title: Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae.
abstract: Saccharomyces cerevisiae contains two genes which encode cyclic AMP (cAMP) phosphodiesterase. We previously isolated and characterized PDE2, which encodes a high-affinity cAMP phosphodiesterase. We have now isolated the PDE1 gene of S. cerevisiae, which encodes a low-affinity cAMP phosphodiesterase. These two genes represent highly divergent branches in the evolution of phosphodiesterases. High-copy-number plasmids containing either PDE1 or PDE2 can reverse the growth arrest defects of yeast cells carrying the RAS2(Val-19) mutation. PDE1 and PDE2 appear to account for the aggregate cAMP phosphodiesterase activity of S. cerevisiae. Disruption of both PDE genes results in a phenotype which resembles that induced by the RAS2(Val-19) mutation. pde1- pde2- ras1- ras2- cells are viable.
authors.affiliation: Cold Spring Harbor Laboratory, New York 11724.
authors.forename: J
authors.initials: J
authors.lastname: Nikawa
authors.forename: P
authors.initials: P
authors.lastname: Sass
authors.forename: M
authors.initials: M
authors.lastname: Wigler
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D004252:DNA Mutational Analysis; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D006358:Hot Temperature; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D000242:Cyclic AMP; D004262:DNA Restriction Enzymes; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1