Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-sigma 37, E-sigma 32).
The E-sigma 37-transcribed gene ctc was inactivated by a site-specific insertion into the Bacillus subtilis chromosome. The resulting mutation inhibited sporulation by 95% at elevated temperatures (48 degrees C). If the ctc- mutation is placed in a strain that carries a mutation in the closely linked but distinct spoVC gene, ctc now affects both growth and sporulation at elevated temperatures. Growth of the ctc- spoVC285 strain was transiently inhibited when exponentially growing cultures were shifted from 37 degrees C to 48 degrees C. A similar, but less pronounced "growth lag", was also seen in a B. subtilis strain carrying only the spoVC-285 mutation. This finding suggests that both the ctc and spoVC products function in vegetatively growing B. subtilis.
Truitt
C L
CL
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284.
Weaver
E A
EA
Haldenwang
W G
WG
D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D013171:Spores, Bacterial; D013696:Temperature; D014158:Transcription, Genetic
D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes