Binding of BAL 31 RNA polymerase to PM2 DNA as determined by electron microscopy and protection against restriction endonuclease cleavage.
Specific binding sites of BAL 31 RNA polymerase on PM2 DNA have been mapped by protection against HincII and HindIII cleavage and by observation of enzyme-DNA complexes by electron microscopy. Nine specific binding sites were observed at map units 0.19, 0.20, 0.28, 0.54, 0.63, 0.65, 0.71, 0.72, and 0.75 by the first method. All these sites were confirmed by electron microscopy which, in addition, revealed another site at 0.05 map unit. Published nucleotide sequences of the region surrounding sites at 0.71 and 0.75 map units show the presence of consensus sequences for procaryotic promoters.
Bull
P
P
Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Santiago.
Susaeta
M
M
González
B
B
Yudelevich
A
A
D001435:Bacteriophages / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004278:DNA, Superhelical / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism* / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D008854:Microscopy, Electron; D011401:Promoter Regions, Genetic
D004278:DNA, Superhelical; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases