The neurotoxicity of ethylcholine mustard aziridinium (ECMA) in rat brain reaggregate cultures.
The cholinergic neurotoxin ECMA causes a biphasic loss of choline acetyltransferase activity in foetal rat whole brain reaggregate cultures. Initial direct inhibition is followed by longer-term loss of cholinergic neurones. Final muscarinic receptor binding, neurofilament protein and Na+, K+-ATPase concentrations suggest that the lesion is specific for cholinergic neurones at 12.5 microM ECMA, but is more generalised at 50 microM ECMA.
Pillar
A M
AM
Department of Pharmacology, King's College (KQC), Strand, London, U.K.
Prince
A K
AK
Atterwill
C K
CK
D000818:Animals; D001388:Aziridines / Q000633:toxicity*; D001389:Azirines / Q000633:toxicity*; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002794:Choline / Q000031:analogs & derivatives* / Q000633:toxicity; D002795:Choline O-Acetyltransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D066298:In Vitro Techniques; D007382:Intermediate Filaments / Q000187:drug effects / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D001388:Aziridines; D001389:Azirines; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; C044894:ethylcholine aziridinium; D002795:Choline O-Acetyltransferase; D000254:Sodium-Potassium-Exchanging ATPase; D002794:Choline