"A" subunit of factor XIII is present on bovine platelet membrane and mediates collagen-induced platelet activation.
The Fab fragment of a polyclonal antibody against platelet factor XIII inhibited the collagen-induced platelet aggregation in a dose-dependent manner. This inhibitory effect was specific for collagen, and it had no effect on arachidonic acid-, ADP-, and serotonin-induced aggregations. This finding strengthens our notion that platelet factor XIII is involved in collagen-induced platelet aggregation. (Saito, Y., Imada, T., Takagi, J., Kikuchi, T. and Inada, Y. J. Biol. Chem. 261, 1355-1358, 1986). We have investigated membrane localization of bovine platelet factor XIII using immunological techniques. Immunofluorescent visualization revealed that the factor XIII was expressed on the surface of non-permeabilized bovine platelets, where we detected neither lactate dehydrogenase, a cytoplasmic enzyme marker, nor B subunit of factor XIII, which is present in plasma. Cell surface iodination and immunoprecipitation also confirmed that it existed on the surface of platelets.
Kasahara
K
K
Laboratory of Biological Chemistry, Tokyo Institute of Technology, Japan.
Takagi
J
J
Sekiya
F
F
Inada
Y
Y
Saito
Y
Y
D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D002417:Cattle; D003094:Collagen / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005176:Factor XIII / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D010974:Platelet Aggregation / Q000187:drug effects*; D011956:Receptors, Cell Surface / Q000032:analysis*; D038981:Receptors, Collagen
D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D003094:Collagen; D005176:Factor XIII