title: Regulation of casein kinase 2 by phosphorylation/dephosphorylation.
abstract: The effects of various polycation-stimulated (PCS) phosphatases and of the active catalytic subunit of the ATPMg-dependent (AMDc) protein phosphatase on the activity of casein kinase 2 (CK-2) were investigated by using the synthetic peptide substrate Ser-Glu-Glu-Glu-Glu-Glu, whose phosphorylated derivative is entirely insensitive to these protein phosphatases. Previous dephosphorylation of native CK-2 enhances its specific activity 2-3-fold. Such an effect, accounted for by an increase in Vmax, is more readily promoted by the PCS phosphatases than by the AMDc phosphatase. The phosphate incorporated by autophosphorylation could not be removed by the protein phosphatases, suggesting the involvement of phosphorylation site(s) other than the one(s) affected by intramolecular autophosphorylation. The activation of CK-2 by the phosphatase pretreatment is neutralized during the kinase assay; the mechanism of this phenomenon, which is highly dependent on the kinase concentration, is discussed.
authors.affiliation: Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit te Leuven, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Agostinis
authors.forename: J
authors.initials: J
authors.lastname: Goris
authors.forename: L A
authors.initials: LA
authors.lastname: Pinna
authors.forename: W
authors.initials: W
authors.lastname: Merlevede
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D047388:Casein Kinases; D002412:Cations / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D010455:Peptides / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000494:pharmacology*; D010766:Phosphorylation; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D047388:Casein Kinases; D002412:Cations; D010455:Peptides; D010749:Phosphoprotein Phosphatases; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D011494:Protein Kinases