Constitutive production of platelet-derived growth factor-like proteins by human prostate carcinoma cell lines.
Prostate carcinoma cell lines DU-145 and PC-3 express both platelet derived growth factor (PDGF)-1 and PDGF-2/sis genes. Concomitantly, these cells synthesize and secrete PDGF-like proteins, as judged by indirect immunofluorescence and by direct immunoprecipitation with specific PDGF antiserum. Conditioned media derived from DU-145 and PC-3 cells stimulated the incorporation of [3H]thymidine by 3T3 cells and competed with 125I-labeled PDGF for its binding to cell surface receptors of 3T3 cells. The biological activity was stable to heating at 100 degrees C for 10 min, sensitive to reducing agents, and neutralized by the IgG fraction of PDGF antiserum, properties similar to those of authentic PDGF. Both DU-145 and PC-3 cell lines appear to lack receptors for PDGF as indicated by their inability to mitogenically respond to PDGF and receptor binding of 125I-labeled PDGF. Production of PDGF-like proteins by human prostate carcinoma cells may play an important role in a paracrine mode in the organization of the extracellular matrix of the malignant tissue.
Sitaras
N M
NM
Division of Biological Sciences, Harvard School of Public Health, Boston, Massachusetts.
Sariban
E
E
Bravo
M
M
Pantazis
P
P
Antoniades
H N
HN
D002277:Carcinoma / Q000378:metabolism*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D014158:Transcription, Genetic
D009363:Neoplasm Proteins; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D012334:RNA, Neoplasm; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor