Molecular analysis of HLA-DP specificities HLA-DPw1, -DPw2 and -DPw4: DP beta chain heterogeneity correlates with PLT subtyping.
HLA-DP molecules were isolated from Epstein-Barr virus transformed B cell lines by immunoprecipitation with monoclonal antibody B7/21.2 and subsequently analysed by two-dimensional gel electrophoresis. The results obtained demonstrate that the HLA-DP molecules that can be isolated from cells positive for the HLA-DP specificities HLA-DPw1, -DPw2 and -DPw4 display DP beta chain isoelectric point differences, whereas no DP alpha chain polymorphism was observed. These results suggest that the PLT defined HLA-DP specificities (HLA-DPw1, -DPw2 and -DPw4) are probably DP beta chain structures.
Baas
E J
EJ
Department of Immunohaematology, University Hospital Leiden, The Netherlands.
Bontrop
R E
RE
Otting
N
N
Giphart
M J
MJ
D002460:Cell Line; D002472:Cell Transformation, Viral; D004586:Electrophoresis / Q000379:methods; D005782:Gels; D006681:HLA-D Antigens / Q000235:genetics*; D006682:HLA-DP Antigens / Q000235:genetics*; D004854:Herpesvirus 4, Human; D007137:Immunoglobulin alpha-Chains / Q000235:genetics; D008213:Lymphocyte Activation
D005782:Gels; D006681:HLA-D Antigens; D006682:HLA-DP Antigens; D007137:Immunoglobulin alpha-Chains