Estimation of local cerebral protein synthesis rates with L-[1-11C]leucine and PET: methods, model, and results in animals and humans.
We have estimated the cerebral protein synthesis rates (CPSR) in a series of normal human volunteers and monkeys using L-[1-11C]leucine and positron emission tomography (PET) using a three-compartment model. The model structure, consisting of a tissue precursor, metabolite, and protein compartment, was validated with biochemical assay data obtained in rat studies. The CPSR values estimated in human hemispheres of about 0.5 nmol/min/g agree well with hemispheric estimates in monkeys. The sampling requirements (input function and scanning sequence) for accurate estimates of model parameters were investigated in a series of computer simulation studies.
Hawkins
R A
RA
Department of Radiological Sciences, UCLA School of Medicine, Los Angeles, CA 90024.
Huang
S C
SC
Barrio
J R
JR
Keen
R E
RE
Feng
D
D
Mazziotta
J C
JC
Phelps
M E
ME
D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000097:blood; D004032:Diet; D006801:Humans; D007700:Kinetics; D007930:Leucine / Q000097:blood; D008253:Macaca mulatta; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051381:Rats; D014055:Tomography, Emission-Computed / Q000379:methods
D002250:Carbon Radioisotopes; D009419:Nerve Tissue Proteins; D007930:Leucine