Effect of magnesium deficiency on interleukin production by Fisher rats: effect of interleukins on reduced in vitro lymphocyte responses to concanavalin A and lipopolysaccharide.
The ability of rats fed a magnesium-deficient diet to produce interleukins (ILs) and the effect of ILs on in vitro lymphocyte mitogenesis have been studied in rats. Lack of magnesium resulted in a lower number of plastic-adherent spleen cells and in a reduction of IL-1 production. IL-2 production was not significantly affected, indicating differential sensitivity of T cells to magnesium deficiency. The diminished mitogenic response of splenocytes to concanavalin A (Con-A) was restored by the addition of IL-1 supernatant, while the addition of IL-2 supernatant and recombinant IL-2 resulted in significantly greater enhancement of proliferation in response to Con-A, compared with that of control spleen cells. The fact that recombinant IL-2 restored the T-cell responses to Con-A indicates that the active factor in the IL-2 supernatant is IL-2.
Yiangou
M
M
Department of Biology, School of Science, Aristotelian University of Thessaloniki, Greece.
Hadjipetrou-Kourounakis
L
L
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000378:metabolism*; D008275:Magnesium Deficiency / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000378:metabolism*
D007375:Interleukin-1; D007376:Interleukin-2; D008070:Lipopolysaccharides; D003208:Concanavalin A