Rat epiphyseal cells in culture: responsiveness to bone-seeking hormones.
Cell cultures derived from young rat epiphyseal cartilage were grown for approximately 2 wk in BGJb medium supplemented with 10% fetal bovine serum to reach confluence. These cells were identified as chondrocytes as checked by morphology, the presence of alkaline phosphatase, and a positive type II collagen antibody reaction. The cells also responded to different hormonal treatment. Parathyroid hormone (PTH) increased cyclic AMP production by 50% within 15 min of treatment, whereas prostaglandin E2 (PGE2) caused an increase of 160%. Calcitonin (CT) did not affect cAMP production in these cells. DNA synthesis 24 h after hormonal treatment was increased by PTH (2.5-fold) and PGE2 (2-fold), but not by CT. Among the vitamin D metabolites, 24,25(OH)2D3 increased significantly the [3H]thymidine incorporation into DNA, whereas 1,25(OH)2D3 effect was minimal. These results provide evidence for the use of these cell cultures as a model for cartilage in vitro when studying biological and hormonal responsiveness.
Levy
J
J
Department of Orthodontics, Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Israel.
Shimshoni
Z
Z
Somjen
D
D
Berger
E
E
Fine
N
N
Silbermann
M
M
Binderman
I
I
D015650:24,25-Dihydroxyvitamin D 3; D000818:Animals; D002116:Calcitonin / Q000494:pharmacology*; D002117:Calcitriol / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D004100:Dihydroxycholecalciferols / Q000494:pharmacology; D015232:Dinoprostone; D004838:Epiphyses / Q000187:drug effects / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D014807:Vitamin D / Q000494:pharmacology*
D004100:Dihydroxycholecalciferols; D010281:Parathyroid Hormone; D011458:Prostaglandins E; D014807:Vitamin D; D015650:24,25-Dihydroxyvitamin D 3; D002116:Calcitonin; D004247:DNA; D000242:Cyclic AMP; D002117:Calcitriol; D015232:Dinoprostone