Crystallization of purified recombinant human interleukin-1 beta.
The gene for human interleukin-1 beta was cloned from SK-hep-1 hepatoma cellular RNA and expressed at high levels in Escherichia coli both as the naturally processed form (rIL-1 beta) and as a variant with an additional sequence of three amino acids on the N-terminus (rIL-1 beta +). Expressed protein was purified to homogeneity by a sequence of steps, which included low pH incubation, adsorption and desorption from Procion Red Sepharose, sizing on a Superose 12 fast-performance liquid chromatography (FPLC) column, and anion exchange chromatography on QAE Sepharose. The final step provided a biologically active protein that migrates on two-dimensional (2-D) gels as a single spot with a pI of 6.7 +/- 0.2 and a molecular mass of 17,500 daltons. Concentrated solutions of rIL-1 beta have produced crystals by ammonium sulfate precipitation. The crystals are tetragonal, show the symmetry of space group P4(1) or its enantiomer, have lattice constants of a = 58.46 (1) and c = 77.02 (3) A, and scatter to at least 2 A resolution. A structure determination based on these crystals is under way.
Carter
D B
DB
Molecular Biology Research, Upjohn Company, Kalamazoo, Michigan 49007.
Curry
K A
KA
Tomich
C S
CS
Yem
A W
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Deibel
M R
MR
Tracey
D E
DE
Paslay
J W
JW
Carter
J B
JB
Theriault
N Y
NY
Harris
P K
PK
D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D003001:Cloning, Molecular; D003460:Crystallization; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000302:isolation & purification*; D008113:Liver Neoplasms; D010957:Plasmids; D011994:Recombinant Proteins / Q000302:isolation & purification*
D007375:Interleukin-1; D011994:Recombinant Proteins; D004262:DNA Restriction Enzymes