GTP-binding protein associated with amino acid binding proteins from olfactory epithelium of skate, Dasyatis pastinaca.
Amino acid binding protein (98 kDa) from olfactory epithelium of skate, Dasyatis pastinaca, form a stable complex with the 56 kDa protein. This complex dissociates in the presence of 10 microM GTP gamma S and 2 mM MgCl2. The 56 kDa protein has a steady-state GTPase activity (15 nmol/min per mg). Binding of amino acids to the 98 kDa protein specifically stimulates GTPase activity of the 56 kDa protein; half-maximal stimulation of GTPase activity is observed at 0.1 microM amino acid.
Novoselov
V I
VI
Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region.
Krapivinskaya
L D
LD
Krapivinsky
G B
GB
Fesenko
E E
EE
D000409:Alanine / Q000494:pharmacology; D000818:Animals; D004848:Epithelium / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D009831:Olfactory Mucosa / Q000378:metabolism*; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D012860:Skates, Fish
D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D000409:Alanine