title: Transposon Tn5 mutagenesis of Brucella abortus.
abstract: We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin. We have selected 22 independent kanamycin-resistant colonies in strain US-19 and 19 colonies in strain 2308. We have demonstrated by Southern hybridization that Tn5 was inserted into the chromosome.
authors.affiliation: Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
authors.forename: L D
authors.initials: LD
authors.lastname: Smith
authors.forename: F
authors.initials: F
authors.lastname: Heffron
mesh_terms: D002003:Brucella abortus / Q000235:genetics*; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D009154:Mutation; D010641:Phenotype
chemical_list: D004251:DNA Transposable Elements