Isolation and characterization of cloned cDNAs that code for human ribosomal protein S6.
Ribosomal protein (rp) S6 is the major substrate of protein kinases in eukaryotic ribosomes. To facilitate the identification of cloned cDNAs for human rpS6, we used published amino acid (aa) sequence data for rat liver rpS6 and yeast (Saccharomyces carlsbergensis) rpS10 to design mixed oligodeoxynucleotide probes. Screening of several human cDNA libraries with these probes permitted the isolation of plasmids which encompass the entire coding sequence of rpS6 (249 aa residues), 27 bp of the 5'-untranslated leader and all 39 bp of the 3'-untranslated region. A comparison of the predicted human rpS6 amino acid sequence and the yeast rpS10 amino acid sequence shows highly conserved areas separated by regions of divergence.
Lott
J B
JB
Department of Biochemistry, Faculty of Medicine, University of Western Ontario, London, Canada.
Mackie
G A
GA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins; D004247:DNA; D004262:DNA Restriction Enzymes