title: Action of food restriction on age-related changes in adipocyte lipolysis.
abstract: The effects of aging and of food restriction at different times during life on rat adipocyte responses to glucagon and epinephrine were explored by studying hormone-stimulated lipolysis, hormone binding, and phosphodiesterase activity. The times of food restriction were: (a) from 6 weeks of age, (b) limited to early life, and (c) beginning in young adult life. Hormone-sensitive lipolysis is lost with age. Food restriction from 6 weeks of age prevents this loss, and food restriction started in adult life causes the recovery of this lipolysis. Hormone binding studies reveal that: (a) changes in glucagon-stimulated lipolysis parallel changes in glucagon binding; (b) glucagon binding and glucagon-stimulated lipolysis correlate inversely with cell size; (c) changes in epinephrine-stimulated lipolysis are not due to changes in beta-adrenergic binding; and (d) neither beta-adrenergic binding nor epinephrine-promoted lipolysis correlate with fat cell size. Phosphodiesterase activity is not influenced by diet, making it unlikely to be a postreceptor component lost with age.
authors.affiliation: Department of Physiology, University of Texas Health Science Center at San Antonio.
authors.forename: H A
authors.initials: HA
authors.lastname: Bertrand
authors.forename: W R
authors.initials: WR
authors.lastname: Anderson
authors.forename: E J
authors.initials: EJ
authors.lastname: Masoro
authors.forename: B P
authors.initials: BP
authors.lastname: Yu
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D002478:Cells, Cultured; D004082:Dihydroalprenolol / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism / Q000494:pharmacology; D005508:Food Deprivation / Q000502:physiology*; D005934:Glucagon / Q000378:metabolism / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D009929:Organ Size; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms
chemical_list: D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D005934:Glucagon; D010727:Phosphoric Diester Hydrolases; D004837:Epinephrine