Regulated expression of stably transfected herpes simplex virus thymidine kinase genes in continuous cell lines expressing a temperature-sensitive mutant form of the immediate-early protein ICP4.
We stably transfected the herpes simplex virus type 1 thymidine kinase gene into a continuous cell line expressing a temperature-sensitive form of the viral immediate-early protein ICP4. In these cells, expression of the thymidine kinase gene was regulated in a temperature-sensitive manner, partially reproducing the controls that operate during a viral infection.
Pasick
J M
JM
Pathology Department, McMaster University, Hamilton, Ontario, Canada.
Smiley
J R
JR
D005786:Gene Expression Regulation; D017874:Immediate-Early Proteins; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology*
C050678:IE1 protein, Human herpesvirus 1; D017874:Immediate-Early Proteins; D014764:Viral Proteins; C048925:herpes simplex virus, type 1 protein ICP4; D013937:Thymidine Kinase