title: A neutral sphingomyelinase in spermatozoal plasma membranes.
abstract: A highly active neutral sphingomyelinase was observed for the first time in ram spermatozoal plasma membranes. The optimal conditions for the enzyme activity are pH 7.4, 40 mM MgCl2, 40 min of incubation, and 267 nmol sphingomyelin. Ca2+ and cholesterol were found to inhibit sphingomyelinase activity.
authors.affiliation: Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
authors.forename: V T
authors.initials: VT
authors.lastname: Hinkovska
authors.forename: D H
authors.initials: DH
authors.lastname: Petkova
authors.forename: K S
authors.initials: KS
authors.lastname: Koumanov
mesh_terms: D002462:Cell Membrane / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013094:Spermatozoa / Q000201:enzymology*; D013108:Sphingomyelin Phosphodiesterase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase