Mutants of the catabolite activator protein of Escherichia coli that are specifically deficient in the gene-activation function.
In the presence of cyclic AMP, the catabolite activator protein (CAP) of Escherichia coli binds DNA and stimulates transcription at a number of promoters. We have examined a model of CAP bound at the gal promoter and, using directed mutagenesis, have isolated CAP mutants that are analogous to the lambda repressor positive control (pc) mutants. These CAP mutants bind DNA but are defective in stimulating transcription at the gal P1 promoter. These mutants are also altered in positive control at the lac and malT promoters, where CAP binds to sites further upstream from the transcription start site.
Irwin
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N
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Ptashne
M
M
D001665:Binding Sites; D002373:Cyclic AMP Receptor Protein / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005690:Galactose; D005786:Gene Expression Regulation; D008961:Models, Structural; D009154:Mutation; D009690:Nucleic Acid Conformation; D009876:Operon; D011401:Promoter Regions, Genetic; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D002373:Cyclic AMP Receptor Protein; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases; D005690:Galactose