title: Enumeration of T and B lymphocytes in bovine leukemia virus-infected cattle, using monoclonal antibodies.
abstract: Monoclonal antibodies and microfluorimetry were used to determine the absolute number of B and T lymphocytes in the blood of bovine leukemia virus (BLV)-infected cows. The blood lymphocyte populations from BLV-infected cows were significantly higher than those from BLV-negative cows. The increase in the lymphocyte population in 3 BLV-infected nonlymphocytotic cows was attributed to a significant increase in the number of T lymphocytes; in 3 BLV-infected persistently lymphocytotic cows, the increase was attributed to a significant increase in the number of B and T lymphocytes. One persistently lymphocytotic cow had a high lymphocyte count, and lymphocytes from this cow contained cells that appeared to stain with markers specific for bovine B and T lymphocytes. We concluded that infection of cattle with the B-cell lymphotropic retrovirus, BLV, not only affected B cells, but also T cells.
authors.affiliation: Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70808.
authors.forename: D L
authors.initials: DL
authors.lastname: Williams
authors.forename: G F
authors.initials: GF
authors.lastname: Amborski
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000276:immunology; D005260:Female; D007938:Leukemia / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine; D007958:Leukocyte Count / Q000662:veterinary; D013601:T-Lymphocytes
chemical_list: D000911:Antibodies, Monoclonal