[Synthesis and immunochemical properties of peptides corresponding to sequences 59-72 and 25-36 of human interleukin-2].
Synthesis of peptides corresponding to the 59-72 and 25-36 sequences of human IL-2 is reported. The former peptide, which had been shown to be immunogenic in the protein molecule, was prepared to obtain antipeptide antibodies for isolation and purification of the recombinant IL-2. We located the epitope at the C-terminus of this peptide. In accordance with the IL-2 secondary structure and hydrophilicity profile analysis, the 25-36 fragment was chosen as the potential epitope. The peptides were synthesized by conventional methods in solution, conjugated with a protein carrier, and polyclonal rabbit antisera were obtained. Antibodies against both peptides were shown to be specific to human IL-2 in ELISA. Besides, monoclonal antibodies to IL-2 recognized in ELISA the 59-72 peptide, suggesting the epitope located in this region to be main one in the protein molecule.
Onoprienko
L V
LV
Mikhaleva
I I
II
Lunev
V E
VE
Nesmeianov
V A
VA
Ivanov
V T
VT
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis* / Q000138:chemical synthesis / Q000276:immunology; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000138:chemical synthesis
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D010446:Peptide Fragments
Sintez i immunokhimicheskie svoĭstva peptidov posledovatel'nosti 59-72 i 25-36 interleĭkina-2 cheloveka.