CD8+CD4- lymphocyte lines can harbor the AIDS virus in vitro.
A detailed definition of AIDS virus-specific T lymphocytes will require the generation and characterization of HIV-1-specific, cloned T cell populations. In our studies, we show that CD8+CD4- lymphocyte lines, derived from PBL of rhesus monkeys infected with simian immunodeficiency virus of macaques and humans infected with HIV-1, can harbor AIDS viruses. CD8+CD4- lymphocyte lines derived from infected individuals are shown to express AIDS virus-encoded proteins and generate reverse transcriptase activity. Infection of these CD8+CD4- lymphocytes is confirmed at the single cell level by the techniques of immunoelectronmicroscopy and two-color immunohistochemistry. This observation suggests that it may prove problematic to generate cloned, functional T lymphocyte populations from AIDS virus-infected individuals and raises the possibility that CD8+CD4- cells may serve as reservoirs for the AIDS viruses.
Tsubota
H
H
Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.
Ringler
D J
DJ
Kannagi
M
M
King
N W
NW
Solomon
K R
KR
MacKey
J J
JJ
Walsh
D G
DG
Letvin
N L
NL
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D015497:HIV-1 / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D008253:Macaca mulatta; D010641:Phenotype; D012191:Retroviridae Proteins / Q000096:biosynthesis; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification* / Q000382:microbiology / Q000648:ultrastructure; D014779:Virus Replication
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D012191:Retroviridae Proteins