Cytoplasmic alkalinization produced by the combination of anti-immunoglobulin antibody plus cytochalasin D in murine B lymphocytes.
The intracellular pH of murine splenic B lymphocytes was measured using the pH-sensitive fluorescent dye, bis(carboxyethyl)carboxyfluorescein (BCECF). After stimulation of B lymphocytes with anti-immunoglobulin antibody plus cytochalasin D, two agents that act in synergy to promote S-phase entry, a late increase in pH was detected that occurred prior to the onset of DNA synthesis. The degree of alkalinization observed was comparable to that produced by two additional mitogenic regimens. Cytoplasmic alkalinization was not blocked by dimethylamiloride. Cytoplasmic alkalinization represents a sign of, and may play a role in, stimulation of B lymphocytes to enter S phase.
van Haelst-Pisani
C
C
Evans Memorial Department of Clinical Research, Boston University Medical Center, Massachusetts 02118.
Cragoe
E J
EJ
Rothstein
T L
TL
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D015638:Cytochalasin D; D003572:Cytochalasins / Q000494:pharmacology*; D005452:Fluoresceins; D006863:Hydrogen-Ion Concentration; D007399:Interphase / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D013936:Thymidine / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D003572:Cytochalasins; D005452:Fluoresceins; D015638:Cytochalasin D; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D013936:Thymidine