MLR3 molecule is an activation antigen shared by human B, T lymphocytes and T cell precursors.
MLR3 molecule is a membrane glycoprotein (mol. mass range 28-34 kDa) present on activated, but not resting human peripheral T cells, B cells and thymocytes. Its kinetics of appearance on the cell surface (3 h after the addition of the inductive signal to the cells) suggests that it is an early activation antigen. The proliferative response of cultured T and B lymphocytes and thymocytes to different activation signals is inhibited by the addition of MLR3 monoclonal antibody. Moreover the antibody in combination with non-mitogenic doses of phorbol myristate acetate leads to proliferation of thymocytes and resting B and T lymphocytes. In the latter, synthesis of interleukin 2 is also induced. Biochemical analysis of MLR3 antigen indicates that it is a phosphorylated protein with N-linked sugar moieties. Together these data suggest a role for MLR3 antigen in the signal transduction process during activation, both for mature lymphocytes and for T cell precursors.
Risso
A
A
Instituto scientifico Tumori, Genova, Italy.
Cosulich
M E
ME
Rubartelli
A
A
Mazza
M R
MR
Bargellesi
A
A
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology* / Q000302:isolation & purification; D001402:B-Lymphocytes / Q000032:analysis / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D014066:Palatine Tonsil; D010766:Phosphorylation; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D015398:Signal Transduction; D013234:Stem Cells / Q000032:analysis / Q000276:immunology*; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D013950:Thymus Gland
D000954:Antigens, Surface; C059122:MLR3 activation antigen; D008562:Membrane Glycoproteins; D011948:Receptors, Antigen, T-Cell