A chemically defined medium for the culture of mature oligodendrocytes.
A new chemically defined medium consisting of equal parts of Dulbecco modified Eagle's and Ham's F-12 media supplemented with insulin, sodium selenite, putrescine, and D+ galactose, which allows the long-term survival of mature oligodendrocyte pure cultures, is described. Immunohistochemical staining has shown that over 90% of the cells become positive for myelin proteins shortly following subculture. Contaminating astrocytes (2%) do not survive in this medium. Biochemical data have indicated that these purified oligodendrocytes express 2'3'-cyclic nucleotide 3'-phosphohydrolase and UDP-galactose ceramide galactosyltransferase activities. Electron microscopical examination revealed that the oligodendrocytes were mostly of medium-dark type and appeared to be identical to cells cultured in serum-containing medium. The ability to maintain pure oligodendrocyte cultures in such a defined medium will allow investigations concerning exogenous and endogenous factors involved in oligodendrocyte metabolism.
Espinosa de los Monteros
A
A
Centre de Neurochimie du CNRS, Strasbourg, France.
Roussel
G
G
Neskovic
N M
NM
Nussbaum
J L
JL
D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods*; D005700:Galactosyltransferases / Q000378:metabolism; D051147:Ganglioside Galactosyltransferase; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases; D013997:Time Factors
D003470:Culture Media; D005904:Glial Fibrillary Acidic Protein; D005700:Galactosyltransferases; D051147:Ganglioside Galactosyltransferase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase