[Methodologic variants of DNA blood stain analysis: extraction in agarose and digoxigenin marking].
DNA was extracted from 1 microliter bloodstains on glass, cotton cloth and paper using proteinase K digestion in a solution containing low gelling temperature agarose. This method is advantageous for small amounts of source material because the purifying procedures of other methods that contain a risk of loss of DNA, are avoided. The recovered DNA was digested with Hae III and after Southern blotting, a Digoxigenin labeled probe (Amprobe, human Y-chromosome specific repeat) was successfully used for sex determination. With this non-radioactive method quantities of DNA smaller than 1.5 pg were detected.
Prinz
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M
Berghaus
G
G
Staak
M
M
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001801:Blood Stains; D015342:DNA Probes; D004076:Digoxigenin; D004077:Digoxin / Q000031:analogs & derivatives; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D012732:Sex Determination Analysis; D014998:Y Chromosome
D015342:DNA Probes; D005819:Genetic Markers; D004077:Digoxin; D004076:Digoxigenin
Methodische Varianten der DNA-Blutspurenanalyse: Extraktion in Agarose und Digoxigenin-Markierung.