Contribution of different GC-motifs to the control of simian virus 40 late promoter activity.
During the course of lytic infection the 21-bp repeat region regulates differentially the late gene expression; a mutant deleted for this region expresses late genes either to a higher level in the absence of T antigen or to a lower level in the late phase of infection as compared to wild type (23). By analysing a series of clustered point mutations generated within the GC-motifs we show that i) mutations within motifs I and II stimulate late transcription two to three-fold, suggesting that competition for transcription machinery between early-early and late promoters is mediated by these two motifs, ii) after viral replication, simultaneous mutations within motifs IV, V and VI decrease to 23% the efficiency of late transcription, indicating that these motifs are elements of the late promoter. Moreover comparison of results presented in this paper with results published by Barrera-Saldana et al. strongly suggest that late-early and late promoters are regulated in a similar manner.
Ernoult-Lange
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M
Laboratoire d'Oncologie Moléculaire, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Omilli
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May
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D000818:Animals; D001482:Base Composition; D002460:Cell Line; D003596:Cytosine; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D006147:Guanine; D009154:Mutation; D009710:Nucleotide Mapping; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D012333:RNA, Messenger; D006147:Guanine; D003596:Cytosine; D004262:DNA Restriction Enzymes