title: Poliovirus type 1/type 3 antigenic hybrid virus constructed in vitro elicits type 1 and type 3 neutralizing antibodies in rabbits and monkeys.
abstract: Poliovirus exists as three stable serotypes (PV-1, PV-2, and PV-3). These viruses display three antigenic sites each, designated N-AgI, N-AgII, and N-AgIII. When mice are immunized with poliovirus, N-AgI is the major neutralization antigenic site for PV-3, whereas N-AgII and N-AgIII are immunodominant over N-AgI for PV-1. To study the relationship between structure and antigenicity, a hybrid virus was constructed in which N-AgI of PV-1 was replaced by N-AgI of PV-3. PV-3- and PV-1-specific antisera, including those elicited by PV-3 in primates, neutralized the hybrid virus. Injection of the hybrid virus into rabbits or into primates resulted in the production of antisera that neutralized both PV-1 and PV-3. The data show that sequence replacement at N-AgI of poliovirus is compatible with viral proliferation, an observation useful for the development of multivalent picornavirus vaccines.
authors.affiliation: Department of Microbiology, State University of New York, Stony Brook 11794-8621.
authors.forename: M G
authors.initials: MG
authors.lastname: Murray
authors.forename: R J
authors.initials: RJ
authors.lastname: Kuhn
authors.forename: M
authors.initials: M
authors.lastname: Arita
authors.forename: N
authors.initials: N
authors.lastname: Kawamura
authors.forename: A
authors.initials: A
authors.lastname: Nomoto
authors.forename: E
authors.initials: E
authors.lastname: Wimmer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D001483:Base Sequence; D000882:Haplorhini; D009154:Mutation; D009841:Oligonucleotides / Q000032:analysis; D017955:Poliovirus / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated; D011817:Rabbits
chemical_list: D000914:Antibodies, Viral; D009841:Oligonucleotides; D011054:Poliovirus Vaccine, Inactivated