[Development of rational methods for purifying restriction endonucleases].
The work deals with the search for rational schemes of the purification of endonucleases, suitable for the isolation of different enzymes. The scheme using the combination of Blue Sepharose and phosphocellulose has proved to be most universal. The expediency of starting the purification of Haemophilus enzymes on biogel A-0.5m has been established.
Tsvetkova
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D001419:Bacteria / Q000201:enzymology; D002482:Cellulose / Q000031:analogs & derivatives; D002852:Chromatography, Ion Exchange / Q000379:methods; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D005818:Genetic Engineering / Q000379:methods; D012685:Sepharose / Q000031:analogs & derivatives
C029483:Blue-Sepharose; D002482:Cellulose; D012685:Sepharose; C008881:phosphocellulose; D004262:DNA Restriction Enzymes
Razrabotka ratsional'nykh metodov ochistki restriktsionnykh éndonukleaz.