An improved method of direct chromosome preparation from chorionic villus and high resolution banding technique.
The trophoblast was dissociated from the underlying mesenchymal layer either with acetic acid after short-term prefixation or with mechanical power after fixation twice. The colcemid treatment time was shorted to 16 min and trypsin solution of low pH (6.2) was used for banding. By these steps, the quality of chromosome banding was greatly improved and complete standard chromosome diagnoses were made in 24 of 24 cases. With the modified technique, high resolution banding chromosomes were consistently obtained after short-term incubation.
Xiao
S
S
Department of Biology, Harbin Medical University, China.
Liu
Q Z
QZ
Wang
R B
RB
Zhang
M Y
MY
Li
P
P
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D015193:Chorionic Villi Sampling / Q000379:methods*; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes / Q000187:drug effects; D003703:Demecolcine; D005260:Female; D006801:Humans; D011247:Pregnancy; D013048:Specimen Handling / Q000379:methods*
D000085:Acetates; D019342:Acetic Acid; D003703:Demecolcine