title: Characterization of canine adenovirus type 1 isolated from American black bears.
abstract: Viruses recovered from tissues taken at necropsy from American black bears were examined by use of immunofluorescence with polyclonal and monoclonal antibodies, virus neutralization with monoclonal antibodies, and restriction endonuclease analyses of the viral genomes. With these techniques, viruses were determined to be canine adenovirus type 1. Seronegative dogs that were inoculated with the virus had clinical signs typical of infectious canine hepatitis, suggesting that the virus, which was virulent for bears, was not a vaccinal strain, but a wild strain of canine adenovirus type 1.
authors.affiliation: National Animal Disease Center, US Department of Agriculture, Ames, IA 50010.
authors.forename: C A
authors.initials: CA
authors.lastname: Whetstone
authors.forename: H
authors.initials: H
authors.lastname: Draayer
authors.forename: J E
authors.initials: JE
authors.lastname: Collins
mesh_terms: D000257:Adenoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D006516:Adenoviruses, Canine / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000911:Antibodies, Monoclonal; D002335:Carnivora / Q000382:microbiology*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006522:Hepatitis, Infectious Canine / Q000382:microbiology*; D009500:Neutralization Tests; D001503:Ursidae / Q000382:microbiology*
chemical_list: D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D004262:DNA Restriction Enzymes