Antiviral effect of lymphokine-activated killer cells: chemotaxis and homing to sites of virus infection.
Lymphokine-activated killer (LAK) cells generated from C57BL/6 mouse spleen cells cultured with interleukin-2 are effective prophylactically against virus infection when inoculated at the site of virus injection. To predict the therapeutic efficacy of LAK cells, we determined whether LAK cells would home to sites of virus infection. In vitro, LAK cells responded chemotactically to cell-free peritoneal exudate fluids collected from virus-infected mice and to preparations of purified beta interferon. In vivo, radiolabeled LAK cells injected intravenously accumulated in the peritoneal cavities of intraperitoneally infected mice in amounts three to eight times greater than in uninfected mice. This ability to respond to chemotactic agents and migrate into sites of virus infection may make LAK cells useful as antiviral therapeutic agents.
Natuk
R J
RJ
Department of Pathology, University of Massachusetts Medical School, Worcester 01655.
Bukowski
J F
JF
Brubaker
J O
JO
Welsh
R M
RM
D000818:Animals; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D007370:Interferon Type I / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D015979:Killer Cells, Lymphokine-Activated / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000276:immunology; D014616:Vaccinia virus / Q000502:physiology*
D007370:Interferon Type I; D007376:Interleukin-2; D011994:Recombinant Proteins