[Detection of cytomegalovirus (CMV) DNA sequence in biopsy samples of cervical cancer].
Biopsy samples of the cervical cancer and cervicitis were analysed by HCMV-DNA hybridization method. The results indicated that HCMV-DNA was detected in 21 (36%) of 59 cervical cancer and 6 (21%) of 28 cervicitis specimens, using 32P-DNA probe containing transformation gene (14% of total genome) to hybridize. None of 25 cervical cancer and 19 cervicitis samples showed positive HCMV-DNA, using 32P-DNA probe without transformation gene (76% of total genome). The preliminary results show that the sequence of CMV transformation gene is present in about one third of cervical cancer and one fifth of cervicitis biopsy samples tested. This detection rate is lower than that of Taiwan Province and Africa, and slightly higher than that of the USA and Finland. The relation between the HCMV and cervical cancer is being further studied.
Wang
S F
SF
Fourth Hospital, Hebei Medical College, Shijiazhuang.
Zhao
Y
Y
Fu
Q L
QL
Yin
M B
MB
Liu
Y Y
YY
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000032:analysis* / Q000382:microbiology; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D002583:Uterine Cervical Neoplasms / Q000032:analysis* / Q000382:microbiology; D002575:Uterine Cervicitis / Q000382:microbiology
D004279:DNA, Viral