[Effective method of introducing plasmid DNA into eukaryotic cells by using liposomes for producing vaccinia virus recombinants].
The method of transfection of eukaryotic cells by plasmid DNA and production of recombinant vaccinia virus is described. The method is based on DNA encapsulation into reverse evaporation vesicles containing gangliosides, with a subsequent incubation of liposomes with vaccinia-infected cells in the presence of UV-inactivated Sendai virus. The recombinant DNA of vaccinia virus containing part of env gene of HTLV-3/LAV virus was obtained.
Slepushkin
V A
VA
Bukrinskiĭ
M I
MI
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D005732:Gangliosides / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D006678:HIV / Q000235:genetics; D008081:Liposomes / Q000008:administration & dosage*; D010222:Parainfluenza Virus 1, Human / Q000235:genetics / Q000528:radiation effects; D010957:Plasmids; D014162:Transfection; D014466:Ultraviolet Rays; D014616:Vaccinia virus / Q000235:genetics*; D014709:Vero Cells; D014776:Virus Cultivation
D004274:DNA, Recombinant; D004279:DNA, Viral; D005732:Gangliosides; D008081:Liposomes
Effektivnyĭ metod vvedeniia plazmidnoĭ DNK v éukarioticheskie kletki s pomoshch'iu liposom dlia polucheniia rekombinantov virusa ospovaktsiny.