Non-contiguous regions of Z-DNA in a DNA dodecamer.
The conformation of the self-complimentary DNA dodecamer d(br5CGbr5CGAATTbr5CGbr5CG) has been investigated in a variety of salt and solvent conditions by one and two-dimensional 1H NMR. In low salt aqueous solutions, the molecule forms a regular B-DNA structure similar to the unmodified dodecamer. However, in aqueous solution containing high salt concentration and methanol, the dodecamer adopts a structure in which the br5CGbr5CG ends of the molecule are in a Z-DNA like conformation and the AATT region is neither standard B-DNA nor Z-DNA. The implications of these results for the structure of junctions between B and Z-DNA and the sequence specificity of Z-DNA are discussed.
Hua
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Department of Chemistry & Biochemistry, University of California, Los Angeles 90024-1569.
van der Marel
G A
GA
van Boom
J H
JH
Feigon
J
J
D001483:Base Sequence; D004247:DNA; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D012997:Solvents
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D012997:Solvents; D004247:DNA