Experimental analysis of concurrent viral and bacterial infection in the mouse.
The roles of both microorganisms in combined murine cytomegalovirus (MCMV) and Klebsiella pneumoniae (KP) infection in the mouse, and host response to the infection were investigated. Increased mortality in mixed MCMV and KP infected mice was observed, and this was due to uncontrolled growth of bacteria in the peritoneal cavity, and severe generalized infection. Neutrophil response to growth of KP was defective in MCMV infected mice. While the initial clearance of KP from the blood was more efficient in MCMV-infected mice, it did not protect the mice against KP infection. In the in vitro experiments, the neutrophil chemotaxis of MCMV-infected mice to KP was found to be lowered. Tissue extract and serum of MCMV-infected mice exhibited chemotactic activity for neutrophils, and, at the same time contained some factor(s) which reduced chemotaxis of normal neutrophils to KP.
Hashimoto
K
K
Department of Microbiology, School of Medicine, Tokai University, Kanagawa, Japan.
D000818:Animals; D002634:Chemotaxis, Leukocyte; D003586:Cytomegalovirus Infections / Q000150:complications*; D007710:Klebsiella Infections / Q000150:complications*; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009504:Neutrophils / Q000473:pathology; D010529:Peritoneal Cavity / Q000382:microbiology / Q000473:pathology; D011014:Pneumonia / Q000150:complications*; D013047:Specific Pathogen-Free Organisms