Evaluation of three methods for typing herpes simplex virus.
Three methods of typing herpes simplex virus were compared. A total of 111 clinical isolates obtained from patients not treated with antivirals and seven resistant mutants selected in vitro were tested by immunofluorescence assay using antibodies against herpes simplex virus types 1 and 2. Twenty-nine isolates were also studied by restriction endonuclease analysis. The sensitivity of isolates and resistant mutants to (E)-5-(2-bromovinyl)-2'-deoxyuridine was determined. Although a clear difference between the 50% inhibitory dose for type 1 and type 2 isolates was observed, some drug-resistant mutants might be misidentified by this method.
Salmerón
F
F
Centro Nacional de Farmacobiología, Carretera de Majadahonda a Pozuelo, Madrid, Spain.
Pérez Breña
P
P
Rojas
J M
JM
Hernáez Lobo
R
R
Tabarés
E
E
D000818:Animals; D000911:Antibodies, Monoclonal; D000998:Antiviral Agents / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D008826:Microbial Sensitivity Tests; D018139:Simplexvirus / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D004279:DNA, Viral; C020235:brivudine; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases; D001973:Bromodeoxyuridine