title: Secretory hydrolases of Trichomonas vaginalis.
abstract: Supernatants of three Trichomonas vaginalis strains obtained from culture or after contact and cultivation with WISH cells were assayed to characterize the nature of the factor/s responsible for the cytotoxic activity on WISH cell monolayers. This factor, mainly secreted from the live, exponentially grown parasite, with a molecular weight higher than 10,000 daltons and heat-unstable, does not appear to be a protease. In its place, we demonstrated that the presence of some hydrolases (particularly acid phosphatase and beta-N-acetylglucosaminidase) was related to the cytotoxic activity of T. vaginalis strains. Hence, some hydrolytic enzymes may indicate the pathogenetic role of a T. vaginalis strain.
authors: Istituto di Microbiologia, Università di Torino, Italy.
authors: D
authors: D
authors: Savoia
authors: M G
authors: MG
authors: Martinotti
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003603:Cytotoxins / Q000032:analysis*; D006801:Humans; D006867:Hydrolases / Q000032:analysis*; D014246:Trichomonas vaginalis / Q000201:enzymology*
chemical_list: D003603:Cytotoxins; D006867:Hydrolases