Identification of a third nuclear protein-coding gene required specifically for posttranscriptional expression of the mitochondrial COX3 gene is Saccharomyces cerevisiae.
A third nuclear protein-coding gene termed PET122 has been shown to be required for a post-transcriptional step in expression of the mitochondrial COX3 gene is Saccharomyces cerevisiae. pet122 mutants fail to produce cytochrome c oxidase subunit III, which is the polypeptide product of the COX3 gene, but produce normal amounts of mature COX3 mRNA. A strain bearing the pet122-1 allele is amber suppressible and correctly processes the 5' end of COX3 mRNA. Therefore, the PET122 gene product is a protein required for the expression of COX3 at some step after transcription and 5'-end processing of its transcript.
Kloeckener-Gruissem
B
B
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
McEwen
J E
JE
Poyton
R O
RO
D003576:Electron Transport Complex IV / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D008928:Mitochondria / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D005656:Fungal Proteins; D009687:Nuclear Proteins; D003576:Electron Transport Complex IV