title: Isolation and characterization of a tumor-derived human pancreastatin-related protein.
abstract: A protein with pancreastatin-like immunoreactivity has been isolated and purified from liver metastasis of a patient with insulinoma. NH2-terminal sequence analysis in conjunction with the use of antibodies specific for the C-terminal structure of pancreastatin identified this protein as a 186-amino acid residue protein corresponding to human chromogranin A-116-301. Using a sensitive radioimmunoassay it was found that serum from the patient with insulinoma contains two peptide species; one comigrates with the 186-amino acid residue pancreastatin and the other the 48-residue pancreastatin.
authors: Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
authors: S
authors: S
authors: Funakoshi
authors: H
authors: H
authors: Tamamura
authors: M
authors: M
authors: Ohta
authors: K
authors: K
authors: Yoshizawa
authors: A
authors: A
authors: Funakoshi
authors: K
authors: K
authors: Miyasaka
authors: K
authors: K
authors: Tateishi
authors: K
authors: K
authors: Tatemoto
authors: I
authors: I
authors: Nakano
authors: H
authors: H
authors: Yajima
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D007340:Insulinoma / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D010187:Pancreatic Hormones / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification*; D011863:Radioimmunoassay
chemical_list: D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D010187:Pancreatic Hormones; D010446:Peptide Fragments; C050836:pancreastatin; C061408:chromogranin A (116-301) protein, human