Specific interactions between transcription factors and the promoter-regulatory region of the human cytomegalovirus major immediate-early gene.
Repeat sequence motifs as well as unique sequences between nucleotides -150 and -22 of the human cytomegalovirus immediate-early 1 gene interact in vitro with nuclear proteins. We show that a transcriptional element between nucleotides -91 and -65 stimulated promoter activity in vivo and in vitro by binding specific cellular transcription factors. Finally, a common sequence motif, (T)TGG/AC, present in 15 of the determined binding sites suggests a particular class of nuclear factors associated with the immediate-early 1 gene.
Ghazal
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P
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Lubon
H
H
Hennighausen
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L
D001483:Base Sequence; D001665:Binding Sites; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D015696:Gene Products, tat; D005809:Genes, Regulator; D005814:Genes, Viral; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism*
D004279:DNA, Viral; D015696:Gene Products, tat; D014157:Transcription Factors