[Analysis of immunoregulatory peptides of the thymus using high performance liquid chromatography].
High performance liquid chromatography (gel filtration and chromatography in the reverse phase) was applied to analyse a composition of the heterogenous immunoregulating peptide preparation T-activine isolated from calf thymus. Gel chromatography at high pressure on the column TSK G2000 SW enabled to separate T-activine into 3 subfractions at a preparative scale. Physico-chemical properties and biological activity of these fractions were studied in vitro. Subfraction B with molecular mass of 1.0-3.5 kD and pI below 5.9 proved to be the most active.
Korotkova
M N
MN
Sanina
I V
IV
Arion
V Ia
VIa
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D010455:Peptides / Q000032:analysis*; D013947:Thymosin / Q000032:analysis*; D013949:Thymus Extracts; D013950:Thymus Gland / Q000032:analysis*
D010455:Peptides; D013949:Thymus Extracts; D013947:Thymosin; C036191:T-activin
Analiz immunoreguliatornykh peptidov timusa metodom vysokoéffektivnozhidkostnoĭ khromatografii.