Induction and suppression of type I collagenase in cultured human cells.
A number of peptide growth factors have been shown to induce the secretion of type I collagenase into the medium of human fibroblast cultures (Chua et al., 1985). In this study the ability of eye-derived growth factor, lectin and tumor-promoting agents on collagenase induction in human fibroblast cells were examined. These agents were found to be able to induce collagenase production to a similar extent as epidermal growth factor. Dexamethasone at 10-100 nM was found to suppress collagenase induction in human fibroblast cells. The cell-type specificity of this enzyme induction by growth factors was studied by using a human epidermoid carcinoma cell line, A-431. An Mr 55,000 band appeared in the medium of A-431 cells upon 22 h exposure to EGF. Two-dimensional peptide pattern of the Mr 55,000 band in A-431 cells was identical to the counterpart in HF cells. Our results indicated that the induction of collagenase was not unique to human fibroblast cultures.
Chua
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Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
Barritault
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Geiman
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Ladda
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D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D003012:Microbial Collagenase / Q000096:biosynthesis*; D008970:Molecular Weight; D009799:Ocular Physiological Phenomena; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D006133:Growth Substances; C039825:eye-derived growth factor; D003208:Concanavalin A; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D003907:Dexamethasone; D003012:Microbial Collagenase; D013755:Tetradecanoylphorbol Acetate