Development of specific human mab's by a small scale electrofusion technique: the influence of some physical and chemical factors on hybridoma yield of human peripheral blood lymphocytes XCB-F7 fusions.
A fusion chamber and an appropriate procedure are described which allow to fuse a sample of 15 to 25 microliters of cell suspension every two minutes. The cells can be observed throughout the process. They are not exposed to mechanical stress after the fusion pulse. Electrofusion between the heteromyeloma line CB-F7 and human mononuclear cells from peripheral blood of immunized donors is shown to provide stable hybridomas producing IgG against tetanustoxin. Pronase treatment, calmodulin, PEG, lanthanum and a number of variations in the fusion conditions were investigated as to whether they influence physical fusion of the cells, hybridoma yield, and immunoglobulin production.
Glaser
R W
RW
Department of Medicine (Charité), Humboldt-University Berlin.
Jahn
S
S
Grunow
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002459:Cell Fusion / Q000295:instrumentation*; D002460:Cell Line; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology
D000911:Antibodies, Monoclonal; D013745:Tetanus Toxoid