Production and consumption of T cell growth factor by peripheral blood lymphocytes of cattle infected with Taenia saginata.
Calves were infected with 25,000 or 50,000 viable eggs of Taenia saginata. Larval numbers ranged between 2077 and 6005. During infection the animals developed leucocytosis, which was mainly due to lymphocytosis. An apparently positive correlation was observed between the lymphocytosis and the in vitro proliferative response of the lymphocytes to antigen prepared from proglottids. Maximal in vitro blast transformation of the cells stimulated with antigen occurred on Days 12 and 32 post-infection (p.i.). Specific antibodies to T. saginata were demonstrated on Day 14 p.i. At that time, the proliferative response of the cells paralleled the formation of specific antibodies, particularly of the IgG class. The stimulated cells produced a lymphokine showing interleukin 2 (IL 2)-like activity, since the addition of supernatant of such cells to IL 2-dependent concanavalin A (Con A)-blast cells supported the in vitro growth of the cells. In addition, peripheral blood lymphocytes (PBL) specific for T. saginata could be maintained in long-term cultures when they were cultured in medium containing supernatants of MLA-144 cell lines. The data presented in this study indicate that cells specific for T. saginata produced and consumed T cell growth factor (TCGF).
Huwer
M
M
Institut für Parasitologie und Tropenveterinärmedizin, Freie Universität Berlin, F.R.G.
Sanft
S
S
Ahmed
J
J
Hörchner
F
F
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000276:immunology; D003552:Cysticercus / Q000254:growth & development; D004797:Enzyme-Linked Immunosorbent Assay; D007376:Interleukin-2 / Q000096:biosynthesis*; D007958:Leukocyte Count / Q000662:veterinary; D008213:Lymphocyte Activation; D008218:Lymphocytosis / Q000209:etiology* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013621:Taenia / Q000276:immunology; D013622:Taeniasis / Q000097:blood / Q000150:complications / Q000276:immunology / Q000662:veterinary*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007376:Interleukin-2