Failure of simian virus 40 small t antigen to disorganize actin cables in nonpermissive cell lines.
Mouse C3H 10T1/2 cell lines expressing the simian virus 40 (SV40) small t antigen were obtained by cotransfection of pSV2neo and plasmids which encode small t. Cell lines derived from two plasmids which encode small t in the absence of stable deletion fragments of the large T antigen were morphologically normal and grew to slightly higher saturation densities in low serum than control cell lines. Unexpectedly, the clones had highly organized actin cables, as did parental 10T1/2 cells infected with wild-type SV40. These observations and comparisons of rat F111 cells infected with either polyomavirus or SV40 suggest that the SV40 small t antigen does not directly affect cytoskeletal organization.
Phillips
B
B
Cancer Center, Medical School, Northwestern University, Chicago, Illinois 60611.
Rundell
K
K
D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D000199:Actins / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D003599:Cytoskeleton / Q000648:ultrastructure*; D005347:Fibroblasts / Q000382:microbiology / Q000648:ultrastructure; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D010957:Plasmids; D011120:Polyomavirus / Q000502:physiology; D051381:Rats; D011994:Recombinant Proteins; D013539:Simian virus 40 / Q000235:genetics / Q000502:physiology*
D000199:Actins; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins