Secondary structure in properdin of the complement cascade and related proteins: a study by Fourier transform infrared spectroscopy.
Six structural repeat motifs of 58 amino acids are found in the sequence of both mouse and human properdins. Twelve more examples of the motif are available from the sequences of thrombospondin, the terminal complement components, and the thrombospondin-related anonymous protein. The averaged Robson and Chou-Fasman secondary structure predictions show that there are 57-66% turn and 19-38% beta-sheet structures in the typical repeat motif. The high amount of turn structure is consistent with Gly, Pro, Cys, and Ser being the four most abundant amino acid residues in properdin. Comparisons with sequences found in the circumsporozoite protein from several species of malaria parasites show that their sequences and secondary structures strongly coincide only in a 18-residue segment. Further secondary structure analysis utilized Fourier transform infrared spectroscopy of human properdin in 2H2O buffers. These show a broad amide I band that, after second-derivative and deconvolution calculations, is shown to be composed of several components. Two at 1633 and 1683 cm-1 are strong evidence for beta-sheet structure, although overlap from beta-turns can also contribute. The presence of beta-turn structure is indicated by absorptions at 1662-1675 and 1645 cm-1. The properdin structure contains substantial quantities of beta-sheet and beta-turn structures, which is consistent with the secondary structure predictions and amino acid compositions. The length of the repeat motif is estimated as 3.3-4.3 nm, and an estimated 14-22% of nonexchanged amide protons reside in properdin. This is suggestive of a high degree of solvent accessibility in the structure.
Perkins
S J
SJ
Department of Biochemistry and Chemistry, Royal Free Hospital School of Medicine, London, U.K.
Nealis
A S
AS
Haris
P I
PI
Chapman
D
D
Goundis
D
D
Reid
K B
KB
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan; D005583:Fourier Analysis; D006801:Humans; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D010961:Plasmodium; D011414:Properdin; D011487:Protein Conformation; D015800:Protozoan Proteins; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D019699:Thrombospondins
D000953:Antigens, Protozoan; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D019699:Thrombospondins; C041595:circumsporozoite protein, Protozoan; D011414:Properdin