Muscarinic receptor-mediated intracellular Ca2+ mobilization in embryonic chick heart cells.
Activation of muscarinic receptors of heart cells elevates the intracellular Ca2+ concentration. The increase is considered to be due to influx of extracellular Ca2+. We show that intracellular Ca2+ mobilization is involved. Cell suspensions prepared from hearts of 6-day-old chick embryos were loaded with the fluorescent Ca2+ chelator chlortetracycline. Muscarinic stimulation induces a dose-dependent fluorescence decrease (ED50 = 2.6 x 10(-6) M) indicating intracellular Ca2+ mobilization.
Schmidt
H
H
Anatomisches Institut der Universität Tübingen, FRG.
Oettling
G
G
Drews
U
U
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002642:Chick Embryo; D002751:Chlortetracycline; D000242:Cyclic AMP / Q000096:biosynthesis; D005456:Fluorescent Dyes; D006321:Heart / Q000187:drug effects / Q000196:embryology; D009206:Myocardium / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000502:physiology*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D011976:Receptors, Muscarinic; D000242:Cyclic AMP; D000109:Acetylcholine; D002118:Calcium; D002751:Chlortetracycline