title: Identification and expression of a human cytomegalovirus glycoprotein with homology to the Epstein-Barr virus BXLF2 product, varicella-zoster virus gpIII, and herpes simplex virus type 1 glycoprotein H.
abstract: An open reading frame with the characteristics of a glycoprotein-coding sequence was identified by nucleotide sequencing of human cytomegalovirus (HCMV) genomic DNA. The predicted amino acid sequence was homologous with glycoprotein H of herpes simplex virus type 1 and the homologous protein of Epstein-Barr virus (BXLF2 gene product) and varicella-zoster virus (gpIII). Recombinant vaccinia viruses that expressed this gene were constructed. A glycoprotein of approximately 86 kilodaltons was immunoprecipitated from cells infected with the recombinant viruses and from HCMV-infected cells with a monoclonal antibody that efficiently neutralized HCMV infectivity. In HCMV-infected MRC5 cells, this glycoprotein was present on nuclear and cytoplasmic membranes, but in recombinant vaccinia virus-infected cells it accumulated predominantly on the nuclear membrane.
authors.affiliation: Department of Pathology, University of Cambridge, England.
authors.forename: M P
authors.initials: MP
authors.lastname: Cranage
authors.forename: G L
authors.initials: GL
authors.lastname: Smith
authors.forename: S E
authors.initials: SE
authors.lastname: Bell
authors.forename: H
authors.initials: H
authors.lastname: Hart
authors.forename: C
authors.initials: C
authors.lastname: Brown
authors.forename: A T
authors.initials: AT
authors.lastname: Bankier
authors.forename: P
authors.initials: P
authors.lastname: Tomlinson
authors.forename: B G
authors.initials: BG
authors.lastname: Barrell
authors.forename: T C
authors.initials: TC
authors.lastname: Minson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D014645:Herpesvirus 3, Human / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C044200:glycoprotein H, herpes simplex virus type 1; C047840:glycoprotein gp3, varicella-zoster virus