Ileal vasoactive intestinal peptide (VIP) levels and VIP receptor/effector system in ileal epithelial cells after colectomy in the rat.
The interaction of VIP (binding to specific receptors and stimulation of cyclic AMP accumulation) with ileal epithelial cells and the levels of the neuropeptide in the ileal segment were determined after colectomy (removal of cecum and colon followed by ileorectal anastomosis) in the rat. The number of VIP receptors (but not the affinity) and the efficiency (but not the potency) of the neuropeptide upon stimulation of cyclic AMP accumulation in ileal epithelial cells increased 21 (but not 7) days after colectomy, whereas VIP ileal levels followed an inverse pattern. These changes could be interpreted in terms of a consequence or a cause of some of the phenomena that appear after colectomy, i.e., chronic watery diarrhea.
Fernandez-Moreno
M D
MD
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
Fernandez-Gonzalez
M A
MA
Arilla
E
E
Lorenzo
M J
MJ
Prieto
J C
JC
D000818:Animals; D003082:Colectomy; D000242:Cyclic AMP / Q000378:metabolism; D004848:Epithelium / Q000032:analysis; D005260:Female; D007082:Ileum / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis*; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide / Q000032:analysis* / Q000494:pharmacology
D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP