[Antitrypsin activity of the urine and alpha 1 protease inhibitor].
The urinary trypsin inhibitory capacity (TIC) is mainly due to the excretion of an inhibitor which is immunologically related to inter-alpha:trypsin inhibitor (ITI). However, alpha 1 protease inhibitor (alpha 1 PI) can be found in urine of patients with proteinuria. When this one is greater than 1 g/l, the measured TIC is more or less markedly related to the amount of alpha 1 PI present in the analyzed sample. The antitryptic activity of alpha 1 PI can be ruled out by incubating urine with specific anti-alpha 1 PI immunoglobulins. An identical result is obtained by acidification of the sample prior to TIC determination. Moreover, after freezing of urine, only the antitryptic activity of alpha 1 PI is strikingly decreased. Thus, in the presence of a significant proteinuria (greater than 1 g/l), a preliminary acidification of urine allows a suitable and specific measurement of TIC due to the inhibitor immunologically related to ITI. Thus, this one is a sensitive, useful and easy test for detecting and monitoring infections.
Loutfti
H
H
Laboratoire de Biochimie, Faculté de Pharmacie, Lille.
Mizon
C
C
Balduyck
M
M
Gosset
D
D
Tacquet
A
A
Mizon
J
J
D001424:Bacterial Infections / Q000652:urine; D015415:Biomarkers / Q000652:urine; D006801:Humans; D011507:Proteinuria / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000652:urine*; D000515:alpha 1-Antitrypsin / Q000652:urine*
D015415:Biomarkers; D014361:Trypsin Inhibitors; D000515:alpha 1-Antitrypsin
Activité antitrypsique de l'urine et alpha 1 protéase inhibiteur.