Specific binding of [3H]retinoids to cellular retinoid-binding proteins.
The specific binding of [3H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [3H]retinoids with or without competitors for 2-4 h, bound ligands were separated from free by filtration using cold acetone. Nonspecific binding of the ligands was reduced sufficiently to allow measurement of specific binding of [3H]retinoids to cellular retinoid-binding proteins. This method has the advantages of being rapid and practical and giving reproducible results.
Ninomiya
M
M
National Cancer Center Research Institute, Tokyo, Japan.
Suganuma
M
M
Paik
N S
NS
Muto
Y
Y
Fujiki
H
H
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D007700:Kinetics; D018168:Receptors, Retinoic Acid; D012177:Retinol-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D014212:Tretinoin / Q000378:metabolism*; D014316:Tritium; D014801:Vitamin A / Q000378:metabolism*
D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid; D012177:Retinol-Binding Proteins; D014316:Tritium; D014801:Vitamin A; D014212:Tretinoin