title: Inhibition of the streptavidin-biotin interaction by milk.
abstract: Nonfat dried milk is routinely used as a blocking agent and diluent in immunoassays. However, the data presented in this paper demonstrate that milk contains an inhibitor of the biotin-streptavidin interaction. It is recommended that milk be dialyzed or used at lower concentrations when it is employed as a diluent of streptavidin.
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: W L
authors.initials: WL
authors.lastname: Hoffman
authors.forename: A A
authors.initials: AA
authors.lastname: Jump
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis*; D001710:Biotin / Q000032:analysis*; D015153:Blotting, Western; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003956:Dialysis; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D008892:Milk / Q000032:analysis*; D019809:Streptavidin
chemical_list: D001426:Bacterial Proteins; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D001710:Biotin; D019809:Streptavidin; D006735:Horseradish Peroxidase