Predominant induction of gelsolin and actin-binding protein during myeloid differentiation.
Three actin-associated proteins, actin-binding protein, gelsolin, and profilin, influence gelation, solation, and polymerization, respectively, of actin in vitro. As assessed with specific cDNA probes and immunoaffinity reagents, a 7-50-fold increase in gelsolin, 3-5-fold increase in actin-binding protein, and less than 2-fold increases in actin and profilin protein and mRNA levels accompanied tetradecanoylphorbolacetate-induced differentiation of the myeloid cell lines U937 and HL60 into macrophage-like cells. Such induction in actin-binding protein or gelsolin did not occur in K562 cells, which respond minimally to tetradecanoylphorbolacetate, or following 1,25-dihydroxyvitamin D3-induced monocyte-like differentiation of U937, which results in a less motile phenotype. These observations suggest that increases in gelsolin and actin-binding protein are essential to the expression of many regulated motile functions which takes place during differentiation of myeloid cells.
Kwiatkowski
D J
DJ
Hematology-Oncology Unit, Massachusetts General Hospital, Boston 02114.
D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D002117:Calcitriol / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003285:Contractile Proteins; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D018260:Gelsolin; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000166:cytology; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D051304:Profilins; D012333:RNA, Messenger / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000199:Actins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D003285:Contractile Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; C495738:PFN1 protein, human; D051304:Profilins; D012333:RNA, Messenger; D004247:DNA; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate