Aerobic expression of the nar operon of Escherichia coli in a fnr mutant.
Mutations allowing aerobic expression of the anaerobically controlled nar operon have been located in the autoregulated fnr gene. Cloning and sequencing of the mutant fnrd20 allele, and fnr mRNA quantitation by dot blot assay, revealed that the mutation was the result of an IS5 insertion into the control region of fnr that enhanced transcription of the fnr gene at least ten-fold. Examination of the regulatory region of the negatively autoregulated fnr gene indicated that it shared homologous sequences with the positively Fnr-controlled frd and nar operons. The increase in fnr transcription in the fnrd20 mutated allele could be partly the result of loss of autoregulation, since the IS5 separated the Fnr target site from the '-35' region of the promoter.
Bonnefoy
V
V
Laboratoire de Chimie Bactérienne, CNRS, Marseille, France.
Fons
M
M
Ratouchniak
J
J
Pascal
M C
MC
Chippaux
M
M
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009154:Mutation; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009876:Operon; D010100:Oxygen / Q000378:metabolism*; D010957:Plasmids; D014157:Transcription Factors / Q000378:metabolism; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D014157:Transcription Factors; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D001616:beta-Galactosidase; D010100:Oxygen