Expression of the functional D-glucose transport system in Xenopus oocytes injected with mRNA of rat small intestine.
mRNA prepared from rat small intestine was injected into Xenopus oocytes. The injected oocytes showed a clear electrical response to D-glucose (Glu) in the form of membrane depolarization and conductance increase, while none was shown to D-fructose. The membrane electrical response of the injected oocytes evoked by Glu followed the Michaelis-Menten type kinetics and was dependent on the membrane potential of the oocyte. Replacing the Na+ of the bathing buffer with choline+ resulted in no response to Glu. Thus, a Glu transport system coupled to a Na+ gradient was expressed in Xenopus oocytes by injecting mRNA from rat small intestine.
Aoshima
H
H
Department of Chemistry, Faculty of Liberal Arts, Yamaguchi University, Japan.
Ishii
H
H
Anan
M
M
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C027786:glucose receptor; D005947:Glucose