Safety and efficacy of foot-and-mouth disease vaccines containing endonuclease-inactivated virions.
Inactivation of foot-and-mouth disease virus (FMDV) by means of virion-associated endonuclease was found to be suited to the production of safe and potent vaccines, which proved to be equal or better than those containing formaldehyde or ethyleneimine in guinea-pig potency tests. First order inactivation kinetics were regularly shown, with half life values which varied according to the different temperatures used. Inactivation brought about extensive degradation of FMDV RNA, while it did not adversely influence the integrity of critical viral epitopes on FMDV VP1.
Amadori
M
M
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
Barei
S
S
Melegari
M
M
Panina
G F
GF
D000818:Animals; D005537:Aphthovirus / Q000187:drug effects / Q000276:immunology*; D004720:Endonucleases / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D051379:Mice; D012367:RNA, Viral / Q000302:isolation & purification; D014613:Vaccines, Attenuated / Q000592:standards*
D012367:RNA, Viral; D014613:Vaccines, Attenuated; D004720:Endonucleases