title: Murine glia cells in culture can be stimulated to generate reactive oxygen.
abstract: Induction of luminol-enhanced chemiluminescence (CL) indicative of reactive oxygen formation was studied in glia cell cultures from newborn mice. A burst of CL could be induced by phorbol myristate acetate, zymosan, and antibody-coated bovine red blood cells, whereas Sendai virus and several other agents known to induce CL in myeloid cells were ineffective. Sodium azide failed to inhibit CL, indicating a myeloperoxidase-independent mechanism of light emission. In parallel experiments we identified the cells binding antibody-coated erythrocytes as macrophages characterized by the reduction of nitroblue tetrazolium and phagocytosis of zymosan and latex particles. Brain macrophages may use reactive oxygen intermediates (ROI) as a mechanism of antimicrobial defence; and, on the other hand, ROI formed by these cells may contribute to immuno-pathology in the brain.
authors.affiliation: Institute of Virology, University of Zürich, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Sonderer
authors.forename: P
authors.initials: P
authors.lastname: Wild
authors.forename: R
authors.initials: R
authors.lastname: Wyler
authors.forename: A
authors.initials: A
authors.lastname: Fontana
authors.forename: E
authors.initials: E
authors.lastname: Peterhans
authors.forename: M
authors.initials: M
authors.lastname: Schwyzer
mesh_terms: D000166:Acridines / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000906:Antibodies / Q000276:immunology; D001386:Azides / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008163:Luminescent Measurements; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009457:Neuroglia / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D009195:Peroxidase / Q000037:antagonists & inhibitors; D010587:Phagocytosis; D019810:Sodium Azide; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000166:Acridines; D000906:Antibodies; D001386:Azides; D007074:Immunoglobulin G; C033472:10,10'-dimethyl-9,9'-biacridinium; D009580:Nitroblue Tetrazolium; D015054:Zymosan; D019810:Sodium Azide; D009195:Peroxidase; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen