Structural alteration in the MYB protooncogene and deletion within the gene encoding alpha-type protein kinase C in human melanoma cell lines.
A correlative study was done to determine possible relationships between nonrandom aberrations in chromosomes 1, 6, and 7 occurring in human cutaneous malignant melanoma and the structure of oncogenes as well as specific genes encoding growth factors and growth factor receptors. Thirty cell lines derived from primary or metastatic melanomas of 28 patients were analyzed by Southern blotting with nick-translated probes for 28 different genes, some of which map near frequent chromosomal breakpoints observed in melanoma. An alteration in the MYB protooncogene was observed in a cell line derived from a primary melanoma in the vertical growth phase, which correlated with a 6q22 chromosomal abnormality. Another primary melanoma cell line had a cytogenetically undetected tumor-specific deletion within the gene for alpha-type protein kinase C. Polymorphic alleles for the genes encoding the epidermal growth factor receptor and alpha-type protein kinase C were also observed.
Linnenbach
A J
AJ
Wistar Institute, Philadelphia, PA 19104.
Huebner
K
K
Reddy
E P
EP
Herlyn
M
M
Parmiter
A H
AH
Nowell
P C
PC
Koprowski
H
H
D002460:Cell Line; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002896:Chromosomes, Human, Pair 6; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics; D005796:Genes; D006239:Haplotypes; D006801:Humans; D008545:Melanoma / Q000201:enzymology / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011493:Protein Kinase C / Q000235:genetics*; D011519:Proto-Oncogenes
D004273:DNA, Neoplasm; D011493:Protein Kinase C; D004262:DNA Restriction Enzymes