Cooperation between the polyomavirus middle-T-antigen gene and the human c-myc oncogene in a rat thyroid epithelial differentiated cell line: model of in vitro progression.
Two rat thyroid epithelial differentiated cell lines, PC Cl 3 and PC myc, were infected with the polyoma murine leukemia virus (PyMLV) carrying the Middle-T-antigen gene of polyomavirus. After infection, both cell lines acquired the typical markers of neoplastic transformation; however, the PC myc cells showed a greater malignant phenotype. Furthermore, the thyroid differentiated functions were completely suppressed in PC myc cells transformed by PyMLV, whereas they were, at least partially, retained in PC Cl 3 cells transformed by PyMLV, and in particular, thyroglobulin synthesis and secretion were not affected at all. Since no differences in the expression of the middle-T-antigen gene were observed in the two PyMLV-transformed cell lines, the different properties shown by these two infected cell lines must be ascribed to the expression of the c-myc oncogene.
Berlingieri
M T
MT
Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Portella
G
G
Grieco
M
M
Santoro
M
M
Fusco
A
A
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000502:physiology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004848:Epithelium; D005814:Genes, Viral; D006801:Humans; D009857:Oncogenes; D011120:Polyomavirus / Q000235:genetics / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D016271:Proto-Oncogene Proteins c-myc; D051381:Rats; D011916:Rats, Inbred F344; D013954:Thyroglobulin / Q000378:metabolism; D013961:Thyroid Gland
D000952:Antigens, Polyomavirus Transforming; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D013954:Thyroglobulin