Total reconstitution of Salmonella flagellar filaments from hook and purified flagellin and hook-associated proteins in vitro.
Salmonella flagellar filaments comprise the following distinct parts connected in series: a curved hook composed of a single kind of subunit (hook protein); two short segments made up of hook-associated proteins (HAP1 and HAP3); a long helical filament composed of flagellin; and a cap composed of HAP2. In this study, a procedure was developed to isolate HAPs from the culture medium of a short-flagella mutant. We demonstrate that hook-filament complex can be formed in vitro by sequential addition of HAP1, HAP3 and flagellin to hook fragments.
Ikeda
T
T
Exploratory Research for Advanced Technology, Research Development Corporation of Japan, Kyoto.
Asakura
S
S
Kamiya
R
R
D001426:Bacterial Proteins / Q000302:isolation & purification*; D005407:Flagella / Q000648:ultrastructure*; D005408:Flagellin / Q000302:isolation & purification*; D008854:Microscopy, Electron; D009154:Mutation; D012475:Salmonella / Q000235:genetics / Q000648:ultrastructure*
D001426:Bacterial Proteins; C035832:hook protein, bacterial flagellum; D005408:Flagellin