Receptors on phaeochromocytoma cells for two members of the PP-fold family--NPY and PP.
Pancreatic polypeptide (PP) and neuropeptide Y (NPY) belong to a family of regulatory peptides which hold a distinct tertiary structure, the PP-fold, even in dilute aqueous solution. High-affinity receptors, specific for both PP and NPY, are described on the rat phaeochromocytoma cell line, PC-12. The binding of [125I-Tyr36]PP to PC-12 cells was inhibited by concentrations of unlabeled PP which correspond to physiological concentrations of the hormone, 10(-11)-10(-9) mol/l. The affinity of the receptor for the neuropeptide, NPY, was 10(2)-times lower than that of the PP receptor. C-terminal fragments of both PP (PP24-36) and NPY (NPY13-36) were between 10(2)- and 10(3)-times less potent in displacing the radiolabeled 36-amino-acid peptides from their respective receptors. It is concluded that PC-12 cells are suited for structure-function studies of the PP-fold peptides and studies on the cellular events following cellular binding of PP-fold peptides.
Schwartz
T W
TW
Laboratory for Molecular Endocrinology, University Department of Clinical Chemistry, Rigshopitalet, Denmark.
Sheikh
S P
SP
O'Hare
M M
MM
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D007457:Iodine Radioisotopes; D007700:Kinetics; D007470:Monoiodotyrosine; D009478:Neuropeptide Y / Q000378:metabolism; D010191:Pancreatic Polypeptide / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism*; D051381:Rats; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism*; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D007457:Iodine Radioisotopes; D009478:Neuropeptide Y; D010446:Peptide Fragments; D011964:Receptors, Gastrointestinal Hormone; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter; C029438:receptors, pancreatic polypeptide-specific; D010191:Pancreatic Polypeptide; D007470:Monoiodotyrosine