Effect of growth temperature on membrane dynamics in a thermophilic cyanobacterium: a spin label study.
A strain of Synechococcus sp. was grown at its optimal growth temperature (58 degrees C) and at 38 degrees C, in order to investigate possible adaptations of membrane-related properties to growth temperature. Light-induced electron transport in thylakoid membranes from both types of cells showed linear Arrhenius plots with the same activation energy (48 kJ/mol). Membranes from cells grown at 58 degrees C had a higher temperature optimum (53 degrees C) than those from cells grown at 38 degrees C (41 degrees C). Growth at 38 degrees C caused an increase in the proportion of unsaturated fatty acids compared to growth at 58 degrees C. The fluidity of the membranes was investigated by measuring the temperature dependence of the parameters derived from electron spin resonance spectra of the spin-labels 5-doxyldecane, 5-doxylstearate and 16-doxylstearate. Only small differences between the dynamic properties of the membranes from cells grown at different temperatures could be detected. This suggests that the observed change in fatty acid composition of the membranes following the change in growth temperature does not serve to maintain a constant viscosity at the growth temperature.
Miller
M
M
Institute of Biochemistry, Odense University, Denmark.
Pedersen
J Z
JZ
Cox
R P
RP
D002338:Carotenoids / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D002734:Chlorophyll / Q000032:analysis; D002855:Chromatography, Thin Layer; D000458:Cyanobacteria / Q000032:analysis / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D005227:Fatty Acids / Q000032:analysis; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis; D013113:Spin Labels; D013696:Temperature; D014783:Viscosity
D005227:Fatty Acids; D008563:Membrane Lipids; D008565:Membrane Proteins; D013113:Spin Labels; D002734:Chlorophyll; D002338:Carotenoids; D010100:Oxygen