Demonstration of Epstein-Barr virus in immunoblastic sarcoma of B-cells arising in a child with primary immunodeficiency disease.
The subject of this investigation was an 11-month-old infant girl who presented with a pathological fracture of the right femur due to a metastasis from an abdominal immunoblastic sarcoma. Her past history included recurrent, intractable bacterial and fungal infections. Investigations of her immune status revealed low numbers of T-lymphocytes, a reversed T-helper (TH)/T-suppressor (TS) cell ratio, no response of her peripheral blood lymphocytes to pokeweed mitogen, phytohemagglutinin, concanavalin A, and Candida albicans, and an inability of her cells to react in a mixed lymphocyte culture. Serum levels of IgG, IgM, and IgA were all below normal. No thymic shadow was visible on the chest radiograph. There was no evidence of adenosine deaminase or nucleoside phosphorylase deficiencies. The tumor cells exhibited both surface IgM and IgG, and many of the cells contained large amounts of cytoplasmic IgM. Light chain specificity was restricted to lambda chain for both surface and cytoplasmic immunoglobulin. Ultrastructural study of the tumor cells revealed the presence of both intranuclear and cytoplasmic virions in roughly 1% of the tumor cells. These viral particles strongly resembled herpes viruses. DNA-hybridization studies on the neoplasm revealed the presence of 7-10 genome equivalents of Epstein-Barr virus-DNA per tumor cell.
Gartner
J G
JG
Murphy
M N
MN
Diocee
M
M
deSa
D J
DJ
McLain
K L
KL
D000008:Abdominal Neoplasms / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D001402:B-Lymphocytes; D004247:DNA; D005260:Female; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006651:Histocytochemistry; D006801:Humans; D007120:Immunochemistry; D007153:Immunologic Deficiency Syndromes / Q000150:complications*; D007223:Infant; D008223:Lymphoma / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization
D004247:DNA