Molecular cloning and sequencing of a cDNA for a carbohydrate binding receptor unique to rat Kupffer cells.
cDNA comprising the entire length of the rat Kupffer cell receptor (Mr = 88,000 and 77,000) for carbohydrates with an affinity for fucose and galactose was isolated, and its nucleotide sequence was determined. Receptor cDNA encoded a protein containing 550 amino acid residues with a Mr = 61,104. This Mr was consistent with the size of the deglycosylated receptor which was found to contain two polypeptides by gel electrophoresis with Mr = 58,000 and 52,000, respectively. Edman degradation of the receptor yielded a sequence which corresponded to amino acid residues 83-104 of the sequence derived from the cDNA. This confirmed that the cDNA which had been isolated corresponded to mRNA for the receptor and suggested that the smaller polypeptide in receptor preparations arises by proteolysis of the intact receptor. Amino acid composition of the receptor was nearly identical to that predicted by the cDNA. The Kupffer cell receptor was found to be homologous to other carbohydrate binding proteins including the hepatic receptors with different binding specificities. The Kupffer cell receptor also contained a series of 18 contiguous, homologous sequences with an average length of 14 residues.
Hoyle
G W
GW
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
Hill
R L
RL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D007728:Kupffer Cells / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics*
D011956:Receptors, Cell Surface; C039707:galactose receptor; D004247:DNA