Estradiol-stimulated increases in uterine eosinophils and nuclear type II estrogen-binding sites are prevented by pertussis toxin.
Previous studies from several laboratories have demonstrated that estradiol treatment resulted in an increase in nuclear type II binding sites. Our previous data suggest that this increase was due to the estradiol-stimulated influx of circulating eosinophils. Therefore, we suggested that the uterine nuclear type II estrogen-binding sites were not of uterine origin. In this report we present further evidence to support this hypothesis. Treatment of immature rats with estradiol resulted in the stimulation of several uterine parameters, namely wet weight, protein synthesis, eosinophil number, peroxidase activity, nuclear type II binding sites, and the synthesis and secretion of a 180-kDa protein. The coadministration of pertussigen had no effect on the estradiol-stimulated increase in wet weight, protein synthesis, or the synthesis and secretion of the 180-kDa protein. However, pertussigen did prevent the estradiol-stimulated increase in eosinophils, peroxidase activity, and nuclear type II binding sites, demonstrating a coordinated response. Since peroxidase activity is known to be contained int he eosinophil, these data are consistent with our earlier demonstration that the type II sites are of eosinophil origin. These data also support and extend our previous findings in neonatal animals that estradiol can stimulate a growth response without a corresponding increase in the nuclear type II binding sites. These results further indicate that the estradiol-stimulated increase in eosinophils does not appear to play a key role in the control of uterine growth.
Lyttle
C R
CR
Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104.
Teuscher
C
C
Medlock
K L
KL
Sheehan
D M
DM
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D010544:Peroxidases / Q000378:metabolism; D037342:Pertussis Toxin; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000187:drug effects / Q000502:physiology*; D012016:Reference Values; D014599:Uterus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D004965:Estrogen Antagonists; D011960:Receptors, Estrogen; D010566:Virulence Factors, Bordetella; D004958:Estradiol; D010544:Peroxidases; D037342:Pertussis Toxin