E1a inducibility of the adenoviral early E2a promoter is determined by specific combinations of sequence elements.
By transient expression analysis in HeLa cells of adenovirus-2 E2a early (E2aE) promoter mutants and hybrid E2aE-beta-globin gene constructs, we demonstrate the existence of three nucleotide (nt) sequence elements involved in the E1a-responsiveness of the E2aE transcription unit: element I, localized within a segment (nt -13 to +62) surrounding the major E2aE cap site (nt + 1); element II (between nt -71 and -29), and element III (between nt -146 and -86). Each element is unable by itself to confer E1a responsiveness. Only combinations of sequences including elements I and II (spanning nt -71 and +62) or II and III (spanning nt -146 and -29) ensure maximal inducibility, for which element II appears of central importance.
Zajchowski
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Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire, Strasbourg, France.
Boeuf
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Kédinger
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D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012334:RNA, Neoplasm / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D017456:Adenovirus Early Proteins; D009856:Oncogene Proteins, Viral; D012334:RNA, Neoplasm; D012367:RNA, Viral; D004262:DNA Restriction Enzymes