title: Presence of functional receptors for atrial natriuretic peptide in human pheochromocytoma.
abstract: Pheochromocytoma, a catecholamine-secreting adrenomedullary tumor, has been shown to contain the functional receptor for human atrial natriuretic peptide(h-ANP). Release of catecholamines from tissue slices of pheochromocytoma was inhibited by h-ANP in a dose-dependent manner. Binding assays using 125I-ANP revealed a single class of high affinity binding sites for ANP. When covalently tagged with 125I-ANP and electrophoresed under non-reducing and reducing conditions, the receptor migrated as a 140-kDa band and a 70-kDa band, respectively, reflecting its disulfide-linked subunit structure. The presence of ANP receptor in pheochromocytoma was further demonstrated by immunohistochemistry; the tumor was positively stained with an antireceptor antiserum. The antiserum was also useful to establish the zona glomerulosa localization of ANP receptor in the normal human adrenal gland.
authors.affiliation: Second Department of Internal Medicine, Yokohama City University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shionoiri
authors.forename: N
authors.initials: N
authors.lastname: Hirawa
authors.forename: I
authors.initials: I
authors.lastname: Takasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: K
authors.initials: K
authors.lastname: Minamisawa
authors.forename: E
authors.initials: E
authors.lastname: Miyajima
authors.forename: Y
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: K
authors.initials: K
authors.lastname: Shimoyama
authors.forename: M
authors.initials: M
authors.lastname: Shimonaka
authors.forename: M
authors.initials: M
authors.lastname: Ishido
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology; D004837:Epinephrine / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009638:Norepinephrine / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism* / Q000473:pathology; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D009638:Norepinephrine; D004837:Epinephrine