Stimulation of ovine placental calcium transport by purified natural and recombinant parathyroid hormone-related protein (PTHrP) preparations.
Perfusion in situ of the placenta of previously thyroparathyroidectomized fetal lambs has been used to compare the ability of various forms of parathyroid hormone-related protein (PTHrP) to stimulate placental calcium transport. Whereas PTHrP (1-34) was without effect, PTHrP (1-141) was active but usually after a delay of up to 1 h, in common with the effect noted when using extracts of fetal parathyroid glands. In contrast, PTHrP (1-84) and PTHrP (1-108), tended to show a more rapid stimulatory action. It is suggested that post-translational processing of PTHrP (1-141) may occur as an activating step in the placenta in vivo.
Abbas
S K
SK
Department of Animal Physiology and Nutrition, University of Leeds.
Pickard
D W
DW
Rodda
C P
CP
Heath
J A
JA
Hammonds
R G
RG
Wood
W I
WI
Caple
I W
IW
Martin
T J
TJ
Care
A D
AD
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D009363:Neoplasm Proteins / Q000494:pharmacology*; D010281:Parathyroid Hormone / Q000032:analysis; D044162:Parathyroid Hormone-Related Protein; D010477:Perfusion; D010920:Placenta / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D012756:Sheep / Q000502:physiology; D013965:Thyroidectomy; D013997:Time Factors
D009363:Neoplasm Proteins; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D011994:Recombinant Proteins; D002118:Calcium