title: Heterogeneity in an isolated membrane protein. Has the 'authentic cytochrome oxidase' been identified?
abstract: The criteria of homogeneity or native state of a protein are prone to become ambiguous when applied to membrane proteins, such as cytochrome-c oxidase, which are purified by extraction with detergents. Properties of the purified material depend on the detergent used and on details of the purification protocol followed with any single batch of a preparation. We present arguments to show that the evidence presently available in published form does not justify the designation [(1987) J. Biol. Chem. 262, 3160-3164] of one type of preparation as being closer to the native state than others.
authors.affiliation: Department of Research, Alfred I. DuPont Institute, Wilmington, DE 19899.
authors.forename: C R
authors.initials: CR
authors.lastname: Hartzell
authors.forename: H
authors.initials: H
authors.lastname: Beinert
authors.forename: G T
authors.initials: GT
authors.lastname: Babcock
authors.forename: S I
authors.initials: SI
authors.lastname: Chan
authors.forename: G
authors.initials: G
authors.lastname: Palmer
authors.forename: R A
authors.initials: RA
authors.lastname: Scott
mesh_terms: D002462:Cell Membrane / Q000201:enzymology*; D003576:Electron Transport Complex IV / Q000302:isolation & purification* / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D003576:Electron Transport Complex IV