Analysis and isolation of cytomegalovirus DNA by field inversion gel electrophoresis.
High molecular weight human cytomegalovirus (CMV) DNA was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. A high yield of CMV DNA molecules was obtained within 1 week of infecting the cell culture. Labelling of the viral DNA with biotin by nick translation enabled the detection of CMV-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.
van den Berg
F M
FM
Department of Pathology, University of Amsterdam, The Netherlands.
Jiwa
M
M
Rook
R
R
Geelen
J L
JL
D000311:Adrenal Glands / Q000382:microbiology; D003587:Cytomegalovirus / Q000032:analysis* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000382:microbiology; D004279:DNA, Viral / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral