Isolation and restriction endonuclease analysis of a mycoplasma plasmid.
A 1.7-kb plasmid was isolated from a large colony type strain of Mycoplasma mycoides subsp. mycoides. It was cloned into the Escherichia coli vector M13mp19, and DNA from the resultant recombinant was used to construct a restriction map of the plasmid. Because mycoplasmas show deviation from normal coding patterns, the availability of a mycoplasma plasmid could be useful in the development of a cloning vector for them.
Bergemann
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Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Finch
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LR
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D009174:Mycoplasma / Q000235:genetics*; D009710:Nucleotide Mapping; D010957:Plasmids
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes