v-src genes stimulate neurite outgrowth in pheochromocytoma (PC12) variants unresponsive to neurotrophic factors.
The NGF-nonresponsive rat pheochromocytoma PC12 variant nnr5, isolated by Green et al. (J Cell Biol 102:830-843, 1986), responds poorly or not at all to fibroblast growth factor. Transformation of PC-12nnr5 cells with v-src-expressing retroviruses results in vigorous neurite outgrowth, similar to that seen in the parent cell line. Thus though the PC12nnr5 cell line has a greatly impaired ability to respond to neurotrophic factors it still may extend neurites. This data is consistent with a model in which PC12nnr5 cells are unable to propagate intracellular second messengers, and this defect may be related to the expression of c-src gene products.
Eveleth
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DD
Department of Biological Chemistry, California College of Medicine, Irvine.
Hanecak
R
R
Fox
G M
GM
Fan
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Bradshaw
R A
RA
D000818:Animals; D001369:Axons / Q000502:physiology*; D003001:Cloning, Molecular; D009414:Nerve Growth Factors / Q000502:physiology*; D009857:Oncogenes / Q000502:physiology*; D010673:Pheochromocytoma; D051381:Rats; D012190:Retroviridae / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000502:physiology
D009414:Nerve Growth Factors