title: Adenovirus type-2 in a patient with lethal hemorrhagic colonic ulcers and chronic active Epstein-Barr virus infection.
abstract: An adenovirus type-2 was isolated from peripheral blood lymphocytes and throat washings from a patient with severe chronic active Epstein-Barr virus infection. Despite the Epstein-Barr virus reactivation, attempts to establish spontaneous lymphoblastoid cell lines from peripheral blood lymphocytes and to immortalize cord lymphocytes with throat washings were unsuccessful due to a marked cytopathic effect. The supernatants from the cultures induced cytopathic effect in cultured cord lymphocytes, MRC-5 cells, A-549 cells, or Vero cells. Virus particles with adenovirus morphology were seen by electron microscopy. Using type-specific antisera, the isolate was identified as adenovirus type-2. In addition, both Epstein-Barr virus and adenovirus type-2 genomes were seen in the colonic tissues and spleen. These results suggest that the combination of Epstein-Barr virus and adenovirus type-2 may be etiologic agents in the development of chronic active Epstein-Barr virus infection in this patient.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha.
authors.forename: M
authors.initials: M
authors.lastname: Okano
authors.forename: G M
authors.initials: GM
authors.lastname: Thiele
authors.forename: J R
authors.initials: JR
authors.lastname: Davis
authors.forename: W M
authors.initials: WM
authors.lastname: Nauseef
authors.forename: F
authors.initials: F
authors.lastname: Mitros
authors.forename: D T
authors.initials: DT
authors.lastname: Purtilo
mesh_terms: D000257:Adenoviridae Infections / Q000150:complications*; D000258:Adenovirus Infections, Human / Q000150:complications*; D000260:Adenoviruses, Human / Q000302:isolation & purification; D000328:Adult; D002908:Chronic Disease; D003108:Colonic Diseases / Q000382:microbiology*; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D006471:Gastrointestinal Hemorrhage / Q000382:microbiology*; D006566:Herpesviridae Infections / Q000150:complications*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology; D008297:Male; D010614:Pharynx / Q000382:microbiology; D014456:Ulcer / Q000382:microbiology; D014771:Virion / Q000648:ultrastructure