Abnormal neutrophils in acute myeloid leukemia and myelodysplastic syndrome.
Neutrophils and band forms from patients with acute myeloid leukemia and myelodysplastic syndrome were stained for the presence of myeloperoxidase using a cytochemical method (diaminobenzidine/hydrogen peroxide) and the alkaline phosphatase--anti-alkaline phosphatase immunocytochemical procedure (using monoclonal anti-myeloperoxidase). Neutrophils and bands were also stained for elastase and lactoferrin using monoclonal and polyclonal antibodies, respectively. Subpopulations of neutrophils and bands from cases of acute myeloid leukemia and myelodysplasia exhibited a qualitative and/or quantitative deficiency in myeloperoxidase. In addition, a quantitative decrease in elastase and/or lactoferrin staining was detected. Thus, neutrophils and bands from patients with acute myeloid leukemia and myelodysplastic syndrome have a defect in one or more of the constituents of primary and/or secondary granules. These defects are consistent with the view that abnormal neutrophils and bands are derived from a malignant clone of myeloid precursor cells.
Davey
F R
FR
Department of Pathology, State University of New York Health Science Center, Syracus 13210.
Erber
W N
WN
Gatter
K C
KC
Mason
D Y
DY
D003594:Cytoplasmic Granules / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D007781:Lactoferrin / Q000097:blood; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology / Q000473:pathology*; D009190:Myelodysplastic Syndromes / Q000201:enzymology / Q000473:pathology*; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology*; D010196:Pancreatic Elastase / Q000097:blood; D009195:Peroxidase / Q000097:blood
D009195:Peroxidase; D007781:Lactoferrin; D010196:Pancreatic Elastase