title: Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5.
abstract: Conditioned medium from the Con A-treated mouse helper T-cell clone Ly1+2-/9 contains activities that enhance the production of IgA by mouse B cells and induce human cord blood cells to form eosinophil colonies. We have isolated a cDNA sequence that expresses IgA-enhancing factor and eosinophil colony-stimulating factor activities from a cDNA library prepared from activated Ly1+2-/9 cells. Based on homology with the mouse cDNA sequence, a human cDNA sequence coding for an interleukin with IgA-enhancing factor and eosinophil colony-stimulating factor activities was isolated from a cDNA library prepared from a human T-cell clone stimulated with anti-T3 antibody and phorbol 12-myristate 13-acetate. DNA sequence analyses revealed that mouse and human cDNA clones encode proteins of 133 and 134 amino acids, respectively, that are identical to cDNA clones encoding the T-cell replacing factor I and B-cell growth factor II activities. These results establish that a single cDNA clone encodes a protein that acts as a growth and differentiation factor for both B cells and eosinophils.
authors.affiliation: Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94034-1104.
authors.forename: T
authors.initials: T
authors.lastname: Yokota
authors.forename: R L
authors.initials: RL
authors.lastname: Coffman
authors.forename: H
authors.initials: H
authors.lastname: Hagiwara
authors.forename: D M
authors.initials: DM
authors.lastname: Rennick
authors.forename: Y
authors.initials: Y
authors.lastname: Takebe
authors.forename: K
authors.initials: K
authors.lastname: Yokota
authors.forename: L
authors.initials: L
authors.lastname: Gemmell
authors.forename: B
authors.initials: B
authors.lastname: Shrader
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: P
authors.initials: P
authors.lastname: Meyerson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D003001:Cloning, Molecular; D003115:Colony-Stimulating Factors / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004804:Eosinophils; D006801:Humans; D015848:Interleukin-5; D007378:Interleukins / Q000235:genetics*; D008222:Lymphokines / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D013601:T-Lymphocytes
chemical_list: D003115:Colony-Stimulating Factors; D015848:Interleukin-5; D007378:Interleukins; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D004247:DNA; D004262:DNA Restriction Enzymes