title: [Isolation and characteristics of clones complementary to mRNA of the murine cellular tumor antigen p53].
abstract: 43 cDNA clones specific for murine cellular tumor antigen p53 were isolated from a library constructed using 17S fraction of mRNA from the SV40 transformed murine fibroblasts (SVT2). These clones contain the whole coding region of p53 mRNA and the most of non-translated sequences. A plasmid containing 1.8 kb insert of p53 cDNA was constructed. The p53 specific insert in this plasmid was colinear with p53 mRNA, as revealed by S1 nuclease analysis. 5'-region of the p53 gene comprising non-translated and promoter areas was cloned from the mouse genomic library. A combined clone containing promoter and the whole region, corresponding to p53 mRNA has been constructed.
authors.forename: P M
authors.initials: PM
authors.lastname: Chumakov
authors.forename: V S
authors.initials: VS
authors.lastname: Iotsova
authors.forename: N N
authors.initials: NN
authors.lastname: Ninkina
authors.forename: V L
authors.initials: VL
authors.lastname: Bukhman
authors.forename: G P
authors.initials: GP
authors.lastname: Georgiev
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005347:Fibroblasts; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010750:Phosphoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D013539:Simian virus 40; D016159:Tumor Suppressor Protein p53
chemical_list: D009363:Neoplasm Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D004247:DNA; D004262:DNA Restriction Enzymes
vernacular_title: Vydelenie i kharakteristika klonov, komplementarnykh mRNK myshinogo kletochnogo opukholevogo antigena p53.