Growth requirements of T cell hybridomas obtained by the fusion between a mouse cytolytic T cell line and the rat tumor C58NTD.
T23 are hybrids derived from the fusion between an IL-2-dependent mouse cell line, C10 and the rat lymphoma C58NTD. Supernatants from exponentially growing T23 cells induce the growth of CTLL2, and IL-2-dependent cell line, suggesting that these hybrids secrete interleukin 2. Addition of recombinant IL-2 to slowly growing T23 cells increases the rate of growth. Using an 125I IL-2 binding assay, a low number of cell surface IL-2 receptors were detected. T23 hybrids contain mouse but not rat IL-2 receptor genes as revealed by Southern blot analysis. These receptors are functional because the growth of exponentially growing hybrids is inhibited by an anti-mouse IL-2 receptor antibody. These data suggest an autocrine-like mechanism as responsible for the growth of these T cell hybridomas.
Silva
A
A
Centro de Investigaciones Biologicas, CSIC, Madrid, Spain.
Fernandez-Ruiz
E
E
Sanz
E
E
Somoza
C
C
D000818:Animals; D015139:Blotting, Southern; D002455:Cell Division; D002459:Cell Fusion; D003602:Cytotoxicity, Immunologic; D006825:Hybridomas / Q000166:cytology*; D007111:Immunity, Cellular; D007376:Interleukin-2 / Q000096:biosynthesis; D051379:Mice; D051381:Rats; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology*
D007376:Interleukin-2; D015375:Receptors, Interleukin-2