A new densitometric procedure to measure protein levels in tissue slices used in quantitative autoradiography.
A new quantitative staining technique for the assay of protein in tissue sections using the dye Coomassie brilliant blue G 250 is discussed. This densitometric procedure uses commercially available hardware and software employed in the quantitation of receptor autoradiographs. Rather than assuming a homogeneous distribution of protein in the tissue section, regional levels of protein are measured in the same tissue slice used to produce the autoradiograph. Additionally, the process of staining tissue with Coomassie blue for protein is reversible; the tissue can be destained after the measurement of protein is complete and then restained with a standard histological stain such as Cresyl violet. This technique is a more reliable method for normalizing receptor densities by tissue protein levels and allows for a more accurate comparison between QAR and membrane binding techniques.
Miller
J A
JA
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
Curella
P
P
Zahniser
N R
NR
D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry; D003720:Densitometry / Q000379:methods; D007457:Iodine Radioisotopes; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009928:Organ Specificity; D010869:Pindolol; D011506:Proteins / Q000032:analysis*; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000032:analysis
D007457:Iodine Radioisotopes; D009419:Nerve Tissue Proteins; D011506:Proteins; D011943:Receptors, Adrenergic, beta; D010869:Pindolol