Adenosine-guanosine preferential photocleavage of DNA by azido-benzoyl- and diazocyclopenta-dienylcarbonyloxy derivatives of 9-aminoacridine.
The photoreactions of 9-[6-(4-azidobenzamido)hexylamino]acridine (AHA) and 9-[6-(2-diazocyclopentadienylcarbonyloxy)hexylamino]acridine (DHA) with double stranded DNA result in formation of single strand nicks and alkali labile sites (adducts) with an efficiency of 6 x 10(-3) nicks per AHA and 3 x 10(-2) nicks per DHA molecule. The alkali dependent DNA cleavage by AHA shows a pronounced A+G preference whereas that by DHA is practically sequence independent. In the presence of diacridines, however, DHA exhibits a preference for cleavage at guanosines. These DNA photocleaving reagents could be useful for DNA photofootprinting and photosequencing.
Nielsen
P E
PE
Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
Jeppesen
C
C
Egholm
M
M
Buchardt
O
O
D000241:Adenosine; D000609:Aminoacridines / Q000494:pharmacology*; D001386:Azides / Q000494:pharmacology; D001483:Base Sequence; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004262:DNA Restriction Enzymes; D006151:Guanosine; D007700:Kinetics; D008027:Light; D010782:Photolysis; D010957:Plasmids; D014466:Ultraviolet Rays
D000609:Aminoacridines; D001386:Azides; D006151:Guanosine; C056129:9-(6-(2-diazocyclopentadienylcarbonyloxy)hexylamino)acridine; C056128:9-(6-(4-azidobenzamido)hexylamino)acridine; D004247:DNA; D004262:DNA Restriction Enzymes; D000241:Adenosine