Expression of proteins essential for IS1 transposition: specific binding of InsA to the ends of IS1.
The insertion sequence IS1 displays a complex array of open reading frames (ORF). In an attempt to identify those which encode polypeptide products, we have systematically placed each ORF under the control of the P1 promoter of phage lambda. In the expression system we used, only the product of the insA gene was present in high enough amounts to be detected by polyacrylamide gel electrophoresis. The production of InsA was further increased in a first codon hook-up to phage T7 transcriptional and translational initiation signals. Cell extracts from InsA overproducers display a DNA binding activity specific for the ends of IS1. This activity was identified as the InsA protein itself.
Zerbib
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Centre de Recherche de Biochimie et Genetique Cellulaire du C.N.R.S., Toulouse, France.
Jakowec
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M
Prentki
P
P
Galas
D J
DJ
Chandler
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004251:DNA Transposable Elements