Genetic reassortment of bluetongue virus serotype 11 strains in the bovine.
Reassortants of bluetongue virus Serotype 11 (BTV-11) were isolated from a yearling heifer experimentally infected with two electrophoretically different strains (UC-2 and UC-8) by subcutaneous inoculation. Viruses were recovered by direct titration of sonicated blood samples onto Vero cell monolayers, which were overlaid with agarose and later plaque purified. The parental electropherotype of UC-8 was identified as the predominant virus strain during the infection; UC-2 was not isolated. UC-2 infectivity was shown by reassortants which contained genome segments that were identical in migration pattern to the parental UC-2 electropherotype. The observations demonstrate that segmental reassortment can occur during mixed infections in the bovine, between strains of the same BTV serotype.
Oberst
R D
RD
Department of Veterinary Pathology, University of California, Davis 95616.
Stott
J L
JL
Blanchard-Channell
M
M
Osburn
B I
BI
D000818:Animals; D001819:Bluetongue / Q000382:microbiology*; D001820:Bluetongue virus / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005814:Genes, Viral; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000235:genetics*; D014709:Vero Cells; D014766:Viremia / Q000382:microbiology / Q000662:veterinary
D012330:RNA, Double-Stranded; D012367:RNA, Viral