Identification and characterization of a Drosophila homologue to the vertebrate neuropeptide cholecystokinin.
Homologues to the cholecystokinin (CCK)-gastrin peptide family have been cloned from Drosophila. The CCK-like precursor found in Drosophila has been designated drosulfakinin (DSK). Genomic and cDNA clones corresponding to the Drosophila neuropeptide precursor encode for three putative peptides. The three peptides (DSK-0, Asn-Gln-Lys-Thr-Met-Ser-Phe-Gly; DSK-I, Phe-Asp-Asp-Tyr-Gly-His-Met-Arg-Phe-Gly; DSK-II, Gly-Gly-Asp-Asp-Gln-Phe-Asp-Asp-Tyr-Gly-His-Met-Arg-Phe-Gly) are flanked by prohormone processing sites and contain C-terminal glycyl residues, a potential amidation site. Two of the peptides, DSK-I and DSK-II, are homologous to CCK-gastrin peptides. Each of the two homologues include a CCK-gastrin-like C-terminal pentapeptide and a conserved sequence corresponding to the sulfated tyrosine in bioactive CCK. The third peptide encoded by the drosulfakinin precursor represents a novel peptide. In situ tissue hybridization indicates the presence of the transcript in the adult head. Chromosomal localization maps the gene to the third chromosome near 81F.
Nichols
R
R
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Schneuwly
S A
SA
Dixon
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002766:Cholecystokinin / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D015246:Deoxyribonuclease EcoRI; D004331:Drosophila melanogaster / Q000235:genetics*; D005755:Gastrins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009479:Neuropeptides; D009693:Nucleic Acid Hybridization; D009842:Oligopeptides / Q000235:genetics*; D010455:Peptides / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant; D005755:Gastrins; D036341:Intercellular Signaling Peptides and Proteins; D009479:Neuropeptides; D009842:Oligopeptides; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; C056935:drosulfakinin 0; C056936:drosulfakinin 1; C056938:drosulfakinin II; D004247:DNA; D002766:Cholecystokinin; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI