Interleukin-1 accelerates autocrine growth of myeloma cells through interleukin-6 in human myeloma.
Recombinant interleukin 1 alpha (rIL-1 alpha) augmented proliferation of freshly isolated myeloma cells as well as B-cell stimulatory factor 2 (BSF-2)/interleukin-6 (IL-6). Recombinant IL-1 alpha-induced proliferation was partially inhibited by anti-IL-6 antibody. In the culture supernatants of rIL-1 alpha-stimulated myeloma cells, IL-6 activities, which were measured by using an IL-6-dependent murine hybridoma clone, MH60.BSF2, were increased, when compared with those in the culture supernatants of nonstimulated myeloma cells. Furthermore, IL-6 messenger RNA (mRNA) expression was also augmented in IL-1 alpha-stimulated myeloma cells. Therefore rIL-1 alpha stimulates myeloma cells to produce IL-6, which consequently augments proliferation of myeloma cells. Thus, IL-1 can accelerate autocrine growth of myeloma cells through IL-6.
Kawano
M
M
Department of Internal Medicine, Hiroshima University, Japan.
Tanaka
H
H
Ishikawa
H
H
Nobuyoshi
M
M
Iwato
K
K
Asaoku
H
H
Tanabe
O
O
Kuramoto
A
A
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D001667:Binding, Competitive; D001853:Bone Marrow / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D009101:Multiple Myeloma / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D007106:Immune Sera; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D011994:Recombinant Proteins