Characterization of cholesterol hemisuccinate and alpha-tocopherol hemisuccinate vesicles.
Cholesterol hemisuccinate (CHS) and alpha-tocopherol hemisuccinate (alpha-THS) were found to be capable of forming liposomes of multi- or single lamellar character. Such vesicles formed spontaneously, did not require the use of organic solvents and yielded high trapping efficiencies and captured volumes. Both CHS and alpha-THS systems greatly restricted the motion of intercalated spin labelled fatty acids, yet were more osmotically responsive than similar vesicle types comprised of phosphatidylcholine. Small angle X-ray diffraction measurements were consistent with vesicles possessing extremely weak interlamellar forces. CHS vesicles were found to remain intact in vivo, yet followed a pattern of distribution dissimilar to phosphatidylcholine vesicles.
Janoff
A S
AS
Liposome Company, Inc., Princeton, NJ 08540.
Kurtz
C L
CL
Jablonski
R L
RL
Minchey
S R
SR
Boni
L T
LT
Gruner
S M
SM
Cullis
P R
PR
Mayer
L D
LD
Hope
M J
MJ
D000818:Animals; D002152:Calorimetry, Differential Scanning; D002788:Cholesterol Esters; D002860:Chromium Radioisotopes; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005614:Freeze Fracturing; D008051:Lipid Bilayers; D008081:Liposomes / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D013113:Spin Labels; D014018:Tissue Distribution; D024505:Tocopherols; D014810:Vitamin E / Q000031:analogs & derivatives*; D014961:X-Ray Diffraction
D002788:Cholesterol Esters; D002860:Chromium Radioisotopes; D003497:Cyclic N-Oxides; D008051:Lipid Bilayers; D008081:Liposomes; D013113:Spin Labels; D014810:Vitamin E; D024505:Tocopherols; C013440:cholesteryl succinate