[Increase in the in vitro activity of mollicute transcriptase using substances of an organic nature].
It is established that the use of DNA-dependent RNA-polymerases of mollicutes in the molecular-biological and gene-engineering works aimed to obtain a sufficient amount of the transcription products may promote a 1.5-2-fold increase of the yield by introducing such substances as glycerol, dimethylsulphoxide and spermidine (optimal concentrations: 10-12 vol. % and 12 mM, respectively) into the composition of the reacting mixture.
Skripal'
I G
IG
Babichev
V V
VV
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology*; D009174:Mycoplasma / Q000187:drug effects / Q000201:enzymology*; D011073:Polyamines / Q000494:pharmacology*
D011073:Polyamines; D012321:DNA-Directed RNA Polymerases; D005990:Glycerol; D004121:Dimethyl Sulfoxide
Povyshenie in vitro aktivnosti transkriptaz mollikutov pri pomoshchi veshchestv organicheskoĭ prirody.