IL-3 induces differentiation of bone marrow precursor cells to osteoclast-like cells.
IL-3, a cytokine with hematopoietic differentiating capability, induced murine bone marrow cells to differentiate into cells resembling osteoclasts. The cells resulting from treatment with IL-3 were multi-nucleated and demonstrated tartrate-resistant acid-phosphatase activity, as do resident osteoclasts found in bone. IL-3-induced osteoclast-like cell development in the absence of serum-derived vitamin D metabolites, and a mAb that inhibited IL-3-induced proliferation of an addicted cell line also inhibited the development of osteoclasts in the presence of IL-3. The same Ab had no effect on 1 alpha, 25-dihydroxyvitamin D3-induced differentiation of osteoclasts. This newly described function of IL-3 may indicate a role for activated T cells in the bone resorption seen with rheumatoid arthritis.
Barton
B E
BE
Department of Allergy and Inflammation, Schering-Plough Research, Bloomfield, NJ 07003.
Mayer
R
R
D000135:Acid Phosphatase; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001853:Bone Marrow / Q000201:enzymology / Q000276:immunology / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006131:Growth Inhibitors / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000276:immunology / Q000502:physiology*; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007377:Interleukin-3 / Q000276:immunology / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010010:Osteoclasts / Q000201:enzymology / Q000276:immunology / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013644:Tartrates
D000911:Antibodies, Monoclonal; D006131:Growth Inhibitors; D007375:Interleukin-1; D007376:Interleukin-2; D007377:Interleukin-3; D011994:Recombinant Proteins; D013644:Tartrates; D000135:Acid Phosphatase