Reconstitution of a functional interleukin 2 receptor in a nonlymphoid cell.
The maintenance of T lymphocytes which are important effectors of immune responses requires the T cell growth factor, interleukin 2 (IL-2). The binding of IL-2 to specific cell-surface receptors (IL-2R) has previously been shown to be essential to the growth and proliferation of activated lymphocytes. A human IL-2R cDNA sequence, placed under the control of the SV40 transcriptional promoter and enhancer, has been transfected into murine L cells. Single cell analysis by autoradiography was used to show that fibroblastic L cells, stably expressing human IL-2R, respond to stimulation with IL-2 by DNA synthesis and proliferation. This response is specifically blocked by the addition of an anti-IL-2R monoclonal antibody, anti-Tac, as previously reported. Neither nonspecific antisera nor 7G7/B6, an anti-IL-2R monoclonal antibody which does not interfere with IL-2 binding to its receptor, had any effect on this response. The induction of DNA synthesis by IL-2 is both rapid and dose-dependent. The ability of IL-2 to stimulate these transfected L cells to proliferate demonstrates that a lymphoid environment is not required for the functional interaction between IL-2 and its receptor, and provides a unique model system for the investigation of the molecular basis for the cellular events mediated by IL-2.
Rubin
L A
LA
Laboratory of Molecular Virology, National Cancer Institute, Bethesda, MD 20892.
Hoekzema
G S
GS
Nelson
D L
DL
Greene
W C
WC
Jay
G
G
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000235:genetics; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism* / Q000494:pharmacology; D007739:L Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D015375:Receptors, Interleukin-2; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000954:Antigens, Surface; D007376:Interleukin-2; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D004247:DNA