Alpha-atrial natriuretic peptide binding sites in the rat choroid plexus are increased in the presence of hydrocephalus.
Specific alpha-rat atrial natriuretic peptide(1-28) [ANF-(99-126)] (rANP) binding sites in the choroid plexus of rats with kaolin-induced hydrocephalus were analyzed following incubation of related tissue sections with 125I-rANP, then using autoradiography and an image analysis coupled with computer-assisted micro-densitometry. The number of 125I-rANP binding sites in the choroid plexus of these rats was significantly higher, as compared to findings in the control rats, whereas no differences in the binding affinity were observed, 3 days and 3 weeks after the intracisternal injection of kaolin. The possibility that atrial natriuretic peptide may play a significant role in the function of cerebrospinal fluid production by interacting with specific, high affinity receptors in the rat choroid plexus has to be considered.
Tsutsumi
K
K
Department of Neurosurgery, Nagasaki University School of Medicine, Japan.
Niwa
M
M
Himeno
A
A
Kurihara
M
M
Kawano
T
T
Ibaragi
M
M
Ozaki
M
M
Mori
K
K
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001665:Binding Sites; D002831:Choroid Plexus / Q000378:metabolism* / Q000503:physiopathology; D003936:Diagnosis, Computer-Assisted; D006849:Hydrocephalus / Q000139:chemically induced / Q000378:metabolism*; D007616:Kaolin; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*
D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D007616:Kaolin; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor