Nerve growth factor transiently increases tetrahydrobiopterin and total biopterin contents of pheochromocytoma PC12h cells.
Nerve growth factor (NGF) is known to induce differentiation of pheochromocytoma into sympathetic neuron-like cells. Tetrahydrobiopterin (BPH4) and total biopterin (BP) levels in PC12h, a subclonal line of PC12, were transiently increased by NGF: the increase in BPH4 and BP reached the maximum (20-25 ng/mg protein = about 2-fold over the control level) at 24 h after the treatment was started. After 2-3 days, the BPH4 and BP levels decreased to the same level as in control cells. The NGF concentration which gave a half maximal BP increase by 24 h-treatment was around 1 ng/ml.
Suzuki
H
H
Department of Pedodontics, Josai Dental University, Saitama, Japan.
Nakanishi
N
N
Yamada
S
S
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D001708:Biopterins / Q000031:analogs & derivatives* / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000378:metabolism*; D051381:Rats
D009414:Nerve Growth Factors; D001708:Biopterins; D003994:Bucladesine; C003402:sapropterin