title: Role of atrial natriuretic peptide and urinary cGMP in the natriuretic and diuretic response to central hypervolemia in normal human subjects.
abstract: The response of plasma atrial natriuretic peptide (ANP) and urinary cGMP excretion to central hypervolemia induced by water immersion was assessed twice in five healthy male subjects, once while immersed in water to the neck for 3 h and again on a control day. Plasma ANP and urinary cGMP were measured by radioimmunoassay. Compared with the control day, overall change in plasma ANP on the immersion day was significant (p less than 0.05). In response to water immersion, plasma ANP increased from a base-line level of 13.2 +/- 3.1 (mean +/- SEM) to 24.2 +/- 5.5 pg/mL by 0.5 h of immersion and was sustained at that level throughout the immersion period. Plasma ANP returned to the base-line level at 1 h postimmersion. Urinary cGMP excretion increased significantly by 1 h of immersion and was sustained at that level throughout water immersion and 1 h postimmersion (p less than 0.05). During water immersion urine flow, urinary sodium and potassium excretion, free water clearance, and osmolar clearance increased while plasma renin activity, serum aldosterone, and blood pressure fell; all changes were significant (p less than 0.05). Creatinine clearance and hematocrit did not show any significant changes. These data suggest that an increase in plasma ANP may contribute to the natriuretic and diuretic response to central hypervolemia, and that the measurement of urinary cGMP may be a valuable marker of ANP biological responsiveness.
authors.affiliation: Department of Medicine, University of Toronto, Ont., Canada.
authors.forename: W M
authors.initials: WM
authors.lastname: Leung
authors.forename: A G
authors.initials: AG
authors.lastname: Logan
authors.forename: P J
authors.initials: PJ
authors.lastname: Campbell
authors.forename: T E
authors.initials: TE
authors.lastname: Debowski
authors.forename: S B
authors.initials: SB
authors.lastname: Bull
authors.forename: P Y
authors.initials: PY
authors.lastname: Wong
authors.forename: L M
authors.initials: LM
authors.lastname: Blendis
authors.forename: K L
authors.initials: KL
authors.lastname: Skorecki
mesh_terms: D000328:Adult; D000450:Aldosterone / Q000097:blood; D009320:Atrial Natriuretic Factor / Q000502:physiology*; D001794:Blood Pressure; D001810:Blood Volume; D006152:Cyclic GMP / Q000652:urine*; D004231:Diuresis; D006801:Humans; D007101:Immersion / Q000503:physiopathology; D008297:Male; D009318:Natriuresis; D012016:Reference Values; D012083:Renin / Q000097:blood
chemical_list: D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D012083:Renin; D006152:Cyclic GMP