[The effect of 3,4-disuccinyldianhydrogalactitol, N-nitrosourea and their combination on DNA synthesis in normal and mouse P388 tumor cells].
The rates of incorporation of 2-14C-thymidine into DNA of leukemia P388, bone marrow, gastrointestinal mucosa and spleen cells at various time after administration of 3,4-disuccinyldianhydrogalactitol (DisuDAG), 1-methyl-1-nitrosourea (MNU), 1-(2-hydroxyethyl)-3-(2-chloroethyl)-3-nitrosourea (HECNU) and their combinations at different doses to mice with leukemia P388 (solid form) were studied. DisuDAG (80 mg/kg) induced the deep and the stable inhibition in DNA synthesis of leukemia P388, bone marrow and spleen cells. The combination of DisuDAG and HECNU at small doses induced the deep and the stable suppression of DNA synthesis in tumor cells, however DNA synthesis in normal dividing cells was shown to recover more rapidly than in leukemia P388 cells. Administration of the combination of DisuDAG with MNU to tumor-bearing mice induced more stable inhibition of DNA synthesis in tumor cells in comparison with MNU and DisuDAG. In vivo inhibition of DNA synthesis in leukemia P388 cells with DisuDAG and HECNU was not due to damage in pool of precursors (TCA soluble fraction).
Sokolova
I S
IS
Veres
Z
Z
Jakus
J
J
Otvos
L L
LL
Gorbacheva
L B
LB
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects* / Q000378:metabolism; D003961:Dianhydrogalactitol / Q000031:analogs & derivatives / Q000494:pharmacology*; D004357:Drug Synergism; D007413:Intestinal Mucosa / Q000187:drug effects; D007941:Leukemia P388 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D008770:Methylnitrosourea / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D009607:Nitrosourea Compounds / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects; D013402:Sugar Alcohols / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D009607:Nitrosourea Compounds; D013402:Sugar Alcohols; D003961:Dianhydrogalactitol; C044246:3,4-disuccinyl 1,2-5,6-dianhydrogalactitol; D008770:Methylnitrosourea; D004247:DNA; C013876:elmustine
Vliianie 3,4-disuktsinildiangidrogalaktitola, N-nitrozomochevin i ikh kombinatsiĭ na sintez DNK v normal'nykh i opukholevykh kletkakh mysheĭ s leĭkozom P388.