title: Evidence for a gonadotropin-releasing hormone binding protein in goldfish (Carassius auratus) serum.
abstract: The binding of salmon gonadotropin-releasing hormone (sGnRH) and its superactive analog, [D-Arg6, Pro9-NEt]-sGnRH, to a macromolecular component in goldfish serum was studied, using 125I-[D-Arg6, Pro9-NEt]-sGnRH and 125I-sGnRH as labeled ligands. Bound was separated from free labeled ligand by gel filtration with Sephadex G-50. The binding of labeled ligand to goldfish serum was dose-dependent. The results indicate a single class of binding site having low affinity and high capacity. The existence of a GnRH binding protein in serum may, in part, contribute to the long-lasting pharmacological action of GnRHs in goldfish.
authors.affiliation: Department of Zoology, University of Alberta, Edmonton, Canada.
authors.forename: Y P
authors.initials: YP
authors.lastname: Huang
authors.forename: R E
authors.initials: RE
authors.lastname: Peter
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D003530:Cyprinidae / Q000097:blood*; D006054:Goldfish / Q000097:blood*; D007700:Kinetics; D010906:Pituitary Hormone-Releasing Hormones / Q000378:metabolism*; D011966:Receptors, LHRH / Q000097:blood*
chemical_list: D010906:Pituitary Hormone-Releasing Hormones; D011966:Receptors, LHRH