Production of antibodies of predetermined specificity against herpes simplex virus DNA polymerase and their use in characterization of the enzyme.
Peptides from preselected regions of the herpes simplex virus DNA polymerase were used to generate monospecific antisera to defined regions of the enzyme. The antisera were used to localize the polymerase within the infected cell and to determine the time of synthesis during productive infection. Comparison with a neutralizing polyclonal antiserum was used to show the specificity of the peptide antisera. By using the antisera the stabilities of the DNA polymerase, the alkaline nuclease, and the major DNA-binding protein were determined, and the state of phosphorylation of the DNA polymerase was compared with each of these proteins.
Thomas
M S
MS
Department of Biochemical Virology, Wellcome Research Laboratories, Beckenham, Kent, United Kingdom.
Banks
L M
LM
Purifoy
D J
DJ
Powell
K L
KL
D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000918:Antibody Specificity; D004259:DNA-Directed DNA Polymerase / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology; D010766:Phosphorylation; D018139:Simplexvirus / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D004259:DNA-Directed DNA Polymerase