title: Histological evidence of natural killer cell aggregation against malignant melanoma induced by adoptive immunotherapy with lymphokine-activated killer cells.
abstract: Adoptive immunotherapy with lymphokine-activated killer (LAK) cells and systemic administration of recombinant Interleukin-2 (RIL-2) was carried out in a case of malignant melanoma with lung metastases. Histological specimens from the lung showed a metastatic melanoma heavily invaded by atypical lymphoid cells with convoluted nuclei of varying size. Immunohistochemistry revealed that these cells had the characteristic exclusively of natural killer cell (Leu-7+). Nodules of these cells mimicked the appearance of non-Hodgkin's lymphoma of pleomorphic type. Molecular cytogenetic analysis, however, showed the absence of rearranged bands for the T-cell receptor beta-chain gene, indicating the absence of T-cell clones. At autopsy, 1 month after the LAK therapy, the heavy invasion of convoluted cells had disappeared. These findings clearly indicate that the LAK cell plus RIL-2 therapy induced Leu-7+ lymphoid cells, phenotypically suggestive of natural killer cell aggregation in the tumours.
authors: Department of Dermatology, Osaka University Medical School, Japan.
authors: T
authors: T
authors: Yamamura
authors: Y
authors: Y
authors: Fujitani
authors: T
authors: T
authors: Kawauchi
authors: E
authors: E
authors: Wada
authors: Y
authors: Y
authors: Kobayashi
authors: K
authors: K
authors: Yoshikawa
authors: H
authors: H
authors: Ogawa
authors: H
authors: H
authors: Sugiyama
authors: M
authors: M
authors: Ohsawa
authors: K
authors: K
authors: Aozasa
mesh_terms: D002449:Cell Aggregation; D006801:Humans; D007116:Immunization, Passive; D007376:Interleukin-2; D007694:Killer Cells, Natural / Q000473:pathology*; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary*; D008222:Lymphokines; D008297:Male; D008545:Melanoma / Q000473:pathology / Q000556:secondary*; D008875:Middle Aged; D012878:Skin Neoplasms / Q000473:pathology*
chemical_list: D007376:Interleukin-2; D008222:Lymphokines