Functional characterization of a pyrimidine-rich element in the 5'-noncoding region of the yeast iso-1-cytochrome c gene.
A predominantly pyrimidine-rich sequence (purine in the template strand, 32 of 37 bases) is located between a functional TATA element and the corresponding transcription start site region of the Saccharomyces cerevisiae iso-1-cytochrome c (CYC1) gene. By using linker deletions and gene fusion techniques, the functional characteristics of this pyrimidine sequence were examined. Results indicate that the function of this element is to limit the accumulation of full-length mRNAs with 5' ends which map upstream of the pyrimidine-rich sequence. Data suggest that the 5'-noncoding region of the CYC1 gene possesses signals for mRNA 3'-end processing.
McNeil
J B
JB
Department of Microbiology, University of Toronto, Ontario, Canada.
D001483:Base Sequence; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000235:genetics*; D045304:Cytochromes c; D004720:Endonucleases; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011743:Pyrimidines / Q000032:analysis*; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D011743:Pyrimidines; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases