title: Affinity gels for purification of retinoid-specific binding protein (RSBP).
abstract: Retinoids are defined as compounds which elicit specific biological effects such as control of cell growth and cell differentiation by binding to a specific receptor. Recently, we demonstrated the presence of a protein (RSBP) which satisfies the criteria for the retinoid receptor. For purification of RSBP, we prepared two types of affinity gels with retinoidal ligands (Gel-Am and Gel-Ch) based on synthetic retinobenzoic acids which possess very potent retinoidal activities. RSBP in the crude fraction extracted from cultured cells could be purified about 300-fold by affinity column chromatography using these affinity gels.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kagechika
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: E
authors.initials: E
authors.lastname: Kawachi
authors.forename: K
authors.initials: K
authors.lastname: Shudo
mesh_terms: D001667:Binding, Competitive; D002352:Carrier Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D005782:Gels; D006801:Humans; D009363:Neoplasm Proteins / Q000302:isolation & purification; D018168:Receptors, Retinoic Acid; D012685:Sepharose / Q000031:analogs & derivatives*; D014212:Tretinoin / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D005782:Gels; D009363:Neoplasm Proteins; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D012685:Sepharose