title: Analysis of the TNF receptor on KYM cells by binding assay and affinity cross-linking.
abstract: The existence of a tumor necrosis factor (TNF) receptor on KYM human myosarcoma cells was elucidated by Scatchard plot and cross-linking analysis. The average number of specific binding sites per cell was 15,300 and the apparent dissociation constant Kd was 4.0 X 10(-10) M. The estimated relative molecular mass of a TNF receptor on KYM cell was approximately 95,000.
authors.affiliation: Department of Internal Medicine, Sapporo Medical College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Niitsu
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Sone
authors.forename: H
authors.initials: H
authors.lastname: Neda
authors.forename: N
authors.initials: N
authors.lastname: Yamauchi
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: I
authors.initials: I
authors.lastname: Urushizaki
mesh_terms: D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D009217:Myosarcoma / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis*; D018124:Receptors, Tumor Necrosis Factor; D012509:Sarcoma / Q000032:analysis*
chemical_list: D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor