Binding of ellipticine base and ellipticinium cation to calf-thymus DNA. A thermodynamic and kinetic study.
The acid-basic properties of ellipticine have been re-estimated. The apparent pK of protonation at 3 microM drug concentration is 7.4 +/- 0.1. The ellipticine free base (at pH 9, I = 25 mM) intercalates into calf-thymus DNA with an affinity constant of 3.3 +/- 0.2 X 10(5) M-1, and a number of binding sites per phosphate of 0.23. The ellipticinium cation (pH 5, I = 25 mM) binds also to DNA with a constant of 8.3 +/- 0.2 x 10(5) M-1 and at a number of binding sites (n = 0.19). It is postulated that the binding of the drug to DNA at pH 9 is driven by hydrophobic and/or dipolar effects. Even at pH 5, where ellipticine exists as a cation, it is thought that the hydrophobic interaction is the main contribution to binding. The neutral and cationic forms share common binding within DNA sites but yield to structurally different complexes. The free base has 0.04 additional specific binding sites per phosphate. As determined from temperature-jump experiments, the second-order rate constant of the binding of the free base (pH 9) is 3.4 x 10(7) M-1 s-1 and the residence time of the base within the DNA is 8 ms. The rate constant for the binding of the ellipticinium cation is 9.8 x 10(7) M-1 s-1 when it is assumed that drug attachment occurs via a pathway in which the formation of an intermediate ionic complex is not involved (competitive pathway).
Dodin
G
G
Groupe de Dynamique des Interactions Macromoleculaires, Institut de Topologie et de Dynamique des Systémes, Paris, France.
Schwaller
M A
MA
Aubard
J
J
Paoletti
C
C
D000470:Alkaloids / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D004611:Ellipticines / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011522:Protons; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013816:Thermodynamics; D013950:Thymus Gland; D014783:Viscosity
D000470:Alkaloids; D004611:Ellipticines; D011522:Protons; C027211:2-methylellipticinium; D004247:DNA