A dominant selectable marker for the construction of recombinant poxviruses.
Mycophenolic acid has been shown to be a potent inhibitor of vaccinia virus growth. By inserting the Escherichia coli xanthine-guanine phosphoribosyl transferase gene (gpt) into the vaccinia virus genome under control of the P-7.5 promoter this inhibition was overcome. When coupled in tandem with another gene of interest, recombinant vaccinia viruses can be positively selected carrying both genes. Since the gpt gene operates as a selectable marker in most mammalian cells it will be useful as a dominant selectable marker for the construction of recombinant viruses based on other host-specific poxviruses.
Boyle
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DB
Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory, Geelong, Victoria.
Coupar
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BE
D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005814:Genes, Viral; D009173:Mycophenolic Acid / Q000494:pharmacology*; D010430:Pentosyltransferases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014616:Vaccinia virus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase; D004262:DNA Restriction Enzymes; D009173:Mycophenolic Acid