Immunoglobulin subclass antibodies to varicella-zoster virus.
Commercially available mouse monoclonal antibodies to human IgG subclass (IgG1 to IgG4) were applied to an enzyme-linked immunosorbent assay to measure IgG subclass-specific antibodies to varicella-zoster virus in children naturally infected with varicella-zoster virus and in varicella vaccine recipients. In children naturally infected with varicella-zoster virus, IgG 1 antibody was detected 2 weeks after onset of the disease in all cases, its activity increased at 1 month after onset, and almost equal antibody value was maintained 10 years after infection. This pattern of antibody response was similar to that of total IgG antibody to varicella-zoster virus after natural infection. On the other hand, low antibody activity was found in IgG2 only at 1 month of the disease. The highest antibody level of IgG3 was shown 2 weeks after onset of the disease; then, it gradually decreased, and no antibody activity was detected 10 years later. IgG4 antibody was first detected 1 month after onset and an almost equal level of antibody was shown 10 years after the disease. After inoculation of children with a live varicella vaccine, in contrast, IgG subclass antibody responses to vaccine recipients were almost equal to those after natural infection.
Asano
Y
Y
Department of Pediatrics, Fujita Gakuen Health University School of Medicine Aichi, Japan.
Hiroishi
Y
Y
Itakura
N
N
Hirose
S
S
Kajita
Y
Y
Nagai
T
T
Yazaki
T
T
Takahashi
M
M
D000914:Antibodies, Viral / Q000096:biosynthesis / Q000145:classification*; D002644:Chickenpox / Q000276:immunology; D019433:Chickenpox Vaccine; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000145:classification*; D014611:Vaccination; D014765:Viral Vaccines / Q000008:administration & dosage
D000914:Antibodies, Viral; D019433:Chickenpox Vaccine; D007074:Immunoglobulin G; D014765:Viral Vaccines