Induction and repression of cellular gene transcription during herpes simplex virus infection are mediated by different viral immediate-early gene products.
Nuclear run-off and pulse-labelling techniques have been used to study the changes in transcription rates of a number of cellular genes during infection with Herpes simplex virus. The majority of these genes show a decrease in transcription rate to about 60% of that observed prior to infection. In contrast, a small number of genes are transcriptionally activated during infection. These effects, which occur at a point in infection after the synthesis of viral proteins but prior to the onset of viral DNA synthesis, are mediated by different immediate-early proteins of the virus. Thus we show that, whilst transcriptional activation requires a functional ICP4 protein, repression is dependent upon the presence of another immediate early protein--ICP22.
Kemp
L M
LM
Department of Biology, University College London, England.
Latchman
D S
DS
D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D004279:DNA, Viral / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D006561:Herpes Simplex / Q000378:metabolism*; D007668:Kidney; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D018139:Simplexvirus / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity; D013489:Suppression, Genetic; D014158:Transcription, Genetic / Q000187:drug effects; D014764:Viral Proteins / Q000494:pharmacology*
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D003513:Cycloheximide; D000212:Acyclovir