Studies with FtsA-LacZ protein fusions reveal FtsA located inner-outer membrane junctions.
Four FtsA-LacZ translational gene fusions were constructed using a mini-Mu transposon (MudII 1734). FtsA-LacZ fusions and FtsA protein that were radioactively labelled using maxicell technique fractionated identically into membranes and cytoplasm. The FtsA-LacZ fusion proteins were also localized in wild type dividing cells using beta-galactosidase activity. Fractions from a modified sucrose equilibrium gradient exhibited beta-galactosidase activity in fractions corresponding to outer membrane-heavy (OMH) and outer membrane light (OML). The data are consistent with a model in which FtsA protein is incorporated into septal adhesion sites associated with cell division.
Chon
Y
Y
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
Gayda
R
R
D001426:Bacterial Proteins / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase