Provirus integration of bovine leukemia virus into DNA of infected human myeloma cells.
Sixty clones of bovine leukemia virus-infected B-human myeloma ARH77 cells were isolated. The DNAs of all clones were examined for BLV provirus integration by Southern blotting analysis. Proviral sequences were found in DNAs of two clones. One of them (clone I B3) contained one proviral copy with a deletion of approximately 5.5 kb; the other one (clone I F9) carried three integrated proviruses. Viral proteins of 70,000, 42,000 and 35,000 M. W. were found in extracts of clone I F9. In clone I B3 only the 70,000 M.W. protein was detected.
Sláviková
K
K
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Kettmann
R
R
Reinerová
M
M
Zajac
V
V
Portetelle
D
D
Burny
A
A
D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D006801:Humans; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011533:Proviruses / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D014412:Tumor Virus Infections; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics
D004273:DNA, Neoplasm; D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes