Characterization of cyclic nucleotide phosphodiesterases in Xenopus laevis ovary.
A cGMP-stimulated cyclic nucleotide phosphodiesterase present in cytosol of Xenopus laevis ovary has been purified and characterized. A cAMP-specific phosphodiesterase which is not activated by either cGMP or calmodulin, has also been characterized. Brief exposure of intact oocytes to 10 micro M progesterone results in an increase in activity of the cAMP-specific enzyme. The cGMP-stimulated and the calmodulin-activated phosphodiesterases are not altered. Changes in cyclic nucleotide levels during progesterone-induced maturation of oocytes may be modulated by these isoenzymes.
Allende
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C
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago.
Plaza
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M
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000818:Animals; D006152:Cyclic GMP / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D010053:Ovary / Q000201:enzymology*; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D013379:Substrate Specificity; D014982:Xenopus laevis / Q000378:metabolism*
D009712:Nucleotides, Cyclic; D011374:Progesterone; D010727:Phosphoric Diester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D006152:Cyclic GMP