Alteration of vesicular stomatitis virus L and NS proteins by uv irradiation: implications for the mechanism of host cell shut-off.
When purified, [35S]methionine-labeled vesicular stomatitis virus  (VSV) was exposed to ultraviolet light, an irradiation-induced change in the viral proteins was detected by SDS-polyacrylamide gel electrophoresis and immunoblotting. With dose of uv irradiation in the same range as that required to inactivate VSV leader RNA, a loss occurred in the bands corresponding to the L and NS proteins concomitant with the appearance of several new bands of radioactivity throughout the gel. This alteration of viral proteins correlated with the loss of ability of the virus to inhibit host macromolecular synthesis. In light of these results, the role that has been ascribed to the VSV leader RNA in VSV-mediated host shut-off needs to be reevaluated.
Whitaker-Dowling
P
P
Department of Microbiology, Biochemistry and Molecular Biology, School of Medicine, University of Pittsburgh, Pennsylvania 15261.
Youngner
J S
JS
D000818:Animals; D002213:Capsid / Q000502:physiology / Q000528:radiation effects*; D007739:L Cells / Q000378:metabolism; D051379:Mice; D014176:Protein Biosynthesis; D012324:RNA-Dependent RNA Polymerase; D014466:Ultraviolet Rays; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology / Q000528:radiation effects*; D014758:Viral Core Proteins / Q000502:physiology / Q000528:radiation effects*; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000502:physiology / Q000528:radiation effects*
D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C040530:L protein, vesicular stomatitis virus; D012324:RNA-Dependent RNA Polymerase