Adenylate cyclase and G-proteins as a signal transfer system in the guinea pig inner ear.
In many eukaryotic cells G-proteins play a key role in signal transduction through outer cell membranes. To study this pathway in the auditory organ of mammals we examined tissue preparations from the stria vascularis and the organ of Corti from the guinea pig inner ear. The activity of adenylate cyclase was measured by stimulation at the site of the enzyme, the hormone receptors and the modulating G-proteins. In the organ of Corti we found a low enzyme activity in all cochlear turns. The stria vascularis, however, showed a constant high concentration of beta 2-adrenergic receptors and of stimulating G-proteins in all cochlear turns. In contrast, the activity of the enzyme increased from the apical to the basal turn. Adenylate cyclase could be stimulated or inhibited in a concentration-dependent manner by drugs selectively effecting the G-proteins. Our results suggest a structure of the adenylate cyclase complex in the inner ear similar to other organs. Pathophysiological correlations to hearing loss associated with pseudohypoparathyroidism are discussed.
Koch
T
T
Department of Otolaryngology, Medizinische Hochschule Hannover, Federal Republic of Germany.
Zenner
H P
HP
D000262:Adenylyl Cyclases / Q000032:analysis / Q000502:physiology*; D000818:Animals; D001306:Auditory Pathways / Q000201:enzymology*; D002772:Cholera Toxin / Q000493:pharmacokinetics; D003051:Cochlea / Q000201:enzymology*; D005576:Colforsin / Q000493:pharmacokinetics; D000242:Cyclic AMP / Q000032:analysis; D019204:GTP-Binding Proteins / Q000032:analysis / Q000502:physiology*; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000502:physiology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009925:Organ of Corti / Q000032:analysis / Q000201:enzymology*; D011943:Receptors, Adrenergic, beta / Q000032:analysis; D011953:Receptors, Cyclic AMP / Q000032:analysis; D013316:Stria Vascularis / Q000032:analysis / Q000201:enzymology*
D011943:Receptors, Adrenergic, beta; D011953:Receptors, Cyclic AMP; D005576:Colforsin; D006160:Guanosine Triphosphate; D002772:Cholera Toxin; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases