Immunological relationships between malignant catarrhal fever virus (alcelaphine herpesvirus 1) and bovine cytomegalovirus (bovine herpesvirus 3).
Strains of malignant catarrhal fever virus (alcelaphine herpesvirus 1 (AHV-1)) and bovine cytomegalovirus (bovine herpesvirus 3 (BHV-3)) were compared for serological relatedness by cross-titration in an indirect immunofluorescent (IIF) antibody assay. There was definite cross-reactivity between these 2 viruses, with heterologous sera staining intracellular and membrane antigens of infected cells. Heterologous antibody titres were approximately 50-fold lower than homologous titres and could be removed by absorption with either homologous or heterologous virus-infected cells, but not with uninfected cells. Regression analyses of IIF antibody titres to AHV-1 and BHV-3 virus in 3 groups of wild ungulate sera also indicated a serological relationship between these herpesviruses. In a cross-immunity trial, 2 of 3 cattle immunized with a BHV-3 virus and 2 of 3 cattle immunized with avirulent AHV-1 resisted challenge with virulent AHV-1-infected blood which killed 3 unimmunized controls. These results are discussed particularly with respect to the involvement of BHV-3 in malignant catarrhal fever.
Rossiter
P B
PB
Division of Virology, Kenya Agricultural Research Institute, Kikuyu.
Gumm
I D
ID
Mirangi
P K
PK
D000818:Animals; D000866:Antelopes; D000914:Antibodies, Viral / Q000032:analysis*; D002020:Buffaloes; D002417:Cattle; D002460:Cell Line; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D008304:Malignant Catarrh / Q000382:microbiology / Q000517:prevention & control; D009500:Neutralization Tests; D012044:Regression Analysis; D014709:Vero Cells
D000914:Antibodies, Viral