[Selective induction of dog esophageal carcinomas by the restricted oral administration of N-ethyl-N-'-nitro-N-nitrosoguanidine with a projecting spout].
In order to establish an effective method to induce selectively experimental dog esophageal carcinoma, we compared the restricted oral administration of N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) with projecting spout with the ad libitum oral administration of it. Five dogs were given a solution of ENNG at a concentration of 50mg/l with restricted oral administration with projecting spout for 52 weeks. In all of them, elevated type of esophageal lesions were endoscopically observed soon after the cessation of the ENNG administration. Histological examination revealed that besides the multiple squamous cell carcinomas of the esophagus, various degrees of dysplasias were seen. Two dogs had metastasizes to the regional lymph nodes and one dog had metastatic lesions in the lung. Gastric carcinomas were also seen in four dogs. Another five dogs were given ad libitum the same concentration of ENNG solution. Gastric carcinomas were induced in four dogs, but esophageal carcinomas were seen in small lesions in two dogs. The restricted oral administration of ENNG with projecting spout is a reliable method for the selective induction of esophageal carcinoma in dog.
Tsukahara
Y
Y
Department of Surgery, Osaka University, Japan.
Fujita
M
M
Ohmichi
M
M
Ishii
T
T
Taguchi
T
T
D000284:Administration, Oral; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage*; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced*; D004285:Dogs; D004938:Esophageal Neoplasms / Q000139:chemically induced*; D005260:Female; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000031:analogs & derivatives; D010212:Papilloma / Q000139:chemically induced; D013274:Stomach Neoplasms / Q000139:chemically induced
D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; C013525:ENNG