Isolation of a simian virus 40-simian agent 12 recombinant papovavirus that synthesizes a hybrid large tumour antigen.
The simian virus 40 (SV40) mutant dl1055 carries a 55 base pair deletion in the viral early region coding sequences that causes premature termination of large tumour (T) antigen translation resulting in a protein fragment consisting of the amino-terminal 399 residues. The mutation renders the virus defective. We report the characterization of hyb4001, which was isolated as a single plaque following cotransfection of permissive cells with dl1055 DNA and the DNA of a related papovavirus, simian agent 12 (SA12). Hyb4001 arose by two homologous recombination events involving crossovers in regions of 7 and 12 base pairs of perfect homology between the two viruses. Hyb4001 synthesizes a hybrid T antigen with the amino-terminal 382 residues encoded by SV40, residues 383 to 449 encoded by SA12, and the carboxy-terminal 259 residues encoded by SV40.
Hunter
L A
LA
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
Pipas
J M
JM
D000595:Amino Acid Sequence; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003434:Crossing Over, Genetic; D003673:Defective Viruses / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D027382:Polyomaviridae; D012689:Sequence Homology, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000957:Antigens, Viral, Tumor