A simple assay to estimate the acetylcholinesterase molecular forms in crude extracts of rat skeletal muscle.
All the current methods available for analyzing the acetylcholinesterase (AChE) molecular forms are time consuming and require the use of expensive equipment. We have found that by using the differential inactivation of globular (G4 + G1) and asymmetric AChE forms by high Mg2+ concentration, we can set up a very easy and quick assay that allows us to determine the relative proportions of AChE molecular forms present in rat skeletal muscles. This assay will be of great help in estimating changes in the muscle AChE forms under experimental conditions that require several simultaneous determinations.
Perelman
A
A
Department of Cell and Molecule Biology, Faculty of Biological Sciences, Catholic University of Chile, Santiago.
Inestrosa
N C
NC
D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D003964:Diaphragm; D007527:Isoenzymes / Q000032:analysis*; D015636:Magnesium Chloride / Q000494:pharmacology; D008297:Male; D009132:Muscles / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D015636:Magnesium Chloride; D000110:Acetylcholinesterase