Comparison of Track XI fluorometric immunoassay with Bio-EnzaBead enzyme-linked immunosorbent assay for detection of serum antibody to mouse hepatitis virus.
The Track XI system (Microbiological Associates, Bethesda, Md.) was compared with the Bio-EnzaBead assay (Organon Teknika, Durham, N.C.) for the detection of antibody to mouse hepatitis virus (MHV). Strain A/J mice were inoculated intranasally with MHV type 3. Sera were collected at 1, 2, 4, and 9 weeks postinoculation and tested. Individual serum samples were retested twice by each method. The results suggested that the Track XI system was more sensitive and reliable than the Bio-EnzaBead assay in detecting antibody to MHV type 3 in individual serum samples from A/J mice.
La Regina
M C
MC
Department of Comparative Medicine, St. Louis University Medical School, Missouri 63104.
Lonigro
J
J
Woods
L
L
Hall
W C
WC
Doyle
R E
RE
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006524:Hepatitis, Viral, Animal / Q000276:immunology*; D051379:Mice; D006517:Murine hepatitis virus / Q000276:immunology*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic