Transformation by the oncogene v-fms: the effects of castanospermine on transformation-related parameters.
The effects of castanospermine on various parameters associated with transformation were examined in cells expressing the viral oncogene v-fms. Fischer rat embryo (FRE) cells transformed by the oncogene v-fms and grown in the presence of castanospermine reverted to a more normal cell morphology and accumulated fms protein within the endoplasmic reticulum. Treated cells attained contact inhibition of cell growth at a much lower cell density compared to the untreated controls. No effect of castanospermine on cell growth was observed for FRE cells transformed by a different oncogene v-fgr. Castanospermine-treated SM-FRE (v-fms transformed) cells reexpressed extracellular matrix fibronectin and exhibited an extensive actin-containing cytoskeleton similar to that of normal nontransformed FRE cells. Castanospermine treatment of SM-FRE cells resulted in a sixfold decrease in [3H]deoxyglucose uptake compared to that of the nonreverted SM-FRE cells. Again, no effect was observed in FRE cells transformed by the oncogene v-fgr (GR-FRE). These results further characterize the reversion caused by castanospermine and indicate that cell surface expression coordinately controls anchorage independent growth, cell morphology, contact inhibition of growth, and hexose uptake.
Nichols
E J
EJ
Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle, Washington 98104.
Manger
R
R
Hakomori
S I
SI
Rohrschneider
L R
LR
D000199:Actins / Q000032:analysis; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000187:drug effects*; D003593:Cytoplasm / Q000032:analysis; D003599:Cytoskeleton / Q000032:analysis; D005109:Extracellular Matrix / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D007212:Indolizines; D009856:Oncogene Proteins, Viral / Q000032:analysis; D009857:Oncogenes / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D012190:Retroviridae / Q000235:genetics*; D005253:Sarcoma Viruses, Feline / Q000235:genetics*; D054836:Indolizidines
D000199:Actins; D000470:Alkaloids; D005353:Fibronectins; D005947:Glucose; D007212:Indolizines; D009856:Oncogene Proteins, Viral; C037806:castanospermine; D054836:Indolizidines