Binding of C-terminal segments of neuropeptide Y to chicken brain.
The C-terminal pentapeptide amide segment of neuropeptide Y (NPY) binds specifically to chicken brain membrane preparations. The contribution of each residue of the C-terminus to this binding has been investigated through the synthesis and evaluation of a series of pentapeptide analogs. The binding of these molecules is strongly dependent on the presence of certain functional groups, in particular the guanidinium group of Arg-35 and the C-terminal aromatic amide function. The significance of these results for the binding to chicken brain tissue of NPY, pancreatic polypeptide and other potentially related neuropeptides is discussed.
Perlman
M O
MO
Department of Chemistry, University of Houston, Texas.
Perlman
J M
JM
Adamo
M L
ML
Hazelwood
R L
RL
Dyckes
D F
DF
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002645:Chickens; D007202:Indicators and Reagents; D007700:Kinetics; D009478:Neuropeptide Y / Q000378:metabolism*; D009842:Oligopeptides / Q000138:chemical synthesis*; D010446:Peptide Fragments / Q000378:metabolism; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013329:Structure-Activity Relationship
D007202:Indicators and Reagents; D009478:Neuropeptide Y; D009842:Oligopeptides; D010446:Peptide Fragments; D017476:Receptors, Neuropeptide Y; D017981:Receptors, Neurotransmitter