title: Control of blastema cell proliferation by possible interplay of calcium and cyclic nucleotides during newt limb regeneration.
abstract: The effects of the divalent cation ionophore A23187, papaverine, and chlorpromazine on the mitotic index and cyclic nucleotide levels in newt limb regeneration blastemata (Notophthalmus viridescens) were assessed. The results of the experiments suggest that an intracellular increase in divalent cation (Ca2+) concentration results in elevated cGMP levels, suppressed cAMP levels, and a corresponding increase in blastema cell proliferation. The results also suggest that the converse conditions, namely, calcium efflux or inhibition of calmodulin activation (i.e., inhibition of Ca2+ binding), yields elevated cAMP levels, suppressed cGMP levels, and a corresponding decrease in blastema cell divisions.
authors.affiliation: Department of Biology, University of Waterloo, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Globus
authors.forename: S
authors.initials: S
authors.lastname: Vethamany-Globus
authors.forename: A
authors.initials: A
authors.lastname: Kesik
mesh_terms: D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002746:Chlorpromazine / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D006152:Cyclic GMP / Q000502:physiology*; D005121:Extremities; D008239:Lysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects; D009673:Notophthalmus viridescens / Q000502:physiology*; D009924:Organ Culture Techniques; D010208:Papaverine / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects; D012455:Salamandridae / Q000502:physiology*
chemical_list: C055277:hipppuryllsyl(N-epsilon-5'-phospho)adenosine; D000001:Calcimycin; D000249:Adenosine Monophosphate; D010208:Papaverine; D000242:Cyclic AMP; D006152:Cyclic GMP; D008239:Lysine; D002118:Calcium; D002746:Chlorpromazine