A complex formed between cell components and an HSV structural polypeptide binds to a viral immediate early gene regulatory DNA sequence.
Transcription of HSV immediate early genes is stimulated by a virus structural protein, Vmw65, in a process that requires specific recognition of the sequence TAATGARAT (R = purine). Upon incubation of nuclear extracts of HSV-infected cells with a short DNA fragment containing TAATGARAT, a novel virus-induced protein-DNA complex (named IEC) was detected. Addition of virion extract, containing Vmw65, to nuclear extract from uninfected cells also resulted in the formation of IEC. Vmw65 is a component of IEC, which contains proteins bound specifically to TAATGARAT. Thus, Vmw65 and cellular factors combine to form a sequence-specific DNA-binding complex. This system provides a model for studies of the regulation of inducible cellular genes.
Preston
C M
CM
Medical Research Council Virology Unit, Institute of Virology, Glasgow, Scotland.
Frame
M C
MC
Campbell
M E
ME
D004279:DNA, Viral / Q000378:metabolism*; D015696:Gene Products, tat; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism*
D004279:DNA, Viral; D015696:Gene Products, tat; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D014157:Transcription Factors; D014764:Viral Proteins