title: Autoradiographic demonstration of gastrin-releasing peptide-binding sites in the rat gastric mucosa.
abstract: The location of [125I]iodotyrosyl gastrin-releasing peptide-binding sites in the rat fundic mucosa was studied. Peptide specificity was demonstrated by competitive binding studies using the addition of a large amount of cold gastrin-releasing peptide or substance P. Autoradiography of the stomach tissue was carried out by freeze-drying, embedding in Epon, wet-sectioning with ethylene glycol, and dry-mounting the emulsion film by the wire-loop method to prevent loss of the labeled substance. Specific binding sites of gastrin-releasing peptide were found on D cells, surface mucus cells, and parietal cells, whereas few binding sites were seen on the chief or mucus neck cells.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Oda
authors.forename: K
authors.initials: K
authors.lastname: Kaneko
authors.forename: Y
authors.initials: Y
authors.lastname: Akaiwa
authors.forename: N
authors.initials: N
authors.lastname: Tsukada
authors.forename: H
authors.initials: H
authors.lastname: Komatsu
authors.forename: M
authors.initials: M
authors.lastname: Tsuchiya
mesh_terms: D000818:Animals; D001345:Autoradiography; D001839:Bombesin / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D019886:Gastrin-Releasing Peptide; D005755:Gastrins / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D010295:Parietal Cells, Gastric / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter / Q000378:metabolism*
chemical_list: D005755:Gastrins; D007457:Iodine Radioisotopes; D010455:Peptides; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter; D019886:Gastrin-Releasing Peptide; D001839:Bombesin