Distinct tissue specific mitochondrial creatine kinases from chicken brain and striated muscle with a conserved CK framework.
cDNA clones for chicken mitochondrial creatine kinase (Mi-CK) were isolated from a lambda gt11 leg muscle cDNA library and sequenced. The deduced amino acid sequence showed 6 blocks of extensive homologies with the cytosolic creatine kinases and contained twenty N-terminal amino acids, with characteristic features of part of a mitochondrial presequence. The mature enzyme contained 380 amino acids with a calculated Mr of 43'195. RNA hybridization analysis showed corresponding Mi-CK transcripts in cardiac and skeletal muscle, but not in brain RNA. Within the 30 N-terminal amino acids purified brain Mi-CK contained 10 changes with respect to cardiac Mi-CK. Thus multiple isoproteins of mitochondrial creatine kinases of brain and striated muscle are encoded by multiple mRNA's.
Hossle
J P
JP
Institute for Cell Biology, Swiss Federal Institute of Technology, Zurich.
Schlegel
J
J
Wegmann
G
G
Wyss
M
M
Böhlen
P
P
Eppenberger
H M
HM
Wallimann
T
T
Perriard
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002645:Chickens; D003001:Cloning, Molecular; D003402:Creatine Kinase / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D007527:Isoenzymes / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology*; D008931:Mitochondria, Muscle / Q000201:enzymology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012984:Software
D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D003402:Creatine Kinase; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI