Persistent infection with herpes simplex virus type 1 in an Ia antigen-positive murine macrophage cell line.
The interaction of herpes simplex virus type 1 (HSV-1) with murine macrophage cell lines was examined. The cell lines appeared to be moderately permissive for HSV-1 replication, though the yield of the virus was limited compared with that in Vero cells. Furthermore, the murine macrophage cell line SL-1, bearing Ia antigen, was persistently infected with HSV-1 for over one year, and was designated SL-1/KOS. Persistent infection could not be established in an Ia antigen-negative macrophage cell line, SL-4. In the SL-1/KOS culture, there was a small number of infected cells as revealed by infectious center assay. Treatment with monoclonal antibody against HSV-1 cured the persistent infection. Therefore maintenance of the persistent infection is considered to be due to a carrier culture consisting of a minority of infected cells and a majority of uninfected cells. In the SL-1/KOS cultures a low level of interferon (IFN) was found. When a large amount of exogenous recombinant murine IFN-beta (10(5)-10(6) international units/ml) was added to the culture, virus production diminished to undetectable levels. These results suggest that IFN plays an important role in the maintenance of persistent infection. In long-term persistently infected cultures, syncytium formation appeared and the virus from such cultures had a different DNA structure from that of the virus originally used for infection as revealed by restriction endonuclease analysis.
Tang
J L
JL
Department of Virology, School of Medicine, Kyushu University, Fukuoka.
Yamamoto
M
M
Sakuma
S
S
Mori
R
R
Nagayama
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D000949:Histocompatibility Antigens Class II; D007370:Interferon Type I / Q000032:analysis / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D018139:Simplexvirus / Q000235:genetics / Q000502:physiology*; D013997:Time Factors; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D000949:Histocompatibility Antigens Class II; D007370:Interferon Type I