Characterization of viral polypeptides from avian rotavirus.
The synthesis of avian rotavirus (AvRV-1) polypeptides in MA 104 cells was investigated. Extracts of cells labeled with either [35S]methionine or [3H]mannose were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Viral protein synthesis was detected first at 6 hours postinfection. Ten major viral polypeptides were detected at this time. The presence of five viral structural proteins (100K, 90K, 88K, 45K, and 37K) were demonstrated in AvRV-1-infected cells by immunoprecipitation analysis. One structural polypeptide (37K) was identified as a glycoprotein. Two viral polypeptides (30K and 28K) were identified as nonstructural glycoproteins. By tunicamycin inhibition of glycosylation, a 32K polypeptide was identified. This 32K polypeptide later was proven to be the precursor of 37K structural glycoprotein by immunoprecipitation analysis, beta-N-acetylglucosaminidase H (Endo H) treatment, and peptide mapping analysis. Avian rotavirus contained high-mannose oligosaccharide content in the glycoproteins similar to the glycoproteins of mammalian rotaviruses.
Kang
S Y
SY
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Nagaraja
K V
KV
Newman
J A
JA
D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis; D007118:Immunoassay; D007700:Kinetics; D010452:Peptide Biosynthesis; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis; D012401:Rotavirus / Q000378:metabolism*; D014422:Turkeys; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D015678:Viral Structural Proteins
D006023:Glycoproteins; D010455:Peptides; D014764:Viral Proteins; D015678:Viral Structural Proteins