Lipoperoxidation and T-cell leukemia of childhood. Effects of indomethacin.
Serum thiobarbituric acid (TBA) reactivity for lipoperoxidation products was assessed at diagnosis in children with T-cell and common acute lymphocytic leukemia (ALL) and T-lymphoblastic lymphoma. Comparisons were made among these groups and with healthy controls. Mean TBA reactivity (mumol malondialdehyde/L serum) was increased (P less than 0.01) in the T-cell leukemia group versus common ALL and T-lymphoblastic lymphoma patients and controls, respectively. The increase in lipoperoxidation products in T-cell ALL appeared to bear a positive relation to peripheral leukocyte counts, and was accompanied by increased serum prostaglandin E2 (PGE2) levels in most representative cases. Indomethacin added to a childhood T-cell ALL line (SUP-T3), at a concentration known to inhibit prostaglandin synthesis in vitro (i.e., 3 micrograms/mL), effected significant increases in the numbers of natural killer (NK; Leu-11+ and Leu-19+) cells (P less than 0.01) and B-lymphocytes (P less than 0.05), and significant decreases in cell viability (P less than 0.01). Indomethacin may be a useful agent for enhancing the antileukemic immune response in T-cell ALL.
Brown
R E
RE
Department of Pathology, Cook-Fort Worth Children's Medical Center, Forth Worth, Texas 76104.
Alade
S L
SL
Wu
M C
MC
Wu
F M
FM
Bowman
W P
WP
D002648:Child; D015232:Dinoprostone / Q000378:metabolism; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000378:metabolism*; D007958:Leukocyte Count; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D013858:Thiobarbiturates / Q000097:blood; D014407:Tumor Cells, Cultured
D013858:Thiobarbiturates; D015232:Dinoprostone; C029684:thiobarbituric acid; D007213:Indomethacin