title: Effects of dietary n - 3 fatty acids on mass changes and [3H]glycerol incorporation in various glycerolipid classes of rat kidney in vivo.
abstract: The effects of dietary fish oil containing n - 3 polyunsaturated fatty acids on triacylglycerol synthesis and phospholipid metabolism (including the alkylacyl subclass of choline glycerophospholipids (CGP)) was studied in rat kidney in vivo. After a 3 week feeding period, the triacylglycerol content (in mumol/g kidney) was 47% lower in the fish oil group relative to animals given sunflower oil. This alteration was accompanied by a substantially lower amount of arachidonic acid (20:4(n - 6)) and higher level (mumol/g tissue) of eicosapentaenoic acid (20:5(n - 3)) plus docosahexaenoic acid (22:6(n - 3)) in the triacylglycerol, CGP, and ethanolamine glycerophospholipids (EGP) of the fish oil group. The labelling of triacylglycerol relative to phospholipid from [3H]glycerol following i.p. administration was 49% lower in the fish oil as compared to the sunflower oil group, indicating a suppression of renal triacylglycerol synthesis relative to phospholipid synthesis. Modest differences in the labelling of CGP and EGP were found. A moderate and significantly lower proportional labelling (by 35%) of the alkylacyl subclass of CGP was observed in the fish oil as compared to the sunflower oil animals. These findings may have relevance to eicosanoid and platelet activating factor (PAF) biosyntheses as well as renal function and pathophysiology.
authors: Department of Nutritional Sciences, University of Guelph, Canada.
authors: Y K
authors: YK
authors: Yeo
authors: D J
authors: DJ
authors: Philbrick
authors: B J
authors: BJ
authors: Holub
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D010743:Phospholipids / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology; D051381:Rats; D000074242:Sunflower Oil; D014280:Triglycerides / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
chemical_list: D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D010743:Phospholipids; D010938:Plant Oils; D000074242:Sunflower Oil; D014280:Triglycerides; D005990:Glycerol