A reappraisal of the subunit molecular mass of chicken liver cytosol 5'-nucleotidase.
The subunit molecular mass of chicken liver cytosol 5'-nucleotidase was earlier reported to be 51 kDa upon sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (Naito, Y. and Tsushima, K. (1976) Biochim. Biophys. Acta 438, 159-168). By immunoblot analyses after SDS-gel electrophoresis, however, a fraction from the liver homogenized in the presence of leupeptin showed multiple bands with molecular masses around 57 kDa, and SDS-extracted proteins directly from the liver exhibited a single 70 kDa component. These results indicate that the 51 kDa peptide observed in the cytosol 5'-nucleotidase preparation might, in fact, be a proteolytic artifact.
Oka
J
J
Division of Adult Nutrition, National Institute of Nutrition, Tokyo, Japan.
Ozasa
H
H
Itoh
R
R
D015720:5'-Nucleotidase; D000818:Animals; D002645:Chickens; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D009708:Nucleotidases / Q000302:isolation & purification*
D046911:Macromolecular Substances; D009708:Nucleotidases; D015720:5'-Nucleotidase