[Identification of virus-specific RNA in cells infected with the bovine leukemia virus by nucleic acid hybridization in situ].
Virus-specific RNA was detected in FLK cells producing bovine leukemia virus (BLV) by cDNA hybridization in situ. For calculation of the virus-specific RNA copy number in infected cells hybridization of 3H-cDNA BLV with the total cellular RNA in solution was used. FLK cells contain approximately 600 copies of virus-specific RNA per cell. Individual FLK cells producing BLV were identified by hybridization in situ. A successful identification of viral RNA in cells by hybridization in situ is possible if cells contain more than 100 copies of viral transcripts.
Brashishkite
D A
DA
Kiselev
F L
FL
D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D012756:Sheep; D012996:Solutions
D004279:DNA, Viral; D012367:RNA, Viral; D012996:Solutions
Identifikatsiia virus-spetsificheskikh RNK v kletkakh, zarazhennykh virusom leĭkoza krupnogo rogatogo skota, metodom gibridizatsii nukleinovykh kislot in situ.