Instability of rRNA operons in Bacillus subtilis.
Many laboratory strains of Bacillus subtilis contain 9 rather than 10 rRNA operons due to deletions occurring within the rrnJ-rrnW or rrnI-rrnH-rrnG gene cluster. These operons are members of two sets of closely spaced clusters located in the cysA-aroI region. Analysis of rescued DNA from integrants with insertions into rrnG and rrnH indicated that these tandemly arranged operons allowed frequent deletions of an rrn operon equivalent. These events may arise spontaneously by intrachromosomal recombination or by simultaneous double crossovers with a multimeric integrative plasmid.
Widom
R L
RL
Department of Biological Sciences, Hunter College of the City University of New York, New York 10021.
Jarvis
E D
ED
LaFauci
G
G
Rudner
R
R
D001412:Bacillus subtilis / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D009693:Nucleic Acid Hybridization; D009876:Operon; D010957:Plasmids; D011995:Recombination, Genetic; D012407:rRNA Operon
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; C041666:endodeoxyribonuclease BclI; D015252:Deoxyribonucleases, Type II Site-Specific