Changes in lipoxygenase products from synovial fluid in carrageenan induced arthritis in dogs.
A non-suppurative chronic arthritis was induced in the juvenile dog knee by intra-articular instillations with Carrageenan. Lipoxygenase products of arachidonic acid were separated from synovial fluid by reversed-phase high-performance liquid chromatography (RP-HPLC). After ten weeks we observed an accumulation of leukotriene B4 (LTB4) in synovial fluid in five out of six experimental knees (0.94 to 5.5 ng/ml), as judged by integrated optical density. Biological activity of LTB4 was confirmed by chemokinesis. LTB4 was not detected in control knees. The 15-lipoxygenase products, 15-hydroxyeicosatetraenoic acid (15-HETE) and 13-hydroxy-9,11-octadecadienoic acid (13-HODD), being inhibitors of 5-lipoxygenase, were found in relatively high levels in the control knees compared to the arthritic knees. The results denote LTB4 as a pro-inflammatory local mediator during carrageenan-induced arthritis; possibly, the decreased levels of 15-HETE and 13-HODD in the arthritic knees may have a regulatory function, thus facilitating LTB4 generation.
Herlin
T
T
Department of Pediatrics, Aarhus Kommunehospital, Denmark.
Fogh
K
K
Ewald
H
H
Hansen
E S
ES
Knudsen
V E
VE
Holm
I
I
Kragballe
K
K
Bunger
C
C
D000818:Animals; D001168:Arthritis / Q000201:enzymology* / Q000276:immunology; D002351:Carrageenan; D002634:Chemotaxis, Leukocyte; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004285:Dogs; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D007975:Leukotriene B4 / Q000276:immunology / Q000378:metabolism*; D008041:Linoleic Acids / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D013582:Synovial Fluid / Q000201:enzymology* / Q000276:immunology
D006893:Hydroxyeicosatetraenoic Acids; D008041:Linoleic Acids; D007975:Leukotriene B4; C024348:13-hydroxy-9,11-octadecadienoic acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D002351:Carrageenan; D008084:Lipoxygenase