HydroLink gel electrophoresis (HLGE). III. High DNA loading capacity and recovery of dsDNA.
Novel polymers have been prepared for high performance electrophoretic separations of double-stranded DNA (dsDNA). These materials are part of a family of HydroLink high performance electrophoresis polymers. A comparison of the resolving capabilities of dsDNA HydroLink gels to agarose and polyacrylamide separations has been described in an accompanying paper. In this study, we demonstrate that dsDNA HydroLink gels possess ten times the loading capacity of comparable polyacrylamide or agarose gels without compromise to resolution or biological integrity of the separated DNA. A simplified procedure for recovery of separated components is also described.
Smith
C L
CL
Research and Development, AT Biochem, Inc., Malvern, PA 19355.
Ewing
C M
CM
Mellon
M T
MT
Shorr
R G
RG
Jain
T
T
D001482:Base Composition; D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005782:Gels; D007202:Indicators and Reagents; D008970:Molecular Weight; D011108:Polymers
D005782:Gels; D007202:Indicators and Reagents; D011108:Polymers; C061589:HydroLink; D004247:DNA