Optimum pH levels for eluting enteroviruses from sludge solids with beef extract.
This study demonstrates that elution of enteroviruses from a mixture of primary- and activated-sludge solids with beef extract at pH 9.2 +/- 0.2 may be less efficient than elution with beef extract at pH 7.2 +/- 0.2 and that elution of enteroviruses from extended-aeration-sludge solids with beef extract is at best no more efficient at pH 9.2 +/- 0.2 than at pH 7.2 +/- 0.2. Thus, the common practice of adjusting the pH of beef extract used for eluting enteroviruses from the natural neutral level of the elutant to alkaline levels is unnecessary and probably undesirable.
Berg
G
G
Department of Civil and Environmental Engineering, University of Cincinnati, Ohio 45221.
Sullivan
G
G
D000818:Animals; D002417:Cattle; D004770:Enterovirus / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D012722:Sewage; D014020:Tissue Extracts
D012722:Sewage; D014020:Tissue Extracts