title: Characterization of a Pseudomonas aeruginosa transposon insertion mutant with defective release of exoenzymes.
abstract: A Pseudomonas aeruginosa transposon insertion mutant with defective release of several exoenzymes has been characterized. The Tn5-751 insertion mutation was located in the previously described xcp-1 locus at 0 min on the chromosomal map and caused several exoenzymes to remain in cell-bound form. At least one of the exoenzymes, elastase, was accumulated in the periplasmic space. The periplasmic elastase had the same Mr as the extracellular enzyme produced by the wild-type strain. The virulence of the mutant was comparable to that of wild-type strains in experimental burn infection in mice. The presence of an easily selectable antibiotic resistance marker in the xcp-1 locus offers the possibility of cloning the gene(s) involved in exoprotein secretion.
authors.affiliation: Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden.
authors.forename: V
authors.initials: V
authors.lastname: Lindgren
authors.forename: B
authors.initials: B
authors.lastname: Wretlind
mesh_terms: D036002:ADP Ribose Transferases; D000469:Alkaline Phosphatase / Q000378:metabolism; D001427:Bacterial Toxins; D001433:Bacteriolysis; D004251:DNA Transposable Elements; D010450:Endopeptidases / Q000378:metabolism; D005098:Exotoxins / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D009154:Mutation; D010196:Pancreatic Elastase / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D012697:Serine Endopeptidases; D013211:Staphylococcus aureus / Q000187:drug effects; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D000469:Alkaline Phosphatase; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D010450:Endopeptidases; D005098:Exotoxins; D005954:Glucosephosphate Dehydrogenase; D008049:Lipase; D010196:Pancreatic Elastase; D012697:Serine Endopeptidases; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C032923:microbial serine proteinases