title: The synthesis, physical characterization and receptor binding affinity of neuropeptide Y (NPY).
abstract: Porcine neuropeptide Y (pNPY) was synthesized by solid-phase techniques and purified by gel filtration and reverse-phase high performance liquid chromatography. It was found to be equipotent to commercial pNPY (Peninsula) in a receptor binding assay (IC50 = 5 nM). Circular dichroic (CD) spectra of the peptide indicates a concentration-independent alpha-helical conformation in aqueous solution ([theta]222 = -13,998, 2.9 X 10(-5) M in 0.1 M sodium phosphate, pH = 8.0). Lowering the pH of the solution to 6.0 produced little change in the CD spectrum, but CD spectra at pH = 4.2 and 1.8 indicated a loss of alpha-helical content of the peptide with decreasing pH. Sedimentation equilibria of synthetic pNPY showed that it is neither wholly monomeric nor dimeric at pH = 4.2 and 8.0. These data suggest an intramolecularly stabilized helical structure similar to the crystal structure of the homologous avian pancreatic polypeptide (APP).
authors.affiliation: Merrell Dow Research Institute, Cincinnati, Ohio 45215.
authors.forename: J L
authors.initials: JL
authors.lastname: Krstenansky
authors.forename: S H
authors.initials: SH
authors.lastname: Buck
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D005399:Fishes; D006801:Humans; D008433:Mathematics; D009478:Neuropeptide Y / Q000138:chemical synthesis* / Q000378:metabolism; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D013552:Swine
chemical_list: D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter