title: Human satellite-III DNA: an example of a "macrosatellite" polymorphism.
abstract: Human satellite III DNA contains a complex polymorphism, which appears to be TaqI-specific. Its likely cause is a two-step point mutation in the pentameric repeat TTCCA, typical of satellite III. Hybridization of the satellite-III sequence-related probe that demonstrates this polymorphism is directly attributable to clusters of "pure" pentameric TTCCA repeats in the genome. The sites of such repeats include the 3.4-kb fragment specific to the Y chromosome and a limited number of autosomes. The polymorphism arises from the latter and is likely to include chromosomes containing so-called K domain satellite III sequences found, for example, in chromosomes 9 and 15. Segregation of the polymorphic fragments appears to follow orthodox genetics.
authors.affiliation: School of Biological Sciences, Flinders University of South Australia.
authors.forename: C
authors.initials: C
authors.lastname: Fowler
authors.forename: R
authors.initials: R
authors.lastname: Drinkwater
authors.forename: J
authors.initials: J
authors.lastname: Skinner
authors.forename: L
authors.initials: L
authors.lastname: Burgoyne
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D010920:Placenta / Q000032:analysis; D011110:Polymorphism, Genetic; D011247:Pregnancy
chemical_list: D004276:DNA, Satellite; D004247:DNA; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases