Transposon mutagenesis in Bordetella avium.
Transposon mutagenesis was used to introduce specific mutations in the chromosome of Bordetella avium. The transposon Tn5 was transferred by conjugation to B avium from Escherichia coli strain SM10 by the suicide plasmid vector pSUP1011. A defined minimal medium for growth of B avium was developed for scoring transposon-containing mutants for specific nutritional requirements. Approximately 1% of all mutants tested were auxotrophic in some form, and these organisms were grouped into 13 classes. Also, 2 classes of hemagglutination-negative mutants were isolated. Stability of Tn5 insertion was examined, and frequencies of reversion less than 10(-7) were observed, indicating stable mutations.
Leyh
R D
RD
Department of Veterinary Microbiology, Iowa State University, Ames 50011.
Griffith
R W
RW
Arp
L H
LH
D001884:Bordetella / Q000235:genetics*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation
D004251:DNA Transposable Elements; D004269:DNA, Bacterial