Immunoassay procedures to detect exposure to aflatoxin B1 and benzo(a)pyrene in animals and man at the DNA level.
Immunological methods were used to examine human liver for the presence of aflatoxin-DNA adducts and human lung for benzo(a)pyrene diol-epoxide DNA (BPDE-DNA) adducts. Eight liver samples obtained from Czechoslovakian patients with primary hepatocellular carcinoma were studied, seven of which had detectable anti-aflatoxin inhibitory material. Values ranged between 0.63 and 3.51 picomoles aflatoxin per mg DNA. In a separate, independent study performed in another laboratory the one sample with no aflatoxin bound to DNA also had no free aflatoxin present in the liver. In the case of the human lung DNA samples, 12 samples were examined, the samples having been removed during thoracic surgery, and five had detectable anti-BPDE-DNA antibody activity. The positive samples were all from smokers and had inhibitory values ranging from 4 to 12 femtomoles per mg DNA. Samples were prepared by immunoconcentration prior to analysis. These preliminary results support the view that immunological methods can be used to examine human tissue DNA for carcinogen adducts.
Garner
R C
RC
Cancer Reseach Unit, University of York, Heslington, UK.
Dvorackova
I
I
Tursi
F
F
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008099:Liver / Q000032:analysis; D008113:Liver Neoplasms / Q000032:analysis; D008168:Lung / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000348:Aflatoxins; D002273:Carcinogens; D001564:Benzo(a)pyrene; D004247:DNA; D016604:Aflatoxin B1