title: How to build an inexpensive cyclotherm instrument for automated polymerase chain reaction.
abstract: The Cyclotherm instrument is a functionally fully equivalent but inexpensive alternative to commercial instruments for automated polymerase chain reaction (PCR). It can be rebuilt under conditions of a biochemical laboratory for less than +1000. A Peltier element is used for heating and cooling of the reaction vials and the temperature and timing of the PCR cycles are controlled by a BASIC program in a SHARP PC 1600 low cost computer.
authors.affiliation: Mildred-Scheel-Labor für Krebszellforschung, Max-Planck-Institut, Martinsried, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Collasius
authors.forename: H
authors.initials: H
authors.lastname: Falk
authors.forename: C
authors.initials: C
authors.lastname: Ciesler
authors.forename: G
authors.initials: G
authors.lastname: Valet
mesh_terms: D003199:Computer Systems; D004259:DNA-Directed DNA Polymerase; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012984:Software
chemical_list: D004259:DNA-Directed DNA Polymerase