12-O-tetradecanoylphorbol-13-acetate induces the enhancer function of human T-cell leukemia virus type I.
Phorbol esters were employed in studies on the molecular mechanism of the induction of expression of human T-cell leukemia virus type I (HTLV-I) by a tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA). Experiments using the chloramphenicol acetyltransferase (CAT) system showed that CAT expression directed by the long terminal repeat (LTR) of HTLV-I was induced by TPA, but not by 4 alpha-phorbol-12,13-didecanoate, which is not an activator of protein kinase C, and that like other known enhancers, irrespective of its position and orientation, a 230-bp fragment in the U3 region of the HTLV-I LTR confers susceptibility to induction by TPA.
Fujii
M
M
Institute for Virus Research, Kyoto University, Japan.
Nakamura
M
M
Ohtani
K
K
Sugamura
K
K
Hinuma
Y
Y
D017977:Deltaretrovirus / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006801:Humans; D007938:Leukemia / Q000382:microbiology*; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000382:microbiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D013755:Tetradecanoylphorbol Acetate