title: Chemical and immunochemical characterization of polymers of aggregates in preparations of human serum albumin.
abstract: The polymer/aggregate fractions of three human serum albumin preparations (HSA) from different manufacturers were isolated by size-exclusion high-performance liquid chromatography (HPLC) and analyzed by different electrophoretic and immunochemical methods. All of these polymer/aggregate fractions contained only 30-50% albumin. The rest seemed to be mainly heat-denatured haptoglobin which did not react with an anti-haptoglobin serum, whereas the albumin part reacted with an anti-albumin serum. It could be shown that these polymers or aggregates are formed by disulfide bonds between albumin and the small amounts of denatured impurity globulins (haptoglobin, transferrin) during the pasteurization step. The higher the amount of these heat labile globulins in the final preparation before the pasteurization step, the higher was the polymer/aggregate content of the pasteurized albumin preparation.
authors: DRK-Blutspendezentrale Baden-Baden.
authors: T
authors: T
authors: Dengler
authors: U
authors: U
authors: Stöcker
authors: S
authors: S
authors: Kellner
authors: G
authors: G
authors: Fürst
mesh_terms: D001797:Blood Protein Electrophoresis; D001803:Blood Transfusion; D002851:Chromatography, High Pressure Liquid; D006242:Haptoglobins / Q000032:analysis; D006361:Heating; D006801:Humans; D011108:Polymers / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis*; D014168:Transferrin / Q000032:analysis
chemical_list: D006242:Haptoglobins; D011108:Polymers; D012709:Serum Albumin; D014168:Transferrin