Autoantibodies to the major nucleolar phosphoprotein B23 define a novel subset of patients with anticardiolipin antibodies.
Of 164 sera with antinucleolar antibodies, 7 (4.3%) were shown by Western blotting to react with a 37-kd polypeptide in a nuclear extract of HeLa cells and with the recombinant protein expressed by a complementary DNA clone encoding the major nucleolar protein B23. Six of the 7 sera (86%) had antibodies to cardiolipin (aCL), and the sample that was negative for aCL had had lupus anticoagulant on previous testing. All 7 patients had either systemic lupus erythematosus (SLE) or a variant of SLE, suggesting that anti-B23 identifies a subset of patients with SLE associated with a high frequency of aCL.
Li
X Z
XZ
Burnet Clinical Research Unit, Walter & Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
McNeilage
L J
LJ
Whittingham
S
S
D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000032:analysis*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000276:immunology; D002308:Cardiolipins / Q000276:immunology*; D002466:Cell Nucleolus / Q000276:immunology*; D004247:DNA / Q000276:immunology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000276:immunology*; D000090243:Nucleophosmin; D011994:Recombinant Proteins / Q000276:immunology; D012216:Rheumatic Diseases / Q000276:immunology
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D002308:Cardiolipins; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D000090243:Nucleophosmin; D004247:DNA