title: The DEL1 mutator gene in Saccharomyces cerevisiae does not act in trans.
abstract: DEL1 strains of the yeast Saccharomyces cerevisiae exhibit a high rate of deletions of the three linked genes, CYC1, OSM1, and RAD7. Classical genetic methods showed that DEL1 segregated as a single Mendelian gene closely linked to CYC1. In addition, genetic evidence suggested that DEL1 was both cis- and trans-dominant (Liebman et al. 1979). Molecular analysis of deletions isolated from a haploid DEL1 strain established that deletion formation was mediated by recombination between yeast transposable elements, Ty's (Liebman et al. 1981). We now report the molecular characterization of deletions isolated from diploids in the trans configuration. This analysis reveals that these deletions probably arose in a two-step process involving mitotic recombination followed by Ty-mediated deletion formation in cis.
authors.affiliation: Department of Biological Sciences, University of Illinois, Chicago 60680.
authors.forename: S
authors.initials: S
authors.lastname: Picologlou
authors.forename: S W
authors.initials: SW
authors.lastname: Liebman
mesh_terms: D002872:Chromosome Deletion; D003574:Cytochrome c Group / Q000235:genetics; D045304:Cytochromes c; D005796:Genes; D005799:Genes, Dominant; D005800:Genes, Fungal; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c