One-step extraction of human erythrocyte lipids allowing rapid determination of fatty acid composition.
A one-step extraction procedure to directly quantify cholesterol, phospholipids, and fatty acids in red blood cell membranes has been developed. The method uses a single solvent, isopropanol, which extracts lipids and allows the rapid formation of isopropylic esters of fatty acids by acid catalysis. The efficiency of this new technique has been confirmed by comparing yields of cholesterol and total and individual phospholipids with yields obtained following conventional extraction procedures. Moreover, in comparison to the formation of methyl esters, we demonstrate that directly obtained isopropylic esters immediately allow the quantitative determination of fatty acids, without involving the hydrolytic degradation of fatty acids and the oxidation of unsaturated fatty acids.
Peuchant
E
E
Department of Biochemistry, Saint-André Hospital, Bordeaux, France.
Wolff
R
R
Salles
C
C
Jensen
R
R
D002725:Chloroform; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004910:Erythrocyte Membrane / Q000032:analysis*; D005227:Fatty Acids / Q000097:blood*; D006801:Humans; D008563:Membrane Lipids / Q000097:blood*; D000432:Methanol; D012997:Solvents
D005227:Fatty Acids; D008563:Membrane Lipids; D012997:Solvents; D002725:Chloroform; D000432:Methanol