Cell-mediated immunity induced in mice after vaccination with a protease activation mutant, TR-2, of Sendai virus.
Our previous study has shown that, although a trypsin-resistant mutant of Sendai virus, TR-2, replicates only in a single cycle in mouse lung with a negligible lesion, the animal acquires a strong immunity against lethal infection with wild-type Sendai virus, suggesting that TR-2 could be used as a new type of live vaccine (M. Tashiro and M. Homma, J. Virol. 53:228-234, 1985). In the present study, we investigated the immunological response elicited in TR-2-infected mice, particularly with respect to cell-mediated immunity. Analyses of cytotoxic activities of spleen cells with 51Cr release assays revealed that Sendai virus-specific T lymphocytes (CTL), in addition to natural killer activity and antiviral antibodies, were induced in DBA/2 and C3H/He mice infected intranasally with TR-2. Proteolytic activation of the fusion glycoprotein F was required for the primary induction of CTL, though not necessarily for stimulation of natural killer and antibody responses. Memory of the CTL induced by TR-2 was long-lasting and was recalled in vivo immediately after challenge with wild-type Sendai virus. In contrast to TR-2, immunization with inactive split vaccine failed to induce the CTL response, but it elicited a high titer of serum antibody and a low level of natural killer activity.
Tashiro
M
M
Department of Bacteriology, Yamagata University School of Medicine, Japan.
Fujii
Y
Y
Nakamura
K
K
Homma
M
M
D000818:Animals; D003673:Defective Viruses / Q000235:genetics / Q000276:immunology; D007111:Immunity, Cellular; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D010222:Parainfluenza Virus 1, Human / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology*; D014779:Virus Replication
D014765:Viral Vaccines