Further characterization of the chondroprogenitor zone in mandibular condyles of suckling mice. An ultrastructural and cytochemical study.
In the neonatal mouse the mandibular condyle serves as an important growth center for the developing mandible. The youngest cells in this organ are the chondroprogenitor cells that are the source for new differentiating chondroblasts. The present study provides new data concerning the fine structure and cytochemical characteristics of the cartilage precursor cells as seen in suckling mice. The condylar chondroprogenitor cells normally reveal a mesenchyme-like appearance with multiple, elongated cell processes that enable close contact between neighboring cells. These cells also exhibit a variety of pinocytotic vesicles, coated pits and appear to be actively involved in the internalization of a fluid-phase marker horseradish peroxidase. Further, the progenitor cells were found to contain trimetaphosphatase reaction products within lysosomal bodies and alkaline phosphatase reactivity along their plasma membrane. Precipitates of calcium complexes in the form of calcium pyroantimonate could be demonstrated in association with the plasmalemma of the cells as well as with the extracellular collagen fibrils. Matrix granules, representing cartilage proteoglycans, became a distinct feature following staining with ruthenium red and were found to be in close contact with the extracellular collagen fibrils and with the plasmalemma. Hence, in addition to their active role in cell proliferation, the progenitor cells are also involved in the synthesis and secretion of major extracellular macromolecules such as collagen and proteoglycans.
Livne
E
E
Laboratory for Musculoskeletal Research, Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine, Haifa, Israel.
Oliver
C
C
Silbermann
M
M
D017766:Acid Anhydride Hydrolases; D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000833:Animals, Suckling; D002356:Cartilage / Q000166:cytology*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008335:Mandibular Condyle / Q000201:enzymology / Q000648:ultrastructure*; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D013234:Stem Cells / Q000201:enzymology / Q000648:ultrastructure*
D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C022768:trimetaphosphatase