title: Identification of reaction products from the pyridinium dichromate derivatization of prostaglandins by high-performance liquid chromatography and direct chemical ionization mass spectrometry.
abstract: The reaction products from the oxidation of prostaglandins with pyridinium dichromate have been identified by direct chemical ionization mass spectrometry of the underivatized compounds after separation by reversed-phase high-performance liquid chromatography and ultraviolet diode-array detection. The thermal influence on the reproducibility of the dehydration patterns of the mass spectra was studied. The main products from the prostaglandins E1, E2, F1 alpha and F2 alpha were the corresponding 15-oxo derivatives. Minor amounts of the 9,11,15-trioxoprostaglandin were formed from PGE, while the oxidation of PGF was less selective, yielding additional dioxo derivatives. Addition of water to the reagent reduced the reactivity, but increased the selectivity in favour of the formation of 15-oxo-PGF during the oxidation of PGF.
authors: Department of Chemistry, University of Oslo, Norway.
authors: J
authors: J
authors: Doehl
authors: T
authors: T
authors: Greibrokk
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents; D013058:Mass Spectrometry / Q000379:methods; D010084:Oxidation-Reduction; D011453:Prostaglandins / Q000032:analysis*; D011726:Pyridinium Compounds
chemical_list: D007202:Indicators and Reagents; D011453:Prostaglandins; D011726:Pyridinium Compounds; C061685:pyridinium dichromate