title: Expression of isoferritins in peripheral blood lymphocytes: effect of phytohaemagglutinin and iron.
abstract: This study shows that incubation of lymphocytes in phytohaemagglutinin medium together with iron supplementation leads to an increase of ferritin synthesis concomitant with increased intracellular ferritin concentration especially of heart-type ferritin. Increasing levels of iron do not increase heart-type ferritin concentration further; in contrast, spleen-type ferritin is gradually increased with increasing iron levels. The preferential increase in spleen-type ferritin on growth in iron-enriched medium may be explained by two distinct roles in the metabolism of these two isoferritins. Heart-type isoferritin appears to be involved in more active iron metabolism in lymphocytes whilst the spleen-type isoferritin may function as an intracellular depot of excess iron that is not immediately utilised for metabolic activity.
authors: Thalassaemia Centre, Faculty of Graduate Studies, Mahidol University, Bangkok, Thailand.
authors: K
authors: K
authors: Pattanapanyasat
mesh_terms: D002852:Chromatography, Ion Exchange; D005293:Ferritins / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology*
chemical_list: D010835:Phytohemagglutinins; D005293:Ferritins; D007501:Iron; D007770:L-Lactate Dehydrogenase