Potassium monopersulfate and a water-soluble manganese porphyrin complex, [Mn(TMPyP)](OAc)5, as an efficient reagent for the oxidative cleavage of DNA.
Reported studies indicate that the association of potassium monopersulfate with [Mn(TMPyP)](OAc)5, a water-soluble manganese porphyrin complex, leads to an efficient reagent for the oxidative cleavage of DNA. Single-strand breaks (SSBs) are observed on double-stranded DNA at manganese porphyrin concentrations as low as 0.5 nM with a short incubation time of 1 min. The number of SSBs linearly varies with the concentration of the manganese complex, and potassium monopersulfate is at least 3 orders of magnitude more efficient as oxygen source than hydrogen peroxide. Cleavage efficiency is optimal in the pH range 7.5-9.0 for a NaCl concentration between 80 and 150 mM or for a MgCl2 concentration of 10 mM. At very low manganese porphyrin concentration and by increasing the incubation time a catalytic cleavage activity of the complex is evidenced: up to 5 SSBs per manganese porphyrin are observed. The high cleavage activity of the monopersulfate-manganese porphyrin system makes it a good candidate for DNA-footprinting experiments.
Bernadou
J
J
Laboratoire de Chimie de Coordination du CNRS, Toulouse, France.
Pratviel
G
G
Bennis
F
F
Girardet
M
M
Meunier
B
B
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA; D004277:DNA, Single-Stranded; D004587:Electrophoresis, Agar Gel; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride / Q000494:pharmacology; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D011188:Potassium; D017680:Potassium Compounds; D012965:Sodium Chloride / Q000494:pharmacology; D012995:Solubility; D013431:Sulfates; D013696:Temperature; D013997:Time Factors
D004277:DNA, Single-Stranded; D017680:Potassium Compounds; D013431:Sulfates; D015636:Magnesium Chloride; D012965:Sodium Chloride; C009007:potassium persulfate; D004247:DNA; D006861:Hydrogen Peroxide; D011188:Potassium; D010100:Oxygen