title: Preliminary X-ray diffraction analysis of HhaII endonuclease-DNA cocrystals.
abstract: HhaII restriction endonuclease purified from an overproducing recombinant E. coli clone has been cocrystallized with a heptanucleotide duplex, d-GGAGTCC:GGACTCC. The cocrystals are monoclonic and belong to the space group C2. The unit cell dimensions are a = 199.0 +/- 1.0 A, b = 100.0 +/- 0.5 A, c = 80.3 +/- 0.4 A, and beta = 101.0 +/- 1.0 degrees. There appear to be two dimers per asymmetric unit and the crystals diffract to 4-A resolution.
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: S
authors.initials: S
authors.lastname: Chandrasegaran
authors.forename: H O
authors.initials: HO
authors.lastname: Smith
authors.forename: M L
authors.initials: ML
authors.lastname: Amzel
authors.forename: X
authors.initials: X
authors.lastname: Ysern
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D004247:DNA; D004262:DNA Restriction Enzymes / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics; D009838:Oligodeoxyribonucleotides; D014961:X-Ray Diffraction
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases