title: Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome.
abstract: Pulsed field gel electrophoresis and large DNA technology were used to construct a Not I restriction map of the entire genome of the fission yeast Schizosaccharomyces pombe. There are 14 detectable Not I sites in S. pombe 972h: 9 sites on chromosome I and 5 sites on chromosome II, while no Not I sites were found on chromosome III. The 17 fragments (including intact chromosome III) generated by Not I digestion were resolved by PFG electrophoresis. These fragments ranged in size from 4.5 kb to approximately 3.5 Mb. Various strategies were applied in determining, efficiently, the order of the fragments on the chromosomes. The genomic size measured by adding all the fragments together is about 14 Mb and the sizes of the three chromosomes are I, 5.7 Mb, II, 4.6 to 4.7 Mb, and III, 3.5 Mb. These are generally somewhat smaller than estimated previously.
authors: Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York 10032.
authors: J B
authors: JB
authors: Fan
authors: Y
authors: Y
authors: Chikashige
authors: C L
authors: CL
authors: Smith
authors: O
authors: O
authors: Niwa
authors: M
authors: M
authors: Yanagida
authors: C R
authors: CR
authors: Cantor
mesh_terms: D015139:Blotting, Southern; D002871:Chromosome Banding; D003001:Cloning, Molecular / Q000379:methods; D004271:DNA, Fungal / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific; D005800:Genes, Fungal; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping / Q000379:methods
chemical_list: D004271:DNA, Fungal; D015252:Deoxyribonucleases, Type II Site-Specific