title: Basic and acidic fibroblast growth factors modulate the epidermal growth factor receptor by a protein kinase C-independent pathway.
abstract: Human acidic and basic fibroblast growth factors (aFGF and bFGF) inhibit epidermal growth factor (EGF) receptor binding in mouse Swiss 3T3 cells. Scatchard analysis indicates that aFGF and bFGF cause a decrease in the high affinity EGF receptor population, similar to that observed for activators of protein kinase C such as phorbol esters, platelet-derived growth factor (PDGF) and bombesin. However, unlike phorbol esters, aFGF and bFGF inhibit EGF binding in protein kinase C-deficient cells. The time course and dose response of inhibition of EGF binding by both aFGF and bFGF are very similar, with an ID50 of approximately 0.10 ng/ml. In contrast to bombesin but like PDGF, neither aFGF nor bFGF act on the EGF receptor through a pertussis toxin-sensitive G protein. These results indicate that both acidic and basic FGF depress high affinity EGF binding in Swiss 3T3 cells with similar potency through a protein kinase C/Gi-independent pathway.
authors.affiliation: Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: K
authors.initials: K
authors.lastname: Hicks
authors.forename: B
authors.initials: B
authors.lastname: Friedman
authors.forename: M R
authors.initials: MR
authors.lastname: Rosner
mesh_terms: D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000187:drug effects / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D051379:Mice; D037342:Pertussis Toxin; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D010982:Platelet-Derived Growth Factor; D010566:Virulence Factors, Bordetella; D015240:Phorbol 12,13-Dibutyrate; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D037342:Pertussis Toxin; D066246:ErbB Receptors; D011493:Protein Kinase C; D001839:Bombesin