Functional characterization of an androgen response element in the first intron of the C3(1) gene of prostatic binding protein.
We demonstrate that the 204 bp intronic gene fragment of C3(1), which has a specific in vitro affinity for the androgen receptor, is able to confer androgen responsiveness to a heterologous promoter. This characteristic is completely destroyed by a single G----T substitution, affecting a 5'-TGTTCT-3' element that closely resembles the consensus sequence of the glucocorticoid and progesterone response elements (GRE/PRE). In fact we could show that this androgen response element (ARE) also acts as a similarly weak GRE or PRE in T-47D cells.
Claessens
F
F
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Belgium.
Celis
L
L
Peeters
B
B
Heyns
W
W
Verhoeven
G
G
Rombauts
W
W
D000727:Androgen-Binding Protein / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015723:Gene Library; D005796:Genes; D007438:Introns; D008297:Male; D010957:Plasmids; D011401:Promoter Regions, Genetic; D060789:Prostatein; D011485:Protein Binding; D051381:Rats; D011944:Receptors, Androgen / Q000378:metabolism*; D015183:Restriction Mapping; D060765:Secretoglobins; D014162:Transfection; D014598:Uteroglobin; D060788:Mammaglobin B
D000727:Androgen-Binding Protein; D015500:Chloramphenicol O-Acetyltransferase; D060789:Prostatein; D011944:Receptors, Androgen; D060765:Secretoglobins; D014598:Uteroglobin; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B