Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy.
A spectral analysis of whole EDTA blood was undertaken by using attenuated total reflection and Fourier-transform infrared spectroscopy. The concentration of blood glucose was measured by an enzymatic method using glucose dehydrogenase and ranged between 40 and 290 mg/dL with an average concentration of 90.4 mg/dL. Multivariate calibration with the partial least-squares (PLS) algorithm was performed on spectral data between 1500 and 750 cm-1 showing a varying background from different unidentified interfering compounds. Cross validation was carried out for optimizing the PLS model. PRESS was 19.8 mg/dL, which was calculated on the basis of 127 standards, whereas the estimated standard deviation for the calibration fit was computed to be 11.9 mg/dL. Infrared spectroscopy can be used for monitoring glucose levels within the normal physiological range in a complex matrix like whole blood as an alternative to electrochemical sensors.
Heise
H M
HM
Marbach
R
R
Janatsch
G
G
Kruse-Jarres
J D
JD
D001786:Blood Glucose / Q000032:analysis*; D005583:Fourier Analysis; D006801:Humans; D015999:Multivariate Analysis; D013055:Spectrophotometry, Infrared / Q000379:methods / Q000706:statistics & numerical data*
D001786:Blood Glucose