An unusual alteration in c-myc in tissue from a primary breast carcinoma.
We have identified a rearranged c-myc gene in DNA from tumour tissue from a patient with an aggressive carcinoma of the breast. Analysis of this rearranged gene isolated from a size-fractionated genomic library revealed that a deletion had occurred within exon 3. This deletion, of approximately 5 kb, interrupts the coding region of exon 3, and would result in a truncated myc protein with an altered C-terminus. Sequence analysis of the rearranged c-myc gene and the sequence downstream of c-myc which is involved in the deletion has shown precisely where the breakpoint has occurred in both sequences and reveals a short region of homology which could perhaps permit illegitimate recombination between the two sequences.
Varley
J M
JM
ICI/University Joint Laboratory, University of Leicester, UK.
Wainwright
A M
AM
Brammar
W J
WJ
D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D011995:Recombination, Genetic
D011518:Proto-Oncogene Proteins; D004262:DNA Restriction Enzymes