Over-expression of natural and variant human H-chain ferritins in E. coli.
The natural human H-chain ferritin was expressed in E. coli using a multi-copy expression vector containing the lambda pL promoter. A variant H-ferritin, having an altered N-terminus, was also produced. These proteins are overproduced (greater than 30% of the soluble protein), correctly assembled into its 24-subunit shell, and able to bind iron. The identity of the products was confirmed using an antibody specific for H-ferritin.
Prozzi
D
D
Unit of Biochemistry, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
Crichton
R R
RR
Davison
J
J
D010582:Bacteriophage lambda / Q000235:genetics; D003062:Codon; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005293:Ferritins / Q000235:genetics*; D005786:Gene Expression Regulation; D005818:Genetic Engineering; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic
D003062:Codon; D004274:DNA, Recombinant; D005293:Ferritins; D004262:DNA Restriction Enzymes