Transforming growth factor-beta inhibits the generation of cytotoxic T cells in virus-infected mice.
The immunoregulatory effects of human recombinant transforming growth factor (rTGF) beta 1 and human recombinant glioblastoma-derived T cell suppressor factor (rG-TsF)/TGF beta 2 was investigated in mice infected with lymphocytic choriomeningitis virus (LCMV) or vaccinia virus. Starting on the day of infection, i.p. injections of 1 microgram/day or rTGF-beta 1 or rG-TsF/TGF-beta 2 suppressed the generation of virus specific CTL. The effect of TGF-beta on CTL (day 8) was less pronounced when TGF-beta treatment was delayed for 3 days after LCMV infection. rG-TsF/TGF-beta 2 also has an inhibiting effect on CTL-mediated disease in LCMV-infected mice: it prolonged the survival time of mice infected with LCMV and reduced the local swelling reaction after infection into the footpad. These results indicate that rTGF-beta 1 and rG-TsF/TGF-beta 2 influence T cell immune reactivity in vivo.
Fontana
A
A
Section of Clinical Immunology, University Hospital, Zürich, Switzerland.
Frei
K
K
Bodmer
S
S
Hofer
E
E
Schreier
M H
MH
Palladino
M A
MA
Zinkernagel
R M
RM
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004487:Edema / Q000276:immunology; D005260:Female; D006131:Growth Inhibitors / Q000494:pharmacology*; D006614:Hindlimb; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008216:Lymphocytic Choriomeningitis / Q000276:immunology* / Q000382:microbiology; D008217:Lymphocytic choriomeningitis virus / Q000254:growth & development; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D011994:Recombinant Proteins / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects*; D015291:Transforming Growth Factors / Q000494:pharmacology*
D006131:Growth Inhibitors; D011994:Recombinant Proteins; D015291:Transforming Growth Factors