Plasma DNA in patients undergoing hemodialysis or hemofiltration: cytolysis in artificial kidney is responsible for the release of DNA in circulation.
Levels of circulating DNA increase under treatment by an artificial kidney. Using a new assay, levels of plasma DNA are studied in 45 patients during 99 sessions of hemodialysis or hemofiltration. Before the session, plasma DNA levels are increased in 41/99 samples and, among them, in 18/24 samples collected from hepatitis B surface antigen carriers. During the first 3 h of the session, plasma DNA levels increase whatever the method of treatment. At the 30th and 60th minute of hemodialysis, a positive gradient of plasma DNA exists between the output and the input of the artificial kidney. It is concluded that: (1) the increase in plasma DNA is related to the overall procedure of artificial kidney therapy; (2) death of leukocytes in the artificial kidney is responsible for the release and the increase in circulation of extracellular DNA.
Fournié
G J
GJ
Laboratoire d'Immunopathologie Rénale et d'Immunopharmacologie CJF INSERM 1, Centre Hospitalier La Grave, Toulouse, France.
Lulé
J
J
Dueymes
J M
JM
Laval
F
F
Delobbe
I
I
Vernier
I
I
Pourrat
J P
JP
D002470:Cell Survival; D004247:DNA / Q000097:blood*; D005260:Female; D006440:Hemofiltration; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy; D007688:Kidneys, Artificial; D007962:Leukocytes / Q000502:physiology; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis
D006514:Hepatitis B Surface Antigens; D004247:DNA