Interleukin 1 alpha acts as an autocrine growth factor for RPMI 1788, an Epstein-Barr virus-transformed human B cell line.
The Epstein-Barr virus-transformed B cell line RPMI 1788 constitutively produces autocrine growth factors with molecular masses of 17 kDa, 24 kDa and 35 kDa. All three molecular forms were completely neutralized with anti-interleukin (IL) 1 alpha antiserum. Although IL 1 alpha and IL 1 beta mRNA were both equally detectable by Northern blotting, no IL 1 beta activity was found in partially purified RPMI 1788 supernatant. The growth of low density-seeded RPMI 1788 cells is specifically dependent on the presence of either IL 1 alpha or IL 1 beta. Since no other cytokine was found to be capable of sustaining proliferation, this cell line is suitable for the identification and quantification of IL 1, even in the presence of other cytokines.
Vandenabeele
P
P
Laboratory of Molecular Biology, State University of Gent, Belgium.
Jayaram
B
B
Devos
R
R
Shaw
A
A
Fiers
W
W
D001402:B-Lymphocytes / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002472:Cell Transformation, Viral; D006133:Growth Substances; D004854:Herpesvirus 4, Human; D006801:Humans; D007158:Immunologic Techniques; D007375:Interleukin-1 / Q000502:physiology*; D008970:Molecular Weight; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
D006133:Growth Substances; D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins