Bovine seminalplasmin [corrected] is a DNA unwinding protein.
The duplex DNA unwinding ability of seminalplasmin [corrected] from bovine semen was examined by treatment of plasmid-protein complexes with calf thymus topoisomerase I and resolution of the topoisomer distributions by agarose gel electrophoresis. Binding of seminalplasmin [corrected] results in a moderate degree of unwinding of supercoiled plasmid. The elongation of the RNA chain by E. coli RNA polymerase over promoter containing template is not inhibited by seminalplasmin [corrected]. However, the reinitiation of transcription is blocked in such cases indicating that seminalplasmin [corrected] inhibits transcription by binding to the initiation site of RNA polymerase.
Gopal
V
V
Centre for Cellular and Molecular Biology, Hyderabad, India.
Kumar
K P
KP
Chatterji
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D
D000818:Animals; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors; D002417:Cattle; D004265:DNA Helicases / Q000378:metabolism* / Q000494:pharmacology; D004269:DNA, Bacterial / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005341:Fibrinolysin / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D010957:Plasmids; D012318:RNA Polymerase I / Q000037:antagonists & inhibitors; D014158:Transcription, Genetic / Q000187:drug effects*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D012318:RNA Polymerase I; D005341:Fibrinolysin; D004265:DNA Helicases