Immunological variation in the 'common region' of the T antigens of polyoma virus.
Characterization of a series of mouse monoclonal antibodies (designated alpha Py C21 to 26) against the region common to the large, middle and small T antigens of polyoma virus has revealed immunological diversities among the N termini of these antigens. Four of the antibodies (alpha Py C21 to 24) appeared to recognize all the T antigens present in lytically infected cells, but two of them (alpha Py C25, 26) failed to immunoprecipitate small T antigen either from 35S- or 32P-labelled cells and recognized only a subset of middle and large T antigens. None of the antibodies recognized the protein kinase activity normally associated with middle T antigen or the 60K mol. wt. antigen-related protein observed in polyoma virus lytically infected or transformed cells. The possible immunodominance of the N termini of the early gene products (as well as VP1) in the natural host of polyoma virus and the observed antigenic heterogeneity are considered.
Street
A J
AJ
Imperial Cancer Research Fund, Lincoln's Inn Fields, London, U.K.
Griffin
B E
BE
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D001667:Binding, Competitive; D011232:Chemical Precipitation; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D011120:Polyomavirus / Q000276:immunology*; D013539:Simian virus 40 / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000957:Antigens, Viral, Tumor