A latex agglutination test for the detection of canine parvovirus and corresponding antibodies.
Latex particles coated with rat monoclonal antibodies directed against a canine parvovirus are agglutinated by the virus antigens. In this study, the reaction was quantitated by a technique which had been described as immunoassay by particles counting. The method is as sensitive as a radioimmunoassay with the same antibody, but has the advantages of simplicity and safety. It allows the detection of parvoviral antigens to 4 ng/ml. Incubation of these antigens with specific antibodies resulted in inhibition of the agglutination reaction. By this procedure the presence of antibodies against canine parvovirus can be detected to a concentration of 150 ng/ml. The system can be easily automated, thus increasing reproducibility. This latex agglutination technique can be performed as a slide test; and although this method is 4-5 times less sensitive, it could be useful in field studies, for the detection of the viral antigens and the corresponding antibodies.
Bodeus
M
M
Unit of Microbiology, University of Louvain (U.C.L.), Belgium.
Cambiaso
C
C
Surleraux
M
M
Burtonboy
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control; D004285:Dogs; D007841:Latex Fixation Tests / Q000379:methods*; D008828:Microbiological Techniques; D010321:Parvoviridae / Q000276:immunology* / Q000302:isolation & purification; D010322:Parvoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D051381:Rats; D014765:Viral Vaccines / Q000627:therapeutic use
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014765:Viral Vaccines