Molecular cloning and structure of the human interleukin-5 gene.
We isolated the chromosomal gene for human interleukin-5 (IL-5) from human genomic libraries using a cloned human IL-5 cDNA as probe. Nucleotide sequence determination of the IL-5 gene and its flanking regions showed that the gene consisted of four exons and three introns. TATA-like and CAAT-like sequences reside 26 and 73 base pairs, respectively, upstream of the transcription initiation site identified by S1 mapping analysis. The 5'-flanking region of the IL-5 gene has sequences homologous with those of the corresponding regions of the genes for human granulocyte/macrophage colony-stimulating factor, murine IL-4, human interferon-gamma, and human IL-2. The intron-exon organization and location of the cysteine residue suggest that the IL-5 gene is phylogenetically related to the genes for the granulocyte/macrophage colony-stimulating factor, IL-4, and IL-2, although their amino acid sequences are not significantly conserved.
Tanabe
T
T
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
Konishi
M
M
Mizuta
T
T
Noma
T
T
Honjo
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003115:Colony-Stimulating Factors / Q000235:genetics; D003545:Cysteine / Q000032:analysis; D004720:Endonucleases / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015848:Interleukin-5; D007378:Interleukins / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D015719:Single-Strand Specific DNA and RNA Endonucleases
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D015848:Interleukin-5; D007378:Interleukins; D012333:RNA, Messenger; D007371:Interferon-gamma; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D003545:Cysteine