Mouse bone marrow-derived IL-3-dependent mast cells and autonomous sublines produce IL-6.
This study deals with the question of whether mouse bone marrow-derived mast cells are able to produce interleukin-6 (IL-6) in vitro. For this purpose, a panel of primary mast cell clones from limiting-dilution microcultures, of permanent IL-3-dependent mast cell lines and autonomous malignant sublines, was screened. All of these lines were found to produce growth factor activity for IL-6-dependent mouse hybridoma cells (7TD1), which could be completely neutralized by the monoclonal anti-IL-6-antibody 6B4. Transcriptional activity of the IL-6 gene was demonstrated in both IL-3-dependent mast cells and autonomous sublines using a mouse IL-6-specific cDNA probe.
Hültner
L
L
GSF-Institut für Experimentelle Hämatologie, Munich, FRG.
Szöts
H
H
Welle
M
M
Van Snick
J
J
Moeller
J
J
Dörmer
P
P
D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D007377:Interleukin-3 / Q000502:physiology*; D015850:Interleukin-6; D007378:Interleukins / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains
D007377:Interleukin-3; D015850:Interleukin-6; D007378:Interleukins