Collagenase eliminates the electrical responses of smooth muscle to catecholamines.
The effects of collagenase on the membrane response to catecholamines were studied with intracellular microelectrodes in the guinea-pig taenia caeci and main pulmonary artery. In the taenia, the inhibitory actions of adrenaline mediated through alpha- and beta-adrenoceptors were both nearly abolished by two 5-min treatments with 0.005% collagenase. In the pulmonary artery, the depolarizing action of noradrenaline mediated through beta-adrenoceptors was markedly reduced by three 5-min treatments with 0.01% collagenase.
Tokuno
H
H
Department of Physiology, School of Medicine, Nagoya University, Japan.
Tomita
T
T
D000818:Animals; D002395:Catecholamines / Q000494:pharmacology*; D004558:Electric Stimulation; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D003012:Microbial Collagenase / Q000494:pharmacology*; D008839:Microelectrodes; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D011433:Propranolol / Q000494:pharmacology
D002395:Catecholamines; D011433:Propranolol; D003012:Microbial Collagenase; D007545:Isoproterenol; D004837:Epinephrine