The hyg gene promoter from Streptomyces hygroscopicus: a novel form of Streptomyces promoters.
A 207 bp DNA fragment from the 5' region of the hyg gene of Streptomyces hygroscopicus was located preceding a DNA sequence encoding the mature form of human interferon alpha 2. This gene fusion, inserted in the Streptomyces vector pIJ702, expressed interferon activity in Streptomyces lividans indicating that the 207 bp sequence has promoter activity. The transcription initiation site was located. No significant homology with previously described Streptomyces promoters could be found. It appears therefore, it represents a novel class of Streptomyces promoters.
Pulido
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D
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Canto Blanco, Madrid, Spain.
Zalacaín
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Jiménez
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A
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004720:Endonucleases; D005798:Genes, Bacterial; D007370:Interferon Type I / Q000235:genetics; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
D007370:Interferon Type I; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases