title: HIV infection is blocked in vitro by recombinant soluble CD4.
abstract: The T-cell surface glycoprotein, CD4 (T4), acts as the cellular receptor for human immunodeficiency virus, type 1 (HIV-1), the first member of the family of viruses that cause acquired immunodeficiency syndrome. HIV recognition of CD4 is probably mediated through the virus envelope glycoprotein (gp120) as shown by co-immunoprecipitation of CD4 and gp120 (ref.5) and by experiments using recombinant gp120 as a binding probe. Here we demonstrate that recombinant soluble CD4(rsT4) purified from the conditioned medium of a stably transfected Chinese hamster ovary cell line is a potent inhibitor of both virus replication and virus-induced cell fusion (syncytium formation). These results suggest that rsT4 is sufficient to bind HIV, and that it represents a potential anti-viral therapy for HIV infection.
authors.affiliation: Biogen Research Corporation, Cambridge, Massachusetts 02142.
authors.forename: R A
authors.initials: RA
authors.lastname: Fisher
authors.forename: J M
authors.initials: JM
authors.lastname: Bertonis
authors.forename: W
authors.initials: W
authors.lastname: Meier
authors.forename: V A
authors.initials: VA
authors.lastname: Johnson
authors.forename: D S
authors.initials: DS
authors.lastname: Costopoulos
authors.forename: T
authors.initials: T
authors.lastname: Liu
authors.forename: R
authors.initials: R
authors.lastname: Tizard
authors.forename: B D
authors.initials: BD
authors.lastname: Walker
authors.forename: M S
authors.initials: MS
authors.lastname: Hirsch
authors.forename: R T
authors.initials: RT
authors.lastname: Schooley
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D001667:Binding, Competitive; D002459:Cell Fusion; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006678:HIV / Q000502:physiology*; D015699:HIV Envelope Protein gp120; D010053:Ovary; D010446:Peptide Fragments / Q000378:metabolism; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D012191:Retroviridae Proteins / Q000378:metabolism; D012995:Solubility; D014779:Virus Replication
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D015699:HIV Envelope Protein gp120; D010446:Peptide Fragments; D015728:Receptors, HIV; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D012191:Retroviridae Proteins