title: Allelic variation within the Emv-15 locus defines genomic sequences closely linked to the agouti locus on mouse chromosome 2.
abstract: Gene(s) at the agouti locus act within the microenvironment of the hair follicle to switch pigment synthesis in the melanocyte between eumelanin (black or brown pigment) and phaeomelanin (yellow pigment). Many phenotypic variants of this locus have been described. The mechanism(s) of gene action causing such variation in coat-color phenotype is not known. The close linkage of an endogenous ecotropic murine leukemia provirus, Emv-15, to the lethal yellow mutation of the agouti locus provides a means to molecularly access genes at or near the agouti locus. We have identified and used a unique mouse sequence flanking the Emv-15 provirus to define three alleles of the Emv-15 locus. We found a correlation between the presence of specific Emv-15 alleles and the origins of specific agouti locus mutations, confirming close linkage. However, we found some exceptions which suggest that the Emv-15 locus is closely linked to, but genetically separable from, the agouti locus.
authors.affiliation: Mammalian Genetics Laboratory, NCI-Frederick Cancer Research Facility, Maryland 21701.
authors.forename: L D
authors.initials: LD
authors.lastname: Siracusa
authors.forename: L B
authors.initials: LB
authors.lastname: Russell
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D005796:Genes; D008040:Genetic Linkage; D014644:Genetic Variation; D006200:Hair Color; D008099:Liver / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D013045:Species Specificity; D013154:Spleen / Q000032:analysis
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes