Presence of a 43-kDa host-cell polypeptide in purified aphthovirions.
A 43kDa cellular polypeptide (P43), which comigrates with host-cell actin in both SDS-PAGE and isoelectrofocusing slab gels, was found associated to 140 S aphthoviral particles purified from BHK 21 cells labeled with [35S]methionine prior to infection. Ultracentrifugation analysis of disrupted virions demonstrates that polypeptide P43 is not associated to VP1-3 containing 12 S subunits but remains, like viral polypeptide VP4, at the top of the sucrose gradients. In addition, in vitro iodination or trypsin treatment show that P43 is protected from the action of both procedures and therefore supports the hypothesis that host-cell polypeptide P43 is located within the viral particles.
Grigera
P R
PR
Centro de Virologia Animal (CEVAN-CONICET), Serrano, Argentina.
Tisminetzky
S G
SG
Lebendiker
M B
MB
Periolo
O H
OH
La Torre
J L
JL
D000199:Actins / Q000032:analysis; D000818:Animals; D005537:Aphthovirus / Q000032:analysis*; D006224:Cricetinae; D007526:Isoelectric Point; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D014771:Virion / Q000032:analysis
D000199:Actins; D011506:Proteins