title: Analysis of the alpha-amylase gene of Schwanniomyces occidentalis and the secretion of its gene product in transformants of different yeast genera.
abstract: We have cloned and characterized the alpha-amylase gene (AMY1) of the yeast Schwanniomyces occidentalis. A cosmid gene library of S. occidentalis DNA was screened in Saccharomyces cerevisiae for alpha-amylase secretion. The positive clone contained a DNA fragment harbouring an open reading frame of 1536 nucleotides coding for a 512-amino-acid polypeptide with a calculated Mr of 56,500. The deduced amino acid sequence reveals significant similarity to the sequence of the Saccharomycopsis fibuligera and Aspergillus oryzae alpha-amylases. The AMY l gene was found to be expressed from its original promoter in S. cerevisiae, Kluyveromyces lactis and Schizo-saccharomyces pombe leading to an active secreted gene product and thus enabling the different yeast transformants to grow on starch as a sole carbon source.
authors: Rhein Biotech GmbH, Düsseldorf, Federal Republic of Germany.
authors: A W
authors: AW
authors: Strasser
authors: R
authors: R
authors: Selk
authors: R J
authors: RJ
authors: Dohmen
authors: T
authors: T
authors: Niermann
authors: M
authors: M
authors: Bielefeld
authors: P
authors: P
authors: Seeboth
authors: G H
authors: GH
authors: Tu
authors: C P
authors: CP
authors: Hollenberg
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D014170:Transformation, Genetic; D015003:Yeasts / Q000201:enzymology*; D000516:alpha-Amylases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
chemical_list: D000516:alpha-Amylases