title: Increased permissivity of monkey cells to human adenovirus multiplication is affected by culturing conditions and correlates with both synthesis of virion fiber protein and altered splicing of its mRNA.
abstract: Human adenoviruses (Ad2) grow poorly in CV-1 (monkey) cells, resulting in an abortive infection. During abortive infections synthesis of the fiber protein is approximately 100-fold depressed compared to its synthesis in CV-1 cells productively infected with a host range mutant of adenovirus (Ad2hr400), while the corresponding mRNA is reduced only 10-fold. We found that passage of CV-1 cells under modified culture conditions resulted in loss of restrictiveness for Ad2 growth. As cells became more permissive, fiber polypeptide synthesis was enhanced although fiber mRNA levels did not necessarily increase. Analysis of the 5' ends of fiber message isolated from cells in various stages of permissivity showed a direct correlation between efficient synthesis of the fiber protein and an altered splicing pattern of the fiber message.
authors.affiliation: Waksman Institute, Rutgers State University of New Jersey, Piscataway 08855.
authors.forename: L
authors.initials: L
authors.lastname: Silverman
authors.forename: V
authors.initials: V
authors.lastname: Cleghon
authors.forename: D F
authors.initials: DF
authors.lastname: Klessig
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics; D036022:Capsid Proteins; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D011233:Precipitin Tests; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis
chemical_list: D036022:Capsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; C018315:hexon capsid protein, Adenovirus