title: Loss of copper-zinc superoxide dismutase gene expression in differentiated cells of myelo-monocytic origin.
abstract: Changes in the production of reactive oxygen species and total superoxide dismutase activity have been observed during differentiation of some hematopoietic cells. We therefore investigated whether the steady-state level and rate of transcription of superoxide dismutase-1 (SOD-1) mRNA change during terminal differentiation of the human leukemia cell lines THP-1, HEL, and HL-60 into macrophages and/or granulocytes, respectively. Macrophage differentiation is accompanied by a gradual decrease in both the transcription rate (10x) and the steady-state level (6x) of SOD-1 mRNA. No decrease was observed after treatment with the diacylglycerol analog 1,2 dioctanol-rac-glycerol (di-C8), which like phorbol 12-myristate 13-acetate also activates protein kinase C but does not induce differentiation at the concentration used. The same decrease in SOD-1 mRNA level was observed when HL-60 cells were induced to differentiate into granulocytes by treatment with dimethylsulfoxide. These data suggest that a decrease in SOD-1 mRNA to almost undetectable levels accompanies differentiation of macrophages and granulocytes.
authors: Division of Metabolism, Endocrinology, and Nutrition, University of Washington, Seattle.
authors: J H
authors: JH
authors: Auwerx
authors: A
authors: A
authors: Chait
authors: G
authors: G
authors: Wolfbauer
authors: S S
authors: SS
authors: Deeb
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004075:Diglycerides / Q000494:pharmacology; D015870:Gene Expression; D005796:Genes; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D015473:Leukemia, Promyelocytic, Acute; D008264:Macrophages / Q000166:cytology / Q000201:enzymology; D009000:Monocytes; D012333:RNA, Messenger / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D004075:Diglycerides; D012333:RNA, Messenger; C044295:1,2-dioctanoylglycerol; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate