[Use of collagenase from the hepatopancreas of the Kamchatka crab for isolating and culturing endothelial cells of the large vessels in man].
The application of hepatopancreas collagenase from crab Paralithodes camtschatica for the isolation and cultivation of the endothelial cells was studied in human umbilical vein endothelium. The comparison of the enzyme from crab hepatopancreas with collagenase from Clostridium histoliticum has shown that the number of viable cells isolated from human umbilical vein by the crab enzyme was lower than in the case of microbial collagenase. However, this difference was not significant for subsequent cultivation of cells. Harvesting of the endothelial cells from the substrate during cultivation was more effective in the case when collagenase from crab hepatopancreas was used. It was shown that crab collagenase, in contrast with microbiological collagenase, was not a metal-dependent enzyme.
Goncharov
N V
NV
Sakharov
I Iu
IIu
Danilov
S M
SM
Sakandelidze
O G
OG
D000818:Animals; D003386:Brachyura; D003584:Cytological Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008099:Liver / Q000201:enzymology; D003012:Microbial Collagenase; D010179:Pancreas / Q000201:enzymology; D014471:Umbilical Veins / Q000166:cytology
D003012:Microbial Collagenase
Ispol'zovanie kollegenazy iz gepatopankreasa kamchatskogo kraba dlia vydelenia i kul'tivirovaniia éndothelial'nykh kletok krupnykh sosudov cheloveka.