title: Nuclear ribosomal RNA genes and algal phylogeny--the Chlamydomonas example.
abstract: The nuclear ribosomal RNA genes (rDNA) of Chlamydomonas reinhardtii, C. moewusii and C. eugametos were examined with restriction endonuclease fragment and direct rRNA sequencing analyses. These comparative molecular data confirm similarity between C. moewusii and C. eugametos, and dissimilarity between the strains and C. reinhardtii. For C. moewusii and C. eugametos, the fragment analysis of digests with 16 (six base pair recognition site) restriction endonucleases revealed either no or minor differences. These minor differences appear to be confined to length and site variation in the rapidly evolving intergenic spacer region of the algal rDNA repeat unit. In contrast, patterns of digests for C. reinhardtii were completely different from those of C. moewusii and C. eugametos for all enzymes tested. Over two regions of the 18S ribosomal RNA (spanning approx. 300 bases) in C. moewusii and C. eugametos, we observed three possible base substitutions and no insertion/deletion events. The same comparison between C. reinhardtii and C. moewusii (or C. eugametos) revealed 31 base substitutions and eight insertion/deletion events. Overall, the rDNA comparisons support the proposed conspecificity of C. moewusii and C. eugametos, as well as the hypothesis that intraspecific variation in the algal ribosomal RNA coding region is minimal and that comparisons of rDNA sequences at higher taxonomic levels can be useful indicators of algal phylogeny. The degree of difference in the sequences of the 18S coding region between C. reinhardtii and C. moewusii or C. eugametos is comparable to that between an angiosperm and Equisetum and may reflect an ancient divergence between two species in one algal genus.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biochemistry, College of Basic Sciences, Louisiana State University, Baton Rouge 70803.
authors.forename: E R
authors.initials: ER
authors.lastname: Jupe
authors.forename: R L
authors.initials: RL
authors.lastname: Chapman
authors.forename: E A
authors.initials: EA
authors.lastname: Zimmer
mesh_terms: D002467:Cell Nucleus / Q000502:physiology; D002696:Chlamydomonas / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004275:DNA, Ribosomal / Q000235:genetics; D056890:Eukaryota / Q000235:genetics*; D005796:Genes; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics*; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes