Relaxation of smooth muscle by cardiodilatin/atrial natriuretic peptide is inhibited by cAMP-dependent phosphorylation.
Cardiodilatins/atrial natriuretic peptides (CDD/ANP) exhibit a common amino acid sequence: Arg101-Arg102-Ser103-Ser104. Cyclic AMP-dependent phosphorylation of Ser104 of atrial peptides with [gamma-32P]ATP enables rapid identification of cardiac hormones. The biological activity of in vitro phosphorylated cardiodilatin (CDD-28/alpha-hANP) is dramatically altered compared to the unphosphorylated peptide: the vaso-relaxant effect of cardiodilatin 28 is inhibited upon phosphorylation.
Gagelmann
M
M
Institute of Anatomy III, University of Heidelberg, FRG.
Hock
D
D
Forssmann
W G
WG
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects*; D009124:Muscle Proteins / Q000494:pharmacology*; D009126:Muscle Relaxation / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits
D009124:Muscle Proteins; D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor; C040651:cardiodilatin; C054396:atrial natriuretic factor 88; D000242:Cyclic AMP; D011494:Protein Kinases