title: Generation of human myogenic cell lines by the transformation of primary culture with origin-defective SV40 DNA.
abstract: The gene transfection technique was applied to establish clonal human skeletal muscle cell lines. DNA of a replication origin-defective mutant of SV40 was transfected into a primary culture of human skeletal muscle by the DNA-calcium phosphate co-precipitation method, and myoblast-derived cells were selected from among the transformed cells and cloned. The myogenic clonal cells exhibited an enhanced growth rate and an unlimited life span, which indicated that a stable supply of a large quantity of cultured human myogenic cells without contaminating fibroblasts was possible. In addition, despite the transformation, the transformed clones retained a certain differentiation ability, that is, they could form multinucleated cells or express a muscle-specific isomer of creatine kinase. These characteristics of transformed myogenic cells should be of great value in studies on the molecular pathologies of various myopathies.
authors.affiliation: Department of Pediatrics, Jichi Medical School, Tochigi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakamigawa
authors.forename: M Y
authors.initials: MY
authors.lastname: Momoi
authors.forename: T
authors.initials: T
authors.lastname: Momoi
authors.forename: M
authors.initials: M
authors.lastname: Yanagisawa
mesh_terms: D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D004279:DNA, Viral; D006801:Humans; D007223:Infant; D008297:Male; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D003402:Creatine Kinase