title: A stable genomic source of P element transposase in Drosophila melanogaster.
abstract: A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for "helper" plasmids in P element mediated transformation, and can be used to move transformed elements around the genome.
authors.affiliation: Laboratory of Genetics, University of Wisconsin, Madison 53706.
authors.forename: H M
authors.initials: HM
authors.lastname: Robertson
authors.forename: C R
authors.initials: CR
authors.lastname: Preston
authors.forename: R W
authors.initials: RW
authors.lastname: Phillis
authors.forename: D M
authors.initials: DM
authors.lastname: Johnson-Schlitz
authors.forename: W K
authors.initials: WK
authors.lastname: Benz
authors.forename: W R
authors.initials: WR
authors.lastname: Engels
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005796:Genes; D008297:Male; D009693:Nucleic Acid Hybridization; D009713:Nucleotidyltransferases / Q000235:genetics*; D014170:Transformation, Genetic; D019895:Transposases
chemical_list: D004251:DNA Transposable Elements; D009713:Nucleotidyltransferases; D019895:Transposases