title: Repetitive cloning of mutant genes using locus-specific sticky ends.
authors.forename: L A
authors.initials: LA
authors.lastname: Chasin
authors.forename: A M
authors.initials: AM
authors.lastname: Carothers
authors.forename: G
authors.initials: G
authors.lastname: Urlaub
mesh_terms: D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D006224:Cricetinae; D003412:Cricetulus; D004262:DNA Restriction Enzymes; D005796:Genes; D009154:Mutation; D009693:Nucleic Acid Hybridization; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014162:Transfection
chemical_list: D013762:Tetrahydrofolate Dehydrogenase; D004262:DNA Restriction Enzymes