title: Participation of protein kinase C and regulatory G proteins in modulation of the evoked noradrenaline release in brain.
abstract: 1. In the present paper two questions are discussed: (A) Does protein kinase C (PKC) participate in the modulation of evoked noradrenaline release in brain tissue? and (B) Is there any link between presynaptic alpha 2-adrenoceptors and regulatory G proteins? 2. Slices of the middle part of the rabbit hippocampus, labeled with 3H-noradrenaline, were superfused with medium containing the reuptake inhibitor cocaine. During superfusion the tissue was stimulated twice electrically for 2 min each. 3. The PKC activators 4 beta-phorbol 12,13-dibutyrate (4 beta-PDB) and 12-O-tetradecanoyl phorbol 13-acetate (TPA) increased the stimulation-evoked transmitter release in a concentration-dependent manner. 4 alpha-PDB and 4-O-methyl-TPA, which do not activate PKC, were without effect on transmitter release. Polymyxin B, an inhibitor of PKC, diminished the stimulus-evoked overflow and counteracted the effects of the phorbol esters. The increases in release caused by phorbol esters and the alpha 2-adrenoceptor antagonist yohimbine were additive. 4. Treatment of hippocampal tissue with islet-activating protein (IAP) or N-ethylmaleimide (NEM), both known to inactivate the regulatory G proteins Gi and Go by chemical modification, led to a marked increase in evoked noradrenaline release. In addition, the effects of both the alpha 2-adrenoceptor agonist clonidine and the alpha 2-adrenoceptor antagonist yohimbine were inhibited. 5. The facilitatory effects of IAP and NEM on transmitter release were not additive. In synaptosomes prepared from rabbit hippocampus two polypeptides with molecular weights corresponding to those of alpha i and alpha o were 32P-ADP-ribosylated with IAP. Pretreatment of synaptosomes with NEM reduced the subsequent ADP ribosylation by IAP concentration dependently. 6. The above results suggest that PKC is involved in the modulation of noradrenaline release in the rabbit hippocampus. The presynaptic alpha 2-autoreceptors modulate transmitter release by a mechanism which is not directly affected by PKC. The alpha 2-autoreceptor-mediated signals seem to be transduced across the plasma membrane via regulatory G proteins.
authors.affiliation: Institute of Pharmacology, University of Freiburg, Federal Republic of Germany.
authors.forename: G
authors.initials: G
authors.lastname: Hertting
authors.forename: C
authors.initials: C
authors.lastname: Allgaier
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism / Q000502:physiology; D000818:Animals; D004558:Electric Stimulation; D019204:GTP-Binding Proteins / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism* / Q000502:physiology; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology*; D011817:Rabbits; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D010703:Phorbol Esters; D011942:Receptors, Adrenergic, alpha; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D013755:Tetradecanoylphorbol Acetate; D009638:Norepinephrine