title: Estimation of local cerebral protein synthesis rates with L-[1-11C]leucine and PET: methods, model, and results in animals and humans.
abstract: We have estimated the cerebral protein synthesis rates (CPSR) in a series of normal human volunteers and monkeys using L-[1-11C]leucine and positron emission tomography (PET) using a three-compartment model. The model structure, consisting of a tissue precursor, metabolite, and protein compartment, was validated with biochemical assay data obtained in rat studies. The CPSR values estimated in human hemispheres of about 0.5 nmol/min/g agree well with hemispheric estimates in monkeys. The sampling requirements (input function and scanning sequence) for accurate estimates of model parameters were investigated in a series of computer simulation studies.
authors: Department of Radiological Sciences, UCLA School of Medicine, Los Angeles, CA 90024.
authors: R A
authors: RA
authors: Hawkins
authors: S C
authors: SC
authors: Huang
authors: J R
authors: JR
authors: Barrio
authors: R E
authors: RE
authors: Keen
authors: D
authors: D
authors: Feng
authors: J C
authors: JC
authors: Mazziotta
authors: M E
authors: ME
authors: Phelps
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000097:blood; D004032:Diet; D006801:Humans; D007700:Kinetics; D007930:Leucine / Q000097:blood; D008253:Macaca mulatta; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051381:Rats; D014055:Tomography, Emission-Computed / Q000379:methods
chemical_list: D002250:Carbon Radioisotopes; D009419:Nerve Tissue Proteins; D007930:Leucine