title: Regulation of signal transfer from beta 1-adrenoceptor to adenylate cyclase by beta gamma subunits in a reconstituted system.
abstract: The beta gamma subunits of guanine nucleotide binding proteins from bovine brain and bovine rod outer segments have different structural and immunochemical properties. In spite of these structural differences, beta gamma subunits from these sources have been found to be fully interchangeable in terms of their interaction with alpha subunits of pertussis-toxin-sensitive G proteins. In contrast, however, there are striking differences between these beta gamma subunits with regard to their ability to deactivate fluoride-stimulated Gs. These profound differences were also observed when the interaction of the purified components of the adenylate cyclase system was studied after reconstitution into phospholipid vesicles. Addition of beta gamma purified from bovine brain to vesicles containing beta-receptor and Gs results in a biphasic effect on receptor-stimulated GTPase activity, whereas addition of transducin beta gamma was virtually without any effect. Likewise, beta gamma from bovine brain, but not transducin beta gamma, affected adenylate cyclase activity of a reconstituted system consisting of three purified components (R, Gs, C). Thus, the alpha subunit of Gs, but not the alpha subunits of pertussis-toxin-sensitive G proteins discriminate between structurally different beta gamma subunits.
authors.affiliation: Physiologisch-Chemisches Insititut der Universität Würzburg, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hekman
authors.forename: A
authors.initials: A
authors.lastname: Holzhöfer
authors.forename: P
authors.initials: P
authors.lastname: Gierschik
authors.forename: M J
authors.initials: MJ
authors.lastname: Im
authors.forename: K H
authors.initials: KH
authors.lastname: Jakobs
authors.forename: T
authors.initials: T
authors.lastname: Pfeuffer
authors.forename: E J
authors.initials: EJ
authors.lastname: Helmreich
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D004910:Erythrocyte Membrane / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D006706:Homeostasis; D007700:Kinetics; D037342:Pertussis Toxin; D010786:Photoreceptor Cells / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012374:Rod Cell Outer Segment / Q000378:metabolism*; D013873:Thionucleotides / Q000378:metabolism; D014422:Turkeys; D010566:Virulence Factors, Bordetella / Q000378:metabolism
chemical_list: D037361:Adenylate Cyclase Toxin; D011943:Receptors, Adrenergic, beta; D013873:Thionucleotides; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D037342:Pertussis Toxin; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases