title: In vivo regulation of glycolytic and gluconeogenic enzyme gene expression in newborn rat liver.
abstract: Glucagon and its second messenger, cAMP, are known to rapidly block expression of the L-type pyruvate kinase gene and to stimulate expression of phosphoenolpyruvate (PEP) carboxykinase gene in the liver in vivo. The respective roles, however, of hyperglucagonemia, insulinopenia, and carbohydrate deprivation in the inhibition of L-type pyruvate kinase gene expression during fasting are poorly understood. In addition, the long-term effects of physiological hyperglucagonemia on expression of the two genes are not known. In this study, we investigate the effects of long-term physiological hyperglucagonemia and insulinopenia induced by suckling (which provides a high-fat, low-carbohydrate diet) on expression of the two genes in the liver of normal newborn rats. We show that transcription of the L-type pyruvate kinase gene is inhibited at birth and remains low during the whole suckling period, whereas transcription of the PEP carboxykinase gene is maximal in the neonate, and then decreases despite very high levels of plasma glucagon during suckling. In contrast to the adult, however, in which L-type pyruvate kinase gene expression in the liver is blocked by cAMP and stimulated by carbohydrates, the regulation of L-type pyruvate kinase gene expression in the newborn undergoes a developmental maturation: the inhibitory effect of glucagon is never complete in developing rat liver and the stimulatory effect of glucose could not be detected during suckling, due to either hyperglucagonemia, immaturity of the gene regulatory system, or both.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U129, Unité de Génétique et Pathologie Moléculaires, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Lyonnet
authors.forename: C
authors.initials: C
authors.lastname: Coupé
authors.forename: J
authors.initials: J
authors.lastname: Girard
authors.forename: A
authors.initials: A
authors.lastname: Kahn
authors.forename: A
authors.initials: A
authors.lastname: Munnich
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D000242:Cyclic AMP / Q000494:pharmacology; D005786:Gene Expression Regulation; D005934:Glucagon / Q000097:blood / Q000502:physiology*; D005943:Gluconeogenesis; D005947:Glucose / Q000502:physiology*; D006019:Glycolysis; D007328:Insulin / Q000097:blood; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*; D014176:Protein Biosynthesis; D011770:Pyruvate Kinase / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
chemical_list: D007328:Insulin; D012333:RNA, Messenger; D005934:Glucagon; D000242:Cyclic AMP; D011770:Pyruvate Kinase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D005947:Glucose