Chimaerism following allogeneic bone marrow transplantation: detection of residual host cells using the polymerase chain reaction.
Chimaerism was assessed in five recipients following sex mismatched allogeneic bone marrow transplantation. Techniques included karyotyping of bone marrow cells, dot blot DNA analysis of blood and bone marrow suspensions, and in vitro amplification of DNA by the polymerase chain reaction (PCR) using blood and bone marrow suspensions and stored bone marrow slides. Results of karyotypic analysis suggested complete chimaerism in four patients, while in one patient mixed chimaerism was detected. Mixed chimaerism was also detected, however, in a second patient using PCR and confirmed by dot blot analysis on all tissues examined. PCR is a sensitive tool for investigation of chimaerism following bone marrow transplantation. Since this technique does not require radioactivity, it is an attractive method for use in a clinical laboratory. This technique represents a further development in the use of DNA methodologies in the assessment of haematological disease.
Lawler
M
M
Department of Genetics, Trinity College Dublin, Ireland.
McCann
S R
SR
Conneally
E
E
Humphries
P
P
D015139:Blotting, Southern; D001853:Bone Marrow / Q000473:pathology; D016026:Bone Marrow Transplantation; D002678:Chimera; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D012730:Sex Chromosomes; D014184:Transplantation, Homologous