[A method for S-adenosylmethionine determination in extracts of microbial cells].
A technique for quantification of S-adenosylmethionine in microbial cell-free extracts is proposed that involves dilution of S-adenosyl-L-(methyl-3H)methionine with non-labelled S-adenosylmethionine followed by DNA-cytosine-methyltransferase reaction. The content of S-adenosylmethionine and the activity of S-adenosylmethionine synthetase in yeasts and E. coli MRE-600 are in good agreement with the results obtained with labelled L-methionine and consistent with literature data. The sensitivity of the technique is about 0.1 nmol/0.1 ml of the reaction mixture (10(-6) M). The error was about 5% in every series of experiments. However, the combined use of different DNA-methyltransferase preparations resulted in a higher experimental error (up to 15%), which should be taken into consideration.
Bykovskaia
S V
SV
Sysoev
O V
OV
Bur'ianov
Ia I
IaI
D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000032:analysis*; D008722:Methods; D010843:Pichia / Q000032:analysis*; D012436:S-Adenosylmethionine / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D004718:Saccharomycetales / Q000032:analysis*
D012436:S-Adenosylmethionine; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Metod opredeleniia S-adenozilmetionina v ékstraktakh kletok mikroorganizmov.