Temporal association of calcium mobilization, inositol trisphosphate generation, and superoxide anion release by human neutrophils activated by serum opsonized and nonopsonized particulate stimuli.
We have investigated the involvement of phospholipase C mediated polyphosphoinositide turnover in activation of polymorphonuclear leukocytes by particulate stimuli. Results showed that stimulation of leukocytes with serum opsonized zymosan (ingestible particle), serum opsonized Candida albicans hyphae (noningestible particle), or nonopsonized hyphae was followed by a transient rise in cellular inositol phosphates as has been described for neutrophil activation via the formyl peptide receptor. The kinetics of inositol trisphosphate generation paralleled both the time course of changes in cytosolic calcium concentration and the onset of superoxide anion generation, suggesting that these may be related events.
Meshulam
T
T
Evans Memorial Department of Clinical Research, Boston University Medical Center, Massachusetts 02118.
Diamond
R D
RD
Lyman
C A
CA
Wysong
D R
DR
Melnick
D A
DA
D002118:Calcium / Q000097:blood*; D002176:Candida albicans; D003600:Cytosol / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000097:blood*; D007700:Kinetics; D009504:Neutrophils / Q000378:metabolism*; D013403:Sugar Phosphates / Q000097:blood*; D013481:Superoxides / Q000097:blood*; D015054:Zymosan
D007295:Inositol Phosphates; D013403:Sugar Phosphates; D013481:Superoxides; D015544:Inositol 1,4,5-Trisphosphate; D015054:Zymosan; D002118:Calcium