An immunological study on chromogranin A and B in human endocrine and nervous tissues.
Antisera were raised against synthetic peptides derived from the primary amino acid sequence of human chromogranin B. These antisera recognized in one- and two-dimensional immunoblotting a component previously designated as chromogranin B. In human chromaffin granules, the major endogenous processing product of chromogranin B is formed by proteolytic cleavage of the protein near the C-terminus. Immunohistochemical localizations were obtained with antisera against human chromogranins A and B and against a synthetic peptide corresponding to the B sequence. In human tissues, chromogranin B is co-stored with chromogranin A in the adrenal medulla, the anterior pituitary, parafollicular cells of the thyroid, in some cells of the endocrine pancreas and in some enterochromaffin cells, whereas only chromogranin A is found in the parathyroid gland and enterochromaffin cells of the gastric corpus mucosa. In the nervous system, no immunostaining was observed for chromogranin A and only a weak one for chromogranin B in some cells of the spinal cord. However, the Purkinje cells of the cerebellum were strongly positive for chromogranin B.
Schmid
K W
KW
Department of Pathology, University of Innsbruck, Austria.
Weiler
R
R
Xu
R W
RW
Hogue-Angeletti
R
R
Fischer-Colbrie
R
R
Winkler
H
H
D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis*; D004702:Endocrine Glands / Q000032:analysis*; D006801:Humans; D007106:Immune Sera; D015151:Immunoblotting; D007150:Immunohistochemistry; D009417:Nerve Tissue / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*
C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D007106:Immune Sera; D009419:Nerve Tissue Proteins