title: Molecular cloning and sequence analysis of the human parainfluenza 3 virus genes encoding the surface glycoproteins, F and HN.
abstract: The sequence of the genes encoding the fusion (F) and hemagglutinin-neuraminidase (HN) glycoproteins of the human parainfluenza 3 virus was determined by molecular cloning. The genes were cloned by primer extension using genomic 50 S RNA as the template. A series of four overlapping clones was generated from the 3' end of the fusion gene which extended across the gene end and intergenic boundaries of the F-HN and HN-L genes. The F gene extends 1851 nucleotides (inclusive of the putative transcription initiation and polyadenylation signals) and encodes a protein consisting of 539 amino acids (mol wt 60,067). This protein contains four potential sites for N-linked glycosylation in the F1 subunit polypeptide and none in the F2 subunit polypeptide. The lack of a potential site of glycosylation in F2 makes this protein unique compared to other reported paramyxoviral F proteins. The HN gene extends 1888 nucleotides and encodes a protein consisting of 572 amino acids (mol wt 64,255). This protein contains four potential sites for N-linked glycosylation.
authors.affiliation: Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, OH 45219.
authors.forename: M S
authors.initials: MS
authors.lastname: Galinski
authors.forename: M A
authors.initials: MA
authors.lastname: Mink
authors.forename: M W
authors.initials: MW
authors.lastname: Pons
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D015707:HN Protein; D006389:Hemagglutinins, Viral / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010224:Parainfluenza Virus 3, Human / Q000235:genetics*; D021382:Protein Sorting Signals; D018108:Respirovirus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D015707:HN Protein; D006389:Hemagglutinins, Viral; D021382:Protein Sorting Signals; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins