Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: homology with protein phosphatase 2A.
A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in lambda gt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1-101 of the type-1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases.
Berndt
N
N
Department of Biochemistry, University of Dundee, Scotland.
Campbell
D G
DG
Caudwell
F B
FB
Cohen
P
P
da Cruz e Silva
E F
EF
da Cruz e Silva
O B
OB
Cohen
P T
PT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008969:Molecular Sequence Data; D009132:Muscles / Q000502:physiology; D010446:Peptide Fragments / Q000032:analysis; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D054648:Protein Phosphatase 2; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid
D010446:Peptide Fragments; D012333:RNA, Messenger; D004247:DNA; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2