Immunoaffinity-purified opiate receptor specifically binds the delta-class opiate receptor ligand, cis-(+)-3-methylfentanylisothiocyanate, SUPERFIT.
Using an antibody generated against the opiate receptor on NG108-15 cells, we recently purified the putative receptor from this hybrid cell line. We herein report that the purified receptor complex specifically binds tritiated cis-(+)-3-methylfentanylisothiocyanate (SUPERFIT), with the predominant binding associated with a 58 kDa polypeptide chain. Consistent with these findings is the in situ labeling of a 58 kDa protein with [3H]SUPERFIT on NG108-15 cells.
Carr
D J
DJ
University of Alabama at Birmingham, Department of Physiology and Biophysics 35294.
DeCosta
B
B
Jacobson
A E
AE
Bost
K L
KL
Rice
K C
KC
Blalock
J E
JE
D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D005283:Fentanyl / Q000031:analogs & derivatives* / Q000378:metabolism; D007158:Immunologic Techniques; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D011957:Receptors, Opioid / Q000302:isolation & purification* / Q000378:metabolism; D017465:Receptors, Opioid, delta
D003432:Cross-Linking Reagents; D046911:Macromolecular Substances; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; C046222:3-methylfentanyl isothiocyanate; D005283:Fentanyl