title: Changes of membrane fluidity and Na+,K+-ATPase activity during cellular differentiation in the guinea pig epidermis.
abstract: The dynamic properties of plasma membrane in epidermal cells were determined by means of electron spin resonance using two kinds of doxyl stearic acid spin labeling agents: 5-DSA and 12-DSA. 5-DSA and 12-DSA are stearic acid analogues with a nitroxide radical ring at the 5th and 12th carbon positions, and these motions reflect molecular motion of lipid bilayer surrounding the hydrophilic region and the hydrophobic region, respectively. Guinea pig epidermal cells were separated into three regions of keratinocytes by Percoll density gradient centrifugation; the upper, middle, and lower epidermal cells. The order parameter S values for 5-DSA and 12-DSA incorporated into the isolated keratinocytes increased, suggesting a decrease in the plasma membrane fluidity, as cells approached the upper epidermal cell layer. The Na+,K+-ATPase activity as a plasma membrane-bound enzyme was determined in each epidermal cell region, and was found to decrease gradually as the cells approached the upper layer. Accordingly, the differentiation of epidermal cells in the keratinization process was found to be associated with a decrease in plasma membrane fluidity and with a decline of Na+,K+-ATPase activity.
authors.affiliation: Department of Medical Biochemistry, Kurume University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Hidaka
authors.forename: R
authors.initials: R
authors.lastname: Ogura
authors.forename: M
authors.initials: M
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D006168:Guinea Pigs; D008560:Membrane Fluidity; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013113:Spin Labels
chemical_list: D003497:Cyclic N-Oxides; D013113:Spin Labels; C037404:12-doxylstearic acid; C005072:5-doxylstearic acid; D000254:Sodium-Potassium-Exchanging ATPase