An enzyme-linked immunosorbent assay for the detection of antibodies to avian reticuloendotheliosis virus using whole cell antigen.
An enzyme-linked immunosorbent assay (ELISA) using reticuloendotheliosis virus-infected chick embryo fibroblasts as coating antigen is described for the detection of antibodies to reticuloendotheliosis virus in chicken sera. The ELISA was specific and during the early stages of infection more sensitive than an indirect fluorescent antibody test.
Nicholas
R A
RA
Central Veterinary Laboratory, Weybridge, Surrey.
Thornton
D H
DH
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D001356:Reticuloendotheliosis virus / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*
D000914:Antibodies, Viral