title: Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein.
abstract: Vectors were constructed that allow foreign peptides to be expressed in Escherichia coli as fusion proteins. The peptides are fused to the C terminus of maltose-binding protein (MBP), which allows them to be purified by the MBP's affinity to cross-linked amylose (starch). The fusion protein can be directed to the periplasm by including the leader sequence from the phoA gene on the vector.
authors.affiliation: New England BioLabs, Beverly, MA 01915.
authors.forename: C
authors.initials: C
authors.lastname: di Guan
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.forename: P D
authors.initials: PD
authors.lastname: Riggs
authors.forename: H
authors.initials: H
authors.lastname: Inouye
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000469:Alkaline Phosphatase / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005796:Genes; D005822:Genetic Vectors; D005838:Genotype; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification*
chemical_list: D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C461599:maltose transport system, E coli; D004262:DNA Restriction Enzymes; D000469:Alkaline Phosphatase