title: Change in the amount of rRNA precursor synthesized in rat ventral prostates after administration of androgen and cycloheximide: application of quantitative S1 nuclease mapping.
abstract: The change in amount of pre-rRNA was observed by the use of total cellular RNA isolated from rat ventral prostates and quantitative S1 nuclease mapping. The amount of pre-rRNA from castrated rats was about 20% of that of normal rats, and increased to the normal level 24h after administration of testosterone. Cycloheximide caused a rapid decrease in the amount of pre-rRNA in normal and testosterone-treated castrated rats, to about 20% and 10% of that of normal rats, respectively, 2h after the administration of the drug. These results support the view that the androgen-stimulated initiation of the rRNA gene transcription is mediated by a hormone-dependent short-lived protein(s).
authors.affiliation: Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsui
authors.forename: S
authors.initials: S
authors.lastname: Horikawa
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Tsukada
authors.forename: T
authors.initials: T
authors.lastname: Hosoya
mesh_terms: D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D004720:Endonucleases; D005796:Genes / Q000187:drug effects; D007700:Kinetics; D008297:Male; D009710:Nucleotide Mapping; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D012322:RNA Precursors / Q000187:drug effects / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013739:Testosterone / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D012322:RNA Precursors; D013739:Testosterone; D003513:Cycloheximide; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases