Humoral and cellular immunity to varicella-zoster virus glycoprotein gpI and to a non-glycosylated protein, p170, in the strain 2 guinea-pig.
Strain 2 guinea-pigs were inoculated with infectious varicella-zoster virus (VZV) or with immunoaffinity-purified proteins of VZV. Monoclonal antibodies to the VZV gpI (90,000/58,000 complex) and to a non-glycosylated protein, p170, were used to prepare the polypeptide antigens. Humoral and cell-mediated immune responses to the infectious virus were compared with those elicited by the gpI and p170 proteins. Both VZV IgG antibody production and T lymphocyte proliferation to VZV were detected after immunization with infectious VZV and with VZV proteins. The antibody and T lymphocyte responses waned after protein immunization in comparison with the responses induced by infectious VZV but were detected again immediately after reimmunization with gpI or p170.
Arvin
A M
AM
Department of Pediatrics, Stanford University School of Medicine, California 94305.
Solem
S M
SM
Koropchak
C M
CM
Kinney-Thomas
E
E
Paryani
S G
SG
D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D006168:Guinea Pigs; D014645:Herpesvirus 3, Human / Q000276:immunology*; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000032:analysis; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D014764:Viral Proteins / Q000008:administration & dosage / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D014764:Viral Proteins