[Effect of 5'-deoxy-5'-S-isobutyl adenosine (SIBA) on the synthesis of polyoma virus constituents in infected mouse cells].
At 100 microM 5'-S-isobutyladenosine (SIBA) inhibits polyoma virus production in infected mouse embryo fibroblasts and in mouse kidney cells, as measured by plaque formation and by haemagglutination assays. SIBA has no significant effect on the synthesis of T and V antigens as well as on viral DNA synthesized in infected cells. Analysis of virus production on CsCl gradients on CsCl gradients showed that in the presence of SIBA reduced amount of heavy viral particles is produced and that part of these particles are pseudovirions containing low density DNA instead of supercoiled viral DNA.
Raies
A
A
Ecole supérieure des Industries alimentaires, Tunisie.
Lawrence
F
F
Robert-Géro
M
M
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004261:DNA Replication; D003839:Deoxyadenosines / Q000031:analogs & derivatives* / Q000494:pharmacology; D004622:Embryo, Mammalian; D005347:Fibroblasts; D006384:Hemagglutination; D007668:Kidney; D051379:Mice; D011120:Polyomavirus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D013872:Thionucleosides / Q000494:pharmacology*
D000952:Antigens, Polyomavirus Transforming; D000998:Antiviral Agents; D003839:Deoxyadenosines; D013872:Thionucleosides; C013919:5'-deoxy-5'-S-isobutylthioadenosine
Effet de la 5'-désoxy-5'-S-isobutyl adénosine (SIBA) sur la synthèse des constituants du virus polyome dans des cellules de souris infectées.