title: Adsorption of cytochrome c to phospholipid monolayers studied by reflection spectroscopy.
abstract: The uptake of cytochrome c by charged and neutral lipid monolayers was studied by using reflection spectroscopy. The method was shown to be a very sensitive and useful technique in studies of lipid-protein interactions. It was found that cytochrome c is preferentially bound to monolayers of negatively charged monolayers in the solid phase. Polarized light under oblique incidence was used to determine the average orientation of chromophores in cytochrome c bound to lipid monolayer. The transition moments of chromophore are oriented parallel to the monolayer plane.
authors.affiliation: Center for Marine Research, Rudjer Boskovic Institute, Zagreb, Croatia, Yugoslavia.
authors.forename: Z
authors.initials: Z
authors.lastname: Kozarac
authors.forename: A
authors.initials: A
authors.lastname: Dhathathreyan
authors.forename: D
authors.initials: D
authors.lastname: Möbius
mesh_terms: D003574:Cytochrome c Group / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D008081:Liposomes; D010743:Phospholipids; D011485:Protein Binding; D013053:Spectrophotometry
chemical_list: D003574:Cytochrome c Group; D008081:Liposomes; D010743:Phospholipids