Evidence for oxidative thiolytic cleavage of acetoin in Pelobacter carbinolicus analogous to aerobic oxidative decarboxylation of pyruvate.
Dihydrolipoamide dehydrogenase and dihydrolipoamide acetyltransferase were formed when Pelobacter carbinolicus strain GraBd1 was grown on acetoin. The specific activities of these enzymes amounted to 0.50 and 28.7 U/mg protein, respectively. The crude extract catalyzed the CoASH- and NAD+-dependent formation of acetyl-CoA from acetoin and methylacetoin. From ethylene glycol-grown cells these activities were absent. Crude extracts also exhibited acetoin: methyl viologen and acetoin: metronidazole oxidoreductase activity. As shown by reconstitution experiments methylviologen reduction was dependent on the presence of a light-brownish protein (Mr 220,000 +/- 10,000); metronidazole reduction was in addition dependent on the presence of a dark-brownish protein (Mr 4,900 +/- 800), which is probably a ferredoxin. However, both components were synthesized constitutively. We discussed a model for oxidative-thiolytic cleavage of acetoin which is analogous to the reaction of the pyruvate dehydrogenase enzyme complex rather than to pyruvate: ferredoxin oxidoreductase.
Oppermann
F B
FB
Institut für Mikrobiologie der Georg-August-Universität zu Göttingen, F.R.G.
Steinbüchel
A
A
Schlegel
H G
HG
D000093:Acetoin / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000096:biosynthesis; D000123:Acetyltransferases / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D002074:Butanones / Q000378:metabolism*; D002845:Chromatography; D003653:Decarboxylation; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D004591:Electrophoresis, Polyacrylamide Gel; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000378:metabolism; D008795:Metronidazole / Q000378:metabolism; D008956:Models, Chemical; D010084:Oxidation-Reduction; D011768:Pyruvate Dehydrogenase Complex; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid
D002074:Butanones; D005026:Ethylene Glycols; D011768:Pyruvate Dehydrogenase Complex; D011773:Pyruvates; D008795:Metronidazole; D000105:Acetyl Coenzyme A; D019289:Pyruvic Acid; D000093:Acetoin; D008058:Dihydrolipoamide Dehydrogenase; D000123:Acetyltransferases; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D019855:Ethylene Glycol