Bovine tooth-derived bone morphogenetic protein.
Eight groups of dental tissues were mechanically dissected from the mandibles of one-year-old steers; they were then defatted and decalcified in HCl. The noncollagenous proteins were extracted with various solvents from collections of tissue and bio-assayed for osteo-inductive activity. Collectively, the hard tissue (dentin, enamel, and cementum) noncollagenous proteins were fractionated by molecular sieve chromatography, hydroxyapatite affinity chromatography, and ion exchange chromatography. Osteo-inductive activity of each protein fraction was determined by implantation in the quadriceps muscle pouch of mice. The quantity of bone was measured by computerized image analysis. From 71% to 83% of 41 implants of dental hard tissues induced bone formation. The quantity of bone was greater from unerupted than from erupted teeth. Dental soft tissues that had no osteo-inductive activity were rich in a 14-kDa protein, presumably matrix gamma-carboxyglutamic acid-rich proteins. Proteins with Mr of from 15 to 28 kDa were associated with osteo-inductive activity. Components with Mr greater than 28 kDa had no activity. These observations suggest that bovine teeth have a selection of osteo-inductive proteins that is comparable in range of MW to bovine bone morphogenetic protein.
Kawai
T
T
UCLA Bone Research Laboratory, 90024-1790.
Urist
M R
MR
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D019485:Bone Morphogenetic Proteins; D002417:Cattle; D003739:Dental Cementum / Q000032:analysis; D003743:Dental Enamel / Q000032:analysis; D003804:Dentin / Q000032:analysis; D006133:Growth Substances / Q000302:isolation & purification; D051379:Mice; D011506:Proteins; D014070:Tooth / Q000032:analysis*; D014083:Tooth Germ / Q000032:analysis*; D014097:Tooth, Unerupted / Q000032:analysis*
D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D011506:Proteins