Coexistence of delta sleep-inducing peptide and serotonin in midgut carcinoid tumour cells in vivo and in vitro.
By immunocytochemistry, delta sleep-inducing peptide (DSIP) was demonstrated to coexist with serotonin (5-HT) in a majority of midgut carcinoid tumour cells studied in biopsies and long-term cell cultures. Tumour cell colonies were characterized ultrastructurally and by confocal laser microscopy. The cultures produced several DSIP-like peptides chromatographically separated from culture media. DSIP has not yet proved to be a useful tumour marker clinically. Provocation with pentagastrin in patients with midgut carcinoid syndrome resulted in increased peripheral levels of 5HT, but not of DSIP.
Ahlman
H
H
Department of Surgery, University of Göteborg, Sweden.
Ahlund
L
L
Nilsson
O
O
Dahlström
A
A
Bjartell
A
A
Ekman
R
R
D001706:Biopsy; D002276:Carcinoid Tumor / Q000378:metabolism* / Q000648:ultrastructure; D003701:Delta Sleep-Inducing Peptide / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007414:Intestinal Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D008198:Lymph Nodes / Q000378:metabolism / Q000648:ultrastructure; D008207:Lymphatic Metastasis; D010418:Pentagastrin; D012701:Serotonin / Q000032:analysis / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D012701:Serotonin; D003701:Delta Sleep-Inducing Peptide; D010418:Pentagastrin