title: Effect of parasympathetic denervation on K+ release by rat parotid slices.
abstract: 1. By measuring the release of 86Rb, potassium movement was compared in parasympathectomized (Px) and contralateral control parotid gland slices three weeks following surgical denervation. 2. Carbachol and phenylephrine elicited a biphasic increase in 86Rb release that was dose related and could be blocked by atropine and phentolamine respectively. The transient phase was of 2-4 min duration and Ca independent whereas the sustained phase of 86Rb release was greatly reduced by the omission of the external Ca. 3. Denervation caused a shift to the left of the 86Rb efflux dose-response curve to carbachol and phenylephrine (3.75- and 3.37-fold respectively). Pilocarpine had similar action to carbachol but it behaved as a partial agonist. 4. Parasympathectomy (Px) increased the intrinsic activity of the partial agonist pilocarpine and converted it to a full agonist. Results of the present study indicate the possibility of an altered receptor-signal transduction mechanism between the receptor and phospholipid turnover/Ca mobilization in the denervated rat parotid gland.
authors.affiliation: Department of Physiology and Pharmacology, University of Southampton, England.
authors.forename: N
authors.initials: N
authors.lastname: Adham
authors.forename: D
authors.initials: D
authors.lastname: Templeton
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D003714:Denervation; D066298:In Vitro Techniques; D008297:Male; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism*; D010646:Phentolamine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D010862:Pilocarpine / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011868:Radioisotopes; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D012413:Rubidium / Q000378:metabolism
chemical_list: D011868:Radioisotopes; D011942:Receptors, Adrenergic, alpha; D010862:Pilocarpine; D010656:Phenylephrine; D000001:Calcimycin; D001285:Atropine; D002217:Carbachol; D012413:Rubidium; D011188:Potassium; D010646:Phentolamine