The identification of 5,6-dihydrouridine in normal human urine by combined gas chromatography/mass spectrometry.
The identification of 5,6-dihydrouridine in normal human urine is reported. Partial purification and isolation of the compound by boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography preceded its characterization as a trimethylsilyl derivative by combined gas chromatography/mass spectrometry. Structure proof is based upon a comparison of mass spectral and chromatographic features of the urinary component to that of an authentic reference sample. Additional data derived from high resolution mass measurements and deuterium isotope-labeling experiments provide confirmation of fragment ion structure. The poor detectability inherent in the HPLC/uv analysis of nucleosides is also discussed.
Reimer
M L
ML
Department of Pharmaceutical Sciences, College of Pharmacy, University of Arizona, Tucson 85721.
Schram
K H
KH
Nakano
K
K
Yasaka
T
T
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007553:Isotope Labeling; D008297:Male; D008970:Molecular Weight; D014297:Trimethylsilyl Compounds; D014529:Uridine / Q000031:analogs & derivatives* / Q000652:urine
D014297:Trimethylsilyl Compounds; C010922:5,6-dihydrouridine; D003903:Deuterium; D014529:Uridine