The promoting effects of food dyes, erythrosine (Red 3) and rose bengal B (Red 105), on thyroid tumors in partially thyroidectomized N-bis(2-hydroxypropyl)-nitrosamine-treated rats.
The effects of erythrosine (Red 3), rose bengal B (Red 105) and thyroidectomy on the development of thyroid tumor were examined in male Wistar rats treated with N-bis(2-hydroxypropyl)nitrosamine (DHPN). Red 3 and Red 105 were used at 4% in the basal diet and were administered for 19 weeks from week 2 to 20. Thyroidectomy was performed by resection of the left lobe at week 4. Single injection of DHPN was performed intraperitoneally at 280 mg per 100 g body weight at the beginning of the experiment. Red 3 and Red 105 significantly promoted the development of thyroid tumors in thyroidectomized rats given DHPN, but had no significant effect in non-thyroidectomized rats. The incidence of thyroid tumors was 91% in rats with partial thyroidectomy, Red 3 and DHPN, 100% in rats with partial thyroidectomy, Red 105 and DHPN, and 64% in rats with partial thyroidectomy and DHPN. Serum TSH was 5.5 +/- 3.1 ng/ml in rats with partial thyroidectomy, Red 3 and DHPN, 2.1 +/- 2.2 ng/ml in rats with partial thyroidectomy, Red 105 and DHPN, and 1.5 +/- 0.5 ng/ml in rats with partial thyroidectomy and DHPN.
Hiasa
Y
Y
First Department of Pathology, Nara Medical University.
Ohshima
M
M
Kitahori
Y
Y
Konishi
N
N
Shimoyama
T
T
Sakaguchi
Y
Y
Hashimoto
H
H
Minami
S
S
Kato
Y
Y
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004396:Coloring Agents; D004923:Erythrosine / Q000633:toxicity*; D005452:Fluoresceins / Q000633:toxicity*; D005505:Food Coloring Agents / Q000633:toxicity*; D008099:Liver / Q000187:drug effects; D008297:Male; D009374:Neoplasms, Experimental / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D009602:Nitrosamines; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012395:Rose Bengal / Q000031:analogs & derivatives* / Q000633:toxicity; D013961:Thyroid Gland / Q000187:drug effects; D013964:Thyroid Neoplasms / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D013965:Thyroidectomy; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
D004396:Coloring Agents; D005452:Fluoresceins; D005505:Food Coloring Agents; D009602:Nitrosamines; D014284:Triiodothyronine; D012395:Rose Bengal; C048837:Rose Bengal B; C012457:diisopropanolnitrosamine; D013972:Thyrotropin; D004923:Erythrosine; D013974:Thyroxine