Cloning and characterization of Streptococcus mutans LM7 plasmid pAM7.
The 5.6-kilobase-pair cryptic plasmid, pAM7, of Streptococcus mutans LM7 was cloned into Escherichia coli plasmids or a shuttle plasmid to examine whether the plasmid encodes bacteriocin. Plasmid pAM7 encoded proteins with molecular weights of 30,000, 22,000, and 12,000, but none of them appeared to be bacteriocin.
Noji
S
S
Department of Biochemistry, Okayama University Dental School, Japan.
Date
S
S
Abiko
Y
Y
Takiguchi
H
H
Taniguchi
S
S
D001426:Bacterial Proteins / Q000235:genetics*; D001430:Bacteriocins / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008970:Molecular Weight; D010957:Plasmids; D014176:Protein Biosynthesis; D013295:Streptococcus mutans / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D001430:Bacteriocins; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific