title: Different sensitivities of the responses of human neutrophils stimulated with immune complex and C5a anaphylatoxin to pertussis toxin.
abstract: When stimulated with immune complex or C5a anaphylatoxin, human neutrophils undergo an increase in the concentration of intracellular Ca2+ [( Ca2+]i) that precedes the onset of superoxide (O2-) production. The extracellular Ca2+ is required for the O2- production of neutrophils stimulated by C5a, but is only partially required for those by immune complex. The addition of pertussis toxin to neutrophils does not inhibit the rise in [Ca2+]i and O2- production induced by immune complex but does inhibit those induced by C5a. These results suggest that a different sequence of reaction is involved by different stimulants, the anaphylatoxin and immune complex.
authors.affiliation: Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shirato
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Nagasawa
authors.forename: J
authors.initials: J
authors.lastname: Koyama
mesh_terms: D000936:Antigen-Antibody Complex / Q000276:immunology*; D001572:Benzofurans; D002118:Calcium / Q000097:blood / Q000494:pharmacology; D003182:Complement C5 / Q000276:immunology*; D015936:Complement C5a; D005456:Fluorescent Dyes; D016257:Fura-2; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007700:Kinetics; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D037342:Pertussis Toxin; D013481:Superoxides / Q000097:blood; D013744:Tetanus Toxin / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D000936:Antigen-Antibody Complex; D001572:Benzofurans; D003182:Complement C5; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D013744:Tetanus Toxin; D010566:Virulence Factors, Bordetella; D013481:Superoxides; D015936:Complement C5a; D037342:Pertussis Toxin; D002118:Calcium; D016257:Fura-2