Quartz micropipettes for intracellular voltage microelectrodes and ion-selective microelectrodes.
We find that quartz is better than borosilicate glass as a material for micropipettes for certain microelectrode applications, including ion-selective microelectrodes of the liquid membrane type. Details are given of a graphite heating element that can be mounted on a standard microelectrode puller and used for making quartz micropipettes. A novel method of silanizing micropipettes is described. Experience with a novel miniature beveller that is fitted on a microscope stage is reported.
Munoz
J L
JL
Université de Genéve, Département d'oto-neuro-ophtalmologie, Switzerland.
Coles
J A
JA
D004594:Electrophysiology / Q000295:instrumentation; D008839:Microelectrodes; D009482:Neurophysiology / Q000295:instrumentation*; D011791:Quartz; D012822:Silicon Dioxide
D011791:Quartz; D012822:Silicon Dioxide