Anti-idiotypic antibodies raised against anti-metenkephalin antibodies in rabbits.
Anti-idiotypic antibodies were raised in rabbits immunized against immunoglobulins purified from anti-metenkephalin antibody. The antibodies competed dose-dependently with metenkephalin in binding to anti-metenkephalin antibody on a solid phase metenkephalin enzyme-linked immunosorbent assay (ELISA). The titers of the antiidiotypic activity appeared characteristically in transient peaks and troughs in alternation with anti-metenkephalin activities and were retained after purification on various affinity columns.
Jen
M F
MF
Department of Pharmacology, Chinese Academy of Medical Sciences, Beijing, China.
Liu
Y X
YX
Chin
Y C
YC
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D004744:Enkephalin, Methionine / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D011817:Rabbits / Q000276:immunology; D011957:Receptors, Opioid / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D011957:Receptors, Opioid; D004744:Enkephalin, Methionine