The relationship between superoxide generation, cytochrome b and oxygen in activated neutrophils.
The O2 affinity for O2.- generation (i.e. O2 tension at which 50% of the O2-saturated activity was observed) by rat neutrophils activated by the chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine) plus cytochalasin B was measured as 5.4 microM (+/- 1.4, n = 5). Similarly, the O2 tension at which cytochrome b was oxidized to 50% of its aerobic steady-state level in activated cells was 4.7 microM (+/- 1.0, n = 3): in non-activated cells the corresponding value for cytochrome b oxidation was 11.4 microM (+/- 2.8, n = 3). It is proposed that O2 depletion at inflammatory sites may limit oxidant generation by activated neutrophils and thus reduce oxidant damage to surrounding tissues.
Edwards
S W
SW
Department of Biochemistry, University of Liverpool, England.
Lloyd
D
D
D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology; D003573:Cytochrome b Group / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D013053:Spectrophotometry; D013481:Superoxides / Q000378:metabolism*
D003573:Cytochrome b Group; D013481:Superoxides; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D010100:Oxygen