Comparison of high performance liquid chromatographic and microbiological methods for determination of itraconazole.
A reversed-phase high-performance liquid chromatographic (HPLC) method, with internal standard quantification, is described for the analysis of itraconazole in human serum. No interference was encountered from over 60 drugs tested. The standard curve was linear from 10 to 10,000 micrograms/l. The detection limit of the method was 10 micrograms/l, with coefficients of variation from 2.2 to 7.8% over a range of itraconazole concentrations from 20 to 1600 micrograms/l. An agar diffusion method is also described with a lowest reproducible limit of 100 micrograms/l. This method had coefficients of variation from 11.0 to 17.1% over a range of itraconazole concentrations from 100 to 1600 micrograms/l. Comparison of the methods showed that HPLC gave much lower values of itraconazole concentrations in patient serum samples than did the microbiological method.
Warnock
D W
DW
Department of Microbiology, Bristol Royal Infirmary, UK.
Turner
A
A
Burke
J
J
D000935:Antifungal Agents / Q000097:blood*; D001681:Biological Assay; D002176:Candida albicans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D017964:Itraconazole; D007654:Ketoconazole / Q000031:analogs & derivatives* / Q000097:blood
D000935:Antifungal Agents; D007202:Indicators and Reagents; D017964:Itraconazole; D007654:Ketoconazole