title: Enrichment of the spindle fiber attachments from mouse liver cell nuclei.
abstract: Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouse livers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and high speed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured by radioimmunoassay. The basic method offers opportunities for further increases of yield and for the enrichment of SFA of other vertebrates.
authors.affiliation: Shanghai Institute of Cell Biology, Academia Sinica.
authors.forename: L J
authors.initials: LJ
authors.lastname: Shi
authors.forename: Z M
authors.initials: ZM
authors.lastname: Ni
authors.forename: S F
authors.initials: SF
authors.lastname: Lu
authors.forename: G
authors.initials: G
authors.lastname: Wang
authors.forename: H L
authors.initials: HL
authors.lastname: Qi
authors.forename: Q X
authors.initials: QX
authors.lastname: Su
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000276:immunology; D015342:DNA Probes; D007074:Immunoglobulin G; D008099:Liver / Q000648:ultrastructure; D051379:Mice; D012595:Scleroderma, Systemic / Q000235:genetics*; D008941:Spindle Apparatus; D014162:Transfection
chemical_list: D015342:DNA Probes; D007074:Immunoglobulin G