The terminus of SV40 DNA replication and transcription contains a sharp sequence-directed curve.
We have examined nucleosome positioning on two DNA segments containing sharp sequence-directed curvatures. A 223 bp DNA from Crithidia fasciculata was cloned into two sites in pBR325 separated by 28%. These sites were found to selectively reconstitute nucleosomes 5- to 7-fold more effectively than the adjoining straight DNA. The terminus of replication and termini of transcription of SV40 DNA are contained within a region of approximately 200 bp centrally located in a 1216 bp fragment. Visualization of this fragment by electron microscopy revealed a sharp curve of approximately 200 degrees in the terminal region. Reconstitution of histone protein with this fragment revealed a 2- to 5-fold higher probability of assembling nucleosomes in the terminal region over the adjacent DNA.
Hsieh
C H
CH
Lineberger Cancer Research Center, University of North Carolina Medical School, Chapel Hill 27514.
Griffith
J D
JD
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003421:Crithidia / Q000235:genetics*; D004261:DNA Replication; D004279:DNA, Viral; D004586:Electrophoresis; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000502:physiology*; D011120:Polyomavirus / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; D009707:Nucleosomes