Immunoaffinity purification of a 72K early antigen of human cytomegalovirus: analysis of humoral and cell-mediated immunity to the purified polypeptide.
An early antigen of human cytomegalovirus (HCMV) was purified from infected cells as a 72K polypeptide by immunoaffinity chromatography using a monoclonal antibody. It was located in both nucleus and cytoplasm, and was non-glycosylated and undetectable on the surface of infected cells. Known seropositive subjects had antibody against the purified protein and it elicited proliferative T cell responses in 10 of 16 subjects. Five of 14 T cell lines established in response to the purified protein were predominantly CD8+ and of these two showed major histocompatibility complex-restricted cytotoxicity against HCMV-infected cells. This provides further evidence that antigens expressed at early times may be targets for the immune response during persistent HCMV infection.
Rodgers
B
B
Department of Medicine, Royal Postgraduate Medical School, London, U.K.
Borysiewicz
L
L
Mundin
J
J
Graham
S
S
Sissons
P
P
D000917:Antibody Formation; D000936:Antigen-Antibody Complex / Q000032:analysis; D000954:Antigens, Surface / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D003587:Cytomegalovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts; D006801:Humans; D017874:Immediate-Early Proteins; D007111:Immunity, Cellular; D008970:Molecular Weight
D000936:Antigen-Antibody Complex; D000954:Antigens, Surface; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus