Intragenic cis-acting art gene-responsive sequences of the human immunodeficiency virus.
The art gene product of the human immunodeficiency virus is required for the expression of virion capsid and envelope gene proteins. The experiments presented here show that sequences located within the coding region of the envelope gene exert a negative effect on the expression of heterologous genes and that the negative effect of these sequences can be relieved by the art gene product. This region in the env gene contains negative regulatory sequences that inhibit gene expression, as well as a sequence necessary for the art gene product-dependent relief of repression. The experiments define the cis- and trans-acting components of a regulatory system that permits differential expression of human immunodeficiency virus virion structural and regulatory proteins.
Rosen
C A
CA
Dana-Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115.
Terwilliger
E
E
Dayton
A
A
Sodroski
J G
JG
Haseltine
W A
WA
D002213:Capsid / Q000096:biosynthesis; D005786:Gene Expression Regulation; D015694:Gene Products, rev; D015696:Gene Products, tat; D005814:Genes, Viral; D006678:HIV / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics*; D014764:Viral Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus