title: Biochemical analysis of inhibitory effects of a lymphokine suppressive B-cell factor on the activation process of resting B cells.
abstract: Suppressive B-cell factor (SBF) is elaborated by FcR gamma (Fc receptor for IgG)-bearing small, resting B cells after the stimulation of immune complexes and is known to inhibit humoral immune responses by acting on resting B cells. In order to elucidate where and how SBF interferes with B-cell activation in the course of transmembrane signaling, we examined the effect of SBF on the several sequential events which B cells undergo after crosslinking surface immunoglobulin (sIg). Hyper-Ia expression, plasma membrane depolarization, and activation of phosphatidylinositol (PI) hydrolysis of resting B cells, all of which were induced by the stimulation with anti-mu antibody, were significantly suppressed by the pretreatment of cells with SBF. However, SBF had no effect on the intracytoplasmic cyclic AMP level of either activated or resting B cells. Another inhibitory effect of SBF on the activation process of resting B cells by anti-mu antibody was to suppress the transient elevation of intracytoplasmic free Ca2+ only in the initial phase after triggering with anti-mu antibody. This seems to be due to a decrease in the release of inositol triphosphate into the cytoplasm by suppressing the activation of PI hydrolysis. Considering all the data, the suppressive effect of SBF on the transmembrane signaling by sIg crosslinking is ascribed to the selective suppression of the activation of PI hydrolysis. This provides a concept on a molecular basis that feedback regulation of humoral immune response is, at least partly, regulated by SBF.
authors.affiliation: Institute for Immunology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohno
authors.forename: M
authors.initials: M
authors.lastname: Miyama-Inaba
authors.forename: K
authors.initials: K
authors.lastname: Tsjimura
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: R
authors.initials: R
authors.lastname: Kannagi
authors.forename: T
authors.initials: T
authors.lastname: Kanoh
authors.forename: H
authors.initials: H
authors.lastname: Uchino
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006868:Hydrolysis; D007166:Immunosuppressive Agents / Q000502:physiology*; D007399:Interphase / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010716:Phosphatidylinositols
chemical_list: D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D008222:Lymphokines; D010716:Phosphatidylinositols; C048134:suppressive B cell factor; D000242:Cyclic AMP