Microassay for measurement of binding of radiolabelled ligands to cell surface molecules.
An improved technique for measuring the binding of radiolabelled ligands to cell surface molecules has been developed by modification of a procedure using centrifugation through a water-immiscible oil to separate free and cell-bound ligand. It maximises the percentage of ligand bound since cell-bound and free ligand can be separated easily and reproducibly even when very small reaction volumes are used. This permits low levels of ligand radiolabelling and relatively low numbers of cells to be used.
Woof
J M
JM
Department of Biochemistry, University of Sheffield, U.K.
Burton
D R
DR
D002460:Cell Line; D002498:Centrifugation; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D066298:In Vitro Techniques; D008832:Microchemistry; D009821:Oils; D011869:Radioligand Assay / Q000379:methods*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011961:Receptors, Fc / Q000378:metabolism; D017452:Receptors, IgG
D007075:Immunoglobulin M; D009821:Oils; D011956:Receptors, Cell Surface; D011961:Receptors, Fc; D017452:Receptors, IgG