title: Dietary interactions of lima bean (Phaseolus lunatus) trypsin inhibitor, haemagglutinin and cyanide. Part 2. Effect on pancreatic and intestinal alpha-amylase (EC 3.21.1.1) in growing albino rats.
abstract: A total of 108 growing albino rats was used to evaluate the dietary interactions of the major lima bean antinutritional factors trypsin inhibitor (TI), haemagglutinin (Hgg) and cyanide (CN) with respect to their effects on pancreatic and intestinal alpha-amylase activities. The results indicate that when fed at the same level of activity as found in the raw lima bean (RLB) these factors had no significant (p greater than 0.05) influence on pancreatic alpha-amylase activity whether acting individually or in combination. However, when acting alone, CN appeared to depress pancreatic amylase level more than when interacting with TI or Hgg or both. Amylase activity was significantly (p less than 0.01) depressed by the dietary treatments in both the small and large intestine while caecal levels were not. The most severe depression in amylase activity was elicited by the RLB diet. The haemagglutinin-containing diets appeared generally associated with lower levels of intestinal amylase activity. From the present finding it is suggested that these factors alone cannot fully account for the magnitude of the depression of intestinal amylase activity which is contingent upon the ingestion of RLB by experimental rats.
authors: Department of Animal Production and Health, Federal University of Technology, Akure, Nigeria.
authors: V A
authors: VA
authors: Aletor
mesh_terms: D000818:Animals; D003486:Cyanides / Q000494:pharmacology*; D004032:Diet; D005504:Food Analysis; D006388:Hemagglutinins / Q000032:analysis*; D007422:Intestines / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology*; D010940:Plant Proteins; D011480:Protease Inhibitors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D003486:Cyanides; D006388:Hemagglutinins; D010940:Plant Proteins; D011480:Protease Inhibitors; C025398:protease inhibitor protein, Phaseolus lunatus; D000516:alpha-Amylases