title: In situ localization of human papilloma virus type 16 DNA in a metastasis of an endocervical adenocarcinoma.
abstract: Metastatic tissue from an endocervical adenocarcinoma was examined for human papilloma virus (HPV) DNA sequences. Southern blot analysis with 32P-labeled viral probes revealed the presence of about 150 copies of HPV-16 DNA per cellular genome. DNA in situ hybridization of frozen sections with biotinylated probes showed that HPV-16 DNA was exclusively detectable in the nuclei of the malignant epithelial cells and not in the stroma.
authors.affiliation: Department of Pathology, University of Amsterdam, The Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: Walboomers
authors.forename: H E
authors.initials: HE
authors.lastname: Fokke
authors.forename: M
authors.initials: M
authors.lastname: Polak
authors.forename: H
authors.initials: H
authors.lastname: Volkers
authors.forename: H J
authors.initials: HJ
authors.lastname: Houthoff
authors.forename: J
authors.initials: J
authors.lastname: Barents
authors.forename: J
authors.initials: J
authors.lastname: van der Noordaa
authors.forename: J
authors.initials: J
authors.lastname: ter Schegget
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology* / Q000556:secondary; D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D010386:Pelvic Neoplasms / Q000382:microbiology* / Q000556:secondary; D014412:Tumor Virus Infections / Q000382:microbiology; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
chemical_list: D004279:DNA, Viral