title: Nucleotide sequence and deduced amino acid sequence of a coleopteran-active delta-endotoxin gene from Bacillus thuringiensis subsp. san diego.
abstract: A gene from Bacillus thuringiensis subsp. san diego that is responsible for a delta-endotoxin active against Colorado potato beetle and some other Coleoptera was sequenced and shown to have surprising regional homology with both lepidopteran and dipteran active delta-endotoxins from other strains of B. thuringiensis. Unlike the lepidopteran active toxins from B. thuringiensis subsp. kurstaki that exist as approx. 130-kDa protoxins and form bipyramidal crystalline inclusions, the coleopteran toxic protein forms a square-shaped crystal composed of an approx. 65-kDa protein. Comparisons of the gene sequences encoding the active portions of these protoxins indicate conservation of N-terminal hydrophilic and hydrophobic regions, and suggest a distant ancestral origin for these insecticidal proteins.
authors.affiliation: Mycogen Corporation, San Diego, CA 92121.
authors.forename: C
authors.initials: C
authors.lastname: Herrnstadt
authors.forename: T E
authors.initials: TE
authors.lastname: Gilroy
authors.forename: D A
authors.initials: DA
authors.lastname: Sobieski
authors.forename: B D
authors.initials: BD
authors.lastname: Bennett
authors.forename: F H
authors.initials: FH
authors.lastname: Gaertner
mesh_terms: D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004731:Endotoxins / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D007306:Insecticides; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010944:Plants; D010957:Plasmids
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D004262:DNA Restriction Enzymes