Evaluation of a latex test for rotavirus detection.
A latex agglutination (LA) test (Slidex Rota-Kit; bioMérieux, Marcy-l'Etoile, France) was a rapid, easily used method for detecting rotavirus (RV) in pediatric fecal specimens. With 45 RV-positive and 50 RV-negative diarrhea specimens, the sensitivity of the LA test was 82%, and the specificity was 100%. Six other specimens produced indeterminate results. The frequency of positive LA tests appeared to be proportional to the concentration of virions in the stool.
Brandt
C D
CD
Children's Hospital National Medical Center, Washington, D.C. 20010.
Arndt
C W
CW
Evans
G L
GL
Kim
H W
HW
Stallings
E P
EP
Rodriguez
W J
WJ
Parrott
R H
RH
D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007841:Latex Fixation Tests; D008854:Microscopy, Electron; D011237:Predictive Value of Tests; D011897:Random Allocation; D011933:Reagent Kits, Diagnostic; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000382:microbiology*
D011933:Reagent Kits, Diagnostic