Diagnostic use of an analysis of urinary proteins by a practicable sodium dodecyl sulfate-electrophoresis method and rapid two-dimensional electrophoresis.
Two methods suitable for routine clinical analyses of urinary proteins are presented and compared. The first is a horizontal sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique, suitable for simultaneous analysis of 20 native urinary samples. This method uses polyacrylamide gradient gels, prepared with a laboratory-built gel casting device. The second method is a rapid two-dimensional electrophoresis procedure, combining cellulose acetate electrophoresis and sodium dodecyl sulfate-electrophoresis. The first step uses a routine system (Chemetron), the second separation step followed by staining with Coomassie Brilliant Blue R is performed on the PhastSystem. The resulting two-dimensional patterns reveal urinary proteins distributed according to the 5-zone pattern of native proteins (albumin, alpha-1, alpha-2, beta, gamma-globulin) as well as to the logarithm of their molecular weights. Examples of (routine) diagnoses with a special interest in the monitoring of kidney transplant patients are shown.
Lapin
A
A
Institut für klinische Chemie und Laboratoriumsdiagnostik, Universität Wien, Austria.
Gabl
F
F
Kopsa
H
H
D002482:Cellulose / Q000031:analogs & derivatives; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D010449:Peptide Mapping; D011507:Proteinuria / Q000175:diagnosis*; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate; C005062:acetylcellulose; D002482:Cellulose