Abnormal rabbit heterophil chemotaxis following thermal injury. An in vivo model of an abnormality of the chemoattractant receptor for f-met-leu-phe.
Previous studies have shown that the decreased neutrophil migratory responsiveness seen in burned patients correlates with the extent of thermal injury and the extent of the neutrophil-specific granule deficiency. To understand better the relationship between the neutrophil dysfunction, degranulation, and thermal injury, a rabbit model was studied. Eighteen rabbits were burned over 20% of their surface area. Assay of peripheral blood heterophils disclosed decreased migratory activity compared with preburn levels and decreased lysozyme content vs preburn levels, but no change in the beta-glucuronidase content. The specific binding of tritiated formyl-methionyl-leucyl-phenylalanine to peripheral blood heterophils was increased fivefold over that of control cells. These studies indicate that, following thermal injury, there is a selective decrease of specific granule contents and an increase in chemoattractant binding to the cell and also suggest an abnormality in chemoattractant receptor processing. The rabbit provides a convenient model for the study of compromised host defenses following thermal injury.
Davis
J M
JM
Department of Surgery, Cornell University Medical College, New York.
Gallin
J I
JI
D000818:Animals; D001835:Body Weight; D002056:Burns / Q000097:blood* / Q000378:metabolism / Q000382:microbiology; D002464:Cell Migration Inhibition; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004195:Disease Models, Animal; D004731:Endotoxins / Q000493:pharmacokinetics; D004927:Escherichia coli Infections / Q000097:blood; D009113:Muramidase / Q000032:analysis; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology; D009842:Oligopeptides / Q000378:metabolism / Q000502:physiology*; D011817:Rabbits; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000378:metabolism / Q000502:physiology*; D013203:Staphylococcal Infections / Q000097:blood; D013997:Time Factors
D004731:Endotoxins; D009842:Oligopeptides; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; C044101:endotoxin, Escherichia coli; D009113:Muramidase