Detection of Chlamydia trachomatis by in situ hybridization with sulphonated total DNA.
In situ nucleic acid hybridization was applied to the detection of Chlamydia trachomatis on microscope slides by use of sulphonated total DNA as a probe. Visualization of labelled DNA was obtained using a commercial enzyme-linked monoclonal antibody. A mixture of paraformaldehyde and glutaraldehyde was found to be the best fixative. With high probe concentration (10 micrograms/ml), intracellular inclusions were detected as early as 8 h after inoculating the cell culture. Extracellular elementary bodies could also be detected. Five genital specimens were tested by in situ hybridization; the results were in agreement with those observed by culture.
Dutilh
B
B
Laboratoire de Bactériologie, Hôpital Pellegrin, Bordeaux, France.
Bébéar
C
C
Taylor-Robinson
D
D
Grimont
P A
PA
D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D003596:Cytosine; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005260:Female; D005404:Fixatives; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure; D008297:Male; D009693:Nucleic Acid Hybridization; D013447:Sulfites
D004269:DNA, Bacterial; D005404:Fixatives; D013447:Sulfites; D003596:Cytosine; C009279:sodium bisulfite