Cytochalasin stimulates phosphoinositide metabolism in murine B lymphocytes.
The ability of cytochalasin to alter phosphoinositide metabolism was evaluated in naive, murine splenic B lymphocytes. The generation of total inositol phosphates was stimulated by treatment with cytochalasin D, as was the generation of inositol trisphosphate. The cytochalasin-induced increase in inositol phosphates appeared to depend on interaction with actin, and it was inhibited by phorbol esters. Inositol phosphate production was not stimulated in splenic T lymphocytes. The results suggest that the previously observed increase in intracellular calcium produced by cytochalasin is part of a more generalized signaling event that includes phosphoinositide turnover, and, further, raise the possibility of a functional association between actin and phospholipase C.
van Haelst
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Evans Memorial Department of Clinical Research, Boston University Medical Center, MA 02118.
Rothstein
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D000199:Actins / Q000378:metabolism; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D015638:Cytochalasin D; D003572:Cytochalasins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D026121:Indole Alkaloids; D007211:Indoles / Q000494:pharmacology; D051379:Mice; D008805:Mice, Inbred A; D010703:Phorbol Esters / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
D000199:Actins; D003572:Cytochalasins; D026121:Indole Alkaloids; D007211:Indoles; D010703:Phorbol Esters; D010716:Phosphatidylinositols; D015638:Cytochalasin D; C019290:chaetoglobosins