Effect of PGE2 and LTB4 on vicia villosa binding lymphocytes.
Since there is a good deal of evidence that vicia villosa lectin (VVA) binds to contrasuppressor cells, mouse splenocytes and thymocytes were sorted by binding to VVA-coated Petri dishes. It was observed that vicia villosa adherent cells (VVA(+)) did not proliferate when mitogens were added to cultures, but they did enhance the thymidine uptake of PHA or ConA stimulated vicia villosa non-adherent (VVA(-)) splenocytes. PGE2 treatment of VVA(+) splenocytes or VVA(+) immature thymocytes did not very much affect the VVA(+) cell behavior. On the other hand, LTB4 increased the enhancing capability of VVA(+) splenocytes. Therefore, investigations dealing with the effects of LTB4 on lymphocyte subsets which may include VVA(+) cells should take into consideration the possible presence of contrasuppressor cells.
Gualde
N
N
Groupe de Recherche en Immunologie et Biochimie, Faculté de Médecine et de Pharmacie, Limoges, France.
Cook
J M
JM
Guibert
F
F
D000818:Animals; D015232:Dinoprostone; D005260:Female; D037102:Lectins / Q000378:metabolism*; D007975:Leukotriene B4 / Q000031:analogs & derivatives*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000145:classification / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins; D011458:Prostaglandins E / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
D037102:Lectins; D037121:Plant Lectins; D011458:Prostaglandins E; C042242:Vicia lectins; D007975:Leukotriene B4; D015232:Dinoprostone