Lymphocyte activation with release of soluble mediators induced by Thermoactinomyces vulgaris in vitro.
Murine spleen lymphocytes stimulated in vitro with a hypersensitivity pneumonitis-associated bacterium, Thermoactinomyces vulgaris, were found to secrete interleukin-2 up to 7 days after mitomycin C blockade. They exerted helper effect in secondary mitogen or antigen-induced lymphocyte proliferation. Cyclosporin A, an inhibitor of interleukin-2 synthesis, caused a complete abrogation of the helper effect, suggesting that the effect was mainly due to interleukin-2. Indomethacin, an inhibitor of prostaglandin synthesis, enhanced the helper effect in some inbred strains of mice, indicating prostaglandin-dependent downregulation. The strain variation in the prostaglandin-induced downregulation was not H-2 linked.
Jägerroos
H J
HJ
Department of Clinical Microbiology, University of Kuopio, Finland.
Nuortio
L T
LT
Mäntyjärvi
R A
RA
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D003524:Cyclosporins / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007376:Interleukin-2 / Q000378:metabolism*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008848:Micromonosporaceae / Q000276:immunology*
D003524:Cyclosporins; D007376:Interleukin-2; D007213:Indomethacin