[Presence in human lymphocytes of a protein specifically binding to a cloned fragment of human satellite DNA III].
PHA-stimulated human lymphocytes contain the protein (SBP) which has selectivity in binding of 1.8 kb fragment of human satellite DNA III (HS3) as compared to other DNA sequences. It is shown that the binding site is localized within 1kb Sau3A-EcoR I fragment of HS3. SBP-binding activity is increased after treatment of cells with tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). The essential increase in a number of metaphases with chromosome endoreduplications in TPA-treated lymphocytes indicates that SBP may be involved in initiation of chromosome replication or in alteration of the mitotic spindle function.
Tomilin
N V
NV
Fedortseva
R F
RF
Svetlova
M P
MP
Podgornaia
O I
OI
D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D010957:Plasmids; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004276:DNA, Satellite; D004268:DNA-Binding Proteins; D004262:DNA Restriction Enzymes; D013755:Tetradecanoylphorbol Acetate
O prisutstvii v limfotsitakh cheloveka belka, spetsificheski sviazyvaiushchego klonirovannyĭ fragment satellitnoĭ DNK III cheloveka.