Permissive action of glucocorticoids on catecholamine-induced lipolysis: direct "in vitro" effects on the fat cell beta-adrenoreceptor-coupled-adenylate cyclase system.
Exposure of adipose tissue fragments to dexamethasone leads to enhanced lipolytic and cyclic AMP responses of isolated fat cells to isoproterenol. This permissive effect of the steroid is dose-dependent, prevented by the glucocorticoid receptor antagonist RU 38486, maximum after 48 h exposure to 10 nM dexamethasone and affects only the amplitude of the maximal response (+50%). Exposure to dexamethasone also induces an increase in both the number of beta-adrenergic receptors (+30%), and the adenylate cyclase-catalytic activity (+64%) and - responses to GTP (+114%) and isoproterenol (+55%). These data strongly suggest that the permissive effect of glucocorticoids towards lipolysis "in vivo" results at least in part from a glucocorticoid-receptor mediated action of these hormones on the fat cell membranous components involved in the beta-adrenergic control of lipolysis.
Lacasa
D
D
Department of Biochemistry, Faculty of Medecine Paris-Quest, C.H.I., Poissy, France.
Agli
B
B
Giudicelli
Y
Y
D000262:Adenylyl Cyclases / Q000502:physiology*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D003907:Dexamethasone / Q000494:pharmacology*; D004963:Estrenes / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000502:physiology; D007545:Isoproterenol / Q000494:pharmacology; D008053:Lipid Mobilization / Q000187:drug effects*; D008297:Male; D008345:Manganese / Q000494:pharmacology; D015735:Mifepristone; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D004963:Estrenes; D011943:Receptors, Adrenergic, beta; D005576:Colforsin; D015735:Mifepristone; D008345:Manganese; D003907:Dexamethasone; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D007545:Isoproterenol