title: Platelets and polymorphonuclear leukocytes in experimental ocular inflammation in the rabbit eye.
abstract: Accumulation of 111indium-oxine (111In)-labelled platelets and the kinetics of 111In-labelled polymorphonuclear leukocytes (PMNLs) were studied in the anterior eye during neurogenic inflammation (induced by topical neutral formaldehyde) or after paracentesis in the rabbit, after formaldehyde irritation, 40 times more platelets were found in the aqueous humor 60 min later than in the control eyes and 400 times more after paracentesis. Platelets were found to be increased in the ciliary body, but not in the iris or choroid. Under light microscopy, some of the blood vessels in the ciliary processes were occluded and filled with red blood cells and platelets. The amount of PMNLs increased in the aqueous humor 15-20 h following formaldehyde irritation. After paracentesis, increased amounts of PMNLs were found in the iris and ciliary body 3-6 h later, while in the aqueous humor PMNLs were observed already 2 h. In the present study, increased amounts of 111In-labelled platelets and PMNLs were demonstrated in the anterior eye during experimental ocular inflammation. This method provides a useful tool for evaluating the accumulation of cells or the cell kinetics in ocular tissues during experimental inflammation.
authors: Department of Anatomy, University of Helsinki, Finland.
authors: K
authors: K
authors: Krootila
authors: M
authors: M
authors: Syrjälä
authors: J I
authors: JI
authors: Lehtosalo
authors: H
authors: H
authors: Uusitalo
mesh_terms: D000818:Animals; D000869:Anterior Eye Segment / Q000276:immunology*; D001082:Aqueous Humor / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D005557:Formaldehyde; D007205:Indium Radioisotopes; D007249:Inflammation / Q000276:immunology; D007700:Kinetics; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D011817:Rabbits
chemical_list: D007205:Indium Radioisotopes; D005557:Formaldehyde