title: Otitis media in children: local immune response to nontypeable Haemophilus influenzae.
abstract: Twenty children experienced 30 episodes of otitis media with effusion due to nontypeable (NT) Haemophilus influenzae in the first 2 years of life. The local and systemic immune responses to homologous strains of NT H. influenzae were determined by an immunodot assay. Strain-specific immunoglobulin G (IgG) antibody predominated in the middle ear fluid (MEF). It was detected in 91% of the children, compared with IgM in 48% (P less than 0.005), IgA in 52% (P less than 0.005), and secretory IgA in 18% (P less than 0.005). The titer (log2) of NT H. influenzae-specific IgG antibody (mean +/- standard error, 8.2 +/- 0.1) exceeded the titers of IgM (3.4 +/- 0.1), IgA (3.7 +/- 0.1), and secretory IgA (1.2 +/- 0.3). NT H. influenzae-specific antibody was detected exclusively in MEFs of individuals who possessed homologous serum antibody. Although antibody titers in MEF declined over time, serum antibody titers remained stable. These data suggest that immunity to NT H. influenzae in the middle ear, in part, reflects systemic immunity. Whereas local antibody disappears after resolution of the infection, systemic antibody persists.
authors: Department of Pediatrics and Otolaryngology, School of Medicine, State University of New York at Buffalo 14222.
authors: H
authors: H
authors: Faden
authors: L
authors: L
authors: Brodsky
authors: J
authors: J
authors: Bernstein
authors: J
authors: J
authors: Stanievich
authors: D
authors: D
authors: Krystofik
authors: C
authors: C
authors: Shuff
authors: J J
authors: JJ
authors: Hong
authors: P L
authors: PL
authors: Ogra
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D004432:Ear, Middle / Q000276:immunology; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007223:Infant; D010033:Otitis Media / Q000276:immunology* / Q000382:microbiology; D011446:Prospective Studies; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M