[Preliminary study on the nucleic acid hybridization technic in detecting HSV-2 DNA corresponding sequence of cancer tissues of the uterine cervix].
In this paper, 34 samples, 25 from 18 cervical carcinoma patients and 9 from 8 non-cervical carcinoma patients, were assayed using (alpha-32P) dCTP HSV-2 DNA probe and nucleic acid hybridization technic. It was shown that the positive rates of HSV-2 DNA corresponding sequence were 50% (2/4), 36% (5/14) and 12% (1/8) in the in situ cancer, invasive cancer and non-cervical cancer tissues. No HSV was isolated from 7 cancer specimens which showed a positive reaction by nucleic acid hybridization.
Cui
H Y
HY
Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing.
Zhang
W
W
Li
J
J
Liu
B Y
BY
Li
J S
JS
Lin
Y C
YC
Zhang
W H
WH
Wu
A R
AR
Liu
Z M
ZM
D000818:Animals; D001483:Base Sequence; D002278:Carcinoma in Situ / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007668:Kidney / Q000032:analysis; D008297:Male; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000032:analysis*
D004273:DNA, Neoplasm; D004279:DNA, Viral