title: Conservation of a gene cluster including glycoprotein B in bovine herpesvirus type 2 (BHV-2) and herpes simplex virus type 1 (HSV-1).
abstract: A library of subgenomic fragments of bovine herpesvirus type 2 (BHV-2) DNA was constructed in the expression cloning vector lambda gt11 and screened with monoclonal antibodies to the glycoprotein gb BHV-2, which is homologous to glycoprotein gB (gB-1) of herpes simplex virus type 1 (HSV-1). Lambda gt11 clones containing gB BHV-2-specific sequences were used to identify lambda EMBL3 vectors with DNA inserts which contained the complete gB BHV-2 gene. Nucleotide sequencing revealed that the gB BHV-2 gene is highly conserved compared to gB-1. The amino acid sequences and the predicted secondary structures of both glycoproteins are very similar. Two further open reading frames (ORF) in close vicinity to the gene encoding gB BHV-2 showed considerable homology to HSV-1 genes. They code for the major DNA-binding protein (dbp) of BHV-2 and a putative 72-kDa polypeptide. The gene of the latter protein corresponding to ICP18.5 of HSV-1 is interspersed between the ORFs of gB BHV-2 and the dbp of BHV-2. All three genes map in the unique long region of the genome. Their homology and the colinear arrangement compared to HSV-1 indicate a close relationship between the two viruses.
authors.affiliation: Institut für Virologie der Freien Universität, Berlin, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Hammerschmidt
authors.forename: F
authors.initials: F
authors.lastname: Conraths
authors.forename: J
authors.initials: J
authors.lastname: Mankertz
authors.forename: G
authors.initials: G
authors.lastname: Pauli
authors.forename: H
authors.initials: H
authors.lastname: Ludwig
authors.forename: H J
authors.initials: HJ
authors.lastname: Buhk
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D001911:Herpesvirus 2, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D004247:DNA