Elucidation of the mechanism responsible for the temperature-dependent reversible inactivation of the motility of fowl spermatozoa.
1. When washed fowl spermatozoa were held at 30 degrees C in a Ca++-free medium they retained internal Ca++, assimilated from the seminal plasma in vivo, which maintained their motility. 2. When this preparation was warmed to 40 degrees C, Ca++ was lost to the medium and the spermatozoa became immotile. 3. On subsequent cooling to 30 degrees C, the spermatozoa were capable of re-sequestering the Ca++, which restored their motility. 4. Removal of internal Ca++, with subsequent reduction of cellular activity, improved the survival of fowl spermatozoa held at 30 degrees C.
Thomson
M F
MF
Department of Molecular and Life Sciences, Dundee Institute of Technology, Scotland.
Wishart
G J
GJ
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002645:Chickens / Q000502:physiology*; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D013696:Temperature
D002118:Calcium