A method for estimating the concentration of spermatozoa in the rat cauda epididymidis.
A method for estimating the concentration of spermatozoa in the rat cauda epididymidis is described. Treatment of a sperm suspension with 0.05% collagenase for 20-60 min or 0.025% trypsin for 1-2 min at 34-37 degrees C was found to result in consistently homogeneous sperm. Sperm concentration ranged from 152.5 to 230.0 X 10(7) spermatozoa/ml, with a mean of 187.7 (+/- 5.6 SEM) X 10(7) spermatozoa/ml.
Kempinas
W G
WG
Department of Stomatology, Faculty of Dentistry of Ribeirão Preto, SP, Brazil.
Lamano-Carvalho
T L
TL
D000818:Animals; D004822:Epididymis; D008297:Male; D003012:Microbial Collagenase; D051381:Rats; D011919:Rats, Inbred Strains / Q000502:physiology*; D013076:Sperm Count / Q000662:veterinary*; D014357:Trypsin
D014357:Trypsin; D003012:Microbial Collagenase