Replication of the adeno-associated virus DNA termini in vitro.
The validity of the model proposed by Cavalier-Smith for the replication of linear, single-stranded DNA molecules was tested by using subgenomic DNA termini isolated from adeno-associated virus (AAV), a defective parvovirus. In purified DNA replication systems, the terminal hairpin structure of AAV DNA with a 3'-OH end was shown to prime the synthesis of the daughter DNA strand, which was covalently linked to the parental strand. This resulted in the synthesis of a duplex DNA structure with a terminal hairpin. The availability of such a hairpin structure should facilitate the isolation and characterization of the pivotal enzyme, the putative nickase, for the replication of AAV DNA.
Srivastava
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Department of Medicine, Indiana University School of Medicine, Indianapolis.
D001345:Autoradiography; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D000229:Dependovirus / Q000235:genetics / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D008954:Models, Biological; D014779:Virus Replication
D004279:DNA, Viral