Modulation of leucocyte activation in the early phase of the rabbit burn injury.
Previous studies in burned patients have shown an early enhanced polymorphonuclear leucocyte (PMN) generating capacity for superoxide radical (O2.-), for the arachidonic acid (AA) lipoxygenase metabolite leukotriene B4 (LTB4) and for platelet activating factor-acether (PAF). These findings have been confirmed on a burn injury rabbit model. As we have suggested a pivotal role for an exaggerated initial (less than 36-48 h) neutrophil stimulation leading to a later (greater than 72 h) immuno-depression and anergy, we tried to modulate the early phase by drug therapy. A Ginkgo biloba extract (IPS200) injected i.v. in burned rabbits greatly reduced O2.- and LTB4 generation on A23187 challenge. IPS200 includes flavonoids and other polyphenols, inhibiting either arachidonic acid metabolism or PAF receptors, and may thus exert their modulating effect on PMN function in thermal injury.
Lavaud
P
P
Centre de Recherches, Service de Santé des Armées, Clamart, France.
Mathieu
J
J
Bienvenu
P
P
Braquet
M
M
Gerasimo
P
P
Kergonou
J F
JF
Ducousso
R
R
D000818:Animals; D002056:Burns / Q000188:drug therapy / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D004195:Disease Models, Animal; D004224:Diterpenes; D005609:Free Radicals; D046934:Ginkgolides; D007783:Lactones / Q000627:therapeutic use*; D007975:Leukotriene B4 / Q000096:biosynthesis; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D011817:Rabbits; D013481:Superoxides / Q000378:metabolism
D004224:Diterpenes; D005609:Free Radicals; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D013481:Superoxides; D007975:Leukotriene B4; D000001:Calcimycin; C045856:ginkgolide B