Nicotinic stimulation of polyphosphoinositide turnover in rat adrenal medulla slices.
When rat adrenal medulla slices, treated with atropine (1 X 10(-6) mol X l-1], were stimulated with acetylcholine (1 X 10(-5) mol X l-1), an enhanced 32P incorporation into phosphatidylinositol-bis-phosphate (PIP2) and phosphatidylphosphate (PIP) was observed. On the ather hand, stimulation of myo-[3H] inositol prelabelled slices led to a decline in radioactivity of PIP2, indicating an increased turnover of this phospholipid. A possible affection of monoesterphosphate groups of PIP2 or of both PIP2 and PIP during cholinergic nicotinic stimulation is assumed.
Minenko
A
A
Institute of Drug Research, Academy of Sciences of the GDR, Berlin.
Kiselev
G
G
Tulkova
E
E
Oehme
P
P
D000109:Acetylcholine / Q000378:metabolism; D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D066298:In Vitro Techniques; D007294:Inositol / Q000378:metabolism; D008297:Male; D010716:Phosphatidylinositols / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D011978:Receptors, Nicotinic / Q000187:drug effects*
D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; D011978:Receptors, Nicotinic; D007294:Inositol; D001285:Atropine; D000109:Acetylcholine