[Identification of HLA B27 antigen by flow cytometry].
Cytofluorograph analysis after immunofluorescence with monoclonal antibodies can be useful for various antigens identification on the surface of cell membrane. This technic is described for antigen HLA B27 identification. This HLA antigen is known indeed to be linked to rhumatismal diseases. The fluorescence histograms obtained, allow to differentiate easily subjects which have HLA B27 phenotype and subjects which are HLA cross linked.
Fizet
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Service d'Immunologie cellulaire, Centre Régional de Transfusion Sanguine, Bordeaux.
Hitte
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C
Ferrer
A M
AM
Vezon
G
G
D000911:Antibodies, Monoclonal / Q000276:immunology; D003168:Complement Fixation Tests; D004311:Double-Blind Method; D005434:Flow Cytometry / Q000379:methods*; D015796:HLA-B27 Antigen / Q000032:analysis* / Q000276:immunology; D006801:Humans; D010641:Phenotype; D012216:Rheumatic Diseases / Q000175:diagnosis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015796:HLA-B27 Antigen
Identification de l'antigène HLA B27 par cytométrie de flux.