In vivo effects of 1,25-dihydroxyvitamin D3 and minerals on synthesis of leukotriene B4 and 5-hydroxyeicosatetraenoic acid by rat peritoneal neutrophils.
We investigated the in vivo effects of 1,25-dihydroxyvitamin D3, and serum calcium and inorganic phosphorus concentrations on productions of leukotriene B4 and 5-hydroxyeicosatetraenoic acid by glycogen stimulated peritoneal neutrophils of the rat. Neutrophils were incubated with ionophore A23187 for 5 min and the incubation medium was subjected to a reverse-phase high performance liquid chromatography for quantitation. Productions of these lipoxygenase metabolites by neutrophils from vitamin D depleted rats were decreased compared to the vitamin D sufficient levels. This difference was not observed when arachidonic acid was added to the incubation medium. When serum calcium concentrations of rats were altered by dietary calcium levels but 1,25-dihydroxyvitamin D3 was administered to those rats, the difference in serum calcium concentration did not show any effect on the production of leukotriene B4. In contrast, neutrophils from 1,25-dihydroxyvitamin D3 sufficient but phosphorus deficient rats still demonstrated decreased leukotriene B4 production. This reduction was corrected by exogenous arachidonic acid. The data suggest that 1,25-dihydroxyvitamin D3 or cellular calcium translocation mediated by the hormone, and serum inorganic phosphorus independent of 1,25-dihydroxyvitamin D3 affect arachidonic acid release, thus productions of leukotriene B4 and 5-hydroxyeicosatetraenoic acid.
Tanaka
Y
Y
Veterans Administration Medical Center, Albany, New York.
Klauck
T M
TM
Jubiz
W
W
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010758:Phosphorus / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D010758:Phosphorus; D000001:Calcimycin; D002117:Calcitriol; D002118:Calcium