Crystallization and X-ray data collection on human growth hormone.
Single crystals of natural sequence human growth hormone have been grown from media containing ethanol, acetone or paraldehyde. Recombinant growth hormone in its native and desamidated form and pituitary hormone have been crystallized. A full native set of diffraction data extending to 3.5 A resolution has been obtained with synchrotron radiation for crystals of recombinant human growth hormone grown from ethanol. The identity of the material in these crystals has been established by anion-exchange chromatography.
Clarkson
J
J
Department of Chemistry, University of York, Heslington, England.
Korber
F
F
Christensen
T
T
Junker
F
F
Pedersen
J
J
Hansen
F B
FB
D002852:Chromatography, Ion Exchange; D003460:Crystallization; D013006:Growth Hormone; D006801:Humans; D014961:X-Ray Diffraction
D013006:Growth Hormone