Mechanism of improved maintenance of 2,3-diphosphoglycerate in stored blood by the xanthone compound 2-(2-hydroxyethoxy)-6-(1-H-tetrazole-5-yl)xanthen-9-one (BW A440C).
The effect of the xanthone derivative 2-(2-hydroxyethoxy)-6-(1-H-tetrazole-5-yl)xanthen-9-one (BW A440C) on red cells was studied. When added to stored red cells at a concentration of 6 mM, greatly improved preservation of 2,3-diphosphoglycerate (2,3-DPG) was observed. There was no effect on internal pH of the erythrocyte. At a concentration 0.500 mM, many red cell enzyme activities were inhibited completely. At a 0.500 mM concentration, however, inhibition of pyruvate kinase and diphosphoglycerate phosphatase was most striking. Inhibition of either of these enzymes could result in elevation of 2,3-DPG levels. BW A440C in concentrations which elevated 2,3-DPG levels in humans caused a decrease in 2,3-DPG levels in rabbits and markedly impaired the viability of 21-day stored rabbit erythrocytes.
Beutler
E
E
Research Institute of Scripps Clinic, La Jolla, CA 92037.
Forman
L
L
West
C
C
Gelbart
T
T
D019794:2,3-Diphosphoglycerate; D000818:Animals; D001793:Blood Preservation; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004905:Erythrocyte Aging / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors; D011817:Rabbits; D014966:Xanthenes / Q000494:pharmacology*; D044004:Xanthones
D004163:Diphosphoglyceric Acids; D014966:Xanthenes; D044004:Xanthones; C019794:bisphosphoglycerate phosphatase; D019794:2,3-Diphosphoglycerate; C054768:BWA 440C; D011770:Pyruvate Kinase; D010744:Phosphoric Monoester Hydrolases