title: Isolation and characterization of alphoid DNA sequences specific for the pericentric regions of chromosomes 4, 5, 9, and 19.
abstract: We have cloned and characterized two distinct types of alphoid DNA elements. Probe pG-Xba 11/340 was obtained by random cloning of human satellite DNA and contains two basic units with overall 88% homology to the 171-bp consensus alphoid sequence. pG-Xba 11/340-like elements are represented about 2,000-4,000 times in the haploid genome and, by in situ hybridization, are found exclusively at the primary constrictions of chromosomes 4 and 9. Probe pG-A16 was cloned from a chromosome 19-specific cosmid library and represents a 2.25-kb higher-order DNA element which is present at roughly 75-150 copies per haploid genome and which hybridizes to the centromeres of chromosomes 5 and 19. Using the pG-A16 probe, further genetic and physical dissection of the central area of chromosome 19 can be envisaged.
authors.affiliation: Department of Human Genetics, University of Nijmegen, The Netherlands.
authors.forename: T
authors.initials: T
authors.lastname: Hulsebos
authors.forename: D
authors.initials: D
authors.lastname: Schonk
authors.forename: I
authors.initials: I
authors.lastname: van Dalen
authors.forename: M
authors.initials: M
authors.lastname: Coerwinkel-Driessen
authors.forename: J
authors.initials: J
authors.lastname: Schepens
authors.forename: H H
authors.initials: HH
authors.lastname: Ropers
authors.forename: B
authors.initials: B
authors.lastname: Wieringa
mesh_terms: D001483:Base Sequence; D002503:Centromere; D002905:Chromosomes, Human, 4-5; D002888:Chromosomes, Human, Pair 19; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004276:DNA, Satellite; D004262:DNA Restriction Enzymes