title: Phosphorylation of phosphatidylinositol associated with the nicotinic acetylcholine receptor of Torpedo californica.
abstract: When isolated, detergent solubilized and affinity chromatographically purified nicotinic acetylcholine receptor of Torpedo californica electric organ is incubated with [gamma-32P]ATP/Mg2+, phosphatidylinositol 4-phosphate (PIP) is formed from receptor associated phosphatidylinositol (PI). This receptor associated endogenous kinase activity is enhanced by orthovanadate and, remarkably, also by acetylcholine. Exogenously added PI-kinase only increases the phosphorylation rate if vanadate is present. PIP as the main phosphorylation product (up to 95%) remains bound to the beta-, gamma- and delta-subunits of the receptor and to the receptor associated v-protein. The alpha-subunits do not carry 32p phosphate; no phosphatidylinositol 4,5-bisphosphate formation has been observed. Concomitant to lipid phosphorylation tyrosine and serine residues are phosphorylated (5% of total incorporated 32P phosphate).
authors.affiliation: Physikalische und Biophysikalische Chemie, Fakultät für Chemie, Universität Bielefeld, West Germany.
authors.forename: R
authors.initials: R
authors.lastname: Kiehl
authors.forename: M
authors.initials: M
authors.lastname: Varsányi
authors.forename: E
authors.initials: E
authors.lastname: Neumann
mesh_terms: D019870:1-Phosphatidylinositol 4-Kinase; D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014101:Torpedo
chemical_list: D010716:Phosphatidylinositols; D011978:Receptors, Nicotinic; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase; D000109:Acetylcholine