Photoaffinity labeling of a 33 kDa protein subunit of the delta-opioid receptor in neuroblastoma and hybrid cell lines.
Tritiated DTLET (Tyr-D-Thr-Gly-Phe-Leu-Thr) binds with high affinity, specificity and saturability to neuroblastoma N18TG2 and hybrid neuroblastoma x glioma NG108-15 and NG108-5 intact cells. The delta-opioid receptor density in cells cultured in chemically defined medium was increased about 2 times compared to that in cells cultured in 10% fetal calf serum. A major and a minor protein species covalently and specifically bound to [125I]azido-DTLET (Tyr-D-Thr-Gly-pN3Phe-Leu-Thr), photoactivatable ligand, migrated on SDS-gel electrophoresis with Mr values near 33,000 and 58,000, respectively.
Icard-Liepkalns
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Laboratoire de Physiologie Nerveuse, CNRS, Gif-sur-Yvette, France.
Bochet
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D000345:Affinity Labels; D000818:Animals; D001386:Azides / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005910:Glioma / Q000378:metabolism*; D006822:Hybrid Cells / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D010777:Photochemistry; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism*; D017465:Receptors, Opioid, delta; D014316:Tritium; D014407:Tumor Cells, Cultured
D000345:Affinity Labels; D001386:Azides; D007457:Iodine Radioisotopes; D009842:Oligopeptides; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D014316:Tritium; C037653:deltakephalin; C044210:tyrosyl-threonyl-glycyl-(4-azidophenylalanyl)-leucyl-threonine