Developmental changes in immunoreactive content of novel pituitary protein 7B2 in human pancreas and its identification in pancreatic tumors.
Developmental changes in concentration of a novel pituitary protein (7B2) were studied immunochemically in the human pancreas from 20 wk of gestation to 4 mo after birth. The concentrations of 7B2 in pancreatic islet tumors and in nesidioblastosis were investigated also. Significant quantities of 7B2-like immunoreactivity (IR-7B2) were found in all developmental stages studied. The highest concentrations of IR-7B2 were found at term [215.4 +/- 40.0 vs. 28.3 +/- 4.4 pmol/g (adult levels), P less than .001]. A high incidence of elevated IR-7B2 concentration in pancreatic islet tumors and nesidioblastosis was found (10 of 12 insulinomas, 5 of 8 glucagonomas, and in all 3 pancreases with nesidioblastosis). Gel-permeation chromatography on Sephadex G-100 showed two immunoreactive peaks in all extracts studied. The main peak (Kav 0.30) of IR-7B2 corresponded to that found in the porcine pituitary gland. The high incidence of elevated IR-7B2 concentrations in pancreatic islet tumors and the increase in IR-7B2 concentrations in the term pancreas and particularly in nesidioblastosis suggest that the novel protein 7B2 may serve as an islet marker.
Suzuki
H
H
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
Christofides
N D
ND
Chretien
M
M
Seidah
N G
NG
Polak
J M
JM
Bloom
S R
SR
D007516:Adenoma, Islet Cell / Q000032:analysis*; D000328:Adult; D000375:Aging; D005260:Female; D005333:Fetus; D005865:Gestational Age; D005935:Glucagonoma / Q000032:analysis*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007340:Insulinoma / Q000032:analysis*; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010179:Pancreas / Q000032:analysis / Q000196:embryology / Q000254:growth & development*; D010182:Pancreatic Diseases / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000032:analysis*; D010907:Pituitary Hormones / Q000032:analysis*; D011247:Pregnancy; D012016:Reference Values
D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010907:Pituitary Hormones; C497678:SCG5 protein, human