[Study of the structure of phage lambda operator OR1 using two-dimensional 1H-NMR-spectroscopy].
NOESY spectroscopy at 500 HMz was employed to assign resonances of nonexchangeable protons in the 1H NMR spectrum of the synthesized 17 base pair duplex of deoxyribonucleotides comprising the OR1 operator, one of the specific binding sites for the bacteriophage lambda cro repressor. A pure absorption spectrum that was obtained by the phase sensitive detection technique allowed to perform a resonance assignment of base and deoxyribose protons, excepting H-5'- and H-5"-protons, on the basis of a single NOESY experiment, mixing time 200 ms. The sequential assignment strategy for 2D NMR spectra of oligonucleotides was used for this purpose. The data obtained and the previous results on OR3 are discussed in the view of the conformation of operators in solution. It is shown that both duplexes exist in the DNA B-form with its intrinsic anti conformation of the nucleotides. Conformation of DNA segments with identical primary structures are similar, and local deviations from the classical B-form are determined by a nucleotide sequence.
Kirpichnikov
M P
MP
Kurochkin
A V
AV
Skriabin
K G
KG
Chernov
B K
BK
Baev
A A
AA
D010582:Bacteriophage lambda / Q000032:analysis / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D009875:Operator Regions, Genetic; D011522:Protons
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D011522:Protons
Izuchenie struktury operatora OR1 bakteriofaga lambda metodom dvumernoĭ 1H-IaMR-spektroskopii.