A 5 kb intergenic region containing ori1 in the mitochondrial DNA of Saccharomyces cerevisiae is dispensable for expression of the respiratory phenotype.
A mutant mitochondrial DNA of Saccharomyces cerevisiae strain R 0.54 has been shown to have a 5 kb deletion which removes the ori1 sequence and its surrounding intergenic sequences between the pro tRNA and 15 S rRNA genes. The deleted DNA has been sequenced and is found to be flanked by two 11 bp direct repeats. The deletion mutant displays a normal phenotype in non-fermentable media. This implies that the intergenic sequence is not required for expression of the respiratory phenotype.
Piskur
J
J
Plant Molecular Biology Group, Research School of Biological Sciences, Australian National University, Canberra.
D001483:Base Sequence; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D010101:Oxygen Consumption; D010641:Phenotype; D012335:RNA, Ribosomal / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012343:RNA, Transfer; D004262:DNA Restriction Enzymes; C024406:endodeoxyribonuclease HpaI; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases