title: Expression of nerve growth factor receptor during human peripheral nerve development.
abstract: The expression of NGF receptors on human Schwann cells during development and myelination and in culture was analyzed using a murine monoclonal antibody to human NGF receptor. Nonmyelinated femoral nerves from 13- to 14-week fetuses stained strongly for NGF receptor, whereas tissues from later stages of development showed a decrease in the staining intensity. These changes correlated with the initiation of myelination (17-19 weeks), as observed by phase-contrast and electron microscopy, and the reactivity with monoclonal antibody 4C5, a marker of mature Schwann cells. In adult nerves, only the perineurium and few endoneurial cells were stained with anti-NGF receptor antibody. Cultured human fetal Schwann cells were positive for NGF receptor by immunofluorescence irregardless of donor age or length of time in culture. The decreased staining of NGF receptor with nerve maturation may reflect a dependence of antigen expression on Schwann cell differentiation and/or neuron-Schwann cell interaction.
authors.affiliation: Department of Neurology, Dino Ferrari Center for Neuromuscular Diseases, University of Milan, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Scarpini
authors.forename: A H
authors.initials: AH
authors.lastname: Ross
authors.forename: J L
authors.initials: JL
authors.lastname: Rosen
authors.forename: M J
authors.initials: MJ
authors.lastname: Brown
authors.forename: A
authors.initials: A
authors.lastname: Rostami
authors.forename: H
authors.initials: H
authors.lastname: Koprowski
authors.forename: R P
authors.initials: RP
authors.lastname: Lisak
mesh_terms: D002478:Cells, Cultured; D005865:Gestational Age; D006801:Humans; D007124:Immunoenzyme Techniques; D009414:Nerve Growth Factors / Q000378:metabolism; D010525:Peripheral Nerves / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000276:immunology; D017475:Receptors, Nerve Growth Factor; D012583:Schwann Cells / Q000378:metabolism*
chemical_list: D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor