Solubilization and characterization of the chicken oocyte vitellogenin receptor.
This paper describes the biochemical characterization of the chicken oocyte plasma-membrane receptor for one of the major lipid-carrying yolk proteins, vitellogenin (VTG). The receptor was extracted from oocyte membranes with the non-ionic detergent octyl-beta-D-glucoside and visualized by ligand blotting, with 125I-VTG as a protein with an apparent Mr of 96000, under non-reducing conditions. It exhibited high affinity for native chicken VTG (Kd 2 X 10(-7) M) but was unable to bind VTG with reductively methylated lysine residues or phosvitin (the phosphoserine-rich intracellular cleavage product of VTG). Polyclonal antibodies to the 96 kDa protein inhibited VTG binding to the receptor and were able to precipitate functional VTG-receptor activity from oocyte-membrane detergent extracts with a concomitant removal of the 96 kDa protein. Antibodies directed against the mammalian receptor for low-density lipoprotein showed cross-reactivity with the chicken oocyte VTG receptor, raising the possibility that lipoprotein receptors in birds are structurally related to those in mammalian species.
Stifani
S
S
Department of Biochemistry, University of Alberta, Edmonton, Canada.
George
R
R
Schneider
W J
WJ
D000818:Animals; D000906:Antibodies; D011232:Chemical Precipitation; D002645:Chickens; D003902:Detergents; D004527:Egg Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005960:Glucosides; D008024:Ligands; D008074:Lipoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology; D009865:Oocytes / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D014819:Vitellogenins / Q000302:isolation & purification / Q000378:metabolism*
D000906:Antibodies; D003902:Detergents; D004527:Egg Proteins; D005960:Glucosides; D008024:Ligands; D008074:Lipoproteins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D014819:Vitellogenins; C030474:vitellogenin receptor; C018619:octyl-beta-D-glucoside