title: [Isolation and properties of the SuaI restriction endonuclease from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius].
abstract: A new restriction endonuclease SuaI was isolated from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. The enzyme is an isoschizomer of BspR1; it recognizes tetranucleotide GGCC and cleaves DNA in the center of this sequence. SuaI requires Mg2+, the optimal concentration being 6 mM. KCl at concentrations above 25 mM significantly inhibits the enzyme activity. The pH optimum lies within the range of 6--7 at 70 degrees C, the temperature optimum is at 70--75 degrees C. The enzyme is highly stable at temperatures up to 80 degrees C. DNA of S. acidocaldarius is not cleaved by the enzyme.
authors.forename: D A
authors.initials: DA
authors.lastname: Prangishvili
authors.forename: R P
authors.initials: RP
authors.lastname: Vashakidze
authors.forename: M G
authors.initials: MG
authors.lastname: Chelidze
authors.forename: D Z
authors.initials: DZ
authors.lastname: Chinchaladze
authors.forename: N V
authors.initials: NV
authors.lastname: Tsalkalamanidze
mesh_terms: D001105:Archaea / Q000201:enzymology*; D001419:Bacteria / Q000201:enzymology*; D001483:Base Sequence; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004262:DNA Restriction Enzymes / Q000032:analysis / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D004795:Enzyme Stability; D006358:Hot Temperature; D010957:Plasmids; D013379:Substrate Specificity
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases
vernacular_title: Vydelenie i svoĭstva éndonukleazy restriktsii SuaI iz termoatsidofil'noĭ arkhebakterii Sulfolobus acidocaldarius.