Two peaks of interleukin 1 expression in human leukocytes cultured with tobacco glycoprotein.
We have previously shown that tobacco glycoprotein (TGP), a polyphenol-rich glycoprotein isolated from tobacco or from cigarette smoke, affects the immune system. In this study we show that TGP induces human PBL and adherent cells to produce IL-1 alpha and IL-1 beta. Two peaks of IL-1 activity were observed; one at 18-24 h, the second at 4-6 d after initiation of culture. A similar pattern was observed for the steady state level of IL-1 mRNA. These data suggest that the production of IL-1 by cells stimulated with TGP might be a factor in cardiovascular disease associated with cigarette smoking.
Francus
T
T
Division of Allergy and Immunology, Cornell University Medical College, New York, New York 10021.
Manzo
G
G
Canki
M
M
Thompson
L C
LC
Szabo
P
P
D000941:Antigens / Q000494:pharmacology*; D002478:Cells, Cultured; D006023:Glycoproteins; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000097:blood / Q000302:isolation & purification; D007700:Kinetics; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D008934:Mitogens / Q000494:pharmacology*; D010636:Phenols / Q000494:pharmacology*; D010940:Plant Proteins
D000941:Antigens; D006023:Glycoproteins; D007375:Interleukin-1; D008934:Mitogens; D010636:Phenols; D010940:Plant Proteins; C046039:TGP protein, Nicotiana tabacum