Distribution of calpains and calpastatin in human blood cells.
The occurrence and molecular sizes of calpains and calpastatin in the lysates of human erythrocytes, platelets, lymphocytes/monocytes, and polymorphonuclear cells were studied by immunoelectrophoretic blot analysis. The basic uniformity among these cells of the 85-kDa and 83-kDa heavy subunits of low- and high-Ca2+-requiring calpains I and II, respectively, and of the 29-kDa light subunit was confirmed. Molecular diversity of calpastatin species, ranging from 70 kDa to 107 kDa, among different blood cells was also shown. The obtained data are consistent with those known for other animal tissues, thus settling hitherto uncertain or rather controversial issues on the distribution of calpains and calpastatin in human blood cells.
Takano
E
E
Department of Clinical Science and Laboratory Medicine, Kyoto University Faculty of Medicine, Japan.
Park
Y H
YH
Kitahara
A
A
Yamagata
Y
Y
Kannagi
R
R
Murachi
T
T
D001792:Blood Platelets / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000097:blood*; D002154:Calpain / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D011480:Protease Inhibitors / Q000097:blood*
D002135:Calcium-Binding Proteins; D011480:Protease Inhibitors; C033668:calpastatin; D002154:Calpain