Relationship between the electrochemical proton gradient and respiration: pyruvate and l-palmitoylcarnitine as hydrogen donors for rat heart mitochondria.
The electrochemical proton gradient (delta/uH+) and the rate of respiration were determined as function of the work load at conditions where isolated rat heart mitochondria utilize pyruvate and/or l-palmitoyl-carnitine as hydrogen donors. Delta/uH+ was calculated from the distribution of 86Rb+ (delta psi marker) and [14C]-dimethyloxazolidine-2,4-dione (delta pH marker) between the mitochondrial matrix space and the medium. A transition from l-palmitoylcarnitine to pyruvate utilization did not change the rate of hydrogen supply or the mitochondrial energy state.
Schönfeld
P
P
Institut für Biochemie der Medizinischen Akademie, Magdeburg.
Petzold
D
D
D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives*; D004563:Electrochemistry; D004734:Energy Metabolism; D005260:Female; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008929:Mitochondria, Heart / Q000378:metabolism*; D010101:Oxygen Consumption; D010172:Palmitoylcarnitine / Q000378:metabolism*; D011522:Protons; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid; D051381:Rats
D011522:Protons; D011773:Pyruvates; D010172:Palmitoylcarnitine; D019289:Pyruvic Acid; D002331:Carnitine