Identification of rat brain opioid (enkephalin) receptor by photoaffinity labeling.
A photoreactive, radioactive enkephalin derivative was prepared and purified by high performance liquid chromatography. Rat brain and spinal cord plasma membranes were incubated with this radioiodinated photoprobe and were subsequently photolysed. Autoradiography of the sodium dodecyl sulfate gel electrophoresis of the solubilized and reduced membranes showed that a protein having an apparent molecular weight of 46,000 daltons was specifically labeled, suggesting that this protein may be the opioid (enkephalin) receptor.
Yeung
C W
CW
Playfair Neuroscience Unit, University of Toronto, Ontario, Canada.
D000345:Affinity Labels / Q000138:chemical synthesis*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D007457:Iodine Radioisotopes; D051381:Rats; D011957:Receptors, Opioid / Q000302:isolation & purification / Q000378:metabolism*
D000345:Affinity Labels; D007457:Iodine Radioisotopes; D011957:Receptors, Opioid; D004744:Enkephalin, Methionine; C046968:enkephalin-Met, Ala(2)-4-azido-Phe(4)-