title: The antiviral effect of human interferon alpha is dependent on phosphoinositide-derived messengers.
abstract: Neomycin the putative blocker of membrane polyphosphoinositide hydrolysis, inhibited the antiviral activity of human interferon alpha, when tested on human quiescent fibroblasts challenged with vesicular stomatitis virus. The anti-interferon effect of neomycin could be correlated in terms of dose dependence for both neomycin (0.05-1 mM) and interferon (100-5,000 IU/ml). The results suggest that the antiviral activity of interferon alpha depends on diacylglycerol formation. Indeed, the synthetic diacylglycerol (50 microM) was as effective as 100 IU/ml interferon in inducing the antiviral state.
authors.affiliation: Stefan S. Nicolau Institute of Virology, Bucharest, Romania.
authors.forename: C
authors.initials: C
authors.lastname: Cernescu
authors.forename: S N
authors.initials: SN
authors.lastname: Constantinescu
authors.forename: F
authors.initials: F
authors.lastname: Baltă
authors.forename: L M
authors.initials: LM
authors.lastname: Popescu
mesh_terms: D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006868:Hydrolysis; D007370:Interferon Type I / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009355:Neomycin / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*
chemical_list: D007370:Interferon Type I; D010716:Phosphatidylinositols; D009355:Neomycin