Decrease in the c-myb gene transcript during differentiation of mouse teratocarcinoma stem cells.
Transcript of the c-myb gene, one of the proto-oncogenes, was clearly detected in undifferentiated mouse F9 teratocarcinoma stem cells, but not in terminally differentiated mouse parietal endoderm PYS-2 cells. As F9 cells can be induced to differentiate into parietal endoderm-like cells by the addition of retinoic acid and dibutyryl cAMP, we examined levels of the c-myb transcript under this experimental condition and found that the c-myb transcript was decreased significantly. Thus, a decrease in the c-myb transcript is probably related to early differentiation of mammalian cells.
Fukuda
M
M
Department of Biochemistry, Kumamoto University Medical School, Japan.
Ikuma
S
S
Setoyama
C
C
Shimada
K
K
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis; D054278:Embryonal Carcinoma Stem Cells; D051379:Mice; D014411:Neoplastic Stem Cells / Q000032:analysis*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D013724:Teratoma / Q000032:analysis / Q000235:genetics*; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D014212:Tretinoin; D003994:Bucladesine