title: Carcinoma of the anal canal and flow cytometric DNA analysis.
abstract: Using flow cytometric DNA analysis of paraffin embedded tissue, DNA histograms were successfully obtained from the anal cancers of 117 patients. DNA diploid patterns were given by 82 cancers (70%) and DNA non-diploid patterns by 35 cancers (30%): 15 DNA aneuploid, 20 DNA tetraploid. Well differentiated squamous cell cancers were mainly DNA diploid, while a larger proportion of poorly differentiated and small cell cancers were DNA non-diploid. The large majority of stage A cancers were DNA diploid. A greater proportion of tumours that had invaded through the anal sphincter or had lymph node metastases or distant spread were DNA non-diploid. Prognosis was slightly poorer for patients with DNA non-diploid cancers when compared to patients with DNA diploid tumours (P = 0.08) and significantly poorer for individuals with DNA aneuploid anal cancers (P = 0.037). However, in a multivariate analysis model, the DNA ploidy pattern of an anal cancer was not of independent prognostic significance alongside tumour histology and tumour stage.
authors: Division of Pathology, Mayo Clinic, Rochester, Minnesota.
authors: N A
authors: NA
authors: Scott
authors: R W
authors: RW
authors: Beart
authors: L H
authors: LH
authors: Weiland
authors: S S
authors: SS
authors: Cha
authors: M M
authors: MM
authors: Lieber
mesh_terms: D000368:Aged; D001005:Anus Neoplasms / Q000032:analysis* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000032:analysis; D002295:Carcinoma, Transitional Cell / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011003:Ploidies; D011379:Prognosis
chemical_list: D004273:DNA, Neoplasm