Targeting of a plasma cell line with a conjugate containing xanthine oxidase and the monoclonal antibody 62B1.
We report on the preparation of an antibody-conjugated enzyme consisting of xanthine oxidase, a free-radical-producing enzyme, linked to the 62B1 monoclonal antibody, which recognizes the last steps of differentiation of B cell lineage (plasma cell and hairy cells). The conjugate specifically kills target cells, retaining both enzymic and immunological properties, without any damage to normal myeloid clonogenic efficiency. The model is suitable for ex vivo bone marrow purging in multiple myeloma patients.
Tazzari
P L
PL
Istituto di Ematologia Seràgnoli, Universitá di Bologna, Italy.
Battelli
M G
MG
Abbondanza
A
A
Dinota
A
A
Rizzi
S
S
Gobbi
M
M
Stirpe
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000633:toxicity*; D001853:Bone Marrow / Q000276:immunology; D002374:Catalase / Q000633:toxicity; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000633:toxicity; D000922:Immunotoxins / Q000633:toxicity*; D007700:Kinetics; D051379:Mice; D010950:Plasma Cells / Q000276:immunology*; D013482:Superoxide Dismutase / Q000633:toxicity; D014969:Xanthine Oxidase / Q000633:toxicity*
D000911:Antibodies, Monoclonal; D007042:Hypoxanthines; D000922:Immunotoxins; D019271:Hypoxanthine; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase