Doxorubicin-induced altered glycolytic patterns in the leukemic cell studied by proton spin echo NMR.
1H spin echo NMR of the leukemic cell line J-lll is reported. Phosphorylcholine, phosphorylcreatine, lactate and mobile triglyceride are identified as constituents of the cytosolic pool in the intact and viable cells. The concentration of cells in the NMR tube is high (approx. 10(9) in 0.4 ml) and as a result the available oxygen is restricted, making the NMR experiment a plausible in vitro tumour model in which kinetics in the living cell can be probed in a non-invasive manner. Treatment with pharmacological concentrations of the drug doxorubicin produced immediate enhanced anaerobic glycolysis and eventual cell death.
Reglinski
J
J
Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, Scotland, UK.
Smith
W E
WE
Suckling
C J
CJ
Al-Kabban
M
M
Stewart
M J
MJ
Watson
I D
ID
D004317:Doxorubicin / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects*; D007773:Lactates / Q000378:metabolism; D007938:Leukemia / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011522:Protons; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D007773:Lactates; D011522:Protons; D004317:Doxorubicin