title: [Effective selection and analysis of IS1 element transposition using the oLpLN region of phage lambda].
abstract: The plasmid pNT6 permits selection of IS1-element insertions into the plasmid occurring with the frequency about 10%. The bacteriophage promoter pL cloned in pNT6 is a hot-spot region for IS1-element insertion. The frequency of IS1 transposition into pL depends on genotype. The plasmid pNT6 may be considered to be a useful target DNA for screening and analysis of IS1-element transposition.
authors.forename: I L
authors.initials: IL
authors.lastname: Glukhov
authors.forename: I
authors.initials: I
authors.lastname: Fodor
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014169:Transformation, Bacterial
chemical_list: D004251:DNA Transposable Elements
vernacular_title: Effektivnyĭ otbor i analiz transpozitsiĭ IS1-élementa s pomoshch'iu oblasti oLpLN faga lambda.