Immunophenotyping of acute myeloid leukemia by immuno-alkaline phosphatase (APAAP) labeling with a panel of antibodies.
A panel of eight antibodies was used by the alkaline-phosphatase/anti-alkaline phosphatase (APAAP) method to stain peripheral blood films, bone marrow smears, and cytocentrifuge preparations from 29 cases of acute myeloid leukemia. These findings were correlated with the French-American-British (FAB) classification. Leukemic cells from six cases of myeloblastic leukemia (FAB;M1) were predominantly labeled by the antimyeloperoxidase monoclonal antibody (MPO-7). Leukemic cells from the majority of eight cases of myeloblastic leukemia with maturation (FAB;M2) and progranulocytic leukemia (FAB;M3) stained with monoclonal antibodies MPO-7, NP57 (anti-elastase), and EBM11 (antimonocyte/macrophage). Leukemic cells from six cases of myelomonocytic (FAB;M4) and five cases of monocytic (FAB;M5) leukemia were most often labeled with antibodies MPO-7, NP57, and EBM11 as well as monoclonal antibodies Y1/82A (anti-monocyte) and KB90 (against the p150, 95 molecule, CD11c; a monocyte/granulocyte marker), but not with monoclonal antibody C17 (antiglycoprotein IIb/IIIa) and/or monoclonal antibody Y2/51 (antiglycoprotein IIIa). Erythroblasts from a single case of erythroleukemia (FAB;M6) were not labeled with any of the antibodies from this panel. Leukemic cells from two cases of acute megakaryocytic leukemia (FAB;M7) stained strongly with the monoclonal antiglycoprotein IIIa/IIb antibody (C17) and antiglycoprotein IIIa antibody (Y2/51). Staining by the APAAP method with this panel of antibodies was easy to perform, required no expensive instrumentation, and provided useful information in the classification of acute myeloid leukemia.
Davey
F R
FR
Department of Pathology, SUNY Health Science Center, Syracuse 13210.
Erber
W N
WN
Gatter
K C
KC
Mason
D Y
DY
D000469:Alkaline Phosphatase / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006651:Histocytochemistry; D006801:Humans; D007781:Lactoferrin / Q000032:analysis; D015470:Leukemia, Myeloid, Acute / Q000145:classification* / Q000201:enzymology / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D009195:Peroxidase / Q000097:blood; D010641:Phenotype; D010980:Platelet Membrane Glycoproteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D010980:Platelet Membrane Glycoproteins; D009195:Peroxidase; D000469:Alkaline Phosphatase; D007781:Lactoferrin