title: Structure of the knob protein (KP) gene of Plasmodium falciparum.
abstract: We have determined the nucleotide sequence of the gene encoding the knob protein (KP) of Plasmodium falciparum (FCR-3/Gambia). The gene is interrupted by an intron which contains 34 imperfect tandemly repeated ATTTT sequences. The first exon encodes 33 amino acids with a hydrophobic core typical of signal peptides. The second exon has an open translational reading frame for 597 amino acids. The deduced protein sequence indicates that KP has multiple structural domains; unlike the N-terminal histidine-rich domain which we described previously, the C-terminal half is rich in lysine residues. Consistent with the apparent association of KP with the cytoplasmic surface of the host erythrocyte membrane, the protein is highly charged and hydrophilic.
authors.affiliation: Public Health Research Institute, New York, NY 10016.
authors.forename: Y D
authors.initials: YD
authors.lastname: Sharma
authors.forename: A
authors.initials: A
authors.lastname: Kilejian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005091:Exons; D005796:Genes; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics*; D015800:Protozoan Proteins
chemical_list: D008565:Membrane Proteins; D010455:Peptides; D015800:Protozoan Proteins; C042968:knob protein, Plasmodium falciparum; D004247:DNA; D004262:DNA Restriction Enzymes