title: [Determination of epoxide hydrolase activity by high performance liquid chromatography].
abstract: A new highly sensitive method of microsomal epoxide hydrolase activity has been worked out. The product of styrene oxide enzyme hydrolysis--phenylethyleneglycol (PEG) was extracted by n-butanol, and its aliquot was analysed by high-performance liquid-chromatography on Silica Gel using hexane-isopropanol-water (80:28:2) mobile phase. PEG was detected at 210 nm. The detection limit of PEG was 5 pmol per injection. The coefficient of variation of the method was 3.7%.
authors: L V
authors: LV
authors: Kravchenko
authors: V S
authors: VS
authors: Sobolev
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004851:Epoxide Hydrolases / Q000032:analysis*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004851:Epoxide Hydrolases
vernacular_title: Opredelenie aktivnosti époksidgidrolazy s ispol'zovaniem vysokoéffektivnoĭ zhidkostnoĭ khromatografii.