In vitro study of frog adrenal function--IX. Evidence against the involvement of lipoxygenase metabolites in the control of steroid production.
The possible role of arachidonic acid metabolites of the lipoxygenase pathway in the regulation of steroidogenesis was studied in vitro using perifused frog interrenal (adrenal) glands. Graded doses of arachidonic acid (10(-6)-10(-4)M) increased the production of corticosterone and aldosterone in a dose-dependent manner. In the presence of indomethacin (5 X 10(-6)M), the effect of arachidonic acid on steroid secretion was totally abolished. Nordihydroguaiaretic acid (NDGA: 10(-6)M), a lipoxygenase inhibitor, did not alter the spontaneous secretion of corticosteroids and did not impair the stimulatory effect of arachidonic acid. In the presence of NDGA, both ACTH and angiotensin II were still able to stimulate corticosteroid production. Our data support the view that arachidonic acid metabolites play an important role in the regulation of amphibian steroidogenesis. Moreover, the results show that the lipoxygenase pathway is not involved in the spontaneous secretion of corticosteroids and in angiotensin II- or ACTH-induced steroidogenesis.
Delarue
C
C
Groupe de Recherche en Endocrinologie Moléculaire, UA CNRS 650, Université de Rouen, Mont-Saint-Aignan, France.
Lihrmann
I
I
Feuilloley
M
M
Netchitaïlo
P
P
Idres
S
S
Leboulenger
F
F
Bélanger
A
A
Perroteau
I
I
Vaudry
H
H
D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D003345:Corticosterone / Q000096:biosynthesis* / Q000378:metabolism; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D007975:Leukotriene B4 / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism*; D009637:Masoprocol / Q000494:pharmacology; D011895:Rana ridibunda; D013189:SRS-A / Q000494:pharmacology
D001095:Arachidonic Acids; D013189:SRS-A; D007975:Leukotriene B4; D016718:Arachidonic Acid; D009637:Masoprocol; D008084:Lipoxygenase; D003345:Corticosterone; D007213:Indomethacin