title: Primary culture of marrow core in collagen gels: modulation and transformation of endosteal cells. I. Morphologic observations.
abstract: It has been shown that collagen gels can be used as a culture matrix for the growth and proliferation of a variety of stromal and hemopoietic cellular elements. Since collagen is a physiologic matrix and allows the simultaneous growth and proliferation of a variety of cellular elements in three dimensional fashion, we used this method to culture 2 to 3 mm segments of trephined bone marrow. The gels were constantly bathed by RPMI 1640 medium containing fetal calf serum and antibiotic. Modulation, migration and transformation of endosteal cells were apparent under phase contrast microscopy and were confirmed in histologic sections. This study demonstrates for the first time that human marrow endosteal cells - the cells which line the bony trabeculae - are inducible and capable of modulation and transformation into stromal (fibroblast-like, macrophage-like, and fat-containing cells) and hemopoietic (round or spheroidal blast-like) cells. This report provides further evidence that endosteal cells can be considered the fixed (reserve) totipotential stem cells in the human bone marrow and are the equivalent of embryonal level undifferentiated mesenchymal cells which are capable of giving rise to the many different cell types that constitute the hemopoietic organ, i.e., bone marrow.
authors: Department of Medical Oncology, Roswell Park Memorial Institute, Buffalo, NY 14263.
authors: A
authors: A
authors: Islam
authors: R
authors: R
authors: Steiner
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D003094:Collagen; D003470:Culture Media; D046508:Culture Techniques; D005782:Gels; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans
chemical_list: D003470:Culture Media; D005782:Gels; D003094:Collagen