Inhibition of the streptavidin-biotin interaction by milk.
Nonfat dried milk is routinely used as a blocking agent and diluent in immunoassays. However, the data presented in this paper demonstrate that milk contains an inhibitor of the biotin-streptavidin interaction. It is recommended that milk be dialyzed or used at lower concentrations when it is employed as a diluent of streptavidin.
Hoffman
W L
WL
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
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D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis*; D001710:Biotin / Q000032:analysis*; D015153:Blotting, Western; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003956:Dialysis; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D008892:Milk / Q000032:analysis*; D019809:Streptavidin
D001426:Bacterial Proteins; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D001710:Biotin; D019809:Streptavidin; D006735:Horseradish Peroxidase