Platelet surface glycosaminoglycans are an effective shield for distinct platelet receptors.
The presence of glycosaminoglycans on the platelet surface was demonstrated by electronmicroscopy and biochemical analysis. Chondroitin ABC lyase was able to remove a substantial portion of the Ruthenium red-stained outer coat of platelets. Analysis of the reaction product released by the enzyme revealed chondroitin 4-sulfate. To determine the biological function of this glycosaminoglycan coat, binding studies with a variety of potential platelet ligands were performed. In decreasing order of effectiveness, chondroitin ABC lyase was able to increase the binding sites of von Willebrand factor, fibrinogen, antibody to platelet-specific antigen P1A1, Fc fragments of IgG, and monomeric IgG. No change in binding was observed with F(ab)2 fragments of IgG, wheat germ agglutinin and pokeweed mitogen. These studies indicate that glycosaminoglycans shield some platelet receptor sites from their respective ligands. Upon release of the heteropolysaccharide from the platelet surface more of these sites become accessible to the ligand. It may be significant that especially glycoproteins involved in platelet adhesion and aggregation are involved in this process.
Steiner
M
M
Division of Hematology/Oncology, Memorial Hospital of Rhode Island, Pawtucket 02860.
D001792:Blood Platelets / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D002462:Cell Membrane / Q000032:analysis*; D002808:Chondroitin Lyases / Q000494:pharmacology; D002809:Chondroitin Sulfates / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D011956:Receptors, Cell Surface / Q000032:analysis*; D011955:Receptors, Drug / Q000032:analysis*
D006025:Glycosaminoglycans; D011956:Receptors, Cell Surface; D011955:Receptors, Drug; D002809:Chondroitin Sulfates; D002808:Chondroitin Lyases