Coordinate expression of the endogenous p53 gene in beta cells of transgenic mice expressing hybrid insulin-SV40 T antigen genes.
The expression of p53 has been evaluated during oncogenesis of the pancreatic beta cells in transgenic mice harboring hybrid insulin-SV40 T antigen genes. Significant levels of p53 are detected in all cells expressing large T antigen. In contrast, the protein is undetectable in normal beta cells. There is a complete correspondence between the onset of expression of T antigen and the appearance of the endogenous p53 protein. In tumors, the two proteins are found in a complex. In addition, free uncomplexed T antigen is detected in every cell which expresses the transgene. These results are consistent with the participation of p53 in T antigen-induced tumorigenesis in vivo. The early appearance of p53 in all beta cells expressing large T cannot readily explain the progression of a small fraction of these cells into solid tumors.
Efrat
S
S
Cold Spring Harbor Laboratory, NY 11724.
Baekkeskov
S
S
Lane
D
D
Hanahan
D
D
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D005796:Genes; D005814:Genes, Viral; D007328:Insulin / Q000235:genetics*; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000235:genetics*; D009698:Nucleoproteins / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000382:microbiology; D010750:Phosphoproteins / Q000235:genetics*; D011230:Precancerous Conditions / Q000235:genetics / Q000382:microbiology; D055503:Protein Multimerization; D013539:Simian virus 40 / Q000235:genetics*; D016159:Tumor Suppressor Protein p53
D000952:Antigens, Polyomavirus Transforming; D007328:Insulin; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53