[Presence of antibodies specifically reacting with structural proteins of the mammary cancer virus among antibodies found in circulating immune complexes in patients with breast cancer].
Circulating immune complexes were precipitated from breast cancer patients' sera using 2.5% polyethylene glycol. CIC isolated from 70 ml of sera from 15 patients were dissociated and immunoglobulin-containing fraction was prepared by chromatography on Sephadex G-200 column. The fraction contained IgG specific for MuMTV structural proteins, as revealed by ELISA. CIC preparations from 22 sera of breast cancer patients were digested with pepsin; Fab' fragment preparations were also analysed by ELISA, only one of them was MuMTV-specific. IgG and Fab' fragments isolated from CIC reacted specifically with MuMTV proteins, the reaction was not blocked by virus-free murine milk or other retroviruses (Ra-MuLV and MPMV).
Litvinov
S V
SV
Malivanova
T F
TF
Chuev
Iu V
IuV
Kriukova
I N
IN
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000276:immunology*; D005260:Female; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D051379:Mice
D000912:Antibodies, Neoplasm; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral
Prisutstvie antitel, spetsificheski reagiruiushchikh so strukturnymi belkami virusa raka molochnoĭ zhelezy (MMTV), sredi antitel, vkhodiashchikh v sostav tsirkuliruiushchikh immunnykh kompleksov u bol'nykh rakom molochnoĭ zhelezy.