The effects of beta-adrenergic drugs on the human sperm motility in vitro. I. The effects of propranolol and isoprenaline.
The investigations were carried out on 28 samples of human semen with asthenozoospermia. The experiments were performed in three groups of semen, adding to the first one 0.34 mM of propranolol and 0.025 mM of isoprenaline, to the second one the double dose and to the last one the fivefold dose of the drugs. The lowest concentrations of the drugs applied in our investigations result from the used therapeutic intravenous doses for people. The proportional content of moving sperma was evaluated under the phase-contrast microscope at the 5, 30, 60, 120 and 240 minute of the experiment. The addition of 0.34 mM of propranolol caused a decrease of the sperm motility but only after 60 minutes and for a short period of time. However, the addition of the double and the fivefold doses of the drug led to immediate and significant reduction of the sperm motility. The lowest dose of isoprenaline markedly increased the sperm activity in comparison with the control values but not earlier than 120 minutes after the addition. The further increase in dose showed no effect on the moving sperm percentage increase, merely accelerating this effect.
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D000328:Adult; D006801:Humans; D066298:In Vitro Techniques; D007248:Infertility, Male / Q000503:physiopathology; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D008858:Microscopy, Phase-Contrast; D011433:Propranolol / Q000494:pharmacology*; D011943:Receptors, Adrenergic, beta / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects*
D011943:Receptors, Adrenergic, beta; D011433:Propranolol; D007545:Isoproterenol