An enzymatic assay for picomole levels of phosphatidate.
An assay for phosphatidate that is sensitive, specific, and highly reproducible is described. Phosphatidate is transacylated with methylamine and the glycerol 3-phosphate produced is measured enzymatically. The assay is linear from 50 to 1500 pmol of phosphatidate and can be used to quantitate phosphatidate in small amounts of biological materials.
Bocckino
S B
SB
Howard Hughes Medical Institute, Nashville, Tennessee.
Wilson
P
P
Exton
J H
JH
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D005993:Glycerolphosphate Dehydrogenase; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis / Q000166:cytology; D008297:Male; D008744:Methylamines; D008832:Microchemistry; D009243:NAD; D010712:Phosphatidic Acids / Q000032:analysis*; D010759:Phosphorus Isotopes; D051381:Rats
D008744:Methylamines; D010712:Phosphatidic Acids; D010759:Phosphorus Isotopes; D009243:NAD; C027451:methylamine; D005993:Glycerolphosphate Dehydrogenase; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+)