Insertion of a retrotransposon within the 3' end of a mouse gene provides a new functional polyadenylation signal.
A site of genomic insertion of the mouse retrotransposon LTR-IS/MuRRS was analysed. The comparison of the genomic and the cDNA clones indicates the insertion of the LTR-IS element into the 3' untranslated region of a mouse gene. The fact that the isolated cDNA clone ends with a poly A tail 20 nucleotides downstream from the LTR-IS AATAAA box and the result of the S1-nuclease mapping provides evidence that the 3' end of the mouse gene transcript was generated under the control of the LTR-IS polyadenylation signal.
Baumruker
T
T
Institut für Virologie und Immunbiologie der Universität Würzburg, FRG.
Gehe
C
C
Horak
I
I
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA