title: Inhibition of phagocytosis and interleukin-1 production in pulmonary macrophages from rats with sialodacryoadenitis virus infection.
abstract: To test whether or not sialodacryoadenitis virus (SDAV) infection in rats affects pulmonary macrophage function, we intranasally inoculated pathogen-free F344 rats with SDAV and collected alveolar and interstitial macrophages 5 d later. We assessed Fc receptor-mediated attachment and phagocytosis by phase-contrast microscopic examination of monolayers of alveolar and interstitial macrophages incubated with zymosan, nonopsonized sheep erythrocytes, or erythrocytes opsonized with rabbit antisheep-erythrocyte IgG. Alveolar macrophages from virus-infected rats had significantly (P less than or equal to .05) lower indices of attachment and phagocytosis of opsonized erythrocytes than control macrophages, but there was no difference in attachment of zymosan particles. Interstitial macrophages were not affected. Alveolar macrophages from SDAV-infected rats produced significantly less interleukin-1 than those from control rats, as assessed by testing supernatants from lipopolysaccharide-stimulated macrophage cultures for induction of mouse thymocytes to take up tritiated thymidine. Effects of SDAV infection on lung macrophages could increase host susceptibility to other pathogens or complicate studies of respiratory tract immunity.
authors.affiliation: Department of Comparative Medicine, University of Alabama, Birmingham 35294.
authors.forename: K R
authors.initials: KR
authors.lastname: Boschert
authors.forename: T R
authors.initials: TR
authors.lastname: Schoeb
authors.forename: D B
authors.initials: DB
authors.lastname: Chandler
authors.forename: D L
authors.initials: DL
authors.lastname: Dillehay
mesh_terms: D000208:Acute Disease; D000818:Animals; D002448:Cell Adhesion; D003333:Coronaviridae Infections / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D005260:Female; D007113:Immunity, Innate; D007165:Immunosuppression Therapy; D007375:Interleukin-1 / Q000096:biosynthesis*; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010587:Phagocytosis; D011650:Pulmonary Alveoli / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D011961:Receptors, Fc / Q000502:physiology
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D011961:Receptors, Fc