HLA class I antigens on normal and leukemic cells (quantitative analysis).
A monomorphic anti-Class I monoclonal antibody, ST01, found in our laboratory, was used to quantify Class I antigens on normal and leukemic cells, using a "CELISA" technique. Saturation graphs were used to compare the quantity of Class I antigens on normal PBL (25 cases) with that on the following types of leukemic cells: a) common acute lymphoblastic leukemias (cALL) (11 cases), b) mature B lymphocytic proliferations (16 cases), c) T hemopathies (9 cases), d) non-lymphoid leukemias (9 cases). In most cases the quantity of HLA Class I antigens was greatly reduced. No correlation was found between the quantitative expression of Class I antigens and the stage of maturation in each cell type, nor was any correlation found between the quantitative expression of Class I antigens on the leukemic cells and the proliferation of leukemic cells in the peripheral blood.
Urlacher
A
A
Institut d'Hématologie, Centre de Transfusion Sanguine, Strasbourg, France.
Falkenrodt
A
A
Tongio
M M
MM
Mayer
S
S
D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006680:HLA Antigens / Q000032:analysis*; D004854:Herpesvirus 4, Human; D006801:Humans; D007938:Leukemia / Q000145:classification / Q000276:immunology*; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D006680:HLA Antigens; D010835:Phytohemagglutinins