title: Demonstration of human papillomavirus types 6 and 11 in juvenile laryngeal papillomatosis by in-situ DNA hybridization.
abstract: A study is reported in which an in situ hybridization technique for the demonstration of human papillomavirus (HPV) employing a biotin--streptavidin polyalkaline phosphatase complex has been successfully applied to formalin-fixed paraffin-processed tissue obtained from ten patients with juvenile laryngeal papillomatosis. In all cases, positive results were obtained for either HPV type 6 or 11. Normal vocal cord epithelium was negative.
authors.affiliation: Department of ENT Surgery, Bradford Royal Infirmary, U.K.
authors.forename: R M
authors.initials: RM
authors.lastname: Terry
authors.forename: F A
authors.initials: FA
authors.lastname: Lewis
authors.forename: S
authors.initials: S
authors.lastname: Griffiths
authors.forename: M
authors.initials: M
authors.lastname: Wells
authors.forename: C C
authors.initials: CC
authors.lastname: Bird
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007822:Laryngeal Neoplasms / Q000382:microbiology*; D008828:Microbiological Techniques; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000382:microbiology*; D027383:Papillomaviridae / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral