Expression of fibrinogen receptors in platelets of migraine patients--correlation with platelet GPIIb content and plasma cholesterol.
Binding of fibrinogen to platelets washed from blood of migraine patients (n = 30) and control donors (n = 24) was compared. In addition, contents of platelet glycoprotein IIb and platelet fibrinogen were determined in both groups by radioimmunoassay. The receptor capacity for fibrinogen in platelets activated by ADP was significantly higher (p less than 0.01) in migraine patients (52,505 +/- 4,925) than in controls (33,881 +/- 3,965). The mean contents of GPIIb (3.51 +/- 0.34 micrograms/10(8) platelets) and fibrinogen (37.26 +/- 4.05 micrograms/10(8) platelets) in migraine platelets were also markedly increased (p less than 0.01 and p less than 0.001, respectively) when compared to controls (2.21 +/- 0.18 micrograms of GPIIb and 18.75 +/- 2.29 micrograms of fibrinogen per 10(8) platelets, respectively). There was a high correlation between the number of fibrinogen receptors exposed by ADP and the total amount of platelet GPIIb both in migraine patients (R = 0.69, p less than 0.01) and controls (R = 0.62 p less than 0.01), as well as plasma cholesterol in the case of migraine patients (R = 0.82, p less than 0.001).
Walkowiak
B
B
Department of Biophysics, Medical School, Lódź, Poland.
Kozubski
W
W
Pawlowska
Z
Z
Prusinski
A
A
Cierniewski
C S
CS
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D008881:Migraine Disorders / Q000097:blood*; D010980:Platelet Membrane Glycoproteins / Q000032:analysis* / Q000378:metabolism; D011863:Radioimmunoassay
D010980:Platelet Membrane Glycoproteins; D005340:Fibrinogen; D002784:Cholesterol