Differentiation-associated changes of cation-transport activities in myeloid leukemic cell lines.
Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5-10-fold increase in 45Ca2+ uptake. The increased 45Ca2+ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca2+ uptake in differentiating cells was also demonstrated using the fluorescent probe, fura-2 acetoxymethyl ester. The increased 45Ca2+ uptake was accompanied by a decrease in ouabain-insensitive and -sensitive 86Rb+ uptake. Furthermore, correlation between the changes in these activities was observed. Modulation of extracellular pH affected differentiation: higher pH increased the extent of differentiation.
Rephaeli
A
A
Hematology Division, Beilinson Medical Center, Petach-Tikva, Israel.
Aviram
A
A
Englender
T
T
Shaklai
M
M
D000584:Amiloride / Q000494:pharmacology; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation; D004205:Cromolyn Sodium / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007948:Leukemia, Monocytic, Acute; D015470:Leukemia, Myeloid, Acute; D011188:Potassium / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000494:pharmacology
D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride; D014700:Verapamil; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium; D004205:Cromolyn Sodium; D011188:Potassium; D002118:Calcium