Monoclonal antibodies for coagglutination of Streptococcus suis type 1.
Monoclonal antibodies (MAbs) were sought that would identify Streptococcus suis. Spleen cells from Balb/c mice immunized with formalin-killed S. suis were fused with the murine cell-line SP-2/0. Hybridomas positive for S. suis were identified by a solid-phase radioimmunoassay with a mixture of all available serotypes of S. suis. On further screening with individual serotypes of S. suis (Types 1, 2, 1/2, 3, 4, 5, 7, 8, 9 and 12) and other related and unrelated bacteria, a hybridoma specific for Type 1 was identified. Another hybridoma was positive for all serotypes of S. suis. S. suis Type 1-specific MAbs in the form of ascites fluid was used in coagglutination test. Agglutination with S. suis Type 1 was observed within 45 s, while there was no reaction with other serotypes of S. suis or other bacteria, during the 5-min observation period. A library of type-specific MAbs should help in rapid and accurate serotyping of S. suis isolates.
Payvandi
F
F
Department of Veterinary Science, University of Nebraska, Lincoln 68583-0905.
Srikumaran
S
S
Shields
T R
TR
Erickson
E D
ED
D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007132:Immunoglobulin Isotypes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay; D012703:Serotyping; D013205:Staphylococcal Protein A / Q000276:immunology; D013291:Streptococcus / Q000145:classification* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes; D013205:Staphylococcal Protein A