title: A simplified plaque assay for varicella vaccine.
abstract: A simple and accurate plaque assay is described for potency testing of attenuated varicella vaccine. Assays were performed on the African green monkey kidney continuous cell line CV-1, in multidish-plates, under a semi-solid carboxymethylcellulose overlay. The test is economical and yields accurate individual titre estimates, the reliability of which may be assessed by parallel titration of reference preparations.
authors.affiliation: Institut d' Hygiene et d' Epidemiologie, Departement Microbiologie, Brussels, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Husson-van Vliet
authors.forename: G
authors.initials: G
authors.lastname: Colinet
authors.forename: F
authors.initials: F
authors.lastname: Yane
authors.forename: P
authors.initials: P
authors.lastname: Lemoine
mesh_terms: D000818:Animals; D002460:Cell Line; D019433:Chickenpox Vaccine; D014645:Herpesvirus 3, Human / Q000254:growth & development / Q000276:immunology*; D012044:Regression Analysis; D010948:Viral Plaque Assay / Q000379:methods; D014765:Viral Vaccines / Q000592:standards*
chemical_list: D019433:Chickenpox Vaccine; D014765:Viral Vaccines