title: Three amino acids of a heptapeptide-induced antibody are responsible for recognition of SV40 structural proteins.
abstract: Antibodies were elicited against the heptapeptide Asp-His-Lys-Val-Ser-Thr-Val. The sequence of the heptapeptide corresponds to amino acid residues 105-111 of Simian Virus 40 (SV40) structural protein VP2. The antipeptide antibodies recognize VP2 and amino acid sequence Asp-His-Lys (193-195) in VP1 at different degrees of denaturation. Inhibition experiments were performed with the heptapeptide as well as with the tripeptide Asp-His-Lys. Using this model system, the specificity and selectivity of peptide-induced antibodies can be demonstrated with a resolution of a single amino acid.
authors.affiliation: Department of Medical Microbiology and Virology, Ruhr-University, Bochum, Federal Republic of Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Streckert
authors.forename: H
authors.initials: H
authors.lastname: Werchau
mesh_terms: D000596:Amino Acids / Q000276:immunology; D000818:Animals; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000276:immunology; D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits; D013539:Simian virus 40 / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*; D015678:Viral Structural Proteins
chemical_list: D000596:Amino Acids; D000956:Antigens, Viral; D009842:Oligopeptides; D014764:Viral Proteins; D015678:Viral Structural Proteins