title: Pathogenesis of autonomous thyroid nodules: in vitro study of iodine and adenosine 3',5'-monophosphate metabolism.
abstract: The in vitro characteristics of iodide and cAMP metabolism have been compared in tissues from autonomously functioning thyroid nodules and their quiescent counterpart to test the hypothesis that autonomy may result from constitutive activation of the tissue's TSH, cAMP, and protein phosphorylation regulatory axis, as in vivo nodular tissue took up more iodide. This effect was entirely due to increased transport capacity, the affinity of iodide transport, and the fractional binding of iodide to protein remaining unchanged. However, at high concentrations total iodide binding to protein was similar in quiescent and nodular tissue. In both tissues, this metabolic step was enhanced by phorbol esters and the ionophore A23187. As evaluated by autoradiography of two-dimensional gel protein electrophoregrams, no differences in the patterns of protein synthesis or phosphorylation between quiescent and nodular tissue were found. Basal cAMP levels were similar in quiescent and nodular tissue. The cAMP response to TSH was lower in nodular tissue, with no change in sensitivity or kinetics; both tissues responded to forskolin. No systematic suppression of iodide inhibition or abnormal responses to other hormones or neurotransmitters were found. Three proteins (24K-1, 24K-2, and 26K) were phosphorylated only in the presence of TSH or forskolin in both quiescent and nodular tissue. One protein substrate (20K) was phosphorylated in the presence of TSH in the quiescent, but not in the nodular, tissue. In conclusion, 1) slices from autonomous thyroid nodules reproduce the in vivo characteristics of the lesion and are, therefore, a suitable in vitro experimental model for biochemical studies; 2) taken together with data from transplantation experiments, the reproduction in vitro or its in vivo characteristics suggest an inherent defect in the nodule; 3) the homogeneity of biochemical findings within each nodule is compatible with the clonality of the lesion; 4) the autonomous nodule is a minimal deviation tumor; and 5) the characteristics of the TSH, cAMP, protein phosphorylation cascade are qualitatively normal, and autonomy does not result from constitutive activation of this system; and 6) a 20K protein, not phosphorylated in response to TSH in the nodule, could represent an absent negative controlling element.
authors.affiliation: Institute of Interdisciplinary Research, Faculty of Medicine, Université Libre de Bruxelles, Campus Erasme, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Van Sande
authors.forename: F
authors.initials: F
authors.lastname: Lamy
authors.forename: R
authors.initials: R
authors.lastname: Lecocq
authors.forename: N
authors.initials: N
authors.lastname: Mirkine
authors.forename: P
authors.initials: P
authors.lastname: Rocmans
authors.forename: P
authors.initials: P
authors.lastname: Cochaux
authors.forename: J
authors.initials: J
authors.lastname: Mockel
authors.forename: J E
authors.initials: JE
authors.lastname: Dumont
mesh_terms: D000328:Adult; D000001:Calcimycin / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007454:Iodides / Q000493:pharmacokinetics; D007455:Iodine / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008715:Methionine / Q000378:metabolism; D008875:Middle Aged; D010710:Phosphates / Q000378:metabolism; D013959:Thyroid Diseases / Q000473:pathology*; D013961:Thyroid Gland / Q000473:pathology*; D013972:Thyrotropin / Q000494:pharmacology
chemical_list: D007454:Iodides; D010710:Phosphates; D005576:Colforsin; D000001:Calcimycin; D013972:Thyrotropin; D007455:Iodine; D008715:Methionine; D000242:Cyclic AMP