Infection of undifferentiated and differentiated promyelocytic cells (HL-60) with varicella-zoster virus.
HL-60 cells can be induced to differentiate into macrophage-like cells by treatment with 12-O-tetra-decanoylphorbol-13-acetate (TPA). The relationship between virus replication and cell differentiation was investigated using HL-60 cells that had been induced to differentiate by TPA (dHL-60 cells) and undifferentiated cells (udHL-60 cells). On infection of these cells with cell-free varicella-zoster virus (VZV), virus antigens were detected in dHL-60 cells but not in udHL-60 cells, and the percentage of antigen-positive dHL-60 cells increased during incubation. Similar results were obtained by infectious center assay, and the percentage of antigen-positive cells correlated with the stage of cell differentiation. No significant difference was found in the binding of VZV to dHL-60 cells and udHL-60 cells. Furthermore, trypsin treatment after adsorption suggested that VZV penetrated into udHL-60 cells. These findings indicate that VZV may be able to replicate in mature monocytes but may be harbored in immature monocytes in vivo.
Okuno
T
T
Department of Virology, Osaka University, Japan.
Yamanishi
K
K
Shiraki
K
K
Namazue
J
J
Takahashi
M
M
D000956:Antigens, Viral / Q000032:analysis; D001667:Binding, Competitive; D002454:Cell Differentiation; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D014645:Herpesvirus 3, Human / Q000254:growth & development*; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000382:microbiology*; D011991:Receptors, Virus / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate; D014357:Trypsin; D014407:Tumor Cells, Cultured / Q000382:microbiology*; D010948:Viral Plaque Assay; D014779:Virus Replication
D000956:Antigens, Viral; D011991:Receptors, Virus; D014357:Trypsin; D013755:Tetradecanoylphorbol Acetate