Effect of diclofenac on prostaglandin E and hyaluronic acid production by human synovial fibroblasts stimulated with interleukin-1.
Cultured human synovial fibroblasts were stimulated with human recombinant interleukin 1 beta to overproduce prostaglandin E (PGE) and hyaluronic acid (HA). Diclofenac, indomethacin and hydrocortisone inhibited stimulation of PGE production. Half-maximal inhibition occurred at 1.10 x 10(-9), 2.79 x 10(-8) and at 5.52 x 10(-8) M, respectively. Diclofenac or indomethacin had no effect on HA production, while hydrocortisone had an inhibitory effect (half-maximal inhibition at 2.76 x 10(-7) M). This model could be a useful in vitro indicator for the in vivo pharmacological actions of anti-inflammatory drugs.
Meyer
F A
FA
Department of Rheumatology, Ichilov Hospital, Tel Aviv, Israel.
Yaron
I
I
Mashiah
V
V
Yaron
M
M
D004008:Diclofenac / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D006854:Hydrocortisone / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D011458:Prostaglandins E / Q000096:biosynthesis*; D013268:Stimulation, Chemical; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*
D007375:Interleukin-1; D011458:Prostaglandins E; D004008:Diclofenac; D006820:Hyaluronic Acid; D006854:Hydrocortisone; D007213:Indomethacin