Method of measuring liquid junction potentials in small (nanolitre) droplets of biological fluids.
1. A method is described which allows the determination of liquid junction potentials between biological fluid samples of less than 1 nL with a reproducibility of 10%. This was achieved by use of a micro-method and the use of 3% agarose added to the electrolyte in the measuring electrodes. 2. Validation was performed with a modified flowing boundary method and the system was also tested using a solution containing divalent ions. Liquid junction potential between surface fluid from rat kidneys and harvested renal proximal tubular fluids was -0.62 +/- 0.11 mV (mean +/- s.d., n = 66).
Sugo
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Department of Medicine, Sydney University, New South Wales, Australia.
Györy
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AZ
D001826:Body Fluids / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004566:Electrodes; D004573:Electrolytes / Q000032:analysis; D012834:Silver / Q000032:analysis; D018030:Silver Compounds
D004573:Electrolytes; D018030:Silver Compounds; D012834:Silver; C037548:silver chloride