[Weakening of type I restriction in E. coli: the effect of 2-aminopurine and 5-bromouracil].
2-aminopurine (2-AP) and 5-bromouracil, strong mutagens of base analog type, were found to induce efficiently the alleviation of I type restriction in Escherichia coli. 2-AP induced restriction alleviation occurs in recA, lexA and mut- mutants, but no additional relief of restriction is registered in dam-bacteria in the presence of sublethal 2-AP concentrations. 2-AP specifically alleviates I type restriction in Escherichia coli (EcoA, EcoB, EcoD, and EcoK) and does not affect restriction systems of II (EcoRI) and III (EcoP1) types. We suggest that 2-AP-induced mismatches and other replication errors may be signals inducing restriction alleviation in Escherichia coli.
Belogurov
A A
AA
Efimova
E P
EP
Del'ver
E P
EP
Zavil'gel'skiĭ
G B
GB
D015075:2-Aminopurine / Q000494:pharmacology*; D000225:Adenine / Q000031:analogs & derivatives*; D001976:Bromouracil / Q000494:pharmacology*; D004260:DNA Repair; D004262:DNA Restriction Enzymes; D015253:Deoxyribonucleases, Type I Site-Specific; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D009154:Mutation
D015075:2-Aminopurine; D001976:Bromouracil; D004262:DNA Restriction Enzymes; C029602:endodeoxyribonuclease EcoK; D015253:Deoxyribonucleases, Type I Site-Specific; D000225:Adenine
Oslablenie restriktsii I tipa u E. coli: deĭstvie 2-aminopurina i 5-bromuratsila.