Modulation of retinal transducin and phosphodiesterase activities by synthetic peptides of the phosphodiesterase gamma-subunit.
Synthetic peptides corresponding to various regions of the light-activated guanosine 3',5'-cyclic monophosphate phosphodiesterase (PDE) gamma-subunit (PDE gamma) from bovine retinal rod outer segments were synthesized and tested for their ability to inhibit PDE activity, and GTPase activity of transducin. One of these peptides, corresponding to PDE gamma residues 31-45, inhibited PDE activity and GTPase activity in a dose-dependent manner. The GTPase activity was inhibited by PDE gamma-3 non-competitively. This region of the PDE gamma subunit may be involved in the direct interaction of transducin and PDE alpha beta with PDE gamma.
Morrison
D F
DF
Department of Biochemistry, Kansas State University, Manhattan 66506.
Rider
M A
MA
Takemoto
D J
DJ
D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D020558:GTP Phosphohydrolases / Q000037:antagonists & inhibitors; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010786:Photoreceptor Cells / Q000378:metabolism*; D012374:Rod Cell Outer Segment / Q000201:enzymology / Q000378:metabolism*; D015293:Transducin
D008565:Membrane Proteins; D010455:Peptides; D010726:Phosphodiesterase Inhibitors; D010727:Phosphoric Diester Hydrolases; D020558:GTP Phosphohydrolases; D015293:Transducin