title: Molecular cloning of an endogenous cat retroviral element (ECE 1)--a recombinant between RD-114 and FeLV-related sequences. Brief report.
abstract: A lambda L 47.1 clone library was constructed from BamHI digests of cellular cat DNA and was screened with cDNA probes specific for the 3'-terminus of the replication competent RD-114 virus. The first analysis of one clone (lambda-ECE 1) shows that it consists at least of a 3'-LTR and a 5' adjacent env gene. The LTR nucleotide sequences of the RD-114 cDNA clones and ECE 1 are identical within the R region and unrelated to FeLV. Otherwise, the region upstream from the 3'-LTR of ECE 1 shows homology to the transmembrane protein encoding gene of FeLV, but not to RD-114. These results imply that ECE 1 is a recombinant between an RD-114-like 3'-LTR and a FeLV-related env portion.
authors.affiliation: Department of Cell Differentiation, Academy of Sciences of the German Democratic Republic, Berlin.
authors.forename: W
authors.initials: W
authors.lastname: Beyer
authors.forename: R
authors.initials: R
authors.lastname: Möhring
authors.forename: B
authors.initials: B
authors.lastname: Drescher
authors.forename: U
authors.initials: U
authors.lastname: Nötzel
authors.forename: S
authors.initials: S
authors.lastname: Rosenthal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000382:microbiology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005256:Leukemia Virus, Feline / Q000235:genetics; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004247:DNA