title: Rabbit antibodies against the low molecular weight folate binding protein from human milk. Use for immunological characterization of human folate binding proteins in an enzyme-linked immunosorbent assay (ELISA).
abstract: Antibodies to a low molecular weight folate binding protein isolated from human milk were raised in rabbits and used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for immunological characterization of human folate binding proteins (FBPs). The high and low molecular weight FBPs from human milk were immunologically indistinguishable. Furthermore, the FBPs in human urine and cerebrospinal fluid showed a cross-reactivity of 70% and 30%, respectively. No cross-reactivity of the FBP from cow's milk was observed.
authors.affiliation: Laboratory for Autoimmune Serology, State Serum Institute, Copenhagen, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Høier-Madsen
authors.forename: S I
authors.initials: SI
authors.lastname: Hansen
authors.forename: J
authors.initials: J
authors.lastname: Holm
mesh_terms: D000906:Antibodies; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000032:analysis*; D006801:Humans; D008895:Milk, Human / Q000032:analysis*; D008970:Molecular Weight; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000032:analysis*; D014316:Tritium
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D014316:Tritium; D005492:Folic Acid