Anti-idiotypic antibodies to a monoclonal antibody raised against anti-müllerian hormone exhibit anti-müllerian biological activity.
Polyclonal anti-idiotypic antibodies were raised to three monoclonal antibodies to bovine anti-Müllerian hormone, and purified by affinity chromatography. All anti-idiotypes inhibited binding of labelled anti-Müllerian hormone to the monoclonal antibody against which they were directed; in addition, the anti-idiotypes directed against a non-zoospecific monoclonal antibody inhibited binding of labelled anti-Müllerian hormone to a monoclonal antibody raised against human testicular AMH, indicating that the idiotype against which these anti-idiotypes are directed recognizes a conserved epitope on the anti-Müllerian hormone molecule. These anti-idiotypes, but not those directed against other monoclonals, exhibit hormone-like activity in a bioassay for anti-Müllerian activity, and we suggest that they may act as antibodies against the anti-Müllerian hormone receptor.
Lefèvre
G
G
Unité de Recherches sur l'Endocrinologie du Développment, INSERM, Hôpital des Enfants-Malades, Paris, France.
Tran
D
D
Hoebeke
J
J
Josso
N
N
D000818:Animals; D054304:Anti-Mullerian Hormone; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D006023:Glycoproteins; D006131:Growth Inhibitors / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009095:Mullerian Ducts; D051381:Rats; D013735:Testicular Hormones / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D013737:Testis / Q000032:analysis / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D006131:Growth Inhibitors; D007130:Immunoglobulin Idiotypes; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone