title: Changes in inositol polyphosphate-sensitive calcium exchange in aortic smooth muscle cells in vitro.
abstract: Cells that expressed the muscle-specific intermediate filament protein desmin were cultured from the aorta of Fischer 344 rats. When the cultured cells were extracted with digitonin, they accumulated 45Ca2+ from the incubation medium in a manner that was stimulated by ATP and released subsequently by exposure to the Ca2+ ionophore A23187. Ca2+ bound in the presence of ATP was also released by exposure to inositol 1,4,5-trisphosphate (IP3). Like contraction in some kinds of smooth muscle, IP3 released Ca2+ in either the absence or the presence of the ATPase-inhibitor ruthenium red. When the responsiveness of digitonin-extracted cells cultured from 3-, 12-, and 24-month-old rats was compared, cells from the youngest group released only about one-half as much Ca2+ as cells from the 12- or 24-month-old rats. The results suggest that in the rat there are changes during maturation in the responsiveness to inositol polyphosphates of intracellular compartments that sequester Ca2+ for stimulus-contraction coupling in the aortic smooth muscle cell. These changes, characterized in smooth muscle cells in vitro, might contribute to the way vascular responsiveness is regulated in vivo.
authors.affiliation: Biochemical Pharmacology Laboratory, VA Medical Center, Sepulveda, California 91343.
authors.forename: M
authors.initials: M
authors.lastname: Fittingoff
authors.forename: J F
authors.initials: JF
authors.lastname: Krall
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology; D007295:Inositol Phosphates / Q000494:pharmacology*; D007474:Ion Exchange / Q000187:drug effects; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D013403:Sugar Phosphates / Q000494:pharmacology*
chemical_list: D002712:Chlorides; D007295:Inositol Phosphates; D013403:Sugar Phosphates; D000001:Calcimycin; D000255:Adenosine Triphosphate; D008094:Lithium; D018021:Lithium Chloride; D002118:Calcium