title: Rapid assessment of relationships among HIV isolates by oligopeptide analyses of external envelope glycoproteins.
abstract: The most variable proteins, the gp120's, of the many isolates of HIV-I can be readily compared by two-dimensional oligopeptide maps. The gp120 in a given cell line is completely stable, but the cell line defines the actual gp120 size and may induce minor peptide changes. HTLV-IIIB and LAV differ slightly from each other even when grown in the same cell line, while LAV grown in a B cell line is less related. Molecularly distant isolates have unique patterns. While anti-HTLV-IIIB gp120 antibody neutralized both HTLV-IIIB and LAV, it recognizes only the homologous HTLV-IIIB infected cells in cytotoxicity assays. Structural analysis of isolates should be helpful in defining the range of immunological reactivities among variants as a contribution to a rational approach to a vaccine against AIDS.
authors.affiliation: Abbott Laboratories, Abbott Park, IL 60064.
authors.forename: W G
authors.initials: WG
authors.lastname: Robey
authors.forename: P L
authors.initials: PL
authors.lastname: Nara
authors.forename: C M
authors.initials: CM
authors.lastname: Poore
authors.forename: M
authors.initials: M
authors.lastname: Popovic
authors.forename: M F
authors.initials: MF
authors.lastname: McLane
authors.forename: F
authors.initials: F
authors.lastname: Barin
authors.forename: M
authors.initials: M
authors.lastname: Essex
authors.forename: P J
authors.initials: PJ
authors.lastname: Fischinger
mesh_terms: D002460:Cell Line; D002846:Chromatography, Affinity; D002918:Chymotrypsin; D004591:Electrophoresis, Polyacrylamide Gel; D006678:HIV / Q000235:genetics / Q000302:isolation & purification*; D015699:HIV Envelope Protein gp120; D006801:Humans; D009842:Oligopeptides / Q000032:analysis; D012191:Retroviridae Proteins / Q000235:genetics / Q000302:isolation & purification*; D014759:Viral Envelope Proteins / Q000302:isolation & purification*
chemical_list: D015699:HIV Envelope Protein gp120; D009842:Oligopeptides; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins; D002918:Chymotrypsin