title: Developmental activation of phosphoglycerate mutase-2 in the testis of the mouse.
abstract: The expression of the phosphoglycerate mutase locus Pgam-2 which synthesizes the muscle-specific PGAM-B subunit was analyzed in the testis of the mouse. No PGAM-B activity was detected in testes of newborn mice, in which only the PGAM-AA isozyme was observed. PGAM-B was first observed between Day 14 and Day 16 of postnatal development. In adult males approximately 50% of total PGAM activity is contributed by the PGAM-B subunit and 50% by the PGAM-A subunit. Immunohistochemical studies show that in the testis PGAM-B is localized exclusively in germ cells. PGAM-B is detected in pachytene spermatocytes and in spermatids, but not in earlier stages of spermatogenesis. The muscle-specific PGAM isozyme was also found in testes of bull, cat, and rat, as well as in human sperm. PGAM-B might thus be useful as a marker for germ cell differentiation, along with other germ cell-specific proteins.
authors.affiliation: Laboratory of Cell Differentiation, University of Geneva, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Fundele
authors.forename: H
authors.initials: H
authors.lastname: Winking
authors.forename: K
authors.initials: K
authors.lastname: Illmensee
authors.forename: E M
authors.initials: EM
authors.lastname: Jägerbauer
mesh_terms: D000367:Age Factors; D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D004789:Enzyme Activation; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D051379:Mice; D009132:Muscles / Q000201:enzymology; D010770:Phosphotransferases / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000201:enzymology / Q000254:growth & development*
chemical_list: D007527:Isoenzymes; D010770:Phosphotransferases; D001731:Bisphosphoglycerate Mutase