Obligatory role of B cells and adherent accessory cells in the transfer of a defect in morphine-mediated antinociception in C57BL/6J-bg/bg (beige-J) mice.
C57BL/6J-bg/bg (beige-J) mice have a blunted antinociceptive response to intracerebroventricularly (i.c.v.) injected morphine in the tail-flick test. Beige-J mice are also immunologically defective and exhibit the pathology of Chediak-Higashi syndrome (CHS). We transferred by i.v. injection 2 x 10(7) mononuclear spleen cells, devoid of PMNs, obtained from beige-J mice to normal C57BL/6J-bg/+ littermates that do not exhibit CHS or a blunted antinociceptive response to morphine. After 8 days, the normal littermates demonstrated significant (P less than 0.05) reduction in their analgesic responsiveness to morphine. This phenomenon was found to require B-cells and adherent cells in the adoptively transferred spleen cells. B-cells that had been purified by panning on anti-Ig-coated plates were sufficient to transfer the analgesic defect unless adherent cells were removed prior to immunocytoadherence. T-cells, in the presence or absence of adherent cells, failed to transfer the decreased sensitivity to morphine. These results demonstrate a novel neuroimmune interaction whereby B-lymphocytes and adherent cells, or a substance derived from them, are able to affect the antinociceptive action of morphine.
Kimball
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Department of Biological Research, Janssen Research Foundation, Spring House, PA 19477.
Raffa
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D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology* / Q000637:transplantation; D002448:Cell Adhesion; D004351:Drug Resistance; D007116:Immunization, Passive; D007375:Interleukin-1 / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D009000:Monocytes / Q000378:metabolism; D009020:Morphine / Q000494:pharmacology*; D009154:Mutation; D009619:Nociceptors / Q000502:physiology*; D010969:Plastics; D013154:Spleen / Q000166:cytology / Q000637:transplantation
D007375:Interleukin-1; D010969:Plastics; D009020:Morphine