[Determination of mercapturic acid in urine samples for the monitoring of occupational exposure to xenobiotics].
The determination of mercapturic acids in urine samples of employees exposed to carcinogenic substances requires highly sensitive and specific methods such as GC/MS or HPLC/Fluorimetry. We applied GC/MS to the analysis of thiodiglycolic acid, which is an indicator metabolite of vinyl chloride exposure and of S-phenylmercapturic acid which can be used to control benzene exposure. S-cyanoethylmercapturic acid appears as a degradation product during acrylonitrile metabolism. This metabolite can be determined by HPLC combined with a sulfur specific fluorimetric detector. This procedure which is of general use offers the possibility to improve the biological monitoring in case of exposures to carcinogenic substances.
Norpoth
K
K
Müller
G
G
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D002273:Carcinogens / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013864:Thioglycolates / Q000652:urine; D015262:Xenobiotics
D002273:Carcinogens; D013864:Thioglycolates; D015262:Xenobiotics; C053003:S-phenyl-N-acetylcysteine; C010518:thiodiacetic acid; C025645:N-acetyl-S-(2-cyanoethyl)cysteine; D000111:Acetylcysteine
Bestimmung von Merkaptursäuren in Harnproben zur Kontrolle beruflicher Fremdstoff-Expositionen.