A multi-copy gene encodes a potentially protective antigen in Brugia malayi.
A genomic library of Brugia malayi was constructed and screened by hybridization with a cDNA clone corresponding to a potentially protective antigen of 63 kDa. The antigen is encoded by a multicopy gene family. Five distinct gene copies were isolated and one was characterized in detail by nucleotide sequence analysis. An apparent pseudogene was also characterized. The organization of genes encoding the antigen is typical of higher eukaryotes in exon/intron organization although the introns have an unusually high A+T content (75%). Organization of the genomic sequence along with S1 nuclease and primer extension analyses indicate that a short untranslated exon is spliced to the 5' end of the mRNAs encoding the antigen.
Perrine
K G
KG
Case Western Reserve University, School of Medicine, Department of Molecular Biology and Microbiology, Cleveland, OH 44106.
Denker
J A
JA
Nilsen
T W
TW
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D010582:Bacteriophage lambda; D001483:Base Sequence; D002009:Brugia / Q000235:genetics*; D002874:Chromosome Mapping; D004247:DNA; D004262:DNA Restriction Enzymes; D005091:Exons; D005809:Genes, Regulator; D007438:Introns; D008842:Microfilariae / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D011544:Pseudogenes
D000947:Antigens, Helminth; D004247:DNA; D004262:DNA Restriction Enzymes