An improved method for the isolation of islets from the rat pancreas.
The conventional method for rat islet isolation was improved by injection of collagenase solution (1.3 mg/ml) into the bile duct and the concomitant dissection of the inferior vena cava, static incubation of the removed distended pancreas for 15 min at 37 degrees C and filtration of the digested tissue through two different meshes of 1190 and 590 micron in pore size. This new technique led to about 2.5 times higher yield of viable islets than the conventional method.
Hara
Y
Y
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
Taniguchi
H
H
Yamashiro
Y
Y
Kuroda
N
N
Ishihara
K
K
Ejiri
K
K
Baba
S
S
D000818:Animals; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D003012:Microbial Collagenase / Q000378:metabolism / Q000494:pharmacology; D010179:Pancreas / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D003012:Microbial Collagenase