[Intramolecular mobility of human major histocompatibility complex protein. A spin-label study].
Membranes of human splenocytes were hydrolyzed by papain and extracellular portions of class I and class II HLA antigen molecules were isolated by monoclonal antibodies fixed on Sepharose 4B. The isolated proteins were spin-labeled by TEMPO-dichlorotriazine and the values of rotational correlation times (tau) of labeled proteins were found using dependencies of ESR spectra parameters vs viscosity at constant temperature. The tau-values were equal to 8 ns for class I molecules and 14 ns for class II molecules. These values are 2-3 times lower than predicted for a rigid ellipsoid with mol wt. 50 kDa (about 20 ns). This fact suggests the existence of flexibility of HLA molecules which seems to be important for their biological activity. In this respect extracellular portions of HLA antigen molecules resemble flexible Fc fragments (tau = 12 ns) and differ from rigid Fab fragments (tau = 20 ns) of immunoglobulins G. The values of tau of spin-labeled proteins adsorbed from membrane hydrolysates on IgG-column was equal to 6.5 ns. The proteins adsorbed on lentil lectin column (after isolation of HLA proteins) have the tau-values equal to 9 ns.
Nezlin
R S
RS
Pankratova
E V
EV
Kul'guskin
V V
VV
Aruriunian
A E
AE
Timofeev
V P
VP
D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008285:Major Histocompatibility Complex; D013113:Spin Labels
D006680:HLA Antigens; D007074:Immunoglobulin G; D013113:Spin Labels
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