Detection of antibodies to Sendai virus in mouse sera by enzyme-linked immunosorbent assay (ELISA).
Enzyme-linked immunosorbent assay (ELISA) for diagnosis of Sendai virus infection in mice was evaluated. A large-scale survey of infected mice showed that ELISA is approximately 100 times more sensitive than the hemagglutination-inhibition or complement-fixation test. Although a few SPF mice showed false-positive reactions at a serum dilution of 1:40, further dilution to 1:80 eliminated the non-specific reaction. It was shown that ELISA is a highly satisfactory method for examination of Sendai virus infection in mice.
Suzuki
E
E
Department of Veterinary Science, National Institute of Health, Tokyo.
Noguchi
A
A
Takeda
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000302:isolation & purification*; D052160:Benzothiazoles; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006735:Horseradish Peroxidase; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D013451:Sulfonic Acids
D000914:Antibodies, Viral; D052160:Benzothiazoles; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006735:Horseradish Peroxidase