Regional brain cholinesterase activity in rats injected intraperitoneally with anatoxin-a(s) or paraoxon.
Adult male Long-Evans rats were injected intraperitoneally with 1.5, 3.0 or 9.0 micrograms/kg of anatoxin-a(s) that had been extracted from laboratory-grown Anabaena flos-aquae NRC-525-17, 800 micrograms/kg of paraoxon, or a control solution. Blood, anterior spinal cord, and brain cerebellar, cortical, medullary, midbrain, hippocampal, hypothalamic, olfactory and striatal cholinesterase activity was determined in rats that died prior to 2 hours or were anesthetized and killed at 2 hours. Unlike paraoxon, anatoxin-a(s) did not cause detectable inhibition of cholinesterase in the central nervous system, but did cause inhibition of cholinesterase in blood, suggesting that anatoxin-a(s) is strictly a peripheral cholinesterase inhibitor.
Cook
W O
WO
Department of Veterinary Biosciences, University of Illinois, Urbana 61801.
Dellinger
J A
JA
Singh
S S
SS
Dahlem
A M
AM
Carmichael
W W
WW
Beasley
V R
VR
D000818:Animals; D001427:Bacterial Toxins; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002800:Cholinesterase Inhibitors; D002802:Cholinesterases / Q000097:blood / Q000378:metabolism*; D000087522:Cyanobacteria Toxins; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D008387:Marine Toxins / Q000008:administration & dosage / Q000633:toxicity*; D052998:Microcystins; D010261:Paraoxon / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D013116:Spinal Cord / Q000187:drug effects* / Q000201:enzymology; D014326:Tropanes
D001427:Bacterial Toxins; D002800:Cholinesterase Inhibitors; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D014326:Tropanes; C509783:anatoxin a; D002802:Cholinesterases; D010261:Paraoxon