title: In vitro degradation of amyloid material by four proteases in tissue of a patient with familial amyloidotic polyneuropathy.
abstract: The effects of 4 proteolytic enzymes, alpha-chymotrypsin, bromeline, collagenase, and lysozyme on amyloid tissue sections from a patient with familial amyloidotic polyneuropathy (FAP) were evaluated. Degradation of amyloid fibrils was significant with alpha-chymotrypsin, moderate with bromeline and collagenase, and slight with lysozyme. All of these proteases except collagenase are used as oral mucolytics in humans. The possibility of their clinical usefulness in the treatment or prevention of the development of FAP is discussed.
authors.affiliation: Department of Medicine (Neurology), Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Adachi
authors.forename: C S
authors.initials: CS
authors.lastname: Koh
authors.forename: N
authors.initials: N
authors.lastname: Tsukada
authors.forename: S
authors.initials: S
authors.lastname: Shoji
authors.forename: N
authors.initials: N
authors.lastname: Yanagisawa
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D000686:Amyloidosis / Q000201:enzymology* / Q000235:genetics; D001963:Bromelains / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D003012:Microbial Collagenase / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D010523:Peripheral Nervous System Diseases / Q000201:enzymology* / Q000235:genetics
chemical_list: D000682:Amyloid; D001963:Bromelains; D009113:Muramidase; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D003012:Microbial Collagenase