Electron spin resonance spectroscopy in the study of lymphoid cell receptors.
ESR spectroscopy is a sensitive method which can be used to monitor a wide range of changes in and near the plasma membranes of lymphoid cells during such events as ligand-induced receptor patching and capping, activation and endocytosis and phagocytosis. The advantages of the technique are rapidity, sensitivity, small sample size (10(6)-10(7) cells), and nondestructive nature. Spin labels that are attached to a range of intrinsic and extrinsic molecules give information about the fluidity and polarity of the environment in which they are located. Because of the occurrence of interaction when probes are sequestered in restricted regions of the membrane, ESR spectroscopy is also a valuable technique for measuring the formation of domains in the cell membrane.
Curtain
C C
CC
Looney
F D
FD
Gordon
L M
LM
D002462:Cell Membrane / Q000276:immunology; D003208:Concanavalin A; D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008024:Ligands; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D011971:Receptors, Immunologic / Q000032:analysis*; D013113:Spin Labels
D008024:Ligands; D011971:Receptors, Immunologic; D013113:Spin Labels; D003208:Concanavalin A