title: Molecular structure of the cell wall receptor for killer toxin KT28 in Saccharomyces cerevisiae.
abstract: The adsorption of the yeast killer toxin KT28 to susceptible cells of Saccharomyces cerevisiae was prevented by concanavalin A, which blocks the mannoprotein receptor. Certain mannoprotein mutants of S. cerevisiae that lack definite structures in the mannan of their cell walls were found to be resistant to KT28, whereas the wild-type yeast from which the mutants were derived was susceptible. Isolated mannoprotein from a resistant mutant was unable to adsorb killer toxin. By comparing the resistances of different mannoprotein mutants, information about the molecular structure of the receptor was obtained. At least two mannose residues have to be present in the side chains of the outer chain of the cell wall mannan, whereas the phosphodiester-linked mannose group is not essential for binding and the subsequent action of killer toxin KT28.
authors.affiliation: Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität Mainz, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schmitt
authors.forename: F
authors.initials: F
authors.lastname: Radler
mesh_terms: D000327:Adsorption; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D003208:Concanavalin A / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D055646:Killer Factors, Yeast; D008562:Membrane Glycoproteins; D009154:Mutation; D009183:Mycotoxins / Q000378:metabolism* / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D013104:Spheroplasts / Q000187:drug effects
chemical_list: D005656:Fungal Proteins; D006023:Glycoproteins; D055646:Killer Factors, Yeast; D008562:Membrane Glycoproteins; D009183:Mycotoxins; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; C025363:mannoproteins; D003208:Concanavalin A