Iodination of proteins on nitrocellulose blotting paper.
A procedure is described for radioiodination to a high specific activity of proteins immobilized on nitrocellulose membranes. After radioiodination, the proteins can be removed from the nitrocellulose to perform such structural analyses as proteolytic mapping. This method allows the detection of small amounts of protein recognized by immunoblotting and may be used to compare the structure of immunologically related proteins.
Hernández
M A
MA
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Padilla
R
R
Avila
J
J
D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D003101:Collodion; D000242:Cyclic AMP / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000032:analysis; D007553:Isotope Labeling / Q000379:methods*; D008868:Microtubule Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D014404:Tubulin / Q000378:metabolism
D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D014404:Tubulin; D003101:Collodion; D000242:Cyclic AMP; D011494:Protein Kinases