Isolation of a human sequence which complements a mammalian G1-specific temperature-sensitive mutant of the cell cycle.
We have isolated a human genomic sequence which corrects the temperature-sensitive phenotype of the G1-specific cell cycle mutant ts13. Transfection of a thymidine kinase negative (TK-) derivative of ts13 with high molecular weight human DNA from placenta of HeLa cells resulted in the isolation of thermocompetent transformants. These transformants were found to possess discrete Hind III fragments containing human Alu family sequences which were conserved in several independent secondary transformants. A transforming sequence was recovered from a HindIII recombinant bacteriophage library prepared from the DNA of one such secondary transformant. The transforming sequence was isolated as a 12.5 kb Hind III fragment.
Sauvé
G J
GJ
Department of Pathology, Temple University Medical School, Philadelphia, Pennsylvania 19140.
Shen
Y M
YM
Zannis-Hadjopoulos
M
M
Chang
C D
CD
Baserga
R
R
Hand
R
R
D002453:Cell Cycle; D002460:Cell Line; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005260:Female; D005796:Genes; D005816:Genetic Complementation Test; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007399:Interphase; D009154:Mutation; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D013696:Temperature; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection
D004251:DNA Transposable Elements; D013937:Thymidine Kinase