title: Antigonadal activity of the neurohypophysial hormones: in vivo regulation of testicular function of hypophysectomized rats.
abstract: The "antigonadal" potential of the neurohypophysial hormones, previously demonstrated in vitro, was evaluated in vivo using hypophysectomized male rats. This approach minimizes the likelihood that the in vivo "antigonadal" effect of the neurohypophysial hormones may be due to their ability to attenuate the release of pituitary gonadotropins. Given that the identity of the putative endogenous occupant of testicular pressor-selective neurohypophysial receptors remains uncertain, use was made of a substitute probe, arginine vasotocin (AVT), the utility of which has been demonstrated in vitro. Concurrent in vivo treatment of follicle-stimulating hormone (FSH; 5 micrograms/rat/day)-maintained immature hypophysectomized rats with increasing doses of AVT (0.25-25 microgram/rat/day) produced significant (P less than 0.05) dose-dependent inhibition of the testicular luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor binding capacity (but not affinity; Kd = 1.8 X 10(-10) M) from 8.8 +/- (standard error; SE) 0.4 ng/testis to a level (3.2 +/- 0.2 ng/testis) lower than that of controls (64% reduction). This AVT-induced decrease in the testicular LH/hCG receptor content of FSH-maintained immature hypophysectomized rats was associated with significant (P less than 0.05) decrements in the hCG- and N6, 2'-O-dibutyryladeosine cyclic 3',5'-monophosphate [( Bu]2cAMP)-stimulated accumulation of 3 alpha-hydroxy-5 alpha-androstan-17-one (androsterone; 52% and 42% inhibition, respectively), with virtual elimination (98% inhibition) of the forskolin-stimulated accumulation of extracellular cAMP by testicular incubates in vitro, as well as with profound suppression of spermatogenesis. Taken together, these observations indicate that the "antigonadal" effect of the neurohypophysial hormones previously demonstrated in vitro, can be fully reproduced in vivo, and that the "antigonadal" activity of the neurohypophysial hormones may be accounted for, in large part, by decreased testicular LH/hCG binding capacity, stimulable adenylate cyclase activity, and cAMP-supported androgen biosynthesis.
authors.affiliation: Department of Obstetrics/Gynecology, University of Maryland School of Medicine, Baltimore 21201.
authors.forename: E Y
authors.initials: EY
authors.lastname: Adashi
authors.forename: C E
authors.initials: CE
authors.lastname: Resnick
authors.forename: B R
authors.initials: BR
authors.lastname: Zirkin
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000728:Androgens / Q000096:biosynthesis; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007016:Hypophysectomy; D008297:Male; D009924:Organ Culture Techniques; D010121:Oxytocin / Q000494:pharmacology; D051381:Rats; D011974:Receptors, LH / Q000032:analysis; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000032:analysis / Q000187:drug effects* / Q000502:physiology; D014668:Vasotocin / Q000494:pharmacology*
chemical_list: D000728:Androgens; D011974:Receptors, LH; D005576:Colforsin; D010121:Oxytocin; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D014668:Vasotocin