[Secretion of pectate lyase and protein composition of the outer membrane of Erwinia chrysanthemi ENA49 cells].
The combined changes (specters of isoenzymes of intracellular and extracellular pectatelyase, protein composition of periplasm and outer membrane) in the cells of E. chrysanthemi ENA49 from the periodical culture growing on the inducer containing medium have been studied. The beginning of active pectatelyase synthesis was established to be accompanied by the temporal intracellular accumulation of the enzyme. The cellular capability of pectatelyase secretion to the outer medium correlates with the presence of the definite protein in the outer membrane. The different pathways for secretion of pectatelyase isoenzymes PLb, PLc, PLd and PLe are suggested.
Shevchik
V E
VE
Evtushenkov
A N
AN
Fomichev
Iu K
IuK
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004885:Erwinia / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D007527:Isoenzymes; D011133:Polysaccharide-Lyases; C023911:pectate lyase
Sekretsiia pektatliazy i sostav belkov naruzhnoĭ membrany kletok Erwinia chrysanthemi ENA49.