Specificity of oxygen radical scavengers and assessment of free radical scavenger efficiency using luminol enhanced chemiluminescence.
The selective scavenging capacities of 19 important oxygen radical scavengers were determined by adding them individually to each of the four oxy radical standards, (superoxide, hydroxy, alkoxy and hydroperoxy, and singlet O2), calculating the percent chemiluminescence inhibited, and extrapolating O2 equivalents neutralized from baseline. The sensitivity (0.01 nm/ml) and selectivity of this method not only allows identification of individual oxygen free radical species but also quantitates the efficiency of free radical scavengers.
Rao
P S
PS
Division of Cardiothoracic Surgery, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
Luber
J M
JM
Milinowicz
J
J
Lalezari
P
P
Mueller
H S
HS
D001572:Benzofurans / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism*; D011724:Pyridazines / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
D001572:Benzofurans; D005609:Free Radicals; D011724:Pyridazines; D013481:Superoxides; C011238:1,3-diphenylisobenzofuran; D008165:Luminol; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D010100:Oxygen; D004121:Dimethyl Sulfoxide