title: Cloning and nucleotide sequencing of phenylalanine dehydrogenase gene of Bacillus sphaericus.
abstract: The gene coding for phenylalanine dehydrogenase [PDH; L-phenylalanine: NAD+ oxidoreductase (deaminating); EC 1.4.1.-] from Bacillus sphaericus SCRC-79a was cloned onto plasmid pUC9, and the nucleotide sequence of the 2-kb DNA region of the insert was determined. A 1143-bp open reading frame consisting of 381 codons was identified as a pdh gene coding for PDH.
authors.affiliation: Sagami Chemical Research Center, Kanagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Okazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Hibino
authors.forename: Y
authors.initials: Y
authors.lastname: Asano
authors.forename: M
authors.initials: M
authors.lastname: Ohmori
authors.forename: N
authors.initials: N
authors.lastname: Numao
authors.forename: K
authors.initials: K
authors.lastname: Kondo
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010957:Plasmids
chemical_list: D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase; D004262:DNA Restriction Enzymes