Inhibition by cAMP of the phosphoinositide response to alpha 1-adrenoceptor stimulation in rat kidney.
The effect of increasing intracellular levels of cAMP has been investigated on the PI response to stimulation of alpha 1-adrenoceptors in rat kidney. Stimulation of adenylate cyclase with forskolin or incubation with dibutyryl cAMP (dcAMP) inhibited noradrenaline (NA)-stimulated [3H]inositol phosphate (IP) accumulation. Forskolin did not alter the EC50 to NA. No effect on NA-stimulated [3H]IP accumulation was seen with dibutyryl cGMP (dcGMP). The results suggest that hormones which produce alterations in cAMP levels influence alpha 1-adrenoceptor-mediated responses.
Neylon
C B
CB
Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia.
Summers
R J
RJ
D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism / Q000502:physiology*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*
D010716:Phosphatidylinositols; D011942:Receptors, Adrenergic, alpha; D005576:Colforsin; D000242:Cyclic AMP; D009638:Norepinephrine