A diacylglycerol kinase inhibitor, R59022, potentiates secretion by and aggregation of thrombin-stimulated human platelets.
The diacylglycerol kinase inhibitor R59022 (10 microM) potentiates secretion and aggregation responses in human platelets challenged with sub-maximal concentrations of thrombin. Potentiation correlates closely with increased formation of diacylglycerol, increased phosphorylation of a 40 kDa protein, a known substrate for protein kinase C, and with decreased formation of phosphatidic acid, the product of diacylglycerol kinase. Phosphorylation of myosin light chains, formation of inositol phosphates and the mobilization of Ca2+ by thrombin are not affected by R59022 (10 microM). These data support a role for protein kinase C in platelet aggregation and secretion, and provide further evidence that endogenous diacylglycerols bring about the activation of this enzyme. These data also add further argument against a role for phosphatidic acid in platelet activation.
Nunn
D L
DL
Department of Pharmacology, University of Oxford, U.K.
Watson
S P
SP
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000097:blood; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D010712:Phosphatidic Acids / Q000097:blood; D010766:Phosphorylation; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D010974:Platelet Aggregation / Q000187:drug effects*; D011744:Pyrimidinones / Q000494:pharmacology*; D012701:Serotonin / Q000097:blood; D013844:Thiazoles / Q000494:pharmacology*; D013917:Thrombin / Q000494:pharmacology*
C011439:1,2-diacylglycerol; D004075:Diglycerides; D010712:Phosphatidic Acids; D011744:Pyrimidinones; D013844:Thiazoles; D012701:Serotonin; D000001:Calcimycin; C047505:R 59022; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase; D013917:Thrombin; D002118:Calcium