title: Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus.
abstract: Cytopathic effects were produced in fetal rhesus monkey kidney (FRhK-4) cells 7 days postinfection by a serially BS-C-1-passaged strain of hepatitis A virus. Typical enterovirus cytopathology was produced by the HM-175 strain after 15 passages at 7-day intervals in BS-C-1 cells. No cytopathic effects were obtained after neutralization of virus with human anti-hepatitis A virus immunoglobulin G. Normal human serum had no effect on development of cytopathology. Maximum antigen and cDNA probe-based hybridization activity were associated with a CsCl gradient fraction having a density of 1.34 g/cm3. Large quantities of 27- to 30-nm virions typical of hepatitis A virus were associated with the same fraction. These data led to the conclusion that the observed cytopathology was caused by hepatitis A virus.
authors.affiliation: Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: A M
authors.initials: AM
authors.lastname: Nasser
authors.forename: T G
authors.initials: TG
authors.lastname: Metcalf
mesh_terms: D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003588:Cytopathogenic Effect, Viral; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000276:immunology; D006507:Hepatovirus / Q000235:genetics / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008854:Microscopy, Electron; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis; D011863:Radioimmunoassay; D012692:Serial Passage
chemical_list: D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; D012367:RNA, Viral