Luminescence biosensors.
A novel optical biosensor for homogeneous immunoassay has been developed on the basis of the finding that electrochemical luminescence of pyrene-labelled antigen is extremely inhibited by immunochemical complexation. Electrochemical luminescence homogeneous immunoassay for human serum albumin (HSA), as a model analyte, was performed with a platinum plate electrode which was located in the vicinity of an optical fibre tip. HSA was determined in the concentration range of 3-25 X 10(-6) mol/l. To improve electrochemical luminescence measurement an optical fibre electrode has been developed by fabricating a transparent platinum film on the top of an optical fibre. The minimum detectable limit of luminol was 10(-11) mol/l with the optical fibre electrode. Luminol was applied as a label for homogeneous immunoassay.
Aizawa
M
M
Department of Bioengineering, Tokyo Institute of Technology, Japan.
Tanaka
M
M
Ikariyama
Y
Y
Shinohara
H
H
D015374:Biosensing Techniques; D004563:Electrochemistry; D005336:Fiber Optic Technology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements; D008165:Luminol; D011721:Pyrenes; D012709:Serum Albumin / Q000032:analysis
D011721:Pyrenes; D012709:Serum Albumin; D008165:Luminol; C030984:pyrene