title: Lithium transport in human fibroblasts: relationship to RBC lithium transport and psychiatric diagnoses.
abstract: Cultured fibroblasts were prepared from six normal controls, five DSM-III manic patients, and six DSM-III schizophrenic patients. Lithium (Li+) uptake, 24-hour Li+ ratios, and steady-state membrane potential were measured in these cell lines. The uptake of 10 mM Li+ reached maximum at 2 hours, with an intracellular concentration of approximately 15 mM. No significant difference in uptake was found among subject groups. Twenty-four hour Li+ (ratio of intracellular/extracellular Li+) ratios were determined by incubating the cell lines for 24 hours in the presence of 2 mM Li+. No significant difference was observed among groups; nor was there any significant correlation between the fibroblast 24-hour ratios and 24-hour in vitro ratios determined in donor red cells. The relationship between membrane potential and the 24 hour Li+ ratio in fibroblasts was determined. The average potential in these cell lines was -56 mV and was not affected by Li+ treatment. No correlation between the Li+ ratio and membrane potential was found.
authors.affiliation: Department of Psychiatry, S.U.N.Y., Stony Brook 11794-8101.
authors.forename: R
authors.initials: R
authors.lastname: Hitzemann
authors.forename: L
authors.initials: L
authors.lastname: Kao
authors.forename: J
authors.initials: J
authors.lastname: Hirschowitz
authors.forename: D
authors.initials: D
authors.lastname: Garver
authors.forename: E
authors.initials: E
authors.lastname: Gruenstein
mesh_terms: D000328:Adult; D001714:Bipolar Disorder / Q000097:blood*; D002712:Chlorides / Q000493:pharmacokinetics*; D002940:Circadian Rhythm; D004912:Erythrocytes / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D008094:Lithium / Q000493:pharmacokinetics*; D018021:Lithium Chloride; D008564:Membrane Potentials; D012559:Schizophrenia / Q000097:blood*
chemical_list: D002712:Chlorides; D008094:Lithium; D018021:Lithium Chloride