Restriction maps of the regions coding for methicillin and tobramycin resistances on chromosomal DNA in methicillin-resistant staphylococci.
Chromosomal BamHI DNA fragments containing both the mecA gene encoding the penicillin-binding protein responsible for methicillin resistance and the aadD gene encoding 4',4"-adenylyltransferase responsible for tobramycin resistance were cloned from three methicillin- and tobramycin-resistant strains of Staphylococcus aureus and one strain of Staphylococcus epidermidis. Physical maps of the fragments were similar, suggesting their unique origin.
Ubukata
K
K
Department of Clinical Pathology, Teikyo University School of Medicine, Tokyo, Japan.
Nonoguchi
R
R
Matsuhashi
M
M
Song
M D
MD
Konno
M
M
D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D015245:Deoxyribonuclease BamHI / Q000235:genetics; D015247:Deoxyribonuclease HindIII / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D008712:Methicillin / Q000494:pharmacology*; D010403:Penicillin Resistance / Q000235:genetics; D010957:Plasmids; D015183:Restriction Mapping; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics; D014031:Tobramycin / Q000494:pharmacology*
D004269:DNA, Bacterial; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D008712:Methicillin; D014031:Tobramycin