Activation of normal human B cells through their antigen receptor induces membrane expression of IL-1 alpha and secretion of IL-1 beta.
We studied whether normal human B cells would express IL-1 activity and transcribe IL-1 genes before or after activation through their Ag receptor. Anti IgM antibody-activated B cells expressed IL-1 alpha on the cell surface and secreted IL-1 beta. Optimal induction of IL-1 occurred within 16 h of anti-IgM activation. mRNA specific for both IL-1 alpha and IL-1 beta was also induced upon activation. It is likely that the expression of m-IL-1 alpha on activated B cells together with the secretion of IL-1 beta represent important contributions in the efficient Ag-presenting capacity of B cells to T cells.
Bonnefoy
J Y
JY
UNICET, Laboratory for Immunological Research, Dardilly, France.
Denoroy
M C
MC
Guillot
O
O
Martens
C L
CL
Banchereau
J
J
D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D002648:Child; D005786:Gene Expression Regulation; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology / Q000502:physiology; D007375:Interleukin-1 / Q000096:biosynthesis / Q000145:classification / Q000378:metabolism*; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism*; D014158:Transcription, Genetic
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D007375:Interleukin-1; D008565:Membrane Proteins; C044203:anti-IgM