Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN.
We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always greater than or equal to 99% with 90% recovery of the monocytes from the mononuclear cells.
Vissers
M C
MC
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0602.
Jester
S A
SA
Fantone
J C
JC
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D003102:Colloids; D003973:Diatrizoate; D005362:Ficoll; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes; D009000:Monocytes; D011134:Polysaccharides; D012822:Silicon Dioxide
D003102:Colloids; D011134:Polysaccharides; D003973:Diatrizoate; D005362:Ficoll; D012822:Silicon Dioxide