Effects of lysophosphatidylcholine and palmitylcarnitine--lipid metabolites produced in ischemia--on porcine coronary and rabbit femoral arteries.
In organ bath experiments, amphiphilic lipids lysophosphatidylcholine (LPC) and palmitylcarnitine (PLC) produced a small increase in tension of nonprecontracted strips of porcine coronary artery with a subsequent decrease to initial level after high concentrations of the agents, both in intact and endothelium-denuded preparations. Both amphiphiles produced dose-dependent but incomplete relaxation of intact coronary strips precontracted with high potassium. The effect of PLC was more pronounced. LPC, 3.10(-6) mol.l-1, did not influence Ca++-dose-response relationships, while PLC in concentration of 10(-5) mol.l-1 abolished the decline in the second Ca++-dose-response curve. Neither PLC nor LPC in concentrations of 3.10(-6) mol.l-1 influenced endothelium-dependent relaxation produced by bradykinin precontracted with high potassium porcine coronary artery. Both amphiphiles did not change tension of nonprecontracted and precontracted with phenylephrine, 10(-6) mol.l-1, rabbit femoral artery ring segments or Ca++-dose-response relationships with and without endothelium.
Vedernikov YuP
Department of Human Cardiovascular Pathology, All-Union Cardiology Research Centre, Academy of Medical Sciences, Moscow, USSR.
Gräser
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Tiedt
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Vikhert
A M
AM
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002331:Carnitine / Q000031:analogs & derivatives*; D003331:Coronary Vessels / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000378:metabolism; D005263:Femoral Artery / Q000187:drug effects*; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D010172:Palmitoylcarnitine / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011817:Rabbits; D013552:Swine
D008244:Lysophosphatidylcholines; D010172:Palmitoylcarnitine; D010656:Phenylephrine; D011188:Potassium; D002331:Carnitine; D001920:Bradykinin; D002118:Calcium