title: Separation of simian virus 40 large-T-antigen-transforming and origin-binding functions from the ability to block differentiation.
abstract: Wild-type simian virus 40 large T antigen is very effective at blocking adipocyte differentiation in 3T3-F442A cells as assayed by triglyceride accumulation, induction of glycerophosphate dehydrogenase activity, and expression of mRNAs for glycerophosphate dehydrogenase, the adipocyte serine protease adipsin, and the putative lipid-binding protein adipocyte P2. Point mutants defective for either origin-specific DNA binding or transformation blocked differentiation as completely as wild type.
authors.affiliation: Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
authors.forename: V
authors.initials: V
authors.lastname: Cherington
authors.forename: M
authors.initials: M
authors.lastname: Brown
authors.forename: E
authors.initials: E
authors.lastname: Paucha
authors.forename: J
authors.initials: J
authors.lastname: St Louis
authors.forename: B M
authors.initials: BM
authors.lastname: Spiegelman
authors.forename: T M
authors.initials: TM
authors.lastname: Roberts
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000276:immunology*; D002454:Cell Differentiation; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D007118:Immunoassay; D009154:Mutation; D009857:Oncogenes; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology*
chemical_list: D000952:Antigens, Polyomavirus Transforming