Changes in Na+-H+ exchange regulation upon granulocytic differentiation of HL60 cells.
The changes in activation of Na+-H+ exchange on granulocytic differentiation of human leukemic promyelocytic HL60 cells have been studied by measuring changes in intracellular pH with the fluorescent pH indicator 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF). It was found that the Na+-H+ exchanger is activated by stimulation of protein kinase C in the dimethyl sulfoxide (DMSO)-differentiated (neutrophil-like) HL60 cell, but not in the undifferentiated (promyelocyte-like) cell. In contrast, osmotic shrinkage of the cells triggers the exchanger in the undifferentiated cells, but not in the DMSO-differentiated cells. The data suggest that activation of Na+-H+ exchange by osmotic shock does not occur exclusively through stimulation of kinase C.
Restrepo
D
D
Department of Biophysics, University of Rochester Medical Center, New York 14642.
Kozody
D J
DJ
Knauf
P A
PA
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005452:Fluoresceins / Q000378:metabolism; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D019255:NADPH Oxidases; D011493:Protein Kinase C / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002352:Carrier Proteins; D005452:Fluoresceins; D017923:Sodium-Hydrogen Exchangers; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D004121:Dimethyl Sulfoxide