Determination of inorganic tin in biological samples by hydride generation-atomic absorption spectrometry after silica gel cleanup.
A method is described for the determination of inorganic tin in biological samples by hydride generation-atomic absorption spectrometry (HG-AAS). A sample is extracted with ethyl acetate after addition of HCl and NaCl. The concentrated extract is passed through a silica gel column. The column is washed with ethanol, water, and 0.2N HCl successively, and then inorganic tin is eluted with 2N HCl and measured by HG-AAS. Recoveries from fish muscle spiked with 0.1 micrograms/g Sn4+ are 78.9 +/- 4.2% (average +/- standard deviation, n = 5). The detection limit is 0.01 micrograms/g as Sn.
Tsuda
T
T
Shiga Prefectural Institute of Public Health and Environmental Science, Japan.
Wada
M
M
Aoki
S
S
Matsui
Y
Y
D000818:Animals; D005399:Fishes / Q000378:metabolism*; D007202:Indicators and Reagents; D009132:Muscles / Q000032:analysis; D009947:Organotin Compounds / Q000032:analysis; D012822:Silicon Dioxide; D013054:Spectrophotometry, Atomic; D014001:Tin / Q000032:analysis*
D007202:Indicators and Reagents; D009947:Organotin Compounds; D014001:Tin; D012822:Silicon Dioxide