Cloning of the Rhodobacter capsulatus hemA gene.
Portions of the Rhodobacter capsulatus hemA gene have been cloned from a hemA::Tn5 insertion strain into the lambda bacteriophage derivative EMBL3. A cosmid containing the wild-type R. capsulatus hemA gene was isolated by complementation of the hemA::Tn5 mutant. The cosmid contains a 1.4-kilobase EcoRI fragment that spans the hemA::Tn5 insertion site. The entire hemA gene is contained in this fragment and the adjacent 0.6-kilobase EcoRI fragment.
Biel
S W
SW
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
Wright
M S
MS
Biel
A J
AJ
D000624:5-Aminolevulinate Synthetase / Q000235:genetics*; D000622:Aminolevulinic Acid / Q000096:biosynthesis; D010582:Bacteriophage lambda; D003001:Cloning, Molecular; D003360:Cosmids; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009693:Nucleic Acid Hybridization; D012241:Rhodopseudomonas / Q000201:enzymology / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D000622:Aminolevulinic Acid; D000624:5-Aminolevulinate Synthetase; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI