The effects of newborn calf serum and foetal bovine serum on the Na+ + K+-activated adenosine triphosphatase activity in 3T3 and SV3T3 cells grown to confluency.
The effects of cell density and growth in 10% foetal bovine serum and 10% newborn calf serum on the activity of the enzyme (Na+ + K+)-ATPase were studied in 3T3 and SV3T3 cells. The enzyme activity decreases in 3T3 cells grown in foetal bovine serum as the cells approach confluency while in those grown in newborn calf serum the enzyme activity increases. The (Na+ + K+)-ATPase activity does not change with increase in cell density in SV3T3 cells grown in foetal bovine serum while the enzyme activity in those grown in newborn calf serum increases with increase in cells density up to about 1.35 x 10(5) cells/sq. cm. and then decreases with further increase in cell number. At confluency it was found that the enzyme activity is higher in the SV3T3 as compared to the 3T3 cells when the cells were grown in 10% foetal bovine serum, whereas in those grown in 10% newborn calf serum the enzyme activity is higher in the 3T3 as compared to the SV3T3 cells.
Tenang
E M
EM
Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Penang.
McCaldin
B
B
D000818:Animals; D001769:Blood / Q000378:metabolism*; D002417:Cattle; D002460:Cell Line / Q000187:drug effects / Q000201:enzymology*; D002461:Cell Line, Transformed / Q000187:drug effects / Q000201:enzymology; D003470:Culture Media; D005312:Fetal Blood / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D003470:Culture Media; D000254:Sodium-Potassium-Exchanging ATPase