title: Latent herpes simplex virus type 1 transcripts in peripheral and central nervous system tissues of mice map to similar regions of the viral genome.
abstract: Herpes simplex virus type 1 (HSV-1) DNA and RNA have been detected in peripheral nervous system (PNS) and central nervous system (CNS) tissues of latently infected mice. However, explant methods are successful in reactivating HSV-1 only from latently infected PNS tissues. In this report, latent herpesvirus infections in mouse PNS and CNS tissues were compared by in situ hybridization to determine whether the difference in reactivation was at the level of the virus or the host tissue. It was demonstrated that the HSV-1 transcripts present during latency in the mouse PNS and CNS originated from the same region of the genome, the repeats which bracket the long unique sequence. Therefore, the difference in reactivation with PNS and CNS tissues cannot be accounted for by differences in the extent of the HSV-1 genome transcribed during herpesvirus latency. Latent HSV-1 RNA was detected in the trigeminal ganglia (PNS) and the trigeminal system in the CNS from the mesencephalon to the spinal cord as well as other regions of the CNS not noted previously. Latent HSV-1 RNA was found predominantly in neurons but also in a small number of cells which could not be identified as neuronal cells. It is suggested that host differences in CNS and PNS tissues, rather than differences in latent virus transcription, may be important determinants in the HSV-1 reactivation process in explanted tissues.
authors.affiliation: Wistar Institute, Philadelphia, Pennsylvania 19104-4268.
authors.forename: A M
authors.initials: AM
authors.lastname: Deatly
authors.forename: J G
authors.initials: JG
authors.lastname: Spivack
authors.forename: E
authors.initials: E
authors.lastname: Lavi
authors.forename: D R
authors.initials: DR
authors.lastname: O'Boyle
authors.forename: N W
authors.initials: NW
authors.lastname: Fraser
mesh_terms: D000818:Animals; D001921:Brain / Q000382:microbiology; D001933:Brain Stem / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005814:Genes, Viral; D006561:Herpes Simplex / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009457:Neuroglia / Q000382:microbiology; D009474:Neurons / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification*; D012668:Trigeminal Ganglion / Q000382:microbiology*; D014276:Trigeminal Nerve / Q000382:microbiology*; D014775:Virus Activation
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral