title: Interaction of origin binding protein with an origin of replication of herpes simplex virus 1.
abstract: Herpes simplex virus 1 encodes a protein that binds specifically to a viral DNA replication origin (oriS). This origin-binding protein has been purified to homogeneity by means of sequence-specific DNA affinity chromatography. The purified origin-binding protein, which has a molecular weight of 83,000, interacts with both parts of the oriS dyad; however, the affinity for the two sites differs by an order of magnitude. The sequence TTCGCA occurs as a nearly perfect direct repeat within the two sites and may constitute the recognition sequence for the origin-binding protein.
authors.affiliation: Department of Medical Biochemistry, University of Gothenburg, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Elias
authors.forename: I R
authors.initials: IR
authors.lastname: Lehman
mesh_terms: D001483:Base Sequence; D002846:Chromatography, Affinity; D004279:DNA, Viral / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012091:Repetitive Sequences, Nucleic Acid; D018139:Simplexvirus / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; C055789:origin-binding proteins, viral