title: Chromosome replication in Escherichia coli induced by oversupply of DnaA.
abstract: Overexpression of DnaA protein from a multicopy plasmid accompanied by a shift to 42 degrees C causes initiation of one extra round of replication in a dnaA+ strain grown in glycerol minimal medium. This extra round of replication does not lead to an extra cell division, such that cells contain twice the normal number of chromosomes.
authors.affiliation: Biology Programs, University of Texas at Dallas, Richardson 75080.
authors.forename: Y C
authors.initials: YC
authors.lastname: Xu
authors.forename: H
authors.initials: H
authors.lastname: Bremer
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D002455:Cell Division; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial