title: Two distinct receptors for ATP can be distinguished in Swiss 3T6 mouse fibroblasts by their desensitization.
abstract: The stimulation of calcium efflux from Swiss 3T6 mouse fibroblasts by extracellular ATP was studied. It was found that the cells could be desensitized to ATP by a previous exposure to the nucleotide, lending support to the theory that this is a receptor mediated process. Another ATP-receptor mediated process in Swiss 3T6 cells, that is also subject to desensitization, causes the permeabilization of the plasma membrane to nucleotides and other normally impermeant compounds [Gonzalez et al., J. Cell. Physiol. 139:109 (1989)]. Here we demonstrate that selective desensitization of the ATP-dependent calcium mobilization pathway can be achieved without affecting ATP-induced permeabilization. Data are presented in support of the existence of multiple ATP-receptors (purinoceptors) in Swiss 3T6 cells.
authors.affiliation: Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.
authors.forename: F A
authors.initials: FA
authors.lastname: Gonzalez
authors.forename: E
authors.initials: E
authors.lastname: Bonapace
authors.forename: I
authors.initials: I
authors.lastname: Belzer
authors.forename: I
authors.initials: I
authors.lastname: Friedberg
authors.forename: L A
authors.initials: LA
authors.lastname: Heppel
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002122:Calcium Chloride / Q000378:metabolism; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D011983:Receptors, Purinergic / Q000187:drug effects / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000494:pharmacology
chemical_list: D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate; D002122:Calcium Chloride; D014544:Uridine Triphosphate