Intracellular location of T200 and Mo1 glycoproteins in human neutrophils.
Mo1 (CD11b), a glycoprotein heterodimer that is involved in cellular adhesion processes and functions as the C3bi receptor of human myeloid cells, and T200 (CD45), a panleukocyte glycoprotein family whose function is still not well understood, increased their expression in the plasma membrane of human neutrophils after exposure to various stimuli which induce degranulation, such as formylmethionylleucylphenylalanine or calcium ionophore A23187. This increment in the expression of both molecules shows a good correlation with the release to the extracellular environment of gelatinase, a marker for an intracellular organelle named "tertiary granule" (Mollinedo, F., and Schneider, D. L. (1984) J. Biol. Chem. 259, 7143-7150). Flow cytometry studies indicate that at least 50% of the total Mo1 and T200 molecules are located in intracellular organelles. Furthermore, the subcellular distribution of Mo1 and T200 glycoproteins in resting human neutrophils was investigated by immunoprecipitation of the radiolabeled membrane proteins obtained from the distinct subcellular fractions. Both Mo1 and T200 were mainly localized in tertiary or specific intracellular granules, which were resolved from the azurophilic granules as well as from the cell membrane fraction. These findings suggest that the mobilization of intracellular Mo1 and T200 to the plasma membrane may regulate early events occurring upon neutrophil activation.
Lacal
P
P
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Pulido
R
R
Sánchez-Madrid
F
F
Mollinedo
F
F
D000943:Antigens, Differentiation / Q000032:analysis*; D000954:Antigens, Surface; D001798:Blood Proteins; D000001:Calcimycin / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000378:metabolism; D005434:Flow Cytometry; D018093:Gelatinases; D005966:Glucuronidase / Q000097:blood; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D007163:Immunosorbent Techniques; D017493:Leukocyte Common Antigens; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins / Q000097:blood*; D009113:Muramidase / Q000097:blood; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D009940:Organoids / Q000378:metabolism; D010434:Pepsin A / Q000097:blood; D013696:Temperature
D000943:Antigens, Differentiation; D000954:Antigens, Surface; D001798:Blood Proteins; D006649:Histocompatibility Antigens; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D000001:Calcimycin; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D017493:Leukocyte Common Antigens; D009113:Muramidase; D005966:Glucuronidase; D010434:Pepsin A; D018093:Gelatinases