title: [Formation of pAS8-1213 cointegrate with one of the plasmids of Azospirillum brasilense Sp245].
abstract: Inheritance of the plasmid vector pAS8-1213 in Azospirillum brasilense Sp245 cells has been studied. The plasmid pAS8-1213 is shown to be uncapable of autonomous replication in the new host but able to integrate into the genetic structures of Azospirillum with high frequency. 90-95% of KmR-transconjugants of A. brasilense harbor pAS8-1213 cointegrated with the smaller host plasmid pAbSP245c(85Md). The formed cointegrate can be transferred into Azospirillum spp. 75 and RecA- strains of E. coli (HB101 and DH1) and stably maintained in these cells. The IS21 element inherent of the plasmid pAS8-1213 is supposed to participate in pAS8-1213::pAbSP245c cointegrate formation.
authors: V Iu
authors: VIu
authors: Matveev
authors: L P
authors: LP
authors: Petrova
authors: E A
authors: EA
authors: Zhuravleva
authors: V I
authors: VI
authors: Panasenko
mesh_terms: D015806:Azospirillum brasilense / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D010957:Plasmids
vernacular_title: Obrazovanie kointegrata pAS8-1213 s odnoĭ iz plazmid Azospirillum brasilense Sp245.