Fungal mycelia as a novel source of eicosapentaenoic acid. Activation of enzyme(s) involved in eicosapentaenoic acid production at low temperature.
Several filamentous fungi belonging to the genus Mortierella were found to produce large amounts of 5,8,11,14,17-cis-eicosapentaenoic acid (EPA) in their mycelia only when grown at low temperature (12 degrees C), i.e., not at physiological growth temperature (20-28 degrees C). The results of experiments with cell-free extracts suggested that this unique phenomenon is due to activation of enzyme(s) involved in EPA formation at low temperature. Mortierella alpina 1S-4 produced 0.3 g/l of EPA (27 mg/g dry mycelia). This high productivity show the practical significance of these novel EPA producers.
Shimizu
S
S
Department of Agricultural Chemistry, Kyoto University, Japan.
Shinmen
Y
Y
Kawashima
H
H
Akimoto
K
K
Yamada
H
H
D000255:Adenosine Triphosphate / Q000494:pharmacology; D003065:Coenzyme A / Q000494:pharmacology; D003080:Cold Temperature; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D004789:Enzyme Activation; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D009090:Mucorales / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides; D015636:Magnesium Chloride; D009249:NADP; D000255:Adenosine Triphosphate; D015118:Eicosapentaenoic Acid; D008274:Magnesium; D003065:Coenzyme A