Interaction of the mouse chromosomal protein HMG-I with the 3' ends of genes in vitro.
High affinity mouse HMG-I binding sites have been distinguished from other (A+T)-rich sequences using band competition assays. These sites have been found 3' to the coding regions of a variety of genes. For the herpes simplex virus thymidine kinase and minute virus of mice genes, high affinity HMG-1 binding sites were further localized to the functional polyadenylation signal by DNase I footprinting. These results suggest that HMG-I may function at the 3' ends of genes in vivo.
Russnak
R H
RH
Department of Biochemistry, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Candido
E P
EP
Astell
C R
CR
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003850:Deoxyribonuclease I / Q000378:metabolism; D005796:Genes; D006609:High Mobility Group Proteins / Q000378:metabolism*; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics; D008969:Molecular Sequence Data; D011067:Poly dA-dT / Q000378:metabolism; D018139:Simplexvirus / Q000201:enzymology; D013937:Thymidine Kinase / Q000378:metabolism
D006609:High Mobility Group Proteins; D011067:Poly dA-dT; D013937:Thymidine Kinase; D003850:Deoxyribonuclease I