The effectiveness of postischemic oxypurinol administration upon myocardial function in the isolated rat heart.
The Langendorff isolated rat heart preparation was used to determine the effect of oxypurinol, a xanthine oxidase inhibitor, on myocardial function when administered during reperfusion after 30 min of warm ischemia. Twenty rats were randomly sorted into 2 groups of 10, and an isolated heart preparation made from each rat. The isolated hearts were perfused for 15 min with a modified Krebs-Henseleit solution to permit stabilization of the preparation. Each heart was then subjected to 30 min of total ischemia at 37 degrees C followed by 40 min of reperfusion with either saline-treated perfusate or oxypurinol-treated perfusate (1.3 mM). The maximum power produced and the preload required to produce maximum power were both determined prior to ischemia and every 10 min after ischemia during 40 min of reperfusion. The saline-treated group, but not the oxypurinol-treated group, showed significantly less maximum power output at all testing times during reperfusion compared to the preischemic value (P less than 0.05). There was a significantly greater maximum power output (P less than 0.02) in the oxypurinol-treated group compared to the saline-treated group after 20, 30, and 40 min of reperfusion. There were no differences within either group, or between groups, for the preload required to produce maximum power at any of the testing times. Ultrastructural examination of myocardium after reperfusion showed severe mitochondrial and myofibrillar disruption in the saline-treated group but not in the oxypurinol-treated group. We conclude that oxypurinol administered following 30 min of total ischemia at the onset of reperfusion, can preserve myocardial function during the early reperfusion period in the isolated rat heart.
Badylak
S F
SF
Hillenbrand Biomedical Engineering Center, Purdue University, W. Lafayette, IN 47907.
Voorhees
W D
WD
Babbs
C F
CF
Simmons
A
A
D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects*; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D007511:Ischemia; D008297:Male; D009206:Myocardium / Q000648:ultrastructure; D010100:Oxygen / Q000502:physiology; D010117:Oxypurinol / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012151:Resuscitation
D011743:Pyrimidines; D010117:Oxypurinol; D010100:Oxygen