RNA polymerase III promoter elements enhance transcription of RNA polymerase II genes.
Using transient expression assays in cultured human cells we have observed that RNA Polymerase III promoter sequences exert a positive cis-acting enhancer effect on RNA Polymerase II transcription. A DNA segment containing a copy of the Alu repeated element enhances transcription of the liver specific Haptoglobin related (Hpr) promoter in Hepatoma cell lines but not in HeLa cells. A tRNA(pro) gene acts as enhancer of the SV40 promoter both in Hepatoma and in HeLa cell lines. Transcription from the SV40 promoter is also enhanced by DNA segments containing only the box A or the box B of the tRNA(pro) promoter.
Oliviero
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S
European Molecular Biology Laboratory, Heidelberg, FRG.
Monaci
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P
D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005796:Genes; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D008113:Liver Neoplasms; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics*; D012320:RNA Polymerase III / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III