Astatination of proteins using an N-succinimidyl tri-n-butylstannyl benzoate intermediate.
A method is described for labeling proteins with 7.2 h half-life 211 At. The alpha particle-emitting nuclide was coupled to goat IgG using an N-succinimidyl 3-(tri-n-butylstannyl) benzoate intermediate. The reaction and purification sequence requires about 2 h to produce 211 At-labeled IgG in 25-40% radiochemical yield. Comparative blood clearance measurements in mice suggest that the 211 At-labeled IgG conjugate is stable in vivo.
Zalutsky
M R
MR
Department of Radiology, Duke University Medical Center, Durham, NC 27710.
Narula
A S
AS
D000818:Animals; D001246:Astatine; D001565:Benzoates; D004355:Drug Stability; D005260:Female; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods*; D051379:Mice; D011506:Proteins; D014220:Trialkyltin Compounds
D001565:Benzoates; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011506:Proteins; D014220:Trialkyltin Compounds; C055156:N-succinimidyl-3-(tri-n-butylstannyl)benzoate; D001246:Astatine