Strand breakage in DNA by silicic acid.
The alkaline unwinding assay has been used to demonstrate the formation of single-strand breaks in DNA on treatment with silicic acid. Double-stranded DNA, containing no single-strand breaks, when incubated with increasing concentrations of silicic acid, showed the formation of an increasing number of strand breaks per molecule. Experiments on reduction of silicic acid-treated DNA with NaBH4 suggested the possibility of creation of apurinic or apyrimidinic sites. The significance of silicic acid interaction with cellular DNA during asbestos exposure is discussed.
Khan
S G
SG
Industrial Toxicology Research Centre, Lucknow, India.
Rizvi
R Y
RY
Hadi
S M
SM
Rahman
Q
Q
D001894:Borohydrides; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009691:Nucleic Acid Denaturation; D012824:Silicic Acid / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000494:pharmacology*
D001894:Borohydrides; D012824:Silicic Acid; D012822:Silicon Dioxide; D004247:DNA