Evidence for a novel growth factor in Xenopus oocytes.
Xenopus oocytes contain a novel growth factor, as determined by its effect on the anchorage-dependent and -independent growth of various rat cells and on a Xenopus cell line; it is destroyed by trypsin, acidification (pH 2.0), heating (100 degrees C, 3 min), 8 M urea but not by dithiothreitol. Gel filtration of this activity in nondissociating conditions suggests the existence of aggregates and the presence of a very high (approximately 2000 Kd) and a much lower (approximately 30 Kd) form.
Théry
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Unité 532 du CNRS, Institut Curie, Orsay, France.
Jullien
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Lawrence
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DA
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002457:Cell Extracts / Q000502:physiology; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006133:Growth Substances / Q000302:isolation & purification / Q000502:physiology*; D008970:Molecular Weight; D009865:Oocytes / Q000032:analysis*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051381:Rats; D015291:Transforming Growth Factors / Q000494:pharmacology; D014981:Xenopus
D002457:Cell Extracts; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors