Competitive ion binding to low density lipoproteins: an electron spin resonance study.
The electron spin resonance (ESR) technique was used to evaluate binding constants for Ca(II) and Mg(II) in interaction with low density lipoprotein (LDL). The Ca(II) or Mg(II) ions competed with the paramagnetic Mn(II) ions for the same binding sites of two different classes on the LDL surface. For each ion competing with Mn(II), the solutions of eight non-linear competition equations were fit to the experimental titration curves, with two adjustable parameters, the two binding constants. The derived "intrinsic" values (the values corrected for the electrolyte-induced change of the surface potential) for "strong" binding sites for Ca(II) (170 +/- 85 M-1) and Mg(II) (60 +/- 30 M-1) differ significantly from the respective value for Mn(II) (760 M-1). The values for the "weak" binding sites (18 M-1, 15 M-1 and 10 M-1 for Mn(II), Ca(II) and Mg(II), respectively are in the range of the binding constants for these ions in interaction with model membranes.
Pifat
G
G
Rudjer Bosković Institute, University of Zagreb, Yugoslavia.
Udovicić
L
L
Brnjas-Kraljević
J
J
Jürgens
G
G
Holasek
A
A
Herak
J N
JN
D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D011485:Protein Binding
D008077:Lipoproteins, LDL; D008345:Manganese; D008274:Magnesium; D002118:Calcium