[The structure of 2 promoters and a transcription terminator from the region of early genes D10-D15 of bacteriophage T5].
The primary structures of two promoters (T5 P31A and T5 P31B) and transcription terminator from the region of bacteriophage T5 early genes D10-D15 were determined. Both promoters (62% of the phage T5 DNA physical map) are located as a tandem at 70 bp distance from each other and are typical for the promoter-consensus structure of DNA of bacteriophage T5 early promoters. The transcription terminator is located in the region of the BamHI site (67% of the phage T5 DNA physical map) and is arranged in good coincidence with the consensus structure of the already known rho-independent terminators of various organisms. The promotor T5 P31A and the terminator were tested for the level of galactokinase synthesis using galK gene-carrying plasmids and D-14C-galactose as a substrate for galactokinase.
Kaliman
A V
AV
Krutilina
A I
AI
Kriukov
V M
VM
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D005686:Galactokinase / Q000235:genetics; D005809:Genes, Regulator; D005814:Genes, Viral; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013604:T-Phages / Q000235:genetics*; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D005686:Galactokinase; D004262:DNA Restriction Enzymes
Struktura dvukh promotorov i terminatora transkriptsii iz oblasti rannikh genov D10-D15 bakteriofaga T5.