Cross-protection of mu and delta opioid receptors in the mouse vas deferens.
Opioid receptors in the C57/BL mouse vas deferens can be irreversibly blocked by pre-treatment with beta-chlornaltrexamine. Pre-incubation with the highly selective delta-receptor antagonist ICI 174864 concentration-dependently protected delta-receptors from inactivation, but the same concentrations similarly protected mu receptors. Pre-incubation with selective mu-receptor agonist, [D-Ala2, MePhe4, Gly(ol)5]enkephalin, also protected both mu and delta receptors to the same degree. Neither ligand protected k-opioid receptors. The results suggest that there may be a structural interaction between mu and delta receptors in the mouse vas deferens.
Sheehan
M J
MJ
Department of Neuropharmacology, Glaxo Group Research Ltd., Ware, Herts., U.K.
Hayes
A G
AG
Tyers
M B
MB
D000818:Animals; D005260:Female; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009271:Naltrexone / Q000031:analogs & derivatives* / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology*; D011957:Receptors, Opioid / Q000187:drug effects* / Q000378:metabolism; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D014649:Vas Deferens / Q000378:metabolism*
D009292:Narcotic Antagonists; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D009271:Naltrexone; C017120:chlornaltrexamine