Nebulin cDNAs detect a 25-kilobase transcript in skeletal muscle and localize to human chromosome 2.
By virtue of the protein's size, myofibrillar localization, and proposed functional role, the gene encoding the giant sarcomere matrix protein nebulin represents a possible site for myopathic mutations. Using polyclonal anti-nebulin antisera to screen a cDNA expression library, we have isolated and characterized two separate human fetal muscle cDNA clones. By recovering fusion polypeptide-bound portions of our polyclonal antiserum and reutilizing them to probe Western blots, we further demonstrate that the expressed cDNAs encode polypeptide epitopes unique to the protein nebulin. Both cDNAs detect a 25-kb skeletal muscle RNA transcript and localize to human chromosome 2. The identification of nebulin cDNA clones enables the complete analysis of this enormous mRNA by transcript walking through muscle cDNA libraries. Here we report a restriction map of the 3' end of the human nebulin transcript, with reference to the genomic fragments identified by the cDNA.
Stedman
H
H
Department of Human Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104.
Browning
K
K
Oliver
N
N
Oronzi-Scott
M
M
Fischbeck
K
K
Sarkar
S
S
Sylvester
J
J
Schmickel
R
R
Wang
K
K
D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000378:metabolism*; D009136:Muscular Dystrophies / Q000235:genetics; D009693:Nucleic Acid Hybridization; D014158:Transcription, Genetic
D009124:Muscle Proteins; C035620:nebulin; D004247:DNA; D004262:DNA Restriction Enzymes