[Mapping the genome locus containing genes for mouse tumor necrosis factor by means of hybridization end labelling with the use of synthetic oligonucleotides].
We have developed a technique for restriction nuclease sites mapping in genomic DNA cloned into phage lambda vectors. Synthetic oligonucleotides homologous to the vector sequences and adjacent to the cloning site were used as hybridisation probes for indirect end labelling procedure. In addition, a number of oligonucleotides homologous to the sequences of tumour necrosis factor genes were used for mapping from the internal sites. As a result, a map of 35 kb genomic region on the chromosome 17 inside major histocompatibility complex and surrounding mouse tumour necrosis factor genes was constructed.
Nedospasov
S A
SA
Turetskaia
R L
RL
Mett
V A
VA
Shakhov
A N
AN
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D051379:Mice; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D009841:Oligonucleotides; D014409:Tumor Necrosis Factor-alpha
Kartirovanie genomnogo lokusa, soderzhashchego geny faktorov nekroza opukholeĭ myshi, metodom gibridizatsionnoĭ kontsevoĭ metki s ispol'zovaniem sinteticheskikh oligonukleotidov.