Chemical reactions within DNA duplexes. Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent.
Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in aqueous solution. Optimum conditions for this vigorous and effective reaction were developed. CNBr proved to be useful for incorporation of phosphoramidate or pyrophosphate internucleotide bonds in DNA duplexes.
Sokolova
N I
NI
A.N. Belozersky Laboratory, Moscow State University, USSR.
Ashirbekova
D T
DT
Dolinnaya
N G
NG
Shabarova
Z A
ZA
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003488:Cyanogen Bromide / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D011088:DNA Ligases / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D009838:Oligodeoxyribonucleotides / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D011088:DNA Ligases; D003488:Cyanogen Bromide