The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.
The use of alternative 5' splice sites in the simian virus 40 early-transcription unit controls the ratio of large T to small t antigen during viral infection. To study the regulation of these alternative 5' splice sites, we made two mutants which improve the match of the large-T-antigen 5' splice site to the 5' splice site consensus sequence. Whether these mutants were assayed in vitro or in vivo, we found that the efficiency of large-T splicing is increased by improving the match of the large-T-antigen 5' splice site to the consensus. We conclude that the match of a 5' splice site is an important determinant of 5' splice site utilization and that the simian virus 40 large-T-antigen 5' splice site is almost certainly recognized by the U1 small nuclear RNA component of the U1 small nuclear ribonucleoprotein particle.
Zhuang
Y
Y
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510.
Leung
H
H
Weiner
A M
AM
D001483:Base Sequence; D005796:Genes; D005814:Genes, Viral; D011494:Protein Kinases / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic
D012322:RNA Precursors; D012342:RNA, Small Nuclear; D011494:Protein Kinases