[Cloning genes, induced by x-ray irradiation in rat liver cells].
The library of cDNAs synthesized on poly(A)+ mRNA derived from rat liver after total X-irradiation of animals has been obtained. cDNA-clones (p gamma clones) representing sequences inducibly transcribed as a result of radiation treatment were isolated by differential screening. The increased expression of p gamma mRNAs was observed during the period from 6 to 24 h after irradiation by 3-6 Gy dose. It was concluded that p gamma mRNAs do not code for the liver-specified proteins because these mRNAs are also present in the rat fibroblasts. We suggest that p gamma genes are involved in the pathways of DNA-repair system.
Timchenko
L T
LT
Khrustovskaia
O I
OI
Noskin
L A
LA
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics*; D015870:Gene Expression; D015698:Genomic Library; D008099:Liver / Q000166:cytology / Q000528:radiation effects*; D008115:Liver Regeneration; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D051381:Rats; D014158:Transcription, Genetic
D004247:DNA
Klonirovanie genov, indutsiruemykh rentgenovskim oblucheniem v kletkakh pecheni krysy.