Method for rapid isolation and analysis of algal virus DNA.
A quick method has been developed for isolating viral DNA from small cultures of eukaryotic algae infected with PBCV-1 or similar viruses. The DNA preparations are virtually free of contaminating host DNA and are suitable substrates for restriction enzymes. 5 ml cultures yield 8-25 micrograms of viral DNA. Many plaque-purified isolates from environmental water samples can be analyzed in a day.
Howarth
A J
AJ
Department of Plant Pathology, University of Arizona, Tucson 85721.
D002708:Chlorella; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification*; D008828:Microbiological Techniques; D010942:Plant Viruses / Q000302:isolation & purification*; D010948:Viral Plaque Assay; D014871:Water Microbiology
D004279:DNA, Viral; D004262:DNA Restriction Enzymes