Physicochemical studies of binding of 4-methylumbelliferyl beta-D-galactopyranoside to cold agglutinin.
The fluorescence of 4-methylumbelliferyl beta-D-galactopyranoside (MeUmbGalp) was quenched in the presence of cold agglutinin, showing that there was binding between MeUmbGalp and cold agglutinin. That binding was saccharide-specific. By using this quenching phenomenon, the association constants (Ka) of the binding of cold agglutinin at different temperatures (10 degrees C and 15 degrees C) to MeUmbGalp and also the number of binding sites were calculated. The Ka values were found to be 2.63 x 10(3) M-1 at 10 degrees C and 1.58 x 10(3) M-1 at 15 degrees C. Though there is a change in Ka values, the number of binding sites was calculated to be six at both temperatures (10 degrees C and 15 degrees C). From the Ka values the thermodynamic parameters (free energy, enthalpy and entropy) of the binding were derived, and analysis of the data indicated that the binding is spontaneous, exothermic and hydrophobic in nature.
Mitra
D
D
Indian Institute of Chemical Biology, Calcutta, India.
Sarkar
M
M
D000373:Agglutinins; D001665:Binding Sites; D003450:Cryoglobulins; D005456:Fluorescent Dyes; D005697:Galactosides; D006027:Glycosides; D006923:Hymecromone / Q000031:analogs & derivatives; D008433:Mathematics; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014468:Umbelliferones / Q000031:analogs & derivatives
D000373:Agglutinins; D003450:Cryoglobulins; D005456:Fluorescent Dyes; D005697:Galactosides; D006027:Glycosides; D014468:Umbelliferones; C029443:cold agglutinins; D006923:Hymecromone; C026845:4-methylumbelliferyl-galactopyranoside