T-cell colony formation in homosexual men with anti-human immunodeficiency virus.
In vitro differentiation and proliferation of precursor T-cells was examined in four homosexual men positive for anti-human immunodeficiency virus (HIV), one of whom had acquired immunodeficiency syndrome (AIDS). Peripheral mononuclear cells from the men were cultured with phytohemagglutinin-P in semi-solid culture media containing methylcellulose for 7 days. Colony formation was significantly reduced in all subjects, even in the presence of IL-2. Moreover, numerous colony component cells lacked T-cell specific surface markers. These results suggest that the impaired T-cell differentiation and proliferation occurs at the precursor T-cell level in HIV infection.
Wakabayashi
Y
Y
Ishiyama
T
T
Sugimoto
M
M
Abe
S
S
Horie
S
S
Matsumoto
T
T
Hirose
S
S
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000328:Adult; D015483:HIV Antibodies / Q000276:immunology; D006716:Homosexuality; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*
D015483:HIV Antibodies