A comparative study of virulent and avirulent strains of Chromobacterium violaceum.
A clinical isolate and a soil isolate of Chromobacterium violaceum were compared to determine differences in virulence-related characteristics. Purified lipopolysaccharide (endotoxin) from the virulent, clinical strain was more reactive than that from the avirulent soil strain as determined by the Limulus amebocyte lysate assay. There were no differences in hemolysin or cyanide production between the two strains. The virulent strain was more resistant to phagocytosis and intracellular killing by human polymorphonucleocytes. The clinical strain showed a superoxide dismutase activity 30% higher and a catalase activity fivefold higher than the activities of the soil-isolated strain. The clinical strain also was capable of producing approximately twice as much hydrogen peroxide during growth as compared with the soil isolate. This study suggests that virulence of C. violaceum may be, at least in part, associated with endotoxin, and some protection of the virulent, clinical strain from phagocytic attack is afforded by elevated levels of superoxide dismutase and catalase.
Miller
D P
DP
Department of Botany and Microbiology, College of Sciences and Mathematics, Auburn University, AL 36849.
Blevins
W T
WT
Steele
D B
DB
Stowers
M D
MD
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002861:Chromobacterium / Q000201:enzymology / Q000276:immunology / Q000472:pathogenicity*; D003486:Cyanides / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000378:metabolism; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis; D012988:Soil Microbiology; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014774:Virulence
D003486:Cyanides; D008070:Lipopolysaccharides; D013481:Superoxides; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase