title: Yeast calmodulin: structural and functional differences compared with vertebrate calmodulin.
abstract: Calmodulin of the baker's yeast (Saccharomyces cerevisiae) showed a similar affinity for Ca2+ to that of vertebrate calmodulin. The maximum binding number of Ca2+ to yeast calmodulin was, however, 3 mol/mol, which is lower than that of vertebrate calmodulin (4 mol/mol). The same maximum activity of porcine brain phosphodiesterase was attained when 100 times higher concentration of yeast calmodulin than that of vertebrate calmodulin was added. On the other hand, the maximum activation of chicken gizzard myosin light chain kinase was attained with 1,000 times higher concentration of yeast calmodulin than that of vertebrate calmodulin, and the maximum activity with yeast calmodulin was less than 1/5 of that with vertebrate calmodulin. Several amino acid substitutions observed in the yeast calmodulin, particularly at the alpha-helical rod connecting the two globular domains, may affect the interaction mode of various target enzymes with this calmodulin.
authors.affiliation: Department of Chemistry, Faculty of Science, Hokkaido University.
authors.forename: Y
authors.initials: Y
authors.lastname: Luan
authors.forename: I
authors.initials: I
authors.lastname: Matsuura
authors.forename: M
authors.initials: M
authors.lastname: Yazawa
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Yagi
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002147:Calmodulin / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D002645:Chickens; D004789:Enzyme Activation / Q000187:drug effects; D005895:Gizzard, Avian / Q000032:analysis; D008970:Molecular Weight; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000596:Amino Acids; D002147:Calmodulin; D009219:Myosin-Light-Chain Kinase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D002118:Calcium