Ki-1+ cutaneous lymphoma. Gene rearrangement analysis of tumor cells in tissue and short-term culture of a patient.
Cutaneous malignant lymphomas developed in an 80-year-old man without any evidence of leukemic disseminations of lymphoma cells. Immunohistologic staining showed the expression of Leu-3a, Leu-4, Ki-1, Tac, and HLA-DR antigens on tumor cells in tissue and large lymphoid cells in long-term, interleukin 2-containing culture of tumor explants. DNA samples extracted from the skin tumors and cultured lymphoid cells showed a clonal rearrangement of T-cell receptor (TCR) gene C beta 1 with the molecular size of 9 kilobases. These findings suggested the T-cell origin of Ki-1+ cutaneous lymphoma cells and the occurrence of a clonal proliferation of tumor cells in culture.
Sugimoto
H
H
Department of Dermatology, Hamamatsu, Japan, University School of Medicine.
Nakayama
F
F
Yamauchi
T
T
Tokura
Y
Y
Iwatsuki
K
K
Takigawa
M
M
Yamada
M
M
Maeda
M
M
D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000032:analysis*; D002455:Cell Division; D002999:Clone Cells; D006801:Humans; D007158:Immunologic Techniques; D017730:Ki-1 Antigen; D008223:Lymphoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D011995:Recombination, Genetic; D012878:Skin Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000473:pathology
D000951:Antigens, Neoplasm; D017730:Ki-1 Antigen; D011948:Receptors, Antigen, T-Cell