Heat shock-related decline in activity and amounts of active elongation factor 1 alpha in ageing and immortal human cells.
A significant decline in amount of active elongation factor, EF-1 alpha, and in its catalytic activity was observed in cell-free extracts prepared from normal human diploid fibroblasts (MRC-5) and their SV40-transformed counterparts, after subjecting the cells to 60 min heat shock at different temperatures. Old MRC-5 cells which had become senescent on serial passaging were more sensitive to heat shock-related changes in activity and amounts of active EF-1 alpha than were rapidly proliferating normal and transformed cells.
Rattan
S I
SI
Department of Chemistry, Aarhus University, Denmark.
Cavallius
J
J
Clark
B F
BF
D002460:Cell Line; D002461:Cell Line, Transformed; D002474:Cell-Free System; D046508:Culture Techniques / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000378:metabolism*; D013539:Simian virus 40 / Q000235:genetics
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors