title: The ATP,Mg-dependent protein phosphatase: regulation by casein kinase-1.
abstract: The free modulator subunit of the ATP,Mg-dependent phosphatase is phosphorylated up to 1 mol per mol by casein kinase-1, up to 1.85 mol per mol after dephosphorylation by the PCSH1 phosphatase, but 10-fold less when purified in the presence of NaF, suggesting an in vivo phosphorylation of the casein kinase-1 sites. Peptide mapping of 32P-modulator labeled by casein kinase-1 or -2 shows a different phosphorylation pattern. Phosphorylation of the inactive phosphatase by casein kinase-1 prevents the subsequent kinase FA-mediated activation, while it does not impair the activated phosphatase.
authors.affiliation: Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit te Leuven, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Agostinis
authors.forename: J R
authors.initials: JR
authors.lastname: Vandenheede
authors.forename: J
authors.initials: J
authors.lastname: Goris
authors.forename: F
authors.initials: F
authors.lastname: Meggio
authors.forename: L A
authors.initials: LA
authors.lastname: Pinna
authors.forename: W
authors.initials: W
authors.lastname: Merlevede
mesh_terms: D047388:Casein Kinases; D004789:Enzyme Activation; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000502:physiology*
chemical_list: D010750:Phosphoproteins; D011494:Protein Kinases; D047388:Casein Kinases; D010749:Phosphoprotein Phosphatases