T-cell-mediated inhibition of EBV-induced B-cell transformation: recognition of virus particles.
In order to analyze the components of EBV-specific immunological memory that contribute to the T-cell-mediated inhibition of EBV-induced B-cell transformation, we have investigated the growth inhibitory potential of T cells cultured for 3 days with B cells exposed to transforming and non-transforming EBV preparations. T cells cultured for 3 days with EBV-infected autologous B cells inhibited the transformation of newly infected B lymphocytes. T cells cultured with the virus, with autologous uninfected B cells, or in the presence of 10 micrograms/ml Con-A had a significantly weaker inhibitory capacity. The inhibitory capacity was induced by co-cultivation with B cells infected with the transforming B95-8 virus strain, with the non-transforming P3HRI virus strain, and also with UV-inactivated B95-8 virus. B-cells infected with transforming B95-8 virus induced the strongest growth inhibitory activity. Activation of the T cells was shown by their IL-2 production, proliferation, and generation of non-specific cytotoxicity. These results suggest that the initial event of the T-cell response in EBV-infected cultures is induced by antigens associated with the viral particles presented by B lymphocytes.
Bejarano
M T
MT
Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
Masucci
M G
MG
Klein
G
G
Klein
E
E
D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000382:microbiology*; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000502:physiology*
D000956:Antigens, Viral; D008222:Lymphokines