title: Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans.
abstract: A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacZ of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E. coli photolyases, notwithstanding the difference in chromophore structure.
authors.affiliation: Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yasui
authors.forename: M
authors.initials: M
authors.lastname: Takao
authors.forename: A
authors.initials: A
authors.lastname: Oikawa
authors.forename: A
authors.initials: A
authors.lastname: Kiener
authors.forename: C T
authors.initials: CT
authors.lastname: Walsh
authors.forename: A P
authors.initials: AP
authors.lastname: Eker
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics*; D005796:Genes; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004262:DNA Restriction Enzymes; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase