Autoradiographic localization of binding sites for epidermal growth factor-urogastrone (EGF-URO) in coronary arteries.
Previously published data have indicated that epidermal growth factor-urogastrone (EGF-URO) can regulate blood flow in vivo and can modulate vascular smooth muscle contractility in vitro. However, the exact location of the receptors responsible for this vascular action of EGF-URO has not been previously determined. In this report, employing in vitro autoradiography with 125I-labeled EGF-URO, we have documented the presence of specific EGF-URO binding sites in the tunica media of bovine and porcine coronary arteries. Our observations support the indirect evidence pointing to presence of EGF-URO receptors on the smooth muscle elements of arterial tissue. Our data also support further the hypothesis that EGF-URO and related growth factors (like TGF-alpha or PDGF) may play a role in normal and pathophysiological vascular function.
Gan
B S
BS
Department of Pharmacology and Therapeutics, University of Calgary, Faculty of Medicine, Alberta, Canada.
Hollenberg
M D
MD
D000818:Animals; D001345:Autoradiography; D002417:Cattle; D003331:Coronary Vessels / Q000187:drug effects / Q000378:metabolism; D066246:ErbB Receptors / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D013552:Swine
D007457:Iodine Radioisotopes; D066246:ErbB Receptors