title: Meiotic expression of human ornithine transcarbamylase in the testes of transgenic mice.
abstract: In an attempt to use mouse metallothionein-I (mMT-I) regulatory sequences to direct expression of human ornithine transcarbamylase in the liver of transgenic animals, fusion genes joining either 1.6 kilobases or 185 base pairs of the mMT-I regulatory region to the human ornithine transcarbamylase protein-coding sequence were used to produce transgenic mice. In mice carrying the fusion gene with 1.6 kilobases of the mMT-I 5'-flanking sequences, transgene expression was observed in a wide range of tissues, but, unexpectedly, expression in liver was never observed. Surprisingly, in mice carrying the fusion gene regulated by only 185 base pairs of the mMT-I 5'-flanking sequences, the transgene was expressed exclusively in male germ cells during the tetraploid, pachytene stage of meiosis.
authors.affiliation: Department of Human Genetics, Yale University School of Medicine, New Haven, Connecticut.
authors.forename: K A
authors.initials: KA
authors.lastname: Kelley
authors.forename: J W
authors.initials: JW
authors.lastname: Chamberlain
authors.forename: J A
authors.initials: JA
authors.lastname: Nolan
authors.forename: A L
authors.initials: AL
authors.lastname: Horwich
authors.forename: F
authors.initials: F
authors.lastname: Kalousek
authors.forename: J
authors.initials: J
authors.lastname: Eisenstadt
authors.forename: K
authors.initials: K
authors.lastname: Herrup
authors.forename: L E
authors.initials: LE
authors.lastname: Rosenberg
mesh_terms: D000818:Animals; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D008099:Liver / Q000201:enzymology; D008297:Male; D008540:Meiosis; D051379:Mice; D008822:Mice, Transgenic; D009693:Nucleic Acid Hybridization; D009954:Ornithine Carbamoyltransferase / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D013737:Testis / Q000166:cytology / Q000201:enzymology*
chemical_list: D009954:Ornithine Carbamoyltransferase; D004262:DNA Restriction Enzymes