Lack of interspecies barriers in anti-Id stimulated antibody production against Echinococcus granulosus antigens.
Polyclonal human anti-hydatid antibodies were affinity purified from a hydatid patient serum and used to produce a rabbit anti-idiotypic serum. These anti-Id antibodies cross-reacted in ELISA with sera from 11 of 12 hydatid patients studied and with 13 infected or immunized mice sera. All mice primed and boosted with anti-Id produced anti-hydatid antibodies in the primary response and exhibited an increase in antibody titre after a booster injection. The same effect was observed with mice primed with antigen and boosted with anti-Id, although these mice exhibited higher antibody titres. A significant idiotype repertoire is shared by anti-hydatid antibodies produced by different individuals of the same or different species, and anti-Id raised against those antibodies behave as surrogate antigens producing a normal primary and secondary response in animals of different species from that used to isolate the Id.
Carol
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Cátedra de Inmunologia, Facultad de Quimica, Montevideo, Uruguay.
Hernandez
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Baz
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Nieto
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000947:Antigens, Helminth; D003429:Cross Reactions; D004443:Echinococcosis / Q000276:immunology / Q000517:prevention & control; D004446:Echinococcus / Q000276:immunology*; D005260:Female; D006801:Humans; D007114:Immunization; D007117:Immunization, Secondary; D007130:Immunoglobulin Idiotypes; D051379:Mice; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007130:Immunoglobulin Idiotypes