title: Interleukin-1 beta rather than lymphotoxin as the major bone resorbing activity in human multiple myeloma.
abstract: Human myeloma cells were purified from bone marrow aspirates from four patients having advanced myeloma, including one with common acute lymphoblastic leukemia antigen-positive myeloma. All of these myelomas had marked bone lytic lesions. From the culture supernatants of these purified myeloma cells, bone-resorbing activities were significantly revealed by 45Ca-release bone resorption assay, and IL-1 activities were also detected by IL-1 bioassay (mouse thymocyte comitogenic assay). Sandwich enzyme immunoassay for IL-1 alpha or IL-1 beta revealed that IL-1 beta was responsible for IL-1 activity of these culture supernatants. Furthermore, the bone resorbing activities of these culture supernatants were completely neutralized by pretreatment of anti-IL-1 beta, but not anti-IL-1 alpha antibody. By Northern blot analysis, IL-1 beta mRNA was identified from these myeloma cells. Therefore, it is concluded that myeloma cells produce IL-1 beta, which acts as bone-resorbing activity in multiple myeloma.
authors: Department of Internal Medicine, Hiroshima University, Japan.
authors: M
authors: M
authors: Kawano
authors: I
authors: I
authors: Yamamoto
authors: K
authors: K
authors: Iwato
authors: H
authors: H
authors: Tanaka
authors: H
authors: H
authors: Asaoku
authors: O
authors: O
authors: Tanabe
authors: H
authors: H
authors: Ishikawa
authors: M
authors: M
authors: Nobuyoshi
authors: Y
authors: Y
authors: Ohmoto
authors: Y
authors: Y
authors: Hirai
mesh_terms: D000937:Antigen-Antibody Reactions; D001862:Bone Resorption; D002478:Cells, Cultured; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000235:genetics / Q000502:physiology*; D008233:Lymphotoxin-alpha / Q000502:physiology*; D009101:Multiple Myeloma / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D007375:Interleukin-1; D008233:Lymphotoxin-alpha; D012333:RNA, Messenger