Kinetic studies on the ATP- and ubiquitin-dependent proteolytic system of reticulocytes.
Half-maximal rate of the ATP- and ubiquitin-dependent proteolysis of rabbit reticulocytes, determined in a cell-free system, require 0.5 mg/ml of mitochondria-containing stroma (MCS) protein at a fixed ATP concentration or 100 microM ATP at a fixed protein concentration. 20 microM hemin caused 50% inhibition of degradation of MCS.
Dubiel
W
W
Institute of Biochemistry, Humboldt University Berlin, GDR.
Drung
I
I
Müller
M
M
Rapoport
S M
SM
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D006427:Hemin / Q000494:pharmacology; D007700:Kinetics; D010447:Peptide Hydrolases / Q000097:blood*; D010710:Phosphates / Q000097:blood; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*; D014452:Ubiquitins / Q000494:pharmacology*
D010710:Phosphates; D014452:Ubiquitins; D006427:Hemin; D000255:Adenosine Triphosphate; D010447:Peptide Hydrolases