title: Vesicular stomatitis virus in Drosophila melanogaster cells: regulation of viral transcription and replication.
abstract: Vesicular stomatitis virus RNA synthesis was investigated during the establishment of persistent infection in Drosophila melanogaster cells. The transcription rate declined as early as 5 h after infection and was strongly inhibited after 7 h, leading to a decrease in viral mRNA levels and in viral protein synthesis rates. Full-length plus-strand antigenomes and minus-strand genomes were detected after a 3-h lag time and accumulated until 15 h after infection. Short encapsidated plus-strand molecules were also generated corresponding to the 5' end of viral defective antigenomes. Assembly and release of virions were not restricted, but their infectivity was extremely reduced. In persistently infected cells, an equilibrium was reached where the level of intracellular genomes maintained was constant and maximal even after the rate of all viral syntheses had decreased. These results are discussed with regard to the establishment of persistent infection.
authors.affiliation: Laboratoire de Génétique des Virus, Centre National de la Recherche Scientifique, Gif sur Yvette, France.
authors.forename: D
authors.initials: D
authors.lastname: Blondel
authors.forename: A M
authors.initials: AM
authors.lastname: Petitjean
authors.forename: S
authors.initials: S
authors.lastname: Dezélée
authors.forename: F
authors.initials: F
authors.lastname: Wyers
mesh_terms: D000818:Animals; D002460:Cell Line; D004331:Drosophila melanogaster / Q000382:microbiology*; D005786:Gene Expression Regulation; D007700:Kinetics; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics* / Q000254:growth & development; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins