title: Trichomonas vaginalis and Tritrichomonas foetus secrete neuraminidase into the culture medium.
abstract: Supernatants taken from axenic cultures of Trichomonas vaginalis and Tritrichomonas foetus contain a neuraminidase activity, the detection of which is augmented when the trichomonad culture media are supplemented with 30% supernatant of confluent epithelial cultures. The enzyme was active against human erythrocytes, which became highly reactive to peanut agglutinin lectin. The specificity of the enzyme was checked by using a substrate specific to neuraminidase: 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuramic acid.
authors: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
authors: F
authors: F
authors: Costa e Silva Filho
authors: E M
authors: EM
authors: Breier-Saraiva
authors: M X
authors: MX
authors: Tosta
authors: W
authors: W
authors: de Souza
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D004586:Electrophoresis; D004912:Erythrocytes / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D009439:Neuraminidase / Q000378:metabolism*; D019887:Peanut Agglutinin; D013379:Substrate Specificity; D014244:Trichomonas / Q000201:enzymology*; D014246:Trichomonas vaginalis / Q000201:enzymology*
chemical_list: D003470:Culture Media; D037102:Lectins; D019887:Peanut Agglutinin; D009439:Neuraminidase