Reduction of colonic carcinogenesis by wheat bran independent of fecal bile acid concentration.
It has been reported previously that populations with a decreased concentration of fecal bile acids have a lower incidence of colon cancer. We examined the importance of fecal bile acid dilution by wheat bran (WB) in inhibiting colonic tumorigenesis in an experimental animal model. Male F344 rats received oral doses of the colon carcinogen 1,2-dimethylhydrazine [CAS: 540-73-8] and were assigned randomly to groups fed one of four semipurified diets for 26 weeks. The diets were fiber-free (FF), 10% WB, FF + bile salts, or WB + bile salts. The amount of bile salts added was adjusted to produce a fecal bile acid concentration in the group fed WB + bile salts equal to that found in the FF groups. Fecal bile acid concentrations at 12 and 24 weeks in the WB + bile salts group were similar to those in the FF group. Gross and microscopic findings at necropsy revealed a reduced total number and multiplicity of colon tumors in both bran-fed groups. Although the fecal bile acid concentrations of the FF and WB + bile salts groups were equal, the latter showed a significant reduction in tumor yield.
Calvert
R J
RJ
Department of Surgery, University of Pennsylvania School of Medicine, Philadelphia 19104.
Klurfeld
D M
DM
Subramaniam
S
S
Vahouny
G V
GV
Kritchevsky
D
D
D019813:1,2-Dimethylhydrazine; D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis*; D001835:Body Weight / Q000187:drug effects; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D004043:Dietary Fiber; D004127:Dimethylhydrazines; D004435:Eating / Q000187:drug effects; D005243:Feces / Q000032:analysis*; D005769:Gastrointestinal Motility; D008297:Male; D008763:Methylhydrazines; D051381:Rats; D011916:Rats, Inbred F344
D001647:Bile Acids and Salts; D004043:Dietary Fiber; D004127:Dimethylhydrazines; D008763:Methylhydrazines; D019813:1,2-Dimethylhydrazine