[Transcription map of a recombinant plasmid carrying a gene for the synthesis of pesticine I and a protein for pesticine I immunity in the plague microbe].
Molecules of the plasmids pBR322 and pRD17 have been compared by electron microscopy technique of R-loops visualization. Comparative location of R-loops on the plasmids has been computerized on minicomputer HP9825A due to the program making possible to define the coordinates of the transcription start and direction. The 5.1 kb fragment coding for pesticin I and immunity protein to pesticin I and cloned in pBR322 vector plasmid is flanked by Bam HI-EcoRI sites. Five promoter regions and direction of transcription were localized on the fragment.
Sorokin
V M
VM
Goncharov
E K
EK
Mishan'kin
B N
BN
D001430:Bacteriocins / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000648:ultrastructure; D005798:Genes, Bacterial; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic; D015010:Yersinia pestis / Q000235:genetics* / Q000276:immunology
D001430:Bacteriocins; D004269:DNA, Bacterial; C011330:Pesticin
Transkriptsionnaia karta rekombinantnoĭ plazmidy, nesushcheĭ geny sinteza pestitsina I i belka immunnosti k pestitsinu I chumnogo mikroba.