[Use of the RI plasmid to study radiation damage to the methyl anti-nuclease barrier in the DNA of Escherichia coli].
Ionizing radiation (gamma-rays, 100 to 750 Gy) does not impair methyl anti-nuclease barrier in DNA of exposed cells. From this it follows that the methyl anti-nuclease barrier is radioresistant. It is assumed that restrictases are involved in degradation of single-strand DNA regions, that is, in degradation of replicative forks.
Torosian
M V
MV
Rabinkova
E V
EV
Aĭzenberg
O A
OA
Naumova
L A
LA
Shishkova
O V
OV
D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors*; D004269:DNA, Bacterial / Q000528:radiation effects*; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000201:enzymology / Q000528:radiation effects; D005720:Gamma Rays; D008745:Methylation; D011815:R Factors; D011836:Radiation Tolerance
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI
Ispol'zovanie plazmidy RI dlia izucheniia radiatsionnogo narusheniia metil'nogo protivonukleaznogo bar'era DNK u Escherichia coli.