title: Role of RAV-0 genes in the permissive replication of subgroup E avian leukosis viruses on line 15Bev1 CEF.
abstract: Rous associated virus type-0 (RAV-0) is a replication-competent endogenous virus of chickens which grows more efficiently on chick embryo fibroblasts (CEFs) from line 15B chickens than on CEFs from line K28. Differences in viral growth on these two sources of cells have been attributed to an early event in the retrovirus life-cycle, at or before viral DNA synthesis. Five in vitro constructed avian leukosis viruses (ALVs) as well as RAV-0 (a subgroup E ALV), RAV-1 (subgroup A), and RAV-2 (subgroup B) have been assessed for their relative growth on 15Bev1 and K28 CEFs. More efficient replication on 15Bev1 CEFs than on K28 CEFs was determined by subgroup E-encoding sequences in env. Subgroup A and B envelope sequences as well as viral LTR, gag, and pol sequences did not obviously bias relative rates of viral replication on the two cell types. We suggest that the unusually permissive replication of subgroup E viruses on 15B CEFs is a receptor-mediated phenomenon and that the line 15B receptor for subgroup E ALVs is more efficient than that of line K28.
authors.affiliation: Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
authors.forename: D W
authors.initials: DW
authors.lastname: Brown
authors.forename: H L
authors.initials: HL
authors.lastname: Robinson
mesh_terms: D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000254:growth & development; D002478:Cells, Cultured; D002645:Chickens; D005814:Genes, Viral; D011991:Receptors, Virus / Q000502:physiology; D014759:Viral Envelope Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D011991:Receptors, Virus; D014759:Viral Envelope Proteins