Catecholamine stimulation of cortisol secretion by 3-day primary cultures of purified zona fasciculata/reticularis cells isolated from bovine adrenal cortex.
Primary cultures of zona fasciculata/reticularis cells derived from bovine adrenal cortex secreted cortisol and corticosterone in response to isoprenaline, noradrenaline and adrenaline on the third day of culture. The potency order was isoprenaline greater than noradrenaline, adrenaline with an ED50 for all three agonists within the range 1-5 x 10(-8) M. A dose-dependent increase in medium content of cyclic AMP was also observed. Secretion of cortisol in response to these catecholamines was specifically blocked by propranolol but unaffected by phentolamine. The beta-agonist effect on cortisol secretion was specifically and progressively reduced, in a time- and dose-dependent manner, by pre-incubation of the cells with adrenaline.
Walker
S W
SW
Department of Clinical Chemistry, Royal Infirmary of Edinburgh, U.K.
Lightly
E R
ER
Milner
S W
SW
Williams
B C
BC
D000302:Adrenal Cortex / Q000166:cytology* / Q000187:drug effects; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology*; D002417:Cattle; D002469:Cell Separation; D002478:Cells, Cultured; D003345:Corticosterone / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D006854:Hydrocortisone / Q000378:metabolism*; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011863:Radioimmunoassay / Q000379:methods; D013997:Time Factors
D002395:Catecholamines; D000324:Adrenocorticotropic Hormone; D011433:Propranolol; D000242:Cyclic AMP; D003345:Corticosterone; D006854:Hydrocortisone; D004837:Epinephrine; D010646:Phentolamine