Studies on benzodiazepine receptor subtypes in a model of chronic spontaneous petit mal-like seizures.
Among the large variety of epilepsy models, differences in binding parameters on benzodiazepine (BZD) receptors could be demonstrated in some of them. A new model of petit mal-like seizures occurring spontaneously has been described in a strain of Wistar rats. The purpose of this study was to investigate the binding parameters on 'central' type and 'peripheral' type BZD receptor sites in epileptic and non-epileptic animals of this strain. Thus, using [3H]flunitrazepam as ligand, no modification was observed for 'central' type BZD sites in cortex, cerebellum and hippocampus. Nevertheless, using [3H]Ro 5-4864 as ligand, an important increase (125-150%) in the affinity constant on 'peripheral' type BZD sites was observed in epileptic rats, namely a lower affinity for the ligand on this receptor. Finally, no change occurred in the ratio between 'central' and 'peripheral' BZD receptor site apparent numbers.
Hariton
C
C
INSERM U. 278, Faculté de Pharmacie, Marseille, France.
Ciesielski
L
L
Cano
J P
JP
Mandel
P
P
D000818:Animals; D001570:Benzodiazepinones / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002908:Chronic Disease; D004832:Epilepsy, Absence / Q000378:metabolism*; D005445:Flunitrazepam / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000145:classification* / Q000378:metabolism; D012016:Reference Values
D001570:Benzodiazepinones; D011963:Receptors, GABA-A; C028513:4'-chlorodiazepam; D005445:Flunitrazepam