Inhibition of human neutrophil migration by supernatants from Hodgkin's disease-derived cell lines.
The contribution of defective neutrophil function to the increased susceptibility to infection observed in patients with Hodgkin's disease is unclear. We describe cell-directed inhibition of normal human neutrophil migration by serum-free culture supernatants of the Hodgkin-derived cell line L428 KSA, tentatively termed Hodgkin-derived leucocyte factor (HDLF). This factor inhibits both random migration and migration toward different chemoattractants, appears to bind to the cell surface and is stable at 56 degrees C but destroyed at 100 degrees C. Hodgkin-derived leucocyte factor also stimulates basal neutrophil superoxide production but the cells remain fully responsive to n-formyl-methionylleucylphenylalanine. Gel filtration chromatography shows a single peak of migration-inhibitory and superoxide-stimulatory activity at approximately 70,000 g mol-1. Hodgkin-derived leucocyte factor migration inhibition persists in neutrophils from a patient with chronic granulomatous disease. Activity of HDLF is completely destroyed by trypsin but unaffected by the protease inhibitor phenyl-methylsulphonylfluoride. Hodgkin's factor appears to be different from previously described neutrophil migration inhibitory factors.
Schell-Frederick
E
E
Medizinische Universitätsklinik I, Köln, FRG.
Radtke
H
H
Sommer
H
H
Helbing
I
I
Burrichter
H
H
Schaadt
M
M
Diehl
V
V
D002464:Cell Migration Inhibition; D003470:Culture Media; D006689:Hodgkin Disease / Q000473:pathology*; D006801:Humans; D009504:Neutrophils / Q000473:pathology*; D013481:Superoxides / Q000378:metabolism; D014407:Tumor Cells, Cultured
D003470:Culture Media; D013481:Superoxides