Comparative study on O-linked oligosaccharides of glycoprotein D of herpes simplex virus types 1 and 2.
Glycoproteins D1 (gD1) and D2 (gD2) of herpes simplex virus type 1 and type 2, respectively, were purified from infected HEp-2 cells labelled with [3H]glucosamine for 14 h followed by a 3 h chase using HD1 monoclonal antibody linked to Sepharose. O-linked oligosaccharides were found to be present in both glycoproteins. The identification of N-acetyl [3H]galactosaminitol as the major labelled component in the oligosaccharides generated by mild alkaline borohydride treatment demonstrated that these chains have N-acetylgalactosamine at the reducing end. These oligosaccharides consist of mono- and disialylated species with a predominance of the latter species in gD1. Size analysis and radioactive amino sugar composition strongly suggest a structure in which the galactosyl-N-acetylgalactosamine core is substituted with one or two sialic acid residues. In terms of [3H]glucosamine-derived radioactivity, O-linked oligosaccharides are less represented than N-linked oligosaccharides. The O-linked oligosaccharide number determination showed that gD1 and gD2 carry two and three chains, respectively.
Serafini-Cessi
F
F
Sezione di Patologia generale, Università di Bologna, Italy.
Dall'Olio
F
F
Malagolini
N
N
Pereira
L
L
Campadelli-Fiume
G
G
D000116:Acetylgalactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis; D018139:Simplexvirus / Q000032:analysis*; D014759:Viral Envelope Proteins / Q000032:analysis*
D009844:Oligosaccharides; D012794:Sialic Acids; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; C040570:glycoprotein D-herpes simplex virus type 2; D019158:N-Acetylneuraminic Acid; D000116:Acetylgalactosamine; D005944:Glucosamine