title: Rat hepatoma (HTC) cell 6-phosphofructo-2-kinase differs from that in liver and can be separated from fructose-2,6-bisphosphatase.
abstract: 6-Phosphofructo-2-kinase was purified from rat liver and hepatoma (HTC) cells. The HTC cell enzyme had kinetic properties different from those of the liver enzyme (more sensitive to inhibition by citrate and not inhibited by sn-glycerol 3-phosphate) and was not a substrate of the cyclic-AMP-dependent protein kinase. Unlike the liver enzyme, which is bifunctional and phosphorylated by fructose 2,6-[2-32P]bisphosphate, the HTC cell enzyme contained no detectable fructose-2,6-bisphosphatase activity and phosphorylation by fructose 2,6-[2-32P]-bisphosphate could not be detected. HTC cell fructose-2,6-bisphosphatase could be separated from 6-phosphofructo-2-kinase activity by purification. Antibodies raised against liver 6-phosphofructo-2-kinase did not precipitate HTC cell fructose-2,6-bisphosphatase whose kinetic properties were completely different from those of the liver enzyme.
authors.affiliation: Hormone and Metabolic Research Unit, Louvain University Medical School, Brussels, Belgium.
authors.forename: A M
authors.initials: AM
authors.lastname: Loiseau
authors.forename: M H
authors.initials: MH
authors.lastname: Rider
authors.forename: D
authors.initials: D
authors.lastname: Foret
authors.forename: G G
authors.initials: GG
authors.lastname: Rousseau
authors.forename: L
authors.initials: L
authors.lastname: Hue
mesh_terms: D000818:Animals; D005636:Fructosephosphates / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000302:isolation & purification* / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats
chemical_list: D005636:Fructosephosphates; C027618:fructose-6-phosphate; D011494:Protein Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases