Separation of simian virus 40 large-T-antigen-transforming and origin-binding functions from the ability to block differentiation.
Wild-type simian virus 40 large T antigen is very effective at blocking adipocyte differentiation in 3T3-F442A cells as assayed by triglyceride accumulation, induction of glycerophosphate dehydrogenase activity, and expression of mRNAs for glycerophosphate dehydrogenase, the adipocyte serine protease adipsin, and the putative lipid-binding protein adipocyte P2. Point mutants defective for either origin-specific DNA binding or transformation blocked differentiation as completely as wild type.
Cherington
V
V
Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Brown
M
M
Paucha
E
E
St Louis
J
J
Spiegelman
B M
BM
Roberts
T M
TM
D000273:Adipose Tissue / Q000166:cytology; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000276:immunology*; D002454:Cell Differentiation; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D007118:Immunoassay; D009154:Mutation; D009857:Oncogenes; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology*
D000952:Antigens, Polyomavirus Transforming