title: Influence of sugar source (lactose, glucose, galactose) on 2,3-butanediol production by Klebsiella oxytoca NRRL-B199.
abstract: The ability of Klebsiella oxytoca NRRL-B199 to use either lactose or the mixture of glucose and galactose as substrate for the production of 2,3-butanediol was studied in batch fermentations with different conditions of aeration and pH. 2,3-butanediol was undetected, or present in minute concentration in the fermentation broths with lactose, while it was the main product from glucose + galactose with final concentrations of up to 18.8g/l in media at pH 6.0. Under conditions optimal for 2,3-butanediol synthesis, when aeration limited growth, the rate of biomass growth was more tightly related to the aeration rate in lactose medium than in glucose + galactose medium. These relations suggest that the growth rate is very low on lactose but still considerable on glucose + galactose when aeration rate tends toward zero. Corresponding, the metabolism is more oxidative in the former medium, yielding mainly acetate as product.
authors.affiliation: Unité de Chimie des Interfaces, Universite Catholique de Louvain, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Champluvier
authors.forename: J
authors.initials: J
authors.lastname: Decallonne
authors.forename: P G
authors.initials: PG
authors.lastname: Rouxhet
mesh_terms: D002072:Butylene Glycols / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007709:Klebsiella / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D005947:Glucose; D007785:Lactose; D010100:Oxygen; D005690:Galactose