Meiotic expression of human ornithine transcarbamylase in the testes of transgenic mice.
In an attempt to use mouse metallothionein-I (mMT-I) regulatory sequences to direct expression of human ornithine transcarbamylase in the liver of transgenic animals, fusion genes joining either 1.6 kilobases or 185 base pairs of the mMT-I regulatory region to the human ornithine transcarbamylase protein-coding sequence were used to produce transgenic mice. In mice carrying the fusion gene with 1.6 kilobases of the mMT-I 5'-flanking sequences, transgene expression was observed in a wide range of tissues, but, unexpectedly, expression in liver was never observed. Surprisingly, in mice carrying the fusion gene regulated by only 185 base pairs of the mMT-I 5'-flanking sequences, the transgene was expressed exclusively in male germ cells during the tetraploid, pachytene stage of meiosis.
Kelley
K A
KA
Department of Human Genetics, Yale University School of Medicine, New Haven, Connecticut.
Chamberlain
J W
JW
Nolan
J A
JA
Horwich
A L
AL
Kalousek
F
F
Eisenstadt
J
J
Herrup
K
K
Rosenberg
L E
LE
D000818:Animals; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D008099:Liver / Q000201:enzymology; D008297:Male; D008540:Meiosis; D051379:Mice; D008822:Mice, Transgenic; D009693:Nucleic Acid Hybridization; D009954:Ornithine Carbamoyltransferase / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D013737:Testis / Q000166:cytology / Q000201:enzymology*
D009954:Ornithine Carbamoyltransferase; D004262:DNA Restriction Enzymes