Intranasal immunization of hamsters with envelope glycoproteins of human parainfluenza virus type 3.
Envelope glycoproteins of human parainfluenza virus type 3 (PIV-3) were selectively solubilized with n-octyl beta-D-glucopyranoside and reconstituted into lipid vesicles by dialysis of the detergent. The efficacy of the glycoprotein preparation as a subunit vaccine when administered to hamsters intranasally or subcutaneously was compared. Animals receiving four intranasal immunizations with 5 micrograms of the glycoprotein preparation were completely resistant to challenge infection. Only partial protection, however, was observed in animals immunized subcutaneously with the same dose of antigen. The local glycoprotein-specific IgA response was significantly higher in intranasally immunized animals and was implicated in resistance to challenge infection.
Ray
R
R
Molecular Engineering Associates, Inc., Birmingham, Alabama 35203.
Glaze
B J
BJ
Moldoveanu
Z
Z
Compans
R W
RW
D000281:Administration, Intranasal; D000818:Animals; D000956:Antigens, Viral / Q000008:administration & dosage; D006224:Cricetinae; D003902:Detergents; D007114:Immunization; D007070:Immunoglobulin A / Q000276:immunology*; D007279:Injections, Subcutaneous; D008970:Molecular Weight; D009500:Neutralization Tests; D010224:Parainfluenza Virus 3, Human / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000517:prevention & control*; D018108:Respirovirus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000956:Antigens, Viral; D003902:Detergents; D007070:Immunoglobulin A; D014759:Viral Envelope Proteins