Construction of an excretion vector and extracellular production of human growth hormone from Escherichia coli.
A new excretion vector, pEAP8, was constructed to develop an excretion system for Escherichia coli. This plasmid, derived from pEAP37, carried the weakly activated kil gene of plasmid pMB9 [Kobayashi et al., J. Bacteriol. 166 (1986) 728-732] and the penicillinase promoter and signal region of an alkalophilic Bacillus sp. to excrete foreign gene products. A gene for human growth hormone (hGH) was joined to this signal sequence through the HindIII site. The recombinant plasmid p8hGH1 thus constructed, was introduced into E. coli. The hybrid protein which was produced in E. coli carrying p8hGH1 was processed during transport through the inner membrane, with the mature hGH being excreted into the medium through the outer membrane which was made permeable by the action of the kil gene. The N-terminal amino acid sequence and the biological activity of the extracellular hGH were consistent with those of the authentic hGH.
Kato
C
C
Central Research Laboratories, ZERIA Pharmaceutical Co., Ltd., Saitama, Japan.
Kobayashi
T
T
Kudo
T
T
Furusato
T
T
Murakami
Y
Y
Tanaka
T
T
Baba
H
H
Oishi
T
T
Ohtsuka
E
E
Ikehara
M
M
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003001:Cloning, Molecular / Q000379:methods; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D007016:Hypophysectomy; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D013006:Growth Hormone; D004262:DNA Restriction Enzymes