Cell-free translation in lysates from Spodoptera frugiperda (Lepidoptera: Noctuidae) cells.
1. Conditions for in vitro translation of mRNA in cell-free extracts from cultured Spodoptera frugiperda cells were defined. 2. Incorporation of [35S]methionine into acid-precipitable material increased for approximately 1 hr, and was sensitive to the protein synthesis inhibitors pactamycin and cycloheximide. 3. Micrococcal nuclease-treated lysate, primed with purified rabbit globin mRNA, synthesized a major protein with the size of full length globin, indicating that the lysate supported correct initiation and elongation of polypeptides.
Swerdel
M R
MR
Department of Entomology, University of Minnesota, St Paul 55108.
Fallon
A M
AM
D000818:Animals; D002457:Cell Extracts; D002474:Cell-Free System; D007915:Lepidoptera / Q000502:physiology*; D014176:Protein Biosynthesis; D014779:Virus Replication
D002457:Cell Extracts