In vitro splicing of mRNA precursors: 5' cleavage site can be predicted from the interaction between the 5' splice region and the 5' terminus of U1 snRNA.
Combinations of different mutations within the 5' splice region of the rabbit beta-globin large intron were analyzed for their effect on in vitro splicing. Based upon the complementarity of the 5' splice region to the 5' terminal region of the U1 snRNA, the exact location of the 5' cleavage site of different mutants could be predicted and was experimentally confirmed. These findings add further strong support to the hypothesis (1) that the exact location of the 5' cleavage site in pre-mRNA splicing of higher eukaryotes is determined by the overall 5' splice region via the complementarity to the 5' end of the U1 snRNA, and not by the strongly conserved GU dinucleotide.
Weber
S
S
Institut für Molekularbiologie I, Universität Zürich, Switzerland.
Aebi
M
M
D004252:DNA Mutational Analysis; D004720:Endonucleases; D066298:In Vitro Techniques; D009694:Nucleic Acid Precursors / Q000235:genetics*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases
D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases