Demonstration of Chromogranin A in human neuroendocrine cell lines by immunohistology and immunoassay.
We have used immunohistology and radioimmunoassay procedures to study Chromogranin A (CgA) in human neuroendocrine tumor cell lines, especially small cell lung cancers (SCLC). By immunohistology, CgA could be detected in 11 of 18 classical SCLC cell lines, in a medullary thyroid carcinoma (MTC) cell line, and in only one of 13 variant- or non-SCLC cell lines. By radioimmunoassay, CgA could be detected in the cells and culture media of all of the classical SCLC cell lines tested. Many of the classical SCLC cell lines also produced calcitonin (CT). These studies demonstrate that CgA production is a common feature of SCLC cell lines, especially those with neuroendocrine characteristics.
Deftos
L J
LJ
Department of Medicine, University of California, La Jolla 92161.
Linnoila
R I
RI
Carney
D N
DN
Burton
D W
DW
Leong
S S
SS
O'Connor
D T
DT
Murray
S S
SS
Gazdar
A F
AF
D002116:Calcitonin / Q000032:analysis; D002276:Carcinoid Tumor / Q000032:analysis; D018288:Carcinoma, Small Cell / Q000032:analysis*; D002460:Cell Line; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011863:Radioimmunoassay; D013964:Thyroid Neoplasms / Q000032:analysis
C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D002116:Calcitonin