Isolation of the complementary DNA for human transcobalamin II.
A complementary DNA (cDNA) clone coding for transcobalamin II (TCII) has been isolated from a human umbilical vein endothelial cell cDNA library. The cDNA is 1.9 Kb and includes the nucleotide sequence which encodes the NH2-terminal 19 amino acids of human TCII. The size of the cDNA is sufficient to code for the entire protein and also contains the nucleotide sequence coding for a 24 amino acid leader peptide and a long untranslated 3' region. The availability of this cDNA will provide the opportunity to characterize genetic disorders of TCII.
Platica
O
O
Department of Medicine, SUNY-Health Science Center, Brooklyn.
Geneczko
R
R
Regec
A
A
Quadros
E V
EV
Rothenberg
S P
SP
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D004730:Endothelium, Vascular / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014155:Transcobalamins / Q000235:genetics*; D014471:Umbilical Veins / Q000032:analysis
D014155:Transcobalamins; D004247:DNA