Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts.
Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase. In mouse L cell fibroblasts reductase activity was restored with the concomitant metabolism of 25-hydroxycholesterol via side-chain hydroxylation and scission of the C20-C22 bond. Chinese hamster lung cells did not appear to derepress the reductase and these cells and Chinese hamster ovary cells did not metabolize 25-hydroxycholesterol to a significant extent. Only 5-10% of the oxysterol became esterified with a fatty acid in any of the cell lines when grown in the described culture conditions.
Taylor
F R
FR
Jackson Laboratory, Bar Harbor, ME 04609.
Kandutsch
A A
AA
D000818:Animals; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006888:Hydroxycholesterols / Q000378:metabolism*; D006900:Hydroxylation; D006903:Hydroxymethylglutaryl CoA Reductases / Q000032:analysis; D008168:Lung / Q000166:cytology; D051379:Mice; D010053:Ovary / Q000166:cytology; D011284:Pregnenolone / Q000096:biosynthesis*; D013997:Time Factors
D006888:Hydroxycholesterols; D011284:Pregnenolone; C007997:25-hydroxycholesterol; D006903:Hydroxymethylglutaryl CoA Reductases