Toxicity of IL-2 and Corynebacterium parvum following direct intracranial injection.
The toxicity of IL-2 and/or C. Parvum when injected directly into the mouse brain was examined by survival and histopathology using different numbers of injections, different doses of IL-2, different solvents for the IL-2, and different doses and routes of administration of C. Parvum. Two injections was found significantly to increase mortality (19%) over a single injection (4%). Mortality from two injections of 30,000 U (23%) or 60,000 U (20%) was higher than from two injections of 15,000 U (12%). The mortality from two injections with normal saline as solvent was much higher (29%) than from two injections with sterile water (19%) or D5W (9%). Two injections of IL-2 given simultaneously with C. Parvum showed a much higher mortality (26%) than other doses and routes of C. Parvum administration. Mice dying acutely (6-24 days) of toxicity showed an extensive mononuclear infiltrate at the site of injection. The brains of surviving mice (sacrificed at 30 days) showed a mild residual mononuclear cell infiltrate with the exception of mice which had received IL-2 and C. Parvum simultaneously. Brains from this latter group had an extensive residual mononuclear cell infiltrate.
Sutton
R C
RC
Section of Neurosurgery, Palo Alto Veterans Administration Medical Center, CA.
Kennedy
J
J
Duncan
J
J
Conley
F K
FK
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000633:toxicity*; D001921:Brain / Q000473:pathology; D005260:Female; D007276:Injections, Intraventricular; D007376:Interleukin-2 / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011425:Propionibacterium acnes / Q000276:immunology*
D001428:Bacterial Vaccines; D007376:Interleukin-2