Periportal and perivenous hepatocytes respond equally to glycogenolytic agonists.
We have used the technique of short-term infusion with digitonin to obtain hepatocytes originating either from the periportal or the perivenous zone of the liver acinus [(1985) Biochem. J. 229, 221-226]. Total glycogen phosphorylase content and sensitivity to cyclic AMP-dependent and calcium-mediated glycogenolytic agonists were very similar for both cell sub-populations and did not differ from the values obtained for control cells. We conclude therefore that there is an apparent absence of metabolic zonation as far as receptor-mediated glycogenolysis and glycogenolytic potency is concerned.
Keppens
S
S
Afdeling Biochemie, Campus Gasthuisberg, Katholieke Universiteit Leuven, Belgium.
De Wulf
H
H
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology; D000242:Cyclic AMP / Q000502:physiology; D004072:Digitonin / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D010762:Phosphorylase a / Q000378:metabolism; D011168:Portal System; D051381:Rats; D011919:Rats, Inbred Strains; D014667:Vasopressins / Q000494:pharmacology; D014680:Veins
D014667:Vasopressins; D000804:Angiotensin II; D010656:Phenylephrine; D000255:Adenosine Triphosphate; D006003:Glycogen; D005934:Glucagon; D000242:Cyclic AMP; D005969:Glutamate Dehydrogenase; D010762:Phosphorylase a; D004072:Digitonin; D002118:Calcium