The role of DNA sequence in the formation of Z-DNA versus cruciforms in plasmids.
The capacities of four synthetic sequences containing runs of perfectly alternating purine-pyrimidine base pairs (bp) to adopt left-handed structures were evaluated in a homologous family of recombinant plasmids. All the sequences had the same G+C content (50%) and consisted of simple tetranucleotide repeat units but differed in the relative orientations of these units. For some of the sequences, several alternate secondary structures were theoretically possible; a variety of probes (S1 nuclease, bromoacetaldehyde, OsO4, T7 gene 3 endonuclease, supercoil-induced gel relaxation studies) under a wide range of reaction conditions was used to determine which structures were adopted as a function of superhelical stress. The precise positions at the bp level of reactions with these chemical and enzymatic probes were determined. We conclude that for short (20-24 bp) sequences containing runs of alternating (T-G) and (C-A), the cruciform state is preferred over the similarly allowable left-handed form provided that symmetry constraints allow. However, these sequences can be induced to form a left-handed helix under appropriate conditions. This is the first demonstration of plasmid inserts which will adopt more than one unusual DNA structure in response to negative superhelical stress. The structural properties of a molecule containing a Z-Z junction were studied, and we conclude that the disruption caused by this feature extends over only a few bp although it requires a high energetic penalty.
McLean
M J
MJ
Department of Biochemistry, School of Medicine, University of Alabama, Birmingham 35294.
Wells
R D
RD
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D010957:Plasmids
D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes