title: Improved stability of 2,3-bisphosphoglycerate during storage of hexokinase-overloaded erythrocytes.
abstract: Human red blood cells were overloaded with homogeneous human hexokinase using a procedure of encapsulation based on hypotonic hemolysis and isotonic resealing and reannealing to achieve a final activity that was 15 times higher than that in control cells. Storage for 5 weeks at 4 degrees C of hexokinase-overloaded erythrocytes shows that these cells undergo small K+ leakage and mean cell volume increase compared with control cells. Furthermore, after these 5 weeks of storage the 2,3-bisphosphoglycerate content was normal while the ATP concentration was slightly reduced. These results and other properties suggest that encapsulation of key glycolytic enzymes in erythrocytes can provide a new way to maintain in vitro functionally active red blood cells for at least 5 weeks.
authors: Instituto di Chimica Biologica, Università degli Studi, Urbino, Italy.
authors: M
authors: M
authors: Magnani
authors: L
authors: L
authors: Rossi
authors: M
authors: M
authors: Bianchi
authors: G
authors: G
authors: Serafini
authors: E
authors: E
authors: Zocchi
authors: M
authors: M
authors: Laguerre
authors: C
authors: C
authors: Ropars
mesh_terms: D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D006593:Hexokinase / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate; D006593:Hexokinase