[Immunohistochemical localization and significance of ornithine aminotransferase in gut and liver in rats].
This study was performed to elucidate immunohistochemical localization of OAT in the gut mucosa and liver of rats using the ABC method. In the normal rat gut mucosa, strong immunoreactivity of OAT as fine granules was present in the surface epithelial cells and apical portion of intestinal glands. In the liver, this reaction was observed in the range of zone 2 to 3 of the pericentral vein and periportal vein. The changes in the intestinal glands of rats with a hyperprotein intake were observed to peak at 2 weeks, but in the liver a strongly positive diffuse reaction throughout whole lobules was detected at 4 weeks. This immunoreactive morphological localization of OAT suggests the location of absorption of amino acids in the small intestine and the distribution of the urea cycle enzyme OAT in the liver. The relationship between absorption of amino acids in the small intestine and the distribution of OAT in the liver was assumed to facilitate feedback repression. The changes in OAT in the mitochondria showed many diverse patterns by immunochemical electron microscopy, and these reactions are presumed to facilitate various types of metabolism in a single cells.
Tashiro
K
K
Kasahara
M
M
Kamei
K
K
Terashima
Y
Y
D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000201:enzymology; D007422:Intestines / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009953:Ornithine-Oxo-Acid Transaminase / Q000032:analysis*; D051381:Rats; D011921:Rats, Inbred WKY; D000637:Transaminases / Q000032:analysis*
D004044:Dietary Proteins; D000637:Transaminases; D009953:Ornithine-Oxo-Acid Transaminase