title: Superoxide-dependent oxidation of extracellular reducing agents by isolated neutrophils.
abstract: Incubation of stimulated neutrophils with sulfhydryl (RSH) compounds or ascorbic acid (ascorbate) results in rapid superoxide (O2-)-dependent oxidation of these reducing agents. Oxidation of RSH compounds to disulfides (RSSR) is faster than the rate of O2- production by the neutrophil NADPH-oxidase, whereas about one ascorbate is oxidized per O2-. Ascorbate is oxidized to dehydroascorbate, which is also oxidized but at a slower rate. Oxidation is accompanied by a large increase in oxygen (O2) uptake that is blocked by superoxide dismutase. Lactoferrin does not inhibit, indicating that ferric (Fe3+) ions are not required, and Fe3+-lactoferrin does not catalyze RSH or ascorbate oxidation. Two mechanisms contribute to oxidation: 1) O2- oxidizes ascorbate or reduced glutathione and is reduced to hydrogen peroxide (H2O2), which also oxidizes the reductants. O2- reacts directly with ascorbate, but reduced glutathione oxidation is mediated by the reaction of O2- with manganese (Mn2+). The H2O2-dependent portion of oxidation is mediated by myeloperoxidase-catalyzed oxidation of chloride to hypochlorous acid (HOCl) and oxidation of the reductants by HOCl. 2) O2- initiates Mn2+-dependent auto-oxidation reactions in which RSH compounds are oxidized and O2 is reduced. Part of this oxidation is due to the RSH-oxidase activity of myeloperoxidase. This activity is blocked by superoxide dismutase but does not require O2- production by the NADPH-oxidase, indicating that myeloperoxidase produces O2- when incubated with RSH compounds. It is proposed that an important role for O2- in the cytotoxic activities of phagocytic leukocytes is to participate in oxidation of reducing agents in phagolysosomes and the extracellular medium. Elimination of these protective agents allows H2O2 and products of peroxidase/H2O2/halide systems to exert cytotoxic effects.
authors.affiliation: Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
authors.forename: E L
authors.initials: EL
authors.lastname: Thomas
authors.forename: D B
authors.initials: DB
authors.lastname: Learn
authors.forename: M M
authors.initials: MM
authors.lastname: Jefferson
authors.forename: W
authors.initials: W
authors.lastname: Weatherred
mesh_terms: D001205:Ascorbic Acid / Q000097:blood; D002374:Catalase / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D003683:Dehydroascorbic Acid / Q000097:blood; D005978:Glutathione / Q000097:blood; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D008247:Lysosomes / Q000378:metabolism; D008345:Manganese / Q000097:blood; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D013439:Sulfhydryl Reagents / Q000378:metabolism*; D013481:Superoxides / Q000097:blood*
chemical_list: D002712:Chlorides; D013439:Sulfhydryl Reagents; D013481:Superoxides; D008345:Manganese; D006861:Hydrogen Peroxide; D002374:Catalase; D005978:Glutathione; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid