Comparison of the production of eicosanoids by human and rat peritoneal macrophages and rat Kupffer cells.
Human and rat peritoneal macrophages and rat Kupffer cells were labelled with [1-14C] arachidonic acid and stimulated with the calcium ionophore A23187. The metabolites formed were separated by high pressure liquid chromatography (HPLC). Human peritoneal macrophages formed especially leukotriene B4, 5-hydroxy-6,8,11,14 eicosatetraenoic acid and small amounts of leukotriene C4 and thromboxane B2, 12-hydroxy-5,8,10 heptadecatrienoic acid and 6-keto-prostaglandin F1 alpha, whereas rat peritoneal macrophages mainly produced cyclooxygenase products and in particular thromboxane B2 and 12-hydroxy-5,8,10 heptadecatrienoic acid. Rat Kupffer cells synthesized mainly cyclooxygenase products such as prostaglandin F2 alpha, prostaglandin D2 and prostaglandin E2. These results indicate that the profile of eicosanoids production by macrophages is dependent both on the species and on the tissue from which the macrophage is derived.
Ouwendijk
R J
RJ
Department of Internal Medicine, Erasmus University Rotterdam, The Netherlands.
Zijlstra
F J
FJ
van den Broek
A M
AM
Brouwer
A
A
Wilson
J H
JH
Vincent
J E
JE
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001202:Ascitic Fluid / Q000166:cytology*; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007728:Kupffer Cells / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis; D008084:Lipoxygenase / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology*; D010533:Peritoneal Lavage; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011453:Prostaglandins / Q000096:biosynthesis; D051381:Rats; D013189:SRS-A / Q000096:biosynthesis; D013929:Thromboxane B2 / Q000096:biosynthesis
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D011453:Prostaglandins; D013189:SRS-A; D007975:Leukotriene B4; D016718:Arachidonic Acid; D013929:Thromboxane B2; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases