Reacylation of platelet activating factor with eicosapentaenoic acid in fish-oil-enriched monkey neutrophils.
Platelet activating factor (PAF) is rapidly metabolized via a deacetylation: reacylation pathway which shows striking specificity for arachidonate at the sn-2 position of the 1-O-alkyl-2-acyl-GPC thus formed. We have now examined the effects of a diet enriched in fish oils on the metabolism of PAF and specificity for arachidonate in the reacylation reaction. [3H]PAF was incubated for various lengths of time with neutrophils from monkeys fed a control diet or one enriched in fish oils. The [3H]PAF added to the cell suspension was rapidly converted to 1-O-alkyl-2-acyl-GPC. Reverse-phase HPLC analysis of the acyl chains added at the sn-2 position revealed that arachidonate was the major fatty acid incorporated into the 1-O-alkyl-2-acyl-GPC formed by neutrophils from monkeys on the control diet. In contrast, both 1-O-alkyl-2-arachidonoyl-GPC and 1-O-alkyl-2-eicosapentaenoyl-GPC were formed by the fish-oil-enriched neutrophils. We also report on the fatty acid composition of neutrophil phospholipids during such a diet.
Chabot
M C
MC
Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
Schmitt
J D
JD
Bullock
B C
BC
Wykle
R L
RL
D000215:Acylation; D000818:Animals; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D005395:Fish Oils / Q000494:pharmacology*; D007700:Kinetics; D008252:Macaca fascicularis; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000378:metabolism*; D012016:Reference Values
D005395:Fish Oils; D010972:Platelet Activating Factor; D015118:Eicosapentaenoic Acid