[Isolation and properties of the SuaI restriction endonuclease from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius].
A new restriction endonuclease SuaI was isolated from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. The enzyme is an isoschizomer of BspR1; it recognizes tetranucleotide GGCC and cleaves DNA in the center of this sequence. SuaI requires Mg2+, the optimal concentration being 6 mM. KCl at concentrations above 25 mM significantly inhibits the enzyme activity. The pH optimum lies within the range of 6--7 at 70 degrees C, the temperature optimum is at 70--75 degrees C. The enzyme is highly stable at temperatures up to 80 degrees C. DNA of S. acidocaldarius is not cleaved by the enzyme.
Prangishvili
D A
DA
Vashakidze
R P
RP
Chelidze
M G
MG
Chinchaladze
D Z
DZ
Tsalkalamanidze
N V
NV
D001105:Archaea / Q000201:enzymology*; D001419:Bacteria / Q000201:enzymology*; D001483:Base Sequence; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004262:DNA Restriction Enzymes / Q000032:analysis / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D004795:Enzyme Stability; D006358:Hot Temperature; D010957:Plasmids; D013379:Substrate Specificity
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases
Vydelenie i svoĭstva éndonukleazy restriktsii SuaI iz termoatsidofil'noĭ arkhebakterii Sulfolobus acidocaldarius.