Transposition of IS50L activates downstream genes.
A transposition system constructed to detect the transposition of Tn5 to a site upstream of the lacZ gene has revealed that transposition of IS50L can activate downstream genes. Expression is apparently mediated by the NPTII promoter. Transposase produced either by IS50R or by the suppressed IS50L catalyzed transposition of IS50L.
Kendrick
K E
KE
Department of Biochemistry, University of Wisconsin, Madison 53706.
Reznikoff
W S
WS
D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes