[Effect of diglycylhistidine on the oxidation of ascorbate catalyzed by Cu(II)].
Glycylglycylhistidine (GGH) was synthesized using mixed anhydride method, and Cu(II): GGH = 1:1 complex was identified using UV, ESR, CD, ORD and FAB/MS technique. The oxidation of ascorbate catalyzed by cupric ion in RPMI 1640 medium (pH 7.40) was studied. The activation energy E = 39.2 kJ/mol. The addition of GGH inhibited the oxidation of ascorbate catalyzed by cupric ion. The investigations with ESR and fluorimetry indicate that there are Cu(I), ascorbate radical (AH), O2-., H2O2 and .OH during the oxidation.
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D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001205:Ascorbic Acid / Q000378:metabolism*; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology; D003300:Copper; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects
D009842:Oligopeptides; C037026:diglycyl-histidine; D003300:Copper; D001205:Ascorbic Acid