[Weakening of type I restriction in E. coli: the effect of dam mutation].
The host-controlled EcoK-restriction of unmodified phages lambda.0 and T7ocr. is 100-fold alleviated in dam- mutants of E. coli. In addition the EcoK modification activity is considerably decreased in dam- strains. The I and III types restriction (EcoB, EcoD, EcoK and EcoP1) were relieved in dam- mutants, but no alleviation of EcoRI restriction occurred in dam- strains. We interpret the alleviation of the I type restriction in dam- mutants as consequence of induction of the function, which interferes with the I type restriction systems.
Belogurov
A A
AA
Efimova
E P
EP
Del'ver
E P
EP
Zavil'gel'skiĭ
G B
GB
D010582:Bacteriophage lambda / Q000235:genetics; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D009154:Mutation
D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes
Oslablenie restriktsii I tipa u E. coli: deĭstvie mutatsii dam.