Suppression of c-myc mRNA expression by steroid hormones in HTLV-I-infected T-cell line, KH-2.
The effects of 1,25(OH)2D3 and dexamethasone on cellular proliferation and gene expression of the HTLV-I-infected T-cell line, KH-2, established from a patient with adult T-cell leukemia, endemic in the south-west Japanese islands and the Caribbean, were examined. KH-2 cells are integrated by HTLV-I proviral DNA and expressed mRNA for c-myc, IL-2 receptor alpha-chain (IL-2R alpha), and T-cell receptor beta-chain (TCR beta) while it did not express IL-2 mRNA. 1,25(OH)2D3 and dexamethasone did not suppress the mRNA levels of HTLV-I, IL-2R alpha or TCR beta but reduced the c-myc mRNA level. The reduction of c-myc mRNA level was marked in 1,25(OH)2D3-treated cells but relatively weak in dexamethasone-treated cells. This inhibitory effect of the steroid hormones correlated with the inhibition of KH-2 cell proliferation.
Koizumi
T
T
Department of Medicine, Kobe University School of Medicine, Japan.
Nakao
Y
Y
Kawanishi
M
M
Maeda
S
S
Sugiyama
T
T
Fujita
T
T
D015152:Blotting, Northern; D015139:Blotting, Southern; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D015342:DNA Probes; D003907:Dexamethasone / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000187:drug effects / Q000502:physiology; D009857:Oncogenes / Q000187:drug effects*; D012333:RNA, Messenger / Q000187:drug effects* / Q000235:genetics; D013489:Suppression, Genetic / Q000187:drug effects* / Q000235:genetics; D013601:T-Lymphocytes / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015342:DNA Probes; D007376:Interleukin-2; D012333:RNA, Messenger; D003907:Dexamethasone; D004247:DNA; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate