Latency of acid hydrolases in rat kidney cortex.
1. Some lysosomal populations in the rat kidney cortex appear to be mechanically weak and are readily disrupted by gentle homogenization, while other populations remain intact even after repeated homogenization. 2. Lysosomes in the rat kidney cortex appear to be resistant to hypertonic media but are readily disrupted under hypotonic conditions. 3. Lysosomes in rat kidney cortex are readily disrupted when incubated in isotonic sucrose at 37 degrees C. 4. Measurement of total and free activity of three acid hydrolases: N-acetyl-beta-D-glucosaminidase (NAG), acid beta-galactosidase and acid beta-glycerophosphatase, indicates that the latency of these enzymes is relatively low in the homogenate (10-29%) and the ML-fraction (14-42%), but high (60-95%) in the purified large lysosomes (protein droplets). 5. The latency of purified small lysosomes is relatively lower (30-60%) than that of large lysosomes, suggesting that small lysosome populations are relatively permeable to the acid hydrolase substrates. 6. Latency variations of acid hydrolases amongst subcellular fractions appear to reflect the heterogeneity of lysosomal populations present in the kidney cortical homogenate.
Haga
H J
HJ
Medical Department A, University of Bergen, Haukeland Sykehus, Norway.
Andersen
K J
KJ
Dobrota
M
M
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D002499:Centrifugation, Density Gradient; D006867:Hydrolases / Q000378:metabolism*; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000201:enzymology*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D001616:beta-Galactosidase / Q000378:metabolism
D006867:Hydrolases; C020674:glycerol-2-phosphatase; D010744:Phosphoric Monoester Hydrolases; D001616:beta-Galactosidase; D000118:Acetylglucosaminidase