Estradiol-dependent trans-acting factor binds preferentially to a dyad-symmetry structure within the third intron of the avian vitellogenin gene.
The secondary activation of the avian vitellogenin II gene in isolated liver nuclei by cytoplasmatic liver extracts of estradiol-treated chicks is accompanied by the binding of a protein from the extract to the structural part of the cloned gene. Both the DNA-binding and gene-stimulatory activities, which cochromatograph on heparin-Sepharose, are apparently present only in the cytoplasmatic liver extracts of estradiol-treated roosters and in the oviduct extracts of egg-laying hens. DNA-binding competition assays combined with exonuclease III footprinting showed that the factor binds to the imperfect dyad-symmetry structure 5'GTCTTGTTCCAAAC3' within the third intron of the gene. The factor is sequence specific and binds equally well to both single-and double-stranded DNA with an estimated dissociation constant of 3.5 X 10(-10) M.
Jost
J P
JP
Friedrich Miescher Institut, Basel, Switzerland.
Saluz
H
H
Jiricny
J
J
Moncharmont
B
B
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology*; D005092:Exonucleases / Q000494:pharmacology; D005786:Gene Expression Regulation; D007438:Introns / Q000187:drug effects*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D004856:Postural Balance; D014819:Vitellogenins / Q000235:genetics*
D004268:DNA-Binding Proteins; D014819:Vitellogenins; D004958:Estradiol; D004247:DNA; D005092:Exonucleases