Mediastinal large-cell lymphoma with sclerosis. Genotypic analysis establishes its B nature.
The Southern blot hybridization technique has been applied to study the configuration of immunoglobulin and T-cell receptor genes in 6 cases of the so called mediastinal large cell lymphoma with sclerosis. This lymphoma has been recently recognized as a separate entity among non-Hodgkin lymphomas mainly affecting young adult patients. The B-cell origin of this neoplasm was suggested by means of immunohistochemical analysis. However, the immunophenotypical B-cell related markers used do not always exhibit lineage fidelity. The Southern blot analysis demonstrated the presence of unique heavy and k-light chain immunoglobulin gene rearrangements, establishing genotypically their B-cell origin.
Scarpa
A
A
Istituto di Anatomia, University of Verona, Policlinico Borgo Roma, Italy.
Bonetti
F
F
Menestrina
F
F
Menegazzi
M
M
Chilosi
M
M
Lestani
M
M
Bovolenta
C
C
Zamboni
G
G
Fiore-Donati
L
L
D001402:B-Lymphocytes / Q000276:immunology; D004273:DNA, Neoplasm / Q000032:analysis; D005803:Genes, Immunoglobulin; D005838:Genotype; D006801:Humans; D007118:Immunoassay; D007136:Immunoglobulins / Q000032:analysis / Q000235:genetics; D007150:Immunohistochemistry; D008223:Lymphoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008479:Mediastinal Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D009693:Nucleic Acid Hybridization; D011956:Receptors, Cell Surface / Q000235:genetics; D012598:Sclerosis; D013601:T-Lymphocytes
D004273:DNA, Neoplasm; D007136:Immunoglobulins; D011956:Receptors, Cell Surface