title: (Ca2+ + Mg2+)-dependent ATPase mRNA from smooth muscle sarcoplasmic reticulum differs from that in cardiac and fast skeletal muscles.
abstract: We have investigated some characteristics of the sarcoplasmic reticulum (Ca2+ + Mg2+)-dependent ATPase (Ca2+-ATPase) mRNA from smooth muscle using specific cDNA probes isolated from a rat heart cDNA library. RNA blot analysis has shown that the Ca2+-ATPase mRNA expressed in smooth muscle is identical in size to the cardiac mRNA but differs from that of fast skeletal muscle. S1 nuclease mapping has moreover shown that the cardiac and smooth muscle isoforms possess different 3'-end sequences. These results indicate that a distinct sarcoplasmic reticulum Ca2+-ATPase mRNA is present in smooth muscle.
authors.affiliation: INSERM U127, Hôpital Lariboisière, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: de la Bastie
authors.forename: C
authors.initials: C
authors.lastname: Wisnewsky
authors.forename: K
authors.initials: K
authors.lastname: Schwartz
authors.forename: A M
authors.initials: AM
authors.lastname: Lompré
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000235:genetics*; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics*; D009130:Muscle, Smooth / Q000201:enzymology* / Q000648:ultrastructure; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases