title: Distribution of alpha 1- and alpha 2-adrenoceptors in brush border and basolateral membranes from rat kidney cortical tubules.
abstract: To examine the localization of alpha-adrenoceptors within the kidney tubule, binding experiments with 125I-HEAT and 3H-yohimbine were performed using cortical homogenates, basolateral membranes (BLM) and brush border membranes (BBM). Bmax values for 125I-HEAT or 3H-yohimbine binding were 2.3- or 2.0-fold that of the homogenate in BBM and 8.2- or 10.7-fold in BLM, respectively. Kd values for 125I-HEAT or 3H-yohimbine were not significantly different between BBM and BLM. These results indicate that both types of alpha-adrenoceptors seem to be localized largely in the BLM.
authors.affiliation: Department of Cardiovascular Dynamics, National Cardiovascular Center Research Institute, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsushima
authors.forename: S
authors.initials: S
authors.lastname: Akabane
authors.forename: M
authors.initials: M
authors.lastname: Kawamura
authors.forename: K
authors.initials: K
authors.lastname: Ito
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D007457:Iodine Radioisotopes; D007684:Kidney Tubules / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D010627:Phenethylamines; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D044024:Tetralones; D015016:Yohimbine
chemical_list: D007457:Iodine Radioisotopes; D010627:Phenethylamines; D011942:Receptors, Adrenergic, alpha; D044024:Tetralones; D015016:Yohimbine; C010652:BE 2254