Lupus anticoagulant activities of murine monoclonal antibodies to liposomal phosphatidylinositol phosphate.
Four murine monoclonal antibodies having high levels of activity against phosphatidyl-inositol phosphate (PIP) were tested for lupus anticoagulant activity. The antibodies showed different degrees of potency in a modified partial thromboplastin time test (APTT) that used dilutions of either bovine brain extract (Thrombofax) or liposomes consisting of phosphatidylcholine/phosphatidylserine (PC/PS) as the phospholipid source. The same relative order of anticoagulant potency that was observed in the APTT that used the PC/PS liposomes was maintained when the anti-PIP antibodies were tested for cross-reactivity either by induction of complement-dependent immune damage to liposomes containing PS, or in enzyme-linked immunosorbent assays that used PS, cardiolipin (CL), or phosphatidylinositol (PI) as antigens. The data indicate that monoclonal antibodies to PIP can express anticoagulant activity in a modified APTT that correlates with their different degrees of cross-reactivity against the negatively-charged phospholipids PS, CL, and PI.
Alving
B M
BM
Department of Hematology, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Banerji
B
B
Fogler
W E
WE
Alving
C R
CR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D008081:Liposomes / Q000276:immunology*; D016682:Lupus Coagulation Inhibitor; D051379:Mice; D008807:Mice, Inbred BALB C; D010314:Partial Thromboplastin Time; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001779:Blood Coagulation Factors; D008081:Liposomes; D016682:Lupus Coagulation Inhibitor; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; C037178:phosphatidylinositol 4-phosphate