Stabilization of DNA-polycation complex by lithium perchlorate.
The polycation forms a complex with DNA. The complex was stabilized to thermal denaturation by millimolar concentrations of LiClO4, in the range of 0.1-0.10 M LiClO4. No hyperchromicity was observed in the thermal profiles, and instead, hyperchromicity was observed. The complex showed no significant extrinsic CD band, but showed LiClO4-dependent alterations in the DNA intrinsic bands. It was found that Li+ was primarily responsible for the stabilization of the complex. It is proposed that Li+ stabilization arises through ternary complex formation and that the chaotropic ClO4- catalyzes conversion of less stable DNA-polycation complex into a more stable form.
Mulimani
V H
VH
Department of Chemistry, Gulbarga University, India.
Day
R A
RA
D002412:Cations / Q000378:metabolism*; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D008094:Lithium / Q000494:pharmacology*; D018020:Lithium Compounds; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D010472:Perchlorates / Q000494:pharmacology*; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D002412:Cations; D004247:DNA; D008094:Lithium; D018020:Lithium Compounds; D010472:Perchlorates; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; C054684:lithium perchlorate