Molecular cloning of three structurally related genes for chicken avidin.
The chicken genomic library was screened using the 32P-labelled 3'-end of avidin cDNA as a hybridization probe. A positive clone, lambda gAV12201, containing a 15-16 kb insert, was detected. The EcoRI subclones, pgAV0.4, pgAV1.8, pgAV3.3 and pgAV3.7 from the genomic clone were subjected to hybridization and restriction enzyme mapping analysis. The preliminary results suggest the existence of three structurally related genes for chicken avidin. Whether the natural gene is within the subclones can only be established when sequencing analyses of the subclones have been completed.
Keinänen
R A
RA
Department of Biomedical Sciences, University of Tampere, Finland.
Laukkanen
M L
ML
Kulomaa
M S
MS
D000818:Animals; D001360:Avidin / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005796:Genes; D009710:Nucleotide Mapping; D012689:Sequence Homology, Nucleic Acid
D001360:Avidin; D004247:DNA; D004262:DNA Restriction Enzymes