Establishment of primate lymphoblastic cell lines by coculture with a simian T-cell leukemia virus-1 positive, hypoxanthine-guanine-phosphoribosyltransferase negative Japanese macaque cell line.
A cell line (JAMH17+) resistant to 8-azaguanine was established from a human T-cell leukemia virus type 1 related virus (simian T-cell leukemia virus-1) positive Japanese macaque cell line. Lymphoblastic cell lines were established from the peripheral blood mononuclear cells of humans, hominoids, and several species of macaques by coculture with JAMH17+ in hypoxanthine-aminopterin-thymidine medium. HTLV-1 specific antigen was detected in some of the established cell lines. Phenotypic analysis showed that several cell lines of crab-eating macaques expressed Leu11a antigen, which is a marker of human natural killer cells.
Murayama
Y
Y
Primate Research Institute, Kyoto University, Aichi, Japan.
Ishida
T
T
Minezawa
M
M
Noguchi
A
A
Takenaka
O
O
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D017977:Deltaretrovirus / Q000276:immunology; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes; D008251:Macaca; D002679:Pan troglodytes; D010641:Phenotype; D011148:Pongo pygmaeus; D012190:Retroviridae / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000956:Antigens, Viral