Complete nucleotide sequence and characterization of the 5'-flanking region of mammalian elongation factor 2 gene.
The hamster elongation factor 2 gene was isolated from genomic libraries of diphtheria toxin- and Pseudomonas aeruginosa exotoxin A-resistant cells containing non-ADP-ribosylatable elongation factor 2, and its structure was determined by a combination of restriction endonuclease mapping and DNA sequence analysis. The entire gene is about 6 kilobases long and has 13 exons. Almost all the introns are about 90-200 bases long, except the first and third, which are about 1 kilobase and 400 bases long, respectively. The first exon is processed just after the initiation codon for translation. The promoter of this gene was also characterized. As this gene contains the mutation conferring resistance to diphtheria toxin and P. aeruginosa exotoxin A, introduction of this gene into mammalian cells results in expression of toxin resistance. Using this characteristic, gene expression by deletion mutants of the 5'-flanking region were examined, and results showed that about 60 base pairs upstream of the TATA sequence were most efficient for expression of the elongation factor 2 gene.
Nakanishi
T
T
Department of Cell Biology, National Institute for Basic Biology, Aichi, Japan.
Kohno
K
K
Ishiura
M
M
Ohashi
H
H
Uchida
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D006224:Cricetinae; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005091:Exons; D007438:Introns; D008969:Molecular Sequence Data; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000235:genetics*; D014176:Protein Biosynthesis
D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D004262:DNA Restriction Enzymes