Retroviral gag gene amber codon suppression is caused by an intrinsic cis-acting component of the viral mRNA.
In some type C retroviruses, translation of the pol gene appears to require translational suppression of the proximal gag amber codon. To identify the region of the viral nucleic acid responsible for synthesis of the pol gene products, a 300-base-pair DNA fragment containing the stop codon from a type C murine virus (AK virus) was inserted into the Escherichia coli lacZ gene such that the translational reading frame was maintained. Introduction of the resulting fusion gene into cells resulted in the suppression of the viral stop codon. As measured by beta-galactosidase production, suppression occurred at a frequency of approximately 10%. Suppression could occur in at least several vertebrate cell types and was not augmented by virus replication or the expression of viral gene products. This indicates that gag amber codon suppression does not require augmented levels of suppressor tRNA species.
Panganiban
A T
AT
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase; D003062:Codon / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013489:Suppression, Genetic; D014162:Transfection; D014779:Virus Replication
D003062:Codon; D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases