Distribution and colocalization of delta sleep inducing peptide (DSIP) with corticotropin-like intermediate lobe peptide (CLIP) in the human hypophysis.
DSIP and CLIP [ACTH(18-39)] immunoreactive (IR) neurons and fibers were examined in the human hypophysis and pituitary stalk using immunmohistofluorescence and peroxidase-antiperoxidase methods. Double-stained and adjacent stained sections demonstrate that DSIP is colocalized in about 75% of CLIP-IR-like cells in the anterior pituitary and in residual intermediate lobe cells. Only few CLIP-IR-like fibers are observed in the posterior lobe. On the contrary, a high density of DSIP-IR fibers is visualized in the stalk. It is suggested that DSIP acts as a sleep promoting factor (one of many other actions) and that CLIP increases the paradoxical sleep, so that these two peptides could play a role in the regulating system of the sleep-waking cycle.
Vallet
P G
PG
Division of Morphological Psychopathology, University, Department of Psychiatry, Geneva, Switzerland.
Charnay
Y
Y
Bouras
C
C
Constantinidis
J
J
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D053497:Corticotropin-Like Intermediate Lobe Peptide; D003701:Delta Sleep-Inducing Peptide / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D010446:Peptide Fragments / Q000032:analysis*; D010902:Pituitary Gland / Q000032:analysis*
D010446:Peptide Fragments; D053497:Corticotropin-Like Intermediate Lobe Peptide; D003701:Delta Sleep-Inducing Peptide; D000324:Adrenocorticotropic Hormone