Molecular cloning of cDNA for sarcocystatin A and analysis of the expression of the sarcocystatin A gene during development of Sarcophaga peregrina.
Sarcocystatin A is a cysteine proteinase inhibitor purified from the hemolymph of Sarcophaga peregrina larvae [Suzuki, T., & Natori, S. (1985) J. Biol. Chem. 260, 5115-5120]. We isolated a cDNA clone for sarcocystatin A and analyzed the structure and expression of the sarcocystatin A gene. Sarcocystatin A consists of 102 amino acid residues. Significant homology was found between amino acid sequences of sarcocystatin A and other mammalian cystatins, and highly conserved sequences among mammalian cystatins were also found in sarcocystatin A. Using cloned cDNA as a probe, we investigated expression of the sarcocystatin A gene during the development of Sarcophaga. Results showed that this gene was transiently activated in the very early embryonic stage and in the pupal stage, suggesting that sarcocystatin A participates in morphogenesis of larval and adult structures of Sarcophaga.
Saito
H
H
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Suzuki
T
T
Ueno
K
K
Kubo
T
T
Natori
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015853:Cysteine Proteinase Inhibitors; D004247:DNA / Q000235:genetics; D004175:Diptera / Q000235:genetics* / Q000254:growth & development; D005796:Genes; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments; D011480:Protease Inhibitors / Q000235:genetics*; D011487:Protein Conformation; D015183:Restriction Mapping; D014357:Trypsin
D015853:Cysteine Proteinase Inhibitors; D010446:Peptide Fragments; D011480:Protease Inhibitors; D004247:DNA; D014357:Trypsin