Preliminary X-ray diffraction analysis of HhaII endonuclease-DNA cocrystals.
HhaII restriction endonuclease purified from an overproducing recombinant E. coli clone has been cocrystallized with a heptanucleotide duplex, d-GGAGTCC:GGACTCC. The cocrystals are monoclonic and belong to the space group C2. The unit cell dimensions are a = 199.0 +/- 1.0 A, b = 100.0 +/- 0.5 A, c = 80.3 +/- 0.4 A, and beta = 101.0 +/- 1.0 degrees. There appear to be two dimers per asymmetric unit and the crystals diffract to 4-A resolution.
Chandrasegaran
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Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Smith
H O
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Amzel
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Ysern
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X
D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D004247:DNA; D004262:DNA Restriction Enzymes / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics; D009838:Oligodeoxyribonucleotides; D014961:X-Ray Diffraction
D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases