Immunohistochemical localization of interleukin 1 in human growth cartilage.
Although it has been reported that interleukin 1 (IL-1) stimulate chondrocytes to produce collagenase and proteoglycanase in vitro, IL-1 producing cells and the function of IL-1 have not been demonstrated in osteocartilaginous tissue in vivo. Immunohistochemical studies of human cartilaginous epiphysis and growth cartilage demonstrated that IL-1 was detected in: (1) chondrocytes surrounding cartilage canal, (2) hypertrophic chondrocytes in cartilaginous epiphysis, (3) chondrocytes at the hypertrophic and calcified zones in the growth cartilage of actively growing bone. In contrast, few hypertrophic chondrocytes showed positive reactions to IL-1 in growth plates nearing physiologic closure. Furthermore, IL-1 was detected in chondrocytes cultured from human growth cartilage. These results show that IL-1 is produced by matured chondrocytes of human growth cartilage in vivo. Chondrocyte-derived IL-1 might play a key role in the hypertrophy of chondrocytes, in the vascularization of cartilage and in the formation of bone.
Yamashita
F
F
Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, Japan.
Sakakida
K
K
Kusuzaki
K
K
Takeshita
H
H
Kuzuhara
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D002454:Cell Differentiation; D002455:Cell Division; D002648:Child; D005260:Female; D006132:Growth Plate / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D007375:Interleukin-1 / Q000032:analysis* / Q000502:physiology; D008297:Male; D008875:Middle Aged
D007375:Interleukin-1