title: The histone H3 and H4 mRNAs are polyadenylated in maize.
abstract: Northern blot analysis revealed that the histone H3 and H4 mRNAs are of unusual large size in germinating maize embryos. S1-mapping experiments show that the 3'-untranslated regions of the mRNAs transcribed from 3 H3 and 2 H4 maize genes previously described are much longer than in the non-polyadenylated histone mRNAs which represent a major class in animals. Moreover, oligo d(T) cellulose fractionation of RNAs isolated at different developmental stages indicates that more than 99% of the maize H3 and H4 mRNAs are polyadenylated. A putative polyadenylation signal is present in all five genes 17 to 27 nucleotides before the 3'-ends of the mRNAs.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
authors.forename: N
authors.initials: N
authors.lastname: Chaubet
authors.forename: M E
authors.initials: ME
authors.lastname: Chaboute
authors.forename: B
authors.initials: B
authors.lastname: Clément
authors.forename: M
authors.initials: M
authors.lastname: Ehling
authors.forename: G
authors.initials: G
authors.lastname: Philipps
authors.forename: C
authors.initials: C
authors.lastname: Gigot
mesh_terms: D001483:Base Sequence; D004720:Endonucleases; D006657:Histones / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D010944:Plants / Q000235:genetics*; D011061:Poly A / Q000032:analysis*; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D003313:Zea mays / Q000235:genetics
chemical_list: D006657:Histones; D012333:RNA, Messenger; D011061:Poly A; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases