Release of endogenous ATP during sympathetic nerve stimulation.
1 Vas deferens from guinea-pig was stimulated with a suction electrode and both contractions and release of endogenous ATP monitored 2 Release of ATP was tetrodotoxin-sensitive and increased when the number of stimuli was increased. 3 Release of ATP was not due to contraction of the muscle and persisted following block of contractions with prazosin and alpha, beta-methylene ATP. 4 These results indicate that stimulation of the sympathetic nerves in the vas deferens releases endogenous ATP presynaptically, supporting a cotransmitter function for ATP with noradrenaline.
Lew
M J
MJ
Baker Medical Research Institute, Prahran, Victoria, Australia.
White
T D
TD
D015761:4-Aminopyridine; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D014649:Vas Deferens / Q000378:metabolism
D000631:Aminopyridines; D010656:Phenylephrine; D013779:Tetrodotoxin; D000255:Adenosine Triphosphate; D015761:4-Aminopyridine; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D011224:Prazosin