title: Neuropeptide-gamma: a peptide isolated from rabbit intestine that is derived from gamma-preprotachykinin.
abstract: The neurokinin A-like immunoreactivity in an extract of rabbit small intestine was resolved into two molecular forms by gel permeation chromatography. These components were purified to apparent homogeneity by reverse-phase HPLC. The primary structure of the larger component was established as the following: Asp-Ala-Gly-His-Gly-Gln-Ile-Ser-His-Lys-Arg-His-Lys-Thr-Asp-Ser-Phe-Val- Gly-Leu - Met.NH2. This amino acid sequence represents residues (72-92) of gamma-preprotachykinin, as predicted from the nucleotide sequence of a cloned cDNA from the rat. The peptide, termed neuropeptide-gamma, lacks residues (3-17) of neuropeptide K, and this segment is specified exactly by exon 4 in the preprotachykinin gene. The smaller form of neurokinin A-like immunoreactivity was identical to neurokinin A. Neuropeptide K was not present in the extract, demonstrating that the pathways of post-translational processing of beta- and gamma-preprotachykinins in the rabbit gut are different.
authors.affiliation: Clinical Research Group for Gastrointestinal Endocrinology, Max-Planck-Gesellschaft, University of Göttingen, F.R.G.
authors.forename: R
authors.initials: R
authors.lastname: Kage
authors.forename: G P
authors.initials: GP
authors.lastname: McGregor
authors.forename: L
authors.initials: L
authors.lastname: Thim
authors.forename: J M
authors.initials: JM
authors.lastname: Conlon
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007421:Intestine, Small / Q000032:analysis*; D008969:Molecular Sequence Data; D015288:Neurokinin A; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D011817:Rabbits; D015320:Tachykinins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D011498:Protein Precursors; D015320:Tachykinins; C044206:preprotachykinin; C059905:tachykinin neuropeptide gamma; D015288:Neurokinin A