Seasonal variation in hepatic binding of estrogen in the turtle, Chrysemys picta.
The present study describes the seasonal changes of the estrogen receptor (ER) system in the liver of the turtle, Chrysemys picta. [3H]Estradiol ([3H]E2) binding capacities and affinities of liver cytosols and nuclear extracts were measured with established procedures and analyzed by Scatchard plots. Our data revealed significant seasonal variations in both receptor content and the ER's affinity for [3H]E2 in the liver of the turtle. Nuclear ER content remained at a fairly stable level of 70 fmol/g tissue throughout the year, but exhibited two sharp increases in the months of May and October, attaining values of approximately 150 fmol/g tissue. These rises in nuclear ER content coincided temporally with the two peaks of vitellogenin accumulation and estrogen surge in the annual cycle previously reported for the female turtle. Cytosolic ER level in the liver exhibited a much more complex pattern. It fluctuated with a much wider range of 80 to 250 fmol/g tissue. Right after oviposition in July, it declined to its lowest value of 80 +/- 9 fmol/g tissue but gradually increased to 160 +/- 15 fmol/g tissue in September and remained at this medium range for the remaining of the fall season but rose sharply again to the highest value of the year in December, reaching a value of 270 +/- 7 fmol/g tissue. In the following winter months, cytosolic ER level declined until March before it rebounded to a second peak value of 242 +/- 6 fmol/g tissue in May.(ABSTRACT TRUNCATED AT 250 WORDS)
Yu
M S
MS
Department of Biology, Tufts University, Medford, Massachusetts 02155.
Ho
S M
SM
D000818:Animals; D005260:Female; D008099:Liver / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D012621:Seasons; D014426:Turtles / Q000378:metabolism*
D011960:Receptors, Estrogen