title: Nucleotide sequence of the Australian bluetongue virus serotype 1 RNA segment 10.
abstract: The complete nucleotide sequence of the RNA segment 10 of Australian BTV serotype 1 has been deduced from a combination of sequencing cDNA inserts cloned into pBR322 and synthetic deoxynucleotide priming on purified double-stranded RNA molecules. The gene segment was 822 nucleotides in length and capable of coding for two proteins on either 229 or 216 amino acids, having net charges of either +4.5 or +5.5 respectively at neutral pH. Comparison with the RNA segment 10 from BTV serotype 10 revealed a high degree of amino acid sequence conservation as well as regions of nucleic acid sequence conservation.
authors.affiliation: CSIRO, Australian Animal Health Laboratory, Victoria.
authors.forename: A R
authors.initials: AR
authors.lastname: Gould
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001820:Bluetongue virus / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012087:Reoviridae / Q000235:genetics*
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D004247:DNA