Modified liquid chromatographic method for determination of gentian violet in animal feed.
A modified liquid chromatographic method is described for the determination of Gentian Violet (GV) in animal feed. The reliable detection limit is 0.5 ng (reference standards), and 1 ppm GV was reliably determined in feed. The calibration curve was linear between 1 and 40 micrograms/mL. The method, developed in a study by the National Center for Toxicological Research, was modified to use methanol-water (9 + 1) instead of benzene-methanol as the eluting solution in the column cleanup. GV is extracted from feed with methanol-1N HCl (99 + 1), cleaned up on a Sephadex LH-20 column to remove any remaining interferences, separated on a Nova-Pak C18 column fitted with a precolumn filter, and determined at 588 nm. The identity of GV is confirmed by thin-layer chromatography (Rf = 0.47) by comparison with a reference standard. Average recoveries from 3 sets of 5 feed samples containing 2.5, 5.0, and 10.0 ppm GV were 115, 95, and 102%, respectively.
Martinez
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Food and Drug Administration, Denver Federal Center, CO 80225-0087.
Shimoda
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D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D005840:Gentian Violet / Q000032:analysis*; D007202:Indicators and Reagents; D012015:Reference Standards
D007202:Indicators and Reagents; D005840:Gentian Violet