[Phenotype expression and production of IL-2 by tonsillar and blood lymphocytes in patients with tonsil pathology].
In recent years several studies have attempted to investigate immunological responses in different tonsillar pathologies, including recurrent tonsillitis and focal infections. The present study was performed on ten patients who had undergone tonsillectomy for a) simple hypertrophy, b) recurrent tonsillitis and c) recurrent tonsillitis with focal manifestations. The blood (PBMNC) and tonsillar (TMNC) lymphocytes were tested separately. A subsequent investigation was performed on the PBMNC six months after surgery. The phenotypical aspects of the different subpopulations were studied using fluorescent antiserums and monoclonal antibodies. A second field of investigation concerned the in vitro blastogenesis, which was measured under spontaneous and PHA-P induced conditions. Finally, the IL-2 production was evaluated using the induced-growth capacity of an IL-2 dependent clone of a T murine cell line. The most interesting findings are presented. The phenotypical studies confirmed some peculiar aspects of the representation of tonsillar subsets. According to the hyperactivation ratio, the proliferation data proved to be higher in the tonsil than in the blood. A greater number of positive HLA-DR and IL-2 receptor cells (both antibodies being activated cell markers) was seen in the tonsil than in the blood and this, as well as the sporadic presence of spontaneous blastogenesis, suggests the possibility of an in vivo pre-activated condition. As far as the secretion of IL-2 is concerned, when compared to the peripheral blood ratio, greater production was found in the tonsil. Finally, a different production kinetic appears to be a constant result of the present study. The performed tests were unable to demonstrate any particular differences among the three different groups of pathologies.
Bussi
M
M
Carlevato
M T
MT
Zoppo
M
M
Roncarolo
M G
MG
D000293:Adolescent; D002648:Child; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D007818:Laryngeal Diseases / Q000097:blood / Q000473:pathology; D008214:Lymphocytes / Q000145:classification* / Q000378:metabolism; D008297:Male; D014066:Palatine Tonsil / Q000378:metabolism; D010641:Phenotype
D007376:Interleukin-2
Espressione fenotipica e produzione di IL-2 da parte di linfociti tonsillari ed ematici in soggetti con patologia tonsillare.