title: A DNA sequence outside the pUB110 minimal replicon is required for normal replication in Bacillus subtilis.
abstract: The origin of lagging strand synthesis in pUB110, oriL, has been localized within 140 bases outside the pUB110 minimal replicon. The oriL DNA sequence is a cis-acting and orientation dependent determinant required for normal plasmid replication. Rearrangements affecting oriL cause plasmid instability, lead to the accumulation of replication intermediates and result in a marked reduction of the plasmid copy number in some recombination deficient mutant strains. In addition, deletion of oriL triggers a dnaB-dependent mode of replication. Insertion of the functionally asymmetric oriL region in the proper orientation into pC194 reduces the accumulation of single-stranded DNA during the replication of this plasmid.
authors.affiliation: Max-Planck-Institut für Molekulare Genetik, Berlin, FRG.
authors.forename: J F
authors.initials: JF
authors.lastname: Viret
authors.forename: J C
authors.initials: JC
authors.lastname: Alonso
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D010957:Plasmids; D012093:Replicon; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
chemical_list: D004262:DNA Restriction Enzymes