Immature and advanced patterns of T cell receptor gene rearrangement among lymphocytes in splenic culture.
Bulk populations and 39 hybridomas from splenic Con A cultures were analyzed for rearrangements among TCR genes: alpha, beta, gamma, and delta. Patterns were categorized to reveal general rules governing gene rearrangement within the activated adult peripheral population. Many patterns of gene rearrangement were consistent with previous studies of T cell lines. Additional points of interest were the following: 1) A large proportion of Con A-stimulated splenic cells bore no TCR gene rearrangements. 2) One splenic hybridoma exhibited an unusual gene pattern, with rearrangements, at alpha and beta, but not J gamma 1 or J gamma 2 loci. 3) Multiple gamma rearrangements were noted other than V1.2-J2 and V2-J1. 4) One hybridoma exhibited TCR gene rearrangements typical of day 14 to 15 fetal thymocytes, as well as rearrangements at immunoglobulin gene loci. 5) Among hybridomas with J alpha rearrangements, homologous chromosomes exhibited rearrangements at similar positions along the J alpha locus.
Hurwitz
J L
JL
Basel Institute for Immunology, Switzerland.
Samaridis
J
J
Pelkonen
J
J
D000375:Aging; D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A; D015342:DNA Probes; D015329:Gene Rearrangement, T-Lymphocyte; D005803:Genes, Immunoglobulin; D006825:Hybridomas / Q000032:analysis / Q000378:metabolism; D007133:Immunoglobulin Joining Region / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology / Q000502:physiology*
D015342:DNA Probes; D007133:Immunoglobulin Joining Region; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A