Effects of estrogen antagonists on estradiol-enhanced radiation transformation in vitro.
We have previously reported that radiation and 17 beta-estradiol can induce transformation in vitro in C3H 10T1/2 cells. In the present series of experiments, we have observed that antagonists of estrogen action, such as c-AMP activating agents (theophylline and dibutyryl c-AMP) and the anti-estrogen, tamoxifen, suppress radiation/17 beta-estradiol enhanced transformation in vitro. None of these known estrogen antagonists had a significant effect on transformation induced by radiation alone. Our results with added dibutyryl c-AMP, theophylline and tamoxifen suggest that estrogen receptor complex formation may play a role in estrogen-enhanced radiation transformation in vitro.
Umans
R S
RS
Laboratory of Radiobiology, Harvard School of Public Health, Boston, MA 02115.
Kennedy
A R
AR
D003994:Bucladesine / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000528:radiation effects*; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000378:metabolism
D004965:Estrogen Antagonists; D011960:Receptors, Estrogen; D004958:Estradiol; D003994:Bucladesine