Efficient electroblotting of human very-low-density lipoproteins and low-density lipoproteins from composite gradient gels to nylon membranes.
Rinsing of gradient composite acrylamide/agarose gels with 1% Triton X-100 permits the efficient electrotransfer of very-low-density and low-density lipoproteins from the gels and does not appear to interfere with subsequent capture of the lipoproteins by charged nylon membranes. Overall efficiency of the transfer/capture process can approach 95% and does not appear to be significantly affected by total lipoprotein concentrations up to 5000 mg/dl. Direct immunoquantification of transferred apolipoproteins on the membrane is feasible as well. The nylon membranes used, however, must be pretested to ensure capture efficiency.
Lee
L T
LT
Department of Physiology, Louisiana State University Medical Center, New Orleans 70112.
Wong
L
L
Roheim
P S
PS
Thompson
J J
JJ
D003902:Detergents; D004563:Electrochemistry; D004586:Electrophoresis / Q000379:methods; D005782:Gels; D006801:Humans; D008077:Lipoproteins, LDL / Q000032:analysis*; D008079:Lipoproteins, VLDL / Q000032:analysis*; D008567:Membranes, Artificial; D009757:Nylons; D012015:Reference Standards; D015203:Reproducibility of Results
D003902:Detergents; D005782:Gels; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008567:Membranes, Artificial; D009757:Nylons