title: Cell culture of the mucinous variant of human colorectal carcinoma.
abstract: Two cell lines, RW-2982 and RW-7213, have been established for the first time from the mucinous variant of human colorectal carcinoma, which is a distinctive and important subtype that has a worse prognosis than the more common nonmucogenic large bowel carcinoma. Methods of establishment and observations made during 7 and 3 years, respectively, of continuous culture are described. These cell lines required 4-9 months of adaptation to tissue culture conditions before noticeable growth occurred. Both cell lines have the following unique properties: (a) growth in vitro as delicate branching three-dimensional tumor particles within a wide gel of insoluble, often translucent mucus (proteoglycan); (b) production of large quantities of carcinoembryonic antigen; (c) ability to survive or adapt to growth in media free of serum, hormones, growth factors, and all protein; and (d) tumorigenicity in multiple sites in nude mice, including liver, with especially rapid growth in the peritoneal cavity as gelatinous material that is nonadherent and noninvasive and thus resembles pseudomyxoma peritonei. Unlike other reported colorectal cell lines, these mucus-coated particulate cell lines will not readily grow as monolayers and grow much more slowly with a doubling time of 2 weeks or more. A serially transplantable tumor from the RW-7213 surgical specimen has also been maintained in nude mice since August 8, 1984. This tumor retains properties of the original specimen. Observations made on the tumor biology of mucogenic colorectal carcinoma using these cell lines are discussed.
authors.affiliation: Department of Pathology, Roger Williams General Hospital, Providence, Rhode Island 02908.
authors.forename: L M
authors.initials: LM
authors.lastname: Tibbetts
authors.forename: M Y
authors.initials: MY
authors.lastname: Chu
authors.forename: M P
authors.initials: MP
authors.lastname: Vezeridis
authors.forename: P G
authors.initials: PG
authors.lastname: Miller
authors.forename: L L
authors.initials: LL
authors.lastname: Tibbetts
authors.forename: M H
authors.initials: MH
authors.lastname: Poisson
authors.forename: P D
authors.initials: PD
authors.lastname: Camara
authors.forename: P
authors.initials: P
authors.lastname: Calabresi
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000473:pathology*; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000032:analysis; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D012004:Rectal Neoplasms / Q000473:pathology*; D013154:Spleen / Q000473:pathology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D002272:Carcinoembryonic Antigen