On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision.
The rate of GTP hydrolysis in the active site of transducin and that of the release of the phosphate thus formed have been measured. The former step has been found to be a rate-limiting one. The rate constant for GTP hydrolysis is equal to 0.027 s-1 at 23 degrees C, and 0.07 s-1 at 37 degrees C. Besides, it has been shown that the rate of GTPase reaction on the transducin alpha-subunit does not depend on the concentration of a complex of transducin beta- and gamma-subunits or on the presence of cGMP phosphodiesterase and a 48 kDa protein from rod outer segments. According to the results, GTP hydrolysis on transducin proceeds too slowly to account for the rapid quenching of a phosphodiesterase cascade in rod outer segments.
Arshavsky VYu
A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Antoch
M P
MP
Philippov
P P
PP
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000502:physiology*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008565:Membrane Proteins / Q000502:physiology*; D010744:Phosphoric Monoester Hydrolases / Q000502:physiology*; D010766:Phosphorylation; D010775:Photic Stimulation; D010786:Photoreceptor Cells / Q000502:physiology; D012243:Rhodopsin / Q000378:metabolism / Q000528:radiation effects; D012374:Rod Cell Outer Segment / Q000502:physiology; D015293:Transducin; D014785:Vision, Ocular / Q000502:physiology*
D008565:Membrane Proteins; D006160:Guanosine Triphosphate; D012243:Rhodopsin; D010744:Phosphoric Monoester Hydrolases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D020558:GTP Phosphohydrolases; D015293:Transducin