The role of vitamin D3 in the proliferation of a human colon cancer cell line in vitro.
LoVo, a cultured colon cancer cell line, is shown to possess a receptor for 1,25-dihydroxy vitamin D3 (1 alpha,25(OH)2D3) with a low capacity (28 fmol/mg protein) and high affinity (Kd: 1.9 x 10(-21)0M). When these cells were grown in monolayer culture in a chemically defined serum-free medium, a significant inhibition of proliferation was seen in the presence of 10 nM to 1 microM of 1 alpha,25(OH)2D3 (p less than 0.005. Furthermore, 1 alpha,25(OH)2D3 delayed early attachment of cells. After 8 days of treatment, aggregated cuboidal cells showed a marked change to an apparently spindle like morphology. The 1 alpha,25(OH)2D3 growth-inhibitory effect was modulated by verapamil (1 microM), a calcium channel blocker, hydrocortisone (1 microM), and moxestrol (1 mM), an estrogen analogue, and 2% charcoal-treated fetal bovine serum. This study represents the first demonstration of 1 alpha,25(OH)2D3 modulation of growth of human colon cells.
Lointier
P
P
M. D. Anderson Hospital and Tumor Institute, Section of Gastrointestinal Oncology and Digestive Diseases, Houston, Texas 77030.
Wargovich
M J
MJ
Saez
S
S
Levin
B
B
Wildrick
D M
DM
Boman
B M
BM
D002117:Calcitriol / Q000494:pharmacology*; D002277:Carcinoma / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D004997:Ethinyl Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D006131:Growth Inhibitors; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000494:pharmacology
D006131:Growth Inhibitors; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D004997:Ethinyl Estradiol; C011310:moxestrol; D014700:Verapamil; D002117:Calcitriol; D006854:Hydrocortisone