Autogenous growth factor production by reticuloendotheliosis virus-transformed hematopoietic cells.
Reticuloendotheliosis virus strain T (REV-T)-transformed cells gave rise spontaneously to variants which secrete a factor that forms a distinct visible ring of precipitation (halo) surrounding colonies grown in soft agar. An Mr 15,000 protein was produced at higher levels by halo variants than by nonhalo-producing cells. An assay designed to detect the formation of precipitates enabled purification of an Mr 15,000 protein, p15, from serum-free medium conditioned by the growth of REV-T-transformed hematopoietic cells. Fractions enriched in p15 permitted the growth of REV-T-transformed cells under conditions where they normally failed to proliferate.
Garry
R F
RF
Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112.
Bose
H R
HR
D000818:Animals; D002461:Cell Line, Transformed / Q000378:metabolism; D006133:Growth Substances / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000378:metabolism* / Q000382:microbiology; D010641:Phenotype; D001356:Reticuloendotheliosis virus
D006133:Growth Substances