Fowlpox virus polypeptides: sequential appearance and virion associated polypeptides.
The polypeptides associated with fowlpox virus (FPV) infection of chicken embryo skin (CES) cells were examined by metabolic labelling with [35S]-methionine and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Polypeptide synthesis was followed over the first 48 hours post infection, as this was shown to be the period of viable virus production in CES cells. In contrast to infection with vaccinia virus (VV), which leads to a rapid total inhibition of host polypeptide synthesis in a number of cell lines, FPV infection of CES cells failed to cause a complete shut down of host polypeptide synthesis, with only a small number of host polypeptides being inhibited. A total of 21 FPV coded or induced polypeptides were resolved by metabolic labelling. As with VV, these polypeptides can be divided into two groups, the pre-replicative polypeptides containing a single member of 70,000 daltons, synthesised before viral DNA replication, and the post-replicative polypeptides, synthesised only after viral DNA replication has commenced. FPV DNA replication was shown to commence between 12 and 16 hours post-infection and to continue up to 48 hours post-infection. As also observed with VV, two temporally distinct classes of post-replicative polypeptides were identified based on their time of synthesis post-infection. The examination of purified FPV and VV by SDS-PAGE and coomassie blue staining allowed the resolution of 57 FPV particle associated polypeptides and 27 VV associated polypeptides.
Prideaux
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CT
Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra, A.C.T.
Boyle
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D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003588:Cytopathogenic Effect, Viral; D004261:DNA Replication; D004591:Electrophoresis, Polyacrylamide Gel; D005587:Fowlpox virus / Q000032:analysis* / Q000502:physiology; D011212:Poxviridae / Q000032:analysis*; D013997:Time Factors; D014616:Vaccinia virus / Q000502:physiology; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D014779:Virus Replication
D014764:Viral Proteins