title: Comparison of the leader sequences of four group A streptococcal M protein genes.
abstract: The 5' portions and flanking sequences of genes encoding types 1, 12, 24, and 6 M proteins were compared. Although the DNA sequences encoding the amino-termini of the mature M proteins had no obvious similarity, upstream sequences, and those encoding the signal peptides (leader sequences) of the four M protein genes had considerable similarity. In general, the 5' ends of all the leader sequences were more conserved than the 3' ends, although the M6 and M24 leader sequences had identical 3' ends. Sequence similarity among the deduced amino acid sequences of the four signal peptides was more extensive than the corresponding DNA sequences. We found that strict DNA similarity among all four sequences extended only to the ends of the hydrophilic amino-terminal regions of the signal peptides, but that amino acid sequence conservation continued to the ends of the respective hydrophobic cores. With the exception of the M6 and M24 sequences, the regions adjacent to the signal peptidase cleavage sites were highly variable.
authors.affiliation: Department of Microbiology, University of Minnesota, Minneapolis 55455.
authors.forename: E
authors.initials: E
authors.lastname: Haanes-Fritz
authors.forename: W
authors.initials: W
authors.lastname: Kraus
authors.forename: V
authors.initials: V
authors.lastname: Burdett
authors.forename: J B
authors.initials: JB
authors.lastname: Dale
authors.forename: E H
authors.initials: EH
authors.lastname: Beachey
authors.forename: P
authors.initials: P
authors.lastname: Cleary
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D021382:Protein Sorting Signals / Q000235:genetics*; D013297:Streptococcus pyogenes / Q000235:genetics*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D021382:Protein Sorting Signals; C014689:streptococcal M protein; D004262:DNA Restriction Enzymes