Diagnosis of pseudorabies virus infection in pigs with specific DNA probes.
Two DNA probes directed against pseudorabies virus DNA were developed for use in a dot hybridisation to detect the presence of pseudorabies virus in infected tissues. The specificity of the probes and their sensitivity were analysed. There was a strong correlation between the results obtained by dot hybridisation and those obtained by conventional methods of virus isolation from the same tissue specimens.
Linné
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Department of Veterinary Microbiology, Biomedical Centre, Uppsala, Sweden.
D000818:Animals; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D011558:Herpesvirus 1, Suid / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D011557:Pseudorabies / Q000175:diagnosis*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*
D004279:DNA, Viral