Analysis for alpha-amylase isoenzymes by automated isoelectric focusing.
We describe the fractionation of alpha-amylase (EC 3.2.1.1) isoenzymes by use of the Pharmacia "Phastsystem" electrophoresis apparatus. The separation is done by isoelectric focusing on "Phastgel IEF 5-8." A complete run, including staining, takes 4 h and can easily resolve eight isoenzymes. Samples can be analyzed in a routine laboratory with use of conventional reagents.
Lessard
F
F
Service de biochimie, Hôpital Saint-Luc, Montréal, Canada.
Dion
R
R
D001322:Autoanalysis; D002624:Chemistry, Clinical; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D010189:Pancreatic Juice / Q000201:enzymology; D012463:Saliva / Q000201:enzymology; D000516:alpha-Amylases / Q000302:isolation & purification* / Q000378:metabolism
D007527:Isoenzymes; D000516:alpha-Amylases