Acute effects of a superoxide radical-generating system on DNA double-strand stability in Chinese hamster ovary cells. Determination by a modified fluorometric procedure.
The rate of oxyradical generation by a xanthine oxidase-xanthine system to acutely cause DNA strand breakage in Chinese hamster ovary cells was studied in a phosphate-buffered saline system. DNA strand breakage, measured by a fluorometric procedure, was found to increase curvilinearly as a function of oxyradical generation. Results of studying the ability of 5 mM mannitol, 10 mM dimethylthiourea, 300 micrograms superoxide dismutase/ml, or 1 mg catalase/ml to interfere with DNA damage at a high rate of oxyradical production best supported a hydrogen peroxide-promoted mechanism for DNA breakage.
Hall
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AH
Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333.
Eanes
R Z
RZ
Waymack
P P
PP
Patterson
R M
RM
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005470:Fluorometry; D005609:Free Radicals; D008353:Mannitol / Q000494:pharmacology; D010053:Ovary; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000096:biosynthesis*; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D005609:Free Radicals; D014970:Xanthines; D013481:Superoxides; D019820:Xanthine; D008353:Mannitol; C038983:1,3-dimethylthiourea; D004247:DNA; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D013890:Thiourea