[Establishment of a human colonic carcinoma xenograft in nude mice and its chief biological characteristics].
A human colonic cancer specimen cut into 1-2 mm3 pieces under aseptic condition was heterotransplanted hypodermically by trocar to the inguinal region of BALB/cATcL nude mice bred in our laboratory. 53 days later, a 10 X 9 X 11 mm3 tumor was obtained. The take rate was 100% (48/48). The take rate of liquid nitrogen frozen and recovered tissue pieces was also 100% (13/13). Grossly, the tumor was rich in blood supply and well encapsulated. Within the tumor, the tissue was cream-colored. G-banded chromosome analysis revealed a human chromosome pattern with the mode 70-90. Pathologic diagnosis was signet-ring cell cancer, which was identical to the surgical specimen. No change was found after maintaining 13 passages in nude mice. It was named the human colonic carcinoma xenograft XHCn/w. It provides an ideal tumor model for in vivo or in vitro experiments.
Wang
J
J
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Mi
F H
FH
Su
X Q
XQ
Li
Y C
YC
Deng
J S
JS
Li
S D
SD
Xu
D X
DX
Shen
Y
Y
Gu
X Z
XZ
D002288:Adenocarcinoma, Mucinous / Q000235:genetics / Q000473:pathology*; D000818:Animals; D002277:Carcinoma / Q000235:genetics / Q000473:pathology*; D002871:Chromosome Banding; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009368:Neoplasm Transplantation