title: [Properties of cyclic nucleotide phosphodiesterase from plasma membranes of rat synaptosomes at early stages of acute radiation injury].
abstract: Phosphodiesterase of cyclic nucleotides from membranes of rat brain synaptosomes hydrolyzes cAMP and cGMP; the maximal rate of cAMP hydrolysis is 2.5 times higher than the values of the analogous index for GMP. The enzyme is found to be activated by calmodulin. A different direction of changes in the rate of cAMP and cGMP hydrolysis is observed 1 h after total X-ray irradiation. The process of cAMP hydrolysis by phosphodiesterase is characterized by positive cooperativity which is also shown after irradiation and for the process of cGMP hydrolysis. It is established that enzyme inhibition by the reaction product takes place both in control and after irradiation.
authors.forename: T I
authors.initials: TI
authors.lastname: Parkhomets
authors.forename: A N
authors.initials: AN
authors.lastname: Vasil'ev
authors.forename: N E
authors.initials: NE
authors.lastname: Kucherenko
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000528:radiation effects; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D011833:Radiation Injuries, Experimental / Q000201:enzymology*; D051381:Rats; D013574:Synaptosomes / Q000201:enzymology*; D013997:Time Factors
chemical_list: D000242:Cyclic AMP; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D006152:Cyclic GMP
vernacular_title: Svoĭstva fosfodiésterazy tsiklicheskikh nukleotidov plazmaticheskikhmembran sinaptosom golovnogo mozga krys na rannikh étapakh ostrogo luchevogo vozdeĭstviia.