Detection of tumor-associated membrane proteins in prostate and bladder carcinomas by means of protein blotting.
Analysis of membrane proteins by Western blotting has revealed both overexpression of proteins of molecular weight 10-200 kD (in particular, of proteins of MW less than 43 kD) and increased glycosylation in a xenografted human small cell undifferentiated prostatic carcinoma, and in two xenografted human bladder tumor cell lines compared with preparations from normal human tissue. Of potential functional significance were: a) a 43 kD protein in the bladder line, UCRU-BL-13, which demonstrated increased synthesis and a marked increase in the degree of glycosylation, and b), a 28 kD ConA-binding protein in prostatic tissue which was absent in normal tissue, present in intermediate quantity in a benign hyperplasia and greatly overexpressed in small cell carcinoma. This study demonstrates the utility of the protein blotting/autoradiography technique for the investigation of tumor membrane proteins.
Ford
S A
SA
Urological Cancer Research Unit, Royal Prince Alfred Hospital, Sydney, Australia.
Russell
P J
PJ
Jelbart
M
M
Raghavan
D
D
D001345:Autoradiography; D015153:Blotting, Western; D002295:Carcinoma, Transitional Cell / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*
D008565:Membrane Proteins; D009363:Neoplasm Proteins