Effect of adrenalectomy on diazinon-induced changes in carbohydrate metabolism.
Treatment with diazinon resulted in hyperglycaemia and depletion of glycogen from cerebral and peripheral tissues 2 h after its administration in rats; the changes were maximal after 40 mg/kg diazinon, administered intraperitoneally. The activities of glycogen phosphorylase and phosphoglucomutase were significantly increased in brain and liver, while that of glucose-6-phosphatase was not altered. The activities of the glycolytic enzymes hexokinase and lactate dehydrogenase were increased only in brain. The cholinesterase activity of the brain was reduced by treatment with diazinon. The activities of hepatic gluconeogenic enzymes (fructose 1,6 diphosphatase and phosphoenolpyruvate carboxykinase) were also significantly increased in diazinon-treated animals. The level of lactate was increased in brain and blood while that of pyruvate was not changed. The activity of glucose-6-phosphate dehydrogenase was not significantly changed. Cholesterol and ascorbic acid contents of adrenals were depleted in diazinon-treated animals. Adrenalectomy abolished the hyperglycaemia and changes in carbohydrate metabolism, suggesting the possible involvement of adrenals in the induced changes in diazinon-treated animals.
Matin
M A
MA
Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow, India.
Khan
S N
SN
Hussain
K
K
Sattar
S
S
D000315:Adrenalectomy; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001786:Blood Glucose / Q000378:metabolism; D001923:Brain Chemistry; D050260:Carbohydrate Metabolism; D002802:Cholinesterases / Q000097:blood / Q000378:metabolism; D003976:Diazinon / Q000633:toxicity*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007306:Insecticides; D007773:Lactates / Q000097:blood; D008112:Liver Glycogen / Q000378:metabolism; D011773:Pyruvates / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D007306:Insecticides; D007773:Lactates; D008112:Liver Glycogen; D011773:Pyruvates; D005954:Glucosephosphate Dehydrogenase; D002802:Cholinesterases; D001205:Ascorbic Acid; D003976:Diazinon