title: Improvement of a procedure for preparing single smooth muscle cells from guinea pig tenia coli by purified collagenase and papain.
abstract: Procedures for isolating single smooth muscle cells from tenia coli of guinea pigs have been developed. The cells were isolated with a combination of highly purified collagenase and a small amount of strong peptidase, papain, in the presence of Ca. This combination resulted in approximately a 100-fold yield of the single cells as compared with the ordinary method in which only collagenase preparations contaminated with various peptidases were used. More than 95% of the single cells were viable when examined by the trypan blue exclusion technique. In addition, this combination greatly increased the responsiveness to agonists such as carbachol (CCh). A concentration of CCh for the half maximum response for this preparation was about one five-hundredth that of cells prepared by the ordinary method. This value was approximately one-quarter that of intact tissue. This preparation can be used to investigate the physiology, pharmacology, and biochemistry of smooth muscle.
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Maruyama
authors.forename: C
authors.initials: C
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: K
authors.initials: K
authors.lastname: Momose
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D003106:Colon / Q000166:cytology; D006168:Guinea Pigs; D008722:Methods; D003012:Microbial Collagenase / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000166:cytology*; D010206:Papain / Q000494:pharmacology*
chemical_list: D001285:Atropine; D002217:Carbachol; D010206:Papain; D003012:Microbial Collagenase