title: Biochemical and genetic characterization of Corynebacterium pseudotuberculosis.
abstract: Isolates (n = 94) of Corynebacterium pseudotuberculosis were obtained from sheep, goats, horses, and cattle from various parts of the world. The isolates were characterized biochemically and by restriction endonuclease analysis of DNA. We found near homogeneity in the ability of isolates to ferment carbohydrates and to produce urease. All isolates produced phospholipase D and catalase. The ability of isolates from horses to reduce nitrate, the inability of isolates from sheep and goats to do so, and the correlation of this characteristic with results of restriction endonuclease analyses confirmed the existence of 2 biovars of C pseudotuberculosis. We propose that these biovars be referred to as biovar equi for isolates that reduce nitrate and biovar ovis for isolates that fail to do so.
authors.affiliation: Department of Veterinary Science, College of Agriculture, University of Arizona, Tucson 85721.
authors.forename: J G
authors.initials: JG
authors.lastname: Songer
authors.forename: K
authors.initials: K
authors.lastname: Beckenbach
authors.forename: M M
authors.initials: MM
authors.lastname: Marshall
authors.forename: G B
authors.initials: GB
authors.lastname: Olson
authors.forename: L
authors.initials: L
authors.lastname: Kelley
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000096:biosynthesis; D002417:Cattle; D003352:Corynebacterium / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D006041:Goats; D006736:Horses; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D010739:Phospholipase D / Q000096:biosynthesis; D012756:Sheep; D014510:Urease / Q000096:biosynthesis
chemical_list: D004269:DNA, Bacterial; D009566:Nitrates; D002374:Catalase; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D004262:DNA Restriction Enzymes; D010739:Phospholipase D; D014510:Urease