The effect of lipopolysaccharides (LPS) on generation of thromboxane A2 and thromboplastin activity in whole blood of males, females and females on oral contraceptives.
Heparinized blood sample taken from 81 healthy persons, 40 men and 41 women of whom 17 were on combined oral contraceptives, were incubated with 2 ng lipopolysaccharides (LPS)/ml blood. Quantitation of thromboxane B2 (TxB2) in the resultant plasma revealed a significantly (p less than 0.001) higher generation of TxB2 in men than in women. No sexual differences were observed in thromboplastin activity in unstimulated and LPS stimulated monocytes. Addition of liposomes together with LPS enhanced the synthesis of thromboplastin 2-10 fold and unveiled a higher synthesis of thromboplastin among men. No differences were observed in TxB2 and thromboplastin synthesis in blood collected from females or females using oral contraceptives. A significant correlation between TxB2 and the thromboplastin activity exists within the male (r = 0.46, p = 0.003) and the oral contraceptive (r = 0.57, p = 0.016) group after exposure to LPS. This study suggests that males exposed to LPS may generate more thromboxane A2 than females and thus be at higher risk of developing thrombosis.
Hansen
J B
JB
Institute of Clinical Medicine, University of Tromsø, Norway.
Wilsgård
L
L
Olsen
J O
JO
Svensson
B
B
Osterud
B
B
D000328:Adult; D003278:Contraceptives, Oral, Hormonal / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008081:Liposomes; D008262:Macrophage Activation; D008297:Male; D009000:Monocytes / Q000378:metabolism; D012307:Risk Factors; D012737:Sex Factors; D013925:Thromboplastin / Q000096:biosynthesis / Q000378:metabolism*; D013928:Thromboxane A2 / Q000097:blood*; D013929:Thromboxane B2 / Q000096:biosynthesis / Q000097:blood
D003278:Contraceptives, Oral, Hormonal; D008055:Lipids; D008070:Lipopolysaccharides; D008081:Liposomes; D013929:Thromboxane B2; D013928:Thromboxane A2; D013925:Thromboplastin