Effect of vaccination on cell populations in lung washes from calves after infection with respiratory syncytial virus.
The inflammatory response in the air-passages of the lungs of calves after intranasal inoculation with respiratory syncytial virus (RSV) was compared in RSV-vaccinated and control animals. Total cells recovered from lung washings remained the same; however, the fold by eight days after infection and the type of cells changed from a predominance (85 per cent) of macrophages to equal proportions of macrophages and neutrophils (45 per cent) during the course of infection. The absolute numbers of neutrophils rose by 15-fold. In contrast, when RSV-vaccinated calves were challenged, the total number of cells recovered from lung washings remained the same; however, the numbers of macrophages decreased and the numbers of neutrophils increased by fivefold. Cytological studies of the lung washings revealed no evidence of an exacerbated inflammatory response in RSV-vaccinated calves. Levels of virus replication were significantly reduced in RSV-vaccinated compared with control animals.
Taylor
G
G
AFRC Institute for Animal Health, Compton Laboratory, Berkshire.
Thomas
L H
LH
Stott
E J
EJ
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000473:pathology; D002452:Cell Count; D004797:Enzyme-Linked Immunosorbent Assay; D008168:Lung / Q000473:pathology*; D008264:Macrophages; D009305:Nasopharynx / Q000382:microbiology; D009504:Neutrophils; D012136:Respiratory Syncytial Viruses / Q000276:immunology* / Q000502:physiology; D010253:Respirovirus Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D014779:Virus Replication
D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines