Adrenal enkephalin biosynthesis regulated by glucocorticoid.
Denervation of the rat adrenal results in a 10 to 20-fold increase in enkephalin-containing EC peptides 4 days after surgery. This denervation-induced rise in medullary EC peptides is blocked by hypophysectomy (hypox) and partially reinstated by dexamethasone or ACTH treatment. Culture of adrenal medullary explants (glands) for 4 days in serum-free medium without dexamethasone resulted in a 6-fold increase in EC peptides in glands from hypox rats while the addition of dexamethasone (10-5M) produced a 29 fold increase. These results indicate that a loss of depolarizing stimuli produced by denervation in vivo or explanation in vitro initiates a pretranslational event which requires glucocorticoid for the full expression of EC peptide biosynthesis.
Inturrisi
C
C
Department of Pharmacology, Cornell University Medical College, New York, New York.
Franklin
S
S
Shapiro
J
J
Calvano
S
S
Yoburn
B
B
D000313:Adrenal Medulla / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D003714:Denervation; D003907:Dexamethasone / Q000494:pharmacology*; D004745:Enkephalins / Q000096:biosynthesis*; D007016:Hypophysectomy; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D004745:Enkephalins; D003907:Dexamethasone; D000324:Adrenocorticotropic Hormone