Generation of human myogenic cell lines by the transformation of primary culture with origin-defective SV40 DNA.
The gene transfection technique was applied to establish clonal human skeletal muscle cell lines. DNA of a replication origin-defective mutant of SV40 was transfected into a primary culture of human skeletal muscle by the DNA-calcium phosphate co-precipitation method, and myoblast-derived cells were selected from among the transformed cells and cloned. The myogenic clonal cells exhibited an enhanced growth rate and an unlimited life span, which indicated that a stable supply of a large quantity of cultured human myogenic cells without contaminating fibroblasts was possible. In addition, despite the transformation, the transformed clones retained a certain differentiation ability, that is, they could form multinucleated cells or express a muscle-specific isomer of creatine kinase. These characteristics of transformed myogenic cells should be of great value in studies on the molecular pathologies of various myopathies.
Nakamigawa
T
T
Department of Pediatrics, Jichi Medical School, Tochigi, Japan.
Momoi
M Y
MY
Momoi
T
T
Yanagisawa
M
M
D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D004279:DNA, Viral; D006801:Humans; D007223:Infant; D008297:Male; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000378:metabolism
D004279:DNA, Viral; D003402:Creatine Kinase