Plasmid typing of Shigella sonnei epidemic strains and molecular relationship of their R-plasmids.
We conducted a surveillance program on epidemic and/or endemic Shigella strains in Asturias (Spain), their frequency and dispersion in our community, and their R-plasmids. We analyzed initial isolates of Shigella sonnei from two epidemic outbreaks using antibiotic resistance patterns and plasmid profile analysis as epidemiological markers. We found that the 2 outbreaks were caused by different S. sonnei strains, which respectively carried one and two R-plasmids together with other plasmids. The molecular relationship among these and three other R-plasmids from two S. sonnei strains isolated during a previous outbreak, were studied by restriction enzyme analysis and DNA-DNA hybridizations. We were able to establish different levels of relationship among the six R-plasmids.
Mendoza
M C
MC
Departamento de Biologia Functional, Universidad de Oviedo, Spain.
Gonzalez
A J
AJ
Mendez
F J
FJ
Hardisson
C
C
D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D004196:Disease Outbreaks; D004352:Drug Resistance, Microbial; D004405:Dysentery, Bacillary / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011815:R Factors; D012764:Shigella sonnei / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D013030:Spain
D004279:DNA, Viral; D004262:DNA Restriction Enzymes