title: Pulmonary edema after pulmonary artery occlusion and reperfusion.
abstract: We examined the basis of reperfusion-induced pulmonary edema produced by pulmonary artery occlusion and subsequent reperfusion. After a 24-h period of occlusion of a rabbit pulmonary artery followed by a 2-h period of reperfusion, the lungs were removed from the animal and perfused with a 0.5 g% Ringer's-albumin solution. An increase in lung weight was observed within 60 min compared with control lungs (i.e., lungs subjected to pulmonary arterial occlusion but not reperfusion) (p less than 0.05). Shorter periods of occlusion (6 or 12 h) did not result in edema, which suggests that a period of ischemia was required for the reperfusion-induced pulmonary edema. The extravascular lung water content also increased in the contralateral lung (i.e., the lung not subjected to pulmonary arterial occlusion and reperfusion). The capillary filtration coefficient increased in reperfused lungs compared with controls (p less than 0.05), indicating an increase in lung vascular permeability following reperfusion. Infusion of allopurinol (a xanthine oxidase inhibitor) and superoxide dismutase during the reperfusion period prevented the increases in lung weight and vascular permeability; infusion of catalase was ineffective. We conclude that pulmonary reperfusion following pulmonary artery occlusion increases pulmonary vascular permeability, which is mediated by the generation of oxidants.
authors.affiliation: Department of Physiology, Albany Medical College of Union University, New York.
authors.forename: M J
authors.initials: MJ
authors.lastname: Horgan
authors.forename: H
authors.initials: H
authors.lastname: Lum
authors.forename: A B
authors.initials: AB
authors.lastname: Malik
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002404:Catheterization; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D008168:Lung / Q000201:enzymology / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011651:Pulmonary Artery / Q000473:pathology; D011654:Pulmonary Edema / Q000209:etiology*; D011669:Pulmonary Wedge Pressure; D011817:Rabbits; D015427:Reperfusion Injury / Q000150:complications* / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000494:pharmacology; D014968:Xanthine Dehydrogenase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D013482:Superoxide Dismutase; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase