Expression of a possible constitutional "hot spot" in sperm chromosomes of a patient treated for Wilms' tumor.
Sperm chromosomes were studied in a man who was treated for Wilms' tumor with radiotherapy (RT) and chemotherapy (CT) 18 years ago. Human pronuclear sperm chromosomes were obtained after penetration of zona-free hamster eggs. Eighty-nine sperm chromosome complements were analyzed; 12.4% of them showed structural anomalies. This percentage was statistically different from the one found in our laboratory for controls (p less than 0.05). Five of eleven structurally abnormal metaphases had the same aberration: fission of chromosome #1 with the breakpoint at or near the centromere. Breaks and rearrangements of chromosome #1, often involving the centromere region, are among the most frequent anomalies found in Wilms' tumor cells.
Genescà
A
A
Department of Cellular Biology and Physiology, Facultat de Ciències, Universitat Autònoma de Barcelona, Spain.
Miró
R
R
Caballin
M R
MR
Benet
J
J
Navarro
J
J
Templado
C
C
Bonfill
X
X
Egozcue
J
J
D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects; D002871:Chromosome Banding; D002878:Chromosomes, Human, Pair 1; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000188:drug therapy / Q000532:radiotherapy / Q000628:therapy*; D008297:Male; D013094:Spermatozoa / Q000648:ultrastructure*; D009396:Wilms Tumor / Q000188:drug therapy / Q000532:radiotherapy / Q000628:therapy*
D000970:Antineoplastic Agents; D005819:Genetic Markers