RepB' is required in trans for the two single-strand DNA initiation signals in oriV of plasmid RSF1010.
We have shown previously [Honda et al., Gene 68 (1988) 221-228] that the oriV region of the broad-host-range plasmid RSF1010 contained two single-strand DNA initiation signals (ssi) which have RSF1010-specific properties since they required one or more factors provided in trans by RSF1010 for their functional activities. We demonstrate here, by deletion analysis, that repB', one of the genes essential for the vegetative replication of RSF1010, produces a factor required for the function of ssi signals. It is conceivable that the RepB' protein and the two ssi signals, ssiA and ssiB, cooperatively compose plasmid-specific priming complexes which confer the broad-host-range property upon RSF1010.
Honda
Y
Y
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University, Japan.
Sakai
H
H
Komano
T
T
Bagdasarian
M
M
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001435:Bacteriophages / Q000235:genetics; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; C048817:RepB protein, Bacteria