[Site-specific endonuclease CauB31 from Chloroflexus aurantiacus B3].
A sequence-specific endonuclease CauB3I has been isolated from cell extracts of Chloroflexus aurantiacus and partially purified by chromatography on heparin-sepharose; the yield was 3000 units per 1 g of cells. The final preparation is free of non-specific nucleases. It is shown that endonuclease CauB3I recognizes 5' T decreases CCGGA 3' sequence in double-stranded DNA and cleaves it as shown by an arrow. Methylation of adenine in the recognition sequence makes it resistant to CauB3I.
Kramarov
V M
VM
Fomenkov
A I
AI
Matvienko
N I
NI
Ubieta
R Kh
RKh
Smolianinov
V V
VV
D001483:Base Sequence; D000460:Chlorophyta / Q000201:enzymology*; D002847:Chromatography, Agarose; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D010957:Plasmids; D012685:Sepharose / Q000031:analogs & derivatives
D004269:DNA, Bacterial; C037405:heparin-sepharose; D012685:Sepharose; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C053919:TCCGGA-specific type II deoxyribonucleases
Saĭt-spetsificheskaia éndonukleaza CauB31 iz Chloroflexus aurantiacus B3.