Effects of Escherichia coli lipopolysaccharide on the phosphoinositide metabolism and serotonin secretion in thrombin-activated platelets.
Lipopolysaccharide treatment of platelets in basal conditions does not produce any effect on serotonin secretion or on phosphatidic acid synthesis or arachidonic acid release. However, a brief exposure of platelets to endotoxin enhances the thrombin-stimulated activation of these parameters, whereas a more prolonged treatment with lipopolysaccharide impairs thrombin action. The results presented here also suggest that very short-term treatment of platelets with lipopolysaccharide activates the inositol 1,4,5-trisphosphate 3-kinase. The long-term effects of endotoxin could be mediated by protein kinase C activation.
Moscat
J
J
Department of Experimental Medicine, Hospital Provincial, Madrid, Spain.
Iglesias
S
S
Moreno
F
F
Herrero
C
C
Garcia-Barreno
P
P
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D004926:Escherichia coli; D066298:In Vitro Techniques; D010716:Phosphatidylinositols / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D012701:Serotonin / Q000378:metabolism*; D013552:Swine; D013556:Swine, Miniature; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors
D010716:Phosphatidylinositols; D011135:Polysaccharides, Bacterial; D012701:Serotonin; D013917:Thrombin