Human B and T lymphocytes convert leukotriene A4 into leukotriene B4.
Incubation of human tonsillar B lymphocytes and peripheral blood T lymphocytes with leukotriene A4 led to the formation of leukotriene B4. The purity of these cell suspensions was more than 99%, containing less than 0.5% monocytes. Incubation of purified B or T lymphocytes with the calcium ionophore A23187 did not lead to the formation of any detectable amounts of leukotrienes. Several established cell lines of B and T lymphocytic origin were also found to convert leukotriene A4 into leukotriene B4, showing that monoclonal lymphocytic cells possess leukotriene A4 hydrolase activity.
Odlander
B
B
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
Jakobsson
P J
PJ
Rosén
A
A
Claesson
H E
HE
D001095:Arachidonic Acids / Q000097:blood*; D001402:B-Lymphocytes / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D017572:Leukotriene A4; D007975:Leukotriene B4 / Q000097:blood*; D013601:T-Lymphocytes / Q000378:metabolism*
D001095:Arachidonic Acids; D017572:Leukotriene A4; D007975:Leukotriene B4; D000001:Calcimycin