Reduced cAMP-dependent phosphorylation in striatum and nucleus accumbens of aged rats: evidence of an altered functioning of D1 dopaminoceptive neurons.
Cyclic AMP-dependent phosphorylation was measured in particulate and in cytosolic fractions of two brain dopaminergic areas, i.e., striatum and nucleus accumbens, of young (3 months) and old (24 months) rats. A reduced cAMP-stimulated 32P incorporation was observed in several protein bands in both brain areas in the aged group. In the soluble fraction the reduced phosphorylation of DARPP-32, which is specific for dopaminoceptive neurons bearing D-1 receptors, may be of particular relevance in the mechanisms of age-related changes in dopaminergic transmission.
Govoni
S
S
Institute of Pharmacological Sciences, University of Milan, Italy.
Rius
R A
RA
Battaini
F
F
Trabucchi
M
M
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001923:Brain Chemistry; D003342:Corpus Striatum / Q000032:analysis / Q000378:metabolism*; D000242:Cyclic AMP / Q000032:analysis / Q000378:metabolism*; D003600:Cytosol / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008970:Molecular Weight; D009474:Neurons / Q000032:analysis / Q000378:metabolism*; D009714:Nucleus Accumbens / Q000032:analysis / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000032:analysis / Q000378:metabolism*; D017447:Receptors, Dopamine D1; D012686:Septal Nuclei / Q000378:metabolism*
D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D000242:Cyclic AMP; D011494:Protein Kinases