Isolation and structure of ranamargarin, a new tachykinin from the skin of Chinese frog Rana margaratae.
A new tetradecapeptide, ranamargarin, has been isolated by Sep-Pak C18 and HPLC from methanol extracts of the skin of the Chinese frog Rana margaratae. The sequence of the peptide is: Asp-Asp-Ala-Ser-Asp-Arg-Ala-Lys-Lys-Phe-Tyr-Gly-Leu-Met-NH2. This structure has been confirmed by synthesis. The peptide is the largest among the amphibian tachykinins and its N-terminal amino acids are quite different from those of the other tachykinins. The formation of the sulfoxide and peak-splitting of ranamargarin during purification procedures are briefly discussed.
Tang
Y Q
YQ
Shanghai Institute of Materia Medica, Academia Sinica.
Tian
S H
SH
Wu
S X
SX
Hua
J C
JC
Wu
G F
GF
Zhao
E M
EM
Lu
Y A
YA
Zhu
Y Q
YQ
Zou
G
G
Tsou
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D011898:Ranidae; D012867:Skin / Q000032:analysis*; D015320:Tachykinins / Q000302:isolation & purification*
D015320:Tachykinins; C061715:ranamargarin