An improved enzyme-linked immunosorbent assay of anti-collagen antibodies in human serum.
An improved enzyme-linked immunosorbent assay (ELISA) for the determination of anti-collagen antibodies in human serum has been developed. The method is based on the use of serum samples diluted to 1/50 with heat-inactivated normal rabbit serum adjusted to pH 8.0 with solid Tris (0.05 M), NaCl (0.15 M) and 2 M HCl. The use of normal rabbit serum minimizes non-specific adsorption of immunoglobulin G onto the plastic surface of microtiter plate. The applicability of the method for the quantitation of anti-collagen antibodies in human serum is demonstrated with 290 specimens of sera from normal controls (194) and patients with rheumatoid arthritis (96).
Fujii
K
K
Department of Orthopaedic Surgery, Jikei University School of Medicine, Tokyo, Japan.
Tsuji
M
M
Murota
K
K
Terato
K
K
Shimozuru
Y
Y
Nagai
Y
Y
D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D003094:Collagen / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis; D013048:Specimen Handling
D000906:Antibodies; D007074:Immunoglobulin G; D003094:Collagen