title: Properties of simian virus 40 mutants lacking the Asp4-Glu-Asp stretch at the carboxyl-terminus of large T antigen.
abstract: The biological activity carried by the carboxy-terminal domain of SV40 large T antigen has been investigated by isolating mutants deleted for a stretch of six acidic residues which by analogy with polyoma middle T antigen might be essential for the activity of the protein. We have constructed an "in-phase" deletion of 37 residues that includes the complete acid residues cluster. In order to parallel the polyoma hr-t mutants genotype, the deletion was introduced in virus strains either competent or defective for the small t antigen. We conclude from these experiments that the deletion of this unusual sequence does not affect per se any of the known biological properties of the virus.
authors.affiliation: Laboratorie d'Oncologie Moléculaire, Institut Gustave Roussy, Villejuif, France.
authors.forename: F
authors.initials: F
authors.lastname: Pecceu
authors.forename: A
authors.initials: A
authors.lastname: Komly
authors.forename: M
authors.initials: M
authors.lastname: Gardes
authors.forename: J
authors.initials: J
authors.lastname: Feunteun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D001483:Base Sequence; D002472:Cell Transformation, Viral; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D013329:Structure-Activity Relationship; D014779:Virus Replication
chemical_list: D000957:Antigens, Viral, Tumor