Chicken embryo propagation of type I avian adenoviruses.
Forty-two clone-purified, cell-culture-propagated type I avian adenoviruses (AAV) representing 11 serotypes and two intermediate strains were evaluated for virus replication (evidenced by embryo death and lesions) resulting from the inoculation of specific-pathogen-free chicken embryos via the chorioallantoic sac or yolk sac. Commonly observed embryonic changes were death, stunting and curling, hepatitis, splenomegaly, congestion and hemorrhage of body parts, and urate formation in the kidneys. Basophilic or eosinophilic intranuclear inclusion bodies characteristic of fowl adenoviruses were observed in hepatocytes. The magnitude and relative uniformity of intra- and interserotypic embryo mortality, gross lesions, and virus titers was greater in embryos inoculated via the yolk sac. This work identifies the yolk sac as a practical and sensitive chicken embryo inoculation route for poultry diagnosticians to employ. It is suggested that the yolk sac may be a reliable alternative to cell culture for the successful isolation of all type I avian adenoviruses.
Cowen
B S
BS
Department of Veterinary Science, College of Agriculture, Pennsylvania State University, University Park 16802.
D000256:Adenoviridae / Q000254:growth & development*; D000482:Allantois / Q000382:microbiology; D000818:Animals; D000259:Aviadenovirus / Q000254:growth & development*; D002642:Chick Embryo / Q000382:microbiology*; D002823:Chorion / Q000382:microbiology; D008099:Liver / Q000473:pathology; D014776:Virus Cultivation / Q000662:veterinary*; D015017:Yolk Sac / Q000382:microbiology