Cytochromes P-450 of sea birds: cross-reactivity studies with purified rat cytochromes.
1. Polyclonal antibodies against rat cytochrome P-450c (IA1), P-450d (IA2), P-450b (IIB1), P-450h (IIC11) and P-450j (IIE1), were used to probe liver microsomes prepared from six sea bird species collected from the Irish Sea between 1978 and 1988. 2. Significant cross-reactivity in all the sea bird species was seen only with antibodies to P450 IA1. Expression of cross-reactivity proteins was highly variable between individual birds, which show evidence of environmental induction. 3. Shared epitopes to P450 IA1 and IA2 were seen on a single protein expressed in liver microsomes from the cormorant (Phalacrocorax carbo). 4. Antibodies against members of rat P450 gene family II showed a small degree of cross-reactivity with sea bird microsomes. Antibodies against P450 IIB1 and IIC11 showed weak cross-reactivity in all species with little inter-individual variation. Antibodies to P450 IIE1 showed no cross-reactivity in any bird species. 5. P450 gene family I appears to be well represented in sea birds while P450 gene family II is not well developed in this group of lower vertebrates.
Ronis
M J
MJ
Department of Physiological Chemistry, Karolinska Institute, Stockholm, Sweden.
Hansson
T
T
Borlakoglu
J
J
Walker
C H
CH
D000818:Animals; D000906:Antibodies; D001717:Birds / Q000235:genetics*; D015153:Blotting, Western; D003429:Cross Reactions; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051381:Rats / Q000235:genetics*; D013045:Species Specificity
D000906:Antibodies; D003577:Cytochrome P-450 Enzyme System