Activation of recF-dependent recombination in Escherichia coli by bacteriophage lambda- and P22-encoded functions.
Escherichia coli strains bearing wild-type and mutant alleles of various recombination genes, as well as plasmids that express recombination-related genes of bacteriophages lambda and P22, were tested for their proficiency as recipients in Hfr-mediated conjugation. It was found that the homologous recombination systems of both phages could promote recombination in a recB recC mutant host. In addition, the Abc function of P22, but not the Gam function of lambda, was found to inhibit recombination in a wild-type host; however, both Abc and Gam inhibited recombination in a recF mutant host. These observations are interpreted as indicating that the recombination systems of both phages, as well as the RecBCD-modulating functions Abc and Gam, all activate the RecF recombination pathway of E. coli.
Poteete
A R
AR
Department of Molecular Genetics and Microbiology, University of Massachusetts, Worcester 01655.
Volkert
M R
MR
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005814:Genes, Viral; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic; D012483:Salmonella Phages / Q000235:genetics*
D001426:Bacterial Proteins