title: [Identification of a peptide of the membrane protein of tobacco mosaic virus, cross-linked with intraviral RNA under the effect of UV-radiation].
abstract: As reported previously, UV-irradiation induces crosslinking between tobacco mosaic virus (TMV) coat protein molecules and intraviral RNA nucleotides. We have irradiated [3H]-uridine labeled TMV and isolated TMV coat protein subunits with the attached nucleotide label. These TMV protein subunits were hydrolyzed with trypsin. The tryptic peptides were separated by high-performance liquid chromatography and [3H]-labeled peptides were identified. The UV-irradiation of TMV was found to result in crosslinking to intraviral RNA of the T8 tryptic peptide (residues 93-112) of TMV coat protein.
authors: N I
authors: NI
authors: Grebenshchikov
authors: Z Kh
authors: ZKh
authors: Arbieva
authors: E N
authors: EN
authors: Dobrov
mesh_terms: D002213:Capsid / Q000378:metabolism* / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D010455:Peptides / Q000032:analysis*; D012367:RNA, Viral / Q000378:metabolism* / Q000528:radiation effects; D014027:Tobacco Mosaic Virus / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D010455:Peptides; D012367:RNA, Viral
vernacular_title: Identifikatsiia peptida belka obolochki virusa tabanoĭ mozaiki, sshivaiushchegosiia s vnutrivirusnoĭ RNK pod deĭstviem UF- izlucheniia.