Age related and lectin influenced changes of exoglycosidases activity in cultured chick embryonic skin fibroblasts.
beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-galactosaminidase and a beta-D-galactosidase activity was determined in untreated and lectins (ConA, PNA, SBA and WGA) treated chick embryonic skin fibroblasts at two incubation stages. Activity of all three glycosidases increased between 7 and 14 incubation days. ConA and WGA affected the levels of enzymatic activity; while SBA and PNA were uneffective. We discuss these findings in relation to a possible role of glycosidases in controlling mesenchymal GAG turnover.
Bodo
M
M
Institute of Histology and general Embryology, University of Perugia, Italy.
Pezzetti
F
F
Becchetti
E
E
Orlacchio
A
A
Evangelisti
R
R
Carinci
P
P
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003208:Concanavalin A / Q000494:pharmacology; D005347:Fibroblasts; D005696:Galactosidases / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism*; D037102:Lectins / Q000494:pharmacology*; D019887:Peanut Agglutinin; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D037121:Plant Lectins; D012867:Skin / Q000166:cytology* / Q000201:enzymology; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins / Q000494:pharmacology; D001616:beta-Galactosidase / Q000378:metabolism*; D048828:beta-N-Acetyl-Galactosaminidase; D010835:Phytohemagglutinins
D000118:Acetylglucosaminidase; D003208:Concanavalin A; D005696:Galactosidases; D006025:Glycosaminoglycans; D006596:Hexosaminidases; D037102:Lectins; D019887:Peanut Agglutinin; D010727:Phosphoric Diester Hydrolases; D037121:Plant Lectins; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; D001616:beta-Galactosidase; D048828:beta-N-Acetyl-Galactosaminidase; C028824:soybean lectin; D010835:Phytohemagglutinins; C026645:N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase