Cephalosporin penetration into soft tissue of paralyzed limbs.
Poor penetration of antibiotics into paralyzed tissue may contribute to the difficulty of curing soft tissue infections in paralyzed limbs. A novel model of spinal cord hemisection was used to induce paralysis of one hind leg in mice. Five, 10, or 20 days after induction of paralysis, six groups of 10 mice were injected intravenously with a single dose or with four sequential doses of cefepime, a new broad-spectrum cephalosporin, and then sacrificed. High-performance liquid chromatography was used to compare cefepime levels in soft tissue homogenates of paralyzed and normal hind legs; no significant differences were found in any group. Factors other than antibiotic delivery may be responsible for difficulty in curing infections in paralyzed soft tissue.
Darouiche
R
R
Medical Service (Infectious Disease Section), Houston Veterans Administration Medical Center, Texas.
Musher
D
D
Hamill
R
R
Ou
C
C
Rognerud
C
C
D000818:Animals; D000077723:Cefepime; D002511:Cephalosporins / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D006454:Hemoglobins / Q000378:metabolism; D006614:Hindlimb / Q000378:metabolism*; D051379:Mice; D009132:Muscles / Q000378:metabolism* / Q000473:pathology; D009929:Organ Size; D010243:Paralysis / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D002511:Cephalosporins; D006454:Hemoglobins; D000077723:Cefepime