title: GTP-binding protein associated with amino acid binding proteins from olfactory epithelium of skate, Dasyatis pastinaca.
abstract: Amino acid binding protein (98 kDa) from olfactory epithelium of skate, Dasyatis pastinaca, form a stable complex with the 56 kDa protein. This complex dissociates in the presence of 10 microM GTP gamma S and 2 mM MgCl2. The 56 kDa protein has a steady-state GTPase activity (15 nmol/min per mg). Binding of amino acids to the 98 kDa protein specifically stimulates GTPase activity of the 56 kDa protein; half-maximal stimulation of GTPase activity is observed at 0.1 microM amino acid.
authors.affiliation: Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region.
authors.forename: V I
authors.initials: VI
authors.lastname: Novoselov
authors.forename: L D
authors.initials: LD
authors.lastname: Krapivinskaya
authors.forename: G B
authors.initials: GB
authors.lastname: Krapivinsky
authors.forename: E E
authors.initials: EE
authors.lastname: Fesenko
mesh_terms: D000409:Alanine / Q000494:pharmacology; D000818:Animals; D004848:Epithelium / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D009831:Olfactory Mucosa / Q000378:metabolism*; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D012860:Skates, Fish
chemical_list: D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D000409:Alanine