Monocyte-mediated suppression of anti-DNA antibody production in healthy subjects.
Monocyte-depleted mononuclear cells (MNC) from healthy subjects synthesized IgM-class antibody to single-stranded DNA (anti-ssDNA) at significantly higher levels than unfractionated MNC. The increased production of IgM anti-ssDNA by monocyte-depleted normal MNC was inhibited to a greater degree than total IgM by addition of supernatants from autologous monocytes. Moreover, supernatants obtained from normal monocytes cultured with indomethacin retained this suppressive effect on IgM anti-ssDNA antibody production, suggesting that prostaglandin E2 may not be involved in the suppression. These results indicate that B cells programmed to produce IgM anti-ssDNA are present in the normal B cell repertoire, but may be suppressed by monocytes or by a soluble factor (or factors) from the monocytes.
Okawa-Takatsuji
M
M
Division of Immunology, National Medical Center, Tokyo, Japan.
Aotsuka
S
S
Uwatoko
S
S
Kinoshita
M
M
Yokohari
R
R
D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D004277:DNA, Single-Stranded / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis*; D009000:Monocytes / Q000502:physiology*; D012016:Reference Values; D012217:Rheumatoid Factor / Q000096:biosynthesis
D000974:Antibodies, Antinuclear; D004277:DNA, Single-Stranded; D007075:Immunoglobulin M; D012217:Rheumatoid Factor