A new SV40 mutant that encodes a small fragment of T antigen transforms established rat and mouse cells.
We have constructed a new SV40 mutant, T147, that makes a large T antigen that is only 147 amino acids long. We show that the T147 T antigen is a phosphoprotein that is as stable as wild-type T antigen and that the SV40 viral origin binding activity of the T147 T antigen is reduced at least 100-fold relative to that of wild-type T antigen. Most importantly, we demonstrate that cloned T147 DNA transforms rat F111 cells to anchorage independence as efficiently as cloned wild-type SV40 DNA and that cloned T147 DNA also efficiently transforms C3H10T1/2 mouse cells in a focus assay.
Sompayrac
L
L
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.
Danna
K J
KJ
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D002455:Cell Division; D002472:Cell Transformation, Viral; D005347:Fibroblasts; D051379:Mice; D008809:Mice, Inbred C3H; D010750:Phosphoproteins / Q000235:genetics; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics*
D000957:Antigens, Viral, Tumor; D010750:Phosphoproteins