title: Catecholamine effect on cyclic adenosine 3':5'-monophosphate level in isolated catfish hepatocytes.
abstract: The effect of catecholamines (epinephrine, norepinephrine, isoproterenol, and phenylephrine) on cyclic adenosine 3':5'-monophosphate (cAMP) level in isolated catfish (Ictalurus melas) liver cells was studied in the presence or absence of alpha (phentolamine) and beta (propranolol)-receptor antagonists. All catecholamines increased the hepatocyte cAMP level: the rank of their potency was epinephrine = isoproterenol greater than norepinephrine greater than phenylephrine. Propranolol completely blocked the catecholamine effect; phentolamine was ineffective. Results confirm previous findings (L. Brighenti, A. C. Puviani, M. E. Gavioli, and C. Ottolenghi, 1987, Gen. Comp. Endocrinol. 66, 306-313) that epinephrine and norepinephrine act via beta-receptor activation. However, the comparison of the effects of isoproterenol and phenylephrine on cAMP with those on phosphorylase alpha and on glycogen breakdown suggests that a more complex mechanism is possibly involved in the catecholamine effect on catfish glycogenolysis.
authors.affiliation: Institute of General Physiology, University of Ferrara, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Brighenti
authors.forename: A C
authors.initials: AC
authors.lastname: Puviani
authors.forename: M E
authors.initials: ME
authors.lastname: Gavioli
authors.forename: E
authors.initials: E
authors.lastname: Fabbri
authors.forename: C
authors.initials: C
authors.lastname: Ottolenghi
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000494:pharmacology*; D002397:Catfishes; D000242:Cyclic AMP / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology
chemical_list: D002395:Catecholamines; D011433:Propranolol; D000242:Cyclic AMP; D010646:Phentolamine