Ectoenzymes of the kidney microvillar membrane. Differential solubilization by detergents can predict a glycosyl-phosphatidylinositol membrane anchor.
The pattern of solubilization of nine kidney microvillar ectoenzymes by a range of detergents distinguished two classes of membrane proteins: those released from the membrane by bacterial phosphatidylinositol-specific phospholipase C and those not so released. The latter group of transmembrane proteins were solubilized efficiently (greater than 80%) by all the detergents examined. In contrast, proteins released by phosphatidylinositol-specific phospholipase C were solubilized effectively only by octyl glucoside, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulphonate and sodium deoxycholate. Octyl glucoside solubilized the amphipathic forms of the ectoenzymes examined, suggesting that this may be a useful detergent in the purification of glycosyl-phosphatidylinositol-anchored ectoenzymes.
Hooper
N M
NM
Department of Biochemistry, University of Leeds, U.K.
Turner
A J
AJ
D000818:Animals; D003902:Detergents / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology; D006017:Glycolipids / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols; D006867:Hydrolases / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology*; D008565:Membrane Proteins / Q000378:metabolism*; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology; D017830:Octoxynol; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D043264:Phosphodiesterase I; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012995:Solubility; D013501:Surface-Active Agents / Q000494:pharmacology*; D013552:Swine
D003902:Detergents; D005960:Glucosides; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D013501:Surface-Active Agents; C018619:octyl-beta-D-glucoside; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D006867:Hydrolases; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase