Labeled avidin-biotin enzyme-linked immunosorbent assay for detecting antibody to infectious laryngotracheitis virus in chickens.
A labeled avidin-biotin enzyme-linked immunosorbent assay (LAB-ELISA) for detecting antibody to infectious laryngotracheitis (ILT) virus in chicken sera was developed and compared with ordinary ELISA. Purified ILT virus, biotin-labeled anti-chicken IgG rabbit IgG conjugate, and horseradish-peroxidase-labeled avidin were used in the LAB-ELISA. When sera from farm chickens were tested by serum neutralization (SN) and two kinds of ELISA, the correlation rate between SN and LAB-ELISA was 50/50 (100%), and that between SN and ordinary ELISA was 39/50 (78%). In LAB-ELISA, all of the sera that were antibody-negative by SN had low absorbance (A) values (below 0.05), and the A values were closely correlated with the SN indexes. In ordinary ELISA, however, the sera antibody-negative by SN had various A values ranging from 0.06 to 0.32. LAB-ELISA had much lower nonspecific reactions than ordinary ELISA against sera from ILT-negative chickens, even when chickens were 30 weeks old. ILT antibody production after ILT vaccination could be detected by LAB-ELISA. A values peaked 5 weeks postinoculation and were maintained for 17 weeks.
Ohkubo
Y
Y
Department of Animal Pathology, Nisshin Flour Milling Co. Ltd., Saitama, Japan.
Shibata
K
K
Mimura
T
T
Takashima
I
I
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001360:Avidin; D001710:Biotin; D002645:Chickens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006564:Herpesviridae / Q000276:immunology*; D001352:Herpesvirus 1, Gallid / Q000276:immunology*
D000914:Antibodies, Viral; D001360:Avidin; D001710:Biotin