Analysis of drug in tissue homogenates by high performance liquid chromatography with direct injection and column switching.
An HPLC method with direct sample injections and column switching was investigated for the analysis of drugs in tissue homogenates. The appropriate precolumn packings and analytical column packings were surveyed in order to obtain quantitative recovery. The use of a large bore end-fitting filter for the precolumn avoided the interference due to minute tissue particles. A minicolumn was used to trap the analyte or clean up the sample. The method was applicable to hydrophilic as well as hydrophobic drugs in liver, kidney or heart tissues, and has been extensively used in the determination of drugs in centrifugal cell fractions such as the nucleus, mitochondria and microsome.
Imai
H
H
Faculty of Pharmaceutical Sciences, Fukuyama University, Japan.
Tamai
G
G
D000818:Animals; D002474:Cell-Free System; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005472:Fluorouracil / Q000032:analysis; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008727:Methotrexate / Q000032:analysis; D051379:Mice; D009206:Myocardium / Q000032:analysis; D015388:Organelles / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011342:Procainamide / Q000032:analysis; D011433:Propranolol / Q000032:analysis
D004364:Pharmaceutical Preparations; D011433:Propranolol; D011342:Procainamide; D005472:Fluorouracil; D008727:Methotrexate