title: [3H]diazepam specific binding to rat cortex in vitro is enhanced by oleic, arachidonic and docosahexenoic acid isolated from pig brain.
abstract: Substances were found in purified fractions from pig brain that enhanced the specific binding of [3H]diazepam to membranes from rat brain in vitro. These substances were identified as oleic acid, arachidonic acid and docosahexenoic acid. Oleic acid (10(-5) - 10(-4)M) increased the affinity for agonists binding to the benzodiazepine receptor, whereas the binding of antagonists to this receptor was only slightly enhanced. The number of [3H]muscimol binding sites was increased, whereas binding of [3H]SR 95331, a GABA receptor antagonist, was unchanged. The effect of oleic acid was additional to the GABA-induced enhancement of [3H]diazepam binding.
authors.affiliation: Psychopharmacological Research Laboratory, Section Hans Hospital, Roskilde, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Nielsen
authors.forename: M R
authors.initials: MR
authors.lastname: Witt
authors.forename: H
authors.initials: H
authors.lastname: Thøgersen
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000302:isolation & purification / Q000494:pharmacology; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D003975:Diazepam / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000302:isolation & purification / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D019301:Oleic Acid; D009829:Oleic Acids / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D013552:Swine
chemical_list: D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D009829:Oleic Acids; D011963:Receptors, GABA-A; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D019301:Oleic Acid; D003975:Diazepam