title: Transposon mutagenesis in Bordetella avium.
abstract: Transposon mutagenesis was used to introduce specific mutations in the chromosome of Bordetella avium. The transposon Tn5 was transferred by conjugation to B avium from Escherichia coli strain SM10 by the suicide plasmid vector pSUP1011. A defined minimal medium for growth of B avium was developed for scoring transposon-containing mutants for specific nutritional requirements. Approximately 1% of all mutants tested were auxotrophic in some form, and these organisms were grouped into 13 classes. Also, 2 classes of hemagglutination-negative mutants were isolated. Stability of Tn5 insertion was examined, and frequencies of reversion less than 10(-7) were observed, indicating stable mutations.
authors.affiliation: Department of Veterinary Microbiology, Iowa State University, Ames 50011.
authors.forename: R D
authors.initials: RD
authors.lastname: Leyh
authors.forename: R W
authors.initials: RW
authors.lastname: Griffith
authors.forename: L H
authors.initials: LH
authors.lastname: Arp
mesh_terms: D001884:Bordetella / Q000235:genetics*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial