The fate of instilled pulmonary surfactant in normal and quartz-treated rats.
Naturally prepared radiolabelled pulmonary surfactant can be rapidly cleared from the alveolar surface to the lung tissue after intratracheal instillation into experimental rats. This clearance is both time- and dose-dependent, a large dose (10 mg/animal) becoming associated with lung tissue more rapidly than a smaller more physiological dose (0.75 mg/animal). The data indicate that extracellular dipalmitoyl-phosphatidylcholine, the major component of pulmonary surfactant, is not catabolized at the alveolar surface. Alveolar free cells (mainly macrophages) appear to play a minor role in surfactant clearance. Quartz-induced phospholipidosis does not lead to an alteration in the rate of bulk surfactant clearance from the alveolar surface, although the initial distribution of the removed phospholipid complex may change in relation to the enlarged heterogenous free cell population.
Lewis
R W
RW
Department of Biochemistry, University College, Cardiff, Wales, U.K.
Harwood
J L
JL
Richards
R J
RJ
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011663:Pulmonary Surfactants / Q000378:metabolism*; D011791:Quartz / Q000494:pharmacology*; D051381:Rats; D012822:Silicon Dioxide / Q000494:pharmacology*; D014018:Tissue Distribution
D011663:Pulmonary Surfactants; D011791:Quartz; D015060:1,2-Dipalmitoylphosphatidylcholine; D012822:Silicon Dioxide