DNA damage/repair studies using a combination of monoclonal thymidine antibody and unscheduled DNA synthesis simultaneously.
The ability of a new monoclonal antibody against thymidine (MoAb 20B7) to detect chemotherapy induced single stranded DNA damage is described. HL-60 cells were used for these experiments. Damage to DNA was caused by incubation of cells with alkylating agents. Portions of DNA which is damaged are cleaved by cellular endonucleases, thus exposing thymidine on the opposite DNA strand. Processing of these samples by MoAb 20B7 showed that such damaged segments could be detected consistently. Furthermore, this method was combined with autoradiographic detection of unscheduled DNA synthesis thereby allowing for assessment of DNA repair simultaneously from the same cell.
Soni
N
N
Roswell Park Memorial Institute, Department of Hematologic Oncology, Buffalo, NY.
Mehdi
I
I
Motyka
L
L
Mayers
G
G
Raza
A
A
D000911:Antibodies, Monoclonal; D002460:Cell Line; D004249:DNA Damage; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis* / Q000187:drug effects; D003630:Daunorubicin / Q000494:pharmacology; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008466:Mechlorethamine / Q000494:pharmacology; D013936:Thymidine / Q000032:analysis*
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D008466:Mechlorethamine; D013936:Thymidine; D003630:Daunorubicin