Synthesis of human rotavirus polypeptides in cell culture.
The replication strategy of a human serotype 1 rotavirus, adapted to rapid growth in CV-1 cells, was investigated. A single cycle growth curve revealed eclipse and latent periods of 3 and 4 hours, respectively. Although the extent of reduction of host cell protein synthesis was directly related to the multiplicity of infection of the virus, incorporation of actinomycin D and excess NaCl into the medium resulted in significant reduction in host cell background and enabled observation of viral polypeptides as early as 2 hours post infection. Five polypeptides were found to be structural components of the virion, and a further eight appeared to be nonstructural proteins or intermediates. Five polypeptides were glycosylated during virus replication, but only one of these, VP7, was a definite structural glycoprotein. Pulse-chase experiments revealed that four low molecular weight polypeptides underwent post-translational modifications.
Heath
R L
RL
Virology Department, Fairfield Hospital, Victoria, Australia.
Birch
C J
CJ
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D006023:Glycoproteins / Q000096:biosynthesis; D007700:Kinetics; D010452:Peptide Biosynthesis; D010455:Peptides / Q000032:analysis; D012401:Rotavirus / Q000254:growth & development / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*; D015678:Viral Structural Proteins
D006023:Glycoproteins; D010455:Peptides; D014764:Viral Proteins; D015678:Viral Structural Proteins