Characterization of phosphoglycerate mutase isoenzymes from free dissected facial processes.
To characterize the three phosphoglycerate mutase (PGM) isoenzymes present in rat facial processes (types MM, BB, and MB), their sensitivity to reagents of the sulfhydryl groups and to heat treatment has been studied. Type BB PGM was not affected by the -SH group reagents; type MB PGM was inhibited about 50%, and type MM PGM was fully inhibited. Type MB PGM showed a greater heat lability than type MM PGM. There was a developmental change from type BB PGM from the 9th embryonic day to isoenzymes MB and MM on the 15th embryonic day. Isoenzyme development was first seen in mandibular processes, followed by maxillary, lateral nasal, and medial nasal processes.
Granström
G
G
Department of Histology, University of Gothenburg, Sweden.
Mångs
H
H
Nilsson
L P
LP
Zellin
G
G
D000818:Animals; D003720:Densitometry; D005260:Female; D006358:Hot Temperature; D007525:Isoelectric Focusing / Q000379:methods; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D008334:Mandible / Q000196:embryology / Q000201:enzymology*; D008437:Maxilla / Q000196:embryology / Q000201:enzymology*; D009295:Nasal Bone / Q000196:embryology / Q000201:enzymology*; D010736:Phosphoglycerate Mutase / Q000032:analysis*; D010770:Phosphotransferases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D007527:Isoenzymes; D013439:Sulfhydryl Reagents; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase