title: Pathogenesis of experimental reovirus tenosynovitis in chickens: influence of the route of infection.
abstract: Four groups of specific pathogen-free, day-old chicks were infected experimentally with an avian arthrotropic reovirus strain R2 by four different routes:--oral, subcutaneous, foot-pad and intra-articular. These groups were followed sequentially to study: pathological changes in the hock joints and liver; cloacal virus shedding and the presence of virus in hock joints; serological responses as determined by enzyme linked immunosorbent assay (ELISA), agar gel precipitation (AGP) and virus neutralization tests. All 4 infected groups developed arthritis or tenosynovitis with synovial hyperplasia and lymphocytic infiltration. Foot-pad and intra-articular routes of infection were found to advance the disease process by 2 to 3 weeks after infection by these routes were associated with superficial degenerative changes in articular cartilage. Antibodies were detected at 2 to 3 weeks p.i. by all 3 methods, but there were no significant differences between the patterns of serological response in the infected groups. Injection into the foot-pad appears to be the most convenient and effective parenteral route of experimental infection.
authors.affiliation: University of Liverpool, Department of Veterinary Pathology, Leahurst, Neston, South Wirral, U.K.
authors.forename: M R
authors.initials: MR
authors.lastname: Islam
authors.forename: R C
authors.initials: RC
authors.lastname: Jones
authors.forename: D F
authors.initials: DF
authors.lastname: Kelly
mesh_terms: D000818:Animals; D002645:Chickens; D007267:Injections; D007596:Joints / Q000473:pathology; D011201:Poultry Diseases / Q000209:etiology; D012087:Reoviridae; D012088:Reoviridae Infections / Q000662:veterinary; D012699:Serology / Q000379:methods; D013717:Tenosynovitis / Q000209:etiology / Q000662:veterinary*; D014765:Viral Vaccines / Q000008:administration & dosage
chemical_list: D014765:Viral Vaccines