Investigation of the role of P130gag-fps in transformation: generation and use of a temperature-sensitive mutant P130gag-fps.
Changing Glu-1025 to Asp in Fujinami sarcoma virus P130gag-fps made the protein temperature sensitive for transformation and protein-tyrosine kinase activity. Another mutant, Phe-1073 P130gag-fps, lacking the major autophosphorylation site, has an extended latent period for transformation (G. A. Weinmaster, M. J. Zoller, M. Smith, E. Hinze, and T. Pawson, Cell 37:559-568, 1984). By introducing the Asp-1025 lesion into Phe-1073 P130gag-fps, we showed that this mutant protein is required for the maintenance of the transformed phenotype of Phe-1073 P130gag-fps-expressing cells.
Weinmaster
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GA
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138.
Hunter
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T
D000818:Animals; D001345:Autoradiography; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D015683:Gene Products, gag; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D010766:Phosphorylation; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D051381:Rats; D012191:Retroviridae Proteins / Q000235:genetics* / Q000502:physiology; D013696:Temperature; D014162:Transfection
D015683:Gene Products, gag; D009856:Oncogene Proteins, Viral; D012191:Retroviridae Proteins; D011505:Protein-Tyrosine Kinases