Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA).
The development of three separate rapid ELISAs for detecting antibodies in host serum to three different viruses is described. These include: 1. A direct antigen assay using enzyme labelled anti-canine Ig for detecting antibodies to canine parvovirus, 2. A competitive ELISA using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. A competitive ELISA using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26. The utility and benefits of each of the three approaches is emphasized.
Winston
S
S
Allelix Inc., Mississauga, Ontario.
Fiscus
S
S
Hesterberg
L
L
Matsushita
T
T
Mildbrand
M
M
Porter
J
J
Teramoto
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D002371:Cat Diseases / Q000276:immunology; D002415:Cats / Q000276:immunology; D003332:Coronaviridae / Q000276:immunology*; D003333:Coronaviridae Infections / Q000276:immunology / Q000662:veterinary; D004283:Dog Diseases / Q000276:immunology; D004285:Dogs / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004859:Equine Infectious Anemia / Q000276:immunology; D006736:Horses / Q000276:immunology; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000276:immunology / Q000662:veterinary; D010538:Peritonitis / Q000276:immunology / Q000662:veterinary
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral