Responses of neurons in the rat nucleus submedius to noxious and innocuous mechanical cutaneous stimulation.
Extracellular recordings were used to characterize responses to cutaneous mechanical stimulation of 78 neurons in the rat nucleus submedius (SM). Thirty-nine of these units were activated by some type of cutaneous mechanical stimulation. Eighteen cells were activated exclusively by noxious stimuli. In 13 of these cells, responses were of swift onset and relatively rapid termination following stimulus application. In contrast, in three neurons responses were delayed both in onset and termination, and in two the response was immediate, but the markedly increased evoked activity outlasted stimulus application by 13 min. Receptive fields (RFs) of these nociceptive neurons were generally large, although none were bilateral. Four SM neurons were activated by innocuous stimuli, but their maximal response was obtained only after noxious stimulation. Responses of all of these neurons were of immediate onset and recovery, and their RFs were large (two were bilateral). Twelve SM neurons were activated maximally by innocuous stimuli. Responses of seven of these cells were immediate in onset and termination, while that of three were delayed in both onset and termination. Two of the 12 innocuous-only neurons quickly became unresponsive to repeated stimulus applications, and could be reactivated only after a rest period during which no stimuli were applied. RFs of these units were also generally large, and in three cases were bilateral. Five SM neurons responded by decreasing, or completely ceasing, their firing subsequent to noxious-only (n = 2), or innocuous-only (n = 3) stimulation. Four of these units had large RFs (two were bilateral). The remaining 39 SM neurons could not be activated by any type of mechanical cutaneous stimulation we tried. Electrical stimulation of the ventrolateral orbital cortex (VLO) was employed to examine frontal cortical projections of 21 SM neurons. Ten of these units were activated, although all of them synaptically rather than antidromically, and two were inhibited. There was no clear-cut relationship between neuronal location, physiological type, RF site, or VLO stimulation effects among the 39 SM neurons. These results provide further support for the involvement of SM neurons in nociceptive information signaling, and suggest additionally that the role of the nucleus is not limited to nociception but encompasses a wider range of cutaneous sensations.
Miletic
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Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.
Coffield
J A
JA
D000200:Action Potentials; D000818:Animals; D004558:Electric Stimulation; D005625:Frontal Lobe / Q000502:physiology; D008442:Maxillary Nerve / Q000502:physiology; D008465:Mechanoreceptors / Q000502:physiology*; D010146:Pain / Q000503:physiopathology*; D010812:Physical Stimulation; D051381:Rats; D011919:Rats, Inbred Strains; D011930:Reaction Time / Q000502:physiology; D012867:Skin / Q000294:innervation*; D013787:Thalamic Nuclei / Q000502:physiology* / Q000503:physiopathology