[A fluorimetric method for the determination of brain aminopeptidases].
A fluorometric method adapted to the determination of brain aminopeptidase activities has been developed in our laboratory. This method is based in the fluorescence produced by 2-naphthylamine when it is released from aminoacyl-2-naphthylamides under the hydrolytic action of these enzymes. Values of enzymatic activities and kinetic constants are similar to those previously described in the literature.
Alba
F
F
Iríbar
C
C
Ramírez
M
M
Arenas
C
C
D000626:Aminopeptidases / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D005470:Fluorometry / Q000379:methods; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000626:Aminopeptidases
Un método fluorimétrico para la determinación de aminopeptidasas cerebrales.