Involvement of nucleoli and dense bodies in the intranuclear distribution of some capsid polypeptides in cells infected with herpes simplex virus type 1.
The distribution of capsid proteins induced by herpes simplex virus type 1 infection was determined at the ultrastructural level. The antiserum A to total capsid proteins and the anti-NC1 and NC2 sera, all labeled with gold particles, decorated the entire thickness of both empty capsids and nucleocapsids filled with viral DNA. On the other hand, an antibody to NC3,4 protein produced a heavy labeling concentrated almost entirely along the internal surface of empty capsids, whereas full capsids were not labeled. DNase digestion of "full" capsids did not restore anti-NC3,4 protein binding at this site. Published biochemical data concerning viral protein distribution in capsids are conflicting, but if NC3,4 protein is present in full capsids, we suggest that new binding forces between capsid proteins occurred at the time of insertion of viral DNA which might conceal the relevant antigenic sites of NC3,4 proteins. Capsid proteins were abundantly present in the viral nucleoplasm and in most constituents of the infected cells particularly some nucleoli and some but not all dense bodies. However, whereas anti-NC1 serum labeled nucleoli but not dense bodies, both anti-NC2 and anti-NC3,4 sera stained only dense bodies but not nucleoli. Inhibition of replication of viral DNA which entered the cell during the infective period did not inhibit the production of capsid proteins. Inhibition of protein synthesis in late infected cells did not alter the distribution of capsid proteins.
Puvion-Dutilleul
F
F
Groupe de Laboratoires, l'Institut de Recherches Scientifiques sur le Cancer, CNRS, Villejuif, France.
Cebrian
J
J
D000818:Animals; D002213:Capsid / Q000032:analysis* / Q000276:immunology; D002466:Cell Nucleolus / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis*; D002478:Cells, Cultured; D005347:Fibroblasts; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D011817:Rabbits; D018139:Simplexvirus / Q000502:physiology*; D014758:Viral Core Proteins
D014758:Viral Core Proteins