Characterization of human DNA sequences synthesized at the onset of S-phase.
We developed a method of enrichment for DNA replicated at the onset of S-phase in synchronized human HL60 cells. About 200 such sequences were cloned. The analysis of this selected DNA sample showed that: 1) the cloned DNA fragments derive from a limited number (750-1500) of replicons; 2) there is no extensive homology between different DNA fragments; 3) they are not significantly enriched in highly repeated sequences; 4) they are enriched in snap-back (Cot = o) DNA. The sequence of the longest fragment revealed the presence of numerous signals collected in a few hundred nucleotides: 1) homology with the origin of replication of human Papovaviruses usually associated with potential stem-loop structures; 2) binding sites for known transcription factors and for another nuclear factor; 3) potential binding sites for the chromosome "scaffold".
Tribioli
C
C
Istituto di Genetica Biochimica ed Evoluzionistica del CNR, Pavia, Italy.
Biamonti
G
G
Giacca
M
M
Colonna
M
M
Riva
S
S
Falaschi
A
A
D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D007399:Interphase; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D004262:DNA Restriction Enzymes