title: UDP-N-acetylglucosamine: lysosomal enzyme precursor N-acetylglucosamine-1-phosphate transferase activities in human ovarian tumor tissue and some transformed cell lines.
abstract: Uridine diphosphate-N-acetylglucosamine: lysosomal enzyme precursor N-acetyl-glucosamine-1-phosphate transferase, is a key enzyme involved in the intracellular targeting of lysosomal enzymes. This enzyme is elevated fourfold in primary ovarian tumor microsomes with respect to normal ovarian microsomes. This elevation is associated with significant increases in the specific activity of multiple lysosomal hydrolases, including beta-D-hexasaminidase, alpha-L-fucosidase, and beta-D-galactosidase. The activity of the phosphotransferase was also documented in several cell lines derived from human tumors. The possible role of this enzyme in tumor-associated phosphorylation is discussed.
authors.affiliation: Department of Gynecologic Oncology, Roswell Park Memorial Institute, New York State Department of Health, Buffalo 14263.
authors.forename: R
authors.initials: R
authors.lastname: Madiyalakan
authors.forename: O T
authors.initials: OT
authors.lastname: Mueller
authors.forename: T B
authors.initials: TB
authors.lastname: Shows
authors.forename: K L
authors.initials: KL
authors.lastname: Matta
mesh_terms: D005260:Female; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000201:enzymology*; D010770:Phosphotransferases / Q000032:analysis*; D017855:Transferases (Other Substituted Phosphate Groups); D014407:Tumor Cells, Cultured / Q000201:enzymology
chemical_list: D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C030012:UDP-N-acetylglucosamine-lysosomal-enzyme-N-acetylglucosaminephosphotransferase