Deletion and duplication variants around the long repeats of herpes simplex virus type 1 strain 17.
Three variants of herpes simplex virus type 1 strain 17 were isolated. One variant had a deletion of 2.5 X 10(6) Mr in IRL/UL and 0.8 X 10(6) Mr in TRL such that sequences were deleted from both long repeats. The deletion in UL removed the 20K and 22K open reading frames. The second variant had a deletion of 3.5 X 10(6) Mr in IRL/UL which again removed the 20K and 22K open reading frames. The third variant had a similar deletion in UL, but in this case the deleted sequences were replaced by sequences from the left end of UL, such that the long repeat was extended by 3 X 10(6) Mr and the overall genome size by 2 X 10(6) Mr. All three variants grew almost normally in vitro. The analysis of 11 isolates with extensive variation in the long repeats outside the 'a' sequence is also reported.
MacLean
A R
AR
MRC Virology Unit, University of Glasgow, U.K.
Brown
S M
SM
D005075:Biological Evolution; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D014644:Genetic Variation; D009154:Mutation; D012091:Repetitive Sequences, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics* / Q000254:growth & development; D014779:Virus Replication
D004279:DNA, Viral; D004262:DNA Restriction Enzymes