title: Pentylenetetrazole-induced changes of the single potassium channel in primary cultured cerebral cortical neurons.
abstract: To elucidate the behavior of the single ionic channels of cerebral cortical neurons during bursting activity, the effects of pentylenetetrazole (PTZ) on a single potassium channel of primary cultured cerebral cortical neurons from mice were examined. All of the examined 10-day-old primary cultured neurons of the cerebral cortex showed clear bursting activity after extracellular application of PTZ using whole-cell patch-clamp recording. Less than half of the examined single potassium channels, both outward and inward, of the 2- and 3-day-old as well as 6-10-day-old primary cultured cerebral cortical neurons showed the bursting-type open-close state by application of PTZ. The PTZ-sensitive single potassium channels were found in the voltage-dependent as well as calcium-activated channels.
authors.affiliation: Department of Physiology, Kanagawa Dental College, Yokosuka, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Sugaya
authors.forename: A
authors.initials: A
authors.lastname: Sugaya
authors.forename: T
authors.initials: T
authors.lastname: Takagi
authors.forename: T
authors.initials: T
authors.lastname: Tsuda
authors.forename: K
authors.initials: K
authors.lastname: Kajiwara
authors.forename: K
authors.initials: K
authors.lastname: Yasuda
authors.forename: J
authors.initials: J
authors.lastname: Komatsubara
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000502:physiology*; D004553:Electric Conductivity; D004622:Embryo, Mammalian; D005071:Evoked Potentials / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010433:Pentylenetetrazole / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*
chemical_list: D015221:Potassium Channels; D010433:Pentylenetetrazole