The role of catalytic iron in asbestos induced lipid peroxidation and DNA-strand breakage in C3H10T1/2 cells.
The involvement of catalytic iron in the vitro activities of crocidolite asbestos has been investigated. Exposure of C3H10T1/2 cells to either the UICC crocidolite standard reference sample or a non fibrous (milled) derivative resulted in an increase of thiobarbituric acid reactive substances. This catalytic activity was inhibited by pretreatment with the iron chelator desferrioxamine. The effect of this activity on cellular DNA was measured in an assay based on the production of DNA-strand breaks. Increased levels of DNA-strand breaks were detected in cultures treated with both the milled and UICC crocidolite. Inclusion of desferrioxamine with the asbestos inhibited DNA-strand breakage. It is concluded the catalytic iron present on the dust is capable of damaging both lipid and DNA and that this could be an important mechanism in asbestos pathogenicity.
Turver
C J
CJ
MRC Toxicology Unit, Carshalton, Surrey, UK.
Brown
R C
RC
D001194:Asbestos / Q000494:pharmacology*; D017638:Asbestos, Crocidolite; D002460:Cell Line; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D003676:Deferoxamine / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism*; D013858:Thiobarbiturates / Q000096:biosynthesis
D008054:Lipid Peroxides; D013858:Thiobarbiturates; D017638:Asbestos, Crocidolite; D001194:Asbestos; D004247:DNA; D003676:Deferoxamine; C029684:thiobarbituric acid