Identification of a common ecotropic viral integration site, Evi-1, in the DNA of AKXD murine myeloid tumors.
AKXD-23 recombinant inbred mice develop myeloid tumors at a high frequency, unlike other AKXD recombinant inbred strains which develop B-cell lymphomas, T-cell lymphomas, or both. AKXD-23 myeloid tumors are monoclonal, and their DNA contains somatically acquired proviruses, suggesting that they are retrovirally induced. We identified a common site of ecotropic proviral integration that is present in the DNA of all AKXD-23 myeloid tumors that were analyzed and in the DNA of all myeloid tumors that occur in AKXD strains other than AKXD-23. We designated this locus Evi-1 (ecotropic viral integration site 1). Rearrangements in the Evi-1 locus were also detected in the DNA of a number of myeloid tumors and myeloid cell lines isolated from strains other than AKXD. In contrast, few Evi-1 rearrangements were detected in the DNA of T- or B-cell tumors. Evi-1 may thus identify a new proto-oncogene locus that is involved in myeloid disease.
Mucenski
M L
ML
Mammalian Genetics Laboratory, National Cancer Institute, Frederick, Maryland 21701.
Taylor
B A
BA
Ihle
J N
JN
Hartley
J W
JW
Morse
H C
HC
Jenkins
N A
NA
Copeland
N G
NG
D000818:Animals; D002472:Cell Transformation, Viral; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D007942:Leukemia, Experimental / Q000235:genetics*; D008223:Lymphoma / Q000235:genetics; D051379:Mice / Q000235:genetics*; D011519:Proto-Oncogenes; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics*
D004273:DNA, Neoplasm; D004279:DNA, Viral; D004262:DNA Restriction Enzymes