Expression of hepatitis A virus capsid sequences in insect cells.
A cDNA coding for hepatitis A virus (HAV) VP1 and portions of the flanking VP3 and P2 sequences was inserted into the genome of Autographa californica nuclear polyhedrosis virus under the control of the polyhedrin promoter and translational start codon. Cells infected with recombinant virus produced high levels of a 55 kDa protein, identified as containing HAV VP1 by reactivity with anti-VP1 serum. The expressed protein also reacted on immunoblots with human HAV convalescent sera as well as sera from rabbits immunized with intact HAV. This protein was found predominantly in the cytoplasm of infected insect cells, probably as an insoluble aggregate.
Harmon
S A
SA
Department of Cellular, Viral and Molecular Biology, University of Utah, Salt Lake City.
Johnston
J M
JM
Ziegelhoffer
T
T
Richards
O C
OC
Summers
D F
DF
Ehrenfeld
E
E
D000818:Animals; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D002999:Clone Cells; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006507:Hepatovirus / Q000235:genetics*; D006801:Humans; D007118:Immunoassay; D007304:Insect Viruses / Q000235:genetics*; D009036:Moths; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011817:Rabbits
D004262:DNA Restriction Enzymes