Photoaffinity labeling of the beta-adrenergic receptor in synaptic membranes of rat cerebral cortex and cerebellum.
The beta-adrenergic receptor polypeptides in synaptic membranes of rat cerebral cortex and cerebellum have been identified using the photoaffinity label [125I]iodoazidobenzylpindolol. The major receptor polypeptides possess apparent molecular weights of 62,000 and 49,000 (cerebral cortex) or 59,000 and 46,000 (cerebellum). Treatment of the membranes with endoglycosidase F caused the receptor polypeptides from both tissues to exhibit lower apparent molecular weight (51,000) on sodium dodecyl sulfate polyacrylamide gels, indicating that beta-adrenergic receptors in mammalian brain are glycoproteins as are the receptors in peripheral tissues.
Lautens
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LL
Department of Pharmacology, University of Wisconsin School of Medicine, Madison.
Ruoho
A E
AE
D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D001386:Azides / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000302:isolation & purification / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism*
D000345:Affinity Labels; D001386:Azides; D011943:Receptors, Adrenergic, beta; C029987:1-(indol-4-yloxy)-3-(1-(4-azido-3-iodophenyl)-2-isobutylamine)-2-propanol; D010869:Pindolol