Autoradiographic demonstration of gastrin-releasing peptide-binding sites in the rat gastric mucosa.
The location of [125I]iodotyrosyl gastrin-releasing peptide-binding sites in the rat fundic mucosa was studied. Peptide specificity was demonstrated by competitive binding studies using the addition of a large amount of cold gastrin-releasing peptide or substance P. Autoradiography of the stomach tissue was carried out by freeze-drying, embedding in Epon, wet-sectioning with ethylene glycol, and dry-mounting the emulsion film by the wire-loop method to prevent loss of the labeled substance. Specific binding sites of gastrin-releasing peptide were found on D cells, surface mucus cells, and parietal cells, whereas few binding sites were seen on the chief or mucus neck cells.
Nakamura
M
M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Oda
M
M
Kaneko
K
K
Akaiwa
Y
Y
Tsukada
N
N
Komatsu
H
H
Tsuchiya
M
M
D000818:Animals; D001345:Autoradiography; D001839:Bombesin / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D019886:Gastrin-Releasing Peptide; D005755:Gastrins / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D010295:Parietal Cells, Gastric / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter / Q000378:metabolism*
D005755:Gastrins; D007457:Iodine Radioisotopes; D010455:Peptides; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter; D019886:Gastrin-Releasing Peptide; D001839:Bombesin