title: Influence of organ microenvironment on pigmentation of a metastatic murine melanoma.
abstract: The purpose of these studies was to investigate the relationship of the host microenvironment to the metastatic and pigmented phenotypes of the SW-1 variant of the murine K-1735 melanoma. The SW-1 subline was isolated from an amelanotic lung metastasis in a C3H/HeN mouse given an s.c. injection of the K-1735 melanoma. Cells of this line were highly metastatic and produced tumor deposits in many organs. In all sites except the brain, these lesions were predominantly amelanotic. K-1735 SW-1 cells were isolated from metastases in various organs and subsequently reinoculated into normal syngeneic recipients. Whereas the metastatic phenotype remained stable and thus was heritable, pigmentation was unstable and appeared to be modulated by the site of tumor growth. Further differences in the phenotype of K-1735 SW-1 cells growing in vivo and in culture were revealed by assays for tyrosinase activity. K-1735 SW-1 cells growing in culture did not produce melanin nor did they respond to agents that can stimulate melanin production in another mouse melanoma, the B16 line. K-1735 SW-1 cells do not, however, lack tyrosinase, since these cells are capable of producing melanin when growing in certain organs in vivo. We conclude that the host organ environment may influence a phenotype of malignant melanoma cells, i.e., pigmentation. These findings also suggest caution when extrapolating the results of in vitro biochemical assays to properties of tumor cells growing in vivo.
authors.affiliation: Department of Cell Biology, University of Texas M.D. Anderson Hospital and Tumor Institute at Houston 77030.
authors.forename: J E
authors.initials: JE
authors.lastname: Price
authors.forename: D
authors.initials: D
authors.lastname: Tarin
authors.forename: I J
authors.initials: IJ
authors.lastname: Fidler
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis; D008546:Melanoma, Experimental / Q000503:physiopathology* / Q000648:ultrastructure; D051379:Mice; D008809:Mice, Inbred C3H; D008854:Microscopy, Electron; D014442:Monophenol Monooxygenase / Q000032:analysis; D009362:Neoplasm Metastasis; D009928:Organ Specificity; D010858:Pigmentation; D014407:Tumor Cells, Cultured
chemical_list: D008543:Melanins; D000242:Cyclic AMP; D014442:Monophenol Monooxygenase