title: Ubiquitin in stressed chicken embryo fibroblasts.
abstract: Ubiquitin, a small 76-amino acid protein which is highly conserved in eukaryotic cells, occurs in several forms other than the free polypeptide. Among these are protein conjugates in which ubiquitin is covalently linked in lysylpeptide bond to lysl residues of other proteins and fusion proteins in which the amino-terminal domain is the precise ubiquitin sequence. Ubiquitin plays a role in cellular proteolytic degradation and in chromatin structure and has been postulated to be involved in the induction of a set of proteins which function during the cellular response to various kinds of environmental stress. We have measured the various forms of ubiquitin in cultures of chicken embryo fibroblasts under normal growth conditions and after treatment with a thermal or chemical stress. Levels of free ubiquitin fell slightly, ubiquitin conjugate levels rose shortly after stress began, and both then increased substantially as one of the cell's ubiquitin-encoding genes was activated by stress. The level of a protein synthesized as the carboxyl-terminal domain of one ubiquitin fusion protein was unchanged by a heat stress. The most dramatic effect was seen in the rapid disappearance of the ubiquitinated form of histone H2A, one of the major ubiquitin conjugates in cells in the interphase portion of their growth cycle. A significant rise in protein turnover was detected as a result of the stress, but occurred only when cells were removed from the stress condition. These results suggest that ubiquitin plays an important role both during and after stress, but fails to support hypotheses for ubiquitin and proteolysis in the activation of stress genes.
authors.affiliation: Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: U
authors.initials: U
authors.lastname: Bond
authors.forename: N
authors.initials: N
authors.lastname: Agell
authors.forename: A L
authors.initials: AL
authors.lastname: Haas
authors.forename: K
authors.initials: K
authors.lastname: Redman
authors.forename: M J
authors.initials: MJ
authors.lastname: Schlesinger
mesh_terms: D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D002478:Cells, Cultured; D002642:Chick Embryo; D005347:Fibroblasts / Q000032:analysis*; D006360:Heat-Shock Proteins / Q000032:analysis; D006358:Hot Temperature; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism*; D014452:Ubiquitins / Q000032:analysis*
chemical_list: D018053:Arsenites; D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D014452:Ubiquitins; C015001:arsenite; D001151:Arsenic