Standard heparin enhances the antithrombotic activity of dermatan sulfate in the rabbit but CY 216 does not.
Standard heparin (SH) and dermatan sulfate (DS) two glycosaminoglycans with different pharmacological targets are effective antithrombotic agents in the rabbit. We have investigated the antithrombotic activity of the association DS plus SH. It was found that doses as low as 25 micrograms/kg for DS and 10 micrograms/kg for SH were ineffective when injected separately but generated a high and significant antithrombotic activity when injected together. These results were confirmed when higher doses of each compound were delivered in association. Further experiments were performed to determine if the enhancement of the antithrombotic activity of DS by HS resulted from its anti-factor IIa or anti-factor Xa activity or from its moiety without affinity to AT III. A low molecular weight heparin (CY 216) with an anti-factor Xa/anti-factor IIa ratio of 5, the synthetic pentasaccharide bearing the minimum binding sequence to antithrombin III, and a low affinity fraction of SH to AT III did not increase the antithrombotic activity of DS; in contrast a high affinity fraction of SH to AT III had the same effect than SH. We conclude that the enhancement of the antithrombotic activity of DS by SH mainly results from its anti-factor IIa activity.
Cadroy
Y
Y
Laboratoire d'Hemostase, Centre de Transfusion Sanguine, Toulouse, France.
Dol
F
F
Caranobe
C
C
Petitou
M
M
Lormeau
J C
JC
Sié
P
P
Choay
J
J
Boneu
B
B
D000818:Animals; D002807:Chondroitin / Q000031:analogs & derivatives*; D003871:Dermatan Sulfate / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D015951:Factor Xa; D005343:Fibrinolytic Agents / Q000008:administration & dosage*; D006493:Heparin / Q000008:administration & dosage*; D006495:Heparin, Low-Molecular-Weight / Q000008:administration & dosage*; D008297:Male; D011516:Prothrombin / Q000037:antagonists & inhibitors; D011817:Rabbits; D015842:Serine Proteinase Inhibitors; D013927:Thrombosis / Q000188:drug therapy
D005343:Fibrinolytic Agents; D006495:Heparin, Low-Molecular-Weight; D015842:Serine Proteinase Inhibitors; D003871:Dermatan Sulfate; D011516:Prothrombin; C025337:Factor IIa; D006493:Heparin; D002807:Chondroitin; D015951:Factor Xa