Fourier transform infrared assay of liposomal lipids.
Quantitating the lipid content in organic lipid solutions and extracted membrane preparations is described. Fourier transform infrared analysis of thin films using perdeuterated nonadecane as an internal standard permitted quantitation with greater than 95% accuracy.
Pidgeon
C
C
Purdue University School of Pharmacy, West Lafayette, Indiana 47907.
Apostol
G
G
Markovich
R
R
D000473:Alkanes; D003903:Deuterium; D005583:Fourier Analysis; D008081:Liposomes / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis*; D012015:Reference Standards; D012984:Software; D013055:Spectrophotometry, Infrared / Q000379:methods
D000473:Alkanes; D008081:Liposomes; D008563:Membrane Lipids; D003903:Deuterium; C061580:nonadecane