Construction of cosmid genomic libraries for the normal and myopathic Syrian hamsters.
Cosmid genomic libraries from both normal and myopathic Syrian hamsters have been constructed. MboI was used to generate 35- to 50-kilobase DNA fragments which were isolated from a 5-25% NaCl gradient. The 35- to 50-kilobase DNA fragments were ligated to the cosmid vector pCV108 and packaged into Escherichia coli DK1. Approximately 3 X 10(5) - 4 X 10(5) clones were obtained per microgram of ligated DNA. Thirteen clones have been isolated from 2 X 10(5) colonies using a cardiac myosin heavy chain clone as a probe. Restriction maps of two of these clones are presented here.
McCully
J D
JD
Department of Clinical Biochemistry, Banting Institute, University of Toronto, Ont., Canada.
Jandreski
M A
MA
Liew
J
J
Sole
M J
MJ
Liew
C C
CC
D000818:Animals; D009202:Cardiomyopathies / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae / Q000235:genetics*; D004262:DNA Restriction Enzymes; D008647:Mesocricetus / Q000235:genetics*; D008970:Molecular Weight; D009218:Myosins / Q000235:genetics*
D004262:DNA Restriction Enzymes; D009218:Myosins