Sequence of cDNAs encoding subunit Vb of human and bovine cytochrome c oxidase.
We have isolated a full-length human fetal muscle cDNA clone specifying the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme, cytochrome c oxidase (COX; EC 1.9.3.1), and a partial-length brain cDNA clone specifying the analogous bovine subunit. The two cDNAs are 85% identical at the nucleotide level. Similar to other proteins imported into mitochondria, the deduced human COX Vb protein contains a presequence, 31 amino acids long, rich in basic residues. We find no evidence for tissue-specific transcripts for subunit Vb of human COX, as Northern analysis of total RNA from human muscle, liver, and brain showed a single, identically sized transcript in each cell type, while partial-length cDNA clones isolated from human muscle and endothelial cell cDNA libraries were identical in sequence to the fetal muscle cDNA.
Zeviani
M
M
H. Houston Merritt Clinical Research Center for Muscular Dystrophy and Related Disorders, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Sakoda
S
S
Sherbany
A A
AA
Nakase
H
H
Rizzuto
R
R
Samitt
C E
CE
DiMauro
S
S
Schon
E A
EA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D003576:Electron Transport Complex IV / Q000235:genetics*; D005796:Genes; D006801:Humans; D046911:Macromolecular Substances; D008929:Mitochondria, Heart / Q000201:enzymology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D013045:Species Specificity
D046911:Macromolecular Substances; D004247:DNA; D003576:Electron Transport Complex IV; D004262:DNA Restriction Enzymes