Activation of synthesis of hexacosenoic acid by sulfhydryl reagents in swine cerebral microsomes.
The elongation of icosenoyl-CoA (20:1-CoA) in swine cerebral microsomes resulted in the synthesis of docosenoic acid (22:1) and tetracosenoic acid (24:1), but the synthesis of hexacosenoic acid (26:1) was negligible. In contrast, in the presence of sulfhydryl reagents (0.6 mM N-ethylmaleimide [NEM] or 0.3 mM p-chloromercuriphenylsulfonic acid [PCMPS]) the synthesis of 26:1 was remarkably enhanced. We suggest that the synthesis of 26:1 from 20:1-CoA was more enhanced by NEM or PCMPS as a result of activation of the condensation step in the elongation of 24:1 (intermediate) to 26:1.
Saitoh
T
T
Department of Biochemistry, Medical College of Oita, Japan.
Yoshida
S
S
Takeshita
M
M
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis; D005229:Fatty Acids, Monounsaturated / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D013552:Swine
D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D013439:Sulfhydryl Reagents; C040938:1-hexacosenoic acid; D002731:4-Chloromercuribenzenesulfonate; D005033:Ethylmaleimide