title: Identification of the structural gene encoding the SH-activated hemolysin of Listeria monocytogenes: listeriolysin O is homologous to streptolysin O and pneumolysin.
abstract: By immunoblotting with an antiserum raised against purified listeriolysin O, we have detected the presence of a truncated protein of 52 kilodaltons in culture supernatants of a Tn1545-induced nonhemolytic mutant of Listeria monocytogenes (J.L. Gaillard, P. Berche, and P. Sansonetti, Infect. Immun. 52:50-55, 1986). The region of insertion of the transposon has been cloned and sequenced. The transposon had inserted in an open reading frame the listeriolysin O gene. The deduced amino acid sequence of this open reading frame revealed that listeriolysin O is homologous to streptolysin O and pneumolysin, although homologies were not detectable at the DNA level.
authors.affiliation: Unité de Génie Microbiologique, Institut Pasteur, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Mengaud
authors.forename: J
authors.initials: J
authors.lastname: Chenevert
authors.forename: C
authors.initials: C
authors.lastname: Geoffroy
authors.forename: J L
authors.initials: JL
authors.lastname: Gaillard
authors.forename: P
authors.initials: P
authors.lastname: Cossart
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D008089:Listeria monocytogenes / Q000235:genetics* / Q000472:pathogenicity; D008970:Molecular Weight
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes