Ornithine decarboxylase and trypanothione reductase genes in Leishmania braziliensis guyanensis.
Ornithine decarboxylase and trypanothione reductase are the key enzymes in polyamine and trypanothione metabolism in kinetoplastids. Using a heterologous Trypanosoma brucei brucei probe for ornithine decarboxylase and a mixed synthetic probe of 29 oligonucleotides for trypanothione reductase, we have detected the putative genes for these enzymes by Southern blot hybridization using genomic DNA of Leishmania braziliensis guyanensis MHOM/SR/80/CUMC 1. The trypanothione reductase probe was constructed both from the conserved codon usage of the redox active site for other flavin oxidoreductases over a wide evolutionary scale, and the preferred codon usage for other genes in species of Leishmania.
Keithly
J S
JS
Division of International Medicine, Cornell University Medical College, New York, New York 10021.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D006801:Humans; D007891:Leishmania / Q000235:genetics*; D007892:Leishmania braziliensis / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D009955:Ornithine Decarboxylase / Q000235:genetics*
D015345:Oligonucleotide Probes; D004247:DNA; D009247:NADH, NADPH Oxidoreductases; C049608:trypanothione reductase; D009955:Ornithine Decarboxylase