[HLA class II typing of peripheral blood B lymphocytes separated using monoclonal antibodies].
The different methods proposed for preparation of B lymphocyte suspensions are not always simple, rapid and reliable. Presence of monocytes in the B cell suspension makes difficult HLA-DQ typing. For these reasons we propose a method using monoclonal antibodies (McAb) and complement to lyse the non B cells. Mononuclear cells, isolated from peripheral blood by Ficoll-Hypaque, are suspended in a mixture of anti-CD2, CD3, CD11b (OKM1 recognizing monocytes and granulocytes) McAb. This method of preparation of B cell suspension is rapid and provide better typing reactions than did the suspensions prepared by depletion of sheep red blood cell rosetting cells.
Lepage
V
V
Laboratoire d'Immunologie et d'Histocompatibilité, Hôpital Saint-Louis, Paris.
Raffoux
C
C
Douay
C
C
Mallet
C
C
Colombani
J
J
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001402:B-Lymphocytes; D002469:Cell Separation; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D006650:Histocompatibility Testing; D006801:Humans; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens
Groupage HLA classe II sur lymphocytes B du sang périphérique séparés à l'aide d'anticorps monoclonaux.