Atrial natriuretic polypeptides elevate the level of cyclic GMP in the rat choroid plexus.
We studied the effect of synthetic rat alpha-atrial natriuretic polypeptide (1-28) (ANF-(99-126)) and related atrial natriuretic polypeptides on the accumulation of cyclic GMP (cGMP) in isolated rat choroid plexus in vitro, the objective being to determine whether ANF-(99-126) binding sites are physiologically active receptors. One microM of ANF-(99-126) increased the level of cGMP within 30 s and the accumulation reached the maximum level 5 min after adding of ANF-(99-126) to the incubation media. Significant increase in the level of cGMP depended on concentrations of the ANF-(99-126), ANF-(101-126) and ANF-(103-125), with a range of 10.0 nM to 10.0 microM. These data indicate that binding sites for ANF-(99-126) in the rat choroid plexus may be physiologically active receptors, possibly linked to the production of cerebrospinal fluid.
Tsutsumi
K
K
Department of Neurosurgery, Nagasaki University School of Medicine, Japan.
Niwa
M
M
Kawano
T
T
Ibaragi
M
M
Ozaki
M
M
Mori
K
K
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002831:Choroid Plexus / Q000187:drug effects / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP