Stimulated T cell and natural killer (NK) cell lines fail to synthesize leukotriene B4.
The ability of leukotriene B4 (LTB4) to influence T cell and natural killer (NK) cell functions makes the question of LTB4 generation by these cells important to address. Consequently, LTB4 generation was evaluated in a human (Jurkat), and in a murine (EL-4) T cell line as well as in a rat NK cell line (RNK-16). Incubation of each of the 3 cell lines with [1-14C]arachidonic acid alone or in the presence of phytohemagglutinin (PHA), of calcium ionophore A23187, or of concanavalin A (Con A) plus the phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA) failed to generate radiolabelled LTB4 or other eicosanoids as determined by thin layer radiochromatography. Using two different radioimmunoassays for LTB4 also failed to demonstrate the generation of LTB4 under basal or stimulated conditions. These results support earlier studies that demonstrate that T cells are not capable of de novo synthesis of prostaglandins, thromboxanes, or leukotrienes and also provide evidence that NK cells also do not have the capacity to generate LTB4 or other eicosanoids. Our findings are also critically discussed in relation to studies claiming eicosanoid synthesis by T cells.
Goldyne
M E
ME
Department of Dermatology, Veterans Administration Medical Center, San Francisco, California 94121.
Rea
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L
D001095:Arachidonic Acids / Q000378:metabolism; D002460:Cell Line; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000378:metabolism*
D001095:Arachidonic Acids; D007376:Interleukin-2; D007975:Leukotriene B4