Developmental competency in spinal nerve fiber extension on a reconstituted basement-membrane-like matrix.
Nerve fibers readily extend from larval frog spinal cord explants when cultured on a commercially available reconstituted basement-membrane-like matrix derived from the EHS mouse sarcoma. This superior, naturally derived substratum enables the in vitro development of complex neuritic arrays with long survival times in a defined medium, thus permitting the detailed analysis of spontaneous and target-mediated neuritogenesis.
Liebig
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Institute for the Study of Developmental Disabilities, University of Illinois, Chicago 60680.
Pollack
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ED
D000818:Animals; D001485:Basement Membrane / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005109:Extracellular Matrix / Q000502:physiology*; D007814:Larva / Q000502:physiology; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D011894:Rana pipiens; D013127:Spinal Nerves / Q000166:cytology / Q000187:drug effects / Q000196:embryology*
D003470:Culture Media