The bovine leukemia virus p34 is a transactivator protein.
Recombinant Moloney murine retroviruses containing the BLV post-envelope long open reading frame were constructed and transfected into the psi 2 packaging cell line. They were shown to encode and to express a 34-kd protein able to transactivate the BLV long terminal repeat-directed gene expression in the respective transfected cells. These data demonstrate that the BLV X-LOR gene encodes a p34 transactivator product. Furthermore, the different cell lines produced infectious recombinant retroviruses capable of transferring X-LOR genes into recipient cells. The availability of the BLV transactivator protein should allow us to understand the role of the transactivator protein in BLV-induced leukemogenesis.
Willems
L
L
Faculty of Agronomy, Gembloux, France.
Gegonne
A
A
Chen
G
G
Burny
A
A
Kettmann
R
R
Ghysdael
J
J
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000378:metabolism*
D014157:Transcription Factors; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D004247:DNA