Complete nucleotide sequence of reovirus L2 gene and deduced amino acid sequence of viral mRNA guanylyltransferase.
Reovirus mRNAs synthesized by the virion-associated RNA polymerase contain a 5'-terminal cap that is added to nascent transcripts by polypeptide lambda 2, a structural component of virions encoded by double-stranded RNA genome segment L2. The complete, 3916-nucleotide sequence of a full-length reovirus type 3 L2 DNA clone was determined by the dideoxy chain terminator method. The sequence has a single long open reading frame extending from the second A-T-G at nucleotide 14 to a termination codon at position 3881. On this basis, the 1289-amino acid sequence of polypeptide lambda 2, the reovirus mRNA guanylyltransferase, was deduced and compared to other GTP-binding proteins. Two different, lysine-containing lambda 2 peptide sequences closely resemble predicted amino acid stretches in vaccinia virus guanylyltransferase and potentially form part of active sites in the viral mRNA capping enzymes.
Seliger
L S
LS
Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08855-0759.
Zheng
K
K
Shatkin
A J
AJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D012087:Reoviridae / Q000235:genetics*
D003062:Codon; D004279:DNA, Viral; D012333:RNA, Messenger; D009713:Nucleotidyltransferases; C020842:guanylyltransferase