A new approach in recognition of heterochromatic regions of human chromosomes by means of restriction endonucleases.
The heteromorphisms of C-band regions of human chromosomes are evaluated by means of restriction endonucleases AluI, DdeI, MboI, and RsaI. Every chromosome exhibits heteromorphic markers of the C-band regions except chromosome 8. Each enzyme was found to be highly characteristic in its staining profile, a result that clearly suggests the diversity of heterochromatin. The inherent C-band-region heterochromatin variability that is revealed by these enzymes provides a valuable tool in identifying markers as compared with other previously described techniques.
Babu
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Division of Medical Genetics, Beth Israel Medical Center, Mount Sinai School of Medicine, New York.
Agarwal
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AK
Verma
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D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D004262:DNA Restriction Enzymes; D006570:Heterochromatin / Q000235:genetics* / Q000648:ultrastructure; D006801:Humans; D007621:Karyotyping
D006570:Heterochromatin; D004262:DNA Restriction Enzymes