Spectrotypic analysis of humoral response in human hydatidosis.
The spectrotypic patterns of anti-Echinococcus granulosus and anti-F(ab')2 antibodies were analysed in 13 patients with different forms of hydatidosis by means of isoelectric focusing (IEF). Specific anti-echinococcus antibodies and specific echinococcus antigens were obtained by crossed affinity chromatography between patient serum and cyst fluid, as previously described (D'Amelio et al., 1985). A wide spectrotype in the pattern of anti-Echinococcus antibodies was observed, with strong homologies among the different sera mainly at acid and neutral pH. One serum displayed faint, nearly inapparent bands. Conversely, the pattern of anti-F(ab')2 antibodies in the six patients in whom they were positive was limited but heterogeneous, ranging from monoclonal through oligoclonal to polyclonal. A predominant clone was identified in the patient with a polyclonal pattern. A correlation between qualitative IEF spectrotypic patterns and the quantitative results of immunoenzymatic assays was observed for both types of antibodies.
D'Amelio
R
R
Department of Pathology Centre Médical Universitaire, Geneve, Switzerland.
Del Giudice
G
G
De Rosa
F
F
Brighouse
G
G
Teggi
A
A
Lambert
P H
PH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000909:Antibodies, Helminth / Q000032:analysis*; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology*; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged
D000888:Antibodies, Anti-Idiotypic; D000909:Antibodies, Helminth; D007140:Immunoglobulin Fab Fragments