Chimeric protein: abrin B chain-trypsin inhibitor conjugate as a new antitumor agent.
Abrin B chain and trypsin inhibitor isolated from Acacia confusa (ACTI) were covalently linked to form a chimeric protein (ANB-ACTI) with N-succinimidyl-3-(-2-pyridyldithio)propionate. The chimeric protein had 31% of trypsin inhibitory activity of ACTI and 7% of hemagglutinating activity of abrin B chain, but no inhibition on protein biosynthesis. ANB-ACTI had strong inhibitory effects on the growth of sarcoma 180 cells and Hela cell culture while the mixture of an equivalent amount of free abrin B chain and ACTI did not. The results suggests that abrin B chain of chimeric protein may act as a vector to carry ACTI into the tumor cells. ACTI into the tumor cells. ACTI in the chimeric protein potentiates its antitumor activity as well as its resistance to tryptic digestion.
Lin
J Y
JY
Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, R.O.C.
Hsieh
Y S
YS
Chu
S C
SC
D000036:Abrin / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D002455:Cell Division / Q000187:drug effects; D003432:Cross-Linking Reagents; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D010940:Plant Proteins / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy; D013388:Succinimides; D014361:Trypsin Inhibitors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D003432:Cross-Linking Reagents; D010940:Plant Proteins; D013388:Succinimides; D014361:Trypsin Inhibitors; D000036:Abrin; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate