Cloning and characterization of the pH 2.5 acid phosphatase gene, appA: cyclic AMP mediated negative regulation.
The structural gene for an acid phosphatase coded for by the gene appA of Escherichia coli K12 was cloned from a cosmid library into pBR322 and the restriction map determined. Several appA deletion plasmids and a smaller appA+ plasmid were constructed by in vitro recombination techniques and tested for their ability to complement an appA1 mutation. The appA gene was localized within a 2.1 kb segment. Its orientation was determined by construction of a hybrid plasmid carrying an appA-lacZ fusion. beta-galactosidase synthesized from the appA promoter was negatively regulated by cyclic AMP.
Touati
E
E
Régulation de l'expression génétique, Institut Pasteur, Paris, France.
Danchin
A
A
D000135:Acid Phosphatase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D006863:Hydrogen-Ion Concentration; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D004262:DNA Restriction Enzymes; D000135:Acid Phosphatase; D001616:beta-Galactosidase