Interleukin-1 stimulates human chorionic gonadotropin secretion by first trimester human trophoblast.
Interleukin-1 (IL-1) has been reported to stimulate LH, GH, ACTH, and TSH release from cultured pituitary cells. IL-1 also has been found to be secreted in significant amounts by placental macrophages. To determine the possible role of IL-1 within the placenta, we studied the effects of human recombinant IL-1 on hCG release by long term cultures of human first trimester trophoblast and the JAR (human choriocarcinoma) cell line. IL-1 in concentrations ranging from 10(-11)-10(-9) mol/L stimulated hCG release from trophoblast cultures. This stimulatory effect was not mediated by prostaglandin E2 (PGE2), as indicated by the inability of indomethacin to block this stimulation as well as a lack of IL-1 effect on PGE2 release by trophoblast cells. At these doses, IL-1 exerted no effect on hCG release by JAR cells. PGE2, when used in high concentrations (10(-6)-10(-5) mol/L), stimulated the release of hCG by the trophoblasts as well as by the JAR cells. Neither IL-1 nor PGE2 stimulated the proliferation, [3H]thymidine incorporation, or differentiation (syncytium formation) of trophoblast or JAR cells. These results suggest that IL-1 may be an important local regulator of hCG secretion by first trimester trophoblasts.
Yagel
S
S
Department of Anatomy, University of Western Ontario, London, Toronto, Canada.
Lala
P K
PK
Powell
W A
WA
Casper
R F
RF
D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D002822:Choriocarcinoma; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D015232:Dinoprostone / Q000494:pharmacology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011994:Recombinant Proteins / Q000494:pharmacology; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D007375:Interleukin-1; D011994:Recombinant Proteins; D015232:Dinoprostone