The preparation and spectroscopic characterization of a weakly self-associating salt of guanylyl-(3'-5')-guanosine.
The tetramethylammonium salt of guanylyl-(3'-5')-guanosine has been prepared by a cation-exchange technique and it has been found that the tetramethylammonium ion drastically reduces the self-association of GpG in solution. This has allowed the characterization of GpG by FTIR and 1-D and 2-D NMR spectroscopy. A complete, well-resolved 1H NMR spectrum in D2O has been obtained and all resonances have been assigned. A weak, essentially non-cooperative intermolecular association is observed in solution (15-20 mM) below 40 degrees C. The association occurs via base stacking and base-base hydrogen bonding.
Toth
C R
CR
Department of Chemistry, University of Toledo, Ohio 43606.
Walmsley
J A
JA
D055598:Chemical Phenomena; D002621:Chemistry; D015226:Dinucleoside Phosphates; D006150:Guanine Nucleotides / Q000032:analysis*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D000644:Quaternary Ammonium Compounds; D012492:Salts; D013055:Spectrophotometry, Infrared; D013696:Temperature
D015226:Dinucleoside Phosphates; D006150:Guanine Nucleotides; D000644:Quaternary Ammonium Compounds; D012492:Salts; D006151:Guanosine; C018309:guanylyl-(3'-5')-guanosine; D006157:Guanosine Monophosphate; C027917:tetramethylammonium