Opsonin-dependent stimulation of bovine neutrophil oxidative metabolism by Brucella abortus.
Nonopsonized Brucella abortus and bacteria treated with fresh antiserum, heat-inactivated antiserum, or normal bovine serum were evaluated for their ability to stimulate production of superoxide anion and myeloperoxidase-mediated iodination by neutrophils from cattle. Brucella abortus opsonized with fresh antiserum or heat-inactivated antiserum stimulated production of approximately 3 nmol of O2-/10(6) neutrophils/30 min. Similarly treated bacteria also stimulated the binding of approximately 4.3 nmol of NaI/10(7) neutrophils/h to protein. Significant (P less than 0.05) production of O2- and iodination activity by neutrophils were not stimulated by nonopsonized bacteria or organisms treated with normal bovine serum. Seemingly, B abortus stimulated oxidative metabolism in bovine neutrophils; however, the stimulation was dependent on the presence of bacterial associated opsonins.
Canning
P C
PC
National Animal Disease Center, USDA, Ames, IA 50010.
Deyoe
B L
BL
Roth
J A
JA
D000818:Animals; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D003168:Complement Fixation Tests; D007106:Immune Sera / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009895:Opsonin Proteins / Q000276:immunology*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D015054:Zymosan / Q000276:immunology
D007106:Immune Sera; D009895:Opsonin Proteins; D013481:Superoxides; D015054:Zymosan; D009195:Peroxidase