Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro.
The possibility of DNA-binding proteins interacting in vitro with the polymerase II transcriptional machinery was explored by using a competition assay with individual target sequences for enhancer-binding factors. Transcription factors binding to at least five specific enhancer sequences mediate the activity of the human cytomegalovirus immediate-early 1 gene in vitro. Furthermore, our data suggest that individual DNA-bound enhancer factors can interact with the promoter transcription complex.
Ghazal
P
P
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Lubon
H
H
Hennighausen
L
L
D003587:Cytomegalovirus / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005814:Genes, Viral; D007700:Kinetics; D010957:Plasmids; D012319:RNA Polymerase II / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D014157:Transcription Factors; D012319:RNA Polymerase II