Lectin histochemistry in rat thyroid tumours.
The thyroid tumours and background goiterous and adenomatous lesions induced in rats with diisopropanolnitrosamine (DIPN) plus methylthiouracil (MTU), and regenerative thyroid tissues after wounding were studied by lectin histochemistry. Ten weeks after cessation of the carcinogen treatments, carcinomas invading the surrounding tissues and blood vessels (13/20) and papillary micronodules (11/20) were formed in the thyroid tissues. In general, the carcinoma lesion was solitary, and the papillary micronodules were multiple in a single thyroid gland. Among the lectins tested, Maclura pomifera (MPA) and Solanum tuberosum (STA) showed specific binding with both carcinoma and papillary micronodule lesions, but not with the background goiterous and adenomatous lesions and regenerative thyroid tissues. The former both lesions showed higher labelling indices with BUdR or 3H-thymidine and poorer thyroglobulin accumulation than the latters, thereby indicating their enhanced proliferative capability and depressed potency of cyto-differentiation. The common cytological and histochemical properties of carcinoma lesions and papillary micronodules allow us to regard the latter as pre-invading carcinoma lesions. The lectins MPA and STA may be, therefore, used as the specific markers of malignancy in rat thyroid carcinogenesis.
Imamura
Y
Y
Department of Pathology, Fukui Medical School, Japan.
Sugihara
H
H
Miyake
T
T
Ikeda
T
T
Noriki
S
S
Miyoshi
N
N
Nakanishi
K
K
Fukuda
M
M
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000096:biosynthesis; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D007399:Interphase; D037102:Lectins / Q000378:metabolism*; D008297:Male; D037121:Plant Lectins; D051381:Rats; D011919:Rats, Inbred Strains; D013954:Thyroglobulin / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology
D014408:Biomarkers, Tumor; D037102:Lectins; D037121:Plant Lectins; C041202:potato lectin; C034551:maclurin; D004247:DNA; D013954:Thyroglobulin; D006735:Horseradish Peroxidase