title: Adaptation in the motility response to cAMP in Dictyostelium discoideum.
abstract: When developing amebae of Dictyostelium discoideum are treated with constant concentrations of cAMP above 10(-8)M, the average rate of motility is depressed, with maximum inhibition at roughly 10(-6)M. It is demonstrated that shifting the concentration of cAMP from 0 M to concentrations ranging from 10(-8) to 10(-6)M in a perfusion chamber results in the immediate inhibition of motility. After shifting from 0 M to 10(-8) or 10(-7)M, the rate of cell motility remains low, then rebounds to a higher level, exhibiting a standard adaptation response. No adaptation is exhibited after a shift from 0 M to 10(-6)M, a concentration resulting in maximum inhibition. It is demonstrated that the level of inhibition and the extent of the adaptation period are dependent upon the concentration of cAMP after the shift, and that submaximal inhibition is additive. The characteristics of adaptation in this motility response are very similar to the characteristics of adaptation for the relay system and phosphorylation of the putative cAMP receptor.
authors.affiliation: Department of Biology, University of Iowa, Iowa City 52242.
authors.forename: B
authors.initials: B
authors.lastname: Varnum-Finney
authors.forename: N A
authors.initials: NA
authors.lastname: Schroeder
authors.forename: D R
authors.initials: DR
authors.lastname: Soll
mesh_terms: D002633:Chemotaxis; D000242:Cyclic AMP / Q000494:pharmacology*; D004023:Dictyostelium / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D007700:Kinetics; D013997:Time Factors
chemical_list: D000242:Cyclic AMP