Developmental and regional changes of mRNA for a cerebellar protein (spot 35) in the rat brain.
Spot 35 protein is a cerebellar Ca-binding protein. Because Southern blot analysis showed evidence for the nucleotide sequence of spot 35 protein cDNA in the rat genome, we applied cDNA to quantitate the mRNA of rat spot 35 protein. The size of this mRNA was about 1,900 nucleotides in length, and that of mRNA from bovine cerebellum was larger. We also examined the developmental changes and regional distribution of spot 35 protein mRNA in rat brains by dot-blot analysis using cDNA as a probe. During postnatal days 1-20, a rapid increase of mRNA levels was observed. Further, the level of mRNA for spot 35 protein was found in the cerebellum and was negligible in the cerebral cortex, striatum, and hippocampus.
Yamakuni
T
T
Department of Neuropharmacology, Niigata University, Japan.
Kuwano
R
R
Araki
K
K
Usui
H
H
Inoue
Y
Y
Takahashi
Y
Y
D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D064092:Calbindin 1; D064026:Calbindins; D002135:Calcium-Binding Proteins; D002417:Cattle; D002531:Cerebellum / Q000254:growth & development* / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D006624:Hippocampus / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D064030:S100 Calcium Binding Protein G; D014018:Tissue Distribution
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G; D004247:DNA; D004262:DNA Restriction Enzymes