title: Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells.
abstract: The v-myc oncogene can induce tumours in haematopoietic, mesenchymal and epithelial tissues. The corresponding c-myc proto-oncogene can contribute to the genesis and/or the progression of an equally wide variety of tumours when activated by retroviral insertions, chromosomal translocations or gene amplification. The c-myc gene product is a DNA-binding, nuclear phosphoprotein that is involved in the control of cell proliferation and possibly in DNA synthesis. The replication of Simian virus 40 (SV40) is a useful model system to study eukaryotic DNA replication as the virus relies almost entirely on cellular DNA replication apparatus. The SV40-based vector, pSVEpR4, replicates poorly in the human BJAB lymphoma line and in most human cells, but replicates well in Burkitt lymphoma lines, which have fused immunoglobulin and c-myc genes, resulting in high c-myc expression. Cotransfection of the BJAB cells with a c-myc-expressing construct (pI4-P6) increased the replication of pSVEpR4 tenfold. Our findings indicate that overexpression of the c-myc gene product allows the replication of SV40 in human lymphoma cells, suggesting that c-myc is involved in the control of replication.
authors.affiliation: Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Classon
authors.forename: M
authors.initials: M
authors.lastname: Henriksson
authors.forename: J
authors.initials: J
authors.lastname: Sümegi
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: M L
authors.initials: ML
authors.lastname: Hammarskjöld
authors.forename: M L
authors.initials: ML
authors.lastname: Hammaskjöld
mesh_terms: D000957:Antigens, Viral, Tumor / Q000235:genetics; D002051:Burkitt Lymphoma; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D008223:Lymphoma; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication
chemical_list: D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas