[A new chromatographic method for the preparative isolation of phosphoinositides and other anionic phospholipids].
A new chromatographic procedure for preparative isolation of mono-, di- and triphosphoinositides and other anionic phospholipids with the use of adsorbents containing primary amino groups is described. Sorbents with immobilized neomycin, L-lysine and aminoalkyl groups were tested. Conditions for isolation of chromatographically pure phospholipids of separate classes on the above sorbents were developed. Isolation of polyphosphoinositides on the amino sorbents represents a new type of chromatography involving bioaffinity and ion-exchange interaction.
Kliashchitskiĭ
B A
BA
Mezhova
I V
IV
Starkova
E P
EP
Krasnopol'skiĭ
Iu M
IuM
Sennikov
G A
GA
D000327:Adsorption; D000596:Amino Acids; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D007202:Indicators and Reagents; D008563:Membrane Lipids / Q000302:isolation & purification*; D009355:Neomycin; D010716:Phosphatidylinositols / Q000302:isolation & purification*; D010743:Phospholipids / Q000302:isolation & purification*
D000596:Amino Acids; D007202:Indicators and Reagents; D008563:Membrane Lipids; D010716:Phosphatidylinositols; D010743:Phospholipids; D009355:Neomycin
Novyĭ khromatograficheskiĭ metod preparativnogo vydeleniia fosfoinozitidov i drugikh anionnykh fosfolipidov.