Hemoglobin induction by Ara-C in human erythroleukemic cells (K562) is cell-cycle dependent.
This study was undertaken to answer the question of whether chemical induction of hemoglobin in the human erythroleukemic line K562 is cell-cycle dependent. Cells were synchronized with respect to the cell cycle by manipulation of culture conditions. S phase was detected microscopically by immunofluorescent staining. Hemin and arabinofuranosylcytosine (Ara-C) were used to induce hemoglobin synthesis in K562 cells. The action of hemin causes induction without specificity in regard to cell cycle phase, while Ara-C is dependent upon cell cycle phase, with cells within late G1 and early S phases being sensitive to the effect of the agent. These studies also confirm the results that the action of hemin is reversible but the induction caused by Ara-C is irreversible. The synergistic effects of these two inducing agents resulted in a sharper rise in the percentage of induced cells.
Wanda
P E
PE
Department of Biological Sciences, Southern Illinois University, Edwardsville 62026-1651.
Walker
M M
MM
D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology*; D006418:Heme / Q000494:pharmacology; D006454:Hemoglobins / Q000096:biosynthesis*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism* / Q000473:pathology
D006454:Hemoglobins; D003561:Cytarabine; D006418:Heme; D006918:Hydroxyurea