Differential regulation of c-Ha-ras and c-Ki-ras gene expression in rat mammary gland.
Steady-state levels of c-Has-ras and c-Ki-ras RNA in gestating and lactating rat mammary gland were measured. The c-Ha-ras-specific RNA level increased during gestation and declined with the onset of lactation. On the contrary, the level of c-Ki-ras RNA remained unchanged both during gestation and lactation. There was no change in the level of ras-transcripts in either growing or regressing 7,12-dimethylbenz[a]anthracene-induced mammary tumors. Intravenous infusion of prolactin or implantation of pituitary-derived tumor, which secretes mammotropic hormone, in virgin female rats resulted in decreased levels of c-Ha-ras RNA and no change in c-Ki-ras RNA levels. Our results suggest the transcription of c-Ha-ras and c-Ki-ras genes is differentially regulated.
Nieto
A
A
Laboratory of Molecular Virology, NCI.
Qasba
P K
PK
Nakhasi
H L
HL
Dhar
R
R
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015247:Deoxyribonuclease HindIII; D005260:Female; D005786:Gene Expression Regulation; D007774:Lactation; D008321:Mammary Glands, Animal / Q000032:analysis*; D008325:Mammary Neoplasms, Experimental / Q000235:genetics; D009857:Oncogenes; D011247:Pregnancy; D012334:RNA, Neoplasm / Q000032:analysis; D051381:Rats
D012334:RNA, Neoplasm; D015127:9,10-Dimethyl-1,2-benzanthracene; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII