Vicia villosa lectin-positive neurones in human cerebral cortex. Loss in Alzheimer-type dementia.
Frontal and temporal sections of 4 normal human brains and 3 brains from patients with a diagnosis of Alzheimer-type dementia (ATD) were stained with Vicia villosa lectin conjugated to biotin to localize glycoproteins containing terminal N-acetylgalactosamine. These lectin binding sites were localized at the surface of a population of non-pyramidal interneurones. Examination of the co-existence of calbindin D28K or parvalbumin (PVA) with the lectin-binding material revealed that in the human cerebral cortex the lectin labelled only the PVA-immunoreactive neurones. Cell counts of the number of lectin-staining cells revealed that these cells are lost in ATD. The few remaining lectin-staining cells in ATD are shrunken and showed reduced expression of the lectin-staining material.
Kobayashi
K
K
MRC Group, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Emson
P C
PC
Mountjoy
C Q
CQ
D000116:Acetylgalactosamine / Q000378:metabolism*; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D002452:Cell Count; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D005688:Galactosamine / Q000031:analogs & derivatives*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D037102:Lectins; D037121:Plant Lectins
D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; C042242:Vicia lectins; D005688:Galactosamine; D000116:Acetylgalactosamine