title: Map location of the thymidine kinase gene of bovine herpesvirus 1.
abstract: Bovine herpesvirus 1 has been reported to contain a thymidine kinase (tk) gene which is nonessential for virus replication. We have isolated a thymidine kinase-negative mutant of the virus and localized the mutation by marker rescue experiments to a 1.1-kilobase BglII-SalI fragment which maps at 0.47 to 0.48 on the bovine herpesvirus 1 genomic map. A thymidine kinase-negative bovine cell line isolated in our laboratory was used in these studies.
authors.affiliation: Laboratory of Microbiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6049.
authors.forename: L J
authors.initials: LJ
authors.lastname: Bello
authors.forename: J C
authors.initials: JC
authors.lastname: Whitbeck
authors.forename: W C
authors.initials: WC
authors.lastname: Lawrence
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D015247:Deoxyribonuclease HindIII; D005814:Genes, Viral; D005819:Genetic Markers; D007242:Herpesvirus 1, Bovine / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D013937:Thymidine Kinase / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D005819:Genetic Markers; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII