title: Bovine retinal pigment epithelium promotes proliferation of choroidal endothelium in vitro.
abstract: The invasion of Bruch's membrane and subsequently the subpigment epithelium and subretinal spaces by proliferating choroidal capillaries is a prominent feature of age-related macular degeneration. The ability to isolate and propagate pure cultures of bovine choroidal microvascular endothelium, choroidal pericytes, turbinate fibroblasts, and bovine retinal pigment epithelium allowed us to identify a growth-promoting activity produced by the retinal pigment epithelium that stimulated the proliferation of these cells in vitro. This mitogenic activity is present in both retinal pigment epithelium-conditioned medium and retinal pigment epithelium extract and enhances the mitogenic activity of retinal-derived extract. The activity is resistant to extremes of temperature and pH but partially sensitive to trypsin inactivation.
authors.affiliation: Retina Research Laboratory, Jules Stein Eye Institute, UCLA School of Medicine.
authors.forename: L S
authors.initials: LS
authors.lastname: Morse
authors.forename: J
authors.initials: J
authors.lastname: Terrell
authors.forename: Y
authors.initials: Y
authors.lastname: Sidikaro
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D002829:Choroid / Q000098:blood supply / Q000166:cytology*; D003470:Culture Media; D004730:Endothelium, Vascular / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D006133:Growth Substances / Q000032:analysis; D008833:Microcirculation; D010857:Pigment Epithelium of Eye / Q000032:analysis / Q000502:physiology*; D014420:Turbinates / Q000166:cytology
chemical_list: D003470:Culture Media; D006133:Growth Substances