HIV infection is blocked in vitro by recombinant soluble CD4.
The T-cell surface glycoprotein, CD4 (T4), acts as the cellular receptor for human immunodeficiency virus, type 1 (HIV-1), the first member of the family of viruses that cause acquired immunodeficiency syndrome. HIV recognition of CD4 is probably mediated through the virus envelope glycoprotein (gp120) as shown by co-immunoprecipitation of CD4 and gp120 (ref.5) and by experiments using recombinant gp120 as a binding probe. Here we demonstrate that recombinant soluble CD4(rsT4) purified from the conditioned medium of a stably transfected Chinese hamster ovary cell line is a potent inhibitor of both virus replication and virus-induced cell fusion (syncytium formation). These results suggest that rsT4 is sufficient to bind HIV, and that it represents a potential anti-viral therapy for HIV infection.
Fisher
R A
RA
Biogen Research Corporation, Cambridge, Massachusetts 02142.
Bertonis
J M
JM
Meier
W
W
Johnson
V A
VA
Costopoulos
D S
DS
Liu
T
T
Tizard
R
R
Walker
B D
BD
Hirsch
M S
MS
Schooley
R T
RT
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D001667:Binding, Competitive; D002459:Cell Fusion; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006678:HIV / Q000502:physiology*; D015699:HIV Envelope Protein gp120; D010053:Ovary; D010446:Peptide Fragments / Q000378:metabolism; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D012191:Retroviridae Proteins / Q000378:metabolism; D012995:Solubility; D014779:Virus Replication
D000945:Antigens, Differentiation, T-Lymphocyte; D015699:HIV Envelope Protein gp120; D010446:Peptide Fragments; D015728:Receptors, HIV; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D012191:Retroviridae Proteins