Recovery of polypeptides from polyacrylamide gels by electrophoretic elution in a centrifugation concentrator.
A method for recovery of polypeptides from polyacrylamide gels by electrophoretic elution in a commercially available concentrator, the Amicon-Centricon sample reservoir, has been devised. The recoveries were greater than 90% with four different polypeptides tested (12.5 to 80 kDa). After elution, sample concentration or salt exchange can be carried out without sample transfer. There were no loss of sample during the postelution procedures when the elution buffer was replaced by 0.01 or 0.05% sodium dodecyl sulfate.
Ihara
S
S
Department of Plastic Surgery, Kitasato University School of Medicine, Kanagawa, Japan.
Suzuki
H
H
Kawakami
M
M
D003574:Cytochrome c Group / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015852:Eukaryotic Initiation Factor-2; D010448:Peptide Initiation Factors / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D014461:Ultracentrifugation
D003574:Cytochrome c Group; D015852:Eukaryotic Initiation Factor-2; D010448:Peptide Initiation Factors; D010455:Peptides; D011506:Proteins; D012967:Sodium Dodecyl Sulfate