Metabolism of [14C]cholesterol to C-20 isomeric [14C]pregn-5-ene-3,20-diols in the tobacco hornworm, Manduca sexta.
After injection into male and female fifth-instar larvae of Manduca sexta, [14C]cholesterol was converted to C21 steroids, [14C]pregn-5-ene-3 beta,20-diols. These metabolites were isolated from 8-day-old pupae and were identified by TLC, HPLC, and GC-MS as the C-20 isomers of pregnene-3 beta,20-diol. They also were isolated from male and female meconium fluid (of 16-day-old pupae) following injection of [14C]cholesterol into 14-day-old pupae.
Lozano
R
R
Insect and Nematode Hormone Laboratory, ARS, USDA, Beltsville, MD 20705.
Thompson
M J
MJ
Svoboda
J A
JA
Lusby
W R
WR
Wilzer
K R
KR
D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007536:Isomerism; D007814:Larva / Q000378:metabolism; D007915:Lepidoptera / Q000378:metabolism*; D008297:Male; D011284:Pregnenolone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D011679:Pupa / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D002250:Carbon Radioisotopes; D011284:Pregnenolone; C009033:5-pregnene-3,20-diol; D002784:Cholesterol