Isolation and characterization of the porcine nuclear factor I (NFI) gene.
This study describes the isolation of a major portion of the gene for nuclear factor I (NFI) including its 5'-flanking region with transcriptional start sites. We screened a porcine liver, genomic DNA library in phage EMBL3A with synthetic oligonucleotides derived from tryptic and cyanogen-bromide peptide sequences obtained from purified NFI protein. The NFI gene is present as a single copy in porcine DNA.
Meisterernst
M
M
Institut für Biochemie der Universität München, FRG.
Rogge
L
L
Donath
C
C
Gander
I
I
Lottspeich
F
F
Mertz
R
R
Dobner
T
T
Föckler
R
R
Stelzer
G
G
Winnacker
E L
EL
D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D004720:Endonucleases; D005091:Exons; D005796:Genes; D007438:Introns; D008099:Liver / Q000032:analysis; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009693:Nucleic Acid Hybridization; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases