Use of various mitogens and cell stimulation index in 21 patients with chronic lymphocytic leukaemia.
A number of mitogens was used in 21 chronic B-lymphocytic leukaemia patients. Thymidine uptake assays were performed to evaluate cell stimulation. Phytohemagglutinin and phorbol-myristate 13 acetate were found to be the most efficient on cell proliferation. Abnormal clones were found 7 times with PMA, 4 times with PHA, twice with pokeweed, but in no case with lipopolysaccharide or proteine-A. The efficiency of PHA as a B-cell activator is likely to be due to T cell mediation.
Mossafa
H
H
Département d'Hématologie et Oncologie Médicale, CNRS URA, Hôpital Jean Bernard, Poitiers, France.
Huret
J L
JL
Mossalayi
M D
MD
Brizard
A
A
Tanzer
J
J
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000187:drug effects / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects / Q000473:pathology; D004357:Drug Synergism; D005260:Female; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology
D008934:Mitogens