title: [Molecular organization of the N-terminal region of delta-endotoxin of Bacillus thuringiensis subspecies alesti].
abstract: The tryptic peptide sequences of the N-terminal domain ("true toxin") of delta-endotoxin of Bac. thuringiensis subspecies alesti carrying 282 amino acid residues were determined. A comparison of these sequences with the primary structures of delta-endotoxin of subspecies kurstaki (K-1, K-73) determined by an analysis of corresponding structural genes revealed a conservative region of "true toxin" (residues 29-346) and a hypervariable region (residues 347-617) carrying multiple (not less than 50%) substituents of amino acid residues. It is essential that the amino acid substituents in the variable region are distributed unevenly, being grouped into several highly variable sites carrying 7 to 31 residues. Besides, tryptic peptides of subspecies alesti delta-endotoxin were found to contain peptides having no homologs in the structures of subspecies kurstaki delta-endotoxins. It seems probable that such an uneven distribution of amino acid substituents in the structures of delta-endotoxins of subspecies alesti and kurstaki reflects the functional differences in the two halves of the N-terminal domain ("true toxin"), one of which (i. e., conservative) may be responsible for the toxic effect, while the other one (i. e., variable) seems to participate in toxin interactions with the appropriate receptors of larvae gut.
authors.forename: S A
authors.initials: SA
authors.lastname: Tiurin
authors.forename: G G
authors.initials: GG
authors.lastname: Chestukhina
authors.forename: A L
authors.initials: AL
authors.lastname: Osterman
authors.forename: O M
authors.initials: OM
authors.lastname: Khodova
authors.forename: E A
authors.initials: EA
authors.lastname: Timokhina
mesh_terms: D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000032:analysis* / Q000145:classification; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis; D001427:Bacterial Toxins; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004731:Endotoxins / Q000032:analysis* / Q000302:isolation & purification; D006460:Hemolysin Proteins; D006868:Hydrolysis; D010447:Peptide Hydrolases
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D010447:Peptide Hydrolases
vernacular_title: Molekuliarnaia organizatsiia N-kontsevogo domena delta-éndotoksina podvida alesti Bac. thuringiensis.