Inhibition of phase separation by reagents that prevent X-irradiation cataract in vivo.
Galactose, and the phosphorothioates, WR-77913 and WR-2721, which inhibit cataract produced by X-irradiation, were evaluated for their effects on the phase separation temperature, Tc, of calf lens homogenate. The reagents were added to nuclear homogenate and the change in Tc per mol, dTc/dC, was measured. Galactose decreased Tc, -65 degrees C mol-1, WR-77913 decreased Tc, -28 degrees C mol-1 and WR-2721 decreased Tc, -76 degrees C mol-1. These are the first phase separation inhibitors that can be used in vivo to study the inhibition of lens opacification.
Clark
J I
JI
Department of Biological Structure, University of Washington, School of Medicine, Seattle 98195.
Osgood
T B
TB
Trask
S J
SJ
D004999:Amifostine / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D002386:Cataract / Q000209:etiology / Q000517:prevention & control*; D002417:Cattle; D005690:Galactose / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000187:drug effects / Q000528:radiation effects; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013696:Temperature
D009946:Organothiophosphorus Compounds; D011838:Radiation-Sensitizing Agents; C035284:WR 77913; D004999:Amifostine; D005690:Galactose