Expression and characterization of herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) by recombinant vaccinia virus: neutralization of HSV-1 infectivity with anti-gG antibody.
A recombinant vaccinia virus expressing herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) has been constructed. Cells infected with this recombinant virus (gG-VAC) synthesized glycosylated proteins of 48K, 57K and 61K mol. wt. that were recognized by anti-HSV-1 sera. Rabbits and mice vaccinated with the live recombinant virus produced antibodies that recognized 48K, 57K and 61K mol. wt. proteins in HSV-1-infected cells. The gG polypeptides were present on cytoplasmic and nuclear membranes during infection with both HSV-1 and recombinant vaccinia virus gG-VAC. The 57K and 61K mol. wt. gG polypeptides were present in purified HSV-1 virions and were targets for antibody-mediated complement-dependent virus neutralization.
Sullivan
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Department of Pathology, University of Cambridge, U.K.
Smith
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GL
D000914:Antibodies, Viral / Q000276:immunology*; D002478:Cells, Cultured; D004274:DNA, Recombinant; D008970:Molecular Weight; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology; D018139:Simplexvirus / Q000235:genetics* / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology; D014771:Virion / Q000276:immunology
D000914:Antibodies, Viral; D004274:DNA, Recombinant; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C038535:glycoprotein gC, herpes simplex virus type 1