Alterations in megakaryocyte and platelet compartments following in vivo IL-1 beta administration to normal mice.
IL-1 has been shown to stimulate the release of granulocyte-macrophage CSF, granulocyte-CSF, and macrophage-CSF from "accessory cell populations" in vitro, and it stimulates the appearance of colony-stimulating activity in the sera of mice in vivo. This cytokine has also been proposed to act on primitive hematopoietic progenitor cells to stimulate expression of receptors for the CSF. We sought to determine whether IL-1 beta could influence platelet and/or megakaryocytes and their progenitor cells following in vivo administration to normal mice. Our results demonstrated that, although administration of IL-1 beta clearly expands the pool of megakaryocyte-CFU and acetylcholinesterase-positive megakaryocytic cells (primarily in the spleen), it causes a transient and dose-dependent reduction of circulating platelets. The associated thrombocytopenia can be abolished by splenectomy before IL-1 beta administration, and is not temporally associated with the development of splenomegaly.
Williams
D E
DE
Department of Experimental Hematology, Immunex Corporation, Seattle, WA 98101.
Morrissey
P J
PJ
D000110:Acetylcholinesterase; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D002451:Cell Compartmentation / Q000187:drug effects; D005260:Female; D006412:Hematopoietic Stem Cells / Q000502:physiology; D007274:Injections, Intraperitoneal; D007375:Interleukin-1 / Q000008:administration & dosage*; D008533:Megakaryocytes / Q000145:classification / Q000201:enzymology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity / Q000187:drug effects; D010641:Phenotype; D010976:Platelet Count; D013156:Splenectomy
D007375:Interleukin-1; D000110:Acetylcholinesterase