Mitochondrial plasmid DNAs of broad bean: nucleotide sequences, complex secondary structures, and transcription.
Three circular plasmid DNA molecules of 1704, 1695 and 1476 nucleotide pairs from broad bean mitochondria (mt-plasmids 1-3) have been sequenced. Within a highly homologous segment of mt-plasmid 1 and 2 are found a series of six directly repeated, inverted repeat sequences, separated by unique sequences. Mt-plasmid 3 contains a series of four inverted repeat sequences, unrelated to the inverted repeat sequences of mt-plasmids 1 and 2. Two RNA molecules of about 440 and 320 nucleotides that are complementary to mt-plasmid 2 were detected. Mapping of 5' and 3' termini of these complementary RNA molecules indicated that all transcription from mt-plasmid 2 occurs within a 441 nucleotide region of the molecule. Evidence for transcription of mt-plasmids 1 and 3 was not found.
Wahleithner
J A
JA
Department of Biology, University of Utah, Salt Lake City 84112.
Wolstenholme
D R
DR
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D007887:Fabaceae / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal; D010957:Plasmids; D014158:Transcription, Genetic
D004272:DNA, Mitochondrial; D004262:DNA Restriction Enzymes