Turnover of fatty acyl-CoA oxidase in the liver of rats fed on a partially hydrogenated marine oil.
The change in turnover of fatty acyl-CoA oxidase (FAO), the rate-limiting enzyme of the peroxisomal beta-oxidation system, was investigated in rats fed a 30% (w/w) partially hydrogenated marine oil (PHMO) diet. The FAO activity increased five-fold after two weeks of PHMO feeding, and decreased after withdrawal of the diet. Based on in vivo experiments using L-[4,5-3H]leucine and an immunoprecipitation technique, the increase in the activity of FAO could be accounted for by a 1.6-fold higher rate of FAO synthesis and a 3.4-fold slower rate of FAO degradation as compared to controls. In the same PHMO-fed rats, the rates of synthesis and degradation of carnitine palmitoyltransferase were 1.8-fold higher and 2.0-fold slower, respectively, as compared to controls. The results indicate that the observed increase in the activity of the enzymes of peroxisomal beta-oxidation is mainly due to a reduced rate of FAO degradation in the liver of rats fed the PHMO diet.
Horie
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Department of Clinical Biochemistry, Tokyo College of Pharmacy, Japan.
Suga
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T
D042962:Acyl-CoA Oxidase; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D006207:Half-Life; D008099:Liver / Q000201:enzymology*; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D004042:Dietary Fats, Unsaturated; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; D002334:Carnitine O-Palmitoyltransferase