Stimulation of 5-lipoxygenase activity in polymorphonuclear leukocytes of rats by caseinate treatment.
In the present study, the lipoxygenase activity in polymorphonuclear (PMN) leukocytes of rats was characterized, and the change in lipoxygenase activity in peritoneal and peripheral PMN leukocytes challenged by caseinate was investigated. Peritoneal PMN leukocytes produced 5-HETE and LTB4 as the major 5-lipoxygenase metabolites from arachidonic acid. The 5-lipoxygenase activity was calcium dependent. Caseinate treatment in rats significantly stimulated the 5-lipoxygenase activity in peripheral and peritoneal PMN leukocytes. Although it also stimulated the cyclooxygenase metabolic pathway, the effect was not as evident as that on the 5-lipoxygenase pathway. Since LTB4 is a potent chemotactic factor for PMN leukocyte migration, the present results might explain the mechanism on the PMN leukocyte infiltration caused by caseinate treatment.
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D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D001091:Arachidonate Lipoxygenases / Q000378:metabolism*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002364:Caseins / Q000494:pharmacology*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007975:Leukotriene B4 / Q000096:biosynthesis; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D010529:Peritoneal Cavity / Q000166:cytology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D001095:Arachidonic Acids; D002364:Caseins; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D016718:Arachidonic Acid; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D001091:Arachidonate Lipoxygenases; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D002118:Calcium