title: A class of Escherichia coli proteins controlled by the hflA locus.
abstract: The HflA protein of Escherichia coli is critical for the choice of the lytic or lysogenic pathway by bacteriophage lambda. To investigate whether HflA plays a regulatory role in E. coli, we used two-dimensional gel electrophoresis to compare the distribution of E. coli proteins in hflA+ and hflA- cells. We found at least 13 proteins that are present in hflA- strains, but absent or very low in hflA+ strains. This observation indicates that HflA might be involved in regulation of a large class of E. coli proteins. Because of prior work implicating the Crp/cAMP system in Hfl-mediated regulation of lambda, we also studied the distribution of E. coli proteins in cya- strains unable to synthesize cAMP. Some of the same proteins found in hflA- are elicited by the addition of cAMP to cya- cells.
authors.affiliation: Department of Molecular Biology, University of California, Berkeley 94720.
authors.forename: H H
authors.initials: HH
authors.lastname: Cheng
authors.forename: H
authors.initials: H
authors.lastname: Echols
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005796:Genes; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; D000242:Cyclic AMP