Is calmodulin inhibition involved in shape transformations induced by amphiphiles in erythrocytes?
Potent antihaemolytic and shape transforming amphiphilic compounds were studied for their ability to inhibit calmodulin-activated phosphodiesterase activity. Some echinocytogenic and stomatocytogenic amphiphiles were potent calmodulin inhibitors. The most potent echinocytogenic and stomatocytogenic amphiphiles, however, had no or only weak inhibitory effect. Our results show that there is no causal relationship between the ability of amphiphiles to induce antihaemolysis or shape transformations in erythrocytes and their ability to inhibit calmodulin-activated phosphodiesterase activity, and it is suggested that calmodulin is not involved in shape transformations induced by amphiphiles.
Isomaa
B
B
Department of Biology, Abo Akademi, Turku, Finland.
Engblom
A C
AC
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000097:blood; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002746:Chlorpromazine / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D007700:Kinetics
D002121:Calcium Channel Blockers; D002147:Calmodulin; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D002746:Chlorpromazine