Effect of vitamin D status on chick kidney proteins: detection of a 45-kilodalton mitochondrial protein suppressed by vitamin D.
Two-dimensional polyacrylamide gel electrophoresis along with L-[35S]methionine radiolabeling studies were used to examine the effect of chronic vitamin D status on the composition and relative abundance of chick kidney proteins. Comparison of silver-stained gels revealed no extensive differences in either the electrophoretic mobility or the amounts of kidney proteins present in the mitochondrial fraction from vitamin D-replete and vitamin D-deficient chicks. A similar result was obtained in studies with L-[35S]methionine-labeled proteins. Vitamin D deficiency specifically elevated levels of a 45-kilodalton mitochondrial protein (pI 5.0 to 5.5) by approximately 5- to 12-fold relative to amounts present in vitamin D-replete tissue. This protein could not be detected in postmitochondrial supernatant fractions and was only faintly visible in crude kidney homogenates. The specificity of the observed suppression of this 45-kilodalton protein by vitamin D suggests that it may play an important role in renal functions influenced by the vitamin D endocrine system.
Kain
S R
SR
Department of Biochemistry, University of California, Riverside 92521.
Kamrath
K S
KS
Henry
H L
HL
D000818:Animals; D002645:Chickens; D002762:Cholecalciferol / Q000494:pharmacology; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008715:Methionine / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D012016:Reference Values; D013462:Sulfur Radioisotopes; D014808:Vitamin D Deficiency / Q000378:metabolism*
D011506:Proteins; D013462:Sulfur Radioisotopes; D002762:Cholecalciferol; D008715:Methionine