Probing of DNA polymorphic structure in the cell with osmium tetroxide.
It is shown that osmium tetroxide, 2,2'-bipyridine can be applied as a probe of DNA structure in a bacterial cell. Using this probe we demonstrate (a) presence of structural distortions at the junctions between the right-handed B and left-handed Z DNA in a recombinant plasmid pRW751 and (b) unusual structure of the d(A-T)16 insert in pAT32 plasmid in E. coli cells and in in vitro.
Palecek
E
E
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Rasovská
E
E
Boublíková
P
P
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000187:drug effects / Q000648:ultrastructure*; D004926:Escherichia coli / Q000235:genetics; D009690:Nucleic Acid Conformation; D009992:Osmium / Q000494:pharmacology*; D009993:Osmium Tetroxide / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects
D004269:DNA, Bacterial; D009992:Osmium; D004262:DNA Restriction Enzymes; D009993:Osmium Tetroxide