Bacteriophage Mu sites required for transposition immunity.
Plasmids with bacteriophage Mu sequences receive additional Mu insertions 20-700 times less frequently than plasmids without Mu sequences. The Mu sites required for this transposition immunity were mapped near each end, either of which was sufficient. The left site was between 127 and 203 base pairs from the left end, and the right site was between 22 and 93 base pairs from the right end. These sequences include the innermost but not the outermost of the three binding sites for the Mu A transposition protein at each end of Mu. Transposition immunity was cis-acting and independent of its location on a target plasmid. An additional copy of an immunity site reduced transposition a factor of 10 further. Transposition immunity was seen both during full phage lytic growth, with all the bacteriophage Mu genes, and during normal cellular growth, with a mini-Mu element containing only the Mu c and ner regulatory and A and B transposition genes.
Darzins
A
A
Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
Kent
N E
NE
Buckwalter
M S
MS
Casadaban
M J
MJ
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data
D004251:DNA Transposable Elements