Transformation of Balb 3T3 cells by the BNLF-1 gene of Epstein-Barr virus.
The BNLF-1 protein is the only non-nuclear Epstein-Barr virus (EBV) encoded protein that has been detected in B-lymphocytes immortalized by EBV. We demonstrate that the BNLF-1 gene induces anchorage-independent growth and tumorigenic transformation of the murine cell-line, Balb/3T3. This demonstration extends the earlier observation that the BNLF-1 gene can transform Rat-1 cells. In addition we find that the BNLF-1 protein is located in the particulate fraction of cells, is phosphorylated, and is turned over with a half-life of 2.0 to 3.5 h in the BNLF-1 transformed Balb/3T3 cells, just as it is in EBV-genome-positive B-cell-lines.
Baichwal
V R
VR
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Sugden
B
B
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D010750:Phosphoproteins / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism; D014763:Viral Matrix Proteins
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins