Immunoassay by particle counting for coagulation testing: application to the determination of antithrombin III, von Willebrand factor antigen (vWF:Ag) and plasminogen.
Based on immunoassay by particle counting, three methods for antithrombin III, von Willebrand factor and plasminogen were developed on an automated IMPACT machine and on a semi-automated MULTIPACT system. Precision of the techniques, measured at low, medium and high level of the calibration curve showed coefficients of variation varying from 4.3 to 13.8%. Accuracy was evaluated by dilution recovery test and by correlation with rocket immunoelectrophoresis and chromogenic substrate techniques. The results show that the proposed methods correlate well with existing techniques and that immunoassay by particle counting is applicable to several coagulation tests.
Borque
L
L
Unit of Experimental Medicine, International Institute for Cellular and Molecular Pathology, Bruxelles, Belgium.
Cambiaso
C
C
Lavenne
E
E
Leautaud
P
P
Mareschal
J C
JC
Collet-Cassart
D
D
D000941:Antigens / Q000032:analysis*; D000990:Antithrombin III / Q000032:analysis; D001780:Blood Coagulation Tests / Q000379:methods; D006801:Humans; D010958:Plasminogen / Q000032:analysis*; D014841:von Willebrand Factor / Q000032:analysis*
D000941:Antigens; D014841:von Willebrand Factor; D000990:Antithrombin III; D010958:Plasminogen