A modified genome electropherotyping procedure for detecting turkey rotaviruses in small volumes of intestinal contents.
A modified extraction procedure, suitable for the quick extraction of viral dsRNA from small quantities of intestinal contents, was developed. Of the 70 specimens positive for dsRNA viruses by genome electropherotyping with preparations derived by the standard extraction procedure, 56 (80%) were positive for virus with preparations derived by the modified procedure.
Theil
K W
KW
Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D007422:Intestines / Q000382:microbiology*; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000235:genetics*; D014422:Turkeys / Q000382:microbiology*
D012330:RNA, Double-Stranded; D012367:RNA, Viral