[Characteristics of action of precursors of carcinogenic nitroso-compounds on cell cultures].
On the primary rat lung cell cultures, a study was made of the transforming action of sodium nitrite (NN) and amidopyrine combination with the control for formation of carcinogen--N-nitrosodimethylamine (NDMA) in growth medium. The manifestation of transformation was registered by appearance of morphological changes and multilayer growth foci. As criteria for evaluation after 48 hour treatments with NDMA, NN and AP, were used DNA-synthetizing activity of cells, mitotic index, frequency of mitoses pathology, monolayer density. The effects of transforming dose of NN alone and in combination with AP were the same. But malignization (tumor development in newborn rats in the points of cell suspension inoculation) took place only after administration of NN in combination with AP, when carcinogen was formed. Theophylline decreased the action of NN-AP combination.
Osin'kovskaia
N D
ND
Rubenchik
B L
BL
Tikmin
L A
LA
Glavin
A A
AA
D000632:Aminopyrine / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000473:pathology; D002478:Cells, Cultured; D008168:Lung / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D009573:Nitrites / Q000378:metabolism*; D009603:Nitroso Compounds / Q000633:toxicity*; D051381:Rats; D012977:Sodium Nitrite / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology
D002273:Carcinogens; D009573:Nitrites; D009603:Nitroso Compounds; D000632:Aminopyrine; D013806:Theophylline; D012977:Sodium Nitrite; D001205:Ascorbic Acid
Osobennosti deĭstviia predshestvennikov kantserogennykh nitrozosoedineniĭ na kletochnye kul'tury.