Isolation from rat brain tissue of an inhibiting activity for dihydropyridine binding sites and voltage-dependent CA2+ uptake.
Nitrendipine binding-inhibitory activity was extracted and partially purified from rat brain. This preparation also decreased veratridine-stimulated Ca2+ uptake in cultured cerebellar granule cells. It failed to inhibit 3H-nitrendipine binding by sequestering Ca2+ that is required for the high affinity binding of nitrendipine. An allosteric modulation of nitrendipine recognition sites by this preparation is suggested.
Sanna
E
E
Hypertension-Endocrine Branch, NHLBI National Institutes of Health, Bethesda, MD 20892.
Hanbauer
I
I
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels; D004095:Dihydropyridines / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D066298:In Vitro Techniques; D009568:Nitrendipine / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000378:metabolism*
D015220:Calcium Channels; D004095:Dihydropyridines; D011978:Receptors, Nicotinic; D009568:Nitrendipine; D002118:Calcium