title: Localization of high-affinity and low-affinity nerve growth factor receptors in cultured rat basal forebrain.
abstract: Previous work has indicated that nerve growth factor specifically and selectively increases choline acetyltransferase and acetylcholinesterase in organotypic cultures of rat basal forebrain-medial septal area. To determine whether these actions are potentially receptor-mediated, organotypic and dissociated basal forebrain-medial septal area cultures were examined. Two independent methods, [125I]nerve growth factor binding and immunocytochemistry with a monoclonal nerve growth factor receptor antibody (192-IgG), detected specific receptors. The nerve growth factor receptors were localized to two different cellular populations: flat, large, non-neuron-like cells, and small, round, process-bearing, neuron-like cells. Dissociation studies with [125I]nerve growth factor suggested that high-affinity receptors were localized to the neuron-like population, while only low-affinity receptors were localized to the non-neuron-like cells. We tentatively conclude that nerve growth factor may elicit cholinergic effects by directly binding to high-affinity receptors on neurons. To begin examining receptor regulation, cultures were exposed to exogenous, unlabeled nerve growth factor continuously for 10 days before binding studies were performed. Prior exposure to nerve growth factor did not alter binding characteristics of the receptor, using the present methods.
authors.affiliation: Department of Anatomy, Mount Sinai School of Medicine, New York, NY 10029.
authors.forename: P
authors.initials: P
authors.lastname: Bernd
authors.forename: H J
authors.initials: HJ
authors.lastname: Martinez
authors.forename: C F
authors.initials: CF
authors.lastname: Dreyfus
authors.forename: I B
authors.initials: IB
authors.lastname: Black
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D009414:Nerve Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor; D012686:Septal Nuclei / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor