The effect of vitamin D3 on the production of cytotoxic factors by human peripheral blood mononuclear cells.
Vitamin D3 at a concentration of 300 nM was shown to significantly reduce the yields of cytotoxin produced by human peripheral blood mononuclear cells. Reduction of cytotoxin titers occurred when induction was performed with either concanavalin A, interleukin 2, or ionomycin. Typing of this cytotoxin was accomplished using antisera that were prepared by immunization of rabbits with either recombinant tumor necrosis factor or lymphotoxin. Both antisera were demonstrated to neutralize the cytotoxic activity produced by the peripheral blood mononuclear cells in the presence or absence of vitamin D3. These latter experiments suggested that the cytotoxin in question might be lymphotoxin. Vitamin D3 was also shown to decrease cytotoxin production by a transformed B-cell line known to produce only lymphotoxin, supporting the concept that this vitamin could diminish yields of lymphotoxin at least under certain circumstances. However, purified adherent cells induced by Escherichia coli lipopolysaccharide produced a cytotoxin that was also inhibited by the presence of vitamin D3. Indomethacin failed to influence the effects of vitamin D3 on the production of the cytotoxin. These studies suggest that vitamin D3 may have a role in regulating the secretion of cytotoxic factors which may be important in the defense against neoplastic diseases.
Yousefi
S
S
Department of Medicine, California College of Medicine, University of California Irvine 92717.
Vu
D
D
Carandang
G
G
Yamamoto
R
R
Granger
G
G
Vaziri
N
N
Ocariz
J
J
Cesario
T
T
D002117:Calcitriol / Q000494:pharmacology*; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003603:Cytotoxins / Q000096:biosynthesis*; D004987:Ethers / Q000494:pharmacology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D015759:Ionomycin; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology
D003603:Cytotoxins; D004987:Ethers; D007376:Interleukin-2; D011994:Recombinant Proteins; D003208:Concanavalin A; D015759:Ionomycin; D002117:Calcitriol; D007213:Indomethacin