title: A recombinant vaccinia virus expressing herpes simplex virus type 1 glycoprotein B induces cytotoxic T lymphocytes in mice.
abstract: Spleen cells from BALB/c (H-2d) mice vaccinated with vgB11, a recombinant vaccinia virus which expresses glycoprotein B (gB) of herpes simplex virus type 1 (HSV-1), lysed EMT6 (H-2d) target cells infected with vgB11 or with HSV-1 but did not lyse uninfected EMT6 cells or infected L-929 (H-2k) target cells. Unlabelled target cell competition of lysis showed that only syngeneic cells infected with vgB11 or HSV-1 inhibited lysis of radiolabelled HSV-1-infected targets. These results demonstrate that vgB11 induces H-2-restricted anti-HSV-1 cytotoxic T lymphocytes and that gB is the target antigen.
authors.affiliation: Department of Molecular Biochemistry, City of Hope National Medical Center, Duarte, California 91010.
authors.forename: E
authors.initials: E
authors.lastname: McLaughlin-Taylor
authors.forename: D E
authors.initials: DE
authors.lastname: Willey
authors.forename: E M
authors.initials: EM
authors.lastname: Cantin
authors.forename: R
authors.initials: R
authors.lastname: Eberle
authors.forename: B
authors.initials: B
authors.lastname: Moss
authors.forename: H
authors.initials: H
authors.lastname: Openshaw
mesh_terms: D000818:Animals; D006183:H-2 Antigens / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D006183:H-2 Antigens; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus