Membrane fluidity affects tumor-cell motility, invasion and lung-colonizing potential.
Membrane fluidity, determined by steady-state fluorescence polarization measurements, was correlated with metastatic capacity of murine tumor-cell lines. A correlation was observed in cell lines with different metastatic potential, and was confirmed when their lung-colonizing ability was modulated by alteration of either the membrane lipid composition or the culture conditions. Two cellular functions, motility and basement membrane invasion, were affected by the membrane lipid composition, and might explain the role of membrane fluidity observed in cancer metastasis.
Taraboletti
G
G
Istituto di Ricerche Farmacologiche Mario Negri, Bergamo, Italy.
Perin
L
L
Bottazzi
B
B
Mantovani
A
A
Giavazzi
R
R
Salmona
M
M
D000818:Animals; D001485:Basement Membrane / Q000503:physiopathology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology; D005260:Female; D005454:Fluorescence Polarization; D008175:Lung Neoplasms / Q000503:physiopathology / Q000556:secondary*; D008560:Membrane Fluidity / Q000502:physiology*; D008563:Membrane Lipids / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
D008563:Membrane Lipids