Change in the amount of rRNA precursor synthesized in rat ventral prostates after administration of androgen and cycloheximide: application of quantitative S1 nuclease mapping.
The change in amount of pre-rRNA was observed by the use of total cellular RNA isolated from rat ventral prostates and quantitative S1 nuclease mapping. The amount of pre-rRNA from castrated rats was about 20% of that of normal rats, and increased to the normal level 24h after administration of testosterone. Cycloheximide caused a rapid decrease in the amount of pre-rRNA in normal and testosterone-treated castrated rats, to about 20% and 10% of that of normal rats, respectively, 2h after the administration of the drug. These results support the view that the androgen-stimulated initiation of the rRNA gene transcription is mediated by a hormone-dependent short-lived protein(s).
Matsui
H
H
Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Horikawa
S
S
Suzuki
N
N
Tsukada
K
K
Hosoya
T
T
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D004720:Endonucleases; D005796:Genes / Q000187:drug effects; D007700:Kinetics; D008297:Male; D009710:Nucleotide Mapping; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D012322:RNA Precursors / Q000187:drug effects / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013739:Testosterone / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D012322:RNA Precursors; D013739:Testosterone; D003513:Cycloheximide; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases