Identification of the coronavirus MHV-JHM mRNA 4 product.
A bacterial expression vector was constructed to encode a fusion protein which had, at its carboxy terminus, a polypeptide encoded within the 5' proximal open reading frame of the coronavirus MHV-JHM mRNA 4. This polypeptide was isolated and used to produce an antiserum. The antiserum reacted specifically with a 15,000 Mr polypeptide synthesized in MHV-JHM-infected cells, or in vitro translations of infected cell poly(A) RNA enriched for mRNA 4. These results demonstrate the translational activity of mRNA 4 during infection, identify conclusively the translation product and provide a means to investigate the synthesis and function of this protein.
Ebner
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Institute of Virology, University of Würzburg, F.R.G.
Raabe
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Siddell
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D000914:Antibodies, Viral / Q000276:immunology; D005814:Genes, Viral; D006517:Murine hepatitis virus / Q000235:genetics* / Q000276:immunology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins