High level expression of the EcoP1 modification methylase gene and characterisation of the gene product.
We have cloned the gene coding for the EcoP1 modification methylase in an expression system based on the phage lambda pL promoter and the cI857-coded thermoinducible repressor. We have used this system to purify the enzyme on the 20-30-mg scale and have examined some of its enzymatic properties. The enzyme is a tetramer of Mr 72,000 subunits and is approx. 40% alpha-helical. Experiments with the methyl donor, S-adenosyl methionine, radioactively labelled in different positions indicate that a methyl group is transferred to the enzyme during the reaction in what is most likely a covalent bond.
Hornby
D P
DP
Department of Microbiology, University of Basel, Switzerland.
Müller
M
M
Bickle
T A
TA
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000235:genetics* / Q000302:isolation & purification; D015263:Deoxyribonucleases, Type III Site-Specific; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008780:Methyltransferases / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
C053885:DNA modification methylase EcoPI; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D004262:DNA Restriction Enzymes; C030432:endodeoxyribonuclease EcoP1; D015263:Deoxyribonucleases, Type III Site-Specific