Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus.
A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5' termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.
Keen
C L
CL
Department of Microbiology, University of Alberta, Edmonton, Canada.
Mendelovitz
S
S
Cohen
G
G
Aharonowitz
Y
Y
Roy
K L
KL
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes