Cytochemical analysis of the reconstitution of endoplasmic reticulum after microinjection of rat liver microsomes into Xenopus oocytes.
Fragments of rough and smooth endoplasmic reticulum purified from rat liver were injected into Xenopus oocyte cytoplasm. Light and electron microscopy, cytochemistry, immunocytochemistry, and enzyme assay were employed to determine the fate of heterologous membranes in the host cytoplasm. The in vivo-incubated microsomes disappeared in a time-dependent manner. Within 3 hr, rough microsomes were replaced by flattened ER cisternae and smooth microsomes were replaced by a network of anastomosing tubules. Polyclonal antibodies against rat liver microsomes and protein A-gold complexes were applied to glycol methacrylate sections of microinjected oocytes. Specific labeling was observed over discrete rough and smooth ER cisternae 3 hr after microinjection. Endogenous ER was not labeled by this technique, and label was not observed when sections were treated with pre-immune antibodies. Diaminobenzidene cytochemistry of microinjected rat lacrimal gland microsomes revealed enzyme activity in heterologous microsomes after 3 hr of in vivo incubation. Control injected microsomes (inactivated by heat denaturation) became associated with autophagic vacuoles, coincident with changes in lysosomal activity. Freshly isolated un-denatured microsomes did not provoke changes in lysosomal activity, and glucose-6-phosphatase activity associated with microinjected membranes could be detected 21 hr after in vivo incubation. Since rat liver microsomes reconstitute after in vivo incubation into cytoplasmic structures resembling those from which they were derived, we conclude that the microinjected membrane fragments act as templates for their own three-dimensional organization.
Paiement
J
J
Departement d'Anatomie, Faculté de Médicine, Université de Montréal, Québec, Canada.
Kan
F W
FW
Lanoix
J
J
Blain
M
M
D000818:Animals; D004721:Endoplasmic Reticulum / Q000032:analysis / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D005958:Glucosephosphates / Q000032:analysis; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism; D008845:Microinjections; D008854:Microscopy, Electron; D008862:Microsomes, Liver / Q000032:analysis / Q000648:ultrastructure*; D009865:Oocytes / Q000032:analysis / Q000648:ultrastructure; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D051381:Rats; D014982:Xenopus laevis
D005958:Glucosephosphates; C020674:glycerol-2-phosphatase; D010744:Phosphoric Monoester Hydrolases