Identification of the human papillomavirus E2 protein in genital tract tissues.
A 27-kilodalton protein representing approximately 60% of the E2 open reading frame of human papillomavirus type 6 (HPV-6) was synthesized in a bacterial expression system. Affinity-purified polyclonal antibody to this protein detected the probable E2 gene product as a 50-kilodalton protein in most condylomas by Western blot (immunoblot) analysis. The E2-positive condylomas were associated with HPV-6, HPV-11, HPV-16, or unidentified HPVs.
Li
C C
CC
Program Resources, Inc., National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701.
Gilden
R V
RV
Showalter
S D
SD
Shah
K V
KV
D003218:Condylomata Acuminata / Q000382:microbiology*; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D005814:Genes, Viral; D005835:Genitalia / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay; D027383:Papillomaviridae / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics
D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins