Investigations on myelinogenesis in vitro: II. The occurrence and regulation of protein kinases by thyroid hormone in primary cultures of cells dissociated from embryonic mouse brain.
The occurrence and regulation by thyroid hormone of four protein kinases (cyclic AMP independent and dependent, calcium/calmodulin stimulated, and calcium/phosphatidyl serine stimulated protein kinases) was studied in primary cultures of cells dissociated from embryonic mouse brain. Serum from a thyroidectomized calf, which contained low levels of L-3,5,3'-triiodothyronine, T3 (less than 25 ng/100 ml), and thyroxine, T4 (less than 1 microgram/100 ml) was used in the culture medium in place of normal calf-serum (T3, 130 ng/100 ml; T4 5.9 micrograms/100 ml) to render the cultures responsive to exogenously added T3. Cultures grown in hypothyroid calf-serum containing medium had less cAMP dependent and independent protein kinase activity than control cultures grown in normal calf-serum containing medium. However, this activity was restorable to a considerable degree if the cultures grown in hypothyroid calf serum containing medium were supplemented with L-3,5,3'-triiodothyronine (T3). The presence of calcium/calmodulin stimulated protein kinase was also distinctly observed. In comparison, the activity of calcium/phosphatidyl serine stimulated protein kinase was less than the other protein kinases.
Shanker
G
G
Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Pieringer
R A
RA
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology; D002147:Calmodulin / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000502:physiology; D004075:Diglycerides / Q000378:metabolism; D004789:Enzyme Activation; D066298:In Vitro Techniques; D051379:Mice; D009186:Myelin Sheath / Q000502:physiology*; D010718:Phosphatidylserines / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D012995:Solubility; D014284:Triiodothyronine / Q000494:pharmacology*
D002147:Calmodulin; D004075:Diglycerides; D010718:Phosphatidylserines; D014284:Triiodothyronine; D000242:Cyclic AMP; D011494:Protein Kinases