title: Acute effects of 1-methyl-1,4-phenylpyridinium ion (MPP+) on purine metabolism in rat striatum studied in vivo using the microdialysis technique.
abstract: In order to obtain further insight into the interactions between the purinergic and dopaminergic pathways in the striatum, we studied both metabolisms simultaneously, using a microdialysis technique in 1-methyl-1,4-phenylpyridinium ion (MPP+) unilaterally-denervated conscious rats. In these rats the contralateral side was used as control. The perfusates were collected every 20-25 min using 4 mm dialysis probes, implanted in each striatum, and assayed for dopamine and purine metabolites. After MPP+ administration, all adenosine metabolites - with the exception of uric acid - and dopamine levels were significantly increased in the extracellular medium. However, the time-course change in dopamine level did not correlate with the adenosine and inosine time-courses, suggesting a different mechanism of liberation in response to MPP+ administration.
authors: Dep. de Ciencies Fisiológiques, Humanes i de la Nutrició, Fac. de Medicina, Universitat de Barcelona, Spain.
authors: M
authors: M
authors: Ballarin
authors: J
authors: J
authors: Reiriz
authors: S
authors: S
authors: Ambrosio
authors: M
authors: M
authors: Camps
authors: R
authors: R
authors: Blesa
authors: N
authors: N
authors: Mahy
mesh_terms: D015655:1-Methyl-4-phenylpyridinium; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D011687:Purines / Q000378:metabolism*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D011687:Purines; D011726:Pyridinium Compounds; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine