title: A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis.
abstract: A series of variants of transposon Tn5 were constructed by replacement of the 2.7-kb central segment which encodes kanamycin resistance with various other resistance-coding genes: tetracycline, chloramphenicol, gentamicin, trimethoprim, streptomycin or ampicillin. A thermosensitive replication mutant of the broad-host-range transmissible plasmid R388 was also constructed for use as a suicide vector for the delivery of transposable elements.
authors.affiliation: Institute of Medical Science, University of Tokyo, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Sasakawa
authors.forename: M
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D005819:Genetic Markers; D014644:Genetic Variation; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics; D009154:Mutation
chemical_list: D004251:DNA Transposable Elements; D005819:Genetic Markers