Absence of allogeneic T cell response to human insulin-secreting cells following long-term culture.
Lymphocyte proliferative responses (3H-Thymidine uptake) were studied in mixed culture combinations of peripheral blood lymphocytes as responder cells and human insulinoma cells as stimulator cells (MILR). Influence of culture time on the ability of insulinoma cells to stimulate allogeneic T cell proliferation was examined. Crude cell suspensions initiated a strong lymphoproliferative response with a stimulation index (SI) that ranged between 3 and 7, whereas insulinoma cells did not stimulate allogeneic T cells after one month of culture. Expression of cell surface determinants coded by the major histocompatibility complex (MHC) was evaluated simultaneously by indirect immunofluorescence using monoclonal antibodies to class I shared-determinant or class II molecules. Human insulinoma cells expressed class I but not class II molecules. Crude insulinoma cell suspensions were found to be contaminated by 2% of DR+ cells from nonislet components. It is postulated that loss of these DR+ lymphoreticular cells with culture time resulted in absence of immune recognition and lymphoproliferative response. These results emphasize the need of culturing human islet cells prior to transplantation in order to reduce cell immunogenicity.
Demidem
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INSERM U209 Edouard Herriot Hospital, Lyon, France.
Thivolet
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CH
D007516:Adenoma, Islet Cell / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007340:Insulinoma / Q000276:immunology*; D007515:Islets of Langerhans / Q000276:immunology*; D016381:Islets of Langerhans Transplantation; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D006680:HLA Antigens