An analysis of serotonin secretion in hypothalamic regions based on 5-hydroxytryptophan accumulation or push-pull perfusion. Effects of mesencephalic raphe or locus coeruleus stimulation and correlated changes in plasma luteinizing hormone.
This study evaluates the effect of electrical stimulation (ES) of the dorsal (DRN) and median raphe (MRN) nuclei serotoninergic systems on luteinizing hormone (LH) release in estrogen-treated, ovariectomized rats. To show that ES increased serotonin (5-hydroxytryptamine: 5-HT) secretion into hypothalamic regions known to contain luteinizing hormone-releasing hormone (LH-RH) cell bodies and terminals. 5-hydroxytryptophan (5-HTP) accumulation was measured in microdissected hypothalamic areas after blockade of aromatic-L-amino-acid decarboxylase with NSD-1015. DRN-ES produced a significant increase in 5-HTP accumulation in the medial preoptic (MPN) and paraventricular nuclei (PVN), but not in the suprachiasmatic nucleus (SCN), arcuate nucleus (ARC) or median eminence (ME). In contrast, MRN-ES produced a significant rise in 5-HTP accumulation only in the PVN and ARC, not in the SCN, MPN or ME. Because the DRN receives noradrenergic innervation from the locus coeruleus (LC) we also evaluated the effect of LC stimulation on 5-HT secretion into these hypothalamic regions. LC stimulation, like DRN-ES, resulted in increases in 5-HTP accumulation in MPN and PVN, but not in SCN or ME. In addition, using push-pull perfusion methodology, we observed that LC-ES results in a 240% increase in 5-HT and a decrease of approximately 40% in 5-hydroxyindoleacetic acid (5-HIAA) in MPN perfusates collected 10-20 min after LC-ES began. With evidence that DRN- and MRN-ES evoke 5-HT release, we next examined whether such stimulation affects basal LH release. Because we were unable to find any effect, we determined whether 5-HT release would augment or suppress electrochemically evoked LH release. MPN electrochemical stimulation (ECS) induced a significant increase in plasma LH with a peak seen at 45 min. When the MPN was ECS and 30 min later the DRN was ES for 30 min no augmenting or suppressive effect was seen during the first 60 min. However, while plasma LH declined towards baseline in the MPN-ECS group, it remained significantly elevated in MPN-ECS + DRN-ES rats. MPN-ECS + MRN-ES had no such effect: instead. LH levels were transiently decreased 45 min after beginning MPN-ECS. This study provides additional information on hypothalamic sites which receive axonal projections from the DRN and MRN and clearly indicate that an increase in 5-HT secretion occurs in the MPN following DRN- or LC-ES.(ABSTRACT TRUNCATED AT 400 WORDS)
Petersen
S L
SL
Department of Physiology, School of Medicine, University of Maryland, Baltimore 21201.
Hartman
R D
RD
Barraclough
C A
CA
D006916:5-Hydroxytryptophan / Q000378:metabolism*; D000818:Animals; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006834:Hydrazines / Q000494:pharmacology; D007031:Hypothalamus / Q000378:metabolism*; D008125:Locus Coeruleus / Q000502:physiology*; D007986:Luteinizing Hormone / Q000097:blood*; D010052:Ovariectomy; D011903:Raphe Nuclei / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D004791:Enzyme Inhibitors; D006834:Hydrazines; D012701:Serotonin; D007986:Luteinizing Hormone; C004297:3-hydroxybenzylhydrazine; D006916:5-Hydroxytryptophan