Presynaptic inhibition of synaptic potentials evoked in cat spinal motoneurones by impulses in single group Ia axons.
1. Single-fibre group Ia excitatory post-synaptic potentials (e.p.s.p.s) were evoked in triceps surae motoneurones. These e.p.s.p.s were reduced by conditioning stimulation of group I axons in posterior biceps-semitendinosus nerves. 2. The investigation concentrated on e.p.s.p.s of somatic origin, because the amplitude of these e.p.s.p.s is not reduced by post-synaptic conductance increases. Any reduction in these e.p.s.p.s could therefore be attributed to presynaptic inhibition. 3. The reduction in somatic e.p.s.p. amplitude was greatest when the conditioning stimulus preceded the e.p.s.p. by 30 ms, and was negligible when the conditioning interval was extended to 200-300 ms. 4. The percentage reduction of somatic e.p.s.p.s was independent of their unconditioned peak amplitude. 5. E.p.s.p.s of somatic origin were reduced by the same amount, on average, as e.p.s.p.s of dendritic origin. 6. E.p.s.p.s evoked in the same motoneurone by impulses in different Ia axons were reduced by different amounts and e.p.s.p.s evoked in different motoneurones by impulses in the same Ia axon were also reduced by different amounts. 7. Analysis of fluctuations in e.p.s.p.s before and after conditioning indicated that after conditioning, larger discrete amplitudes became less probable, while smaller discrete amplitudes became more probable. The average increment between discrete amplitudes did not alter; nor were the discrete amplitudes reduced. 8. The probabilities of transmitter release at synaptic boutons were calculated before and during presynaptic inhibition. The maximum decrease in release probability was 0.64, suggesting a reduction in calcium influx of 10-15%.
Clements
J D
JD
Experimental Neurology Unit, John Curtin School of Medical Research, Australian National University, Canberra, A.C.T.
Forsythe
I D
ID
Redman
S J
SJ
D000818:Animals; D001369:Axons / Q000502:physiology; D002415:Cats; D005071:Evoked Potentials; D008564:Membrane Potentials; D009046:Motor Neurons / Q000502:physiology*; D009433:Neural Inhibition; D009475:Neurons, Afferent / Q000502:physiology; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission; D013997:Time Factors