title: Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
abstract: Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB (96.5') and oriC (84') in the published physical map of E. coli. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, UK.
authors.forename: V
authors.initials: V
authors.lastname: Knott
authors.forename: D J
authors.initials: DJ
authors.lastname: Rees
authors.forename: Z
authors.initials: Z
authors.lastname: Cheng
authors.forename: G G
authors.initials: GG
authors.lastname: Brownlee
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D003360:Cosmids; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis
chemical_list: D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes