[Transfection of a plasmid with a retrovirus promoter: distribution of sequences in the genome of induced tumors].
The structure of the integration site of plasmid with LTR of Rous sarcoma virus (pLTR1,5) in the genome of nude mice tumors, induced as a result of N1H3T3 cells' implantation, cotransfected by pLTR1,5 with the DNA of malignant human glioma cells, carrying amplified c-Ha-ras genome, has been studied. The restriction map of the investigated region of the cell genome was obtained. Molecular cloning of the integrated plasmid and adjacent cell sequences has been carried out. It was shown that the exogenic vector in the DNA of the tumor cells is included in BamHI structure of the repeat of mice genome.
Glazachev
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VA
Topol'
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LZ
Tatosian
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AG
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D003001:Cloning, Molecular; D005784:Gene Amplification; D005814:Genes, Viral; D005822:Genetic Vectors; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011519:Proto-Oncogenes; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
Transfektsiia plazmidy s retrovirusnym promotorom: raspredelenie posledovatel'nosteĭ v genome indutsirovannykh opukholeĭ.