[The effect of biomaterials on cytochrome c].
The adsorption of ferricytochrome c and ferrocytochrome c on biomaterials was studied by measuring the spectrum of samples assuming the molar extinction coefficients of the ferricytochrome c and ferrocytochrome c at 480 nm and 550 nm. Silica, alumina ceramic, zirconium ceramic, tricalcium phosphate ceramic, hydroxyapatite ceramic, unidirectional calcium phosphate glass-ceramic, bulk crystallized calcium phosphate glass-ceramic and bulk crystallized calcium phosphate glass-ceramic containing large amount of titanium were used as adsorbents. The adsorption of ferricytochrome c or ferrocytochrome c on silica essentially followed the Langumuir adsorption isotherm. Quantitative analysis showed that ferricytochrome c was adsorbed more and dissolved less than ferrocytochrome c on batch adsorption on silica. Total amounts of adsorbed and non-adsorbed ferricytochrome c increased significantly in batch adsorption of ferrocytochrome c on silica. From these experiments it appears that materials such as silica induce a transfer of electrons of cytochrome c.
Nagase
M
M
Department of Orthopaedic Surgery, Gunma University School of Medicine, Japan.
Chigira
M
M
Shimizu
T
T
Shinozaki
T
T
Udagawa
E
E
D000327:Adsorption; D001672:Biocompatible Materials / Q000494:pharmacology*; D002516:Ceramics / Q000494:pharmacology; D003574:Cytochrome c Group / Q000032:analysis / Q000378:metabolism*; D012822:Silicon Dioxide / Q000494:pharmacology
D001672:Biocompatible Materials; D003574:Cytochrome c Group; D012822:Silicon Dioxide