Compaction-defective embryonal carcinoma cell variants.
Compaction of the morula is a prerequisite for subsequent differentiation of the mouse embryo. Analogous differentiation follows compaction in aggregates of several embryonal carcinoma cell lines. This report describes the isolation of two compaction-defective variants from the H6 embryonal carcinoma cell line. These were isolated directly as clonal compaction-defective aggregates in medium containing 1.3% methylcellulose. They were obtained following chemical mutagenesis, since spontaneous variants were not seen. Compaction-defective variants of the F9 ECC line or the ES-D3 embryonic stem cell line could not be obtained. One of the H6 compaction-defective variants appeared to be dominant when hybridized to its parental line, while the other appeared to be recessive.
Littlefield
J W
JW
Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002449:Cell Aggregation; D002454:Cell Differentiation; D054278:Embryonal Carcinoma Stem Cells; D006651:Histocytochemistry; D006822:Hybrid Cells; D008747:Methylcellulose / Q000494:pharmacology; D051379:Mice; D014411:Neoplastic Stem Cells / Q000473:pathology / Q000502:physiology*; D014407:Tumor Cells, Cultured
D008747:Methylcellulose; D002122:Calcium Chloride