title: Subunit III of cytochrome c oxidase is expressed in Paracoccus denitrificans.
abstract: Polyclonal antibodies have been obtained against a synthetic dodecapeptide identical to the aminoacid sequence 120-131 DSPIKDGVWPPE (inferred from its DNA sequence) of Paracoccus denitrificans cytochrome c oxidase subunit III. The antibodies had a titer higher than 1:10000 when tested against the antigen. These antibodies have been used to produce immunological evidence that, despite the fact that subunit III is not isolated with cytochrome c oxidase, it exists in Paracoccus denitrificans lysates. The antibodies did not show reactivity with bovine heart cytochrome c oxidase either by ELISA or immunoblotting. It was also shown that the antibodies react with a single polypeptide present in Paracoccus denitrificans cell lysates, having an apparent molecular weight close to that of subunit III of bovine heart oxidase.
authors.affiliation: Institut für Biochemie und Molekularbiologie der Universität Bern, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: N
authors.initials: N
authors.lastname: Labonia
authors.forename: A
authors.initials: A
authors.lastname: Azzi
mesh_terms: D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000032:analysis; D002417:Cattle; D003576:Electron Transport Complex IV / Q000235:genetics*; D005796:Genes; D046911:Macromolecular Substances; D010231:Paracoccus denitrificans / Q000201:enzymology / Q000235:genetics*; D011487:Protein Conformation
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D046911:Macromolecular Substances; D003576:Electron Transport Complex IV