title: cAMP-dependent activation of protein synthesis correlates with dephosphorylation of elongation factor 2.
abstract: The addition of 5 mM cAMP to a cell-free translation system from rabbit reticulocytes increases the rate of protein synthesis 3 5-fold. Lower concentrations of cAMP (0.005, 0.05 and 0.5 mM) have no effect on translation in this system. cAMP at all the concentrations tested stimulates the phosphorylation of the same pattern of polypeptides, while 5 mM cAMP additionally stimulates dephosphorylation of the 95 kDa polypeptide identified as elongation factor 2 (EF-2). Testing of the preparations of EF-2 with a different content of the phosphorylated form in poly(U)-directed poly(Phe) synthesis reveals that the EF-2 activity correlates with the fraction of non-phosphorylated EF-2. Thus cAMP-dependent activation of protein synthesis seems to be due to dephosphorylation of EF-2.
authors.affiliation: Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region.
authors.forename: A S
authors.initials: AS
authors.lastname: Sitikov
authors.forename: P N
authors.initials: PN
authors.lastname: Simonenko
authors.forename: E A
authors.initials: EA
authors.lastname: Shestakova
authors.forename: A G
authors.initials: AG
authors.lastname: Ryazanov
authors.forename: L P
authors.initials: LP
authors.lastname: Ovchinnikov
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002474:Cell-Free System; D000242:Cyclic AMP / Q000494:pharmacology*; D007526:Isoelectric Point; D007700:Kinetics; D010452:Peptide Biosynthesis; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000378:metabolism*; D010455:Peptides; D010766:Phosphorylation; D011072:Poly U / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
chemical_list: D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D010455:Peptides; D011072:Poly U; C012001:polyphenylalanine; D000255:Adenosine Triphosphate; D000242:Cyclic AMP