title: Potentiation of Alu I-induced chromosome aberrations by high salt concentrations in Chinese hamster ovary cells.
abstract: The restriction endonuclease Alu I induces chromosome-type aberrations in Chinese hamster ovary cells whose frequencies are considerably elevated in the presence of high concentrations of MgCl2, (NH4)2SO4, CaCl2 or NaCl. The most plausible explanation for these findings is that salt leads to partial dehistonization of the chromatin which makes more recognition sites available for Alu I.
authors.affiliation: Institut für Genetik, Freie Universität Berlin, F.R.G.
authors.forename: S
authors.initials: S
authors.lastname: Tuschy
authors.forename: G
authors.initials: G
authors.lastname: Obe
mesh_terms: D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000648:ultrastructure; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004262:DNA Restriction Enzymes / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D012492:Salts
chemical_list: D002843:Chromatin; D003470:Culture Media; D012492:Salts; D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific