title: Characterization of secretory responses of a glucagon-producing In-R1-G9 cell line.
abstract: The In-R1-G9 cell line is one of the clones derived from the In-111-R1 hamster insulinoma cell line and produces glucagon. The secretory responses of In-R1-G9 cells were further examined to characterize the nature of the cells. Vincristine had no effect on glucagon secretion and colchicine enhanced glucagon secretion slightly after a short incubation. Two calmodulin inhibitors, trifluoperazine and chlorpromazine, did not affect glucagon secretion. Monensin at 10(-8) M suppressed glucagon secretion by 50%. Secretion of glucagon was calcium-dependent. The addition of A23187 to the incubation medium resulted in a 180% increase over control for 1 h and calcium deprivation from the medium suppressed glucagon secretion markedly. Theophylline, a phosphodiesterase inhibitor, caused a 230% increase in glucagon secretion. An experiment using cycloheximide suggested that newly synthesized glucagon appears in the medium at 30 min. This cell line should be useful for various experiments in many fields of research.
authors.affiliation: First Department of Medicine, School of Medicine, Fukuoka University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Ono
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Okeda
authors.forename: T
authors.initials: T
authors.lastname: Asano
authors.forename: R
authors.initials: R
authors.lastname: Takaki
mesh_terms: D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002999:Clone Cells; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000187:drug effects; D005934:Glucagon / Q000378:metabolism*; D007340:Insulinoma / Q000378:metabolism*; D008985:Monensin / Q000494:pharmacology; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D005934:Glucagon; D008985:Monensin; D003513:Cycloheximide; D003078:Colchicine; D002118:Calcium