title: Distinct tissue specific mitochondrial creatine kinases from chicken brain and striated muscle with a conserved CK framework.
abstract: cDNA clones for chicken mitochondrial creatine kinase (Mi-CK) were isolated from a lambda gt11 leg muscle cDNA library and sequenced. The deduced amino acid sequence showed 6 blocks of extensive homologies with the cytosolic creatine kinases and contained twenty N-terminal amino acids, with characteristic features of part of a mitochondrial presequence. The mature enzyme contained 380 amino acids with a calculated Mr of 43'195. RNA hybridization analysis showed corresponding Mi-CK transcripts in cardiac and skeletal muscle, but not in brain RNA. Within the 30 N-terminal amino acids purified brain Mi-CK contained 10 changes with respect to cardiac Mi-CK. Thus multiple isoproteins of mitochondrial creatine kinases of brain and striated muscle are encoded by multiple mRNA's.
authors.affiliation: Institute for Cell Biology, Swiss Federal Institute of Technology, Zurich.
authors.forename: J P
authors.initials: JP
authors.lastname: Hossle
authors.forename: J
authors.initials: J
authors.lastname: Schlegel
authors.forename: G
authors.initials: G
authors.lastname: Wegmann
authors.forename: M
authors.initials: M
authors.lastname: Wyss
authors.forename: P
authors.initials: P
authors.lastname: Böhlen
authors.forename: H M
authors.initials: HM
authors.lastname: Eppenberger
authors.forename: T
authors.initials: T
authors.lastname: Wallimann
authors.forename: J C
authors.initials: JC
authors.lastname: Perriard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002645:Chickens; D003001:Cloning, Molecular; D003402:Creatine Kinase / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D007527:Isoenzymes / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology*; D008931:Mitochondria, Muscle / Q000201:enzymology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012984:Software
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D003402:Creatine Kinase; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI