A non-denaturing gel electrophoresis system for the purification of membrane bound proteins.
A new method is described for the purification of a membrane bound glycoprotein, the kappa opioid receptor from human placental tissue. The method uses preparative slab-gel electrophoresis in the presence of the non-denaturing detergent CHAPS. A linear relationship between log molecular weight and SDS PAGE electrophoretic mobility of known molecular weight markers, in the presence of CHAPS, is observed. Using this method, we were able partially to purify an 3H-etorphine binding glycoprotein, from placental villus tissue, with an apparent molecular weight range of 60-70,000. The iodinated glycoprotein migrates in SDS PAGE with an apparent molecular weight of 63,000. This method may be useful for the isolation of membrane bound proteins, especially when an affinity ligand is not available.
Cavinato
A G
AG
Memphis State University, TN 38152.
Macleod
R M
RM
Ahmed
M S
MS
D002793:Cholic Acids; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins / Q000302:isolation & purification*; D008970:Molecular Weight; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D011957:Receptors, Opioid / Q000032:analysis
D002793:Cholic Acids; D003902:Detergents; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D011957:Receptors, Opioid; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate