Contact sensitivity induced in mice by methylene bisphenyl diisocyanate.
An experimental model of contact sensitivity has been developed in C57BL/6 mice using methylene bisphenyl diisocyanate (MDI), a raw material of polyurethane resins. Sensitization through a single epidermal application of 1% MDI solution in ethyl acetate to the backs of the mice resulted in marked ear swelling. The time course of the swelling was characteristic of delayed-type hypersensitivity and the increment of the ear thickness was compatible with that induced by toluene diisocyanate (TDI). Passive transfer of the MDI-induced contact sensitivity was successfully achieved using lymphocytes from the lymph nodes of MDI-sensitized syngeneic mice, and the effector cells were found to be T cells. Cross reaction between MDI and TDI has shown that MDI is not only a potent contact sensitizer but also can form a contact sensitizer group together with TDI.
Tanaka
K
K
Toxicology Laboratory, Toray Industries, Inc., Otsu, Japan.
Takeoka
A
A
Nishimura
F
F
Hanada
S
S
D000279:Administration, Cutaneous; D000818:Animals; D003429:Cross Reactions / Q000187:drug effects; D003485:Cyanates / Q000494:pharmacology / Q000633:toxicity*; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology; D004195:Disease Models, Animal; D006968:Hypersensitivity, Delayed / Q000139:chemically induced* / Q000276:immunology; D007116:Immunization, Passive; D017953:Isocyanates; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology; D001559:Benzhydryl Compounds
D003485:Cyanates; D017953:Isocyanates; D014051:Toluene 2,4-Diisocyanate; C005969:4,4'-diphenylmethane diisocyanate; D001559:Benzhydryl Compounds