title: Separation of human plasma factor IX from HTLV-I or HIV by immunoaffinity chromatography using conformation-specific antibodies.
abstract: Immunoaffinity chromatography using conformation-specific antibodies yields pure factor IX from human plasma in a single rapid, facile purification step. We evaluated this technique to determine whether factor IX can be separated from human T cell leukemia virus-I (HTLV-I) and human immunodeficiency virus (HIV) in plasma supplemented with these viruses. Viral content was determined with an enzyme-linked immunosorbent (ELISA) assay sensitive to 50 ng viral protein. Both HTLV-I and HIV coeluted with unbound protein. Neither HTLV-I nor HIV was detected in purified factor IX. We conclude that, to the limits of detection, factor IX purified by this method is free of viral contamination.
authors.affiliation: Department of Medicine, New England Medical Center, Boston, MA.
authors.forename: S A
authors.initials: SA
authors.lastname: Limentani
authors.forename: B C
authors.initials: BC
authors.lastname: Furie
authors.forename: B J
authors.initials: BJ
authors.lastname: Poiesz
authors.forename: R
authors.initials: R
authors.lastname: Montagna
authors.forename: K
authors.initials: K
authors.lastname: Wells
authors.forename: B
authors.initials: B
authors.lastname: Furie
mesh_terms: D000906:Antibodies; D002846:Chromatography, Affinity / Q000379:methods; D017977:Deltaretrovirus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005164:Factor IX / Q000276:immunology / Q000302:isolation & purification*; D006678:HIV / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D011487:Protein Conformation
chemical_list: D000906:Antibodies; D005164:Factor IX