Functional nuclear suppressor of mitochondrial oxi2 mutations in yeast.
A semidominant nuclear suppressor, called nam6, of oxi2-V276 mitochondrial mutation has been isolated and characterized. The nuclear character of nam6 was proved by its retention in rho degree strains, lack of mitotic segregation in diploids and meiotic 2:2 segregation in tetrads. The specificity of nam6 was tested on 315 mit- mutations of four mitochondrial genes (oxi1, oxi2, oxi3, and cob-box). It suppresses clearly only three mutations in the oxi2 gene, restoring partially or completely cytochrome aa3 formation. The results suggest a functional character of the suppression.
Kruszewska
A
A
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
Szcześniak
B
B
D000483:Alleles; D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000096:biosynthesis / Q000235:genetics; D005800:Genes, Fungal; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013489:Suppression, Genetic
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV