title: Recycling of epidermal growth factor-receptor complexes in A431 cells.
abstract: The fate of epidermal growth factor (EGF) after internalization by human carcinoma A431 cells has been studied. Cells were allowed to internalize 125I-EGF for 10 min at 37 degrees C and treated with acid/salt solution to remove non-internalized ligand. Further incubation of these '125I-EGF-loaded' cells at 37 degrees C results in rapid recycling of internalized 125I-EGF-receptor complexes back to the cell surface. Recycling was assessed by measuring the increase in plasma membrane pool of 125I-EGF-receptor complexes as they became sensitive to acid/salt treatment, cross-linking with the membrane impermeant reagent bis(sulfosuccinimidyl)suberate and competitive substitution by unlabeled EGF. Moreover, redistribution of 125I-EGF-receptor complexes from endosomes to the plasma membrane was demonstrated using a subcellular fractionation technique. More than 50% of the total internalized EGF was found to be capable of recycling. The rate of recycling was significantly higher than that of EGF degradation in lysosomes. It was shown that EGF/receptor recycling is an energy-requiring and temperature-dependent process. Fluorescence microscopy studies demonstrate that endosomes located in a region adjacent to the Golgi complex are involved in the the recycling of EGF-receptor complexes in A431 cells. The data obtained suggest that dissociation of EGF from internalized receptor is not necessary for EGF receptor recycling.
authors: Institute of Cytology Academy of Sciences U.S.S.R., Leningrad.
authors: A
authors: A
authors: Sorkin
authors: E
authors: E
authors: Kornilova
authors: L
authors: L
authors: Teslenko
authors: A
authors: A
authors: Sorokin
authors: N
authors: N
authors: Nikolsky
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D003432:Cross-Linking Reagents; D004734:Energy Metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D005456:Fluorescent Dyes; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D051379:Mice; D008856:Microscopy, Fluorescence; D013388:Succinimides; D013696:Temperature; D014407:Tumor Cells, Cultured
chemical_list: D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D013388:Succinimides; D004815:Epidermal Growth Factor; C035760:bis(sulfosuccinimidyl)suberate; D066246:ErbB Receptors