title: Effect of sodium arsenite on the induction and turnover of ornithine decarboxylase activity in erythroleukemia cells.
abstract: Sodium arsenite proved effective in preventing the induction of ornithine decarboxylase (ODC) activity elicited by dilution of Friend erythroleukemia cells in fresh medium. A 50 per cent inhibition was produced at approximately 1 microM arsenite and complete inhibition was obtained at concentrations above 10 microM. However, addition of arsenite 5 h after cell dilution, i.e. when ODC was already induced, appeared to stabilize the enzyme. The half-life of ODC activity, measured after cycloheximide treatment, increased almost six-fold after addition of sodium arsenite. Agents known to provoke oxidative alteration of the thiol-redox status in cells, also caused a similar effect on the induction and stability of ODC.
authors: Dipartimento di Biochimica, Università di Bologna, Italy.
authors: F
authors: F
authors: Flamigni
authors: S
authors: S
authors: Marmiroli
authors: C M
authors: CM
authors: Caldarera
authors: C
authors: C
authors: Guarnieri
mesh_terms: D000818:Animals; D001151:Arsenic / Q000494:pharmacology*; D018053:Arsenites; D002330:Carmustine / Q000494:pharmacology; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction; D005622:Friend murine leukemia virus; D006207:Half-Life; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology* / Q000378:metabolism; D051379:Mice; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000378:metabolism; D010084:Oxidation-Reduction; D010545:Peroxides / Q000494:pharmacology; D017670:Sodium Compounds; D020122:tert-Butylhydroperoxide
chemical_list: D018053:Arsenites; D010545:Peroxides; D017670:Sodium Compounds; C017947:sodium arsenite; D020122:tert-Butylhydroperoxide; D003513:Cycloheximide; D009955:Ornithine Decarboxylase; D001151:Arsenic; D002330:Carmustine