title: BcefI, a new type IIS restriction endonuclease.
abstract: A new Type IIS restriction endonuclease was identified, partially purified and characterized from a Bacillus cereus subsp. fluorescens strain. The enzyme recognizes the nonpalindromic sequence ACGGC and cleaves at a distance from it. The cleavage appears to occur with a +/- 1 basepair uncertainty. Thus the cleavage and recognition site is as shown below: ACGGC(N)11-13 TGCCG(N)12-14.
authors.affiliation: Institute of Biochemistry, Biological Research Center, Szeged, Hungary.
authors.forename: P
authors.initials: P
authors.lastname: Venetianer
authors.forename: A
authors.initials: A
authors.lastname: Orosz
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004262:DNA Restriction Enzymes; C039566:CGCG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific