Cyclic AMP-induced expression of the mouse lactate dehydrogenase-A promoter-cat fusion gene in Chinese hamster ovary wild-type cells, but not in cAMP-dependent protein kinase mutant cells.
The promoter region of mouse lactate dehydrogenase-A gene was fused with the chloramphenicol acetyltransferase gene of Escherichia coli, and the expression of this fusion gene was induced by cyclic AMP in Chinese hamster ovary wild-type cells, but not in mutants affecting the regulatory or catalytic subunit of cAMP-dependent protein kinase.
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D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase; D006224:Cricetinae; D000242:Cyclic AMP / Q000502:physiology*; D005786:Gene Expression Regulation; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000502:physiology*
D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP; D007770:L-Lactate Dehydrogenase; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D011494:Protein Kinases