title: The pentafunctional arom enzyme of Saccharomyces cerevisiae is a mosaic of monofunctional domains.
abstract: The nucleotide sequence of the Saccharomyces cerevisiae ARO1 gene which encodes the arom multifunctional enzyme has been determined. The protein sequence deduced for the pentafunctional arom polypeptide is 1588 amino acids in length and has a calculated Mr of 174555. Functional regions within the polypeptide chain have been identified by comparison with the sequences of the five monofunctional Escherichia coli enzymes whose activities correspond with those of the arom multifunctional enzyme. The observed homologies demonstrate that the arom polypeptide is a mosaic of functional domains and are consistent with the hypothesis that the ARO1 gene evolved by the linking of ancestral E. coli-like genes.
authors.affiliation: Department of Biochemistry, University of Glasgow, U.K.
authors.forename: K
authors.initials: K
authors.lastname: Duncan
authors.forename: R M
authors.initials: RM
authors.lastname: Edwards
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006836:Hydro-Lyases / Q000235:genetics; D008190:Lyases / Q000235:genetics; D008969:Molecular Sequence Data; D009030:Mosaicism; D009097:Multienzyme Complexes / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor); D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014166:Transferases
chemical_list: D003062:Codon; D009097:Multienzyme Complexes; C019728:arom enzyme; D000429:Alcohol Oxidoreductases; D014166:Transferases; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); D008190:Lyases; D006836:Hydro-Lyases