Chromosome aberrations induced by pyrolysates of carbohydrates in Chinese hamster V79 cells.
The clastogenic activity of some pyrolysates of carbohydrates was examined in cultured Chinese hamster V79 cells. These pyrolysates include levoglucosan (LG-I), levoglucosenone (LG-II), furfural (FF), 5-(hydroxymethyl)-2-furfural (HMF), glyoxal (GL), methylglyoxal (MGL), 3-deoxy-D-glucosone (DG) and thiazolidine (TZ). LG-I did not induce a significant number of chromosome aberrations at doses up to 8000 micrograms/ml. In contrast, the related compound LG-II induced aberrations and reduced mitosis in a dose-dependent fashion at around 1/2000 of the LG-I doses. Both furan derivatives, FF and HMF, and both glyoxal derivatives, GL and MGL, induced a significant number of chromosome aberrations and a significant lowering of mitotic activity. Among these compounds, FF and MGL showed stronger clastogenic activity than HMF and GL, respectively. DG slightly but positively induced chromosome aberrations. TZ was one of the most potent clastogens among the compounds examined in this study, showing the highest incidence of aberrant cells with many exchanges at doses inducing a significant lowering of mitotic activity. The results of this study indicate the need for a re-evaluation of the thermal decomposition of carbohydrates as a source of genotoxic contaminants.
Nishi
Y
Y
Life Science Research Laboratory, Japan Tobacco Inc., Kanagawa.
Miyakawa
Y
Y
Kato
K
K
D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000633:toxicity*; D002241:Carbohydrates / Q000633:toxicity*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D005947:Glucose / Q000031:analogs & derivatives* / Q000633:toxicity; D006358:Hot Temperature; D015416:Maillard Reaction; D009153:Mutagens
D019086:Bridged Bicyclo Compounds, Heterocyclic; D002241:Carbohydrates; D009153:Mutagens; C512757:1,6-anhydro-3,4-dideoxyhex-3-enopyran-2-ulose; D005947:Glucose