Clonal rearrangement for immunoglobulin and T-cell receptor genes in systemic Castleman's disease. Association with Epstein-Barr virus.
Castleman's disease is a morphologically and clinically heterogeneous lymphoproliferative disorder. Both a localized benign variant and an aggressive form with systemic manifestations have been described. To investigate the differences between these variants of Castleman's disease, the authors analyzed lymph node DNA from 4 patients with the localized type and 4 with the systemic type of Castleman's disease for immunoglobulin and T-cell receptor gene rearrangements. The role of Epstein-Barr virus (EBV) and cytomegalovirus (CMV) was also studied by viral genomic DNA probes. They detected clonal rearrangements in 3 of the 4 patients with the systemic variant of Castleman's; no patients with localized disease had rearrangements. Copies of EBV genome were also detected in 2 of the 3 patients with clonal rearrangements. These results suggest that systemic Castleman's disease is a disorder distinct from the classical localized variant in that it may evolve into a clonal lymphoproliferation.
Hanson
C A
CA
Department of laboratory Medicine and Pathology, University of Minnesota Hospital Clinic, Minneapolis.
Frizzera
G
G
Patton
D F
DF
Peterson
B A
BA
McClain
K L
KL
Gajl-Peczalska
K J
KJ
Kersey
J H
JH
D000293:Adolescent; D000328:Adult; D000368:Aged; D005871:Castleman Disease / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D005796:Genes; D005803:Genes, Immunoglobulin; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D008198:Lymph Nodes / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell