Rapid, sensitive detection of plasma IL-1 by extraction and bioassay.
A method for separating IL-1 from plasma inhibitors by silica extraction has been developed and coupled to a highly sensitive bioassay using the LBRM TG6 cell line and the I1-2 dependent HT2A cell line. Using this assay we have detected IL-1 activity in plasma from patients undergoing elective surgery.
Series
J J
JJ
Department of Biochemistry, Royal Infirmary, Glasgow, U.K.
Shapiro
D
D
Cameron
I
I
Smith
J
J
Fraser
W D
WD
Garden
O J
OJ
Shenkin
A
A
D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D006801:Humans; D007375:Interleukin-1 / Q000097:blood* / Q000302:isolation & purification; D008223:Lymphoma / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*; D012822:Silicon Dioxide; D013601:T-Lymphocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D012822:Silicon Dioxide