Human cerebrospinal fluid acetylcholinesterase and butyrylcholinesterase. Evidence for identity between the serum and cerebrospinal fluid butyrylcholinesterase.
Human cerebrospinal fluid contained both acetylcholinesterase (EC 3.1.1.7) and butyrylcholinesterase (EC 3.1.1.8) and they were estimated in the presence of selective inhibitors. Butyrylcholinesterase of human cerebrospinal fluid was similar to human serum butyrylcholinesterase in its electrophoretic mobility, glycoprotein nature and tyramine activation of the aryl acylamidase (EC 3.5.1.13) activity exhibited by butyrylcholinesterase. Moreover antibody raised against human serum purified butyrylcholinesterase could completely immunoprecipitate butyrylcholinesterase from human cerebrospinal fluid without affecting acetylcholinesterase. It is suggested that a useful method for the precise determination of acetylcholinesterase in human cerebrospinal fluid would be removal of butyrylcholinesterase by immunoprecipitation using antibody raised against human serum butyrylcholinesterase.
Rao
R V
RV
Department of Neurological Sciences, Christian Medical College and Hospital, Vellore, India.
Gnanamuthu
C
C
Balasubramanian
A S
AS
D000110:Acetylcholinesterase / Q000134:cerebrospinal fluid*; D000328:Adult; D000368:Aged; D000581:Amidohydrolases / Q000037:antagonists & inhibitors / Q000097:blood; D000906:Antibodies / Q000276:immunology; D002091:Butyrylcholinesterase / Q000097:blood / Q000134:cerebrospinal fluid* / Q000276:immunology; D002800:Cholinesterase Inhibitors; D002802:Cholinesterases / Q000134:cerebrospinal fluid*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D006801:Humans; D008875:Middle Aged; D009422:Nervous System Diseases / Q000201:enzymology; D011233:Precipitin Tests; D011342:Procainamide; D012685:Sepharose; D012701:Serotonin / Q000494:pharmacology; D014439:Tyramine / Q000494:pharmacology
D000906:Antibodies; D002800:Cholinesterase Inhibitors; D012701:Serotonin; D012685:Sepharose; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases; D000581:Amidohydrolases; C019732:aryl acylamidase; D011342:Procainamide; D014439:Tyramine