[The killing action of a Chinese herbal mixture on human tumor cells in vitro and its effect on cell-cycle].
Previous studies had shown that injection of Chinese herbal mixture (ICHM) had antitumor and anti-metastatic actions on various transplantable animal tumors. In the present study, the authors observed the obvious killing actions of ICHM on human gastric cancer cell line BGC-823 and breast cancer cell line MCF-7 in vitro. The IC50 were 0.02 g crude drug/ml and 0.018 g crude drug/ml respectively. The study of the effect of ICHM on the cell-cycle of BGC-823 cell showed that ICHM decreased the number of cells in S phase and increased the number of cells in G1 phase significantly. The results indicated that the BGC-823 cells was blocked on S phase by ICHM. Meanwhile, ICHM reduced 3H-TdR incorporation into DNA, the cpm value was much lower than that of the control.
Zhang
Y J
YJ
Wang
N Q
NQ
D000972:Antineoplastic Agents, Phytogenic; D001943:Breast Neoplasms / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; D004365:Drugs, Chinese Herbal