LiCl treatment releases a nickase implicated in genetic transformation of Streptococcus pneumoniae.
When cells competent for genetic transformation of Streptococcus pneumoniae which could bind and enable entry of extracellular DNA molecules were treated with LiCl, they released a nickase that introduced nicks into a double-stranded DNA in the presence of EDTA. The nickase was specific for competent cells and coupled with DNA-binding activity. Furthermore, when noncompetent cells were treated with LiCl, they released the putative receptors for the competence activator.
Fujii
T
T
Department of Applied Biological Science, Science University of Tokyo, Chiba, Japan.
Naka
D
D
Toyoda
N
N
Seto
H
H
D002712:Chlorides / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D003851:Deoxyribonucleases / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology*; D018021:Lithium Chloride; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic / Q000187:drug effects*
D002712:Chlorides; D004268:DNA-Binding Proteins; D008094:Lithium; D003851:Deoxyribonucleases; D018021:Lithium Chloride