Biochemical characterization of the DNA polymerase activity present in isolated nuclei from mussel tissues: a possible system for the evaluation of the rate of DNA repair.
1. The nuclear DNA polymerase activity in mussel digestive glands was characterized regarding Mg++ requirement (2 mM), ATP concentration (4 mM), pH (8.4), and ionic strength (50 mM). 2. Most of the enzymatic activity is aphidicolin insensitive, probably due to DNA polymerase beta-like activity. 3. The exposure of mussels to a genotoxic organic compound such as dimethylsulfate (DMS) resulted in increases of about 250% and 100% in the DNA polymerase activity in nuclei isolated from the gills and digestive glands, respectively. 4. These data indicate that the isolated nuclei from mussel tissues may be utilized for the evaluation of the rate of DNA repair synthesis that reflects the DNA damage induced in vivo by exposure of the animals to genotoxic compounds.
Accomando
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Istituto di Fisiologia Generale, Universitá di Genova, Italy.
Viarengo
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Zonchedu
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Orunesu
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D000818:Animals; D049872:Bivalvia / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology*; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009153:Mutagens / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D013463:Sulfuric Acid Esters / Q000494:pharmacology
D009153:Mutagens; D013463:Sulfuric Acid Esters; D011189:Potassium Chloride; D004247:DNA; D004259:DNA-Directed DNA Polymerase; C007482:dimethyl sulfate