Detection of influenza nucleoprotein antigen in nasal secretions from horses infected with A/equine influenza (H3N8) viruses.
An antigen capture indirect enzyme linked immunosorbent assay (ELISA) was developed to detect influenza nucleoprotein antigen in nasal secretions from horses infected with A/equine/H3N8 viruses. Results from this assay were compared with conventional virus isolation in embryonated hens eggs.
Cook
R F
RF
Equine Virology Unit, Animal Health Trust, Newmarket, Suffolk, U.K.
Sinclair
R
R
Mumford
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006734:Horse Diseases / Q000175:diagnosis* / Q000276:immunology; D006736:Horses; D053123:Influenza A Virus, H3N8 Subtype; D009980:Influenza A virus / Q000276:immunology* / Q000302:isolation & purification; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism*; D009698:Nucleoproteins / Q000032:analysis / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D009698:Nucleoproteins