title: Characterization of the gene for fructose-1,6-bisphosphatase from Saccharomyces cerevisiae and Schizosaccharomyces pombe. Sequence, protein homology, and expression during growth on glucose.
abstract: We have determined the nucleotide sequence of the gene for fructose-1,6-bisphosphatase from both Saccharomyces cerevisiae and Schizosaccharomyces pombe. The predicted protein sequence for fructose-1,6-bisphosphatase from S. cerevisiae contains 347 amino acids and has a molecular weight of 38,100; that from S. pombe, contains 346 amino acids and has a molecular weight of 38,380. Comparison of these amino acid sequences with each other and that of pig kidney fructose-1,6-bisphosphatase shows several regions of strong homology separated by regions of divergence. These homologous regions are likely candidates for functional domains. A gene cassette was constructed for fructose-1,6-bisphosphatase from S. cerevisiae and the gene cassette expressed from the regulated PHO5 and GAL1 promoters of yeast. Yeast cells expressing fructose-1,6-bisphosphatase, while growing on glucose, accumulated large amounts of enzyme intracellularly, suggesting that glucose-regulated proteolytic inactivation does not operate efficiently under these conditions. Growth on glucose was not inhibited by the expression of fructose 1,6-bisphosphatase.
authors.affiliation: Genetics Institute, Inc., Cambridge, Massachusetts 02140.
authors.forename: D T
authors.initials: DT
authors.lastname: Rogers
authors.forename: E
authors.initials: E
authors.lastname: Hiller
authors.forename: L
authors.initials: L
authors.lastname: Mitsock
authors.forename: E
authors.initials: E
authors.lastname: Orr
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D005947:Glucose / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D004718:Saccharomycetales / Q000201:enzymology*; D012568:Schizosaccharomyces / Q000201:enzymology*; D013552:Swine
chemical_list: D006597:Fructose-Bisphosphatase; D005947:Glucose