Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.
We have previously identified a cellular transcription factor, ATF, which is involved in the expression of multiple adenovirus E1a-inducible genes. Here we show that ATF also binds to regulatory elements in cellular cAMP-inducible promoters. In these cellular promoters, ATF binds to a consensus sequence that has been previously shown to be necessary and in some instances sufficient for cAMP-inducible transcription. Furthermore, we show that binding of ATF to these promoter elements is required for the constitutive in vitro transcriptional activity of the cAMP-inducible somatostatin gene. Taken together, our results suggest that a common cellular transcription factor, ATF, can be regulated in vivo by two apparently unrelated inducing agents: the adenovirus E1a protein and cAMP.
Lin
Y S
YS
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Green
M R
MR
D051696:Activating Transcription Factors; D000256:Adenoviridae / Q000235:genetics*; D017456:Adenovirus Early Proteins; D000957:Antigens, Viral, Tumor; D001483:Base Sequence; D001798:Blood Proteins / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D005786:Gene Expression Regulation; D005796:Genes; D005809:Genes, Regulator; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D014157:Transcription Factors / Q000378:metabolism*
D051696:Activating Transcription Factors; D017456:Adenovirus Early Proteins; D000957:Antigens, Viral, Tumor; D001798:Blood Proteins; D009856:Oncogene Proteins, Viral; D014157:Transcription Factors; D000242:Cyclic AMP