[Cloning of terminal fragments of the avian adenovirus CELO genome and isolation of a recombinant plasmid carrying the viral oncogene].
The terminal fragment of avian adenovirus CELO has been cloned in a plasmid vector. The obtained recombinant plasmid pCBE1 carries the terminal BamHI-E fragment of CELO DNA. Transfection of a nonpermissive culture of Rat2 cell line by the plasmid DNA results in formation of transformation focuses. The cloned BamHI-E fragment of CELO DNA is concluded to contain the viral oncogene. Thus, the CELO genome region deriving the BamHI-E fragment is "left".
Krugliak
V A
VA
Akopian
T A
TA
Naroditskiĭ
B S
BS
Kartashova
I M
IM
Shevliagin
V Ia
VIa
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000259:Aviadenovirus / Q000235:genetics*; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005814:Genes, Viral; D009857:Oncogenes; D010957:Plasmids; D011995:Recombination, Genetic
D004262:DNA Restriction Enzymes
Klonirovanie kontsevykh fragmentov genoma adenovirusa ptits CELO i poluchenie rekombinantnoĭ plazmidy, nesushcheĭ virusnyĭ onkogen.