title: Re-evaluation of the intratesticular level of testosterone required for quantitative maintenance of spermatogenesis in the rat.
abstract: The amount of testosterone required for quantitative maintenance of spermatogenesis has been re-evaluated using techniques aimed at minimizing the synthesis of testosterone after removal of the testis. Adult male rats were treated with ethane dimethane-sulphonate (EDS) to destroy the Leydig cells, and were supplemented with 25, 5 or 1 mg testosterone esters by injection every 3 days for 21 days. Serum hormone levels, testicular morphology and spermatogenesis were assessed and the intratesticular levels of testosterone compared in testes either removed under ether anaesthesia and placed in liquid nitrogen (right testis) or removed after collection of blood and placed in ice (left testis). Data for testosterone-supplemented rats were compared with those for control rats and rats treated with EDS alone. All doses of testosterone suppressed LH and FSH levels in serum to within the hypophysectomized range, and Leydig cell regeneration in EDS-treated rats was prevented completely. Treatment of EDS-injected rats with 25 or 5 mg testosterone maintained testicular weight, the number of germ cells and the diameter of seminiferous tubules at stage VII within or above the control range, although there was a significant increase in the number of degenerating pachytene spermatocytes at stage VII with 5 but not 25 mg testosterone; none of these parameters was maintained at control levels by a dose of 1 mg testosterone. Levels of testosterone in testosterone-supplemented rats differed little between testes collected in ice and liquid nitrogen, but in controls and rats treated with EDS alone, testosterone levels were overestimated by 75 and 27% respectively when comparing testes collected in ice with those collected in liquid nitrogen.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh.
authors.forename: R M
authors.initials: RM
authors.lastname: Sharpe
authors.forename: K
authors.initials: K
authors.lastname: Donachie
authors.forename: I
authors.initials: I
authors.lastname: Cooper
mesh_terms: D000818:Animals; D005069:Evaluation Studies as Topic; D008297:Male; D008698:Mesylates / Q000494:pharmacology; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000166:cytology / Q000187:drug effects; D013739:Testosterone / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D008698:Mesylates; D013739:Testosterone; C002994:ethylene dimethanesulfonate