How to build an inexpensive cyclotherm instrument for automated polymerase chain reaction.
The Cyclotherm instrument is a functionally fully equivalent but inexpensive alternative to commercial instruments for automated polymerase chain reaction (PCR). It can be rebuilt under conditions of a biochemical laboratory for less than +1000. A Peltier element is used for heating and cooling of the reaction vials and the temperature and timing of the PCR cycles are controlled by a BASIC program in a SHARP PC 1600 low cost computer.
Collasius
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Mildred-Scheel-Labor für Krebszellforschung, Max-Planck-Institut, Martinsried, Federal Republic of Germany.
Falk
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Ciesler
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Valet
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D003199:Computer Systems; D004259:DNA-Directed DNA Polymerase; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012984:Software
D004259:DNA-Directed DNA Polymerase