Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5.
Conditioned medium from the Con A-treated mouse helper T-cell clone Ly1+2-/9 contains activities that enhance the production of IgA by mouse B cells and induce human cord blood cells to form eosinophil colonies. We have isolated a cDNA sequence that expresses IgA-enhancing factor and eosinophil colony-stimulating factor activities from a cDNA library prepared from activated Ly1+2-/9 cells. Based on homology with the mouse cDNA sequence, a human cDNA sequence coding for an interleukin with IgA-enhancing factor and eosinophil colony-stimulating factor activities was isolated from a cDNA library prepared from a human T-cell clone stimulated with anti-T3 antibody and phorbol 12-myristate 13-acetate. DNA sequence analyses revealed that mouse and human cDNA clones encode proteins of 133 and 134 amino acids, respectively, that are identical to cDNA clones encoding the T-cell replacing factor I and B-cell growth factor II activities. These results establish that a single cDNA clone encodes a protein that acts as a growth and differentiation factor for both B cells and eosinophils.
Yokota
T
T
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94034-1104.
Coffman
R L
RL
Hagiwara
H
H
Rennick
D M
DM
Takebe
Y
Y
Yokota
K
K
Gemmell
L
L
Shrader
B
B
Yang
G
G
Meyerson
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D003001:Cloning, Molecular; D003115:Colony-Stimulating Factors / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004804:Eosinophils; D006801:Humans; D015848:Interleukin-5; D007378:Interleukins / Q000235:genetics*; D008222:Lymphokines / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D013601:T-Lymphocytes
D003115:Colony-Stimulating Factors; D015848:Interleukin-5; D007378:Interleukins; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D004247:DNA; D004262:DNA Restriction Enzymes