Purine and pyrimidine metabolism in human muscle and cultured muscle cells.
Using radiochemical methods, we determined the activities of various enzymes of purine and pyrimidine metabolism in homogenates of human skeletal muscle and of cultured human muscle cells. Results show a large discrepancy between the enzyme activities in muscle and cultured cells. With regard to purine metabolism, adenylate (AMP) deaminase activity was only 1-3% in cultured cells compared to that in muscle, whereas the activity of adenosine deaminase, purine-nucleoside phosphorylase, adenosine kinase, adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase was 7-15-fold higher in the cultured cells. The enzymes of pyrimidine metabolism, orotate phosphoribosyltransferase, orotidine 5'-monophosphate decarboxylase and uridine kinase showed activity of 100-200-fold higher in cultured cells than in adult muscle. The differences in enzyme activity are probably related to the low differentiation stage and the absence of contractile activity in the cultured muscle cells. Care must be taken when using these cells as a model for studying purine and pyrimidine metabolism of adult myofibers.
Jacobs
A E
AE
Department of Biochemistry, University of Nijmegen, The Netherlands.
Oosterhof
A
A
Veerkamp
J H
JH
D015720:5'-Nucleotidase; D000659:AMP Deaminase / Q000378:metabolism; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000243:Adenosine Deaminase / Q000378:metabolism; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D006801:Humans; D008875:Middle Aged; D009132:Muscles / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*
D011687:Purines; D011743:Pyrimidines; C010192:9-(2-hydroxy-3-nonyl)adenine; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D000659:AMP Deaminase; D000225:Adenine