Intracellular transport of VSV G protein occurs in cells lacking a nuclear envelope.
The intracellular transport of the G protein of a temperature sensitive vesicular stomatitis virus (VSV) mutant (ts 045) was examined in nucleated chinese hamster ovary (CHO) cells and in CHO cells subjected to enucleation with cytochalasin B. Although protein synthesis was virtually unaffected, enucleated cells synthesized only 30-45% of the amount of G protein assembled in control cells. As measured by acquisition of endoglycosidase H resistance, the rate of transport of the G protein from the RER to the medial golgi was found to be similar for nucleated and enucleated cells (t1/2 = 15 min). These data suggest that elements of the RER may directly transfer their contents to the Golgi, without the obligatory involvement of an intact NE.
Hanover
J A
JA
NIDDK, Bethesda, MD 20892.
D000818:Animals; D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D007700:Kinetics; D008562:Membrane Glycoproteins; D008970:Molecular Weight; D009154:Mutation; D009685:Nuclear Envelope / Q000187:drug effects / Q000502:physiology*; D013696:Temperature; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins / Q000096:biosynthesis / Q000378:metabolism*
C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D003571:Cytochalasin B