Variable expression of SV40 large T antigen in CV1 cell clones.
Using immunofluorescence and immunoadsorption, CV1 cell clones MA2, V4, USA3, TR7 and P3 infected with SV40 were found to express variably SV40 large T antigen. The monoclonal antibody used was Pab 419. The results indicate that P3 cells express T antigen to a considerable level as early as 10 h post-infection, while that of TR7 and USA3 cells is minute as judged from their positive nuclei. MA2 and V4 cells did not show any positive nuclei over this period of infection. At 20 h post-infection MA2, V4 and USA3 cells developed a considerable amount of fluorescence in their nuclei while TR7 and P3 cells produced high values. By immunoadsorption of cell extracts for the same periods of infection, similar results were obtained on the electrophoretograms. We also relate these findings with those from induction of heatshock proteins by SV40 infection.
Matthopoulos
D P
DP
Laboratory of General Biology, Medical School, University of Ioannina, Greece.
Pagoulatos
G N
GN
D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis*; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D002999:Clone Cells / Q000276:immunology / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D007163:Immunosorbent Techniques; D013539:Simian virus 40 / Q000276:immunology / Q000502:physiology
D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming