title: Demonstration of 1,25(OH)2 vitamin D3 receptors and actions in vascular smooth muscle cells in vitro.
abstract: Binding of [3H] 1,25(OH)2D3 and effects of 1,25(OH)2D3 on cell ultrastructure were evaluated in vascular smooth muscle cells (VSMC) primary cultures (aortic media). Specific reversible binding of [3H] 1,25(OH)2D3 by a 3.5 S macromolecule with DNA binding, KD 6.2 X 10(-10) M and Nmax 16 fmol/mg protein was demonstrated. Incubation of VSMC with 10(-8) M 1,25(OH)2D3, but not 25(OH)D3, in the presence of 10% FCS for up to three weeks caused rapid reversible appearance in the cytoplasm of membrane-bounded electron-dense lysosomal particles which on electronspectroscopic imaging contained Ca and Pi. VSMC are targets for vitamin D.
authors.affiliation: Dept. Internal Medicine and Pathology, University of Heidelberg, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Merke
authors.forename: W
authors.initials: W
authors.lastname: Hofmann
authors.forename: D
authors.initials: D
authors.lastname: Goldschmidt
authors.forename: E
authors.initials: E
authors.lastname: Ritz
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism*
chemical_list: D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D002117:Calcitriol