title: Demonstration of the presence of G-proteins in hepatic microsomal fraction.
abstract: The presence of G-proteins in isolated hepatic microsomal vesicles is demonstrated. The G-proteins were identified by their capacity to be ADP-ribosylated by cholera and pertussis toxins. Cholera toxin identified 42 and 45 kDa proteins, corresponding to alpha s-1 and alpha s-2, respectively. Pertussis toxin identified a 40 kDa protein corresponding to alpha i. The microsomal G-proteins are identical to the corresponding G proteins of the plasma membrane, but are present in different proportions; the microsomes have considerably less alpha s proteins than the plasma membrane.
authors.affiliation: Department of Cell Biology, Baylor College of Medicine, Houston, Texas.
authors.forename: J
authors.initials: J
authors.lastname: Codina
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: N
authors.initials: N
authors.lastname: Kraus-Friedmann
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002772:Cholera Toxin / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D008970:Molecular Weight; D037342:Pertussis Toxin; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000378:metabolism
chemical_list: D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D002772:Cholera Toxin; D037342:Pertussis Toxin; D005952:Glucose-6-Phosphatase; D019204:GTP-Binding Proteins; D000254:Sodium-Potassium-Exchanging ATPase