title: Irreproducibility of neutralization of herpes simplex virus under conditions where antibody is not in excess.
abstract: Neutralizing activity against herpes simplex virus was significantly reduced if initial virus titers were greater than 10(6) PFU/ml; there was no significant neutralization when initial virus titers approached 10(8) PFU/ml. This was a result of utilization of all available antibody by virus particles and 'free' virus antigen and emphasizes the importance of conducting virus neutralization tests under conditions of antibody excess.
authors.affiliation: Department of Medical Microbiology, University of Birmingham, UK.
authors.forename: D
authors.initials: D
authors.lastname: Shariff
authors.forename: J A
authors.initials: JA
authors.lastname: Hallworth
authors.forename: A
authors.initials: A
authors.lastname: Buchan
authors.forename: G R
authors.initials: GR
authors.lastname: Skinner
mesh_terms: D000914:Antibodies, Viral / Q000008:administration & dosage; D000956:Antigens, Viral / Q000008:administration & dosage; D009500:Neutralization Tests / Q000379:methods*; D018139:Simplexvirus / Q000145:classification / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral