title: Isolation of cathepsin D using three-phase partitioning in t-butanol/water/ammonium sulfate.
abstract: A 6-h procedure for the isolation of bovine cathepsin D is described. The procedure involves essentially only two steps; three-phase partitioning in t-butanol/water/ammonium sulfate followed by affinity chromatography on pepstatin-agarose. The major advantage of this new method over previous methods is the greatly reduced time required to obtain comparably pure cathepsin D.
authors.affiliation: Department of Biochemistry, University of Natal, Pietermaritzburg, South Africa.
authors.forename: G R
authors.initials: GR
authors.lastname: Jacobs
authors.forename: R N
authors.initials: RN
authors.lastname: Pike
authors.forename: C
authors.initials: C
authors.lastname: Dennison
mesh_terms: D000645:Ammonium Sulfate; D000818:Animals; D000440:Butanols; D002402:Cathepsin D / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D013154:Spleen / Q000201:enzymology; D014867:Water; D020002:tert-Butyl Alcohol
chemical_list: D000440:Butanols; D014867:Water; D002402:Cathepsin D; D020002:tert-Butyl Alcohol; D000645:Ammonium Sulfate