[Culture and control of cells producing bovine leukemia virus].
In the field surveys of the occurrence of enzootic bovine leucosis caused by the bovine leucosis virus (BLV), the identification of positive animals is based on the detection of specific antiviral antibodies by serological methods. The reliability of these tests (particularly their sensitivity and specificity) depends on the quality of the virus antigen. The preparation of the antigen is based on the cultivation of BLV virus in cultures of the FLS cell line. A modified procedure of preparing the BLV antigen in the FLS cell culture is described, along with the control of its production by the immunoperoxidase test.
Granátová
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Výzkumný ústav veterinárniho lékarství, Brno.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002478:Cells, Cultured; D007124:Immunoenzyme Techniques; D001909:Leukemia Virus, Bovine / Q000254:growth & development* / Q000276:immunology; D008722:Methods; D012190:Retroviridae / Q000254:growth & development*; D012756:Sheep
D000956:Antigens, Viral
Kultivace a kontrola bunĕk produkujících bovinní leukemický virus.