The corticotropin-releasing factor release in rat hypophysial portal blood is mediated by brain catecholamines.
In order to study the involvement of the hypothalamic corticotropin-releasing factor (CRF) in catecholamine-induced adrenocorticotropin (ACTH) secretion, we have measured CRF levels in rat hypophysial portal blood (HPB) after the pharmacological destruction of the ventral noradrenergic bundle (VNAB), using 6-hydroxydopamine (6-OHDA) stereotaxically injected into the VNAB. CRF levels in HPB were measured by radioimmunoassay, and the effects of 6-OHDA injection were controlled by the determination of catecholamine concentrations in the total hypothalamus. VNAB lesions induced a dramatic decrease in norepinephrine and epinephrine hypothalamic concentration. The CRF levels in HPB were also significantly reduced. These results suggest that central catecholamines exert a direct stimulatory control on the CRF release and play a major role in stress-induced ACTH secretion.
Guillaume
V
V
Conte-Devolx
B
B
Szafarczyk
A
A
Malaval
F
F
Pares-Herbute
N
N
Grino
M
M
Alonso
G
G
Assenmacher
I
I
Oliver
C
C
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D002395:Catecholamines / Q000502:physiology*; D003346:Corticotropin-Releasing Hormone / Q000097:blood*; D004298:Dopamine / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D006892:Hydroxydopamines / Q000494:pharmacology; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology; D009638:Norepinephrine / Q000378:metabolism; D016627:Oxidopamine; D010902:Pituitary Gland / Q000098:blood supply*; D011168:Portal System; D051381:Rats; D011919:Rats, Inbred Strains
D002395:Catecholamines; D006892:Hydroxydopamines; D016627:Oxidopamine; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine