The role of activation of neutrophils and microvascular pressure in acute pulmonary edema.
Activated polymorphonuclear neutrophils (PMN) can mediate vascular injury in the lung. This study compared activated aggregate PMN (emboli) to activated PMN that were previously adhered to the microvasculature (non-embolic) in the isolated perfused rat lung. Permeability and microvascular pressure (Pmv), components of PMN-induced edema, were examined by continuous measurement of wet weight, pulmonary arterial and left atrial pressures, and by intermittent determination of double occlusion pressure. PMN that were activated with phorbol myristate acetate and then perfused into the lung formed aggregates that lodged primarily in the precapillary bed, increasing arterial resistance. Although these PMN had minimal direct contact with the capillary endothelium, edema rapidly developed and Pmv was progressively elevated. If PMN were allowed to adhere in the capillary bed, a minimal and nonprogressive increase in Pmv and lung weight occurred. When these adherent PMN were then activated, there was a progressive rise in both Pmv and lung weight. The free radical scavenger catalase prevented this edema formation but not the rise in pressure. In control lungs with matched elevation of Pmv, edema did not develop. In another group of lungs with activation of pre-adherent PMN in which Pmv was maintained at control levels, edema formation was greatly delayed. These data show that: (1) the activated PMN free radical products alone caused permeability injury in the lung because neither contact of the PMN with the capillary endothelium nor embolization was necessary, and (2) increased Pmv does not cause edema but greatly increases the rate of edema formation when the endothelium is injured.
Patterson
C E
CE
Department of Physiology/Biophysics, Indiana University School of Medicine, Indianapolis 46223.
Barnard
J W
JW
Lafuze
J E
JE
Hull
M T
MT
Baldwin
S J
SJ
Rhoades
R A
RA
D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D002196:Capillaries / Q000473:pathology / Q000503:physiopathology*; D002200:Capillary Resistance / Q000502:physiology; D002374:Catalase / Q000494:pharmacology; D002448:Cell Adhesion; D002449:Cell Aggregation; D004730:Endothelium, Vascular / Q000473:pathology; D008168:Lung / Q000098:blood supply* / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D009929:Organ Size; D011654:Pulmonary Edema / Q000473:pathology / Q000503:physiopathology*; D011655:Pulmonary Embolism / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002374:Catalase; D013755:Tetradecanoylphorbol Acetate