title: Mechanism for the generation of active smooth muscle inhibitory factor (IF) from bovine retractor penis muscle (BRP).
abstract: The active smooth muscle inhibitory factor (IF) is generated by acidifying the extracts from the bovine retractor penis muscle (BRP). The activated IF is very similar to endothelium-derived relaxing factor (EDRF). However, the mechanism for the generation of active IF is yet unknown. From reverse-phase high performance liquid chromatographic data, the activities of nitrite loss were closely related to the contents of both sulfhydryl (SH) groups and L-cysteine. Thus, under acidic conditions, RSH and nitrite in the extracts are considered to react as follows: RSH + HNO2----RSNO + H2O, RSNO----(1/2) RSSR + NO, NO + (1/2)O2----NO2, NO2 + (1/2)H2O----(1/2)HNO2 + (1/2)HNO3. In addition to NO, RSNO is also a labile but potent vasodilator. Thus, activated IF seems to consist of RSNO and NO, and thiol-containing small molecules (molecular weight of less than 1,000) from BRP are closely involved in the generation of active IF.
authors: Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
authors: Y
authors: Y
authors: Yui
authors: S
authors: S
authors: Ohkawa
authors: K
authors: K
authors: Ohnishi
authors: R
authors: R
authors: Hattori
authors: T
authors: T
authors: Aoyama
authors: M
authors: M
authors: Takahashi
authors: H
authors: H
authors: Morishita
authors: Y
authors: Y
authors: Terao
authors: C
authors: C
authors: Kawai
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000096:biosynthesis*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D003545:Cysteine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008297:Male; D009126:Muscle Relaxation; D009130:Muscle, Smooth / Q000378:metabolism*; D010413:Penis; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D001685:Biological Factors; D013438:Sulfhydryl Compounds; C033031:smooth muscle inhibitory factor; D003545:Cysteine