Infusion of fish oil emulsion: effects on platelet aggregation and fatty acid composition in phospholipids of plasma, platelets, and red blood cell membranes in rabbits.
An emulsion of fish oil was manufactured to contain 10 g of fish oil/100 mL. Of this, 3 g were eicosapentaenoic acid (EPA) and 1 g was docosahexaenoic acid (DHA). We administered 100 mL of the emulsion into six rabbits intravenously on days 1, 4, 7, 10, and 13. Blood samples were taken on days 0 and 16. The EPA content in phospholipids of plasma, platelets, and red blood cell (RBC) membranes increased 16, 4, and 5 times, respectively. The DHA content in phospholipids of plasma and RBC membranes increased two times whereas that in platelet phospholipids did not increase significantly. Platelet aggregation induced by collagen (10 micrograms/mL) and ADP (5 microM) was depressed significantly after infusion of the fish oil emulsion. In control experiments with soybean oil emulsion, there were almost no significant changes. Therefore, fish oil emulsion may be beneficial to patients who cannot take n-3 fatty acids orally but need them.
Urakaze
M
M
First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Japan.
Hamazaki
T
T
Makuta
M
M
Ibuki
F
F
Kobayashi
S
S
Yano
S
S
Kumagai
A
A
D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis / Q000097:blood*; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology*; D007262:Infusions, Intravenous; D010743:Phospholipids / Q000032:analysis / Q000097:blood; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D013024:Soybean Oil / Q000008:administration & dosage
D005227:Fatty Acids; D005395:Fish Oils; D010743:Phospholipids; D004281:Docosahexaenoic Acids; D013024:Soybean Oil; D015118:Eicosapentaenoic Acid