The sequence of porcine chromogranin A messenger RNA demonstrates chromogranin A can serve as the precursor for the biologically active hormone, pancreastatin.
Specific oligonucleotide priming of double-stranded DNA has been employed to sequence a porcine chromogranin A adrenomedullary cDNA. Porcine chromogranin A is more than 80% identical to human, bovine, and rat chromogranin A at its deduced N- and C-termini. A 49-amino acid region of the porcine molecule is 59-71% homologous to corresponding areas of rat, bovine, and human chromogranin A, and identical to the amino acid sequence of porcine pancreastatin. The sequence is preceded by an arginine at the N-terminus and followed by a GKR sequence at the C-terminus. Thus, porcine chromogranin A can serve as the precursor for pancreastatin, a polypeptide capable of inhibiting insulin release from the endocrine pancreas and acid secretion from parietal cells of the gut.
Iacangelo
A L
AL
Unit on Molecular and Cellular Neurobiology, NIMH, Bethesda, MD 20892.
Fischer-Colbrie
R
R
Koller
K J
KJ
Brownstein
M J
MJ
Eiden
L E
LE
D000313:Adrenal Medulla / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D053379:Chromogranin A; D002864:Chromogranins / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010187:Pancreatic Hormones / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine
C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D010187:Pancreatic Hormones; D011498:Protein Precursors; D012333:RNA, Messenger; C506153:chromogranin A, rat; C050836:pancreastatin; D004262:DNA Restriction Enzymes