Type I-like intervening sequences are found in the rDNA of the nematode Ascaris lumbricoides.
The intervening sequences in the large ribosomal RNA gene of Ascaris lumbricoides var. suum show many similarities to the type I insertions, previously found only in some insect species. They include structural features, but also a presumed transcriptional inactivity in vivo: No transcript of the rDNA intervening sequence in A. lumbricoides could be detected in Northern and dot blot hybridizations. However, the primary structure of the Pol I promoter region is well conserved in interrupted and uninterrupted genes. Moreover, genes with an intervening sequence are correctly initiated in a whole-cell in vitro extract from Ascaris oogonia. Hence, the presence of the intervening sequence alone does not seem to account for a transcriptional inhibition in rRNA genes. As with the type I insertions of insect rDNA, some copies of the A. lumbricoides intervening sequence are also present in locations outside the rDNA cluster. About 50% of the extraribosomal copies are found in a repetitive sequence of the genome, and additional copies are inserted in unique sequences. These striking analogies to type I insertions are discussed, and lead to the conclusion that the two phenomena are undoubtedly related. This is the first report proving the presence of a type I-like insertion element outside of the class Insecta.
Neuhaus
H
H
Institute of Zoology, University of Fribourg, Switzerland.
Müller
F
F
Etter
A
A
Tobler
H
H
D000818:Animals; D001200:Ascaris / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements; D004275:DNA, Ribosomal / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics; D005796:Genes; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal