Modulation of protein kinase C activity by NaF in bone marrow derived macrophages.
Stimulation of murine bone marrow derived macrophages with NaF, prelabeled with [1-14C]oleate and [3H]inositol, increased the production of inositol phosphates and the release of 1,2-[14C]diacylglycerol (DAG). Moreover, NaF also induced activation of protein kinase C. These results indicate that bone marrow derived macrophages exhibit a phosphatidyl-4,5-bisphosphate phospholipase C activity, sensitive to NaF, which might be modulated by G-proteins. Activation of protein kinase C could have been mediated by NaF-induced release of DAG.
Hauschildt
S
S
Institut für Immunbiologie, Universität Freiburg, FRG.
Hirt
W
W
Bessler
W
W
D000818:Animals; D001854:Bone Marrow Cells; D004075:Diglycerides / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000096:biosynthesis; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology*
C011439:1,2-diacylglycerol; D004075:Diglycerides; D007295:Inositol Phosphates; D009829:Oleic Acids; D019301:Oleic Acid; D007294:Inositol; D012969:Sodium Fluoride; D011493:Protein Kinase C; D010727:Phosphoric Diester Hydrolases; D043265:Phosphatidylinositol Diacylglycerol-Lyase