Post-metaphase mitotic events in cells treated with dinitrophenol.
The sequence of post-metaphase mitotic events, such as anaphase movement A and B, chromosome decondensation, nuclear envelope reformation and cytokinesis, has been studied in 2,4-initrophenol (DNP)-treated HeLa cells. The effects of DNP were found to be dose dependent and at concentrations higher than 3 mM, both anaphase A and B movements were totally and nearly instantaneously arrested. It could be shown that cytokinesis did not depend on the completion of anaphase movements. This was also true for nuclear envelope reformation which could take place even around condensed chromosomes arrested in anaphase. The post-metaphase mitotic events do not follow a strict causal sequence, but they can be dissociated from each other in anaphase-arrested cells.
Ghosh
S
S
Paweletz
N
N
Armas-Portela
R
R
D019297:2,4-Dinitrophenol; D000705:Anaphase / Q000187:drug effects; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure*; D004140:Dinitrophenols / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D008677:Metaphase / Q000187:drug effects; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects*; D009685:Nuclear Envelope / Q000187:drug effects / Q000648:ultrastructure
D004140:Dinitrophenols; D019297:2,4-Dinitrophenol