Swiveling and decatenation of replicating simian virus 40 genomes in vivo.
We have found that type II topoisomerase inhibitors have two effects on replicating simian virus 40 genomes in vivo: production of catenated dimers and slowed replication of the last 5% of the genome. This suggests that type II topoisomerase simultaneously decatenates and facilitates replication fork movement at this stage of DNA replication. On the basis of this observation, a detailed model is proposed for the roles of topoisomerases I and II in simian virus 40 DNA replication.
Snapka
R M
RM
Department of Radiology, Ohio State University, Columbus 43210.
Powelson
M A
MA
Strayer
J M
JM
D000818:Animals; D002166:Camptothecin / Q000494:pharmacology; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D005047:Etoposide / Q000494:pharmacology; D005814:Genes, Viral / Q000187:drug effects; D007364:Intercalating Agents / Q000494:pharmacology; D013539:Simian virus 40 / Q000235:genetics*; D013713:Teniposide / Q000494:pharmacology; D059005:Topoisomerase II Inhibitors; D014779:Virus Replication
D004279:DNA, Viral; D007364:Intercalating Agents; D059005:Topoisomerase II Inhibitors; D005047:Etoposide; D013713:Teniposide; D002166:Camptothecin