Methods for DNA extraction from Candida albicans.
Three different methods are described for the extraction of total genomic DNA from the dimorphic fungus Candida albicans. One method, which enables a large number of cultures to be processed simultaneously, involves pulverizing dried cells with glass beads and then allowing the disrupted cells to break apart, autolyse, by incubation in a solution which includes sorbitol and a nonionic detergent. DNA extraction by a second method with a French pressure cell can be utilized on cultures in any phase of growth, but is not practical for processing numerous samples. The third method, which involves induction of spheroplasts, is commonly used for DNA extraction from various yeasts but is not suited for processing many samples simultaneously. The DNA extracted with the three procedures is comparable in quality; in particular, it is of high molecular size (greater than 30 kbp) and reacts readily with DNA-modifying enzymes such as restriction endonucleases.
Glee
P M
PM
Department of Microbiology, Montana State University, Bozeman 59717.
Russell
P J
PJ
Welsch
J A
JA
Pratt
J C
JC
Cutler
J E
JE
D002176:Candida albicans / Q000235:genetics*; D002458:Cell Fractionation; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004271:DNA, Fungal / Q000302:isolation & purification* / Q000378:metabolism; D005898:Glass; D008863:Microspheres; D011312:Pressure; D013104:Spheroplasts / Q000032:analysis
D004271:DNA, Fungal; D004262:DNA Restriction Enzymes