Bisphosphoglycerate mutase and pyruvate kinase activities during maturation of reticulocytes and ageing of erythrocytes.
An increase in bisphosphoglycerate mutase (BPGM) and a decrease in pyruvate kinase (PK), i.e. a decrease in PK/BPGM ratio, was observed in red cell populations from anemic rats containing 95% down to 3% reticulocytes in blood. Such a ratio has been used to study the fractionation of recticulocytes, according to their degree of maturation, after counter-current distribution of those cell populations in dextranpoly (ethylene glycol) two-phase systems. When applying this procedure to the fractionation according to age of erythrocytes from normal rats, the decrease of PK with cellular age was observed without a significant variation in BPGM activity.
Luque
J
J
Departmento de Bioquimica y Biologia Molecular, Universidad de Alcalã de Henares, Madrid, Spain.
Delgado
M D
MD
Rodriguez-Horche
P
P
Company
M T
MT
Pinilla
M
M
D000740:Anemia / Q000201:enzymology; D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000097:blood*; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000201:enzymology*; D004920:Erythropoiesis; D010770:Phosphotransferases / Q000097:blood*; D011770:Pyruvate Kinase / Q000097:blood*; D051381:Rats; D012156:Reticulocytes / Q000201:enzymology*
D010770:Phosphotransferases; D011770:Pyruvate Kinase; D001731:Bisphosphoglycerate Mutase