Bacillus subtilis mutant deficient in the ability to produce the dipeptide antibiotic bacilysin: isolation and mapping of the mutation.
A Bacillus subtilis mutant which carries a lesion in a gene specific to the synthesis of the dipeptide antibiotic bacilysin was isolated. A derivative strain in which transposon Tn917 had inserted near the bacilysin lesion was isolated and used as the donor in PBS-1 transduction mapping experiments. The bac-1 locus was mapped between the ctrA and sacA loci, near 90% on the standard B. subtilis 168 chromosome map.
Hilton
M D
MD
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139.
Alaeddinoglu
N G
NG
Demain
A L
AL
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004151:Dipeptides / Q000096:biosynthesis / Q000235:genetics; D005798:Genes, Bacterial; D009154:Mutation; D014161:Transduction, Genetic
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004151:Dipeptides; C006674:bacilysin