Membrane-bound choline acetyltransferase is a cell surface marker of the differentiated NS-20Y cholinergic cell line.
When differentiated cells of the cholinergic NS-20Y type were incubated with an antiserum to choline acetyltransferase (ChAT), in the presence of complement, immunolysis occurred as demonstrated by release of 20% of total lactate dehydrogenase and 70% of total ChAT. No significant immunolytic effects were observed when either undifferentiated (dividing or non-dividing) or partially differentiated cells were incubated under identical conditions. When the Triton X-114 phase separation technique was employed using membranes from differentiated cells, a small but significant proportion of choline acetyltransferase was recovered in the detergent-rich phase. These results suggest that a membrane-bound form of ChAT is a surface marker of the NS-20Y cell line.
Barochovsky
O
O
Department of Biochemistry, Imperial College of Science and Technology, London, U.K.
Docherty
M
M
Bradford
H F
HF
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification*; D002454:Cell Differentiation; D002460:Cell Line; D002795:Choline O-Acetyltransferase / Q000302:isolation & purification*; D003165:Complement System Proteins / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification; D013574:Synaptosomes / Q000201:enzymology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology*
D000906:Antibodies; D000954:Antigens, Surface; D003165:Complement System Proteins; D007770:L-Lactate Dehydrogenase; D002795:Choline O-Acetyltransferase; D000254:Sodium-Potassium-Exchanging ATPase