Mitochondrial aspartate aminotransferase by immunoprecipitation method in patients with acute myocardial infarction.
The semiautomated kinetic procedure for determining cytoplasmic aspartate aminotransferase (c-AST) and mitochondrial aspartate aminotransferase (m-AST) activities was studied by the use of the immunoprecipitation method with anti-c-AST antibody in serum samples. The measured activity for m-AST remained constant after the addition of c-AST up to 1,000 IU/l throughout the 60-min incubation period. The measurements of m-AST activity were reproducible, selective and complete as determined by a purified m-AST. The precision of this method was as good as that of the manual method (CV 2.04%). The present method and the manual method gave approximately equal results for m-AST (r = 0.987). The effects of activations on m- and c-AST activity were compared by the addition of pyridoxal 5'-phosphate to sera of various diseases. A higher activation ratio by pyridoxal 5'-phosphate was observed on both aspartate aminotransferase activities in the serum of patients with ischemic heart diseases than in the serum of patients with liver diseases.
Okabe
H
H
Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
Sugiuchi
H
H
Yamane
N
N
Watanabe
N
N
Inoue
M
M
Morino
Y
Y
Hayasaki
K
K
Teranishi
H
H
Inayama
S
S
D000328:Adult; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D009203:Myocardial Infarction / Q000201:enzymology*; D011233:Precipitin Tests; D012016:Reference Values
D001219:Aspartate Aminotransferases