Nucleotide sequence of the androgen-dependent arginine esterase mRNA of canine prostate.
The nucleotide sequence of canine prostate arginine esterase mRNA was determined using a 400 bp cDNA clone and primer-extended cDNA transcripts for the 5'-coding and noncoding regions. The mRNA contains 864 nucleotides encoding a protein of 236 amino acids preceded by 24 amino acids which constitutes both the signal and the zymogen peptides. The sequence indicates the presence of one potential glycosylation site. A high degree of homology was found between the canine enzyme and other members of the kallikrein family including human prostate specific antigen. The protein appears to be specified by a single gene.
Chapdelaine
P
P
Laboratory of Hormonal Bioregulation, Laval University Hospital Centre, Sainte-Foy, Québec, Canada.
Ho-Kim
M A
MA
Tremblay
R R
RR
Dubé
J Y
JY
D000595:Amino Acid Sequence; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004285:Dogs; D007610:Kallikreins / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011467:Prostate / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D000728:Androgens; D012333:RNA, Messenger; D004247:DNA; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D004262:DNA Restriction Enzymes; D007610:Kallikreins