title: Isolation and characterization of the porcine nuclear factor I (NFI) gene.
abstract: This study describes the isolation of a major portion of the gene for nuclear factor I (NFI) including its 5'-flanking region with transcriptional start sites. We screened a porcine liver, genomic DNA library in phage EMBL3A with synthetic oligonucleotides derived from tryptic and cyanogen-bromide peptide sequences obtained from purified NFI protein. The NFI gene is present as a single copy in porcine DNA.
authors.affiliation: Institut für Biochemie der Universität München, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Meisterernst
authors.forename: L
authors.initials: L
authors.lastname: Rogge
authors.forename: C
authors.initials: C
authors.lastname: Donath
authors.forename: I
authors.initials: I
authors.lastname: Gander
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: R
authors.initials: R
authors.lastname: Mertz
authors.forename: T
authors.initials: T
authors.lastname: Dobner
authors.forename: R
authors.initials: R
authors.lastname: Föckler
authors.forename: G
authors.initials: G
authors.lastname: Stelzer
authors.forename: E L
authors.initials: EL
authors.lastname: Winnacker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D004720:Endonucleases; D005091:Exons; D005796:Genes; D007438:Introns; D008099:Liver / Q000032:analysis; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009693:Nucleic Acid Hybridization; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases