Control of thyroglobulin gene transcription by TSH and cAMP.
The availability of the sequence of Tg promoter from three different species enabled a fruitful comparison to be made from which a general picture of the organization of the Tg gene promoter region emerged. Chromatin structure studies identified tissue-specific modifications at the level of DNA protein interactions. A DNA element has been shown to be involved in transcription control by cAMP. This sequence includes a highly conserved region of the Tg gene promoter, overlaps a domain of the promoter which becomes hypersensitive to DNase I when the gene is expressed and displays specific protein-DNA interaction. These observations are consistent with the possibility that this sequence is a target element for trans-acting factor(s) which control(s) the transcriptional activity of the gene.
Christophe
D
D
Institut de Recherche Interdisciplinaire-(IRIBHN), Free University of Brussels, School of Medicine, Campus Erasme, Belgium.
Hansen
C
C
Gérard
C
C
Juvénal
G
G
Roger
P
P
Vassart
G
G
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002843:Chromatin / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004285:Dogs; D004347:Drug Interactions; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013954:Thyroglobulin / Q000096:biosynthesis* / Q000235:genetics; D013972:Thyrotropin / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D002843:Chromatin; D011993:Recombinant Fusion Proteins; D004815:Epidermal Growth Factor; D013972:Thyrotropin; D013954:Thyroglobulin; D000242:Cyclic AMP; D003850:Deoxyribonuclease I