title: Molecular cloning and expression in E. coli of a Salmonella typhi porin gene.
abstract: Immunoscreening of a Salmonella typhi cosmid library in E. coli allowed the detection of clones producing a 36 kDa porin from S. typhi. The gene is efficiently expressed in an E. coli porin-less mutant and the protein is exported to the outer membrane envelope. Two clones which markedly differ in their level of expression have been isolated.
authors.affiliation: Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Santiago.
authors.forename: I
authors.initials: I
authors.lastname: Zaror
authors.forename: I
authors.initials: I
authors.lastname: Gómez
authors.forename: G
authors.initials: G
authors.lastname: Castillo
authors.forename: A
authors.initials: A
authors.lastname: Yudelevich
authors.forename: A
authors.initials: A
authors.lastname: Venegas
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D008970:Molecular Weight; D018272:Porins; D012485:Salmonella typhi / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018272:Porins; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases