Molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the L protein.
The sequence of the gene encoding the L protein of the human parainfluenza 3 virus was determined by direct dideoxy sequence analysis of the genomic 50 S RNA and confirmed by molecular cloning and sequence analysis of recombinant clones. A series of three overlapping clones was generated by primer extension using genomic 50 S RNA as the template. These clones originate within the 5' end of the hemagglutinin-neuraminidase gene, span the entire L gene, and extend into the extracistronic 5' end of the viral RNA. The L gene extends 6755 nucleotides (inclusive of the putative transcription initiation and polyadenylation signal sequences) and encodes a protein consisting of 2233 amino acids (MW 255,812). There are 44 nucleotides downstream of the putative polyadenylation signal sequence which may represent a negative-strand leader. The complementary sequence of the extracistronic region is nearly identical to the 3' end of the viral RNA. Thirty-three of the first thirty-nine nucleotides of the 3' ends of the plus and minus strands are conserved. Comparison of amino acid sequence homology with other paramyxoviral L proteins indicates a high degree of sequence conservation with Sendai virus (62%) and Newcastle disease virus (28%). In addition, four smaller regions were identified which shared extensive homology with the L protein of vesicular stomatitis virus, a member of the Rhabdoviridae family.
Galinski
M S
MS
Division of Molecular Virology, James N. Gamble Institute of Medical Research, Cincinnati, Ohio 45219.
Mink
M A
MA
Pons
M W
MW
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010224:Parainfluenza Virus 3, Human / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D018108:Respirovirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D000596:Amino Acids; D012367:RNA, Viral; D014764:Viral Proteins; C056831:parainfluenza 3 virus, L Protein; D004247:DNA; D012321:DNA-Directed RNA Polymerases