Benzoate-dependent induction from the OP2 operator-promoter region of the TOL plasmid pWWO in the absence of known plasmid regulatory genes.
Expression of the lower catabolic pathway of the TOL plasmid pWWO requires an aromatic acid inducer and the product of the xylS regulatory gene. Pseudomonas putida cells transformed with a plasmid containing the operator-promoter region of the lower pathway (OP2 [or Pm]), upstream from the catechol 2,3-dioxygenase structural gene, showed enzyme induction in the absence of known TOL plasmid regulatory genes. Induction was not seen in transformed Escherichia coli cells or in a P. putida mutant lacking chromosomally encoded benzoate catabolic functions.
Cuskey
S M
SM
Environmental Research Laboratory, United States Environmental Protection Agency, Gulf Breeze, Florida.
Sprenkle
A B
AB
D001565:Benzoates / Q000494:pharmacology; D050557:Catechol 2,3-Dioxygenase; D004262:DNA Restriction Enzymes; D049308:Dioxygenases; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009875:Operator Regions, Genetic; D010105:Oxygenases / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity; D014169:Transformation, Bacterial
D001565:Benzoates; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D004262:DNA Restriction Enzymes