title: Effect of starvation and sampling time on plasma alkaline phosphatase activity and calcium homeostasis in the rat.
abstract: The effect of starvation and sampling time on plasma alkaline phosphatase activity, total plasma calcium concentration and whole blood ionized calcium concentration was determined in the rat. Starvation caused a significant fall in total and ionized calcium concentrations as well as in alkaline phosphatase activity. These changes were accompanied by a fall in whole blood pH and an increase in the anion gap and a decrease in urinary excretion of calcium. These indices were restored to normal following refeeding. There was no change in serum 25-OH vitamin D concentrations following starvation for 3 days. Alkaline phosphatase activity showed a pattern compatible with the presence of a circadian rhythm when sampling took place between 0800 and 1800 h. Total and ionized calcium concentrations did not show such a rhythm when animals were fed the present diet.
authors: Department of Chemical Pathology and Human Metabolism, Royal Free Hospital and School of Medicine, London, UK.
authors: C S
authors: CS
authors: Thompson
authors: D P
authors: DP
authors: Mikhailidis
authors: D S
authors: DS
authors: Gill
authors: J Y
authors: JY
authors: Jeremy
authors: J L
authors: JL
authors: Bell
authors: P
authors: P
authors: Dandona
mesh_terms: D015652:25-Hydroxyvitamin D 2; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001835:Body Weight / Q000662:veterinary; D002118:Calcium / Q000097:blood* / Q000652:urine; D002940:Circadian Rhythm; D004872:Ergocalciferols / Q000031:analogs & derivatives / Q000097:blood; D006706:Homeostasis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains / Q000097:blood*; D013217:Starvation / Q000662:veterinary*; D013997:Time Factors
chemical_list: D004872:Ergocalciferols; D015652:25-Hydroxyvitamin D 2; D000469:Alkaline Phosphatase; D002118:Calcium