Mutual inactivation of valinomycin and protonophores by complex formation in liposomal membranes.
The stimulation presence of a protonophore [3,5-di(ter-butyl)-4-hydroxybenzylidenemalononitrile or carbonyl cyanide m-chlorophenylhydrazone] and valinomycin in a liposome suspension results in time-dependent inactivation of ion transport by both the protonophore and valinomycin. Correlation of the inactivation with spectrophotometric observations on the formation of a complex between the protonophore and valinomycin strongly suggests that the complex observed has no (or very low) activity for the transport of either H+ or K+. The stoichiometry of valinomycin and the protonophore in the inactive complex is shown to be 1:1.
Krishnamoorthy
G
G
Chemical Physics Group, Tata Institute of Fundamental Research, Bombay, India.
D001692:Biological Transport / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D004347:Drug Interactions; D006863:Hydrogen-Ion Concentration; D007476:Ionophores; D007700:Kinetics; D008081:Liposomes; D009570:Nitriles / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D011522:Protons; D013053:Spectrophotometry; D014475:Uncoupling Agents; D014634:Valinomycin / Q000494:pharmacology*
D007476:Ionophores; D008081:Liposomes; D009570:Nitriles; D011522:Protons; D014475:Uncoupling Agents; D014634:Valinomycin; C001784:SF 6847; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D011188:Potassium