title: DNA bending is induced in an enhancer by the DNA-binding domain of the bovine papillomavirus E2 protein.
abstract: The E2 gene of bovine papillomavirus type 1 has been shown to encode a DNA-binding protein and to trans-activate the viral enhancer. We have localized the DNA-binding domain of the E2 protein to the carboxyl-terminal 126 amino acids of the E2 open reading frame. The DNA-binding domain has been expressed in Escherichia coli and partially purified. Gel retardation and DNase I "footprinting" on the bovine papillomavirus type 1 enhancer identify the sequence motif ACCN6GGT (in which N = any nucleotide) as the E2 binding site. Using electrophoretic methods we have shown that the DNA-binding domain changes conformation of the enhancer by inducing significant DNA bending.
authors.affiliation: Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.
authors.forename: C
authors.initials: C
authors.lastname: Moskaluk
authors.forename: D
authors.initials: D
authors.lastname: Bastia
mesh_terms: D001483:Base Sequence; D052688:Bovine papillomavirus 1; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; D014764:Viral Proteins; D003850:Deoxyribonuclease I