title: Cloning and expression of full-length cDNA encoding human vitamin D receptor.
abstract: Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of approximately equal to 4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.
authors.affiliation: California Biotechnology Inc., Mountain View 94043.
authors.forename: A R
authors.initials: AR
authors.lastname: Baker
authors.forename: D P
authors.initials: DP
authors.lastname: McDonnell
authors.forename: M
authors.initials: M
authors.lastname: Hughes
authors.forename: T M
authors.initials: TM
authors.lastname: Crisp
authors.forename: D J
authors.initials: DJ
authors.lastname: Mangelsdorf
authors.forename: M R
authors.initials: MR
authors.lastname: Haussler
authors.forename: J W
authors.initials: JW
authors.lastname: Pike
authors.forename: J
authors.initials: J
authors.lastname: Shine
authors.forename: B W
authors.initials: BW
authors.lastname: O'Malley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D011994:Recombinant Proteins; D004247:DNA; D004262:DNA Restriction Enzymes