The trisaccharide beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp, as its 8-methoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V.
Incubation of the trisaccharide acceptor, beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp-O( CH2)8CO2Me with sonicates of Rous sarcoma-transformed baby-hamster kidney cells, which contain N-acetylglucosaminyltransferase V activity, resulted in the production of beta-D-GlcpNAc-(1----2)-[beta-D-GlcpNAc-(1----6)]-alpha-D-Manp-(1- ---6)- beta-D-Manp-O(CH2)8CO2Me (4). The product of the enzymic reaction was identified by comparison of its 1H-n.m.r. spectrum with that of authentic 4 whose chemical synthesis is also described.
Hindsgaul
O
O
Department of Chemistry, University of Alberta, Edmonton, Canada.
Tahir
S H
SH
Srivastava
O P
OP
Pierce
M
M
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D007202:Indicators and Reagents; D007668:Kidney; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014312:Trisaccharides / Q000378:metabolism*
D007202:Indicators and Reagents; D014312:Trisaccharides