A method for determination of glucose 1,6-bisphosphatase.
A sensitive and specific method to measure glucose 1,6-bisphosphatase activity, which allows the identification of the reaction products is described. [U-14 C]Glucose 1,6-P2, synthesized by the glucose 1-P kinase activity of phosphofructokinase, is used as substrate. The reaction products are separated and identified by chromatography on ion-exchange paper.
Bassols
A M
AM
Department de Ciències Fisiològiques Humanes i de la Nutrició, Facultat de Medicina, Universitat de Barcelona, Spain.
Carreras
J
J
Cussó
R
R
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002852:Chromatography, Ion Exchange; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000302:isolation & purification / Q000378:metabolism; D009132:Muscles / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis* / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D051381:Rats
D005958:Glucosephosphates; D019298:Glucose-6-Phosphate; D000255:Adenosine Triphosphate; C031590:glucose-1-phosphate; C016473:glucose-1,6-bisphosphate; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C046331:phosphoglucokinase; C014818:glucose 1,6-diphosphate phosphatase; D010744:Phosphoric Monoester Hydrolases