Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans.
A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacZ of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E. coli photolyases, notwithstanding the difference in chromophore structure.
Yasui
A
A
Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan.
Takao
M
M
Oikawa
A
A
Kiener
A
A
Walsh
C T
CT
Eker
A P
AP
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics*; D005796:Genes; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid
D004262:DNA Restriction Enzymes; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase