[Cyclic nucleotide phosphodiesterase of the olfactory membrane in rats: its distribution and possible connection with the estradiol receptor].
In the rat olfactory tissue the existence of soluble and membrane-bound forms of cyclic nucleotide phosphodiesterase (PDE) with quite similar kinetic parameters was demonstrated (KM = 220 and 200 microM, VMaX = 10 and 7 nmoles cAMP/mg protein per minute, respectively). 17 beta-estradiol (10(-7)-10(-5) M) decreased the soluble PDE activity by 25%, whereas non-hydrolysable GTP analogue (Gpp (NHp)) abolished their effect. On the other hand, this analogue in a dose-dependent manner inhibited the specific binding of 3H-estradiol to cytosolic receptors. The data indicate possible functional interrelations between the cytosolic estradiol receptors, GTP-binding proteins and PDE in the olfactory tissue which is a target organ for steroid hormones.
Parfenova
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D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007700:Kinetics; D009831:Olfactory Mucosa / Q000187:drug effects / Q000201:enzymology*; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D011959:Receptors, Estradiol / Q000187:drug effects / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D012995:Solubility
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; D006165:Guanylyl Imidodiphosphate; D004958:Estradiol; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D019204:GTP-Binding Proteins
Fosfodiésteraza tsiklicheskikh nukleotidov oboniatel'noĭ vystilki krys: raspredelenie i vozmozhnaia sviaz' s retseptorom éstradiola.