title: Expression of an early Epstein-Barr virus antigen (EA-D) in E. coli. Brief report.
abstract: The 1.34 kb BcII-BgIII-fragment of the BamHI-M region of Epstein-Barr virus genome, comprising the complete BMRF1 open reading frame, was cloned into the tryptophan regulated E. coli expression vector pATH1. The resulting fusion protein, having a molecular weight of 80 kd, is recognized not only by anti-early antigen (EA)-positive human sera but also by the monoclonal antibody R3 directed against the diffuse component of EA (EA-D). A possible use for this fusion protein as an indicator protein in diagnosis of IgA antibodies against EA-D is presented.
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Federal Republic of Germany.
authors.forename: D
authors.initials: D
authors.lastname: Roeckel
authors.forename: H
authors.initials: H
authors.lastname: Boos
authors.forename: N
authors.initials: N
authors.lastname: Mueller-Lantzsch
mesh_terms: D000956:Antigens, Viral / Q000235:genetics*; D002051:Burkitt Lymphoma / Q000382:microbiology; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D014158:Transcription, Genetic
chemical_list: D000956:Antigens, Viral; C033305:Epstein-Barr virus early antigen; D004262:DNA Restriction Enzymes