[Detection of genomic RNA of the hepatitis A virus in clinical samples using the blot hybridization method].
The synthetic oligonucleotide sequence 3'CTCCTCGTACTTTA-5' complementing hepatitis A virus RNA was compared with cDNA probes in identification of viral genomic RNA. The clinical materials from patients in the 1-2 weeks of jaundice were screened. High specificity of the technique was demonstrated. Possibility of clinical using of the blot hybridization technique is discussed.
Nepliuev
I V
IV
Doroshenko
N V
NV
Stakhanova
V M
VM
Mesianzhinov
V V
VV
Zhdanov
V M
VM
D004247:DNA / Q000235:genetics; D005243:Feces / Q000382:microbiology; D006506:Hepatitis A / Q000175:diagnosis* / Q000382:microbiology; D006507:Hepatovirus / Q000032:analysis* / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics
D012367:RNA, Viral; D004247:DNA
Detektsiia genomnoĭ RNK virusa gepatita A v klinicheskikh obraztsakh s pomoshch'iu metoda tochechno gibridizatsii.