In situ protein-DNA interactions at a dioxin-responsive enhancer associated with the cytochrome P1-450 gene.
We used an in situ exonuclease III protection technique (C. Wu, Nature [London] 309:229, 1984) to analyze protein-DNA interactions at a dioxin-responsive enhancer. Our results imply that the 2,3,7,8-tetrachlorodibenzo-p-dioxin-receptor complex interacts with the dioxin-responsive enhancer to activate transcription of the cytochrome P1-450 gene.
Durrin
L K
LK
Department of Pharmacology, Stanford University School of Medicine, California 94305.
Whitlock
J P
JP
D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D003852:Deoxyribonucleoproteins / Q000378:metabolism*; D004147:Dioxins / Q000494:pharmacology*; D004742:Enhancer Elements, Genetic / Q000187:drug effects*; D005090:Exodeoxyribonucleases; D005796:Genes / Q000187:drug effects*; D005809:Genes, Regulator / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000378:metabolism; D014158:Transcription, Genetic
D002843:Chromatin; D003852:Deoxyribonucleoproteins; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug; D003577:Cytochrome P-450 Enzyme System; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III