title: Proton nuclear magnetic resonance studies of mast cell histamine.
abstract: The state of histamine in mast cells was studied by 1H NMR spectroscopy. Spectra were measured for histamine in situ in intact mast cells, for histamine in suspensions of mast cell granule matrices that had been stripped of their membranes, and for histamine in solutions of heparin. The 1H NMR spectrum of intact mast cells is relatively simple, consisting predominantly of resonances for intracellular histamine superimposed on a weaker background of resonances from heparin and proteins of the cells. All of the intracellular histamine contributes to the NMR signals, indicating it must be relatively mobile and not rigidly associated with the negatively charged granule matrix. Spectra for intracellular histamine and for histamine in granule matrices are similar, indicating the latter to be a reasonable model for the in situ situation. The dynamics of binding of histamine by granule matrices and by heparin are considerably different; exchange of histamine between the bulk water and the granule matrices is slow on the 1H NMR time scale, whereas exchange between the free and bound forms in heparin solution is fast. The chemical shifts of resonances for histamine in mast cells are pH dependent, decreasing as the intragranule pH increases without splitting or broadening. The results are interpreted to indicate that histamine in mast cells is relatively labile, with rapid exchange between bound histamine and pools of free histamine in water compartments confined in the granule matrix.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Canada.
authors.forename: D L
authors.initials: DL
authors.lastname: Rabenstein
authors.forename: R
authors.initials: R
authors.lastname: Ludowyke
authors.forename: D
authors.initials: D
authors.lastname: Lagunoff
mesh_terms: D000818:Animals; D003903:Deuterium; D017666:Deuterium Oxide; D006493:Heparin; D006632:Histamine / Q000032:analysis*; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008407:Mast Cells / Q000032:analysis*; D051381:Rats; D014867:Water
chemical_list: D014867:Water; D006859:Hydrogen; D006632:Histamine; D006493:Heparin; D003903:Deuterium; D017666:Deuterium Oxide