title: Cloning, sequencing, and expression of the Lactobacillus casei thymidylate synthase gene.
abstract: The thymidylate synthase (TS) gene from Lactobacillus casei has been isolated, cloned, and sequenced. The coding sequence is 948 bp and predicts a primary structure, identical to that reported by protein sequencing methods (Maley et al., 1979b). The gene has been placed in several expression systems which complement TS-deficient Escherichia coli, and express the catalytically active enzyme at levels of 10-20% of the soluble protein of E. coli. The expressed TS has kinetic and structural properties consistent with its being identical to the authentic enzyme from L. casei.
authors.affiliation: Department of Biochemistry, University of California, San Francisco 94143.
authors.forename: K
authors.initials: K
authors.lastname: Pinter
authors.forename: V J
authors.initials: VJ
authors.lastname: Davisson
authors.forename: D V
authors.initials: DV
authors.lastname: Santi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D007780:Lacticaseibacillus casei / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010957:Plasmids; D013940:Thymidylate Synthase / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D013940:Thymidylate Synthase; D004262:DNA Restriction Enzymes