Structural proteins of bovine parainfluenza-3 virus.
Six structural proteins of bovine parainfluenza-3 virus (PI-3V) labeled with [35S]-methionine could be resolved by polyacrylamide gel electrophoresis (PAGE). Five structural proteins of this virus had been previously reported. The 6 proteins found in this study were: L, a 180,000 (180 kD) molecular weight (MW) large protein; P, 83 kD phosphoprotein; HN, 69 kD hemagglutinin-neuraminidase glycoprotein; NP, 66 kD nucleocapsid protein; F, 55 kD fusion glycoprotein; and M, 38 kD matrix protein. Selective labeling with [2-3H]-mannose revealed only HN and F glycoprotein bands. A cellular actin protein (43 kD), associated with many enveloped viruses, was also found as a seventh protein in bovine PI-3V.
Panigrahi
P
P
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland Campus, College Park 20742.
Mohanty
S B
SB
Maheshwari
R K
RK
Friedman
R M
RM
D000818:Animals; D002213:Capsid / Q000032:analysis; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D007118:Immunoassay; D008970:Molecular Weight; D010224:Parainfluenza Virus 3, Human / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis; D018108:Respirovirus / Q000032:analysis*; D014758:Viral Core Proteins / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*; D015678:Viral Structural Proteins
D006023:Glycoproteins; D010750:Phosphoproteins; D014758:Viral Core Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins