Association of SV40 DNA sequences with nuclear matrix in SV40 transformed hamster fibroblasts.
Nuclear matrices were isolated by the high-salt, non-ionic detergent method from SV40-transformed hamster fibroblasts (TSV5 cell line), and from hamster tumours derived from these cells. DNA isolated from matrices and total nuclei was hybridized with nick-translated SV40 DNA. The enrichment of matrix DNA with SV40 DNA sequences was observed in all five experiments with matrix DNA of TSV5 cells but only in five out of nine matrix DNA isolated from tumour cells.
Rzeszowska-Wolny
J
J
Department of Tumour Biology, Institute of Oncology, Gliwice, Poland.
Lanuszewska
J
J
Chorazy
M
M
D000818:Animals; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000032:analysis; D006224:Cricetinae; D004279:DNA, Viral / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D013539:Simian virus 40 / Q000235:genetics*; D014412:Tumor Virus Infections / Q000235:genetics*
D004279:DNA, Viral