title: Expression of Theiler's murine encephalomyelitis virus protease 3C and polymerase 3D in Escherichia coli and characterization of monospecific sera.
abstract: Defined DNA fragments of cloned Theiler's murine encephalomyelitis virus genome were used to construct procaryotic recombinant plasmids expressing viral genes 3C and 3D. In these plasmids (pEX-EMBL vectors), viral sequences are fused in-phase behind the Escherichia coli lac Z' gene which is under the control of the inducible lambda Pr promoter. Partially purified fusion proteins were used to immunize Balb/c mice. Sera monospecific for the viral protease 3C and polymerase 3D were obtained. These sera detected their corresponding antigens in situ in infected BHK cells using immunocytochemical reactions.
authors.affiliation: Unité Virus Lents, UA 1157 CNRS, Institut Pasteur, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Ozden
authors.forename: C
authors.initials: C
authors.lastname: Aubert
authors.forename: M
authors.initials: M
authors.lastname: Brahic
mesh_terms: D000086042:3C Viral Proteases; D000818:Animals; D002460:Cell Line; D011232:Chemical Precipitation; D006224:Cricetinae; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000235:genetics* / Q000276:immunology; D004770:Enterovirus / Q000235:genetics*; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D004680:Maus Elberfeld virus / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D010447:Peptide Hydrolases / Q000235:genetics* / Q000276:immunology; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014764:Viral Proteins
chemical_list: D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase; D010450:Endopeptidases; D010447:Peptide Hydrolases; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases