title: Molecular cloning of complementary DNA for human medullasin: an inflammatory serine protease in bone marrow cells.
abstract: Medullasin, an inflammatory serine protease in bone marrow cells, modifies the functions of natural killer cells, monocytes, and granulocytes. We have cloned a medullasin cDNA from a human acute promyelocytic cell (ML3) cDNA library using oligonucleotide probes synthesized from the information of N-terminal amino acid sequence of natural medullasin. The cDNA contained a long open reading frame encoding 237 amino acid residues beginning from the second amino acid of natural meduallasin. The deduced amino acid sequence of medullasin shows a typical serine protease structure, with 41% homology with pig elastase 1.
authors.affiliation: Basic Research Laboratories, Toray Industries, Inc., Kanagawa.
authors.forename: K
authors.initials: K
authors.lastname: Okano
authors.forename: Y
authors.initials: Y
authors.lastname: Aoki
authors.forename: T
authors.initials: T
authors.lastname: Sakurai
authors.forename: M
authors.initials: M
authors.lastname: Kajitani
authors.forename: S
authors.initials: S
authors.lastname: Kanai
authors.forename: T
authors.initials: T
authors.lastname: Shimazu
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: M
authors.initials: M
authors.lastname: Naruto
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001853:Bone Marrow / Q000201:enzymology*; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D006801:Humans; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000235:genetics*
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes; D012697:Serine Endopeptidases; C034869:medullasin