Estrogen-dependent in vitro transcription from the vitellogenin promoter in liver nuclear extracts.
One approach to analyzing the molecular mechanisms of gene expression in vivo is to reconstitute these events in cell-free systems in vitro. Although there is some evidence for tissue-specific transcription in vitro, transcriptionally active extracts that mimic a steroid hormone-dependent enhancement of transcription have not been described. In the study reported here, nuclear extracts of liver from the frog Xenopus laevis were capable of estrogen-dependent induction of a homologous vitellogenin promoter that contained the estrogen-responsive element.
Corthésy
B
B
Institut de Biologie animale, Université de Lausanne, Switzerland.
Hipskind
R
R
Theulaz
I
I
Wahli
W
W
D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D015500:Chloramphenicol O-Acetyltransferase; D004274:DNA, Recombinant; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects*; D014819:Vitellogenins / Q000235:genetics*; D014982:Xenopus laevis
D004274:DNA, Recombinant; D014819:Vitellogenins; D004958:Estradiol; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D012319:RNA Polymerase II