[Isolation of polymeric glycoprotein complexes of enveloped viruses using the Quill A glycoside and a study of their immunogenic activity].
Specific multimer complexes of glycoproteins of enveloped viruses were prepared by treatment of suspensions of purified concentrated virus with a non-ionic detergent MECK mixed with 0.2% glycoside Quil A. Mixed complexes of glycoproteins with glycoside Quil A were formed upon removal of the detergent from the mixture of solubilized glycoproteins and glycoside Quil A. According to the results of electron microscopy, the formed complexes differed morphologically from the conventional micelles of glycoproteins and presented spherical reticular structures 20-30 nm in diameter. The immunogenic potency of the complexes was much higher than that of virus particles and micelles of purified glycoproteins and was comparable to the immunogenic potency of glycoproteins mixed with complete Freund adjuvant. The protective activity of the complex of protein G of rabies virus with glycoside Quil A was higher than that of the subunit rabies vaccine adsorbed on aluminium hydroxide.
Berezin
V E
VE
Zaĭdes
V E
VE
Grigor'ev
V B
VB
Klimenko
S M
SM
Zhdanov
V M
VM
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D006027:Glycosides / Q000276:immunology / Q000494:pharmacology*; D007114:Immunization; D009980:Influenza A virus / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification; D011108:Polymers / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D062605:Quillaja Saponins; D011820:Rabies virus / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification; D012503:Saponins / Q000494:pharmacology*; D014197:Trees; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D006023:Glycoproteins; D006027:Glycosides; D008823:Micelles; D011108:Polymers; D062605:Quillaja Saponins; D012503:Saponins; D014759:Viral Envelope Proteins; C046386:Quil A
Poluchenie mul'timernykh kompleksov glikoproteidov obolochechnykh virusov s glikozidom Kvil A i izuchenie ikh immunogennoĭ aktivnosti.