title: Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector.
abstract: We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formation in the presence of geneticin sulfate (G-418). The value of AAV as an alternative to the retrovirus vector systems is discussed.
authors.affiliation: Department of Pathology, University of Florida, Gainesville 32610.
authors.forename: D
authors.initials: D
authors.lastname: LaFace
authors.forename: P
authors.initials: P
authors.lastname: Hermonat
authors.forename: E
authors.initials: E
authors.lastname: Wakeland
authors.forename: A
authors.initials: A
authors.lastname: Peck
mesh_terms: D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D000229:Dependovirus / Q000235:genetics*; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D014170:Transformation, Genetic