GAL4 activates gene expression in mammalian cells.
GAL4, a protein that activates transcription in yeast, is shown to activate the mouse mammary tumor virus promoter in mammalian cells. Activation depends upon a GAL4-binding sequence inserted upstream of the gene. Deletion mutants of GAL4 bearing one or both of the "activating regions" required for activation in yeast also activate transcription in mammalian cells. A derivative of GAL4 that binds to DNA but cannot activate transcription in yeast also fails to activate transcription in mammalian cells. We also show that GAL4 and the glucocorticoid receptor activate the mouse mammary tumor virus promoter synergistically.
Kakidani
H
H
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Ptashne
M
M
D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D003907:Dexamethasone / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics*; D005938:Glucocorticoids / Q000494:pharmacology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011965:Receptors, Glucocorticoid / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003907:Dexamethasone; D004247:DNA; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase