Visualization of ANP receptor on glomeruli of bovine kidney by use of a specific antiserum.
Atrial natriuretic peptide (ANP) receptor was purified from the bovine lung by affinity chromatography and preparative gel electrophoresis. A specific antiserum was raised against the purified receptor and used to localize receptors immunohistochemically on bovine kidney sections. Intense staining was seen in glomeruli. This distribution corresponds to that of ANP binding sites revealed by previous autoradiographic analyses and matches a glomerular action of ANP suggested by physiological studies.
Shimonaka
M
M
Department of Chemistry, Tokyo Institute of Technology, Japan.
Saheki
T
T
Hagiwara
H
H
Hagiwara
Y
Y
Sono
H
H
Hirose
S
S
D000818:Animals; D002417:Cattle; D006651:Histocytochemistry; D007106:Immune Sera; D007678:Kidney Glomerulus / Q000032:analysis*; D008722:Methods; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000032:analysis*
D007106:Immune Sera; D011956:Receptors, Cell Surface; D017461:Receptors, Atrial Natriuretic Factor