Microinjection of synthetic protein kinase inhibitor into single barnacle muscle fibers before and after cyclic AMP.
Single muscle fibers from the barnacle Balanus nubilus have been used as a preparation to see if a synthetic 20-residue PKI (5-24)-peptide is able to block or reverse the stimulatory response of the ouabain-insensitive Na efflux to injected cAMP. The results obtained show that this peptide behaves as a powerful inhibitor of the cAMP-mediated response and is also able to partially reverse the sustained stimulation of the Na efflux observed in ouabain-poisoned fibers following the injection of subunit A of cholera toxin.
Bittar
E E
EE
Department of Physiology, University of Wisconsin, Madison 53706.
Nwoga
J
J
D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D008845:Microinjections; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors; D012964:Sodium / Q000378:metabolism; D001468:Thoracica / Q000378:metabolism*
D010446:Peptide Fragments; D011480:Protease Inhibitors; D047428:Protein Kinase Inhibitors; C518603:protein kinase inhibitor peptide (5-24); D010042:Ouabain; D002772:Cholera Toxin; D012964:Sodium; D000242:Cyclic AMP