title: Clonal analysis of radiation leukemia virus-induced leukemic and preleukemic murine cells.
abstract: Clonality of radiation leukemia virus (RadLV)-induced thymic lymphomas was determined by detection of rearrangements in the genetic locus coding for the beta chain of the T-cell receptor (T beta). Unique T beta rearrangements were detected in four of six lymphomas. Two of the T beta-rearranged thymic lymphomas and two in which such a rearrangement was not detected had a biallelic deletion of C beta 1. With an anti-RadLV monoclonal antibody it was found that 1-2 days after virus inoculation more than one-third of the cells in the thymus were infected by the virus. The frequency of virus-positive cells gradually declined and persisted at 1-2% until the appearance of a clonal lymphoma at which time virtually all the cells in the thymus were virus positive. Transfer of thymocytes from a single, preleukemic mouse 21 days post-virus inoculation into several adoptive recipients resulted in donor-type thymic lymphomas in the majority of the mice. T beta rearrangement analysis revealed that these lymphomas were clonal and derived from different potentially leukemic (preleukemic) cells in the thymus of the donor mouse. Eleven of 15 lymphomas had a biallelic deletion of C beta 1. These results suggest that clonal, RadLV-induced thymomas are selected from an oligoclonal, RadLV-infected preleukemic T-cell population.
authors.affiliation: The Lautenberg Center for General and Tumor Immunology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Ben-David
authors.forename: E
authors.initials: E
authors.lastname: Yefenof
authors.forename: M
authors.initials: M
authors.lastname: Kotler
mesh_terms: D000483:Alleles; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis; D002872:Chromosome Deletion; D002999:Clone Cells / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D007942:Leukemia, Experimental / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011289:Preleukemia / Q000473:pathology*; D012190:Retroviridae / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000648:ultrastructure; D014412:Tumor Virus Infections / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific