Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase.
The gene gyrA of Escherichia coli, which encodes the A subunit of DNA gyrase (topoisomerase II), has been cloned and a region of approximately 3300 base-pairs sequenced. An open reading frame of 2625 nucleotides coding for a protein of 97,000 Mr is located. The peptide weight of the subunit predicted from this open reading frame is in close agreement with previously published estimates of that of the A subunit. There is a "TATAAT" promoter motif located 44 bases upstream from the first "ATG" of the open reading frame. The amino acid sequence derived from the nucleotide sequence is about 50% homologous with that derived from the Bacillus subtilis gyrA gene sequence, with several regions showing greater than 90% homology.
Swanberg
S L
SL
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Wang
J C
JC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008969:Molecular Sequence Data
D003062:Codon; D046911:Macromolecular Substances; D004250:DNA Topoisomerases, Type II