Trans-activation of class II (I-A alpha) gene by I-A beta gene transfection using bovine papilloma virus as a shuttle vector system.
We have engineered a bovine papilloma virus to carry the class II MHC (A beta d) gene. The recombinant plasmid was introduced into various mouse B cell hybridomas by co-transfecting with the neomycin-resistance gene. Several transfectants which received only the beta-chain gene of I-Ad, expressed I-Ad proteins on the cell surface. Their presence was determined by direct immunofluorescence and by Northern blot hybridization as well as RNA dot blot hybridization. These I-Ad molecules could induce IL-2 production in I-Ad restricted T cell hybridomas. When I-Ad molecules are expressed on the cell surface of the transfectants, they become fully active in inducing the I-Ad restricted T cell response. The original B cell hybridomas used for transfection possessed the alpha and beta chain genes of the I-Ad molecule but there are no transcripts of these genes in the cell. We suppose that many copies of the transfected beta-chain gene or its product may also induce production of cellular alpha-chain gene products and thereby induce expression of the I-Ad molecule on the cell surface.
Fukushima
J
J
Department of Bacteriology, Yokohama City University School of Medicine, Japan.
Tani
K
K
Atsumi
Y
Y
Hamajima
K
K
Kawamoto
S
S
Okuda
K
K
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D005111:Extrachromosomal Inheritance; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D005822:Genetic Vectors; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000235:genetics*; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007376:Interleukin-2 / Q000096:biosynthesis; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB; D027383:Papillomaviridae / Q000235:genetics*; D010641:Phenotype; D013601:T-Lymphocytes / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2