Kininase II in bronchoalveolar lavage fluid and serum of patients with pulmonary disorders.
In 68 patients with pulmonary diseases such as lung cancer (n = 32), sarcoidosis (n = 21) and chronic bronchitis (n = 15), kininase II (EC 3.4.15.1) (KII) was fluorimetrically (F-LKII) and the albumin concentration (LALB) spectrophotometrically measured in native unconcentrated bronchoalveolar lavage fluid. For comparison in serum, the KII was also spectrophotometrically (SP-SKII) and fluorimetrically (F-SKII) determined. In all patients, the mean (means) +/- SD F-LKII activity was 0.2 +/- 0.4 U/L, the LALB concentrations 0.17 +/- 0.44 g/L, and the resulting specific F-LKII activity was 3 +/- 3 U/g LALB. The highest elevations of LALB concentrations were determined in lung cancer. The highest activities of F-LKII, F-SKII, and SP-SKII were observed in sarcoidosis. A significant linear relation was found between F-LKII and LALB (R = 0.60, P less than 0.0002) and also between F-SKII and SP-SKII (R = 0.88, P less than 0.0001), but no relation could be established between F-SKII and F-LKII, indicating that the KII measured in lavage was not identical with the KII found in serum.
Schweisfurth
H
H
Medical Clinic, Marienhospital Gelsenkirchen, Federal Republic of Germany.
Leppert
R
R
D001991:Bronchitis / Q000097:blood / Q000201:enzymology; D005260:Female; D006801:Humans; D008171:Lung Diseases / Q000097:blood / Q000201:enzymology*; D008175:Lung Neoplasms / Q000097:blood / Q000201:enzymology; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000097:blood / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000201:enzymology*; D012507:Sarcoidosis / Q000097:blood / Q000201:enzymology; D012709:Serum Albumin / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods; D007507:Therapeutic Irrigation
D012709:Serum Albumin; D007703:Peptidyl-Dipeptidase A