Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis.
The complete type II restriction-modification system of Salmonella infantis was cloned in Escherichia coli as an R . Sau3AI fragment of 3,430 base pairs. The clone was shown to express the restriction endonuclease as well as the modification methylase. The nucleotide sequence of the above fragment showed two open reading frames of 461 and 230 codons in tail-to-tail orientation. These were shown to represent the modification methylase M . SinI and the restriction endonuclease R . SinI, respectively. The methylase M . SinI amino acid sequence revealed a considerable similarity to those of other deoxycytidylate methylases. In contrast, endonuclease R . SinI did not exhibit such a similarity to other restriction enzymes.
Karreman
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C
Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, The Netherlands.
de Waard
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A
D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015257:DNA-Cytosine Methylases; D015252:Deoxyribonucleases, Type II Site-Specific; D008780:Methyltransferases / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D012475:Salmonella / Q000235:genetics*; D012984:Software; D014158:Transcription, Genetic
D000596:Amino Acids; D003062:Codon; C056154:DNA modification methylase M.SinI; D015257:DNA-Cytosine Methylases; D008780:Methyltransferases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C038308:GGWCC-specific type II deoxyribonucleases