A preparative method for the enzymatic 5'-monophosphorylation of nucleosides.
A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or [gamma-32P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed.
Merkler
D J
DJ
Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Schramm
V L
VL
D000248:Adenosine Kinase; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D008099:Liver / Q000201:enzymology; D009705:Nucleosides / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases
D009705:Nucleosides; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D000248:Adenosine Kinase