Cyclic cytidine monophosphate stimulates DNA synthesis by bovine mammary tissue in vitro.
Mammary gland biopsies were taken from midpregnant heifers (n = 4), cut into pieces .5 mm thick and 3 - 5 mm2 and incubated for 48 hours in Eagle's Minimum Essential Medium containing 0, .1 or 1 micrograms/ml insulin and 0, 10(-8), 10(-7), 10(-6), 10(-5), or 10(-4) M dibutyryl cyclic 3', 5', cytidine monophosphate (dbcCMP). With 0 or .1 microgram/ml insulin, dbcCMP decreased incorporation of tritiated thymidine into DNA. Similar declines in DNA synthesis were observed with sodium butyrate, suggesting that the decline was due to the butyrate rather than to a cyclic CMP-specific effect. With 1 micrograms/ml insulin, dbcCMP increased DNA synthesis. Higher levels of dbcCMP reduced DNA synthesis relative to 10(-6)M dbcCMP, as did sodium butyrate. Thus cCMP is capable of stimulating mammary growth.
Sheffield
L G
LG
Dairy Science Department, University of Wisconsin, Madison 53706.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002417:Cattle; D003563:Cyclic CMP / Q000031:analogs & derivatives / Q000494:pharmacology* / Q000502:physiology; D003597:Cytosine Nucleotides / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D007328:Insulin / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy
D002087:Butyrates; D003597:Cytosine Nucleotides; D007328:Insulin; D020148:Butyric Acid; D003563:Cyclic CMP; C053441:dibutyryl cyclic-3',5'-cytidine monophosphate; D004247:DNA