Improved recovery of nigro-striatally hemitransected rats induced by (Nle4, D-Phe7)alpha-MSH: a central effect.
Rats were subjected to nigro-striatal hemitransection and then intracerebroventricularly infused with the potent and long-acting alpha-MSH analogue, (Nle4, D-Phe7)alpha-MSH, at two different doses (15 or 30 ng/h/rat), or with saline (0.6 microliter/h/rat), continuously for 14 days starting on day 2 after lesion. (Nle4, D-Phe7)alpha-MSH dose-dependently improved the sensorimotor deficit (postural asymmetry, impaired limb reflexes and coordinated limb use, signs of cortical and pyramidal lesion), reduced turning behaviour induced by apomorphine, and increased spontaneous motility in the open field. 3H-Spiperone binding showed that (Nle4, D-Phe7)alpha-MSH treatment caused a down regulation of the striatal DA receptors in the lesioned side, contrary to the supersensitivity developed by the corresponding receptors of saline treated rats. These results indicate that melanopeptides improve the functional recovery of nigro-striatally hemitransected rats, by an action at CNS level.
Zanoli
P
P
Institute of Pharmacology, University of Modena, Italy.
Benelli
A
A
Bertolini
A
A
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D002552:Cerebral Ventricles / Q000187:drug effects / Q000502:physiology*; D003342:Corpus Striatum / Q000502:physiology*; D007276:Injections, Intraventricular; D007700:Kinetics; D008297:Male; D009043:Motor Activity / Q000187:drug effects*; D009412:Nerve Fibers / Q000502:physiology; D011187:Posture; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000187:drug effects / Q000378:metabolism*; D012016:Reference Values; D012018:Reflex / Q000187:drug effects; D013134:Spiperone / Q000378:metabolism; D013378:Substantia Nigra / Q000502:physiology*; D000521:alpha-MSH / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
D011954:Receptors, Dopamine; D013134:Spiperone; D000521:alpha-MSH; C027756:MSH, 4-Nle-7-Phe-alpha-; D001058:Apomorphine