Solubilization of a large molecular weight form of the rat LHRH receptor.
The LHRH receptor has been solubilized from male rat anterior pituitary glands, using the zwitterionic detergent 3-((3-cholamidopropyl)-dimethylammonio)-1-propanesulphonate in the presence of a high concentration of sodium chloride. This method gave high yields (up to greater than 70%) of the LHRH-binding site from the membrane preparation. Ligand binding studies using LHRH analogues were carried out to determine dissociation constants for LHRH receptors both in situ in the membrane preparation and for solubilized LHRH receptors. For all the analogues the binding characteristics were similar in both preparations, suggesting that the solubilization procedure left the LHRH receptor undenatured. Gel filtration revealed an apparent molecular weight for the LHRH receptor of 100,000-160,000, with the mean value being approximately twice that found by others using sodium dodecyl sulphate-polyacrylamide gel electrophoretic techniques. The results indicate that the LHRH receptor probably exists in gonadotroph membranes as a large complex of more than one subunit.
Ogier
S A
SA
MRC Brain Metabolism Unit, University Department of Pharmacology, Edinburgh.
Mitchell
R
R
Fink
G
G
D000818:Animals; D007987:Gonadotropin-Releasing Hormone; D008297:Male; D008722:Methods; D008970:Molecular Weight; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000032:analysis*; D012995:Solubility
D011956:Receptors, Cell Surface; D007987:Gonadotropin-Releasing Hormone