Colloidal gold-labeled lipoprotein binding and internalization in adrenocortical cells in vitro.
Human (normal and adenomatous) and rat (normal) adrenocortical cells were incubated in vitro with colloidal gold labeled low-density (LDL-Au) and high-density (HDL-Au) lipoproteins, respectively, in order to visualize lipoprotein binding and internalization at an electron microscopic level. Both normal and adenomatous human adrenocortical cells accumulated LDL-Au by receptor-mediated endocytosis via coated pits, coated vesicles, noncoated vesicles, and lysosomes. HDL-Au was not internalized. In rat adrenocortical cells, both HDL-Au and to a lesser extent LDL-Au were internalized. It is concluded that LDL-Au and HDL-Au conjugates can be used to identify lipoprotein receptors and to follow lipoprotein internalization in adrenocortical cells.
Tóth
I E
IE
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest.
Szabó
D
D
Szalay
K S
KS
Gyévai
A
A
Szollár
L G
LG
Gláz
E
E
D000302:Adrenal Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000368:Aged; D000818:Animals; D003480:Cushing Syndrome / Q000378:metabolism; D005260:Female; D006048:Gold Colloid, Radioactive / Q000097:blood*; D006801:Humans; D006929:Hyperaldosteronism / Q000378:metabolism; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008854:Microscopy, Electron; D008875:Middle Aged; D010673:Pheochromocytoma / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains
D006048:Gold Colloid, Radioactive; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL