Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB (96.5') and oriC (84') in the published physical map of E. coli. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.
Knott
V
V
Sir William Dunn School of Pathology, University of Oxford, UK.
Rees
D J
DJ
Cheng
Z
Z
Brownlee
G G
GG
D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D003360:Cosmids; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis
D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes