title: The effects of structural modifications on the insulin-releasing activity of beta-cell-tropin.
abstract: The minimal effective concentration of the pituitary insulin secretagogue beta-cell-tropin (beta-CT) on the in vitro perfused pancreas was established and the effects of various modifications of the peptide on its potency were tested: iodination with 127I and acetylation reduced the insulin-releasing activity of beta-cell-tropin, and the C-terminal fragments beta-CT-(2-18), beta-CT-(3-18) and beta-CT-(6-18) were all less potent than the intact molecule; beta-CT-(1-6) was not active and did not inhibit beta-CT-induced insulin release.
authors.affiliation: Department of Biochemistry, Imperial College, London, U.K.
authors.forename: S J
authors.initials: SJ
authors.lastname: Dunmore
authors.forename: M
authors.initials: M
authors.lastname: Panico
authors.forename: A T
authors.initials: AT
authors.lastname: Etienne
authors.forename: H R
authors.initials: HR
authors.lastname: Morris
authors.forename: A
authors.initials: A
authors.lastname: Beloff-Chain
mesh_terms: D000107:Acetylation; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D007455:Iodine; D010179:Pancreas / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D007328:Insulin; D010446:Peptide Fragments; C026834:beta-cell tropin; D000324:Adrenocorticotropic Hormone; D007455:Iodine