title: Characterization of Erwinia chrysanthemi extracellular proteases: cloning and expression of the protease genes in Escherichia coli.
abstract: Erwinia chrysanthemi, a phytopathogenic enterobacterium, secretes three antigenically and structurally distinct proteases, A, B, and C and produces a protease inhibitor, a low-molecular-weight, heat-stable protein which remains mostly intracellular and which binds specifically to the A, B, and C proteases. The structural genes for proteases A, B, and C and for the inhibitor are clustered on a ca. 40-kilobase DNA fragment present in cosmid pEW4. Escherichia coli strains harboring pEW4 secrete the three proteases into the medium during the exponential phase of growth, without intracellular accumulation and in the absence of detectable cell lysis. An 8.5-kilobase EcoRI fragment derived from the cosmid encodes proteases B and C and the inhibitor as well as functions involved in the synthesis or secretion (or both) of the proteases. The inhibitor is not required for protease synthesis or secretion.
authors.affiliation: Unité de Génétique Moléculaire, Institut Pasteur, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Wandersman
authors.forename: P
authors.initials: P
authors.lastname: Delepelaire
authors.forename: S
authors.initials: S
authors.lastname: Letoffe
authors.forename: M
authors.initials: M
authors.lastname: Schwartz
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004885:Erwinia / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D007700:Kinetics; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D011480:Protease Inhibitors / Q000032:analysis; D014158:Transcription, Genetic
chemical_list: D011480:Protease Inhibitors; D004262:DNA Restriction Enzymes; D010447:Peptide Hydrolases