Incidence of anti-DNA idiotype-positive cells in human peripheral blood.
Human anti-DNA idiotype (Id)-bearing cells in peripheral blood were sought using mouse monoclonal anti-Id antibodies to human monoclonal anti-DNA antibodies. Pretreatment with acid pH or pronase P or preincubation in human serum-free medium markedly decreased the number of anti-Id-reactive cells. Pronase P-treated cells were able to bind to anti-Id once more after incubation for 18 hr at 37 degrees C, indicating the resynthesis of internal idiotypic determinants on the cells. Antiidiotype-reactive cells retained the same idiotypes in their cytoplasma. The cells expressing anti-DNA idiotypes, termed 0-81 and NE-1, were detected in the circulation of most patients with active lupus nephritis but not in those of inactive systemic lupus erythematosus (SLE) and healthy subjects. The idiotype-positive cells occurred in up to 5-10% of B cells from some patients with SLE in the active stage but became undetectable in remission. A limited number of anti-DNA Id-positive cells responsible for anti-DNA production might be preferentially expanded during acute episodes of the disease in some patients with SLE.
Takai
O
O
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Sasaki
T
T
Muryoi
T
T
Harata
N
N
Hashimoto
H
H
Yoshinaga
K
K
D000974:Antibodies, Antinuclear / Q000032:analysis*; D000921:Antibody-Producing Cells / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D004247:DNA / Q000276:immunology*; D006801:Humans; D007130:Immunoglobulin Idiotypes; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*
D000974:Antibodies, Antinuclear; D007130:Immunoglobulin Idiotypes; D004247:DNA