1-Oleoyl-2-acetylglycerol promotes immunoglobulin production independent of cell proliferation in human peripheral blood mononuclear cells.
1-Oleoyl-2-acetylglycerol (OAG) stimulated IgG and IgM production in a dose-dependent manner in human peripheral blood mononuclear cells (PBM) but not PBM proliferation. 12-O-Tetradecanoyl phorbol-13-acetate (TPA) did not stimulate Ig production. OAG did not stimulate an increase in IL-2 generation or IL-2 receptor expression. H-7, a protein kinase C blocker completely inhibited OAG-stimulated Ig production. The results suggest that OAG stimulation of Ig production is independent of cell proliferation; a generalized increase in T-cell activation does not appear to be necessary in the OAG stimulation of Ig production. Finally, PBMs respond differently to OAG and TPA although both are protein kinase C activators.
Yamaguchi
D T
DT
Research Service, VA Medical Center, West Los Angeles, CA.
Sakai
R
R
Jordan
S C
SC
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000917:Antibody Formation / Q000187:drug effects; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004075:Diglycerides / Q000494:pharmacology*; D005989:Glycerides / Q000494:pharmacology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis; D007546:Isoquinolines / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis
D004075:Diglycerides; D005989:Glycerides; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007376:Interleukin-2; D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D015375:Receptors, Interleukin-2; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C038237:1-oleoyl-2-acetylglycerol