Effect of 5637-conditioned medium on peripheral blood granulocyte-macrophage progenitors in normal subjects and patients with the myelodysplastic syndrome.
There was no overall increase in PB-CFU-GM from normal subjects, or MDS patients, when exogenous CSA (5637-CM) was added to the culture medium. However, there was a sub-group of MDS patients (seven of 35) whose PB-CFU-GM numbers were significantly stimulated by 5637-CM. In addition, there were 11 (out of 48) MDS patients with undetectable PB-CFU-GM in assays without exogenous CSA but only two when 5637-CM was added (p less than 0.01). This sub-group is of particular interest as it is known that those without detectable PB-CFU-GM tend to have significantly shorter survival times than others. The mechanism of the functional abnormality is yet to be determined.
Tennant
G B
GB
Department of Haematology, University of Wales College of Medicine, Cardiff, U.K.
Jacobs
A
A
D000368:Aged; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D003470:Culture Media; D005260:Female; D006098:Granulocytes / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007958:Leukocyte Count; D008264:Macrophages / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000097:blood*; D011379:Prognosis
D003115:Colony-Stimulating Factors; D003470:Culture Media