title: Purification and properties of NADH dehydrogenase from a thermoacidophilic archaebacterium, Sulfolobus acidocaldarius.
abstract: An NADH dehydrogenase was purified to electrophoretical homogeneity from Sulfolobus acidocaldarius, a thermoacidophilic archaebacterium optimally growing at pH 2-3 and 75 degrees C. A 2,100-fold purification was achieved. The purified enzyme is an acidic protein with an isoelectric point of 5.6 and a molecular weight of 95,000, consisting of two 50,000-dalton subunits. The enzyme showed an absorption spectrum characteristic of flavoproteins, with maxima at 272, 372, and 448 nm. The enzyme is highly thermostable, is specific for NADH as an electron donor, and is capable of using 2,6-dichlorophenolindophenol, ferricyanide, benzoquinone, and naphthoquinone as electron acceptors. Though at a low rate, caldariellaquinone, a unique and sole benzothiophenequinone in the genus Sulfolobus, was also reduced by the enzyme, suggesting that the enzyme is a possible member of the respiratory chain of the thermoacidophilic archaebacterium.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, Kanagawa.
authors.forename: H
authors.initials: H
authors.lastname: Wakao
authors.forename: T
authors.initials: T
authors.lastname: Wakagi
authors.forename: T
authors.initials: T
authors.lastname: Oshima
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001105:Archaea / Q000201:enzymology*; D001419:Bacteria / Q000201:enzymology*; D002845:Chromatography; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003579:Cytochrome Reductases / Q000302:isolation & purification*; D017886:Durapatite; D006358:Hot Temperature; D006882:Hydroxyapatites; D007202:Indicators and Reagents; D007700:Kinetics; D008970:Molecular Weight; D009245:NADH Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D006882:Hydroxyapatites; D007202:Indicators and Reagents; D017886:Durapatite; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase