Establishment of a parathyroid hormone-like factor-producing esophageal carcinoma cell line (EC-GI).
A new cell line, designated EC-GI, was established from a 65-year-old patient with esophageal carcinoma who developed humoral hypercalcemia. The original tumor as well as the cell line caused marked hypercalcemia in tumor-bearing nude mice, in which a marked increase in osteoclastic bone resorption was demonstrated. The conditioned medium of EC-GI cells contained potent bone resorbing activity which stimulated cyclic AMP production in parathyroid hormone (PTH)-responsive osteoblast-like cells (ROS 17/2.8). EC-GI cells will be useful for characterization and purification of the PTH-like factor responsible for humoral hypercalcemia of malignancy.
Sato
K
K
Department of Medicine, Tokyo Women's Medical College.
Kasono
K
K
Ohba
Y
Y
Yashiro
T
T
Fujii
Y
Y
Yoshida
M A
MA
Tsushima
T
T
Shizume
K
K
D001681:Biological Assay; D001862:Bone Resorption; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D000242:Cyclic AMP / Q000096:biosynthesis; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D006934:Hypercalcemia / Q000209:etiology; D007621:Karyotyping; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D010281:Parathyroid Hormone; D000242:Cyclic AMP