Restriction analysis and quantitative estimation of methylated bases of filamentous and unicellular cyanobacterial DNAs.
The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanation is substantiated by the content of N6-methyladenine and 5-methylcytosine in these genomes, which is high in comparison with that in other bacterial genomes. dcm- and dam-like methylases are present in the three strains (based on the restriction patterns obtained with the appropriate isoschizomeric enzymes). Their contribution to the overall content of methyladenine and methylcytosine in the genomes was calculated. Partial methylation of GATC sequences was observed in Anabaena DNA. In addition, the GATC methylation patterns might not have been random in the three cyanobacterial DNA preparations, as revealed by the appearance of discrete fragments (possibly of plasmid origin) withstanding cleavage by DpnI (which requires the presence of methyladenine in the GATC sequence).
Padhy
R N
RN
Microbiology and Genetics Unit, University of Louvain Medical School, Brussels, Belgium.
Hottat
F G
FG
Coene
M M
MM
Hoet
P P
PP
D044503:5-Methylcytosine; D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000235:genetics*; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D005796:Genes; D008745:Methylation
D044503:5-Methylcytosine; D000225:Adenine; D003596:Cytosine; D004247:DNA; D004262:DNA Restriction Enzymes; C000730567:6-methyladenine