ack::Mu d1-8 (Apr lac) operon fusions of Salmonella typhimurium LT2.
Several ack::Mu d1-8 insertions were isolated from Salmonella typhimurium on MacConkey-glucose-trimethylamine oxide medium. They could not produce gas from glucose in the absence of exogenous formate. Two of the mutants expressed beta-galactosidase activity and were shown to be ack::Mu d1-8 fusions by genetic analysis. These insertions were characterized and located on the genetic map at the ack locus by co-transduction with zei::Tn10. The gene order zei::Tn10-ack-pta was established. The direction of transcription of ack was clockwise on the genetic map. Expression of the lac operon in ack::Mu d1-8 was increased twofold by anaerobiosis. Addition of formate, pyruvate, and acetate did not affect the anaerobic expression of the lac operon in ack::Mu d1-8.
Kwan
H S
HS
Department of Biology, Chinese University of Hong Kong, Shatin, N.T.
Chui
H W
HW
Wong
K K
KK
D000084:Acetate Kinase / Q000235:genetics*; D000693:Anaerobiosis; D010583:Bacteriophage mu / Q000235:genetics; D002874:Chromosome Mapping; D003470:Culture Media; D004252:DNA Mutational Analysis; D007763:Lac Operon; D009876:Operon; D010641:Phenotype; D010770:Phosphotransferases / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics*; D012641:Selection, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D003470:Culture Media; D010770:Phosphotransferases; D000084:Acetate Kinase; D001616:beta-Galactosidase