Curing of endogenous 2 micron DNA in yeast by recombinant vectors.
Passaging on selective media of yeast strains transformed with complete 2 micron vectors carrying TRP1, LEU2 or URA3 selective markers leads to curing of the resident endogenous 2 micron DNA in a majority of the population. Vector plasmids defective in FLP function are fixed as populations of A, A + B or B forms after 2 micron loss. Transformation with these plasmids offers a general method of obtaining cir degree derivatives of any yeast strain.
Harford
M N
MN
Molecular Genetics Department, Smith Kline-RIT, Rixensart, Belgium.
Peeters
M
M
D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes