[Heterologous system of immunoenzyme analysis for the diagnosis of human rotavirus infection and its comparison with RNA electrophoresis].
On the basis of porcine rotavirus a heterologous EIA test system was worked out and tested for diagnosis of human rotavirus infection. A high sensitivity and specificity of the test system was demonstrated, its results were compared with those of electron microscopy, diffuse precipitation test, and RNA electrophoresis. Out of 201 specimens (fecal filtrates) collected from children ranging in ages from 14 days to 10 years, rotavirus antigen was detected in 68 (33.8%) RNA electrophoresis demonstrated the rotaviruses circulating in Moscow to have basically the same electrophoretic type, although rotaviruses with other electrophoretic types, including the "short" electrophoretic type, were also detected.
Bukrinskaia
A G
AG
Rukhadze
G G
GG
Selivanov
Ia M
IaM
Molibog
E V
EV
Tentsov
Iu Iu
IuIu
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D007410:Intestinal Diseases / Q000175:diagnosis*; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000175:diagnosis*; D014776:Virus Cultivation
D000956:Antigens, Viral; D012367:RNA, Viral
Geterologichnaia sistema immunofermentnogo analiza dlia diagnostiki rotavirusnoĭ infektsii cheloveka i ee sopostavlenie s élektroforezom RNK.