Influence of H-2K transfection on susceptibility of fibrosarcoma tumor cells to natural killer (NK) cells.
In this study, we have used the T-10 fibrosarcoma tumor cells to further analyze the relationship between metastatic competence, expression of H-2K antigens and susceptibility to lysis by virus augmented NK cells in vitro. Our results show an inverse correlation between metastatic properties of the original T-10 clones, IC9 and IE7, and susceptibility in vitro to lysis by virus-augmented NK cells. Restoration by transfection of expression of H-2K genes (H-2Kb or H-2Kk) led to the alteration of the metastatic phenotype of the tumors cells, yet had minor influence on the putative susceptibility of these clones to NK. These observations suggest that expression of MHC gene products, while affecting metastases, does not exclusively determine sensitivity to NK cells.
Gopas
J
J
Department of Microbiology and Immunology, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.
Segal
S
S
Hammerling
G
G
Bar-Eli
M
M
Rager-Zisman
B
B
D000818:Animals; D003587:Cytomegalovirus / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005354:Fibrosarcoma / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D009362:Neoplasm Metastasis; D013154:Spleen / Q000276:immunology; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000276:immunology*
D006183:H-2 Antigens