Evaluation of laboratory-prepared high-performance size-exclusion columns. Applications to food analysis.
This paper describes an inexpensive and relatively simple procedure to prepare high-performance size-exclusion columns from laboratory-packed or commercially pre-packed high-performance liquid chromatography columns by chemical deactivation of the surface of the silica packing material. The columns can be prepared quickly and reproducibly, they are chromatographically as efficient as high-performance size-exclusion columns commercially available, and are prepared at a fraction of the cost. Examples of the separation of proteins, peptides and polysaccharides on laboratory-prepared columns with differing permeation characteristics are illustrated and discussed.
Lumley
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ID
Laboratory of the Government Chemist, Cornwall House, London, U.K.
Patel
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I
Cohen
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IC
D002611:Cheese / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004044:Dietary Proteins / Q000302:isolation & purification; D004527:Egg Proteins / Q000032:analysis; D005504:Food Analysis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D004044:Dietary Proteins; D004527:Egg Proteins; D007202:Indicators and Reagents; D012822:Silicon Dioxide