Cerebral ischemia decreases endogenous calcium-dependent protein phosphorylation in gerbil brain.
Acute forebrain ischemia in the gerbil produced a significant decrease in endogenous Ca2+-dependent hippocampal protein phosphorylation in comparison to sham and naive control animals. Inhibition of Ca2+-dependent phosphorylation was demonstrated as early as 2 h post-ischemia and shown to persist up to 7 days following the ischemic insult. The ischemia-induced loss of Ca2+-dependent phosphorylation was only seen in brain regions affected by the localized ischemia and not in non-ischemic brain regions from the same animal. These results suggest that an early event in the development of ischemia-induced neuronal death is a decrease in Ca2+-dependent protein phosphorylation.
Taft
W C
WC
Department of Neurology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Tennes-Rees
K A
KA
Blair
R E
RE
Clifton
G L
GL
DeLorenzo
R J
RJ
D000818:Animals; D001921:Brain / Q000201:enzymology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005849:Gerbillinae; D002546:Ischemic Attack, Transient / Q000201:enzymology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009928:Organ Specificity; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D012016:Reference Values
D009419:Nerve Tissue Proteins; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases