title: Fungal mycelia as a novel source of eicosapentaenoic acid. Activation of enzyme(s) involved in eicosapentaenoic acid production at low temperature.
abstract: Several filamentous fungi belonging to the genus Mortierella were found to produce large amounts of 5,8,11,14,17-cis-eicosapentaenoic acid (EPA) in their mycelia only when grown at low temperature (12 degrees C), i.e., not at physiological growth temperature (20-28 degrees C). The results of experiments with cell-free extracts suggested that this unique phenomenon is due to activation of enzyme(s) involved in EPA formation at low temperature. Mortierella alpina 1S-4 produced 0.3 g/l of EPA (27 mg/g dry mycelia). This high productivity show the practical significance of these novel EPA producers.
authors.affiliation: Department of Agricultural Chemistry, Kyoto University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.forename: Y
authors.initials: Y
authors.lastname: Shinmen
authors.forename: H
authors.initials: H
authors.lastname: Kawashima
authors.forename: K
authors.initials: K
authors.lastname: Akimoto
authors.forename: H
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D003065:Coenzyme A / Q000494:pharmacology; D003080:Cold Temperature; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D004789:Enzyme Activation; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D009090:Mucorales / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides; D015636:Magnesium Chloride; D009249:NADP; D000255:Adenosine Triphosphate; D015118:Eicosapentaenoic Acid; D008274:Magnesium; D003065:Coenzyme A