Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells.
The v-myc oncogene can induce tumours in haematopoietic, mesenchymal and epithelial tissues. The corresponding c-myc proto-oncogene can contribute to the genesis and/or the progression of an equally wide variety of tumours when activated by retroviral insertions, chromosomal translocations or gene amplification. The c-myc gene product is a DNA-binding, nuclear phosphoprotein that is involved in the control of cell proliferation and possibly in DNA synthesis. The replication of Simian virus 40 (SV40) is a useful model system to study eukaryotic DNA replication as the virus relies almost entirely on cellular DNA replication apparatus. The SV40-based vector, pSVEpR4, replicates poorly in the human BJAB lymphoma line and in most human cells, but replicates well in Burkitt lymphoma lines, which have fused immunoglobulin and c-myc genes, resulting in high c-myc expression. Cotransfection of the BJAB cells with a c-myc-expressing construct (pI4-P6) increased the replication of pSVEpR4 tenfold. Our findings indicate that overexpression of the c-myc gene product allows the replication of SV40 in human lymphoma cells, suggesting that c-myc is involved in the control of replication.
Classon
M
M
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Henriksson
M
M
Sümegi
J
J
Klein
G
G
Hammarskjöld
M L
ML
Hammaskjöld
M L
ML
D000957:Antigens, Viral, Tumor / Q000235:genetics; D002051:Burkitt Lymphoma; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D008223:Lymphoma; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas