Atriopeptin 2 is hydrolysed by cardiac but not pulmonary isozyme of angiotensin-converting enzyme.
Hydrolysis of Bz-Gly-Ser-Phe-Arg, C-terminal fragment of atriopeptin 2, by human cardiac angiotensin-converting enzyme has been studied. The KM for the reaction was 10(-4) M. The effect of concentration of NaCl on activity of cardiac angiotensin-converting enzyme has been determined, which allowed to regard Bz-Gly-Ser-Phe-Arg as bradykinin-like substrates. It was demonstrated that cardiac, but not pulmonary isozyme of angiotensin-converting enzyme specifically hydrolyses atriopeptin 2.
Sakharov
I Y
IY
Institute of Experimental Cardiology, CRC AMS USSR, Moscow.
Dukhanina
E A
EA
Molokoedov
A S
AS
Danilov
S M
SM
Ovchinnikov
M V
MV
Bespalova
Z D
ZD
Titov
M I
MI
D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007527:Isoenzymes / Q000378:metabolism*; D008168:Lung / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D009928:Organ Specificity; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*
D007527:Isoenzymes; D009320:Atrial Natriuretic Factor; D007703:Peptidyl-Dipeptidase A