ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.
Simian virus 40 large T antigen initiates DNA replication by binding to the origin of replication. We examined the binding of T antigen to origin regions I, II, and III under conditions designed for efficient in vitro replication functions. We found that 4 mM ATP enhanced the binding of T antigen to regions I and II of the origin DNA by 4- to 20-fold. DNase-footprinting and fragment assays showed that ATP extended the DNase protection domain of T antigen bound to region II by 5 to 10 base pairs at both ends of the core origin of replication. This alteration suggests a change in the conformation of T antigen, bound DNA, or both.
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Department of Microbiology, State University of New York, Stony Brook 11794-8261.
Tegtmeyer
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D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009710:Nucleotide Mapping; D009711:Nucleotides / Q000378:metabolism; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D009711:Nucleotides; D000255:Adenosine Triphosphate; D003850:Deoxyribonuclease I