The coenrichment of stem cells, prothymocytes, and stromal elements with ecotropic retrovirus-producing cells from the bone marrow of leukemia-prone AKR mice.
Ecotropic virus-producing cells in the bone marrow of the leukemia-prone AKR strain of mice were significantly enriched by fractionation on discontinuous density gradients of Percoll and were found in a low-density population of cells comprised predominantly of medium to large blast cells. The high ecotropic virus-producing low-density bone marrow cell population was also found to be significantly enriched in pluripotent stem cells, prothymocytes, and stromal elements. During the period of time defined by a window for successful leukemosuppressive immunotherapy of AKR mice, virus-producing cells were exclusively detected in this fraction of bone marrow cells, implicating the functional classes of cells coenriched in this fraction as both potential targets of anti-viral immunotherapy and responsible for the seeding of the spleen and thymus with infectious ecotropic virus.
Buckheit
R W
RW
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
Kurtzberg
J
J
Bolognesi
D P
DP
Weinhold
K J
KJ
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000382:microbiology; D001854:Bone Marrow Cells; D002469:Cell Separation; D009052:Leukemia Virus, Murine / Q000254:growth & development*; D051379:Mice; D008806:Mice, Inbred AKR / Q000033:anatomy & histology / Q000382:microbiology*; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000382:microbiology; D013950:Thymus Gland / Q000166:cytology* / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis
D000954:Antigens, Surface; D014764:Viral Proteins