Separation of 5'-nucleotide phosphodiesterase isoenzymes of human serum by anion exchange high pressure liquid chromatography.
5'-Nucleotide phosphodiesterase isoenzymes (5'-NPD; E.C. 3.1.4.1) of human serum were separated by anion exchange high pressure liquid chromatography. In sera of healthy donors five activities could be separated, designated as Z, A, B, C and D. Under certain pathological conditions a further isozyme E occurred. With the exception of Z all isoenzymes could be related to the activities first described by Tsou and coworkers in normal sera (Tsou et al. J Histochem Cytochem 1973; 21: 402). These authors identified by means of native gel electrophoresis the isoenzymes 5'-NPD-I, 5'-NPD-II, 5'-NPD-III and 5'-NPD-IV, designated in the reverse order of their electrophoretic mobility. By using this gel technique we characterized the activities A, B, C and D as 5'-NPD-II, -III, -IV, and -I, respectively. Activity E corresponded to an isozyme which did not migrate into the gel (designated as 5'-NPD-O).
Lüthje
J
J
Institut für Physiologische Chemie, Universität Erlangen-Nürnberg, (FRG).
Ogilvie
A
A
D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000097:blood*
D007527:Isoenzymes; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I