Synovial fluid hydroxyapatite crystals: detection thresholds of two methods.
A method of synovial fluid preparation giving optimal hydroxyapatite detection as well as definitions of the threshold masses of hydroxyapatite in viscous synovial fluid detectable by x ray diffraction and scanning electron microscopy with energy dispersive analysis is reported. Use of an equal volume of 100% hydrazine with the synovial fluid optimised detection of hydroxyapatite. By x ray diffraction the threshold mass of hydroxyapatite was 500 micrograms and by scanning electron microscopy with associated energy dispersive analysis 5 micrograms.
Cunningham
T
T
Division of Rheumatology, Hôpital Cantonal Universitaire, Geneva, Switzerland.
Uebelhart
D
D
Very
J M
JM
Fallet
G H
GH
Vischer
T L
TL
D002498:Centrifugation; D006801:Humans; D006882:Hydroxyapatites / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D013582:Synovial Fluid / Q000032:analysis*; D014961:X-Ray Diffraction
D006882:Hydroxyapatites