Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene.
A plasmid expressing the cloned bacteriophage T4 gene 42 gave the same levels of complementation of gene 42 mutants in a polarity-suppressing rho mutant as in a rho+ host. A reading frame likely corresponding to gene 42 and putative promoter and terminator sequences were identified in the partial sequence of the cloned fragment.
Thylén
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Department of Microbiology, Faculty of Pharmacy, Uppsala University, Sweden.
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005816:Genetic Complementation Test; D019877:Hydroxymethyl and Formyl Transferases; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic; D014166:Transferases / Q000235:genetics*
D004279:DNA, Viral; D014166:Transferases; D019877:Hydroxymethyl and Formyl Transferases; C100226:deoxycytidylate hydroxymethyltransferase; D004262:DNA Restriction Enzymes