title: Immunoaffinity purification and characterization of nucleotide pyrophosphatase from human placenta.
abstract: Nucleotide pyrophosphatase [EC 3.6.1.9] was purified to homogeneity from human placenta using a monoclonal antibody affinity column. By sodium dodecylsulfate--polyacrylamide gel electrophoresis, the purified enzyme showed a major band at a molecular size of 130 K. The enzyme was a glycoprotein with N-linked oligosaccharides consisting of both complex- and oligomannoside-types. Substrate specificity to hydrolyze phosphodiester and phosphosulfate linkages as well as other properties were similar to those of nucleotide pyrophosphatase and phosphodiesterase from other sources.
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yano
authors.forename: K
authors.initials: K
authors.lastname: Horie
authors.forename: R
authors.initials: R
authors.lastname: Kanamoto
authors.forename: H
authors.initials: H
authors.lastname: Kitagawa
authors.forename: I
authors.initials: I
authors.lastname: Funakoshi
authors.forename: I
authors.initials: I
authors.lastname: Yamashina
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology*; D011755:Pyrophosphatases / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D006023:Glycoproteins; D011755:Pyrophosphatases; C021899:nucleotide pyrophosphatase