Comparative effects of two sulfhydryl reagents on the activities of a multifunctional red cell enzyme: bisphosphoglycerate mutase.
The effects of two sulfhydryl reagents on the three activities of bisphosphoglycerate mutase have been compared. Under N-ethylmaleimide treatment all the activities were inhibited except for 60% of the non-stimulated phosphatase. With iodoacetamide the mutase and the stimulated-phosphatase activities were completely inhibited whereas the non-stimulated phosphatase and 60% of the synthase activities were unaffected. 2,3-bisphosphoglycerate protected all the activities of the enzyme against inactivation by the two sulfhydryl reagents whereas 3-phosphoglycerate protected them only against iodoacetamide. 2-phosphoglycolate had an identical effect to that of 3-phosphoglycerate except for its effect on the non-stimulated phosphatase activity, which was slightly enhanced under N-ethylmaleimide treatment.
Rosa
R
R
INSERM U.91, Hôpital Henri Mondor, Créteil, France.
Calvin
M C
MC
D019794:2,3-Diphosphoglycerate; D001731:Bisphosphoglycerate Mutase / Q000097:blood*; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology*; D005033:Ethylmaleimide / Q000494:pharmacology*; D005988:Glyceric Acids / Q000378:metabolism; D006801:Humans; D007460:Iodoacetamide / Q000494:pharmacology*; D007461:Iodoacetates / Q000494:pharmacology*; D010770:Phosphotransferases / Q000097:blood*
D004163:Diphosphoglyceric Acids; D005988:Glyceric Acids; D007461:Iodoacetates; D019794:2,3-Diphosphoglycerate; C005156:3-phosphoglycerate; D010770:Phosphotransferases; D001731:Bisphosphoglycerate Mutase; D005033:Ethylmaleimide; D007460:Iodoacetamide