Molecular cloning and expression in E. coli of a Salmonella typhi porin gene.
Immunoscreening of a Salmonella typhi cosmid library in E. coli allowed the detection of clones producing a 36 kDa porin from S. typhi. The gene is efficiently expressed in an E. coli porin-less mutant and the protein is exported to the outer membrane envelope. Two clones which markedly differ in their level of expression have been isolated.
Zaror
I
I
Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Santiago.
Gómez
I
I
Castillo
G
G
Yudelevich
A
A
Venegas
A
A
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D008970:Molecular Weight; D018272:Porins; D012485:Salmonella typhi / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D018272:Porins; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases