title: Human-milk intake measured by administration of deuterium oxide to the mother: a comparison with the test-weighing technique.
abstract: A comparison was made between the dose-to-the-mother deuterium-dilution method and the conventional test-weighing technique for determining human-milk intake in five exclusively breast-fed infants and in four breast-fed infants who received supplemental foods. After administration of 2H to the mothers human milk and infant urine were sampled over 14 d and analyzed for 2H:1H ratios by gas-isotope-ratio mass spectrometry. Infant total body water was determined by 18O dilution. The test-weighing procedure was conducted for 5 d consecutively. The intake of human milk (mean +/- SD) estimated by 2H dilution was 648 +/- 63 g/d and estimated by test-weighing was 636 +/- 84 g/d. The mean difference between the two methods was not significantly different from 0. The 2H-dilution and test-weighing techniques provide similar estimates of human-milk intake.
authors.affiliation: US Department of Agriculture, Children's Nutrition Research Center, Houston, TX 77030.
authors.forename: N F
authors.initials: NF
authors.lastname: Butte
authors.forename: W W
authors.initials: WW
authors.lastname: Wong
authors.forename: B W
authors.initials: BW
authors.lastname: Patterson
authors.forename: C
authors.initials: C
authors.lastname: Garza
authors.forename: P D
authors.initials: PD
authors.lastname: Klein
mesh_terms: D000328:Adult; D001834:Body Water / Q000378:metabolism; D001835:Body Weight; D001942:Breast Feeding; D003903:Deuterium; D017666:Deuterium Oxide; D005260:Female; D006801:Humans; D007225:Infant Food; D007774:Lactation; D008895:Milk, Human; D011247:Pregnancy; D011865:Radioisotope Dilution Technique; D014867:Water
chemical_list: D014867:Water; D003903:Deuterium; D017666:Deuterium Oxide