Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
Oligodeoxynucleotides lead to translation arrest of complementary mRNAs in the wheat germ translation system by a degradation of the mRNA. In an attempt to develop an effective reverse genetic approach in vivo, we demonstrate that injection of short (15- to 30-nucleotide) oligonucleotides into Xenopus oocytes leads to complete degradation of both injected and endogenous mRNAs by means of an RNase H-like activity.
Dash
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Howard Hughes Medical Institute, Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, NY.
Lotan
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Knapp
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Kandel
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ER
Goelet
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D000818:Animals; D001483:Base Sequence; D002147:Calmodulin / Q000235:genetics; D002474:Cell-Free System; D004722:Endoribonucleases / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D009865:Oocytes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D016914:Ribonuclease H; D014908:Triticum; D014982:Xenopus laevis
D002147:Calmodulin; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D005914:Globins; D004722:Endoribonucleases; D016914:Ribonuclease H