Identification of a UV-induced trans-acting protein that stimulates polyomavirus DNA replication.
Previous studies provided indirect evidence that the ability of a variety of DNA-damaging agents to induce asynchronous polyomavirus DNA replication in the H3 rat fibroblast cell line is mediated by a trans-acting factor. Using an erythrocyte insertion technique to introduce protein fractions from UV-irradiated cells into unirradiated H3 cells, we have now obtained evidence that this factor is a 60-kilodalton protein. These findings provide evidence that DNA damage in mammalian cells induces a factor that can alter the replication of a viral DNA.
Ronai
Z A
ZA
Comprehensive Cancer Center, Columbia University, New York, New York 10032.
Weinstein
I B
IB
D000818:Animals; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004261:DNA Replication; D005347:Fibroblasts / Q000032:analysis* / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects; D015696:Gene Products, tat; D008970:Molecular Weight; D011120:Polyomavirus / Q000502:physiology*; D051381:Rats; D014157:Transcription Factors / Q000302:isolation & purification* / Q000502:physiology; D014466:Ultraviolet Rays; D014779:Virus Replication
D015696:Gene Products, tat; D014157:Transcription Factors; D004247:DNA