title: Identification of mitochondrial and microsomal phosphatidylserine synthase in Saccharomyces cerevisiae as the gene product of the CHO1 structural gene.
abstract: In Saccharomyces cerevisiae, the membrane-associated enzyme phosphatidylserine synthase (EC 2.7.8.8) is present in the mitochondria and the endoplasmic reticulum. The enzyme from both membrane fractions reacted with antiserum raised against a hybrid protein expressed from a TRPE-CHO1 fusion gene in Escherichia coli and was absent in a cho1 null mutant, strongly suggesting that both the mitochondrial and microsomal forms of phosphatidylserine synthase are the products of the CHO1 gene. The highest degree of purification of enzymatically active protein was 380- and 420-fold from the mitochondrial and the microsomal compartments, respectively. In both cases, the enzymatically active and immunoreactive material comigrated with a protein band of 30,000 apparent molecular weight. In the absence of protease inhibitors during the preparation of membranes, the enzyme underwent degradation to an enzymatically active protein of 23,000 apparent molecular weight.
authors.affiliation: Institut für Biochemie, Technische Universität Graz, Austria.
authors.forename: S D
authors.initials: SD
authors.lastname: Kohlwein
authors.forename: K
authors.initials: K
authors.lastname: Kuchler
authors.forename: C
authors.initials: C
authors.lastname: Sperka-Gottlieb
authors.forename: S A
authors.initials: SA
authors.lastname: Henry
authors.forename: F
authors.initials: F
authors.lastname: Paltauf
mesh_terms: D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase / Q000032:analysis / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005800:Genes, Fungal; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D008970:Molecular Weight; D010770:Phosphotransferases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure
chemical_list: D010770:Phosphotransferases; D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase