The effect of calcium ionophore A23187 on the metabolism of arachidonic and eicosapentaenoic acids in neutrophils from a marine teleost fish rich in (n-3) polyunsaturated fatty acids.
1. [1-14C]AA and [1-14C]EPA were incorporated equally into plaice neutrophil phospholipids. 2. Incubation with A23187 resulted in the loss of label from total phospholipids and increased label released from the neutrophils. 3. Labelled LTB4 and LTB5 production was increased 2.5- and 3-fold, respectively, by A23187 treatment. 4. However, the incorporated AA was generally more metabolically active than the incorporated EPA with approx. 3-4 times as much LTB4 produced than LTB5 by cells in the presence or absence of A23187, respectively. 5. The results obtained in this (n-3) PUFA-rich species were discussed in comparison with current knowledge of AA and EPA metabolism in mammalian neutrophils.
Tocher
D R
DR
Natural Environment Research Council, Institute of Marine Biochemistry, Aberdeen, UK.
Sargent
J R
JR
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D000001:Calcimycin / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D015118:Eicosapentaenoic Acid / Q000097:blood*; D005412:Flatfishes / Q000097:blood*; D007700:Kinetics; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis / Q000097:blood*
D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D010743:Phospholipids; D016718:Arachidonic Acid; D000001:Calcimycin; D015118:Eicosapentaenoic Acid