Fluorimetric determination of aluminium in serum.
A convenient fluorimetric method for the routine determination of aluminium in serum has been developed using lumogallion [4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulphonic acid]. Losses of aluminium during deproteinisation of the serum were prevented by treatment with a combination of 20 or 30% m/V trichloroacetic acid (TCA) and 5% m/VTCA. Iron(III) was removed by extraction into chloroform with capriquat (methyltrioctylammonium chloride) as an Fe3+-lumogallion-capriquat ternary complex. The interference from Cu2+ was eliminated by using thiosulphate as a masking agent. The detection limit was 3.6 ng ml-1 and the calibration graph was linear up to 1.4 micrograms ml-1 of aluminium. Using the proposed method, the average concentration of aluminium in the serum of healthy subjects was found to be 6.8 ng ml-1, in agreement with values reported in the literature.
Suzuki
Y
Y
Imai
S
S
Kamiki
T
T
D000535:Aluminum / Q000097:blood*; D001557:Benzenesulfonates; D003300:Copper / Q000097:blood; D005290:Ferric Compounds / Q000097:blood; D006801:Humans; D000644:Quaternary Ammonium Compounds; D012016:Reference Values; D013050:Spectrometry, Fluorescence
D001557:Benzenesulfonates; D005290:Ferric Compounds; D000644:Quaternary Ammonium Compounds; C061331:4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid; C037759:trioctylmethylammonium; D003300:Copper; D000535:Aluminum