Interaction of alpha-acetoxy-N-nitrosopyrrolidine with DNA assessed by loss of restriction endonuclease recognition sites and formation of apurinic/apyrimidinic sites.
alpha-Acetoxy-N-nitrosopyrrolidine (AcO-NPYR), in the presence of esterase, was reacted with the plasmid pBR322. The AcO-NPYR-treated plasmid was nicked by reaction with spermidine, suggesting the presence of apurinic sites. The treated plasmid also blocked the action of the restriction endonuclease BglI and, to a lesser extent, DraI. The recognition sequences of these restriction enzymes suggest that AcO-NPYR interacts preferentially with guanine and/or cytosine bases in DNA.
Gunhouse
B W
BW
Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute, Canada.
Milligan
J R
JR
Archer
M C
MC
D001080:Apurinic Acid / Q000378:metabolism*; D003596:Cytosine; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004128:Dimethylnitrosamine; D004950:Esterases / Q000378:metabolism; D006147:Guanine; D008770:Methylnitrosourea; D009242:N-Nitrosopyrrolidine / Q000031:analogs & derivatives; D009602:Nitrosamines; D010957:Plasmids; D011119:Polynucleotides / Q000378:metabolism*; D013095:Spermidine
D009602:Nitrosamines; D011119:Polynucleotides; D001080:Apurinic Acid; C036669:alpha-acetoxy-N-nitrosopyrrolidine; D006147:Guanine; D008770:Methylnitrosourea; D003596:Cytosine; D004247:DNA; D004950:Esterases; D004262:DNA Restriction Enzymes; D004128:Dimethylnitrosamine; D009242:N-Nitrosopyrrolidine; D013095:Spermidine