Blockade of N-methyl-D-aspartate response in enzyme-treated rat hippocampal neurons.
The responses to excitatory and inhibitory amino acids have been investigated in isolated pyramidal cells from young and adult rat hippocampus using internal perfusion and 'concentration-clamp' techniques. The neurons dissociated in a purely mechanical way were sensitive to all excitatory amino acids (glutamate, kainate, quisqualate and N-methyl-D-aspartate (NMDA] and inhibitory amino acids (glycine, taurine and gamma-aminobutyric acid). The NMDA response was dramatically potentiated by adding glycine at threshold concentration (10(-6) M). The enzyme treatment of hippocampal slices selectively removed the NMDA sensitivity but did not alter all other pharmacological properties of voltage- and agonist-gated ion channels.
Akaike
N
N
Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Kaneda
M
M
Hori
N
N
Krishtal
O A
OA
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002469:Cell Separation / Q000379:methods*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006867:Hydrolases / Q000494:pharmacology*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D016202:N-Methylaspartate; D010206:Papain / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology
D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D001224:Aspartic Acid; D016202:N-Methylaspartate; D006867:Hydrolases; D014357:Trypsin; D010206:Papain