Physical and functional map of an amikacin-resistance plasmid isolated from a multiresistant strain of Serratia marcescens.
pTK159, a multiresistance 40-kilobase (kb) plasmid, was isolated from a clinical strain of Serratia marcescens. pTK159 was nonconjugative and carried determinants for resistance to amikacin, streptomycin/spectinomycin, sulfamethoxazole and ampicillin. A physical and functional map of pTK159 was constructed. By cloning various fragments of pTK159 in pACYC184 or pBR322, genes for resistance to amikacin, streptomycin/spectinomycin, and sulfamethoxazole were found to be located on a 2.0-kb BamHI-HindIII fragment, a 1.4-kb HindIII fragment and a 2.1-kb HindIII fragment, respectively. The map of pTK159 was compared with published maps of amikacin-resistance determinants and transposons.
Kanno
R
R
Department of Bacteriology, Teikyo University School of Medicine, Itabashi-ku, Tokyo.
D000583:Amikacin / Q000494:pharmacology*; D004262:DNA Restriction Enzymes; D004352:Drug Resistance, Microbial / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D011815:R Factors; D012706:Serratia marcescens / Q000187:drug effects / Q000235:genetics*
D000583:Amikacin; D004262:DNA Restriction Enzymes