Characterization and cloning of fasciclin I and fasciclin II glycoproteins in the grasshopper.
Monoclonal antibodies were previously used to identify two glycoproteins, called fasciclin I and II (70 and 95 kDa, respectively), which are expressed on different subsets of axon fascicles in the grasshopper (Schistocerca americana) embryo. Here the monoclonal antibodies were used to purify these two membrane-associated glycoproteins for further characterization. Fasciclin II appears to be an integral membrane protein, whereas fasciclin I is an extrinsic membrane protein. The amino acid sequences of the amino terminus and fragments of both proteins were determined. Using synthetic oligonucleotide probes and antibody screening, we isolated genomic and cDNA clones. Partial DNA sequences of these clones indicate that they encode fasciclins I and II.
Snow
P M
PM
Department of Biological Sciences, Stanford University, CA 94305.
Zinn
K
K
Harrelson
A L
AL
McAllister
L
L
Schilling
J
J
Bastiani
M J
MJ
Makk
G
G
Goodman
C S
CS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015816:Cell Adhesion Molecules, Neuronal; D002462:Cell Membrane / Q000032:analysis; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D006110:Grasshoppers / Q000032:analysis*; D007163:Immunosorbent Techniques; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009420:Nervous System / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments; D012333:RNA, Messenger / Q000235:genetics; D014357:Trypsin
D015816:Cell Adhesion Molecules, Neuronal; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D012333:RNA, Messenger; C051641:fasciclin I; C051642:fasciclin II; D004247:DNA; D004262:DNA Restriction Enzymes; D014357:Trypsin