Detection of free radical formation in various tissues after acute carbon tetrachloride administration in gerbil.
alpha-phenyl N-tert-butyl nitrone (PBN) was used as a spin-trapping agent to search for free radical formation in various tissues of gerbils. A significantly large amount of free radicals was detected in liver, kidney, heart, lung and testis after a single intraperitoneal dose of CCl4. We have also detected free radical formation in the brain and blood, although the number of spins was considerably smaller than those found in the other tissues. Free radicals formed in brain tissue after an oxidative insult may give strong supporting evidence for the free radical theory of aging.
Ahmad
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Sinclair Comparative Medicine Research Farm, University of Missouri, Columbia 65203.
Cowan
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Sun
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AY
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism / Q000473:pathology*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D005849:Gerbillinae; D008297:Male; D009928:Organ Specificity
D005609:Free Radicals