The pentose phosphate pathway in the endoplasmic reticulum.
Approximately the same levels of six of the seven enzymes catalyzing reactions of the pentose phosphate pathway are in the cisternae of washed microsomes from rat heart, spleen, lung, and brain. Renal and hepatic microsomes also have detectable levels of these enzymes except ribulose-5-phosphate epimerase and ribose-5-phosphate isomerase. Their location in the cisternae is indicated by their latencies, i.e. requirement for disruption of the membrane for activity. In addition, transketolase, transaldolase, and glucose-6-phosphatase, a known cisternal enzyme, are inactivated by chymotrypsin and subtilisin only in disrupted hepatic microsomes under conditions in which NADPH-cytochrome c reductase, an enzyme on the external surface, is inactivated equally in intact and disrupted microsomes. The failure to detect the epimerase and isomerase in hepatic microsomes is due to inhibition of their assays by ketopentose-5-phosphatase. Xylulose 5-phosphate is hydrolyzed faster than ribulose 5-phosphate. A mild heat treatment destroys hepatic xylulose-5-phosphatase and glucose-6-phosphatase without affecting acid phosphatase. These results plus the established wide distribution of glucose dehydrogenase, the microsomal glucose-6-phosphate dehydrogenase, and its localization to the lumen of the endoplasmic reticulum suggest that most mammalian cells have two sets of enzymes of the pentose phosphate pathway: one is cytoplasmic and the other is in the endoplasmic reticulum. The activity of the microsomal pentose phosphate pathway is estimated to be about 1.5% that of the cytoplasmic pathway.
Bublitz
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Department of Biochemistry, Biophysics, and Genetics, University of Colorado Health Sciences Center, Denver 80262.
Steavenson
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D000818:Animals; D002238:Carbohydrate Epimerases / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010427:Pentose Phosphate Pathway; D051381:Rats; D014153:Transaldolase / Q000378:metabolism; D014174:Transketolase / Q000378:metabolism
D009251:NADPH-Ferrihemoprotein Reductase; D014174:Transketolase; D014153:Transaldolase; D005952:Glucose-6-Phosphatase; D002238:Carbohydrate Epimerases; C022554:L-ribulosephosphate 4-epimerase; C023258:UDPglucuronate 4-epimerase