title: The expression of acetylated microtubules during axonal and dendritic growth in cerebellar macroneurons which develop in vitro.
abstract: The distribution of acetylated microtubules in cerebellar macroneurons which develop in culture was studied with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. In these neurons 6-11B-1 helps to define a subset of stable, colchicine-resistant, microtubules that during early neuronal development are exclusively localized in the neuron's axon. On the other hand, in mature neurons acetylated microtubules display a widespread distribution being localized in the axon and thick dendritic trunks, a phenomenon correlated with an increase in the levels of acetylated alpha-tubulin and colchicine-resistant microtubules. Taken collectively, these observations suggest that the acetylation of alpha-tubulin is important for differentiating microtubules during neurite growth: in young neurons this post-translational modification may contribute to determine a selective stabilization of microtubules accompanying axonal differentiation.
authors: Laboratorio de Neurobiología, Instituto de Investigación Médica Mercedes y Martín Ferreyra, Córdoba, Argentina.
authors: A
authors: A
authors: Ferreira
authors: A
authors: A
authors: Cáceres
mesh_terms: D000107:Acetylation; D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000378:metabolism* / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism* / Q000502:physiology; D008870:Microtubules / Q000378:metabolism*; D051381:Rats; D014404:Tubulin / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D014404:Tubulin