Recovery of DNA fragments specifically bound by monoclonal anti-DNA antibody.
A method to recover and identify DNA fragments that were specifically bound by a monoclonal anti-DNA antibody has been developed. A mixture of DNA fragments digested by restriction enzymes was first incubated with a murine monoclonal anti-DNA antibody and then reacted with anti-murine immunoglobulin-conjugated Sepharose 4B. The resulting complex was washed to remove unbound DNA by low-speed centrifugation. The bound DNA fragment was released from antibody by alkaline dimethyl sulfoxide solution or extracted by phenol treatment. The recovered DNA was analyzed by electrophoresis on a polyacrylamide gel.
Fukada
M
M
Department of Microbiology, Aichi Medical University, Japan.
Yoshikawa
K
K
Miyagawa
Y
Y
D000974:Antibodies, Antinuclear / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D009838:Oligodeoxyribonucleotides / Q000276:immunology / Q000302:isolation & purification*
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes