Exposure level monitor of a carcinogenic glutamic acid pyrolysis product in rabbits.
In order to determine a suitable indicator for estimating the exposure levels of the dietary carcinogen 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1), a carcinogenic glutamic acid pyrolysis product, the levels of Glu-P-1 bound to blood components were monitored for 8 weeks by a high-performance liquid chromatography method after the dietary carcinogen was administered as single oral doses (0.2-50 mg) to rabbits. In all rabbits dosed with Glu-P-1, Glu-P-1 in erythrocytes was detectable even on day 42 after administration. Glu-P-1 in plasma disappeared faster than did Glu-P-1 in erythrocytes. Glu-P-1 levels in rabbit hemoglobin were linearly related to administered doses at all points of time investigated. The results suggest that Glu-P-1 covalently bound to hemoglobin is very suitable for monitoring long-term exposure levels.
Yin
Y
Y
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Wada
O
O
Manabe
S
S
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D004912:Erythrocytes / Q000032:analysis; D007093:Imidazoles / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D013058:Mass Spectrometry; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D007093:Imidazoles; C023785:2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole