Abnormal development of B cells and B cell progenitors in autoimmune (NZB x NZW)F1 mice.
The (NZB x NZW)F1 (BWF1) autoimmune strain displays reduced numbers of both c mu+ pre-B cells and Ly5(220)+ B cell progenitors in the bone marrow. The loss of these B cell precursor populations in the bone marrow increases with age. In contrast, the bone marrow of BWF1 mice possesses sIg+ B cells comparable in both number and surface phenotype to that observed in conventional strains. Analysis of the surface densities of both sIg and Ly5(220) antigens indicates that BWF1 bone marrow B cells comprise a heterogeneous population of both immature and mature B cells. In addition, BWF1 bone marrow still possessed progenitor cells capable of yielding newly generated B cell precursors and B cells in vitro. The diminished levels of intermediate B cell progenitors observed in BWF1 bone marrow may reflect abnormal regulation of B lineage differentiation during the life span of this autoimmune strain.
Riley
R L
RL
Department of Microbiology and Immunology, University of Miami School of Medicine, Florida 33136.
Kruger
M G
MG
Riley
E A
EA
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D001327:Autoimmune Diseases / Q000276:immunology / Q000473:pathology*; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003433:Crosses, Genetic; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000276:immunology / Q000473:pathology*; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell
D000950:Antigens, Ly; D011947:Receptors, Antigen, B-Cell