Nucleotide sequencing and expression of the fadL gene involved in long-chain fatty acid transport in Escherichia coli.
The fadL gene of Escherichia coli codes for an outer membrane protein involved in long-chain fatty acid transport. Its product was purified from outer membrane proteins. We determined the nucleotide sequence of a 2.8-kb chromosomal DNA segment that contains the fadL gene. The fadL gene consists of a 1149-nucleotide coding region and contains a highly hydrophobic polypeptide of 383 amino acids with a calculated molecular weight of 42,380. We have used S1-mapping analysis to identify the transcription initiation site. A region exhibiting extensive dyad symmetry and perfect homology to the catabolite activator protein binding site was detected.
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Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Ghosn
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Nunn
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D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D001692:Biological Transport; D000242:Cyclic AMP / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D011953:Receptors, Cyclic AMP / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids; D011953:Receptors, Cyclic AMP; C065691:fadL protein, E coli; D000242:Cyclic AMP; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase