Translation arrest of potato virus X RNA in Krebs-2 cell-free system: RNase H cleavage promoted by complementary oligodeoxynucleotides.
Translation arrest of genomic potato virus X (PVX) RNA promoted by complementary oligodeoxynucleotides in Krebs-2 cell-free system is described. 14-15 mer oligodeoxynucleotides complementary to the 5'-proximal cistron of PVX RNA were shown to induce specific truncation of the major non-structural polypeptide coded by PVX RNA. Evidence is presented that effective translational arrest of PVX RNA in the presence of complementary oligonucleotides results from the site-specific cleavage of RNA by endogenous RNase H intrinsic to the Krebs-2 extract. No similar translational arrest was found in the rabbit reticulocyte lysate cell-free system.
Miroshnichenko
N A
NA
Institute of Microbiology, Moscow, USSR.
Karpova
O V
OV
Morozov SYu
Rodionova
N P
NP
Atabekov
J G
JG
D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism; D002474:Cell-Free System; D004247:DNA / Q000235:genetics; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000494:pharmacology*; D010942:Plant Viruses / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D016914:Ribonuclease H; D014908:Triticum
D009711:Nucleotides; D012367:RNA, Viral; D004247:DNA; D004722:Endoribonucleases; D016914:Ribonuclease H