[Specific chemical modification of cytidine in T4 DNA by a spin label].
A procedure for selective modification of DNA from T4 phage non-glucosylated mutant by the spin label--N(2,2',5,5') tetramethyl-3-carboxypyrrolidine-1-oxyl)-imidazole was developed. The spin label was shown to interact with hydroxyl groups of 5-hydroxymethyl-2 deoxycytidines. The modification does not affect the secondary structure of DNA, its conformation or template properties in a cell-free system of RNA synthesis.
Kamzolova
S G
SG
D003562:Cytidine / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy; D013113:Spin Labels; D013604:T-Phages / Q000032:analysis*
D004279:DNA, Viral; D013113:Spin Labels; D003562:Cytidine
Spetsificheskaia khimicheskaia modifikatsiia tsitidinovykh zven'ev T4-DNK spinovoĭ metkoĭ.