Glycine-evoked release of [3H]acetylcholine from rat striatal slices is independent of the NMDA receptor.
KCl-, NMDA-, and glycine-evoked release of [3H]acetylcholine was studied in superfused rat striatal slices. KCl-evoked release of [3H]acetylcholine was inhibited by 1.2 mM MgCl2 and 100 microM lidocaine. Similarly, NMDA-evoked release was inhibited by MgCl2 and lidocaine as well as 10 microM CGS 19755, a competitive antagonist at NMDA receptors, and 10 nM MK-801, a noncompetitive antagonist of NMDA-induced responses. Glycine-evoked release was calcium-dependent and was inhibited by 0.1 microM strychnine whereas KCl- and NMDA-evoked release were resistant to strychnine. In addition, lidocaine inhibited the glycine-induced response. Cross-tachyphylaxis was not observed between NMDA- and glycine-evoked release. These results indicate that the strychnine-sensitive, glycine-evoked release of [3H]acetylcholine is independent of the NMDA receptor.
Taylor
C A
CA
Ciba-Geigy Corp., Pharmaceuticals Division, Summit, NJ 07901.
Tsai
C
C
Lehmann
J
J
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D005998:Glycine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013331:Strychnine / Q000494:pharmacology
D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D011189:Potassium Chloride; D013331:Strychnine; D000109:Acetylcholine; D005998:Glycine