[Na,K-ATPase activity of erythrocyte ghosts of mammals in the presence of alamethicin and p-nitrophenylphosphate].
After preincubation of erythrocyte ghosts isolated from Sprague-Dawley rats activity of Na, K-ATPase was more successfully manifested, being elevated about 2.5-fold, while the ratio Mg-ATPase/Na, K-ATPase was decreased 3-fold. Optimal ratio of alamethicin/protein was equal to 0.3. The similar results were obtained if p-nitrophenyl phosphate, easily penetrating through the erythrocyte membrane, was used as an exogenous substrate.
Sokol'nikov
A A
AA
Kodentsova
V M
VM
Gulidova
G P
GP
Mironova
G D
GD
D000408:Alamethicin / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004910:Erythrocyte Membrane / Q000201:enzymology*; D066298:In Vitro Techniques; D008297:Male; D009596:Nitrophenols / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*; D013379:Substrate Specificity
D000900:Anti-Bacterial Agents; D003470:Culture Media; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D000408:Alamethicin; C008644:nitrophenylphosphate; D000254:Sodium-Potassium-Exchanging ATPase
Aktivnost' Na,K-ATFazy v teniakh éritrotsitov mlekopitaiushchikh v prisutstvii alametitsina i p-nitrofenilfosfata.