title: Small increases in pH decrease uptake of Escherichia coli by human neutrophils in vitro.
abstract: Increases in pH from 7.4 to 7.8 decreased the ability of human neutrophils in serum to ingest and kill Escherichia coli ATCC 29552 in vitro. In contrast, similar increases in pH did not decrease the bactericidal activity of neutrophils in serum against Staphylococcus aureus 502A. Increases in pH did not alter opsonization of E. coli by serum or the growth of E. coli but rather appeared to alter neutrophil uptake of bacteria by a direct effect on the neutrophil.
authors.affiliation: Department of Medicine, Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver.
authors.forename: E M
authors.initials: EM
authors.lastname: Berger
authors.forename: K
authors.initials: K
authors.lastname: Murphy
authors.forename: G A
authors.initials: GA
authors.lastname: Shibao
authors.forename: J E
authors.initials: JE
authors.lastname: Repine
mesh_terms: D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000276:immunology; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides