Specificity and timing of the Zn2+ requirement for DNA synthesis by 3T3 cells.
Addition of the chelator DTPA to synchronized cultures of 3T3 cells inhibited thymidine incorporation by up to 90% and only Zn2+ of the divalent cations tested was effective in reversing this effect. Fe2+ given alone had no effect on the inhibition by DTPA but when added to Zn2+ supplemented cultures increased thymidine incorporation from approximately 80-90 to over 100% of that in control cultures. Investigations indicated that the major requirement for Zn2+ was within the period from 8 h after stimulation of quiescent cells with serum until 3 h before the start of S phase. There was also an indication of a further requirement for Zn2+ at the G/S transition.
Chesters
J K
JK
Rowett Research Institute, Bucksburn, Aberdeen, United Kingdom.
Petrie
L
L
Vint
H
H
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002461:Cell Line, Transformed; D004247:DNA / Q000096:biosynthesis* / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism* / Q000502:physiology; D007399:Interphase; D051379:Mice; D004369:Pentetic Acid / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D015032:Zinc / Q000378:metabolism / Q000502:physiology*
D004369:Pentetic Acid; D004247:DNA; D015032:Zinc; D013936:Thymidine