The 5'-terminal guanylate of chloroplast histidine tRNA is encoded in its gene.
Among tRNA species histidine tRNAs possess the unique feature of having an additional nucleotide, a guanylate, at their 5'-end. In prokaryotes this G is encoded in the gene sequence and retained in the mature tRNA as result of an unusual cleavage by RNase P (Orellana, O., Cooley, L., and Söll, D. (1986) Mol. Cell. Biol. 6, 525-529), while in eukaryotes it is added post-transcriptionally by a special tRNA guanylyl transferase (Cooley, L., Appel, B., and Söll, D. (1982) Proc. Natl. Acad. Sci. U. S. A. 79, 6475-6479). Here we show that the additional G of chloroplast tRNAHis species is gene-encoded and retained in the tRNA by a processing step analogous to the prokaryotic one. However, the structural requirements for recognition by the different RNase P activities are not the same.
Burkard
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U
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511.
Söll
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D
D000818:Animals; D001483:Base Sequence; D002736:Chloroplasts / Q000032:analysis*; D000458:Cyanobacteria / Q000201:enzymology; D004274:DNA, Recombinant; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D005056:Euglena gracilis / Q000235:genetics; D006150:Guanine Nucleotides; D006157:Guanosine Monophosphate; D008969:Molecular Sequence Data; D010430:Pentosyltransferases / Q000378:metabolism; D010944:Plants / Q000201:enzymology / Q000235:genetics; D012322:RNA Precursors / Q000235:genetics; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012354:RNA, Transfer, His / Q000235:genetics* / Q000378:metabolism; D043262:Ribonuclease P; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014675:Vegetables
D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D006150:Guanine Nucleotides; D012322:RNA Precursors; D012345:RNA, Transfer, Amino Acid-Specific; D012354:RNA, Transfer, His; D006157:Guanosine Monophosphate; D010430:Pentosyltransferases; C020837:queuine tRNA-ribosyltransferase; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli