title: An internal 5'-noncoding region required for translation of poliovirus RNA in vitro.
abstract: A truncated poliovirus RNA that contains the entire 5'-noncoding region as well as some capsid protein-coding sequences was produced from cloned cDNA inserted into an SP6 transcription vector and subsequently was translated in a mixed rabbit reticulocyte-HeLa cell lysate. Deletions or modifications of regions of the 5'-noncoding sequences had significant effects upon the efficiency of translation. The presence of a 60-nucleotide sequence located at positions 567 to 627 appeared to be essential for active ribosome binding and translation of this uncapped RNA.
authors.affiliation: Department of Cellular Biology, University of Utah School of Medicine, Salt Lake City 84132.
authors.forename: K
authors.initials: K
authors.lastname: Bienkowska-Szewczyk
authors.forename: E
authors.initials: E
authors.lastname: Ehrenfeld
mesh_terms: D002474:Cell-Free System; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral