Suppression of T-lymphocyte blastogenesis by ovine trophoblast protein-1 and human interferon-alpha may be independent of interleukin-2 production.
Cells derived from the trophoblast tissue of a day 15 sheep conceptus released substances that inhibit incorporation of [3H]thymidine into phytohemagglutinin (PHA)-stimulated ovine lymphocytes. This effect was partially reversed by addition of antiserum to ovine trophoblast protein-1 (oTP-1), a major secretory product of day 13-21 sheep conceptuses and a protein structurally and functionally related to alpha-interferons (IFN-alpha). Human IFN-alpha, unlike dexamethasone, inhibits phytohemagglutinin-induced lymphocyte blastogenesis without reducing interleukin-2 (IL-2) production by the cultures, and conditioned medium containing IL-2 does not promote [3H]thymidine incorporation into ovine lymphocytes when oTP-1 is present. Thus, oTP-1, by virtue of being an IFN, may have a local immunomodulatory role by selectively inhibiting the proliferative responses of certain maternal immune cells to IL-2.
Niwano
Y
Y
Department of Dairy Science, University of Missouri, Columbia 65211.
Hansen
T R
TR
Kazemi
M
M
Malathy
P V
PV
Johnson
H D
HD
Roberts
R M
RM
Imakawa
K
K
D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D011257:Pregnancy Proteins / Q000494:pharmacology*; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology*
D007370:Interferon Type I; D007376:Interleukin-2; D011257:Pregnancy Proteins; C020947:interferon tau; D003907:Dexamethasone