Enrichment of the spindle fiber attachments from mouse liver cell nuclei.
Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouse livers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and high speed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured by radioimmunoassay. The basic method offers opportunities for further increases of yield and for the enrichment of SFA of other vertebrates.
Shi
L J
LJ
Shanghai Institute of Cell Biology, Academia Sinica.
Ni
Z M
ZM
Lu
S F
SF
Wang
G
G
Qi
H L
HL
Su
Q X
QX
D000818:Animals; D002467:Cell Nucleus / Q000276:immunology; D015342:DNA Probes; D007074:Immunoglobulin G; D008099:Liver / Q000648:ultrastructure; D051379:Mice; D012595:Scleroderma, Systemic / Q000235:genetics*; D008941:Spindle Apparatus; D014162:Transfection
D015342:DNA Probes; D007074:Immunoglobulin G