title: The isolation of spermatozoa from sexual assault swabs using proteinase K.
abstract: The most common examination in sexual offences is the identification of spermatozoa in aqueous extracts from swabs. Using Proteinase K with sodium dodecyl sulphate, it is possible to digest all the cellular material apart from the spermatozoa, resulting in a quick and effective method of their isolation. Spermatozoa were detected in 18 extracts using this treatment when the normal method had failed to reveal all but one of them.
authors: R L
authors: RL
authors: Chapman
authors: N M
authors: NM
authors: Brown
authors: S M
authors: SM
authors: Keating
mesh_terms: D019286:Endopeptidase K; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D012697:Serine Endopeptidases / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate; D013094:Spermatozoa; D014621:Vagina / Q000378:metabolism*
chemical_list: D012967:Sodium Dodecyl Sulfate; D012697:Serine Endopeptidases; D019286:Endopeptidase K