The detection of coxsackievirus RNA in cardiac tissue by in situ hybridization.
A cloned cDNA probe derived from coxsackie B4 virus-infected cell RNA was shown to hybridize to the RNA of a number of different enteroviruses including coxsackie A and B viruses, echoviruses and poliovirus. The probe was used to detect virus-specific RNA sequences in cardiac tissue obtained from patients diagnosed as having a coxsackievirus infection. Virus RNA was detected using the technique of in situ hybridization in 46% (6/13) cases, but none was found in normal, control, cardiac samples. Two distinct patterns of infection were observed. The significance of these differences and the possible uses of the technique are discussed.
Easton
A J
AJ
Department of Biological Sciences, University of Warwick, Coventry, U.K.
Eglin
R P
RP
D000208:Acute Disease; D003384:Coxsackievirus Infections / Q000382:microbiology*; D004247:DNA / Q000235:genetics; D029822:Enterovirus B, Human / Q000235:genetics / Q000302:isolation & purification*; D006321:Heart / Q000382:microbiology*; D006801:Humans; D009205:Myocarditis / Q000382:microbiology*; D009206:Myocardium / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*
D012367:RNA, Viral; D004247:DNA