A fluorescence sandwich ELISA for detecting soluble and cell-associated human interleukin-2.
A fluorescence sandwich ELISA was developed for the quantitation of human interleukin-2 (IL-2) using a combination of two anti-human IL-2 antibodies, L61 and biotinylated anti-IL-2 rabbit IgG. The minimum level of IL-2 detectable by this ELISA was 0.3 pg/ml, indicating that this method is about 100 times more sensitive than the usual colorimetric human IL-2 ELISA. The ELISA specifically detected human IL-2 in cell culture supernatants and serum, and also in extracts from mitogen-stimulated peripheral blood mononuclear cells. All of the IL-2 species detected in the supernatants, sera and cell lysates were shown to have a molecular weight of 15,000, as estimated by size exclusion high performance liquid chromatography.
Kitamura
K
K
AIDS Research Center, National Institute of Health, Tokyo, Japan.
Matsuda
K
K
Ide
M
M
Tokunaga
T
T
Honda
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D013045:Species Specificity
D007376:Interleukin-2