title: [Analysis of immunoregulatory peptides of the thymus using high performance liquid chromatography].
abstract: High performance liquid chromatography (gel filtration and chromatography in the reverse phase) was applied to analyse a composition of the heterogenous immunoregulating peptide preparation T-activine isolated from calf thymus. Gel chromatography at high pressure on the column TSK G2000 SW enabled to separate T-activine into 3 subfractions at a preparative scale. Physico-chemical properties and biological activity of these fractions were studied in vitro. Subfraction B with molecular mass of 1.0-3.5 kD and pI below 5.9 proved to be the most active.
authors.forename: M N
authors.initials: MN
authors.lastname: Korotkova
authors.forename: I V
authors.initials: IV
authors.lastname: Sanina
authors.forename: V Ia
authors.initials: VIa
authors.lastname: Arion
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D010455:Peptides / Q000032:analysis*; D013947:Thymosin / Q000032:analysis*; D013949:Thymus Extracts; D013950:Thymus Gland / Q000032:analysis*
chemical_list: D010455:Peptides; D013949:Thymus Extracts; D013947:Thymosin; C036191:T-activin
vernacular_title: Analiz immunoreguliatornykh peptidov timusa metodom vysokoéffektivnozhidkostnoĭ khromatografii.