Cell-cycle dependence of dolichyl phosphate biosynthesis.
The cell-cycle dependence of dolichyl phosphate biosynthesis has been investigated in mouse L-1210 cells fractionated by centrifugal elutriation. Dolichyl phosphate levels increased linearly through the cell cycle, reaching a value in late S phase twice that of early G1. The cell-cycle dependences of four dolichyl phosphate metabolizing enzymes have been measured: cis-prenyltransferase, CTP-dependent dolichol kinase, dolichyl phosphatase, and dolichyl pyrophosphatase. The kinase, the cis-prenyltransferase, and the pyrophosphatase showed cell-cycle variations, increasing through G1 to a maximum in S phase while the monophosphatase activity was cell-cycle independent. The rate of accumulation of dolichyl phosphate was not affected by growing the cells in mevalonolactone-supplemented media. The evidence presented is consistent with models in which either the cis-prenyltransferase or the kinase/phosphatase couple (or both) regulates the levels of dolichyl phosphate in the cell.
Adair
W L
WL
Department of Biochemistry, University of South Florida College of Medicine, Tampa 33612.
Cafmeyer
N
N
D000818:Animals; D002453:Cell Cycle; D004122:Dimethylallyltranstransferase / Q000378:metabolism*; D004288:Dolichol Phosphates / Q000096:biosynthesis*; D007700:Kinetics; D007939:Leukemia L1210 / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis*; D011755:Pyrophosphatases; D014166:Transferases / Q000378:metabolism*
D004288:Dolichol Phosphates; D011106:Polyisoprenyl Phosphates; D014166:Transferases; D004122:Dimethylallyltranstransferase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020087:dolichol kinase; D010744:Phosphoric Monoester Hydrolases; D011755:Pyrophosphatases; C023082:dolichyldiphosphatase