A simple and rapid method for the purification of the mitochondrial porin from mammalian tissues.
A new, simple and rapid procedure for the purification of high amounts of mitochondrial porins from different tissues of mammalia is described. The method consists in a single step hydroxyapatite/celite chromatography of Triton X-100 solubilized mitochondrial membranes. For optimal purification several factors are critical such as the absence of salts, a low protein/detergent ratio and an exact hydroxyapatite/celite ratio of 2:1.
de Pinto
V
V
Department of Pharmaco-Biology, University of Bari, Italy.
Prezioso
G
G
Palmieri
F
F
D000818:Animals; D002853:Chromatography, Liquid; D007692:Diatomaceous Earth; D017886:Durapatite; D006882:Hydroxyapatites; D008322:Mammals; D008565:Membrane Proteins / Q000302:isolation & purification*; D008928:Mitochondria / Q000032:analysis*; D017830:Octoxynol; D011092:Polyethylene Glycols; D018272:Porins; D050994:Voltage-Dependent Anion Channels
D006882:Hydroxyapatites; D008565:Membrane Proteins; D018272:Porins; D050994:Voltage-Dependent Anion Channels; D011092:Polyethylene Glycols; D007692:Diatomaceous Earth; D017830:Octoxynol; D017886:Durapatite