title: Analysis and isolation of cytomegalovirus DNA by field inversion gel electrophoresis.
abstract: High molecular weight human cytomegalovirus (CMV) DNA was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. A high yield of CMV DNA molecules was obtained within 1 week of infecting the cell culture. Labelling of the viral DNA with biotin by nick translation enabled the detection of CMV-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.
authors.affiliation: Department of Pathology, University of Amsterdam, The Netherlands.
authors.forename: F M
authors.initials: FM
authors.lastname: van den Berg
authors.forename: M
authors.initials: M
authors.lastname: Jiwa
authors.forename: R
authors.initials: R
authors.lastname: Rook
authors.forename: J L
authors.initials: JL
authors.lastname: Geelen
mesh_terms: D000311:Adrenal Glands / Q000382:microbiology; D003587:Cytomegalovirus / Q000032:analysis* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000382:microbiology; D004279:DNA, Viral / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D009693:Nucleic Acid Hybridization
chemical_list: D004279:DNA, Viral