title: The nucleotide sequence and in situ localization of a gene for a dimeric haemoglobin from the midge Chironomus thummi piger.
abstract: A cluster containing at least four globin genes was isolated by screening an lambda EMBL3 genomic DNA library of the midge Chironomus thummi piger (Ctp) with a heterologous haemoglobin (Hb) gene IV (HbIV) probe from Chironomus thummi thummi (Ctt). This globin gene cluster was localized by in situ hybridization to chromosome II. One globin gene together with its 5'- and 3'-flanking regions has been sequenced. It can be deduced from the sequence that it is a new member of the dimeric HbVIIB family. The Ctp HbVIIB-5 gene displays 91.8% nucleotide sequence homology to a HbVIIB cDNA sequence, reported previously. There is no evidence for intron/exon structure in the Ctp HbVIIB-5 gene.
authors.affiliation: Ruhr-Universität Bochum, Institut für Genetik, F.R.G.
authors.forename: T
authors.initials: T
authors.lastname: Hankeln
authors.forename: P
authors.initials: P
authors.lastname: Rozynek
authors.forename: E R
authors.initials: ER
authors.lastname: Schmidt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004175:Diptera / Q000235:genetics*; D005796:Genes; D006454:Hemoglobins / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D004262:DNA Restriction Enzymes