title: Complete and partial ureteral obstruction: evaluation of renal effects with P-31 MR spectroscopy and Tc-DMSA scintigraphy.
abstract: Phosphorus-31 magnetic resonance (MR) spectroscopy was used to study the effects of partial and complete ureteral obstruction on the porcine kidney; results were compared with renal tubular function as determined with technetium-99m dimercaptosuccinic acid (DMSA) scintigraphy. Twenty-seven pigs were used: nine as sham-operated controls, six with partial ureteral obstruction, and 12 with complete ureteral obstruction. P-31 MR spectra and Tc-DMSA scintiscans were obtained weekly over 3 weeks. Partial obstruction caused no significant change in P-31 MR spectra, whereas Tc-DMSA scintiscans showed a 74% decrease in tubular function by the end of 3 weeks. Complete obstruction caused a 43% reduction in the mean adenosine triphosphate (ATP) to inorganic phosphate (Pi) ratio, which paralleled the 96% decrease in Tc-DMSA uptake over 3 weeks. The difference in ATP/Pi ratios between control and completely obstructed kidneys was significant (P less than .01) at 2 and 3 weeks after ligation. These results indicate that radionuclide Tc-99m DMSA uptake is very sensitive to the pathophysiologic changes in renal tubular function, while the organ average cellular bioenergetic state (ATP/Pi) is not as strongly affected.
authors.affiliation: Department of Pharmaceutical Chemistry, University of California, San Francisco 94143.
authors.forename: D B
authors.initials: DB
authors.lastname: Vigneron
authors.forename: A A
authors.initials: AA
authors.lastname: Tzika
authors.forename: H
authors.initials: H
authors.lastname: Hricak
authors.forename: D C
authors.initials: DC
authors.lastname: Price
authors.forename: P
authors.initials: P
authors.lastname: Bretan
authors.forename: S
authors.initials: S
authors.lastname: Aboseif
authors.forename: S
authors.initials: S
authors.lastname: Müller
authors.forename: T L
authors.initials: TL
authors.lastname: James
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D007668:Kidney / Q000378:metabolism*; D007674:Kidney Diseases / Q000000981:diagnostic imaging / Q000209:etiology*; D007684:Kidney Tubules / Q000000981:diagnostic imaging*; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds; D010710:Phosphates / Q000378:metabolism; D011877:Radionuclide Imaging; D004113:Succimer; D013552:Swine; D013556:Swine, Miniature; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D014517:Ureteral Obstruction / Q000150:complications*
chemical_list: D009942:Organometallic Compounds; D010710:Phosphates; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D000255:Adenosine Triphosphate; D004113:Succimer