Immune electron microscopy in the detection of viruses other than enteroviruses on cell culture in untreated sewage.
Following the demonstration of enteroviruses in samples of untreated sewage by inoculation of cell cultures with an eluate fraction, immune electron microscopy (IEM) was employed in two stages to detect adenoviruses and the hepatitis A virus (HAV). 6/12 samples contained adenoviruses and 5/15 other samples contained viruses having characteristics of HAV. Cell culture can thus be usefully allied with IEM in the more precise determination of the potential health hazards of waste water.
Pietri
C
C
Institut National de la Santé et de la Recherche Médicale, Unité 303, Nice, France.
Hugues
B
B
Puel
D
D
D000256:Adenoviridae / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D000914:Antibodies, Viral; D004770:Enterovirus / Q000254:growth & development / Q000302:isolation & purification*; D006507:Hepatovirus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D008854:Microscopy, Electron; D012722:Sewage; D014871:Water Microbiology
D000914:Antibodies, Viral; D012722:Sewage