Thermodynamics of B-Z transition of poly[d(G-C)] in a water-ethanol solution. The "tie calorimetry".
Poly[d(G-C)] in a 55% ethanol solution undergoes a transition from the Z form to the B form when the temperature is increased from 20 degrees to 50 degrees C. The enthalpy of the transition, delta HBA = -1.4 kcal/mol, has been determined with a "tie" polyamine which stabilizes the Z conformation. This value has been shown to be practically independent of ionic strength within the range of 5 X 10(-4) M - 2 X 10(-3) M NaCl.
Karapetyan
A T
AT
Kirovakan Teachers Training Institute, Armenian SSR, USSR.
Minyat
E E
EE
Vardevanyan
P O
PO
Ivanov
V I
VI
D002151:Calorimetry; D004247:DNA; D000431:Ethanol; D011089:Polydeoxyribonucleotides; D012996:Solutions; D013816:Thermodynamics; D014867:Water
D011089:Polydeoxyribonucleotides; D012996:Solutions; D014867:Water; C016681:poly(dG).poly(dC); D000431:Ethanol; D004247:DNA