Carbohydrate components recognized by the cone-specific monoclonal antibody CSA-1 and by peanut agglutinin are associated with red and green-sensitive cone photoreceptors.
Previous investigations have demonstrated that the monoclonal antibody CSA-1 and peanut agglutinin label specifically cone photoreceptor cells. In the present study, we compared the binding of CSA-1 and peanut agglutinin to that of the monoclonal antibodies COS-1 and OS-2, which have been shown to recognize the red/green- and blue-sensitive cone visual pigments, respectively. Using lectin and immunocytochemistry on serial semithin sections of the pig retina, we have demonstrated in the present study that both CSA-1 and peanut agglutinin label specifically the red-, and green-sensitive, but not the blue-sensitive cone cell outer segments. Peanut agglutinin does bind, however, to the cone matrix sheaths associated with all three types of cones. These observations support the idea that red-, and green-sensitive cone cells share some common molecular epitopes and may represent a differentiation line of cones, considerably different from that of blue-sensitive cones.
Röhlich
P
P
Department of Pathology, University of Texas Health Science Center, San Antonio 78284.
Szél
A
A
Johnson
L V
LV
Hageman
G S
GS
D000818:Animals; D000911:Antibodies, Monoclonal; D003118:Color Perception / Q000502:physiology*; D037102:Lectins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D019887:Peanut Agglutinin; D010786:Photoreceptor Cells / Q000378:metabolism*; D012168:Retinal Pigments / Q000276:immunology / Q000378:metabolism*; D013552:Swine
D000911:Antibodies, Monoclonal; D037102:Lectins; D008562:Membrane Glycoproteins; D019887:Peanut Agglutinin; D012168:Retinal Pigments