title: Characterization and mapping of equine herpesvirus type 1 immediate early, early, and late transcripts.
abstract: Northern blot analysis was used to characterize and map equine herpesvirus type 1 (EHV-1) immediate early (IE), early, and late transcripts. Genomic EHV-1 DNA and cloned EHV-1 restriction endonuclease fragments, representing the entire genome, were 32P-labeled and hybridized to immobilized total cell RNA isolated from EHV-1 infected rabbit kidney cells incubated in the presence or absence of metabolic inhibitors. A single 6.0 kilobase (kb) IE transcript mapped to viral inverted repeat sequences. Approximately 41-45 early transcripts ranging in size from 0.8 to 6.4 kb and 18-20 late transcripts ranging in size from 0.8 to 10.0 kb were identified. These findings demonstrate that EHV-1 gene expression is regulated at the level of transcription, although regulation at the level of translation is also possible. The results provide a basis for examining alterations in viral gene expression in EHV-1 oncogenically transformed and persistently infected cells.
authors.affiliation: Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130.
authors.forename: W L
authors.initials: WL
authors.lastname: Gray
authors.forename: R P
authors.initials: RP
authors.lastname: Baumann
authors.forename: A T
authors.initials: AT
authors.lastname: Robertson
authors.forename: D J
authors.initials: DJ
authors.lastname: O'Callaghan
authors.forename: J
authors.initials: J
authors.lastname: Staczek
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D004262:DNA Restriction Enzymes