title: Association of herpes simplex virus regulatory protein ICP4 with sequences spanning the ICP4 gene transcription initiation site.
abstract: The HSV gene encoding ICP4 is negatively regulated and the HSV gene encoding thymidine kinase is positively regulated by ICP4 in vivo. We report that ICP4 is a component of a stable complex that contains protein and a sequence of approximately 28 nucleotides that span the ICP4 gene transcription initiation site. The association of ICP4 with DNA sequences between positions -103 and +32 relative to the ICP4 mRNA start site was demonstrated by DNA binding immunoassays. DNase footprinting revealed that nucleotides between positions -8 and +20 are protected by ICP4. In contrast, binding of ICP4 to sequences flanking the mRNA start site in the thymidine kinase gene was not observed. Models for ICP4-mediated positive or negative regulation of HSV gene transcription are discussed.
authors.affiliation: Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226.
authors.forename: S W
authors.initials: SW
authors.lastname: Faber
authors.forename: K W
authors.initials: KW
authors.lastname: Wilcox
mesh_terms: D001665:Binding Sites; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D017874:Immediate-Early Proteins; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D014157:Transcription Factors; D014764:Viral Proteins; C048925:herpes simplex virus, type 1 protein ICP4; D013937:Thymidine Kinase