Enkephalin-binding protein in human blood.
An enkephalin-binding protein was found in human plasma and serum. The protein was partially purified by DEAE-cellulose column chromatography. The binding of [3H]leucine-enkephalin to this protein was competitively inhibited by unlabeled leucine- and methionine-enkephalin and various peptide hormones such as beta-endorphin and glucagon, but not by Leu-enkephalin-amide. The fact that amide derivatives of leucine-enkephalin and methionine-enkephalin did not inhibit the binding suggests that c-terminuses of enkephalins might have an important part in binding the protein. From these results, physiological roles of the enkephalin-binding protein are discussed.
Nakajima
S
S
Department of Anesthesiology, School of Medicine, Juntendo University, Tokyo, Japan.
Komuro
T
T
Shimamura
M
M
Hazato
T
T
D001667:Binding, Competitive; D001798:Blood Proteins / Q000302:isolation & purification*; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D004743:Enkephalin, Leucine / Q000097:blood; D004745:Enkephalins / Q000097:blood*; D006801:Humans; D011485:Protein Binding
D001798:Blood Proteins; D002352:Carrier Proteins; D004745:Enkephalins; D004743:Enkephalin, Leucine