Synthesis of PDGF by cultured human T cells transformed with HTLV-I and II.
Human T cells immortalized by human T lymphotropic virus (HTLV)-I and HTLV-II express the genes encoding both chains of platelet-derived growth factor (PDGF). These cells produce biologically active PDGF-like molecules of 31 and 32 kD, identified in the conditioned media of the virus-infected cells by direct immunoprecipitation, under non-reducing conditions, with antisera to PDGF. Upon reduction, the PDGF-like molecules are converted to mitogenically inactive single chains of 14 and 15 kD. The HTLV-transformed cells produce PDGF-like molecules regardless of their ability to produce viral particles. No PDGF-related mRNAs, PDGF-like proteins, nor PDGF-like mitogenic activity were detected in normal human T cells stimulated by phytohemagglutinin or in a T cell line which is not infected by HTLV. It is possible that one of the pathways of immortalization of T cells by HTLV-I or II might be by activation of PDGF-related genes.
Pantazis
P
P
Center for Blood Research, Boston, Massachusetts 02115.
Sariban
E
E
Bohan
C A
CA
Antoniades
H N
HN
Kalyanaraman
V S
VS
D002472:Cell Transformation, Viral; D004247:DNA / Q000096:biosynthesis; D017977:Deltaretrovirus; D005786:Gene Expression Regulation; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000502:physiology; D017479:Receptors, Platelet-Derived Growth Factor; D013601:T-Lymphocytes / Q000502:physiology*
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D004247:DNA; D017479:Receptors, Platelet-Derived Growth Factor