title: Cloning of the gene encoding quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus: evidence for the presence of a second enzyme.
abstract: We cloned the gene coding for the quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus. This clone complements gdh mutations in A. calcoaceticus, Pseudomonas aeruginosa, and Escherichia coli. The gene codes for a protein with an Mr of 83,000. Evidence is presented for the presence of two different glucose dehydrogenase enzymes in A. calcoaceticus: a protein with an Mr of 83,000 and a dimer of two identical subunits with an Mr of 50,000.
authors.affiliation: Laboratory of Molecular Genetics, University of Leiden, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Cleton-Jansen
authors.forename: N
authors.initials: N
authors.lastname: Goosen
authors.forename: T J
authors.initials: TJ
authors.lastname: Wenzel
authors.forename: P
authors.initials: P
authors.lastname: van de Putte
mesh_terms: D000150:Acinetobacter / Q000201:enzymology / Q000235:genetics*; D002237:Carbohydrate Dehydrogenases / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005948:Glucose Dehydrogenases / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics
chemical_list: D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone); D004262:DNA Restriction Enzymes