Comparison of the generation in vitro of chemotactically active LTB4 and its omega-metabolites by human neutrophils and lymphocytes/monocytes.
To assess the relative contribution of different leucocyte subpopulations to LTB4 production, peripheral blood leucocytes from human donors were separated into polymorphonuclear neutrophils (PMN) and lymphocytes/monocytes (L/M) and were then stimulated in vitro with the Ca-ionophore A 23187 for different times. The supernatants were analysed for their contents of leukotriene B4 (LTB4) and its omega-metabolites by HPLC-analysis and column fractions were also examined for their chemotactic activities towards eosinophils in vitro. PMN supernatants contained greater quantities of LTB4, 20-OH-LTB4, 20-COOH-LTB4, and chemotactic activities than did L/M supernatants. On the other hand, the time dependent decrease of LTB4 and chemotactic activity and the increase of omega-metabolites were higher in PMN than in L/M. These results would correlate with the greater role of PMN in acute and that of monocytes in chronic inflammation.
Rosenbach
T
T
University Clinics, Department of Dermatology, Münster, FRG.
Czarnetzki
B M
BM
D000001:Calcimycin / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000097:blood*; D007975:Leukotriene B4 / Q000096:biosynthesis* / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D013997:Time Factors
D007975:Leukotriene B4; D000001:Calcimycin