Gas chromatographic determination of mecarbam and its metabolites mecarboxon, diethoate, and diethoxon in cottonseeds.
A gas chromatographic method is described for determining residues of mecarbam and 3 of its metabolites, mecarboxon, diethoate, and diethoxon, in cottonseeds. For mecarbam analysis, following Soxhlet extraction with chloroform (after blending), the oily extract is partitioned with propylene carbonate and cleaned up on a silica gel column. Metabolites are extracted by the same method, followed by cleanup of mecarboxon on a silica gel column or diethoxon on an alumina column; cleanup of diethoate can be performed on either column. All 4 compounds are determined using a flame photometric detector equipped with a phosphorus filter. Average recoveries for cottonseed samples fortified with 0.03-1.0 ppm mecarbam ranged from 80 to 88%. Average recoveries were 81-88% for mecarboxon and 90-92% for diethoate (alumina column) and diethoxon from samples fortified with 0.05-1.0 ppm. Average recovery of diethoate from samples cleaned up on the silica gel column were 84-88% in the range of 0.05-0.2 ppm. Values obtained for mecarbam residues in field-treated samples are also presented.
Greenberg
R S
RS
Ministry of Agriculture, Plant Protection Department, Bet-Dagan, Israel.
D000537:Aluminum Oxide; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003368:Gossypium / Q000032:analysis* / Q000378:metabolism*; D007306:Insecticides / Q000032:analysis*; D063086:Organothiophosphates / Q000032:analysis* / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D058428:Silica Gel; D012822:Silicon Dioxide; D012997:Solvents
D007306:Insecticides; D063086:Organothiophosphates; D009946:Organothiophosphorus Compounds; D010573:Pesticide Residues; D012997:Solvents; D058428:Silica Gel; D012822:Silicon Dioxide; D000537:Aluminum Oxide; C054137:mecarbam