title: Lipoxygenase products in experimental septic arthritis in dogs.
abstract: Induction of an experimental septic arthritis was performed in the juvenile dog knee by an intra-articular injection with Staphylococcus aureus. From plasma, lipoxygenase (LO) products of arachidonic acid were separated by reversed-phase high-performance liquid chromatography (RP-HPLC) after extraction of lipids. The plasma samples did not contain UV-detectable amounts of LO products other than the LTB4 metabolite 20-COOH-LTB4, and 12-HETE. Small amounts of chemokinetically active material were found coeluting with the eluate fraction of 20-COOH-LTB4, LTB4 and 12-HETE after RP-HPLC. After 48 hours of septic arthritis, resulting in a massive acute joint inflammation, no significant changes in LO products were observed.
authors.affiliation: Department of Pediatrics, Aarhus Kommunehospital, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Herlin
authors.forename: K
authors.initials: K
authors.lastname: Fogh
authors.forename: V E
authors.initials: VE
authors.lastname: Knudsen
authors.forename: E S
authors.initials: ES
authors.lastname: Hansen
authors.forename: E
authors.initials: E
authors.lastname: Bunger
authors.forename: H
authors.initials: H
authors.lastname: Ewald
authors.forename: I
authors.initials: I
authors.lastname: Holm
authors.forename: K
authors.initials: K
authors.lastname: Kragballe
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood; D001170:Arthritis, Infectious / Q000201:enzymology* / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006614:Hindlimb; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D007975:Leukotriene B4 / Q000097:blood* / Q000502:physiology; D008055:Lipids / Q000302:isolation & purification; D008084:Lipoxygenase / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology; D013203:Staphylococcal Infections / Q000201:enzymology* / Q000276:immunology
chemical_list: D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D008055:Lipids; D007975:Leukotriene B4; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008084:Lipoxygenase