Use of viable-cell ELISA for detection of monoclonal antibodies recognizing tumor-associated antigens on bovine lymphosarcoma cells.
An ELISA, using viable bovine lymphosarcoma cells, was developed to detect tumor-associated antigens (TAA) expressed on lymphosarcoma cells from cows with enzootic bovine leukosis (EBL). Monoclonal antibodies (MAB) against TAA were used. Using viable-cell ELISA, MAB reacted with tumor cells from 9 cows with EBL, but not with peripheral blood lymphocytes from 10 clinically normal cows. Titers of MAB against tumor cells from 1 cow with EBL were 2,048 (direct immunofluorescence assay), 8,192 (flow cytometry), 2,048 (fixed-cell ELISA), and 16,384 (viable-cell ELISA). Viable-cell ELISA was the most sensitive method for detection of TAA. Reactivities of 7 MAB to tumor cells from cows with EBL were compared between viable-cell ELISA and a complement-dependent antibody cytotoxicity test. Of 5 MAB with no cytotoxic activity against tumor cells, 3 were reactive against tumor cells from the same cow, as determined by viable-cell ELISA, with titers ranging from 128 to 2,048.
Aida
Y
Y
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Onuma
M
M
Kasai
N
N
Izawa
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D001909:Leukemia Virus, Bovine; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor