Bradykinin stimulation of inositol phosphate and calcium responses in insensitive to pertussis toxin in NG115-401L neuronal cells.
Stimulation of NG115-401L neuronal cells with bradykinin produces a dose-dependent increase in inositol phosphate production which is not blocked, rather slightly increased, after treatment with pertussis toxin. Nevertheless, pertussis toxin stimulates ADP-ribosylation of a 41K membrane protein, and blocks opioid receptor-mediated inhibition of stimulated cAMP production in these cells. These results suggest that bradykinin responses in the NG115-401L cells are pertussis-insensitive, unlike bradykinin responses reported in other neuronal cell lines.
Jackson
T R
TR
MRC Molecular Neurobiology Unit, University of Cambridge Medical School, UK.
Patterson
S I
SI
Wong
Y H
YH
Hanley
M R
MR
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D005910:Glioma; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000096:biosynthesis*; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D037342:Pertussis Toxin; D051381:Rats; D011957:Receptors, Opioid / Q000502:physiology; D013403:Sugar Phosphates / Q000096:biosynthesis*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D007295:Inositol Phosphates; D008565:Membrane Proteins; D011957:Receptors, Opioid; D013403:Sugar Phosphates; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D007294:Inositol; D000242:Cyclic AMP; D037342:Pertussis Toxin; D000527:Alprostadil; D001920:Bradykinin