Multiple insertions and tandem repeats of origin-minus simian virus 40 DNA in transformed rat and mouse cells.
Stable simian virus 40 (SV40) transformation requires integration and expression of the early region of the SV40 genome. We have examined the amount and state of integrated viral DNA of SV40-transformed NIH 3T3 mouse and F2408 rat fibroblast lines generated by transfection with either wild-type or origin-defective SV40 DNA. A functional SV40 replication origin was not required for multiple inserts and partial-repeat structures to form in NIH 3T3 mouse transformants. In contrast, partial repeats in F2408 rat transformants were rare when the SV40 replication origin was intact and not detected at all when it was defective.
Blanck
G
G
Department of Biological Sciences, Columbia University, New York, New York 10027.
Li
D
D
Pomert
E
E
Pollack
R
R
Chen
S
S
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D005347:Fibroblasts / Q000382:microbiology; D051379:Mice; D010957:Plasmids; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D004251:DNA Transposable Elements; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI