Superoxide as a signal for increase in intracellular pH.
We examined the role of superoxide in the increase in intracellular pH (pHi) of human histiocytic leukemia U937 cells treated with 4 beta-phorbol-12,13-didecanoate (4 beta-PDD) or serum. 4 beta-PDD or serum induced a rapid increase in pHi, and antioxidants such as superoxide dismutase (SOD), vitamin E, and butylated hydroxyanisole (BHA) were found to inhibit the amiloride-sensitive increase in pHi induced by 4 beta-PDD. SOD inhibited the increase in pHi caused by serum, and essentially the same was found in concanavalin A-stimulated mouse thymocytes. Also, a superoxide-generating system, xanthine-xanthine oxidase (X-XOD), increased pHi of U937 cells as much as 4 beta-PDD or serum. From these findings, it appears that superoxide is the basis for the modulation of pHi.
Shibanuma
M
M
Department of Cancer Cell Research, University of Tokyo, Japan.
Kuroki
T
T
Nose
K
K
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001769:Blood; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002460:Cell Line; D006863:Hydrogen-Ion Concentration; D007951:Leukemia, Myeloid / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology
D010703:Phorbol Esters; D013481:Superoxides; D014810:Vitamin E; C008876:phorbol-12,13-didecanoate; D002083:Butylated Hydroxyanisole; D000584:Amiloride; D013482:Superoxide Dismutase