Characterization of the product of the cloned fdhF gene of Escherichia coli.
Random insertions of mini-MudII1734 lac gene fusion bacteriophage were constructed on plasmid pLW06, which carries the fdhF gene coding for benzyl-viologen-linked formate dehydrogenase. They allowed us to limit the size of the gene to a 3 kb fragment and to define its direction of transcription. The identification of the fdhF product as a 85 kDa protein was achieved after expression of derivative plasmids in a maxicell system.
Wu
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Laboratoire de Microbiologie, Institut National des Sciences Appliquées, Villeurbanne, France.
Mandrand-Berthelot
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D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005560:Formate Dehydrogenases / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D014176:Protein Biosynthesis
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases