Alkaline nuclease activity in cells infected with herpes simplex virus type 1 (HSV-1) and HSV-1 temperature-sensitive mutants.
An in situ assay has been adapted to the herpes simplex virus type 1 (HSV-1) system which can detect alkaline nuclease activity in infected cell lysates following sodium dodecylsulfate polyacrylamide gel electrophoresis. Lysates of cells infected with HSV-1 temperature-sensitive (ts) mutants possessing mutations in the genes for an immediate-early transcriptional regulatory protein (ICP4), viral DNA polymerase (pol), and the major HSV-1 DNA binding protein (ICP8) exhibited altered alkaline nuclease profiles relative to that of wild-type virus-infected cells at 39 degrees C. Infections with a control mutant defective in the gene for glycoprotein B yielded wild-type nuclease profiles. The diverse effects on alkaline nuclease expression of mutants with lesions in different viral proteins involved directly in viral DNA synthesis provides evidence for the cooperative interaction between HSV-encoded viral DNA replication components.
Hafner
J
J
Department of Microbiology, University of New Hampshire, Durham 03824.
Mohammad
F
F
Farber
F E
FE
D000818:Animals; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D003851:Deoxyribonucleases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D017874:Immediate-Early Proteins; D008970:Molecular Weight; D009154:Mutation; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D014764:Viral Proteins; C048925:herpes simplex virus, type 1 protein ICP4; D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases