title: Isolation of the yeast INO4 gene, a positive regulator of phospholipid biosynthesis.
abstract: Yeast ino4 mutants are auxotrophic for the phospholipid precursor inositol and have pleiotropic defects in phospholipid synthesis. The mutants are unable to derepress the cytoplasmic enzyme, inositol-1-phosphate synthase and they exhibit reduced synthesis of methylated phospholipids, particularly phosphatidyl-choline. The INO4 gene is believed to encode a positive regulator involved in coordinate control of phospholipid synthesis. In the present study, we report the isolation of two clones containing the INO4 gene. The clones share a region of homology and were mapped to the INO4 locus. Southern blot analysis revealed that the cloned DNA contained both unique and repetitive DNA.
authors.affiliation: Department of Genetics and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
authors.forename: L S
authors.initials: LS
authors.lastname: Klig
authors.forename: D K
authors.initials: DK
authors.lastname: Hoshizaki
authors.forename: S A
authors.initials: SA
authors.lastname: Henry
mesh_terms: D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D005800:Genes, Fungal; D005809:Genes, Regulator; D009154:Mutation; D009710:Nucleotide Mapping; D010743:Phospholipids / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D010743:Phospholipids; D004262:DNA Restriction Enzymes