title: Localization of the glucose phosphate isomerase gene to the p12----q21 segment of chromosome 6 in pig by in situ hybridization.
abstract: The glucose phosphate isomerase (GPI) locus is closely linked to the halothane sensitivity locus in pig. The chromosomal localization of GPI in pig was confirmed to 6p12----q21 by using in situ hybridization. Of the total grains, 25 percent were located on chromosome 6, with about 65 percent clustered in the cent----q21 segment, suggesting the presence of the GPI gene on the very proximal part of the q arm. The efficiency of hybridization was found to be affected by ultraviolet irradiation of metaphases for RBA-banding before hybridization. The irradiated metaphases had higher background grains than the non-irradiated metaphases.
authors: B P
authors: BP
authors: Chowdhary
authors: I
authors: I
authors: Harbitz
authors: A
authors: A
authors: Mäkinen
authors: W
authors: W
authors: Davies
authors: I
authors: I
authors: Gustavsson
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D013552:Swine / Q000235:genetics*
chemical_list: D005956:Glucose-6-Phosphate Isomerase