Long terminal repeat (LTR) sequences, env, and a region near the 5' LTR influence the pathogenic potential of recombinants between Rous-associated virus types 0 and 1.
A series of recombinants between Rous-associated virus type 0 (RAV-0), RAV-1, and a replication-competent avian leukosis virus vector (RCAN) have been tested for disease potential in day-old inoculated K28 chicks. RAV-0 is a benign virus, whereas RAV-1 and RCAN induce lymphoma and a low incidence of a variety of other neoplasms. The results of the oncogenicity tests indicate that (i) the long terminal repeat regions of RAV-1 and RCAN play a major role in disease potential, (ii) subgroup A envelope glycoproteins are associated with a two- to fourfold higher incidence of lymphoma than subgroup E glycoproteins, and (iii) certain combinations of 5' viral and env sequences cause osteopetrosis in a highly context-dependent manner. Long terminal repeat and env sequences appeared to influence lymphomogenic potential by determining the extent of bursal infection within the first 2 to 3 weeks of life. This would suggest that bursal but not postbursal stem cells are targets for avian leukosis virus-induced lymphomogenesis. The induction of neutralizing antibody had no obvious influence on the incidence of lymphoma.
Brown
D W
DW
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
Blais
B P
BP
Robinson
H L
HL
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001353:Avian Leukosis / Q000150:complications / Q000382:microbiology*; D001354:Avian Leukosis Virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D001358:Avian Sarcoma Viruses / Q000235:genetics; D002060:Bursa of Fabricius / Q000382:microbiology; D002645:Chickens; D003001:Cloning, Molecular; D008223:Lymphoma / Q000453:epidemiology / Q000209:etiology / Q000662:veterinary; D010022:Osteopetrosis / Q000382:microbiology / Q000662:veterinary; D011201:Poultry Diseases / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology; D012091:Repetitive Sequences, Nucleic Acid; D014766:Viremia / Q000382:microbiology / Q000662:veterinary; D014779:Virus Replication
D000914:Antibodies, Viral