title: Abolition of actin-bundling by phosphorylation of human erythrocyte protein 4.9.
abstract: Protein 4.9, first identified as a component of the human erythrocyte membrane skeleton, binds to and bundles actin filaments. Protein 4.9 is a substrate for various kinases, including a cyclic AMP(cAMP)-dependent one, in vivo and in vitro. We show here that phosphorylation of protein 4.9 by the catalytic subunit of cAMP-dependent protein kinase reversibly abolishes its actin-bundling activity, but phosphorylation by protein kinase C has no such effect. A quantitative immunoassay showed that human erythrocytes contain 43,000 trimers of protein 4.9 per cell, which is equivalent to one trimer for each actin oligomer in these red blood cells. As analogues of protein 4.9 have been identified together with analogues of other erythroid skeletal proteins in non-erythroid tissues of numerous vertebrates, phosphorylation and dephosphorylation of protein 4.9 may be the basis for a mechanism that regulates actin bundling in many cells.
authors.affiliation: Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
authors.forename: A
authors.initials: A
authors.lastname: Husain-Chishti
authors.forename: A
authors.initials: A
authors.lastname: Levin
authors.forename: D
authors.initials: D
authors.lastname: Branton
mesh_terms: D000199:Actins / Q000097:blood* / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D046911:Macromolecular Substances; D008840:Microfilament Proteins; D010750:Phosphoproteins; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism
chemical_list: D000199:Actins; D001798:Blood Proteins; C044805:DMTN protein, human; D046911:Macromolecular Substances; D008840:Microfilament Proteins; D010750:Phosphoproteins; D000242:Cyclic AMP; D011494:Protein Kinases; D011493:Protein Kinase C