On the specificity of pig adrenal ferredoxin (adrenodoxin) and spinach ferredoxin in electron-transfer reactions.
Spinach leaf ferredoxin and ferredoxin:NADP oxidoreductase as well as pig adrenodoxin and adrenodoxin reductase have been purified to homogeneity. Ferredoxin-NADP reductase and adrenodoxin-NADP reductase can perform the same diaphorase reactions (dichloroindophenol, ferricyanide and cytochrome c reduction) albeit not with the same efficiency. Despite the differences in their redox potentials, animal and plant ferredoxins can be used as heterologous substrates by the ferredoxin-NADP reductases from both sources. In heterologous systems, however, the ferredoxin/adrenodoxin concentrations must be increased approximately 100-fold in order to reach rates similar to those obtained in homologous systems. Ferredoxin and adrenodoxin can form complexes with the heterologous reductases as demonstrated by binding experiments on ferredoxin-Sepharose or ferredoxin-NADP-reductase-Sepharose and by the realization of difference spectra. Adrenodoxin also weakly substitutes for ferredoxin in NADP photoreduction, and can be used as an electron carrier in the light activation of the chloroplastic enzyme NADP-dependent malate dehydrogenase. In addition adrenodoxin is a good catalyst of pseudocyclic photophosphorylation, but not of cyclic phosphorylation and can serve as a substrate of glutamate synthase. These results are discussed with respect to the known structures of plant and animals ferredoxins and their respective reductases.
Jacquot
J P
JP
Laboratoire de Physiologie végétale moléculaire, Université de Paris Sud, Orsay, France.
Suzuki
A
A
Peyre
J B
JB
Peyronnet
R
R
Miginiac-Maslow
M
M
Gadal
P
P
D000311:Adrenal Glands / Q000201:enzymology*; D000325:Adrenodoxin / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism; D004579:Electron Transport; D005287:Ferredoxin-NADP Reductase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism*; D005779:Immunodiffusion; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000378:metabolism; D013552:Swine
D003574:Cytochrome c Group; D005288:Ferredoxins; D010940:Plant Proteins; D000325:Adrenodoxin; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases