[Survival of 4-cell mouse embryos derived from in vitro fertilization after ultrarapid freezing and thawing].
Four-cell mouse embryos obtained by fertilization in vitro were frozen ultrarapidly, immediately after one-step exposure to a highly concentrated solution (modified VS1) at room temperature, and later thawed in a 37 degrees C waterbath. The proportion of morphologically normal embryos on thawing was 95.7% (198/207), and 83.8% (166/198) of these embryos developed to morulas and early blastocysts after 24 hr in culture. All frozen-thawed embryos that developed to morulas and early blastocysts were transferred to the uterine horns of recipients on day 3 of pseudopregnancy and 47.0% (78/166) of transferred embryos developed to normal young.
Nakagata
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Central Laboratory for Medical Sciences, School of Medicine, Juntendo University, Japan.
D000818:Animals; D002470:Cell Survival; D005314:Embryonic and Fetal Development; D005307:Fertilization in Vitro; D005615:Freezing; D051379:Mice; D008815:Mice, Inbred Strains; D011309:Preservation, Biological