Primary structure of a gene encoding rat T-kininogen.
A gene encoding the acute-phase reactant, T-kininogen, has been isolated from a rat genomic library. The nucleotide sequence of all exons as well as of a large portion of the introns was determined. The gene, T-KG, is approximately 24 kb in length and contains 11 exons. The promoter appears to be rather unusual in that there are no consensus CCAAT or TATAA boxes or SP1-binding sites. An A-rich region present upstream of the transcription start point may function as a TATAA-equivalent sequence. In addition, several potential glucocorticoid regulatory elements and a sequence similar to the AP-1 binding site are present in the 5' region of the gene. The intron/exon boundaries in this T-KG gene are identical to those seen in the human K-kininogen gene. This is consistent with the inclusion of T-KG in the cystatin supergene family of cysteine protease inhibitors.
Anderson
K P
KP
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, OH 45267.
Croyle
M L
ML
Lingrel
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D001330:Electronic Data Processing; D005091:Exons; D007704:Kininogens / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping
D007704:Kininogens; D004247:DNA