High-performance liquid chromatographic determination of ibuprofen, its metabolites and enantiomers in biological fluids.
An isocratic high-performance liquid chromatographic method to determine racemic ibuprofen (assay I) and its major metabolites (assay II) in biological fluids (plasma, urine, bile) using a conventional reversed-phase column is described. A third assay using beta-cyclodextrin as stationary phase (Cyclobond I) for the separation of the ibuprofen enantiomers is also described. A wavelength of 220 nm was used to monitor the substances. The sensitivity of the method was 0.1 microgram/ml for all three assays. The method was demonstrated to be suitable for stereoselective pharmacokinetic studies of ibuprofen in humans and animals.
Geisslinger
G
G
Department of Pharmacology and Toxicology, University of Erlangen-Nürnberg, F.R.G.
Dietzel
K
K
Loew
D
D
Schuster
O
O
Rau
G
G
Lachmann
G
G
Brune
K
K
D001646:Bile / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007052:Ibuprofen / Q000097:blood / Q000378:metabolism* / Q000652:urine; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D007052:Ibuprofen