Functionally distinct receptors for cholecystokinin and gastrin on dispersed chief cells from guinea pig stomach.
Caerulein, gastrin, and C-terminal fragments of cholecystokinin (CCK) varying in length from eight (CCK-8) to four (CCK-4) amino acids stimulate pepsinogen secretion from dispersed chief cells prepared from guinea pig stomach. C-terminal fragments of CCK containing fewer than four amino acids, even when tested at concentrations as high as 3 mM, do not stimulate pepsinogen secretion. The efficacies of gastrin and the various CCK-related peptides, coupled with the pattern of action of CCK receptor antagonists, indicate that chief cells from guinea pig stomach possess two functionally distinct classes of receptors, C-receptors and G-receptors. The C-receptors can be occupied by caerulein, CCK-8, CCK-7, des(SO3)CCK-8, or des(SO3)CCK-7, and occupation of C-receptors causes full stimulation of pepsinogen secretion. G-receptors can be occupied by gastrin I, gastrin II, CCK-6, CCK-5, or CCK-4, and occupation of G-receptors causes stimulation of pepsinogen secretion that is 60% of maximal.
Cherner
J A
JA
Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Sutliff
V E
VE
Grybowski
D M
DM
Jensen
R T
RT
Gardner
J D
JD
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D002108:Ceruletide / Q000494:pharmacology; D002766:Cholecystokinin / Q000494:pharmacology; D020109:Devazepide; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism* / Q000494:pharmacology; D006168:Guinea Pigs; D008297:Male; D010435:Pepsinogens / Q000037:antagonists & inhibitors / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D011377:Proglumide / Q000031:analogs & derivatives; D011956:Receptors, Cell Surface / Q000378:metabolism* / Q000502:physiology; D011949:Receptors, Cholecystokinin / Q000378:metabolism* / Q000502:physiology; D013270:Stomach / Q000166:cytology
D001570:Benzodiazepinones; D005755:Gastrins; D010435:Pepsinogens; D010455:Peptides; D011956:Receptors, Cell Surface; D011949:Receptors, Cholecystokinin; D002108:Ceruletide; D002766:Cholecystokinin; D011377:Proglumide; D020109:Devazepide