title: Characterization of single-copy probe from vicinity of centromere of human chromosome 1.
abstract: Human DNA sequences in the human-hamster somatic cell hybrid XJM12.1.3 exist in the form of a minichromosome including the centromere of human chromosome 1. We describe the cloning of XJM12.1.3 DNA into the lambda vector EMBL3, the identification of minichromosome DNA-containing recombinants by hybridization with human sequences, and the characterization of one recombinant as a specific and unique probe for a region close to the centromere of human chromosome 1. This probe and others isolated from the minichromosome DNA are being developed to permit molecular access to a human centromere and its functional sequences.
authors.affiliation: Department of Biology, University of California, San Diego, La Jolla 92093.
authors.forename: J
authors.initials: J
authors.lastname: Solus
authors.forename: A
authors.initials: A
authors.lastname: Jacquemin-Sablon
authors.forename: K
authors.initials: K
authors.lastname: Carine
authors.forename: E
authors.initials: E
authors.lastname: Waltzer
authors.forename: I E
authors.initials: IE
authors.lastname: Scheffler
mesh_terms: D000818:Animals; D002503:Centromere; D002875:Chromosomes; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D006224:Cricetinae; D004262:DNA Restriction Enzymes; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D010957:Plasmids
chemical_list: D004262:DNA Restriction Enzymes