[Determination of berberine and palmatine in cortex phellodendron and Chinese patent medicines by HPLC].
Berberine (1) and palmatine (2) in cortex phellodendron and Chinese patent medicines were determined by HPLC. The optimal composition of mobile phase CH3COOEt-HCOOH-EtOH (15:3:2) for HPLC separation of berberine and palmatine was successfully determined by using window diagram technique. Detection wavelength was 346nm. Flow rate: 1.5 ml/min. Calibration graphs for 1 and 2 were rectilinear for 0.06-0.32 micrograms and 0.12-0.32 micrograms respectively. The average recoveries of the two corresponding components were found to be over 96% and their coefficients of variation were below 5%.
Wang
Y M
YM
Zhao
L B
LB
Lin
S L
SL
Dong
S S
SS
An
D K
DK
D001599:Berberine / Q000032:analysis*; D001600:Berberine Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*
D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D001599:Berberine; C005413:palmatine