Dibutyryl cyclic AMP decreases expression of ED-A fibronectin by canine chondrocytes.
The presence of Extra Domain A (ED-A) in fibronectins from cartilage and from the culture medium of chondrocytes cultured under various conditions was determined with monoclonal antibody IST-9. Less than 2% of the fibronectin extracted from cartilage was fibronectin which contained the ED-A sequence (ED-A fibronectin). Chondrocytes placed in primary culture began to synthesize increased amounts of ED-A fibronectin (6% to 36% of the total fibronectin). Expression of ED-A fibronectin was selectively reduced when the chondrocytes were cultured in the presence of 0.5 mM dibutyryl cyclic AMP.
Burton-Wurster
N
N
James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Leipold
H R
HR
Lust
G
G
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004285:Dogs; D000431:Ethanol / Q000494:pharmacology; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics*; D007700:Kinetics; D014212:Tretinoin / Q000494:pharmacology
D005353:Fibronectins; D000431:Ethanol; D014212:Tretinoin; D003994:Bucladesine