title: The genes of the Paracoccus denitrificans bc1 complex. Nucleotide sequence and homologies between bacterial and mitochondrial subunits.
abstract: The genes for the three subunits of the cytochrome bc1 complex from the bacterium Paracoccus denitrificans were identified by screening a gene library constructed in pBR 322 for expression using a cytochrome c1-specific antibody. These three genes coding for the FeS subunit, cytochrome b, and cytochrome c1 were located on contiguous sites on the genome in a presumed operon arrangement. The DNA-deduced amino acid sequence shows that all three subunits are homologous to corresponding polypeptides of the mitochondrial cytochrome bc1 complex. Cytochrome c1 of Paracoccus is much larger than its mitochondrial counterpart due to an extra 150 amino acids of unique, highly acidic composition; in addition, it is most likely synthesized as a precursor polypeptide.
authors.affiliation: Institute of Biochemistry, Medical University of Lübeck, West Germany.
authors.forename: B
authors.initials: B
authors.lastname: Kurowski
authors.forename: B
authors.initials: B
authors.lastname: Ludwig
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D014450:Electron Transport Complex III / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D009876:Operon; D010231:Paracoccus denitrificans / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004262:DNA Restriction Enzymes; D014450:Electron Transport Complex III