title: The effect of hypervolemic hemodilution with and without hypertension on cerebral blood flow following middle cerebral artery occlusion in rats anesthetized with isoflurane.
abstract: The effect of hypervolemic hemodilution or hypervolemic hemodilution with dopamine-induced hypertension on cerebral blood flow (CBF) was investigated during 1.2 MAC isoflurane anesthesia in rats (n = 24) subjected to middle cerebral artery occlusion (MCAO). Prior to MCAO each animal was randomized to one of the following groups: 1) control, mean arterial pressure (89 +/- 10 mmHg [mean +/- SD]), blood volume, and hematocrit (46 +/- 1) were not manipulated; 2) hypervolemic hemodilution (HH), 30 min before MCAO, 5% albumin was administered to reduce the hematocrit to 29-32%; or 3) hypervolemic hemodilution/dopamine hypertension (HH/Dop), hemodilution was accomplished and dopamine (10 micrograms.kg-1.min-1) was infused during the ischemic period to achieve a mean arterial pressure of 111 +/- 10 mmHg (mean +/- SD). Ten minutes after occlusion of the left middle cerebral artery, CBF was determined using 14C-iodoantipyrine. Five coronal brain sections were analyzed to determine the area within each brain section with CBF ranges of 0-15 ml.100 g-1.min-1 and 15-23 ml.100 g-1.min-1. The area of 0-15 ml.100 g-1.min-1 CBF was less in both the HH and HH/Dop groups compared with control (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Anesthesiology, Loma Linda University, California 92354.
authors: D J
authors: DJ
authors: Cole
authors: J C
authors: JC
authors: Drummond
authors: H M
authors: HM
authors: Shapiro
authors: R E
authors: RE
authors: Hertzog
authors: F S
authors: FS
authors: Brauer
mesh_terms: D000769:Anesthesia, Inhalation; D000818:Animals; D001157:Arterial Occlusive Diseases / Q000503:physiopathology / Q000628:therapy; D001794:Blood Pressure / Q000187:drug effects; D001810:Blood Volume; D002539:Cerebral Arterial Diseases / Q000503:physiopathology* / Q000628:therapy; D002560:Cerebrovascular Circulation; D004298:Dopamine / Q000494:pharmacology; D006438:Hemodilution; D007530:Isoflurane; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007530:Isoflurane; D004298:Dopamine