Characterization of novel anti-tumor effector cells in long-term cultures of human tumor-infiltrating lymphocytes.
TIL were isolated from human solid tumors and cultured in rIL 2. Long-term TIL lines from primary tumors showed a shorter lag period, better expansion, and higher anti-tumor activity in vitro than those from metastatic tumors. The anti-tumor effector cells in TIL and A-PBL cultures had CD3+ Leu 19+ and CD3- Leu19+ phenotypes and morphology consistent with LGL/LAK cells. These cells mediated lysis of both autologous and allogeneic fresh tumor cell targets, while CD3+ Leu19- cells had little or no antitumor cytotoxicity.
Whiteside
T L
TL
Department of Pathology, University of Pittsburgh, PA.
Heo
D S
DS
Takagi
S
S
Herberman
R B
RB
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000276:immunology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D007376:Interleukin-2 / Q000494:pharmacology; D008113:Liver Neoplasms; D008214:Lymphocytes / Q000276:immunology*; D009369:Neoplasms / Q000276:immunology*; D013997:Time Factors
D000945:Antigens, Differentiation, T-Lymphocyte; D007376:Interleukin-2