Partial purification and properties of lysosomes from rabbit testes.
Rabbit testes lysosomes were prepared by differential centrifugation and density gradient centrifugation on Percoll. The particulate material sedimented at 1.054 g/cm3. The enrichment in the activities of lysosomal marker enzymes was 9-fold. The contamination by other subcellular organelles such as mitochondria, microsomes and the plasma membrane was limited to 0.1% or less. The marker enzymes of isolated lysosomes exhibited bound activity of 80%, thus indicating the integrity of granules. The testicular lysosomes also exhibited osmofragility characteristics.
Ninjoor
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Department of Biochemistry, University of Georgia, Athens.
Srivastava
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PN
D015720:5'-Nucleotidase; D000118:Acetylglucosaminidase / Q000032:analysis; D000818:Animals; D002498:Centrifugation; D002499:Centrifugation, Density Gradient; D003576:Electron Transport Complex IV / Q000032:analysis; D005952:Glucose-6-Phosphatase / Q000032:analysis; D008247:Lysosomes / Q000032:analysis* / Q000201:enzymology; D008297:Male; D009708:Nucleotidases / Q000032:analysis; D009996:Osmotic Fragility; D011817:Rabbits; D013347:Subcellular Fractions / Q000201:enzymology; D013737:Testis / Q000032:analysis* / Q000201:enzymology
D003576:Electron Transport Complex IV; D009708:Nucleotidases; D015720:5'-Nucleotidase; D005952:Glucose-6-Phosphatase; D000118:Acetylglucosaminidase