Inhibition of protein synthesis does not antagonize induction of UV-induced sister-chromatid exchange in xeroderma pigmentosum cells.
Cycloheximide strongly antagonizes the induction of sister-chromatid exchanges by ethyl methanesulfonate or mitomycin C in human skin fibroblast and xeroderma pigmentosum cells (group A). Analogous behavior has been observed in several other species including Chinese hamster and plant cells. This report documents an exception to that pattern: cycloheximide fails to antagonize UV-induced sister chromatid exchange in xeroderma pigmentosum cells, whereas it does in normal human skin fibroblast cells. A genetic defect in these cells is postulated to alter the UV-mediated DNA recombination process.
Sono
A
A
Division of Toxicology, Toyo Jozo Co. Ltd., Shizuoka-ken, Japan.
Sakaguchi
K
K
D002478:Cells, Cultured; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D008698:Mesylates / Q000494:pharmacology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics* / Q000473:pathology
D008698:Mesylates; D008937:Mitomycins; D009153:Mutagens; C045880:methanesulfonic acid; D016685:Mitomycin