Proteoglycans of the articular disc of the bovine temporomandibular joint. I. High molecular weight chondroitin sulphate proteoglycan.
Proteoglycans were extracted from articular discs with 4 M guanidinium chloride and separated by ion-exchange and gel-filtration chromatography into high molecular weight (Mr greater than 1,000,000) species containing chondroitin sulphate (predominantly chondroitin 6-sulphate) and low molecular weight iduronic acid-rich dermatan sulphate proteoglycan(s). The chondroitin sulphate proteoglycan contained 21% uronic acid and 17% protein. It gave rise on digestion with chondroitinase ABC to a protein core of apparent Mr 350,000 with an amino acid composition closely resembling that of the cartilage-type proteoglycans. Other characteristics shared with previously-described cartilage proteoglycans include the presence of keratan sulphate and the ability to aggregate with hyaluronic acid.
Nakano
T
T
Department of Oral Biology, University of Alberta, Edmonton, Canada.
Scott
P G
PG
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002358:Cartilage, Articular / Q000032:analysis*; D002417:Cattle; D002808:Chondroitin Lyases / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004588:Electrophoresis, Cellulose Acetate; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D011509:Proteoglycans / Q000302:isolation & purification*; D013704:Temporomandibular Joint / Q000032:analysis*; D014574:Uronic Acids / Q000032:analysis
D000596:Amino Acids; D011508:Chondroitin Sulfate Proteoglycans; D010446:Peptide Fragments; D011506:Proteins; D011509:Proteoglycans; D014574:Uronic Acids; D002808:Chondroitin Lyases