title: The structure and function of the integrated polyoma virus DNA in 82-rat and 53-rat transformed cells.
abstract: Integrated viral sequences and adjacent cellular sequences from the polyoma virus (Py)-transformed 53-Rat and 82-Rat cell lines which contain two and three partial early regions respectively, each in a single viral insert, have been molecularly cloned. Each of the cloned partial early regions have been subcloned and assessed with regard to their transcription, translation products (T antigens, T Ags) and biological activity including their transforming ability. The 53-Rat 5.3 kb EcoRI fragment is an intact Py EcoRI linear genome (derived from within the tandem duplicated sequences) which transforms rat cells with high efficiency and produces infectious virus when circularized and transfected into mouse cells. The 82-Rat cell line expresses three novel T Ag species of 63K, 40K and 32K in addition to the Py middle and small T Ags. The 63K protein was found to be a truncated form of large T Ag produced as the result of an addition/deletion in early region B sequences unique to large T Ag. The 40K and 32K proteins are hybrid viral-cellular middle and large T Ags respectively, which are expressed from early region A that has been truncated by recombination with rat cellular DNA. Differences in the nuclear and cytoplasmic location of the different 82-Rat early region RNAs are due to RNA stability and/or transport from the nucleus to the cytoplasm most likely as a result of different cellular sequences at their 3' ends. Finally no common structural feature or sequence specificity was observed at the virus-host DNA joins of the two cell lines.
authors.affiliation: Department of Tumour Genetics, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, U.K.
authors.forename: H E
authors.initials: HE
authors.lastname: Ruley
authors.forename: F
authors.initials: F
authors.lastname: Chaudry
authors.forename: M
authors.initials: M
authors.lastname: Read
authors.forename: L
authors.initials: L
authors.lastname: Lania
authors.forename: M
authors.initials: M
authors.lastname: Fried
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D001483:Base Sequence; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics*; D014176:Protein Biosynthesis; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D012984:Software; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI