Combined treatment with vitamins E and C in experimental myocardial infarction in pigs.
The effect of two different combined treatments with vitamin E acetate and vitamin C on infarct size and recovery of regional myocardial function was investigated in ischemic, reperfused porcine hearts. The left anterior descending coronary artery was distally ligated in 30 thoracotomized pigs for 45 minutes followed by 3 days of reperfusion. Infarct size was determined as the ratio of infarcted (tetrazolium stain) to ischemic (dye technique) myocardium. Regional myocardial function was assessed by sonomicrometry. Ten pigs received vitamin E acetate (12 gm intravenously three times for 1 week) before ischemic and vitamin C (4.4 gm intravenously) before reperfusion (therapy A). Another 10 pigs were treated with vitamin E acetate (12 gm intraarterially) and vitamin C (4.4 gm intravenously) during ischemia (therapy B). An additional 10 pigs served as a control group. Global hemodynamics did not differ significantly among the groups before and during ischemia. Mean plasma concentrations of vitamin E amounted to 107 micrograms/ml in group A, 16 micrograms/ml in group B, and 0.9 micrograms/ml in the control group at the onset of reperfusion. Therapy A reduced the size of the infarct from 73 +/- 12% to 47 +/- 16% of the region at risk (p less than 0.005) and improved regional systolic shortening from 0 +/- 7% to 11 +/- 6% at 3 days after reperfusion (p less than 0.01). Therapy B decreased the size of the infarct to 64 +/- 9% of the region at risk (p = 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Klein
H H
HH
Department of Cardiology, University of Göttingen, West Germany.
Pich
S
S
Lindert
S
S
Nebendahl
K
K
Niedmann
P
P
Kreuzer
H
H
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000032:analysis; D004359:Drug Therapy, Combination; D005609:Free Radicals; D006439:Hemodynamics / Q000187:drug effects; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000503:physiopathology / Q000517:prevention & control; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000032:analysis; D013552:Swine; D014810:Vitamin E / Q000008:administration & dosage* / Q000032:analysis
D005609:Free Radicals; D014810:Vitamin E; D001205:Ascorbic Acid