The role of proteases in 4-ipomeanol-induced enhancement of Sendai viral pneumonia in mice.
The ability of reconstituted, concentrated lyophilized lavage fluid to activate noninfectious Sendai virus (NISV) in vitro was examined. Lavage fluid was obtained from 4-ipomeanol (4-IP)-injured lungs at 1, 3, 5, 7, 9, and 13 days after treatment, and 1, 7, and 13 days after sham treatment (controls). Significantly higher viral titers were obtained using lavage fluid collected 1 day after 4-IP treatment. Higher protein concentrations were present in lavage fluid obtained at day 1 and 3 after 4-IP treatment. It is concluded that local viral-activating protease concentrations resulting from 4-IP-induced pulmonary injury is a likely microenvironmental modulator of paramyxoviral replication and can play an important role in paramyxoviral-induced lung injury.
Durham
S K
SK
Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca.
Dubovi
E A
EA
Castleman
W L
WL
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000201:enzymology*; D005260:Female; D008171:Lung Diseases / Q000139:chemically induced* / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D010222:Parainfluenza Virus 1, Human / Q000472:pathogenicity; D018184:Paramyxoviridae Infections / Q000382:microbiology* / Q000503:physiopathology; D010447:Peptide Hydrolases / Q000502:physiology*; D013729:Terpenes / Q000633:toxicity*; D014118:Toxins, Biological
D013729:Terpenes; D014118:Toxins, Biological; D010447:Peptide Hydrolases; C001470:4-ipomeanol