Bovine interleukin 2: production and characterization.
The production of bovine IL 2 was studied and IL 2 was partially characterized. PMA at 5 ng/ml + Concanavalin A at 5 micrograms/ml treatment of peripheral blood mononuclear cells gave a greater yield of IL 2 activity in the supernatants than Con A, PMA or sodium periodate treatments alone. Macrophage depletion increased yields as did the addition of indomethacin, a prostaglandin E2 inhibitor. Bovine IL 2 was sensitive to trypsin, relatively stable at pH 2-9, 2-ME resistant and sensitive to increasing molar concentrations of urea. The activity of bovine IL 2 was reduced by over 45% at 70 degrees C for 30 min and 95% at 90 degrees C for 30 min. Bovine IL 2 was more stable at 4 degrees C than at room temperature and the stability at room temperature could be improved by inclusion of 1% BSA. Bovine IL 2 eluted from DEAE-Sephadex as a broad peak with 0.1-0.2 M NaCl. Peak activity corresponded to a molecular weight of approximately 16,000 daltons on Sephadex G-100.
Sambhara
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Department of Molecular Biology, College of Agriculture, University of Wyoming, Laramie 82071.
Belden
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D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D003429:Cross Reactions; D004355:Drug Stability; D006168:Guinea Pigs; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000302:isolation & purification / Q000502:physiology; D010504:Periodic Acid / Q000494:pharmacology; D012756:Sheep; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D007376:Interleukin-2; D010504:Periodic Acid; D003208:Concanavalin A; C009288:metaperiodate; D014357:Trypsin; D013755:Tetradecanoylphorbol Acetate