Effect of diterpene esters on actin cytoskeleton of SV40-transformed keratinocytes is not reproduced by diacylglycerols.
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a disorganization of the actin cytoskeleton and its redistribution at the periphery of the cells in SV40-transformed human keratinocytes. This phenomenon is induced by other diterpene esters, such as 4-O-methyl TPA, 12-O-ethacrynylphorbol-13-acetate (EPA), 12-O-retinoylphorbol-13-acetate (RPA) and mezerein, which exert either convertogenic or promoting effects in skin tumor development in vivo. Two diacylglycerols: oleyl-acetyl glycerol (OAG) and dioctanoyl-glycerol (DOG) do not induce the disorganization of actin. Thus, the effects of these compounds, as they are found in SV40-transformed human keratinocytes, do not exhibit clear-cut correlations to either their protein kinase C activating abilities, or their effects on different stages of multistage carcinogenesis in mouse skin in vivo.
Delescluse
C
C
Cell Biology Department, Centre International de Recherches Dermatologiques, Valbonne, France.
Bernard
B A
BA
Fürstenberger
G
G
Sorg
B
B
Marks
F
F
Bailly
J
J
Darmon
M
M
D000199:Actins; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000648:ultrastructure; D002472:Cell Transformation, Viral / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D004075:Diglycerides / Q000633:toxicity*; D004224:Diterpenes / Q000633:toxicity*; D004952:Esters / Q000633:toxicity; D005989:Glycerides / Q000633:toxicity*; D006801:Humans; D013539:Simian virus 40; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D000199:Actins; D004075:Diglycerides; D004224:Diterpenes; D004952:Esters; D005989:Glycerides; D013755:Tetradecanoylphorbol Acetate