An antibody and anti-idiotypic antibody against the extra signal peptide of pre-aspartate aminotransferase.
A peptide (extra signal peptide) comprising amino acids 1-29 of pig liver pre-mitochondrial aspartate aminotransferase (p-mAAT) was synthesized chemically. The peptide was found to block the import of rat liver p-mAAT into rat liver mitochondria. An antibody raised against the peptide immunoprecipitated rat liver p-mAAT synthesized in a rabbit reticulocyte cell-free translation system. These results suggested that the extra signal peptide sequence of p-mAAT is essential for import of p-mAAT into the mitochondria and that there is structural homology between the extra signal peptides of pig and rat liver p-mAAT. An anti-idiotypic antibody against the peptide was also prepared and purified by affinity chromatography on an Affi-Gel 10 anti-peptide IgG column and was then characterized.
Sakakibara
R
R
Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University, Japan.
Horio
Y
Y
Ishiguro
M
M
Kangawa
K
K
Matsuo
H
H
Wada
H
H
D000818:Animals; D001219:Aspartate Aminotransferases / Q000276:immunology / Q000378:metabolism*; D001667:Binding, Competitive; D001692:Biological Transport; D011232:Chemical Precipitation; D004792:Enzyme Precursors / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D021382:Protein Sorting Signals / Q000138:chemical synthesis / Q000276:immunology / Q000502:physiology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000502:physiology; D013552:Swine
D004792:Enzyme Precursors; D007130:Immunoglobulin Idiotypes; D021382:Protein Sorting Signals; D011956:Receptors, Cell Surface; D001219:Aspartate Aminotransferases