title: Parallel determination of mineralocorticoid and glucocorticoid receptors in T- and B-lymphocytes of human spleen.
abstract: The binding characteristics of glucocorticoid and mineralocorticoid receptors were determined in T- and B-lymphocytes from the spleen of four deceased kidney donors. The number of glucocorticoid and mineralocorticoid receptors was equivalent in T- and B-cells (respectively, 1308 +/- 364 and 1335 +/- 520 glucocorticoid receptors per cell, and 174 +/- 29 and 164 +/- 24 mineralocorticoid receptors per cell). The number of binding sites per cell was 5- to 10-fold higher for dexamethasone than for aldosterone. The apparent dissociation constant of dexamethasone for glucocorticoid receptors was 2-fold higher than that of aldosterone for mineralocorticoid receptors. In conclusion, subpopulations of human lymphocytes have an equal number of glucocorticoid and mineralocorticoid receptors.
authors.affiliation: Medizinische Klinik Innenstadt and Kinderklinik, University of Munich, FRG.
authors.forename: D
authors.initials: D
authors.lastname: Armanini
authors.forename: S
authors.initials: S
authors.lastname: Endres
authors.forename: U
authors.initials: U
authors.lastname: Kuhnle
authors.forename: P C
authors.initials: PC
authors.lastname: Weber
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism*; D006801:Humans; D011869:Radioligand Assay; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D011965:Receptors, Glucocorticoid; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid; C028697:dexamethasone receptor