Identification and characterization of a novel membrane activation antigen with wide cellular distribution.
A monoclonal antibody, 7F7, raised against the Raji B lymphoblastoid cell line reacted with about 35% of peripheral blood B cells, germinal center B cells, follicular dendritic cells and some vascular endothelial cells. Although not found on resting T cells this antigen was strongly expressed on CD4+ and CD8+ T cells activated with phytohemagglutinin, pokeweed mitogen and anti-T3, and its expression on B cells was enhanced after stimulation with pokeweed mitogen. It is strongly expressed 24 h after stimulation with phytohemagglutinin and its expression is reduced but not eliminated by cyclosporin A treatment. The molecule defined by antibody 7F7 is found on some, but not all, B cell lines, on an HTLV-I-transformed T cell line and on the promyelocytic cell line U937. Immunoprecipitation from externally and metabolically labeled Raji cells revealed a single-chain molecule of 85 kDa.
Schulz
T F
TF
Institute for Hygiene, Innsbruck, Austria.
Mitterer
M
M
Vogetseder
W
W
Böck
G
G
Myones
B L
BL
Dierich
M P
MP
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001402:B-Lymphocytes / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D003524:Cyclosporins / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000032:analysis* / Q000276:immunology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003524:Cyclosporins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7