Positive selection of antigen-specific B lymphocytes by means of immunomagnetic particles.
This paper presents a method for the isolation of highly enriched antigen-specific B lymphocytes from peripheral blood mononuclear cells (PBMC) by means of an immunomagnetic procedure. The method is illustrated by the isolation of B cells positive for rheumatoid factors (RF) from patients with rheumatoid arthritis and healthy blood donors. After Epstein-Barr virus transformation of isolated cells, more than 90% of the generated immunoglobulin-secreting cells produced RF in several experiments. Compared to the low frequency of B cells positive for RF in PBMC, this represents a 10(3)- to 10(4)-fold enrichment of antigen-specific B cells.
Egeland
T
T
Institute for Immunology and Rheumatology, Rikshospitalet University Hospital, Oslo, Norway.
Hovdenes
A
A
Lea
T
T
D001172:Arthritis, Rheumatoid / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007158:Immunologic Techniques; D008280:Magnetics; D012217:Rheumatoid Factor / Q000096:biosynthesis / Q000276:immunology*
D007136:Immunoglobulins; D012217:Rheumatoid Factor