title: Isolation and primary structure of the gene encoding fructose-1,6-bisphosphatase from Saccharomyces cerevisiae.
abstract: The gene encoding Saccharomyces cerevisiae fructose-1,6-bisphosphatase (FBP1) was isolated. Constructed fbp1::HIS3 null mutants were unable to grow with ethanol, and growth was restored after transformation with the cloned fbp gene. The gene codes for a protein of 347 amino acid residues with an Mr of 38131. Homology with the pig kidney cortex and the sheep liver enzyme is 47.7% and 46.6%, respectively, within a central core of 328 amino acid residues. The cloned promoter size was 318 bp and allowed only low level expression of the gene. This indicates a positive activation site (UAS) upstream of the cloned DNA fragment.
authors.affiliation: Medizinisch-Naturwissenschaftliches Forschungszentrum, Universität Tübingen, FRG.
authors.forename: K D
authors.initials: KD
authors.lastname: Entian
authors.forename: R F
authors.initials: RF
authors.lastname: Vogel
authors.forename: M
authors.initials: M
authors.lastname: Rose
authors.forename: L
authors.initials: L
authors.lastname: Hofmann
authors.forename: D
authors.initials: D
authors.lastname: Mecke
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D004262:DNA Restriction Enzymes; D006597:Fructose-Bisphosphatase