[Genetic hypertension in the SHR rat and circulating digitalis compounds].
Circulating digitalis-like compounds have been found elevated in some experimental sodium--and volume--dependent hypertensions, as well as in human essential hypertension. As few studies have been undertaken to assess their enhancement in the genetic hypertension of Okamoto (SHR) we have investigated their presence in plasma using 4 criteria: their apparent immunoreactivity with antidigoxin antibodies, their competition with tritiated ouabain binding to the sodium pump of human red blood cells,-their ability to inhibit the Na+, K+ ATPase activity of rat kidney membranes, and the Na+ fluxes from rat red blood cells. When compared to ordinary Wistar (W) and Wistar Kyoto rats (WKY), SHR exhibited a markedly enhanced apparent immunoreactivity with antidigoxin-antibodies (138 +/- 8; 59 +/- 3; 61 +/- 4 pg/ml, n = 15, 6 et 15, p less than 0.001, and p less than 0.001 respectively). The inhibition of ouabain binding by plasma extracts of the three strains did not differ (10.3 +/- 1.6, 9.9 +/- 1.7 and 12.9 +/- 1.4 ng/ml, n = 9, 18 and 14 respectively). When compared to WKY, SHR plasma extracts inhibited the renal Na+, K+ ATPase activity (75.6 +/- 2.6 vs 89.3 +/- 2.4 mumoles Pi . mg-1 . h-1, n = 11 and 10, p less than 0.01, respectively). When incubated in SHR plasma for one hour, net sodium effluxes from Wistar erythrocytes were inhibited compared to that measured in the presence of W or WKY plasma: (5.91 +/- 0.20 vs 7.68 +/- 0.25 and 7.52 +/- 0.15 mmol/l cells, n = 5, 3 and 5, p less than 0.001, and p less than 0.001 respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
Wauquier
I
I
l'Unité INSERM n. 7, laboratoire associé CNRS n. 318. Hôpital Necker, Département de Pharmacologie, Paris.
Pernollet
M G
MG
Grichois
M L
ML
Meyer
P
P
Devynck
M A
MA
D000818:Animals; D001667:Binding, Competitive; D003429:Cross Reactions; D004071:Digitalis Glycosides / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism; D006973:Hypertension / Q000097:blood* / Q000235:genetics; D008297:Male; D010042:Ouabain / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR / Q000097:blood*; D011919:Rats, Inbred Strains / Q000097:blood*; D011921:Rats, Inbred WKY; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D004071:Digitalis Glycosides; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase
Hypertension génétique du rat SHR et composés "digitaliques" circulants.