Derivation of a murine monoclonal antibody useful for immunohistochemical diagnosis of human adenoid cystic carcinoma and stomach cancer.
A monoclonal antibody, NCC-SG-007, was raised with a formalin-fixed adenoid cystic carcinoma of the salivary gland as the immunogen. The reactivity of the antibody was tested on paraffin sections using the avidin-biotin-peroxidase complex (ABC) method. NCC-SG-007 reacted with 36% of the adenoid cystic carcinomas, 42% of the pleomorphic adenomas and 14% of the mucoepidermoid tumors of the salivary gland which were tested. The antibody also reacted with some normal tissues and various other tumors. Surprisingly, NCC-SG-007 showed a reactivity as high as 94% with gastric carcinomas. The antigenic determinant was revealed to be a carbohydrate chain with no terminal sialic acid, the molecular weight of which was estimated to be 1.5 X 10(6) daltons. This antigen differed from other previously reported gastrointestinal cancer-associated antigens. The antibody should be useful for studying adenoid cystic carcinomas of the salivary gland and gastric cancers.
Kusama
K
K
Department of Pathology, Nihon University School of Dentistry, Tokyo.
Sekine
T
T
Adachi
M
M
Ohgaki
H
H
Hirota
T
T
Morinaga
S
S
Hasegawa
H
H
Hayasaka
N
N
Moro
I
I
Sato
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D003528:Carcinoma, Adenoid Cystic / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D012468:Salivary Gland Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology
D000911:Antibodies, Monoclonal