A double-label method detects both early (T-antigen) and late (capsid) proteins of JC virus in progressive multifocal leukoencephalopathy brain tissue from AIDS and non-AIDS patients.
A new double-label immunocytochemical method detects JC virus (JCV) early (T-antigen) and late (capsid) proteins simultaneously in cryostat sections of progressive multifocal leukoencephalopathy (PML) brain tissue from both acquired immunodeficiency syndrome (AIDS) and non-AIDS patients. T-antigen is detected with a monoclonal antibody (PAb 416) followed by goat anti-mouse IgG and mouse Clono-PAP, while capsid proteins are detected by a rabbit polyclonal antiserum to capsid proteins followed by biotinylated goat anti-rabbit IgG and streptavidin-alkaline phosphatase conjugate. The substrates are 3,3'-diaminobenzidine and Vector Red I, respectively. With this method some infected glial cells stain for late (capsid) antigens in the nucleus, while others show early protein (large T-antigen) immunoreactivity. The latter are likely to be astrocytes infected abortively or oligodendrocytes in the early stages of a productive JCV infection.
Stoner
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GL
Laboratory of Experimental Neuropathology, NINCDS, National Institutes of Health, Bethesda, MD 20892.
Soffer
D
D
Ryschkewitsch
C F
CF
Walker
D L
DL
Webster
H D
HD
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002213:Capsid / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007577:JC Virus / Q000276:immunology*; D007968:Leukoencephalopathy, Progressive Multifocal / Q000382:microbiology* / Q000473:pathology; D009836:Oligodendroglia / Q000382:microbiology; D011120:Polyomavirus / Q000276:immunology*
D000957:Antigens, Viral, Tumor