title: Measurement of lung water dynamics and lung aeration in the perinatal period by use of digital X-ray fluoroscopy.
abstract: A method has been developed for the measurement of lung water dynamics and regional aeration of the lung in anesthetized newborn lambs by use of X-ray fluoroscopy, video recording, and digital image processing. After cesarean section and before the first breath fetal lambs under halothane-oxygen were placed on an X-ray table and connected to a volume-cycled respirator. X-ray fluoroscopy commenced before the initiation of respiration, and the images were recorded on video tape. X-ray transmission through the thorax increased as the lung was aerated. The enhanced transmission was compared with the values obtained from a calibration water wedge from which an equivalent path length through water can be estimated. In testing this method, it was demonstrated that X-ray transmission was linearly related to the wet lung weight-to-body weight ratio and to the product of the wet-to-dry weight ratio multiplied by anatomic thickness of frozen lung blocks. Calibrated values were also linearly related to this product and to the actual measured height of the fluid and tissue in the fluid-filled lung.
authors: Department of Obstetrics and Gynaecology, University of Calgary, Alberta, Canada.
authors: J E
authors: JE
authors: Maloney
authors: Y
authors: Y
authors: Takahashi
authors: C
authors: C
authors: Kondo
authors: W M
authors: WM
authors: Schoel
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001834:Body Water / Q000502:physiology*; D005260:Female; D005471:Fluoroscopy; D066298:In Vitro Techniques; D008168:Lung / Q000000981:diagnostic imaging / Q000502:physiology*; D011247:Pregnancy; D011856:Radiographic Image Enhancement; D012119:Respiration; D012756:Sheep