Chromosome-mediated transfer of the murine Na,K-ATPase alpha subunit confers ouabain resistance.
We transferred murine NIH 3T3 metaphase chromosomes into monkey CV-1 cells to investigate the different ouabain sensitivities of rodent and primate cells. In 16 ouabain-resistant transferents, the mouse Na,K-ATPase alpha 1 subunit gene was detected, suggesting that structural differences between the rodent and primate alpha 1 subunits determine the different ouabain sensitivities.
Fallows
D
D
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Kent
R B
RB
Nelson
D L
DL
Emanuel
J R
JR
Levenson
R
R
Housman
D E
DE
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000502:physiology*; D046911:Macromolecular Substances; D051379:Mice; D008815:Mice, Inbred Strains; D010042:Ouabain / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D014170:Transformation, Genetic
D046911:Macromolecular Substances; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase