title: Plurality of genital human papillomaviruses: characterization of two new types with distinct biological properties.
abstract: The genomes of two new genital human papillomavirus (HPV) types, tentatively named HPVs 39 and 42, have been cloned from biopsy specimens of penile Bowenoid papules and vulvar papillomas, respectively. Blot hybridization experiments, performed under stringent conditions (Tm -10 degrees), have revealed no cross-hybridization between the DNAs of HPVs 39 and 42, and between these DNAs and those of other genital and cutaneous HPVs. A significant cross-hybridization has been observed between the DNA of HPV42 and that of HPV32, the latter being associated with oral focal epithelial hyperplasia. The fraction of HPV32 and HPV42 hybrid molecules resistant to nuclease S1 treatment after hybridization in liquid phase at saturation has been evaluated to 20%, supporting the view that these HPVs constitute distinct types. In addition to HPV42 DNA, a 6.8-kb BamHI fragment, cross-hybridizing with HPV39 DNA, has been cloned from the vulvar papilloma DNA preparation. The cross-hybridization has been evaluated to 16%, pointing to the existence of an additional HPV39-related type. Electron microscope analysis of heteroduplex molecules formed between HPV32 and HPV42 DNAs showed paired regions over about 60 and 87% of their genome lenghts under stringent (Tm -18 degrees) and nonstringent (Tm -42 degrees) conditions, respectively. The 6.8-kb HPV DNA and HPV39 DNA formed paired regions over about 63 and 95% of the 6.8-kb fragment length at Tm -18 degrees and Tm -26 degrees, respectively. These data point to greater DNA sequence homologies than anticipated from the percentages of nuclease S1 resistance. Heteroduplex mapping has allowed the alignment of the physical maps of HPV39 and 42 DNAs and of the 6.8-kb HPV DNA with the map of the open reading frames of the HPV16 genome. So far, HPV42 has been detected only in benign genital lesions showing usually no cell atypia. HPV39 has been detected in a few cases of intraepithelial neoplasias and invasive carcinomas of the uterine cervix. The viral DNA sequences have been found integrated into the cell genome in all four HPV39-associated cervical cancers of our series. It seems most likely that HPV42 belongs to the low-risk group of genital HPVs, while HPV39 represents a potentially oncogenic genital HPV type.
authors.affiliation: Unité de l'Institut National de la Santé et de la Recherche Médicale 190, Institut Pasteur, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Beaudenon
authors.forename: D
authors.initials: D
authors.lastname: Kremsdorf
authors.forename: S
authors.initials: S
authors.lastname: Obalek
authors.forename: S
authors.initials: S
authors.lastname: Jablonska
authors.forename: G
authors.initials: G
authors.lastname: Pehau-Arnaudet
authors.forename: O
authors.initials: O
authors.lastname: Croissant
authors.forename: G
authors.initials: G
authors.lastname: Orth
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000382:microbiology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D010412:Penile Neoplasms / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D002583:Uterine Cervical Neoplasms / Q000382:microbiology; D014846:Vulvar Neoplasms / Q000382:microbiology*
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes