The mitochondrial S13 ribosomal protein gene is silent in wheat embryos and seedlings.
The sequence of a wheat mitochondrial reading frame encoding a protein homologous to the E. coli S13 small subunit ribosomal protein has been determined. The gene is located immediately downstream of a 1.4 kb recombinationally-active repeat element that contains the ATPase subunit 6 gene. The coding regions of the two genes are separated by only 153 bp, the shortest distance yet observed between protein-coding genes in plant mitochondria. However, their transcript profiles differ markedly. The ATPase 6 gene displays a single, prominent mRNA of approximately 1.4 kb, whereas the S13 gene shows no stable transcript as judged by Northern blot analysis of wheat mitochondrial RNA isolated from different developmental stages. A short segment of the 26S rRNA gene is located downstream of the S13 gene and its presence illustrates the frequent DNA duplication/rearrangements found in wheat mitochondria.
Bonen
L
L
Department of Biology, University of Ottawa, Canada.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics*
D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D004262:DNA Restriction Enzymes