Electrophoretic forms of protein kinases during differentiation of murine erythroleukaemic cells.
The different forms of cAMP-dependent, cGMP-dependent and cyclic nucleotide-independent protein kinases were studied in relation to hexamethylene bisacetamide (HMBA)-induced differentiation of murine erythroleukaemic cells, using a nondenaturing polyacrylamide gel electrophoresis system. Several forms of histone-dependent kinases were detected and there was a definite change in the relative proportions of the two major components and of minor components in cells treated with HMBA, as compared with untreated controls. The faster-moving of the two major forms, which increased on HMBA stimulation, appeared to be both cAMP and cGMP dependent; the slower moving form, which decreased with HMBA treatment, showed little dependence on either cAMP or cGMP.
Hammond
K D
KD
Sprott
S
S
Savage
N
N
D000081:Acetamides / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology* / Q000473:pathology; D011494:Protein Kinases / Q000032:analysis*
D000081:Acetamides; D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP; C014026:hexamethylene bisacetamide