Retrovirus-expressed hemagglutinin protects against lethal influenza virus infections.
An influenza virus hemagglutinin gene, H7, has been expressed in a replication-competent Schmidt-Ruppin Rous sarcoma virus-derived vector. This virus, P1/H7, expressed a glycosylated precursor of the H7 protein which was processed to a mature form and transported to the cell surface. The expressed H7 glycoprotein could not be detected in P1/H7 virus particles. A P1/H7 stock which expressed 5 to 10% of the level of H7 observed in influenza virus-infected chicken embryo fibroblasts was used to immunize 1-month-old chickens. This immunization resulted in low or undetectable levels of hemagglutination-inhibiting and neutralizing antibody. Despite the low serum response, challenge with a highly pathogenic H7N7 virus revealed complete protection against lethal infection.
Hunt
L A
LA
Department of Microbiology and Immunology, University of Louisville School of Medicine, Kentucky 40292.
Brown
D W
DW
Robinson
H L
HL
Naeve
C W
CW
Webster
R G
RG
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D001358:Avian Sarcoma Viruses / Q000235:genetics; D002642:Chick Embryo; D002645:Chickens; D005822:Genetic Vectors; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics*; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D011201:Poultry Diseases / Q000517:prevention & control*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*
D000914:Antibodies, Viral; D000941:Antigens; D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic