title: Anion exchange chromatography of 99mTc(Sn)-MDP complexes: influence of eluent composition, determination of void volume and charge of the main component.
abstract: 99mTc(Sn)-MDP complexes have been prepared by reduction of 99mTcO4- by Sn(II) in the presence of MDP. These complexes were separated on an anion exchange column. The necessity of the addition of the ligand and the reducing agent to the eluent to avoid decomposition during chromatography is demonstrated. For the main component that is found at pH 5 the ionic charge was calculated according to the method of Wilson and Pinkerton [Anal. Chem. 57,246 (1985)] and the method of Russell and Bishoff [Int. J. Appl. Radiat. Isot. 35,859 (1985)]. With the first method a charge of -4.2 +/- 0.3 was obtained, with the second a charge of -5.1 +/- 0.6. An accurate method to determine the void volume of the ion exchange column is described.
authors.affiliation: Analytical Chemistry Laboratory, State University of Utrecht, The Netherlands.
authors.forename: Y M
authors.initials: YM
authors.lastname: Huigen
authors.forename: T G
authors.initials: TG
authors.lastname: Tji
authors.forename: W J
authors.initials: WJ
authors.lastname: Gelsema
authors.forename: C L
authors.initials: CL
authors.lastname: de Ligny
mesh_terms: D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008968:Molecular Conformation; D013669:Technetium Tc 99m Medronate / Q000138:chemical synthesis
chemical_list: D007202:Indicators and Reagents; C023568:technetium Tc 99m (Sn)methylenediphosphonate; D013669:Technetium Tc 99m Medronate