Molecular cloning and nucleotide sequence of the pectin methyl esterase gene of Erwinia chrysanthemi B374.
The gene encoding pectin methyl esterase (pme) has been cloned from Erwinia chrysanthemi B374. Expression of pme in Escherichia coli allowed the enzyme to be characterized. Pectin methyl esterase (PME) was found to have an apparent molecular weight of 36,000 Daltons and an isoelectric point of approximately 9.9. The structural gene was sequenced and consists of a 1098-bp open reading frame encoding a polypeptide of 39,318 Daltons, which includes an amino-terminal signal peptide. The isolation of the Erwinia gene provides a simple method for the production of PME free from depolymerizing pectinases thereby extending its potential uses.
Plastow
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Dalgety UK Ltd, Group Research Laboratory, Cambridge, UK.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004885:Erwinia / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping
D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D004262:DNA Restriction Enzymes