title: Plasmid and bacteriophage vectors for excision of intact inserts.
abstract: Plasmid (pPolyIII) and bacteriophage lambda (EMBL301) vectors are described in which sites for the rare-cutting enzymes SfiI and NotI (8-bp, recognition sequences) flank the polylinker cloning region. Intact DNA inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. General-purpose miniplasmid cloning vectors pPolyI and pPolyII are also described, and the utility of the bacteriophage lambda vector is demonstrated in the construction of a bovine genomic library.
authors.affiliation: LGME-CNRS, Strasbourg, France.
authors.forename: R
authors.initials: R
authors.lastname: Lathe
authors.forename: J L
authors.initials: JL
authors.lastname: Vilotte
authors.forename: A J
authors.initials: AJ
authors.lastname: Clark
mesh_terms: D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010957:Plasmids
chemical_list: D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides