title: The bacteriophage lambda cohesive end site: isolation of spacing/substitution mutations that result in dependence on Escherichia coli integration host factor.
abstract: Substitution, insertion and deletion mutations have been constructed at the XmnI restriction site in cos lambda. The XmnI site is located between cosB, the site where terminase binds lambda DNA; and cosN, the site where terminase introduces staggered nicks to generate cohesive ends. Substitution mutations and deletion of a base pair (a -1 change) do not obviously affect lambda growth and DNA packaging. Changes of -2, +2 and -3 render lambda unable to grow on host cells lacking integration host factor (IHF). The -3 mutant has a reduced burst size in IHF+ cells, due to a defect in the initiation of packaging. A -7 deletion mutation is lethal. Models for the basis of these mutational effects are discussed.
authors.affiliation: Department of Microbiology, College of Medicine, University of Iowa, Iowa City 52242.
authors.forename: G
authors.initials: G
authors.lastname: Miller
authors.forename: M
authors.initials: M
authors.lastname: Feiss
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D034982:Integration Host Factors; D008242:Lysogeny; D008969:Molecular Sequence Data; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D004262:DNA Restriction Enzymes