title: Characterization of human papillomavirus type 45, a new type 18-related virus of the genital tract.
abstract: DNA of human papillomavirus (HPV) type 45, a new HPV type 18-related papillomavirus of the genital tract, was cloned from a recurrent cervical lesion displaying mild to moderate dysplasia with koilocytosis. HPV-45 DNA was identified in paraffin sections of biopsies of both the initial and recurrent lesions of the patient, taken 7 months apart. HPV-45 DNA hybridized efficiently to that of many different HPV types under low and moderate stringency conditions (Tm - 37 degrees C to Tm - 25 degrees C) but with only HPV-18 DNA under high stringency conditions (Tm - 17 degrees C). HPV-45 DNA was distinguished from HPV-18 DNA by (i) differences in restriction enzyme digest patterns, (ii) lack of hybridization at Tm - 17 degrees C between HPV-18 and some fragments of HPV-45, (iii) a value of 25% in liquid reassociation kinetics between HPV-18 and HPV-45 and (iv) differences in intensities of hybridization with selected tissue DNAs. The prevalence of HPV-45 infection in the genital tract was low. In tests of over 600 tissue DNAs from female genital tract lesions, HPV-45 sequences were detected in three additional tissues, one each of invasive cervical carcinoma, condyloma, and normal cervical epithelium. HPV-45 is a newly recognized papillomavirus which rarely infects the genital tract and is associated with lesions across a wide histological spectrum.
authors.affiliation: Department of Immunology and Infectious Diseases, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205.
authors.forename: Z S
authors.initials: ZS
authors.lastname: Naghashfar
authors.forename: N B
authors.initials: NB
authors.lastname: Rosenshein
authors.forename: A T
authors.initials: AT
authors.lastname: Lorincz
authors.forename: J
authors.initials: J
authors.lastname: Buscema
authors.forename: K V
authors.initials: KV
authors.lastname: Shah
mesh_terms: D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D002577:Uterine Cervical Diseases / Q000382:microbiology*
chemical_list: D004262:DNA Restriction Enzymes