A polymorphic monoclonal antibody, PLM10, that reacts with B-cell lines carrying HLA-DPw1, DPw5, and DP "Cp63".
A monoclonal antibody, PLM10, that reacted with B-cell lines of DPw1, DPw5, and DP "Cp63" phenotypes was produced. The antibody was IgM isotype and efficiently lysed B cells but not T cells from approximately 50% of normal Japanese, raising the possibility of serologic typing of the DP polymorphism by the conventional complement-dependent cytotoxicity test.
Maeda
H
H
Blood Transfusion Service, Saitama Medical Center, Saitama Medical School, Japan.
Hirata
R
R
Hitomi
Y
Y
Tohyama
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D015180:Electrophoresis, Gel, Two-Dimensional; D006682:HLA-DP Antigens / Q000032:analysis*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011110:Polymorphism, Genetic
D000911:Antibodies, Monoclonal; D006682:HLA-DP Antigens