title: Characterization of the mouse met proto-oncogene.
abstract: The DNA sequence of cDNA clones prepared from transcripts of the mouse met proto-oncogene reveals that the mouse met gene encodes a 1380 amino acid protein with the characteristics of a growth factor receptor. This protein can be divided into several putative domains, including an intracellular protein tyrosine kinase domain, a transmembrane domain and a 929 amino acid extracellular domain, possessing a potential proteolytic cleavage site with the sequence Lys-Arg-Arg-Lys-Arg-Ser. To gain additional insights into the function of the met protein we have examined the level of met transcripts in tissues of the late-gestation mouse conceptus. Transcription of met was observed in most of the tissues analysed, but the highest levels of met mRNA were detected in the yolk sac, amnion and kidney; no transcripts were detectable in the calvaria. Chromosomal localization using a series of mouse-hamster hybrid cell lines has demonstrated that met is located on mouse chromosome 6.
authors.affiliation: Chemical Carcinogenesis Section, Chester Beatty Laboratories, London, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Chan
authors.forename: H W
authors.initials: HW
authors.lastname: King
authors.forename: E A
authors.initials: EA
authors.lastname: Deakin
authors.forename: P R
authors.initials: PR
authors.lastname: Tempest
authors.forename: J
authors.initials: J
authors.lastname: Hilkens
authors.forename: V
authors.initials: V
authors.lastname: Kroezen
authors.forename: D R
authors.initials: DR
authors.lastname: Edwards
authors.forename: A J
authors.initials: AJ
authors.lastname: Wills
authors.forename: P
authors.initials: P
authors.lastname: Brookes
authors.forename: C S
authors.initials: CS
authors.lastname: Cooper
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006133:Growth Substances; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D011956:Receptors, Cell Surface / Q000235:genetics*
chemical_list: D006133:Growth Substances; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface