[Homologous and heterologous transcription of the Cry+-plasmid in Bacillus thuringiensis].
The possibility of homologous and heterologous transception of Cry+ plasmids in Bacillus thuringiensis is demonstrated. Cry+ plasmids from crystal bearing strain of Bacillus thuringiensis were transferred into acrystalline strain belonging to H5 serotype by mutual incubation. The donor strain was previously marked by the transmissive plasmid pAM beta 1 coding for erythromycin and lincomycin resistance. The transcipients having acquired the ability to synthesize delta-endotoxin were referred to H5 serotype due to their phenotype. By analogous method Cry+ plasmid was transferred from Bacillus thuringiensis to Bacillus cereus. Bacillus cereus strain GP7 was used as a recipient strain resistant to tetracycline. The presence of delta-endotoxin in transcipients was confirmed by bioprobes and immunoenzyme assay. To prove the transfer of Cry+ plasmid the plasmid profiles of the parent strains and transcipients have been analyzed. The formation of cellular contacts during mutual incubation of Bacillus thuringiensis and Bacillus cereus strains was demonstrated by electron microscopic study of ultrafine cuts.
Smirnova
T A
TA
Bogdanova
T L
TL
Gal'perin
M Iu
MIu
Grigor'eva
T M
TM
Azizbekian
R R
RR
D001413:Bacillus thuringiensis / Q000235:genetics* / Q000648:ultrastructure; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003460:Crystallization; D004731:Endotoxins / Q000096:biosynthesis / Q000235:genetics*; D005822:Genetic Vectors; D006460:Hemolysin Proteins; D010572:Pest Control, Biological; D010957:Plasmids
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
Gomologichnaia i geterologichnaia transtseptsiia Cry+-plazmid Bacillus thuringiensis.