title: Cross-hybridization and relationships of various papillomavirus DNAs at different degrees of stringency.
abstract: Cloned DNAs of 21 different papillomaviruses which naturally infect mammals and one bird papillomavirus were compared for relative homology by Southern blot hybridization. Blots were carried out under low (Tm-40 degrees), medium (Tm-33 degrees), and high (Tm-22 degrees) stringency conditions. At higher stringency, human papillomaviruses cross-hybridized with each other reflecting species-specific similarities. Bovine papillomavirus types 1, 2, 5, European elk papillomavirus, and deer papillomavirus also cross-hybridized at higher stringencies probably reflecting the association of these viruses with fibroblast-prolific lesions. The hybridization data presented here may be useful in future classification attempts. They are also useful as a guide in the selection of papillomavirus DNA probes for analysis of extracts from warts and tumors.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana 61801.
authors.forename: M K
authors.initials: MK
authors.lastname: O'Banion
authors.forename: J P
authors.initials: JP
authors.lastname: Sundberg
authors.forename: A A
authors.initials: AA
authors.lastname: Reszka
authors.forename: M E
authors.initials: ME
authors.lastname: Reichmann
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
chemical_list: D004279:DNA, Viral