title: A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.
abstract: The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents.
authors.affiliation: Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
authors.forename: M C
authors.initials: MC
authors.lastname: Peitsch
authors.forename: T J
authors.initials: TJ
authors.lastname: Kovacsovics
authors.forename: H
authors.initials: H
authors.lastname: Isliker
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003166:Complement Activating Enzymes / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D003172:Complement C1 / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D015923:Complement C1r; D003173:Complement C1s / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D003171:Complement Pathway, Classical; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans
chemical_list: D003172:Complement C1; D004792:Enzyme Precursors; D003166:Complement Activating Enzymes; D015923:Complement C1r; D003173:Complement C1s