Pseudorabies virus as a live virus vector for expression of foreign genes.
The cDNA coding for human tissue plasminogen activator (tPA) was cloned downstream from the promoter for pseudorabies virus (PRV) glycoprotein and flanked by downstream PRV DNA. After co-transfection with PRV DNA, this plasmid recombined to insert the tPA cDNA into the viral genome. In cells infected by this recombinant virus, tPA was detected by immunoprecipitation analysis and by enzymatic activity. Since it has a wide host range but does not infect humans, PRV is a possible vaccine vector for genes from animal pathogens.
Thomsen
D R
DR
Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49001.
Marotti
K R
KR
Palermo
D P
DP
Post
L E
LE
D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005822:Genetic Vectors; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics; D014612:Vaccines / Q000302:isolation & purification
D014612:Vaccines; D004247:DNA; D010959:Tissue Plasminogen Activator