Histochemical demonstration of Cl(-)-ATPase in rat spinal motoneurons.
Using an enzyme-histochemical technique, rat spinal cords were stained for Cl(-)-ATPase, Na+,K+-ATPase and anion-insensitive Mg2+-ATPase. Cl(-)-ATPase activity was demonstrated in plasma membranes of spinal motoneurons, Na+,K+-ATPase activity and anion-insensitive Mg2+-ATPase activity were detected in neuronal plasma membranes and blood vessels, respectively.
Inagaki
C
C
Department of Biology, Kyoto Pharmaceutical University, Japan.
Oda
W
W
Kondo
K
K
Kusumi
M
M
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000032:analysis* / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D002712:Chlorides / Q000378:metabolism*; D004976:Ethacrynic Acid / Q000494:pharmacology; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008297:Male; D009046:Motor Neurons / Q000187:drug effects / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*
D002712:Chlorides; D010042:Ouabain; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D004976:Ethacrynic Acid