title: Studies on regenerating liver and hepatoma plasma membranes--II. Membrane fluidity and enzyme activity.
abstract: 1. Rat hepatocyte plasma membranes isolated from Morris hepatoma 7288C, normal and regenerating liver were labelled with the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene. 2. Steady-state fluorescence polarisation measurements indicated an increased fluidity of the membranes in the early stages of regeneration, returning to normal levels after 48 hr. 3. There was a decrease in hepatoma plasma membrane fluidity compared to normal hepatocytes. Changes in fluorescence polarisation with temperature (Arrhenius studies) indicate an increase in the lower critical temperature for the membrane lipid thermotropic transition of hepatoma compared to normal liver plasma membranes. 4. These changes in membrane lipid fluidity alter the activation of some intrinsic and extrinsic membrane bound enzymes.
authors.affiliation: Department of Biochemistry, University of Queensland, St Lucia, Australia.
authors.forename: S M
authors.initials: SM
authors.lastname: Mahler
authors.forename: P A
authors.initials: PA
authors.lastname: Wilce
authors.forename: B C
authors.initials: BC
authors.lastname: Shanley
mesh_terms: D015720:5'-Nucleotidase; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004161:Diphenylhexatriene; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D008560:Membrane Fluidity; D009708:Nucleotidases / Q000378:metabolism*; D051381:Rats; D011915:Rats, Inbred BUF; D012016:Reference Values; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D004161:Diphenylhexatriene; D000242:Cyclic AMP; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D000262:Adenylyl Cyclases