[Effect of cAMP and FSH on the production of estrogens by rat fetal gonads cultured in vitro].
The regulation of aromatase activity by cAMP and FSH has been demonstrated in the prepubertal rat testis and ovary, and the question posed whether this regulation was already operative at foetal stages. In the present study, testes and ovaries from 17- to 21-day-old rat foetuses were cultured in vitro in the presence of [3H]-testosterone and in the presence or absence of cAMP or FSH. Oestrone and oestradiol formed from [3H]-testosterone were measured by double isotopic dilution and recrystallization to constant specific activity. Aromatase activity was augmented in both gonads by cAMP, but only in the testis by FSH. Thus, the regulation of aromatase activity by FSH begins earlier in the testis than in the ovary.
Weniger
J P
JP
Laboratoire de Zoologie et d'Embryologie expérmentale de l'Université Louis-Pasteur, Strasbourg.
Zeis
A
A
D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D004958:Estradiol / Q000096:biosynthesis; D004967:Estrogens / Q000096:biosynthesis*; D004970:Estrone / Q000096:biosynthesis; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D010053:Ovary / Q000187:drug effects / Q000196:embryology* / Q000201:enzymology; D051381:Rats; D013737:Testis / Q000187:drug effects / Q000196:embryology* / Q000201:enzymology; D013739:Testosterone / Q000378:metabolism
D004967:Estrogens; D004970:Estrone; D013739:Testosterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D001141:Aromatase
Action de l'AMPc et de FSH sur la production d'oestrogènes par les gonades foetales de rat en culture in vitro.