Interaction between PAF-acether and drugs that stimulate cyclic AMP in guinea-pig alveolar macrophages.
The PAF-acether (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine)-induced arachidonate release from alveolar macrophages was significantly reduced by prostaglandin E2 (PGE2) and by the beta-adrenoceptor agonist salbutamol. In addition, PAF-acether markedly reduced the increase in intracellular cyclic AMP (cAMP) concentrations induced by PGE2 and salbutamol. Our data indicate an inverse relationship between intracellular cAMP levels and free arachidonate availability in alveolar macrophages treated with PAF-acether. A rise in intracellular cAMP therefore represents an important alternative route for controlling the effects of PAF-acether and the resulting inflammatory alterations in the respiratory system.
Bachelet
M
M
Unité de Pharmacologie Cellulaire, INSERM 285, Paris, France.
Adolfs
M J
MJ
Masliah
J
J
Bereziat
G
G
Vargaftig
B B
BB
Bonta
I L
IL
D000420:Albuterol / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002448:Cell Adhesion; D000242:Cyclic AMP / Q000378:metabolism*; D015232:Dinoprostone; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D011458:Prostaglandins E / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism
D001095:Arachidonic Acids; D010972:Platelet Activating Factor; D011458:Prostaglandins E; D016718:Arachidonic Acid; D000242:Cyclic AMP; D015232:Dinoprostone; D000420:Albuterol