Phospholipase activation, free fatty acids and the proton permeability of a biological membrane.
The rate of collapse of a proton gradient across the apical membrane of rat kidney proximal tubule increases upon treatment with calcium, mercuric chloride and mellitin, substances which activate phospholipase A2. Treatment with phospholipase A2 or oleic acid also enhances the rate of proton gradient dissipation. Membrane water permeability is not affected. This phenomenon may have implications in pathological states arising from ischemia or toxic exposure.
Lachowiez
R M
RM
Department of Chemistry, State University of New York, Binghamton 13901.
Hammoud
N M
NM
Teibel
J L
JL
Dix
J A
JA
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism*; D008555:Melitten / Q000494:pharmacology; D008627:Mercuric Chloride / Q000494:pharmacology; D008871:Microvilli / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism* / Q000494:pharmacology; D054467:Phospholipases A2; D011522:Protons; D051381:Rats
D005230:Fatty Acids, Nonesterified; D009829:Oleic Acids; D011522:Protons; D008555:Melitten; D019301:Oleic Acid; D000001:Calcimycin; D008627:Mercuric Chloride; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium