Transfer of condensed viral DNA into eukaryotic cells using proteoliposomes.
High-molecular-weight viral DNAs have been packed into proteoliposomes prepared by reverse-phase evaporation followed by phospholipid membrane targeting by influenza virus glycoprotein bound to hydrophobic 'anchors'. DNA has been encapsulated in the form of spermine condensates--toroidal structures sized approx. 0.1 micron, resistant to ultrasound. The efficiency of entrapping into liposomes reached 30% for condensed DNA of Mr up to 3 X 10(7). Specific infectivity of simian virus 40 DNA and simian adenovirus DNA packed into such proteoliposomes was 50- to 100-fold higher than that shown by free DNA preparations under Ca.phosphate-precipitation conditions.
Tikchonenko
T I
TI
Institute of Biotechnology for Agriculture, Academy of Agricultural Sciences, Moscow, U.S.S.R.
Glushakova
S E
SE
Kislina
O S
OS
Grodnitskaya
N A
NA
Manykin
A A
AA
Naroditsky
B S
BS
D000261:Adenoviruses, Simian / Q000235:genetics; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics*; D005814:Genes, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000235:genetics*; D009980:Influenza A virus / Q000235:genetics*; D008081:Liposomes; D011510:Proteolipids; D013539:Simian virus 40 / Q000235:genetics; D013096:Spermine / Q000494:pharmacology; D014162:Transfection
D004279:DNA, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D008081:Liposomes; D011510:Proteolipids; C020485:proteoliposomes; D013096:Spermine