[Effect of UHF and ionizing radiation on the Na-K-ATPase activity of Ehrlich ascitic carcinoma cells].
The levels of Na-K-ATPase were measured in a microsome fraction of Ehrlich ascites carcinoma cells immediately, 10 and 30 min after exposure to microwave (series 1), 7 Gy of gamma-radiation (series 2) and different sequences of complex application of the agents (UHF + gamma-radiation) (series 3), (gamma-radiation + microwave) (series 4). Immediately after exposure, enzyme activity showed a significant decrease in series 1 and 4 (ser. 1-64 and ser. 4-58% as compared with control, alpha less than 0.05). A similar tendency was observed in series 2 and 3 (ser. 2-76 and ser. 3-84% as compared with control, alpha less than 0.05). With further exposure, it remained practically unchanged in ser. 1 and 4; in ser. 2 and 3 it continued to decrease within 10 min (ser. 2-42 and ser. 3-61% as compared with control, alpha less than 0.05); then it increased somewhat (ser. 2-49 and ser. 3-69%, alpha less than 0.05). The most pronounced inactivation was observed after exposure to gamma rays. Application of the latter in conjunction with microwave-field heating was followed by a protective effect.
Smirnov
R V
RV
Andreev
V P
VP
Aleksandrova
R A
RA
Chulkova
G F
GF
Miatliuk
T B
TB
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D005720:Gamma Rays; D066298:In Vitro Techniques; D008872:Microwaves; D011839:Radiation, Ionizing; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D000254:Sodium-Potassium-Exchanging ATPase
Deĭstvie UVCh- i ioniziruiushchego izlucheniĭ na aktivnost' Na-K-ATFazy kletok astsitnoĭ kartsinomy Erlikha.