The yeast UASG is a transcriptional enhancer in human HeLa cells in the presence of the GAL4 trans-activator.
The yeast trans-activator protein GAL4, when expressed in HeLa cells, stimulates transcription from several class B (II) eukaryotic promoters containing GAL4 binding sites either as the full UASG or as synthetic 17-mers. The characteristics of this activation are indistinguishable from those of the SV40 enhancer. Transcription was similarly stimulated from either complex promoter regions containing multiple upstream elements or from a simple promoter region composed of only a TATA box. Addition of a 17-mer GAL4 binding site to the SV40 enhancer resulted in a synergistic enhancement of transcription in the presence of GAL4. Furthermore, chimeras of the human estrogen receptor DNA binding domain and either GAL4 or GCN4 activating "acidic" regions can activate a promoter region controlled by an estrogen-responsive enhancer. Together, these data indicate that the molecular mechanisms responsible for transcriptional enhancement have been conserved from yeast to man.
Webster
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Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médecine, Strasbourg, France.
Jin
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JR
Green
S
S
Hollis
M
M
Chambon
P
P
D000818:Animals; D004271:DNA, Fungal / Q000235:genetics*; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D004742:Enhancer Elements, Genetic; D004967:Estrogens / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics*; D005914:Globins / Q000235:genetics; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011817:Rabbits; D011960:Receptors, Estrogen / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D029701:Saccharomyces cerevisiae Proteins; D013539:Simian virus 40 / Q000235:genetics; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013936:Thymidine / Q000235:genetics; D014157:Transcription Factors; D014158:Transcription, Genetic; D014162:Transfection
D004271:DNA, Fungal; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D004967:Estrogens; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D011960:Receptors, Estrogen; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005914:Globins; D013936:Thymidine