Characterization of the mouse met proto-oncogene.
The DNA sequence of cDNA clones prepared from transcripts of the mouse met proto-oncogene reveals that the mouse met gene encodes a 1380 amino acid protein with the characteristics of a growth factor receptor. This protein can be divided into several putative domains, including an intracellular protein tyrosine kinase domain, a transmembrane domain and a 929 amino acid extracellular domain, possessing a potential proteolytic cleavage site with the sequence Lys-Arg-Arg-Lys-Arg-Ser. To gain additional insights into the function of the met protein we have examined the level of met transcripts in tissues of the late-gestation mouse conceptus. Transcription of met was observed in most of the tissues analysed, but the highest levels of met mRNA were detected in the yolk sac, amnion and kidney; no transcripts were detectable in the calvaria. Chromosomal localization using a series of mouse-hamster hybrid cell lines has demonstrated that met is located on mouse chromosome 6.
Chan
A M
AM
Chemical Carcinogenesis Section, Chester Beatty Laboratories, London, UK.
King
H W
HW
Deakin
E A
EA
Tempest
P R
PR
Hilkens
J
J
Kroezen
V
V
Edwards
D R
DR
Wills
A J
AJ
Brookes
P
P
Cooper
C S
CS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006133:Growth Substances; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D011956:Receptors, Cell Surface / Q000235:genetics*
D006133:Growth Substances; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface