title: Mouse cell lines that use heat shock promoters to regulate the expression of tissue plasminogen activator.
abstract: The promoters from Drosophila and human 70,000-dalton heat shock protein (hsp70) genes were linked to human tissue plasminogen activator (tPA) cDNA. Mouse C127 cells were transformed with bovine papilloma virus (BPV) vectors carrying the hybrid hsp70/tPA genes. Stable BPV-transformed cell lines were selected and analyzed for tPA expression before and after heat shock. In most cell lines, there was a low level of tPA production even in the absence of heat shock or other obvious stress. After heat shock (42 degrees C, 2 hr), there was up to a 40-fold increase in tPA production. Production of tPA protein occurred within the first 5 h after the heat shock and was due to a burst of hsp70/tPA transcription during the heat shock. The hsp70/tPA transcripts appeared to have a short half-life. Thus, stable mouse cell lines carrying hsp70/tPA hybrid genes can be induced by a short heat shock to transcribe high levels of hsp70/tPA mRNAs and, subsequently, to produce elevated levels of tPA protein.
authors.affiliation: Celltech Limited, Slough, Berkshire, UK.
authors.forename: M M
authors.initials: MM
authors.lastname: Bendig
authors.forename: P E
authors.initials: PE
authors.lastname: Stephens
authors.forename: M I
authors.initials: MI
authors.lastname: Cockett
authors.forename: C C
authors.initials: CC
authors.lastname: Hentschel
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1; D002460:Cell Line; D002472:Cell Transformation, Viral; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D006360:Heat-Shock Proteins; D011506:Proteins; D012333:RNA, Messenger; D010959:Tissue Plasminogen Activator