title: Characterization of the internal initiation of translation on the vesicular stomatitis virus phosphoprotein mRNA.
abstract: Internal initiation of translation on the vesicular stomatitis virus (VSV) phosphoprotein (P) mRNA leads to the synthesis of a second protein [Herman, R. C. (1986) J. Virol. 58, 797-804]. Characterization of this phenomenon shows that initiation at the 5'-proximal and internal AUG codons has different optima for mono- and divalent cations in the reticulocyte lysate. Whereas 5' initiation is stimulated by increasing concentration of K+ over the endogenous level, internal initiation is inhibited. Internal initiation is much less sensitive to the effects of the cap analogue 7mGpppG in both the reticulocyte lysate and the wheat-germ extract under conditions that reduce 5'-proximal initiation to only about 4-5% of the control level. These results imply that 5'-proximal and internal initiations are distinct biochemical processes.
authors.affiliation: Virology Laboratory, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
authors.forename: R C
authors.initials: RC
authors.lastname: Herman
mesh_terms: D000818:Animals; D002460:Cell Line; D007700:Kinetics; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D010750:Phosphoproteins / Q000235:genetics*; D010944:Plants / Q000378:metabolism; D012314:RNA Cap Analogs / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics*; D012156:Reticulocytes / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*
chemical_list: D010750:Phosphoproteins; D012314:RNA Cap Analogs; D012333:RNA, Messenger