Binding of integration host factor (IHF) to the ilvGp1 promoter of the ilvGMEDA operon of Escherichia coli K12.
Crude extracts of Escherichia coli K-12 were found to bind DNA restriction fragments containing ilvGp1. Our analysis using a series of restriction fragments and a BamHI linker mutation indicate that a factor binds to ilvGp1 or adjacent to it. Analysis with mutant strains of E. coli K-12 and purified IHF indicate that IHF binds to ilvGp1. Furthermore, both analysis in vivo and in vitro indicate that IHF precludes transcription from ilvGp1.
Pereira
R F
RF
Department of Biology, University of South Carolina, Columbia.
Ortuno
M J
MJ
Lawther
R P
RP
D001426:Bacterial Proteins / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D004926:Escherichia coli / Q000235:genetics*; D034982:Integration Host Factors; D009154:Mutation; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D034982:Integration Host Factors; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI