Requirement of differentiative signals of both interferon-gamma and 1,25-dihydroxyvitamin D3 for induction and secretion of interleukin-1 by HL-60 cells.
HL-60, a promyelocytic cell line, was treated with both recombinant interferon-gamma (IFN-gamma) and 1,25-(OH)2 vitamin D3 (D3), and the effect on monocyte-specific markers was assessed. IFN-gamma and D3 modulated different stages in the monocytic differentiation with respect to interleukin-1 (IL-1) production and secretion. D3 induced production of an intracellular IL-1 activity that was not secreted after lipopolysaccharide stimulation. IFN-gamma did not induce intracellular IL-1 but differentiated HL-60 cells, which had been treated with D3, so that lipopolysaccharide stimulated IL-1 release. Both D3 and IFN-gamma individually enhanced expression of nonspecific esterase; in combination the two agents potentiated each other. Expression of cell surface Leu-M3 antigen was also enhanced by the combination of these two agents. Thus, IFN-gamma not only potentiated expression of D3-induced markers but also conferred phenotypic properties characteristic of monocytes. IFN-gamma may play a role in the differentiation of bone marrow cells to mature monocytes.
Spear
G T
GT
Department of Human Oncology, University of Wisconsin, Madison 53792.
Paulnock
D M
DM
Helgeson
D O
DO
Borden
E C
EC
D000943:Antigens, Differentiation / Q000032:analysis; D002117:Calcitriol / Q000494:pharmacology*; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D004357:Drug Synergism; D006684:HLA-DR Antigens / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology* / Q000502:physiology; D012822:Silicon Dioxide; D014407:Tumor Cells, Cultured; D001613:beta 2-Microglobulin / Q000378:metabolism
D000943:Antigens, Differentiation; D006684:HLA-DR Antigens; D007375:Interleukin-1; D008070:Lipopolysaccharides; D001613:beta 2-Microglobulin; D012822:Silicon Dioxide; D007371:Interferon-gamma; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D002117:Calcitriol