Degradation of aflatoxin by Aspergillus flavus.
Aflatoxin degradative activity was demonstrated in 6- to 12-d-old intact mycelium and cell-free extracts of Aspergillus flavus. The addition of cycloheximide, SKF 525-A or metyrapone to cultures of A. flavus prevented subsequent degradation of the aflatoxins, while in cell-free extracts degradation was inhibited by SKF 525-A, metyrapone and cytochrome c but not by KCN. In cell-free extracts, aflatoxin degradation was enhanced by NADPH and NaIO4. The results suggest the involvement of cytochrome P-450 monooxygenases in the aflatoxin degradative activity of A. flavus.
Hamid
A B
AB
Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, UK.
Smith
J E
JE
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism*; D001231:Aspergillus flavus / Q000187:drug effects / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D003574:Cytochrome c Group / Q000494:pharmacology; D008797:Metyrapone / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D011335:Proadifen / Q000494:pharmacology
D000348:Aflatoxins; D003574:Cytochrome c Group; D010504:Periodic Acid; C027955:aflatoxin G1; D009249:NADP; D003513:Cycloheximide; D016604:Aflatoxin B1; D011335:Proadifen; C009288:metaperiodate; D011190:Potassium Cyanide; D008797:Metyrapone