5-Hydroxytryptamine stimulates the formation of inositol phosphate in astrocytes from different regions of the brain.
5-Hydroxytryptamine (5-HT) stimulated the turnover of phosphoinositide in primary cultures of astroglia from the cerebral cortex, striatum, hippocampus and brain stem. Ketanserin and ritanserin, selective antagonists for the central 5-HT2 receptor, inhibited the 5-HT-stimulated formation of inositol monophosphate. In contrast, there was no statistically significant accumulation of cyclic AMP after incubation with different concentrations of 5-HT in any of the cultures studied. The results indicate that astrocytes from various regions of the brain possess 5-HT2 receptors coupled to the formation of inositol phosphates.
Hansson
E
E
Institute of Neurobiology, University of Göteborg, Sweden.
Simonsson
P
P
Alling
C
C
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis; D007295:Inositol Phosphates / Q000096:biosynthesis*; D007650:Ketanserin / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D016713:Ritanserin; D012701:Serotonin / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013403:Sugar Phosphates / Q000096:biosynthesis*; D014018:Tissue Distribution
D007295:Inositol Phosphates; D010880:Piperidines; D013403:Sugar Phosphates; D016713:Ritanserin; D012701:Serotonin; D007650:Ketanserin; D000242:Cyclic AMP