In situ digestion of Drosophila virilis polytene chromosomes by AluI and HaeIII restriction endonucleases.
Polytene chromosomes of Drosophila virilis were treated with AluI and HaeIII restriction endonucleases. Both enzymes were capable of extensively digesting chromosomal DNA, with the exception of some regions that contain repetitive DNAs. Moreover, a comparison was made between our data and the data already obtained with the same enzymes in D. melanogaster. On this basis, AluI digestion showed that the 5S RNA genes of D. virilis and D. melanogaster have different base composition, while digestion with HaeIII revealed resistance of the histone genes in D. virilis, contrary to what was previously found in D. melanogaster.
Mezzanotte
R
R
Istituto di Biologia Generale, Facoltà di Medicina, Università di Cagliari, Italy.
Bianchi
U
U
Marchi
A
A
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D004330:Drosophila / Q000235:genetics / Q000648:ultrastructure*; D012341:RNA, Ribosomal, 5S / Q000302:isolation & purification
D012341:RNA, Ribosomal, 5S; D004247:DNA; D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases