Requirement for fos gene expression in the transcriptional activation of collagenase by other oncogenes and phorbol esters.
Transcription from the c-fos promoter and from minimal promoter constructs carrying the phorbol ester-responsive element [12-O-tetradecanoylphorbol-13-acetate (TPA) responsive element (TRE)] corresponding to the sequence in the human collagenase gene is activated by elevated levels of the oncogene products v-src, c-Ha-ras, activated c-Ha-ras, and v-mos, as well as by phorbol ester. Elevated c- or v-fos expression stimulates TRE-dependent transcription but represses the c-fos promoter. Antisense fos sequences abolish basal and induced transcription from TRE constructs and derepress the c-fos promoter. These results establish a key role for fos in signal transduction and implicate the fos protein as a trans-activating and -repressing molecule.
Schönthal
A
A
Kernforschungszentrum Karlsruhe, Universität Karlsruhe, Federal Republic of Germany.
Herrlich
P
P
Rahmsdorf
H J
HJ
Ponta
H
H
D004790:Enzyme Induction; D005786:Gene Expression Regulation; D006801:Humans; D003012:Microbial Collagenase / Q000096:biosynthesis / Q000235:genetics*; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D010703:Phorbol Esters / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D017231:Proto-Oncogene Proteins c-mos; D016283:Proto-Oncogene Proteins p21(ras); D012191:Retroviridae Proteins / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D010703:Phorbol Esters; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012191:Retroviridae Proteins; D015688:Oncogene Protein pp60(v-src); D017231:Proto-Oncogene Proteins c-mos; D003012:Microbial Collagenase; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)