Isolation and characterization of restriction endonuclease BstYI from Bacillus stearothermophilus Y406.
BstYI, an isoschizomer of XhoII and MflI, has been purified from Bacillus stearothermophilus Y406. This enzyme recognized 5'...Pu/GATCPy...3' in DNA and cleaved between Pu and G in this sequence. BstYI can be easily isolated and purified by heparin-agarose column chromatography in a high yield (8000 units BstYI can be obtained per g wet wt of cells).
Chen
Z F
ZF
Institute of Genetics, Fudan University, Shanghai, P.R. China.
Kong
H M
HM
D010582:Bacteriophage lambda; D002498:Centrifugation; D011232:Chemical Precipitation; D002845:Chromatography; D002850:Chromatography, Gel; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C056536:endodeoxyribonuclease BstYI; D015252:Deoxyribonucleases, Type II Site-Specific