Na-pump activity in rat kidney cortex cells and its relationship with the cell volume.
The present work was undertaken to evaluate whether changes in cell water content of rat kidney cortex cells can modulate the transport activity of the ouabain-insensitive Na pump as they modulate the ouabain-insensitive Na+-ATPase. It was found that there is a close relationship between the cell volume and activity of the Na pump, whereas Na,K-pump activity is not affected by variations in cell volume. When the cell water content is low, Na-pump activity (Na+ transport and Na+-ATPase activity) is minimal. Increases in cell water content produce a concomitant increase in Na-pump activity.
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Centro de Biofisica y Bioquímica, Instituto Venezolano de Investigáciones Científicas, Caracas.
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Matteo
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Perrone
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Marín
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D000251:Adenosine Triphosphatases / Q000502:physiology*; D000818:Animals; D001693:Biological Transport, Active; D027682:Cation Transport Proteins; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000502:physiology*; D008297:Male; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000502:physiology*; D014882:Water-Electrolyte Balance
D027682:Cation Transport Proteins; D012964:Sodium; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase; D000254:Sodium-Potassium-Exchanging ATPase