Primary structure of soybean lipoxygenase L-2.
The nucleotide sequence of soybean lipoxygenase-2 cDNA has been determined, and the complete amino acid sequence of the enzyme has been deduced. Limited direct amino acid sequence data for lipoxygenase-2 protein support this assignment and exclude mRNA representing lipoxygenase-1 and -3. Lipoxygenase 2 has a molecular weight of 97,036 and contains 865 amino acid residues, in contrast to the isozymes, lipoxygenase-1 and -3, which are known to contain 838 and 859 amino acid residues, respectively. Despite significant differences in behavior between these three isozymes, the amino acid sequences of lipoxygenase-1 and -3 are 81 and 74% identical to lipoxygenase-2, respectively. A region of 40 amino acid residues containing a cluster of six histidines and two tyrosines, which is highly conserved in all three isozymes, is discussed as a possible iron-binding region.
Shibata
D
D
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Steczko
J
J
Dixon
J E
JE
Andrews
P C
PC
Hermodson
M
M
Axelrod
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005796:Genes; D008084:Lipoxygenase / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000201:enzymology / Q000235:genetics
D003062:Codon; D004247:DNA; D008084:Lipoxygenase; D004262:DNA Restriction Enzymes