Interleukin-6 (IL-6) functions as an in vitro autocrine growth factor in renal cell carcinomas.
Interleukin-6 (IL-6) was found to be a growth factor of renal cell carcinomas Furthermore, renal cell carcinomas freshly isolated from the patients expressed mRNA of IL-6 and secreted biologically active IL-6 under the culture conditions where the tumor cells could grow, but they did not produce IL-6 nor proliferate in the absence of fetal calf serum. The production of IL-6 by the tumor cells was also demonstrated by immunostaining of the IL-6-producing cells utilizing anti-IL-6 antiserum. Moreover, anti-IL-6 antiserum specifically inhibited the in vitro tumor growth. All data indicated that IL-6 functions as an in vitro autocrine growth factor of renal cell carcinomas.
Miki
S
S
Third Department of Internal Medicine, Nissei Hospital, Osaka, Japan.
Iwano
M
M
Miki
Y
Y
Yamamoto
M
M
Tang
B
B
Yokokawa
K
K
Sonoda
T
T
Hirano
T
T
Kishimoto
T
T
D015152:Blotting, Northern; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000503:physiopathology*; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D015850:Interleukin-6; D007378:Interleukins / Q000235:genetics / Q000502:physiology*; D007680:Kidney Neoplasms / Q000235:genetics / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics
D006133:Growth Substances; D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger