A solid-phase reverse immunosorbent test for the detection of enterovirus IgM.
IgM antibodies against enterovirus antigen were determined by solid-phase reverse immunosorbent test (SPRIST). The 145 sera studied were sampled from cases of enterovirus infections diagnosed by virus isolation and/or complement fixation. In 91 sera from enterovirus infections diagnosed by virus isolation 11/40, 16/28 and 9/23 were positive in SPRIST against ECHO 3 and/or Coxsackie B3 antigen during the first, during the second and third, and after 3 weeks of illness, respectively. The corresponding figures for 85 sera from enterovirus infections diagnosed by a greater than or equal to 4-fold rise in complement fixing (CF) antibody titre against antigen from ECHO 18 and/or Coxsackie B5 antigen were 10/39, 12/20 and 9/26. None out of 22 sera with rheumatoid factor reacted in SPRIST, but 1/92 and 1/154 sera from blood donors was positive in SPRIST with Coxsackie B3 and ECHO 3 antigen, respectively. IgM antibodies against ECHO 3 antigen as determined by SPRIST were found to be cross-reactive over a broad range of enterovirus types and positive results were recorded with sera from infections with Coxsackie A9, B3, B4, B5, ECHO 3, 9, 17, 18, 25 and 30. Heterotypic titres in SPRIST were of the same magnitude (up to 25,600) as recorded against the homotypic virus, 12,800 and 1,600, in two sera from one patient with an ECHO 3 infection. SPRIST was found to be a rapid convenient, cross-reactive and a cheap mu-capture assay for enteroviruses with more than 50% sensitivity during the second and third weeks after onset of illness.
Magnius
L O
LO
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
Saleh
L H
LH
Vikerfors
T
T
Norder
H
H
D003384:Coxsackievirus Infections / Q000175:diagnosis; D004457:Echovirus Infections / Q000175:diagnosis; D004770:Enterovirus / Q000276:immunology* / Q000302:isolation & purification; D029822:Enterovirus B, Human / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000175:diagnosis*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D007163:Immunosorbent Techniques; D013997:Time Factors
D007075:Immunoglobulin M