Group II intron self-splicing. Alternative reaction conditions yield novel products.
Reaction parameters were modified to enhance the in vitro reaction rate and to reveal partial and novel reactions of the group II intron 5g of the mitochondrial gene from Saccharomyces cerevisiae encoding cytochrome c oxidase subunit I. One alteration yields separate 5'- and 3'-exons plus linear excised intron as the main products. A linear reaction intermediate, containing intron and 3'-exon, and products resulting from cleavages at two unexpected sites were identified. Spliced exon "reopening," a novel reaction between excised intron and spliced exons, appears responsible for separate 5'- and 3'-exon products.
Jarrell
K A
KA
Department of Molecular Genetics, Ohio State University, Columbus, 43210.
Peebles
C L
CL
Dietrich
R C
RC
Romiti
S L
SL
Perlman
P S
PS
D000645:Ammonium Sulfate / Q000494:pharmacology; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005091:Exons; D005800:Genes, Fungal; D007438:Introns; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D011189:Potassium Chloride / Q000494:pharmacology; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D012331:RNA, Fungal; D015636:Magnesium Chloride; D012965:Sodium Chloride; D011189:Potassium Chloride; D003576:Electron Transport Complex IV; D008274:Magnesium; D000645:Ammonium Sulfate