title: Nucleotide sequences of cDNAs for human papillomavirus type 18 transcripts in HeLa cells.
abstract: HeLa cells expressed 3.4- and 1.6-kilobase (kb) transcripts of the integrated human papillomavirus (HPV) type 18 genome. Two types of cDNA clones representing each size of HPV type 18 transcript were isolated. Sequence analysis of these two types of cDNA clones revealed that the 3.4-kb transcript contained E6, E7, the 5' portion of E1, and human sequence and that the 1.6-kb transcript contained spliced and frameshifted E6 (E6*), E7, and human sequence. There was a common human sequence containing a poly(A) addition signal in the 3' end portions of both transcripts, indicating that they were transcribed from the HPV genome at the same integration site with different splicing. Furthermore, the 1.6-kb transcript contained both of the two viral TATA boxes upstream of E6, strongly indicating that a cellular promoter was used for its transcription.
authors.affiliation: National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Inagaki
authors.forename: Y
authors.initials: Y
authors.lastname: Tsunokawa
authors.forename: N
authors.initials: N
authors.lastname: Takebe
authors.forename: H
authors.initials: H
authors.lastname: Nawa
authors.forename: S
authors.initials: S
authors.lastname: Nakanishi
authors.forename: M
authors.initials: M
authors.lastname: Terada
authors.forename: T
authors.initials: T
authors.lastname: Sugimura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D027383:Papillomaviridae / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D004247:DNA