Autoradiographic localization of gamma-aminobutyric acid receptors in mouse barrel field.
The distribution of high-affinity gamma-aminobutyric acid (GABA) receptors in mouse posteromedial barrel subfield (PMBSF) in layer IV of the somatosensory (SI) cortex was studied using [3H]muscimol autoradiography in vitro. A qualitative study revealed a heterogeneous distribution in the density of [3H]muscimol binding in the barrel field. In the barrel sides and septum between the barrels [3H]muscimol binding exhibits the lowest level of labelling in the entire tissue. In comparison, [3H]muscimol binding in the hollows is considerably higher than in the barrel sides although the level is not homogeneous. These findings suggest that the barrel hollows are much richer in GABA receptors than the surrounding barrel sides and septa.
Chmielowska
J
J
Brain Research Group, Open University, Milton Keynes, U.K.
Stewart
M G
MG
Bourne
R C
RC
D000818:Animals; D001345:Autoradiography; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009118:Muscimol; D011963:Receptors, GABA-A / Q000378:metabolism*; D013003:Somatosensory Cortex / Q000378:metabolism*; D014738:Vibrissae / Q000502:physiology*
D011963:Receptors, GABA-A; D009118:Muscimol