Reexamination of spectroscopic properties of ceruloplasmin freshly isolated with a novel very rapid single-step procedure.
A novel, single-step, chromatographic procedure for the purification of ceruloplasmin is described. The procedure consists of a single chromatographic step, leading in approximately 5 hours from 2 liters of plasma to an electrophoretically homogeneous ceruloplasmin preparation which exhibits different spectroscopic properties with respect to the protein obtained with more lengthy, currently available procedures. The method is suitable for either small or large scale purification from various vertebrate plasma.
Calabrese
L
L
Department of Biochemical Sciences, University of Rome La Sapienza, Italy.
Mateescu
M A
MA
Carbonaro
M
M
Mondovi
B
B
D000818:Animals; D001769:Blood / Q000378:metabolism; D002570:Ceruloplasmin / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003300:Copper / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D013053:Spectrophotometry
D003300:Copper; D002570:Ceruloplasmin