title: Use of a simple method for the Epstein-Barr virus transformation of lymphocytes from members of large families of Réunion Island.
abstract: A simple method for the preparation of lymphoblastoid cell lines from small amounts (100 microliter) of frozen whole blood is described. A success score greater than 90% was obtained for EBV transformations using blood samples which had been collected several months before the infection. Due to the simplicity of the technique, up to 80 samples could be processed per day. This technique was used to prepared 242 permanent cell lines from 13 large families from Réunion Island showing blood group H deficiency. These cell lines are now available for genetic studies.
authors.affiliation: Institut d'Immunobiologie, CNRS ER-281, Hôpital Broussais, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Ventura
authors.forename: A
authors.initials: A
authors.lastname: Gibaud
authors.forename: J
authors.initials: J
authors.lastname: Le Pendu
authors.forename: D
authors.initials: D
authors.lastname: Hillaire
authors.forename: G
authors.initials: G
authors.lastname: Gérard
authors.forename: D
authors.initials: D
authors.lastname: Vitrac
authors.forename: R
authors.initials: R
authors.lastname: Oriol
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics; D002460:Cell Line; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D010375:Pedigree
chemical_list: D000017:ABO Blood-Group System