Ethanol alters microvasculature enzymes in the rat forebrain.
Forebrain arterioles were analyzed histochemically to determine the effects of an acute administration of ethanol on key enzymes of aerobic and anaerobic metabolism as well as on the hexose monophosphate shunt in rats. The enzymes were glucose 6-phosphate dehydrogenase, cytochrome oxidase, lactate dehydrogenase, beta-hydroxybutyrate dehydrogenase, and isocitrate dehydrogenase. All enzymes were quantified under two conditions: 1 h and 2 days after ethanol administration. Significant changes were noted in four of the five enzymes measured after 1 h and in all five enzymes when measured 2 days after ethanol administration. Our data suggest that ethanol may cause impaired metabolism in the forebrain microvasculature, which, in turn, may account for some of the characteristic behavioral effects of acute ethanol administration.
Trulson
M E
ME
Department of Anatomy, College of Medicine, Texas A&M University.
Ulissey
M J
MJ
D000818:Animals; D001160:Arterioles / Q000201:enzymology; D001921:Brain / Q000098:blood supply / Q000201:enzymology* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000032:analysis; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005751:Gastric Lavage; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006884:Hydroxybutyrate Dehydrogenase / Q000032:analysis; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D010427:Pentose Phosphate Pathway / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D000431:Ethanol; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D003576:Electron Transport Complex IV