Recombinant interleukin 1 alpha and beta prime human monocyte superoxide production but have no effect on chemotaxis and oxidative burst response of neutrophils.
We report that recombinant human IL 1 alpha and beta and the two synthetic short fragments 163-171 and 226-254 do not exhibit chemotactic activity for human peripheral blood neutrophils and monocytes. These products up to concentrations of 4 X 10(4) units/ml failed to show chemotactic activity. Furthermore, IL 1 alpha and beta failed to generate chemotactic factors from human serum. Recombinant IL 1 alpha, IL 1 beta, or IL 1 beta fragments 163-171 and 226-254 did not induce any superoxide response by monocytes or neutrophils. However, exposure of monocytes to recombinant IL 1 alpha or IL 1 beta resulted in enhanced generation of superoxide response following stimulation with PMA. No priming was observed in neutrophils. These results suggest that IL 1 alpha and beta are involved in regulation of monocyte oxidative burst response, but play no direct regulatory role on neutrophil function.
Kharazmi
A
A
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Nielsen
H
H
Bendtzen
K
K
D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
D007375:Interleukin-1; D011994:Recombinant Proteins; D013481:Superoxides