[Leukocyte removal in a closed system of human red-cell concentrates: a technic coupled with the automated extraction of the buffy coat using a sterile connector from a filtration kit].
A technique, integrally run in a closed system, for leucocyte depletion of human red cell concentrates is described. It associates two complementary processes: buffy-coat removal and filtration. The first step is carried out with an automated system for blood component preparation (Compomat, NPBI); its efficiency is improved by a custom made blood collection set with ACD anticoagulant solution in the primary bag. The second step is simplified by a filtration kit requiring only one sterile connection for operation (SCD 312, Dupont de Nemours) and allowing a standardised rinsing of the filter. Quality control of 33 units so prepared shows principally: --an intensive leuko-depletion (4 logs) enabling leukocyte contamination to be kept below 10(6) per unit; --a moderate red cells loss (15 ml for the first step and 20 ml for the second one). This technique provides a permanently available and very pure blood component. Moreover it offers new potential for standardisation and mastery of quality control.
Angué
M
M
Centre Régional de Transfusion Sanguine de Dijon.
Chatelain
P
P
Richaud
P
P
Guignier
F
F
Domy
M
M
D001772:Blood Cell Count; D001781:Blood Component Removal / Q000295:instrumentation / Q000379:methods*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D017707:Erythrocyte Transfusion; D004912:Erythrocytes; D005374:Filtration / Q000295:instrumentation; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007962:Leukocytes
D006454:Hemoglobins
Déleucocytation en système clos des concentrés de globules rouges humains: une technique associant l'extraction automatisée du buffy-coat à la connexion stérile d'un kit de filtration.