title: Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli.
abstract: Nucleotide sequence analysis of the gene (spa) for staphylococcal protein A (SPA) from Staphylococcus aureus strain Cowan 1 (NCTC8530) shows that the sequence differs from previously reported SPA nucleotide sequences, especially in the number of repeat units in the cell-wall-binding region of the gene. Dot matrix comparison with streptococcal protein G and the macrophage receptor for the constant fragment (Fc) of immunoglobulins shows a limited but significant homology. The homology to the latter probably identifies the Fc-binding region in the immunoglobulin-binding domains of SPA. Enhanced production of SPA in Escherichia coli was achieved using the lac promoter immediately upstream from the spa gene.
authors.affiliation: Microbial Technology Laboratory, PHLS, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts, U.K.
authors.forename: H L
authors.initials: HL
authors.lastname: Shuttleworth
authors.forename: C J
authors.initials: CJ
authors.lastname: Duggleby
authors.forename: S A
authors.initials: SA
authors.lastname: Jones
authors.forename: T
authors.initials: T
authors.lastname: Atkinson
authors.forename: N P
authors.initials: NP
authors.lastname: Minton
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D013205:Staphylococcal Protein A; D004262:DNA Restriction Enzymes