title: Transcriptional and post-transcriptional controls establish the cascade of herpes simplex virus protein synthesis.
abstract: Gene expression by herpes simplex virus type 1 (HSV-1) results in the synthesis of three temporal classes of viral proteins. The three classes of viral proteins are expressed in a cascade manner of sequential dependency. The molecular mechanisms that account for the HSV-1 protein synthesis cascade are poorly understood. In order to provide a detailed description of the metabolic levels at which HSV-1 protein synthesis is regulated, we have measured transcription rates and mRNA accumulation levels for 11 HSV-1 genes. These measurements were made over a time-course of infection in the presence or absence of metabolic inhibitors of either viral protein synthesis or viral DNA synthesis. Our observations show that the protein synthesis cascade of HSV-1 is established as a consequence of mechanisms that regulate both the transcription and accumulation of viral messenger RNA.
authors.affiliation: Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210.
authors.forename: S P
authors.initials: SP
authors.lastname: Weinheimer
authors.forename: S L
authors.initials: SL
authors.lastname: McKnight
mesh_terms: D003513:Cycloheximide / Q000494:pharmacology; D004279:DNA, Viral / Q000037:antagonists & inhibitors; D005786:Gene Expression Regulation; D005814:Genes, Viral / Q000187:drug effects; D008957:Models, Genetic; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D003513:Cycloheximide; D010746:Phosphonoacetic Acid