Characterization of Pi-repressible enzymes secreted in culture media by Neurospora crassa wild-type cells and null-type mutants.
In wild-type mycelial cultures of Neurospora crassa under Pi-limited conditions, alkaline phosphatase, cyclic phosphodiesterases I, II, III, and IV, 5'-nucleotidase, acid and alkaline nucleases, RNase N1, and a newly detected endonuclease were secreted into the culture media. These enzymes were either not produced or were produced in very reduced levels in mutants nuc-1, -2, -3, -4, -5, -6, and -7 and cpd-4. The proteins were examined by polyacrylamide gel electrophoresis in a manner which allowed the identification of each of them.
Furukawa
K
K
National Institute for Basic Biology, Okazaki, Japan.
Hasunuma
K
K
Shinohara
Y
Y
D015720:5'-Nucleotidase; D000469:Alkaline Phosphatase / Q000032:analysis / Q000096:biosynthesis; D002850:Chromatography, Gel; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000032:analysis / Q000096:biosynthesis; D004794:Enzyme Repression; D005656:Fungal Proteins / Q000032:analysis*; D009154:Mutation; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics; D009708:Nucleotidases / Q000032:analysis / Q000096:biosynthesis; D010710:Phosphates / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000032:analysis / Q000096:biosynthesis; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000096:biosynthesis*; D012260:Ribonucleases / Q000032:analysis / Q000096:biosynthesis
D003470:Culture Media; D005656:Fungal Proteins; D010710:Phosphates; D004720:Endonucleases; D012260:Ribonucleases; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases