Cloning of DNA involved in sporulation of Streptomyces griseus.
Twenty-two bald mutants of Streptomyces griseus were isolated and classified into four phenotypic groups, two of which showed conditional sporulation. A 3-kilobase fragment of DNA was cloned in a high-copy-number vector and detected by its ability to restore sporulation to one class of conditionally bald mutants. Analysis of subclones demonstrated that the sporulation property was contained within a 2.5-kilobase fragment. Hybridization studies and restriction analysis indicated that this DNA fragment was present in several Streptomyces species and was distinct from DNA that has been shown to complement afsA mutants of S. bikiniensis and bldA mutants of S. coelicolor.
Babcock
M J
MJ
Department of Microbiology, Ohio State University, Columbus 43210.
Kendrick
K E
KE
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D013171:Spores, Bacterial; D013305:Streptomyces griseus / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI