Characterization of soluble cyclic AMP phosphodiesterases and partial purification of a major form in human leiomyoma of the uterus.
Human leiomyoma of the uterus contained seven forms of cyclic AMP phosphodiesterase in the crude cytosol as revealed by a specific activity stain on non-denaturing polyacrylamide gel electrophoresis. The enzymes from human myometrium and normal uterus showed an identical activity pattern. Ferguson plot analysis showed four different molecular weight species of Mr 229,000 +/- 4,000, 186,000 +/- 4,000, 174,000 +/- 4,000 and 162,000 +/- 4,000. The Mr 174,000 species comprised four differently charged forms. Sucrose density gradient centrifugation of the crude cytosol revealed the presence of three molecular weight species sedimenting at 11.8S, 8.1S and 3.6S. The Michaelis constant (Km) for the band 1 form which displayed linear kinetics was 5 microM and the band 2 form which produced non-linear kinetics had Km values of 5.8 and 37 microM.
Robinson
M F
MF
Department of Nuclear Medicine, University of the Witwatersrand, Parktown, Johannesburg, South Africa.
Levin
J
J
Savage
N
N
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000145:classification; D007700:Kinetics; D007889:Leiomyoma / Q000201:enzymology*; D008970:Molecular Weight; D014594:Uterine Neoplasms / Q000201:enzymology
D007527:Isoenzymes; D015105:3',5'-Cyclic-AMP Phosphodiesterases