Conserved and non-conserved regions of the outer coat protein, VP2, of the Australian bluetongue serotype 1 virus, revealed by sequence comparison to the VP2 of North American BTV serotype 10.
The complete nucleotide sequence of the outer coat protein, VP2, of the Australian BTV serotype 1 was determined and found to be 2940 nucleotides in length. An open reading frame of 961 amino acids was found, defining a protein of 112,115 Daltons having a charge of +15 at neutral pH. This coding region was flanked by 5' and 3' non-coding regions of 17 and 37 nucleotides, respectively. When compared to VP2 of the North American BTV serotype 10 (Purdy et al., 1985, J. Virol. 55, 826-830) a homology of 52% at the nucleotide level, and 40% at the amino acid level, was observed. Significant conservation of amino acid sequence was found in eleven distinct regions; the overall hydropathy of the protein, as well as 9 of its 12 cysteine residues, was conserved. The importance of this conservation, in relation to similar observations for the other outer coat protein, VP5, is discussed.
Gould
A R
AR
CSIRO, Australian Animal Health Laboratory, Victoria.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics*; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D012367:RNA, Viral