A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein.
A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HCl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158 degrees C for reaction times of 22.5 and 45 min with 7 M HCl and 10% TFA containing 0.1% phenol.
Tsugita
A
A
Life Science Institute, Science University of Tokyo, Chiba.
Uchida
T
T
Mewes
H W
HW
Ataka
T
T
D000596:Amino Acids / Q000032:analysis; D002213:Capsid / Q000378:metabolism; D003574:Cytochrome c Group / Q000032:analysis / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism; D005463:Fluoroacetates; D005934:Glucagon / Q000032:analysis / Q000378:metabolism; D006358:Hot Temperature; D006851:Hydrochloric Acid; D006868:Hydrolysis; D007700:Kinetics; D009029:Mosaic Viruses; D009211:Myoglobin / Q000032:analysis / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D014269:Trifluoroacetic Acid
D000596:Amino Acids; D003574:Cytochrome c Group; D004151:Dipeptides; D005463:Fluoroacetates; D009211:Myoglobin; D010455:Peptides; D011506:Proteins; D005934:Glucagon; D014269:Trifluoroacetic Acid; D006851:Hydrochloric Acid