Atrial natriuretic factor inhibits phosphate uptake in opossum kidney cells: as a model of renal proximal tubules.
The cultured renal cell, an opossum kidney (OK) cell line, which contains several features characteristic of proximal tubular cells, was utilized to examine the direct effects of atrial natriuretic factor (ANF) and cyclic GMP (cGMP) on phosphate uptake. ANF at 2 x 10(-7) M significantly inhibited phosphate uptake by 10.1% of control (P less than 0.01). Incubation of the cells with ANF (10(-8) to 10(-6) M) resulted in an increment of intracellular cGMP in a dose dependent fashion. Exogenous addition of 8-bromo-cGMP (10(-4) M) also significantly inhibited phosphate uptake by 14.6%. These results suggest that ANF directly inhibits phosphate transport in renal proximal tubular cells, probably through stimulation of cGMP production.
Nakai
M
M
Department of Medicine, Kobe University School of Medicine, Japan.
Fukase
M
M
Kinoshita
Y
Y
Fujita
T
T
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism; D009893:Opossums; D010710:Phosphates / Q000378:metabolism*; D013997:Time Factors
D010710:Phosphates; C016276:8-bromocyclic GMP; D009320:Atrial Natriuretic Factor; D000242:Cyclic AMP; D006152:Cyclic GMP