Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes.
A number of studies have suggested that resistance of target cells to natural killing (NK) may be correlated with their level of expression of major histocompatibility complex (MHC) class I antigens. To examine this hypothesis directly, a NK-sensitive class I-deficient human B-cell line was transfected with MHC class I genes. The expression of transfected HLA, but not H-2, class I gene products resulted in loss of susceptibility to human NK-mediated conjugation and cytolysis. Furthermore, this protection did not extend to cytotoxicity mediated by interleukin 2-stimulated human NK effector cells.
Storkus
W J
WJ
Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.
Alexander
J
J
Payne
J A
JA
Dawson
J R
JR
Cresswell
P
P
D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005805:Genes, MHC Class I; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D009154:Mutation; D012016:Reference Values; D014162:Transfection