title: Crocidolite-induced lipid peroxidation. II. Role of antioxidants.
abstract: Asbestos fibers in vitro produce lipid peroxidation in rat lung microsomes. Butylated hydroxytoluene prevented this peroxidation. Ascorbate in low concentrations enhanced peroxidation of lipids but inhibited it at concentrations above 4 mmole/liter so that it partially protected membrane lipids from peroxidation produced by asbestos fibers. Reduced glutathione added to microsomes gave increased peroxidation at increased concentrations up to 20 mmol/liter. At 40 mmol/liter peroxidation was prevented. Glutathione had no obvious effect on the level of peroxidation produced by asbestos fibers. The 105,000g supernatant cell fraction added either with or without glutathione gave a decrease in the amount of lipid peroxidation produced by asbestos fibers. The protective action of these reducing agents suggests a possible use as prophylactic agents against the harmful effects of inhaled asbestos.
authors.affiliation: National Centre for Occupational Health, Johannesburg, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Gulumian
authors.forename: T A
authors.initials: TA
authors.lastname: Kilroe-Smith
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001194:Asbestos / Q000494:pharmacology*; D017638:Asbestos, Crocidolite; D001205:Ascorbic Acid / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D017638:Asbestos, Crocidolite; D001194:Asbestos; D002084:Butylated Hydroxytoluene; D005978:Glutathione; D001205:Ascorbic Acid