Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs.
Three hamster monoclonal antibodies (mAbs), all recognizing different epitopes present on the native form of the murine T-cell antigen receptor (TCR) gamma delta subunits, have been generated. mAb 3A10 is specific to a pan-murine TCR gamma delta, recognizing a C delta constant region determinant. mAb 8D6 is specific to a subset of T cells expressing V gamma 4- and V delta 5-encoded gamma delta TCR, and mAb 5C10 is clonotypic. Using these and other mAbs directed against a variety of T-cell surface markers, we quantitated and characterized gamma delta T cells present in developing thymuses as well as in the conventional lymphatic organs by flow cytometry. These studies revealed that (i) many gamma delta thymocytes and peripheral T cells bear CD4 and/or CD8 molecules, (ii) T cells bearing both alpha beta and gamma delta TCRs are scarce, and (iii) thymocyte subsets bearing TCR gamma delta encoded by different combinations of V gamma and V delta gene segments appear in waves during ontogeny.
Itohara
S
S
Howard Hughes Medical Institute, Cambridge, MA 02139.
Nakanishi
N
N
Kanagawa
O
O
Kubo
R
R
Tonegawa
S
S
D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D006224:Cricetinae; D005865:Gestational Age; D008208:Lymphatic System / Q000196:embryology / Q000254:growth & development* / Q000276:immunology; D046911:Macromolecular Substances; D051379:Mice; D008815:Mice, Inbred Strains; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000196:embryology / Q000254:growth & development* / Q000276:immunology
D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell