A novel human macrophage-activating factor: distinction from interferon-gamma (IFN-gamma) and granulocyte-macrophage colony-stimulating factor (GMCSF).
Culture supernatant from a human T-cell leukemia virus type I (HTLV-1)-infected cell line, DGA-1, contained a novel macrophage-activating factor (MAF). This MAF was antigenically and functionally distinct from interferon-gamma (IFN-gamma) and from granulocyte-monocyte colony-stimulating factor (GMCSF). Potential contaminants such as bacterial lipopolysaccharide (LPS), Mycoplasma spp, and HTLV-1 were not responsible for this MAF activity. The DGA-1 MAF was secreted constitutively and the cell line grew well in the absence of growth factors such as interleukin-2, mitogen, or antigen. This cell line should provide a good source of this MAF for further purification and characterization.
Jones
M P
MP
Department of Medical Microbiology, London Hospital Medical College, England.
Gunapala
D E
DE
Matutes
E
E
Catovsky
D
D
Coates
A R
AR
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D017977:Deltaretrovirus / Q000502:physiology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D008222:Lymphokines; D008262:Macrophage Activation; D051379:Mice; D013045:Species Specificity; D013601:T-Lymphocytes / Q000502:physiology*
D000943:Antigens, Differentiation; D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D008222:Lymphokines; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006861:Hydrogen Peroxide