Fructose 2,6-bisphosphate in isolated foetal hepatocytes.
Fru 2,6-P2 was present in isolated foetal hepatocytes at a concentration of 1.6 nmol per g cells. When foetal hepatocytes were exposed to glucagon no changes were observed either in the concentration of Fru 2,6-P2 and lactate release or in the activities of 6-phosphofructo-2-kinase and pyruvate kinase. Incubation of purified 6-phosphofructo-2-kinase with the catalytic subunit of protein kinase did not change the enzyme activity. The inhibition by sn-glycerol 3-phosphate was much lower for the foetal than for adult enzyme. These results suggest that an isoenzyme of 6-phosphofructo-2-kinase in foetal hepatocytes different from that of adult hepatocytes may be present.
Martín-Sanz
P
P
Instituto de Bioquímica, Centro Mixto del CSIC, Facultad de Farmacia,Universidad Complutense, Madrid, Spain.
Cascales
M
M
Boscá
L
L
D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis / Q000494:pharmacology; D005635:Fructosediphosphates / Q000378:metabolism*; D005934:Glucagon / Q000494:pharmacology; D005994:Glycerophosphates / Q000494:pharmacology; D006598:Hexosediphosphates / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008099:Liver / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D005635:Fructosediphosphates; D005994:Glycerophosphates; D006598:Hexosediphosphates; D007773:Lactates; D019344:Lactic Acid; C027652:fructose 2,6-diphosphate; D005934:Glucagon; C029620:alpha-glycerophosphoric acid; D000242:Cyclic AMP; D011494:Protein Kinases; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase