Epinephrine-activation of heparin-nonreleasable lipoprotein lipase in 3 skeletal muscle fiber types of the rat.
Epinephrine was used to activate the heparin non-releasable lipoprotein lipase (LPL) in the 3 skeletal muscle fiber types of the perfused rat hindlimb. Following a 9 min washout of the capillary-bound lipoprotein lipase, the hindquarter of the rat was perfused with a buffer containing 10 nM of epinephrine. Activity of the residual LPL in soleus, red vastus lateralis, and white vastus lateralis muscles increased 75%, 96%, and 102% respectively, following epinephrine perfusion. These results suggest that skeletal muscle LPL is under hormonal control possibly through protein phosphorylation by cyclic AMP dependent protein kinase.
Miller
W C
WC
Dept. of Physical Education, Indiana University, Bloomington 47405.
Gorski
J
J
Oscai
L B
LB
Palmer
W K
WK
D000818:Animals; D004789:Enzyme Activation; D004837:Epinephrine / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D006493:Heparin; D008071:Lipoprotein Lipase; D004837:Epinephrine