Myeloperoxidase-oxidase oxidation of cysteamine.
Cysteamine oxidation was shown to be catalysed by nanomolar concentrations of myeloperoxidase in a peroxidase-oxidase reaction, i.e. an O2-consuming oxidation of a compound catalysed by peroxidase without H2O2 addition. When auto-oxidation of the thiol was prevented by the metal-ion chelator diethylenetriaminepenta-acetic acid, native, but not heat-inactivated, myeloperoxidase induced changes in the u.v.-light-absorption spectrum of cysteamine. These changes were consistent with disulphide (cystamine) formation. Concomitantly, O2 was consumed and superoxide radical anion formation could be detected by Nitro Blue Tetrazolium reduction. Both superoxide dismutase and catalase inhibited the reaction, whereas the hydroxyl-radical scavengers mannitol and ethanol did not. O2 consumption increased with increasing pH (between pH 6.0 and 8.0), and 50% inhibition was exhibited by about 3 mM-NaCl at pH 7.0 and by about 100 mM-NaCl at pH 8.0. Cysteamine was about 5 times as active (in terms of increased O2 consumption at pH 7.5) as the previously reported peroxidase-oxidase substrates NADPH, dihydroxyfumaric acid and indol-3-ylacetic acid. A possible reaction pathway for the myeloperoxidase-oxidase oxidation of cysteamine is discussed. These results indicate that cysteamine is a very useful substrate for studies on myeloperoxidase-oxidase activity.
Svensson
B E
BE
Research and Development Laboratories, Astra Alab AB, Södertälje, Sweden.
Lindvall
S
S
D002374:Catalase / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D003543:Cysteamine / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D009195:Peroxidase / Q000378:metabolism*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity; D013482:Superoxide Dismutase / Q000494:pharmacology
D002712:Chlorides; D006878:Hydroxides; D017665:Hydroxyl Radical; D003543:Cysteamine; D012973:Sodium Hypochlorite; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase