Benzyl alcohol increases membrane fluidity and modulates cyclic AMP synthesis in intact renal epithelial cells.
To evaluate a possible modulation by membrane fluidity of hormonal, cAMP-mediated effects on renal epithelial cells, we studied the effect of the neutral local anesthetic, benzyl alcohol, on membrane fluidity and on basal and stimulated intracellular cAMP content in intact MDCK cells. Benzyl alcohol induced a dose-dependent decrease of lipid order which was measured by steady-state fluorescence anisotropy using trimethylammonium-diphenylhexatriene and propionyl-diphenylhexatriene as fluorescent probes. Benzyl alcohol induced a 2-fold increase in basal cAMP content, likely as a consequence of increased prostaglandin synthesis since this effect was abolished by indomethacin. The effect of benzyl alcohol on stimulated cAMP synthesis depended on the nature of the ligand: 10 mM benzyl alcohol increased significantly the stimulatory effect of prostaglandin E2, glucagon and forskolin but not of vasopressin. At higher concentrations (40 mM), benzyl alcohol did not affect significantly the glucagon-stimulated cAMP content, while it inhibited significantly the prostaglandin E2-, forskolin- and vasopressin-stimulated cAMP synthesis. The 40 mM benzyl alcohol-induced inhibition was reversed by 1 mM Mn2+, which is known to block the inhibitory GTP-binding protein Ni. These results suggest that: (i) the various components of the adenylate cyclase-cAMP system and their coupling are affected differently by changes in membrane fluidity, which might reflect differences in their lipid environment, (ii) changes in membrane fluidity can modulate responses of renal tubular cells to hormones, and thus tubular functions.
Friedlander
G
G
INSERM U251, Faculté de Médecine Xavier-Bichat, Paris, France.
Le Grimellec
C
C
Giocondi
M C
MC
Amiel
C
C
D000818:Animals; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000494:pharmacology*; D001593:Benzyl Compounds / Q000494:pharmacology*; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D015232:Dinoprostone; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D004848:Epithelium / Q000378:metabolism; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D019204:GTP-Binding Proteins / Q000502:physiology; D005934:Glucagon / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D008560:Membrane Fluidity / Q000187:drug effects*; D011458:Prostaglandins E / Q000494:pharmacology; D014667:Vasopressins / Q000494:pharmacology
D001592:Benzyl Alcohols; D001593:Benzyl Compounds; D005456:Fluorescent Dyes; D011458:Prostaglandins E; D014667:Vasopressins; D004161:Diphenylhexatriene; D005576:Colforsin; D008345:Manganese; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; C037385:1-(4'-carboxyethyl)-6-diphenyl-1,3,5-hexatriene; D005934:Glucagon; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D015232:Dinoprostone; D019905:Benzyl Alcohol; D007213:Indomethacin