Recombinant vaccinia virus expressing the herpes simplex virus type 1 glycoprotein C protects mice against herpes simplex virus challenge.
The gene encoding the herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) was isolated and cloned into a vaccinia virus insertion vector, and the resulting vaccinia-gC vector was used to construct a recombinant vaccinia virus that expressed gC (VVgC5). Infection of cells with VVgC5 resulted in cell surface expression of authentic HSV-1 gC. HSV-1 gC-specific neutralizing antibodies were produced in VVgC5-immunized mice, and lymphocytes exhibited an HSV-1-specific proliferation response in vitro following infection. More importantly, VVgC5-immunized mice were resistant to subsequent lethal HSV-1 challenge.
Weir
J P
JP
Department of Microbiology, University of Tennessee, Knoxville 37996.
Bennett
M
M
Allen
E M
EM
Elkins
K L
KL
Martin
S
S
Rouse
B T
BT
D000818:Animals; D003001:Cloning, Molecular; D005814:Genes, Viral; D006561:Herpes Simplex / Q000517:prevention & control*; D008213:Lymphocyte Activation; D051379:Mice; D008970:Molecular Weight; D011233:Precipitin Tests; D015183:Restriction Mapping; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014616:Vaccinia virus; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000276:immunology*
D014612:Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines