title: Growth-rate-dependent expression and cloning of gnd alleles from natural isolates of Escherichia coli.
abstract: 6-Phosphogluconate dehydrogenase (6PGD), encoded by gnd, is highly polymorphic among isolates of Escherichia coli form natural populations. As a means of characterizing the growth-rate-dependent regulation of the level of 6PGD, five gnd alleles, including the E. coli B/r allele, were crossed into E. coli K-12 with bacteriophage P1. In each of the isogenic strains, the level of 6PGD was two- to threefold higher in cells grown on glucose than in cells grown on acetate. The level of enzyme activity in the acetate-grown cells varied about sixfold within the set of isogenic strains. The physiological importance of these differences in enzyme level is discussed. The gnd gene was cloned from five E. coli strains and Salmonella typhimurium LT-2 and mapped with twelve restriction endonucleases. gnd was located and oriented on the chromosomal DNAs. The restriction maps of the genes were aligned at conserved restriction sites, and the relative divergence of the genes was estimated from restriction site polymorphisms. The E. coli gnd genes differed from the S. typhimurium gene by about 11%. Most of the E. coli genes differed from one another by less than 5%, but one allele differed from the others by about 10%. Only the gnd gene from E. coli K-12 had an IS5 element located nearby.
authors.affiliation: Department of Biological Sciences, University of Maryland, Baltimore County 21228.
authors.forename: G J
authors.initials: GJ
authors.lastname: Barcak
authors.forename: R E
authors.initials: RE
authors.lastname: Wolf
mesh_terms: D000483:Alleles; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D010734:Phosphogluconate Dehydrogenase; D004262:DNA Restriction Enzymes