Lipid peroxidation and lipid peroxide detected by chemiluminescence.
This article emphasizes the advantages of using a luminescence spectrometer based on photon counting techniques for the detection of lipid peroxidation. An overview is presented of how chemiluminescence can be stimulated in the luminol-cytochrome c heme peptide system as an assay for lipid hydroperoxides. This method is used for finding antioxidant drugs. The specificity and advantages of the chemiluminescent method for detecting lipid hydroperoxides is reviewed.
Iwaoka
T
T
Analytical and Metabolic Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Tabata
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F
Takahashi
T
T
D000818:Animals; D003574:Cytochrome c Group / Q000032:analysis; D006418:Heme / Q000032:analysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D008054:Lipid Peroxides / Q000032:analysis* / Q000378:metabolism; D008163:Luminescent Measurements; D010455:Peptides / Q000032:analysis
D003574:Cytochrome c Group; D008054:Lipid Peroxides; D010455:Peptides; D006418:Heme; D006861:Hydrogen Peroxide