title: Regulation of alcR, the positive regulatory gene of the ethanol utilization regulon of Aspergillus nidulans.
abstract: The alcR positive control gene is necessary for the expression of both alcA (coding for alcohol dehydrogenase ADH I), and aldA (coding for aldehyde dehydrogenase, AldDH) in Aspergillus nidulans. Using a cloned alcR probe and Northern blots analysis we show that: (1) alcR itself is inducible; (2) alcR inducibility depends on the expression of the alcR gene itself; and (3) alcR is subject to carbon catabolite repression and its expression is controlled by the negatively acting creA wide specificity gene. The repression of alcR is sufficient to explain the carbon catabolite repression of ADH I and AldDH.
authors.affiliation: Institut de Microbiologie, Université Paris-Sud, Orsay, France.
authors.forename: R
authors.initials: R
authors.lastname: Lockington
authors.forename: C
authors.initials: C
authors.lastname: Scazzocchio
authors.forename: D
authors.initials: D
authors.lastname: Sequeval
authors.forename: M
authors.initials: M
authors.lastname: Mathieu
authors.forename: B
authors.initials: B
authors.lastname: Felenbok
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D004262:DNA Restriction Enzymes; D000431:Ethanol / Q000378:metabolism*; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014158:Transcription, Genetic
chemical_list: D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase; D004262:DNA Restriction Enzymes