Defective dendritic cell accessory function in NZB/W F1 hybrid mice.
The proliferative response of NZB/W F1 hybrid mice when activated by sodium periodate (NaIO4) was found to be markedly defective as compared to the response in control NZW mice. This defect was observed in both 4 month old female mice and in 8-10 month old male mice. To determine whether the defect was an intrinsic T cell defect or an accessory cell defect, splenic dendritic cells (DC) were purified and their ability to activate enriched T cells after NaIO4 stimulation was assessed. In mixing experiments it was observed that normal NZW dendritic cells could restore the response of NZB/W F1 hybrid T cells whereas addition of NZB/W F1 dendritic cells to NZW T cells resulted in defective 3H-thymidine incorporation after NaIO4 stimulation. These results indicate that accessory DC of NZB/W F1 mice are defective and unable to support T cell responses to the mitogen NaIO4.
Phillips
R
R
Medical Research Council Human Cellular Immunology Unit, School of Pathology, South African Institute for Medical Research, Johannesburg.
Lomnitzer
R
R
Rabson
A R
AR
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006824:Hybridization, Genetic; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D010504:Periodic Acid / Q000494:pharmacology
D010504:Periodic Acid; C009288:metaperiodate