title: Do presynaptic opiate receptors and alpha-adrenoceptors alter acetylcholine release from a sympathetic ganglion by a similar mechanism?
abstract: The present experiments tested the possible involvement of a calcium-sensitive mechanism in the alpha-adrenoceptor- and opiate receptor-mediated inhibition of acetylcholine release from the cat superior cervical ganglion. First, the calcium-dependence of evoked acetylcholine release was measured in the presence and absence of the alpha-adrenoceptor agonist noradrenaline or of the opiate receptor agonist [Met5]enkephalin-Arg6-Phe7. When ganglia were perfused with Krebs medium containing [Ca2+] = 2.4, 1.2, 0.6, 0.2 mM, evoked release of acetylcholine was depressed by both agonists and the inhibition increased with reduced levels of extracellular Ca2+; this was especially evident when calcium in the medium was reduced to 0.2 mM. Second, the effects of both noradrenaline and [Met5]enkephalin-Arg6-Phe7 on calcium influx into presynaptic nerve endings was determined by measuring the accumulation of 45Ca into ganglia in the presence and absence of either drug. Both agonists reduced the stimulation-induced increase in 45Ca accumulation. The effect of noradrenaline to reduce calcium influx was blocked by yohimbine or by phentolamine; the effect of [Met5]enkephalin-Arg6-Phe7 to decrease 45Ca accumulation by ganglia was blocked by naloxone. It is concluded that activation of presynaptic opiate receptors and alpha-adrenoceptors in the cat superior cervical ganglion can alter acetylcholine release by a similar mechanism, i.e. to reduce Ca2+ influx during preganglionic nerve stimulation.
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
authors.forename: D M
authors.initials: DM
authors.lastname: Araujo
authors.forename: B
authors.initials: B
authors.lastname: Collier
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002415:Cats; D005260:Female; D005728:Ganglia, Sympathetic / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects*; D011957:Receptors, Opioid / Q000187:drug effects*
chemical_list: D002132:Calcium Radioisotopes; D011942:Receptors, Adrenergic, alpha; D011957:Receptors, Opioid; D000109:Acetylcholine; D002118:Calcium; D009638:Norepinephrine