title: Replication and cytopathic effects of ovine lentivirus strains in alveolar macrophages correlate with in vivo pathogenicity.
abstract: Ruminant lentiviruses share genomic sequences and biologic properties with human immunodeficiency viruses. Four ovine lentivirus strains were assessed for cytopathic effects and virus replication. Lentivirus isolate H/24 produced high virus titers and lysis of synovial cells but replicated slowly and caused no fusion of alveolar macrophages. Lentivirus isolates 84/28 and 85/14 produced low virus titers, less syncytia, and limited or no cell lysis in synovial cells and macrophages. In contrast, ovine lentivirus isolate 85/34 produced early peak virus titers and caused rapid fusion and lysis of both macrophages and synovial cells. Ovine lentivirus isolates which were cytopathic for macrophages induced lymphoproliferative disease when inoculated into lambs.
authors.affiliation: Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
authors.forename: M D
authors.initials: MD
authors.lastname: Lairmore
authors.forename: G Y
authors.initials: GY
authors.lastname: Akita
authors.forename: H I
authors.initials: HI
authors.lastname: Russell
authors.forename: J C
authors.initials: JC
authors.lastname: DeMartini
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D008264:Macrophages / Q000382:microbiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000382:microbiology*; D012756:Sheep; D013583:Synovial Membrane / Q000382:microbiology; D014779:Virus Replication; D014790:Visna-maedi virus / Q000472:pathogenicity* / Q000502:physiology / Q000648:ultrastructure