Double labeling of SIgG+ cells harboring a lymphotropic herpesvirus by avidin-biotin complex immunoperoxidase and immunogold-silver staining techniques.
By reversing the usual order of double-staining procedures, we obtained optimal conditions for simultaneous detection of guinea pig lymphotropic herpesvirus (GPHLV) and surface immunoglobulin G-positive (SIgG+) cells in paraffin-embedded tissue sections. Of the different fixatives tested, Bouin's solution gave the best preservation of morphology and cell surface IgG. Double immunolabeling was best achieved when avidin-biotin complex immunoperoxidase (ABC-IP) staining was performed first for detection of intracellular viral antigen, followed by immunogold-silver staining (IGSS) for localization of cell surface IgG.
Nagy-Oltvai
Z
Z
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut.
Brady
T
T
Hsiung
G D
GD
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D005260:Female; D006168:Guinea Pigs; D006564:Herpesviridae / Q000276:immunology / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology / Q000382:microbiology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013154:Spleen / Q000276:immunology / Q000382:microbiology
D000956:Antigens, Viral; D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell