Correlation of hematologic recovery with CFU-GM content of autologous bone marrow grafts treated with 4-hydroperoxycyclophosphamide. Culture after cryopreservation.
We previously described an exponential correlation of the CFU-GM content of 4-hydroperoxycyclophosphamide-treated autologous bone marrow grafts and the rate of hematologic recovery of the recipients after transplantation. Those grafts were assayed before cryopreservation. We now describe a similar analysis based upon cultures of the thawed bone marrow. In a proportional hazards model, the CFU-GM content of the grafts sampled after thawing predicted time to achieving a peripheral blood count of 1 X 10(9)/l leukocytes, 0.5 X 10(9)/l granulocytes, 2% reticulocytes, and last platelet transfusion (p less than 0.001). Using linear regression analysis, the logarithm of the CFU-GM content/kg of recipient weight also was correlated with the time to achieving 1 X 10(9)/l leukocytes (r = -0.62), and 0.5 X 10(9)/l granulocytes (r = -0.66). The hazard ratios (Cox model) and regression coefficients (linear regression model) for the equations calculated from the post-thaw cultures were usually smaller than the corresponding coefficients based upon the pre-freeze cultures, suggesting that pre-freeze cultures may be more accurate predictors of aplasia duration. However, patients with poor post-freeze survival of CFU-GM were more likely to experience delayed engraftment. Therefore, the correlation of post-thaw cultures with engraftment may permit the analysis of cryopreservation techniques.
Rowley
S D
SD
Johns Hopkins Oncology Center, Baltimore, MD 21205.
Piantadosi
S
S
Santos
G W
GW
D000293:Adolescent; D000328:Adult; D001772:Blood Cell Count; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D016026:Bone Marrow Transplantation / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014182:Transplantation, Autologous
D003520:Cyclophosphamide; C011272:perfosfamide