[The surface phenotype and enzymatic cytochemical markers of B cells during pokeweed mitogen activation].
Methods of monoclonal antibodies, cytochemistry and rosette formation have been used to study antigens, receptors and enzymatic activity of peripheral blood lymphocytes during pokeweed mitogen-stimulation. After 18-20 hours in mitogen-stimulated cultures there is a decrease in number of T-lymphocytes that express CD7, CD5, CD4, CD8 antigens and of E-receptors and cells with the local activities of acid phosphatase and acid non-specific esterase. The number of lymphocytes with E37-, FcM- and M-receptors and of cells with granular PAS-reaction increased. Blast cells were revealed after 40-48 hours. Approximately 50% blast cells forming E37-rosettes and expressing CD7, CD5, CD2 antigens were characterized by the local activities of the above enzymes. The blasts did not express FcG-, FcM-, C3- or M-receptors. Cells like those at the Hodgkin disease and the "hand mirror" type cells were established on days 3-4. The number of lymphocytes with plasmatization was seen to increase by day 7.
Evsev'eva
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AI
Abramenko
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IV
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D002478:Cells, Cultured; D006651:Histocytochemistry; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D010641:Phenotype; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis / Q000276:immunology; D012397:Rosette Formation; D013499:Surface Properties; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D011043:Pokeweed Mitogens; D011947:Receptors, Antigen, B-Cell
Poverkhnostnyĭ fenotip i énzimotsitokhimicheskie markery B-kletok pri aktivatsii mitogenom lakonosa.