Isolation of high-molecular-weight DNA from eukaryotic cells by formamide treatment and dialysis.
A new method for the isolation of high-molecular-weight DNA from eukaryotic cells is presented. It is based on formamide treatment and extensive dialysis of cellular proteinase K digests. This procedure consists mainly of a series of incubations allowing simultaneous preparation of several DNAs which can then be restricted, ligated, hybridized, and cloned.
Kupiec
J J
JJ
UER d'Hématologie, LOI CNRS, Hôpital Saint-Louis, Paris, France.
Giron
M L
ML
Vilette
D
D
Jeltsch
J M
JM
Emanoil-Ravier
R
R
D000818:Animals; D002460:Cell Line; D002477:Cells / Q000032:analysis*; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K; D005057:Eukaryotic Cells / Q000032:analysis*; D005559:Formamides; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D009369:Neoplasms / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012697:Serine Endopeptidases
D005559:Formamides; C031066:formamide; D004247:DNA; D004262:DNA Restriction Enzymes; D012697:Serine Endopeptidases; D019286:Endopeptidase K