Isolation and characterization of thermosensitive mutants from Kilham rat virus, a rodent parvovirus.
Thermosensitive mutants were isolated from nitrous acid-treated Kilham rat virus (KRV). At a restrictive temperature (39.5 degrees C), the mutants tested did not produce appreciable amounts of infectious particles, haemagglutinin or progeny single-stranded DNA. Virus antigen accumulation as detected by immunoperoxidase was reduced. Complementation tests revealed two distinct groups. The three members of complementation group 1 synthesized normal amounts of replicative forms but were restricted in single-stranded DNA production and capsid protein expression, exhibiting phenotypes compatible with cap mutations. The sole representative of group 2, KRV ts6, failed to accumulate replicative forms, displaying a rep- phenotype. These mutants provide new tools to test the role of viral products in the biology of autonomous parvoviruses.
Wicker
R
R
Institut Curie, Section de Biologie, Paris, France.
Gunther
M
M
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002213:Capsid / Q000032:analysis; D004279:DNA, Viral / Q000096:biosynthesis; D005814:Genes, Viral; D005816:Genetic Complementation Test; D006358:Hot Temperature; D007124:Immunoenzyme Techniques; D007700:Kinetics; D009154:Mutation; D009608:Nitrous Acid / Q000494:pharmacology; D009698:Nucleoproteins / Q000032:analysis; D010321:Parvoviridae / Q000302:isolation & purification*; D017992:Parvovirus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010641:Phenotype; D051381:Rats; D011995:Recombination, Genetic; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral; D009698:Nucleoproteins; D009608:Nitrous Acid