Cloning of plasmid genes encoding resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus CH34.
A 238-kilobase-pair plasmid, pMOL30, confers resistance to cadmium, zinc, and cobalt salts in Alcaligenes eutrophus CH34. After Tn5 mutagenesis, restriction nuclease analysis, and Southern DNA-DNA hybridization, a 9.1-kilobase-pair EcoRI fragment was found to harbor all of these resistance properties and was cloned into the broad-host-range hybrid plasmid pRK290. When transferred to a plasmid-free derivative of CH34, the hybrid plasmid conferred the same degree of resistance as the parent plasmid pMOL30. In two other Alcaligenes strains, the hybrid plasmid was expressed, but to a lower degree than in CH34 derivatives.
Nies
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Institut für Pflanzenphysiologie, Zellbiologie und Mikrobiologie, Freien Universität Berlin, Federal Republic of Germany.
Mergeay
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Friedrich
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B
Schlegel
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HG
D000421:Alcaligenes / Q000187:drug effects / Q000235:genetics*; D002104:Cadmium / Q000494:pharmacology*; D003001:Cloning, Molecular; D003035:Cobalt / Q000494:pharmacology*; D003227:Conjugation, Genetic; D003470:Culture Media; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011815:R Factors; D015032:Zinc / Q000494:pharmacology*
D003470:Culture Media; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D002104:Cadmium; D003035:Cobalt; D004262:DNA Restriction Enzymes; D015032:Zinc