title: The molybdenum-pterin binding protein is encoded by a multigene family in Clostridium pasteurianum.
abstract: There are three variants of the molybdenum-pterin binding protein (Mop) encoded by three distinct genes in Clostridium pasteurianum. Nucleotide sequence analysis shows that the three mop genes have greater than 90% homology at the nucleotide level. Upstream from the coding region of each mop gene are potential promoter consensus sequences. Analysis of Mop purified from cells grown under nitrogen-fixing conditions indicates all three genes are expressed. Sequence analysis of the three mop genes and the gene products predicts that there are 10 amino acid replacements among the family. The amino acid replacements are chemically conservative accounting for the co-purification of the three variants of Mop. Protein chemistry data suggest the possibility that glutamic acid residues in Mop may be modified in vivo.
authors.affiliation: Cold Spring Harbor Laboratories, NY 11724.
authors.forename: S M
authors.initials: SM
authors.lastname: Hinton
authors.forename: C
authors.initials: C
authors.lastname: Slaughter
authors.forename: W
authors.initials: W
authors.lastname: Eisner
authors.forename: T
authors.initials: T
authors.lastname: Fisher
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003013:Clostridium / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C050771:mopI protein, Clostridium pasteurianum; D004262:DNA Restriction Enzymes