Allergen-specific induction of interleukin-2 (IL-2) responsiveness in lymphocytes from children with asthma. II. Regulation of IL-2 responsiveness by supernatants of normal lymphocytes.
Induction of interleukin-2 (IL-2) responsiveness by Dermatophagoides farinae (Df)-stimulated lymphocytes from children with asthma was markedly suppressed after the addition of culture supernatants from Df-stimulated normal T cells. Ovalbumin (OVA)-induced IL-2 responsiveness of lymphocytes from patients with hen-egg allergy was not suppressed by the supernatant from Df-stimulated T cells, indicating the antigenic specificity of the effect. Patients' lymphocytes whose IL-2 responsiveness was decreased still expressed Tac antigen (low-affinity IL-2 receptors) but, in contrast to the patients' original lymphocytes, did not absorb or respond to IL-2, suggesting the loss of high-affinity IL-2 receptors (p55/p75) from these cells. The supernatant from Df-stimulated normal T cells from one individual did not necessarily suppress the response of all patients tested, indicating the existence of some allogeneic barrier between the factor(s) and patients' lymphocytes.
Noma
T
T
Department of Pediatrics, Saitama Medical School, Japan.
Yoshizawa
I
I
Maeda
K
K
Ichikawa
K
K
Baba
M
M
Yata
J
J
D000042:Absorption; D000485:Allergens / Q000276:immunology*; D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects; D007376:Interleukin-2 / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008925:Mites / Q000276:immunology; D010047:Ovalbumin / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D000485:Allergens; D007376:Interleukin-2; D011994:Recombinant Proteins; D010047:Ovalbumin