title: Efficient processing and expression of human nerve growth factor receptors in Xenopus laevis oocytes: effects on maturation.
abstract: The nerve growth factor (NGF) receptor is an integral membrane protein that is phosphorylated and heavily glycosylated. Determination of the amino acid sequence by molecular cloning indicates that the receptor is a cysteine-rich protein which contains a signal peptide sequence and spans the lipid bilayer with a single transmembrane sequence. A single mRNA of 3.8 kilobases was observed for the receptor, of which 1.5 kilobases is coding sequence. We have used microinjection of receptor RNA in Xenopus laevis oocytes to obtain cell surface expression of the receptor. The presence of NGF receptors in oocytes was verified by radioimmunoassay, specific binding of [125I]NGF, and metabolic labeling followed by immunoprecipitation. The NGF receptor protein was rapidly processed in oocytes and displayed extensive glycosylation. Furthermore, the presence of NGF receptors in oocytes potentiates the ability of progesterone to induce maturation.
authors.affiliation: Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021.
authors.forename: A
authors.initials: A
authors.lastname: Sehgal
authors.forename: D A
authors.initials: DA
authors.lastname: Wall
authors.forename: M V
authors.initials: MV
authors.lastname: Chao
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D009414:Nerve Growth Factors / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D017475:Receptors, Nerve Growth Factor; D011994:Recombinant Proteins / Q000096:biosynthesis; D014982:Xenopus laevis
chemical_list: D009414:Nerve Growth Factors; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D011994:Recombinant Proteins; D011374:Progesterone; D004247:DNA