Effect of glucose intake on human leucocyte 86Rb influx and [3H]-ouabain binding.
86Rb influx and [3H] ouabain binding by human leucocytes were measured in eight normal nonobese fasting subjects before and after a challenge with 75 g glucose orally. The mean ouabain-sensitive 86Rb influx increased significantly from 194 to 283 mmol/kg protein/h (P less than .01), and [3H]-ouabain binding increased from 236 to 403 fmol/mg protein. The mean plasma potassium concentration fell from 4.2 to 3.9 mmol/L (P less than .05). Following intravenous glucose infusion, the median 86Rb transport increased from 186 to 267 mmol/kg protein/h, while median plasma potassium concentration fell from 4.3 to 3.9 mmol/L. Therefore, glucose intake acutely increases Na-K ATPase units, stimulates potassium (Rb) transport, and causes a concomitant fall in plasma potassium concentrations. Nutritional intake is probably an important determinant of Na-K ATPase units and activity in the human leucocyte.
Turaihi
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Department of Chemical Pathology and Human Metabolism, Royal Free Hospital, London, United Kingdom.
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Dandona
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D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D012414:Rubidium Radioisotopes / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*; D014316:Tritium
D001786:Blood Glucose; D012414:Rubidium Radioisotopes; D014316:Tritium; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D011188:Potassium