The effect of lipophilic amines on the growth of hepatitis A virus in Frp/3 cells.
The effect of lipophilic amines on hepatitis A virus infection in a monkey cell line (Frp/3 cells) was studied. Ammonium chloride, amantadine, methylamine and dansylcadaverine inhibited viral antigen synthesis when added to the cells at least one hour after the attachment step. Results obtained suggest that the HAV entry pathway in Frp/3 cells follows an endocytic route and that viral uncoating takes probably place in endosomes and/or lysosomes.
Superti
F
F
Istituto di Microbiologia, Università di Roma La Sapienza, Facoltà di Medicina e Chirurgia, Italy.
Seganti
L
L
Orsi
N
N
Divizia
M
M
Gabrieli
R
R
Panà
A
A
D000547:Amantadine / Q000494:pharmacology*; D000643:Ammonium Chloride / Q000494:pharmacology*; D000818:Animals; D002103:Cadaverine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D003959:Diamines; D004705:Endocytosis / Q000187:drug effects; D000882:Haplorhini; D006507:Hepatovirus / Q000187:drug effects / Q000502:physiology*; D008247:Lysosomes / Q000187:drug effects; D008744:Methylamines / Q000494:pharmacology*
D003959:Diamines; D008744:Methylamines; D000643:Ammonium Chloride; D000547:Amantadine; C027451:methylamine; C008542:monodansylcadaverine; D002103:Cadaverine