title: Modification of the leader protein (Lb) of foot-and-mouth disease virus.
abstract: Translation of foot-and-mouth disease virus RNA for extended periods in rabbit reticulocyte lysates results in the appearance of a previously undescribed protein. A protein with similar properties can also be detected in BHK cells at late times after virus infection. Specific immunoprecipitation has shown that this protein (Lb') is closely related to the smaller of the two leader proteins, Lb, although it migrates with an apparently higher Mr in SDS-polyacrylamide gels. The conversion of Lb to Lb' can be mimicked by treatment with carboxypeptidase B. It is suggested that C-terminal trimming of Lb to produce Lb' results in an increase in negative charge and is responsible for its slower migration in SDS-PAGE.
authors.affiliation: Department of Virology, Wellcome Biotechnology Limited, Beckenham, U.K.
authors.forename: D V
authors.initials: DV
authors.lastname: Sangar
authors.forename: R P
authors.initials: RP
authors.lastname: Clark
authors.forename: A R
authors.initials: AR
authors.lastname: Carroll
authors.forename: D J
authors.initials: DJ
authors.lastname: Rowlands
authors.forename: B E
authors.initials: BE
authors.lastname: Clarke
mesh_terms: D005537:Aphthovirus / Q000378:metabolism*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D013997:Time Factors; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D014764:Viral Proteins; D010447:Peptide Hydrolases