Binding of bile salts to fibre-enriched wheat fibre.
A commercial product of fibre-enriched wheat fibre (Fiberform R) was tested for its binding of bile salts in vitro. The wheat fibre preparation was standardized and through enzymatic digestion of protein and starch contained 78 per cent fibre (w/w). Fibre-enriched wheat fibre bound with high capacity both conjugated and unconjugated bile salts. Binding was saturable, reversible and showed no specificity towards tauro- or glycine-conjugated bile salts. Binding was rapid, dependent on pH, was enhanced by the presence of high salt concentrations and partially inhibited by 6 M urea. This indicated that binding was a combination of hydrophobic and hydrophilic interactions.
Florén
C H
CH
Nilsson
A
A
D001647:Bile Acids and Salts / Q000378:metabolism*; D002792:Cholestyramine Resin / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007419:Intestinal Secretions / Q000378:metabolism; D011620:Psyllium / Q000378:metabolism; D013657:Taurodeoxycholic Acid / Q000378:metabolism; D013696:Temperature; D014908:Triticum
D001647:Bile Acids and Salts; D004043:Dietary Fiber; D002792:Cholestyramine Resin; D013657:Taurodeoxycholic Acid; D011620:Psyllium