title: Molecular cloning and characterization of the met2 gene from Ascobolus immersus.
abstract: We have cloned the met2 gene from Ascobolus immersus by heterologous hybridization with the MET2 gene of Saccharomyces cerevisiae. This gene codes for the homoserine O-transacetylase, one of the methionine biosynthetic enzymes. The complete nucleotide sequence of a 2910-bp DNA fragment carrying the met2 gene has been determined. The gene contains a 165-bp intron which is similar in structure to other fungal introns. The deduced amino acid (aa) sequence (518 aa residues; Mr of 57726) shows three domains with a significant level of homology with the corresponding yeast protein. Northern-blot analysis reveals at least two transcripts (2.4 and 2.1 kb) probably due to transcription termination heterogeneity, as suggested by S1-mapping experiments. Polymorphism has been observed in the met2 gene flanking regions of Ascobolus strains from two different stocks.
authors.affiliation: Laboratoire I.M.G., Université Paris-Sud, Orsay, France.
authors.forename: C
authors.initials: C
authors.lastname: Goyon
authors.forename: G
authors.initials: G
authors.lastname: Faugeron
authors.forename: J L
authors.initials: JL
authors.lastname: Rossignol
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics
chemical_list: D003062:Codon; D004271:DNA, Fungal; D000123:Acetyltransferases; C021369:homoserine O-acetyltransferase; D004262:DNA Restriction Enzymes