Discrepancy between effects of cholera toxin on net fluid movement and cAMP levels in rat jejunum, ileum, and colon.
Regional differences in the response to cholera toxin were evaluated in rat jejunum, ileum, and colon in vivo. Ligated intestinal loops were exposed to a supramaximal concentration of cholera toxin for 5 hr, and net fluid transport, adenosine 3',5'-monophosphate (cAMP) concentrations, and adenylate cyclase and phosphodiesterase activities of mucosal homogenates were determined. The fluid transport response and the specific activities of adenylate cyclase (with and without cholera toxin) and phosphodiesterase declined progressively from the jejunum to the colon. In contrast, cAMP concentrations (with and without cholera toxin) were lowest in the jejunum and highest in the colon. These results demonstrate that cAMP concentrations of the total mucosal homogenate do not parallel cholera toxin-induced fluid secretion in the three intestinal segments. Rather, the activities of adenylate cyclase and phosphodiesterase suggest a relation between fluid secretion and the turnover of cAMP.
Farack
U M
UM
Medizinische Klinik Innenstadt, Universität München, F.R.G.
Gerzer
R
R
Keravis
T M
TM
Loeschke
K
K
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology*; D003106:Colon / Q000201:enzymology / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007082:Ileum / Q000201:enzymology / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects*; D007583:Jejunum / Q000201:enzymology / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D051381:Rats
D002772:Cholera Toxin; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases