Phosphatidylinositol-4,5-bisphosphate may antecede diacylglycerol as activator of protein kinase C.
Phosphatidylserine/calcium-dependent protein kinase C (PKC) from rat brain is activated fifty times more efficiently by phosphatidylinositol-4,5-bisphosphate (PIP2) (Kapp = 0.04 mole% in Triton-lipid micelles) than by diacylglycerol (DG) (Kapp = 2 mole%). Both effector lipids appear to bind to the same site but PIP2 may confer a narrower substrate specificity on the kinase. DG, which together with inositol trisphosphate (IP3) is generated by hydrolysis from PIP2 after cell stimulation, has been considered the natural activator of the kinase but it is likely to be anteceded in this function by PIP2; DG may perhaps retain the function of a back-up activator. The lack of PKC-activation by phosphatidylinositol (PI) or phosphatidylinositol-4-phosphate (PIP) opens the possibility that the Inositide Shuttle, PI reversible PIP reversible PIP2, has a role in controlling the activity of the kinase.
Chauhan
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VP
New York State Office of Mental Retardation and Developmental Disabilities, Staten Island 10314.
Brockerhoff
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D000818:Animals; D002118:Calcium / Q000502:physiology; D004075:Diglycerides / Q000502:physiology*; D004789:Enzyme Activation; D005989:Glycerides / Q000502:physiology*; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000502:physiology*; D010718:Phosphatidylserines / Q000502:physiology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
D004075:Diglycerides; D005989:Glycerides; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; C037178:phosphatidylinositol 4-phosphate; D011493:Protein Kinase C; D002118:Calcium