Comparison of two methods for evaluation of the length of collagen molecules.
Two microscopical methods for the evaluation of molecular length--the measurement of SLS aggregates and the rotary shadowing technique--are compared. Two types of collagen were used for the comparison: type I collagen, isolated by acid extraction from calf skin, and type IX, isolated from chicken cartilage. An average length of 303 nm was obtained for type I collagen (by measurement of SLS aggregates 247 nm). Type IX collagen forms three different types of SLS crystallites corresponding to molecular lengths of 61, 95 and 206 nm; with the rotary shadowing technique, lengths of 84, 116 and 197 nm were measured.
Miterová
L
L
Stokrová
J
J
Hulejová
H
H
Adam
H
H
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000648:ultrastructure*; D008722:Methods
D003094:Collagen