[Transformation of the cells of transplantable teratocarcinoma CBA9H6 by a bacterial dihydrofolate reductase gene].
A plasmid containing the bacterial gene of methotrexate-resistant dihydrofolate reductase (dhfr), under the control of early SV40 promoter, was introduced into murine teratocarcinoma CBA9H6 cells. From the whole pool of teratocarcinoma cells, which survived after transient methotrexate selection in vivo, the individual cells were isolated to give rise to 15 clones of tumors. Six of the 15 clones displayed nucleotide sequences of the original vector containing pBR322 sequences and the early SV40 promoter region; however, the bacterial dhfr gene was absent from the transformant clones. Possible causes of the loss of introduced dhfr gene from teratocarcinoma cells under non-selective conditions are discussed.
Tomilin
N V
NV
Titomirov
A V
AV
Stepan'ian
L I
LI
Dyban
P A
PA
D000818:Animals; D001483:Base Sequence; D002999:Clone Cells / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005493:Folic Acid Antagonists; D005798:Genes, Bacterial; D008727:Methotrexate / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012641:Selection, Genetic; D013539:Simian virus 40 / Q000235:genetics; D013724:Teratoma / Q000201:enzymology / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014170:Transformation, Genetic / Q000187:drug effects
D004269:DNA, Bacterial; D004273:DNA, Neoplasm; D005493:Folic Acid Antagonists; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate
Transformatsiia kletok perevivnoĭ teratokartsinomy CBA9H6 bakterial'nym genom digidrofolatreduktazy.