Inhibition of cyclic AMP-dependent induction of ornithine aminotransferase by simple carbohydrates in cultured hepatocytes.
Glucose administration inhibits the induction of ornithine aminotransferase (OAT) in both the whole animal and cultured hepatocytes. We have examined the ability of several hexoses and related molecules to inhibit the cAMP-dependent induction of OAT in primary cultures of adult rat hepatocytes. The hexoses (D-glucose, fructose, sorbitol, sorbose, and mannose) that were effective as inhibitors of OAT induction also resulted in accumulation of lactate in the culture medium, although lactate itself was not effective as an inhibitor. The hexoses and related 6-carbon structures (galactose, L-glucose, 2-deoxyglucose, 3-O-methylglucose, rhamnose, mannitol, and inositol) that were not effective as inhibitors of OAT induction did not result in accumulation of lactate in the culture medium. These results suggest that the carbohydrate repression of hepatic OAT requires metabolism of the carbohydrate by the liver cell. Upon addition to the culture medium of several compounds related to carbohydrate metabolism, many (ribose, xylitol, dihydroxyacetone, and glycerol) exhibited an inhibitory effect, with glycerol exhibiting the greatest effect. Fructose and glycerol inhibit OAT induction in the presence of 2-deoxyglucose, suggesting that the inhibitory effect of nonglucose carbohydrates is not occurring through conversion to glucose. The carbon sources observed to be most effective as inhibitors of OAT induction (glycerol, fructose, sorbitol, and sorbose result in more than 90% inhibition at 25 mM) all enter the glycolytic pathway at the triosephosphate level. The mechanism of the inhibitory effect of simple carbohydrates on OAT induction is not known but may involve an increase in certain glycolytic intermediates. Glucose and the related carbon sources exert their effect by inhibiting the cAMP-dependent increase in OAT synthesis. The cAMP-dependent increase in OAT mRNA was inhibited by fructose. These findings suggest that the carbohydrate inhibition of the cAMP-dependent increase in OAT synthesis occurs at a pretranslational level.
Merrill
M J
MJ
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Pitot
H C
HC
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology*; D004098:Dihydroxyacetone / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D009953:Ornithine-Oxo-Acid Transaminase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013012:Sorbitol / Q000494:pharmacology; D013013:Sorbose / Q000494:pharmacology; D000637:Transaminases / Q000096:biosynthesis*
D002241:Carbohydrates; D012333:RNA, Messenger; D005632:Fructose; D013012:Sorbitol; D003994:Bucladesine; D000242:Cyclic AMP; D000637:Transaminases; D009953:Ornithine-Oxo-Acid Transaminase; D005947:Glucose; D013013:Sorbose; D004098:Dihydroxyacetone; D005990:Glycerol