A competition enzyme-linked immunosorbent assay (ELISA) for the measurement of pancreatic GP-2 glycoprotein.
A competition enzyme-linked immunosorbent assay has been developed for the quantitative detection of soluble and membrane-bound GP-2, a glycoprotein which is confined to the exocrine pancreas. Zymogen granule membranes fixed to microtiter plates with poly-L-lysine were used as the source of antigen. Detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS] was added to the assay in order to reveal all the antigens, more particularly in membranous samples. Presence of detergent at concentrations as high as 0.5% did not interfere with any particular steps of the ELISA. This competition ELISA can detect 10 ng of GP-2 and will be useful for measuring soluble as well as membrane GP-2 in order to elucidate its role in the secretory process of the pancreas as well as in certain pathologies such as cystic fibrosis.
Leblond
F A
FA
Centre de Recherche sur les Mécanismes de Sécrétion, Faculty of Science, University of Sherbrooke, Quebec, Canada.
Talbot
B G
BG
Lauzon
I
I
LeBel
D
D
D000818:Animals; D001667:Binding, Competitive; D002021:Buffers; D002793:Cholic Acids; D003550:Cystic Fibrosis / Q000209:etiology; D003594:Cytoplasmic Granules / Q000032:analysis; D003902:Detergents; D004721:Endoplasmic Reticulum / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D007797:Laminin / Q000302:isolation & purification* / Q000502:physiology; D008562:Membrane Glycoproteins / Q000302:isolation & purification; D010179:Pancreas / Q000032:analysis* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
D002021:Buffers; D002793:Cholic Acids; D003902:Detergents; D007797:Laminin; D008562:Membrane Glycoproteins; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate