title: The highly conserved amino-terminal region of the protein encoded by the v-myb oncogene functions as a DNA-binding domain.
abstract: The retroviral oncogene v-myb encodes a 45,000 Mr nuclear protein (p45v-myb) that is predominantly associated with the chromatin of transformed cells. It has previously been shown that p45v-myb, when released from chromatin by salt-treatment, binds to DNA. To analyse the biochemical properties of p45v-myb in more detail we have expressed the v-myb coding region in Escherichia coli. Our results demonstrate that bacterially expressed myb protein has an intrinsic DNA-binding activity. Using two alternative strategies, (i) inhibition of DNA-binding by monoclonal antibodies and (ii) analysis of DNA-binding activities of partially deleted forms of the bacterial myb protein, we show that the DNA-binding domain is located in the amino-terminal region of the v-myb protein. This region has been highly conserved between myb genes of different species. Our results are therefore consistent with the hypothesis that DNA-binding is an important aspect of myb protein function.
authors.affiliation: Zentrum für Molekulare Biologie, Universität Heidelberg, FRG.
authors.forename: K H
authors.initials: KH
authors.lastname: Klempnauer
authors.forename: A E
authors.initials: AE
authors.lastname: Sippel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D009857:Oncogenes; D010957:Plasmids
chemical_list: D004268:DNA-Binding Proteins; D004262:DNA Restriction Enzymes