Isolation of a cDNA clone for transition protein 1 (TP1), a major chromosomal protein of mammalian spermatids.
We have isolated a cDNA clone for rat transition protein 1 (TP1), a major chromosomal protein of mammalian spermatids. The clone was identified initially by hybrid selection of TP1 mRNA. The sequence of the 251-nucleotide cDNA includes the entire coding region for the protein, thereby confirming the identity of the clone as well as predicting two changes in the published amino acid sequence.
Heidaran
M A
MA
Department of Chemistry, University of South Carolina, Columbia 29208.
Kistler
W S
WS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013087:Spermatids / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D012333:RNA, Messenger; C053886:spermatid transition proteins; D004247:DNA; D004262:DNA Restriction Enzymes