Purification of envelope glycoproteins of human T cell lymphotropic virus type I (HTLV-I) by affinity chromatography.
The external envelope glycoprotein (gp46) and transmembrane glycoprotein (gp21) of human T-cell lymphotropic virus type I (HTLV-I) were isolated from lysates of HTLV-I-infected HUT-102 cells by affinity chromatography. Fifty ml aliquots of packed HUT-102 cells were extracted with 1% Triton X-100, and lysates were treated sequentially with an affinity column containing IgG from an HTLV-I+ human subject followed by chromatography of the bound fraction over a lentil lectin column. The identity of the purified envelope proteins was confirmed with a human monoclonal antibody (0.5 alpha) to gp46 and with rabbit antisera raised to a synthetic peptide from the C-terminus of gp21. Affinity-purified envelope glycoproteins were bound to microtiter wells and used in radioimmunoassay to detect murine and human anti-envelope antibodies to gp46 and gp21 molecules.
Palker
T J
TJ
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Clark
M E
ME
Sarngadharan
M G
MG
Matthews
T J
TJ
D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D002846:Chromatography, Affinity; D017977:Deltaretrovirus / Q000032:analysis* / Q000276:immunology; D015686:Gene Products, env; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D011863:Radioimmunoassay; D012191:Retroviridae Proteins / Q000276:immunology / Q000302:isolation & purification*; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins / Q000276:immunology / Q000302:isolation & purification*; D054299:env Gene Products, Human Immunodeficiency Virus
D000914:Antibodies, Viral; D015686:Gene Products, env; D007074:Immunoglobulin G; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; D054299:env Gene Products, Human Immunodeficiency Virus; C055265:gp21 protein, Human T-lymphotropic virus 1; C055266:gp46 protein, Human T-cell leukemia virus type I