[A method of isolating plasma membranes from adipose tissue of rats without preliminary treatment with collagenase].
A rapid procedure has been described for preparation of a relatively pure fraction of plasmatic membranes from adipose tissue without treatment with collagenase. Gentle homogenization of rat fatty tissue in a buffered sucrose solution yielded membrane fractions that could be separated from the bulk of contaminating mitochondria and microsomes by a series of differential and isopycnic centrifugations. The preparation obtained was enriched 12-fold with 5'-nucleotidase as compared with the initial homogenate and contained minimal contaminations with mitochondria or elements of endoplasmic reticulum.
Egutkin
G G
GG
D015720:5'-Nucleotidase; D000273:Adipose Tissue / Q000032:analysis* / Q000166:cytology; D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000032:analysis* / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D008297:Male; D003012:Microbial Collagenase; D009708:Nucleotidases / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D009708:Nucleotidases; D015720:5'-Nucleotidase; D003012:Microbial Collagenase
Metod vydeleniia plazmaticheskikh membran iz zhirovoĭ tkani krys bez predvaritel'noĭ obrabotki kollagenazoĭ.