[Dynamics of the formation of antibodies to surface and internal proteins of the herpes simplex virus in a model experiment on rabbits].
It has been shown by immunoblotting that antibodies to HSV-1 nucleocapsid proteins (39-45K) predominate at the early stages of infection (up to day 21 after infection) in rabbits. At later stages (up to day 75) the intensity of bands corresponding to virus-specific glycoproteins (presumably gD, gE, gC) increases. At the same time the level of antibodies to nucleocapsid proteins diminishes. In ELISA the same pattern was obtained using envelope and nucleo-capsid proteins denatured by SDS and 2-mercaptoethanol. The possibility of using individual HSV antigens for the development of highly specific diagnostic ELISA test-systems is discussed.
Zvonarev
A Iu
AIu
Cheliapov
N V
NV
Ebralidze
L K
LK
Malytseva
N N
NN
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000032:analysis; D002213:Capsid / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006561:Herpes Simplex / Q000276:immunology*; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D011233:Precipitin Tests; D011817:Rabbits; D018139:Simplexvirus / Q000276:immunology*; D013997:Time Factors; D014758:Viral Core Proteins / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins
Dinamika obrazovaniia antitel k poverkhnosti i vnutrennim belkam viru sa gerpesa prostogo v model'nom ékpserimente na krolikakh.