Identification of Marek's disease virus nuclear antigen in latently infected lymphoblastoid cells.
A new Marek's disease virus (MDV) nuclear antigen (MDNA) was identified in two MDV-transformed T-lymphoblastoid cell lines, MKT-1 and MSB-1, derived from chickens bearing tumors induced by MDV. This MDNA was not detected in MSB-1 cells maintained in iododeoxyuridine, which activates the latent MDV genome. Moreover, it was not found in chicken embryo fibroblasts undergoing productive and cytolytic infection with MDV. Expression of MDNA is not related to strain pathogenicity in chickens, because chicken embryo fibroblasts productively infected with the pathogenic RBIB strain or the nonpathogenic CV-1 strain of MDV did not express this antigen. DNA-protein immunoprecipitation studies revealed that MDNA bound to two sites in the 190,00-base-pair (bp) MDV genome. One of these loci identified by MDNA obtained from MKT-1 and MSB-1 cells corresponded to a 476-bp segment within the short unique region of BamHI-A MDV DNA. A second locus located in a 280-bp segment within the short inverted repeat region of BamHI-A was also identified by MDNA from MSB-1 cells but not by MDNA obtained from MKT-1 cells. Analyses of the nucleotide sequence by DNase digestion showed that MDNA protected a 60-bp segment spanning a 22-bp palindromic sequence of the short unique region and a 103-bp sequence encompassing a 32-bp palindrome in the short inverted repeat region of BamHI-A MDV DNA.
Wen
L T
LT
Department of Virology, Showa University Research Institute for Biomedicine, St. Petersburg, Florida 33716.
Tanaka
A
A
Nonoyama
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M
D000818:Animals; D034961:Antigens, Nuclear; D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D002461:Cell Line, Transformed; D002845:Chromatography; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000276:immunology*; D007118:Immunoassay; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000276:immunology*
D034961:Antigens, Nuclear; D000956:Antigens, Viral; D004279:DNA, Viral; D009687:Nuclear Proteins