Expression of the oxi1 and maturase-related RF1 genes in yeast mitochondria.
Transcription of the yeast mitochondrial oxi1 gene (cytochrome oxidase subunit 2) is initiated at a variant non anucleotide sequence, TTAAAAGTA, located 54 bp upstream from the protein-coding gene. Transcriptional initiation at this site gives rise to a 2,500 nucleotide primary transcript containing both the oxi1 gene and the downstream maturase-related reading frame, RF1. Precise transcript mapping has revealed that the 3'-end of the mature oxi1 mRNA is generated by an endonucleolytic cleavage which takes place after the conserved dodecamer sequence, AAUAAUAUUCUU (End-of-Messenger signal), 75 nucleotides downstream from the oxi1 stop codon. Since the RF1 5'-terminal coding region overlaps the oxi1 3'-terminal coding sequence, cleavage at this motif truncates the RF1 message suggesting that the expression of the putative RF1 protein is controlled at the level of dodecamer processing.
Bordonné
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Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
Dirheimer
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G
Martin
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RP
D003576:Electron Transport Complex IV / Q000235:genetics*; D004722:Endoribonucleases; D005796:Genes; D005800:Genes, Fungal; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D012333:RNA, Messenger; D003576:Electron Transport Complex IV; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases