A recombinant murine retrovirus expressing v-rel is cytopathic.
A murine retrovirus that expresses the avian v-rel oncogene was constructed. NIH 3T3 cells transfected with this construct expressed v-rel-specific RNA and a 59-kDa protein serologically identical to avian v-rel. The protein expressed from the recombinant retrovirus retained the associated protein kinase activity observed in avian systems. While the detection of v-rel RNA sequences in infected cells verified the infectivity of the retrovirus, the retrovirus did not transform either murine fibroblasts or bone marrow cells. Rather, a cytopathic effect was observed in murine fibroblasts and a pre-B lymphoid cell line that were infected with the murine retrovirus. Growth curves of these infected cells revealed cell death or diminished growth rate in all cases.
Schwartz
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Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
Witte
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D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001854:Bone Marrow Cells; D002460:Cell Line; D002472:Cell Transformation, Viral; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts / Q000382:microbiology; D005814:Genes, Viral; D051379:Mice; D008807:Mice, Inbred BALB C; D008979:Moloney murine leukemia virus / Q000235:genetics*; D020572:Oncogene Proteins v-rel; D011994:Recombinant Proteins / Q000276:immunology; D001356:Reticuloendotheliosis virus / Q000235:genetics* / Q000276:immunology; D012190:Retroviridae / Q000235:genetics*; D012191:Retroviridae Proteins / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D020572:Oncogene Proteins v-rel; D011994:Recombinant Proteins; D012191:Retroviridae Proteins