title: Gastric cell c-AMP stimulating autoantibodies in duodenal ulcer disease.
abstract: Gastric cell c-AMP stimulating antibodies (GCS-Ab) were studied in 30 patients with duodenal ulcer (DU) disease. Semipurified immunoglobulin (Ig) preparations from 13/30 patients stimulated c-AMP production in parietal cell enriched gastric cell suspensions obtained from male guinea pig stomachs. Maximum stimulation (varying between 260 and 547%) was reached after four hours incubation with 2 and 4 mg/ml Ig concentrations. The 13 patients with gastric cell stimulating antibodies (GCS-Ab), all male patients, developed the disease at a younger age (nine of 13 under the age of 30), had a longer duration of symptoms (mean 18.4 years), and had a higher incidence of DU in their families (61%). Eight of 13 (61%) in the GCS-Ab+ group did not respond to anti-H2-R drugs, whereas in the negative patients only three of 17 (18%) were classified as 'non-responders'. Remarkably few conventional autoantibodies were detected in our series. Gastric cell stimulating antibodies are a new addition to the growing list of receptor antibodies in human diseases and the described in vitro test should provide an easier tool for screening large populations.
authors.affiliation: Department of Immunology, Middlesex Hospital Medical School, London.
authors.forename: F
authors.initials: F
authors.lastname: De Lazzari
authors.forename: R
authors.initials: R
authors.lastname: Mirakian
authors.forename: L
authors.initials: L
authors.lastname: Hammond
authors.forename: C
authors.initials: C
authors.lastname: Venturi
authors.forename: R
authors.initials: R
authors.lastname: Naccarato
authors.forename: G F
authors.initials: GF
authors.lastname: Bottazzo
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001681:Biological Assay; D000242:Cyclic AMP / Q000032:analysis*; D004306:Dose-Response Relationship, Immunologic; D004381:Duodenal Ulcer / Q000276:immunology*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D008875:Middle Aged; D010295:Parietal Cells, Gastric / Q000378:metabolism
chemical_list: D001323:Autoantibodies; D000242:Cyclic AMP