High-resolution electron diffraction of reconstituted PhoE porin.
PhoE porin has been reconstituted with phospholipid, forming large membrane patches. Electron diffraction shows that the reconstituted PhoE porin forms highly coherent crystalline arrays, giving structural information to a resolution of 3.4 A. The crystal form is of the orthorhombic space group P2(1)2(1)2, with unit cell dimensions a = 150 A and b = 129 A. Images of negatively stained PhoE crystalline patches show that there are four PhoE porin trimers in a unit cell.
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Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
D001425:Bacterial Outer Membrane Proteins; D003461:Crystallography; D004583:Electrons; D018272:Porins
D001425:Bacterial Outer Membrane Proteins; D018272:Porins