title: Purification and characterization of human urine-derived digitalis-like factor.
abstract: We were able to purify a digitalis-like factor to apparent homogeneity from human urine based on the inhibitory effect on [3H]-ouabain binding to intact human erythrocytes. This ouabain displacing compound closely resembles ouabain in its polarity, molecular weight, non-peptidic nature and mode of action except for its UV absorbance spectrum. This compound sharing many biological activities of ouabain may be the endogenous ligand for the Na+, K+-ATPase and serve as a specific regulator of the sodium pump.
authors.affiliation: Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Goto
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Ishii
authors.forename: M
authors.initials: M
authors.lastname: Yoshioka
authors.forename: T
authors.initials: T
authors.lastname: Ishiguro
authors.forename: C
authors.initials: C
authors.lastname: Eguchi
authors.forename: T
authors.initials: T
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000494:pharmacology / Q000652:urine*; D001921:Brain / Q000378:metabolism; D002298:Cardenolides; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004077:Digoxin; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D010042:Ouabain / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012413:Rubidium / Q000097:blood; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium; D011188:Potassium