title: Immunoperoxidase demonstration of the cellular composition of the normal and coeliac small bowel.
abstract: Immunohistological analysis of the cellular composition of the small intestinal mucosa in a group of untreated and treated coeliac patients and non-coeliac control subjects was performed using monoclonal antibodies and an immunoperoxidase technique. A characteristic cellular distribution was observed within the normal mucosa. The intraepithelial and lamina propria compartments were occupied mainly by T suppressor/cytotoxic and T helper/inducer cells respectively. Further subdivision of lamina propria T helper/inducer cells with the Leu 8 antibody revealed that these were of the Leu 3a+ Leu 8- phenotype. Macrophages, defined by the RFD7 antibody, were seen to occupy the same microenvironment as T helper/inducer cells. T cells expressing the T cell activation antigen defined by anti-Ta1 were found with the normal lamina propria, although few cells were identified by the anti-Tac antibody. HLA-Dr antigens were expressed by stellate cells within the lamina propria, and also by the epithelial cells of the villi, but not by normal crypt epithelial cells. In untreated coeliac patients the distribution of the various cell types was essentially unchanged, although the number of these cells was markedly increased, including those which expressed the Ta1 antigen. A significant deviation from normal in the expression of HLA-DR antigens was found in the coeliac small bowel: these antigens were expressed not only on the villous epithelial cells but also on the epithelial cells of the crypts. Immunohistological findings in the treated coeliac patients were intermediate between the normal and untreated coeliac groups, and were completely normal in those patients with complete histological resolution of their disease. These results suggest that coeliac disease is accompanied by an enhanced stimulation of the normal mucosal immune response and do not imply a primary pathogenic role for the immune system in this disease.
authors.affiliation: Department of Immunology, St James's Hospital, Dublin.
authors.forename: J
authors.initials: J
authors.lastname: Kelly
authors.forename: C
authors.initials: C
authors.lastname: O'Farrelly
authors.forename: C
authors.initials: C
authors.lastname: O'Mahony
authors.forename: D G
authors.initials: DG
authors.lastname: Weir
authors.forename: C
authors.initials: C
authors.lastname: Feighery
mesh_terms: D000954:Antigens, Surface / Q000032:analysis; D002446:Celiac Disease / Q000178:diet therapy / Q000276:immunology*; D005983:Glutens / Q000008:administration & dosage; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000276:immunology; D007421:Intestine, Small / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
chemical_list: D000954:Antigens, Surface; D006684:HLA-DR Antigens; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D005983:Glutens