[Identification of a peptide of the membrane protein of tobacco mosaic virus, cross-linked with intraviral RNA under the effect of UV-radiation].
As reported previously, UV-irradiation induces crosslinking between tobacco mosaic virus (TMV) coat protein molecules and intraviral RNA nucleotides. We have irradiated [3H]-uridine labeled TMV and isolated TMV coat protein subunits with the attached nucleotide label. These TMV protein subunits were hydrolyzed with trypsin. The tryptic peptides were separated by high-performance liquid chromatography and [3H]-labeled peptides were identified. The UV-irradiation of TMV was found to result in crosslinking to intraviral RNA of the T8 tryptic peptide (residues 93-112) of TMV coat protein.
Grebenshchikov
N I
NI
Arbieva
Z Kh
ZKh
Dobrov
E N
EN
D002213:Capsid / Q000378:metabolism* / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D010455:Peptides / Q000032:analysis*; D012367:RNA, Viral / Q000378:metabolism* / Q000528:radiation effects; D014027:Tobacco Mosaic Virus / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D010455:Peptides; D012367:RNA, Viral
Identifikatsiia peptida belka obolochki virusa tabanoĭ mozaiki, sshivaiushchegosiia s vnutrivirusnoĭ RNK pod deĭstviem UF- izlucheniia.