Effect of atrial natriuretic factor on aldosterone and its precursor steroid production in adrenal zona glomerulosa cells from spontaneously hypertensive rats.
The effect of alpha-human atrial natriuretic factor (alpha-hANP, 10(-6) M- 10(-8) M) on basal, and maximum angiotensin II (AII, 4.8 X 10(-8) M)-, ACTH (4.3 X 10(-9) M)-, and potassium (8mM)-stimulated levels of corticosterone, 18-hydroxycorticosterone (18-OHB) and aldosterone production were studied in adrenal glomerulosa cells from spontaneously hypertensive rats (SHR) at 14 weeks of age as compared to those in the age-matched Wistar-Kyoto rats (WKY) on a normal sodium diet. Plasma corticosterone, 18-OHB and aldosterone levels and the aldosterone response in vitro to the graded doses of AII were similar in SHR and WKY. Basal, and maximum AII-, ACTH-, and potassium-stimulated levels of corticosterone, 18-OHB and aldosterone also were similar in the cells from SHR and WKY. alpha-hANP similarly inhibited basal and stimulated levels of these corticosteroids in the cells from SHR and WKY. These results indicate that the inhibitory effect of alpha-hANP on aldosteronogenesis is unaltered in SHR at 14 weeks of age on a normal sodium diet.
Uchida
K
K
Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan.
Azukizawa
S
S
Kamei
M
M
Yoshida
I
I
Kigoshi
T
T
Yamamoto
I
I
Hosojima
H
H
Morimoto
S
S
D015069:18-Hydroxycorticosterone / Q000096:biosynthesis; D000302:Adrenal Cortex / Q000187:drug effects*; D000324:Adrenocorticotropic Hormone / Q000502:physiology; D000450:Aldosterone / Q000096:biosynthesis*; D000804:Angiotensin II / Q000502:physiology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D003345:Corticosterone / Q000096:biosynthesis; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012964:Sodium / Q000378:metabolism
D010446:Peptide Fragments; D000804:Angiotensin II; D000450:Aldosterone; D015069:18-Hydroxycorticosterone; D009320:Atrial Natriuretic Factor; D000324:Adrenocorticotropic Hormone; D012964:Sodium; D011188:Potassium; D003345:Corticosterone