title: Monoclonal antibodies specific for high molecular weight form of human epidermal growth factor.
abstract: Hybridomas that secrete monoclonal antibodies specific for the high molecular weight (HMW) form of human epidermal growth factor (hEGF) were established by fusing spleen cells obtained from mice immunized with purified urinary HMW-hEGF with myeloma P3 x 63Ag8.653. The resulting monoclonal antibodies were characterized basically into two groups. One group recognized both EGF and HMW-hEGF, while the other recognized HMW-hEGF specifically on radio immunoprecipitation. Surprisingly, the majority of the isolates was positive by western blotting. Utilizing these monoclonal antibodies for affinity chromatography, we purified HMW-hEGF successfully from urine. These antibodies may be an extraordinarily powerful tool for histological study related to both forms of EGF.
authors.affiliation: Biotechnology Research Laboratories, JCR Pharmaceuticals Co., Ltd., Kobe, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Koga
authors.forename: S
authors.initials: S
authors.lastname: Nishimuro
authors.forename: H
authors.initials: H
authors.lastname: Shirono
authors.forename: C
authors.initials: C
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: A
authors.initials: A
authors.lastname: Matsuo
authors.forename: M
authors.initials: M
authors.lastname: Kurobe
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004815:Epidermal Growth Factor / Q000276:immunology / Q000652:urine*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011233:Precipitin Tests / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D004815:Epidermal Growth Factor