title: Metal complexes of luteinizing hormone-releasing hormone (LHRH). potentiometric and spectroscopic studies.
abstract: The results are reported of a potentiometric and spectroscopic study of the H+, Cu2+, and Ni2+ complexes of luteinizing hormone-releasing hormone (LHRH, HL) at 25 degrees C and an ionic strength 0.10 mol dm-3 (KNO3), since there is much evidence that the in vivo release of LHRH is influenced by the concentration of copper ions. With Cu2+ the hormone has been shown to behave similarly to the thyrotropin releasing factor, forming a very stable [CuH-1L] complex involving coordination of three nitrogen donors: the Nim atom of the imidazole side chain and the two amido-N atoms of the pyroglutamylhistidyl unit. With Ni2+, coordination proceeds differently to give four nitrogen coordination.
authors.affiliation: Institute of Chemistry, University of Wroclaw, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Gerega
authors.forename: H
authors.initials: H
authors.lastname: Kozlowski
authors.forename: E
authors.initials: E
authors.lastname: Masiukiewicz
authors.forename: L D
authors.initials: LD
authors.lastname: Pettit
authors.forename: S
authors.initials: S
authors.lastname: Pyburn
authors.forename: B
authors.initials: B
authors.lastname: Rzeszotarska
mesh_terms: D002942:Circular Dichroism; D003300:Copper; D004578:Electron Spin Resonance Spectroscopy; D007987:Gonadotropin-Releasing Hormone; D009532:Nickel; D009994:Osmolar Concentration; D011199:Potentiometry; D011487:Protein Conformation; D011522:Protons; D013053:Spectrophotometry
chemical_list: D011522:Protons; D007987:Gonadotropin-Releasing Hormone; D003300:Copper; D009532:Nickel