Proliferation of interleukin 2 dependent cytotoxic T cell line cells. Different protein kinase C activators achieve the same maximal promotion, but with distinct dose-response profiles.
Protein kinase C activators of the teleocidin family are claimed to be able to replace interleukin 2 for inducing short-term proliferation of the interleukin 2 dependent murine cytotoxic T cell line. While teleocidin B4 was found to be much more active than 12-O-tetradecanoyl-phorbol-13-acetate, a related molecule showing a substitution of a single OH by OCH3, olivoretin A, lacked detectable activity in this assay. However, the maximal proliferation achievable by protein kinase C activators did not exceed one third of the one given by recombinant interleukin 2.
Gavériaux
C
C
Preclinical Research Department, Sandoz Ltd., Basel, Switzerland.
Loor
F
F
D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2; D008213:Lymphocyte Activation / Q000187:drug effects; D008235:Lyngbya Toxins / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007376:Interleukin-2; D008235:Lyngbya Toxins; C022176:teleocidins; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate