Rabbit M type phosphoglyceromutase: comparative effects of two thiol reagents antibody reaction and hybridization studies.
1. Treatment of purified rabbit phosphoglyceromutase (M type) with N-ethylmaleimide or with iodoacetamide produces the concurrent loss of phosphoglyceromutase activity with its collateral glycerate-2,3-P2 phosphatase activity. 2. Differences are observed in the protective effect of glycerate-2,3-P2 and of glycolate-2-P against N-ethylmaleimide and iodoacetamide treatments. 3. Specific chicken antibodies obtained by injection of the purified rabbit M type phosphoglyceromutase do not cross-react with the B type but neutralize both rabbit and human M type phosphoglyceromutase. 4. Purified rabbit M type phosphoglyceromutase can hybridize in vitro with the purified human B type or with purified human glycerate-2,3-P2 synthase. 5. Its ability to hybridize with glycerate-2,3-P2 synthase is unchanged after iodoacetamide treatment.
Prehu
M O
MO
Inserm U.91, Hôpital Henri Mondor, Crétil, France.
Prehu
C
C
Calvin
M C
MC
Rosa
R
R
D000818:Animals; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D005033:Ethylmaleimide / Q000494:pharmacology*; D006801:Humans; D005779:Immunodiffusion; D007460:Iodoacetamide / Q000494:pharmacology*; D007461:Iodoacetates / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism*; D010736:Phosphoglycerate Mutase / Q000276:immunology / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011817:Rabbits; D013045:Species Specificity
D007461:Iodoacetates; D007527:Isoenzymes; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase; D005033:Ethylmaleimide; D007460:Iodoacetamide