Host cell-induced differences in O-glycosylation of herpes simplex virus gC-1. I. Structures of nonsialylated HPA- and PNA-binding carbohydrates.
Lectins with narrow oligosaccharide specificities were established as probes to study the host cell influence on the biosynthesis of O-linked oligosaccharides of the herpes simplex virus type 1 (HSV-1)-specified glycoprotein C (gC-1). We found that only gC-1 and no other glycoprotein bound to the peanut lectin (PNA), with main specificity for Gal(beta 1-3)GalNAc. Previously, we have shown that only gC-1 binds to the Helix pomatia lectin (HPA), with main specificity for terminal GalNAc. The O-linked oligosaccharides binding to PNA and HPA were released by alkaline borohydride treatment and characterized. A structural determination of these oligosaccharides showed that the HPA-binding carbohydrates were monosaccharides (GalNAc), and that the PNA-binding oligosaccharides were disaccharides with the structure Gal-GalNAc. The PNA- and HPA-binding oligosaccharides were arranged as Pronase-resistant clusters on gC-1, consisting of about seven individual, adjacent oligosaccharides. In addition to these disaccharides, Pronase-resistant PNA-binding glycopeptides of gC-1 also contained neutral trisaccharides. Larger O-linked oligosaccharides, binding to the wheat germ lectin, were found in gC-1, but not in proximity to the PNA-binding ones. It was concluded that the lectins mentioned should be useful probes in screening HSV-infected cells of different lineages for differences in processing of O-linked oligosaccharides.
Lundström
M
M
Department of Clinical Virology, University of Göteborg, Sweden.
Olofsson
S
S
Jeansson
S
S
Lycke
E
E
Datema
R
R
Månsson
J E
JE
D002460:Cell Line; D006031:Glycosylation; D037102:Lectins; D009844:Oligosaccharides / Q000032:analysis; D019887:Peanut Agglutinin; D011402:Pronase; D011499:Protein Processing, Post-Translational; D018139:Simplexvirus / Q000254:growth & development*; D014415:Tunicamycin / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000378:metabolism*
C038095:Helix lectin; D037102:Lectins; D009844:Oligosaccharides; D019887:Peanut Agglutinin; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D014415:Tunicamycin; D011402:Pronase