title: Swiveling and decatenation of replicating simian virus 40 genomes in vivo.
abstract: We have found that type II topoisomerase inhibitors have two effects on replicating simian virus 40 genomes in vivo: production of catenated dimers and slowed replication of the last 5% of the genome. This suggests that type II topoisomerase simultaneously decatenates and facilitates replication fork movement at this stage of DNA replication. On the basis of this observation, a detailed model is proposed for the roles of topoisomerases I and II in simian virus 40 DNA replication.
authors.affiliation: Department of Radiology, Ohio State University, Columbus 43210.
authors.forename: R M
authors.initials: RM
authors.lastname: Snapka
authors.forename: M A
authors.initials: MA
authors.lastname: Powelson
authors.forename: J M
authors.initials: JM
authors.lastname: Strayer
mesh_terms: D000818:Animals; D002166:Camptothecin / Q000494:pharmacology; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D005047:Etoposide / Q000494:pharmacology; D005814:Genes, Viral / Q000187:drug effects; D007364:Intercalating Agents / Q000494:pharmacology; D013539:Simian virus 40 / Q000235:genetics*; D013713:Teniposide / Q000494:pharmacology; D059005:Topoisomerase II Inhibitors; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D007364:Intercalating Agents; D059005:Topoisomerase II Inhibitors; D005047:Etoposide; D013713:Teniposide; D002166:Camptothecin