[Transfer of the agrobacterial gene for cytokinin biosynthesis into tobacco plants].
The gene transfer into plants using the genetic engineering methods gives us the possibility to obtain transgeneric plants having acquired the new traits. Some bacterial genes can be used for this purpose. Obtaining of a transgeneric plant harbouring the cytokinin synthesis gene ipt (gene 4) from the T-DNA of Agrobacterium tumefaciens Ti-plasmid seems to be useful. The expression of tumor agrobacterial ipt gene in transformed plant cells interferes with the normal growth and regulation of the whole plant. The successful transfer of the cloned ipt gene from the recombinant plasmid pGV0319 into the tobacco plant using Agrobacterium vectors and succeeding regeneration of phenotypically normal transgenic plants are reported in the present paper.
Iusibov
V M
VM
Pogosian
G P
GP
Andrianov
V M
VM
Piruzian
E S
ES
D003583:Cytokinins / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial; D005818:Genetic Engineering; D010937:Plant Growth Regulators / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
D003583:Cytokinins; D010937:Plant Growth Regulators
Perenos v rasteniia tabaka agrobakterial'nogo gena biosinteza tsitokinina.