title: Morphologic study of inclusions in tissues from pigs inoculated with cytomegalovirus.
abstract: Six 5-day-old gnotobiotic pigs inoculated intranasally with the J-1 strain of porcine cytomegalovirus (PCMV) were euthanatized within 9 to 18 days after inoculation. All pigs developed a subclinical form of PCMV infection and had characteristic intranuclear inclusions in all tissues. Morphologically, 2 types of inclusions were observed. One was a small herpetic intranuclear inclusion in reticuloendothelial cells, which contained a few nucleocapsids in the nucleus. The other was a typical cytomegalic intranuclear inclusion in macrophages in the lungs and in tubuloalveolar gland cells in the nasal mucosa. Inclusions contained many nucleocapsides in the nucleus and a large number of enveloped viral particles in the cytoplasm. The distribution of the specific immunofluorescent antigen of PCMV was coincident with the presence of herpetic and cytomegalic inclusions.
authors.affiliation: Systematic Diagnosis Research Division, National Institute of Animal Health, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Narita
authors.forename: H
authors.initials: H
authors.lastname: Kawamura
authors.forename: J
authors.initials: J
authors.lastname: Shirai
authors.forename: M
authors.initials: M
authors.lastname: Haritani
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology*; D003586:Cytomegalovirus Infections / Q000473:pathology / Q000662:veterinary*; D005856:Germ-Free Life; D008854:Microscopy, Electron; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000473:pathology