title: CD8+CD4- lymphocyte lines can harbor the AIDS virus in vitro.
abstract: A detailed definition of AIDS virus-specific T lymphocytes will require the generation and characterization of HIV-1-specific, cloned T cell populations. In our studies, we show that CD8+CD4- lymphocyte lines, derived from PBL of rhesus monkeys infected with simian immunodeficiency virus of macaques and humans infected with HIV-1, can harbor AIDS viruses. CD8+CD4- lymphocyte lines derived from infected individuals are shown to express AIDS virus-encoded proteins and generate reverse transcriptase activity. Infection of these CD8+CD4- lymphocytes is confirmed at the single cell level by the techniques of immunoelectronmicroscopy and two-color immunohistochemistry. This observation suggests that it may prove problematic to generate cloned, functional T lymphocyte populations from AIDS virus-infected individuals and raises the possibility that CD8+CD4- cells may serve as reservoirs for the AIDS viruses.
authors: Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.
authors: H
authors: H
authors: Tsubota
authors: D J
authors: DJ
authors: Ringler
authors: M
authors: M
authors: Kannagi
authors: N W
authors: NW
authors: King
authors: K R
authors: KR
authors: Solomon
authors: J J
authors: JJ
authors: MacKey
authors: D G
authors: DG
authors: Walsh
authors: N L
authors: NL
authors: Letvin
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D015497:HIV-1 / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D008253:Macaca mulatta; D010641:Phenotype; D012191:Retroviridae Proteins / Q000096:biosynthesis; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification* / Q000382:microbiology / Q000648:ultrastructure; D014779:Virus Replication
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D012191:Retroviridae Proteins