[Site of integration of IS1-element responsible for expression of the yeast ADE1 gene in Escherichia coli].
Cloned yeast ADE1 gene is expressed in Escherichia coli, due to integration of the IS1 bacterial element into the non-coding 5' region. Primary structure analysis of the integration site of IS1 element demonstrated that this site is situated in a region flanked by two inverted repeats of yeast DNA which are homologous to the right end of the IS1 element. The region up-stream (5') to the left inverted repeat is AT-rich.
Gedminene
G K
GK
Sasnauskas
K V
KV
Ianulaĭtis
A A
AA
D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005800:Genes, Fungal; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004251:DNA Transposable Elements
Issledovanie mesta integratsii IS1-élementa, obespechivaiushchego ékspressiiu gena ADE1 drozhzheĭ v kishechnoĭ palochke.