Isolation of mutants sensitive to 2-aminopurine and alkylating agents and evidence for the role of DNA methylation in Penicillium chrysogenum.
Using high performance liquid chromatography, the presence of N6-methyladenine has been found at a level of 0.1 mol percent in DNA extracted from Penicillium chrysogenum. No 5-methylcytosine was detected. A mutant strain HP547, which is sensitive to the lethal effects of N-methyl-N'-nitro-N-nitrosoguanidine, methylmethane sulphonate and the base analogue 2-aminopurine shows an increased spontaneous mutation rate and no detectable DNA methylation. Comparison of restriction enzyme digests of wild type and undermethylated strains indicated that methylation was occurring at a different sequence to that of the Dam methylase system of E. coli.
Rogers
S D
SD
School of Biotechnology, Faculty of Engineering and Science, Polytechnic of Central London, UK.
Rogers
M E
ME
Saunders
G
G
Holt
G
G
D015075:2-Aminopurine / Q000494:pharmacology*; D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis; D000477:Alkylating Agents / Q000494:pharmacology*; D004260:DNA Repair; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D008745:Methylation; D009154:Mutation; D010407:Penicillium / Q000235:genetics*; D010408:Penicillium chrysogenum / Q000187:drug effects / Q000235:genetics*
D015075:2-Aminopurine; D000225:Adenine; D000477:Alkylating Agents; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; C000730567:6-methyladenine