Proton and iodine-127 nuclear magnetic resonance studies on the binding of iodide by lactoperoxidase.
Interaction of an iodide ion with lactoperoxidase was studied by the use of 1H NMR, 127I NMR, and optical difference spectrum techniques. 1H NMR spectra demonstrated that a major broad hyperfine-shifted signal at about 60 ppm, which is ascribed to the heme peripheral methyl protons, was shifted toward high field by adding KI, indicating the binding of iodide to the active site of the enzyme; the dissociation constant was estimated to be 38 mM at pH 6.1. The binding was further detected by 127I NMR, showing no competition with cyanide. Both 1H NMR and 127I NMR revealed that the binding of iodide to the enzyme is facilitated by the protonation of an ionizable group with a pKa value of 6.0-6.8, which is presumably the distal histidyl residue. Optical difference spectra showed that the binding of an aromatic donor molecule to the enzyme is slightly but distinctly affected by adding KI. On the basis of these results, it was suggested that an iodide ion binds to lactoperoxidase outside the heme crevice but at the position close enough to interact with the distal histidyl residue which possibly mediates electron transport in the iodide oxidation reaction.
Sakurada
J
J
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Takahashi
S
S
Shimizu
T
T
Hatano
M
M
Nakamura
S
S
Hosoya
T
T
D006735:Horseradish Peroxidase / Q000378:metabolism*; D007454:Iodides / Q000378:metabolism*; D007455:Iodine; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010544:Peroxidases / Q000378:metabolism*; D011485:Protein Binding; D011522:Protons; D013053:Spectrophotometry
D007454:Iodides; D011522:Protons; D007455:Iodine; D006735:Horseradish Peroxidase; D010544:Peroxidases