title: Studies of lectin binding to the human cervix uteri: I. Normal cervix.
abstract: Lectins of Bauhinia purpurea (BPA), Canavalin ensiformis (Con A), Griffonia simplicifolia I (GS I), Griffonia simplicifolia II (GS II), Maclura pomifera (MPA), Arachis hypogaea (PNA), Glycine max (SBA), Ulex europaeus I (UEA I) and Triticum vulgaris (WGA) were used to evaluate cell surface carbohydrates in formalin-fixed paraffin-embedded tissue sections of normal human cervix uteri. Consistent patterns of staining of the squamous epithelium were obtained in all 30 cases with BPA, GS II, MPA, PNA, SBA and WGA. A variable distribution of lectin binding was seen in squamous epithelium with Con A, GS I and UEA I. The patterns of GS I and GS II binding reflected squamous epithelial maturation. Columnar epithelium did not stain with GS II, stained variably with Con A, and stained consistently with the remaining seven lectins in all cases. No association between lectin binding and blood group or phase of the menstrual cycle was found. These findings may be used as a baseline for evaluation of lectin binding in both preinvasive and invasive lesions of the cervix uteri.
authors: Department of Obstetrics and Gynaecology, Birmingham and Midland Hospital for Women, Sparkhill, UK.
authors: P
authors: P
authors: Byrne
authors: A
authors: A
authors: Williams
authors: T
authors: T
authors: Rollason
mesh_terms: D000328:Adult; D001789:Blood Group Antigens; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D002584:Cervix Uteri / Q000032:analysis* / Q000166:cytology / Q000378:metabolism; D004730:Endothelium, Vascular / Q000032:analysis / Q000166:cytology; D004848:Epithelium / Q000032:analysis / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008597:Menstrual Cycle; D009439:Neuraminidase / Q000378:metabolism; D012016:Reference Values
chemical_list: D001789:Blood Group Antigens; D002241:Carbohydrates; D037102:Lectins; D009439:Neuraminidase