[A microtitration and neutralization test for infectious laryngotracheitis virus. Their reproducibility and the effect of various tests conditions].
The reproducibility of micro-assays for ILTV was assessed. The standard deviation of the virus assay in three successive determinations was 0.4, 0.4 and 0.3 log TCID50. The micro-neutralisation test produced standard deviations of 0.6, 0.8, 0.5, 1.2 and 1.1 log2 in various experiments. Preincubation for 24 hours at 39 degrees C, rather than for sixty minutes at 22 degrees C, which was suggested by Bitsch to enhance sensitivity, resulted in antibody titres which were approximately 1 log2 higher, though the specificity of the test declined markedly. The dose-response relationship between virus and antibody titre was curvilinear, the steepest part of the slope being in the lower range. Thermal inactivation and freezing and thawing resulted in a significant reduction of serum titres (P less than 0.01). The following method is suggested as a practical standardised procedure: freezing of sera prior to processing: omission of thermal inactivation and preincubation for sixty minutes at 22 degrees C.
Hilbink
F W
FW
Centraal Diergeneeskundig Instituut, Lelystad.
van Roozelaar
D J
DJ
D000818:Animals; D002642:Chick Embryo; D006564:Herpesviridae / Q000276:immunology*; D001352:Herpesvirus 1, Gallid / Q000276:immunology*; D007158:Immunologic Techniques; D009500:Neutralization Tests; D013696:Temperature; D013997:Time Factors
Een microtitratie en -neutralisatietest voor infectieuze Laryngotracheïtisvirus. Hun reproduceerbaarheid en de invloed van verschillende testvoorwaarden.