Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.
A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.
Datta
A R
AR
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
Wentz
B A
BA
Hill
W E
WE
D001345:Autoradiography; D002499:Centrifugation, Density Gradient; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification* / Q000528:radiation effects; D008872:Microwaves; D009693:Nucleic Acid Hybridization
D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases