title: A human tRNA(iMet) gene produces multiple transcripts.
abstract: A third nonallelic locus of the human methionyl-tRNA multigene family (tRNA(iMet-3) was isolated. This gene, unlike two other tRNA(iMet) loci, lacks a remarkable run of T and C residues which functions as a termination of transcription signal. Instead, three tandem termination signals, each containing no more than four thymidylate residues, function as relatively inefficient termination signals. As a result, polymerase readthrough generates at least three transcripts in vitro. The efficiency of apparent termination varies significantly at these sites. All resulting transcripts appear to be processed in vitro.
authors.affiliation: Department of Human Genetics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
authors.forename: C L
authors.initials: CL
authors.lastname: Vnencak-Jones
authors.forename: S Z
authors.initials: SZ
authors.lastname: Wahab
authors.forename: Z E
authors.initials: ZE
authors.lastname: Zehner
authors.forename: W M
authors.initials: WM
authors.lastname: Holmes
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D012323:RNA Processing, Post-Transcriptional; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012358:RNA, Transfer, Met / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012345:RNA, Transfer, Amino Acid-Specific; D012358:RNA, Transfer, Met; D004262:DNA Restriction Enzymes