Changes in blood ketone body ratio with reference to graft viability after liver transplantation in rats.
Arterial blood ketone body ratio (acetoacetate/3-hydroxybutyrate; KBR), which reflects hepatic mitochondrial redox potential, was measured during a 2-week period after orthotopic liver transplantation in three groups of rats: group 1, the isogenic combination of LEW (RT1l) graft to LEW recipient as control; group 2, the allogenic combination of ACI (RT1a) graft to LEW recipient without immunosuppressive treatment: and group 3, the allogenic combination of ACI to LEW with immunosuppressive treatment using cyclosoporin (CyA). Isogenic recipients survived indefinitely. Allogenic recipients in group 2 had severe rejection with a mean survival of 10.3 +/- 0.54 days, while 77.8% of the allogenic recipients in group 3 survived more than 30 days. KBR of rats surviving more than 2 weeks in groups 1 and 3 gradually increased post-transplantation and was maintained at a high level. By contrast, though KBR in group 2 was restored at 3 days, it gradually fell and remained at a significantly low level (P less than 0.001). It is suggested that KBR provides an accurate indicator for evaluating metabolic viability of the critically deteriorating liver graft accompanied by sever rejection.
Asonuma
K
K
Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Tanaka
K
K
Uemoto
S
S
Okamura
R
R
Matsuoka
S
S
Kitakado
Y
Y
Utsunomiya
H
H
Katayama
T
T
Tanaka
M
M
Inomata
Y
Y
D000818:Animals; D003524:Cyclosporins / Q000494:pharmacology; D006084:Graft Rejection / Q000187:drug effects*; D007657:Ketone Bodies / Q000097:blood*; D008099:Liver / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D013997:Time Factors; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
D003524:Cyclosporins; D007657:Ketone Bodies