Activation of protein kinase C by an aromatic xenobiotic diacylglycerol analogue.
A number of xenobiotic carboxylic acids, including 3-phenoxybenzoic acid (3PBA), have been shown to form 'hybrid' di- and tri-acylglycerols both in vivo and in vitro. Experiments were carried out to test the hypothesis that naturally occurring xenobiotic diacylglycerols may stimulate protein kinase C. The activity of protein kinase C was measured in the presence of different diacylglycerols. 1-Acyl-2-(3PBA)-sn-glycerol but not 1,2-di-3PBA-sn-glycerol stimulated protein kinase C, but less effectively than either dioleoylglycerol or phorbol 12-myristate 13-acetate. The xenobiotic diacylglycerols were also more resistant to lipolysis. The possibility exists therefore that some xenobiotic diacylglycerols may behave, like phorbol diesters, as tumour promoters.
Moorhouse
K G
KG
Department of Biochemistry and Biological Sciences, Wye College, University of London, Ashford, Kent, England.
Hutson
D H
DH
Dodds
P F
PF
D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D001921:Brain / Q000201:enzymology; D002273:Carcinogens; D004075:Diglycerides / Q000378:metabolism / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005989:Glycerides / Q000494:pharmacology*; D008066:Lipolysis; D008297:Male; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015262:Xenobiotics / Q000494:pharmacology
D001565:Benzoates; D002273:Carcinogens; D004075:Diglycerides; D005989:Glycerides; D010703:Phorbol Esters; D015262:Xenobiotics; C017618:3-phenoxybenzoic acid; D011493:Protein Kinase C