Purification and properties of a non-histone chromatin protein with a molecular weight of 38,000 daltons specific for transformed rat cells.
Non-histone chromatin proteins prepared from a normal rat cell line (No. 7) and the cells transformed with Rous sarcoma virus (RSV) (s7-1) were compared by means of reverse-phase high performance liquid chromatography (reverse-phase HPLC), followed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The results revealed that several proteins were specifically present in the transformed cell chromatin. A specific non-histone chromatin protein with a molecular weight of 38,000 daltons, 38K protein, was purified as a single species from s7-1 cells. This 38K protein was only detected in the transformed state of the cells transformed with a temperature-sensitive (ts) mutant of the src gene and the mutant cells which showed temperature sensitivity as to the transformation with wild type RSV.
Nakayama
T
T
Department of Biochemistry, Miyazaki Medical College, Japan.
Irikura
M
M
Setoguchi
Y
Y
Matsuo
H
H
Inoue
H
H
Yutsudo
M
M
Hakura
A
A
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D002460:Cell Line; D002472:Cell Transformation, Viral; D002843:Chromatin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D051381:Rats; D012191:Retroviridae Proteins / Q000235:genetics
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D012191:Retroviridae Proteins; D015688:Oncogene Protein pp60(v-src)