Growth rate of human fibroblasts is repressed by the culture within reconstituted collagen matrix but not by the culture on the matrix.
Effect of reconstituted type I collagen gel on in vitro growth of fibroblasts was examined. A prolonged lag period was observed by culture either on the surface of three-dimensional collagen gel ("on-gel") or within the gels ("in-gel") as compared with the culture on plastic dishes. The rate of cell proliferation in logarithmic phase growth was repressed by the culture "in-gel" but not by the culture "on-gel". The differential growth rates between "in-gel" culture and "on-gel" culture should be ascribed to difference in distributions of interacting sites between cell and collagen fibrils. The repression of cell growth was more marked in a contracting collagen gel which contains higher density of collagen fibrils. The cell density in contracted gel per unit cross-section area was found to be much lower than that of confluent monolayer culture. These results suggest that the repression of cell growth by collagen fibrils in the three-dimensional gels is not due to direct cell-cell contact, but due to the distribution and number of contact sites between a cell and collagen fibrils.
Nishiyama
T
T
Shiseido Basic Research Laboratories, Yokohama, Japan.
Tsunenaga
M
M
Nakayama
Y
Y
Adachi
E
E
Hayashi
T
T
D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology* / Q000648:ultrastructure; D003470:Culture Media / Q000378:metabolism; D004058:Diffusion; D004815:Epidermal Growth Factor / Q000378:metabolism; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology*; D005782:Gels; D006801:Humans; D011392:Proline / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D003470:Culture Media; D005782:Gels; D004815:Epidermal Growth Factor; D003094:Collagen; D011392:Proline; D013936:Thymidine