Vaccination of rabbits with a bovine herpesvirus type 1 subunit vaccine: adjuvant effect of ISCOMs.
Bovine herpesvirus type 1 has two major immunogenic surface glycoproteins: a 90 kDa haemagglutinin and the 130 (74 + 54) kDa glycoprotein. These proteins were purified by rate zonal sucrose density gradient centrifugation after extraction with nonionic detergent Triton X-100. For the preparation of ISCOMs, the glycoproteins were further adsorbed during a second rate zonal centrifugation, to micelles of Quil A glycoside, already added to the gradient. Haemagglutinating peaks were collected and used as subunit vaccine in rabbits: groups of three animals were injected with 50, 10 and 5 micrograms protein. Seroconversion was followed by ELISA, haemagglutination inhibition, neutralizing and plaque reduction assays. ISCOMs and unadsorbed subunits were efficient in inducing neutralizing as well as haemagglutination inhibiting antibodies: ISCOMs gave a higher level of response. The efficiency demonstrated by ISCOMs suggests their potential as a subunit vaccine.
Trudel
M
M
Centre de Recherche en Virologie, Institut Armand-Frappier, Université du Québec, Ville de Laval, Canada.
Nadon
F
F
Seguin
C
C
Boulay
G
G
Lussier
G
G
D000276:Adjuvants, Immunologic / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D006384:Hemagglutination; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D008823:Micelles; D011817:Rabbits; D014763:Viral Matrix Proteins / Q000302:isolation & purification*; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D008823:Micelles; D014763:Viral Matrix Proteins; D014765:Viral Vaccines