Contrasting effects of ACTH and cyanoketone on delta 5-3 beta-hydroxysteroid dehydrogenase activity in interrenal glands of tadpoles of Rana catesbeiana in vitro.
The present communication describes an investigation of stimulation and inhibition of delta 5-3 beta-hydroxysteroid dehydrogenase in interrenal glands of tadpoles of Rana catesbeiana. Frozen sections of interrenal glands, together with kidneys, were prepared histochemically for assay of delta 5-3 beta-HSD activity. Concentrations of 0.01, 0.1, 1, and 10 IU/ml of ACTH or of 0.01, 0.1, 1, and 10 micrograms/ml of cyanoketone were added to the incubation media. The reaction products of the histochemically prepared slides, in terms of absorbance, were scanned at a defined area with a computerized microscope spectrophotometer. The results indicate that ACTH causes a significant dose-response stimulation of delta 5-3 beta-HSD activity in tadpole interrenals; cyanoketone, on the other hand, causes significant dose-dependent inhibition.
Yu
N W
NW
Department of Biomorphics, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Ku
H H
HH
Chang
L T
LT
Hsu
C Y
CY
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000311:Adrenal Glands / Q000201:enzymology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000737:Androstenols / Q000494:pharmacology*; D000818:Animals; D003489:Cyanoketone / Q000494:pharmacology*; D006651:Histocytochemistry; D007401:Interrenal Gland / Q000166:cytology / Q000201:enzymology*; D007700:Kinetics; D011892:Rana catesbeiana
D000737:Androstenols; D003489:Cyanoketone; D000324:Adrenocorticotropic Hormone; D015096:3-Hydroxysteroid Dehydrogenases; C031954:delta(5)-3 beta-hydroxysteroid dehydrogenase