Expression of the C-terminal flanking peptide of human progastrin in human gastroduodenal mucosa, G-cell hyperplasia and islet cell tumours producing gastrin.
Three antisera to the C-terminally extended form of gastrin or the C-terminal flanking peptide of progastrin were used in an attempt to investigate the post-translational processing of progastrin at the cellular level by light and electron microscopical immunocytochemistry. In the normal human gastric antrum, the G-cell secretory granules were found to contain both gastrin and the C-terminal progastrin determinants (progastrin 87-93, 87-95 and 93-101). Immunostaining of serial sections at the light microscopical level revealed that duodenal gastrin-containing cells also express the C-terminal progastrin determinants, as well as gastrin-34. In foetal tissue, cells containing C-terminal gastrin and the C-flanking peptide of progastrin were first seen at 8 weeks of gestation, in the duodenum. They were not found in the stomach until the 11th week. In hyperplastic G-cells and in gastrin-producing tumour cells, the level of C-terminal peptide immunoreactivity was variable and often lower than that seen in normal antrum and only minimal immunoreactivity could be detected using electron immunocytochemistry. This was interpreted as representing altered post-translational processing of progastrin in modified G-cells.
Hara
M
M
Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Varndell
I M
IM
Bishop
A E
AE
Aitchison
M
M
Rode
J
J
Yamada
T
T
Green
D M
DM
Bloom
S R
SR
Polak
J M
JM
D007516:Adenoma, Islet Cell / Q000235:genetics* / Q000473:pathology; D004386:Duodenum / Q000166:cytology / Q000196:embryology; D005753:Gastric Mucosa / Q000032:analysis / Q000166:cytology / Q000473:pathology* / Q000648:ultrastructure; D005755:Gastrins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D006965:Hyperplasia; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology; D011498:Protein Precursors / Q000235:genetics*; D011706:Pyloric Antrum / Q000473:pathology*; D013270:Stomach / Q000166:cytology / Q000196:embryology
D005755:Gastrins; D007106:Immune Sera; D010446:Peptide Fragments; D011498:Protein Precursors; C013706:big gastrin