Production of IL-1 alpha and IL-1 beta by clones of EBV transformed, human B cells.
An EBV transformed B-cell line was derived from peripheral blood cells from a single donor. B-cell clones were isolated from that line and analyzed for transcription of IL-1 genes, production of biologically detectable IL-1 and antigen presenting capabilities. One clone was found to secrete IL-1 activity into tissue culture medium, and to express the gene for IL-1 alpha. Three other clones were positive for cell surface IL-1 activity but no activity could be detected in culture supernatants. Those clones express the gene for IL-1 beta but not IL-1 alpha. All clones were approximately equal in their ability to present antigen to resting and primed T cells.
Acres
R B
RB
Immunex Corporation, Seattle, WA 98101.
Larsen
A
A
Gillis
S
S
Conlon
P J
PJ
D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D004854:Herpesvirus 4, Human; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology
D007375:Interleukin-1