title: Nontransmission of bluetongue virus by embryos from bluetongue virus-infected sheep.
abstract: Donor sheep were infected either by bites of bluetongue virus (BTV)-infected (serotype 11, "Texas Station strain") Culicoides variipennis or by inoculation with 100,000 median chicken embryo intravascular lethal doses of BTV (serotype 11) from a suspension made from infected C variipennis. Fourteen embryos from 4 BTV-infected ewes bred by rams not infected with BTV were transferred to 8 BTV-seronegative recipient ewes, and 35 embryos and 4 unfertilized eggs from 14 BTV-infected ewes bred by BTV-infected rams were transferred to 19 BTV-seronegative recipient ewes. Eleven pregnancies and 12 lambs resulted. None of the recipients or lambs seroconverted, and BTV was not isolated from the pregnant recipient ewes or their lambs at slaughter 30 days after parturition.
authors.affiliation: Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario.
authors.forename: W C
authors.initials: WC
authors.lastname: Hare
authors.forename: A J
authors.initials: AJ
authors.lastname: Luedke
authors.forename: F C
authors.initials: FC
authors.lastname: Thomas
authors.forename: R A
authors.initials: RA
authors.lastname: Bowen
authors.forename: E L
authors.initials: EL
authors.lastname: Singh
authors.forename: M D
authors.initials: MD
authors.lastname: Eaglesome
authors.forename: G C
authors.initials: GC
authors.lastname: Randall
authors.forename: A
authors.initials: A
authors.lastname: Bielanski
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000635:transmission*; D002519:Ceratopogonidae; D004624:Embryo Transfer; D005260:Female; D008297:Male; D011247:Pregnancy; D012756:Sheep