title: Interaction of the MvaI restriction enzyme with synthetic DNA fragments.
abstract: The cleavage of synthetic DNA duplexes by the restriction endonuclease MvaI has been studied. The main result of the cleavage experiments is that MvaI cleaves unmodified duplexes in two single strand scissions in separate events and that the two strands are cleaved at significantly different rates. One strand nicks within the recognition site do not affect the cleavage. Furthermore, neither a pyrophosphate internucleotide bond modification in one strand nor the absence of one phosphate group at the central dA-residue of the recognition site do inhibit the cleavage of the second strand.
authors.affiliation: Department of Chemistry, Humboldt University, Berlin, GDR.
authors.forename: C D
authors.initials: CD
authors.lastname: Pein
authors.forename: D
authors.initials: D
authors.lastname: Cech
authors.forename: E S
authors.initials: ES
authors.lastname: Gromova
authors.forename: T S
authors.initials: TS
authors.lastname: Orezkaya
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
authors.forename: E A
authors.initials: EA
authors.lastname: Kubareva
mesh_terms: D001483:Base Sequence; D004247:DNA; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D013379:Substrate Specificity
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific