title: Adenylate cyclase and beta-receptors in salivary glands of rats fed diets containing trans fatty acids.
abstract: The effects of trans fatty acids on adenylate cyclase were determined in the submandibular salivary glands (SMSG) of rats fed diets containing 20% corn oil, 20% partially hydrogenated soybean oil (PHSBO) or 18% PHSBO + 2% corn oil. The fluoride- and isoproterenol-stimulated adenylate cyclase activities were higher in the SMSG from rats fed 20% PHSBO than in the control group fed 20% corn oil. The feeding of 2% corn oil with the diet containing 18% PHSBO resulted in a complete restoration of isoproterenol-stimulated and a partial restoration of fluoride-stimulated adenylate cyclase activity. There was no significant difference in the concentration of the beta-adrenergic receptor or in the receptor-binding affinity constants among the three dietary groups as measured by [3H]dihydroalprenolol (DHA)-binding. Higher fluorescence polarization of diphenyl hexatriene (DPH) was observed in SMSG membranes of rats fed diet containing 20% PHSBO than in the other two oils, suggesting that membrane fluidity may play a role in adenylate cyclase activity.
authors.affiliation: Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70119.
authors.forename: Y F
authors.initials: YF
authors.lastname: Ren
authors.forename: S Q
authors.initials: SQ
authors.lastname: Alam
authors.forename: B S
authors.initials: BS
authors.lastname: Alam
authors.forename: L M
authors.initials: LM
authors.lastname: Keefer
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004041:Dietary Fats / Q000008:administration & dosage*; D005227:Fatty Acids / Q000032:analysis; D005454:Fluorescence Polarization; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012469:Salivary Glands / Q000201:enzymology / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D011943:Receptors, Adrenergic, beta; D000262:Adenylyl Cyclases