title: Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN.
abstract: We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always greater than or equal to 99% with 90% recovery of the monocytes from the mononuclear cells.
authors.affiliation: Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0602.
authors.forename: M C
authors.initials: MC
authors.lastname: Vissers
authors.forename: S A
authors.initials: SA
authors.lastname: Jester
authors.forename: J C
authors.initials: JC
authors.lastname: Fantone
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D003102:Colloids; D003973:Diatrizoate; D005362:Ficoll; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes; D009000:Monocytes; D011134:Polysaccharides; D012822:Silicon Dioxide
chemical_list: D003102:Colloids; D011134:Polysaccharides; D003973:Diatrizoate; D005362:Ficoll; D012822:Silicon Dioxide