Prostaglandins and the tumor-dependent growth inhibitory activity of Yoshida ascites fluid.
The content of prostaglandins in Yoshida tumor ascites fluid was evaluated by extraction of the arachidonic acid metabolites by means of octadecylsilyl-silica chromatography (ODS-extracts). The pattern of association of the icosanoids to ascites fluid colloids was determined by sequential precipitation with polyethylene glycol and extraction of the precipitates with methanol (M-extracts). Thin layer chromatographic analysis of these extracts demonstrated in the ODS-extract the presence of chromatographic fractions corresponding to prostaglandin E2 (4.6 micrograms/mL) and prostaglandin A2 (3.0 micrograms/mL), while in the M-extract only the fraction corresponding to prostaglandin A2 (9.2 micrograms/mL) was detected. This fraction was as the growth inhibitory activity reduced by approximately 60% in M-extracts from animals treated with Indomethacin. These results support previous indications that arachidonic acid metabolites are involved in the cytostatic activity of ascites fluid extracts. The preferential association of prostaglandin E2 to the ascites macroglobulin fraction could be a consequence of the presence of an unidentified tumor-dependent amphiphilic polymer in the Yoshida ascites fluid.
Krøll
J
J
Department of Clinical Chemistry, Rigshospitalet, University of Copenhagen, Denmark.
D000818:Animals; D001202:Ascitic Fluid / Q000032:analysis* / Q000503:physiopathology; D002455:Cell Division; D002855:Chromatography, Thin Layer; D004261:DNA Replication; D015232:Dinoprostone / Q000302:isolation & purification*; D008055:Lipids / Q000302:isolation & purification; D008114:Liver Neoplasms, Experimental / Q000503:physiopathology*; D011454:Prostaglandins A / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D008055:Lipids; D011454:Prostaglandins A; C100008:prostaglandin A2; D015232:Dinoprostone