Localization of sodium-potassium adenosine triphosphatase in sheep myocardium by immunoelectron microscopy.
Immunohistochemical localization of sodium and potassium dependent adenosine triphosphatase (Na,K-ATPase) employing polyclonal antibodies was carried out on sheep myocardium. The tissue was fixed with glutaraldehyde or a paraformaldehyde/lysine/periodate combination fixative and embedded in different embedding media. Ultrathin sections were labeled with rabbit anti-sheep Na,K-ATPase antiserum followed by sheep anti-rabbit immunoglobulin complexed with colloidal gold. Glycol methacrylate embedded tissue provided good structural preservation and showed specific immunohistochemical labeling. Analysis of the gold particle distribution showed a significantly higher number of Na,K-ATPase immunoreactive sites associated with cell membrane and T-tubules.
Kossenjans
W
W
Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Ohio 45267.
Ashraf
M
M
D000818:Animals; D006046:Gold; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D012756:Sheep; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*
D006046:Gold; D000254:Sodium-Potassium-Exchanging ATPase