A rapid method for the isolation of functional human T lymphocytes using hydroxyapatite column fractionation.
Passage of peripheral blood lymphocytes through a column of hydroxyapatite resulted in a 7-20-fold depletion of immunoglobulin-bearing cells, a 20-fold depletion of monocytes, and a 1.3-fold enrichment of T cells. The effluent population was virtually devoid of B lymphocyte precursors and monocytes, whereas helper cell and suppressor cell populations remained intact. This method will facilitate the rapid preparation of T-enriched cell populations.
Tsuru
S
S
Department of Bacteriology, National Defense Medical College, Saitama, Japan.
Taniguchi
M
M
Tsugita
M
M
Sekiguchi
S
S
Nomoto
K
K
D001402:B-Lymphocytes / Q000166:cytology; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D017886:Durapatite; D005374:Filtration; D006801:Humans; D006882:Hydroxyapatites; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D006882:Hydroxyapatites; D017886:Durapatite