Transformation of human fibroblasts by ionizing radiation, a chemical carcinogen, or simian virus 40 correlates with an increase in susceptibility to the autonomous parvoviruses H-1 virus and minute virus of mice.
Morphologically altered and established human fibroblasts, obtained either by 60Co gamma irradiation, treatment with the carcinogen 4-nitroquinoline 1-oxide, or simian virus 40 (SV40) infection, were compared with their normal finite-life parental strains for susceptibility to the autonomous parvoviruses H-1 virus and the prototype strain of minute virus of mice (MVMp). All transformed cells suffered greater virus-induced killing than their untransformed progenitors. The cytotoxic effect of H-1 virus was more severe than that of MVMp. Moreover, the level of viral DNA replication was much (10- to 85-fold) enhanced in the transformants compared with their untransformed parent cells. Thus, in this system, cell transformation appears to correlate with an increase in both DNA amplification and cytotoxicity of the parvoviruses. However, the accumulation of parvovirus DNA in the transformants was not always accompanied by the production of infectious virus. Like in vitro-transformed fibroblasts, a fibrosarcoma-derived cell line was sensitive to the killing effect of both H-1 virus and MVMp and amplified viral DNA to high extents. The results indicate that oncogenic transformation can be included among cellular states which modulate permissiveness to parvoviruses under defined growth conditions.
Cornelis
J J
JJ
Laboratory of Molecular Oncology, Institut National de la Santé et de la Recherche Meédicale U 186, Lille, France.
Becquart
P
P
Duponchel
N
N
Salomé
N
N
Avalosse
B L
BL
Namba
M
M
Rommelaere
J
J
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D004198:Disease Susceptibility; D005347:Fibroblasts / Q000382:microbiology / Q000528:radiation effects*; D005784:Gene Amplification; D006801:Humans; D008915:Minute Virus of Mice / Q000235:genetics*; D010321:Parvoviridae / Q000235:genetics*; D013539:Simian virus 40
D004279:DNA, Viral; D015112:4-Nitroquinoline-1-oxide