Inhibitory effect of parvovirus H-1 on cultured human tumour cells or transformed cells.
The present study establishes the in vitro system for studying the oncosuppression activity of parvovirus and discovers that parvovirus H-1 can be grown cytolytically in various human cancer cell lines, which include 4 hepatoma cell lines (QGY-7703, SMMC-7721, Bel-7402, PLC/PRF/5), 3 gastric cancer cell lines (SGC-7901, MGC-80-3, MKN-28) and 1 naspharynx cancer cell line (CNE). The growth of two primary gastric cancer cell cultures from surgical cancer tissue were also inhibited by the infection of H-1. The sensitivity of cancer cells to H-1 may relate to their differentiation states. On the contrary, H-1 can neither be grown cytolytically in normal liver or stomach cells, nor inhibit their growth. Transformation of human skin fibroblasts with Simian Virus 40 activated their sensitivity to H-1. Our results thus indicate that the antineoplastic activity of H-1 in vivo involves at least its direct inhibiting or killing malignant cells.
Su
Z Z
ZZ
Department of Biology, Fudan University, Shanghai.
Luo
Z Y
ZY
Guo
L P
LP
Li
J Z
JZ
Liu
Y L
YL
D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002452:Cell Count; D002454:Cell Differentiation; D002460:Cell Line; D002472:Cell Transformation, Viral; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D010321:Parvoviridae / Q000502:physiology*; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured