Inhibition of superoxide anion production in guinea pig polymorphonuclear leukocytes by a seleno-organic compound, ebselen.
Production of superoxide anion (O2-) induced by tetradecanoyl phorbol acetate (TPA) in intact guinea pig polymorphonuclear leukocytes (PMNL) was markedly inhibited by a seleno-organic compound, 2-phenyl-1,2-benzisoselenazol-3(2H)-one (Ebselen), with glutathione peroxidase-like activity. The compound almost completely inhibited O2- production by a particulate fraction prepared from TPA-treated PMNL at a concentration as low as 250 nM.
Ichikawa
S
S
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Omura
K
K
Katayama
T
T
Okamura
N
N
Ohtsuka
T
T
Ishibashi
S
S
Masayasu
H
H
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D054833:Isoindoles; D009249:NADP / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D016566:Organoselenium Compounds; D012643:Selenium / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*
D000975:Antioxidants; D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; D013481:Superoxides; C042986:ebselen; D009249:NADP; D012643:Selenium