Induction of the RI cAMP-binding protein in the "neurite-minus" N1A-103 mouse neuroblastoma cell line.
We examined the effects of agents/conditions that raise intracellular cAMP concentration in the induction of the 47,000-dalton RI cAMP-binding protein in the "neurite-minus" N1A-103 mouse neuroblastoma cells. The amount of RI in cell extracts was quantitated by photoaffinity labeling with 8-N3-[32P]cAMP and by ELISA and Western blot. Our results showed that treatment of the N1A-103 neuroblastoma cells with 20 microM forskolin or 1 mM dibutyryl cAMP, or reducing the serum concentration in the culture medium from 10% to 1% caused a 3-4 fold increase in the amount of RJ. Our results suggest that the induction of RJ can occur independent of morphological differentiation in the mouse neuroblastoma cells.
Liu
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AY
Department of Biological Sciences, Rutgers, University, Piscataway, N.J. 08855.
Kamalakannan
V
V
Lin
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D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D001386:Azides / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism; D002373:Cyclic AMP Receptor Protein; D003600:Cytosol / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*
D000345:Affinity Labels; D001386:Azides; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; C017042:8-azidoadenosine-3',5'-monophosphate; D000242:Cyclic AMP