Rat transformation-associated proteins (TAP) induced by Moloney murine sarcoma virus interact with specific receptors on normal rat kidney cells.
In this report, data are presented to show that transformation-associated proteins (TAP) secreted from the transformed 6M2 cells have mitogenic activities in the stimulation of DNA synthesis and proliferation of normal rat kidney (NRK-2) cells and of nonpermissively grown 6M2 cells. TAP also bound specifically to NRK-2 cells with a binding dissociation constant (Kd) of 1.4 pM. Approximately 2 X 10(5) binding sites per cell were found. Therefore, TAP may represent a set of virally-induced growth stimulatory factors.
Li
W J
WJ
Steck
P
P
Bowen
J M
JM
Chan
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000503:physiopathology*; D006133:Growth Substances / Q000502:physiology*; D007328:Insulin / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D008934:Mitogens; D008970:Molecular Weight; D008980:Moloney murine sarcoma virus; D010455:Peptides / Q000502:physiology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015291:Transforming Growth Factors
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D007328:Insulin; D008934:Mitogens; D010455:Peptides; D011956:Receptors, Cell Surface; D015291:Transforming Growth Factors