An mRNA encoding poly-ubiquitin in porcine corpus luteum: identification by cDNA cloning and sequencing.
A cDNA library derived from a particular fraction of mRNA of porcine corpus luteum was screened by hybrid-release. cDNA clone pCL208 selected an mRNA which, in cell-free translation, directed the synthesis of a major polypeptide of 28 kD. Sequence analysis of clone pCL208 revealed that the entire coding sequence contained three complete repeats and a fourth incomplete repeat of porcine ubiquitin, joined head-to-tail without any spacer peptide sequences. The ultimate ubiquitin molecule contained a Phe residue as a carboxy-terminal extension. The coding sequence terminated with the stop codon TAA followed by a short untranslated region, the polyadenylation signal AATAAA, and a tract of A residues. Northern analysis indicated the presence of two types of ubiquitin-specific mRNAs, i.e., 1100 and 2500 bp, in porcine corpus luteum.
Einspanier
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Max-Planck-Institut für Biophysikaliśche Chemie, Abteilung Molekulare Biologie, Göttingen, West Germany.
Sharma
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HS
Scheit
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003338:Corpus Luteum / Q000502:physiology*; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005260:Female; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger; D013552:Swine; D014452:Ubiquitins / Q000235:genetics*
D011498:Protein Precursors; D012333:RNA, Messenger; D014452:Ubiquitins; D004247:DNA; D004262:DNA Restriction Enzymes