Nucleotide sequence of integrated hepatitis B virus DNA and human flanking regions in the genome of the PLC/PRF/5 cell line.
Three fragments of cellular DNA containing integrated hepatitis B virus (HBV) sequences have been cloned from the genomic library of a PLC/PRF/5 cell line. The complete nucleotide sequences of the HBV DNA inserts have been determined, including the S gene and the cellular flanking DNA. Viral sequences were found to be fragmented and rearranged. The nucleotide sequences of the HBV-HBV and HBV-human DNA junctions in two of the clones were precisely the same as described by others for the analogous HBV-DNA inserts, providing direct evidence for the stability of HBV-DNA integration pattern and sequence in the genome of the PLC/PRF/5 line. Two clones contain the HBV surface antigen gene which is well conserved. According to the amino acid sequence it could be related to the adw subtype. HBV DNA and contiguous human sequences in HC217 clone are flanked by the cellular perfect inverted repeat of at least 3.5 kb. Similar sequences have been found in the genome of the original PLC/PRF/5 cell line and human placental DNA.
Rivkina
M B
MB
August Kirchenstein Institute of Microbiology, Latvian SSR Academy of Sciences.
Lunin
V G
VG
Mahov
A M
AM
Tikchonenko
T I
TI
Kukain
R A
RA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D008969:Molecular Sequence Data
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D004262:DNA Restriction Enzymes