T cells subsets responsible for clearance of Sendai virus from infected mouse lungs.
T cell subsets responsible for clearance of Sendai virus from mouse lungs determined by adoptive transfer of immune spleen cell fractions to infected nude mice. T cells with antiviral activity developed in spleens by 7 days after intranasal infection. Spleen cell fractions depleted of Lyt-2+, Lyt-1+, or L3T4+ cells showed antiviral activity in vivo, although the degree of the activity was lower than that of control whole spleen cells. The antiviral activity of the Lyt-2+ cell-depleted fraction was consistently higher than that of L3T4+ (Lyt-1+)-depleted cells. In vitro cytotoxic activity against Sendai virus-associated, syngeneic lipopolysaccharide-blast cells was detected in stimulated cells from intraperitoneally immunized mice but was lost after depletion of Lyt-2+ cells. Multiple injection of anti-Sendai virus antibody into infected nude mice had no effect on lung virus titer. These results indicate that L3T4+ (Lyt-1+) and Lyt-2+ subsets are cooperatively responsible for efficient clearance of Sendai virus from the mouse lung.
Iwai
H
H
Department of Veterinary Public Health, Institute of Public Health, Minato-ku, Tokyo.
Machii
K
K
Otsuka
Y
Y
Ueda
K
K
D000818:Animals; D003602:Cytotoxicity, Immunologic; D005260:Female; D007116:Immunization, Passive; D008168:Lung / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D012016:Reference Values; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology