Cloned extrachromosomal circular DNA copies of the human transposable element THE-1 are related predominantly to a single type of family member.
The 2300 base-pair transposon-like human element, THE-1, has been identified in the extrachromosomal circular DNA of the established human cell line HeLa as a relatively homogeneous population of covalently closed 1900 base-pair molecules. THE-1, which has been classified tentatively as a retroviral-like transposable element (a retrotransposon), is present in the extrachromosomal circular DNA of African green monkey (BSC-1) and human lymphoblastoid (Jurkat) cell lines. The 1900 base-pair extrachromosomal elements isolated and cloned from HeLa cells (1) appear to contain only THE-1-specific nucleotide sequences, (2) are circularized versions of the linear chromosomal sequence, and (3) are related predominantly to a single, or single type of, family member.
Misra
R
R
Department of Biochemistry, New York University School of Medicine, NY 10016.
Shih
A
A
Rush
M
M
Wong
E
E
Schmid
C W
CW
D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004270:DNA, Circular; D005111:Extrachromosomal Inheritance; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data
D004251:DNA Transposable Elements; D004270:DNA, Circular