title: Effect of high salt intake on sodium, potassium-dependent adenosine triphosphatase activity in the erythrocytes of normotensive men.
abstract: 1. We measured ouabain-insensitive adenosine triphosphatase (ATPase), sodium, potassium-dependent adenosine triphosphatase (Na+,K+-ATPase) and intracellular Na+ and K+ in the erythrocytes of 19 healthy volunteers, before and after supplementation of their normal diet was 6.0-8.9 g of salt (102-137 mmol of NaCl) per day, for 5 days. 2. The subjects had a small but significant gain in weight. Mean plasma renin activity decreased from 1.57 to 0.73 pmol of angiotensin 1 h-1 ml-1 and plasma aldosterone from 0.46 to 0.24 nmol/l. 3. Total ATPase activity fell from 197.9 nmol of inorganic phosphate h-1 mg-1 during the control period to 173.5 during the high-salt period (P less than 0.0125). Na+, K+-ATPase activity fell from 162.2 to 141.4 nmol of inorganic phosphate h-1 mg-1 (P less than 0.05). Intracellular Na+ and intracellular K+ did not change. 4. These results are consistent with the hypothesis that salt-induced volume expansion causes the release of a factor inhibitory to the Na+ pump.
authors.affiliation: Section of Nephrology/Hypertension, Veterans Administration Lakeside Medical Center, Chicago, IL 60611.
authors.forename: A P
authors.initials: AP
authors.lastname: Quintanilla
authors.forename: M I
authors.initials: MI
authors.lastname: Weffer
authors.forename: H
authors.initials: H
authors.lastname: Koh
authors.forename: M
authors.initials: M
authors.lastname: Rahman
authors.forename: A
authors.initials: A
authors.lastname: Molteni
authors.forename: F
authors.initials: F
authors.lastname: del Greco
mesh_terms: D000328:Adult; D000450:Aldosterone / Q000097:blood; D001794:Blood Pressure; D001835:Body Weight; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D008297:Male; D011187:Posture; D011188:Potassium / Q000097:blood; D012083:Renin / Q000097:blood; D012964:Sodium / Q000097:blood; D012982:Sodium, Dietary / Q000008:administration & dosage / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
chemical_list: D012982:Sodium, Dietary; D000450:Aldosterone; D012964:Sodium; D012083:Renin; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium