CD4-independent infection of human neural cells by human immunodeficiency virus type 1.
A number of studies have indicated that central nervous system-derived cells can be infected with human immunodeficiency virus type 1 (HIV-1). To determine whether CD4, the receptor for HIV-1 in lymphoid cells, was responsible for infection of neural cells, we characterized infectable human central nervous system tumor lines and primary fetal neural cells and did not detect either CD4 protein or mRNA. We then attempted to block infection with anti-CD4 antibodies known to block infection of lymphoid cells; we noted no effect on any of these cultured cells. The results indicate that CD4 is not the receptor for HIV-1 infection of the glioblastoma line U373-MG, medulloblastoma line MED 217, or primary human fetal neural cells.
Harouse
J M
JM
Department of Neurology, University of Pennsylvania Medical Center, Philadelphia 19104-6076.
Kunsch
C
C
Hartle
H T
HT
Laughlin
M A
MA
Hoxie
J A
JA
Wigdahl
B
B
Gonzalez-Scarano
F
F
D000914:Antibodies, Viral / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D001667:Binding, Competitive; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000382:microbiology*; D001932:Brain Neoplasms; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005434:Flow Cytometry; D005910:Glioma; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008527:Medulloblastoma; D009474:Neurons / Q000382:microbiology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D000914:Antibodies, Viral; D000945:Antigens, Differentiation, T-Lymphocyte