title: Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus.
abstract: A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.
authors.affiliation: Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Chisaka
authors.forename: K
authors.initials: K
authors.lastname: Araki
authors.forename: T
authors.initials: T
authors.lastname: Ochiya
authors.forename: T
authors.initials: T
authors.lastname: Tsurimoto
authors.forename: W
authors.initials: W
authors.lastname: Hiranyawasitte-Attatippaholkun
authors.forename: N
authors.initials: N
authors.lastname: Yanaihara
authors.forename: K
authors.initials: K
authors.lastname: Matsubara
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000382:microbiology; D002460:Cell Line; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms; D010957:Plasmids; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: D014764:Viral Proteins