title: Benzoate-dependent induction from the OP2 operator-promoter region of the TOL plasmid pWWO in the absence of known plasmid regulatory genes.
abstract: Expression of the lower catabolic pathway of the TOL plasmid pWWO requires an aromatic acid inducer and the product of the xylS regulatory gene. Pseudomonas putida cells transformed with a plasmid containing the operator-promoter region of the lower pathway (OP2 [or Pm]), upstream from the catechol 2,3-dioxygenase structural gene, showed enzyme induction in the absence of known TOL plasmid regulatory genes. Induction was not seen in transformed Escherichia coli cells or in a P. putida mutant lacking chromosomally encoded benzoate catabolic functions.
authors.affiliation: Environmental Research Laboratory, United States Environmental Protection Agency, Gulf Breeze, Florida.
authors.forename: S M
authors.initials: SM
authors.lastname: Cuskey
authors.forename: A B
authors.initials: AB
authors.lastname: Sprenkle
mesh_terms: D001565:Benzoates / Q000494:pharmacology; D050557:Catechol 2,3-Dioxygenase; D004262:DNA Restriction Enzymes; D049308:Dioxygenases; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009875:Operator Regions, Genetic; D010105:Oxygenases / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity; D014169:Transformation, Bacterial
chemical_list: D001565:Benzoates; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D004262:DNA Restriction Enzymes