Assay of nucleoside-diphosphate kinase activity by the coupled nucleotidyltransferase.
A method coupled with Escherichia coli RNA polymerase is described for assaying nucleoside-diphosphate kinase activity. The principle of the procedure is indirect but allows processing of a large number of samples by employing filter-paper disk techniques.
Sato
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Facilities for Comparative Medicine & Animal Experimentation, Niigata University, School of Medicine, Japan.
D000244:Adenosine Diphosphate / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D009701:Nucleoside-Diphosphate Kinase / Q000032:analysis*; D009713:Nucleotidyltransferases; D010770:Phosphotransferases / Q000032:analysis*
D000244:Adenosine Diphosphate; D010770:Phosphotransferases; D009701:Nucleoside-Diphosphate Kinase; D009713:Nucleotidyltransferases; D012321:DNA-Directed RNA Polymerases