title: Isolation and restriction endonuclease analysis of a mycoplasma plasmid.
abstract: A 1.7-kb plasmid was isolated from a large colony type strain of Mycoplasma mycoides subsp. mycoides. It was cloned into the Escherichia coli vector M13mp19, and DNA from the resultant recombinant was used to construct a restriction map of the plasmid. Because mycoplasmas show deviation from normal coding patterns, the availability of a mycoplasma plasmid could be useful in the development of a cloning vector for them.
authors.affiliation: Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: A D
authors.initials: AD
authors.lastname: Bergemann
authors.forename: L R
authors.initials: LR
authors.lastname: Finch
mesh_terms: D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D009174:Mycoplasma / Q000235:genetics*; D009710:Nucleotide Mapping; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes