Genetics and biochemistry of collagen binding-triggered glandular differentiation in a human colon carcinoma cell line.
We have examined the interaction between collagen binding and epithelial differentiation by using a human colon carcinoma cell line (SW1222) that can differentiate structurally when grown in a three-dimensional collagen gel to form glandular structures. As much as 66% inhibition of glandular differentiation can be achieved by addition to the culture of a synthetic peptide (2 mg/ml) containing the Arg-Gly-Asp-Thr (RGDT) sequence, which is a cell recognition site found in collagen. Arg-Gly-Asp-Thr also inhibited the cell attachment to collagen-coated plates. A control peptide containing the Arg-Gly-Glu-Thr (RGET) sequence had no effect on cell adhesion or cell differentiation. Chromosome 15 was found in all human-mouse hybrid clones [from a cross between SW1222 and a mouse rectal carcinoma cell line (CMT-93)] that could differentiate in the collagen gel and bind collagen. Both binding to collagen and glandular differentiation of the hybrid cells were also inhibited by Arg-Gly-Asp-Thr as for the parent cell line SW1222. The ability of SW1222 cells to express the differentiated phenotype appears, therefore, to be determined by an Arg-Gly-Asp-directed collagen receptor on the cell surface that is controlled by a gene on chromosome 15.
Pignatelli
M
M
Director's Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Bodmer
W F
WF
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002884:Chromosomes, Human, Pair 15; D003094:Collagen / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D006822:Hybrid Cells; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D038981:Receptors, Collagen; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology*
D009842:Oligopeptides; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; C047981:arginyl-glycyl-aspartic acid; D003094:Collagen