Immunological mechanisms giving rise to latency of herpes simplex virus in the spinal ganglia of the mouse.
In the model of genital herpes simplex virus (HSV)-infection of mice, early latency could be induced by passive immunization with HSV-specific antibodies and, to a lesser degree, by adoptive transfer of immune lymphocytes prepared from spleen and draining lymph nodes of genitally infected syngeneic mice. Conversely, spontaneously occurring latency was inhibited by treatment of the animals with cyclophosphamide (Cph) and, to a lesser degree, with cyclosporin A (CyA). Whereas the effect of CyA could be compensated by passively administered HSV-specific antibodies, that of Cph could not. Apparently specific antibodies cooperate with a non-specific proliferating cell type, probably macrophages and/or NK-cells, as could be demonstrated by significantly reduced antibody effect in silica-treated mice. Moreover, F(ab)2 fragments, in contrast to complete antibody molecules, were inactive. HSV-specific antibodies and also immune lymphocytes had little effect on virus production in the mucous membranes, immune lymphocytes being at least as active as antibodies. It is therefore not probable that latency is induced by attenuation of the peripheral disease. It can rather be concluded that the neuron itself is the target for the action of specific antibodies, cooperating in turn with macrophages and/or NK cells.
Schneweis
K E
KE
Institut für Med. Mikrobiologie und Immunologie der Universität, Bonn Federal Republic of Germany.
Brado
M
M
Ebers
B
B
Friedrich
A
A
Olbrich
M
M
Schüler
W
W
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D005727:Ganglia, Spinal / Q000276:immunology / Q000382:microbiology*; D006561:Herpes Simplex / Q000209:etiology / Q000276:immunology* / Q000382:microbiology; D007116:Immunization, Passive; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D051379:Mice; D012822:Silicon Dioxide / Q000494:pharmacology; D018139:Simplexvirus / Q000276:immunology / Q000302:isolation & purification; D013997:Time Factors
D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D007166:Immunosuppressive Agents; D012822:Silicon Dioxide