title: Monocyte-derived macrophages as helper cells in monocyte-mediated cytolysis.
abstract: Human peripheral blood monocytes (M), incubated with opsonized zymosan particles (OPZ), lysed human erythrocyte (RBC) targets, as detected by a 51Cr release method. Conversely, cells derived in vitro from M (monocyte-derived macrophages, MDM) were ineffective. When added to the M-RBC system, MDM enhanced the lysis. The lysis by M and M plus MDM was prevented by catalase, azide and amino acids (alanine, taurine), consistent with the requirement for hypochlorous acid (HOCl). Moreover, MDM per se incapable of generating HOCl augmented the HOCl recovery from the M-RBC system. The results provide evidence for a previously unrecognized form of interaction between two distinct populations of mononuclear phagocytes.
authors.affiliation: First Medical Clinic, University of Genova Medical School, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Dallegri
authors.forename: A
authors.initials: A
authors.lastname: Ballestrero
authors.forename: G
authors.initials: G
authors.lastname: Frumento
authors.forename: R
authors.initials: R
authors.lastname: Goretti
authors.forename: F
authors.initials: F
authors.lastname: Patrone
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology*; D009895:Opsonin Proteins / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D001386:Azides; D009895:Opsonin Proteins; D006997:Hypochlorous Acid; D015054:Zymosan; D002374:Catalase