Antigen-specific immune-suppressor factor in herpes simplex virus type 2 infections of UV B-irradiated mice.
UV B-irradiation (280 to 320 nm) of mice at the site of cutaneous infection with herpes simplex virus type 2 (HSV-2) induced suppressor T-cell circuits that decreased HSV-2-induced proliferative responses of HSV-2-immune lymph node cells. Adoptive transfer experiments indicated that splenocytes from UV B-irradiated HSV-2-infected animals contain L3T4+ cells that suppress proliferative responses in vivo, consistent with suppressor inducer cells. However, following in vitro culture of the splenocytes with HSV-2 antigen, the proliferation of immune lymph node cells was inhibited by Lyt2+ suppressor T cells, consistent with antigen-induced suppressor effector cells. Antigen-specific and nonspecific suppressor factors were fractionated from supernatants of HSV-2-stimulated spleen cells by molecular-sieve chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the Sephadex fraction that contained the antigen-specific suppressor factor, in the presence or absence of 2-mercaptoethanol, defined a 115-kilodalton protein consisting of two disulfide-bound components with molecular sizes of 70 and 52 kilodaltons. The implications of these results with respect to the regulation of HSV-induced cell-mediated immunity following UV B-irradiation are discussed.
Aurelian
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Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
Yasumoto
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Smith
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D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D005260:Female; D006561:Herpes Simplex / Q000276:immunology* / Q000473:pathology; D007111:Immunity, Cellular / Q000528:radiation effects; D007116:Immunization, Passive; D008198:Lymph Nodes / Q000473:pathology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology*; D012874:Skin Diseases, Infectious / Q000276:immunology* / Q000473:pathology; D013491:Suppressor Factors, Immunologic / Q000302:isolation & purification; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000637:transplantation; D014466:Ultraviolet Rays
D000956:Antigens, Viral; D013491:Suppressor Factors, Immunologic