title: Radiation inactivation analysis of fusion and hemolysis by vesicular stomatitis virus.
abstract: Radiation inactivation analysis was used to determine the size of the functional unit responsible for fusion of vesicular stomatitis virus (VSV) with cardiolipin or phosphatidylcholine-phosphatidylethanolamine (1:1) liposomes, and for VSV-induced hemolysis. When radiation-insensitive background values were subtracted, the calculated functional units for all three activities were similar, ranging from 866 to 957 kDa, equivalent to about 15 G protein molecules. This is in striking contrast to results of similar studies with influenza and Sendai viruses, in which the functional unit corresponded in size to a single fusion protein monomer, and suggests that VSV fusion may occur by a different mechanism.
authors.affiliation: Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854-5635.
authors.forename: K
authors.initials: K
authors.lastname: Bundo-Morita
authors.forename: S
authors.initials: S
authors.lastname: Gibson
authors.forename: J
authors.initials: J
authors.lastname: Lenard
mesh_terms: D006461:Hemolysis; D008081:Liposomes; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000502:physiology / Q000528:radiation effects*; D008970:Molecular Weight; D009975:Orthomyxoviridae / Q000528:radiation effects; D010222:Parainfluenza Virus 1, Human / Q000528:radiation effects; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology* / Q000528:radiation effects; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins / Q000502:physiology / Q000528:radiation effects*
chemical_list: C028391:G protein, vesicular stomatitis virus; D008081:Liposomes; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins