Isolation of a cDNA encoding the muscle-specific subunit of human phosphoglycerate mutase.
We have isolated a full-length cDNA specifying the muscle-specific subunit of human phosphoglycerate mutase (PGAM-M). The cDNA encodes a deduced protein 253 amino acids in length and contains an unusually short (37 nucleotides) 3'-untranslated region. The deduced human PGAM-M protein is clearly related to yeast PGAM and to human diphosphoglycerate mutase. Genomic Southern analysis using the PGAM-M cDNA as a probe implies the presence of a large PGAM gene family in the human genome, while Northern analysis demonstrates tissue-specific transcription of this isoenzyme gene.
Shanske
S
S
H. Houston Merritt Clinical Research Center for Muscular Dystrophy and Related Disorders, Columbia University, College of Physicians & Surgeons, New York, New York 10032.
Sakoda
S
S
Hermodson
M A
MA
DiMauro
S
S
Schon
E A
EA
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D010736:Phosphoglycerate Mutase / Q000032:analysis / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*; D011487:Protein Conformation
D004247:DNA; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase