Some features of DNA fragments associated in vivo with the nuclear lamina.
Ehrlich Ascites Tumour cells were irradiated with UV-light to crosslink DNA to proteins in vivo. The DNA fragments associated with the nuclear lamina were purified and characterized. The results of the Cot analysis and the hybridization experiments suggest that the DNA fragments attached to the nuclear lamina although containing the entire complexity of genomic DNA are enriched in some highly repeated sequences.
Christova
R
R
Institute of Molecular Biology Bulgarian Academy of Sciences, Sofia.
Yaneva
J
J
Galcheva-Gargova
Z
Z
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism / Q000528:radiation effects; D009363:Neoplasm Proteins / Q000378:metabolism / Q000528:radiation effects; D009687:Nuclear Proteins / Q000378:metabolism / Q000528:radiation effects; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D004247:DNA