title: [Sex determination at the DNA level. Are inter-individual differences in male sex specific quotients to be expected?].
abstract: A determination of sex was performed for 23 male individuals using sex-specific DNA-probe (Y) (pJA 1143) and total human DNA as DNA-probes (A). The DNA-probes were P32 marked. Applying dot-blot-hybridisation black spots are obtained, the intensity of which may be quantified by a densimeter. The ratio (A/Y) of the intensity of the spots related to total human DNA (A) and to the sex-specific DNA-probe (Y) was calculated. DNA-dilutions of one male individual were prepared in order to determine the ratio (A/Y) for different DNA-concentrations. It was evident that the deviations for the sex-specific quotients of the DNA-dilutions amounts so large-scaled as compared to the quotients of the 23 male individuals, that the method of dot-blot-hybridisation appears not to be applicable to detect an individual component of a particular spot.
authors.forename: E
authors.initials: E
authors.lastname: Josephi
authors.forename: J
authors.initials: J
authors.lastname: Teifel-Greding
authors.forename: E
authors.initials: E
authors.lastname: Tutsch-Bauer
authors.forename: E
authors.initials: E
authors.lastname: Schuller
authors.forename: E
authors.initials: E
authors.lastname: Liebhardt
mesh_terms: D001483:Base Sequence; D015342:DNA Probes; D005260:Female; D006801:Humans; D008297:Male; D012732:Sex Determination Analysis; D014998:Y Chromosome
chemical_list: D015342:DNA Probes
vernacular_title: Geschlectsbestimmung auf DNA-Ebene. Ist mit inter-individuellen Abweichungen beim männlichen geschlechtsspezifischen Quotienten zu rechnen?