title: Inclusion bodies in recombinant E. coli producing human calcitonin tetramer, as visualized by immuno-gold electron microscopy.
abstract: Inclusion bodies are described in recombinant E. coli cells harboring plasmid for the expression of a synthetic gene coding for human calcitonin tetramer. The inclusion bodies are visualized by electron microscopy and the protein is identified by immuno-gold technique, using antibodies against synthetic human calcitonin. The diameter of the inclusion bodies is 1 micron on the average.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
authors.forename: P
authors.initials: P
authors.lastname: Petrov
authors.forename: E
authors.initials: E
authors.lastname: Jay
authors.forename: I
authors.initials: I
authors.lastname: Ivanov
mesh_terms: D002116:Calcitonin / Q000032:analysis / Q000096:biosynthesis*; D004926:Escherichia coli; D006046:Gold; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000032:analysis* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010957:Plasmids; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; D006046:Gold; D002116:Calcitonin