Influence of thiocompounds on the metabolism of N-nitrosodiethylamine.
Diethyldithiocarbaminate (DDTC), mercaptoethanesulfonate (MESNA) and ethylxanthate (PEX) were tested for their influence on N-nitrosodiethylamine (NDEA) metabolism. The exhalation rate of 14CO2 released from [14C]NDEA was decreased by PEX, but not by MESNA or DDTC. The sulfur compounds led to an increased excretion of unchanged NDEA in urine in the order PEX greater than DDTC much greater than MESNA. The activity of NDEA-deethylase in liver microsomal system was decreased only after DDTC or PEX treatment. Glutathione content and glutathione-S-transferase activity were not affected significantly by any of the tested compounds. NDEA-induced single-strand breaks in liver cell DNA were inhibited after PEX treatment.
Frank
N
N
Institute of Toxicology and Chemotherapy, German Cancer Research Center, Heidelberg.
Bertram
B
B
Frei
E
E
Hadjiolov
D
D
Wiessler
M
M
D000818:Animals; D004249:DNA Damage; D004052:Diethylnitrosamine / Q000378:metabolism*; D004050:Ditiocarb / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D015080:Mesna / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D013871:Thiones / Q000494:pharmacology
D013438:Sulfhydryl Compounds; D013871:Thiones; D004052:Diethylnitrosamine; D004050:Ditiocarb; C004918:ethylxanthate; D005982:Glutathione Transferase; D005978:Glutathione; D015080:Mesna