[Screening for irregular erythrocyte antibodies].
The existence of 20 erythrocytic receptors carried out simultaneously by indirect antiglobulin, agglutination and 3 enzyme tests gave evidence that the most sensitive enzyme test is the ficin test. The enzyme tests reliably detect antibodies of the Rh and P systems with better results than indirect antiglobulin test. They are not suitable for determining the characters of the next seven erythrocytic systems as they disintegrate partly or fully the serologically active structures of the characters. For this purpose the indirect antiglobulin tests is the most reliable reaction for detecting incomplete antibodies. When a mixture of panel erythrocytes was used in screening, a mixture taken from 3 persons proved better than that of 2, as it was possible to achieve a more suitable combination of minimum quantity of receptors needed to attain for optimum sensitivity of the reaction. It was equally proved that the receptive ability of the erythrocytes does not only depend on homo- or heterozygote characters but also on their specific relationship to the reacting antibody.
Herzog
P
P
D001789:Blood Group Antigens / Q000276:immunology*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D006801:Humans
D001789:Blood Group Antigens
Skrínink nepravidelných erytrocytárních protilátek.