title: Residual bodies in the retinal pigment epithelium induced by intravitreal netilmicin.
abstract: Intravitreal injection of aminoglycoside antibiotics is known to induce morphological changes in the retinal pigment epithelium (RPE) resembling a lipidosis. The RPE of netilmicin-treated rabbits displays a dose-related increase in autofluorescence compared to untreated controls. Netilmicin produces an accumulation of membrane-limited osmiophilic lamellated inclusions in the pigment epithelial cell. These inclusions measure from 1 to 3 microns in diameter, and have acid hydrolase activity demonstrated by cytidine monophosphate cytochemistry. These findings suggest that netilmicin-induced inclusions are residual bodies and that the accumulation of these residual bodies is responsible for the observed cellular lipidosis.
authors.affiliation: Howe Laboratory of Ophthalmology, Harvard Medical School, Boston, Massachusetts.
authors.forename: C A
authors.initials: CA
authors.lastname: Tabatabay
authors.forename: D J
authors.initials: DJ
authors.lastname: D'Amico
authors.forename: L A
authors.initials: LA
authors.lastname: Hanninen
authors.forename: V N
authors.initials: VN
authors.lastname: Casey
authors.forename: K R
authors.initials: KR
authors.lastname: Kenyon
mesh_terms: D000818:Animals; D006651:Histocytochemistry; D006867:Hydrolases / Q000378:metabolism; D002479:Inclusion Bodies / Q000201:enzymology / Q000648:ultrastructure*; D007267:Injections; D008856:Microscopy, Fluorescence; D009428:Netilmicin / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000648:ultrastructure*; D011817:Rabbits; D012160:Retina / Q000201:enzymology; D014822:Vitreous Body
chemical_list: D009428:Netilmicin; D006867:Hydrolases