Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii.
The nucleotide sequence of the nifA gene from Azotobacter vinelandii was determined. This gene encodes an Mr = 58,100 polypeptide that shares significant sequence identity when compared to nifA-encoded products from other organisms. Interspecies comparisons of nifA-encoded products reveal that they all have a consensus ATP binding site and a consensus DNA binding site in highly conserved regions of the respective polypeptides. The nifA gene immediately precedes the nifB-nifQ gene region but is unlinked to the major nif gene cluster from A. vinelandii. A potential regulatory gene precedes and is apparently cotranscribed with nifA. Mutant strains that have a deletion or a deletion plus an insertion within nifA are incapable of diazotrophic growth and they fail to accumulate nitrogenase structural gene products.
Bennett
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Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Cannon
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Dean
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D000595:Amino Acid Sequence; D001395:Azotobacter / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000235:genetics; D010957:Plasmids; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009591:Nitrogenase