Purification and characterization of an endonuclease (E.C. 3.1.30.1) from Streptomyces tendae.
A single-strand-specific endonuclease which converted negatively supercoiled DNA to open-circular and linear DNA was purified to homogeneity with Hb-Sepharose 4B, DEAE Trisacryl M, HA-Ultrogel and PBE-94 chromatofocusing from extracts of Streptomyces tendae ATCC 31160. Bio-Gel P-200 chromatography and electrophoresis in SDS-PAGE indicated the native protein was a monomer with a molecular weight of approximately 40-kDa. This enzyme did not hydrolyze double-stranded linear DNA but digested RNA and circular single-strand DNA. Sequence specificity for nicking of negatively supercoiled DNA was not detected.
Engel
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Southern Regional Research Laboratory, ARS, New Orleans, Louisiana 70124.
Ullah
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D005591:Chemical Fractionation; D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000032:analysis / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013302:Streptomyces / Q000201:enzymology*
D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases