title: Controlled degradation of yeast tRNAPhe by spleen phosphodiesterase in the presence of ethidium bromide.
abstract: Degradation of yeast tRNAPhe with spleen phosphodiesterase in the presence of ethidium bromide has been studied. It was found that in the presence of the intercalating dye, the digestion is halted after a limited number of nucleotides is removed. Possible explanations of the observed phenomenon in connection with tRNA-ethidium bromide complex formation are discussed.
authors.affiliation: Polish Academy of Sciences, Department of Bioorganic Chemistry, Boczna.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Lesnikowski
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium / Q000494:pharmacology*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D013154:Spleen / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D010727:Phosphoric Diester Hydrolases; D004996:Ethidium