Tissue-specific DNase I-hypersensitive sites and hypomethylation in the chicken cardiac myosin light chain gene (L2-A).
As an approach to determine DNA elements involved in tissue-specific gene expression, the functional promoter of the cardiac myosin light chain gene (MLC 2-A) was identified. Chromatin structure of the MLC 2-A gene was examined for tissue specific DNase I-hypersensitive and hypomethylated sites. Heart specific DNase I-hypersensitive sites were detected in the promoter region of the gene as well as in flanking DNA sequences upstream and downstream of the gene. In expressing heart nuclei a region located about 1.5 kilobase pairs upstream of the transcription start site was found to contain both a hypersensitive and an undermethylated site. These structural chromatin changes of the MLC 2-A gene may be related to the expression of the gene.
Winter
B B
BB
Department of Toxicology, Medical School, University of Hamburg, Federal Republic of Germany.
Arnold
H H
HH
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005796:Genes; D008745:Methylation; D015879:Myosin Subfragments; D009218:Myosins / Q000235:genetics*; D009710:Nucleotide Mapping; D010446:Peptide Fragments / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013379:Substrate Specificity
D015879:Myosin Subfragments; D010446:Peptide Fragments; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D009218:Myosins