Comparative study with monospecific and monoclonal antibodies against a 65 K human cytomegalovirus protein.
Immunofluorescence assay using monospecific and monoclonal antibodies to the 65 K major protein of human cytomegalovirus (HCMV) was carried out to monitor the expression of this protein in infected cells. Regardless of differences in the reactivity of the monoclonal antibodies, as determined by immunoblotting and immunofluorescent staining, all stained cytoplasmic inclusion bodies localized to the site of the HCMV-induced receptor for the Fc portion of IgG, suggesting that most of the 65 K major protein of HCMV colocalizes with the HCMV-induced FcR.
Shimokawa
K
K
Department of Microbiology, Kanazawa Medical University, Ishikawa, Japan.
Bin
X
X
Furukawa
T
T
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D003587:Cytomegalovirus / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007158:Immunologic Techniques; D008970:Molecular Weight; D014764:Viral Proteins / Q000032:analysis*; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins