Natural abundance 13C-NMR study of paramagnetic horse heart ferricytochrome c cyanide complex: assignment of hyperfine shifted heme methyl carbon resonances.
Hyperfine shifted heme methyl carbon resonances of paramagnetic horse heart ferricytochrome c cyanide complex (Cyt-c(CN)) have been observed for the first time in the natural abundance 13C-NMR spectrum and assigned using 1H-13C heteronuclear chemical shift correlated spectroscopy (1H-13C COSY). Individual heme methyl carbon NMR signal assignment permits a direct comparison between the hyperfine shifts of heme methyl carbon and attached methyl proton resonances which provides a useful information on the delocalization mechanism of the unpaired spin from the pi-conjugated system of porphyrin ring into the peripheral methyl side chains.
Yamamoto
Y
Y
Department of Polymer Chemistry, Tokyo Institute of Technology, Japan.
Nanai
N
N
Inoue
Y
Y
Chûjô
R
R
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003486:Cyanides / Q000032:analysis*; D003574:Cytochrome c Group / Q000032:analysis*; D006418:Heme / Q000032:analysis*; D006427:Hemin / Q000032:analysis; D006736:Horses; D009682:Magnetic Resonance Spectroscopy; D011522:Protons
D003486:Cyanides; D003574:Cytochrome c Group; D011522:Protons; D006418:Heme; D006427:Hemin