A novel assay for 5'-nucleotidase using 1,N6-etheno-AMP as substrate, and comments on the properties of the reaction product, ethenoadenosine.
A novel assay for 5'-nucleotidase is described in which 1,N6-etheno-AMP is converted into ethenoadenosine. The product ethenoadenosine is neither a substrate for nor an inhibitor of adenosine deaminase. Ethenoadenosine appears to have little effect at adenosine receptors on adipose-tissue cells.
Jamal
Z
Z
Department of Biochemistry, University College London, U.K.
Afkham-Ebrahimi
A
A
Saggerson
E D
ED
D015720:5'-Nucleotidase; D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000243:Adenosine Deaminase / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D009708:Nucleotidases / Q000378:metabolism*; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011983:Receptors, Purinergic / Q000187:drug effects; D013053:Spectrophotometry
D011983:Receptors, Purinergic; D010660:Phenylisopropyladenosine; C030368:etheno-AMP; C007640:1,N(6)-ethenoadenosine; D000249:Adenosine Monophosphate; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D000241:Adenosine