Recent observations on the structure and the properties of yeast NMN adenylyltransferase.
A homogeneous preparation of yeast NMN adenylyltransferase (EC 2.7.7.1) showed microheterogeneity, which was revealed by FPLC (Fast Protein Liquid Chromatography) ion exchange chromatography. The resolved components have been characterized with respect to electrophoretic behavior and adenine content. The results led to a hypothesis about a possible role of poly(ADP-ribosylation) in modulating the enzyme activity.
Ruggieri
S
S
Dipartimento di Biologia Cellulare, Università di Camerino, Italy.
Gregori
L
L
Natalini
P
P
Vita
A
A
Magni
G
G
D000225:Adenine / Q000032:analysis; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D009612:Nicotinamide-Nucleotide Adenylyltransferase / Q000032:analysis / Q000302:isolation & purification*; D009713:Nucleotidyltransferases / Q000302:isolation & purification*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D011064:Poly Adenosine Diphosphate Ribose; D009713:Nucleotidyltransferases; D009612:Nicotinamide-Nucleotide Adenylyltransferase; D000225:Adenine