Recombination by resolvase is inhibited by lac repressor simultaneously binding operators between res sites.
The Tn3 resolvase requires that the two recombination (res) sites be aligned as direct repeats on the same molecule for efficient recombination to occur. To test whether resolvase must contact the DNA between res sites as predicted by tracking models, we have determined the sensitivity of recombination to protein diffusion blockades. Recombination between two res sites is unaffected either by lac repressor or bacteriophage T7 RNA polymerase being bound between them. Yet recombination is inhibited by lac repressor if the res site is bounded by a lac operator on both sides. We demonstrate that lac repressor will bind to more than one DNA site under the conditions used to assay recombination. This result suggests that lac repressor can inhibit resolvase by forming a DNA loop that isolates a res site topologically. These results do not support a tracking model for resolvase but suggest that the structure and topology of the DNA substrate is important in the formation of a synapse between res sites.
Saldanha
R
R
Department of Biological Sciences, University of Notre Dame, IN 46556.
Flanagan
P
P
Fennewald
M
M
D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D009875:Operator Regions, Genetic / Q000187:drug effects*; D010957:Plasmids; D011995:Recombination, Genetic / Q000187:drug effects*; D012097:Repressor Proteins / Q000494:pharmacology*; D014157:Transcription Factors / Q000494:pharmacology*; D019895:Transposases
D012097:Repressor Proteins; D014157:Transcription Factors; D007544:Isopropyl Thiogalactoside; D004247:DNA; D009713:Nucleotidyltransferases; D019895:Transposases