Passive transfer of myasthenia gravis by immunoglobulins: lack of correlation between AChR with antibody bound, acetylcholine receptor loss and transmission defect.
The effects of serum Ig from 7 myasthenia gravis patients on neuromuscular transmission was investigated by passive transfer to mice. A protocol of 60 mg/day for 3 days produced mouse serum levels of anti-mouse AChR that were similar to those in the MG patients, and resulted in corresponding levels (2-76%) of mouse muscle AChR with antibody bound in situ. However, AChR loss was only greater than 20% with one MG preparation. Nevertheless, there was a marked neuromuscular defect in mice injected with 3 preparations which did not necessarily correlate with the degree of AChR loss or the amount of AChRs with antibody bound in situ. We conclude that in some MG patients part of the defect in neuromuscular transmission may result from antibodies binding to other components of the neuromuscular junction.
Mossman
S
S
Department of Neurological Science, Royal Free Hospital School of Medicine, London, U.K.
Vincent
A
A
Newsom-Davis
J
J
D000293:Adolescent; D000328:Adult; D000818:Animals; D003165:Complement System Proteins / Q000494:pharmacology; D003472:Curare / Q000494:pharmacology; D003964:Diaphragm / Q000294:innervation / Q000503:physiopathology; D005260:Female; D006801:Humans; D007116:Immunization, Passive; D007136:Immunoglobulins / Q000097:blood*; D008297:Male; D051379:Mice; D008875:Middle Aged; D009045:Motor Endplate / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D009046:Motor Neurons / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D009157:Myasthenia Gravis / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D009469:Neuromuscular Junction / Q000276:immunology*; D011950:Receptors, Cholinergic / Q000276:immunology* / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects; D013997:Time Factors
D007136:Immunoglobulins; D011950:Receptors, Cholinergic; D003472:Curare; D003165:Complement System Proteins