[Analysis of erythrocytic carrier molecules (glycosphingolipids) with ABO and Le specificity].
Membrane structures (stroma) were obtained from erythrocytes of various ABO and Le blood groups using a butanol/phosphate buffer extraction. The extracts were separated using thin layer chromatography and the ABO and Le antigens demonstrated directly on the chromatogram using the PAP-technique. Detection was carried out using either Ulex europaeus lectin or monoclonal antibodies. By this method the direct staining of the bands allowed a specific demonstration of the ABH and Leactive glycosphingolipids. A determination of the number of sugar residues is possible as well as the recognition of quantitative variants.
Hauser
R
R
Rand
S
S
Brinkmann
B
B
D000017:ABO Blood-Group System / Q000276:immunology*; D001788:Blood Grouping and Crossmatching; D002352:Carrier Proteins / Q000032:analysis; D004910:Erythrocyte Membrane / Q000032:analysis*; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*
D000017:ABO Blood-Group System; D002352:Carrier Proteins; D006028:Glycosphingolipids; D007983:Lewis Blood Group Antigens
Darstellung der erythrozytären Trägermoleküle (Glykosphingolipide) mit ABH- und Le-Spezifität.