title: The primary structure of chicken muscle acylphosphatase isozyme Ch2.
abstract: The amino acid sequence of one, Ch2, of the two isozymes of chicken muscle acylphosphatase was determined. It consists of 98 amino acid residues with N-acetylalanine at the amino(N)-terminus and contains no cysteine: Ac-Ala-Gly-Ser-Glu- Gly-Leu-Met-Ser-Val-Asp-Tyr-Glu-Val-Ser-Gly-Arg-Val-Gln-Gly-Val-Phe-Phe- Arg- Lys-Tyr-Thr-Gln-Ser-Glu-Ala-Lys-Arg-Leu-Gly-Leu-Val-Gly-Trp-Val-Arg-Asn- Thr- Ser-His-Gly-Thr-Val-Gln-Gly-Gln-Ala-Gln-Gly-Pro-Ala-Ala-Arg-Val-Arg-Glu- Leu- Gln-Glu-Trp-Leu-Arg-Lys-Ile-Gly-Ser-Pro-Gln-Ser-Arg-Ile-Ser-Arg-Ala-Glu- Phe- Thr-Asn-Glu-Lys-Glu-Ile-Ala-Ala-Leu-Glu-His-Thr-Asp-Phe-Gln-Ile-Arg-Lys- COOH. The sequence differs in 44% of the total positions from the other isozyme, Ch1. Comparison of the sequence and the predicted conformational profile of Ch2 with those of Ch1 suggests that they share a common evolutionary origin and appear to have retained similar conformations throughout their evolutionary development.
authors.affiliation: Institute of Immunological Science, Hokkaido University.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohba
authors.forename: O
authors.initials: O
authors.lastname: Minowa
authors.forename: Y
authors.initials: Y
authors.lastname: Mizuno
authors.forename: H
authors.initials: H
authors.lastname: Shiokawa
mesh_terms: D017766:Acid Anhydride Hydrolases; D000421:Alcaligenes / Q000201:enzymology; D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D002645:Chickens; D003488:Cyanogen Bromide; D007527:Isoenzymes; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D010744:Phosphoric Monoester Hydrolases; D011487:Protein Conformation; D012697:Serine Endopeptidases; D000097024:Acylphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D003488:Cyanogen Bromide; D007527:Isoenzymes; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D010744:Phosphoric Monoester Hydrolases; D012697:Serine Endopeptidases; D000097024:Acylphosphatase; C038220:acylaminoacyl-peptidase; C028344:glutamyl endopeptidase; C036813:lysyl endopeptidase