title: cGMP phosphodiesterase of retinal rods is regulated by two inhibitory subunits.
abstract: The cGMP phosphodiesterase (PDE) of cattle retinal rod outer segments comprises three types of subunits: the two heavy catalytic ones, PDE alpha and PDE beta, each around 85 kDa, and the light inhibitory one, PDE gamma or I (11 kDa). The relative stoichiometry is usually assumed to be 1:1:1. PDE activation in the visual transduction cascade results from removal of the inhibitor by the alpha subunit of transducin (T alpha). The stoichiometric complex T alpha-I, separated from activated PDE, has been isolated and characterized. Analyzing now the activated PDE, we find that it still contains some inhibitor and is resolvable into two species, one with 50% of the inhibitor content of the native enzyme and the other totally devoid of it. The same two species are observed upon activation of PDE by very short tryptic proteolysis, which specifically degrades the inhibitor. This leads us to conclude that the composition of the native enzyme is PDE alpha beta-I2. The two inhibitory subunits are differentially bound, sequentially removable, and exchangeable between the native complex PDE alpha beta-I2 and the fully active PDE alpha beta. The possibility of this exchange precludes as yet an unambiguous estimate of the actual activity of the intermediate complex PDE alpha beta-I. The differential binding and the exchangeability of the inhibitors raises the possibility of a fast, diffusion controlled, switch-off mechanism of PDE activity after a flash, which would shortcut the inactivation resulting from the slow GTPase rate of transducin.
authors.affiliation: Laboratoire de Biophysique Moléculaire et Cellulaire, Unité Associée 520 du Centre National de la Recherche Scientifique, DRF/CENG, Grenoble, France.
authors.forename: P
authors.initials: P
authors.lastname: Deterre
authors.forename: J
authors.initials: J
authors.lastname: Bigay
authors.forename: F
authors.initials: F
authors.lastname: Forquet
authors.forename: M
authors.initials: M
authors.lastname: Robert
authors.forename: M
authors.initials: M
authors.lastname: Chabre
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004789:Enzyme Activation; D006160:Guanosine Triphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000502:physiology; D010726:Phosphodiesterase Inhibitors; D010786:Photoreceptor Cells / Q000201:enzymology*; D012374:Rod Cell Outer Segment / Q000201:enzymology*; D015293:Transducin
chemical_list: D046911:Macromolecular Substances; D008565:Membrane Proteins; D010726:Phosphodiesterase Inhibitors; D006160:Guanosine Triphosphate; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D015293:Transducin