In vitro transport of ornithine carbamoyltransferase precursor into rat liver mitochondria using a more homologous medium.
The precursor of ornithine carbamoyltransferase can be transported in vitro into rat liver mitochondria using the postmitochondrial supernatant from rat liver, a more homologous medium than the commonly used rabbit reticulocyte lysate. The transport of the precursor in the case of reticulocyte lysate requires a standard translation mixture. In the presence of the postmitochondrial supernatant the same is true. However, when the components of the translation mixture were added individually to the postmitochondrial supernatant, it was found that spermidine or spermine, at physiological concentrations, sufficed for the transport of the precursor of ornithine carbamoyltransferase. The activity of the postmitochondrial supernatant was inactivated by trypsin and slightly decreased by RNase treatment; it was not lost by dialysis or by heating at 100 degrees C.
Miralles
V J
VJ
Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Centro Associado del CSIC, Spain.
González-Bosch
C
C
Marcote
M J
MJ
Hernández-Yago
J
J
Grisolía
S
S
D000818:Animals; D002474:Cell-Free System; D003600:Cytosol / Q000378:metabolism; D004792:Enzyme Precursors / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology*; D009954:Ornithine Carbamoyltransferase / Q000096:biosynthesis* / Q000235:genetics; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism
D004792:Enzyme Precursors; D009954:Ornithine Carbamoyltransferase