Characterization of a mouse mitogen-regulated protein/proliferin gene and its promoter: a member of the growth hormone/prolactin gene superfamily.
A genomic clone containing the complete gene, designated mrp/plf3, of a member of the mitogen-regulated protein/proliferin (MRP/PLF) multi-gene family was isolated from a mouse genomic library constructed in lambda L47.1. The DNA sequences of the 5' flanking region, the exons, and the exon/intron boundaries have been determined. The size and organization of the five exons in the murine mrp/plf3 gene are very similar to those of members of the prolactin/growth hormone family, confirming that mrp/plf3 is a part of this superfamily. Removal of an upstream negative regulatory element revealed an active mrp/plf promoter that was responsive to TGF-alpha and contains known regulatory elements. Intracellular mrp/plf mRNA levels were increased by 17 beta-estradiol and reduced by dexamethasone.
Connor
A M
AM
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
Waterhouse
P
P
Khokha
R
R
Denhardt
D T
DT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D005091:Exons; D006023:Glycoproteins / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D011388:Prolactin / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C000634106:Prl2c2 protein, mouse; D012333:RNA, Messenger; D011388:Prolactin; D013006:Growth Hormone; D004247:DNA