Characterization of the state of differentiation of six newly established human non-small-cell lung cancer cell lines.
Six new non-small-cell lung cancer (NSCLC) cell lines were established directly from human tissue or indirectly via nude mouse xenografts in serum-supplemented media with success rates of 8% and 13%, respectively. They comprised one adenocarcinoma (ADLC-5M2), two squamous cell carcinomas (EPLC-32M1, EPLC-65H), two large cell carcinomas (LCLC-97TM1, LCLC-103H), and one malignant biphasic mesothelioma (MSTO-211H). All cell lines grew adherent to culture vessels with population doubling times (PDT) of 16-40 h, formed colonies in soft agarose with efficiencies of 0.1%-5.1%, and all grew in athymic nude mice. Xenograft histologies appeared as follows: (a) undifferentiated carcinomas with feeble resemblance to the original tumors in the case of adenocarcinomas and squamous cell carcinomas; (b) large cell carcinoma with high resemblance to the original tumor; (c) an undifferentiated tumor with predominance of large epithelial cells and few fibrous cells in the case of mesothelioma. Human chorionic gonadotropin (HCG) was found by radioimmunoassay and high-affinity binding sites for epidermal growth factor (EGF) by radio-receptor assay in 4/4 cell lines. A very low activity of L-DOPA decarboxylase (DDC) was detectable only in the adenocarcinoma cell line. All cell lines overexpressed the c-myc protooncogene, and no gene rearrangement or amplification was observed. Chromosome analysis revealed modal chromosome numbers of 70-73 in ADLC-5M2, EPLC-32M1, EPLC-65H, and MSTO-211H. Cell lines derived from large cell carcinoma had modal values of 65 and 170 and a wider chromosome distribution than all other cell lines. A NSCLC specific chromosomal aberration has been undetectable until now. These cell lines may aid in elucidating the biology of NSCLC and its interrelationship to other lung tumors.
Bepler
G
G
Philipps University Medical Center, Department of Internal Medicine, Marburg, Federal Republic of Germany.
Koehler
A
A
Kiefer
P
P
Havemann
K
K
Beisenherz
K
K
Jaques
G
G
Gropp
C
C
Haeder
M
M
D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000032:analysis / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D003470:Culture Media; D004273:DNA, Neoplasm / Q000032:analysis; D004296:Dopa Decarboxylase / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000032:analysis / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012334:RNA, Neoplasm / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000166:cytology*
D003470:Culture Media; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D004296:Dopa Decarboxylase