title: Cloning and sequencing of a gene encoding an aminoglycoside 6'-N-acetyltransferase from an R factor of Citrobacter diversus.
abstract: The aacA1 gene, which encodes a 6'-N-acetyltransferase [AAC(6')-I] mediating resistance to kanamycin, tobramycin, and amikacin, was cloned from the Citrobacter diversus R plasmid pBWH100 into the Escherichia coli vector pBR322. The complete nucleotide sequence of the gene and flanking regions was determined. A protein of approximately 21 kilodaltons was identified when the chimeric plasmid encoding the aacA1 gene was introduced into E. coli maxicells. This value is consistent with the size predicted for a protein translated from the open reading frame of the gene.
authors.affiliation: Seattle Veterans Administration Medical Center, Washington 98108.
authors.forename: F C
authors.initials: FC
authors.lastname: Tenover
authors.forename: D
authors.initials: D
authors.lastname: Filpula
authors.forename: K L
authors.initials: KL
authors.lastname: Phillips
authors.forename: J J
authors.initials: JJ
authors.lastname: Plorde
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002954:Citrobacter / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011815:R Factors
chemical_list: D004269:DNA, Bacterial; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; D004262:DNA Restriction Enzymes