Cloning and characterization of the gene, speC, for pyrogenic exotoxin type C from Streptococcus pyogenes.
The structural gene of streptococcal pyrogenic exotoxin type C (SPE C) was cloned from the chromosome of Streptococcus pyogenes strain T18P into Escherichia coli using pBR328 as the vector plasmid. Subcloning enabled the localization of the gene (speC) to a 1.7 kb fragment. Partially purified E. coli-derived SPE C and purified streptococcal-derived SPE C, were shown to have the same molecular weight (23,800) and biological activities. A DNA probe, prepared from cloned speC, cross-hybridized with the structural genes of SPE A and SPE B indicating relatedness at the nucleotide level. The speC-derived probe also hybridized to a fragment of CS112 bacteriophage DNA containing the phage attachment site.
Goshorn
S C
SC
University of Minnesota Medical School, Department of Microbiology, Minneapolis 55455.
Bohach
G A
GA
Schlievert
P M
PM
D001426:Bacterial Proteins; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005098:Exotoxins / Q000235:genetics* / Q000302:isolation & purification; D005796:Genes; D005798:Genes, Bacterial; D008565:Membrane Proteins; D010957:Plasmids; D011749:Pyrogens; D013297:Streptococcus pyogenes / Q000235:genetics*
D001426:Bacterial Proteins; D005098:Exotoxins; D008565:Membrane Proteins; D011749:Pyrogens; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin; D004262:DNA Restriction Enzymes