title: CML-T1: a cell line derived from T-lymphocyte acute phase of chronic myelogenous leukemia.
abstract: Most data suggest that malignant transformation in chronic myelogenous leukemia (CML) occurs in hematopoietic stem cell that is the progenitor of myelopoiesis and of B but not T lymphopoiesis. We established a T-lymphoid cell line (CML-T1) from a person with Ph-chromosome-negative CML in acute phase. Evidence of its T-lymphocyte origin includes the pattern cytochemical reactivity, reactivity with anti-T-cell monoclonal antibodies (MoAbs), and rearrangement of the beta-T-cell receptor (TCRB) gene. CML-T1 cells have features of type IV thymocytes. Cytogenetic analyses indicate a 47,XX, del(11), t(6;7)(q23;q24), +mar karyotype. CML-T1 cells exhibit molecular changes typical of CML, including translocation of the ABL protooncogene from chromosome 9 to 22, rearrangement of the BCR gene, and transcription of a chimeric BCR-ABL messenger RNA (mRNA). The ABL insertion on chromosome 22 appears interstitial, similar to other cases of Ph-chromosome-negative CML. These data clearly indicate that T cells can be involved in acute-phase CML. CML-T1 should be useful in studying this process as well as that underlying Ph-chromosome-negative CML.
authors: Department of Hematology, Nagasaki University School of Medicine, Japan.
authors: K
authors: K
authors: Kuriyama
authors: R P
authors: RP
authors: Gale
authors: M
authors: M
authors: Tomonaga
authors: S
authors: S
authors: Ikeda
authors: E
authors: E
authors: Yao
authors: I
authors: I
authors: Klisak
authors: K
authors: K
authors: Whelan
authors: H
authors: H
authors: Yakir
authors: M
authors: M
authors: Ichimaru
authors: R S
authors: RS
authors: Sparkes
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000032:analysis / Q000235:genetics / Q000473:pathology; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte; D015368:Human T-lymphotropic virus 1 / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor