title: Use of A-549 cells in a clinical virology laboratory.
abstract: A-549 cells were compared with other cell lines for virus recovery, except from specimens submitted specifically for detection of cytomegalovirus. Of 589 specimens submitted specifically for detection of herpes simplex virus (HSV), 163 (28%) were positive for HSV--159 (97.5%) in A-549 cells and 156 (96%) in primary rabbit kidney cells. HSV cytopathic effect was identified an average of 0.6 day earlier in A-549 cells. Virus was recovered from 194 (11%) of 1,790 specimens submitted for general virus isolation. Of 40 HSV isolates, 85% were positive in A-549 cells, 72.5% were positive in MRC-5/WI-38 cells, and 42.5% were positive in HEp-2 cells. With adenovirus, 96% of 45 isolates were detected in A-549 cells, 62% were detected in HEp-2 cells, 38% were detected in MRC-5/WI-38 cells, and 31% were detected in PMK cells. Of the 76 enterovirus-positive specimens, 71% were positive in PMK cells, 62% were positive in A-549 cells, and 62% were positive in MRC-5/WI-38 cells. None of 12 respiratory syncytial virus, 14 rhinovirus, or 7 influenza A virus isolates were detected in A-549 cells. Of the cell lines examined, A-549 cells performed optimally for recovery of HSV and adenovirus, they allowed good growth of many of the enterovirus isolates, but they did not allow recovery of any of the respiratory syncytial virus, rhinovirus, or influenza A virus isolates.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68105.
authors.forename: G L
authors.initials: GL
authors.lastname: Woods
authors.forename: A
authors.initials: A
authors.lastname: Young
mesh_terms: D000260:Adenoviruses, Human / Q000254:growth & development / Q000302:isolation & purification*; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000254:growth & development / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008175:Lung Neoplasms; D017955:Poliovirus / Q000254:growth & development / Q000302:isolation & purification; D018139:Simplexvirus / Q000254:growth & development / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured; D014776:Virus Cultivation