Transferable amikacin resistance in Acinetobacter spp. due to a new type of 3'-aminoglycoside phosphotransferase.
Acinetobacter baumannii BM2580 resistant to kanamycin and structurally related antibiotics, including amikacin, was isolated from a clinical specimen. A phosphocellulose paper-binding assay and DNA annealing studies indicated that resistance to aminoglycosides in BM2580 was due to synthesis of a new type of 3'-aminoglycoside phosphotransferase. The gene conferring resistance to kanamycin-amikacin in this strain was carried by a 63-kilobase plasmid, pIP1841, self-transferable to A. baumannii, A. haemolyticus, and A. lwoffii but not to Escherichia coli. The aminoglycoside resistance gene of pIP1841 was cloned in E. coli, where it was expressed.
Lambert
T
T
Hôpital Saint-Michel, Paris, France.
Gerbaud
G
G
Courvalin
P
P
D000150:Acinetobacter / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D000583:Amikacin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006801:Humans; D019868:Kanamycin Kinase; D009693:Nucleic Acid Hybridization; D010770:Phosphotransferases / Q000096:biosynthesis* / Q000235:genetics; D011815:R Factors
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D000583:Amikacin; D010770:Phosphotransferases; D019868:Kanamycin Kinase