Synthesis in Escherichia coli of the HTLV-I trans-acting protein p40x.
The pX gene of human T-cell leukemia virus type I (HTLV-I) encodes a protein that activates the expression of viral genes in trans. Plasmid constructs designed to express the pX gene under the control of either the temperature-inducible lambda PL promoter or the trp promoter were used to transform several Escherichia coli strains, including murein-lipoprotein and Ion mutant strains. Upon induction it was possible to detect the synthesis of a new polypeptide of approximately 40 kDa which reacted specifically with serum from an ATL patient.
Sánchez
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A
Department of Molecular Genetics, Nippon Roche Research Center, Kanagawa, Japan.
Richardson
M A
MA
Yoshida
M
M
Furuichi
Y
Y
D003001:Cloning, Molecular; D017977:Deltaretrovirus / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli; D005786:Gene Expression Regulation; D007163:Immunosorbent Techniques; D009687:Nuclear Proteins / Q000235:genetics*; D010957:Plasmids; D012191:Retroviridae Proteins / Q000235:genetics* / Q000276:immunology; D014157:Transcription Factors / Q000235:genetics*
D009687:Nuclear Proteins; D012191:Retroviridae Proteins; D014157:Transcription Factors