Comparison of DNA breaks in diabetic dogs and galactosemic dogs developing retinopathy.
DNA strand breaks have been evaluated in alloxan-diabetic dogs and experimentally galactosemic dogs, both animal models known to develop a retinopathy similar to that in diabetic patients. Lymphocytes were used as the source of DNA, and the presence of strand breaks was detected by monitoring the rate of alkali-induced DNA unwinding. The rate of DNA unwinding from dogs diabetic or galactosemic for up to 4 years was not significantly different from normal. These findings lend no support to previous reports that DNA strand breaks increase in diabetes or hyperglycemia. Retinopathy apparently can develop in the absence of DNA strand breaks, at least as estimated from lymphocytes.
Kern
T S
TS
Department of Ophthalmology, University of Wisconsin, Madison 53706.
Engerman
R L
RL
D000818:Animals; D004249:DNA Damage; D003921:Diabetes Mellitus, Experimental / Q000235:genetics*; D003930:Diabetic Retinopathy / Q000235:genetics*; D004285:Dogs; D005456:Fluorescent Dyes; D005693:Galactosemias / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology; D012164:Retinal Diseases / Q000209:etiology*; D013997:Time Factors
D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide