Protective effect of the F(ab')2 fragments of monoclonal antibodies to mouse hepatitis virus.
The F(ab')2 fragments were prepared from three monoclonal antibodies (MAbs) reactive with either peplomer glycoprotein (E2) or nucleocapsid protein (NP) of a low-virulence mouse hepatitis virus (MHV), MHV-NuU. All the three MAbs could protect mice from challenge infection with virulent MHV-2, whereas only one of the anti-E2 MAbs was capable of neutralizing the virus in vitro. The F(ab')2 fragment of neutralizing anti-E2 MAb was shown to protect mice from challenge infection, but those of non-neutralizing anti-E2 and anti-NP MAbs were not protective.
Nakanaga
K
K
Department of Animal Pathology, University of Tokyo.
Yamanouchi
K
K
Fujiwara
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D002213:Capsid / Q000276:immunology; D005260:Female; D006524:Hepatitis, Viral, Animal / Q000276:immunology / Q000517:prevention & control*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D006517:Murine hepatitis virus / Q000276:immunology*; D009500:Neutralization Tests; D014758:Viral Core Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology; D015678:Viral Structural Proteins
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D014758:Viral Core Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins