Production of monoclonal antibodies against equine influenza: application to a comparative study of various strains of the virus.
Monoclonal antibodies (Mo Abs) were prepared against influenza/A/equine/Prague/1/56 (H7N7) and influenza/A/equine/Miami/1/63 (H3N8) reference strains of equine influenza virus. These monoclonals were tested against the 2 reference strains, 8 field strains of equine influenza virus, 3 human influenza viruses possessing the H3 hemagglutinin, and one virus of human origin possessing the H1 hemagglutinin. Two antibodies were obtained in one fusion against the Prague/1/56 strain and reacted only with this strain. Four anti/A/equine/Miami/1/63 Mo Abs were obtained in one fusion. They differentiated 8 strains of equine origin from all strains of human origin and from one strain of equine origin (Joinville/1/78) isolated in 1978. The specificity of this difference was confirmed by cross-seroneutralization between A/equine/Miami/1/63 strain and A/equine/Joinville/1/78 strain.
Crucière
C
C
Ministère de l'Agriculture, Laboratoire central de recherches vétérinaires, Maisons-Alfort, France.
Guillemin
M C
MC
Roseto
A
A
Wirbel
A
A
Plateau
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D006736:Horses; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral