Components of the human T lymphocyte antigen receptor complex, CD3-p28 and CD3-gamma, are biochemically distinct.
A recently described subunit of the T cell antigen receptor-CD3 complex, p28, is very similar to the mature CD3-gamma subunit with respect to electrophoretic mobility. We demonstrated, by one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and endo-beta-n-acetylglucosaminidase digestion, that CD3-p28 and CD3-gamma coexist in CD3-specific immunoprecipitates from the human T lymphocyte hybrid cell line 255.88, which was labeled by biosynthetic incorporation of radioactive amino acids. By partial proteolysis and peptide mapping, we demonstrated that CD3-p28 and CD3-gamma are distinct members of the CD3 complex.
Stewart
S J
SJ
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN.
Levy
R
R
D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007120:Immunochemistry; D010449:Peptide Mapping; D011233:Precipitin Tests; D011948:Receptors, Antigen, T-Cell
D011948:Receptors, Antigen, T-Cell