Rapid preparation of bovine mercaptalbumin by means of covalent chromatography on silica-based materials.
Modified polyvinylimidazole-coated silica materials containing disulphide groups were evaluated for the covalent chromatographic purification of bovine serum albumin. They are inert toward this protein and allow the isolation of 40 mg of mercaptalbumin (0.97-1.05 SH per mole) in less than 3 h. They are easily regenerated.
Millot
M C
MC
Laboratoire de Physico-Chimie des Biopolymères, Université Paris-Val-de-Marne, Creteil, France.
Sebille
B
B
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004220:Disulfides; D004492:Edetic Acid; D012709:Serum Albumin / Q000302:isolation & purification*; D012822:Silicon Dioxide; D013438:Sulfhydryl Compounds / Q000032:analysis
D004220:Disulfides; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; C001702:mercaptoalbumin; D012822:Silicon Dioxide; D004492:Edetic Acid