Isolation and characterization of the modification methylase M . SinI.
A sequence-specific modification methylase (M . SinI) was isolated and purified from Escherichia coli harboring a derivative of recombinant plasmid pSI4 (see accompanying manuscript: C. Karreman and A. de Waard, J. Bacteriol. 170:2527-2532, 1988), which contains a Salmonella infantis DNA insert. The enzyme uniquely methylates the internal deoxycytidylate residue in the nucleotide sequence GG(A/T)MeCC, thereby protecting DNA completely against cleavage by restriction endonuclease R . SinI or R . AvaII [GG(A/T)CC], and in part against cleavage by R . Sau96I (GGNCC).
Karreman
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C
Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, The Netherlands.
de Waard
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A
D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015257:DNA-Cytosine Methylases; D003841:Deoxycytidine / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008780:Methyltransferases / Q000302:isolation & purification*; D010957:Plasmids; D012475:Salmonella / Q000201:enzymology* / Q000235:genetics
D003841:Deoxycytidine; D004247:DNA; C056154:DNA modification methylase M.SinI; D015257:DNA-Cytosine Methylases; D008780:Methyltransferases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C038308:GGWCC-specific type II deoxyribonucleases