DNA damage in mouse and rat liver by caprolactam and benzoin, evaluated with three different methods.
Benzoin and caprolactam were examined for their capability of inducing alkaline DNA fragmentation in mouse and rat liver DNA after treatment in vivo. Three different methods were used. With the alkaline elution technique we measured an effect presumably related to the conformation of the DNA coil. With a viscometric and a fluorometric unwinding method we measured an effect presumably related to the number of unwinding points in DNA. For both compounds only the alkaline elution technique was clearly positive. The results suggest that both caprolactam and benzoin can induce an important change in the conformation of the DNA coil without inducing true breaks in DNA.
Parodi
S
S
Department of Clinical and Experimental Oncology, University of Genoa, Italy.
Abelmoschi
M L
ML
Balbi
C
C
De Angeli
M T
MT
Pala
M
M
Russo
P
P
Taningher
M
M
Santi
L
L
D000818:Animals; D001381:Azepines / Q000633:toxicity*; D001573:Benzoin / Q000633:toxicity*; D002209:Caprolactam / Q000633:toxicity*; D004249:DNA Damage; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D009153:Mutagens; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence
D001381:Azepines; D009153:Mutagens; D002209:Caprolactam; D001573:Benzoin