title: Lonidamine-induced outer membrane permeability and susceptibility of mitochondria to inhibition by adriamycin.
abstract: Low concentrations of Adriamycin (ADM) do not inhibit the oxygen consumption of rat liver mitochondria because of the inability to cross the outer membrane. The involvement of this membrane as a permeability barrier is demonstrated by the results with exogenous ferrocytochrome c. ADM does not affect the basal rate of ferrocytochrome c oxidation, which, on the contrary, increases in Lonidamine (LND)-treated mitochondria. This difference lies in the capacity of LND to unmask the redox carriers in the inner membrane, i.e. cytochrome c:oxygen oxidoreductase which is also a marker of the outer membrane permeabilization. Therefore, the enhancement of ADM's effect on mitochondrial respiration by LND must be ascribed to its permeabilizing effect on the outer mitochondrial membrane.
authors.affiliation: Regina Elena Institute for Cancer Research, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Floridi
authors.forename: C
authors.initials: C
authors.lastname: Bianchi
authors.forename: A
authors.initials: A
authors.lastname: Bagnato
authors.forename: A
authors.initials: A
authors.lastname: Gambacurta
authors.forename: M G
authors.initials: MG
authors.lastname: Paggi
authors.forename: B
authors.initials: B
authors.lastname: Silvestrini
authors.forename: A
authors.initials: A
authors.lastname: Caputo
mesh_terms: D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D004347:Drug Interactions; D007191:Indazoles / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000187:drug effects* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D010539:Permeability; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats
chemical_list: D003574:Cytochrome c Group; D007191:Indazoles; D011720:Pyrazoles; D004317:Doxorubicin; C016371:lonidamine