Reduction of dye coupling in glial cultures by microinjection of antibodies against the liver gap junction polypeptide.
Intracellular injection of antibodies to the 27-kDa liver gap junction polypeptide have been shown previously to uncouple pairs of cultured mammalian hepatocytes, cardiac myocytes, and ganglionic neurons (Proc. Natl. Acad. Sci. U.S.A., 82 (1985) 2412-2416). In confluent primary cultures of astrocytes, similar injections significantly reduced dye coupling for cells closer than 80 micron to the injected glial cell. Western blots identified a 27-kDa protein in extracts of the astrocyte cultures that cross-react with the gap junction-specific antibodies. These results suggest that homologous gap junction polypeptides exist in liver and glial cells.
Dudek
F E
FE
Department of Physiology, Tulane University School of Medicine, New Orleans, LA 70112.
Gribkoff
V K
VK
Olson
J E
JE
Hertzberg
E L
EL
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D001921:Brain / Q000502:physiology*; D002478:Cells, Cultured; D017630:Connexins; D003429:Cross Reactions; D005456:Fluorescent Dyes; D007546:Isoquinolines; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000276:immunology / Q000502:physiology*; D008845:Microinjections; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D017630:Connexins; D005456:Fluorescent Dyes; D007546:Isoquinolines; D008565:Membrane Proteins; C017475:lucifer yellow