Isolation and characterization of dengue viruses serotype 1 from an epidemic in northern Queensland, Australia.
Thirteen strains of dengue type 1 were isolated from the lymphocyte fractions of 69 acute phase blood samples collected at Thursday Island Hospital during 1981 and 1982. One further strain of type 1 was isolated from 7 blood samples despatched by air from Cairns Base Hospital during 1982. Four of these Australian isolates representing the beginning, middle, and end of the epidemic were examined by restriction enzyme mapping and were found to be identical for the nine restriction enzymes used. The maps differed from those derived from two Malaysian dengue type 1 strains isolated during the epidemic of 1981-82 in that country. This suggests reliance on serological typing to establish global circulation patterns of epidemic dengue is insufficient and that more specific methods such as genome mapping are useful.
Blok
J
J
Queensland Institute of Medical Research, Herston, Brisbane, Australia.
Kay
B H
BH
Hall
R A
RA
Gorman
B M
BM
D000293:Adolescent; D000328:Adult; D002648:Child; D004262:DNA Restriction Enzymes; D003715:Dengue / Q000453:epidemiology / Q000382:microbiology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007223:Infant; D008214:Lymphocytes / Q000382:microbiology; D008296:Malaysia; D008297:Male; D009693:Nucleic Acid Hybridization; D011793:Queensland; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D012703:Serotyping
D012367:RNA, Viral; D004262:DNA Restriction Enzymes