Ki-ras-2 in acute lymphoblastic leukemia cells with chromosome change at 12p12.
Cytogenetic and molecular investigations were performed on a pre-B-lymphoblastic acute leukemic cell line (NALM-6). The NALM-6 cells contained a del(5)(q32) and an ins(12)(p12;?), chromosomal material of unknown origin being inserted between subbands 12p12.1 and 12p12.2. Chromosomal in situ hybridization using a 3.0-kb c-Ki-ras-2 probe showed a significant accumulation of grains on the proximal portion of the inserted chromosomal material (12p12.1), as well as on the normal chromosome #12 with a peak at 12p11p12. The signal intensity obtained after hybridization of the c-Ki-ras-2 specific probe to the NALM-6 cells DNA is comparable with the intensity of the signal after hybridization of the same probe with the control cell line (MC/B) DNA. The findings indicate that the c-Ki-ras-2 gene is neither amplified nor transposed in the NALM-6 cells.
Ohyashiki
K
K
Department of Genetics and Endocrinology, Roswell Park Memorial Institute, Buffalo, NY.
Ohyashiki
J H
JH
Kinniburgh
A J
AJ
Toyama
K
K
Ito
M
M
Minowada
J
J
Sandberg
A A
AA
D002871:Chromosome Banding; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D011905:Genes, ras; D006801:Humans; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000235:genetics*; D011519:Proto-Oncogenes; D014407:Tumor Cells, Cultured
D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII