[Immunopathological analysis of T and non-T cell involvement in corneal inflammation caused by HSV type-1 infection].
In herpetic keratitis, the role of T cell subsets is not clearly understood although the involvement of cell-mediated immunity has been reported. The purpose of this paper is to analyse T cell-mediated immunopathological conditions and the role of non-T cell lineage in herpetic stromal keratitis employing nude (nu/nu) and Balb/c mice. All mice were divided into three groups. Group 1: Herpes simplex virus type 1 (HSV-1)-inoculated nu/nu mice. Group 2: HSV-1-inoculated nu/nu mice with interleukin-2 (IL-2). Group 3: HSV-1-inoculated Balb/c mice. The conditions of the disease were compared in those three groups clinically and by using histological and immunopathological staining. Following antibodies were employed: anti-Asialo GMI (natural killer). anti-Thy 1 (pan-T). anti-L3T4 (helper/inducer), anti-Lyt 2 (suppressor/killer), anti-Mac 3 (macrophage), anti-Mice Immunoglobulins (monocyte. B.). Corneal lesions showed a reduced inflammatory tendency in nu/nu mice. Topical application of IL-2 in nu/nu mice induced moderate stromal inflammation compared with nu/nu mice. However, the severity of these was less than in Balb/c. Many natural killer cells (NK) were found in nu/nu mice but few in Balb/c mice. These results confirm the involvement of T-cell mediated immunity in stromal inflammation and indicate that NK cell may have an important role in suppressed T-cell immunity in nu/nu mice but are not involved in stromal inflammation.
Kimura
T
T
Murata
Y
Y
Yamaguchi
T
T
Okumura
K
K
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D003319:Corneal Stroma / Q000276:immunology / Q000473:pathology; D005260:Female; D007111:Immunity, Cellular; D007150:Immunohistochemistry; D007378:Interleukins / Q000009:adverse effects; D007635:Keratitis, Dendritic / Q000276:immunology* / Q000473:pathology; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D007378:Interleukins