title: Vaccines against Aujeszky's disease: comparison of efficacy, DNA fingerprints and antibody response to glycoprotein I.
abstract: The efficacy of two inactivated and three attenuated Aujeszky's disease virus (ADV) vaccines was compared in pigs in a standardised test. After vaccination, excretion of attenuated vaccine viruses was not detected. All vaccines were capable of preventing mortality, but none conferred complete protection against challenge with a virulent strain of ADV 3 months after the first vaccination. After a single vaccination, no large differences in vaccine efficacy were observed. Revaccination markedly enhanced protection; however, with one vaccine this was not the case. The two inactivated vaccines induced quite different levels of neutralising antibodies. Two vaccine viruses had a wild-type DNA fingerprint and gave rise to an antibody response to glycoprotein I (gI) of ADV. The three remaining vaccines carried a deletion in the unique short region of the genome. Two of them failed to induce gI-antibody in pigs. Pigs given the third 'deleted' vaccine did not respond uniformly of gI. With regard to control of Aujeszky's disease, efficacious vaccines with a 'serological' marker may be preferred in the future.
authors.affiliation: Central Veterinary Institute, Department of Virology, Lelystad, The Netherlands.
authors.forename: J T
authors.initials: JT
authors.lastname: van Oirschot
authors.forename: A L
authors.initials: AL
authors.lastname: Gielkens
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D001831:Body Temperature; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology*; D009693:Nucleic Acid Hybridization; D011557:Pseudorabies / Q000517:prevention & control*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000009:adverse effects / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000009:adverse effects / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI