A cytotoxic serine proteinase isolated from mouse submandibular gland.
We have isolated a novel cytotoxic factor from the submandibular glands of male BALB/c mice by Sephadex G-50 gel filtration chromatography and reverse-phase HPLC. The cytotoxic factor is a serine proteinase, which belongs to the mouse glandular kallikrein (mGK) family, with an Mr of approximately 27,000. The purified serine proteinase showed cytotoxic activity against mouse thymocytes in a dose-dependent manner, and a serine proteinase inhibitor, diisopropyl fluorophosphate, blocked its cytotoxic activity.
Shimamura
T
T
Department of Bacteriology and Immunology, Showa University School of Medicine, Tokyo, Japan.
Nagumo
N
N
Ikigai
H
H
Murakami
K
K
Okubo
S
S
Toda
M
M
Ohnishi
R
R
Tomita
M
M
D000818:Animals; D002478:Cells, Cultured; D007531:Isoflurophate / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000633:toxicity; D015842:Serine Proteinase Inhibitors; D013363:Submandibular Gland / Q000201:enzymology*; D013601:T-Lymphocytes / Q000187:drug effects
D015842:Serine Proteinase Inhibitors; D007531:Isoflurophate; D010664:Phenylmethylsulfonyl Fluoride; D012697:Serine Endopeptidases