Evaluation of the polyethylene glycol-indirect antiglobulin test for routine compatibility testing.
All specimens received in the blood bank over a 5-month period for crossmatch or group and screen requests were tested in parallel by a polyethylene glycol-indirect antiglobulin test (PEG-IAT) and a low-ionic-strength saline (LISS)-IAT. The sera of 41 of 1471 patients had reactions, with 50 antibodies being detected. Ten antibodies reacted only on the PEG-IAT and 14 only by the LISS-IAT; the remaining 26 antibodies were detected by both methods. Of the antibodies that reacted only by the LISS-IAT, one (anti-Jka) was considered clinically significant, whereas five of the antibodies that reacted only by the PEG-IAT (1 anti-c, 2-Fya, 1-Jkb, and 1-S) were considered significant. Two antibodies of questionable clinical significance were detected only by the PEG-IAT. In 97 percent of the sera tested, no reaction was detected by either method. The PEG-IAT is an acceptable technique for routine compatibility testing.
Slater
J L
JL
Regional Laboratory Services, Royal Columbian Hospital, New Westminster, British Columbia, Canada.
Griswold
D J
DJ
Wojtyniak
L S
LS
Reisling
M J
MJ
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D003298:Coombs Test; D006801:Humans; D011092:Polyethylene Glycols
D011092:Polyethylene Glycols