Long-term growth of malignant thymocytes in vitro.
We report a new methodology for the long-term growth of malignant T-lymphoblasts from patients with T-cell acute lymphoblastic leukemia (T-ALL) and T-cell lymphoblastic lymphoma (T-LL). When malignant cells were cultured in the presence of insulin-like growth factor I under hypoxic conditions, cellular proliferation occurred that resulted in the establishment of immortal cell lines from ten of 12 patient tumors. Authenticity of each cell line was verified by a direct comparison of the immunophenotype, karyotype, and immunogenotype with the patient's tumor cells. This improved method of cell culture permits frequent establishment of cell lines from patients with T-ALL/T-LL, thereby aiding in analysis of thymocyte transformation and neoplasia.
Smith
S D
SD
Department of Pediatrics, Stanford University Medical School, Palo Alto, CA 94304-2099.
McFall
P
P
Morgan
R
R
Link
M
M
Hecht
F
F
Cleary
M
M
Sklar
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015139:Blotting, Southern; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004253:DNA Nucleotidylexotransferase / Q000032:analysis; D015321:Gene Rearrangement; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007621:Karyotyping; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008297:Male; D010641:Phenotype; D013601:T-Lymphocytes / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000276:immunology / Q000473:pathology*
D000945:Antigens, Differentiation, T-Lymphocyte; D007334:Insulin-Like Growth Factor I; D004253:DNA Nucleotidylexotransferase