title: Heterogeneity of genome rearrangements in rotaviruses isolated from a chronically infected immunodeficient child.
abstract: Rotaviruses with genome rearrangements, isolated from a chronically infected immunodeficient child, were adapted to growth in BSC-1 cells. Preparations of viral RNA from fecal extracts showed a mixed atypical rotavirus RNA profile, which was due to the presence of at least 12 subpopulations of viruses grossly differing in genotype. Besides various forms of genome rearrangements involving segment 8-, 10-, and 11-specific sequences, reassortment in vivo was likely to have occurred during the emergence of these viruses. The protein products of viral genomes with various forms of segmental rearrangements seemed to be largely unaltered. Genome rearrangement is proposed to be a third mechanism directing the evolution of rotaviruses.
authors.affiliation: Institute of Virology, University of Glasgow, United Kingdom.
authors.forename: F
authors.initials: F
authors.lastname: Hundley
authors.forename: M
authors.initials: M
authors.lastname: McIntyre
authors.forename: B
authors.initials: B
authors.lastname: Clark
authors.forename: G
authors.initials: G
authors.lastname: Beards
authors.forename: D
authors.initials: D
authors.lastname: Wood
authors.forename: I
authors.initials: I
authors.lastname: Chrystie
authors.forename: U
authors.initials: U
authors.lastname: Desselberger
mesh_terms: D000818:Animals; D002460:Cell Line; D002648:Child; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000382:microbiology*; D008854:Microscopy, Electron; D008970:Molecular Weight; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral