Hyperparathyroidism is associated with reduced expression of a parathyroid calcium receptor mechanism defined by monoclonal antiparathyroid antibodies.
Parathyroid tissue from patients with hyperparathyroidism (HPT) exhibited reduced immunohistochemical reactivity with monoclonal antiparathyroid antibodies, previously shown to stain intensely the surface of normal human parathyroid cells and to interfere with a receptor mechanism of these cells which is involved in the sensing and gating of Ca2+. Parathyroid hormone (PTH) release and cytoplasmic Ca2+ concentrations (Ca2+i) of dispersed cells from the pathological parathyroid glands had right-shifted dependencies on extracellular Ca2+, and exposure to the antibodies rendered both Ca2+i and PTH release almost completely insensitive to changes in ambient Ca2+. The results suggest that reduced expression of a parathyroid calcium receptor mechanism may be an important cause for the aberrant PTH release in HPT.
Juhlin
C
C
Department of Surgery, University of Uppsala, Sweden.
Klareskog
L
L
Nygren
P
P
Ljunghall
S
S
Gylfe
E
E
Rastad
J
J
Akerström
G
G
D000236:Adenoma / Q000378:metabolism; D000911:Antibodies, Monoclonal; D002118:Calcium / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D006961:Hyperparathyroidism / Q000378:metabolism*; D006965:Hyperplasia; D007124:Immunoenzyme Techniques; D010280:Parathyroid Glands / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D010281:Parathyroid Hormone / Q000378:metabolism; D010282:Parathyroid Neoplasms / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D010281:Parathyroid Hormone; D011956:Receptors, Cell Surface; D002118:Calcium