title: Calcium-dependent dynamic changes in the subcellular distribution of calmodulin in frog spinal cells.
abstract: Calcium-dependent changes in the subcellular distribution of calmodulin (CaM) were investigated using isolated intraarterially perfused frog spinal cords. Perfusion with quin2-tetraacetoxymethyl ester (Q2-AM), an intracellular Ca2+-chelator, increased soluble CaM, measured by radioimmunoassay (RIA), with a concomitant decrease in particulate CaM. By contrast, in synaptosomes, Q2-AM increased soluble CaM and total CaM but not particulate CaM. These results suggest that intracellular Ca2+ controls the binding of CaM to membranes in spinal cells.
authors: Department of Toxicology and Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors: S
authors: S
authors: Adachi
authors: J I
authors: JI
authors: Oka
authors: H
authors: H
authors: Fukuda
mesh_terms: D000634:Aminoquinolines / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002147:Calmodulin / Q000378:metabolism*; D002614:Chelating Agents / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D011892:Rana catesbeiana; D012995:Solubility; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D000634:Aminoquinolines; D002147:Calmodulin; D002614:Chelating Agents; C035738:Quin2-acetoxymethyl ester; D002118:Calcium