Activation of herpes simplex virus 1 gamma 2 genes by viral DNA replication.
The expression of gamma 2 or true gamma genes resident in the herpes simplex virus 1 genome requires functional products of genes expressed earlier in infection and viral DNA synthesis. To determine whether the requirement for viral DNA synthesis for the expression of gamma 2 genes reflects a trans-acting function of a product of one or a few genes made or activated during viral DNA synthesis or whether it reflects a cis effect of DNA synthesis at specific sites, 143 thymidine kinase (TK) minus cells were sequentially infected with a virus carrying a 700-bp deletion of the TK gene and 6 hr later with wild-type or recombinant viruses carrying an alpha-TK or a gamma 2-TK gene in either the presence or absence of viral DNA synthesis. These experiments indicated that the gamma 2-TK gene contained in an unreplicated viral genome was not expressed by trans-acting factors specified before and during DNA synthesis by the first infecting virus, and, therefore, the induction of gamma 2 genes appears to be mediated by a cis-acting function associated with viral DNA synthesis.
Mavromara-Nazos
P
P
Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Illinois 60637.
Roizman
B
B
D004261:DNA Replication; D005786:Gene Expression Regulation; D005814:Genes, Viral; D009690:Nucleic Acid Conformation; D012045:Regulatory Sequences, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; D013937:Thymidine Kinase