title: Genetic rescue of muscle defects associated with a mutant Drosophila melanogaster tropomyosin allele.
abstract: We describe a genetic transformation system which should prove useful for investigating tropomyosin assembly and function. Muscle abnormalities associated with a defective tropomyosin allele were corrected by integrating the wild-type gene into germ line chromosomes. The transformation protocol permits application of directed mutagenesis techniques in investigations of contractile regulatory mechanisms.
authors.affiliation: Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
authors.forename: E A
authors.initials: EA
authors.lastname: Fyrberg
authors.forename: C C
authors.initials: CC
authors.lastname: Karlik
mesh_terms: D000483:Alleles; D000818:Animals; D004262:DNA Restriction Enzymes; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics*; D005796:Genes; D009132:Muscles / Q000033:anatomy & histology; D009154:Mutation; D014335:Tropomyosin / Q000235:genetics*
chemical_list: D014335:Tropomyosin; D004262:DNA Restriction Enzymes