Positive selection of human cells lacking several transformation parameters from an SV40-transformed culture by means of parvovirus H-1.
The simian virus 40 (SV40)-transformed, newborn human kidney cell line NB-F was found to be heterogeneous with respect to its sensitivity to parvovirus H-1. The majority of the cells sustain a productive H-1 infection which eventually causes their lysis. Yet, a small fraction of the cells appears to be much less susceptible to H-1. Such a resistance to H-1 infection is a stable, transmissible property of this subpopulation of cells which was denoted NB-FR. The heterogeneity of NB-F cells is also apparent from the distribution of their karyotypes, which is bimodal and peaks at 114 and 46 chromosomes/cell. In contrast, the great majority of NB-FR cells contain 41-50 chromosomes. H-1-resistant and -sensitive cells appear to be related in several respects: they both contain morphologically human chromosomes as well as multiple SV40 DNA inserts, and could not be distinguished by isoenzyme typing. It was investigated whether the degree of sensitivity to H-1 infection correlated with other phenotypic properties of the human cell derivatives. NB-F cultures exhibit a series of transformation parameters, such as SV40 T-antigen expression, poor contact inhibition, clonogenicity in semi-solid medium and high lectin agglutinability, which are all much reduced or even undetectable in NB-FR cells. These observations suggest that cell susceptibility to H-1 segregates with marker(s) of in vitro malignant transformation. Moreover, the data indicate that parvoviruses can be used to preferentially remove transformants from a mixed culture of normal and transformed cells.
Su
Z Z
ZZ
Department of Biology, Fudan University, Shanghai, People's Republic of China.
Luo
Z Y
ZY
Guo
L P
LP
Dupont
F
F
Avalosse
B
B
Rommelaere
J
J
D000957:Antigens, Viral, Tumor / Q000032:analysis; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D010321:Parvoviridae / Q000502:physiology*; D010641:Phenotype; D013539:Simian virus 40 / Q000235:genetics
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral