Quick-freeze, deep-etch electron microscopy.
This chapter briefly introduces the quick-freeze replica studies carried out mainly by Japanese scientists. Focus is on this technique's contribution to revealing three-dimensional organization of the cytoskeleton, and membrane with associated structures.
Hirokawa
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D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure; D005615:Freezing; D051379:Mice; D008854:Microscopy, Electron