Strontium-phosphate hydroxyapatite high-performance liquid chromatography.
High-performance liquid chromatography using spherical aggregates of strontium-phosphate hydroxyapatite(SrHA) micro-crystals as adsorbent has been developed; preliminary performance tests were carried out by using several types of protein. It can be deduced that, in parallel with the case of usual calcium-phosphate hydroxyapatite(CaHA), with SrHA also, two types of effective surface, vector a (or vector b) and vector c surfaces, appear on the crystal: the same protein molecular generally shows slightly different chromatographic behaviors between the CaHA and the SrHA packed column. Combining the SrHA and the CaHA packed column would lead to an efficient fractionation of a particular molecule from an assembly of molecules with subtle structural differences from one another.
Kawasaki
T
T
Chromatographic Research Laboratory, Koken Co. Ltd., Tokyo, Japan.
Niikura
M
M
Takahashi
S
S
Kobayashi
W
W
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017886:Durapatite; D006882:Hydroxyapatites; D007202:Indicators and Reagents; D010710:Phosphates; D011506:Proteins / Q000302:isolation & purification*; D013324:Strontium
D006882:Hydroxyapatites; D007202:Indicators and Reagents; D010710:Phosphates; D011506:Proteins; D017886:Durapatite; C052774:strontium phosphate; D013324:Strontium