title: Effect of dietary selenium and vitamin E on the primary and secondary immune response in lambs challenged with parainfluenza3 virus.
abstract: This study was conducted to determine the effect of dietary selenium (Se) and vitamin E (Vit E) on various blood characteristics and the primary and secondary humoral immune response of lambs challenged with parainfluenza3 virus (PI3 V). Treatments included: 1) +Se/+Vit E, 2) -Se/+Vit E, 3) +Se/-Vit E and 4) -Se/-Vit E. The basal diet (-Se/-Vit E) was deficient in Se and Vit E. Sodium selenite (.2 mg Se/kg diet) and alpha-tocopherol acetate (20 mg Vit E/kg diet) were added to +Se and +Vit E diets, respectively, to provide adequate levels of each according to NRC recommendations. Following a 10-wk dietary adaptation and depletion period, lambs in all treatment groups were intratracheally inoculated with PI3 V on d 0 and 35 of the 70-d study. Prior to inoculation, whole blood and plasma glutathione peroxidase (GSH-Px) activities were higher (P less than .01) for +Se lambs. Whole blood and plasma GSH-Px increased (P less than .01) after primary viral inoculation in +Se lambs but not in -Se lambs. Serum immunoglobulin M (IgM) concentrations were enhanced (P less than .05) by Se supplementation on d 14, 35 and 49 of the study. Selenium and (or) Vit E did not affect serum immunoglobulin G (IgG) levels. Serum PI3 V antibody titers increased after inoculation on d 0 and 35 in all treatment groups. Titer levels appeared to increase more substantially for +Se lambs after primary inoculation, but increases were greater (P less than .01) for +Vit E lambs after secondary challenge.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Dept. of Anim. Sci., North Carolina State University, Raleigh 27695-7621.
authors.forename: J K
authors.initials: JK
authors.lastname: Reffett
authors.forename: J W
authors.initials: JW
authors.lastname: Spears
authors.forename: T T
authors.initials: TT
authors.lastname: Brown
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D003402:Creatine Kinase / Q000097:blood; D004032:Diet; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D010224:Parainfluenza Virus 3, Human / Q000276:immunology*; D018108:Respirovirus / Q000276:immunology*; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D012756:Sheep / Q000276:immunology*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014810:Vitamin E; D005979:Glutathione Peroxidase; D003402:Creatine Kinase; D012643:Selenium