The development and standardization of an enzyme-linked immunosorbent assay for the detection of antibodies to bovine herpesvirus 2.
A single dilution enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bovine herpesvirus 2 has been developed, standardized and compared with the virus neutralization test. The results of the two tests correlated well. A positive/negative threshold was established for the ELISA. The ELISA was reproducible, sensitive, rapid and specific.
Bushnell
S E
SE
Central Veterinary Laboratory, Ministry of Agriculture, Fisheries and Food, Weybridge, Surrey, UK.
Edwards
S
S
D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006564:Herpesviridae / Q000276:immunology*; D001911:Herpesvirus 2, Bovine / Q000276:immunology*; D009500:Neutralization Tests
D000914:Antibodies, Viral