title: The amino acid sequence of Erythrina corallodendron lectin and its homology with other legume lectins.
abstract: The primary sequence of Erythrina corallodendron lectin was deduced from analysis of the peptides derived from the lectin by digestion with trypsin, chymotrypsin, Staphylococcus aureus V8 protease, elastase and lysylendopeptidase-C, and of fragments generated by cleavage of the lectin with dilute formic acid in 6 M guanidine hydrochloride. Purification of the individual peptides was achieved by gel filtration, followed by reverse phase HPLC. The glycosylation site (Asn17-Leu18-Thr19) was deduced from analysis of the glycopeptide isolated from a pronase digest of the lectin before and after deglycosylation of the glycopeptide with endoglycosidase F. Comparison of the sequence of 244 residues thus obtained with those of 9 other legume lectins revealed extensive homologies, including 39 invariant positions and 60 partial identities. These data provide further evidence for the conservation of the lectin gene in leguminous plants.
authors: Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
authors: R
authors: R
authors: Adar
authors: M
authors: M
authors: Richardson
authors: H
authors: H
authors: Lis
authors: N
authors: N
authors: Sharon
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004895:Erythrina; D007887:Fabaceae; D006020:Glycopeptides / Q000302:isolation & purification; D006031:Glycosylation; D037102:Lectins / Q000302:isolation & purification; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010946:Plants, Medicinal; D012689:Sequence Homology, Nucleic Acid
chemical_list: D006020:Glycopeptides; D037102:Lectins; D037121:Plant Lectins; C045629:erythrina lectin