The Mr 115 kd fiber cell-specific protein is a component of the lens cytoskeleton.
Electron microscope level immunocytochemistry was used to localize a lens fiber cell-specific protein with an Mr of 115 kd. Affinity-purified polyclonal antibodies were utilized on sections of detergent-extracted, acrylic-embedded lens cortical fiber cells. Monoclonal antibodies were utilized for pre-embeddment labelling of a subcellular fraction of lens fiber cells generated by homogenization, and high-speed centrifugation. The results indicate that the Mr 115 kd antigen is a component of the lens fiber cell cytoskeleton, specifically the beaded filament (BF), a cytoskeletal element thought to be unique to the differentiated lens fiber cell.
FitzGerald
P G
PG
Department of Human Anatomy, School of Medicine, University of California, Davis 95616.
Gottlieb
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002417:Cattle; D003598:Cytoskeletal Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis; D005404:Fixatives; D007908:Lens, Crystalline / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D008970:Molecular Weight; D013347:Subcellular Fractions
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D005136:Eye Proteins; D005404:Fixatives