Accuracy of intrachromosomal gene conversion in mouse cells.
Results of several recent studies suggest that homologous recombination and related processes in mammalian cells are highly mutagenic. We have examined the products of intrachromosomal gene conversion events that encompassed the last intron of the chicken thymidine kinase gene. Following plasmid rescue and DNA sequencing, we find no mutations associated with twenty conversion events representing 5380 total base pairs of which 2414 base pairs are intron sequence. Based on these studies we conclude that intrachromosomal gene conversion in mouse cells is not a highly mutagenic process but rather it operates with fidelity.
Stachelek
J L
JL
Department of Therapeutic Radiology, Yale School of Medicine, New Haven, CT 06510.
Liskay
R M
RM
D000818:Animals; D002645:Chickens; D002875:Chromosomes / Q000502:physiology*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D005785:Gene Conversion; D005796:Genes; D007739:L Cells / Q000201:enzymology; D051379:Mice; D011995:Recombination, Genetic; D013937:Thymidine Kinase / Q000235:genetics*
D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes