Lipocortin-like 33 kDa protein of guinea pig neutrophil. Its distribution and stimulation-dependent translocation detected by monoclonal anti-33 kDa protein antibody.
33 kDa protein of neutrophil is a lipocortin-like protein, as proposed from its biochemical properties, amino acid composition, and the homology of its amino acid sequence to human lipocortin I. The localization and translocation of 33 kDa protein (p33) in blood cells of guinea pig were studied by immunoblotting (Western blotting) and immunocytochemical fluorescence methods using polyclonal and monoclonal mouse anti-p33 antibodies. The protein was determined to be present only in the cytoplasm of neutrophils, but not in cells such as the monocyte, lymphocyte, platelet, and other bone marrow cells. The translocation of the protein from cytoplasm to cell membrane was coupled with the increase in intracellular calcium ion and in superoxide generation induced by a chemotactic factor. These findings suggest that p33 may have an important role not only in the regulatory mechanism of phospholipase A2 (PLA2) activity but also in other transmembrane signaling.
Sato
E F
EF
Department of Medical Biology, Kochi Medical School, Japan.
Okimasu
E
E
Takahashi
R
R
Miyahara
M
M
Matsuno
T
T
Utsumi
K
K
D000818:Animals; D017302:Annexins; D000911:Antibodies, Monoclonal; D001798:Blood Proteins / Q000032:analysis*; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006023:Glycoproteins / Q000097:blood* / Q000378:metabolism; D006168:Guinea Pigs; D008213:Lymphocyte Activation; D051379:Mice; D008970:Molecular Weight; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000032:analysis* / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D017302:Annexins; D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D003574:Cytochrome c Group; D006023:Glycoproteins; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium