[Inhibition of the transport of citrate during ADP-ribosylation of inner membrane proteins of the mitochondria].
The ability of rat liver submitochondrial particles to catalyze NAD+ hydrolysis with a transfer of ADP-ribose residues to protein membranes has been demonstrated ADP-ribosylation is directly dependent on NAD+ concentration upon saturation with 1 mM NAD+ and is inhibited by physiological compounds (e.g., ATP, 10 mM; nicotinamide, 10 mM); besides, it is an artificial acceptor of ADP-ribose, arginine methyl ester. It was found that ADP-ribose is accepted by inner mitochondrial membrane protein, whose molecular masses amount to 25-30 kDa. The fact that 5'-AMP is a product of ADP-ribose degradation by snake venom phosphodiesterase suggests that the inner membrane vesiculate proteins are modified by mono(ADP-ribose). Covalent modification of membrane proteins by ADP-ribose leads to citrate transport inhibition in inner membrane vesicles the [14C]citrate uptake is significantly decreased thereby. The ability of ADP-ribosylation inhibitors to restore the citrate transport rate is suggestive of a direct regulatory effect of NAD+-dependent ADP-ribosylation on the activity of citrate-translocating system of inner mitochondrial membranes.
Velikiĭ
N N
NN
Sen'ko
L N
LN
Boĭko
L M
LM
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002951:Citrates / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D013367:Submitochondrial Particles / Q000378:metabolism*
D002951:Citrates; D008565:Membrane Proteins; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate
Ingibirovanie transporta tsitrata pri ADP-ribozilirovanii belkov vnutrennikh membran mitokhondriĭ.