Chromosome replication in Escherichia coli induced by oversupply of DnaA.
Overexpression of DnaA protein from a multicopy plasmid accompanied by a shift to 42 degrees C causes initiation of one extra round of replication in a dnaA+ strain grown in glycerol minimal medium. This extra round of replication does not lead to an extra cell division, such that cells contain twice the normal number of chromosomes.
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Biology Programs, University of Texas at Dallas, Richardson 75080.
Bremer
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D001426:Bacterial Proteins / Q000502:physiology*; D002455:Cell Division; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D013696:Temperature
D001426:Bacterial Proteins; D004269:DNA, Bacterial