Biochemical studies on the release of catecholamines from the rat carotid body in vitro.
The effects of hypoxia and carbachol on the release of newly synthesized catecholamines from superfused rat carotid bodies have been examined. Hypoxic superfusion medium was found to evoke catecholamine release which was dependent on the extracellular calcium concentration and was reduced by nitrendipine and atropine. Superfusion with the muscarinic agonist, carbachol, stimulated catecholamine release independently of the oxygen tension of the medium. The effect of carbachol on catecholamine release was abolished by atropine, suggesting that it was mediated by activation of cholinergic receptors of the muscarinic type. Both hypoxia and carbachol stimulated the release of 45Ca from carotid bodies prelabelled with 45Ca. The release of 45Ca with either stimulus was reduced by atropine and nitrendipine. These results suggest that although extracellular calcium plays an important role in the exocytotic secretory process of the carotid body, the mobilization of intracellular calcium pools may also contribute to the secretory response.
Shaw
K
K
Department of Biochemistry, University of Leicester School of Medicine, U.K.
Montague
W
W
Pallot
D J
DJ
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002344:Carotid Body / Q000187:drug effects / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D002852:Chromatography, Ion Exchange; D004298:Dopamine / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009568:Nitrendipine / Q000494:pharmacology; D009638:Norepinephrine / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D001285:Atropine; D002217:Carbachol; D009568:Nitrendipine; D002118:Calcium; D004298:Dopamine; D009638:Norepinephrine