title: The production of antibodies which recognize opiate receptors on murine leukocytes.
abstract: An antibody has been developed which recognizes opiate receptors on cells of the immune system. This antibody blocks specific binding of the radiolabeled opiate receptor ligand, 3H-dihydromorphine, to receptors on murine splenocytes. Additionally, the anti-receptor antibody competes with beta-endorphin, met-enkephalin, and naloxone for the same binding site on the leukocytes. Moreover, the anti-receptor antibody possesses agonist activity similar to beta-endorphin in suppressing cAMP production by lymphocytes. These results suggest the development of an antibody which recognizes classical opiate receptors on cells of the immune system.
authors.affiliation: Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
authors.forename: D J
authors.initials: DJ
authors.lastname: Carr
authors.forename: K L
authors.initials: KL
authors.lastname: Bost
authors.forename: J E
authors.initials: JE
authors.lastname: Blalock
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D000242:Cyclic AMP / Q000096:biosynthesis; D004723:Endorphins / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D010455:Peptides / Q000276:immunology; D011957:Receptors, Opioid / Q000276:immunology* / Q000502:physiology; D013154:Spleen / Q000166:cytology
chemical_list: D000906:Antibodies; D004723:Endorphins; D010455:Peptides; D011957:Receptors, Opioid; D000242:Cyclic AMP