Preparation of white cell-depleted red cells for 42-day storage using an integral in-line filter.
A new blood pack system for the preparation of white cell-depleted red cells was studied. The system is a modified additive-solution quadruple-unit blood pack that incorporates a cellulose-acetate fiber depth filter in-line between the AS-3 additive bag and the CP2D collection bag. Mean +/- SD white cell removal from 156 units processed under standard production conditions was 97.7 +/- 2.7 percent; residual white cells were 1.1 +/- 1.0 x 10(8) per unit. Red cell loss was 10.0 +/- 1.0 percent (n = 43). Mean platelet removal was 80.9 percent from units from which platelet concentrates were not prepared (n = 47). Microaggregates did not form during storage, and hemolysis of filtered red cells was lower than that of unfiltered controls. Filtered AS-3 red cells stored for 42 days had a 51Cr survival of 80.1 +/- 5.7 percent (mean +/- SD) as compared with 78.9 +/- 6.2 percent for unfiltered controls (n = 17). This in-line filter system provides white cell-depleted, microaggregate-free red cells that can be stored for 42 days.
Davey
R J
RJ
Department of Transfusion Medicine, National Institutes of Health, Bethesda, Maryland.
Carmen
R A
RA
Simon
T L
TL
Nelson
E J
EJ
Leng
B S
BS
Chong
C
C
Garcez
R B
RB
Sohmer
P R
PR
D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation; D001803:Blood Transfusion / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003167:Complement Activation; D003176:Complement C3 / Q000378:metabolism; D015926:Complement C3a; D004903:Erythrocyte Aggregation; D004905:Erythrocyte Aging; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000502:physiology; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007962:Leukocytes
D003176:Complement C3; D015926:Complement C3a; D000255:Adenosine Triphosphate