title: The ontogeny of brain neurotensin receptors studied by autoradiography.
abstract: Using in vitro receptor autoradiographic techniques we have analysed the pre- and postnatal development of neurotensin receptors in the rat brain. Receptors were labeled with [3H] or [125I]neurotensin in mounted tissue sections from animals of ages gestational day 14 until the postnatal day 21 as well as young adult animals. Very low densities of neurotensin receptors were visualized on gestational days 14 and 15. Between gestational days 16 and 18 a marked increase in the density of neurotensin receptors was seen in the developing neocortex. Densities in other brain areas, particularly the midbrain and brainstem were much lower than cortical densities. The density of neurotensin receptors in the cortex increased through the last part of the gestation and early postnatal life until it peaked at the end of the first postnatal week. After that, neurotensin receptor binding decreased dramatically reaching lower densities seen in the adult animal at the end of the third postnatal week. Development of neurotensin receptors in other brain areas followed very different time patterns. Neurotensin receptors in the midbrain were seen first at gestational day 18 and increased slowly with development to reach adult levels at about the second week of postnatal life. Neurotensin receptors in the hippocampal formation demonstrated postnatal development; they were detected at postnatal day 5 and showed a developmental peak around the second week. These patterns were seen with both 3H- and 125I-labeled neurotensin, thus excluding possible differential quenching artifacts. These clear differential regional ontogenetic patterns for neurotensin receptors are the main findings of these experiments. The very high densities present in the cortex even in fetal stages suggest that neurotensin could play a role in the development of the brain.
authors.affiliation: Preclinical Research, SANDOZ Ltd., Basle, Switzerland.
authors.forename: J M
authors.initials: JM
authors.lastname: Palacios
authors.forename: A
authors.initials: A
authors.lastname: Pazos
authors.forename: M M
authors.initials: MM
authors.lastname: Dietl
authors.forename: M
authors.initials: M
authors.lastname: Schlumpf
authors.forename: W
authors.initials: W
authors.lastname: Lichtensteiger
mesh_terms: D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000254:growth & development*; D005260:Female; D009496:Neurotensin / Q000378:metabolism; D051381:Rats; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter / Q000032:analysis*
chemical_list: D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter; D009496:Neurotensin