title: Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium.
abstract: We determined the complete nucleotide sequence of the 6354-base-pair plasmid pHV2 of the archaebacterium Halobacterium volcanii. This plasmid is present in approximately six copies per chromosome. We have generated a strain, H. volcanii WFD11, cured of pHV2 by treatment of liquid cultures with ethidium bromide. We describe PEG-mediated transformation of H. volcanii WFD11 with intact pHV2 and with a form of pHV2 marked by a 93-base-pair deletion generated in vitro.
authors.affiliation: Department of Biochemistry, Dalhousie University, Halifax, NS, Canada.
authors.forename: R L
authors.initials: RL
authors.lastname: Charlebois
authors.forename: W L
authors.initials: WL
authors.lastname: Lam
authors.forename: S W
authors.initials: SW
authors.lastname: Cline
authors.forename: W F
authors.initials: WF
authors.lastname: Doolittle
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006217:Halobacterium / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes