title: Differences in the solution structures of oxidized and reduced cytochrome c measured by small-angle X-ray scattering.
abstract: While X-ray crystallographic data on cytochrome c show the reduced and oxidized forms to have very similar structures, there is a considerable body of data, mostly from solution studies, that indicates the reduced form is more stable and that the interior of the protein is less accessible to solvent in this state. These observations have led to the hypothesis that while the time-averaged structure is preserved between the two forms, the dynamics of the two forms are different. The oxidized form has been proposed to undergo more large-amplitude, low-frequency motions than the reduced form. The crystal structure data were derived from crystals grown in high salt concentrations, but the solution studies were done at relatively low ionic strength. Small-angle X-ray scattering has been used to examine the effects of the ionic strength and oxidation state on the solution structure of cytochrome c. We find that the radius of gyration and the maximum linear dimension of oxidized cytochrome c are significantly larger than those for reduced cytochrome c, in 5 mM phosphate buffer at pH 7.3, and further that this difference is suppressed by addition of 200 mM sodium chloride. We conclude that there is a real structural difference between the two forms at low ionic strength in solution and that this difference is likely to contribute to the observed differences in accessibility and compressibility.
authors.affiliation: Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
authors.forename: J
authors.initials: J
authors.lastname: Trewhella
authors.forename: V A
authors.initials: VA
authors.lastname: Carlson
authors.forename: E H
authors.initials: EH
authors.lastname: Curtis
authors.forename: D B
authors.initials: DB
authors.lastname: Heidorn
mesh_terms: D003574:Cytochrome c Group / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D011487:Protein Conformation; D012996:Solutions; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D003574:Cytochrome c Group; D012996:Solutions