title: Analyses of adenosine and adenine nucleotides in biological materials by fluorescence reaction-high-performance liquid chromatography.
abstract: A previous method of determination of adenine compounds by high-performance liquid chromatography, using bromoacetaldehyde as a fluorescent reagent and a column of Hitachi gel No. 3012-N, was improved and extended to biological materials, especially to measure enzyme activities. A column packed with finer beads, Hitachi gel No. 3013-N, was found to be better than that of No. 3012-N, judging from the analysis time and resolution. ADP, from the hydrolysis of ATP by Na, K-ATPase, was determined quantitatively, and the enzyme activity was inhibited with ouabain. cAMP obtained from ATP by reaction with adenylate cyclase was also determined in the presence of various concentrations of L-epinephrine or sodium fluoride. The ATP levels in human blood were determined, and the cellular levels of ATP and ADP in neuroblastoma N1E 115 were examined as a function of cell growth.
authors.affiliation: Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yoshioka
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: M M
authors.initials: MM
authors.lastname: Abu-Zeid
authors.forename: H
authors.initials: H
authors.lastname: Fujimori
authors.forename: A
authors.initials: A
authors.lastname: Fuke
authors.forename: K
authors.initials: K
authors.lastname: Hirai
authors.forename: A
authors.initials: A
authors.lastname: Goto
authors.forename: M
authors.initials: M
authors.lastname: Ishii
authors.forename: T
authors.initials: T
authors.lastname: Sugimoto
authors.forename: H
authors.initials: H
authors.lastname: Parvez
mesh_terms: D000225:Adenine / Q000097:blood; D000227:Adenine Nucleotides / Q000032:analysis*; D000241:Adenosine / Q000032:analysis*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004910:Erythrocyte Membrane / Q000032:analysis; D006801:Humans; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013050:Spectrometry, Fluorescence
chemical_list: D000227:Adenine Nucleotides; D010042:Ouabain; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000262:Adenylyl Cyclases; D000254:Sodium-Potassium-Exchanging ATPase; D000225:Adenine; D000241:Adenosine