Production of hepatitis B virus surface antigen particles by the human hepatoma cell line huGK-14 in a serum-free medium.
The human hepatoma cell line, huGK-14, containing integrated hepatitis B virus (HBV) DNAs, produced a large number of 23 nm spherical particles of HBV surface antigen (HBsAg) in a serum-free medium. These particles closely resembled the HBsAg particles found in the sera of HBV carriers and elicited a high antibody response in guinea pigs. The huGK-14 cells in a serum-free medium would provide a new, economic source of HBV vaccine.
Koike
K
K
Department of Gene Research, Japanese Foundation for Cancer Research, Tokyo.
Yoshida
E
E
Katagiri
K
K
Katayanagi
M
M
Oda
M
M
Tsunoo
H
H
Yaginuma
K
K
Kobayashi
M
M
D000917:Antibody Formation; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002460:Cell Line; D002846:Chromatography, Affinity; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology*
D003470:Culture Media; D006514:Hepatitis B Surface Antigens