The obtention of simian virus 40 recombinants carrying d(CG.GC)n, d(CA.GT)n and d(CT.GA)n sequences. Stability of the inserted simple repeating sequences.
A general strategy for the introduction of simple repeating DNA sequences into the simian virus 40 (SV40) has been developed. SV40 recombinants carrying d(CG.GC)5, d(CA.GT)30 or d(CT.GA)22 insertions at either the TaqI site (position 4739) or the HpaII site (position 346) were obtained and the stability of the inserted DNA sequences studied. The palindromic potentially Z-DNA-forming d(CG.GC)n sequence was found to be highly unstable when compared to either d(CA.GT)n or d(CT.GA)n.
Casasnovas
J M
JM
Centro de Investigacion y Desarrollo, Consejo Superior de Investigaciones Cientificas, Universidad Politecnica de Catalunya, Barcelona, Spain.
Ellison
M J
MJ
Rodriguez-Campos
A
A
Azorin
F
F
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes