title: Endotoxic-lipopolysaccharide-specific binding proteins on lymphoid cells of various animal species: association with endotoxin susceptibility.
abstract: Endotoxic lipopolysaccharide (LPS), a common structural component of all gram-negative bacteria, is well recognized for its capacity to interact with and perturb immunologically relevant cells. Using a radioiodinated, photoactivatable LPS probe, we have recently identified an 80-kilodalton LPS-specific binding protein on murine B lymphocytes. We now have extended these studies to determine if other mammalian species, as well as representative endotoxin-resistant species (frog and chicken), have a similar LPS-binding protein. We have identified what appears to be a relatively conserved 80-kilodalton LPS-binding protein on mononuclear cells of all mammalian species tested. However, both frog and chicken leukocytes failed to show the presence of a similar LPS-binding protein. It is possible that the presence of specific LPS-binding proteins may be important for endotoxin sensitivity of most mammalian species.
authors: Department of Microbiology, University of Kansas Medical Center 66103.
authors: D J
authors: DJ
authors: Roeder
authors: M G
authors: MG
authors: Lei
authors: D C
authors: DC
authors: Morrison
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D004285:Dogs; D004731:Endotoxins / Q000378:metabolism* / Q000494:pharmacology; D006041:Goats; D006736:Horses; D008070:Lipopolysaccharides / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D011817:Rabbits; D011892:Rana catesbeiana; D012756:Sheep; D013045:Species Specificity; D013552:Swine
chemical_list: D002352:Carrier Proteins; D004731:Endotoxins; D008070:Lipopolysaccharides