Calcitonin gene-related peptide-like immunoreactivities in pheochromocytomas.
Calcitonin gene-related peptide (CGRP) is reported to exist in high concentrations in plasma and tumor tissues of medullary thyroid carcinomas. CGRP-like immunoreactivity (LI) in tumor tissues of pheochromocytomas was investigated by radioimmunoassay. CGRP-LI in 9 pheochromocytomas ranged from 0.50 to 1240 ng/g wet tissue. Sephadex G-50 column chromatography revealed that most of CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP. Reverse-phase high pressure liquid chromatography revealed that CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP and in a more hydrophobic position. These results indicate that high concentrations of CGRP-LI also exist in tumor tissues of pheochromocytomas.
Mouri
T
T
Second Department of Internal Medicine, Tohoku University School of Medicine, Miyagi, Japan.
Takahashi
K
K
Sone
M
M
Murakami
O
O
Ohneda
M
M
Itoi
K
K
Imai
Y
Y
Yoshinaga
K
K
Sasano
N
N
D000310:Adrenal Gland Neoplasms / Q000032:analysis*; D002116:Calcitonin / Q000032:analysis*; D015740:Calcitonin Gene-Related Peptide; D002850:Chromatography, Gel; D006801:Humans; D009479:Neuropeptides / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D010673:Pheochromocytoma / Q000032:analysis*; D011863:Radioimmunoassay
D009479:Neuropeptides; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide