High performance liquid chromatographic determination of cholecalciferol (vitamin D3) in food--a comparison with a bioassay method.
The vitamin D3 levels in fortified milk (0.5% fat) and other low fat products, such as infant formulas, porridges and gruels were determined chemically and biologically. The chemical method was based on a semi-preparative straight phase clean-up procedure, followed by an analytical reversed phase quantifying step. The biological tests were carried out using the curative x-ray method. The agreement between the results of the two method was good. The overall recoveries for the HPLC-method were 95% (SD = 2.8%, n = 13) at concentrations of 10 micrograms per 100 g and 5 micrograms per litre for fortified products and milk, respectively. The minimum detectable amount was 250 pg vitamin D3. The pre-vitamin D3 formation was studied during saponification conditions and the isomer ratio was estimated.
Johnsson
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Swedish National Food Administration, Uppsala.
Hessel
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D000818:Animals; D001681:Biological Assay; D002762:Cholecalciferol / Q000008:administration & dosage / Q000032:analysis* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D006358:Hot Temperature; D006878:Hydroxides; D007225:Infant Food / Q000032:analysis; D008892:Milk / Q000032:analysis; D011188:Potassium; D017680:Potassium Compounds; D051381:Rats; D012279:Rickets / Q000188:drug therapy
D006878:Hydroxides; D017680:Potassium Compounds; D002762:Cholecalciferol; D011188:Potassium; C029943:potassium hydroxide