[The protein-synthesizing apparatus of a BHK-21 (C-13) cell culture and of its large-cell clone C-9].
Cell line BHK-21 (C-13) and its large cell clone C-9 differ in morphology, karyotype and cultural properties. Clone C-9 is polyploid. It has been shown that C-9 clone cells display 2.5-3-fold excess in the nucleolus organizer region (NOR) in chromosomes, and 2-3 times higher intensity of protein synthesis and of ribosomal material content compared to the original line. Data of sedimentation analysis and of protein synthesis activity of the total ribosomal material in the cell-free translational system from rabbit reticulocytes allow to conclude that the quantity and size of polyribosomes in C-9 cells are higher than in cells of the original line. Apparently, the quantity of NOR-chromosomes reflect the activity of protein translation system of investigated cells.
Iamshchikova
G V
GV
Urmanova
M A
MA
Balzovskaia
E G
EG
Riabchikova
E I
EI
Beklemishev
A B
AB
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells / Q000378:metabolism / Q000648:ultrastructure; D007621:Karyotyping; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009697:Nucleolus Organizer Region / Q000378:metabolism / Q000648:ultrastructure; D014176:Protein Biosynthesis; D012260:Ribonucleases / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure
D012260:Ribonucleases
Issledovanie beloksinteziruiushchego apparata kul'tury kletok VNK-21 (C-13) i ee krupnokletochnogo klona C-9.