Osteonectin cDNA sequence reveals potential binding regions for calcium and hydroxyapatite and shows homologies with both a basement membrane protein (SPARC) and a serine proteinase inhibitor (ovomucoid).
Osteonectin is a prominent noncollagenous protein of developing bone. A 2150-base-pair cDNA coding for osteonectin, isolated from a bovine bone cell lambda gt11 expression library, was sequenced and identified by comparison with protein sequence data. The nucleotide sequence predicts that osteonectin contains 304 amino acids, including a 17-residue signal peptide. Analysis of the deduced protein sequence suggests that the secreted protein contains at least four distinct structural domains. An acidic region at the amino terminus of the protein appears to be a potential hydroxyapatite-binding site. This is followed by a second domain, rich in cysteine, that shows sequence homology with cysteine-rich domains in turkey ovomucoid and other serine proteinase inhibitors. Two sequences homologous with central calcium-binding loops of "EF hands" and thus having potential to be high-affinity calcium-binding sites are located in two other domains within the carboxyl-terminal half of the protein. Finally, the osteonectin sequence shows near identity (greater than 90%) with another protein, SPARC (secreted protein, acidic and rich in cysteine), secreted by mouse parietal endoderm. These data suggest that osteonectin, a protein present in bone and other selected tissues, is a multifunctional protein.
Bolander
M E
ME
Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, Bethesda, MD 20892.
Young
M F
MF
Fisher
L W
LW
Yamada
Y
Y
Termine
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D004247:DNA / Q000032:analysis*; D017886:Durapatite; D004527:Egg Proteins / Q000378:metabolism*; D006882:Hydroxyapatites / Q000378:metabolism*; D008969:Molecular Sequence Data; D015676:Osteonectin; D010059:Ovomucin / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors
D002352:Carrier Proteins; D004527:Egg Proteins; D006882:Hydroxyapatites; D015676:Osteonectin; D015842:Serine Proteinase Inhibitors; D010059:Ovomucin; D004247:DNA; D017886:Durapatite; D002118:Calcium