Neutralization of diverse HIV-1 strains by monoclonal antibodies raised against a gp41 synthetic peptide.
Three IgM monoclonal antibodies raised against synthetic peptide analogs of a hydrophilic region of the gp41 transmembrane env protein of HIV-1 neutralize different HIV-1 isolates but not HIV-2 isolates, as determined by HIV titration and by syncytial inhibition assays. VSV (HIV-1) pseudotypes, however, were not neutralized, indicating that gp41 was not accessible to these antibodies on the pseudotype particles. The antibodies affect early steps in adsorption and penetration of HIV-1.
Dalgleish
A G
AG
MRC Clinical Research Centre, Harrow, United Kingdom.
Chanh
T C
TC
Kennedy
R C
RC
Kanda
P
P
Clapham
P R
PR
Weiss
R A
RA
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D006678:HIV / Q000145:classification / Q000276:immunology*; D015488:HIV Antigens; D015700:HIV Envelope Protein gp41; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D012191:Retroviridae Proteins / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D015488:HIV Antigens; D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins