Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody. Brief report.
Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immunofluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3.
Lecomte
J
J
Institut Armand-Frappier, Centre de recherche en virologie, Québec, Canada.
Cainelli-Gebara
V
V
Mercier
G
G
Mansour
S
S
Talbot
P J
PJ
Lussier
G
G
Oth
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology; D003588:Cytopathogenic Effect, Viral; D006524:Hepatitis, Viral, Animal / Q000473:pathology / Q000517:prevention & control*; D008099:Liver / Q000473:pathology; D051379:Mice; D008805:Mice, Inbred A; D006517:Murine hepatitis virus / Q000276:immunology*; D009500:Neutralization Tests; D009698:Nucleoproteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D009698:Nucleoproteins; D014764:Viral Proteins