Site-dependent effects of ions on mu-, delta- and kappa-opioid binding in suspensions of guinea-pig brain membranes.
NaCl, LiCl, NH4Cl and KCl inhibit the equilibrium binding of peptide agonists at each of the mu-, delta- and kappa-sites; the orders of potencies and the slopes of the dose-response curves are site-dependent. In contrast, the monovalent salt choline chloride inhibits mu- and kappa-binding but has little effect on delta-binding. The profiles of activity of MnCl2, CaCl2 and MgCl2 are also site-dependent but differ markedly from those of the monovalent salts.
Robson
L E
LE
Unit for Research on Addictive Drugs, University of Aberdeen, Marischal College, U.K.
Paterson
S J
SJ
Kosterlitz
H W
HW
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D006168:Guinea Pigs; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D011189:Potassium Chloride / Q000494:pharmacology; D011957:Receptors, Opioid / Q000187:drug effects / Q000378:metabolism*; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D012965:Sodium Chloride / Q000494:pharmacology
D002712:Chlorides; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D000643:Ammonium Chloride; D012965:Sodium Chloride; D011189:Potassium Chloride; D008094:Lithium; D018021:Lithium Chloride