[Comparative restriction analysis of chromosomal DNA of strains of Photobacterium leiognathi].
Chromosomal DNA in 5 hereditary variants occurring in Photobacterium leiognathi population was subjected to restriction analysis. The variants differed in the levels and regulation of luminescence and colony morphology. Agarose electrophoresis of DNA fragments isolated after exposure to Hind II, Bam HI, Bgl I and Pst I restriction endonucleases revealed respectively 38, 28, 35 and 29 fragments equally distributed by their molecular weights. Electrophoregrams of the 5 strains were absolutely identical. After exposure of DNA of all the strains to PVu II, Xho II, Sal GI and Eco RI restriction endonucleases there were detected no fragments. The pleoiotropic genetic variation in these strains was not associated with large deletions or amplification of chromosomal DNA regions.
Videlets
I Iu
IIu
Starodubtseva
L I
LI
Podgornova
G P
GP
Lutskaia
N I
NI
Shenderov
A N
AN
D002876:Chromosomes, Bacterial / Q000187:drug effects* / Q000648:ultrastructure; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000187:drug effects* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation / Q000187:drug effects; D008163:Luminescent Measurements; D010641:Phenotype; D010776:Photobacterium / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes
Sravnitel'nyĭ restriktsionnyĭ analiz khromosomnoĭ DNK shtammov Photobacterium leiognathi.