Involvement of the Na+/H+ antiporter in activation of rat platelets by collagen.
The rapid Ca2+ increase shown by quin2-loaded platelets was found to be an artifact, probably due to light scattering elicited by collagen. Further findings as to fura2-loaded platelets offered additional support, demonstrating that the initial activation of phospholipase A2 (PLA2) does not require cytoplasmic Ca2+ mobilization. A possible role of the Na+/H+ antiporter as a trigger for collagen-induced activation of PLA2 in rat platelets was presented for the first time.
Nakano
T
T
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka.
Hanasaki
K
K
Arita
H
H
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000502:physiology*; D003094:Collagen / Q000494:pharmacology*; D004789:Enzyme Activation; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D051381:Rats; D017923:Sodium-Hydrogen Exchangers
D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D003094:Collagen; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium