The effects of cholesterol on lateral diffusion and vertical fluctuations in lipid bilayers. An electron-electron double resonance (ELDOR) study.
Electron-electron double resonance (ELDOR) and saturation-recovery spectroscopy employing 14N:15N stearic acid spin-label pairs have been used to study the effects of cholesterol on lateral diffusion and vertical fluctuations in lipid bilayers. The 14N:15N continuous wave electron-electron double resonance (CW ELDOR) theory has been developed using rate equations based on the relaxation model. The collision frequency between 14N-16 doxyl stearate and 15N-16 doxyl stearate, WHex (16:16), is indicative of lateral diffusion of the spin probes, while the collision frequency between 14N-16 doxyl stearate and 15N-5 doxyl stearate, WHex (16:5), provides information on vertical fluctuations of the 14N-16 doxyl stearate spin probe toward the membrane surface. Our results show that: (a) cholesterol decreases the electron spin-lattice relaxation time Tle of 14N-16 doxyl stearate spin label in dimyristoylphosphatidylcholine (DMPC) and egg yolk phosphatidylcholine (egg PC). (b) Cholesterol increases the biomolecular collision frequency WHex (16:16) and decreases WHex (16:5), suggesting that incorporation of cholesterol significantly orders the part of the bilayer that it occupies and disorders the interior region of the bilayer. (c) Alkyl chain unsaturation of the host lipid moderates the effect of cholesterol on both vertical fluctuations and lateral diffusion of 14N-16 doxyl stearate. And (d), there are marked differences in the effects of cholesterol on lateral diffusion and vertical fluctuations between 0-30 mol% and 30-50 mol% of cholesterol that suggest an inhomogeneous distribution of cholesterol in the membrane.
Yin
J J
JJ
National Biomedical ESR Center, Department of Radiology, Medical College of Wisconsin, Milwaukee 53226.
Feix
J B
JB
Hyde
J S
JS
D002784:Cholesterol; D003497:Cyclic N-Oxides; D004058:Diffusion; D004134:Dimyristoylphosphatidylcholine; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers; D008433:Mathematics; D008954:Models, Biological; D009587:Nitrogen Isotopes; D009590:Nitrogen Radioisotopes; D013113:Spin Labels
D003497:Cyclic N-Oxides; D008051:Lipid Bilayers; D009587:Nitrogen Isotopes; D009590:Nitrogen Radioisotopes; D013113:Spin Labels; C032363:16-nitroxystearic acid; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine