Characterization and mapping of equine herpesvirus type 1 immediate early, early, and late transcripts.
Northern blot analysis was used to characterize and map equine herpesvirus type 1 (EHV-1) immediate early (IE), early, and late transcripts. Genomic EHV-1 DNA and cloned EHV-1 restriction endonuclease fragments, representing the entire genome, were 32P-labeled and hybridized to immobilized total cell RNA isolated from EHV-1 infected rabbit kidney cells incubated in the presence or absence of metabolic inhibitors. A single 6.0 kilobase (kb) IE transcript mapped to viral inverted repeat sequences. Approximately 41-45 early transcripts ranging in size from 0.8 to 6.4 kb and 18-20 late transcripts ranging in size from 0.8 to 10.0 kb were identified. These findings demonstrate that EHV-1 gene expression is regulated at the level of transcription, although regulation at the level of translation is also possible. The results provide a basis for examining alterations in viral gene expression in EHV-1 oncogenically transformed and persistently infected cells.
Gray
W L
WL
Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130.
Baumann
R P
RP
Robertson
A T
AT
O'Callaghan
D J
DJ
Staczek
J
J
D000818:Animals; D002874:Chromosome Mapping; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral; D004262:DNA Restriction Enzymes