Human liquid bone marrow culture in serum-free medium.
Prolonged in vitro maintenance of human bone marrow progenitor cells was achieved using a serum-free (SF) liquid culture system. Culture medium was based on Iscove's medium supplemented with bovine serum albumin, human transferrin, bovine insulin, soybean lecithin, cholesterol, hydrocortisone and alpha-thioglycerol. Under these standardized culture conditions, CFU-GM were maintained for up to 4 weeks, as is the case when using conventional serum-dependent medium. Erythropoiesis exhibited a slower decline than that found using serum containing medium. Development of normal haematopoiesis was effective in spite of poor stromal cell development--a confluent adherent layer as classically described in serum conditions was never achieved. Our newly defined system provides a reliable technique for studying human haematopoietic stem cell proliferation and differentiation in vitro; it allows for rational utilization of currently available purified recombinant growth factors. It may be a promising tool in the clinical use of cultured haematopoietic stem cells.
Drouet
X
X
Institut National de Transfusion Sanguine, Paris, France.
Douay
L
L
Giarratana
M C
MC
Baillou
C
C
Gorin
N C
NC
Salmon
C
C
Najman
A
A
D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D013997:Time Factors
D003470:Culture Media