Identification of hog cholera viral isolates by use of monoclonal antibodies to pestiviruses.
A collection of 90 field isolates of hog cholera virus (HCV) was used to test the specificity of four hybridoma cell lines secreting monoclonal antibodies against pestiviruses. Reaction of virus isolates and monoclonal antibodies was controlled by an indirect immunofluorescence assay (IFA). Two monoclonal antibodies which had been generated against HC virus strain "Alfort 187" were reactive only with HCV field isolates and an HCV reference strain but not with bovine viral diarrhoea virus (BVDV) reference strains. Two other monoclonal antibodies (generated against BVDV, strain NADL) reacted only with BVDV reference strains but not with HCV field isolates, although with 3 of these strains focal reactions involving only a few cells were detected. The ability to discriminate between both viruses is a diagnostic need which may be fulfilled by these monoclonal antibodies.
Hess
R G
RG
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Coulibaly
C O
CO
Greiser-Wilke
I
I
Moennig
V
V
Liess
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000276:immunology / Q000302:isolation & purification*; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D010576:Pestivirus / Q000276:immunology*; D013552:Swine
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral