Partial amino acid sequence of rat topoisomerase I: comparison with the predicted sequences for the human and yeast enzymes.
The partial amino acid sequence of rat topoisomerase I was determined by gas-phase microsequencing. Seven tryptic peptides closely matched the sequences deduced from human topoisomerase I cDNA (94.5% homology). Similarity to sequences deduced from baker's yeast and fission yeast genomic DNA were restricted to conserved domains which may represent important sites of interaction with DNA or with other proteins.
Durban
E
E
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
Bramucci
M
M
Cook
R
R
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000235:genetics; D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012568:Schizosaccharomyces / Q000201:enzymology; D013045:Species Specificity; D014357:Trypsin
D010446:Peptide Fragments; D004247:DNA; D014357:Trypsin; D004264:DNA Topoisomerases, Type I