Effect of rolling on foci development and viral replication for herpes simplex virus (HSV).
The effect of rolling on the number of herpes simplex virus (HSV) foci and yield of infectious virus was examined for cultures inoculated with clinical specimens. Inoculated cultures were either rolled at 2 revolutions per minute (rpm) or held stationary. Cultures that were rolled at 2 rpm showed a significantly greater number of foci and plaque forming units (PFU) over stationary cultures. The geometric mean fold increase in PFU between rolled and stationary cultures was 8-fold. Rolling of inoculated cultures should be used in the clinical virology laboratory to aid in the rapid detection of HSV.
Mavromoustakis
C T
CT
Department of Medical Microbiology and Immunology, College of Medicine, Ohio State University.
Witiak
D T
DT
Hughes
J H
JH
D002460:Cell Line; D046508:Culture Techniques / Q000379:methods*; D004261:DNA Replication; D006801:Humans; D018139:Simplexvirus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D014779:Virus Replication