title: Estrogen-dependent in vitro transcription from the vitellogenin promoter in liver nuclear extracts.
abstract: One approach to analyzing the molecular mechanisms of gene expression in vivo is to reconstitute these events in cell-free systems in vitro. Although there is some evidence for tissue-specific transcription in vitro, transcriptionally active extracts that mimic a steroid hormone-dependent enhancement of transcription have not been described. In the study reported here, nuclear extracts of liver from the frog Xenopus laevis were capable of estrogen-dependent induction of a homologous vitellogenin promoter that contained the estrogen-responsive element.
authors.affiliation: Institut de Biologie animale, Université de Lausanne, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Corthésy
authors.forename: R
authors.initials: R
authors.lastname: Hipskind
authors.forename: I
authors.initials: I
authors.lastname: Theulaz
authors.forename: W
authors.initials: W
authors.lastname: Wahli
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D015500:Chloramphenicol O-Acetyltransferase; D004274:DNA, Recombinant; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects*; D014819:Vitellogenins / Q000235:genetics*; D014982:Xenopus laevis
chemical_list: D004274:DNA, Recombinant; D014819:Vitellogenins; D004958:Estradiol; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D012319:RNA Polymerase II