Anomalous binding of DPDPE as a result of batch variability.
The binding of the delta selective agonist [3H]DPDPE to diencephalic rat brain tissue was examined. Although most material examined gave rise to a single receptor model, one commercial preparation (Amersham, batch 8) suggested a two receptor model. Purification by HPLC removed a minor component and restored a single receptor model to the major component.
Taylor
L
L
Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor 48109-0720.
Mansour
A
A
Watson
S J
SJ
Akil
H
H
D000818:Animals; D001921:Brain / Q000378:metabolism*; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000302:isolation & purification / Q000378:metabolism* / Q000592:standards; D011786:Quality Control; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism*; D017465:Receptors, Opioid, delta
D004745:Enkephalins; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D020881:Enkephalin, D-Penicillamine (2,5)-