Analysis of human papillomavirus type 16 polypeptides in transformed primary cells.
The close association between human papillomavirus type 16 and cervical cancer implies some role for the virus in the development of this cancer. It has been demonstrated that HPV-16 DNA sequences in the presence of activated ras are capable of transforming primary baby rat kidney cells. This communication describes the characterization of these transformed cells. All cell lines are shown to be tumorigenic in immunocompetent rats and there is continued expression of the HPV-16 E6 and E7 polypeptides in a number of the cell lines analyzed, suggesting a role for at least one of these proteins in the maintenance of the transformed phenotype.
Banks
L
L
Molecular Virology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Crawford
L
L
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D011905:Genes, ras; D007668:Kidney; D009374:Neoplasms, Experimental / Q000209:etiology; D009856:Oncogene Proteins, Viral / Q000032:analysis / Q000502:physiology; D027383:Papillomaviridae / Q000032:analysis*; D011518:Proto-Oncogene Proteins / Q000502:physiology; D016283:Proto-Oncogene Proteins p21(ras); D051381:Rats; D011994:Recombinant Proteins / Q000032:analysis; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis*
D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D016283:Proto-Oncogene Proteins p21(ras)