Isolation and characterization of Pseudomonas putida R-prime plasmids.
A number of enhanced chromosome mobilizing (ECM) plasmids derived from the wide host range plasmid R68 have been used to construct R-prime plasmids carrying a maximum of two map minutes of the Pseudomonas putida PPN chromosome, using Pseudomonas aeruginosa PAO as the recipient. For one ECM plasmid, pMO61, the ability to form R-primes did not correlate with the ability to mobilize chromosomes in intrastrain crosses, suggesting that different mechanisms are involved. Physical analysis of one R-prime showed that 3.5 kb of chromosomal DNA had been inserted between the tandem IS21 sequences carried by the parent ECM plasmid.
Bray
R
R
Department of Genetics, Monash University, Clayton, Victoria, Australia.
Strom
D
D
Barton
J
J
Dean
H F
HF
Morgan
A F
AF
D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011549:Pseudomonas / Q000235:genetics*; D011815:R Factors
D004251:DNA Transposable Elements; D004269:DNA, Bacterial