title: Quantitative local cerebral blood flow measurements with technetium-99m HM-PAO: evaluation using multiple radionuclide digital quantitative autoradiography.
abstract: We investigated d,1 [99mTc]hexamethylpropyleneamine oxime complex (HM-PAO) as a tracer for quantitative measurement of local cerebral blood flow (LCBF) in a series of awake male rats. LCBF measurements with HM-PAO were compared to those of two other tracers, [14C] iodoantipyrine (IAP) and [201Tl]diethyldithiocarbamate (DDC), using quantitative double and triple tracer digital autoradiography. LCBF values with HM-PAO averaged 64% those of IAP and were generally linearly related. Detailed analysis suggested that the underestimation of LCBF by HM-PAO was related to blood constituent binding and/or rapid conversion to a noncerebrophilic compound, as well as noninstantaneous cerebral trapping, rather than to diffusion limitation.
authors.affiliation: Department of Radiology, University of Colorado Health Sciences Center.
authors.forename: J L
authors.initials: JL
authors.lastname: Lear
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives; D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging*; D002250:Carbon Radioisotopes; D002560:Cerebrovascular Circulation; D004050:Ditiocarb; D005069:Evaluation Studies as Topic; D007091:Image Processing, Computer-Assisted; D008297:Male; D009942:Organometallic Compounds; D010091:Oximes; D011877:Radionuclide Imaging; D051381:Rats; D013667:Technetium; D019690:Technetium Tc 99m Exametazime; D013794:Thallium Radioisotopes
chemical_list: D002250:Carbon Radioisotopes; D009942:Organometallic Compounds; D010091:Oximes; D013794:Thallium Radioisotopes; D019690:Technetium Tc 99m Exametazime; D013667:Technetium; D004050:Ditiocarb; D000983:Antipyrine; C008135:iodoantipyrine