The isolation and characterisation of cDNA and genomic clones for human lecithin: cholesterol acyltransferase.
The protein sequencing of tryptic peptides from purified human lecithin: cholesterol acyltransferase (LCAT) identified sufficient amino-acid sequence to construct a corresponding mixed oligonucleotide probe. This was used to screen an adult human cDNA liver library, from which incomplete cDNA clones were isolated. The DNA sequence of these clones allows the prediction of the entire amino-acid sequence of the mature LCAT enzyme. The mature protein consists of 416 amino acids and contains several marked stretches of hydrophobic residues and four potential glycosylation sites. The cDNA probe detects LCAT mRNA sequences approx. 1500 bases long in human liver, but not intestine, RNA. The cDNA probe was used to isolate LCAT genomic recombinants from a human genomic library. Southern blotting data, and restriction site mapping, suggest that there is a single human LCAT structural gene between 4.3 and 5.5 kb in size.
Tata
F
F
Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, University of London, U.K.
Chaves
M E
ME
Markham
A F
AF
Scrace
G D
GD
Waterfield
M D
MD
McIntyre
N
N
Williamson
R
R
Humphries
S E
SE
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood / Q000235:genetics*; D010957:Plasmids
D010446:Peptide Fragments; D004247:DNA; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D004262:DNA Restriction Enzymes