title: Introduction of new genetic material into human myeloid leukemic blast stem cells by retroviral infection.
abstract: An amphotropic retroviral vector containing the bacterial neomycin phosphotransferase gene (neo) was used to infect blast cells from patients with acute myeloblastic leukemia. The infected cells acquired a G418-resistant phenotype that was stable as measured in a clonogenic assay and in long-term suspension culture. Thus, gene transfer into stem cells was accomplished by this procedure. This approach for manipulating gene expression in blast stem cells provides a means to assess the roles of a variety of genes in self-renewal, differentiation, and leukemogenesis.
authors.affiliation: Department of Hematology, M.D. Anderson Hospital and Tumor Institute, Houston, Texas 77030.
authors.forename: L J
authors.initials: LJ
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Benchimol
mesh_terms: D001752:Blast Crisis / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D006801:Humans; D019868:Kanamycin Kinase; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000201:enzymology
chemical_list: D004273:DNA, Neoplasm; D010770:Phosphotransferases; D019868:Kanamycin Kinase