title: The development and standardization of an enzyme-linked immunosorbent assay for the detection of antibodies to bovine herpesvirus 2.
abstract: A single dilution enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bovine herpesvirus 2 has been developed, standardized and compared with the virus neutralization test. The results of the two tests correlated well. A positive/negative threshold was established for the ELISA. The ELISA was reproducible, sensitive, rapid and specific.
authors.affiliation: Central Veterinary Laboratory, Ministry of Agriculture, Fisheries and Food, Weybridge, Surrey, UK.
authors.forename: S E
authors.initials: SE
authors.lastname: Bushnell
authors.forename: S
authors.initials: S
authors.lastname: Edwards
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006564:Herpesviridae / Q000276:immunology*; D001911:Herpesvirus 2, Bovine / Q000276:immunology*; D009500:Neutralization Tests
chemical_list: D000914:Antibodies, Viral