title: Capillary tube isotachophoresis of proteins in early postmortem serum, cerebrospinal fluid and pericardial fluid.
abstract: Serum, cerebrospinal fluid (CSF) and pericardial fluid (PDF) obtained from 22 early cadavers were subjected to capillary tube isotachophoresis. Separated proteins were grouped into three main fractions, namely, Fr. 1, Fr. 2 and Fr. 3, in order of their decreasing effective (net) mobilities. The percentages of main fractions to total content were calculated by UV absorbance integration. It was found that Fr. 1 decreased and Fr. 2 increased in all three kinds of specimens with an increase in time following death. In serum and also in CSF, the degree of these changes between 6 and 58 hours following death seemed large enough (about 30%) to be used for crude estimations of early postmortem intervals. Changes in PDF proceeded more gradually than in serum and or in CSF, with only 5-20% changes during this period.
authors: K
authors: K
authors: Takahama
authors: Y
authors: Y
authors: Seo
authors: S
authors: S
authors: Tomono
authors: N
authors: N
authors: Yukawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001797:Blood Protein Electrophoresis; D001826:Body Fluids / Q000032:analysis*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008875:Middle Aged; D010496:Pericardium; D011180:Postmortem Changes; D011506:Proteins / Q000032:analysis*; D013997:Time Factors
chemical_list: D002556:Cerebrospinal Fluid Proteins; D011506:Proteins