Demonstration of human papillomavirus types 6 and 11 in juvenile laryngeal papillomatosis by in-situ DNA hybridization.
A study is reported in which an in situ hybridization technique for the demonstration of human papillomavirus (HPV) employing a biotin--streptavidin polyalkaline phosphatase complex has been successfully applied to formalin-fixed paraffin-processed tissue obtained from ten patients with juvenile laryngeal papillomatosis. In all cases, positive results were obtained for either HPV type 6 or 11. Normal vocal cord epithelium was negative.
Terry
R M
RM
Department of ENT Surgery, Bradford Royal Infirmary, U.K.
Lewis
F A
FA
Griffiths
S
S
Wells
M
M
Bird
C C
CC
D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007822:Laryngeal Neoplasms / Q000382:microbiology*; D008828:Microbiological Techniques; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000382:microbiology*; D027383:Papillomaviridae / Q000302:isolation & purification*
D004279:DNA, Viral