Phorbol esters stimulate a phospholipase D-catalysed reaction with both ester- and ether-linked phospholipids in HeLa cells.
The phorbol ester, 12-tetradecanoylphorbol-13-acetate (TPA), stimulated the accumulation of labelled phosphatidylethanol (PEth) in HeLa cells prelabelled with [3H]hexadecanol and incubated in the presence of ethanol. PEth formation involves a phospholipase D-catalysed phosphatidyl transferase reaction. [3H]Hexadecanol labelled both ester- and ether-derivatives of HeLa cell phospholipids and TPA stimulated the conversion of both classes of lipid to PEth.
Hii
C S
CS
School of Biological Sciences, Flinders University, Bedford Park, South Australia.
Kokke
Y S
YS
Pruimboom
W
W
Murray
A W
AW
D004987:Ethers; D005233:Fatty Alcohols / Q000378:metabolism; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D013379:Substrate Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004987:Ethers; D005233:Fatty Alcohols; D010743:Phospholipids; C005031:cetyl alcohol; D010740:Phospholipases; D010739:Phospholipase D; D013755:Tetradecanoylphorbol Acetate