Demonstration of high specificity antibodies against phosphatidylserine.
Anti-phosphatidylserine antibodies were raised in rabbits immunized with phosphatidylserine-polyacrylamide gels and with phosphatidylserine-cytochrome c vesicles. A solid-phase immunoenzymatic assay (ELISA) was developed to study the immune serum. The optimal conditions were defined and the technique used to obtain quantitative results. The anti-phosphatidylserine serum diluted to 1/1000 was tested against several phospholipids and was found to be highly specific to phosphatidylserine. This represents the first experimental demonstration of the specificity of antibodies raised against phosphatidylserine.
Maneta-Peyret
L
L
Laboratoire de Biogenèse membranaire, IBCN-CNRS, Bordeaux, France.
Bessoule
J J
JJ
Geffard
M
M
Cassagne
C
C
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000918:Antibody Specificity; D003574:Cytochrome c Group / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay; D010718:Phosphatidylserines / Q000008:administration & dosage / Q000276:immunology*; D010743:Phospholipids / Q000032:analysis; D011817:Rabbits
D000906:Antibodies; D003574:Cytochrome c Group; D010718:Phosphatidylserines; D010743:Phospholipids