An improved procedure for the rapid one-step-cloning of full-length viroid cDNA.
The efficiency of viroid cloning can be increased by three to four orders of magnitude when the synthesis of viroid cDNA is primed in such a way that it carries identical sticky ends on both termini and when the multi-ion transformation is applied.
Puchta
H
H
Max-Planck-Institut für Biochemie, Abteilung Viroidforschung, Martinsried bei München, Federal Republic of Germany.
Sänger
H L
HL
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism; D004526:Efficiency; D004926:Escherichia coli / Q000235:genetics; D014170:Transformation, Genetic; D014772:Viroids / Q000235:genetics*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes