title: The effect of the cAMP-dependent protein kinase on protein phosphorylation and phosphatidylinositol 4-phosphate formation in a hepatocyte membrane preparation.
abstract: Plasma membrane preparation obtained from isolated mouse hepatocytes was phosphorylated by exogenous cyclic AMP dependent protein kinase in the presence of 32P-ATP. The phosphorylated proteins were analysed by SDS-polyacrylamide gel-electrophoresis of the membrane and the phosphorylated lipids were analysed by thin layer chromatography of the separated lipid fraction. The protein kinase stimulated the 32P incorporation into phosphatidylinositol 4-phosphate and it catalyzed the phosphorylation of several proteins. The main phosphoprotein bands were found at 51 kDa, 49 kDa, 46 kDa and 34 kDa. A part of the 51 kDa phosphoprotein accompanied the lipids in the course of the separation of the lipid fraction.
authors.affiliation: 1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Mészáros
authors.forename: A
authors.initials: A
authors.lastname: Enyedi
authors.forename: G
authors.initials: G
authors.lastname: Bánhegyi
authors.forename: J
authors.initials: J
authors.lastname: Mandl
authors.forename: F
authors.initials: F
authors.lastname: Antoni
authors.forename: A
authors.initials: A
authors.lastname: Faragó
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
chemical_list: D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D011506:Proteins; C037178:phosphatidylinositol 4-phosphate; D011494:Protein Kinases