Opioid peptide profile in human pituitary.
The comprehensive metabolic profile of endogenous opioid peptides is established here for human pituitary for the first time. Sixteen human pituitaries, obtained postmortem, were analyzed individually by gradient reversed-phase high-performance liquid chromatography together with a radio-receptor assay with [3H]etorphine as ligand. This combination was used to detect opioid receptor activity. The 16 assay profiles were sufficiently consistent for a composite of them to serve as a comparative basis for other studies on the pathophysiology of the human pituitary. To demonstrate one selected comparison, we present data on a distinctively different profile of opioid receptor activity in the pituitary of one patient who died from a drug overdose.
Desiderio
D M
DM
Department of Neurology, University of Tennessee, Memphis 38163.
Fridland
G H
GH
Francisco
J T
JT
Sacks
H
H
Robertson
J T
JT
Cezayirli
R C
RC
Killmar
J
J
Lahren
C
C
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D004723:Endorphins / Q000378:metabolism*; D005048:Etorphine / Q000378:metabolism*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009019:Morphinans / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D011041:Poisoning / Q000378:metabolism; D011869:Radioligand Assay; D011957:Receptors, Opioid / Q000378:metabolism*
D004723:Endorphins; D009019:Morphinans; D011957:Receptors, Opioid; D005048:Etorphine