Multilineage hematopoietic disorders induced by transplantation of bone marrow cells expressing the v-fms oncogene.
Mouse bone marrow cells infected with a helper-free retrovirus containing v-fms were engrafted into lethally irradiated mice. Dominant provirus-positive clones emerged in the spleens of some recipients within 1 month. When spleen cells were transplanted into lethally irradiated secondary recipients, clonal erythroleukemias or B cell lymphomas expressing the v-fms-coded glycoprotein developed. Other secondary recipients repopulated by "unmarked" progenitor cells or by cryptic provirus-positive precursors present in the spleens of the same donor mice did not develop disease; thus cells expressing v-fms did not invariably have a proliferative advantage after transplantation. Several primary engrafted recipients developed myeloproliferative disorders that were provirus-positive without evidence of clonality. Although expression of the c-fms product (CSF-1 receptor) is normally restricted to cells of the mononuclear phagocyte series, the v-fms-coded glycoprotein can contribute to proliferative abnormalities of multiple hematopoietic lineages.
Heard
J M
JM
Department of Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Roussel
M F
MF
Rettenmier
C W
CW
Sherr
C J
CJ
D000818:Animals; D016026:Bone Marrow Transplantation; D002472:Cell Transformation, Viral; D005814:Genes, Viral; D004915:Leukemia, Erythroblastic, Acute / Q000209:etiology* / Q000235:genetics; D008223:Lymphoma / Q000209:etiology* / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D051379:Mice; D009196:Myeloproliferative Disorders / Q000209:etiology* / Q000235:genetics; D016281:Oncogene Protein gp140(v-fms); D009857:Oncogenes; D011828:Radiation Chimera; D012190:Retroviridae / Q000472:pathogenicity*; D012191:Retroviridae Proteins / Q000235:genetics / Q000502:physiology*; D005253:Sarcoma Viruses, Feline / Q000235:genetics / Q000472:pathogenicity*; D013154:Spleen / Q000637:transplantation; D014162:Transfection
D008565:Membrane Proteins; D016281:Oncogene Protein gp140(v-fms); D012191:Retroviridae Proteins