title: [Fluorescent properties of (Na+/K+)ATPase].
abstract: 1. The effect of ouabain on the molecular properties of (Na+/K+)-ATPase has been studied in purified preparations of the enzyme, isolated from the microsomal fraction of outer red medulla of porcine kidney, according to a modification of the method described by Jorgensen. 2. Ouabain, a specific inhibitor of (Na+/K+)-ATPase, binds at the potassium site of the enzyme, thus generating an increase in its stability towards the common denaturing agents, such as exposure to different concentration of guanidinium chloride (GdmC1) or to acidic solutions.
authors.affiliation: Istituto di Medicina Sperimentale CNR, Roma.
authors.forename: S
authors.initials: S
authors.lastname: Grimaldi
authors.forename: D
authors.initials: D
authors.lastname: Pozzi
authors.forename: E
authors.initials: E
authors.lastname: Pascale
authors.forename: M
authors.initials: M
authors.lastname: D'Onofrio
authors.forename: R
authors.initials: R
authors.lastname: Verna
authors.forename: G
authors.initials: G
authors.lastname: Giganti
authors.forename: F
authors.initials: F
authors.lastname: Monaco
authors.forename: J
authors.initials: J
authors.lastname: Roche
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005453:Fluorescence; D007668:Kidney / Q000648:ultrastructure; D008861:Microsomes / Q000201:enzymology; D010042:Ouabain / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013552:Swine
chemical_list: D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase
vernacular_title: Recherches sur les propriétés fluorescentes de la (Na+/K+) ATPase.