title: Topography, purification and characterization of thyroidal 5'-nucleotidase.
abstract: 1. Subcellular studies of bovine thyroid indicate that 5'-nucleotidase is predominantly associated with plasma membranes, although a considerable part of this ectoenzyme is also found internalized. 2. The enzyme displaying the features of a glycoprotein has been purified 1400 times by detergent solubilization and two subsequent affinity chromatographic steps. 3. Thyroidal 5'-nucleotidase can be classified as an unspecific metallo-dependent 5'-ribonucleotide phosphohydrolase. The native enzyme exists as a dimer (MW 150 kDalton), composed of two similar or identical subunits.
authors.affiliation: RUCA-Laboratory for Human Biochemistry, University of Antwerp, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Peeters
authors.forename: M
authors.initials: M
authors.lastname: de Wolf
authors.forename: G
authors.initials: G
authors.lastname: Van Dessel
authors.forename: A
authors.initials: A
authors.lastname: Lagrou
authors.forename: H
authors.initials: H
authors.lastname: Hilderson
authors.forename: W
authors.initials: W
authors.lastname: Dierick
mesh_terms: D015720:5'-Nucleotidase; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009708:Nucleotidases / Q000302:isolation & purification* / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D013961:Thyroid Gland / Q000201:enzymology*
chemical_list: D009708:Nucleotidases; D015720:5'-Nucleotidase