title: Tissue distribution of calcium-activated neutral proteinases in rat.
abstract: A simple separation method involving a minimum number of essential steps was established to separate the two kinds of calcium-activated neutral proteinase (CANP) activity from each other and from endogenous CANP inhibitor activity. Determination of CANP activity in rat tissues using this method revealed a ubiquitous distribution of two CANPs. The level of CANP activity, however, differs between tissues. Low-calcium-requiring CANP (microCANP) is plentiful in spleen and kidney, while high-calcium-requiring CANP (mCANP) is plentiful in lung and brain. In all of the tissues examined, mCANP activity predominates over microCANP activity.
authors.affiliation: Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kawashima
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Kasai
authors.forename: K
authors.initials: K
authors.lastname: Imahori
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002154:Calpain / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D007668:Kidney / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D013154:Spleen / Q000201:enzymology; D014018:Tissue Distribution
chemical_list: D002154:Calpain; D002118:Calcium