Effects of chronic allopurinol therapy on purine metabolism in Duchenne muscular dystrophy.
Adenine, adenosine, inosine, hypoxanthine, xanthosine, xanthine, guanine and guanosine blood levels in 11 Duchenne muscular dystrophy patients treated with allopurinol, 10 untreated patients and 8 healthy controls, were determined by HPLC. Serum ADA, PNP and 5'-NT were also determined. Untreated patients showed lower adenine (p less than 0.001) and higher adenosine, xanthine, ADA and PNP levels (p less than 0.01) than controls. Treated patients had lower adenine and higher xanthine levels (p less than 0.001), but higher hypoxanthine, xanthosine and guanine levels (p less than 0.001), than controls, with normal ADA and PNP. The changes observed in ADA and PNP levels suggest an involvement of these enzymes in accelerated degradation of purines in Duchenne dystrophy.
Castro-Gago
M
M
Lojo
S
S
Novo
I
I
del Rio
R
R
Peña
J
J
Rodriguez-Segade
S
S
D015720:5'-Nucleotidase; D000243:Adenosine Deaminase / Q000097:blood; D000493:Allopurinol / Q000494:pharmacology / Q000627:therapeutic use*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D009136:Muscular Dystrophies / Q000188:drug therapy* / Q000378:metabolism; D009708:Nucleotidases / Q000097:blood; D011683:Purine-Nucleoside Phosphorylase / Q000097:blood; D011687:Purines / Q000097:blood*
D011687:Purines; D000493:Allopurinol; D011683:Purine-Nucleoside Phosphorylase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase