title: Detection of Chlamydia trachomatis by in situ hybridization with sulphonated total DNA.
abstract: In situ nucleic acid hybridization was applied to the detection of Chlamydia trachomatis on microscope slides by use of sulphonated total DNA as a probe. Visualization of labelled DNA was obtained using a commercial enzyme-linked monoclonal antibody. A mixture of paraformaldehyde and glutaraldehyde was found to be the best fixative. With high probe concentration (10 micrograms/ml), intracellular inclusions were detected as early as 8 h after inoculating the cell culture. Extracellular elementary bodies could also be detected. Five genital specimens were tested by in situ hybridization; the results were in agreement with those observed by culture.
authors.affiliation: Laboratoire de Bactériologie, Hôpital Pellegrin, Bordeaux, France.
authors.forename: B
authors.initials: B
authors.lastname: Dutilh
authors.forename: C
authors.initials: C
authors.lastname: Bébéar
authors.forename: D
authors.initials: D
authors.lastname: Taylor-Robinson
authors.forename: P A
authors.initials: PA
authors.lastname: Grimont
mesh_terms: D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D003596:Cytosine; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005260:Female; D005404:Fixatives; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure; D008297:Male; D009693:Nucleic Acid Hybridization; D013447:Sulfites
chemical_list: D004269:DNA, Bacterial; D005404:Fixatives; D013447:Sulfites; D003596:Cytosine; C009279:sodium bisulfite