Restriction mapping and hybridization studies of a beta-lactamase-encoding fragment from Streptococcus (Enterococcus) faecalis.
A restriction map of a 5.1-kilobase EcoRI fragment encoding an enterococcal beta-lactamase was prepared and compared with the restriction map of a cloned staphylococcal beta-lactamase gene and with the published maps of several other staphylococcal beta-lactamase genes. Comparison and hybridization studies showed that there were identical restriction sites in the region of the bla structural gene but not in the region surrounding this gene.
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Program in Infectious Diseases and Clinical Microbiology, University of Texas Medical School, Houston 77030.
Hull
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Murray
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D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D013293:Enterococcus faecalis / Q000235:genetics*; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D001618:beta-Lactamases