title: An enzymatic assay for picomole levels of phosphatidate.
abstract: An assay for phosphatidate that is sensitive, specific, and highly reproducible is described. Phosphatidate is transacylated with methylamine and the glycerol 3-phosphate produced is measured enzymatically. The assay is linear from 50 to 1500 pmol of phosphatidate and can be used to quantitate phosphatidate in small amounts of biological materials.
authors.affiliation: Howard Hughes Medical Institute, Nashville, Tennessee.
authors.forename: S B
authors.initials: SB
authors.lastname: Bocckino
authors.forename: P
authors.initials: P
authors.lastname: Wilson
authors.forename: J H
authors.initials: JH
authors.lastname: Exton
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D005993:Glycerolphosphate Dehydrogenase; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis / Q000166:cytology; D008297:Male; D008744:Methylamines; D008832:Microchemistry; D009243:NAD; D010712:Phosphatidic Acids / Q000032:analysis*; D010759:Phosphorus Isotopes; D051381:Rats
chemical_list: D008744:Methylamines; D010712:Phosphatidic Acids; D010759:Phosphorus Isotopes; D009243:NAD; C027451:methylamine; D005993:Glycerolphosphate Dehydrogenase; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+)