Pressure induced structural fluctuations in hemoglobin, studied by EPR-spectroscopy.
A quartz based cavity for pressure dependent EPR measurements on liquid samples allowing pressures up to 0.6 GPa was constructed. First investigations with this setup were done on spin labeled horse hemoglobin derivatives both in ferric and ferrous state of oxidation. The second derivative EPR spectra show changes of the label's mobility, which are not correlated with spin state changes of the Fe-porphyrin complex, but which point out structural fluctuations inside the globin protein matrices.
Paul
J
J
Institut für Biophysik, Universität Witten/Herdecke, Bundesrepublik Deutschland.
von Goldammer
E
E
Wenzel
H R
HR
D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D006454:Hemoglobins; D008706:Methemoglobin; D011312:Pressure; D013113:Spin Labels
D003497:Cyclic N-Oxides; D006454:Hemoglobins; D013113:Spin Labels; C003028:N-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)iodoacetamide; D008706:Methemoglobin