Comparative effects of T-2 toxin and diacetoxyscirpenol on drug metabolizing enzymes in rat tissues.
The effects of T-2 toxin and diacetoxyscirpenol on tissue drug-metabolizing enzymes in young male rats were compared. Mycotoxicoses were produced by daily oral administration of toxins at 1.0 mg/kg body weight for 1, 4 or 8 days. Many hepatic, renal and pulmonary oxidative and conjugative enzymes were measured in animals killed 24 hr following the last administration. The effects of the two trichothecene mycotoxins were generally similar. In liver the decrease in microsomal and cytosolic proteins paralleled the decline in total plasma proteins or the increase in plasma GOT activity. Hepatic microsomal cytochrome P-450 decreased in rats receiving trichothecenes for 8 days. This effect was more marked when aminopyrine, benzphetamine, ethylmorphine and ethoxycoumarin dealkylations or aniline and benzopyrene hydroxylations were measured. p-nitrophenol glucuronyltransferase activity was enhanced in animals receiving at least one administration of trichothecenes, whereas there was no change in conjugation to glutathione or acetate. In other tissues, there was no change in any renal enzymes whereas a significant rise in pulmonary monooxygenase was observed in T-2 toxin administered to rats for 4 or 8 days.
Galtier
P
P
Laboratoire de Pharmacologie-Toxicologie, INRA, Toulouse, France.
Paulin
F
F
Eeckhoutte
C
C
Larrieu
G
G
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D065607:Cytochrome P-450 Enzyme Inhibitors; D003600:Cytosol / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008168:Lung / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology; D009183:Mycotoxins / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000494:pharmacology*; D013255:Steroid 21-Hydroxylase / Q000378:metabolism; D013605:T-2 Toxin / Q000494:pharmacology*; D014255:Trichothecenes / Q000494:pharmacology*
D065607:Cytochrome P-450 Enzyme Inhibitors; D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; D013255:Steroid 21-Hydroxylase; D001219:Aspartate Aminotransferases; D013605:T-2 Toxin; C001809:diacetoxyscirpenol