Sequences between the internal control regions of tRNAArg of Drosophila melanogaster influence stimulation of transcription of the 5' flanking DNA.
Recombinants between 5' deletion mutants of a tRNA(3bVal) gene which is inactive as an in vitro transcription template and a tRNAArg gene, which is an active in vitro template were made. The 5' flanking region of tRNA(Arg) including 36 nucleotides of the coding sequence of the gene stimulated transcription of the tRNA(3bVal), deleted to the +17 position, gene by over 50 fold. When the 5' flanking region of the tRNA(Arg) gene included 22 nucleotides of the coding sequence stimulation was reduced by a factor of 3. Thus the sequences between +22 and +36 of tRNA(Arg) are required to permit maximum stimulation of tRNA(3bVal) in vitro template activity.
Horvath
D
D
Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
Spiegelman
G B
GB
D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics*; D004720:Endonucleases / Q000494:pharmacology; D005796:Genes; D066298:In Vitro Techniques; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012347:RNA, Transfer, Arg / Q000235:genetics*; D012366:RNA, Transfer, Val / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012345:RNA, Transfer, Amino Acid-Specific; D012347:RNA, Transfer, Arg; D012366:RNA, Transfer, Val; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases