Protein kinase C and parietal cell function.
Ca2+-phospholipid dependent protein kinase activity and the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on H+ secretion was studied in guinea-pig parietal cells. In resting parietal cells PK-C activity was distributed almost equally in both the cytosolic and particulate cell fraction. Exposure of the cells to TPA resulted in a loss of cytosolic PK-C activity which was not accompanied by a concomitant increase of particulate activity. Furthermore TPA inhibits the K+/H+-ATPase and dissipates H+ gradient in intact gastric vesicles by a protonophoric action. The role of these biochemical events for the antisecretory action of TPA found in the intact parietal cell are discussed.
Beil
W
W
Abteilung Allgemeine Pharmakologie, Medizinische Hochschule Hannover, F.R.G.
Mannschedel
W
W
Sewing
K F
KF
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D006168:Guinea Pigs; D017506:H(+)-K(+)-Exchanging ATPase; D010295:Parietal Cells, Gastric / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D011493:Protein Kinase C / Q000032:analysis* / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D011493:Protein Kinase C; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium