Translation in mammalian cells of a gene linked to the poliovirus 5' noncoding region.
The central portion (region P) of the 742-nucleotide noncoding 5' end of poliovirus allows the RNA to initiate protein synthesis in the absence of the usual 5' 7-methylguanosine capping group. Poliovirus 5' noncoding region was fused to a reporter gene and transfected into cells. There was extensive augmentation of the expression of this gene by poliovirus-mediated inhibition of cap-dependent protein synthesis. That the construct initiated in a cap-independent manner was verified through in vitro experiments. Small lesions throughout region P blocked its initiation function, implying that a coherent functional unit, hundreds of nucleotides long, is responsible for cap-independent initiation by poliovirus RNA.
Trono
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D
Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Pelletier
J
J
Sonenberg
N
N
Baltimore
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D
D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D015500:Chloramphenicol O-Acetyltransferase; D002522:Chlorocebus aethiops; D004252:DNA Mutational Analysis; D004274:DNA, Recombinant; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012315:RNA Caps; D012333:RNA, Messenger / Q000235:genetics
D004274:DNA, Recombinant; D012315:RNA Caps; D012333:RNA, Messenger; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase