Insulin mRNA content in pancreatic beta cells of coxsackievirus B4-induced diabetic mice.
Molecular hybridization was used to measure poly(A)-containing mRNA and insulin mRNA, and to evaluate viral persistence, in pancreatic beta cells of coxsackievirus B4-induced diabetic mice. Cellular RNA was hybridized with [3H]poly(U) to measure poly(A)-containing total mRNA, 32P-labeled preproinsulin I and II probes to measure insulin mRNA, and a 32P-labeled virus-specific probe to evaluate persistence. The infected mice (80-90%) showed subnormal blood glucose at 72 h postinfection and were hyperglycemic at 6 and 8 weeks. Poly(A)-containing total mRNA decreased by about 26% at 72 h and 6 weeks and by 49% at 8 weeks, while preproinsulin I mRNA by 30% and preproinsulin II by 46% at 8 weeks postinfection compared to control. Viral sequences were abundant at 72 h and in fair amounts later. It appears that persistent viral infection produces a pathological state, which impairs beta cell function to reduce insulin mRNA and consequently insulin synthesis apparently leading to hyperglycemia.
Chatterjee
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NK
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
Nejman
C
C
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003001:Cloning, Molecular; D003384:Coxsackievirus Infections / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000382:microbiology*; D029822:Enterovirus B, Human / Q000235:genetics; D007328:Insulin / Q000235:genetics*; D007515:Islets of Langerhans / Q000378:metabolism / Q000382:microbiology*; D007700:Kinetics; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000302:isolation & purification
D001786:Blood Glucose; D007328:Insulin; D012333:RNA, Messenger; D012367:RNA, Viral