Comparison of serological tests for antibodies against Newcastle disease virus and infectious bronchitis virus using ImmunoComb solid-phase immunoassay, a commercial enzyme-linked immunosorbent assay, and the hemagglutination-inhibition assay.
COMBSCORES determined using the ImmunoComb solid-phase immunoassay were compared with hemagglutination-inhibition (HI) titers specific for Newcastle disease virus (NDV) and infectious bronchitis virus (IBV) and with mean enzyme-linked immunosorbent assay (ELISA) titers determined using Agritech Systems, Inc., ELISA. COMBSCORES for NDV and IBV increased proportionately in a stepwise manner as HI titers increased. The ImmunoComb solid-phase immunoassay was ablt to produce endpoint titers on sera with NDV-HI titers of 0 through 320 and IBV-HI titers of 0 through 1024 without reaching the maximum S-value. The ImmunoComb showed good correlation with the HI assay and the Agritech ELISA and should prove to be a useful tool for serological profiling, either alone or in conjunction with the HI test or commercial ELISA.
Thayer
S G
SG
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30605.
Nersessian
B N
BN
Rivetz
B
B
Fletcher
O J
OJ
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D003332:Coronaviridae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007118:Immunoassay; D007159:Immunologic Tests; D001351:Infectious bronchitis virus / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology*; D011200:Poultry; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012044:Regression Analysis; D012984:Software
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic