Comparison of cytomegalovirus antibodies by means of CF, ELISA and immunoblotting techniques.
Antibody to human cytomegalovirus in sera of immune blood donors reacted with 12 viral polypeptides of molecular weight 200.000. 150.000, 65.000, 48.000, 34,000. 25.000, 23.000, 21.000 and 18.000. Development of CMV infection in renal transplant recipients resulted in increase of antibody reacting with various CMV polypeptides as well as in intensity of this reaction. The highly comparable results for CMV antibody detecting were obtained with immunoblotting and ELISA techniques.
Jankowski
M
M
Department of Virology, National Institute of Hygiene, Warsaw.
Styczyński
P
P
D000914:Antibodies, Viral / Q000032:analysis* / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007158:Immunologic Techniques; D016030:Kidney Transplantation; D008970:Molecular Weight; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins