[Assessment of the functional activity of lymphokine-activated killer cells in healthy donors and in patients with interleukin-2-dependent immunodeficiency].
The methodological aspects of Lak-cells functional activity test were determined. NK-resistance of P-815 culture cells and NK-sensitive K-562 cells were used as target cells. The highest cytotoxicity level was registered at the 5-th day of culturing MNC with rIL-2 at the final concentration (100 U/ml). The method developed was used for testing healthy donors group and group of patients with IL-2 dependent immunodeficiency (ophthalmic herpes). Data obtained show the significant decrease of LAK-cells functional activity of patients in comparison with healthy donors. Thus, the developed method is recommended for complex estimation of immune status of patients with IL-2-dependent immuno deficiency.
Petrov
R V
RV
Koval'chuk
L V
LV
Kalinina
A R
AR
Pavliuk
A S
AS
Ul'ianova
T Iu
TIu
D000293:Adolescent; D000328:Adult; D001782:Blood Donors; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007376:Interleukin-2 / Q000172:deficiency* / Q000276:immunology; D007635:Keratitis, Dendritic / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008222:Lymphokines / Q000494:pharmacology*
D007376:Interleukin-2; D008222:Lymphokines
Otsenka funktsional'noĭ aktivnosti limfokinaktivirovannykh killerov u zdorovykh donorov i bol'nykh s interleĭkin-2-zavisimym immunodefitsitom.