Plasma levels of main granulocyte components during cardiopulmonary bypass.
Plasma levels of granulocyte lactoferrin, myeloperoxidase, and elastase in alpha 1-proteinase inhibitor complex were compared with C3a and C5a levels in 10 patients undergoing cardiopulmonary bypass. At the end of the operation, plasma levels of lactoferrin increased from 97.0 +/- 22.8 to 1257 +/- 139.8 ng/ml, myeloperoxidase increased from 37.1 +/- 4.3 to 170.9 +/- 34.9 ng/ml, and elastase in alpha-proteinase inhibitor complex rose from 89.4 +/- 7.4 to 437.8 +/- 97.3 ng/ml. There was also a significant anaphylatoxin formation. To investigate the relationship between complement and granulocyte activation, patients undergoing cardiopulmonary bypass received the calcium channel blocker nifedipine (orally and intravenously) and the antiplatelet drug dipyridamole. The continuous infusion of nifedipine (5.91 +/- 0.53 micrograms/kg body weight per hour) caused significantly lower levels of elastase in alpha-proteinase inhibitor complex and lactoferrin but not anaphylatoxin. Dipyridamole was without effect on complement and granulocyte activation during cardiopulmonary bypass. Our data demonstrate inhibition of granulocyte activation during cardiopulmonary bypass by continuous infusion of nifedipine, even in the presence of complement activation.
Riegel
W
W
Department of Medicine, University of Freiburg, Federal Republic of Germany.
Spillner
G
G
Schlosser
V
V
Hörl
W H
WH
D000368:Aged; D002315:Cardiopulmonary Bypass; D003176:Complement C3 / Q000378:metabolism; D015926:Complement C3a; D003182:Complement C5 / Q000378:metabolism; D004176:Dipyridamole / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D007781:Lactoferrin / Q000097:blood*; D007782:Lactoglobulins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009543:Nifedipine / Q000627:therapeutic use; D010196:Pancreatic Elastase / Q000097:blood*; D009195:Peroxidase / Q000097:blood*
D003176:Complement C3; D003182:Complement C5; D007782:Lactoglobulins; D004176:Dipyridamole; D015926:Complement C3a; D009195:Peroxidase; D007781:Lactoferrin; D010196:Pancreatic Elastase; D009543:Nifedipine