title: Ribosomal protein L2 in Saccharomyces cerevisiae is homologous to ribosomal protein L1 in Xenopus laevis. Isolation and characterization of the genes.
abstract: By cross-hybridization with a cDNA probe for the Xenopus laevis ribosomal protein L1 we have been able to isolate the homologous genes from a Saccharomyces cerevisiae genomic library. We have shown that these genes code for a ribosomal protein which was previously named L2. In yeast, like in X. laevis, these genes are present in two copies per haploid genome and, unlike the vertebrate counterpart, they do not contain introns. Amino acid comparison of the X. laevis L1 and S. cerevisiae L2 proteins has shown the presence of a highly conserved protein domain embedded in very divergent sequences. Although these sequences are very poorly homologous, they confer an overall secondary structure and folding highly conserved in the two species.
authors.affiliation: Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Roma, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Presutti
authors.forename: A
authors.initials: A
authors.lastname: Lucioli
authors.forename: I
authors.initials: I
authors.lastname: Bozzoni
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014982:Xenopus laevis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C028546:ribosomal protein L1; C029466:ribosomal protein L2; D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI