Changes in some histochemically demonstrable enzymes in macrophages exposed to quartz dust in vitro.
Cytochemical studies were carried out on rat alveolar and peritoneal macrophage cultures following exposure to quartz and corundum dusts. Quartz increased the number of ATPase positive cells and brought about an enhancement in the peroxidase and diffusion of the acid phosphatase activity of the exposed cells. In unexposed cell cultures, acid phosphatase activity was higher in alveolar than in peritoneal macrophages and was dependent upon the duration of incubation. Corundum produced no significant effect on the enzyme activity. Quartz treatment did not alter esterase activity whereas corundum exposed cultures showed a decline. A significant increase in mitochondrial succinic dehydrogenase activity was observed in peritoneal macrophages after quartz treatment. The results demonstrate alteration in the marker enzymes of plasma membranes, mitochondria and lysosomes during phagocytosis of quartz dust as the key event of dust cell interaction in vitro.
Dogra
S
S
Industrial Toxicology Research Centre, Lucknow, India.
Kaw
J L
JL
D000135:Acid Phosphatase / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004391:Dust / Q000009:adverse effects*; D004950:Esterases / Q000378:metabolism; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D010544:Peroxidases / Q000378:metabolism; D011791:Quartz / Q000633:toxicity*; D051381:Rats; D012822:Silicon Dioxide / Q000633:toxicity*
D004391:Dust; D011791:Quartz; D012822:Silicon Dioxide; D000255:Adenosine Triphosphate; D010544:Peroxidases; D004950:Esterases; D000135:Acid Phosphatase