The isolation and purification of pre-S2 containing hepatitis B virus surface antigen by chemical affinity chromatography.
A simple, rapid, and efficient method was developed to isolate and purify pre-S2 containing HBsAgs from the plasma of a single chronic carrier of HBsAg (adw) by ammonium sulfate fractionation, hydroxyapatite column chromatography, and polymerized human serum albumin-affinity column chromatography. About 500 micrograms of pre-S2 containing HBsAg was obtained from 140 mL of plasma containing 4,200 micrograms of HBsAg. Two purified pre-S2 containing HBsAgs were analyzed by SDS-polyacrylamide gel electrophoresis and their molecular weights were determined to be 31,000 and 68,000 respectively. No significant amount of HBsAg or its derivative was detected in the final product.
Lin
J Y
JY
Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, R.O.C.
Hsieh
Y S
YS
Chu
S C
SC
D002845:Chromatography / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D006801:Humans; D006882:Hydroxyapatites; D008970:Molecular Weight; D011498:Protein Precursors / Q000302:isolation & purification*; D012709:Serum Albumin
D006514:Hepatitis B Surface Antigens; D006882:Hydroxyapatites; D011498:Protein Precursors; D012709:Serum Albumin; C050807:presurface protein 2, hepatitis B surface antigen; D017886:Durapatite