A possible autocrine role for interleukin-6 in two lymphoma cell lines.
Interleukin-6 (IL-6) is a growth factor with diverse biologic activity. Originally described as a T-cell product that enhances immunoglobulin (Ig) secretion in antigen-stimulated B cells, it also affects the growth of T cells, plasmacytomas, hybridomas, and hematopoietic stem cells. We report the expression and secretion of IL-6 by two lymphoma cell lines, OCI-LY3 and OCI-LY12. Addition of recombinant IL-6 stimulated their growth, whereas addition of polyclonal anti-recombinant IL-6 (anti-rIL-6) had a marked inhibitory effect on proliferation. These results suggest an autocrine role for IL-6 in the growth of these lymphoma cells in culture.
Yee
C
C
Department of Medicine and Medical Biophysics, Ontario Cancer Institute, Toronto, Canada.
Biondi
A
A
Wang
X H
XH
Iscove
N N
NN
de Sousa
J
J
Aarden
L A
LA
Wong
G G
GG
Clark
S C
SC
Messner
H A
HA
Minden
M D
MD
D015152:Blotting, Northern; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004273:DNA, Neoplasm / Q000302:isolation & purification; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D008223:Lymphoma / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000302:isolation & purification; D012260:Ribonucleases; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D004273:DNA, Neoplasm; D006133:Growth Substances; D007106:Immune Sera; D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger; D012260:Ribonucleases