Four phosphoproteins with common amino termini are encoded by human cytomegalovirus AD169.
In this report, we identify the proteins encoded by the 2.2-kilobase class of early transcripts arising from a region of the strain AD169 human cytomegalovirus genome (map units 0.682 to 0.713) which contains cell-related sequences. These transcripts, encoded by adjacent EcoRI fragments R and d, have a complex spliced structure with 5' and 3' coterminal ends. Antiserum directed against a synthetic 11-amino-acid peptide corresponding to the predicted amino terminus of the proteins was generated and found to immunoprecipitate four infected-cell proteins of 84, 50, 43, and 34 kilodaltons. These proteins were phosphorylated and were associated predominantly with the nuclei of infected cells. The 43-kilodalton protein was the most abundant of the four proteins, and its level of expression remained relatively constant throughout the infection. Expression of the other proteins increased as the infection progressed. Pulse-chase analysis failed to show a precursor-product relationship between any of the proteins. A comparison of the [35S]methionine-labeled tryptic peptide maps of the four proteins from infected cells and an in vitro-generated polypeptide derived from the putative first exon showed that all four infected-cell proteins were of viral origin and contained a common amino-terminal region.
Wright
D A
DA
Department of Biology, University of California, San Diego, La Jolla 92093.
Staprans
S I
SI
Spector
D H
DH
D000595:Amino Acid Sequence; D001483:Base Sequence; D002451:Cell Compartmentation; D003587:Cytomegalovirus / Q000235:genetics*; D005814:Genes, Viral; D006031:Glycosylation; D007158:Immunologic Techniques; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D010750:Phosphoproteins / Q000235:genetics*; D010766:Phosphorylation; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D014357:Trypsin; D014764:Viral Proteins / Q000235:genetics*
D010750:Phosphoproteins; D011498:Protein Precursors; D014764:Viral Proteins; D014357:Trypsin