Selective spin-labelling of an N-acetylneuraminic acid residue in the Fab-region oligosaccharide of immunoglobulin M.
A method is proposed for spin-labelling using 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl, the N-acetylneuraminic acid residue within the Fab-region oligosaccharide of immunoglobulin M (IgM). The selectivity of spin-labelling was achieved by the controlled periodate oxidation of the oligosaccharide groups of the IgM prior to the spin-labelling. The spin-labelled IgM prepared in this way may be useful for the studies of dynamics of the IgM structure, particularly upon antigen binding.
Timofeev
V P
VP
Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow.
Lapuk
V A
VA
Tchukhrova
A I
AI
Arutiunian
A E
AE
D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides; D012794:Sialic Acids; D013113:Spin Labels
D003497:Cyclic N-Oxides; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D009844:Oligosaccharides; D012794:Sialic Acids; D013113:Spin Labels; C015527:4-amino-TEMPO; D019158:N-Acetylneuraminic Acid