Biological and biochemical comparison of bovid herpesvirus-4 strains.
Bovid herpesvirus-4 (BHV-4) isolates V.Test and LVR140, isolated from genital disease, respectively, in bull and in cow, and the reference strains Movar 33/63 and DN599 were compared by several methods: cross-serological relationship studied by indirect immunofluorescence; kinetics of intracellular and extracellular viral production; comparison of the mean plaque size; restriction analysis of viral DNA with restriction enzymes EcoRI, BamHI and HindIII. BHV-4 strains were serologically identical and the kinetics of viral production were very similar. Comparison of the mean plaque size allowed classification into 3 classes (Class I, Movar33/63; Class II, LVR140; Class III, V.Test and DN599) and restriction analysis of viral DNA revealed clear differences between the electrophoretic patterns of the four BHV-4 strains. The differentiation between BHV-4 strains can therefore be achieved by a biological method (mean plaque size) and by restriction analysis. The two genital isolates are easily differentiated by the two methods.
Dubuisson
J
J
Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Brussels, Belgium.
Thiry
E
E
Thalasso
F
F
Bublot
M
M
Pastoret
P P
PP
D000704:Analysis of Variance; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D003429:Cross Reactions; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D007700:Kinetics; D008297:Male; D011817:Rabbits; D010948:Viral Plaque Assay
D000914:Antibodies, Viral; D004279:DNA, Viral; D004262:DNA Restriction Enzymes