Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation.
The effects of the restriction system of Bacillus subtilis strain M on plasmid transformation were studied. Plasmid pHV1401 DNA prepared from B. subtilis transformed the restriction-proficient M strain 100 times more efficiently than the DNA prepared from Escherichia coli, while the two DNA preparations transformed restriction-deficient derivatives of that strain with similar efficiencies. This indicates that transformation with pHV1401 is sensitive to the M restriction system. pHV1401 contains three CTCGAG (XhoI sites). Successive removal of these abolished the effect of restriction. This indicates that the XhoI sites are the targets for the M restriction system.
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Department of Genetics, Center of Biological Sciences, Haren (Gn), The Netherlands.
Jannière
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Ehrlich
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SD
D001412:Bacillus subtilis / Q000235:genetics*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000502:physiology*; D004262:DNA Restriction Enzymes / Q000502:physiology*; D004269:DNA, Bacterial / Q000378:metabolism*; D005838:Genotype; D010957:Plasmids; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes