title: Establishment of a new Epstein-Barr virus-immortalized cell line from chronic lymphocytic leukemia with trisomy of chromosome 12 that produces monoclonal IgM against a sheep RBC antigen.
abstract: Leukemia cells from a patient with chronic lymphocytic leukemia (CLL) were found to bind sheep RBC (SRBC) through their monoclonal surface IgM. A lymphoblastoid cell line was obtained by immortalization of leukemic cells with Epstein-Barr virus (EBV). Cultured leukemic cells were found to have a supernumerary chromosome 12, an abnormality typical of CLL of the B cell type. To our knowledge, this is the first EBV-immortalized cell line from B-CLL cells of known SRBC specificity and the third reported CLL cell line carrying trisomy of chromosome 12.
authors.affiliation: Department of Clinical Immunology, University of Rome, La Sapienza, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Crescenzi
authors.forename: M
authors.initials: M
authors.lastname: Napolitano
authors.forename: M
authors.initials: M
authors.lastname: Carbonari
authors.forename: A
authors.initials: A
authors.lastname: Antonelli
authors.forename: P
authors.initials: P
authors.lastname: Petrinelli
authors.forename: C
authors.initials: C
authors.lastname: Gaetano
authors.forename: M
authors.initials: M
authors.lastname: Fiorilli
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D002460:Cell Line; D002472:Cell Transformation, Viral; D002881:Chromosomes, Human, Pair 12; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology; D014314:Trisomy; D014407:Tumor Cells, Cultured / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000954:Antigens, Surface; D007075:Immunoglobulin M