Cloning and characterization of the metallothionein-I gene from mouse LMTK cells.
A clone of about 14 kb containing the metallothionein MT-I gene and three repetitive sequences, was isolated from a genomic library of mouse LMTK DNA. The MT-I gene was functional. Transfected cells became cadmium resistant. Two of the three repetitive sequences were moderately repetitive while the other was closely related to the R family.
Brzezinski
R
R
Département de Microbiologie, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
Smorawinska
M
M
Vézina
G
G
Thibodeau
J
J
Thirion
J P
JP
D000818:Animals; D002104:Cadmium / Q000633:toxicity; D019256:Cadmium Chloride; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D009710:Nucleotide Mapping
D002104:Cadmium; D008668:Metallothionein; D004262:DNA Restriction Enzymes; D019256:Cadmium Chloride