Influence of a cecal volume-reducing intestinal microflora on the excretion and entero-hepatic circulation of steroids and bile acids.
From mouse fecal material we have isolated four strictly anaerobic bacteria which, when associated with germfree mice or rats, reduced the cecal volume by 80 and 60%, respectively. This cecal volume-reducing flora did not metabolize estrone-3-sulfate, taurolithocholate-3-sulfate or taurolithocholate but gnotobiotic rats associated with this particular flora (CRF-rats) excreted these compounds faster in feces plus urine than did germfree rats. The time needed for 50% excretion (t1/2) of orally administered estrone-3-sulfate was 32 h in germfree rats versus 13 h in CRF rats; for intraperitoneally injected taurolithocholate-3-sulfate the t1/2 was 63 h in germfree versus 17 h in CRF rats and for taurolithocholate the t1/2 was 199 h in germfree and 96 h in CRF rats. Association of germfree rats with the cecal volume-reducing flora did not change the cecal absorption rate of estrone-3-sulfate, but shortened the 50% small intestinal transit time of [14C]PEG from 10 to 3 h; a value also found in conventional rats. These results stress the important influence of the intestinal microflora on the absorption and excretion of steroids via its effect on the physiology of the whole intestinal tract and point to the deficiencies inherent to the use of germfree animals in excretion studies.
Van Eldere
J
J
Rega Institute, Catholic University of Leuven, Belgium.
Robben
J
J
Caenepeel
P
P
Eyssen
H
H
D000818:Animals; D002432:Cecum / Q000378:metabolism / Q000382:microbiology* / Q000502:physiology; D004764:Enterohepatic Circulation; D004966:Estrogens, Conjugated (USP) / Q000493:pharmacokinetics / Q000652:urine; D004970:Estrone / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000652:urine; D005243:Feces / Q000032:analysis / Q000382:microbiology; D005260:Female; D005772:Gastrointestinal Transit; D007408:Intestinal Absorption; D008095:Lithocholic Acid / Q000031:analogs & derivatives*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013658:Taurolithocholic Acid / Q000493:pharmacokinetics* / Q000652:urine
D004966:Estrogens, Conjugated (USP); D004970:Estrone; D013658:Taurolithocholic Acid; D008095:Lithocholic Acid; C017296:estrone sulfate