Intracellular acidification induces decrease of cytosolic calcium in isolated osteoclasts.
In this study the effects of changes in intracellular pH on cytosolic Ca2+ were examined in single isolated osteoclasts. Alkalinization, performed by incubation in HCO3 containing buffer, induced increases in [Ca2+]. Conversely acidification, obtained by incubation in Na-butyrate-containing buffer induced a rapid and sustained decrease of [Ca2+]. The decrease of [Ca2+], during acidification with Na-butyrate was inhibited by VO4, an inhibitor of the Ca2+-ATPase. All these pieces of information indicate that changes in pH1 can modulate osteoclast activity together with modifications of [Ca2+], probably acting as intracellular signals.
Teti
A
A
Grano
M
M
Teitelbaum
S L
SL
Hruska
K A
KA
Colucci
S
S
Zambonin Zallone
A
A
D000818:Animals; D001717:Birds; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010010:Osteoclasts / Q000378:metabolism*
D002118:Calcium