A mimic of superoxide dismutase activity based upon desferrioxamine B and manganese(IV).
MnO2 reacted with desferrioxamine B yielding a green, water-soluble complex, with absorption maxima at 315 and 635 nm whose extinction coefficients were 925 and 60 M-1 cm-1, respectively. Increasing the proportion of ligand to metal increased both color yield and ability to scavenge O2-, with maximal color yield and activity being achieved at a 1:1 ratio. The complex catalyzed the dismutation of O2- and 1 microM was equivalent to 1 unit of superoxide dismutase activity in the xanthine oxidase-cytochrome c assay. The complex thus exhibited approximately 0.1% as much activity as did the manganese-containing superoxide dismutase, on the basis of manganese content. The activity of the complex was not suppressed by bovine serum albumin or by the soluble proteins extracted from Lactobacillus plantarum. In contrast, the activities of Cu(II) complexes of salicylate or Gly-His-Lys were suppressed by these proteins.
Darr
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Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
Zarilla
K A
KA
Fridovich
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I
D003676:Deferoxamine / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D008345:Manganese / Q000378:metabolism*; D017895:Manganese Compounds; D010087:Oxides; D011485:Protein Binding; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides
D008024:Ligands; D017895:Manganese Compounds; D010087:Oxides; D013481:Superoxides; D008345:Manganese; D013482:Superoxide Dismutase; D003676:Deferoxamine; C016552:manganese dioxide