Identification of the lesion in the stimulatory GTP-binding protein of the uncoupled S49 lymphoma.
The stimulatory GTP-binding protein (Gs) of the uncoupled mutant of S49 lymphoma cells is deficient in its ability to transduce hormonal signals from ligand-bound beta-adrenergic receptors to the catalytic component of adenylate cyclase. In order to define the genetic defect in the Gs of uncoupled S49 cells, a complementary DNA clone encoding the alpha-subunit of Gs was analyzed and the deduced primary structure of the defective subunit compared to that of the wild-type subunit. A single nucleotide transversion was found that coded for a proline rather than an arginine at residue 389. The results indicate a domain of the alpha-subunit of Gs that specifically interacts with hormone receptors.
Rall
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Merrell Dow Research Institute, Strasbourg, France.
Harris
B A
BA
D000262:Adenylyl Cyclases / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D008223:Lymphoma / Q000503:physiopathology*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011943:Receptors, Adrenergic, beta; D004247:DNA; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases