Decline in growth and antibody production of established hybridomas and transfectomas with addition of interleukin 6.
Several sources of the lymphokine interleukin (IL) 6 (human recombinant IL 6, supernatant from P388D1, a murine macrophage cell line and murine thymocyte-conditioned media) were examined for enhancement of growth, immunoglobulin (Ig) secretion and seeding density requirements in murine hybridoma and transfectoma cell lines established in the absence of exogenously added IL 6. None of these preparations increased growth or Ig secretion in the six cell lines studied. In fact, inhibition of proliferation was seen with two of the three IL 6 preparations. Human recombinant IL 6 showed no effect on minimal seeding density, but murine thymocyte-conditioned media gave a tenfold decrease in minimal seeding density requirements in one of two hybridomas and one of two transfectomas studied. All preparations contained active IL 6, as measured by proliferation of the IL 6-dependent cell line T1165.D10. These data show that IL 6 alone does not increase growth or Ig secretion for these established cell lines. This finding is compared with previous reports describing increased growth in primary cultures of murine hybridomas and human myelomas with IL 6.
Karavodin
L M
LM
Hybritech Incorporated, San Diego, CA 92126.
Glasebrook
A L
AL
Phelps
J L
JL
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D006825:Hybridomas / Q000166:cytology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D066298:In Vitro Techniques; D015850:Interleukin-6; D007378:Interleukins / Q000494:pharmacology*; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection
D007074:Immunoglobulin G; D015850:Interleukin-6; D007378:Interleukins; D011994:Recombinant Proteins