Interleukin 3 stimulates phosphatidylcholine turnover in a mast/megakaryocyte cell line.
The hemopoietic growth factor, interleukin 3, has been shown to activate protein kinase C without causing hydrolysis of inositol phospholipids. The potential involvement of phosphatidylcholine hydrolysis as an alternative source of diacylglycerol was investigated in an interleukin 3-dependent murine mast/megakaryocyte cell line, R6-XE.4. Treatment of these cells with interleukin 3 rapidly stimulated both the release of water-soluble choline metabolites and the resynthesis of phosphatidylcholine. Therefore, a phosphatidylcholine cycle may operate as part of the signal transduction pathway in cells responding to interleukin 3.
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Biomedical Research Centre, University of British Columbia, Vancouver, Canada.
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Pelech
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D000818:Animals; D002460:Cell Line; D002794:Choline / Q000378:metabolism; D007377:Interleukin-3 / Q000494:pharmacology*; D007700:Kinetics; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D008533:Megakaryocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010713:Phosphatidylcholines / Q000378:metabolism*; D014316:Tritium
D007377:Interleukin-3; D010713:Phosphatidylcholines; D014316:Tritium; D002794:Choline