Transduction in Bacillus stearothermophilus.
Temperate and virulent bacteriophages isolated from soil were shown to carry out generalized transduction of Bacillus stearothermophilus NUB36. A transducing frequency of 1 X 10(-5) to 7 X 10(-4) was obtained for temperate phages TP-42 and TP-56. The transducing frequency for virulent phage TP-68 was two to three orders of magnitude lower. Cotransfer analysis with the three phages showed that hom-1 is linked to thr-1 and that gly-1 is linked to his-1.
Welker
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NE
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.
D001435:Bacteriophages; D004262:DNA Restriction Enzymes; D001411:Geobacillus stearothermophilus / Q000235:genetics*; D012988:Soil Microbiology; D014161:Transduction, Genetic
D004262:DNA Restriction Enzymes