title: Human immunosuppressive factors produced by T cell hybridoma.
abstract: A Human OKT8-positive T cell hybridoma was established by using concanavalin A (ConA)-activated peripheral blood mononuclear cells (PBMC) fused with CEM-AGR cells. A culture supernatant of the established T cell hybridoma HI4.2D6 inhibited the mitogen induced proliferation of peripheral blood mononuclear cells (PBMC). It inhibited IL-1-, IL-2, or IL-3-dependent proliferation of each factor-dependent mouse cell lines. While the inhibitory activity of IL-1-induced proliferation was stable, that of IL-2- and IL-3-induced proliferation was abrogated within a week at 4 degrees C. Inhibition of IL-2-dependent growth was also observed using human IL-2-dependent cells, but the growth of other factor-independent human hematopoietic cell lines was not affected at all. By gel filtration, the activity inhibiting IL-2- or IL-3-dependent proliferation was found in the fractions with approximate molecular weight of 18,000 and 100,000.
authors: Department of Cellular Immunology, National Institute of Health, Tokyo, Japan.
authors: Y
authors: Y
authors: Tsunetsugu-Yokota
authors: M
authors: M
authors: Honda
authors: T
authors: T
authors: Tokunaga
mesh_terms: D002460:Cell Line; D006131:Growth Inhibitors; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D006131:Growth Inhibitors; D007376:Interleukin-2; D008934:Mitogens; D013491:Suppressor Factors, Immunologic