Cotransposition of a highly repetitive DNA element with flanking sequences in the genome of the midge Chironomus thummi.
A family of highly repetitive DNA elements, the Cla-elements, is present in the genomes of the two sibling species Chironomus th. thummi and Ch. th. piger. These Cla-elements are organized in large tandem repetitive clusters as well as occurring as interspersed monomeric elements, in both subspecies. The analysis of a monomeric Cla-element and several kilobases of its flanking sequences from Ch. th. piger revealed that the short Cla-elements are cotransposed together with adjacent DNA. We found the same association of Cla-elements with specific flanking DNA in clones obtained from the rDNA of Ch. th. thummi and from nonribosomal Cla-DNA of Ch. th. piger. The Cla-element-flanking DNA is clearly also repetitive, but mainly of interspersed organization.
Hankeln
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Institute of Genetics, Ruhr-Universität, Bochum, West Germany.
Schmidt
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ER
D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004175:Diptera / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements; D004247:DNA; D004262:DNA Restriction Enzymes