Tissue plasminogen activator mRNA in murine tissues.
The urokinase-type and tissue-type plasminogen activators are the two enzymes found in mammals, which specifically convert the zymogen plasminogen to plasmin. Using cDNA probes, we have assayed for the presence of the two types of plasminogen activator mRNAs in murine tissues. We demonstrate that tissue-type plasminogen activator mRNA can be detected in a wide variety of tissues. In contrast, the accumulation of urokinase-type plasminogen activator mRNA is observed in only a few of the tissues analyzed. Using an S1 nuclease assay, we demonstrate that the tPA mRNA detected contains the complete sequences encoding the non-protease finger, growth-factor and kringle domains.
Rickles
R J
RJ
Department of Microbiology, State University of New York, Stony Brook 11794.
Strickland
S
S
D000818:Animals; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014018:Tissue Distribution; D010959:Tissue Plasminogen Activator / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D010959:Tissue Plasminogen Activator