Structure, organization, and expression of the rat cardiac myosin light chain-2 gene. Identification of a 250-base pair fragment which confers cardiac-specific expression.
The present study characterized the structure, organization, and expression of the rat cardiac myosin light chain (MLC) -2 gene. The rat cardiac MLC-2 gene has seven exons which display complete conservation with the exon structure of the rat fast twitch skeletal MLC-2 gene. A 250-base pair (bp) sequence of the 5'-flanking region contains CArG motifs and additional cis elements, each greater than 10 bp in length, which were conserved in sequence and relative position with the chick cardiac MLC-2 gene. A series of MLC-2/luciferase fusion genes consisting of nested 5' deletions of the MLC-2 5'-flanking region were constructed and transfected into primary neonatal rat myocardial cells and a non-myocardial cell line (CV-1), demonstrating that this 250 bp of the MLC-2 5'-flanking region was sufficient to confer cardiac specific expression on a luciferase reporter gene. This study suggests the presence of important proximal regulatory sequences in the MLC-2 5'-flanking region which are capable of directing the cardiac specific expression of the rat cardiac myosin light chain-2 gene.
Henderson
S A
SA
Department of Medicine, University of California, San Diego, School of Medicine, La Jolla 92093.
Spencer
M
M
Sen
A
A
Kumar
C
C
Siddiqui
M A
MA
Chien
K R
KR
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001482:Base Composition; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005091:Exons; D015870:Gene Expression; D005796:Genes; D006352:Heart Ventricles / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D015879:Myosin Subfragments / Q000235:genetics*; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D015879:Myosin Subfragments