Characterization of erythropoietin receptor on erythropoietin-unresponsive mouse erythroleukemia cells.
A membrane receptor for erythropoietin was identified in various erythropoietin-unresponsive mouse erythroleukemia cells. Scatchard analyses of the binding of human 125I-labeled erythropoietin to T3C1-2-0, K-1, GM86 and 707 cells showed the presence of a single class of binding sites with apparent Kd values of 0.27-0.78 nM, which are slightly higher than those of erythropoietin-responsive cells. The number of binding sites varied from 110 to 930 per cell. Crosslinking of 125I-erythropoietin to its binding sites with disuccinimidyl suberate revealed the existence of a single binding protein with molecular mass of 63 kDa. No binding sites with higher molecular mass, as observed in erythropoietin-responsive cells, were detected, nor was any specific binding observed to the non-erythroid hematopoietic cell or to the human erythroleukemia cells examined.
Todokoro
K
K
Tsukuba Life Science Center, Institute of Physical and Chemical Research, Ibaraki, Japan.
Kanazawa
S
S
Amanuma
H
H
Ikawa
Y
Y
D000818:Animals; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004921:Erythropoietin / Q000378:metabolism* / Q000494:pharmacology; D006412:Hematopoietic Stem Cells; D006801:Humans; D007457:Iodine Radioisotopes; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins / Q000378:metabolism; D013388:Succinimides; D014407:Tumor Cells, Cultured
D003432:Cross-Linking Reagents; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins; D013388:Succinimides; D004921:Erythropoietin; C019358:disuccinimidyl suberate