title: Intraplasmid recombination in Streptomyces lividans 66.
abstract: An Escherichia coli-Streptomyces shuttle plasmid pIF132 containing two direct mel repeats was constructed. While pIF132 replicated relatively stably in E. coli (Rec+ or recA), its structure was unstable in S. lividans: recombination between the mel repeats resulted in a smaller plasmid, pIF138. Furthermore, pIF132 formed oligomers extensively in E. coli but not in S. lividans.
authors.affiliation: Institute of Microbiology and Immunology, National Yangming Medical College, Taipei, Taiwan, Republic of China.
authors.forename: C W
authors.initials: CW
authors.lastname: Chen
authors.forename: J F
authors.initials: JF
authors.lastname: Tsai
authors.forename: S E
authors.initials: SE
authors.lastname: Chuang
mesh_terms: D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000302:isolation & purification; D005822:Genetic Vectors; D010957:Plasmids; D011995:Recombination, Genetic; D013302:Streptomyces / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes