title: Immunoregulatory abnormalities induced by experimental reovirus infection: functional alterations in T-cell subpopulations.
abstract: A primary anti-sheep red blood cell (SRBC) plaque assay system was used to analyze the effect of reovirus infection on immunoregulatory T-cells. A decrease in the plaque-forming cell (PFC) response of splenic lymphocytes was observed within 24 hr of infection with 10(9) or 10(11) particles of reovirus type 1 or reovirus type 3 and persisted for more than 7 days. In coculture experiments, T-cells from infected mice were found to produce less help to control B-cells and to suppress helper function mediated by control T-cells. This suppression did not require the presence of an Lyt1,2,3 cell. In addition, isolated Lyt1 cells from reovirus-inoculated mice provided less help than did Lyt1 cells from normal controls. These abnormalities were more marked following inoculation with reovirus type 3 than with type 1. Live virus was not required for these effects, as T-cells from mice inoculated with ultraviolet (uv)-inactivated reovirus when added to normal B-cells reproduced the effects of infection with live virus. Furthermore, these changes were not the result of alterations in the percentages or numbers of distinct Lyt-bearing T-cell subpopulations in the spleens of inoculated mice. Thus, humoral hyporesponsiveness following reovirus infection of adult mice is associated both with active T-cell suppression of B-cell help and with decreased help mediated by the Lyt1 subset.
authors.affiliation: Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: S L
authors.initials: SL
authors.lastname: Hauser
authors.forename: M
authors.initials: M
authors.lastname: Che
authors.forename: R J
authors.initials: RJ
authors.lastname: Fallis
authors.forename: H L
authors.initials: HL
authors.lastname: Weiner
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000473:pathology; D002469:Cell Separation; D007107:Immune System / Q000503:physiopathology*; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009000:Monocytes / Q000502:physiology; D012088:Reoviridae Infections / Q000473:pathology / Q000503:physiopathology*; D013154:Spleen / Q000473:pathology; D013601:T-Lymphocytes / Q000145:classification* / Q000502:physiology; D010948:Viral Plaque Assay