title: Brain metabolism and intracellular pH during ischaemia: effects of systemic glucose and bicarbonate administration studied by 31P and 1H nuclear magnetic resonance spectroscopy in vivo in the lamb.
abstract: Brain metabolism and intracellular pH were studied during and after episodes of incomplete cerebral ischaemia in lambs under sodium pentobarbitone anaesthesia. 31P and 1H magnetic resonance spectroscopy was used to monitor brain pHi and brain concentrations of inorganic phosphate (Pi), phosphocreatine (PCr), beta-nucleoside triphosphate (beta NTP), and lactate. Simultaneous measurements were made of arterio-cerebral venous concentration differences (AVDs) for oxygen, glucose, and lactate. Cerebral ischaemia was induced by a combination of bilateral carotid clamping and hypotension, and the acute effects of systemic administration of glucose and sodium bicarbonate were examined. The molar ratio of glucose to oxygen uptake by the brain (6G/O2) increased above unity during cerebral ischaemia. Statistically significant AVDs for lactate were not observed. Cerebral ischaemia was associated with a reduction in brain pHi PCr/Pi ratio, and an increase in brain lactate. No effect of arterial plasma glucose on brain lactate concentration or brain pHi was evident during cerebral ischaemia or in the postischaemic period. Administration of sodium bicarbonate systemically in the postischaemic period was associated with a rise in arterial and brain tissue PCO2. A fall in brain pHi occurred which was attributable in part to coincidental brain lactate accumulation. The increase in brain lactate measured by 1H nuclear magnetic resonance in vivo during ischaemia was insufficient to account for the change in buffer base calculated to have occurred from previous estimates of brain buffering capacity.
authors.affiliation: Department of Paediatrics, School of Medicine, University College London, England.
authors.forename: P L
authors.initials: PL
authors.lastname: Hope
authors.forename: E B
authors.initials: EB
authors.lastname: Cady
authors.forename: D T
authors.initials: DT
authors.lastname: Delpy
authors.forename: N K
authors.initials: NK
authors.lastname: Ives
authors.forename: R M
authors.initials: RM
authors.lastname: Gardiner
authors.forename: E O
authors.initials: EO
authors.lastname: Reynolds
mesh_terms: D000818:Animals; D001158:Arteries; D001639:Bicarbonates / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002550:Cerebral Veins; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D007773:Lactates / Q000097:blood / Q000378:metabolism; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D009711:Nucleotides / Q000378:metabolism; D010101:Oxygen Consumption; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D012756:Sheep; D012964:Sodium / Q000494:pharmacology*; D017693:Sodium Bicarbonate
chemical_list: D001639:Bicarbonates; D001786:Blood Glucose; D007773:Lactates; D009711:Nucleotides; D010710:Phosphates; D010725:Phosphocreatine; D002245:Carbon Dioxide; D019344:Lactic Acid; D017693:Sodium Bicarbonate; D012964:Sodium; D005947:Glucose