title: Loss of pathogenicity of spleen focus-forming virus after pseudotyping with Akv.
abstract: Friend virus complex (FV), which comprises replication-competent Friend murine leukemia virus (FMuLV) plus replication-defective spleen focus-forming virus (SFFV), induces a multistage erythroleukemia. We have examined the role of replication-competent helper virus in the early and late stages of FV disease by replacing FMuLV, the native helper, with Akv, the endogenous ecotropic MuLV of AKR mice. SFFVP/FRE, an established fibroblast line nonproductively infected with the polycythemic strain of SFFV, was superinfected with FMuLV or with Akv. Although supernatants from these cells showed similar titers in the XC plaque assay, supernatants from Akv-infected SFFVP/FRE cells showed 100- to 5,000-fold less activity than did those from FMuLV-infected cells with respect to spleen focus induction in vivo. Since virions isolated from these two supernatants contained similar ratios of SFFV to helper virus genomic RNA, it did not appear that the difference was due to a relative inability of Akv to package SFFV. Although FMuLV- and Akv-rescued SFFV are equally infectious in a mouse fibroblast cell line (NIH 3T3), FMuLV-rescued SFFV was far more efficient in inducing erythroid bursts in cultured primary bone marrow cells. Adding Akv to preparations of FMuLV-rescued SFFV did not significantly interfere with burst induction. Helper-free SFFV induced 50- to 500-fold more spleen foci when coinjected with FMuLV than it did with Akv. Helper virus also affected mortality rates that reflect the late stage of the disease. When FMuLV- or Akv-rescued SFFV was injected into NIH Swiss mice at dosage levels adjusted to give equal numbers of spleen foci, all mice receiving FMuLV-rescued SFFV developed splenomegaly and died, whereas no mice receiving Akv-rescued SFFV died or developed detectable splenomegaly. When FMuLV was coinjected with Akv-rescued SFFV, the mortality rate rose from 0 to 100%. Injection of helper-free SFFV alone did not induce mortality, but coinjection of helper-free SFFV with FMuLV resulted in 100% mortality. Thus, the helper virus used to rescue SFFV plays at least a quantitatively important role in the early stage of FV disease and a crucial role in the late stage of the disease in vivo.
authors.affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: K S
authors.initials: KS
authors.lastname: Jones
authors.forename: S
authors.initials: S
authors.lastname: Ruscetti
authors.forename: F
authors.initials: F
authors.lastname: Lilly
mesh_terms: D000406:AKR murine leukemia virus / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005622:Friend murine leukemia virus / Q000502:physiology; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000472:pathogenicity* / Q000502:physiology*; D004915:Leukemia, Erythroblastic, Acute / Q000382:microbiology*; D008297:Male; D051379:Mice; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000235:genetics; D013155:Spleen Focus-Forming Viruses / Q000235:genetics / Q000472:pathogenicity*
chemical_list: D012367:RNA, Viral