Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein.
Mutation of the alpha-actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha-actinin gene. Transformants deficient in alpha-actinin, an actin-binding protein, produced an altered mRNA that lacked the 3' portion of the coding region. The defect in alpha-actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha-actinin sequence and its complementary sequence in the coding region of the endogenous gene.
Witke
W
W
Max-Planck-Institut für Biochemie, Martinsried, FRG.
Nellen
W
W
Noegel
A
A
D000185:Actinin / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004023:Dictyostelium / Q000235:genetics*; D005796:Genes; D005800:Genes, Fungal; D005822:Genetic Vectors; D012333:RNA, Messenger / Q000235:genetics*; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D012333:RNA, Messenger; D000185:Actinin; D004262:DNA Restriction Enzymes