Isolation and gas chromatographic determination of chlorsulfuron in milk.
A method for the isolation and gas chromatographic determination of chlorsulfuron in milk is presented. Blank or chlorsulfuron-spiked milk samples were blended into C-18 (octadecylsilyl derivatized silica, ODS) packing material. A column made from the C-18/milk matrix was washed with hexane after which chlorsulfuron was eluted with dichloromethane (DCM). The DCM eluate contained chlorsulfuron which was free from interfering co-extractants when analyzed by gas chromatography utilizing a nitrogen/phosphorus detector. Chlorsulfuron was found to undergo a thermally induced decomposition to give 2-amino-4-methoxy-6-methyl-1,3,5-triazine, which was detected and quantitated by this method. Standard curves for these analyses were linear (r = 0.992 +/- 0.004, n = 5), with an average percentage recovery of 91.6 +/- 10.8%, over the concentration range examined (62.5-2000 ng/mL). The inter- and intra-assay variabilities were 11.6 +/- 7.5% and 6.2%, respectively.
Long
A R
AR
Louisiana State University, School of Veterinary Medicine, Department of Veterinary Physiology, Pharmacology and Toxicology, Baton Rouge 70803.
Hsieh
L C
LC
Malbrough
M S
MS
Short
C R
CR
Barker
S A
SA
D000818:Animals; D002417:Cattle; D006540:Herbicides / Q000032:analysis*; D007202:Indicators and Reagents; D008892:Milk / Q000032:analysis*; D012015:Reference Standards; D013449:Sulfonamides; D014227:Triazines / Q000032:analysis*
D006540:Herbicides; D007202:Indicators and Reagents; D013449:Sulfonamides; D014227:Triazines; C037137:chlorsulfuron