Expert system computer program for troubleshooting the gel-clot Limulus amebocyte lysate (LAL) assay.
This article describes a knowledge-based or expert system computer program that can assist in troubleshooting the gel-clot Limulus Amebocyte Lysate (LAL) method for the assay of endotoxin in parenteral products and in-process materials. The program may also be used to train new personnel and/or to assist in the development of an LAL method for a new product or material. The program includes LAL assay information for 31 parenteral products and materials. The program uses rules-of-thumb and intuition to diagnose 155 possible LAL assay problems and logically reduce them to 37 recommended problem solving processes. The program also provides references to support its recommendation.
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D004731:Endotoxins / Q000032:analysis; D005103:Expert Systems; D008033:Limulus Test; D012984:Software
D004731:Endotoxins