Thyrotropin is a potent growth factor for normal human thyroid cells in primary culture.
We have developed primary cultures of epithelial follicular cells from normal human thyroid tissue, in low serum or serum-free conditions, which allow the in vitro experimentation of the hormonal control of growth. In sharp contrast with several previous studies, thyrotropin (100 microU/ml) potently stimulated the DNA synthesis and proliferation of these cells. These effects were partly reproduced by cyclic AMP agonists. Human thyroid cell proliferation was also increased by serum, epidermal growth factor and a tumor promoting phorbol ester.
Roger
P P
PP
Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Campus Erasme, Belgium.
Dumont
J E
JE
D002478:Cells, Cultured; D000242:Cyclic AMP / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology*
D006133:Growth Substances; D013972:Thyrotropin; D004247:DNA; D000242:Cyclic AMP