The specificity of thiourea, dimethylthiourea and dimethyl sulphoxide as scavengers of hydroxyl radicals. Their protection of alpha 1-antiproteinase against inactivation by hypochlorous acid.
Thiourea and dimethylthiourea are powerful scavengers of hydroxyl radicals (.OH), and dimethylthiourea has been used to test the involvement of .OH in several animal models of human disease. It is shown that both thiourea and dimethylthiourea are scavengers of HOCl, a powerful oxidant produced by neutrophil myeloperoxidase. Hence the ability of dimethylthiourea to protect against neutrophil-mediated tissue damage cannot be used as evidence for a role of .OH in causing such damage. Dimethyl sulphoxide also reacts with HOCl, but at a rate that is probably too low to be biologically significant at dimethyl sulphoxide concentrations up to 10 mM. Neither mannitol nor desferrioxamine, at the concentrations normally used in radical-generating systems, appears to react with HOCl.
Wasil
M
M
Department of Thoracic Medicine, Faculty of Clinical Medicine, King's College (KQC), Denmark Hill, London, U.K.
Halliwell
B
B
Grootveld
M
M
Moorhouse
C P
CP
Hutchison
D C
DC
Baum
H
H
D003676:Deferoxamine / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005609:Free Radicals; D006997:Hypochlorous Acid / Q000494:pharmacology*; D008353:Mannitol / Q000494:pharmacology; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000515:alpha 1-Antitrypsin
D005609:Free Radicals; D000515:alpha 1-Antitrypsin; D008353:Mannitol; D006997:Hypochlorous Acid; C038983:1,3-dimethylthiourea; D010196:Pancreatic Elastase; D013890:Thiourea; D003676:Deferoxamine; D004121:Dimethyl Sulfoxide