Genetic stability of African swine fever virus grown in monkey kidney cells. Brief report.
Viral DNA subpopulations were produced when the ASFV was grown in monkey kidney MS cells. They were detected after 44 passages but not during the first 14 passages or in the unadapted ASFV E 70 strain grown in pig leukocytes. Different viral variants were isolated and their genomes were characterized. Restriction enzyme site variations were detected in both terminal fragments, Cla I-M and Sal I-F, and in the internal fragments Clal-O and Sma I-H. These variations result in changes in the size of the viral genome which ranges from 156 Kbp to 170 Kbp.
Santurde
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Departamento de Patologie Animal I, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Ruiz Gonzalvo
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Carnero
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ME
Tabarés
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D000358:African Swine Fever Virus / Q000235:genetics* / Q000254:growth & development; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D000882:Haplorhini; D007497:Iridoviridae / Q000235:genetics*; D007668:Kidney
D004279:DNA, Viral; D004262:DNA Restriction Enzymes