Identification of the bacteriophage D108 kil gene and of the second region of sequence nonhomology with bacteriophage Mu.
To identify the second region of sequence nonhomology between the genomes of the transposable bacteriophages Mu and D108 originally observed by electron-microscopic analysis of DNA heteroduplexes and to localize functions ascribed to the 'accessory' or 'semi-essential' early regions of the phages between genes B and C, a 0.9-kb fragment of each genome located immediately beyond the B gene was cloned and sequenced. Three open reading frames (ORFs) were identified in each. The region of nonhomology is located within the 3' portion of the third ORF. D108 is shown to possess a Kil function similar to that previously shown for Mu, and that function is encoded by the first ORF.
Waggoner
B T
BT
Department of Molecular and Cellular Biology, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Wade
T
T
Pato
M L
ML
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D010583:Bacteriophage mu / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004251:DNA Transposable Elements