A marsupial phosphoglycerate kinase (PGK) processed pseudogene.
A clone that cross-hybridized with a full-length human cDNA PGK probe was isolated from a hill kangaroo (Macropus robustus: Marsupialia) lambda EMBL4 EcoRI genomic library. The clone was sequenced and demonstrated to be a pseudogene, with two deletions (one of 3 bases, the other 24 bases long), one single base insertion, and a nonsense mutation with respect to the functional human X-linked gene. It is flanked by terminal repeats in the 5' and 3' noncoding regions, but it has no 3' poly(A) remnant. The 3' untranslated region has a 34-bp sequence, with 29 bp homologous to the human 3' untranslated region. The overall percentage homology with the mouse and human X-linked PGK indicates that this pseudogene is probably more closely related to eutherian X-linked PGK genes than to the autosomal form. The results also suggest that pseudogenes are of considerable antiquity (greater than 100 MYr) in the mammalian lineage.
van Daal
A
A
School of Biological Sciences, Macquarie University, New South Wales, Australia.
Cooper
D W
DW
Molloy
P L
PL
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes; D006801:Humans; D007614:Macropodidae / Q000235:genetics*; D008394:Marsupialia / Q000235:genetics*; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D011544:Pseudogenes; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D010735:Phosphoglycerate Kinase