Yeast/herpes simplex virus thymidine kinase gene fusions yield fusion proteins with thymidine kinase activity.
Expression in Saccharomyces cerevisiae of the Herpes Simplex Virus type-1 (HSV-1) thymidine kinase gene was accomplished by the construction of a gene fusion between the TK and a yeast gene. The fusion of yeast DNA sequences (which include a promoter and DNA that codes for the amino terminus end of the yeast gene product) with the TK gene resulted in a protein fusion with thymidine kinase activity.
McNeil
J B
JB
Department of Biology, York University, Downsview, Ontario, Canada.
Little
J G
JG
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism
D013937:Thymidine Kinase