title: Identification and characterization of a cytosolic 30 kDa histamine stimulated phosphoprotein in parietal cell cytosol.
abstract: Histamine stimulated acid secretion is mediated by an increase in intracellular cAMP. Cytosolic protein phosphorylation stimulated by histamine was investigated in isolated rabbit parietal cells. Histamine stimulated the phosphorylation of a 30 kDa phosphoprotein with an isoelectric point of 5.6. Cimetidine completely inhibited histamine-stimulated pp30 phosphorylation. However, omeprazole had no effect on the phosphorylation of pp30. Forskolin and 8-bromo-cAMP also stimulated the phosphorylation of pp30. The results suggest that pp30 is a histamine-stimulated, cAMP-dependently phosphorylated protein substrate in parietal cell cytosol.
authors.affiliation: Department of Surgery, Yale University School of Medicine, West Haven, Connecticut.
authors.forename: M
authors.initials: M
authors.lastname: Oddsdottir
authors.forename: J R
authors.initials: JR
authors.lastname: Goldenring
authors.forename: T E
authors.initials: TE
authors.lastname: Adrian
authors.forename: M J
authors.initials: MJ
authors.lastname: Zdon
authors.forename: K A
authors.initials: KA
authors.lastname: Zucker
authors.forename: I M
authors.initials: IM
authors.lastname: Modlin
mesh_terms: D000818:Animals; D002927:Cimetidine / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D003600:Cytosol / Q000032:analysis; D006632:Histamine / Q000494:pharmacology*; D007526:Isoelectric Point; D008970:Molecular Weight; D010295:Parietal Cells, Gastric / Q000032:analysis* / Q000187:drug effects; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D011817:Rabbits
chemical_list: D010750:Phosphoproteins; D002927:Cimetidine; D006632:Histamine; D000242:Cyclic AMP