Loss of gelatinase in elicited peritoneal neutrophils of rats.
In previous studies on the respiratory burst of neutrophils, it was observed that the small, water-soluble quinone, duroquinone, activated the respiratory burst in rat peritoneal neutrophils but not in human blood neutrophils. Here we report studies which indicate that a selective diminution of certain granule enzymes, gelatinase and azocaseinase but not elastase, occurs during elicitation in the rat. Neutrophils circulating in rat blood have about twice the amount of gelatinase and azocaseinase activity as compared to elicited peritoneal cells, and the respiratory burst of circulating cells is stimulated to only about 20% by duroquinone. The significance of these results is considered in terms of activation of the neutrophil respiratory burst.
Morton
M E
ME
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03756.
Schneider
D L
DL
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D003208:Concanavalin A / Q000378:metabolism; D018093:Gelatinases; D006801:Humans; D009504:Neutrophils / Q000201:enzymology*; D010101:Oxygen Consumption; D010434:Pepsin A / Q000097:blood*; D051381:Rats
D003208:Concanavalin A; D010434:Pepsin A; D018093:Gelatinases