Reductive methods for isotopic labeling of antibiotics.
Methods for the reductive methylation of the amino groups of eight different antibiotics using 3HCOH or H14COH are presented. The reductive labeling of an additional seven antibiotics by NaB3H4 is also described. The specific activity of the methyl-labeled drugs was determined by a phosphocellulose paper binding assay. Two quantitative assays for these compounds based on the reactivity of the antibiotic amino groups with fluorescamine and of the aldehyde and ketone groups with 2,4-dinitrophenylhydrazine are also presented. Data on the cellular uptake and ribosome binding of these labeled compounds are also presented.
Champney
W S
WS
Department of Biochemistry, College of Medicine, East Tennessee State University, Johnson City 37614.
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002250:Carbon Radioisotopes; D002482:Cellulose / Q000031:analogs & derivatives; D005450:Fluorescamine; D007553:Isotope Labeling / Q000379:methods*; D010084:Oxidation-Reduction; D012545:Schiff Bases; D014316:Tritium
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D012545:Schiff Bases; D014316:Tritium; D005450:Fluorescamine; D002482:Cellulose; C008881:phosphocellulose