A mouse renin promoter containing the conserved decanucleotide element binds the same B-cell factors as an authentic immunoglobulin heavy chain promoter.
A mouse renin-1 gene promoter fragment, normally inactive in B-cells, becomes a potent promoter in these cells after insertion of the highly conserved decanucleotide (dc/cd sequence) of immunoglobulin heavy and light chain promoters [(1987) EMBO J. 6, 1685-1690]. We observe retarded complexes of the same electrophoretic mobility when the cd-containing renin promoter fragment or an authentic immunoglobulin heavy chain promoter fragment is incubated with a nuclear extract from myeloma cells, suggesting that the renin promoter is activated due to its acquired ability to bind a B-cell-specific positive factor. No retarded complexes are observed with the original renin promoter fragment thus questioning the presence of a repressor as an explanation for its lack of activity in B-cells.
Ekker
M
M
Unité de Génétique et Biochimie du Développement, LA CNRS 361, Institut Pasteur, Paris, France.
Doyen
N
N
Leblond-Francillard
M
M
Rougeon
F
F
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001665:Binding Sites; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000235:genetics; D051379:Mice; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology; D011401:Promoter Regions, Genetic; D012083:Renin / Q000235:genetics* / Q000378:metabolism
D004251:DNA Transposable Elements; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D012083:Renin