Effect of superoxide and lipid peroxide on cytosolic free calcium concentration in cultured pig aortic endothelial cells.
The effect of reactive oxygen on cytosolic free calcium concentration [( Ca++]i) in pig aortic endothelial cells (ECs) was studied. Linoleate hydroperoxide (LHO) and superoxide radicals generated from xanthine with xanthine oxidase (X-XO) were used as sources of reactive oxygen. [Ca++]i in ECs was measured with quin 2 and the value for quiescent ECs was 112 +/- 11 nM. Both LHO and X-XO increased [Ca++]i in a dose-dependent manner without accompanying the significant cellular damage. Nifedipine suppressed the increase in [Ca++]i provoked by LHO and X-XO. Thus, the biological effects of reactive oxygen might be mediated, at least in part, by the activation of voltage-dependent calcium channels in ECs.
Hirosumi
J
J
Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
Ouchi
Y
Y
Watanabe
M
M
Kusunoki
J
J
Nakamura
T
T
Orimo
H
H
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008041:Linoleic Acids / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000494:pharmacology*; D013481:Superoxides / Q000494:pharmacology*; D013552:Swine; D014969:Xanthine Oxidase / Q000378:metabolism
D008041:Linoleic Acids; D008054:Lipid Peroxides; D013481:Superoxides; C008285:linoleic acid hydroperoxide; D014969:Xanthine Oxidase; D002118:Calcium