Isolation of a female-specific, highly repeated Schistosoma mansoni DNA probe and its use in an assay of cercarial sex.
A 476 bp fragment of female-specific Schistosoma mansoni genomic DNA, clone W1, represents a degenerative repeat present in more than 500 copies per female genome, and may be part of the constitutive heterochromatin of the W chromosome. The cloning method described can be used as a general approach for isolating sex-specific, repeated DNA fragments. Using W1 as a probe, we have developed a rapid and accurate dot-blot assay for determining the sex of S. mansoni cercariae.
Webster
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P
Department of Pharmacology, Stanford University, CA 94305.
Mansour
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TE
Bieber
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D
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012550:Schistosoma mansoni / Q000235:genetics*; D012732:Sex Determination Analysis
D015342:DNA Probes; D004247:DNA