title: Renal transplant patients monitored by the cell-mediated lympholysis assay. Evaluation of its clinical value.
abstract: Donor-specific cytotoxic T cell activity was measured over a period of 5 years after transplantation using the cell-mediated lympholysis (CML) test in 124 recipients of unrelated kidney allografts who received conventional immunosuppressive therapy consisting of azathioprine and prednisone. Since patients with a functioning transplant frequently display donor-specific CML non-responsiveness in vitro, we addressed the question of whether the CML status has a predictive value regarding the graft prognosis at any time interval until 5 years posttransplantation. From log-rank type analyses we conclude that the estimated relative risk calculated over the whole follow-up period of a CML-responder in the category of transplant rejectors is 1.25 with 95% confidence bounds between 0.94 and 1.65. Measurements of CML responder status during follow-up seem to have only limited prognostic value, although the relative risk is borderline significant when the analysis is restricted to the period between 2 weeks and 6 months posttransplantation.
authors: Department of Immunohaematology, University Hospital, Leiden, the Netherlands.
authors: E
authors: E
authors: Goulmy
authors: T
authors: T
authors: Stijnen
authors: A F
authors: AF
authors: Groenewoud
authors: G G
authors: GG
authors: Persijn
authors: E
authors: E
authors: Blokland
authors: J
authors: J
authors: Pool
authors: L C
authors: LC
authors: Paul
authors: J J
authors: JJ
authors: van Rood
mesh_terms: D003601:Cytotoxicity Tests, Immunologic; D006085:Graft Survival; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D011379:Prognosis