Demonstration of beta-adrenergic receptors in rat nasal glands by an in vitro autoradiographic technique.
The distribution of beta-adrenergic receptors in rat nasal glands has been investigated with the use of an in vitro autoradiographic technique. Radioligand binding studies indicated that [125I]cyanopindolol binds specifically to beta-adrenergic receptors in cryostat sections of these glands. Autoradiograms generated after incubation with 0.02 nM [125I]cyanopindolol and dipping in nuclear K2 emulsion, showed specific labelling of the striated excretory ducts. These in vitro observations suggest a sympathetic control of the ion and water content of the glandular secretion.
van Megen
Y J
YJ
Department of Otorhinolaryngology, University of Nijmegen, The Netherlands.
van Ratingen
C J
CJ
Klaassen
A B
AB
Rodrigues de Miranda
J F
JF
van Ginneken
C A
CA
van den Broek
P
P
D000320:Adrenergic Fibers / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography / Q000379:methods*; D066298:In Vitro Techniques; D009297:Nasal Mucosa / Q000294:innervation*; D010869:Pindolol / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; C047975:cyanopindolol; D010869:Pindolol