title: Electrophysiological analysis of beta-receptor stimulation in salamander proximal tubules.
abstract: Electrophysiological response to isoproterenol stimulation was studied in isolated perfused salamander proximal tubules. The addition of 10(-5) M isoproterenol to the bath superfusate depolarized both the cell membrane, and transepithelial potentials by 2.2 +/- 0.2 and 0.31 +/- 0.04 mV, respectively (P less than 0.01, n = 35) and significantly reduced the apical-to-basolateral membrane resistance ratio by 30% (P less than 0.01, n = 7) from a control value of 3.7 +/- 0.6. These responses were blocked by 10(-6) M propranolol but not mimicked by 10(-4) M adenosine 3',5'-cyclic monophosphate. Qualitatively similar effects were observed with 10(-3) and 10(-7) M isoproterenol. Further characterization of the 10(-5) M isoproterenol response revealed 1) a 50% reduction in the response following the removal of organic substrates from the luminal perfusate, 2) an absolute requirement for sodium, and 3) an absolute requirement for a functioning basolateral Na+-K+-ATPase. The data suggest that beta-receptor stimulation may increase sodium reabsorption by activating sodium cotransport systems.
authors.affiliation: Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: N S
authors.initials: NS
authors.lastname: Morgunov
mesh_terms: D000818:Animals; D004594:Electrophysiology; D007545:Isoproterenol / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D008433:Mathematics; D010042:Ouabain / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D012016:Reference Values; D014562:Caudata
chemical_list: D011943:Receptors, Adrenergic, beta; D010042:Ouabain; D011433:Propranolol; D007545:Isoproterenol