[Immune response to benzo(a)pyrene in rabbits immunized with a conjugate of benzo(a)pyrene-albumin, synthesized in the microsomal monooxygenase system of the liver].
The feasibility of obtaining a conjugated benz(a)pyrene-protein antigen in the liver cytochrome P-450 system was studied. Covalent binding of benz(a)pyrene (BP) to albumin was performed with the use of liver microsomal fractions of 3-methylcholanthrene-induced rabbits. It was demonstrated that BP oxidation in liver microsomes is accompanied by covalent binding of [14C]BP to exogenous rabbit albumin. Immunization of rabbits with the obtained conjugate results in the development of a specific immune response to BP, and the appearance of specific antibodies and lymphocytes specifically binding [14C]BP in the blood.
Shipulina
N V
NV
Tomilina
N Iu
NIu
Zhirnov
G F
GF
Lisitsyna
V B
VB
Kovalev
I E
IE
Archakov
A I
AI
D000418:Albumins / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D007114:Immunization; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D011817:Rabbits
D000418:Albumins; D001564:Benzo(a)pyrene; D003577:Cytochrome P-450 Enzyme System
Imunnyĭ otvet na benzo(A)piren u krolikov, immunizirovannykh kon''iugatom benzo(A)piren-al'bumin, sintezirovannym v mikrosomalnoĭ monooksigenaznoĭ sisteme pecheni.