Highly glycosylated PDGF-like molecule secreted by simian sarcoma virus-transformed cells.
Antiserum to human platelet-derived growth factor (PDGF) recognized a simian sarcoma virus transformation-specific glycopeptide, now termed gp200sis, thereby establishing an immunological relationship between PDGF and this highly glycosylated molecule. The same antibodies as well as an antiserum against SSV-NP cells reacted with isolated gp200sis after immunoprecipitation, SDS-PAGE, and electroelution. In analogy to PDGF, the gp200sis protein backbone is shown here to consist of disulfide-linked polypeptide chains. On SDS-PAGE under nonreducing conditions, the deglycosylated molecule migrated as two dimers with molecular weights of 26 and 28 kDa, respectively. Preliminary functional studies indicate that SSV nonproducer cells secrete high-molecular-weight mitogens (greater than 150 kDa) that are specific for SSV-induced transformation. We suggest that gp200sis acts as a PDGF-like growth factor.
Klein
R
R
Federal Research Center for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Thiel
H J
HJ
D002472:Cell Transformation, Viral; D004220:Disulfides; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D046911:Macromolecular Substances; D008934:Mitogens; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000276:immunology / Q000378:metabolism*; D009857:Oncogenes; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000378:metabolism*; D011509:Proteoglycans / Q000276:immunology / Q000378:metabolism; D012190:Retroviridae / Q000378:metabolism*; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics / Q000378:metabolism*
D004220:Disulfides; D006023:Glycoproteins; D046911:Macromolecular Substances; D008934:Mitogens; D009856:Oncogene Proteins, Viral; D010982:Platelet-Derived Growth Factor; D011509:Proteoglycans