Complexes of DNA hairpins and a single-stranded oligonucleotide detected by affinity chromatography and mung bean nuclease cleavage.
The emergence of a simple translation device consisting of an assembler strand (primordial mRNA) and RNA hairpins (primordial tRNA) is presumed to be an important step leading to the origin of life. The assumption of a non-enzymatic interaction of primordial tRNA and mRNA is experimentally approached. DNA hairpins containing five or more adenosine residues in the loop are able to bind to complementary oligonucleotides covalently bound to cellulose. The exact number of base pairs formed between the hairpins and the assembler strand is determined by two methods applied to DNA hairpin/assembler complexes. The melting temperature of a complex is measured and the cleavage pattern by nuclease from mung bean is determined. The loop of the smallest hairpin able to bind consists of five adenosine residues and only three base pairs are formed. This supports the idea of a primordial recognition similar to the contemporary codon-anticodon interaction.
Baumann
U
U
Abteilung Molekularer Systemaufbau, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Federal Republic of Germany.
Lehmann
U
U
Schwellnus
K
K
van Boom
J H
JH
Kuhn
H
H
D005075:Biological Evolution; D002846:Chromatography, Affinity; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D008274:Magnesium; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000235:genetics; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013816:Thermodynamics
D009838:Oligodeoxyribonucleotides; D004247:DNA; D012343:RNA, Transfer; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D008274:Magnesium