T cells from eosinophilic patients produce interleukin-5 with interleukin-2 stimulation.
Anti-murine (m) interleukin-5 (IL-5) antibody was found to inhibit eosinophil (Eo) colony formation stimulated by recombinant human (rh) IL-5, but did not inhibit the production of Eo stimulated by rh IL-3 or granulocyte-macrophage colony-stimulating factor (GM-CSF). Conditioned medium (CM) prepared from eosinophilic patients' T cells with interleukin-2 (IL-2) stimulation (T-IL-2-CM), was found to contain CFU-Eo growth-stimulating factor. Using anti-mIL-5 antibody, we demonstrated that T-IL-2-CM from patients with eosinophilia contained a significant amount of IL-5. We also detected IL-5 mRNA in T cells from eosinophilic patients with IL-2 stimulation. These results suggest that IL-5 plays an important role in the induction of selective eosinophilia in humans and that IL-5 is produced from T cells with IL-2 stimulation.
Enokihara
H
H
Third Department of Internal Medicine, Dokkyo University School of Medicine, Tochigi, Japan.
Furusawa
S
S
Nakakubo
H
H
Kajitani
H
H
Nagashima
S
S
Saito
K
K
Shishido
H
H
Hitoshi
Y
Y
Takatsu
K
K
Noma
T
T
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000502:physiology; D001667:Binding, Competitive; D002648:Child; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004802:Eosinophilia / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004804:Eosinophils / Q000473:pathology; D005260:Female; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D015848:Interleukin-5; D007378:Interleukins / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D006133:Growth Substances; D007376:Interleukin-2; D015848:Interleukin-5; D007378:Interleukins; D012333:RNA, Messenger; D011994:Recombinant Proteins