The interaction between the deacetylated (amine) metabolite of diamphenethide (DAMD) and cytochemically demonstrable Na+/K+-ATPase activity in the tegument of Fasciola hepatica.
The relative effects on tegumental Na+/K+-ATPase activity in Fasciola hepatica of the deacetylated (amine) metabolite of diamphenethide (DAMD) (10 micrograms/ml, 18 h) and the Na+/K+-ATPase inhibitor ouabain (0.1 mM, 0.5 h) have been assessed cytochemically. In the normal tegument, Na+/K+-ATPase activity is particularly concentrated along the invaginations of the apical plasma membrane and the infoldings of the basal plasma membrane. Ouabain pretreatment significantly reduces the overall level of Na+/K+-ATPase activity, but does not induce swelling of the basal infolds. In contrast, DAMD does not significantly reduce either the level or distribution of Na+/K+-ATPase activity, but does cause a pronounced swelling of the basal infolds. The results are discussed in relation to the postulated action of diamphenethide as an inhibitor of Na+/K+-ATPase activity.
Skuce
P J
PJ
Department of Zoology, Queen's University, Belfast, Northern Ireland.
Anderson
H R
HR
Fairweather
I
I
D000083:Acetanilides / Q000494:pharmacology*; D000818:Animals; D003957:Diamfenetide / Q000378:metabolism / Q000494:pharmacology*; D005210:Fasciola hepatica / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D006651:Histocytochemistry; D008854:Microscopy, Electron; D010042:Ouabain / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000083:Acetanilides; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D003957:Diamfenetide