Photoaffinity labeling of ANF receptor in cultured brain neurones.
A monoiodo derivative of rat atrial natriuretic factor (rANF) was shown to specifically bind to rat brain neurones in culture with low binding site capacity (10-20 fmoles per mg of protein) and high affinity (Kd = 50-100 pM). Several analogs of both rat and human ANF competed with 125I-rANF. No change in the number of binding sites was detected upon morphological differentiation of neurones in vitro. Finally a photoreactive derivative of 125I-rANF was prepared and photoaffinity labeling experiments carried out on cultured neurones. After reduction of disulfide bridges, a single band of Mr 60,000 was specifically labeled whereas without reduction, two labeled components of Mr 60,000 and 117,000 were detected.
Ricard
B
B
Laboratoire de Biochimie et de Biologie Cellulaire, INSERM U 172, CNRS UA 1179, Faculté de Médecine Nord, Marseille, France.
Fourquet
P
P
Massacrier
A
A
Couraud
F
F
D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology*; D002478:Cells, Cultured; D006801:Humans; D008970:Molecular Weight; D009474:Neurons / Q000378:metabolism*; D010777:Photochemistry; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*
D000345:Affinity Labels; D011956:Receptors, Cell Surface; D017461:Receptors, Atrial Natriuretic Factor