title: Cloned extrachromosomal circular DNA copies of the human transposable element THE-1 are related predominantly to a single type of family member.
abstract: The 2300 base-pair transposon-like human element, THE-1, has been identified in the extrachromosomal circular DNA of the established human cell line HeLa as a relatively homogeneous population of covalently closed 1900 base-pair molecules. THE-1, which has been classified tentatively as a retroviral-like transposable element (a retrotransposon), is present in the extrachromosomal circular DNA of African green monkey (BSC-1) and human lymphoblastoid (Jurkat) cell lines. The 1900 base-pair extrachromosomal elements isolated and cloned from HeLa cells (1) appear to contain only THE-1-specific nucleotide sequences, (2) are circularized versions of the linear chromosomal sequence, and (3) are related predominantly to a single, or single type of, family member.
authors.affiliation: Department of Biochemistry, New York University School of Medicine, NY 10016.
authors.forename: R
authors.initials: R
authors.lastname: Misra
authors.forename: A
authors.initials: A
authors.lastname: Shih
authors.forename: M
authors.initials: M
authors.lastname: Rush
authors.forename: E
authors.initials: E
authors.lastname: Wong
authors.forename: C W
authors.initials: CW
authors.lastname: Schmid
mesh_terms: D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004270:DNA, Circular; D005111:Extrachromosomal Inheritance; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D004251:DNA Transposable Elements; D004270:DNA, Circular