title: Time sequence of osteoinduction and osteostimulation elicited by biologic bone replacement materials.
abstract: The combinations of a biologic bone replacement material (BRM), bone gelatine (BG), a matrix extract, with one of the synthetic BRMs, either powderised beta-tricalcium-phosphate (TCP) or particulate hydroxyapatite (HA), as well as BG alone were implanted in muscle pouches and diaphyseal femoral drill-holes in rats. Implantation between one and 21 days. Evaluation by histomorphometry of PMMA embedded undecalcified specimen. In heterotopic implantation bone formation occurred earliest in the BG/TCP combination with highest values for volume density bone and osteoid after 21 days. In orthotopic implantation starting between days 9 and 13 the BG/HA combination stimulated intense woven bone formation with massive ingrowth of vital bone into the HA pores. The biodegradable TCP, by activating the monocyte-/macrophage-system for its degradation, obviously enhanced the already ongoing immunologic reaction to allogeneic BG, thus impeding bone formation and repair.
authors.affiliation: Zentrum der Chirurgie, Universitaetsklinik Frankfurt, West-Germany.
authors.forename: J M
authors.initials: JM
authors.lastname: Rueger
authors.forename: H R
authors.initials: HR
authors.lastname: Siebert
authors.forename: M
authors.initials: M
authors.lastname: Dohr-Fritz
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
authors.forename: A
authors.initials: A
authors.lastname: Pannike
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones; D002130:Calcium Phosphates; D017886:Durapatite; D005780:Gelatin; D006882:Hydroxyapatites; D010012:Osteogenesis; D019736:Prostheses and Implants; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006882:Hydroxyapatites; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D005780:Gelatin; D017886:Durapatite; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous