Capsid protein VP1 (p85) of Aleutian disease virus is a major DNA-binding protein.
The interaction between Aleutian disease virus (ADV) DNA and proteins isolated from ADV-infected cells or ADV virions, respectively, was examined. Proteins were separated on SDS-polyacrylamide gels, transferred to nitrocellulose, and probed with 32P-labeled restriction fragments of replicative form (RF) DNA or with single-stranded virion DNA. The ADV capsid protein VP1 was found to bind the 3'-terminal BamHI fragment of RF DNA extending from map units (m.u.) 0 to 15. No binding was observed with the fragment extending from m.u. 15 to 63 and only minor amounts of label were detected with the 5'-terminal EcoRV fragment extending from m.u. 63 to 100. With the latter fragment, small amounts of label were also detected bound to higher-molecular-weight proteins of about 100,000 Da. No binding of DNA to the ADV nonstructural protein NS1 or to the ADV capsid protein VP2 was detected. Additionally, single-stranded virion DNA was found to bind to VP1.
Willwand
K
K
Institute of Virology, Hannover Veterinary School, Federal Republic of Germany.
Kaaden
O R
OR
D000454:Aleutian Mink Disease Virus / Q000235:genetics / Q000648:ultrastructure*; D002213:Capsid / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000502:physiology*; D008970:Molecular Weight; D010321:Parvoviridae / Q000648:ultrastructure*; D011485:Protein Binding
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins