Differential action of nerve growth factor, cyclic AMP and neurotropin on PC12h cells.
Nerve growth factor (NGF) induced the activities of acetylcholinesterase (AChE) and Na+,K+-ATPase concomitant with neurite outgrowth in PC12h cells, while dibutyryl cyclic AMP (DBcAMP) caused the induction of AChE activity and neurite outgrowth but not Na+,K+-ATPase activity. A nonproteinaceous extract isolated from the inflamed skin of rabbits inoculated with vaccinia virus (Neurotropin) induced neurite outgrowth and cell surface change similar to NGF without affecting AChE activity. The results suggest that NGF, DBcAMP and Neurotropin act on PC12h cells through different mechanisms.
Morita
S
S
Department of Cell Biology and Microbiology, Nippon Zoki Pharmaceutical Co., Ltd., Hyogo, Japan.
Takeoka
Y
Y
Imai
H
H
Yamamoto
H
H
Suehiro
S
S
Ueda
S
S
Katoh
S
S
D000110:Acetylcholinesterase / Q000378:metabolism; D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000473:pathology; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism
D000700:Analgesics; D009414:Nerve Growth Factors; D011134:Polysaccharides; C010491:neurotropin; D003994:Bucladesine; D000110:Acetylcholinesterase; D000254:Sodium-Potassium-Exchanging ATPase