title: Physical mapping of the Mycoplasma pneumoniae genome.
abstract: In order to study the genome organization of Mycoplasma pneumoniae a cosmid library of M. pneumoniae DNA was established using a newly designed cosmid vector (pcosRW2). From this library 32 overlapping clones were isolated covering a contiguous 720 kbp DNA segment representing about 90% of the genome assuming a genome size of about 800 kbp.
authors.affiliation: University of Heidelberg, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Wenzel
authors.forename: R
authors.initials: R
authors.lastname: Herrmann
mesh_terms: D001483:Base Sequence; D003360:Cosmids; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis
chemical_list: D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes