cDNA cloning of human chorionic somatomammotropin-1 mRNA whose transcription was initiated at the 5' region of the TATA box.
cDNA of human chorionic somatomammotropin (hCS) mRNA, whose transcription had been initiated 26 nucleotides upstream from the ordinary TATAAA sequence, was cloned from term placenta. This variant cDNA clone was 56 nucleotides longer than the usual one at the 5'-noncoding region, consistent with the previous report that placental poly(A+) RNA contains longer transcripts probably directed by a CATAAA sequence located 5' to the TATAAA sequence (Selby, M.J., Barta, A., Baxter, J.D., Bell, G.I., and Eberhardt, N.L. (1984) J. Biol. Chem. 259, 13131-13138). The nucleotide sequence analysis revealed that the variant cDNA clones were derived form the hCS-1 gene but not from the hCS-2 gene. The variant clones accounted for approximately 9.7% of the total hCS-1 cDNA clones. In the 5'-flanking region around the TATAAA sequence, the hCS-1 gene showed a less stable dyad symmetry structure as compared with that of the hCS-2 gene.
Tanaka
M
M
Department of Biochemistry, Mie University School of Medicine, Japan.
Masuda
N
N
Watahiki
M
M
Yamakawa
M
M
Shimizu
K
K
Nagai
J
J
Nakashima
K
K
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010928:Placental Lactogen / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger; D014158:Transcription, Genetic
D012333:RNA, Messenger; D004247:DNA; D010928:Placental Lactogen; D004262:DNA Restriction Enzymes