title: High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes.
abstract: Mammalian metaphase chromosomes can be identified by their characteristic banding pattern when stained with Giemsa dye after brief proteolytic digestion. The resulting G-bands are known to contain regions of DNA enriched in A/T residues and to be the principal location for the L1 (or Kpn 1) family of long interspersed repetitive sequences in human chromosomes. Here we report that antibodies raised against a highly purified and biochemically well characterized nonhistone "High-Mobility Group" protein, HMG-I, specifically localize this protein to the G-bands in mammalian metaphase chromosomes. In some preparations in which chromosomes are highly condensed, HMG-I appears to be located at the centromere and/or telomere regions of mammalian chromosomes as well. To our knowledge, this is the first well-characterized mammalian protein that localizes primarily to G-band regions of chromosomes.
authors: Program in Genetics and Cell Biology, Washington State University, Pullman 99164-4660.
authors: J E
authors: JE
authors: Disney
authors: K R
authors: KR
authors: Johnson
authors: N S
authors: NS
authors: Magnuson
authors: S R
authors: SR
authors: Sylvester
authors: R
authors: R
authors: Reeves
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006609:High Mobility Group Proteins / Q000032:analysis*; D006801:Humans; D007399:Interphase; D004915:Leukemia, Erythroblastic, Acute; D008677:Metaphase; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured / Q000166:cytology
chemical_list: D006609:High Mobility Group Proteins