title: Organisation of the regulatory region of the Escherichia coli melibiose operon.
abstract: The regulatory region of the Escherichia coli melibiose operon contains two divergent promoters. One promoter is responsible for the expression of the melR gene, that is essential for melibiose-dependent stimulation of the second promoter. Melibiose-induced transcription from this second promoter initiates at a start point 25 bp upstream from the start codon of the melA gene, encoding an alpha-galactosidase. The nucleotide sequence covering the divergent promoters and the melR gene is reported.
authors.affiliation: Department of Biochemistry, University of Birmingham, U.K.
authors.forename: C
authors.initials: C
authors.lastname: Webster
authors.forename: K
authors.initials: K
authors.lastname: Kempsell
authors.forename: I
authors.initials: I
authors.lastname: Booth
authors.forename: S
authors.initials: S
authors.lastname: Busby
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004187:Disaccharides / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008553:Melibiose / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004187:Disaccharides; D008553:Melibiose; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase