Characterization of the antigen-reactive T cell line mediating in vivo delayed type hypersensitivity established by antigen-induced interleukin 2.
The murine antigen-reactive T cell line has been established by antigen-induced interleukin 2 (IL-2) derived from immunized syngeneic mice. The surface markers of the cells showed Thy 1+, and Lyt 1+2-. The antigen-reactive cells require the presence of IL-2 for continuous proliferation. IL-2 from mouse, rat, or human sources all stimulate the T cell line growth. The T cell mitogen such as concanavalin A and antigen alone do not stimulate cell proliferation of the cells. The cells proliferate antigen-specifically in the presence of both IL-2 and antigen-presenting cells from histocompatible mice. Antigen-specific in vivo delayed type hypersensitivity response is inducible by injection of the T cells into naive syngeneic mice along with IL-2 and antigen. The reactivity of the T cell line found in vitro (proliferation) and in vivo (delayed type hypersensitivity) is highly antigen-specific, because the cells do not respond another antigen used for immunizing mice. The antigen-reactive T cell line produces neither IL-2 nor inhibiting factors such as neutralizing factors against preformed IL-2 activity and IL-2 production inhibiting factors, thus the cells are exclusive IL-2 acceptor. These results suggest that the interaction of antigen-induced IL-2 and IL-2 dependent antigen-reactive T cells may play a significant role in the induction/expression of in vivo delayed type hypersensitivity to specific antigen, because antigen-induced IL-2 is probably only a relevant IL-2 in the immunized mice.
Kobayashi
K
K
First Department of Internal Medicine, Showa University School of Medicine, Tokyo, Japan.
Cohen
S
S
Yoshida
T
T
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D007376:Interleukin-2