Analysis by partitioning in aqueous two-phase systems of the loss of transferrin-binding capacity during maturation of rat reticulocytes.
A decrease in the number of binding sites for 125I-transferrin, without an apparent modification of the association constant, has been observed during the maturation of reticulocytes into erythrocytes. As an experimental model, different red cell populations from phenylhydrazinic anaemic rates (95% to 12% reticulocyte-rich) have been used. The fractionation by multiple partition in two-phase systems of these red cell populations has been applied here to show the relationship between number of transferrin receptors and rate of reticulocyte maturation.
Mendieta
J
J
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
Herráez
A
A
Sancho
P
P
Luque
J
J
D000740:Anemia / Q000097:blood; D000818:Animals; D001665:Binding Sites; D003377:Countercurrent Distribution; D004920:Erythropoiesis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011990:Receptors, Transferrin / Q000302:isolation & purification / Q000378:metabolism; D012156:Reticulocytes / Q000166:cytology / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism*
D011990:Receptors, Transferrin; D014168:Transferrin