Effect of in vitro ultraviolet radiation on the binding capacity of anti-DNA and DNA in systemic lupus erythematosus.
The in vitro effects of various doses of ultra violet A (UVA) or UVB irradiation on DNA or anti-DNA as measured by their subsequent binding to anti-DNA or DNA were examined. Sera from 12 patients with active lupus were studied. UVA irradiation at 20-240 J/m2 or UVB irradiation at 2-24 J/m2 did not affect DNA or anti-DNA binding to anti-DNA or DNA, respectively. Modulation of the UV effect on DNA and anti-DNA by adding methylprednisolone, hydroxychloroquine and indomethacin at 10(-6) M did not alter the UV effect. UV light does not exert its effect directly on the binding of anti-DNA to DNA.
Becker
N J
NJ
Department of Medicine, University of Kansas, School of Medicine, Kansas City 66103.
Crockett
R S
RS
Valenzeno
D P
DP
Abdou
N I
NI
D000974:Antibodies, Antinuclear / Q000276:immunology; D000915:Antibody Affinity / Q000528:radiation effects; D002869:Chromosome Aberrations / Q000528:radiation effects; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006886:Hydroxychloroquine / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008775:Methylprednisolone / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000974:Antibodies, Antinuclear; D006886:Hydroxychloroquine; D004247:DNA; D008775:Methylprednisolone; D007213:Indomethacin