title: Carrageenin-stimulated peritoneal macrophages release in vitro collagenase and gelatinase.
abstract: Guinea pig macrophages were obtained by intraperitoneal injection of carrageenin, and in contrast with peritoneal cells from non-stimulated animals, release in vitro an active collagenase into the culture medium. Short time trypsin incubations and treatment of enzyme preparations with 4-APMA failed to reveal latent enzyme activity. Metallopeptidases specific for gelatin paralleled the presence of collagenase in the media of stimulated cells. Raw medium was fractionated by affinity chromatography with heparin-sepharose, collagenase activity bound to heparin sepharose while a gelatinase with an apparent molecular weight of 54,800 did not. When macrophages were grown in vitro in the presence of albumin, the gelatinase activity showed affinity for heparin and an apparent molecular weight of 113,000.
authors.affiliation: Departamento de Biologia, Facultad de Ciencias, Universidad Nacional Autónoma de México.
authors.forename: R
authors.initials: R
authors.lastname: López-Escalera
authors.forename: A
authors.initials: A
authors.lastname: Pardo
mesh_terms: D000818:Animals; D002351:Carrageenan / Q000494:pharmacology*; D002478:Cells, Cultured; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D018093:Gelatinases; D006168:Guinea Pigs; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D003012:Microbial Collagenase / Q000378:metabolism*; D008970:Molecular Weight; D010434:Pepsin A / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology*
chemical_list: D002351:Carrageenan; D010434:Pepsin A; D018093:Gelatinases; D003012:Microbial Collagenase