SV40 T antigen acts as a minor histocompatibility antigen of SV40 T antigen tolerant transgenic mice.
The ability of normal mice to mount an SV40 T antigen-specific cytolytic T lymphocytes response when immunized in vivo with splenocytes from the SV40 T antigen transgenic 427-line mice and restimulated in vitro with SV40-transformed fibroblasts, or when immunized with SV40 and restimulated with 427-line splenocytes, was analyzed. Both immunization schemes resulted in an SV40 T antigen-specific immune response, indicating the presence of SV40 T antigen-positive cells in the spleens of these transgenic mice. Normal mice engrafted with skin from 427 donors showed no rejection of the graft. Thus, SV40 T antigen in transgenic 427-line mice is expressed on an undefined cell type in the spleen and acts as a tissue-specific minor histocompatibility antigen.
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Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
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D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000276:immunology*; D002461:Cell Line, Transformed; D003602:Cytotoxicity, Immunologic; D007108:Immune Tolerance; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic / Q000276:immunology*; D008912:Minor Histocompatibility Loci; D013154:Spleen; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000952:Antigens, Polyomavirus Transforming