Establishment and characterization of a new human cell-line (AMOC-2) derived from a serous adenocarcinoma of ovary.
A new cell line, designated as AMOC-2, was established from a mouse graft of human ovarian serous adenocarcinoma, and has grown well without interruption for over 12 months. The cultured cells of the AMOC-2 line were spindle or polygonal in shape and showed a monolayer growth with a pavement-like arrangement and a slight tendency to pile up, thus lacking contact inhibition. This cell line has a doubling time of 17.4h, a saturation density of 9.17 X 10(4) cells/cm2, a plating efficiency of 36.2% and a mitotic coefficient of 6.2%. The chromosomal number of this cell line is distributed in the diploid range and three of the specific marker chromosomes were identified in the cells with 49 chromosomes. This cell line was able to be transplanted into the subcutis of nude mice and produced a poorly differentiated serous adenocarcinoma resembling the original tumor. These cells have maintained their tumorigenic capacity despite prolonged maintenance in culture and are now in the 60th passage.
Yabushita
H
H
Department of Obstetrics and Gynecology, Aichi Medical University.
Ueno
N
N
Sawaguchi
K
K
Higuchi
K
K
Noguchi
M
M
Ishihara
M
M
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D003536:Cystadenocarcinoma / Q000235:genetics / Q000473:pathology*; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured