title: Evidence for endonucleolytic cleavages in decay of lacZ and lacI mRNAs.
abstract: S1 nuclease mapping revealed lacZ mRNA molecules whose 5' and 3' ends were internal to the transcription start and consistent with cleavages at pyrimidine-adenosine bonds 20 to 50 nucleotides apart. With the net 5'-to-3' direction known, lacZ mRNA is probably degraded by sequential cleavages of naked mRNA at vulnerable sites exposed by transit of the last translating ribosome.
authors.affiliation: Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: M N
authors.initials: MN
authors.lastname: Subbarao
authors.forename: D
authors.initials: D
authors.lastname: Kennell
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D012333:RNA, Messenger; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases