Increased cAMP production after short-term capacitively coupled stimulation in bovine growth plate chondrocytes.
Growth plate chondrocytes from newborn calf costochondral junctions grown in monolayer were subjected to a capacitive AC signal of 500 V peak to peak (P-P) at 60 kHz for 48 h and were analyzed for [3H]thymidine uptake. The stimulated chondrocytes showed a 130% greater uptake over unstimulated controls. Other newborn calf growth plate chondrocytes were stimulated at 500 V P-P at 60 kHz for 2.5, 5.0, 10.0, and 20.0 min and were analyzed for cAMP. Chondrocytes stimulated for 2.5 and 5.0 min showed a 142.8% (p less than 0.05) and 394.5% (p less than 0.01) increase over controls, respectively. The chondrocytes stimulated for 10.0 and 20.0 min showed no significant difference from the controls. It is concluded that short-term exposure of growth plate chondrocytes to an appropriate capacitively coupled field stimulates cAMP production, but longer-term application of the electrical field is ineffective.
Brighton
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CT
Department of Orthopaedic Surgery, University of Pennsylvania School of Medicine, Philadelphia 19104.
Townsend
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PF
D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D000242:Cyclic AMP / Q000096:biosynthesis*; D004558:Electric Stimulation; D006132:Growth Plate / Q000378:metabolism*; D066298:In Vitro Techniques; D012272:Ribs; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000242:Cyclic AMP; D013936:Thymidine