Growth hormone gene expression in myoepithelial cells directed by various eucaryotic transcriptional regulatory sequences.
Mammary gland myoepithelial cells were isolated from cattle and cell lines were established. Cells were plated onto tissue culture dishes with or without collagen. Cells were transfected with bovine growth hormone rDNA containing one of the following eucaryotic transcriptional regulatory sequences: human cytomegalovirus immediate early promoter, simian virus 40 early promoter, mouse metallothionein I promoter and the Rous sarcoma virus long terminal repeat. These sequences were evaluated for their ability to direct recombinant bovine growth hormone DNA expression in myoepithelial cells. The most effective transcriptional regulatory sequences were the cytomegalovirus immediate early and simian virus 40 early promoters.
Gorewit
R C
RC
Department of Animal Science, Cornell University, Ithaca, NY 14853.
Chen
H Y
HY
Kopchick
J J
JJ
D000818:Animals; D002417:Cattle; D003587:Cytomegalovirus / Q000235:genetics; D004274:DNA, Recombinant; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D013006:Growth Hormone / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008668:Metallothionein / Q000235:genetics; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D004274:DNA, Recombinant; D013006:Growth Hormone; D008668:Metallothionein