v-myb dominance over v-myc in doubly transformed chick myelomonocytic cells.
Chick myelomonocytic cells transformed by the v-myc oncogene resemble mature macrophages; those transformed by v-myb or v-myb,ets exhibit an immature phenotype. We have analyzed whether these oncogenes are capable of altering the differentiation phenotype of transformed cells by introducing both v-myc plus either v-myb or v-myb,ets into the same cells. Surprisingly, the doubly transformed cells were found to be essentially indistinguishable from cells transformed by v-myb or v-myb,ets alone even when they expressed a high level of v-myc protein. These results demonstrate that v-myb is dominant over v-myc and that, while v-myc induces cell proliferation without affecting differentiation, v-myb induces in the same target cells both proliferation and a block or reversal of differentiation.
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Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Beug
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Graf
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D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002645:Chickens; D006098:Granulocytes / Q000166:cytology; D008264:Macrophages / Q000166:cytology*; D009000:Monocytes / Q000166:cytology; D009856:Oncogene Proteins, Viral / Q000235:genetics; D009857:Oncogenes; D010641:Phenotype
D009856:Oncogene Proteins, Viral