[Chromogens in systems for immunoenzyme determination using horseradish peroxidase-labeled antibodies].
The author tested the suitability of various chromogens in ELISA systems with horseradish peroxidase. As a model he used assessment of human chorionic gonadotropin (HCG). The author evaluated the influence of substrate concentration on the resulting colour reaction and the influence of the presence of a detergent on the peroxidase activity. He investigated also the advantages of the used chromogens with regard to the accuracy and sensitivity of the estimation.
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D052160:Benzothiazoles; D006063:Chorionic Gonadotropin / Q000032:analysis; D002863:Chromogenic Compounds; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006735:Horseradish Peroxidase; D006801:Humans; D010655:Phenylenediamines; D013451:Sulfonic Acids
D052160:Benzothiazoles; D006063:Chorionic Gonadotropin; D002863:Chromogenic Compounds; D010655:Phenylenediamines; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C034193:1,2-diaminobenzene; D006735:Horseradish Peroxidase
Problematika chromogenů v systémech enzymoimunostanovení s pouzitím protilátky znacené krenovou peroxidázou.