Expression of tobacco mosaic virus RNA in transgenic plants.
Tobacco mosaic virus (TMV) is a message-sense, single-stranded RNA virus that infects many Solanaceae plants. A full-length cDNA copy of TMV genomic RNA was constructed and introduced into the genomic DNA of tobacco plants using a disarmed Ti plasmid vector. Transformed plants showed typical symptoms of TMV infection, and their leaves contained infectious TMV particles. This is the first example of the expression of RNA virus genomic RNAs in plants.
Yamaya
J
J
Plant Laboratory, Kirin Brewery Co., Tochigi, Japan.
Yoshioka
M
M
Meshi
T
T
Okada
Y
Y
Ohno
T
T
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics* / Q000382:microbiology; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014158:Transcription, Genetic
D012367:RNA, Viral; D004262:DNA Restriction Enzymes