Interleukin 2 promotes expression of an interleukin 2 receptor and proliferation of T cells stimulated with non-mitogenic dose of concanavalin A.
Interleukin 2 (IL-2) initiated proliferation of the cells of T-enriched population stimulated with non-mitogenic dose of concanavalin A (Con A), whereas it could not induce proliferation of the cells treated with non-mitogenic lectin wheat germ agglutinin (WGA). Use of monoclonal antibody (MAb) directed against interleukin 2 receptor (IL-2R) showed that IL-2 significantly increased expression of IL-2R on the cells of T-enriched population stimulated with Con A, whereas it could not cause any significant enhancement of expression of IL-2R on the cells treated with WGA. We concluded that IL-2 activated T cells in combination with non-mitogenic does of Con A, and induced both expression of IL-2R and proliferation.
Komada
H
H
Department of Pathology, Mie University School of Medicine, Japan.
Nakabayashi
H
H
Hara
M
M
Hara
M
M
Takahashi
T
T
Morita
J
J
Yoshida
T
T
Takanari
H
H
Izutsu
K
K
D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology*; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A