title: Evaluation of determination of uric acid in serum and whole blood with the Reflotron.
abstract: The performance of the Reflotron system (Boehringer Mannheim) for the determination of urate in whole blood and serum was evaluated. Within-run and day-to-day imprecision of the system were comparable with those for a solution-chemistry enzymatic method (overall CVs in the range 2.2-2.5%). Results for 100 individual specimens with urate concentrations ranging from 16 to 134 mg/L agreed well with the comparison method, both for serum and whole blood. We saw no significant interference from lipemia or hemoglobin. Bilirubin interfered at concentrations greater than 100 mg/L. Hematocrit variation between 25% and 55% did not affect results for whole blood; variation of the applied sample volume from 28 microL to 35 microL (stated sample volume requirement: 30 microL) did not significantly influence the measured value. We consider results produced by the system to be of the same analytical quality as those obtained by the more conventional solution-chemistry methods that are currently available.
authors.affiliation: Ospedale del Ponte, Varese, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Cattozzo
authors.forename: C
authors.initials: C
authors.lastname: Franzini
authors.forename: A
authors.initials: A
authors.lastname: Hubbuch
authors.forename: W
authors.initials: W
authors.lastname: Tritschler
mesh_terms: D001663:Bilirubin / Q000097:blood; D002784:Cholesterol / Q000097:blood; D005189:False Positive Reactions; D006400:Hematocrit; D006801:Humans; D009195:Peroxidase; D011786:Quality Control; D013223:Statistics as Topic; D014280:Triglycerides / Q000097:blood; D014503:Urate Oxidase; D014527:Uric Acid / Q000097:blood*
chemical_list: D014280:Triglycerides; D014527:Uric Acid; D002784:Cholesterol; D009195:Peroxidase; D014503:Urate Oxidase; D001663:Bilirubin