SGHTL-34, a thyrotrophin-responsive immortalised human thyroid cell line generated by transfection.
Normal human thyrocytes were immortalised by electroporation-mediated transfection with the plasmid pSV3neo. One resultant cell line (SGHTL-34) contains approximately 10 copies of simian virus 40 (SV40) early region incorporated into the genome and has been in continuous monolayer culture for 9 months. SGHTL-34 cells grow rapidly (doubling time 30 h) and contain cytokeratin filaments. They demonstrate a morphological change in response to thyrotrophin (TSH) and possess a TSH- and forskolin-sensitive adenylate cyclase, the thresholds for stimulation being 10 microU/ml bovine TSH- and forskolin-sensitive development of this novel human thyrocyte cell line may allow further study of the regulation of human thyroid growth and differentiation.
Whitley
G S
GS
Department of Immunology, St. George's Hospital Medical School, London, U.K.
Nussey
S S
SS
Johnstone
A P
AP
D000262:Adenylyl Cyclases / Q000378:metabolism; D002460:Cell Line; D002472:Cell Transformation, Viral; D006801:Humans; D010957:Plasmids; D013539:Simian virus 40; D013961:Thyroid Gland / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D013972:Thyrotropin / Q000494:pharmacology*; D014162:Transfection
D013972:Thyrotropin; D000262:Adenylyl Cyclases