Calcitonin gene-related peptide increases in rat facial motoneurons after peripheral nerve transection.
The rat facial nerve was transected and the retrograde reaction studied in the facial nucleus using light and electron microscopic immunohistochemistry and radioimmunoassay for calcitonin gene-related peptide (CGRP). An initial increase of CGRP-immunoreactivity (IR) was noted at 15 h after axotomy, thereafter CGRP-IR continued to rise to maximal levels around day 6 after which it gradually decreased and reached normal levels again after 5-6 weeks. Immunoreactive CGRP was found to fill the perikarya of facial motoneurons extending into dendrites and axons. Possible functional implications of CGRP as a neuroregulatory molecule during nerve regeneration are discussed.
Streit
W J
WJ
Department of Neuromorphology, Max Planck Institute for Psychiatry, Martinsried, F.R.G.
Dumoulin
F L
FL
Raivich
G
G
Kreutzberg
G W
GW
D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D003714:Denervation; D005154:Facial Nerve / Q000378:metabolism* / Q000601:surgery / Q000648:ultrastructure; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000378:metabolism* / Q000648:ultrastructure; D009416:Nerve Regeneration; D009457:Neuroglia / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D009479:Neuropeptides; D015740:Calcitonin Gene-Related Peptide