Selective amplification of the cytoplasmic domain of the epidermal growth factor receptor gene in glioblastoma multiforme.
Primary brain tumors of glial origin often overexpress epidermal growth factor receptors (EGF-Rs). This may be associated with amplification of the EGF-R gene. We have examined tissue from 23 glioblastoma multiforme tumors and found amplification and rearrangement of the EGF-R gene in four of these. The cytoplasmic domain of the EGF-R gene was invariably amplified in these four tumors, while the epidermal growth factor binding domain was not uniformly amplified in three of these tumors. Western blot analysis of the EGF-R protein revealed high levels of a truncated EGF-R protein in two of the four tumors with EGF-R gene amplification.
Malden
L T
LT
Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Victoria, Australia.
Novak
U
U
Kaye
A H
AH
Burgess
A W
AW
D003593:Cytoplasm / Q000032:analysis; D066246:ErbB Receptors / Q000032:analysis / Q000235:genetics*; D005784:Gene Amplification; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D008970:Molecular Weight; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D066246:ErbB Receptors