The accumulation of platelet activating factor in the injured cornea may be interrelated with the synthesis of lipoxygenase products.
The rabbit cornea accumulates platelet activating factor (PAF) three hrs after alkali burn. PAF was isolated by HPLC and assayed by platelet aggregation. This bioactivity was blocked by the PAF receptor antagonists BN 52021 and alprazolam. Added PAF increases the chemiluminescence response of the cornea in vitro and BN 52021 inhibits this effect. In vivo experiments show that the synthesis of 5-HETE and 12-HETE is inhibited by the PAF antagonist BN 52021. It is concluded that a metabolic interrelationship may exist between the PAF cycle and the lipoxygenation of arachidonic acid, and that drugs that affect these lipid mediators may modulate the inflammatory response of the anterior segment of the eye.
Bazan
H E
HE
LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112.
Reddy
S T
ST
Woodland
J M
JM
Bazan
N G
NG
D000525:Alprazolam / Q000494:pharmacology; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002057:Burns, Chemical / Q000378:metabolism*; D003315:Cornea / Q000378:metabolism; D065306:Corneal Injuries; D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D008084:Lipoxygenase / Q000378:metabolism; D016859:Lipoxygenase Inhibitors; D008163:Luminescent Measurements; D010936:Plant Extracts / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000032:analysis* / Q000037:antagonists & inhibitors; D010980:Platelet Membrane Glycoproteins; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000187:drug effects; D043562:Receptors, G-Protein-Coupled
D001095:Arachidonic Acids; D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; C045856:ginkgolide B; D008084:Lipoxygenase; D000525:Alprazolam