[Cytofluorometric and histopathological studies on non-cancerous liver lesions after lipiodol-transcatheter arterial embolization].
We have investigated the effects of preoperative Lipiodol-Transcatheter arterial embolization (Lp-TAE) on both the nuclear DNA content of noncancerous liver cells by DNA-cytofluorometry (using NIKON SPM-RF1-D), and the histopathological findings of liver lesions. Lp-TAE through the hepatic artery was performed on ten primary hepatocellular carcinoma patients using Gelfoam in combination with adriamycin (40 mg) or mitomycin C (20 mg), emulsified in Lipiodol (10 ml) and 60% Urografin (2 ml). And hepatic resection was carried out after one or two months later in each case. Histologically, primary hepatocellular carcinoma lesions showed various patterns of necrosis, while the non-cancerous liver cells did not show hepatocellular infarction in the liver cirrhosis. The results of the nuclear DNA content showed an increase in the fractions of poliploid cells in the non-cancerous lesions treated with Lp-TAE compared with that in the normal age-matched controls. But we found no remarkable differences in the ploidy patterns between Lp-TAE treated patients and untreated patients with liver cirrhosis. In conclusion, preoperative Lp-TAE does not induce any remarkable histological hepatocellular damage of non-cancerous lesion and does not affect nuclear DNA content of non-cancerous liver cells.
Shimotsuma
M
M
1st. Dept. of Surgery, Kyoto Prefectual University of Medicine.
Taniguchi
H
H
Sawai
K
K
Takahashi
T
T
Kagawa
K
K
Kamachi
M
M
Ashihara
T
T
Shimamura
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000628:therapy*; D004247:DNA / Q000032:analysis; D004317:Doxorubicin / Q000008:administration & dosage / Q000627:therapeutic use; D004621:Embolization, Therapeutic; D005260:Female; D005434:Flow Cytometry; D006499:Hepatic Artery; D006801:Humans; D007459:Iodized Oil / Q000008:administration & dosage / Q000627:therapeutic use*; D008099:Liver / Q000032:analysis / Q000473:pathology*; D008113:Liver Neoplasms / Q000235:genetics / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage / Q000627:therapeutic use; D011003:Ploidies
D008937:Mitomycins; D016685:Mitomycin; D007459:Iodized Oil; D004317:Doxorubicin; D004247:DNA