[Comparison of 2 immunoenzyme methods for the determination of anti-cytomegalovirus IgM].
An antibody capture assay technique using a labeled antigen (Wellcome Laboratories) and an ELISA technique performed after elimination of serum IgG (Behring Laboratories) were assessed for determination of anti-cytomegalovirus IgM. Their specificity and sensitivity were compared in tests on 208 sera, some (60) of which had IgM rheumatoid factor, antinuclear antibodies or anti-Epstein-Barr virus antibodies. The other sera were analyzed relative to diagnosis of a cytomegalovirus infection. The antibody capture assay technique was slightly more sensitive than the ELISA technique but did not resolve all the specificity problems.
Billaudel
S
S
Laboratoire de Virologie, UFR de Médecine, Nantes.
Besse
B
B
Mézière
A
A
Courtieu
A L
AL
D000974:Antibodies, Antinuclear / Q000032:analysis; D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*; D012217:Rheumatoid Factor / Q000032:analysis
D000974:Antibodies, Antinuclear; D000914:Antibodies, Viral; D007075:Immunoglobulin M; D012217:Rheumatoid Factor
Comparaison de deux méthodes immunoenzymatiques pour la détermination des IgM anti-cytomegalovirus.