Forskolin inhibition of glucose transport in bone cell cultures through a cAMP-independent mechanism.
Studies are presented demonstrating inhibition of glucose transport by forskolin in human MG-63 osteosarcoma cells as well as osteoblast-like cells derived from normal human trabecular bone and chick calvaria. The cAMP-stimulators parathyroid hormone, prostaglandin E2, and isoproterenol did not influence glucose transport. Benzyl alcohol, a membrane lipid fluidity modulator, also provoked inhibition of the glucose uptake rate. Effects of forskolin and benzyl alcohol were not additive. It is suggested that cAMP is not a mediator of glucose transport in bone cells, and that forskolin inhibits glucose transport via a cAMP-independent mechanism.
van Valen
F
F
II. Medízinische Klinik und Poliklinik C, Universität Düsseldorf, West Germany.
Keck
E
E
D000818:Animals; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D001842:Bone and Bones / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D002645:Chickens; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D003847:Deoxyglucose / Q000493:pharmacokinetics; D015232:Dinoprostone; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D010281:Parathyroid Hormone / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology
D001592:Benzyl Alcohols; D010281:Parathyroid Hormone; D011458:Prostaglandins E; D005576:Colforsin; D003847:Deoxyglucose; D000242:Cyclic AMP; D005947:Glucose; D015232:Dinoprostone; D007545:Isoproterenol; D019905:Benzyl Alcohol