Bone marrow derived macrophages have polyamine and ectoenzyme phenotypes distinct from resident macrophages.
Several prototype macrophage (MO) populations were compared for differences in ectoenzyme phenotype and polyamine content. Resident peritoneal MO and Corynebacterium parvum (CP)-activated peritoneal MO expressed unique ectoenzyme phenotypes, while bone marrow derived MO (BMDMO), obtained from stem cells after 7 days in culture with colony stimulating factor, and thioglycollate (TG)-elicited peritoneal MO exhibited a similar ectoenzyme phenotype. All of the MO populations, however, differed in polyamine accumulation patterns. These results suggest that ectoenzyme phenotypes do not serve as completely selective markers of MO differentiation. Moreover, BMDMO do not resemble steady state tissue peritoneal MO but appear to resemble inflammatory MO in several respects. Therefore activated BMDMO do not appear to provide an accurate model system for their continued use in studies to characterize the development of resident tissue MO.
Dempsey
W L
WL
Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129.
Hwu
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P
Russell
D H
DH
Morahan
P S
PS
D015720:5'-Nucleotidase; D000818:Animals; D001854:Bone Marrow Cells; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005260:Female; D007700:Kinetics; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000201:enzymology* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009708:Nucleotidases / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010641:Phenotype; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D011425:Propionibacterium acnes; D013864:Thioglycolates / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D011073:Polyamines; D013864:Thioglycolates; D009708:Nucleotidases; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; D007931:Leucyl Aminopeptidase