A tubulin identified by a monoclonal antibody is not present in mitotic spindles.
A monoclonal antibody, G8, which recognizes a form of tubulin (G8-tubulin) with a novel distribution in Rat-1 cells and Potorous tridactylis kidney (Ptk-2) cells was isolated. G8 labeled the interphase cytoskeleton of Rat-1 fibroblasts but not mitotic spindles or midbodies. G8 also stained a fiber network in some but not all Ptk-2 interphase cells but did not label mitotic spindles or midbodies in these cells. G8-tubulin is the only identified tubulin known to be absent from these structures. This distribution may indicate that G8-tubulin possesses functional specificity.
Gossels
J M
JM
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Ingram
V M
VM
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D003599:Cytoskeleton / Q000032:analysis / Q000648:ultrastructure; D005347:Fibroblasts / Q000032:analysis / Q000166:cytology / Q000648:ultrastructure; D007399:Interphase; D007668:Kidney / Q000032:analysis / Q000166:cytology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D008941:Spindle Apparatus / Q000032:analysis* / Q000648:ultrastructure; D014404:Tubulin / Q000032:analysis* / Q000502:physiology
D000911:Antibodies, Monoclonal; D014404:Tubulin