Nitrate production and phagocyte activation: differences among Sprague-Dawley, Wistar-Furth and Lewis rats.
We have found that activated resident peritoneal macrophages from Wistar-Furth and Sprague-Dawley rats produce significantly more superoxide (2.2 +/- 0.4 and 3.6 +/- 0.8 nmol of cytochrome c reduced/10(6) cells/10 min respectively) than those from Lewis rats (1.0 +/- 0.3 nmol of cytochrome c reduced/10(6) cells/10 min). Similar results are found in macrophages elicited with intraperitoneal thioglycolate. Furthermore, nitrate excreted in the urine increased greatly when either Wistar-Furth or Sprague-Dawley rats are injected i.p. with iota carrageenan (0.25 g), a response that did not occur with Lewis rats. This strain difference in the manifestations of the inflammatory response correlates with previous observations that Wistar-Furth and Sprague-Dawley rats, but not Lewis rats, develop suture-line colonic tumors when the ureters and bladder base are implanted into the colon.
Dull
B J
BJ
Department of Nutrition (Harvard School of Public Health), Boston, MA 02115.
Gittes
R F
RF
Goldman
P
P
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D003574:Cytochrome c Group / Q000378:metabolism; D005260:Female; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D008297:Male; D009566:Nitrates / Q000652:urine*; D051381:Rats; D011917:Rats, Inbred Lew / Q000502:physiology*; D011919:Rats, Inbred Strains / Q000502:physiology*; D011920:Rats, Inbred WF / Q000502:physiology*; D012737:Sex Factors; D013045:Species Specificity; D013481:Superoxides / Q000378:metabolism; D013864:Thioglycolates / Q000494:pharmacology
D003574:Cytochrome c Group; D009566:Nitrates; D013864:Thioglycolates; D013481:Superoxides; D002351:Carrageenan