title: Comparative studies on the adhesiveness of granulocytes of guinea pig and man.
abstract: The high affinity of granulocytes of guinea pig and man to glass surfaces is modified by serum. Native serum contains both an adherence-promoting activity, which is related to complement, and components which reduce the adhesiveness of granulocytes. These components are stable at 56 degrees C for 30 min and are tightly bound to the glass surface. beta-Lipoproteins are candidates for this adherence reducing ability of serum. Adherence promotion by native serum is mediated by coating the glass surface with C3b/C3bi. Human granulocytes from the peripheral blood adhered pig serum with C3b/C3bi to almost the same extent as in the presence of native serum, but on guinea pig granulocytes elicited in the peritoneal cavity, a cell surface metalloproteinase degraded the C3b/C3bi, thus reducing the adhesiveness of these cells. This proteinase was inhibited by MgEDTA, DTT, and 1,10-phenanthroline, whereby the high adhesiveness of granulocytes was restored to C3b/C3bi-coated glass.
authors: Sektion Biologie, Institut für Zoologie, Ernst-Moritz-Arndt-Universität, Greifswald, German Democratic Republic.
authors: D
authors: D
authors: Ehlers
authors: I
authors: I
authors: Sakowski
authors: W
authors: W
authors: Mohrig
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D002448:Cell Adhesion / Q000187:drug effects; D003179:Complement C3b / Q000378:metabolism / Q000502:physiology; D005898:Glass; D006098:Granulocytes / Q000502:physiology*; D006168:Guinea Pigs / Q000502:physiology*; D006801:Humans; D006834:Hydrazines / Q000494:pharmacology; D008666:Metalloendopeptidases / Q000378:metabolism; D013696:Temperature
chemical_list: D006834:Hydrazines; C029424:hydrazine; D003179:Complement C3b; D008666:Metalloendopeptidases