title: [DNA amplification using the polymerase chain reaction method applied to the detection of HIV-1 in seropositive subjects and in seronegative subjects at risk].
abstract: Polymerase Chain Reaction (PCR) allows the amplification of specific segments of Human Immunodeficiency Virus (HIV) genome. This technique is of interest for direct detection of HIV-1 DNA sequences in peripheral blood mononuclear cells. In our study, 54 out of 55 HIV-1 seropositive subjects were found positive with PCR assay. No detection of HIV DNA was observed in 36 seronegative at risk subjects (16 homosexuals and 20 polytransfused haemophiliacs), in 20 subjects with isolated and persistent anti-core antibodies, in 20 thalassemic polytransfused children and in 74 HIV seronegative blood donors (negative controls). These results are consistent with those of classical HIV serology and indicate that latent HIV-1 infection in seronegative at risk subjects is not a frequent event, if it exists.
authors.affiliation: Institut National de Transfusion Sanguine, Université Pierre et Marie Curie (UER St-Antoine), Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Mariotti
authors.forename: J J
authors.initials: JJ
authors.lastname: Lefrère
authors.forename: B
authors.initials: B
authors.lastname: Noël
authors.forename: F
authors.initials: F
authors.lastname: Ferrer-le-Coeur
authors.forename: D
authors.initials: D
authors.lastname: Vittecoq
authors.forename: R
authors.initials: R
authors.lastname: Girot
authors.forename: A M
authors.initials: AM
authors.lastname: Courouce
authors.forename: C
authors.initials: C
authors.lastname: Salmon
authors.forename: P
authors.initials: P
authors.lastname: Rouger
mesh_terms: D000328:Adult; D001483:Base Sequence; D001803:Blood Transfusion; D002648:Child; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D005784:Gene Amplification; D006678:HIV / Q000302:isolation & purification*; D006679:HIV Seropositivity / Q000097:blood / Q000382:microbiology*; D006467:Hemophilia A / Q000097:blood; D006716:Homosexuality; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D013789:Thalassemia / Q000097:blood
chemical_list: D004279:DNA, Viral
vernacular_title: Amplification de l'ADN par la méthode de "Polymerase Chain Reaction" (PCR) appliquée à la détection de séquences VIH-1 chez des sujets séropositifs et des sujets séronégatifs à risque.