title: Isolation, amino acid sequence and action of guinea-pig ACTH on aldosterone production by glomerulosa cells.
abstract: Though minor sequence differences between-species have been reported for adrenocorticotrophin, ACTH(1-39), the steroidogenic moiety ACTH(1-24) has appeared invariant in mammals. We here report the isolation, purification and amino acid sequencing of guinea-pig (GP) ACTH in which Pro24 is replaced by Ala24, and the demonstration that GP-ACTH stimulates aldosterone production to maximal levels well above those seen with human ACTH(1-39) or Synacthen, ACTH(1-24) amide, the synthetic ACTH fragment widely used for diagnostic and therapeutic purposes.
authors.affiliation: Medical Research Centre, Prince Henry's Hospital, Melbourne, Australia.
authors.forename: A I
authors.initials: AI
authors.lastname: Smith
authors.forename: C A
authors.initials: CA
authors.lastname: Wallace
authors.forename: R L
authors.initials: RL
authors.lastname: Moritz
authors.forename: R J
authors.initials: RJ
authors.lastname: Simpson
authors.forename: L B
authors.initials: LB
authors.lastname: Schmauk-White
authors.forename: E A
authors.initials: EA
authors.lastname: Woodcock
authors.forename: J W
authors.initials: JW
authors.lastname: Funder
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000302:isolation & purification* / Q000494:pharmacology; D000450:Aldosterone / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D000818:Animals; D006168:Guinea Pigs; D008969:Molecular Sequence Data
chemical_list: D000450:Aldosterone; D000324:Adrenocorticotropic Hormone