Receptors for recombinant erythropoietin in human bone marrow cells.
Human recombinant erythropoietin, labelled with 125I, was saturably bound to high affinity receptors, ka = 1.5 X 10(9) M-1, of bone marrow cells from 10 patients with various haematological disorders. Binding of labelled erythropoietin was specific, with less than 0.01% cross-reactivities of TSH, hCG and renin substrate (angiotensinogen). Binding capacity averaged 3 X 10(-14) moles/10(6) cells. Binding was maximal within 30 min at 37 degrees C. Low binding, less than 15% of that to bone marrow cells, was found to peripheral blood buffy coat, while erythrocytes and BALL cells did not bind labelled erythropoietin. Autoradiography showed binding of label confined to 4-5% of the bone marrow cells.
Rosenlöf
K
K
Minerva Institute for Medical Research, Helsinki, Finland.
Fyhrquist
F
F
Pekonen
F
F
D000328:Adult; D000368:Aged; D001345:Autoradiography; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D005260:Female; D006402:Hematologic Diseases / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017467:Receptors, Erythropoietin
D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin