The methylation state of the proviruses in avian sarcoma virus transformed chick and rat cells.
The endogenous viruses in the avian cells are not completely methylated, nor are the Schmidt-Ruppin RSV-D (SRD) proviruses in the infected cells completely unmethylated. Avian sarcoma proviruses integrated in rat transformed cloned cells are heavily methylated. In these cells, a region in the 3' end of the env gene is unmethylated in all the src-containing proviruses but not in the transformed defective (td) proviruses. A possible role for the hypomethylation of the 3' end of the env region is proposed.
Roguel
N
N
Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Moskowitz
H
H
Relevy
H
H
Hamburger
J
J
Kotler
M
M
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology; D008745:Methylation; D011533:Proviruses / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains
D004279:DNA, Viral; D004262:DNA Restriction Enzymes