Genetic rescue of muscle defects associated with a mutant Drosophila melanogaster tropomyosin allele.
We describe a genetic transformation system which should prove useful for investigating tropomyosin assembly and function. Muscle abnormalities associated with a defective tropomyosin allele were corrected by integrating the wild-type gene into germ line chromosomes. The transformation protocol permits application of directed mutagenesis techniques in investigations of contractile regulatory mechanisms.
Fyrberg
E A
EA
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
Karlik
C C
CC
D000483:Alleles; D000818:Animals; D004262:DNA Restriction Enzymes; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics*; D005796:Genes; D009132:Muscles / Q000033:anatomy & histology; D009154:Mutation; D014335:Tropomyosin / Q000235:genetics*
D014335:Tropomyosin; D004262:DNA Restriction Enzymes