By-products in the analysis of beta-muricholic acid in biological samples as methyl ester triacetate.
By-products were formed on analysis of beta-muricholic acid (3 alpha, 6 beta, 7 beta-trihydroxy-5 beta-cholan-24-oic acid) in biological samples by a method involving acid-catalyzed solvolysis of sulfate esters in acetone-methanol, followed by perchloric acid-catalyzed acetylation with acetic anhydride-acetic acid. These products have been identified by mass spectrometry and nuclear magnetic resonance as methyl 3-0,6-0-diacetyl-7-0-(1-methyl-3-oxo-1-butenyl)- and methyl 3-0,7-0-diacetyl-6-0-(1-methyl-3-oxo-1-butenyl)-beta-muricholate, methyl 3-0, 6-0-diacetyl- and methyl 3-0, 7-0-diacetyl-beta-muricholate, and a methyl diacetoxy-cholen-24-oate.
Janssen
G
G
Rega Institute, University of Leuven, Belgium.
Toppet
S
S
Compernolle
F
F
Parmentier
G
G
D000107:Acetylation; D001647:Bile Acids and Salts / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002793:Cholic Acids / Q000032:analysis*; D002849:Chromatography, Gas; D003641:Deamination; D004798:Enzymes / Q000378:metabolism; D004951:Esterification; D008279:Magnetic Resonance Imaging; D013058:Mass Spectrometry; D015394:Molecular Structure
D001647:Bile Acids and Salts; D002793:Cholic Acids; D004798:Enzymes; C004821:muricholic acid