[Distribution of the tempo-dichlorotriazine spin label in the immunoglobulin molecule].
Spin-label 14C-TEMPO-dichlorotriazine distribution in Fab- and Fc-fragments of monoclonal myeloma immunoglobulin IgG (lambda) 1 Bel, in their heavy and light chains, and tryptic fragments of chains was investigated. Radio-active spin label in proteolytic Fab- and Fc-fragments, in heavy and light chains, and in separate peptides was detected. 3 cm and 2 mm band ESR spectra of spin-labeled IgG in water solutions was studied. It was shown that the models of highly anisotropic motion, isotropic motion and slow isotropic motion of spin label around globule or the description of experimental ESR spectra of TEMPO-dichlorotriazine spin-labeled immunoglobulins do not work.
Bondarev
G N
GN
Isaev-Ivanov
V V
VV
Isaeva-Ivanova
L S
LS
Kleĭner
A R
AR
Krasotskaia
G I
GI
D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis*; D013113:Spin Labels; D014227:Triazines
D003497:Cyclic N-Oxides; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D013113:Spin Labels; D014227:Triazines; C003959:TEMPO
Raspredelenie spinovoĭ metki tempo-dikhlorotriazina po molekule immunoglobulina.