Increase in Tac mRNA expression in natural killer-like cell line in response to interleukin-1 and interleukin-2 shown at the population and cellular level by in situ hybridization.
Our previous results have shown that the increased expression of the Tac component of the interleukin-2 (IL-2) receptor at the surface of a natural killer-like cell line (YTN10) in response to interleukin-1 (IL-1) is associated with an eightfold increase in Tac mRNA production. Tac expression was detected by flow cytometry and anti-Tac binding studies, and mRNA synthesis was assessed by Northern and dot blots. These findings have now been extended utilizing recombinant IL-1 and autoradiographic techniques in experiments using both the parent YT and YTN10 cells. In situ hybridization experiments have shown that the increase in mRNA expression is manifested both in the percentage of cells expressing it and in the level of expression per cell. Consistent results were obtained in both sublines. In addition, exposure of both sublines to recombinant IL-2 also induced increased Tac mRNA synthesis, while inducing only a marginal increase in Tac expression at the membrane level.
Fong
T C
TC
Geriatric Research, Education and Clinical Center (GRECC), Veterans Administration Medical Center, West Los Angeles, Calif.
Babbitt
J T
JT
Bloom
E T
ET
D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000166:cytology / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D015375:Receptors, Interleukin-2 / Q000235:genetics*; D011994:Recombinant Proteins
D007375:Interleukin-1; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins