FMRFamide receptors in Helix aspersa.
An in vitro receptor binding assay and an isolated heart bioassay were developed and used to characterize the structure-activity relations (SAR) of FMRFamide receptors in a land snail, Helix aspersa. In the radioreceptor assay, binding of 125I-desaminoTyr-PhenorLeu-Arg-Phe-amide (125I-daYFnLRFamide) at 0 degrees C to Helix brain membranes was reversible, saturable, and specific, with a KD of 14 nM and a Bmax of 85 fmol/mg brain. A lower affinity site was also observed (KD = 245 nM; Bmax = 575 fmol/mg brain). In the heart bioassay, daYFnLRFamide and other FMRFamide analogs increased myocardial contraction force. The SAR of cardiostimulation correlated with the specificity of high affinity 125I-daYFnLRFamide binding to brain and heart receptors. The SAR was also similar to that described for other molluscan FMRFamide bioassays, except for a marked preference for N-blocked analogs. Peptides with N-terminal extensions of desaminoTyr, Tyr, Tyr-Gly-Gly, and acetyl, exhibited the highest potency in both radioligand displacement and cardiostimulation. The endogenous Helix heptapeptide analogs of FLRFamide (pQDP-, NDP, and SDP-FLRFamide) were stimulatory on the heart at low doses, but were inhibitory at moderate to high doses. These peptides were 20 times weaker than FMRFamide in both the brain and heart receptor binding assays, with IC50s about 10 microM. The results suggest that the effects of FMRFamide in Helix are receptor-mediated, and that the heptapeptides do not interact at FMRFamide receptors.
Payza
K
K
Whitney Laboratory, University of Florida, St. Augustine 32086.
D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D019835:FMRFamide; D006372:Helix, Snails / Q000032:analysis*; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000032:analysis; D009479:Neuropeptides / Q000378:metabolism*; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000032:analysis*; D018010:Receptors, Invertebrate Peptide; D013329:Structure-Activity Relationship
C055209:FMRFamide receptor; D009479:Neuropeptides; D011956:Receptors, Cell Surface; D018010:Receptors, Invertebrate Peptide; D019835:FMRFamide