Protection of mice from lethal infection with Aujeszky's disease virus by immunization with purified gVI.
Virus envelope glycoprotein gVI (gp50) of Aujeszky's disease virus (ADV) was purified from a Nonidet P40-solubilized lysate of ADV-infected Vero cells by immunoaffinity chromatography using a monoclonal antibody against gVI. Mice immunized by the purified gVI produced virus-neutralizing antibody and were successfully protected against subsequent challenge with ADV.
Ishii
H
H
Ghen Corporation, Gifu Laboratory, Japan.
Kobayashi
Y
Y
Kuroki
M
M
Kodama
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002846:Chromatography, Affinity; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007114:Immunization; D007118:Immunoassay; D051379:Mice; D008807:Mice, Inbred BALB C; D011557:Pseudorabies / Q000517:prevention & control*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification; D014765:Viral Vaccines
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C042966:pseudorabies virus glycoproteins