Evaluation of biotin-streptavidin enzyme-linked immunosorbent assay for detection of genital herpes simplex virus infection.
A biotin-streptavidin enzyme-linked immunosorbent assay (B-SA ELISA) was evaluated for detection of herpes simplex virus (HSV) in clinical specimens which were cervico-vaginal swabs from 205 asymptomatic women and swabs from the genital lesions of 163 suspected patients. All specimens were also subjected to a conventional virus isolation in cell culture. A blocking B-SA ELISA had 100% specificity and 98% sensitivity compared with viral isolation from patients, but had only 40% sensitivity using specimens from asymptomatics. The conventional B-SA ELISA might also be used; it gave results corresponding to B-SA ELISA blocking test except for a single specimen which was considered a false positive.
Yoosook
C
C
Department of Microbiology, Faculty of Science, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Rimdusit
P
P
Chantratita
W
W
Leechanachai
P
P
Bhattarakosol
P
P
D000956:Antigens, Viral / Q000032:analysis*; D001426:Bacterial Proteins; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005836:Genitalia, Female / Q000382:microbiology; D005837:Genitalia, Male / Q000382:microbiology; D006558:Herpes Genitalis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D018139:Simplexvirus / Q000276:immunology* / Q000302:isolation & purification; D019809:Streptavidin; D014626:Vaginal Smears
D000956:Antigens, Viral; D001426:Bacterial Proteins; D001710:Biotin; D019809:Streptavidin