title: Subpopulation of alveolar macrophages inhibits superoxide anion production by macrophages.
abstract: Pig alveolar macrophages are a heterogeneous population of cells. Three subpopulations or bands exist when the whole population is separated according to density. Band 1 cells are the least dense cells and constitute 9% of the total population. Bands 2 and 3 represent 44 and 47% of the total population. The three subpopulations generate superoxide anions, although to varying degrees. Band 3 cells are the most active, while band 1 cells are the least active. The amount of superoxide anions released in a mixed population of bands 1, 2, and 3 cells was less than the sum of that produced from each band assayed separately. Band 1 cells were found to inhibit by 47% the production of superoxide anions by band 3 cells. Conditioned medium from band 1 cells contains a heat-sensitive, nondialyzable, soluble factor responsible for this inhibition.
authors.affiliation: Department of Anesthesiology, School of Medicine, University of Missouri-Columbia 65212.
authors.forename: R B
authors.initials: RB
authors.lastname: Zeidler
authors.forename: J A
authors.initials: JA
authors.lastname: Flynn
authors.forename: J C
authors.initials: JC
authors.lastname: Arnold
authors.forename: N S
authors.initials: NS
authors.lastname: Conley
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D002450:Cell Communication; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D008262:Macrophage Activation; D008264:Macrophages / Q000145:classification / Q000378:metabolism / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000166:cytology; D013481:Superoxides / Q000378:metabolism*; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate