Biochemical studies of the iron cores and polypeptide shells of haemosiderin isolated from patients with primary or secondary haemochromatosis.
Haemosiderin isolated from different iron-loading syndromes, primary haemochromatosis (PHC) and secondary haemochromatosis (SHC) biochemically exhibited differences in both their iron core and peptide composition. The rate of release of iron from PHC haemosiderin to oxalate was 3-fold greater than that from SHC haemosiderin. The major peptides separated by SDS-PAGE showed a major band at Mr 20,000 for PHC haemosiderin and at Mr 15,000 for SHC haemosiderin.
Ward
R J
RJ
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London, U.K.
O'Connell
M J
MJ
Dickson
D P
DP
Reid
N M
NM
Wade
V J
VJ
Mann
S
S
Bomford
A
A
Peters
T J
TJ
D003676:Deferoxamine; D005293:Ferritins / Q000302:isolation & purification; D006432:Hemochromatosis / Q000209:etiology / Q000378:metabolism*; D006485:Hemosiderin / Q000302:isolation & purification*; D006801:Humans; D007501:Iron / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008970:Molecular Weight; D013154:Spleen / Q000032:analysis*
D005293:Ferritins; D006485:Hemosiderin; D007501:Iron; D003676:Deferoxamine