title: [M-cholinergic regulation of Na+,K+-ATPase activity of vesicular preparations of sarcolemma from the myocardium and intestinal smooth muscles].
abstract: Acetylcholine (10(-7)-10(-2) M) enhanced the Na+, K+-ATPase activity in sarcolemmal vesicles from myocardium and intestinal smooth muscle. The stimulation of the enzyme from canine ventricles reaches 150% and was less pronounced (10-20%) in the case of frog myocardium and canine ileal muscles. The activating action of the neurotransmitter was simulated by gramicidin D (1-5 microM), but not by valinomycin 1-5 microM), blocked both by ouabain (200 microM) and atropine (0.1 microM), a muscarinic cholinergic antagonist. The activating action disappeared after treatment of membranes with alamethicin, a pore-producing antibiotic (0.8 mg/mg of protein). It is suggested that an increase in the Na+, K+-ATPase activity caused by acetylcholine is induced by Na+ which permeate the sarcolemmal vesicles through the ionic channel coupled with muscarinic acetylcholine receptor.
authors.forename: M P
authors.initials: MP
authors.lastname: Danilenko
authors.forename: Z Sh
authors.initials: ZSh
authors.lastname: Smagulova
authors.forename: V K
authors.initials: VK
authors.lastname: Turmukhambetova
authors.forename: R D
authors.initials: RD
authors.lastname: Omarova
authors.forename: O V
authors.initials: OV
authors.lastname: Esyrev
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001001:Anura; D004285:Dogs; D004789:Enzyme Activation; D007082:Ileum / Q000201:enzymology; D009130:Muscle, Smooth / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D012508:Sarcolemma / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D011976:Receptors, Muscarinic; D000254:Sodium-Potassium-Exchanging ATPase; D000109:Acetylcholine
vernacular_title: M-kholinergicheskaia reguliatsiia aktivnosti Na+,K+-ATP-azy v vezikuliarnykh preparatakh sarkolemmy miokarda i gladkikh myshts kishechnika.