Failure of leukotriene B4 to translocate calcium across phosphatidylcholine membranes.
It has been reported that leukotriene B4 can translocate calcium across model membranes (Serhan et. al., (1982) J. Biol. Chem., 257: 4746). Such ionophoretic behavior could account for its biological effects. We have examined the effect of chromatographically pure leukotriene B4 on Ca2+ permeability when added exogenously at 3 microM to phosphatidylcholine liposomes and when incorporated at 5 mole % in the lipid mixture used to prepare liposomes. No effect was observed with either procedure. An oxidized preparation of leukotriene B4 stimulated calcium permeability, however, suggesting that oxidation may account for the previously reported ionophoretic behavior of leukotriene B4.
Holmes
R P
RP
Urology Section, Bowman Gray School of Medicine, Winston-Salem, NC 27103.
Yoss
N L
NL
Marshall
L A
LA
D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D007975:Leukotriene B4 / Q000494:pharmacology*; D008081:Liposomes; D008567:Membranes, Artificial; D010539:Permeability; D010713:Phosphatidylcholines
D008081:Liposomes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D007975:Leukotriene B4; D002118:Calcium