title: Construction of cosmid genomic libraries for the normal and myopathic Syrian hamsters.
abstract: Cosmid genomic libraries from both normal and myopathic Syrian hamsters have been constructed. MboI was used to generate 35- to 50-kilobase DNA fragments which were isolated from a 5-25% NaCl gradient. The 35- to 50-kilobase DNA fragments were ligated to the cosmid vector pCV108 and packaged into Escherichia coli DK1. Approximately 3 X 10(5) - 4 X 10(5) clones were obtained per microgram of ligated DNA. Thirteen clones have been isolated from 2 X 10(5) colonies using a cardiac myosin heavy chain clone as a probe. Restriction maps of two of these clones are presented here.
authors.affiliation: Department of Clinical Biochemistry, Banting Institute, University of Toronto, Ont., Canada.
authors.forename: J D
authors.initials: JD
authors.lastname: McCully
authors.forename: M A
authors.initials: MA
authors.lastname: Jandreski
authors.forename: J
authors.initials: J
authors.lastname: Liew
authors.forename: M J
authors.initials: MJ
authors.lastname: Sole
authors.forename: C C
authors.initials: CC
authors.lastname: Liew
mesh_terms: D000818:Animals; D009202:Cardiomyopathies / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae / Q000235:genetics*; D004262:DNA Restriction Enzymes; D008647:Mesocricetus / Q000235:genetics*; D008970:Molecular Weight; D009218:Myosins / Q000235:genetics*
chemical_list: D004262:DNA Restriction Enzymes; D009218:Myosins