Radiation inactivation analysis of fusion and hemolysis by vesicular stomatitis virus.
Radiation inactivation analysis was used to determine the size of the functional unit responsible for fusion of vesicular stomatitis virus (VSV) with cardiolipin or phosphatidylcholine-phosphatidylethanolamine (1:1) liposomes, and for VSV-induced hemolysis. When radiation-insensitive background values were subtracted, the calculated functional units for all three activities were similar, ranging from 866 to 957 kDa, equivalent to about 15 G protein molecules. This is in striking contrast to results of similar studies with influenza and Sendai viruses, in which the functional unit corresponded in size to a single fusion protein monomer, and suggests that VSV fusion may occur by a different mechanism.
Bundo-Morita
K
K
Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854-5635.
Gibson
S
S
Lenard
J
J
D006461:Hemolysis; D008081:Liposomes; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000502:physiology / Q000528:radiation effects*; D008970:Molecular Weight; D009975:Orthomyxoviridae / Q000528:radiation effects; D010222:Parainfluenza Virus 1, Human / Q000528:radiation effects; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology* / Q000528:radiation effects; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins / Q000502:physiology / Q000528:radiation effects*
C028391:G protein, vesicular stomatitis virus; D008081:Liposomes; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins