Recognition of simian virus 40 T antigen synthesized during viral lytic cycle in monkey kidney cells expressing mouse H-2Kb- and H-2Db-transfected genes by SV40-specific cytotoxic T lymphocytes leads to the abrogation of virus lytic cycle.
Simian virus 40 (SV40)-encoded tumor or T antigen localizes in the membranes in addition to the nucleus of SV40-infected permissive monkey cells and SV40-transformed nonpermissive cells. The surface T antigen in SV40-transformed mouse cells provides a target for the cytotoxic T lymphocytes (CTL) which recognize SV40 T antigen in association with murine K/D, class I H-2 antigens. In order to demonstrate that SV40 T antigen synthesized in SV40-infected permissive monkey kidney cells (TC-7) may also function as a target for CTL, cloned murine H-2Db and H-2Kb genes were expressed in TC-7 cells by DNA transfection and TC-7 cell lines expressing high levels of either H-2Kb or H-Db antigens were established after cell sorting. SV40-infected TC-7/H-2Kb and TC-7/H-2Db cells became susceptible to lysis by SV40-specific H-2b restricted CTL. The susceptibility of these transfected SV40-infected monkey cells to anti-SV40 bulk culture CTL and SV40-specific H-2Db- and H-2Db-restricted CTL clones depended upon the synthesis of SV40 T antigen and the expression of the appropriate H-2Kb or H-2Db restriction elements. Treatment of SV40-infected TC-7/H-2Db and TC-7/H-2Kb with CTL clones abrogated the virus lytic cycle indicating that CTL may play an important role in limiting papovavirus infection in the natural host.
Bates
M P
MP
Department of Microbiology, Pennsylvania State University, College of Medicine, Hershey 17033.
Jennings
S R
SR
Tanaka
Y
Y
Tevethia
M J
MJ
Tevethia
S S
SS
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000502:physiology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular; D013539:Simian virus 40 / Q000502:physiology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014779:Virus Replication
D000957:Antigens, Viral, Tumor; D006183:H-2 Antigens