Sequence and centromere proximal location of a transformation enhancing fragment ans1 from Aspergillus nidulans.
The Aspergillus nidulans sequence ans1, previously known to enhance transformation frequencies of pyr4-based vectors, was shown to enhance the efficiency of argB and trpC-based vectors. Increased efficiencies could be obtained by constructing vectors containing argB and ans1 or by cotransforming selectable plasmids (containing argB, trpC, or pyr4) with the non-selectable ans1 sequence. The preponderance of evidence suggests that the mechanism of ans1 activity does not involve homologous recombination events, in spite of the presence of multiple regions of homology in the A. nidulans genome. Genetic mapping localized ans1 to the vicinity of the centromere of linkage group I. The nucleotide sequence of a 1.8 Kb functional subclone of ans1 was determined and found to be highly A + T rich (81%).
Cullen
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D
Genencor Inc., South San Francisco, CA 94080.
Wilson
L J
LJ
Grey
G L
GL
Henner
D J
DJ
Turner
G
G
Ballance
D J
DJ
D001233:Aspergillus nidulans / Q000235:genetics*; D001483:Base Sequence; D002503:Centromere / Q000032:analysis*; D002875:Chromosomes / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004742:Enhancer Elements, Genetic; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D014170:Transformation, Genetic
D004262:DNA Restriction Enzymes