Expression of hepatitis A virus cDNA in Escherichia coli: antigenic VP1 recombinant protein.
The genome of hepatitis A virus (HAV) was reverse transcribed into cDNA and molecularly cloned. cDNA clones coding for the capsid protein VP1 that carries the major HAV antigen were cloned into the expression vector pUR290 and expressed in Escherichia coli. The recombinant fusion protein reacted in an immunoblot with rabbit anti-HAV serum, suggesting that it possesses HAV antigenicity.
Ostermayr
R
R
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Federal Republic of Germany.
von der Helm
K
K
Gauss-Müller
V
V
Winnacker
E L
EL
Deinhardt
F
F
D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics*; D002213:Capsid / Q000032:analysis / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D035921:Hepatitis A Antigens; D006507:Hepatovirus / Q000235:genetics*; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis*
D000956:Antigens, Viral; D035921:Hepatitis A Antigens; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D004247:DNA; D004262:DNA Restriction Enzymes