Iron-sulfur centers involved in methanogenic electron transfer in Methanobacterium thermoautotrophicum (delta-H).
Utilizing a subcellular particulate preparation from Methanobacterium thermoautotrophicum (delta-H) which contains all detectable methanogenic electron transfer activity, we present the results of the effects of the anaerobic addition of oxidized factor F420 and of methyl coenzyme M plus ATP on the EPR signals from reduced iron-sulfur centers and a rapidly-relaxing radical species. Based on these results, we report the existence of a minimum of three iron-sulfur centers which are capable of donating electrons to these cofactors.
Rogers
K R
KR
Department of Chemistry and Biochemistry, Utah State University, Logan 84322-0300.
Gillies
K
K
Lancaster
J R
JR
D000693:Anaerobiosis; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D019605:Euryarchaeota / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D015080:Mesna / Q000378:metabolism; D008667:Metalloproteins / Q000378:metabolism*; D008872:Microwaves; D010084:Oxidation-Reduction; D012256:Riboflavin / Q000031:analogs & derivatives / Q000378:metabolism
D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D005182:Flavin-Adenine Dinucleotide; C015084:coenzyme F420; D005949:Glucose Oxidase; D015080:Mesna; D012256:Riboflavin