In vitro stimulation of nonimmunized, naturally infected rat spleen cells for the production of Mycoplasma pulmonis-specific monoclonal antibodies.
Spleen cells derived from rats naturally-infected with Mycoplasma pulmonis were stimulated in vitro, and then fused with a mouse myeloma cell line. The resulting hybridomas were screened for mycoplasma-specific Mabs by ELISA and for hemolysis-blocking activities. Fusions performed with in vitro-treated spleen cells yielded larger numbers of growth-positive wells and antibody secreting cells than untreated spleen cells from the same animals. Hybridomas derived from naturally-infected animals gave a higher percentage of hemolysin-specific monoclonal antibodies than did hyperimmunized animals. This indicated that B cell priming during mucosal infections can produce antigen-primed spleen cells. Stimulation of these cells in vitro can result in monoclonal antibodies against antigens not normally recognized during immunization with in vitro grown pathogenic bacteria.
Jarvill
K J
KJ
Veterinary Medical Research Institute, Iowa State University, Ames.
Minion
F C
FC
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology; D012376:Rodent Diseases / Q000276:immunology / Q000473:pathology*; D013154:Spleen / Q000276:immunology / Q000473:pathology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial