title: Telomeric repeat from T. thermophila cross hybridizes with human telomeres.
abstract: The ends (telomeres) of eukaryotic chromosomes must have special features to ensure their stability and complete replication. Studies in yeast, protozoa, slime moulds and flagellates show that telomeres are tandem repeats of simple sequences that have a G-rich and a C-rich strand. Mammalian telomeres have yet to be isolated and characterized, although a DNA fragment within 20 kilobases of the telomeres of the short arms of the human sex chromosomes has been isolated. Recently we showed that a chromosome from the fission yeast Schizosaccharomyces pombe could, in some cases, replicate as an autonomous mini-chromosome in mouse cells. By extrapolation from other systems, we reasoned that mouse telomeres could be added to the S. pombe chromosome ends in the mouse cells. On setting out to test this hypothesis we found to our surprise that the telomeric probe used (containing both the S. pombe and Tetrahymena thermophila repeats) hybridized to a series of discrete fragments in normal mouse DNA and DNA from a wide range of eukaryotes. We show here that the sequences hybridizing to this probe are located at the telomeres of most, if not all, human chromosomes and are similar to the Tetrahymena telomeric-repeat component of the probe.
authors.affiliation: MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburgh, UK.
authors.forename: R C
authors.initials: RC
authors.lastname: Allshire
authors.forename: J R
authors.initials: JR
authors.lastname: Gosden
authors.forename: S H
authors.initials: SH
authors.lastname: Cross
authors.forename: G
authors.initials: G
authors.lastname: Cranston
authors.forename: D
authors.initials: D
authors.lastname: Rout
authors.forename: N
authors.initials: N
authors.lastname: Sugawara
authors.forename: J W
authors.initials: JW
authors.lastname: Szostak
authors.forename: P A
authors.initials: PA
authors.lastname: Fantes
authors.forename: N D
authors.initials: ND
authors.lastname: Hastie
mesh_terms: D000818:Animals; D002875:Chromosomes; D002877:Chromosomes, Human; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012568:Schizosaccharomyces / Q000235:genetics; D012730:Sex Chromosomes; D013768:Tetrahymena / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D004274:DNA, Recombinant; D004247:DNA; D004262:DNA Restriction Enzymes