A rapid method for the detection of papillomavirus in warts: the frequency of virus detection in various types of warts.
A rapid method was devised for the detection of virus particles in wart specimens. The upper layer of warts was cut perpendicularly to the surface, and the freshly cut surface was lightly touched to an electron microscope grid. The grid was then stained with a small drop of phosphotungstate and observed electron microscopically. On the specimen grid thus prepared, papillomavirus particles were easily discriminated from tissue debris. Papillomavirus particles were detected in 71% of verrucae plantares, 78% of verrucae palmares, 50% of verrucae vulgares and 75% of condylomata acuminata by the present method.
Yabe
Y
Y
Department of Virology, Okayama University Medical School, Japan.
Kuramitsu
M
M
D003218:Condylomata Acuminata / Q000382:microbiology / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D027383:Papillomaviridae / Q000648:ultrastructure*; D014773:Virology / Q000379:methods; D014860:Warts / Q000382:microbiology* / Q000648:ultrastructure