title: Osteocalcin as an index of osteoblast function during and after ovine pregnancy.
abstract: Fetal and neonatal calcium requirements impose heavy demands on maternal bone and mineral homeostasis. The functional response of maternal osteoblasts to this stress is poorly understood. Therefore, plasma osteocalcin (OC) levels were measured by homologous RIA in age-matched nonpregnant, pregnant, and postpartum ewes to evaluate osteoblast function. In pregnant ewes from day 35 of gestation to term, the plasma OC level was suppressed to 8.2 +/- 0.5 micrograms/liter (mean +/- SEM; n = 36) compared with age-matched nonpregnant ewes (18.3 +/- 1.1 micrograms/liter; n = 39; P less than 0.0005). Plasma OC rose to the nonpregnant value by day 20 postpartum and was elevated above this level for the following 40 days (e.g. 44.0 +/- 5.0 micrograms/liter at 48-53 days; P less than 0.0005). The timing of changes in plasma OC levels and weaning did not correlate. The validity of plasma OC measurement as a marker of osteoblast function was assessed by determining the OC plasma production and clearance rates using an [125I]ovine OC infusion method. The OC plasma production rates in matched controls (n = 6), pregnant (n = 9), and 48-53-day postpartum sheep (n = 7) were 1.5 +/- 0.2, 0.5 +/- 0.04 (P less than 0.001 vs. control), and 3.6 +/- 0.6 mg/day (P less than 0.005 vs. pregnant sheep), respectively. In one ewe studied longitudinally, the OC plasma production rate increased by 15 days after parturition and achieved a 10-fold elevation at 49 days postpartum. The OC plasma clearance rate (3.3 +/- 0.3 liters/h) was the same in control, pregnant, and postpartum ewes. It is concluded that 1) changes in plasma OC levels during and after ovine pregnancy reflected changes in OC production, 2) plasma OC measurements are likely to be a useful index of osteoblast function in pregnancy, and 3) osteoblast function appears to be depressed during ovine pregnancy and enhanced markedly in the interval 20-60 days postpartum. The relationship between osteoblast function, as indicated by OC production, and bone formation remains to be clarified.
authors: University of Melbourne, Department of Medicine (Royal Melbourne Hospital), Victoria, Australia.
authors: W
authors: W
authors: Farrugia
authors: C L
authors: CL
authors: Fortune
authors: J
authors: J
authors: Heath
authors: I W
authors: IW
authors: Caple
authors: J D
authors: JD
authors: Wark
mesh_terms: D000375:Aging; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001842:Bone and Bones / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000097:blood*; D005260:Female; D005865:Gestational Age; D010006:Osteoblasts / Q000502:physiology*; D015675:Osteocalcin; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D012016:Reference Values; D012756:Sheep
chemical_list: D015415:Biomarkers; D002135:Calcium-Binding Proteins; D015675:Osteocalcin