Presence of functional receptors for atrial natriuretic peptide in human pheochromocytoma.
Pheochromocytoma, a catecholamine-secreting adrenomedullary tumor, has been shown to contain the functional receptor for human atrial natriuretic peptide(h-ANP). Release of catecholamines from tissue slices of pheochromocytoma was inhibited by h-ANP in a dose-dependent manner. Binding assays using 125I-ANP revealed a single class of high affinity binding sites for ANP. When covalently tagged with 125I-ANP and electrophoresed under non-reducing and reducing conditions, the receptor migrated as a 140-kDa band and a 70-kDa band, respectively, reflecting its disulfide-linked subunit structure. The presence of ANP receptor in pheochromocytoma was further demonstrated by immunohistochemistry; the tumor was positively stained with an antireceptor antiserum. The antiserum was also useful to establish the zona glomerulosa localization of ANP receptor in the normal human adrenal gland.
Shionoiri
H
H
Second Department of Internal Medicine, Yokohama City University, Japan.
Hirawa
N
N
Takasaki
I
I
Ishikawa
Y
Y
Minamisawa
K
K
Miyajima
E
E
Kinoshita
Y
Y
Shimoyama
K
K
Shimonaka
M
M
Ishido
M
M
D000310:Adrenal Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology; D004837:Epinephrine / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009638:Norepinephrine / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism* / Q000473:pathology; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*
D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D009638:Norepinephrine; D004837:Epinephrine