Immunohistochemical study of angiotensin-converting enzyme in human tissues using monoclonal antibodies.
The localization of angiotensin-converting enzyme (ACE) in human tissues has been studied by the PAP-method with the use of monoclonal antibody 9 B9 against human lung ACE. The enzyme was detected on the surface of endothelial cells in lung, myocardium, liver, intestine and testis as well as in the epithelial cells of the kidney proximal tubules and intestine. The monoclonal antibody 9 B9 did not react with ACE in the epithelial cells of the testis seminiferous tubules. These data suggest that the antibody 9 B9 recognizes epitope which is shared by the ACE molecule of endothelial cells and renal and intestinal epithelial cells but is not present in testicular ACE, or is not accessible there to the antibody.
Danilov
S M
SM
Institute of Experimental Cardiology, USSR Cardiology Research Center, Moscow.
Faerman
A I
AI
Printseva OYu
Martynov
A V
AV
Sakharov IYu
Trakht
I N
IN
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D006801:Humans; D007124:Immunoenzyme Techniques; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000201:enzymology; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D013737:Testis / Q000201:enzymology
D000911:Antibodies, Monoclonal; D007703:Peptidyl-Dipeptidase A