Immunosuppressive factor derived from renal cancer cells.
An immunosuppressive factor derived from conditioned medium of a human renal cancer cell line, KU2, was analyzed, using mitogen-activated normal peripheral blood lymphocytes. Addition of conditioned medium to lymphocyte cultures resulted in suppression of [3H]-thymidine incorporation to DNA of lymphocytes in a dose-dependent manner. This effect was cytostatic and reversible. This factor inhibited lymphocyte activation stimulated by mitogen at an early stage, and it also suppressed Interleukin-2 production by activated lymphocytes. This factor was non-dialyzable and heat sensitive at 56 degrees C. DNA replication of the cells was necessary for the production of this factor.
Muraki
J
J
Department of Urology, New York Medical College, Valhalla.
Fischer
J
J
Addonizio
J C
JC
Nagamatsu
G R
GR
Chiao
J W
JW
D002292:Carcinoma, Renal Cell / Q000276:immunology*; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D007680:Kidney Neoplasms / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured / Q000032:analysis
D003470:Culture Media; D007376:Interleukin-2; D013491:Suppressor Factors, Immunologic