title: Solubilizing the T-cell receptor--problems in solution.
abstract: Recombinant DNA technology has been central in answering some of the most important questions in immunology and has recently helped to define the complex of membrane associated proteins on T-cell surfaces responsible for antigen recognition. Here André Traunecker and colleagues describe attempts to facilitate the analysis of this complex using genetic engineering to produce solubilized T-cell receptor and associated molecules.
authors: A
authors: A
authors: Traunecker
authors: B
authors: B
authors: Dolder
authors: F
authors: F
authors: Oliveri
authors: K
authors: K
authors: Karjalainen
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000302:isolation & purification; D003001:Cloning, Molecular / Q000379:methods*; D005813:Genes, Synthetic; D000949:Histocompatibility Antigens Class II / Q000302:isolation & purification; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification*; D051379:Mice; D011487:Protein Conformation; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012995:Solubility; D013601:T-Lymphocytes / Q000032:analysis
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins