title: Characterization of the virA virulence gene of the nopaline plasmid, pTiC58, of Agrobacterium tumefaciens.
abstract: We have determined the complete nucleotide sequence of a 4.8 kilobase fragment encompassing the virA locus of the nopaline-type plasmid, pTiC58, of Agrobacterium tumefaciens. virA is composed of a single open reading frame of 2499 nucleotides, capable of encoding a protein of 91.3 kiloDaltons. A trpE::virA gene fusion was used to confirm the reading frame of virA. High nucleotide and amino acid sequence homologies were observed between pTiC58 virA and the virA sequences of three octopine-type plasmids. Strong homologies in amino acid sequence were observed between pTiC58 VirA and seven bacterial proteins which control various regulons. Two hydrophobic domains within VirA are also consistent with a model in which VirA acts as a membrane-bound sensor of plant signal molecules.
authors: Davis Crown Gall Group, Department of Plant Pathology, University of California, Davis 95616.
authors: P
authors: P
authors: Morel
authors: B S
authors: BS
authors: Powell
authors: P M
authors: PM
authors: Rogowsky
authors: C I
authors: CI
authors: Kado
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000235:genetics / Q000031:analogs & derivatives; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics* / Q000472:pathogenicity; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C008666:nopaline; C005394:octopine; D001120:Arginine