Molecule detected in formalin fixed tissue by antibodies MT1, DF-T1, and L60 (Leu-22) corresponds to CD43 antigen.
Three monoclonal antibodies MT1, L60 (Leu-22), and DF-T1, were reported independently as recognising human T cells in routinely processed, paraffin wax embedded tissue. The present study was performed to compare these three reagents in terms of their immunocytochemical reactions and target molecule(s). On Western blotting of white cell extracts the three antibodies reacted with antigens of the same molecular weight (range 110-160 kilodaltons). Furthermore, their immunocytochemical reactivity with normal human cells, as analysed by two-colour flow cytometry, was essentially identical (labelling of monocytes, most T lymphocytes, and weak reactions with some B cells), and the antibodies gave closely similar reactions on 54 white cell derived neoplasms. To identify the target antigen for these three reagents, antibodies from the Third International Workshop on Leucocyte Antigens were reviewed and it was shown that the Western blotting and immunocytochemical reactions of MT1, L60 (Leu-22), and DF-T1 were identical with those of the reagents which defined the CD43 antigen (also known as leucosialin or sialophorin). Furthermore, all these antibodies reacted with cells transfected with a cDNA clone encoding CD43. It is concluded that antibodies MT1, L60 (Leu-22), and DF-T1 all recognise the heavily glycosylated myeloid/lymphoid associated CD43 antigen.
Stross
W P
WP
Nuffield Department of Pathology, University of Oxford, John Radcliffe Hospital.
Warnke
R A
RA
Flavell
D J
DJ
Flavell
S U
SU
Simmons
D
D
Gatter
K C
KC
Mason
D Y
DY
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm / Q000032:analysis; D015153:Blotting, Western; D005404:Fixatives; D005434:Flow Cytometry; D005557:Formaldehyde; D006801:Humans; D007938:Leukemia / Q000276:immunology; D051917:Leukosialin; D008223:Lymphoma / Q000276:immunology; D012795:Sialoglycoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D005404:Fixatives; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins; D005557:Formaldehyde