The Drosophila ets-2 gene: molecular structure, chromosomal localization, and developmental expression.
The cellular homologs of the ets gene from the avian erythroblastosis retrovirus E26 have been studied in chickens, humans, mice, and cats. In this report a further evolutionary step is taken by isolating and characterizing a Drosophila ets-related genomic clone. Sequence analysis of this clone has shown it to contain the 3' end of the v-ets gene, called ets-2, corresponding to the last two exons of chicken ets. The predicted amino acid sequence was found to have over 90% homology when compared to that of v-ets. This is the highest level of conservation observed for any previously characterized Drosophila oncogene homolog. Expression of the ets-2 gene occurs throughout development, but is highest during the embryonic and pupal stages. By in situ hybridization, the ets-2 chromosomal position was determined to be 58A/B which corresponds to no known phenotypic mutant. As this is a highly conserved gene, the Drosophila model system should prove useful for the determination of the ets gene function.
Pribyl
L J
LJ
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701-1013.
Watson
D K
DK
McWilliams
M J
MJ
Ascione
R
R
Papas
T S
TS
D001355:Alpharetrovirus / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D005091:Exons; D005786:Gene Expression Regulation; D007438:Introns; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D011679:Pupa / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes