Labeling of neural cells by gold-filled Sendai virus envelopes before intracerebral transplantation.
Gold-filled Sendai virus envelopes were fused with cell suspensions from the basal forebrain of fetal rat donors, and the resulting gold-labeled cells were transplanted into the neocortex of adult rat recipients. Not only did large numbers of labeled cells remain intact through 3 months in the neocortex, but sizable numbers migrated subcortically to the recipient's lesioned nucleus basalis region (a distance of 4 to 5 millimeters). Since this technique is capable of labeling most transplanted cells for long periods of time, it may be useful in determining the survival, migration, and connectivity of intracerebrally transplanted tissues.
Ardizzoni
S C
SC
Department of Biology, University of South Florida, Tampa 33620.
Michaels
A
A
Arendash
G W
GW
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology; D005333:Fetus; D006046:Gold; D009417:Nerve Tissue / Q000637:transplantation*; D009474:Neurons / Q000166:cytology / Q000637:transplantation*; D010222:Parainfluenza Virus 1, Human; D051381:Rats; D014759:Viral Envelope Proteins
D014759:Viral Envelope Proteins; D006046:Gold