title: Oxidation of cytochrome c2 and of cytochrome c by reaction centers of Rhodospirillum rubrum and Rhodobacter sphaeroides. The effect of ionic strength and of lysine modification on oxidation rates.
abstract: The oxidation of cytochrome c2 by the photooxidized reaction center bacteriochlorophyll, P+-870, in chromatophores of Rhodospirillum rubrum can be described using second-order kinetics at all ionic strengths. In a system consisting of isolated R. rubrum reaction centers and purified R. rubrum cytochrome c2, the oxidation of cytochrome c2 also follows second-order kinetics. In both cases, the reaction rates at low ionic strength are weakly dependent on the ionic strength. The data suggest that the cytochrome remains mobile at very low ionic strength, since the observed kinetics can be easily explained assuming no significant tight binding of cytochrome c2 to the reaction center. In a system consisting of equine cytochrome c and reaction centers of either R. rubrum or Rhodobacter sphaeroides, the cytochrome c oxidation rate depends more strongly on the ionic strength. The high reaction rates at low ionic strength suggest that a significant portion of the cytochrome is bound. Using equine cytochrome c derivatives modified at specific lysine residues, it was shown that both R. rubrum and Rb. sphaeroides reaction centers react with equine cytochrome c through its exposed heme edge.
authors.affiliation: Department of Biophysics, Huygens Laboratory of the State University, Leiden, The Netherlands.
authors.forename: H N
authors.initials: HN
authors.lastname: van der Wal
authors.forename: R
authors.initials: R
authors.lastname: van Grondelle
authors.forename: F
authors.initials: F
authors.lastname: Millett
authors.forename: D B
authors.initials: DB
authors.lastname: Knaff
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D045362:Cytochromes c2; D004579:Electron Transport; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes; D008239:Lysine; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D045322:Photosynthetic Reaction Center Complex Proteins; D012247:Rhodospirillum rubrum / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045362:Cytochromes c2; D008239:Lysine