[Synthesis of polypeptides of various length in isolated mitochondria of rat liver].
The [35S]methionine labeled mitochondrial translation products were separated by SDS-PAGE electrophoresis in the presence of 8 M urea. The time of the major polypeptide synthesis was determined after fluorography and scanning. It was found that the changes in the time of polypeptide synthesis are not correlated with their lengths. These data are consistent with the hypothesis on the unsteady rate of protein synthesis with a sharp deceleration (pause) of translation during the reading of middle portions of mRNA.
Kreĭnin
M O
MO
Rabinovich
Ia M
IaM
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
D012333:RNA, Messenger
Sintez polipeptidov razlichnoĭ deliny v izolirovannykh mitokhondriiakh pecheni krysy.