title: Induction of chromosomal aberrations in Chinese hamster ovary cells by the restriction endonuclease Dra I: dose-effect relationships and effect of substitution of chromosomal DNA with bromodeoxyuridine.
abstract: Treatment of G1-phase Chinese hamster ovary (CHO) cells with the restriction endonuclease Dra I (recognition site TTT/AAA) leads to the induction of chromosome-type aberrations. The dose-effect relationships or the frequencies of polycentric chromosomes have a strong linear component. Prelabelling of the cells with bromodeoxyuridine (B) leads to a strong suppression by the chromosome breaking activity of Dra I. This may be explained by assuming that substitution of T by B renders the recognition site of Dra I resistant to being cut by the enzyme.
authors.affiliation: Institut für Genetik, Freie Universität, Berlin, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Obe
authors.forename: C
authors.initials: C
authors.lastname: Johannes
authors.forename: V
authors.initials: V
authors.lastname: Vasudev
authors.forename: P
authors.initials: P
authors.lastname: Kasper
authors.forename: I
authors.initials: I
authors.lastname: Lamprecht
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D004305:Dose-Response Relationship, Drug; D012044:Regression Analysis
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases; D001973:Bromodeoxyuridine