Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.
An alpha 2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet alpha 2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet alpha 2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the alpha 2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the alpha 2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet alpha 2-adrenergic receptor (alpha 2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective alpha-adrenergic ligands.
Regan
J W
JW
Howard Hughes Medical Institute, Duke University Medical Center, Department of Medicine, Durham, NC 27710.
Kobilka
T S
TS
Yang-Feng
T L
TL
Caron
M G
MG
Lefkowitz
R J
RJ
Kobilka
B K
BK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005796:Genes; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011942:Receptors, Adrenergic, alpha / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D011942:Receptors, Adrenergic, alpha; D004247:DNA