Proteins specified by bovine herpesvirus-2.
Polypeptides synthesized in bovine testes cells infected with bovine herpesvirus type 2 were labeled with [35S]methionine and were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirty-eight virus-induced proteins, ranging in molecular weight from 32,000 to 149,000, were detectable by analysis of whole cell lysates between postinfection hours 2 and 36. A similar number was immunoprecipitated by rabbit antiserum to bovine herpesvirus type 2. Twelve proteins incorporated [3H]glucosamine. On the basis of their temporal characteristics and their pattern of synthesis in the presence of cycloheximide and dactinomycin, 3 proteins, including at least one that was nondetectable in the absence of drugs, were classified as alpha proteins, 4 as beta proteins, 1 as a beta/gamma protein, and 27 as gamma proteins. Host cell protein synthesis was not reduced substantially until postinfection hours 22 to 28.
Adams
S W
SW
Department of Special Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610.
Balachandran
N
N
Hutt-Fletcher
L M
LM
D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006564:Herpesviridae / Q000378:metabolism*; D001911:Herpesvirus 2, Bovine / Q000378:metabolism*; D007118:Immunoassay; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D014764:Viral Proteins