Diminished IL-2-induced gamma-interferon production by unstimulated peripheral-blood lymphocytes in rheumatoid arthritis.
A diminished gamma-interferon (IFN-gamma) production by T cells from patients with rheumatoid arthritis (RA) in response to autologous stimulation coincides with a defective regulation of Epstein-Barr virus (EBV) transformation and is in part due to monocyte-produced interleukin-1 (IL-1) inhibitor and prostaglandins. Since IL-2 can act directly on unstimulated T-cells to induce IFN-gamma production we have now examined the effect of recombinant IL-2 (rIL-2) on purified resting T lymphocytes from RA patients. Treatment with rIL-2(25 U/ml) of lymphocytes from 15 controls led to an increased production of 72-h supernatant EBV-inhibitory activity (19 +/- 4% SE without; 48 +/- 7% with rIL-2), but had only minimal or no effect on gamma-interferon production by E-rosetting lymphocytes from 15 patients with active RA. This defect could not be corrected by adding indomethacin to RA cultures.
Hasler
F
F
Rheumatology Division, University Hospital Geneva, Switzerland.
Dayer
J M
JM
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood*; D001402:B-Lymphocytes / Q000502:physiology; D001773:Blood Cells / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000502:physiology; D007376:Interleukin-2 / Q000494:pharmacology / Q000502:physiology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification / Q000378:metabolism*; D008875:Middle Aged; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000378:metabolism
D007376:Interleukin-2; D011994:Recombinant Proteins; D007371:Interferon-gamma