title: Presentation of antigen to T lymphocytes by non-immune B-cell hybridoma clones: evidence for specific and non-specific presentation.
abstract: Non-immune SJL (H-2s) spleen cells were fused with non-secreting, non-antigen presenting (H-2d) Balb/c 653-myeloma cells and the hybridomas were cloned by two limiting dilutions. The resulting hybrid B-cell clones were tested for their antigen presentation capability to SJL T-cell lines that were specific for either lysozyme or myoglobin. In proliferative assays, 53% of the antigen presenting B-cell clones presented both myoglobin and lysozyme (general presenters) while the other 47% presented specifically either myoglobin or lysozyme (specific presenters). The ability to selectively present either myoglobin or lysozyme indicates that antigen presentation at the clonal level can be specific or non-specific depending on the particular B-cell clone.
authors: NASA-Johnson Space Center, Houston, Texas 77058.
authors: H H
authors: HH
authors: Cohly
authors: D R
authors: DR
authors: Morrison
authors: M Z
authors: MZ
authors: Atassi
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D006825:Hybridomas / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009113:Muramidase / Q000378:metabolism; D009211:Myoglobin / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D009211:Myoglobin; D009113:Muramidase