Refined X-ray structure of the low pH form of ribonuclease T1-2'-guanylic acid complex at 1.9 A resolution.
The three-dimensional X-ray structure of the RNase T1[EC 3.1.27.3]-2'GMP complex crystallized at low pH value (4.0) was determined, and refined to 1.9 A resolution to give a final R value of 0.203. The refined model includes 781 protein atoms, 24 inhibitor atoms, and 43 solvent molecules. The imidazole rings of His27 and His40 interact with the carboxyl side chains of Glu82 and Glu58, respectively, whereas that of His92 is in contact with the main chain carbonyl oxygen of Ala75. In the complex, the ribose ring of the 2'GMP molecule adopts a C2'-endo puckering, and the exocyclic conformation is gauche(-)-gauche(+). The glycosyl torsion angle is in the syn range with an intramolecular hydrogen bond between N3 and O5', and the 2'-phosphate orientation is trans-gauche(-). The guanine base of the inhibitor is tightly bound to the base recognition site with five hydrogen bonds (N1--Glu46O epsilon 2, N2---Asn98O,O6---Asn44N, and N7 ---Asn43N delta 2/Asn43N) and is sandwiched between the phenolic ring portions of Tyr42 and Tyr45 by stacking interactions. The 2'-phosphate group interacts with Arg77N eta 2, Glu58O episilon 2, and Tyr 38O eta but not with any of the histidine residues. Arg77N eta 2 also interacts with Tyr38O eta. There is no interaction between the ribose moiety of the inhibitor and the enzyme.
Sugio
S
S
Faculty of Pharmaceutical Sciences, Osaka University.
Amisaki
T
T
Ohishi
H
H
Tomita
K
K
D003460:Crystallization; D004722:Endoribonucleases / Q000032:analysis*; D006150:Guanine Nucleotides / Q000032:analysis*; D006157:Guanosine Monophosphate / Q000032:analysis*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008958:Models, Molecular; D006163:Ribonuclease T1 / Q000032:analysis*; D012997:Solvents; D014961:X-Ray Diffraction
D006150:Guanine Nucleotides; D046911:Macromolecular Substances; D012997:Solvents; C026693:2'-guanylic acid; D006157:Guanosine Monophosphate; D004722:Endoribonucleases; D006163:Ribonuclease T1