Effects of carbamylcholine on cyclic nucleotide-dependent protein kinase activity in corneal epithelium during resurfacing.
Nuclear and total cellular cyclic nucleotide-dependent protein kinase activity was assayed in corneal epithelium from carbamylcholine-treated and control eyes of rabbits with and without resurfacing acid burn defects. In epithelium from resurfacing corneas carbamylcholine significantly elevated both nuclear and total cellular cGMP-dependent protein kinase activity and reduced activity of cAMP-dependent protein kinase. The drug also increased the proportion of total cellular cGMP kinase activity represented by nuclear activity, suggesting that the drug may enhance nuclear translocation of cGMP-dependent protein kinase.
Colley
A M
AM
Department of Ophthalmology, Emory University School of Medicine, Emory Eye Center, Atlanta, GA 30322.
Law
M L
ML
D000287:Administration, Topical; D000818:Animals; D002057:Burns, Chemical / Q000473:pathology*; D002217:Carbachol / Q000008:administration & dosage*; D003315:Cornea / Q000187:drug effects*; D065306:Corneal Injuries; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects; D005126:Eye Burns / Q000139:chemically induced*; D008297:Male; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects
D002217:Carbachol; D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP