The rat prepro gastrin releasing peptide gene is transcribed from two initiation sites in the brain.
The gastrin-releasing peptide (GRP) is a gastrointestinal hormone, a neuropeptide, and a growth factor. To study systematically GRP gene expression in embryonic and in adult mammals, we cloned the rat prepro-GRP gene from brain cDNA libraries. Analysis of these cDNA clones along with organ-specific mRNA studies show that there are prepro-GRP transcripts initiating in the brain, but not in the duodenum, from a novel promoter. The latter is located at least four hundred base pairs upstream from a second promoter that is active in both duodenum and in brain. In contrast to human prepro-GRP, there is no alternative processing of the 3'-region of rat brain and duodenum prepro-GRP transcripts; hence, these mRNAs encode a single 147 amino acid prepro-GRP hormone that is homologous to the third isoform of the human prepro-GRP hormone.
Lebacq-Verheyden
A M
AM
National Cancer Institute, Naval Hospital, Bethesda, Maryland 20814.
Krystal
G
G
Sartor
O
O
Way
J
J
Battey
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D004386:Duodenum / Q000378:metabolism; D004720:Endonucleases; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; C047897:gastrin-releasing peptide precursor; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases