Synovial fluid inorganic pyrophosphate concentration and nucleotide pyrophosphohydrolase activity in basic calcium phosphate deposition arthropathy and Milwaukee shoulder syndrome.
Synovial fluid (SF) inorganic pyrophosphate (PPi) concentration is elevated in calcium pyrophosphate dihydrate (CPPD) crystal deposition arthropathy. Since CPPD and basic calcium phosphate (BCP) crystals often are present in the same joints, we determined [PPi] and activity of the PPi-generating enzyme, nucleotide pyrophosphohydrolase (NPPH), in SF from the joints of patients with various arthropathies, including those with BCP crystals. We found elevated SF [PPi] in joints with BCP crystals, as well as in joints with CPPD crystals. The presence of BCP crystals in synovial fluids was also predictive of elevated NPPH activity.
Rachow
J W
JW
Department of Medicine, Medical College of Wisconsin, Milwaukee 53226.
Ryan
L M
LM
McCarty
D J
DJ
Halverson
P C
PC
D002130:Calcium Phosphates / Q000378:metabolism*; D003460:Crystallization; D011756:Diphosphates / Q000378:metabolism*; D006801:Humans; D007592:Joint Diseases / Q000378:metabolism*; D009994:Osmolar Concentration; D010003:Osteoarthritis / Q000378:metabolism; D011309:Preservation, Biological; D011755:Pyrophosphatases / Q000378:metabolism; D012785:Shoulder Joint; D013577:Syndrome; D013582:Synovial Fluid / Q000201:enzymology / Q000378:metabolism*
D002130:Calcium Phosphates; D011756:Diphosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; D011755:Pyrophosphatases; C019757:ATP pyrophosphohydrolase; C485829:calcium phosphate, dibasic, anhydrous