title: Role of hemolysin for the intracellular growth of Listeria monocytogenes.
abstract: Listeria monocytogenes insertion mutants defective in hemolysin production were generated using the conjugative transposons Tn916 and Tn1545. All of the nonhemolytic mutants (hly-) lacked a secreted 58-kD polypeptide, presumedly hemolysin, and were avirulent in a mouse model. An intracellular multiplication assay was established in monolayers of mouse bone marrow-derived macrophages, the J774 macrophage-like cell line, the CL.7 embryonic mouse fibroblast cell line, and the Henle 407 human epithelial cell line. The hly+ strain grew intracellularly in all of the tissue culture cells with a doubling time of approximately 60 min. In contrast, the hly- mutants failed to grow in the murine-derived tissue culture cells, but retained the ability to grow in the human tissue culture cells examined. Hemolytic-positive revertants were selected after passage of the hly- mutants through monolayers of J774 cells. In each case, the hemolytic revertants possessed the 58-kD polypeptide, were capable of intracellular growth in tissue culture monolayers and were virulent for mice.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Washington University, St. Louis, Missouri 63110.
authors.forename: D A
authors.initials: DA
authors.lastname: Portnoy
authors.forename: P S
authors.initials: PS
authors.lastname: Jacks
authors.forename: D J
authors.initials: DJ
authors.lastname: Hinrichs
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D004251:DNA Transposable Elements; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D005347:Fibroblasts / Q000382:microbiology; D006460:Hemolysin Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008089:Listeria monocytogenes / Q000166:cytology* / Q000235:genetics; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D009928:Organ Specificity; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D006460:Hemolysin Proteins