Isolation of cathepsin D using three-phase partitioning in t-butanol/water/ammonium sulfate.
A 6-h procedure for the isolation of bovine cathepsin D is described. The procedure involves essentially only two steps; three-phase partitioning in t-butanol/water/ammonium sulfate followed by affinity chromatography on pepstatin-agarose. The major advantage of this new method over previous methods is the greatly reduced time required to obtain comparably pure cathepsin D.
Jacobs
G R
GR
Department of Biochemistry, University of Natal, Pietermaritzburg, South Africa.
Pike
R N
RN
Dennison
C
C
D000645:Ammonium Sulfate; D000818:Animals; D000440:Butanols; D002402:Cathepsin D / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D013154:Spleen / Q000201:enzymology; D014867:Water; D020002:tert-Butyl Alcohol
D000440:Butanols; D014867:Water; D002402:Cathepsin D; D020002:tert-Butyl Alcohol; D000645:Ammonium Sulfate