title: Studies on the energy metabolism of opossum Didelphis virginiana erythrocytes--IV. Red cells have low adenosine deaminase activity and high levels of deoxyadenosine nucleotides.
abstract: Alkaline extracts of adult opossum red cells were used to determine triphosphates of adenosine, deoxyadenosine and guanosine by anion exchange HPLC. Mean (nm/g Hg) ATP content of erythrocytes was 3713 and that of dATP 1913 (n = 12). Sonicates of red cells deaminated adenosine (ADO) at a rate of 1.55 nm/mg Hg/h and deoxyadenosine (dADO) at 1.82 nm/mg Hg/h. dATP synthesis from provided dADO was one order of magnitude greater in opossum than in human erythrocytes at both low and high dADO and Pi concentrations.
authors: Department of Primary Care and Clinical Investigation, Fitzsimons Army Medical Center, Aurora, Colorado 80045.
authors: N C
authors: NC
authors: Bethlenfalvay
authors: E
authors: E
authors: Chadwick
authors: J E
authors: JE
authors: Lima
mesh_terms: D000241:Adenosine / Q000097:blood; D000243:Adenosine Deaminase / Q000097:blood*; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003838:Deoxyadenine Nucleotides / Q000097:blood*; D003839:Deoxyadenosines / Q000097:blood; D004734:Energy Metabolism; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000378:metabolism*; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009700:Nucleoside Deaminases / Q000097:blood*; D009893:Opossums / Q000097:blood*
chemical_list: D003838:Deoxyadenine Nucleotides; D003839:Deoxyadenosines; D006454:Hemoglobins; D000255:Adenosine Triphosphate; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D000241:Adenosine; C026600:2'-deoxyadenosine triphosphate