Defining agonist peptides of human interleukin-1 beta.
The structure-function relationship in the human IL-1 beta protein has been analyzed by means of computer prediction, synthesis of peptides and use of monoclonal antibodies of predetermined specificity. A nine amino acid-long fragment (VQGEESNDK), corresponding to the human IL-1 beta sequence in position 163-171, was shown to possess only some of the IL-1 activities, i.e. those directed to the immune system, while being devoid of IL-1-like inflammatory effects. It is thus proposed that discrete domains within the IL-1 beta protein might be responsible for the different biological activities of the molecule and that the 163-171 fragment may represent one of the immunostimulatory sites of IL-1 beta.
Tagliabue
A
A
Sclavo Research Center, Siena, Italy.
Antoni
G
G
Boraschi
D
D
D000276:Adjuvants, Immunologic; D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D006801:Humans; D007107:Immune System / Q000502:physiology; D007375:Interleukin-1 / Q000494:pharmacology*; D053583:Interleukin-1beta; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D053583:Interleukin-1beta; D010446:Peptide Fragments; C052994:interleukin-1beta (163-171)