Localization of the active gene of aldolase on chromosome 16, and two aldolase A pseudogenes on chromosomes 3 and 10.
Southern blot analysis of human genomic DNA hybridized with a coding region aldolase A cDNA probe (600 bases) revealed four restriction fragments with EcoRI restriction enzyme: 7.8 kb, 13 kb, 17 kb and greater than 30 kb. By human-hamster hybrid analysis (Southern technique) the principal fragments, 7.8 kb, 13 kb, greater than 30 kb, were localized to chromosomes 10, 16 and 3 respectively. The 17-kb fragment was very weak in intensity; it co-segregated with the greater than 30-kb fragment and is probably localized on chromosome 3 with the greater than 30-kb fragment. Analysis of a second aldolase A labelled probe protected against S1 nuclease digestion by RNAs from different hybrid cells, indicated the presence of aldolase A mRNAs in hybrid cells containing only chromosome 16. Under the stringency conditions used, the EcoRI sequences detected by the coding region aldolase A cDNA probe did not correspond to aldolase B or C. The 7.8-kb and greater than 30-kb EcoRI sequences, localized respectively on chromosomes 10 and 3, correspond to aldolase A pseudogenes; the 13-kb EcoRI sequence localized on chromosome 16 corresponds to the aldolase active gene. The fact that the aldolase A gene and pseudogenes are located on three different chromosomes supports the hypothesis that the pseudogenes originated from aldolase A mRNAs, copied into DNA and integrated in unrelated chromosomal loci.
Serero
S
S
Clinique et Unité de Recherches de Génétique Médicale (INSERM U12), Hôpital des Enfants-Malades, Paris, France.
Maire
P
P
Nguyen
V C
VC
Cohen-Haguenauer
O
O
Gross
M S
MS
Jégou-Foubert
C
C
de Tand
M F
MF
Kahn
A
A
Frézal
J
J
D000818:Animals; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D011544:Pseudogenes
D005819:Genetic Markers; D004247:DNA; D004262:DNA Restriction Enzymes; D005634:Fructose-Bisphosphate Aldolase