Determination of association constants of beta-cyclodextrin and beta-cyclodextrin-bearing polymers with drugs by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) support was prepared, based on silica beads coated with a beta-cyclodextrin-containing polymer, which allows the elution of solutes in order of their affinity for beta-cyclodextrin. Binding constants were determined from the retention data for drugs eluted with pure buffer on the new support and good agreement was observed with results obtained by the Hummel and Dreyer method, previously used in HPLC studies of drug-protein interactions.
Sébille
B
B
Laboratoire de Physico-Chimie des Biopolymères, Unité Mixte No. 27, CNRS, Université Paris XII, Thiais, France.
Thuaud
N
N
Piquion
J
J
Behar
N
N
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000032:analysis*; D003912:Dextrins / Q000032:analysis*; D007202:Indicators and Reagents; D007536:Isomerism; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011108:Polymers / Q000032:analysis; D012822:Silicon Dioxide; D013213:Starch / Q000032:analysis*; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D003912:Dextrins; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D011108:Polymers; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide; D013213:Starch; C031215:betadex