2 (PGE2) in cerebrospinal fluid (CSF) pleocytosis and protein accumulation was assessed in a rabbit model of meningitis. Intracisternally administered C5a caused a rapid, early influx of leukocytes into CSF that peaked at 1 h after injection; by 6 h, cell counts were slightly higher than those in controls. Administration of PGE2 or saline did not induce detectable CSF leukocytosis. Coadministration of PGE2 with C5a decreased CSF leukocytosis in a dose-related fashion. Protein concentration increased 30 min after administration of C5a, peaked after 1 h, and remained elevated for 6 h. PGE2 caused a dose-related increase in protein content after 2 h, whereas coadministration caused an inversely dose-related inhibition of the C5a-induced protein influx into CSF. These data suggest that PGE2 in the subarachnoid space exerts an inhibitory action on the C5a-mediated response that is probably not related to its direct effects on protein extravasation.
authors: Pharmaceutical Research Laboratories, CIBA-GEIGY Ltd., Basel, Switzerland.
authors: J L
authors: JL
authors: Kadurugamuwa
authors: B
authors: B
authors: Hengstler
authors: M A
authors: MA
authors: Bray
authors: O
authors: O
authors: Zak
mesh_terms: D000818:Animals; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis; D015936:Complement C5a / Q000008:administration & dosage / Q000134:cerebrospinal fluid* / Q000276:immunology; D015232:Dinoprostone / Q000008:administration & dosage / Q000134:cerebrospinal fluid / Q000276:immunology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007958:Leukocyte Count; D007964:Leukocytosis / Q000134:cerebrospinal fluid; D008297:Male; D008586:Meningitis, Pneumococcal / Q000276:immunology*; D011817:Rabbits
chemical_list: D002556:Cerebrospinal Fluid Proteins; D015936:Complement C5a; D015232:Dinoprostone