title: Organization of the human pro-alpha 2(I) collagen gene.
abstract: Sixty kilobases of cloned DNA containing the entire human pro-alpha 2(I) collagen gene and 22 kilobases of flanking sequences have been isolated. Like the homologous avian gene, the 1366 amino acid residues of the human pre-pro-alpha 2(I) chain are encoded by 52 exons, whose relative locations and sizes have been determined. Analysis of the 5'- and 3'-untranslated regions have confirmed their exact lengths, as well as conclusively established the nature of five polymorphic mRNA transcripts.
authors.affiliation: Department of Biochemistry, Potchefstroom University, South Africa.
authors.forename: W
authors.initials: W
authors.lastname: de Wet
authors.forename: M
authors.initials: M
authors.lastname: Bernard
authors.forename: V
authors.initials: V
authors.lastname: Benson-Chanda
authors.forename: M L
authors.initials: ML
authors.lastname: Chu
authors.forename: L
authors.initials: L
authors.lastname: Dickson
authors.forename: D
authors.initials: D
authors.lastname: Weil
authors.forename: F
authors.initials: F
authors.lastname: Ramirez
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D011347:Procollagen / Q000235:genetics*
chemical_list: D011347:Procollagen; D004247:DNA; D004262:DNA Restriction Enzymes