[Synthesis and immunogenic properties of peptides corresponding to the 200-210 site of the oncoprotein p28sis].
Synthesis of peptides corresponding to the 201-210 and 200-206 sequences of p28sis oncoprotein is reported. This region of the oncoprotein was chosen as a probable epitope basing on the analysis of its secondary structure and hydrophilicity profile. The synthesis was carried out by classical method in solution using side-protecting groups of the benzyl type. Conjugates of the peptides with protein carriers and polyclonal antibodies to the peptides were obtained. Antiserum against the 201-210 peptide conjugate is shown to be specific to p28sis oncoprotein and its p56sis dimer.
Antonenko
V V
VV
Prudchenko
I A
IA
Mikhaleva
I I
II
Mazurenko
N N
NN
Kiselev
F L
FL
D000595:Amino Acid Sequence; D002471:Cell Transformation, Neoplastic; D020575:Oncogene Proteins v-sis; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology; D012191:Retroviridae Proteins / Q000235:genetics*; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics
D020575:Oncogene Proteins v-sis; D010455:Peptides; D012191:Retroviridae Proteins
Sintez i immunogennye svoĭstva peptidov uchastka 200-210 posledovatel'nosti onkobelka p28sis.