Role of mast cells in anaphylaxis. Evidence for the importance of mast cells in the cardiopulmonary alterations and death induced by anti-IgE in mice.
We used genetically mast cell-deficient WBB6F1-W/Wv and WCB6F1-S1/S1d mice and the congenic normal (+/+) mice to investigate the effects of intravenous infusion of goat antimouse IgE on heart rate (HR), pulmonary dynamic compliance (Cdyn), pulmonary conductance (GL), and survival. In WBB6F1-+/+ and WCB6F1-+/+ mice, anti-IgE induced extensive degranulation of tracheobronchial mast cells, as well as significant elevation of HR, significant reductions in Cdyn and GL and, in some cases, death. In contrast, W/Wv and S1/S1d mice exhibited little or no pathophysiological responses and no mortality after challenge with anti-IgE. In W/Wv mice reconstituted with mast cells by intravenous administration of bone marrow cells derived from congenic +/+ mice (+/+ BM----W/Wv mice), anti-IgE induced extensive mast cell degranulation, as well as pathophysiological responses and mortality similar to those observed in WBB6F1-+/+ mice. These findings suggest a critical role for mast cells in the development of the cardiopulmonary changes and mortality associated with anti-IgE-induced anaphylaxis.
Martin
T R
TR
Department of Pediatrics, Children's Hospital, Boston, Massachusetts.
Galli
S J
SJ
Katona
I M
IM
Drazen
J M
JM
D000707:Anaphylaxis / Q000276:immunology* / Q000401:mortality / Q000473:pathology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage*; D001980:Bronchi / Q000473:pathology; D006321:Heart / Q000503:physiopathology*; D006801:Humans; D007073:Immunoglobulin E / Q000008:administration & dosage / Q000276:immunology*; D007074:Immunoglobulin G / Q000008:administration & dosage; D008168:Lung / Q000503:physiopathology*; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D014132:Trachea / Q000473:pathology
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; C026408:anti-IgE antibodies; D007073:Immunoglobulin E