title: Synthesis of decadeoxyribonucleotides containing 5-modified uracils and their interactions with restriction endonucleases Bgl II, Sau 3AI and Mbo I (nucleosides and nucleotides 82).
abstract: Decadeoxyribonucleotides containing uracil, 5-bromouracil, 5-cyanouracil and 5-ethyluracil in recognition sequences of restriction endonucleases Bgl II, Sau 3AI, Mbo I were synthesized. Decanucleotides containing 5-bromouracil in place of thymine had essentially the same susceptibility to all the restriction endonucleases. Uracil-containing decanucleotides were however very resistant to attack. Decanucleotides containing 5-cyanouracil in the recognition sequence were strongly resistant to hydrolysis by Sau 3AI, but were hydrolysed by Bgl II and Mbo I as well as the parent decanucleotide. Decanucleotides containing 5-ethyluracil were strongly resistant to hydrolysis by Sau 3AI, but were partially resistant to hydrolysis by Bgl II and Mbo I.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: A
authors.initials: A
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Ueda
mesh_terms: D001426:Bacterial Proteins; D001976:Bromouracil; D002942:Circular Dichroism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014498:Uracil / Q000031:analogs & derivatives*
chemical_list: D001426:Bacterial Proteins; D003854:Deoxyribonucleotides; C048011:5-ethyluracil; C056431:5-cyanouracil; D001976:Bromouracil; D014498:Uracil; D004262:DNA Restriction Enzymes; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases