Kinetics of pump currents generated by the Na+,K+-ATPase.
Purified Na+,K+-ATPase from pig kidney was attached to black lipid membranes. Pump currents of the enzyme could be measured with a time resolution of approx. 1 ms by releasing ATP from caged ATP with a UV laser flash. Analysis of the transient currents shows that a slow non-electrogenic step is followed by an electrogenic transition with a rate constant of 100 s-1 (22 degrees C). The exponential components found in the transient currents are compared to transitions in the Albers-Post scheme.
Fendler
K
K
Max-Planck-Institut für Biophysik, Frankfurt am Main, FRG.
Grell
E
E
Bamberg
E
E
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008051:Lipid Bilayers / Q000528:radiation effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013552:Swine; D014466:Ultraviolet Rays
D008051:Lipid Bilayers; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase