Effect of isoproterenol and dibutyryl cyclic AMP on thyroxine type-II 5'-deiodinase and N-acetyltransferase activities in rat pineal organ cultures.
The effects of either isoproterenol or dibutyryl cyclic AMP on rat pineal thyroxine type-II 5'-deiodinase (5'-D) and N-acetyltransferase (NAT) activities were studied using an organ culture technique. Moving the animals to light at night dramatically inhibited the NAT with no change in the 5'-D activity. The subsequent incubation of pineal glands with either isoproterenol or dibutyryl cyclic AMP restored the NAT activity and caused a further rise in 5'-D activity. In animals maintained under light conditions during the night, both isoproterenol and dibutyryl cyclic AMP were able to stimulate the 5'-D and NAT activities. The results suggest that the beta-adrenergic activation of the 5'-D activity is mediated by cyclic AMP in the rat pineal gland.
Guerrero
J M
JM
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Santana
C
C
Reiter
R J
RJ
D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology*; D007453:Iodide Peroxidase / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009924:Organ Culture Techniques; D010870:Pineal Gland / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D003994:Bucladesine; D007453:Iodide Peroxidase; D000123:Acetyltransferases; D001191:Arylamine N-Acetyltransferase; D007545:Isoproterenol