[Effect of berberine on slow inward ionic current in guinea pig ventricular papillary muscle].
Using a single sucrose gap voltage clamp technique, it was found that berberine hydrochloride (Ber-HCl) had a dose-dependent biphasic effect on Isi of guinea pig papillary muscle. It was more difficult to wash out the inhibitory effect of Ber-HCl on Isi. In the initial stage of the experiment, Ber-HCl 72 mumol/L and 365 mumol/L greatly improved the influx of extracellular Ca2+ and increased the amplitude of Isi. As the drug perfusion was prolonged, however, Ber-HCl dramatically blocked Ca2+ channels and decreased the influx of extracellular Ca2+ consequently, Isi was reduced in amplitude and later disappeared. This experiment further revealed the mechanisms of action of Ber on cardiac contractility and for the treatment of arrhythmias.
Sun
X D
XD
Li
J M
JM
Tian
L J
LJ
Wang
Y P
YP
Yu
Y F
YF
Zhang
K Y
KY
D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D001600:Berberine Alkaloids / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers; D004594:Electrophysiology; D005260:Female; D006168:Guinea Pigs; D008297:Male; D010210:Papillary Muscles / Q000187:drug effects / Q000502:physiology
D001600:Berberine Alkaloids; D002121:Calcium Channel Blockers; D001599:Berberine; D002118:Calcium