4-Nitrophenylalkaline phosphatase inhibition in muscular dystrophy.
The allosteric behaviour of 4-nitrophenylphosphatase from membrane erythrocytes was investigated in Duchenne muscular dystrophy (DMD) patients, in female carriers and in healthy controls. Cooperative type kinetics with a Hill coefficient of -2.19, -1.71 and -1.54 has been obtained from the inhibition by fluoride in controls, female carriers and Duchenne patients, respectively. Our observation supports the previously described membrane abnormalities in DMD erythrocytes and may extend then to female carriers.
Goldemberg
A L
AL
Dpto. de Biología, Fac. de Ciencias Exactas y Naturales, Mar del Plata, Argentina.
Sánchez
J J
JJ
García
A M
AM
Fernández
H
H
Fortunato
M
M
Trucco
R E
RE
D009597:4-Nitrophenylphosphatase / Q000037:antagonists & inhibitors*; D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009136:Muscular Dystrophies / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors*; D012737:Sex Factors
D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase