Three-dimensional reconstruction of mammalian 40 S ribosomal subunit.
The small (40 S) subunit from rabbit reticulocyte ribosomes has been reconstructed from electron micrographs of a negatively stained single-particle specimen to a resolution of 3.85 nm. The reconstruction reveals a morphology consisting of a broad wedge-shaped head structure set atop a quasi-cylindrical body. Distinctive features recognized in two-dimensional projections, such as the beak, back lobes, and feet, can now be localized in three dimensions. By reference to a recent reconstruction of the monomeric 80 S ribosome we can identify the interface and exterior surfaces of the subunit, thus enabling more detailed functional interpretations.
Verschoor
A
A
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Zhang
N Y
NY
Wagenknecht
T
T
Obrig
T
T
Radermacher
M
M
Frank
J
J
D000818:Animals; D002477:Cells / Q000648:ultrastructure*; D005057:Eukaryotic Cells / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008954:Models, Biological; D011817:Rabbits; D012156:Reticulocytes / Q000648:ultrastructure; D012270:Ribosomes / Q000648:ultrastructure*