Sindbis virus infection decreases intracellular pH: alkaline medium inhibits processing of Sindbis virus polyproteins.
Infection of baby hamster kidney cells by Sindbis virus, an alphavirus, resulted in a decrease in the intracellular pH of approximately 0.5 units within the first 1-2 hr after infection as measured either by equilibrium labeling with [14C]benzoic acid or by use of a pH-sensitive fluorescent probe, 2,7-bis-carboxyethyl-5,6-carboxyfluorescein-acetooxymethyl ester. In contrast, intralysosomal pH, as measured using an endocytized pH-sensitive probe, fluorescein isothiocyanate-labeled dextran, was not altered by Sindbis virus infection. Production of Sindbis virus was reduced by more than 90% and post-translational processing of Sindbis virus envelope precursors was inhibited in infected cells incubated in alkaline medium.
Moore
L L
LL
Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112.
Bostick
D A
DA
Garry
R F
RF
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000502:physiology; D008970:Molecular Weight; D011188:Potassium / Q000502:physiology; D011499:Protein Processing, Post-Translational; D012845:Sindbis Virus / Q000254:growth & development*; D014036:Togaviridae Infections / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014779:Virus Replication
D014759:Viral Envelope Proteins; D011188:Potassium