title: An effective approach to the identification of subclones of a DNA of known nucleotide sequence.
abstract: The problem of subclone identification for DNA fragments of a known nucleotide sequence has been considered. We suggest a strategy for rapid identification of a large number of subclones based on: (i) partial sequencing of the subclone DNA (single nucleotide track); (ii) representation of the result in the form of a numeric code showing the distribution of the chosen nucleotide along the sequence; and (iii) identification of the subclone sequence using this code in a catalogue compiled and printed for a whole DNA sequence. The same approach is applicable when the subclones are expected to have homology with known sequences.
authors.affiliation: Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.
authors.lastname: Golovanov EuI
authors.forename: S A
authors.initials: SA
authors.lastname: Nedospasov
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004279:DNA, Viral; D001330:Electronic Data Processing; D013539:Simian virus 40 / Q000235:genetics
chemical_list: D004279:DNA, Viral; D004247:DNA