Molecular analysis of G+C-rich upstream sequences regulating transcription of the human carbonic anhydrase II gene.
The upstream promoter sequences of the human carbonic anhydrase II (CA II) gene have been studied by 5' deletion analysis. Promoter activity was assayed by transfection and chloramphenicol acetyltransferase assay in both human HeLa cells and murine L cells. This investigation showed that the CA II promoter is comparable in activity to that of the simian virus 40 early-region promoter and enhancer and that the CA II upstream sequences exert a different pattern of control in the two cell lines.
Shapiro
L H
LH
Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109-0618.
Venta
P J
PJ
Tashian
R E
RE
D000818:Animals; D001482:Base Composition; D002256:Carbonic Anhydrases / Q000235:genetics*; D003062:Codon; D004274:DNA, Recombinant; D006367:HeLa Cells; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D003062:Codon; D004274:DNA, Recombinant; D002256:Carbonic Anhydrases