Ultrastructural localization of calcium in post-mortem bovine muscle: a cytochemical and X-ray microanalytical study.
Calcium localization was demonstrated in bovine longissimus muscle using the antimonate precipitation technique in combination with electron probe X-ray microanalysis. Samples were taken each hour during the first 24 h post-mortem, and then after a storage period of 8 and 15 days. For all sampling times analysed, heavy precipitates were seen in dense parts of nuclei and on N-lines of myofibrils. Up to 18-20 h post-mortem, deposits were observed in sarcoplasmic reticulum at the level of triads. In comparison with the earlier post-mortem samples, myoplasmic precipitates were strongly increased at 4 h post-mortem, and just before rigor onset, at 19 h where intermyofibrillar spaces were completely blackened and triads were no more visible. These localizations of precipitates were still observed up to 15 days post-mortem. At these storage times, myofibril disruptions were seen at the level of N-lines. Wavelength-dispersive and energy-dispersive spectrometric analyses indicated that significant amounts of calcium occurred in the dense precipitates observed.
Vignon
X
X
INRA-Station de Recherches sur la Viande, Ceyrat, France.
Beaulaton
J
J
Ouali
A
A
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002417:Cattle; D004577:Electron Probe Microanalysis; D006651:Histocytochemistry; D009132:Muscles / Q000032:analysis* / Q000648:ultrastructure; D013057:Spectrum Analysis; D013997:Time Factors
D002118:Calcium