Multiple endogenous xenotropic and mink cell focus-forming murine leukemia virus-related transcripts are induced by polyclonal immune activators.
Northern (RNA) analyses were used to study the kinetics of induction of endogenous mink cell focus-forming (MCF) and xenotropic murine leukemia virus (MuLV)-related sequences in NFS and C57BL/6 mice injected with the polyclonal immune activators lipopolysaccharide (LPS), concanavalin A, and 8-bromoguanosine. All three mitogens induced 8.4-, 7.2-, 3.0-, and 1.8-kilobase (kb) MCF-related transcripts coordinately in the spleens of injected mice. Xenotropic MuLV-related expression was also rapidly induced in spleens by the three polyclonal immune activators, but in a noncoordinate manner: a distinct set of transcripts with different kinetics of expression was induced by each mitogen. MCF-related induction after LPS injection was both rapid and sustained; it began within 30 min and persisted for at least 8 days in the spleens of both NFS and C57BL/6 mice. LPS also caused prolonged induction of xenotropic transcripts in spleens of C57BL/6 but not NFS mice. The gld mutation, which causes polyclonal immune activation, induced 8.4-, 10.0-, and 13-kb MCF-related transcripts in C3H/HeJ mice without altering expression of 7.2-, 5.6-, 4.0-, 3.0-, or 1.8-kb MCF-related transcripts. The data demonstrate that individual endogenous MuLV-related transcripts can be induced coordinately or independently and suggest that expression of these transcripts is linked to early stages of lymphocyte activation.
Krieg
A M
AM
Cellular Immunology Section, National Institute of Arthritis and Musculoskeletal and Skin Disease, Bethesda, Maryland 20892.
Khan
A S
AS
Steinberg
A D
AD
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D005786:Gene Expression Regulation; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D007700:Kinetics; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008908:Mink Cell Focus-Inducing Viruses / Q000235:genetics* / Q000276:immunology; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics*; D013154:Spleen / Q000382:microbiology; D013950:Thymus Gland / Q000382:microbiology; D014158:Transcription, Genetic
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D012367:RNA, Viral; D003208:Concanavalin A; D006151:Guanosine; C035169:8-bromoguanosine