Murine trophoblast can be killed by allospecific cytotoxic T lymphocytes generated in GIBCO Opti-MEM medium.
Previous work in this laboratory demonstrated that a population of cultured midterm murine trophoblast cells are not susceptible to lysis by allospecific cytotoxic T lymphocytes (CTL) generated by standard in vitro protocols. We now report that this trophoblast population is killed, in an MHC-dependent manner, by allospecific CTL generated in GIBCO Opti-MEM, a modified tissue culture medium designed to maintain cell growth and proliferation in the presence of low concentrations of fetal bovine serum (FBS).
Drake
B L
BL
Cecil and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas 75235.
Head
J R
JR
D000818:Animals; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology*
D003470:Culture Media