Effects of a phorbol ester on mouse eggs: dissociation of sperm receptor activity from acrosome reaction-inducing activity of the mouse zona pellucida protein, ZP3.
Biologically active phorbol esters or a diacylglycerol induce mouse eggs to modify the zona pellucida such that sperm receptor activity is retained but the ability of bound sperm to undergo a complete acrosome reaction is lost (Y. Endo, R.M. Schultz, and G.S. Kopf. 1987. Dev. Biol. 119, 199-209). We now show that purified ZP3 from 12-O-tetradecanoyl phorbol-13-acetate (TPA)-treated eggs possesses full sperm receptor activity but has lost its ability to induce a complete acrosome reaction. The modification of the acrosome reaction-inducing activity of ZP3 from these TPA-treated eggs differs from ZP3 isolated from two-cell embryos, which cannot initiate the acrosome reaction. These results demonstrate the dissociation of the two biological activities of ZP3 and may provide a system to assess the components of ZP3 involved in the acrosome reaction.
Endo
Y
Y
Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Mattei
P
P
Kopf
G S
GS
Schultz
R M
RM
D000177:Acrosome / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D004527:Egg Proteins; D005260:Female; D006023:Glycoproteins / Q000502:physiology*; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D010063:Ovum / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013094:Spermatozoa / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015044:Zona Pellucida / Q000187:drug effects / Q000502:physiology*; D000072216:Zona Pellucida Glycoproteins
D004527:Egg Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; C000606521:Zp3 protein, mouse; C035027:egg surface sperm receptor; D013755:Tetradecanoylphorbol Acetate