A quenched-flow study of a receptor-triggered second messenger response: cyclic AMP burst elicited by isoproterenol in C6 glioma cell membranes.
Fast kinetic studies of cAMP accumulation in C6 cell membranes show a burst of cAMP after beta-adrenergic receptor stimulation by isoproterenol. This burst is no longer observed when the ATP present in membrane preparations is hydrolyzed, but can be restored by their preincubation in the presence of ATP-Mg. The size of the burst is much larger than the number of beta-adrenergic receptors and is of the same order of magnitude as the value reported for G proteins. Further characterization of the burst will allow studies of the functional interaction of receptor-adenylate cyclase components in C6 membranes.
Valeins
H
H
Institut de Biochimie Cellulaire et de Neurochimie, CNRS, Bordeaux, France.
Volker
T
T
Viratelle
O
O
Labouesse
J
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism*; D005910:Glioma; D007545:Isoproterenol / Q000494:pharmacology*; D007700:Kinetics; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000502:physiology*
D011943:Receptors, Adrenergic, beta; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D007545:Isoproterenol