[Determination of epoxide hydrolase activity by high performance liquid chromatography].
A new highly sensitive method of microsomal epoxide hydrolase activity has been worked out. The product of styrene oxide enzyme hydrolysis--phenylethyleneglycol (PEG) was extracted by n-butanol, and its aliquot was analysed by high-performance liquid-chromatography on Silica Gel using hexane-isopropanol-water (80:28:2) mobile phase. PEG was detected at 210 nm. The detection limit of PEG was 5 pmol per injection. The coefficient of variation of the method was 3.7%.
Kravchenko
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LV
Sobolev
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VS
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004851:Epoxide Hydrolases / Q000032:analysis*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D004851:Epoxide Hydrolases
Opredelenie aktivnosti époksidgidrolazy s ispol'zovaniem vysokoéffektivnoĭ zhidkostnoĭ khromatografii.