Inhibition of in vivo DNA synthesis in regenerating rat liver following thermal injury.
Hepatic repair and regeneration which is extremely important after thermal injuries can be inhibited by the acute inflammatory reaction. Since thermal injury initiates this acute inflammatory reaction, DNA synthesis was studied in the regenerating liver following this injury. In vivo incorporation of [3H]-thymidine into hepatic DNA, autoradiographic determination of a labeling index, and thymidine kinase activity were determined. Incorporation of [3H]-thymidine into hepatic DNA and labeling indices were markedly diminished at 24 hours if partial hepatectomy and thermal injury were carried out concurrently. After partial hepatectomy, the expected elevations in thymidine kinase activity were inhibited by the thermal injury (p less than 0.01) and elevation of serum fibrinogen, a marker of the acute phase reaction that normally follows thermal injury, was blunted by the partial hepatectomy (p less than 0.05). The combination of thermal injury and partial hepatectomy resulted in a greatly diminished DNA replicative response as compared to partial hepatectomy alone and suggests that multiplicative injury is more likely to result in multi-system failure.
Carter
E A
EA
Department of Pediatrics, Harvard Medical School, Boston, MA 02114.
Kirkham
S E
SE
Tompkins
R G
RG
Burke
J F
JF
D000818:Animals; D002056:Burns / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008115:Liver Regeneration; D051381:Rats; D011917:Rats, Inbred Lew; D013937:Thymidine Kinase / Q000378:metabolism
D005340:Fibrinogen; D004247:DNA; D013937:Thymidine Kinase