Serum albumin treatment to eliminate false-positive results with laboratory rodent specimens tested by rotazyme enzyme-linked immunosorbent assay.
A high proportion of positive results was found in fecal specimens from mice and rats tested by Rotazyme II enzyme-linked immunosorbent assay, but the presence of virus could not be confirmed. Positive specimens and nonautoclaved rodent diet contained a substance that apparently attached nonspecifically to the antibody-coated beads used in the test and reacted directly with the substrate. Pretreatment of the beads with 0.1% bovine serum albumin eliminated this nonspecific activity.
Jure
M N
MN
Laboratory Animal Research Center, Rockefeller University, New York, New York 10021-6399.
Morse
S S
SS
Stark
D M
DM
D000821:Animal Feed; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005243:Feces / Q000382:microbiology; D051379:Mice; D008863:Microspheres; D051381:Rats; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis*; D012710:Serum Albumin, Bovine
D012710:Serum Albumin, Bovine