title: Partial characterization of a bovine group A rotavirus with a short genome electropherotype.
abstract: A group A rotavirus (ID isolate) recovered from a diarrheic beef calf possessed a short genome electropherotype. This short genome electropherotype was a stable characteristic of the ID isolate as it remained unchanged through 3 passages in gnotobiotic calves or through 19 passages in MA104 cell cultures. Subgroup analysis with monoclonal antibodies in an enzyme-linked immunosorbent assay established that the isolate was a subgroup 1 rotavirus. Neutralization tests demonstrated that this isolate was a distinct serotype from the human group A rotavirus S2 isolate (short genome electropherotype) and the turkey group A rotavirus 174 isolate (semi-short genome electropherotype). The ID isolate was pathogenic for 5- to 21-day-old gnotobiotic calves, inducing diarrhea within 48 h postinoculation.
authors.affiliation: Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
authors.forename: K W
authors.initials: KW
authors.lastname: Theil
authors.forename: C M
authors.initials: CM
authors.lastname: McCloskey
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D005814:Genes, Viral; D009500:Neutralization Tests; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral