Allograft tolerance induction in adult mice associated with functional deletion of specific CTL precursors.
Rejection of H-2 class I bm 1 mutant skin allografts by B6 recipient mice is mediated by a population of CD8+ anti-bm1 cytotoxic T-lymphocytes that produces and consumes its own T helper factor in response to bm1 skin allografts (dual function CTL). Previously we have demonstrated that transfusion of allogeneic lymphocytes across an H-2 class I disparity induces specific long-lasting skin allograft survival. We now show that intravenous injection of allogeneic spleen cells across the bm1 mutant disparity results in a temporary decrease of donor-reactive CTLp in the spleen of recipient mice, lasting for approximately five weeks. The sponge matrix allograft model was used to show that allograft tolerance is caused by a specific functional clonal deletion of CTLp within the allograft. We propose that dual function CTL are vetoed by donor T cells, resulting in skin allograft tolerance.
van Twuyver
E
E
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, University of Amsterdam.
Kast
W M
WM
Mooijaart
R J
RJ
Wilmink
J M
JM
Melief
C J
CJ
de Waal
L P
LP
D000818:Animals; D006085:Graft Survival; D006183:H-2 Antigens / Q000276:immunology; D007108:Immune Tolerance; D007958:Leukocyte Count; D017710:Lymphocyte Transfusion; D051379:Mice; D008815:Mice, Inbred Strains; D016038:Skin Transplantation / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013997:Time Factors
D006183:H-2 Antigens