Separation of human plasma factor IX from HTLV-I or HIV by immunoaffinity chromatography using conformation-specific antibodies.
Immunoaffinity chromatography using conformation-specific antibodies yields pure factor IX from human plasma in a single rapid, facile purification step. We evaluated this technique to determine whether factor IX can be separated from human T cell leukemia virus-I (HTLV-I) and human immunodeficiency virus (HIV) in plasma supplemented with these viruses. Viral content was determined with an enzyme-linked immunosorbent (ELISA) assay sensitive to 50 ng viral protein. Both HTLV-I and HIV coeluted with unbound protein. Neither HTLV-I nor HIV was detected in purified factor IX. We conclude that, to the limits of detection, factor IX purified by this method is free of viral contamination.
Limentani
S A
SA
Department of Medicine, New England Medical Center, Boston, MA.
Furie
B C
BC
Poiesz
B J
BJ
Montagna
R
R
Wells
K
K
Furie
B
B
D000906:Antibodies; D002846:Chromatography, Affinity / Q000379:methods; D017977:Deltaretrovirus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005164:Factor IX / Q000276:immunology / Q000302:isolation & purification*; D006678:HIV / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D011487:Protein Conformation
D000906:Antibodies; D005164:Factor IX