Replication and packaging of choleraphage phi 149 DNA.
The intercellular replication of the circularly permuted DNA of choleraphage phi 149 involves a concatemeric DNA structure with a size equivalent to six genome lengths. The synthesis of both monomeric and concatemeric DNAs during replication of phi 149 occurred in the cytoplasm. The concatemers served as the substrate for the synthesis of mature phage DNA, which was eventually packaged by a headful mechanism starting from a unique pac site in the concatemeric DNA. Packaging of DNA into phage heads involved binding of concatemeric DNA to the cell membrane. A scheme involving sequential packaging of five headfuls proceeding in the counterclockwise direction from the pac site is proposed. After infection under high-phosphate conditions, the concatemeric DNA intermediates were not formed, although synthesis of monomeric molecules was unaffected.
Chowdhury
R
R
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
Ray
A
A
Ray
P
P
Das
J
J
D001426:Bacterial Proteins; D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000096:biosynthesis*; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D009690:Nucleic Acid Conformation; D010710:Phosphates / Q000494:pharmacology; D014734:Vibrio cholerae; D014779:Virus Replication
D001426:Bacterial Proteins; D003470:Culture Media; D004279:DNA, Viral; D010710:Phosphates; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific