title: Distribution of fucosubstance in kidney and related neoplasms. Absence of lectin-reactive alpha-fucose from the vasculature of bilateral Wilm's tumors.
abstract: Two alpha-fucose-binding lectins, Ulex europaeus agglutinin I (UEA I) and Lotus tetragonolobus agglutinin, were employed to compare and contrast the distribution of fucosubstance in normal human kidneys and a variety of renal tumors. The study employed a total of 31 kidneys surgically removed for the presence of a variety of tumors, including 11 unilateral Wilms' tumors, two cases of bilateral Wilms' tumors, 13 renal cell carcinomas, two congenital mesoblastic nephromas, one renal oncocytoma, one neuroblastoma metastatic to the kidney, and one clear cell sarcoma of the kidney. The results show that UEA I-reactive fucosubstance is detected in vascular endothelium of all kidneys and tumors, except bilateral Wilms' tumors. The presence of UEA I-reactive alpha-fucose in the vasculature of unilateral but not bilateral Wilms' tumors defines a unique histochemical distinction between the two groups of tumors. Conceivably, this property might be exploited as a screening procedure for the more aggressive bilateral neoplasms. Other findings detail histochemical differences between UEA I and L tetragonolobus agglutinin, as evidenced by the ability of one lectin to stain a particular cell type that is not reactive with the other lectin.
authors.affiliation: Department of Pathology, Medical University of South Carolina, Charleston 29425.
authors.forename: R A
authors.initials: RA
authors.lastname: Hennigar
authors.forename: D A
authors.initials: DA
authors.lastname: Sens
authors.forename: H B
authors.initials: HB
authors.lastname: Othersen
authors.forename: A J
authors.initials: AJ
authors.lastname: Garvin
mesh_terms: D001808:Blood Vessels / Q000201:enzymology / Q000378:metabolism*; D002648:Child; D005643:Fucose / Q000378:metabolism*; D006735:Horseradish Peroxidase; D006801:Humans; D007223:Infant; D007668:Kidney / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000098:blood supply / Q000201:enzymology / Q000378:metabolism*; D037102:Lectins; D008297:Male; D037121:Plant Lectins; D014018:Tissue Distribution; D009396:Wilms Tumor / Q000098:blood supply / Q000201:enzymology*
chemical_list: D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; C027112:fucose-binding lectin; D005643:Fucose; D006735:Horseradish Peroxidase