Methods for recovering poliovirus and rotavirus from oysters.
Polioviruses and rotaviruses are potential indicators of sewage pollution of water and shellfish. Several methods for detecting these viruses in oysters were assessed. Elution-precipitation involving Catfloc for clarification and skim milk for subsequent flocculation resulted in the recovery of an average of 79% of poliovirus type 1 and 37% of rotavirus SA-11 from oyster homogenates inoculated with low numbers of these viruses. Adsorption-elution-precipitation did not improve the recovery of poliovirus and was detrimental to the recovery of rotavirus. Ultrafiltration or ultracentrifugation resulted in improved recovery of rotavirus but also in higher toxicity of oyster extracts to cell cultures. We recommend the use of the described elution-precipitation method for detecting viral pollutants in sample of oysters.
Speirs
J I
JI
Health Protection Branch, Health and Welfare Canada, Ottawa, Ontario.
Pontefract
R D
RD
Harwig
J
J
D000818:Animals; D002460:Cell Line; D005427:Flocculation; D006863:Hydrogen-Ion Concentration; D010125:Ostreidae / Q000382:microbiology*; D017955:Poliovirus / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification*; D014461:Ultracentrifugation; D014462:Ultrafiltration; D014773:Virology / Q000379:methods