Isolation of three kinds of human endogenous retrovirus-like sequences using tRNA(Pro) as a probe.
Three kinds of human endogenous retrovirus-like sequences (HuERS-P1, 2 and 3) were isolated from a HeLa cell genomic library using the 3'-half fragment of proline tRNA as a hybridization probe. These elements contained putative primer binding sites complementary to the 3'-terminus of proline tRNA and long terminal repeats (LTRs) characteristic of retrovirus provirus. The LTR sequence of HuERS-P1 consisted of about 690 nucleotides and contained a CAT box, a TATA box and a polyadenylation signal. A complete unit of an Alu family sequence was inserted into the 5'-LTR of one of the clones. HuERS-P2 also contained a TATA box and a polyadenylation signal in its LTR (about 840 nucleotides long), but the LTR sequence of this element was quite different from that of HuERS-P1. Although clone HuERS-P3 contained only the 5'-LTR region, this LTR sequence contained a CAT box, a TATA box and a poly-adenylation signal and was quite similar to the LTR sequence of the recently isolated human retrovirus-related sequence HuRRS-P (Kröger, B. and Horak, I. (1987) J. Virol., 61, 2071-2075). Human and simian DNAs contain 10 to 40 copies of these elements, but mouse DNA does not contain these elements.
Harada
F
F
Biophysics Division, Kanazawa University, Japan.
Tsukada
N
N
Kato
N
N
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D011401:Promoter Regions, Genetic; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012361:RNA, Transfer, Pro / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral; D012345:RNA, Transfer, Amino Acid-Specific; D012361:RNA, Transfer, Pro; D004262:DNA Restriction Enzymes