Tac expression induced by Epstein-Barr virus is restricted on non-transformable B lymphocytes.
During the course of a comparative study dealing with the immortalization of lymphocytes from a large number of normal healthy donors, we found that B cells of two of these individuals could not be immortalized by Epstein-Barr virus (EBV) under the standard conditions. The expression of the Tac antigen on the membrane of EBV-infected B cells from these two donors was compared with that of B cells from EBV immortalization-susceptible ones. The method used was two-colour immunofluorescence cytofluorometer analysis. We found that the Tac antigen expression was significantly and repeatedly reduced in the case of the two immortalization-resistant donors. This difference might be related to a genetic control of the resistance to EBV-immortalization.
Gosselin
J
J
Centre de Recherche en Immunologie, Institut Armand-Frappier, Ville de Laval, Québec, Canada.
Desrosiers
M
M
Mercier
G
G
Menezes
J
J
Lamoureux
G
G
Oth
D
D
D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D015375:Receptors, Interleukin-2
D007376:Interleukin-2; D011043:Pokeweed Mitogens; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2