title: Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes.
abstract: Detection of viral DNA in infected tissues is demonstrated by in situ nucleic acid hybridization employing bromodeoxyuridine (BrdUrd)-labeled probes. BrdUrd is used alternatively to biotinylated nucleotides in the preparation of DNA probes and, after hybridization, developed with a monoclonal anti-BrdUrd antibody and the alkaline phosphatase-anti-alkaline phosphatase technique. The sensitivity using both BrdUrd- and biotin-labeled probes is similar. Furthermore, the simultaneous detection of viral coinfections will be possible by means of this technique in conjunction with biotin-labeled DNA probes.
authors.affiliation: Institute for Pathology, Freie Universität Berlin, West Germany.
authors.forename: G
authors.initials: G
authors.lastname: Niedobitek
authors.forename: T
authors.initials: T
authors.lastname: Finn
authors.forename: H
authors.initials: H
authors.lastname: Herbst
authors.forename: G
authors.initials: G
authors.lastname: Bornhöft
authors.forename: J
authors.initials: J
authors.lastname: Gerdes
authors.forename: H
authors.initials: H
authors.lastname: Stein
mesh_terms: D001973:Bromodeoxyuridine; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000473:pathology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008168:Lung / Q000382:microbiology* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D010957:Plasmids
chemical_list: D004279:DNA, Viral; D001973:Bromodeoxyuridine