title: Species differences in the molecular characteristics of vasoactive-intestinal-peptide receptors in the pancreas from rat and guinea-pig.
abstract: 1. The receptors for vasoactive intestinal peptide (VIP) present in dispersed acini and membranes from rat and guinea-pig pancreas differed in selectivity pattern, i.e. in the displacement of [125I]iodo-VIP by parent peptides, as revealed by a VIP:secretin IC50 ratio at least ten times higher in rat than in guinea-pig preparations. The molecular properties of these VIP receptors were therefore investigated. 2. When comparing six succinimidyl ester cross-linkers, bis[2-(succinimidooxycarbonyloxy)ethyl]sulfone proved to be the most universal [125I]iodo-VIP cross-linker for all pancreatic preparations. 3. In intact rat acini the main labeled peptide had an Mr of 80,000, whereas the main labeled peptide in intact guinea-pig acini was a smear of Mr 160,000. In both rat and guinea-pig pancreatic membranes, the main labeled peptide ([125I]iodo-VIP-binding-protein complex) had an Mr of 66,000. In addition, variable proportions of an Mr-80,000 peptide and an Mr-83,000 peptide were visualized in, respectively, rat and guinea-pig membranes. The labeling of all peptides was suppressed by VIP and by GTP. Reducing conditions allowed only a better resolution, making the presence of intermolecular disulfide bridges unlikely. 4. Taking into account the Mr of VIP it is thus plausible that the main native Mr-77,000 VIP-binding site present in rat acini could be easily converted to an Mr-63,000 peptide during membrane preparation, while in guinea-pig acini Mr-80,000 and/or Mr-63,000 VIP-binding sites were often closely associated with another membrane component in the native state. These molecular differences between VIP receptors in intact rat and guinea-pig acini are in keeping with functional differences.
authors.affiliation: Department of Biochemistry and Nutrition, Medical School, Free University of Brussels, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Svoboda
authors.forename: P
authors.initials: P
authors.lastname: Poloczek
authors.forename: J
authors.initials: J
authors.lastname: Winand
authors.forename: P
authors.initials: P
authors.lastname: Robberecht
authors.forename: J
authors.initials: J
authors.lastname: Christophe
mesh_terms: D000818:Animals; D001665:Binding Sites; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D008099:Liver / Q000032:analysis*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D010084:Oxidation-Reduction; D010179:Pancreas / Q000032:analysis*; D051381:Rats; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis* / Q000187:drug effects / Q000502:physiology; D018005:Receptors, Vasoactive Intestinal Peptide; D013045:Species Specificity; D013388:Succinimides
chemical_list: D003432:Cross-Linking Reagents; D008565:Membrane Proteins; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D013388:Succinimides