Role of alpha-adrenoceptor subtypes mediating sympathetic vasoconstriction in human digits.
The effects of intra-arterial administration of alpha-1 and alpha-2 adrenoceptor agonists and antagonists on human digital blood flow were studied before and during reflex sympathetic vasoconstriction in normal subjects. Total finger flow was measured by venous occlusion air plethysmography and capillary flow by the disappearance rate of a radioisotope from a local injection in a fingerpad. Intra-arterial phenylephrine (0.2-1.3 micrograms min-1) and clonidine (0.12-0.48 micrograms min-1) produced dose-related decreases in finger blood flow and increases in vascular resistance. Clonidine was the more potent vasoconstrictor. Prazosin (0.4-3 micrograms min-1) effectively blocked the vasoconstrictor effect of phenylephrine but not clonidine, while yohimbine (30-70 micrograms min-1) blocked the effect of clonidine but not phenylephrine. In a 20 degrees C room, prazosin (0.4-13.2 micrograms min-1) caused no significant changes in finger blood flow (7.7 +/- 2.1 to 11.7 +/- 3.3 ml min-1 100 ml-1) or vascular resistance (30.9 +/- 8.8 to 28.1 +/- 8.7 mmHg ml-1 min-1 100 ml-1). In the 20 degrees C room, yohimbine (30-70 micrograms min-1) produced a significant increase in finger blood flow (7.8 +/- 2.8 to 23.4 +/- 6.8 ml, P less than 0.01) and decrease in vascular resistance (20.5 +/- 5.7 to 6.0 +/- 2.2 units, P less than 0.01). No significant changes occurred in finger capillary flow with prazosin or yohimbine infusions. It is concluded that alpha-1 and alpha-2 adrenoceptors are present in human digital vasculature and that alpha-2 adrenoceptors are more important than alpha-1 adrenoceptors in sympathetic neural vasoconstriction.(ABSTRACT TRUNCATED AT 250 WORDS)
Coffman
J D
JD
Evans Memorial Department of Clinical Research, University Hospital, Boston University Medical Center, MA.
Cohen
R A
RA
D000328:Adult; D003000:Clonidine / Q000494:pharmacology; D005260:Female; D005385:Fingers / Q000098:blood supply*; D006801:Humans; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000502:physiology*; D014661:Vasoconstriction; D015016:Yohimbine / Q000494:pharmacology
D011942:Receptors, Adrenergic, alpha; D010656:Phenylephrine; D015016:Yohimbine; D003000:Clonidine; D011224:Prazosin