Detection of vesicular stomatitis virus (VSV) RNA in the central nervous system of infected mice by in situ hybridization.
Using in situ hybridization with a cloned DNA probe specific for the VSV G protein, viral RNA was detected and localized in CNS tissue of mice infected i.c. with either wild or ts G 31 VSV mutant. In both cases, brain and spinal cord neurons were the only cells seen to contain viral RNA. Virus-positive neurons were observed enclosed in spongious areas induced by the ts VSV mutant. These results suggest that the VSV shows a strong tropism for the neuronal cell and indicate that the vacuole formation might be associated with the expression of the VSV G protein gene in infected neurons.
Fournier
J G
JG
Unité 43, Institut National de la Santé et de la Recherche Médicale, Hôpital Saint Vincent de Paul, Paris, France.
Robain
O
O
Cerutti
I
I
Tardivel
I
I
Chany-Fournier
F
F
Chany
C
C
D000818:Animals; D002490:Central Nervous System / Q000032:analysis* / Q000382:microbiology; D004679:Encephalomyelitis / Q000382:microbiology*; D008562:Membrane Glycoproteins; D051379:Mice; D009474:Neurons / Q000032:analysis / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000302:isolation & purification*; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins / Q000032:analysis / Q000235:genetics
C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins