Intranuclear uptake and persistence of biologically active DNA after electroporation of mammalian cells.
Radiolabeled or biologically functional DNA molecules were introduced into cells by electroporation in a variety of forms: double stranded circles, linearized double stranded fragments and single stranded circular molecules. Molecules rapidly entered cells after exposure to a high field-strength electric pulse and then redistributed between the cytoplasm and the nucleus. Maximal intranuclear levels approximated 10(4) molecules per cell. Introduced DNA persisted in a biologically active form with a half-life of 15-24 h. There was no evidence for biologically significant alteration of two double stranded gene sequences. Single stranded DNA molecules also retained biological activity.
Bertling
W
W
Department of Medicine, UCLA School of Medicine 90024.
Hunger-Bertling
K
K
Cline
M J
MJ
D002472:Cell Transformation, Viral; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004558:Electric Stimulation; D005818:Genetic Engineering / Q000379:methods; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency* / Q000235:genetics; D007700:Kinetics; D008214:Lymphocytes
D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase