Treatment of Brucella melitensis infection in mice by use of liposome-encapsulated gentamicin.
Liposomes with entrapped gentamicin were used to treat mice with infection attributable to Brucella melitensis. Liposomes bearing positive charge and formed by egg yolk lecithin, cholesterol, and stearylamine were effective in the elimination of B melitensis residing in liver and spleen. Negatively charged liposomes, formed by egg yolk lecithin, cholesterol, and dicetyl phosphate were also effective in suppression of the infection in liver, but were less so in suppression of the infection in the spleen. Free gentamicin was less effective than the encapsulated antibiotic. At 20 hours after administration of gentamicin encapsulated in liposomes, the gentamicin concentrations in liver and spleen were similar, regardless of the charge of the liposomes--neutral, positive, or negative. However, positively charged liposomes were more efficient than were other liposome types for the treatment of brucellosis caused by B melitensis.
Hernández-Caselles
T
T
Departamento de Bioquímica, Facultad de Veterinaria, Universidad de Murcia, Spain.
Vera
A
A
Crespo
F
F
Villalaín
J
J
Gómez-Fernández
J C
JC
D000588:Amines; D000818:Animals; D002002:Brucella / Q000302:isolation & purification; D002006:Brucellosis / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D002784:Cholesterol; D004337:Drug Carriers; D005839:Gentamicins / Q000008:administration & dosage* / Q000627:therapeutic use; D008081:Liposomes; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D010713:Phosphatidylcholines
D000588:Amines; D004337:Drug Carriers; D005839:Gentamicins; D008081:Liposomes; D010713:Phosphatidylcholines; D002784:Cholesterol; C009317:stearylamine