title: Fatty acyl-coenzyme A is required for budding of transport vesicles from Golgi cisternae.
abstract: We describe a new role for fatty acylation. Conditions were established under which vesicular transport from the cis to the medial Golgi compartment in vitro depends strongly upon the addition of a fatty acyl-coenzyme A, e.g., palmitoyl-CoA. Using an inhibitor of long-chain acyl-CoA synthetase, we demonstrate that the fatty acid has to be activated by CoA to stimulate transport. A nonhydrolyzable analog of palmitoyl-CoA competitively inhibits transport. Electron microscopy and biochemical studies show that fatty acyl-CoA is required for budding of (non-clathrin-) coated transport vesicles from Golgi cisternae and that budding is inhibited by the nonhydrolyzable analog.
authors: Department of Biology, Princeton University, New Jersey 08544-1014.
authors: N
authors: N
authors: Pfanner
authors: L
authors: L
authors: Orci
authors: B S
authors: BS
authors: Glick
authors: M
authors: M
authors: Amherdt
authors: S R
authors: SR
authors: Arden
authors: V
authors: V
authors: Malhotra
authors: J E
authors: JE
authors: Rothman
mesh_terms: D000214:Acyl Coenzyme A / Q000502:physiology*; D000438:Alcohols / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002474:Cell-Free System; D003065:Coenzyme A / Q000502:physiology; D006224:Cricetinae; D003902:Detergents / Q000494:pharmacology; D006056:Golgi Apparatus / Q000502:physiology*; D006868:Hydrolysis; D007425:Intracellular Membranes / Q000502:physiology; D017830:Octoxynol; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology
chemical_list: D000214:Acyl Coenzyme A; D000438:Alcohols; D003902:Detergents; D010171:Palmitoyl Coenzyme A; D011092:Polyethylene Glycols; D017830:Octoxynol; D003065:Coenzyme A