title: Chemical reactions within DNA duplexes. Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent.
abstract: Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in aqueous solution. Optimum conditions for this vigorous and effective reaction were developed. CNBr proved to be useful for incorporation of phosphoramidate or pyrophosphate internucleotide bonds in DNA duplexes.
authors.affiliation: A.N. Belozersky Laboratory, Moscow State University, USSR.
authors.forename: N I
authors.initials: NI
authors.lastname: Sokolova
authors.forename: D T
authors.initials: DT
authors.lastname: Ashirbekova
authors.forename: N G
authors.initials: NG
authors.lastname: Dolinnaya
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
mesh_terms: D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003488:Cyanogen Bromide / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D011088:DNA Ligases / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D009838:Oligodeoxyribonucleotides / Q000378:metabolism
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D011088:DNA Ligases; D003488:Cyanogen Bromide