Changes of linoleic acid metabolism and cellular phospholipid fatty acid composition in LLC-PK cells cultured at low magnesium concentrations.
LLC-PK cells grown on media containing normal (480 microM) or reduced magnesium levels (25, 6.3 or 2.5 microM) were used to study the effect of magnesium deficiency on linoleic acid metabolism and cellular membrane fatty acids. The fatty acid composition of the cellular phospholipids showed a significant decrease in 20:4(n-6) and 22:4(n-6) acids and a significant increase in 18:2(n-6), 18:3(n-6) and 20:3(n-6) fatty acids in magnesium-deficient cells compared to magnesium-sufficient cells. When [1-14C]linoleic acid was incubated with control (480 microM Mg2+) or magnesium deficient cells (2.6 microM Mg2+) the rate of tetraenoic acid synthesis (20:4(n-6) + 22:4(n-6) was significantly reduced in magnesium-deficient cells, indicating that the metabolic conversion of 18:2(n-6) to 20:4(n-6) is impaired in magnesium deficiency. This reduction in conversion may be due to the impairment of either the delta(5)- or the delta(6)-desaturase, or both. This study shows that magnesium deficiency perturbs essential fatty acid (EFA) metabolism and decreases the cellular membrane polyunsaturated fatty acid (PUFA) content. These alterations are likely to have adverse effects on cellular membrane properties and functions.
Mahfouz
M M
MM
Burnside Research Laboratory, University of Illinois, Urbana.
Smith
T L
TL
Kummerow
F A
FA
D000818:Animals; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D008275:Magnesium Deficiency / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D013552:Swine
D005227:Fatty Acids; D008041:Linoleic Acids; D010743:Phospholipids; D019787:Linoleic Acid