title: An improved procedure for the rapid one-step-cloning of full-length viroid cDNA.
abstract: The efficiency of viroid cloning can be increased by three to four orders of magnitude when the synthesis of viroid cDNA is primed in such a way that it carries identical sticky ends on both termini and when the multi-ion transformation is applied.
authors.affiliation: Max-Planck-Institut für Biochemie, Abteilung Viroidforschung, Martinsried bei München, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Puchta
authors.forename: H L
authors.initials: HL
authors.lastname: Sänger
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism; D004526:Efficiency; D004926:Escherichia coli / Q000235:genetics; D014170:Transformation, Genetic; D014772:Viroids / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes