title: Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L. An abundant transcript induced by transformation of fibroblasts.
abstract: Transfection of an activated rat oncogene into NIH3T3 fibroblasts leads to transformation and induction of a metastatic phenotype. To identify genes whose activation might mediate these processes, we used a differential screening strategy. A 1.5-kb transcript is induced fiftyfold, constitutes 1% of ras transformed cell messenger RNA (mRNA) and is the most abundantly induced message in these cells. Our sequence data shows that it encodes murine cathepsin L, a potent collagenolytic and elastinolytic lysosomal enzyme. The murine clone was used to isolate human cathepsin L complementary DNA (cDNA) clones. The complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L are presented and compared to other papain family cysteine proteinases. Northern analysis shows that both human and murine cathepsin L probes hybridize to a 1.5-kb transcript in several tissues, but also to a 4-kb transcript in human kidney. These clones will facilitate studies of the structure, expression, and function of cathepsin L, including its unexpected upregulation in transformation.
authors.affiliation: Howard Hughes Medical Institute, Department of Medicine, University of Chicago, Illinois 60637.
authors.forename: L J
authors.initials: LJ
authors.lastname: Joseph
authors.forename: L C
authors.initials: LC
authors.lastname: Chang
authors.forename: D
authors.initials: D
authors.lastname: Stamenkovich
authors.forename: V P
authors.initials: VP
authors.lastname: Sukhatme
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002403:Cathepsins / Q000235:genetics*; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D004792:Enzyme Precursors / Q000235:genetics*; D005347:Fibroblasts; D011905:Genes, ras; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D004792:Enzyme Precursors; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D002403:Cathepsins; C055808:preprocathepsin L