title: Induction of cytochrome P-450c and P-450d by metyrapone in the primary culture of rat hepatocytes.
abstract: The effects of metyrapone on qualitative changes in cytochrome P-450-dependent drug metabolizing activities in primary cultures of rat hepatocytes were investigated. Metyrapone apparently increased benzo(a)pyrene hydroxylation and maintained both ethoxycoumarin-O-deethylation and propoxycoumarin-O-depropylation, whereas it had little effect on methoxycoumarin-O-demethylation. Furthermore, P-450d (high spin type of P-448) as well as P-450c (low spin type of P-448) were induced by metyrapone, while P-450b and P-450e were not. In conclusion, metyrapone act as a 3-methylcholanthrene-like inducer in the primary cultures of rat hepatocytes.
authors: Department of Pharmacology, National Institute of Hygienic Sciences, Tokyo, Japan.
authors: K
authors: K
authors: Hirota
authors: T
authors: T
authors: Kawanishi
authors: M
authors: M
authors: Sunouchi
authors: Y
authors: Y
authors: Ohno
authors: A
authors: A
authors: Takanaka
authors: Y
authors: Y
authors: Yamazoe
authors: R
authors: R
authors: Kato
authors: Y
authors: Y
authors: Murakoshi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008797:Metyrapone / Q000494:pharmacology*; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010634:Phenobarbital; D008797:Metyrapone