title: Primary structure of rat chromogranin A and distribution of its mRNA.
abstract: The primary structure of rat chromogranin A has been deduced from a rat adrenal cDNA clone. A comparison of rat and bovine chromogranin A reveals similar features: clusters of polyglutamic acid, similar amino acid composition, position of seven of 10 pairs of basic amino acids, identical placement of the only two cysteine residues, a highly conserved N- and C-terminus, and a sequence homologous to porcine pancreastatin 1-49 [(1986) Nature 324, 476-478]. Unique features of rat chromogranin A are an eicosaglutamine sequence and two potential N-linked glycosylation sites. Chromogranin A mRNA is detectable in adrenal medulla, anterior pituitary, cerebral cortex, and hippocampus, as well as tumor cell lines derived from pancreas, pituitary, and adrenal medulla.
authors.affiliation: Unit on Molecular and Cellular Neurobiology, NIMH, Bethesda, MD 20892.
authors.forename: A
authors.initials: A
authors.lastname: Iacangelo
authors.forename: H
authors.initials: H
authors.lastname: Okayama
authors.forename: L E
authors.initials: LE
authors.lastname: Eiden
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D053379:Chromogranin A; D002864:Chromogranins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
chemical_list: D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C506153:chromogranin A, rat; D004247:DNA; D004262:DNA Restriction Enzymes