Importance of virus passage history on the growth of Coxsackie B4 in human skin fibroblasts.
Human skin fibroblasts have previously been reported to display an age-dependent resistance to infection with coxsackie B4 virus. We have shown that the virus will replicate and produce CPE in human skin fibroblasts regardless of the age of the donor of the cells. The passage history of the virus was found to influence the titre of the virus in these cells.
Miller
M
M
State Health Laboratory Services, Queen Elizabeth II Medical Centre, Nedlands, Australia.
Harnett
G B
GB
Bucens
M R
MR
D000328:Adult; D000818:Animals; D003588:Cytopathogenic Effect, Viral; D029822:Enterovirus B, Human / Q000254:growth & development*; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008828:Microbiological Techniques; D012692:Serial Passage; D012867:Skin / Q000166:cytology; D014709:Vero Cells; D014779:Virus Replication