[Effects of complex of 3,6-di-(dimethylamino)-dibenzopyriodonium with praseodymium dicitrate on the syntheses of DNA, RNA, protein, nucleoprotein and ATP of leukemia L 7712 cells in mice].
The incorporations of [3H] thymidine, [3H]uridine and [3H]leucine into DNA, RNA and protein synthesis in leukemia 7712 cells were inhibited by the complex of 3,6-di-(dimethylamino)-dibenzopyriodonium with praseodymium (Pr, rare earth element) dicitrite 34 micrograms/ml for 3-24 h. The degree of inhibition increased in proportion to the incubation time. After being treated with [C17H20N2I]3[Pr(C6H5O7)2] 34 micrograms/ml for 3, 6, 12 and 24 h, the incorporation of [32P]Na2HPO4 into the nucleoprotein of leukemia 7712 cells was inhibited by 49, 57, 65 and 85%, while those into ATP were inhibited by 43, 59, 65 and 83%, respectively. The ID50 of [C17H20N2I]3[Pr(C6H5O7)2] on DNA synthesis in leukemia 7712 cells at 24 h was 22 micrograms/ml. After the complex was removed from the medium entirely, the rate of DNA synthesis decreased with time over 3-12 h. This result indicated that the inhibition mechanism was likely due to damage to the DNA template.
Liu
L S
LS
Wang
P
P
Wang
S Z
SZ
Zheng
R L
RL
Zhang
L D
LD
Chen
S Y
SY
Wang
C H
CH
Hou
Z J
ZJ
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000187:drug effects; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009698:Nucleoproteins / Q000096:biosynthesis; D009861:Onium Compounds / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000494:pharmacology*; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000187:drug effects
D001580:Benzopyrenes; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D009861:Onium Compounds; D009942:Organometallic Compounds; D012334:RNA, Neoplasm; C061550:3,6-di(dimethylamino)dibenzopyriodonium praseodymium dicitrate complex; D000255:Adenosine Triphosphate