Interaction of S-100b protein with cardiolipin vesicles as monitored by electron spin resonance, pyrene fluorescence and circular dichroism.
The interaction of S-100b protein with cardiolipin (CL) vesicles has been studied by electron spin resonance, pyrene fluorescence, and circular dichroism. Electron spin resonance and pyrene fluorescence data indicate that S-100b binds to the polar surface of vesicles Ca2+-independently. In the presence of Ca2+, S-100b potentiates the Ca2+-induced clustering of the polar headgroups of CL molecules and causes a further reduction in the Ca2+-dependent decrease in the lateral mobility of the pyrene inserted into the lipid bilayer, which points to an effect of the protein on the hydrophobic core of the lipid bilayer through a larger perturbation of its polar surface. Circular dichroism analyses indicate that CL vesicles cause a decrease in the alpha-helical content of S-100b, analogous to that produced by Ca2+ and that the effects of CL vesicles and of Ca2+ on the secondary structure of the protein are supra-additive. By this technique, we found that the affinity of Ca2+ for S-100b increases substantially in the presence of CL vesicles, even in the presence of physiologic concentrations of KCl, suggesting that once S-100b had interacted with CL vesicles it assumes a new conformation in which its Ca2+-binding properties are greatly enhanced. These results are discussed in relation to binding of S-100b proteins to natural membranes, and to a possible involvement of S-100b in the regulation of membrane structural organization.
Zolese
G
G
Institute of Biochemistry, Medical School, Ancona, Italy.
Tangorra
A
A
Curatola
G
G
Giambanco
I
I
Donato
R
R
D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002308:Cardiolipins; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D005453:Fluorescence; D005470:Fluorometry; D008081:Liposomes / Q000378:metabolism*; D009414:Nerve Growth Factors; D011487:Protein Conformation; D011721:Pyrenes; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism*
D002308:Cardiolipins; D008081:Liposomes; D009414:Nerve Growth Factors; D011721:Pyrenes; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D002118:Calcium