Depression of the immune responsiveness in mice treated with thioacetamide after antigen exposure.
The effects of the weak carcinogen thioacetamide (TAA) on the mouse immune response have been investigated. TAA administration up to 1 day after antigen priming markedly suppressed the antibody response to both T-dependent (sheep erythrocytes) and T-independent (trinitrophenyl-Ficoll) antigens; the compound was ineffective when given 3 or 4 days after immunization. A significant suppression of the in vitro lymphoproliferative response to the B-cell mitogen S. aureus Cowan I was evident from 3 to 48 h after TAA treatment. On the other hand, cell-mediated immune response to oxazolone was suppressed by TAA at each time tested, including that of challenge. The in vitro lymphoproliferative response to concanavalin A was decreased 12 h after TAA administration only, when adenosine deaminase activity within lymphocytes was increased. Furthermore, TAA is endowed with anti-inflammatory activity, as shown by the decreased footpad swelling and by evaluation of the inflammatory cell infiltration upon carrageenan injection. Taken together, these findings suggest selective TAA interaction with multiple targets within the immune system, including B- and T-lymphocytes, while non-specific cytotoxicity can be reasonably ruled out, since hematological determinations and phenotypic analysis of spleen lymphocyte subsets showed no relevant changes.
Malvaldi
G
G
Institute of General Pathology, University of Pisa, Italy.
Batoni
G
G
Marelli
P
P
Zolfino
I
I
Senesi
S
S
Danesi
R
R
Campa
M
M
D000081:Acetamides / Q000494:pharmacology*; D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000917:Antibody Formation / Q000187:drug effects; D000941:Antigens / Q000008:administration & dosage*; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects; D007166:Immunosuppressive Agents; D007249:Inflammation / Q000188:drug therapy; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D013853:Thioacetamide / Q000494:pharmacology*
D000081:Acetamides; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000941:Antigens; D007166:Immunosuppressive Agents; D013853:Thioacetamide; D000243:Adenosine Deaminase