Identification of Alu transposition in human lung carcinoma cells.
We have demonstrated genetic transposition in human cells. An experimental system was established in which the Ecogpt (gpt) gene was employed as a target for inactivation. The human lung carcinoma cell line A549 containing this target was fused to UV-irradiated A549 cells that did not contain the target. From the fusion products, sublines carrying an inactivated gpt gene were analyzed. UV irradiation increased the frequency of inactivated gpt genes in the fusion cells by 100-fold. One subline was found to contain a complete Alu sequence in the coding region of the gpt gene. The inserted element differed from the Blur8 sequence by only 7 out of the 270 nucleotides. The insertion of this Alu element created a 5 bp insertion site duplication.
Lin
C S
CS
Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Goldthwait
D A
DA
Samols
D
D
D001345:Autoradiography; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010430:Pentosyltransferases / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements; D004247:DNA; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase