[Mutations of plasmid pBS286 blocking the initial stages of naphthalene oxidation induced by Tn5].
A collection of Tn5 transposon Nah- mutants of the plasmid pBS286 was obtained. The insertion sites were localized and orientation of Tn5 determined. The mutants obtained were biochemically analyzed, the nah-region map of the plasmid being elaborated. Structural genes of the nah operon were shown to be organized similarly to those of the nah1 operon of the NAH7 plasmid discussed in the literature. The data obtained are in favour of the previously published information on the presence of elements operating the pBS286 plasmid. The results are given indicating a possibility of regulating the expression of catechol splitting meta-pathway genes with participation of products on early stages of naphthalene oxidation.
Kosheleva
I A
IA
Tsoĭ
T V
TV
Ivashina
T V
TV
Selifonov
S A
SA
Starovoĭtov
I I
II
D001673:Biodegradation, Environmental; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D005796:Genes; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D009281:Naphthalenes / Q000378:metabolism*; D009876:Operon; D010084:Oxidation-Reduction; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics
D004251:DNA Transposable Elements; D009281:Naphthalenes; C031721:naphthalene
Mutatsii plazmidy pBS286, blokiruiushchie pervichnye étapy okisleniia naftalina, indutsirovannye Tn5.