title: Establishment of primate lymphoblastic cell lines by coculture with a simian T-cell leukemia virus-1 positive, hypoxanthine-guanine-phosphoribosyltransferase negative Japanese macaque cell line.
abstract: A cell line (JAMH17+) resistant to 8-azaguanine was established from a human T-cell leukemia virus type 1 related virus (simian T-cell leukemia virus-1) positive Japanese macaque cell line. Lymphoblastic cell lines were established from the peripheral blood mononuclear cells of humans, hominoids, and several species of macaques by coculture with JAMH17+ in hypoxanthine-aminopterin-thymidine medium. HTLV-1 specific antigen was detected in some of the established cell lines. Phenotypic analysis showed that several cell lines of crab-eating macaques expressed Leu11a antigen, which is a marker of human natural killer cells.
authors.affiliation: Primate Research Institute, Kyoto University, Aichi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Murayama
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: M
authors.initials: M
authors.lastname: Minezawa
authors.forename: A
authors.initials: A
authors.lastname: Noguchi
authors.forename: O
authors.initials: O
authors.lastname: Takenaka
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D017977:Deltaretrovirus / Q000276:immunology; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes; D008251:Macaca; D002679:Pan troglodytes; D010641:Phenotype; D011148:Pongo pygmaeus; D012190:Retroviridae / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000956:Antigens, Viral