title: An investigation of glucose uptake in relation to steroidogenesis in rat testis and tumour Leydig cells.
abstract: The mechanisms of the requirement of glucose for steroidogenesis were investigated by monitoring the uptake of the glucose analogue 2-deoxy-D-glucose by rat testis and tumour Leydig cells. The characteristics of glucose transport in both of these cell types were found to resemble those of the facilitated-diffusion systems for glucose found in most other mammalian cells. The Leydig cells took up 2-deoxy-D-glucose but not L-glucose, and the uptake was inhibited by both cytochalasin B and forskolin. In the presence of luteinizing hormone, the rate of 2-deoxy-D-glucose uptake by both cell types was increased by approx. 50%. In addition to D-glucose, it was shown that the Leydig cells could also utilize 3-hydroxybutyrate or glutamine to maintain steroidogenesis.
authors.affiliation: Department of Biochemistry, Royal Free Hospital School of Medicine (University of London), U.K.
authors.forename: P
authors.initials: P
authors.lastname: Amrolia
authors.forename: M H
authors.initials: MH
authors.lastname: Sullivan
authors.forename: D
authors.initials: D
authors.lastname: Garside
authors.forename: S A
authors.initials: SA
authors.lastname: Baldwin
authors.forename: B A
authors.initials: BA
authors.lastname: Cooke
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003837:Deoxy Sugars / Q000493:pharmacokinetics*; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D051381:Rats; D013736:Testicular Neoplasms / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis*
chemical_list: D003837:Deoxy Sugars; D005576:Colforsin; D003571:Cytochalasin B; D013739:Testosterone; D003994:Bucladesine; D007986:Luteinizing Hormone; D003847:Deoxyglucose