Interleukin-2 (rIL-2)-induced lymphokine-activated killer (LAK) cells and their precursors express the VGO1 antigen.
Precursor and effector cells of recombinant interleukin-2 (r-IL-2)-induced lymphokine-activated killer (LAK) activity were investigated for their expression of VGO1. Peripheral blood lymphocytes (PBL) from normal donors were purified and separated in a FACS 420 into VGO1+- and VGO1- cell fractions before and after culture for 96 hr with 100 U/ml of r-IL-2. Their lytic activity against K 562 and Daudi cells was measured in a 51Cr release assay. The majority, if not all, of the LAK effector and precursor cells was VGO1+ lymphocytes. The expression of VGO1 by LAK precursor cells remained stable under the culture conditions used in our experiments. VGO1- lymphocytes cultured with r-IL-2 demonstrated neither LAK-induced activity nor expression of VGO1 antigen.
Denegri
J F
JF
Department of Pathology, Vancouver General Hospital, B.C., Canada.
Peterson
J
J
Tilley
P
P
D000954:Antigens, Surface / Q000096:biosynthesis*; D002469:Cell Separation; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013234:Stem Cells / Q000276:immunology*
D000954:Antigens, Surface; D002860:Chromium Radioisotopes; D007376:Interleukin-2; D011994:Recombinant Proteins