title: [Development of rational methods for purifying restriction endonucleases].
abstract: The work deals with the search for rational schemes of the purification of endonucleases, suitable for the isolation of different enzymes. The scheme using the combination of Blue Sepharose and phosphocellulose has proved to be most universal. The expediency of starting the purification of Haemophilus enzymes on biogel A-0.5m has been established.
authors.forename: N V
authors.initials: NV
authors.lastname: Tsvetkova
mesh_terms: D001419:Bacteria / Q000201:enzymology; D002482:Cellulose / Q000031:analogs & derivatives; D002852:Chromatography, Ion Exchange / Q000379:methods; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D005818:Genetic Engineering / Q000379:methods; D012685:Sepharose / Q000031:analogs & derivatives
chemical_list: C029483:Blue-Sepharose; D002482:Cellulose; D012685:Sepharose; C008881:phosphocellulose; D004262:DNA Restriction Enzymes
vernacular_title: Razrabotka ratsional'nykh metodov ochistki restriktsionnykh éndonukleaz.