The use of glucose dehydrogenase to monitor the integrity of microsomes.
Various concentrations of Tergitol NP-10 stimulate mannose-6-phosphatase and glucose dehydrogenase to the same extent in untreated rat liver microsomes. Thus, the latency of glucose dehydrogenase may be used as an alternative to mannose phosphatase as a measure of the integrity of the microsomal membrane. The advantage of using glucose dehydrogenase rather than mannose phosphatase to monitor microsomal integrity is that NADH is more easily measured than Pi.
Bublitz
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Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262.
Steavenson
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D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D003902:Detergents; D004492:Edetic Acid / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology*; D009243:NAD / Q000032:analysis / Q000378:metabolism; D017137:Nonoxynol; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013053:Spectrophotometry
D003902:Detergents; D009243:NAD; D017137:Nonoxynol; D011092:Polyethylene Glycols; D004492:Edetic Acid; D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; C023553:mannose-6-phosphatase; D010744:Phosphoric Monoester Hydrolases