Solubilizing the T-cell receptor--problems in solution.
Recombinant DNA technology has been central in answering some of the most important questions in immunology and has recently helped to define the complex of membrane associated proteins on T-cell surfaces responsible for antigen recognition. Here André Traunecker and colleagues describe attempts to facilitate the analysis of this complex using genetic engineering to produce solubilized T-cell receptor and associated molecules.
Traunecker
A
A
Dolder
B
B
Oliveri
F
F
Karjalainen
K
K
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000302:isolation & purification; D003001:Cloning, Molecular / Q000379:methods*; D005813:Genes, Synthetic; D000949:Histocompatibility Antigens Class II / Q000302:isolation & purification; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification*; D051379:Mice; D011487:Protein Conformation; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012995:Solubility; D013601:T-Lymphocytes / Q000032:analysis
D000945:Antigens, Differentiation, T-Lymphocyte; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins