Suppression of renin-angiotensin system in the heart of spontaneously hypertensive rats.
Renin-like enzyme and angiotensin converting enzyme (ACE) were identified and their specific activities measured in cardiac tissues of spontaneously hypertensive rats (SHR) and their Wistar-Kyoto (WKY) normotensive controls. In addition, the enzyme activities were determined following administration of hypotensive drugs. The pH optima of cardiac renin-like enzymes were identical with those in vascular walls, the specific activity being higher in the heart. Cardiac ACE revealed similarities with the venous wall enzyme. The highest specific cardiac renin-like activity was found in the septum and that of ACE in atria/auricles. Both enzyme values were lower in the hearts of SHR than in those of normotensive controls. Following nifedipine treatment, specific renin-like activities increased in all cardiac structures studied (P less than 0.01); with nitrendipine and muzolimine less pronounced elevations were obtained. Administration of these three hypotensive drugs resulted in a stimulation of ACE in all the cardiac structures except in atria/auricles, where their activities were lowered.
Rosenthal
J
J
Ulm University Medical Centre, Federal Republic of Germany.
von Lutterotti
N
N
Thurnreiter
M
M
Gomba
S
S
Rothemund
J
J
Reiter
W
W
Kazda
S
S
Garthoff
B
B
Jacob
I
I
Dahlheim
H
H
D000818:Animals; D000959:Antihypertensive Agents / Q000627:therapeutic use; D002853:Chromatography, Liquid; D006321:Heart / Q000187:drug effects; D006973:Hypertension / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009156:Muzolimine / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D009543:Nifedipine / Q000494:pharmacology; D009568:Nitrendipine / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012084:Renin-Angiotensin System
D000959:Antihypertensive Agents; D009156:Muzolimine; D009568:Nitrendipine; D007703:Peptidyl-Dipeptidase A; D009543:Nifedipine