DNA topoisomerase I from Leydig cells is modulated by luteinizing hormone and cyclic adenosine monophosphate.
The action of luteinizing hormone on topoisomerase I activity from rat Leydig cells was studied. Stimulation of the enzyme was observed after long-term (24 and 48 h) gonadotrophin treatment in in vivo experiments. No change could be detected for shorter times than 12 h using two different experimental approaches. Topoisomerase I was stimulated by cAMP in a whole cell extract in a phosphorylation-dependent manner. These results suggest that topoisomerase I could be a target for nuclear events induced by peptide hormone action.
Crespi
M D
MD
Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina.
Radicella
J P
JP
Baldi
A
A
Charreau
E H
EH
Calvo
J C
JC
D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D006062:Gonadotropins / Q000494:pharmacology; D066298:In Vitro Techniques; D047908:Intracellular Signaling Peptides and Proteins; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology*; D007986:Luteinizing Hormone / Q000502:physiology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000097:blood
D002352:Carrier Proteins; D006062:Gonadotropins; D047908:Intracellular Signaling Peptides and Proteins; C015482:protein kinase modulator; D013739:Testosterone; D007986:Luteinizing Hormone; D000242:Cyclic AMP; D004264:DNA Topoisomerases, Type I