A lithium-sensitive regulator of sperm flagellar oscillation is activated by cAMP-dependent phosphorylation.
Specific effects of both in vivo activation and in vitro activation by cAMP-dependent phosphorylation on bending wave parameters of demembranated, reactivated, tunicate (Ciona intestinalis) and sea urchin (Lytechinus pictus) sperm flagella can be reversed by exposure to protein phosphatase. The effects of protein phosphatase incubation can be imitated by inclusion of LiCl in the reactivation solutions. The primary effect of cAMP-dependent phosphorylation appears to be activation of a regulatory mechanism controlling flagellar oscillation, rather than activation of the active sliding mechanism. Lithium appears to act on the same regulatory mechanism.
Brokaw
C J
CJ
Division of Biology, California Institute of Technology, Pasadena 91125.
D000818:Animals; D000242:Cyclic AMP / Q000502:physiology; D005407:Flagella / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008094:Lithium / Q000494:pharmacology*; D008297:Male; D010749:Phosphoprotein Phosphatases; D010766:Phosphorylation; D011494:Protein Kinases / Q000502:physiology*; D012617:Sea Urchins; D013081:Sperm Motility / Q000187:drug effects*; D013082:Sperm Tail / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D014561:Urochordata; D014638:Vanadates / Q000494:pharmacology
D014638:Vanadates; D008094:Lithium; D000242:Cyclic AMP; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases