Detection of biospecific interactions using amplified ellipsometry.
Amplified detection of biomolecules and biological interactions using an optical surface technique, ellipsometry, is demonstrated for two biosystems--immunoglobulin G with anti-immunoglobulin G (IgG) and the lectin concanavalin A (Con A) with yeast cells. In order to improve the sensitivity of the ellipsometer signal, an amplifier conjugate is formed by binding the affinity ligand to a 12-nm silica particle which is readily detected by the ellipsometer. Thus by using conjugates of IgG-silica and Con A-silica, amplifications of five to seven times have been obtained enabling detection of less than 20 pg/mm2 of biomolecular material.
Mandenius
C F
CF
Department of Pure and Applied Biochemistry, University of Lund, Sweden.
Mosbach
K
K
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology; D001666:Binding Sites, Antibody; D003208:Concanavalin A / Q000494:pharmacology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D055095:Optics and Photonics; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D012822:Silicon Dioxide
D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D007074:Immunoglobulin G; D003208:Concanavalin A; D012822:Silicon Dioxide