Interleukin-1 induces a pertussis toxin-sensitive increase in diacylglycerol accumulation in mouse thymoma cells.
The mechanism of action of the cytokine, interleukin-1 (IL-1), has been investigated. Mouse thymoma (EL4 6.1) cells were preincubated with [3H]-glycerol and then incubated with recombinant IL-1 beta for varying periods. Interleukin-1 caused a rapid increase in diacylglycerol production (approx. 2 fold at 30 secs). This reproducible enhancement of diacylglycerol accumulation was abolished by pretreatment of the cells with pertussis toxin. Interestingly, a similar IL-1 induced increase in diacylglycerol was observed when the cells were preincubated with [3H]-myristic acid. These results appear to suggest a novel mode of action of interleukin-1 which involves a G-protein mediated breakdown of a membrane lipid resulting in the production of diacylglycerol. It is suggested that one possible candidate for this parent lipid may be a phosphatidylinositol glycan.
Dobson
P R
PR
Department of Human Metabolism & Clinical Biochemistry, University of Sheffield Medical School, United Kingdom.
Plested
C P
CP
Jones
D R
DR
Barks
T
T
Brown
B L
BL
D000818:Animals; D004075:Diglycerides / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects; D005989:Glycerides / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000494:pharmacology*; D051379:Mice; D037342:Pertussis Toxin; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D013945:Thymoma / Q000378:metabolism / Q000473:pathology*; D013953:Thymus Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D004075:Diglycerides; D005989:Glycerides; D007375:Interleukin-1; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin