Enzyme linked immunosorbent assay for measuring antithrombin III.
A competitive ELISA for the measurement of antithrombin III antigen was developed using a pure preparation of antithrombin. This two step immunoassay requires only one antibody binding step and avoids the binding of anti-antithrombin antibody to the solid phase. Plasma samples measured by this ELISA correlated well with measurements taken with immune electrophoresis measurements (r = 0.933); the ELISA was also shown to be both sensitive and precise.
Edgar
P
P
Department of Haematology, Addenbrooke's Hospital, Cambridge.
Jennings
I
I
Harper
P
P
D000990:Antithrombin III / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans
D000990:Antithrombin III