title: A monoclonal antibody to SV40 large T-antigen labels a nuclear antigen in JC virus-transformed cells and in progressive multifocal leukoencephalopathy (PML) brain infected with JC virus.
abstract: Thirty monoclonal antibodies to SV40 large T-antigen were tested for reactivity on the JC virus-transformed hamster glial cell line known as HJC-15. Two of them (PAb 416 and PAb 108) detected a nuclear antigen in both SV40-transformed CCL 75.1 cells and in HJC-15 cells, but not in control cells lacking T-antigen. These same antibodies also labeled a nuclear antigen in hamster tumor tissue derived from HJC-15 cells. In addition, the monoclonal antibody PAb 416 detected a nuclear antigen in progressive multifocal leukoencephalopathy (PML) tissue infected with JC virus, but not in normal brain tissue or tissue from other neurological diseases. Staining by PAb 416 was reduced by prior incubation with hamster anti-JCV tumor serum, suggesting that the polyclonal antiserum to JCV T-antigen may compete for an epitope at or near the PAb 416 binding site.
authors.affiliation: Laboratory of Experimental Neuropathology, NINCDS, Bethesda, MD 20892.
authors.forename: G L
authors.initials: GL
authors.lastname: Stoner
authors.forename: C F
authors.initials: CF
authors.lastname: Ryschkewitsch
authors.forename: D L
authors.initials: DL
authors.lastname: Walker
authors.forename: D
authors.initials: D
authors.lastname: Soffer
authors.forename: H D
authors.initials: HD
authors.lastname: Webster
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001927:Brain Diseases / Q000276:immunology; D002467:Cell Nucleus / Q000276:immunology; D002472:Cell Transformation, Viral; D006224:Cricetinae; D006801:Humans; D007124:Immunoenzyme Techniques; D007577:JC Virus / Q000276:immunology*; D007968:Leukoencephalopathy, Progressive Multifocal / Q000276:immunology*; D011120:Polyomavirus / Q000276:immunology*; D014412:Tumor Virus Infections / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming; D000956:Antigens, Viral