title: Expression of cholinesterase genes in human oocytes revealed by in-situ hybridization.
abstract: Transcriptional activity of the human cholinesterase genes was examined in developing oocytes from mature ovaries by in-situ hybridization combined with biochemical acetylcholine hydrolysis measurements. High levels of cholinesterase mRNA could be detected in oocytes from primordial, pre-antral and antral follicles but not in atretic follicles, with transient enhancement at the pre-antral stage. Biochemical analysis of enzymatic activity identified the ovarian enzyme as 'true' acetylcholinesterase by its sensitivity to selective inhibitors. Our findings suggest that cholinergic responses may function in human oocytes independently of the surrounding follicular cells, and the pronounced synthesis of cholinesterase transcripts in oocytes suggests that the cholinesterase genes in humans are particularly good candidates for the formation and re-insertion of inheritable processed cholinesterase genes.
authors.affiliation: Department of Biological Chemistry, Hebrew University, Jerusalem, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Soreq
authors.forename: G
authors.initials: G
authors.lastname: Malinger
authors.forename: H
authors.initials: H
authors.lastname: Zakut
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000235:genetics* / Q000378:metabolism; D002802:Cholinesterases / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases; D004262:DNA Restriction Enzymes