title: Characterization of the transformation properties of Shope fibroma virus.
abstract: To investigate if Shope fibroma virus (SFV), a leporipoxvirus that induces benign tumors in adult rabbits, can trigger the second step of carcinogenesis in vitro or malignant transformation, an already immortalized rabbit cell line (SIRC) was inoculated with ultraviolet-irradiated virus. The resulting cell transformants displayed the characteristic properties of the malignant phenotype: lack of infectious particles, low serum requirement, high efficiency of cloning, resistance to superinfection, presence of viral DNA sequences in the nucleus, expression of viral proteins and induction of tumors in rabbits. However, this transformation was not stable since in all cell lines studied, a loss of the malignant phenotype was recorded close to the 50th passage. To assess the oncogenic potential of SFV, NIH 3T3 cells were transfected with SFV DNA. The results of these experiments indicate that SFV DNA can induce the formation of foci in certain NIH 3T3 cell lines. Taken together these results support the notion that SFV can elicit the transformation of cells in vitro.
authors.affiliation: Center for Experimental Cell Biology, Mollie B. Roth Laboratory, Mount Sinai School of Medicine, City University of New York, NY 10029.
authors.forename: K M
authors.initials: KM
authors.lastname: Obom
authors.forename: B G
authors.initials: BG
authors.lastname: Pogo
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000209:etiology*; D002472:Cell Transformation, Viral / Q000187:drug effects / Q000528:radiation effects; D003043:Cocarcinogenesis; D004279:DNA, Viral / Q000235:genetics; D005347:Fibroblasts; D012776:Fibroma Virus, Rabbit / Q000235:genetics / Q000472:pathogenicity* / Q000528:radiation effects; D051379:Mice; D009374:Neoplasms, Experimental / Q000209:etiology; D011212:Poxviridae / Q000472:pathogenicity*; D011817:Rabbits; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection; D014466:Ultraviolet Rays
chemical_list: D004279:DNA, Viral; D013755:Tetradecanoylphorbol Acetate