Effect of anti-insulin receptor antibodies on insulin-sensitive phosphodiesterase in rat fat cells.
Effect of sera with anti-insulin receptor antibodies (AIRS) on insulin-sensitive phosphodiesterase in rat fat cells was examined. AIRS activated the enzyme when incubated with intact fat cells. AIRS (1:400 dilution) were less potent for activation of the phosphodiesterase than insulin (3 nM), but were more potent for inhibition of 125I-insulin binding to fat cells than insulin. When insulin receptor of fat cells was destroyed with trypsin-treatment, AIRS as well as insulin completely lost the ability to activate the phosphodiesterase. These findings suggest that AIRS bind to or very near the insulin receptor and exhibit insulin-like biological effect of the phosphodiesterase activation.
Suzuki
T
T
Division of Endocrinology and Metabolism, Asahi Life Foundation, Tokyo, Japan.
Makino
H
H
Kanatsuka
A
A
Yoshida
S
S
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000276:immunology*
D001323:Autoantibodies; D007328:Insulin; D007457:Iodine Radioisotopes; D011972:Receptor, Insulin; D010727:Phosphoric Diester Hydrolases