Prenatal diagnosis of beta-thalassaemia in Mediterranean populations by dot blot analysis with DNA amplification and allele specific oligonucleotide probes.
In this study, we describe a simple strategy to detect beta-thalassaemia mutations in prospective parents and to make prenatal diagnosis in pregnancies at risk in the Mediterranean population. Screening of prospective parents is carried out by dot blot analysis on enzymatically amplified DNA with a set of oligonucleotide probes complementary to the most common mutations in this population. Prenatal diagnosis is accomplished by the same procedure on enzymatically amplified amniocyte or trophoblast DNA. The main advantages of this procedure are the simplicity, sensitivity (0.05 micrograms of DNA), and rapidity (12-24 h). Further simplification is obtained by amplification of the DNA from crude amniotic cell lysate. The very low amount of fetal material necessary for this analysis eliminates the need to culture amniotic fluid cells and may decrease the fetal loss rate associated with trophoblast sampling. The number of specific DNA sequences obtained by the amplification procedure allowed us to use non-radioactive labelled oligonucleotide probes, which have several advantages compared to radioactive probes.
Ristaldi
M S
MS
Istituto di Ricerca sulle Talassemie e Anemie Mediterranee, CNR, Cagliari, Italy.
Pirastu
M
M
Rosatelli
C
C
Monni
G
G
Erlich
H
H
Saiki
R
R
Cao
A
A
D000483:Alleles; D000653:Amniotic Fluid / Q000032:analysis; D001483:Base Sequence; D002824:Chorionic Villi / Q000032:analysis; D004247:DNA / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007962:Leukocytes / Q000032:analysis; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D011247:Pregnancy; D011296:Prenatal Diagnosis; D013789:Thalassemia / Q000175:diagnosis* / Q000235:genetics
D015345:Oligonucleotide Probes; D004247:DNA