Identification of healed terminal DNA fragments in linear minichromosomes of Schizosaccharomyces pombe.
The minichromosome Ch16 of the fission yeast Schizosaccharomyces pombe is derived from the centromeric region of chromosome III. We show that Ch16 and a shorter derivative, Ch12, made by gamma-ray cleavage, are linear molecules of 530 and 280 kilobases, respectively. Each minichromosome has two novel telomeres, as shown by genomic Southern hybridization with an S. pombe telomere probe. Comparison by hybridization of the minichromosomes and their chromosomal counterparts showed no signs of gross rearrangement. Cosmid clones covering the ends of the long arms of Ch16 and Ch12 were isolated, and subcloned fragments that contained the breakage sites were identified. They are apparently unique in the genome. By hybridization and Bal 31 digestion, the ends appear to consist of the broken-end sequences directly associated with short stretches (about 300 base pairs) of new DNA that hybridizes to a cloned S. pombe telomere. They do not contain the telomere-adjacent repeated sequences that are present in the normal chromosomes. The sizes of the short telomeric stretches are roughly the same as those of the normal chromosomes. Our results show that broken chromosomal ends in S. pombe can be healed by the de novo addition of the short telomeric repeats. The formation of Ch16 must have required two breakage-healing events, whereas a single cleavage-healing event in the long arm of Ch16 yielded Ch12.
Matsumoto
T
T
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Fukui
K
K
Niwa
O
O
Sugawara
N
N
Szostak
J W
JW
Yanagida
M
M
D002875:Chromosomes / Q000032:analysis*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000032:analysis*; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D009693:Nucleic Acid Hybridization; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*
D004271:DNA, Fungal; D004274:DNA, Recombinant; D005819:Genetic Markers; D004262:DNA Restriction Enzymes