Detection of goat pox antigen and antibody by the counter immunoelectrophoresis test.
The counterimmunoelectrophoresis (CIE) test was standardised for the detection of goat pox antigen and antibody using inactivated antigens. The chloroform inactivated and live antigens were equally sensitive for detection of goat pox precipitins. The precipitinogens of goat pox virus (GPV) were found to be soluble in nature. The CIE test was quick as well as more sensitive than the agar gel precipitation test for detection of GPV antibody/antigen. The CIE employing inactivated antigen has been used for the first time in the detection of GPV antibodies/antigens.
Sharma
B
B
Indian Veterinary Research Institute, Mukteswar-Kumaon.
Negi
B S
BS
Pandey
A B
AB
Bandyopadhyay
S K
SK
Shankar
H
H
Yadav
M P
MP
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D003378:Counterimmunoelectrophoresis / Q000662:veterinary; D005260:Female; D006041:Goats / Q000382:microbiology*; D008297:Male; D011213:Poxviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D011233:Precipitin Tests / Q000662:veterinary; D011237:Predictive Value of Tests
D000914:Antibodies, Viral; D000956:Antigens, Viral