Carbon tetrachloride toxicity on Escherichia coli exacerbated by superoxide.
The effects of carbon tetrachloride (CCl4) and paraquat on the growth of Escherichia coli were investigated. Paraquat at 10 mM caused some inhibition of growth of E. coli in trypticase-soy-yeast extract medium. CCl4 enhanced growth inhibition by paraquat in a concentration-dependent manner. In the absence of paraquat, CCl4 had no effect on growth rate or on surviving cell numbers at stationary phase. CCl4 did not prevent the induction of manganese-superoxide dismutase by paraquat. Under anaerobic conditions, CCl4 and paraquat exhibited no effect on E. coli. In the presence of Mn(II) and paraquat, intracellular superoxide dismutase was markedly induced and protected E. coli against the toxicity of CCl4 and paraquat. The reactive free radical CCl3OO-, which can be formed from the reaction of O2- with CCl4, may cause cell damage. The growth-inhibiting effects of polyhalides in the presence of paraquat followed the order CBrCl3 greater than CCl4 greater than CHCl3 greater than CH2Cl2, which is in accord with that of the reaction rates of these compounds with O2- and with their hepatotoxicities. These results suggest that O2- plays a role in the hepatotoxicity of polyhalides.
Yamamoto
H
H
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Nagano
T
T
Hirobe
M
M
D002251:Carbon Tetrachloride / Q000494:pharmacology*; D002374:Catalase / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D004347:Drug Interactions; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D005609:Free Radicals; D008345:Manganese / Q000494:pharmacology; D010269:Paraquat / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000096:biosynthesis; D013481:Superoxides / Q000494:pharmacology*
D005609:Free Radicals; D013481:Superoxides; D008345:Manganese; D002251:Carbon Tetrachloride; D002374:Catalase; D013482:Superoxide Dismutase; D010269:Paraquat