Direct mutation analysis of beta-thalassemia genes in families of various ethnic origins residing in Germany.
DNA from Mediterranean and Asian beta-thalassemia patients, now residing in Germany, has been characterized by oligonucleotide hybridization and direct restriction analysis. Using five oligonucleotide pairs complementary to the most frequent beta-thalassemia mutations, and three different restriction enzymes, we were able to detect 33 of 36 mutations directly.
Schnee
J
J
Institut für Humangenetik der Universität, Münster, Federal Republic of Germany.
Eigel
A
A
Horst
J
J
D005787:Gene Frequency; D005914:Globins / Q000235:genetics*; D006801:Humans; D007438:Introns; D008522:Mediterranean Sea; D009154:Mutation; D015345:Oligonucleotide Probes; D010375:Pedigree; D013789:Thalassemia / Q000175:diagnosis / Q000235:genetics*
D015345:Oligonucleotide Probes; D005914:Globins