Adherence assay of 51chromium-labeled human polymorphonuclear leukocyte using glass beads.
There is a need for a reliable and relatively simple assay of in vitro adherence capacity. We have modified a previously described method of adherence of 51Cr-labeled PMN to glass beads and developed an assay which should be of value in both the clinical and research areas. The assay measures PMN adherence in the non-stimulated state and also following stimulation with phorbol myristate acetate (PMA) or endotoxin-activated plasma (EAP) using as few as 0.25 X 10(6) PMN. The differences observed between the adherence of stimulated and non-stimulated PMN were highly significant.
Kinoshita
Y
Y
Department of Pediatrics, University of California, Davis 95616.
Miller
M E
ME
Wuest
C J
CJ
D002448:Cell Adhesion; D002860:Chromium Radioisotopes; D004731:Endotoxins / Q000494:pharmacology; D005898:Glass; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002860:Chromium Radioisotopes; D004731:Endotoxins; D013755:Tetradecanoylphorbol Acetate