An internal 5'-noncoding region required for translation of poliovirus RNA in vitro.
A truncated poliovirus RNA that contains the entire 5'-noncoding region as well as some capsid protein-coding sequences was produced from cloned cDNA inserted into an SP6 transcription vector and subsequently was translated in a mixed rabbit reticulocyte-HeLa cell lysate. Deletions or modifications of regions of the 5'-noncoding sequences had significant effects upon the efficiency of translation. The presence of a 60-nucleotide sequence located at positions 567 to 627 appeared to be essential for active ribosome binding and translation of this uncapped RNA.
Bienkowska-Szewczyk
K
K
Department of Cellular Biology, University of Utah School of Medicine, Salt Lake City 84132.
Ehrenfeld
E
E
D002474:Cell-Free System; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
D012333:RNA, Messenger; D012367:RNA, Viral