ATP-gamma-S (adenosine 5'-O-(3-thiotriphosphate)) microinjection increases progesterone-stimulated histone kinase activity in Xenopus oocytes.
Progesterone stimulates the activity of a potent Ca2+- and cyclic nucleotide-independent histone kinase in Xenopus oocytes. Microinjection of ATP-gamma-S during the course of meiotic maturation or after the oocytes had arrested at second meiotic metaphase resulted in a further increase of this histone kinase activity in progesterone-stimulated oocytes. ATP-gamma-S microinjection also increased and stabilized the activity of the maturation-promoting factor. The possible mechanism of ATP-gamma-S action is discussed.
Labbé
J C
JC
INSERM U.249 and CNRS-CRBM, Montpellier, France.
Picard
A
A
Dorée
M
M
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000345:Affinity Labels / Q000494:pharmacology*; D000818:Animals; D004789:Enzyme Activation; D005260:Female; D006133:Growth Substances / Q000378:metabolism*; D016200:Maturation-Promoting Factor; D008845:Microinjections; D009865:Oocytes / Q000201:enzymology*; D010766:Phosphorylation; D011374:Progesterone / Q000494:pharmacology*; D006656:Protamine Kinase / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D014981:Xenopus
D000345:Affinity Labels; D006133:Growth Substances; C022571:adenosine 5'-O-(3-thiotriphosphate); D011374:Progesterone; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D006656:Protamine Kinase; D016200:Maturation-Promoting Factor