Direct molecular analysis of a deletion of 3p in tumors from patients with sporadic renal cell carcinoma.
Normal and tumorous nephrectomy specimens from seven renal cell carcinoma patients were subjected to a Southern analysis using chromosome #3-specific polymorphic probes. Three patients were not informative because of homozygosity at all loci studied. One patient showing heterozygosity at 3q in normal tissue had a tumor that remained heterozygous. In three patients the tumor showed loss of heterozygosity for a short arm market at 3p21. In one of them heterozygosity for a second short arm marker was also lost. Another of these three patients retained heterozygosity for this second short arm marker, as well as for a long arm marker, suggesting a chromosomal breakpoint between the loci for the two short arm markers. Our results demonstrate that the known involvement of a short arm region of chromosome #3 in the development of renal cell carcinoma can readily be further evaluated by direct molecular methods.
van der Hout
A H
AH
Department of Human Genetics, State University of Groningen, The Netherlands.
Kok
K
K
van den Berg
A
A
Oosterhuis
J W
JW
Carritt
B
B
Buys
C H
CH
D000483:Alleles; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002872:Chromosome Deletion; D002893:Chromosomes, Human, Pair 3; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics; D015247:Deoxyribonuclease HindIII; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization
D004273:DNA, Neoplasm; D005819:Genetic Markers; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII