Developmental regulation and identification of an isotype encoded by altB, an alpha-tubulin locus in Physarum polycephalum.
A subcloned portion of the 5' nontranslated sequence from a Physarum alpha-tubulin cDNA is specific for a single alpha-tubulin locus, altB, of Physarum polycephalum. We find that this locus is expressed only in the plasmodium and encodes at least an alpha 1-tubulin isotype, which we have designated alpha 1B. Hybridization patterns of other subclones of this cDNA reveal two sequences for alpha-tubulin at the altB locus.
Green
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McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Schroeder
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Diggins
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Dove
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D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D008870:Microtubules / Q000502:physiology*; D010804:Physarum / Q000235:genetics* / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics; D008941:Spindle Apparatus / Q000502:physiology; D014404:Tubulin / Q000235:genetics*
D004271:DNA, Fungal; D012333:RNA, Messenger; D014404:Tubulin; D004262:DNA Restriction Enzymes