title: A new approach in recognition of heterochromatic regions of human chromosomes by means of restriction endonucleases.
abstract: The heteromorphisms of C-band regions of human chromosomes are evaluated by means of restriction endonucleases AluI, DdeI, MboI, and RsaI. Every chromosome exhibits heteromorphic markers of the C-band regions except chromosome 8. Each enzyme was found to be highly characteristic in its staining profile, a result that clearly suggests the diversity of heterochromatin. The inherent C-band-region heterochromatin variability that is revealed by these enzymes provides a valuable tool in identifying markers as compared with other previously described techniques.
authors.affiliation: Division of Medical Genetics, Beth Israel Medical Center, Mount Sinai School of Medicine, New York.
authors.forename: A
authors.initials: A
authors.lastname: Babu
authors.forename: A K
authors.initials: AK
authors.lastname: Agarwal
authors.forename: R S
authors.initials: RS
authors.lastname: Verma
mesh_terms: D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D004262:DNA Restriction Enzymes; D006570:Heterochromatin / Q000235:genetics* / Q000648:ultrastructure; D006801:Humans; D007621:Karyotyping
chemical_list: D006570:Heterochromatin; D004262:DNA Restriction Enzymes