Purification of cellular retinaldehyde-binding protein from bovine retina and retinal pigment epithelium.
Cellular retinaldehyde-binding protein (CRALP) has been purified from extracts of bovine retina or retinal pigment epithelium by a procedure employing an initial, high-capacity anion exchange chromatographic step and anion exchange HPLC for removal of a persistent contaminant. The procedure also yields fractions containing three other retinoid-binding proteins present in retina (cellular retinol-, cellular retinoic acid- and interphotoreceptor retinol-binding proteins; CRBP, CRABP and IRBP, respectively). Procedures are described for labeling CRALBP with 9-cis-retinaldehyde, 11-cis-retinaldehyde, or 11-cis-retinol. There are approx. 3 nmol of CRALBP per adult bovine eye and the binding protein is ca. 0.5% of the soluble protein of a retinal supernatant.
Saari
J C
JC
Department of Ophthalmology, University of Washington, School of Medicine, Seattle 98195.
Bredberg
D L
DL
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005136:Eye Proteins; D010857:Pigment Epithelium of Eye / Q000032:analysis*; D011485:Protein Binding; D018168:Receptors, Retinoic Acid; D012160:Retina / Q000032:analysis*; D012172:Retinaldehyde / Q000302:isolation & purification*; D012176:Retinoids / Q000302:isolation & purification*; D012177:Retinol-Binding Proteins / Q000302:isolation & purification; D054840:Retinol-Binding Proteins, Cellular; D000099214:Retinol-Binding Proteins, Interstitial
D002352:Carrier Proteins; D005136:Eye Proteins; D018168:Receptors, Retinoic Acid; D012172:Retinaldehyde; D012176:Retinoids; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D000099214:Retinol-Binding Proteins, Interstitial; C020932:11-cis-retinal-binding protein