Biphasic effects of dexamethasone on growth hormone release in vitro.
The effect of dexamethasone (Dex) on growth hormone (GH) release was examined in vitro in monolayer culture of normal rat pituitary cells and human somatotropinoma cells from patients with acromegaly. In either cell strain, Dex, at a concentration of 50 nM initially inhibited, but later (48 less than or equal to h) potentiated, the release of GH into the medium, with or without growth hormone releasing hormone (GHRH). The intracellular GH was significantly increased by 4-hour incubation with Dex in rat cell cultures. These results indicate a biphasic effect of glucocorticoids on GH release, irrespective of the origin of somatotrophs, and that the initial inhibitory effect is probably caused by inhibition of the release.
Nakagawa
K
K
Second Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Akikawa
K
K
Matsubara
M
M
Kubo
M
M
Ishizuka
T
T
Obara
T
T
D000172:Acromegaly / Q000378:metabolism; D000239:Adenoma, Acidophil / Q000378:metabolism; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D006801:Humans; D010902:Pituitary Gland / Q000187:drug effects; D010911:Pituitary Neoplasms / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D003907:Dexamethasone; D013006:Growth Hormone