Indomethacin stimulation of macrophage cytostasis against MOPC-315 tumor cells is inhibited by both prostaglandin E2 and nordihydroguaiaretic acid, a lipoxygenase inhibitor.
Indomethacin enhanced macrophage cytostasis against MOPC-315 tumor cells in vitro. The effect of indomethacin was inhibited by prostaglandin E2 and by the lipoxygenase inhibitor nordihydroguaiaretic acid. Prostaglandin E2 and nordihydroguaiaretic acid also inhibited indomethacin stimulation of macrophage thymidine incorporation. Indomethacin inhibited macrophage prostaglandin E2 formation and stimulated leukotriene B4 synthesis. Nordihydroguaiaretic acid inhibited leukotriene B4 production. Our data indicate that eicosanoids play a role in regulating macrophage cytostasis.
Elliott
G R
GR
Department of Pharmacology, Medical Faculty, Erasmus University Rotterdam, The Netherlands.
Tak
C
C
Pellens
C
C
Ben-Efraim
S
S
Bonta
I L
IL
D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D015232:Dinoprostone; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D007975:Leukotriene B4 / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D009637:Masoprocol / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000473:pathology*; D011458:Prostaglandins E / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002396:Catechols; D011458:Prostaglandins E; D007975:Leukotriene B4; D013929:Thromboxane B2; D009637:Masoprocol; D015232:Dinoprostone; D013936:Thymidine; D007213:Indomethacin