title: Expression of glutathione peroxidase I gene in selenium-deficient rats.
abstract: We have characterized a cDNA pGPX1211 encoding rat glutathione peroxidase I. The selenocysteine in the protein corresponded to a TGA codon in the coding region of the cDNA, similar to earlier findings in mouse and human genes, and a gene encoding the formate dehydrogenase from E. coli, another selenoenzyme. The rat GSH peroxidase I has a calculated subunit molecular weight of 22,155 daltons and shares 95% and 86% sequence homology with the mouse and human subunits, respectively. The 3'-noncoding sequence (greater than 930 bp) in pGPX1211 is much longer than that of the human sequences. We found that glutathione peroxidase I mRNA, but not the polypeptide, was expressed under nutritional stress of selenium deficiency where no glutathione peroxidase I activity can be detected. The failure of detecting any apoprotein for the glutathione peroxidase I under selenium deficiency and results published from other laboratories supports the proposal that selenium may be incorporated into the glutathione peroxidase I co-translationally.
authors.affiliation: Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
authors.forename: A P
authors.initials: AP
authors.lastname: Reddy
authors.forename: B L
authors.initials: BL
authors.lastname: Hsu
authors.forename: P S
authors.initials: PS
authors.lastname: Reddy
authors.forename: N Q
authors.initials: NQ
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Thyagaraju
authors.forename: C C
authors.initials: CC
authors.lastname: Reddy
authors.forename: M F
authors.initials: MF
authors.lastname: Tam
authors.forename: C P
authors.initials: CP
authors.lastname: Tu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D051381:Rats; D012643:Selenium / Q000172:deficiency*
chemical_list: D004247:DNA; D005979:Glutathione Peroxidase; D004262:DNA Restriction Enzymes; D012643:Selenium