Cyclosporin A and prednisolone do not inhibit the expression of high-affinity receptors for interleukin 2.
To elucidate the mechanisms of action of cyclosporin A (CyA) and prednisolone (pred), we investigated the effects of these immunosuppressive drugs on the expression of receptors for interleukin 2 (IL-2) as well as the effect of exogenous IL-2 on CyA-inhibited and pred-inhibited responses. Pred-induced inhibition of T cell proliferative responses could be reversed by addition of exogenous IL-2. In contrast, exogenous IL-2 could not overcome CyA-mediated inhibition of T cell proliferation. Receptors for IL-2, as measured by the expression of Tac antigen, were slightly decreased by CyA and pred. However, expression of the biologically active, high-affinity IL-2 receptors was not diminished. These data suggest that pred-mediated inhibition occurs at the level of the IL-2 system, whereas CyA affects the proliferative mechanism of the T cell itself.
Bloemena
E
E
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Van Oers
M H
MH
Weinreich
S
S
Schellekens
P T
PT
D000328:Adult; D000954:Antigens, Surface / Q000276:immunology; D002478:Cells, Cultured; D003524:Cyclosporins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008875:Middle Aged; D008938:Mitosis / Q000187:drug effects; D011239:Prednisolone / Q000494:pharmacology*; D011948:Receptors, Antigen, T-Cell / Q000187:drug effects*; D011971:Receptors, Immunologic / Q000187:drug effects*; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000276:immunology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000954:Antigens, Surface; D003524:Cyclosporins; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D011239:Prednisolone