Superoxide production from nonenzymatically glycated protein.
Nonenzymatically glycated human serum albumin and glycated poly-lysine(Lys) in vitro brought about the reduction of nitroblue tetrazolium and ferricytochrome c at pH 9.06 and pH 7.8, respectively. This reduction was inhibited partially by superoxide dismutase (SOD). Glycated poly-Lys caused the oxidation of NADH in the presence of LDH at pH 7.0 which was completely inhibited by SOD. Glycated material was found to function both as a reductant and an oxidant. The reactivity of glycated material is discussed and a possible mechanism by which superoxide is produced is proposed. Results may give a clue to diabetic complications.
Sakurai
T
T
Tokyo College of Pharmacy, Japan.
Tsuchiya
S
S
D003574:Cytochrome c Group / Q000378:metabolism; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D066298:In Vitro Techniques; D049449:Luminescence; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D011107:Polylysine / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*; D013438:Sulfhydryl Compounds; D013481:Superoxides / Q000378:metabolism*; D000093423:Glycated Serum Albumin
D003574:Cytochrome c Group; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D013481:Superoxides; D011107:Polylysine; D009580:Nitroblue Tetrazolium; D000093423:Glycated Serum Albumin