Organization of the human pro-alpha 2(I) collagen gene.
Sixty kilobases of cloned DNA containing the entire human pro-alpha 2(I) collagen gene and 22 kilobases of flanking sequences have been isolated. Like the homologous avian gene, the 1366 amino acid residues of the human pre-pro-alpha 2(I) chain are encoded by 52 exons, whose relative locations and sizes have been determined. Analysis of the 5'- and 3'-untranslated regions have confirmed their exact lengths, as well as conclusively established the nature of five polymorphic mRNA transcripts.
de Wet
W
W
Department of Biochemistry, Potchefstroom University, South Africa.
Bernard
M
M
Benson-Chanda
V
V
Chu
M L
ML
Dickson
L
L
Weil
D
D
Ramirez
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D011347:Procollagen / Q000235:genetics*
D011347:Procollagen; D004247:DNA; D004262:DNA Restriction Enzymes