Mini-exon gene repeats of Trypanosoma (Nannomonas) congolense have internal repeats of 190 base pairs.
Genomic DNA hybridizations reveal that the mini-exon gene repeats of Trypanosoma (Nannomonas) congolense contain from one to greater than 20 internal tandem repeats of 190 base pairs (bp). The sequences of two mini-exon gene repeats were determined, one with three internal 190 bp repeats and one with a single 190 bp sequence. These internal repeats appear just after a very dC/dT-rich region, in the non-transcribed spacer regions between adjacent mini-exon transcription units. They do not occur in the genomes of the three other Trypanosoma species nor in the mini-exon gene repeats of four other kinetoplastids previously investigated. The mini-exon coding region of T. congolense differs in four positions from the T. brucei mini-exon sequence and in one position from a consensus sequence of the mini-exons found in other trypanosomatids. Pulse field gradient gels show that the mini-exon gene repeats of T. congolense are on large, greater than 2 megabase pair, chromosome(s).
Cook
G A
GA
Department of Biochemistry, University of Iowa, Iowa City 52242-1193.
Donelson
J E
JE
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D005091:Exons; D005796:Genes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014348:Trypanosoma congolense / Q000235:genetics*
D004247:DNA; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific