Absence of some truncated genes in the amphidiploid Nicotiana tabacum.
As an initial step towards understanding how a multigene family evolves after an interspecific hybridization and subsequent chromosomal doubling, genomic Southern blots of three related species were compared: Nicotiana tabacum (the progeny), and Nicotiana sylvestris and Nicotiana tomentosiformis (the progenitors). Genomic restriction fragments generated by two endonucleases were hybridized with a cDNA of the small subunit of ribulose bisphosphate carboxylase from N. sylvestris. The restriction pattern of the DNA of the progenitors revealed considerable polymorphism of restriction-fragment lengths. All the fragments in N. tabacum, except one, have a corresponding fragment in one of the progenitors. Some fragments present in the parents were absent from the progeny: they may correspond to truncated genes consisting of only part of the 3' portion of the gene.
Jamet
E
E
Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg, France.
Durr
A
A
Fleck
J
J
D004262:DNA Restriction Enzymes; D004171:Diploidy; D005796:Genes; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D013045:Species Specificity; D014026:Nicotiana / Q000235:genetics
D004262:DNA Restriction Enzymes