Site-directed mutagenesis of the polyomavirus genome: replication-defective large T mutants with increased immortalization potential.
We used the deletion loop mutagenesis procedure to direct point mutations into a small region of the polyomavirus genome, at 0.97 map units, affecting the structure of both the middle and large T antigens. We describe the construction of six middle T mutants which have retained the ability to transform rat cells in culture and four large T mutants, three of which are deficient in viral DNA replication and capable of immortalizing primary rat embryo fibroblasts very efficiently.
Roberge
C
C
Department of Microbiology, University of Sherbrooke, Quebec, Canada.
Bastin
M
M
D000957:Antigens, Viral, Tumor / Q000235:genetics*; D002472:Cell Transformation, Viral; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D011120:Polyomavirus / Q000235:genetics*; D013329:Structure-Activity Relationship; D014779:Virus Replication
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral