Multiple proteins encoded within the urease gene complex of Proteus mirabilis.
Chromosomal DNA fragments from a uropathogenic isolate of Proteus mirabilis were inserted into the cosmid vector pHC79 to construct a genomic library in Escherichia coli HB101. A urease-positive recombinant cosmid, designated pSKW1, was recovered. Sequential recombinant manipulation of pSKW1 yielded a 10.2-kilobase plasmid, designated pSKW4, which encoded three urease isozymes with electrophoretic mobilities identical to those of the donor P. mirabilis strain. Plasmid pSKW4 gene sequences encode seven proteins designated 68K (apparent molecular weight, of 68,000), 28K, 25K, 22.5K, 18.5K, 7.5K, and 5.2K within the limits of the urease gene complex. Insertion mutations in genes encoding the 68K, 28K, 25K, 22.5K, 7.5K, and 5.2K proteins resulted in complete or partial (22.5K) loss of urease activity. There was no reduction in urease activity when the gene encoding the 18.5K protein was inactivated.
Walz
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Department of Microbiology and Immunology, Baylor College of Medicine, Texas Medical Center, Houston 77030.
Wray
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Hull
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Hull
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D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D014176:Protein Biosynthesis; D011513:Proteus mirabilis / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D014510:Urease / Q000235:genetics*; D014552:Urinary Tract Infections / Q000382:microbiology
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes; D014510:Urease