Purification of kappa-opioid receptor subtype to apparent homogeneity from frog brain.
Kappa-opioid receptor subtype was purified from digitonin solubilized preparation of frog brain membranes by hydrophobic and Sepharose 6B chromatography. 4800-fold purification over the membrane-bound receptors was achieved. The kappa receptor activity resides in a homo-oligomer of a single polypeptide of Mr 65,000.
Borsodi
A
A
Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged.
Khan
A
A
Simon
J
J
Benyhe
S
S
Hepp
J
J
Wollemann
M
M
Medzihradszky
K
K
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D011893:Rana esculenta; D011957:Receptors, Opioid / Q000302:isolation & purification* / Q000378:metabolism; D017473:Receptors, Opioid, kappa
D011957:Receptors, Opioid; D017473:Receptors, Opioid, kappa