Characterization of an S-type lectin purified from porcine heart.
We have purified a carbohydrate-binding protein from porcine heart by affinity chromatography on asialofetuin-Sepharose and have characterized this protein with respect to its size, amino acid composition, partial amino acid sequence, and carbohydrate-binding specificity. Porcine heart lectin (PHL) has a subunit molecular mass of 14,700 and is immunologically cross-reactive with a polyclonal antibody raised against a lectin isolated from calf heart. The amino acid composition of PHL is similar to that of lectins that have been isolated from calf heart, bovine brain, and rat lung. Moreover, the primary sequences of four tryptic fragments (52 amino acids total) derived from PHL are closely related to sequences previously determined for 10 other vertebrate-derived lectins. The ability of PHL to agglutinate rabbit erythrocytes was inhibited only by oligosaccharides containing terminal beta-galactosyl residues. These data indicate that PHL is a vertebrate "S-type" lectin and provide further evidence that the structures and carbohydrate-binding specificities of these lectins are highly conserved across diverse vertebrate genera.
Merkle
R K
RK
Department of Biochemistry, School of Chemical Sciences, University of Georgia, Athens 30602.
Zhou
Q
Q
Schultz
T K
TK
Harper
W B
WB
Cummings
R D
RD
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002642:Chick Embryo; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004593:Electrophorus; D006801:Humans; D037102:Lectins / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000032:analysis*; D010446:Peptide Fragments / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014357:Trypsin
D002352:Carrier Proteins; D037102:Lectins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins; D014357:Trypsin