Effect of D-penicillamine on the expression and propagation of the human immunodeficiency virus by H9 T-lymphoblastoid cells.
The effect of D-penicillamine on the replication of the Human Immunodeficiency Virus (HIV) in H9 T-lymphoblastoid cells was evaluated. HIV-infected and uninfected H9 cells were incubated for a period of 6 weeks with various concentrations of D-penicillamine. During the first 8 days, D-penicillamine (at concentrations of 0-150 micrograms/ml) did not influence growth and viability of H9/H9-IIIB cells. However, long term cultures and higher doses of the drug caused a decay in cell number and viability of the cultured cells. Up to the 8th day of culture, D-penicillamine did not exert an inhibiting effect on the number of HIV-infected cells but did suppress HIV-antigen production by up to 29%. After 6 weeks, in addition to a decrease of the relative viral antigen concentration from 390-500 ng/ml in the medium control to 210-320 ng/ml in cultures containing 150 micrograms D-penicillamine/ml, D-penicillamine also reduced the percentage of HIV-positive cells. Thus our results demonstrate an antiviral effect of D-penicillamine in H9 cultures.
Bitterlich
G
G
Institute for Hygiene, University of Innsbruck, Austria.
Larcher
C
C
Sölder
B
B
Hengster
P
P
Myones
B L
BL
Hofbauer
J
J
Schulz
T F
TF
Wachter
H
H
Dierich
M P
MP
D002478:Cells, Cultured; D006678:HIV / Q000187:drug effects* / Q000502:physiology; D015488:HIV Antigens / Q000032:analysis; D006801:Humans; D010396:Penicillamine / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013601:T-Lymphocytes / Q000166:cytology / Q000382:microbiology*; D013936:Thymidine / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D015488:HIV Antigens; D010396:Penicillamine; D013936:Thymidine