title: Glycine tRNA mutants with normal anticodon loop size cause -1 frameshifting.
abstract: Mutations in the acceptor stem, the 5-methyluridine-pseudouridine-cytidine (TFC) arm, and the anticodon of Salmonella tRNA2Gly can cause -1 frameshifting. The potential for standard base pairing between acceptor stem positions 1 and 72 is disrupted in the mutant sufS627. This disruption may interfere with the interaction of the tRNA with elongation factor-Tu.GTP or an as-yet-unspecified domain of the ribosome. The potential for standard base pairing in part of the TFC stem is disrupted in mutant sufS625. The nearly universal C-61 base of the TFC stem is altered in mutant sufS617, and the TFC loop is extended in mutant sufS605. These changes are expected to interfere with the stability of the TFC loop and its interaction with the D arm. The mutation in mutant sufS605, and possibly other mutants, alters nucleoside modification in the D arm. Three mutants, sufS601, sufS607, and sufS609, have a cytidine substituted for the modified uridine at position 34, the first anticodon position. None of the alterations grossly disrupts in-frame triplet decoding by the mutant tRNAs. The results show that -1 frameshifting in vivo can be caused by tRNAs with normal anticodon loop size and suggest that alternative conformational states of the mutant tRNAs may allow them to read a codon in frame or to shift reading frame.
authors: Department of Biochemistry, University College, Cork, Ireland.
authors: D J
authors: DJ
authors: O'Mahony
authors: B H
authors: BH
authors: Mims
authors: S
authors: S
authors: Thompson
authors: E J
authors: EJ
authors: Murgola
authors: J F
authors: JF
authors: Atkins
mesh_terms: D000926:Anticodon / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000235:genetics*; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012353:RNA, Transfer, Gly / Q000235:genetics*; D012475:Salmonella / Q000235:genetics*
chemical_list: D000926:Anticodon; D004269:DNA, Bacterial; D012345:RNA, Transfer, Amino Acid-Specific; D012353:RNA, Transfer, Gly; D012343:RNA, Transfer