Porous-layer open-tubular gas chromatography in combination with an ion trap detector to assess volatile metabolites in human breath.
A method is described for the quantification of major volatile substances in human breath without preconcentration. Methanol, ethanol, acetaldehyde and acetone are well separated by porous-layer open-tubular column gas chromatography. Low-level detection was possible by means of an ion trap detector. Halothane has been used as internal standard.
Ghoos
Y
Y
Department of Medicine, UZ Gasthuisberg, Leuven, Belgium.
Hiele
M
M
Rutgeerts
P
P
Vantrappen
G
G
D000079:Acetaldehyde / Q000032:analysis; D000096:Acetone / Q000032:analysis; D001944:Breath Tests; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D000431:Ethanol / Q000032:analysis; D006221:Halothane / Q000032:analysis; D006801:Humans; D000432:Methanol / Q000032:analysis; D012015:Reference Standards
D000096:Acetone; D000431:Ethanol; D000079:Acetaldehyde; D006221:Halothane; D000432:Methanol