Tissue distribution of an endogenous ligand to the Na, K ATPase molecule.
A variety of evidence indicates the presence of a circulating ligand to the Na, K ATPase molecule that is involved in the regulation of extracellular sodium metabolism. To examine the potential role of endogenous ligands to the Na, K ATPase molecule in the regulation of intracellular sodium metabolism, the tissue distribution of digitalis-like activity was quantitated in several brain regions and peripheral organs. The digitalis-like activity of desalted and delipidated extracts of tissue was widely distributed and produced a displacement of tritiated ouabain that was parallel to the displacement produced by cold ouabain. These results suggest that tissue contains an endogenous ligand to the Na, K ATPase molecule and that this ligand may regulate intracellular sodium metabolism in an autocoid-like manner.
Rauch
A L
AL
Department of Medicine, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103.
Buckalew
V M
VM
D000818:Animals; D001667:Binding, Competitive; D001798:Blood Proteins / Q000378:metabolism* / Q000502:physiology; D001923:Brain Chemistry; D002298:Cardenolides; D004077:Digoxin; D007424:Intracellular Fluid / Q000378:metabolism; D008297:Male; D010042:Ouabain / Q000378:metabolism; D011487:Protein Conformation; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug / Q000187:drug effects; D012503:Saponins; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism / Q000502:physiology*; D014018:Tissue Distribution
D001798:Blood Proteins; D002298:Cardenolides; D011955:Receptors, Drug; D012503:Saponins; C028996:cardiac glycoside receptors; C034504:digoxin-like factors; D010042:Ouabain; D004077:Digoxin; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase