Identification of two subtypes of serotype 4 human rotavirus by using VP7-specific neutralizing monoclonal antibodies.
Two distinct subtypes of human rotavirus serotype 4 were identified by using neutralizing monoclonal antibodies directed to the major outer capsid glycoprotein, VP7, of strains ST3 (subtype 4A) and VA70 (subtype 4B). Specimens containing serotype 4 rotavirus, obtained from different countries, were examined for subtyping by using solid-phase immune electron microscopy, enzyme-linked immunosorbent assay, and, for cell culture-adapted strains, neutralization assay. All 59 human rotavirus strains identified as serotype 4 by using animal antisera were classified into either subtype by monoclonal antibodies. This suggests that the antigenic difference between the two subtypes is a consequence of critical variations within the immunodominant serotype 4-specific neutralization site of rotavirus VP7. Subtype 4A (ST3-like) strains were predominant and were detected in stools from patients with gastroenteritis, as well as from healthy infants and young children.
Gerna
G
G
Institute of Infectious Diseases, University of Pavia, IRCCS Policlinco San Matteo, Italy.
Sarasini
A
A
di Matteo
A
A
Parea
M
M
Orsolini
P
P
Battaglia
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009500:Neutralization Tests; D012401:Rotavirus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal