Adrenocortical suppression by topically applied corticosteroids in healthy dogs.
Three corticosteroid products (triamcinolone acetonide, fluocinonide, betamethasone valerate) and a control product composed of water, petrolatum, mineral oil, cetyl alcohol, steryl alcohol, sodium lauryl sulfate, cholesterol, and methylparaben each were applied topically to healthy dogs (5 dogs/product) once daily for 5 consecutive days. Plasma concentrations of immunoreactive adrenocorticotropic hormone (iACTH) and cortisol were determined before 1 microgram of ACTH/kg of body weight was given intravenously (pre-ACTH values) and cortisol was again measured 60 minutes after ACTH was given (post-ACTH values). Cortisol and iACTH concentrations were determined in each dog before, during, and after administration of the corticosteroid products. All 3 corticosteroids caused prompt and sustained pituitary-adrenocortical suppression. Compared with control applications, the application of corticosteroids resulted in significant reduction of plasma cortisol and iACTH concentrations by day 2 of treatment, and the lower concentrations continued to day 5. One week after the last application of the corticosteroids, plasma iACTH concentrations in the corticosteroid-treated dogs had returned to the range of values for the control dogs; however, pre- and post-ACTH cortisol concentrations remained suppressed in all corticosteroid-treated dogs. Two weeks after the last treatment, the pre-ACTH plasma cortisol concentrations of corticosteroid-treated dogs returned to those of the control dogs, but the post-ACTH plasma cortisol concentrations remained suppressed. By 3 weeks after the last treatment, post-ACTH plasma cortisol concentrations of dogs treated with triamcinolone acetonide had returned to the range of values for the control dogs, but remained suppressed in the other 2 groups of dogs. All indices of pituitary-adrenocortical activity were within the control range by 4 weeks after the last treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
Zenoble
R D
RD
Department of Small Animal Surgery, College of Veterinary Medicine, Auburn University, AL 36849.
Kemppainen
R J
RJ
D000287:Administration, Topical; D000302:Adrenal Cortex / Q000187:drug effects* / Q000502:physiology; D000304:Adrenal Cortex Function Tests / Q000662:veterinary; D000305:Adrenal Cortex Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D001624:Betamethasone Valerate / Q000008:administration & dosage / Q000494:pharmacology; D004285:Dogs / Q000502:physiology*; D005260:Female; D005447:Fluocinonide / Q000008:administration & dosage / Q000494:pharmacology; D006854:Hydrocortisone / Q000097:blood; D008297:Male; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000502:physiology; D011897:Random Allocation; D012869:Skin Absorption; D014222:Triamcinolone Acetonide / Q000008:administration & dosage / Q000494:pharmacology
D000305:Adrenal Cortex Hormones; D005447:Fluocinonide; D000324:Adrenocorticotropic Hormone; D001624:Betamethasone Valerate; D014222:Triamcinolone Acetonide; D006854:Hydrocortisone