title: Nucleotide sequence of the Clostridium acidiurici ("Clostridium acidi-urici") gene for 10-formyltetrahydrofolate synthetase shows extensive amino acid homology with the trifunctional enzyme C1-tetrahydrofolate synthase from Saccharomyces cerevisiae.
abstract: The nucleotide sequence of the gene for 10-formyltetrahydrofolate synthetase (EC 6.3.4.3) from Clostridium acidiurici ("Clostridium acidi-urici") was determined. The synthetase mRNA initiation and termination regions were determined by primer extension and S1 nuclease mapping. Two potential -10 and -35 promoter regions were identified upstream of mRNA initiation. The terminator region was found to be in a large region of dyad symmetry. A comparison of the amino acid sequences of the monofunctional synthetase and the eucaryotic trifunctional enzyme, C1-tetrahydrofolate synthase, from Saccharomyces cerevisiae demonstrated a region of strong homology.
authors.affiliation: Department of Biochemistry, University of California, Berkeley 94720.
authors.forename: T R
authors.initials: TR
authors.lastname: Whitehead
authors.forename: J C
authors.initials: JC
authors.lastname: Rabinowitz
mesh_terms: D000595:Amino Acid Sequence; D000619:Aminohydrolases / Q000235:genetics*; D001483:Base Sequence; D003013:Clostridium / Q000201:enzymology / Q000235:genetics*; D003062:Codon / Q000235:genetics; D004720:Endonucleases; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics*; D005798:Genes, Bacterial; D005800:Genes, Fungal; D008025:Ligases / Q000235:genetics*; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D009097:Multienzyme Complexes; D012333:RNA, Messenger; C021836:formyl-methenyl-methylenetetrahydrofolate synthetase; D010088:Oxidoreductases; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D000619:Aminohydrolases; D008025:Ligases; D005574:Formate-Tetrahydrofolate Ligase