[Studies on the hepatitis B virus in cell culture: I. Expression of cloned HBV DNA in the HEP G2 cell line].
To study the interactions between HBV and host cells, cloned adr subtype HBV DNA dimer was used to transfect Hep G2 cell line. Five days after transfection, HBeAg could be detected in the supernatant and reached its peak on the 11th day. It was still positive on the 25th day. In contrast, HBsAg was only positive on the 8th day, and gradually increased until the 25th day when the amount of HBsAg was still rising. Supernatant of HBeAg positive samples was collected and after ultracentrifugation, was checked by electron microscopy. HBV like particles of 42 nm in diameter were detected. The prospect of employing this transient cell expression system for studies of HBV replication, inhibitory effects of drugs and immunological factors are discussed.
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D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D004279:DNA, Viral