Isolation of a cDNA encoding the rat liver S-adenosylmethionine synthetase.
We have isolated cDNA clones encoding the rat liver S-adenosylmethionine synthetase by means of immunological screening from a phage lambda gt 11 expression library containing cDNA synthesized from adult rat liver poly(A)-RNA. The amino acid sequence deduced from the cDNA indicates that the rat liver enzyme for this protein contains 397 amino acid residues and has a molecular mass of 43697 Da. The deduced amino acid sequence of rat liver S-adenosylmethionine synthetase was 68% similar to those of yeast S-adenosylmethionine synthetases encoded by two unlinked genes SAM1 and SAM2. The rat liver S-adenosylmethionine synthetase also shows 52% similarity with the deduced amino acid sequence of the MetK gene encoding the S-adenosylmethionine synthetase in Escherichia coli.
Horikawa
S
S
Department of Pathological Biochemistry, Tokyo Medical and Dental University, Japan.
Ishikawa
M
M
Ozasa
H
H
Tsukada
K
K
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D008716:Methionine Adenosyltransferase / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014166:Transferases / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA; D014166:Transferases; D008716:Methionine Adenosyltransferase