title: Isolation and structure of ranamargarin, a new tachykinin from the skin of Chinese frog Rana margaratae.
abstract: A new tetradecapeptide, ranamargarin, has been isolated by Sep-Pak C18 and HPLC from methanol extracts of the skin of the Chinese frog Rana margaratae. The sequence of the peptide is: Asp-Asp-Ala-Ser-Asp-Arg-Ala-Lys-Lys-Phe-Tyr-Gly-Leu-Met-NH2. This structure has been confirmed by synthesis. The peptide is the largest among the amphibian tachykinins and its N-terminal amino acids are quite different from those of the other tachykinins. The formation of the sulfoxide and peak-splitting of ranamargarin during purification procedures are briefly discussed.
authors: Shanghai Institute of Materia Medica, Academia Sinica.
authors: Y Q
authors: YQ
authors: Tang
authors: S H
authors: SH
authors: Tian
authors: S X
authors: SX
authors: Wu
authors: J C
authors: JC
authors: Hua
authors: G F
authors: GF
authors: Wu
authors: E M
authors: EM
authors: Zhao
authors: Y A
authors: YA
authors: Lu
authors: Y Q
authors: YQ
authors: Zhu
authors: G
authors: G
authors: Zou
authors: K
authors: K
authors: Tsou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D011898:Ranidae; D012867:Skin / Q000032:analysis*; D015320:Tachykinins / Q000302:isolation & purification*
chemical_list: D015320:Tachykinins; C061715:ranamargarin