title: The chloroplast tRNALys(UUU) gene from mustard (Sinapis alba) contains a class II intron potentially coding for a maturase-related polypeptide.
abstract: The trnK gene endocing the tRNALys(UUU) has been located on mustard (Sinapis alba) chloroplast DNA, 263 bp upstream of the psbA gene on the same strand. The nucleotide sequence of the trnK gene and its flanking regions as well as the putative transcription start and termination sites are shown. The 5' end of the transcript lies 121 bp upstream of the 5' tRNA coding region and is preceded by procaryotic-type "-10" and "-35" sequence elements, while the 3' end maps 2.77 kb downstream to a DNA region with possible stemloop secondary structure. The anticodon loop of the tRNALys is interrupted by a 2,574 bp intron containing a long open reading frame, which codes for 524 amino acids. Based on conserved stem and loop structures, this intron has characteristic features of a class II intron. A region near the carboxyl terminus of the derived polypeptide appears structurally related to maturases.
authors.affiliation: Arbeitsgruppe Pflanzliche Zellphysiologie, Ruhr-Universität Bochum, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Neuhaus
authors.forename: G
authors.initials: G
authors.lastname: Link
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004722:Endoribonucleases; D005796:Genes; D007438:Introns; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000201:enzymology / Q000235:genetics; D009690:Nucleic Acid Conformation; D009713:Nucleotidyltransferases / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D010946:Plants, Medicinal; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012357:RNA, Transfer, Lys / Q000235:genetics*
chemical_list: D012345:RNA, Transfer, Amino Acid-Specific; D012357:RNA, Transfer, Lys; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases