title: Large scale isolation of nuclei from oocytes of Xenopus laevis.
abstract: We describe an improvement on the procedure of Scalenghe et al. (F. Scalenghe, M. Buscaglia, C. Steinheil, and M. Crippa (1978) Chromosoma 66, 299-308) for the large scale isolation of nuclei from Xenopus laevis oocytes. The nuclear extract obtained was tested for its ability to transcribe a cloned Xenopus 5 S RNA gene and for the presence of nuclear factors interacting with a X. laevis ribosomal protein gene promoter. Efficiency of accurate transcription and of factor binding is comparable with that of an extract prepared from manually isolated nuclei.
authors.affiliation: Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Ruberti
authors.forename: E
authors.initials: E
authors.lastname: Beccari
authors.forename: E
authors.initials: E
authors.lastname: Bianchi
authors.forename: F
authors.initials: F
authors.lastname: Carnevali
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus / Q000378:metabolism*; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000032:analysis; D005260:Female; D066298:In Vitro Techniques; D009865:Oocytes / Q000378:metabolism / Q000648:ultrastructure*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D014158:Transcription, Genetic; D014982:Xenopus laevis
chemical_list: D015342:DNA Probes; D004268:DNA-Binding Proteins; D012341:RNA, Ribosomal, 5S