Increasing the Fokl cleavage specificity from 5 to 7 base pairs by two-step methylation.
Pósfai
G
G
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Szybalski
W
W
D010582:Bacteriophage lambda / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D008745:Methylation; D013379:Substrate Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific