Chemical-enzymatic synthesis, cloning and expression of a synthetic gene coding for an env protein fragment of the human T-cell leukemia virus type 1.
A synthetic gene for a 88 amino acid long env protein fragment of the human T-cell leukemia virus type 1 (HTLV1) has been assembled by ligation of 35 oligodesoxyribonucleotides, which were chemically synthesized by the phosphotriester segmental support method. After cloning into the pEX vector this HTLV1 env-protein fragment was expressed in E. coli.
Rosenthal
A
A
Akademie der Wissenschaften der DDR, Zentralinstitut für Molekularbiologie, Berlin-Buch.
Ulrich
R
R
Billwitz
H
H
Frank
R
R
Blöcker
H
H
D003001:Cloning, Molecular; D004279:DNA, Viral / Q000138:chemical synthesis*; D017977:Deltaretrovirus / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005813:Genes, Synthetic; D005814:Genes, Viral; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics*
D004279:DNA, Viral; D014759:Viral Envelope Proteins