title: Monoclonal antibodies for dengue virus prM glycoprotein protect mice against lethal dengue infection.
abstract: Five murine monoclonal antibodies (Mabs) reactive against the prM glycoproteins of DEN-3 and -4 were used to passively protect mice in vivo against lethal challenge with homologous and heterologous dengue virus serotypes. Four of the 5 prM-reactive monoclonals cross-protected mice against heterologous challenge, whereas 1 protected against challenge with only the homologous serotype. Although in vitro binding to virions was readily demonstrated, only 2 of the prM Mabs had detectable neutralizing activity. The neutralizing activity could not be enhanced by anti-mouse immunoglobulin or complement. However, 4 of the 5 prM Mabs fixed complement. This is the first report of prM-specific Mabs that are protective in mice.
authors.affiliation: Walter Reed Army Institute of Research, Washington, DC.
authors.forename: B M
authors.initials: BM
authors.lastname: Kaufman
authors.forename: P L
authors.initials: PL
authors.lastname: Summers
authors.forename: D R
authors.initials: DR
authors.lastname: Dubois
authors.forename: W H
authors.initials: WH
authors.lastname: Cohen
authors.forename: M K
authors.initials: MK
authors.lastname: Gentry
authors.forename: R L
authors.initials: RL
authors.lastname: Timchak
authors.forename: D S
authors.initials: DS
authors.lastname: Burke
authors.forename: K H
authors.initials: KH
authors.lastname: Eckels
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D003715:Dengue / Q000276:immunology* / Q000517:prevention & control; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007116:Immunization, Passive; D009500:Neutralization Tests; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D015678:Viral Structural Proteins