Duck hepatitis B virus (DHBV) particles produced by transient expression of DHBV DNA in a human hepatoma cell line are infectious in vitro.
Transfection of the human hepatocellular carcinoma cell line HuH7 with a plasmid containing a tandem copy of the duck hepatitis B virus DNA sequence resulted in transient replication of the virus. Viral particles secreted by transfected HuH7 cells exhibited physical properties similar to those of serum-derived duck hepatitis B virus and were infectious in primary duck hepatocyte cultures.
Pugh
J C
JC
Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111.
Yaginuma
K
K
Koike
K
K
Summers
J
J
D000818:Animals; D006528:Carcinoma, Hepatocellular; D002478:Cells, Cultured; D004279:DNA, Viral / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008099:Liver / Q000382:microbiology; D008113:Liver Neoplasms; D009693:Nucleic Acid Hybridization; D014162:Transfection; D014407:Tumor Cells, Cultured; D014771:Virion / Q000502:physiology*; D014779:Virus Replication
D004279:DNA, Viral