title: Ganglioside composition in experimental tumors with different growth properties.
abstract: The composition of gangliosides was studied in four fibrosarcomas (FL, FLA, FLB, FLC) induced in the Lewis rat by Ferridextran, and in two clones of a spontaneous Lewis rat mammary sarcoma (C-1-SAM LEW and C-2-SAM LEW) and two supertransformed clones (S-174 and S-271) derived from these clones, using the B77 virus. The malignancy of the Lewis tumors was tested in terms of their ability to outgrow the RT-5 barrier in LEW.1x rats and expressed as the loss-rate of LEW.1x rats. As for the Ferridextran-induced tumors, the FL was the only one to have been rejected in nearly 100% of the LEW.1x recipients. Following presensitization with FL the other tumors were also rejected, though not at a rate of 100%. The rat loss-rate was: FL--0%, FLC--23.5%, FLB--36.5%, and FLA--82.6%. As malignancy increased, the composition of gangliosides showed signs of progressive simplification indicating a step-by-step repression of ganglioside biosynthesis involving first the disialoganglioside and subsequently also the monosialoganglioside pathways. Some less distinct decrease (but significant at the 5% level of probability) of gangliosides of the disialoganglioside pathway and an increase of the simplest ganglioside (GM3) were observed between the C-1 clone of SAM LEW and its S-174 supertransformant. However, the changes, especially in the C-2 clone and its supertransformant, were not such as would suggest a marked defect in the biosynthesis of gangliosides.
authors: 1st Institute of Pathology, Charles University, Medical Faculty, Prague, Czechoslovakia.
authors: F
authors: F
authors: Smíd
authors: V
authors: V
authors: Kren
authors: V
authors: V
authors: Bradová
authors: P
authors: P
authors: Veselý
authors: D
authors: D
authors: Krenová
mesh_terms: D000818:Animals; D002455:Cell Division; D002472:Cell Transformation, Viral; D005354:Fibrosarcoma / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D005732:Gangliosides / Q000032:analysis* / Q000096:biosynthesis; D007505:Iron-Dextran Complex; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew / Q000235:genetics; D012509:Sarcoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D005732:Gangliosides; D007505:Iron-Dextran Complex