title: Lymphocyte subsets of the peripheral blood in Sjögren's syndrome and rheumatoid arthritis.
abstract: Lymphocyte subsets were determined in the peripheral blood from twenty-three patients with primary Sjögren's syndrome (SS) and sixteen patients with clinically active rheumatoid arthritis (RA) by two-color flowcytometry using various monoclonal antibodies. In both diseases, T-cells (CD3+), suppressor/cytotoxic cells (CD8+) and their cytotoxic subset (CD8+CD11-) were decreased, as compared with thirty-one healthy controls. B-cells (CD 21+ and CD 3-DR+) and activated T-cells (CD 3+DR+) were increased in SS patients. Helper T-cells (CD 4+Leu8-), suppressor-inducer T-cells (CD4+Leu8+), suppressor T-cells (CD8+Leu 15+) and three natural killer (NK) cell subsets determined by both CD16 and Leu7 antibodies did not differ between controls and SS or RA, although Leu7+NK cells were significantly increased in SS patients. In addition, we found that the treatment with low-dose prednisolone decreased B-cells and suppressor-inducer T cells, and increased suppressor T-cells, cytotoxic T-cells and Leu7+NK cells. The results indicate similar changes in the proportion of lymphocyte subsets and suggest immunologically activated and deficient conditions in both diseases. Immunomodulating effects of the treatment with low-dose prednisolone on some of the lymphocyte subsets in patients with these diseases were also supported by the study.
authors: Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa, Japan.
authors: Y
authors: Y
authors: Ichikawa
authors: H
authors: H
authors: Shimizu
authors: K
authors: K
authors: Takahashi
authors: M
authors: M
authors: Yoshida
authors: J
authors: J
authors: Moriuchi
authors: M
authors: M
authors: Takaya
authors: S
authors: S
authors: Arimori
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D001172:Arthritis, Rheumatoid / Q000097:blood*; D001402:B-Lymphocytes / Q000145:classification; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007694:Killer Cells, Natural / Q000145:classification; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification*; D008297:Male; D008875:Middle Aged; D012859:Sjogren's Syndrome / Q000097:blood*; D013223:Statistics as Topic; D013601:T-Lymphocytes / Q000145:classification; D013602:T-Lymphocytes, Cytotoxic
chemical_list: D000911:Antibodies, Monoclonal