A dot-immunobinding assay for infectious bronchitis virus.
Common Whatman filter paper grade 1 and nitrocellulose membrane were compared for their sensitivity in a dot-immunobinding assay for detection of serum antibody titers to Arkansas avian infectious bronchitis virus (AIBV). For a blue to purple color detection, serum antibodies were bound to AIBV antigen adsorbed on the filter-paper discs or nitrocellulose membrane. Rabbit anti-chicken IgG horseradish-peroxidase (HRP) conjugate and hydrogen peroxide with 4-chloro-1-naphthol (HRP-color development reagent) were applied. The study indicates that very small amounts of antigen/antisera are needed for the dot-immunobinding assay. The test is sensitive, economical, and easy to run and can be completed within 6-8 hours.
Muneer
M A
MA
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Newman
J A
JA
Sivanandan
V
V
Halvorson
D A
DA
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens / Q000276:immunology*; D003101:Collodion; D003332:Coronaviridae / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007118:Immunoassay / Q000379:methods; D001351:Infectious bronchitis virus / Q000276:immunology*; D010209:Paper
D000914:Antibodies, Viral; D003101:Collodion