Cloning and analysis of the gene encoding the 230-kilodalton merozoite surface antigen of Plasmodium yoelii.
The complete nucleotide sequence of the gene for the 230-kDa precursor to the major merozoite surface antigens (PMMSA) of Plasmodium yoelii YM has been determined. A single open reading frame of 5316 bp encodes a protein of calculated molecular mass 197,000. The deduced amino acid sequence contains potential signal peptide and membrane anchor sequences of 19 and 18 residues, respectively, and a region of six tandemly repeated tetrapeptides, Gly-Ala-Val-Pro. Comparison with the 195-kDa Plasmodium falciparum analogue (Pf195) at the amino acid level indicated an overall homology of approximately 30%, with areas of as high as 60% conservation. The tripeptide repeats present near the N-terminus of the Pf195 antigen are absent from the Py230 sequence. The PMMSA can be divided into 22 blocks based upon interspecies amino acid conservation.
Lewis
A P
AP
Department of Molecular Biology, Wellcome Biotech, Beckenham, Kent, U.K.
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010967:Plasmodium yoelii / Q000235:genetics* / Q000276:immunology; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000953:Antigens, Protozoan; D000954:Antigens, Surface