Effect of cloned human interferon-alpha 2a on bovine parainfluenza-3 virus. Brief report.
Pretreatment of bovine turbinate (BTu) cells with cloned human interferon (IFN)-alpha 2a reduced the yield of infectious bovine parainfluenza-3 virus (PI-3 V). Intracellular synthesis of HN glycoprotein was reduced on postinfection day 1 (PID 1), but it recovered to normal levels subsequently. However, reduction of this protein persisted in the released virus through PID 2. Thin section electron microscopy demonstrated a drastically reduced release of mature virions and an accumulation of viral nucleocapsids inside the cytoplasm on PID 2. These results suggest that cloned human IFN-alpha 2a affects the glycoprotein synthesis, and morphogenesis of bovine PI-3 V, and thus inhibits the release of viral particles from treated cells.
Panigrahi
P
P
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park.
Mohanty
S B
SB
Maheshwari
R K
RK
Friedman
R M
RM
D000818:Animals; D002213:Capsid / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D006023:Glycoproteins / Q000096:biosynthesis; D007370:Interferon Type I / Q000494:pharmacology*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009024:Morphogenesis / Q000187:drug effects; D010224:Parainfluenza Virus 3, Human / Q000187:drug effects* / Q000254:growth & development; D012367:RNA, Viral / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D018108:Respirovirus / Q000187:drug effects*; D014758:Viral Core Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D006023:Glycoproteins; D007370:Interferon Type I; D012367:RNA, Viral; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins