Effect of lipid hydroperoxide on Xenopus oocytes and on neurotransmitter receptors synthesized in Xenopus oocytes injected with exogenous mRNA.
The effect of 13-L-hydroperoxylinoleic acid (LOOH) on both Xenopus oocytes and neurotransmitter receptors synthesized in the oocytes was studied by electrophysiological and ion flux measurement. Addition of LOOH to the incubation mixture of the oocytes raised the membrane potential and decreased the membrane resistance of the oocytes. These effects of LOOH on the oocytes were reversed within a few hours by incubation with frog Ringer solution. Addition of LOOH also caused an increase of Li+ and 45Ca2+ uptake into the oocytes. However, production of alkoxy radicals by the addition of FeCl2 to the incubation mixture containing LOOH did not accelerate the damage to the oocytes by LOOH. So essential toxicity is caused possibly by an increase in the membrane permeability resulting from disturbance of the lipid bilayer arrangement, not from production of active alkoxy radicals during decomposition of LOOH. Nicotinic acetylcholine and gamma-aminobutyric acid receptors were synthesized in Xenopus oocytes by injecting mRNA prepared from Electrophorus electricus electroplax and rat brain. LOOH noncompetitively inhibited the function of these receptors and also increased the rate of desensitization of the receptors.
Aoshima
H
H
Department of Chemistry, Faculty of Liberal Arts, Yamaguchi University, Japan.
Anan
M
M
Ishii
H
H
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004553:Electric Conductivity; D008041:Linoleic Acids / Q000494:pharmacology*; D008054:Lipid Peroxides; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D011950:Receptors, Cholinergic / Q000096:biosynthesis* / Q000187:drug effects; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000187:drug effects; D014981:Xenopus
D008041:Linoleic Acids; D008054:Lipid Peroxides; D011950:Receptors, Cholinergic; D011963:Receptors, GABA-A; C032890:13-hydroperoxylinoleic acid; D002118:Calcium