title: Detection by 125I-cationized cytochrome c of proteoglycans and glycosaminoglycans immobilized on unmodified and on positively charged nylon 66.
abstract: We have examined the detection by a 125I-labeled basic protein, cationized cytochrome c, of selected proteoglycans (PGs) and standard preparations of glycosaminoglycans (GAGs) immobilized on Nylon 66 and also on positively charged Nylon 66. Immobilization on Nylon 66 appears to allow a relative freedom of interaction between PGs or GAGs and 125I-cationized cytochrome c, but a more restricted reaction was observed when PGs and GAGs were immobilized to positively charged Nylon 66. On this support PGs with large numbers of GAG side chains reacted well with 125I-cationized cytochrome c, but GAGs were minimally reactive. By taking advantage of some of the properties of large-pore agarose-acrylamide gels, rapid partial characterization of some PGs can be accomplished in the 10-ng range, and therefore at a sensitivity equal to PGs with internal biosynthetic labels.
authors.affiliation: Department of Biochemistry, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
authors.forename: R
authors.initials: R
authors.lastname: Heimer
authors.forename: L
authors.initials: L
authors.lastname: Molinaro
authors.forename: P M
authors.initials: PM
authors.lastname: Sampson
mesh_terms: D000818:Animals; D001011:Aorta / Q000032:analysis; D002417:Cattle; D003574:Cytochrome c Group; D006025:Glycosaminoglycans / Q000032:analysis*; D006132:Growth Plate / Q000032:analysis; D007457:Iodine Radioisotopes; D009757:Nylons; D011485:Protein Binding; D011509:Proteoglycans / Q000032:analysis*; D011865:Radioisotope Dilution Technique
chemical_list: D003574:Cytochrome c Group; D006025:Glycosaminoglycans; D007457:Iodine Radioisotopes; D009757:Nylons; D011509:Proteoglycans