The duck beta-globin gene cluster contains a single enhancer element.
An erythroid-specific enhancer was previously identified in the 3'-flanking region of the beta adult gene in chicken and duck, by transfection into AEV transformed chicken erythroblasts. Here we show that the duck enhancer is equally active in erythroid human K562 cells, presenting an embryonic/fetal program of globin gene expression. Furthermore, no other enhancer was found within the 20 kb of DNA including four beta-like globin genes as well as a 1.5 kb upstream and a 3 kb downstream sequence.
Kretsovali
A
A
Institut Jacques Monod, Paris, France.
Marcaud
L
L
Huesca
M
M
Scherrer
K
K
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004372:Ducks; D004742:Enhancer Elements, Genetic; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D014162:Transfection
D005914:Globins; D004262:DNA Restriction Enzymes