Inhibition of ovulation, steroidogenesis and collagenolytic activity in rabbits by sulpiride-induced hyperprolactinaemia.
Ovulation induced by hCG in rabbits was reduced significantly (P less than 0.005) by sulpiride-induced hyperprolactinaemia. The pre- and post-ovulatory increases in peripheral and ovarian venous progesterone (but not oestradiol or testosterone) were suppressed in the treated animals. The condition of hyperprolactinaemia also prevented the usual changes in 2,4-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH peptidase (DNP-peptidase) and alpha-N-benzoyl-DL-Arg-beta-naphthylamide hydrolase (BANA-hydrolase) activities in follicular tissue that had been stimulated by an ovulatory dose of hCG. These results suggest that inhibition of progesterone production and collagenolytic enzyme activity by sulpiride-induced hyperprolactinaemia may be responsible for the ovulatory dysfunction that occurs when a mammal has a high level of circulating prolactin.
Lin
K C
KC
Department of Gynaecology and Obstetrics, Kyoto University Faculty of Medicine, Japan.
Kawamura
N
N
Okamura
H
H
Mori
T
T
D000818:Animals; D056655:Cathepsin H; D002403:Cathepsins / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism*; D003546:Cysteine Endopeptidases; D005260:Female; D006966:Hyperprolactinemia / Q000139:chemically induced / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism; D006080:Ovarian Follicle / Q000201:enzymology; D010060:Ovulation / Q000187:drug effects*; D011374:Progesterone / Q000097:blood*; D011817:Rabbits; D013469:Sulpiride / Q000494:pharmacology*
D006063:Chorionic Gonadotropin; D011374:Progesterone; D013469:Sulpiride; D003094:Collagen; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D056655:Cathepsin H; D003012:Microbial Collagenase