Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium.
We determined the complete nucleotide sequence of the 6354-base-pair plasmid pHV2 of the archaebacterium Halobacterium volcanii. This plasmid is present in approximately six copies per chromosome. We have generated a strain, H. volcanii WFD11, cured of pHV2 by treatment of liquid cultures with ethidium bromide. We describe PEG-mediated transformation of H. volcanii WFD11 with intact pHV2 and with a form of pHV2 marked by a 93-base-pair deletion generated in vitro.
Charlebois
R L
RL
Department of Biochemistry, Dalhousie University, Halifax, NS, Canada.
Lam
W L
WL
Cline
S W
SW
Doolittle
W F
WF
D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006217:Halobacterium / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes