Experimental otitis media in mice. Immunohistochemical observations.
In order to identify T cells and B cells in the middle ear (ME) mucosa, immunohistochemical observations were performed on mice which were induced with immune-mediated otitis media with effusion (OME). C3H/HeN mice were challenged with dinitrophenylated ovalbumin in the ME, 2 weeks after the systemic immunization. At 1, 3 and 7 days post challenge, the mice were sacrificed, and IgG-, IgA- or IgM-positive cells, as well as Lyt-1- or Lyt-2-positive cells, were observed in the subepithelial layer of ME. IgG- or IgA-positive lymphocytes were detected at 7 days post challenge, whereas IgM-positive cells were rarely seen. At 3 days post challenge, the number of Lyt-1-positive lymphocytes exceeded that of Lyt-2-positive lymphocytes; however, at 7 days post challenge they were similar. Previous studies have suggested that immune complexes are a key factor in the generation of immune-mediated OME. However, it seems possible that delayed type hypersensitivity may contribute to the pathogenic mechanism of OME.
Ichimiya
I
I
Department of Otolaryngology, Medical College of Oita, Japan.
Ueyama
S
S
Mogi
G
G
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D004432:Ear, Middle / Q000473:pathology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010034:Otitis Media with Effusion / Q000378:metabolism* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*