In vitro transformation of osteoblasts: putative formation of osteosarcoma in vitro.
The study of bone cancer has been difficult in part due to a lack of appropriate in vitro osteosarcoma model systems. The development of such systems is essential if a clearer understanding of the biology of and mechanisms behind the formation and progression of bone cancers is to be obtained. We report here the development of an in vitro model system which demonstrates important characteristics generally associated with osteosarcoma. The chick periosteal osteogenesis model was infected with the Fujinami Sarcoma Virus (FSV) containing the v-fps oncogene which encodes for a P140gag-fps protein-tyrosine kinase. Under the appropriate conditions FSV infected cultures developed bone and cartilaginous tissues which showed histopathological findings consistent with osteosarcoma. Biochemical data indicating massive increases in alkaline phosphatase activity, protein content, 3H-Thymidine incorporation as well as expression of active P140gag-fps confirm that transformation has occurred in FSV infected cultures. This novel in vitro model system should prove most useful in the study of bone cancer.
Cogliano
A
A
Mount Sinai Hospital Research Institute, Toronto, Ontario.
Mock
D
D
Birek
C
C
Pawson
A
A
Tenenbaum
H C
HC
D000818:Animals; D001358:Avian Sarcoma Viruses; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology*; D002356:Cartilage / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology*; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D066298:In Vitro Techniques; D015688:Oncogene Protein pp60(v-src); D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology*; D010012:Osteogenesis; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D012191:Retroviridae Proteins / Q000378:metabolism
D012191:Retroviridae Proteins; D015688:Oncogene Protein pp60(v-src)