Wound fiberglass depth filters as a less expensive approach for the concentration of viruses from water.
Wound fiberglass depth cartridge filters (25.4 cm) with a nominal porosity of 1 micron were used to concentrate viruses from large volumes of surface water. They were found to be an excellent, less expensive alternative to the 0.2-micron pleated cartridge filters normally used for the concentration of enteric viruses from water. More than 99% of experimentally seeded poliovirus was adsorbed to these filters when the pH of the water was adjusted to pH 3.5 and aluminium chloride was added to a final concentration of 0.001 M, as recommended for electronegative filters. In comparative recovery of indigenous viruses from river water, similar results were obtained with two 1-micron or a 3-microns + 0.2-micron filter combination. The cost of the two 1-micron filters is about Can. $26, while it is about Can. $58 for the other combination.
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Centre de recherche en virologie, Institut Armand-Frappier, Ville de Laval, Qué., Canada.
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D000818:Animals; D002460:Cell Line; D005374:Filtration; D005618:Fresh Water; D005898:Glass; D006863:Hydrogen-Ion Concentration; D017955:Poliovirus / Q000254:growth & development / Q000302:isolation & purification*; D010948:Viral Plaque Assay; D014780:Viruses / Q000254:growth & development / Q000302:isolation & purification*; D014871:Water Microbiology
C009753:fiberglass