A rapid HPLC method to separate the triplet proteins of neurofilament.
In this article a fast HPLC technique to separate the individual neurofilament proteins is described. Highly pure fractions of the three neurofilament proteins can be obtained. As much as 50 mg of each neurofilament polypeptide can be separated from a crude neurofilament protein preparation in one step in less than 2 h. The short separation time is of importance in minimizing degradation, especially of the 150-kilodalton neurofilament polypeptide.
Karlsson
J E
JE
Institute of Neurobiology, University of Göteborg, Sweden.
Rosengren
L E
LE
Haglid
K G
KG
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D006882:Hydroxyapatites; D007118:Immunoassay; D007381:Intermediate Filament Proteins / Q000302:isolation & purification*; D016900:Neurofilament Proteins
D006882:Hydroxyapatites; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D017886:Durapatite