Serologic and pathogenetic studies on avian reoviruses isolated in Japan.
Eighty-nine avian reoviruses isolated from diseased and clinically normal chickens were classified serologically using antisera against five prototype strains. Eighty-three strains were classified into five serotypes; six strains were untypable. Most of the cytopathogenic strains that produced a clear cytopathic effect (CPE) in chicken embryo fibroblasts (CEFs) were highly pathogenic for chicken embryos (80% or more mortality via the allantoic sac) and for chicks (severe footpad swellings and tenosynovitis). These strains were classified into a single serotype represented by the TS-142 prototype strain. However, 10 strains that could not produce a clear CPE in CEFs showed very low pathogenicity for embryos and chicks, and these strains were serologically different from the TS-142 prototype strain. There was a strong relationship between pathogenicity and serotype. About 17% of the isolates were considered highly pathogenic.
Takase
K
K
Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan.
Nonaka
F
F
Yamamoto
M
M
Yamada
S
S
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts; D007106:Immune Sera / Q000276:immunology; D007564:Japan; D011201:Poultry Diseases / Q000382:microbiology*; D012087:Reoviridae / Q000145:classification / Q000276:immunology / Q000472:pathogenicity*; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D012703:Serotyping / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral; D007106:Immune Sera