Susceptibility of human male keratinocytes to MHC-restricted H-Y-specific lysis.
We studied the expression of the male-specific mH antigen H-Y on cultured human skin cells by investigating susceptibility to H-Y-specific cytolysis using conventional class I-restricted CTL clones in a modified cell-mediated cytotoxicity assay. In contrast to what was found in the rodent system, we observed H-Y-specific lysis of human male keratinocytes. Susceptibility for H-Y-specific lysis was efficiently enhanced by exposure of the keratinocytes to IFN-gamma. Our results demonstrate that human skin cells are equally sensitive for the activity of H-Y-specific CTLs as target cells of lymphoid origin. Finally, the cellular recognition of the H-Y mH antigen in the skin further supports its possible target function in the local graft versus host attack.
van Els
C A
CA
Department of Immunohematology and Bloodbank, University Hospital Leiden, The Netherlands.
de Bueger
M M
MM
Kempenaar
J
J
Ponec
M
M
Goulmy
E
E
D003602:Cytotoxicity, Immunologic; D006182:H-Y Antigen / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology; D015235:HLA-B Antigens / Q000276:immunology; D006801:Humans; D015603:Keratinocytes / Q000276:immunology*; D008285:Major Histocompatibility Complex; D008297:Male; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D006182:H-Y Antigen; D015234:HLA-A Antigens; D015235:HLA-B Antigens