Transcriptional pattern of 21-hydroxylase gene (P-450C21) during embryonic development, before, and after birth in mice as determined by in situ hybridization.
21-Hydroxylase is a member of the P-450 superfamily of genes involved in the biosynthesis of cortisol and aldosterone in the adrenal cortex. Congenital adrenal hyperplasia, a well-characterized disease, originates from a lack of this enzyme. We present in this report an in situ hybridization study aimed at detecting 21-hydroxylase activity during murine development, from mid gestation to adulthood. Our results demonstrate that even during the embryonic period the adrenal cortex is the only major site of transcription of this enzyme, which is detectable beginning at embryonic day 14. In addition, a peculiar topographical pattern of transcriptional activity, characteristic of the stage of differentiation of the gland, could be drawn. Using a computer-assisted method, we were able to quantitate the relative transcription level at each stage of development. A steady increase in the level of transcription was demonstrated throughout embryonic life to birth, with a drop during the prepubertal period and a final rise at adult age. The possible physiological significance of our findings is discussed.
Raschellà
G
G
Division of Biophysics, CRE Enea Casaccia, Rome, Italy.
Smets
G
G
Claeys
A
A
Verdood
P
P
Romeo
A
A
Hooghe-Peters
E L
EL
D000302:Adrenal Cortex / Q000196:embryology / Q000378:metabolism* / Q000502:physiology; D000375:Aging / Q000502:physiology; D000818:Animals; D003198:Computer Simulation / Q000379:methods; D004247:DNA / Q000032:analysis; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology* / Q000254:growth & development; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012984:Software; D013255:Steroid 21-Hydroxylase / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D013250:Steroid Hydroxylases / Q000235:genetics*; D014158:Transcription, Genetic
D004247:DNA; D013250:Steroid Hydroxylases; D013255:Steroid 21-Hydroxylase