title: [cGMP-dependent cyclic nucleotide phosphodiesterase from human lymphoid cells].
abstract: The kinetic properties of cyclic nucleotide phosphodiesterase isolated from the cytoplasmic fraction of lymphoblastoma QOS cells were studied. It was demonstrated that the enzyme can be activated in the presence of micromolar concentrations of cGMP. The kinetic properties of the enzyme are characterized by the nonlinear dependence of the cAMP hydrolysis rate on the substrate concentration. The curve becomes linear in the presence of cGMP. The molecular mass of phosphodiesterase as determined from gel filtration data is 80,0000 Da.
authors.forename: E V
authors.initials: EV
authors.lastname: Azhaeva
authors.forename: S E
authors.initials: SE
authors.lastname: Severin
authors.forename: A D
authors.initials: AD
authors.lastname: Kondrat'ev
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D002460:Cell Line; D006152:Cyclic GMP / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D008970:Molecular Weight
chemical_list: D015106:3',5'-Cyclic-GMP Phosphodiesterases; D006152:Cyclic GMP
vernacular_title: cGMP-zavisimaia fosfodiésteraza tsiklicheskikh nukleotidov limfoidnykh kletok cheloveka.