Immunoelectron microscopic localization of mammary tumor virus (MTV) antigens in normal and cancerous mammary glands of mice.
Peroxidase-labeled Fab' fragments of rabbit antisera against gp52 (major envelope protein) and A-particles of mammary tumor virus (MTV) were prepared and used for investigation by immunoelectron microscopy of the replication cycle of MTV-specific envelope and core antigens in normal and malignant mammary gland cells of female mice. The specificity of the antisera was proven by absorption tests and lack of reactivity to MTV-free mammary tissues. Periodate-lysine-paraformaldehyde (PLP) fixation sufficiently preserved the antigenicity of gp52, while Zamboni's fixative was useful to preserve the core antigen. Saponin pretreatment was necessary to reveal the intracellular antigen of A particles but had no influence on gp52. In addition to its presence at the envelope of D particles, gp52 was clearly associated with the biomembrane system, including the nuclear membrane, endoplasmic reticulum, Golgi apparatus and plasma membrane independent of the presence of virus particles. In mammary tumors, a significant level of gp52 antigen was often expressed on the entire cell surface membrane. In contrast, it was localized only to the apical plasma membrane in normal mammary gland cells. A particle antigens were confined to the intracytoplasmic A particles, usually visible as clusters, and to the inner part of B particles. These ultrastructural findings support the available biochemical data on the morphogenesis of MTV particles.
Tsubura
A
A
Department of Pathology, Kansai Medical University, Osaka.
Shikata
N
N
Hilgers
J
J
Zotter
S
S
Tanaka
H
H
Morii
S
S
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D005260:Female; D008321:Mammary Glands, Animal / Q000276:immunology / Q000382:microbiology*; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000382:microbiology*; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D014779:Virus Replication
D000951:Antigens, Neoplasm; D000957:Antigens, Viral, Tumor; C040612:glycoprotein 52 antigen, Mouse mammary tumor virus