title: Identification of a transposon Tn7-dependent DNA-binding activity that recognizes the ends of Tn7.
abstract: The bacterial transposon Tn7 is distinguished by its capacity for high-frequency transposition to a specific site in the Escherichia coli chromosome. tnsB is one of the five Tn7-encoded transposition genes. We have identified in vitro a tnsB-dependent DNA binding activity that interacts specifically with cis-acting transposition sequences at the Tn7 termini. Although the left and right termini of Tn7 are structurally distinct, each end contains several copies of a closely homologous 22-base-pair sequence. We present results indicating that this 22-base-pair repeat sequence is recognized by the tnsB-dependent binding activity.
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco 94143.
authors.forename: R L
authors.initials: RL
authors.lastname: McKown
authors.forename: C S
authors.initials: CS
authors.lastname: Waddell
authors.forename: L K
authors.initials: LK
authors.lastname: Arciszewska
authors.forename: N L
authors.initials: NL
authors.lastname: Craig
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins