Role of replication in IS102-mediated deletion formation.
Intramolecular transposition produces replicon dissociation in a bireplicon; this reaction is homologous to the well-characterized IS-associated deletions in the case of a monoreplicon. However the frequencies at which these two reactions occur differ by a factor of more than 10(2) in favor of deletion formation. This raises the question of how these deletions occur. We show that the presence of a productive replication on the fragment to be deleted interferes with deletion formation. Our results also suggest that the deleted fragment is not degraded during deletion formation.
Bernardi
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F
Laboratoire d'Enzymologie du CNRS, Gif sur Yvette, France.
Bernardi
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A
D002872:Chromosome Deletion; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes