title: Dual regulation of neutrophil adenylate cyclase by fluoride and its relationship to cellular activation.
abstract: 1 Fluoride stimulated (1-10 mM) and inhibited (10-100 mM) adenylate cyclase of neutrophil membranes in a GTP-independent manner. The latter fluoride concentration range corresponded to that shown previously to induce cellular responses. 2 Dual regulation of cyclase activity was also exhibited by a nonhydrolysable GTP analogue, guanosine 5'[gamma-thio]triphosphate (GTP gamma S). Inhibition was observed at 0.1-10 nM GTP gamma S while stimulation occurred at greater than 10 nM GTP gamma S. 3 Relatively high levels (greater than microM) of formylmethionyl-leucyl-phenylalanine inhibited adenylate cyclase in the presence of GTP (10 microM). 4 Pertussis toxin pretreatment abolished adenylate cyclase inhibition mediated by GTP gamma S and formylmethionyl-leucyl-phenylalanine but did not influence fluoride-induced inhibition.
authors.affiliation: Department of Pharmacology, University of Alberta, Edmonton, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Saad
authors.forename: C F
authors.initials: CF
authors.lastname: Strnad
authors.forename: K
authors.initials: K
authors.lastname: Wong
mesh_terms: D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000097:blood*; D005576:Colforsin / Q000494:pharmacology; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000201:enzymology*; D037342:Pertussis Toxin; D012969:Sodium Fluoride / Q000494:pharmacology*; D013481:Superoxides / Q000097:blood; D013873:Thionucleotides / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D037361:Adenylate Cyclase Toxin; D013873:Thionucleotides; D010566:Virulence Factors, Bordetella; D013481:Superoxides; D005576:Colforsin; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D009240:N-Formylmethionine Leucyl-Phenylalanine; D006160:Guanosine Triphosphate; D012969:Sodium Fluoride; D037342:Pertussis Toxin; D000262:Adenylyl Cyclases