Processing and presentation of cell-associated varicella-zoster virus antigens by human monocytes.
To determine whether viral antigens associated with infected cells were processed for presentation to T cells, we cultured human blood mononuclear cells (MNC) from varicella-zoster virus (VZV) immune donors with VZV-infected fibroblasts of known HLA type which had been fixed in 0.05% glutaraldehyde. After 7-8 days of culture thymidine uptake by T4+ cells exceeded that of T8+ cells. Stimulated cells were depleted of adherent cells and restimulated with VZV-infected fibroblasts from donors matched or unmatched with the responder for HLA type in the presence or absence of fresh adherent cells. Proliferation of the VZV-specific blasts required the presence of adherent cells matched with the responder lymphocytes for HLA-DR; conversely, the VZV specific response was not restricted by the MHC of the fibroblasts used in the restimulation assay. Preincubation of the adherent cells with chloroquine inhibited the proliferative response in a dose-dependent manner. These results suggest that VZV antigens on infected cells may be processed by monocytes for presentation to T cells.
Pontesilli
O
O
Barbara Davis Center for Childhood Diabetes, (University of Colorado School of Medicine), Denver 80262.
Carotenuto
P
P
Levin
M J
MJ
Suez
D
D
Hayward
A R
AR
D000328:Adult; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology*; D002455:Cell Division; D002738:Chloroquine / Q000494:pharmacology; D005347:Fibroblasts / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D009000:Monocytes / Q000276:immunology*
D000954:Antigens, Surface; D000956:Antigens, Viral; D006680:HLA Antigens; D002738:Chloroquine