Altered interaction between Sendai virus and a Chinese hamster cell mutant with defective cholesterol synthesis.
An amphotericin B-resistant mutant (AMBr-1) isolated from the Chinese hamster V79 cell line is defective in a pathway for sterol synthesis and contains a much reduced free cholesterol level as compared with the parental V79. The character of the plasma membrane of AMBr-1 was compared with that of V79 by measuring the fusion with the envelope of the Sendai virus and also by measuring membrane fluidity: AMBr-1 was found to be more sensitive to Sendai virus-induced cytolysis than V79. Both assays for membrane-permeability change and electron spin resonance (ESR) study showed an enhanced response to the fusion between viral envelope and plasma membrane in AMBr-1 cells. Measurement of the fluorescence polarization for 1,6-diphenyl-1,3,5-hexatriene suggested that the membrane of AMBr-1 was more fluid than that of V79. This aberrant nature of the cell membrane of AMBr-1 might be caused by the altered membranous sterol content.
Yoshimura
A
A
Department of Biochemistry, Oita Medical School, Japan.
Kobayashi
T
T
Hidaka
K
K
Kuwano
M
M
Ohnishi
S
S
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability; D002784:Cholesterol / Q000172:deficiency / Q000502:physiology*; D006224:Cricetinae; D004161:Diphenylhexatriene; D004578:Electron Spin Resonance Spectroscopy; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D008560:Membrane Fluidity; D008561:Membrane Fusion; D009154:Mutation; D010222:Parainfluenza Virus 1, Human / Q000502:physiology*
D005456:Fluorescent Dyes; D004161:Diphenylhexatriene; D002784:Cholesterol