Evolution of P transposable elements: sequences of Drosophila nebulosa P elements.
P elements have been cloned and sequenced from Drosophila nebulosa. Their sequences have diverged less than 6% from P elements of Drosophila melanogaster. However D. nebulosa P elements have nucleotide changes that close all four open reading frames found in the D. melanogaster P element. Microinjection experiments show that D. nebulosa P elements cannot provide transposase function for D. melanogaster P elements, nor are D. nebulosa P elements mobilized by the transposase provided by a D. melanogaster P factor. Three D. nebulosa P elements appear to have integrated into the same position of a complex, centromeric repeated sequence. Comparison of nucleotide sequences suggests that D. nebulosa P elements have diverged upon different pathways from a common ancestor that was 99% homologous to the P elements of D. melanogaster.
Lansman
R A
RA
Shade
R O
RO
Grigliatti
T A
TA
Brock
H W
HW
D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics; D004463:Ecology; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes