Vimentin, a cytoskeletal substrate of protein kinase C.
Evidence is shown that vimentin, the intermediate filament protein, is a substrate for protein kinase C: (a) Purified vimentin from Chinese hamster ovary cells can be phosphorylated by protein kinase C prepared from rabbit peritoneal neutrophils. Tryptic peptic analysis reveals multiple sites of phosphorylation distinct from those phosphorylated by cAMP-dependent protein kinase. (b) phosphorylation of membrane associated vimentin is stimulated in phorbol 12-myristate 13-acetate treated neutrophil membranes, suggesting that vimentin can be a substrate for membrane associated protein kinase C and (c) phorbol 12-myristate 13-acetate also stimulates the phosphorylation of vimentin in 32P-labeled intact neutrophils.
Huang
C K
CK
Department of Pathology, University of Connecticut Health Center, Farmington 06032.
Devanney
J F
JF
Kennedy
S P
SP
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002467:Cell Nucleus / Q000201:enzymology; D006224:Cricetinae; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D009504:Neutrophils / Q000201:enzymology; D010053:Ovary / Q000032:analysis; D010446:Peptide Fragments / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D011817:Rabbits; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism*
D010446:Peptide Fragments; D014746:Vimentin; D000242:Cyclic AMP; D011494:Protein Kinases; D011493:Protein Kinase C; D014357:Trypsin; D013755:Tetradecanoylphorbol Acetate