title: Rapid, sensitive detection of plasma IL-1 by extraction and bioassay.
abstract: A method for separating IL-1 from plasma inhibitors by silica extraction has been developed and coupled to a highly sensitive bioassay using the LBRM TG6 cell line and the I1-2 dependent HT2A cell line. Using this assay we have detected IL-1 activity in plasma from patients undergoing elective surgery.
authors.affiliation: Department of Biochemistry, Royal Infirmary, Glasgow, U.K.
authors.forename: J J
authors.initials: JJ
authors.lastname: Series
authors.forename: D
authors.initials: D
authors.lastname: Shapiro
authors.forename: I
authors.initials: I
authors.lastname: Cameron
authors.forename: J
authors.initials: J
authors.lastname: Smith
authors.forename: W D
authors.initials: WD
authors.lastname: Fraser
authors.forename: O J
authors.initials: OJ
authors.lastname: Garden
authors.forename: A
authors.initials: A
authors.lastname: Shenkin
mesh_terms: D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D006801:Humans; D007375:Interleukin-1 / Q000097:blood* / Q000302:isolation & purification; D008223:Lymphoma / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*; D012822:Silicon Dioxide; D013601:T-Lymphocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D007375:Interleukin-1; D012822:Silicon Dioxide