title: Ki-1+ cutaneous lymphoma. Gene rearrangement analysis of tumor cells in tissue and short-term culture of a patient.
abstract: Cutaneous malignant lymphomas developed in an 80-year-old man without any evidence of leukemic disseminations of lymphoma cells. Immunohistologic staining showed the expression of Leu-3a, Leu-4, Ki-1, Tac, and HLA-DR antigens on tumor cells in tissue and large lymphoid cells in long-term, interleukin 2-containing culture of tumor explants. DNA samples extracted from the skin tumors and cultured lymphoid cells showed a clonal rearrangement of T-cell receptor (TCR) gene C beta 1 with the molecular size of 9 kilobases. These findings suggested the T-cell origin of Ki-1+ cutaneous lymphoma cells and the occurrence of a clonal proliferation of tumor cells in culture.
authors.affiliation: Department of Dermatology, Hamamatsu, Japan, University School of Medicine.
authors.forename: H
authors.initials: H
authors.lastname: Sugimoto
authors.forename: F
authors.initials: F
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Yamauchi
authors.forename: Y
authors.initials: Y
authors.lastname: Tokura
authors.forename: K
authors.initials: K
authors.lastname: Iwatsuki
authors.forename: M
authors.initials: M
authors.lastname: Takigawa
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Maeda
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000032:analysis*; D002455:Cell Division; D002999:Clone Cells; D006801:Humans; D007158:Immunologic Techniques; D017730:Ki-1 Antigen; D008223:Lymphoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D011995:Recombination, Genetic; D012878:Skin Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D017730:Ki-1 Antigen; D011948:Receptors, Antigen, T-Cell