title: Biological characterization of human bone tumors. X. The proliferation behavior of macrophages as compared to fibroblastic cells in malignant fibrous histiocytoma and giant cell tumor of bone.
abstract: Seven giant cell tumors of bone and four malignant fibrous histiocytomas were studied immunohistochemically with different monoclonal antibodies to the mononuclear phagocyte system (MPS), to HLA-DR antigens, and to a proliferation-associated nuclear antigen (KI-67), in order to clarify the role of macrophages in these tumors. A part of the mononuclear cells stained positive with antibodies against the MPS. Antibody 25-F-9 against mature tissue macrophages showed the strongest reaction. The osteoclast-like giant cells also stained positive with this antibody. Fibroblast-like stromal cells, however, showed negative reactions to all antibodies against MPS cells. A double-labeling immunohistological technique was used to detect the proliferating cell population in these tumors. The fibroblast-like cells that were negative for MPS markers, were positively labeled with the monoclonal antibody Ki-67 against a proliferation-associated nuclear antigen, whereas a negative reaction to Ki-67 was seen in cells positive with antibodies to the MPS. These results support the concept that macrophages are a reactive population in these tumors, whereas the fibroblast-like mesenchymal cells are the proliferating tumor cells.
authors.affiliation: Gerhard-Domagk-Institut für Pathologie, Westfälische Wilhelms-Universität Münster, Federal Republic of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Roessner
authors.forename: J
authors.initials: J
authors.lastname: Vassallo
authors.forename: E
authors.initials: E
authors.lastname: Vollmer
authors.forename: G
authors.initials: G
authors.lastname: Zwadlo
authors.forename: C
authors.initials: C
authors.lastname: Sorg
authors.forename: E
authors.initials: E
authors.lastname: Grundmann
mesh_terms: D000911:Antibodies, Monoclonal; D001859:Bone Neoplasms / Q000473:pathology* / Q000503:physiopathology; D002455:Cell Division; D005347:Fibroblasts / Q000473:pathology*; D005870:Giant Cell Tumors / Q000473:pathology*; D018219:Histiocytoma, Benign Fibrous / Q000473:pathology*; D007150:Immunohistochemistry; D008264:Macrophages / Q000473:pathology*; D009687:Nuclear Proteins / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D009687:Nuclear Proteins