Characterization of beta-adrenergic receptors of human skeletal muscle obtained by needle biopsy.
Human skeletal muscle beta-adrenergic receptors were characterized by 125I-iodopindolol radioligand-binding studies of homogenates prepared from small muscle samples obtained by percutaneous needle biopsy from the gastrocnemius of six normal subjects. Binding was saturable, reversible, and stereospecific, with typical kinetics and a rank-order potency characteristic of a beta-adrenergic receptor. In saturation-binding studies, the receptor density was 9.7 +/- 1.9 fmol/mg protein, with a dissociation constant of 24 +/- 2.2 pM. Competition studies with selective antagonists revealed a population of receptors exclusively of the beta 2-subtype. Basal and isoproterenol-stimulated adenylate cyclase activities were 79 +/- 22 and 150 +/- 60 pmol adenosine 3',5'-cyclic monophosphate.min-1.mg protein-1, respectively. These results support pharmacological observations of beta-adrenergic receptor-mediated cellular responses in mammalian skeletal muscle. By use of these methods, small quantities of skeletal muscle obtained in this manner can be used to study in vivo beta-adrenergic receptor regulatory phenomena in humans.
Liggett
S B
SB
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Shah
S D
SD
Cryer
P E
PE
D000262:Adenylyl Cyclases / Q000032:analysis; D001667:Binding, Competitive; D001707:Biopsy, Needle; D006801:Humans; D007700:Kinetics; D008024:Ligands; D009132:Muscles / Q000032:analysis* / Q000201:enzymology; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000378:metabolism
D008024:Ligands; D011943:Receptors, Adrenergic, beta; D000262:Adenylyl Cyclases