Alpha-2-adrenergic modulation of the parathyroid hormone-inhibition of phosphate uptake in cultured renal (OK) cells.
Parathyroid hormone enhances the formation of cAMP and decreases the Na+-dependent uptake of phosphate in cultured renal cells derived from the American opossum (OK cells). Epinephrine, acting as an alpha 2-adrenergic agonist, inhibits the PTH-induced synthesis of cAMP by a pertussis toxin-sensitive mechanism and blunts the inhibition of phosphate transport by PTH. Na+-dependent alpha-methylglucoside and Na+ uptakes by the cells are unaffected by PTH and epinephrine. These findings suggest that alpha 2-adrenergic agonists may selectively modulate PTH-sensitive phosphate transport in the renal proximal tubule.
Cheng
L
L
Laboratory of Biological Chemistry, National Institute on Aging, Baltimore, MD 21224.
Liang
C T
CT
Precht
P
P
Sacktor
B
B
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D004837:Epinephrine / Q000378:metabolism / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D009893:Opossums; D010281:Parathyroid Hormone / Q000494:pharmacology*; D037342:Pertussis Toxin; D010710:Phosphates / Q000378:metabolism*; D011224:Prazosin; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000502:physiology; D012964:Sodium / Q000502:physiology; D010566:Virulence Factors, Bordetella; D015016:Yohimbine
D010281:Parathyroid Hormone; D010710:Phosphates; D011942:Receptors, Adrenergic, alpha; D010566:Virulence Factors, Bordetella; D015016:Yohimbine; D012964:Sodium; D000242:Cyclic AMP; D037342:Pertussis Toxin; D011224:Prazosin; D004837:Epinephrine