Detection of cytopathic and noncytopathic bovine viral diarrhea virus in cell culture with an immunoperoxidase test.
Bovine viral diarrhea virus (BVDV) antigen was detected in cell culture with an indirect immunoperoxidase (IP) procedure using a specific monoclonal antibody, and an avidin-biotin-peroxidase complex. Cytopathic and noncytopathic strains of the virus showed similar patterns of IP staining until 3 days post-infection. At six days post-infection, intensity of staining decreased in cell cultures infected with noncytopathic virus, but not with cytopathic virus. The IP procedure detected BVDV antigen in cells used to isolate virus from tissues of aborted bovine fetuses and peripheral blood lymphocytes of adult cattle. Immunoperoxidase detected BVDV isolates from 10 of 44 cases of abortion of which seven of these were noncytopathic. Noncytopathic BVDV isolates from the peripheral blood lymphocytes of 7 of 65 animals were identified.
Smith
G H
GH
Diagnostic Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
Collins
J K
JK
Carman
J
J
Minocha
H C
HC
D000034:Abortion, Veterinary / Q000276:immunology / Q000382:microbiology; D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis; D002417:Cattle; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D007124:Immunoenzyme Techniques; D010576:Pestivirus / Q000302:isolation & purification*; D011247:Pregnancy
D000956:Antigens, Viral