Irreproducibility of neutralization of herpes simplex virus under conditions where antibody is not in excess.
Neutralizing activity against herpes simplex virus was significantly reduced if initial virus titers were greater than 10(6) PFU/ml; there was no significant neutralization when initial virus titers approached 10(8) PFU/ml. This was a result of utilization of all available antibody by virus particles and 'free' virus antigen and emphasizes the importance of conducting virus neutralization tests under conditions of antibody excess.
Shariff
D
D
Department of Medical Microbiology, University of Birmingham, UK.
Hallworth
J A
JA
Buchan
A
A
Skinner
G R
GR
D000914:Antibodies, Viral / Q000008:administration & dosage; D000956:Antigens, Viral / Q000008:administration & dosage; D009500:Neutralization Tests / Q000379:methods*; D018139:Simplexvirus / Q000145:classification / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral