Crystallization and preliminary X-ray investigation of a complex between a Fab fragment and its antigen, cyclosporin.
Preliminary crystallographic data are given for a complex between the cyclic undecapeptide cyclosporin and the Fab fragment of an anti-cyclosporin monoclonal antibody. Crystals of the complex are orthorhombic with space group P2(1)2(1)2(1) and diffract to 2.7 A resolution. The unit cell dimensions are a = 52.6 A, b = 70.2 A and c = 118.4 A. A native data set to 2.7 A resolution has been collected.
Altschuh
D
D
Laboratoire d'Immunochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
Kocher
H P
HP
Quesniaux
V F
VF
Schmitter
D
D
Van Regenmortel
M H
MH
Thierry
J C
JC
D000911:Antibodies, Monoclonal; D003460:Crystallization; D003524:Cyclosporins; D007140:Immunoglobulin Fab Fragments; D046911:Macromolecular Substances; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D003524:Cyclosporins; D007140:Immunoglobulin Fab Fragments; D046911:Macromolecular Substances