title: Restriction map of a capsule plasmid of Bacillus anthracis.
abstract: The capsule plasmid pTE702 of Bacillus anthracis has been physically mapped with the restriction endonucleases HindIII, PstI, BamHI, SalI, and XhoI. A HindIII fragment map of pTE702 (96.5 kb) was obtained by analysis of the recombinant plasmids and cosmids containing overlapping fragments partially digested with HindIII. The physical map for PstI, BamHI, SalI, and XhoI was obtained by double digestion mapping of these sites in relation to the HindIII sites. The replication region of pTE702 was determined by in vitro genetic replicon labeling in B. subtilis.
authors.affiliation: National Institute of Animal Health, Tsukuba Science City, Ibaraki, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Uchida
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: S
authors.initials: S
authors.lastname: Makino
authors.forename: C
authors.initials: C
authors.lastname: Sasakawa
authors.forename: M
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: N
authors.initials: N
authors.lastname: Terakado
mesh_terms: D001408:Bacillus anthracis / Q000235:genetics* / Q000472:pathogenicity; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D009710:Nucleotide Mapping; D010957:Plasmids; D014774:Virulence
chemical_list: D004262:DNA Restriction Enzymes