title: A protoplast transformation system for Listeria sp.
abstract: We describe protoplast transformation of members of the genus Listeria with plasmid DNA. Shuttle vectors able to replicate in both Escherichia coli and Listeria have been constructed by fusing the E. coli plasmid pUC8 with the Bacillus subtilis plasmids pBD9 and pBD10.
authors.affiliation: Servicio de Microbiologia, Hospital Ramon y Cajal, Madrid, Spain.
authors.forename: M F
authors.initials: MF
authors.lastname: Vicente
authors.forename: F
authors.initials: F
authors.lastname: Baquero
authors.forename: J C
authors.initials: JC
authors.lastname: Perez-Diaz
mesh_terms: D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008087:Listeria / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D010957:Plasmids; D011523:Protoplasts / Q000502:physiology; D014169:Transformation, Bacterial
chemical_list: D004262:DNA Restriction Enzymes