Plasmid DNA mediated transfer of the herpes simplex virus thymidine kinase gene to a new bromodeoxyuridine resistant variant of human primary lung carcinoma cells.
A human primary lung carcinoma cell line (HPL-R1) established from the tumor biopsy of a lung cancer patient, lacking in cytochrome P1-450 [aryl hydrocarbon (benzo[a]pyrene) hydroxylase (AHH)], was cloned and used to obtain variants deficient in the expression of thymidine-kinase via treatment with 5-bromo-2'-deoxyuridine, and selection for drug resistance phenotype. The variant cell line, precharacterized for thymidine kinase negative phenotype, was transfected with the thymidine kinase gene bearing p R-tk and px1-tk plasmids. Transfections from both the plasmids, demonstrated a frequency of 5.5 X 10(-5). The transfectants showed a 76-100% retention of the transferred phenotype. These data suggest that transfection in variant human cells can approach significant levels of stability observed with rodent cell recipients.
Manjunath
G S
GS
Department of Biological Sciences, University of Windsor, Ontario, Canada.
Dufresne
M J
MJ
D001973:Bromodeoxyuridine / Q000494:pharmacology*; D004351:Drug Resistance / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D008175:Lung Neoplasms / Q000201:enzymology*; D009154:Mutation; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D013937:Thymidine Kinase; D001973:Bromodeoxyuridine