title: The bovine leukemia virus p34 is a transactivator protein.
abstract: Recombinant Moloney murine retroviruses containing the BLV post-envelope long open reading frame were constructed and transfected into the psi 2 packaging cell line. They were shown to encode and to express a 34-kd protein able to transactivate the BLV long terminal repeat-directed gene expression in the respective transfected cells. These data demonstrate that the BLV X-LOR gene encodes a p34 transactivator product. Furthermore, the different cell lines produced infectious recombinant retroviruses capable of transferring X-LOR genes into recipient cells. The availability of the BLV transactivator protein should allow us to understand the role of the transactivator protein in BLV-induced leukemogenesis.
authors.affiliation: Faculty of Agronomy, Gembloux, France.
authors.forename: L
authors.initials: L
authors.lastname: Willems
authors.forename: A
authors.initials: A
authors.lastname: Gegonne
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Burny
authors.forename: R
authors.initials: R
authors.lastname: Kettmann
authors.forename: J
authors.initials: J
authors.lastname: Ghysdael
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D004247:DNA