Chemical synthesis, cloning and expression in mammalian cells of a gene coding for human tissue-type plasminogen activator.
A 1610-bp DNA duplex coding for human tissue-type plasminogen activator has been chemically synthesized using the phosphoramidite procedure, adapted for a custom-built gene synthesizer. The synthesizer, which was designed for both simplicity and speed, permits the rapid construction of relatively large genes and compares favorably in speed with alternative cDNA isolation procedures. The plasminogen activator gene has been expressed in mammalian cells and shown to produce authentic protein by an immuno-activity assay.
Bell
L D
LD
Searle Research and Development, Bucks, U.K.
Smith
J C
JC
Derbyshire
R
R
Finlay
M
M
Johnson
I
I
Gilbert
R
R
Slocombe
P
P
Cook
E
E
Richards
H
H
Clissold
P
P
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics; D004262:DNA Restriction Enzymes; D005796:Genes; D005813:Genes, Synthetic; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D010959:Tissue Plasminogen Activator / Q000235:genetics*; D014158:Transcription, Genetic
D004247:DNA; D004262:DNA Restriction Enzymes; D010959:Tissue Plasminogen Activator