Epidemiological studies of bovine spumavirus.
Bovine spumavirus (BSV) infection is shown to be endemic in some herds in north Queensland. The virus was readily isolated from leucocytes of the majority of mature cattle which were BSV antibody positive (BSV reactors) in the agar gel precipitin test (AGPT). Calves born to dams which were BSV reactors showed no BSV antibody or circulatory leucocyte-associated BSV (CLAB) at birth, but became BSV reactors following ingestion of colostrum, and maintained such evidence of passive immunity until 3-5 months of age. Experimental infection of dams with BSV at 5-7 months of gestation did not cause foetal infection. In groups of young animals at pasture, segregated by age and sex, no evidence of BSV infection occurred, following loss of passive immunity, until 18-24 months of age. At this time, occasional members of some groups (both male and female) became BSV reactors and showed CLAB. At approximately 24 months old, following mixing in groups with older cows, and single-sire mating, 34% of heifers became BSV reactors within 10 weeks. A herd survey indicated 85% of 2-3-year-old cows to be BSV reactors; thereafter, the percentage of reactors declined gradually with increasing age. Using BSV grown in cell culture, transmission of infection by throat spray was the most successful route, 7 of 7 (100%) of susceptible cattle becoming BSV reactors within 8 weeks of spraying. Using the intravenous route, only 2 of 5 (40%) susceptible cattle became BSV reactors, and swabbing of cell culture virus into the prepuce or vagina did not result in infection of 4 BSV-susceptible animals.(ABSTRACT TRUNCATED AT 250 WORDS)
Johnson
R H
RH
Graduate School of Tropical Veterinary Science, James Cook University, Townsville, Qld., Australia.
de la Rosa
J
J
Abher
I
I
Kertayadnya
I G
IG
Entwistle
K W
KW
Fordyce
G
G
Holroyd
R G
RG
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000209:etiology / Q000276:immunology / Q000635:transmission; D002478:Cells, Cultured; D003625:Data Collection; D005260:Female; D007112:Immunity, Maternally-Acquired; D008297:Male; D012190:Retroviridae / Q000302:isolation & purification*; D012192:Retroviridae Infections / Q000453:epidemiology / Q000209:etiology / Q000662:veterinary*; D016092:Spumavirus / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral