title: A herpes simplex virus type 1 mutant resistant to benzhydrazone, an inhibitor of glycoprotein synthesis in herpesvirus-infected cells. Preliminary mapping of benzhydrazone-resistance and of a novel syncytial mutation.
abstract: Benzhydrazone (BH) is an inhibitor of glycoprotein biosynthesis. It acts selectively in Herpes simplex virus (HSV)-infected cells and does not significantly affect glycoprotein synthesis in uninfected cells and in cells infected with other viruses. Previously, we reported on a syncytial (syn) mutant, designated HSV-1(13)S11, resistant to BH, and showed that BH-resistance is encoded in the mutant virus DNA and therefore can be transferred into the genome of wild type HSVs. The present paper reports on a preliminary mapping in HSV-1(13)S11 genome of the loci which confer resistance to BH and of three distinct syn mutations present simultaneously in this mutant. Two of them were mapped in previously described syn loci localized in BamHI fragment L (map units 0.707-0.745) (locus syn 1) and BamHI fragment Q (map units 0.296-0.317) (locus syn 5). A third mutation not described before and mapping in BamHI fragment SP (c.a. map units 0.81-0.85) conferred the syn phenotype to both HEp-2 and Vero cells. This novel mutation has been designated herein locus syn 6. Transfer of BH-resistance could be achieved in cotransfection experiments involving two HSV-1(13)S11 fragments, BamHIL and BamHISP.
authors.affiliation: Institute of Microbiology, University of Ferrara, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Tognon
authors.forename: R
authors.initials: R
authors.lastname: Manservigi
authors.forename: M G
authors.initials: MG
authors.lastname: Romanelli
authors.forename: A
authors.initials: A
authors.lastname: Rotola
authors.forename: R
authors.initials: R
authors.lastname: Gatti
authors.forename: L
authors.initials: L
authors.lastname: Foà-Tomasi
authors.forename: G
authors.initials: G
authors.lastname: Campadelli-Fiume
mesh_terms: D000818:Animals; D002459:Cell Fusion; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics; D004352:Drug Resistance, Microbial; D005814:Genes, Viral; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D006835:Hydrazones / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D004279:DNA, Viral; D006023:Glycoproteins; D006835:Hydrazones; D014764:Viral Proteins; C027566:benzhydrazone