[Characteristics of Klebsiella pneumoniae plasmid bearing the markers of drug resistance and antilysozyme activity].
Electrophoretic study of the profile of plasmid DNA in agarose gel has shown the presence of a plasmid with a molecular weight of 55-60 MD in K. pneumoniae strains possessing antilysozyme activity. Plasmid pAlz60 of K. pneumoniae 22-110, isolated from the blood of a septicemia patient, is a fi- type conjugative plasmid. This plasmid is transferred to recipient strains of different species of enterobacteria with a frequency of 1 X 10(-5) to 1 X 10(-7). Simultaneously with the transfer of the plasmid, recipient cells inherit the antilysozyme markers and resistance to a number of drugs. The discovered plasmid has one restriction site for each of endonucleases EcoRI and XhoI and 16-20 sites for restrictases KpNI, BglII and Hind III.
Bondarenko
V M
VM
Iablochkov
A L
AL
Romanova
Iu M
IuM
Petrovskaia
V G
VG
D003227:Conjugation, Genetic / Q000187:drug effects; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005819:Genetic Markers / Q000187:drug effects; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008970:Molecular Weight; D009113:Muramidase / Q000037:antagonists & inhibitors*; D010957:Plasmids / Q000187:drug effects
D004269:DNA, Bacterial; D005819:Genetic Markers; D004262:DNA Restriction Enzymes; D009113:Muramidase
Kharakteristika plazmidy Klebsiella pneumoniae, nesushcheĭ markery lekarstvennoĭ ustoĭchivosti i antilizotsimnoĭ aktivnosti.