title: Adenovirus E1B 55-Mr polypeptide facilitates timely cytoplasmic accumulation of adeno-associated virus mRNAs.
abstract: Adenovirus provides helper functions that facilitate replication of adeno-associated virus (AAV). Both the adenovirus E1B 55-Mr and E4 34-Mr polypeptides are required for efficient and timely accumulation of AAV mRNA, proteins, and DNA. The E1B 55-Mr polypeptide is also required for rescue of the integrated AAV genome in Detroit 6-D5 cells in a normal time frame. All of these effects probably result from a single, primary delay in AAV mRNA accumulation. The AAV helper function provided by the E1B 55-Mr and E4 34-Mr polypeptides appears to closely parallel their normal role in the adenovirus replication cycle.
authors.affiliation: Department of Molecular Biology, Princeton University, New Jersey 08544.
authors.forename: R J
authors.initials: RJ
authors.lastname: Samulski
authors.forename: T
authors.initials: T
authors.lastname: Shenk
mesh_terms: D017456:Adenovirus Early Proteins; D002213:Capsid / Q000096:biosynthesis; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D000229:Dependovirus / Q000235:genetics*; D005786:Gene Expression Regulation; D006378:Helper Viruses / Q000502:physiology; D046911:Macromolecular Substances; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D014764:Viral Proteins / Q000502:physiology*; D014779:Virus Replication
chemical_list: D017456:Adenovirus Early Proteins; D004279:DNA, Viral; D046911:Macromolecular Substances; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins