Inhibition of herpes simplex virus DNA replication by ara-tubercidin.
Preliminary studies of the biochemical basis for the antiviral activity of the pyrrolo[2,3-d]pyrimidine nucleoside ara-tubercidin were conducted. Herpes simplex virus DNA synthesis was 3-fold more sensitive to inhibition by ara-tubercidin than was cellular DNA synthesis. Partially purified herpes DNA polymerases were more sensitive to inhibition by ara-tubercidin 5'-triphosphate than were cellular polymerases alpha and beta. Inhibition of viral DNA polymerase was competitive with dATP and noncompetitive with dTTP. The results suggest that the viral DNA polymerase plays a significant role in the antiviral activity of ara-tubercidin.
Turk
S R
SR
Department of Oral Biology, School of Dentistry, University of Michigan, Ann Arbor 48109.
Cook
P D
PD
Reinke
C M
CM
Drach
J C
JC
D000998:Antiviral Agents / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D007624:KB Cells; D007700:Kinetics; D019384:Nucleic Acid Synthesis Inhibitors; D018139:Simplexvirus / Q000187:drug effects* / Q000235:genetics
D000998:Antiviral Agents; D019384:Nucleic Acid Synthesis Inhibitors