[Isolation and characteristics of clones complementary to mRNA of the murine cellular tumor antigen p53].
43 cDNA clones specific for murine cellular tumor antigen p53 were isolated from a library constructed using 17S fraction of mRNA from the SV40 transformed murine fibroblasts (SVT2). These clones contain the whole coding region of p53 mRNA and the most of non-translated sequences. A plasmid containing 1.8 kb insert of p53 cDNA was constructed. The p53 specific insert in this plasmid was colinear with p53 mRNA, as revealed by S1 nuclease analysis. 5'-region of the p53 gene comprising non-translated and promoter areas was cloned from the mouse genomic library. A combined clone containing promoter and the whole region, corresponding to p53 mRNA has been constructed.
Chumakov
P M
PM
Iotsova
V S
VS
Ninkina
N N
NN
Bukhman
V L
VL
Georgiev
G P
GP
D000818:Animals; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005347:Fibroblasts; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010750:Phosphoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D013539:Simian virus 40; D016159:Tumor Suppressor Protein p53
D009363:Neoplasm Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D004247:DNA; D004262:DNA Restriction Enzymes
Vydelenie i kharakteristika klonov, komplementarnykh mRNK myshinogo kletochnogo opukholevogo antigena p53.