title: A role for the thymic epithelium in the selection of pre-T cells from murine bone marrow.
abstract: A rat thymic epithelial cell line IT45-R1 has been previously described as secreting soluble molecules that in vitro chemoattract rat hemopoietic precursor cells. The development of such an in vitro migration assay was based on the ability of cells to migrate across polycarbonate filters in Boyden chambers. In the present paper, by using the same strategy, we studied murine bone marrow cells capable of migrating in vitro toward IT45-R1 conditioned medium. The responding cells were shown to represent a minor bone marrow subpopulation characterized by a low capacity to incorporate tritiated thymidine in vitro (less than 10% of control). Moreover, this cell subset was considerably impoverished with respect to granulocyte-macrophage CFU (less than 7% of control) and pluripotent hemopoietic stem cells (less than 12% of control). Potential generation of T cells of donor-type in the lymphoid organs of irradiated recipients was measured by using C57BL/Ka Thy-1.1 and Thy-1.2 congenic mice. Thy-1.1 irradiated mice were injected intrathymically or intravenously with the selectively migrated cell subset of Thy-1.2 donor-type bone marrow cells. The use of an i.v. transfer route allowed us to show that these cells possess thymus-homing and colonization abilities. In a time-course study after intrathymic cell transfer, these migrated cells were able to generate Thy-1.2+ donor-type thymocytes represented by all cortical and medullary cell subsets in a single wave of repopulation from day 20 to day 30 after transfer, with a peak around days 23 to 25. The degree of repopulation closely resembled that seen with unfractionated bone marrow cells in terms of absolute numbers of donor cells per thymus (82% of control, 22 x 10(6) Thy-1.2+ cells) as well as in percent donor cells per thymus (105% of control). Thy-1.2+ cells were also detected in the lymph nodes and the spleens of reconstituted recipient mice. Taken together, these results support the idea that the supernatant of the established thymic epithelium IT45-R1 induces the migration of a murine bone marrow subset that contains hemopoietic stem cells already committed to the lymphoid lineage (i.e., pre-T cells).
authors: Laboratoire de Physiopathologie du Développement, CNRS URA 230, Paris, France.
authors: B
authors: B
authors: Bauvois
authors: S
authors: S
authors: Ezine
authors: B
authors: B
authors: Imhof
authors: M
authors: M
authors: Denoyelle
authors: J P
authors: JP
authors: Thiery
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D002465:Cell Movement; D002469:Cell Separation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004848:Epithelium / Q000502:physiology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000502:physiology*; D007116:Immunization, Passive; D008198:Lymph Nodes; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D013154:Spleen; D013601:T-Lymphocytes / Q000145:classification / Q000502:physiology* / Q000637:transplantation; D013950:Thymus Gland / Q000502:physiology*