Restriction patterns of model DNA treated with 5,6-dihydroxyindole, a potent cytotoxic intermediate of melanin synthesis: effect of u.v. irradiation.
The interaction of 5,6-dihydroxyindole, a putative cytotoxic intermediate of melanin synthesis, with model lambda phage DNA has been investigated by using type II restriction endonucleases and CsCl buoyant density centrifugation. As evidenced by agarose gel electrophoresis and density gradient profiles, the 5,6-dihydroxyindole or u.v. treated DNAs, restricted or not, are modified. U.v. irradiation enhances 5,6-dihydroxyindole binding to DNA, but no sequence specific binding was observed. The action of L-3,4-dihydroxyphenylalanine on the restriction patterns of lambda phage DNA was also investigated and the effect appeared smaller, by qualitative evaluation, than that produced by 5,6-dihydroxyindole.
Miranda
M
M
Department of Cell Biology and Physiology, University of L'Aquila-via Assergi, Italy.
Bonfigli
A
A
Zarivi
O
O
Manilla
A
A
Cimini
A M
AM
Arcadi
A
A
D010582:Bacteriophage lambda / Q000235:genetics; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000528:radiation effects; D004279:DNA, Viral / Q000187:drug effects / Q000528:radiation effects; D007211:Indoles / Q000494:pharmacology*; D007980:Levodopa / Q000494:pharmacology; D008543:Melanins / Q000096:biosynthesis; D014466:Ultraviolet Rays
D004279:DNA, Viral; D007211:Indoles; D008543:Melanins; D007980:Levodopa; D004247:DNA; D004262:DNA Restriction Enzymes; C033871:5,6-dihydroxyindole