High-performance liquid chromatography of glycosides on a new type of hydroxyapatite column.
High-performance liquid chromatography on a column of newly developed hard spherical hydroxyapatite was applied to the separation of a variety of glycosides of tri- and diterpenes including Ginseng saponins and Stevia sweet glycosides, affording satisfactory results by using a simple solvent system, aqueous acetonitrile. This normal phase chromatography is superior to chromatography on a silica gel column for the separation of water-soluble glycosides.
Kasai
R
R
Institute of Pharmaceutical Sciences, Hiroshima University, School of Medicine, Japan.
Yamaguchi
H
H
Tanaka
O
O
D002851:Chromatography, High Pressure Liquid; D017886:Durapatite; D006027:Glycosides / Q000302:isolation & purification*; D006882:Hydroxyapatites; D005894:Panax / Q000032:analysis; D010944:Plants / Q000032:analysis; D010946:Plants, Medicinal; D012503:Saponins / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D006027:Glycosides; D006882:Hydroxyapatites; D012503:Saponins; D017886:Durapatite