Human monoclonal antibodies to Pseudomonas aeruginosa produced by EBV-transformed cells.
Numerous lymphoblastoid cell lines (LCLs) which secreted antibodies against Pseudomonas aeruginosa (all Fisher's immunotypes and Homma's immunotype 1) were established by Epstein-Barr virus (EBV)-transformation of lymphocytes. Five LCLs were established as long-term culture lines and their properties were determined. These LCLs produced monoclonal antibodies to Fisher's immunotype 1 and 4 and Homma's immunotype 1, and their immunoglobulin classes were IgM, IgG, and IgA. We found that three monoclonal antibodies (G3-1, H7-2, and E10-1) among them successfully protected mice from the corresponding immunotype of P. aeruginosa infection. Their protective dose (PD50) values were 0.5, 2.6, and 3.1 micrograms immunoglobulin/mouse. These human monoclonal antibodies against P. aeruginosa prepared by EBV-transformation method will be a valuable aid for the treatment for severe P. aeruginosa infections.
Suzuki
H
H
Central Research Laboratories, Wakunaga Pharmaceutical Co., Ltd., Hiroshima.
Okubo
Y
Y
Moriyama
M
M
Sasaki
M
M
Matsumoto
Y
Y
Hozumi
T
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000096:biosynthesis*; D002461:Cell Line, Transformed; D004854:Herpesvirus 4, Human; D006801:Humans; D008214:Lymphocytes; D051379:Mice; D008813:Mice, Inbred ICR; D011552:Pseudomonas Infections / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal