Lactoferrin, myeloperoxidase, lysozyme and eosinophil cationic protein in exudate in delayed type hypersensitivity.
A skin window technique was used to study the morphology of leucocytes in upper dermis and exudate during nickel challenge in patients with contact allergy to nickel. Contact allergic patients and healthy volunteers tested with a skin widow without addition of nickel to the chamber medium served as controls. The morphology of the leucocytes in dermis was studied in biopsies taken 8, 24, or 48 h after skin window application, and in a parallel test the morphology of the exudate was examined by sequential collection of the chamber medium during a 48 h period. The infiltrate in dermis of contact allergic patients with nickel challenge in the chamber medium showed a time-dependent increase of mononuclear cells, eosinophils and basophils and a concomitant decrease of polymorphonuclear granulocytes, characteristic of a combined specific and unspecific inflammation. The morphology of the exudate in contact allergic patients exposed to nickel showed a dominance of polymorphonuclear granulocytes throughout the study period, while mononuclear cells, eosinophils and basophils were detected at a much lower quantity and with a considerable delay. Further, we studied the kinetics of the leucocyte granule proteins: lactoferrin, myeloperoxidase, lysozyme and eosinophil cationic protein in exudate fluid in a parallel test. A significant higher flux was found for all during the second day of allergen exposure compared to contact allergic patients without allergen challenge as well as normal volunteers. The increased protein fluxes were not accompanied by an increased flux of polymorphonuclear granulocytes in the exudate.(ABSTRACT TRUNCATED AT 250 WORDS)
Lerche
A
A
Dept. of Dermatology, Bispebjerg Hospital, Copenhagen, Denmark.
Bisgaard
H
H
Christensen
J D
JD
Venge
P
P
Dahl
R
R
Søndergaard
J
J
D000328:Adult; D000368:Aged; D001706:Biopsy; D001798:Blood Proteins / Q000032:analysis* / Q000493:pharmacokinetics; D002453:Cell Cycle / Q000187:drug effects; D003877:Dermatitis, Contact; D047091:Eosinophil Granule Proteins; D005122:Exudates and Transudates / Q000032:analysis* / Q000166:cytology; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000378:metabolism* / Q000503:physiopathology; D007781:Lactoferrin / Q000032:analysis* / Q000493:pharmacokinetics; D007782:Lactoglobulins / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism / Q000503:physiopathology; D009532:Nickel / Q000494:pharmacology; D009195:Peroxidase / Q000032:analysis* / Q000493:pharmacokinetics; D012260:Ribonucleases; D012867:Skin / Q000166:cytology; D012884:Skin Window Technique
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D007782:Lactoglobulins; D009532:Nickel; D009195:Peroxidase; D012260:Ribonucleases; D007781:Lactoferrin