title: [Isolation of high molecular weight eukaryotic DNA with the use of potassium acetate].
abstract: The simple technique for isolation of high-molecular eucaryotic DNA has been proposed. It includes cell or nuclei lysates by sodium dodecylsulfate in the presence of pronase, proteins precipitation by potassium acetate, DNA precipitation by ethanol. The DNA isolated by this technique is easily cleaved by restriction endonucleases and can be used for analysis of the unique genes by blot-hybridization. The yield of DNA is similar or somewhat higher than that in case of using the standard methods including phenol extraction or phenol-chloroform extraction.
authors: B P
authors: BP
authors: Sokolov
authors: V V
authors: VV
authors: Dzhemelinskiĭ
authors: V N
authors: VN
authors: Kalinin
mesh_terms: D000085:Acetates; D019342:Acetic Acid; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007202:Indicators and Reagents; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization
chemical_list: D000085:Acetates; D007202:Indicators and Reagents; D004247:DNA; D019342:Acetic Acid
vernacular_title: Vydelenie vysokomolekuliarnoĭ éukarioticheskoĭ DNK s ispol'zovaniem atsetata kaliia.