Thin-layer chromatography/bioautography method for detection of monensin in poultry tissues.
Monensin is an effective anticoccidial agent widely used in the poultry industry. Because of concerns over its toxicity, a sensitive, reliable, fast, and simple method for residue detection in poultry tissues is desirable from both a diagnostic and a regulatory view. Many methods of detection are excluded due to this ionophore's complex chemical and biological nature. A common method used for its detection is thin-layer chromatography/bioautography (TLC/B), although this is usually only semiquantitative. A new TLC/B method for monensin detection in poultry tissues was developed and is reported here. Recovery from cardiac muscle, skeletal muscle, and liver and kidney tissues is in the range of 93-97%. A detection limit of 250 micrograms/kg with 99% reliability was achieved with this method. Lower limits (to 10 micrograms/kg) were detectable, but with lower reliability (60%). Quantitative analysis is not possible on samples from fatty tissue.
Vanderkop
P A
PA
Toxicology Research Group, University of Saskatchewan, Saskatoon, Canada.
MacNeil
J D
JD
D000818:Animals; D001681:Biological Assay; D002645:Chickens; D002855:Chromatography, Thin Layer; D004350:Drug Residues / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008985:Monensin / Q000032:analysis*
D007202:Indicators and Reagents; D008985:Monensin