Changes in the three-dimensional organization of LoVo cells associated with resistance to doxorubicin.
LoVo cells and a derived subline resistant to doxorubicin were compared in the spheroids system. The resistant line, unlike the parent one, was unable to grow as spheroids, but formed irregular loose aggregates. Moreover treatment of the resistant cells with membrane-active agents able to reverse pleiotropic drug resistance had no effect on the capability of these cells to grow as spheroids. The results indicate that the inability of resistant cells to form spheroids is not related to the resistance mechanism.
Soranzo
C
C
Division of Experimental Oncology B, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Ingrosso
A
A
Buffa
M
M
Della Torre
G
G
Gambetta
R A
RA
Zunino
F
F
D000230:Adenocarcinoma / Q000473:pathology; D002449:Cell Aggregation; D002455:Cell Division; D003110:Colonic Neoplasms / Q000473:pathology; D003524:Cyclosporins / Q000494:pharmacology; D004317:Doxorubicin; D004351:Drug Resistance; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D009940:Organoids; D014268:Trifluoperazine / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology*; D014700:Verapamil / Q000494:pharmacology
D003524:Cyclosporins; D008565:Membrane Proteins; D014268:Trifluoperazine; D004317:Doxorubicin; D014700:Verapamil