title: Differentiation of intestinal epithelial cell line (IEC-18) by an acid extract of rat small intestine.
abstract: A factor which may induce differentiation of intestinal epithelial cell lines in vitro was found in an acid extract of adult rat small intestine. The addition of a partially purified acetic acid extract of rat small intestine to IEC-18 cell culture dishes increased sucrase activity within 48 h. Thymidine incorporation markedly decreased within 24 h. Significant development of microvilli-like structures was observed on the acid extract-treated IEC-18 cells, compared with controls. This activity of rat acid extract was heat-stable and the apparent molecular weight of the factor was 400-800. These findings suggested that the factor may be related to the epithelial differentiation of rat small intestinal crypt cells.
authors: Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
authors: T
authors: T
authors: Shintani
authors: T
authors: T
authors: Fushiki
authors: S
authors: S
authors: Fukuoka
authors: H
authors: H
authors: Takahashi-Iwanaga
authors: E
authors: E
authors: Sugimoto
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004261:DNA Replication / Q000187:drug effects; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D007421:Intestine, Small / Q000502:physiology*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D009130:Muscle, Smooth / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D014020:Tissue Extracts