Parallel determination of mineralocorticoid and glucocorticoid receptors in T- and B-lymphocytes of human spleen.
The binding characteristics of glucocorticoid and mineralocorticoid receptors were determined in T- and B-lymphocytes from the spleen of four deceased kidney donors. The number of glucocorticoid and mineralocorticoid receptors was equivalent in T- and B-cells (respectively, 1308 +/- 364 and 1335 +/- 520 glucocorticoid receptors per cell, and 174 +/- 29 and 164 +/- 24 mineralocorticoid receptors per cell). The number of binding sites per cell was 5- to 10-fold higher for dexamethasone than for aldosterone. The apparent dissociation constant of dexamethasone for glucocorticoid receptors was 2-fold higher than that of aldosterone for mineralocorticoid receptors. In conclusion, subpopulations of human lymphocytes have an equal number of glucocorticoid and mineralocorticoid receptors.
Armanini
D
D
Medizinische Klinik Innenstadt and Kinderklinik, University of Munich, FRG.
Endres
S
S
Kuhnle
U
U
Weber
P C
PC
D001402:B-Lymphocytes / Q000378:metabolism*; D006801:Humans; D011869:Radioligand Assay; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid; C028697:dexamethasone receptor