Structure and expression of the gene coding for the human serpin hLS2.
We have analyzed genomic clones encoding human leuserpin 2 (hLS2). The gene covers about 14.5 kilobases and consists of 5 exons and 4 introns. The genes coding for hLS2, alpha 1-antitrypsin, alpha 1-antichymotrypsin, and rat angiotensinogen share an equivalent exon-intron structure and therefore constitute a distinct subgroup within the serpin gene family, which otherwise displays a highly variable exon-intron pattern. With the exception of a segment in the second exon, the sequence similarity of the genes coding for hLS2 and alpha 1-antitrypsin extends to all exons including one encoding the 5'-untranslated sequences. The implications of these findings with respect to the genesis of the amino-terminal heterogeneity in the serpin family are discussed.
Ragg
H
H
Hoechst AG, Frankfurt, Federal Republic of Germany.
Preibisch
G
G
D000595:Amino Acid Sequence; D000808:Angiotensinogen / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005786:Gene Expression Regulation; D015844:Heparin Cofactor II; D006801:Humans; D007163:Immunosorbent Techniques; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D000514:alpha 1-Antichymotrypsin / Q000235:genetics; D000515:alpha 1-Antitrypsin / Q000235:genetics
D003062:Codon; D011506:Proteins; C047932:SERPIND1 protein, human; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D000808:Angiotensinogen; D015844:Heparin Cofactor II; D004247:DNA; D004262:DNA Restriction Enzymes