Expression of human c-raf-1 oncogene proteins in E. coli.
Full length and truncated versions of the human c-raf-1 cDNA were cloned into the inducible E. coli expression vector pJL6. C-raf proteins of 73 kD, 57 kD and 39 kD were produced upon induction. p73 differs from normal p73 c-raf by deletion of the two first N-terminal amino acids and their replacement by 16 amino acids encoded by the vector. The p57 and p39 represent N-terminal deletions which leave the transforming protein kinase domain intact. These proteins could be readily purified from E. coli lysates by immunoprecipitation with raf-specific antisera.
Kolch
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Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, Maryland 21701-1013.
Bonner
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Rapp
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D000596:Amino Acids / Q000032:analysis; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D008970:Molecular Weight; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf
D000596:Amino Acids; D011518:Proto-Oncogene Proteins; D019908:Proto-Oncogene Proteins c-raf; D004262:DNA Restriction Enzymes