[Enzyme activity of gluconeogenesis in dietary thiamine deficiency].
Activity of the main enzymes of gluconeogenesis under food thiamine deficiency was studied in tissues of satiated and 48-hour starved rats. Starvation of control rats (with no vitamin B1-deficiency) led to increased activity of glucose 6-phosphatase (G-6-P) in the liver, kidney and small intestinal mucosa, and of phosphoenolpyruvate carboxykinase (PEPCK) in the liver and kidneys. Fructose 1,6-diphosphatase (F-D-P) activity in the control animals was not changed in the liver and kidneys but decreased in the small intestinal mucosa. Starvation of the test animals (with vitamin B1-deficiency) was attended by increased G-6-P and PEPCK activity in the liver and kidneys, and F-D-P activity in the liver. Thiamine deficiency led to lowered G-6-P and F-D-P activities in the liver and kidneys and PEPCK in the liver of the test animals as compared to the control. The data obtained have evidenced disorders in the gluconeogenesis under conditions of vitamin B1-deficiency.
Maslovskaia
A A
AA
Klimovich
V V
VV
Lukashik
N K
NK
D000818:Animals; D005215:Fasting; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005943:Gluconeogenesis; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D008297:Male; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D051381:Rats; D013832:Thiamine Deficiency / Q000201:enzymology*; D013997:Time Factors; D014018:Tissue Distribution; D014174:Transketolase / Q000097:blood
D005954:Glucosephosphate Dehydrogenase; D014174:Transketolase; D006597:Fructose-Bisphosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)
Aktivnost' fermentov gliukoneogeneza pri pishchevoĭ nedostatochnosti tiamina.