Rotavirus-specific cytotoxic T lymphocytes cross-react with target cells infected with different rotavirus serotypes.
Splenocytes from adult C57BL6 (H-2b) mice orally inoculated with nonmurine rotaviruses lysed syngeneic rotavirus-infected target cells. Cytotoxic T lymphocytes (CTLs) were responsible for this cytotoxic activity. Cytotoxic activity was (i) detected 7 days after primary oral inoculation; (ii) not detected in uninoculated animals; (iii) specific for rotavirus-infected target cells; (iv) eliminated by treatment with Thy 1.2-specific immunoglobulin M and complement; and (v) restricted at H-2Db. In addition, rotavirus-specific CTLs cross-reacted with target cells infected with different human or animal rotavirus serotypes. Heterotypic protection against rotavirus challenge may be mediated by cross-reactive CTLs.
Offit
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Department of Medical Microbiology, Stanford University School of Medicine, California 94305.
Dudzik
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D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D007156:Immunologic Memory; D051379:Mice; D012401:Rotavirus / Q000145:classification / Q000276:immunology*; D012703:Serotyping; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte