Calmodulin modulates prolactin secretion in vitro: studies with calmodulin containing liposomes.
The control of prolactin secretion by Ca calmodulin and cyclic AMP was studied. Ca++ ionophore A23187 stimulated both cyclic AMP accumulation and prolactin release by primary culture of anterior pituitary cells in vitro. The increase of cyclic AMP formation by A23187 preceded that of prolactin release. To test the calmodulin involvement in these processes we used either selective calmodulin antagonist, the naphthalene sulphonamide derivative W7, or calmodulin containing liposomes. W7 dose dependently inhibited both basal or A23187 stimulated cyclic AMP accumulation and prolactin secretion. Insertion of Ca calmodulin within the cells stimulated prolactin secretion without modifying cyclic AMP accumulation. W7 inhibited the Ca calmodulin containing liposomes stimulation of prolactin release. These results suggest that calmodulin participates to the process of prolactin release.
Schettini
G
G
Department of Pharmacology, II School of Medicine, University of Naples, Italy.
Florio
T
T
Meucci
O
O
Landolfi
E
E
Cronin
M J
MJ
MacLeod
R M
RM
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000502:physiology; D007700:Kinetics; D008081:Liposomes / Q000008:administration & dosage*; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D051381:Rats
D002147:Calmodulin; D008081:Liposomes; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D000001:Calcimycin; D011388:Prolactin; D000242:Cyclic AMP; D002118:Calcium