In situ nick translation distinguishes between C-band positive regions on mouse chromosomes.
In situ nick translation of mouse metaphase chromosomes by non-radioactive detection means and DNase I digestion followed by Giemsa staining were used to analyse the DNase I resistance of two different C-band positive regions. These were the centromeric heterochromatin of acro- and metacentric chromosomes and an interstitial C-band on chromosome 1 of wild mice, IS(HSR;1C5D)1Lub. Whereas the centromeric heterochromatin was clearly resistant to DNase I, the interstitial C-band showed very high DNase I sensitivity. Among centromeric C-bands, the heterochromatin in Robertsonian fusion biarmed chromosomes was more resistant to DNase I action than was the centromeric heterochromatin of the acrocentric chromosomes.
Adolph
S
S
Abteilung Klinische Genetik, Universität Ulm, Ulm/Donau, Federal Republic of Germany.
D000818:Animals; D001710:Biotin; D002503:Centromere / Q000187:drug effects / Q000648:ultrastructure; D002871:Chromosome Banding / Q000379:methods*; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D003850:Deoxyribonuclease I / Q000494:pharmacology; D003856:Deoxyuracil Nucleotides; D006570:Heterochromatin / Q000187:drug effects / Q000648:ultrastructure; D008677:Metaphase; D051379:Mice; D013942:Thymine Nucleotides; D014178:Translocation, Genetic; D014316:Tritium
D003856:Deoxyuracil Nucleotides; D006570:Heterochromatin; D013942:Thymine Nucleotides; D014316:Tritium; C027078:deoxyuridine triphosphate; D001710:Biotin; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; C024157:thymidine 5'-triphosphate