Desensitization of immature rat testicular ornithine decarboxylase to arginine vasopressin.
Prior exposure of immature rat testis to arginine vasopressin caused the testis refractory at 24 h in terms of ornithine decarboxylase activity. Arginine vasopressin caused desensitization both in Leydig cells and seminiferous tubules. Arginine vasopressin induced desensitization was found to be both time and dose-dependent. Arginine vasopressin desensitized testis was refractory to luteinizing hormone, follicle stimulating hormone, norepinephrine, dibutyryl cAMP, phorbol-myristate acetate and cholera toxin at 24 h. Arginine vasopressin desensitized testis showed recovery of response to norepinephrine at 48 h after the first injection. On the contrary arginine vasopressin could stimulate ornithine decarboxylase in luteinizing hormone desensitized testis. These results indicate that in arginine vasopressin desensitized testis the block is at post cAMP step which is common to both cAMP dependent and protein kinase C-diacylglycerol system in stimulating testicular ornithine decarboxylase.
Reddy
B V
BV
School of Life Sciences, University of Hyderabad, India.
Madhubala
R
R
Reddy
P R
PR
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007700:Kinetics; D007985:Leydig Cells / Q000201:enzymology; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012671:Seminiferous Tubules / Q000201:enzymology; D012741:Sexual Maturation; D013737:Testis / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D001127:Arginine Vasopressin; D003994:Bucladesine; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate; D009638:Norepinephrine