Characterization of a new human papillomavirus (HPV 41) from disseminated warts and detection of its DNA in some skin carcinomas.
A new human papillomavirus type (HPV 41), distantly related to known HPV prototypes, was isolated from a patient with disseminated facial, peri-anal and foot warts (epidermodysplasia verruciformis was not diagnosed). The viral DNA was molecularly cloned in 2 BamH1 restriction fragments with sizes of 6.5 kb and 0.98 kb, respectively. The classification of this cloned DNA as a new type is based on the degree of cross-hybridization with 40 HPV types under conditions of varying stringency. A total of 106 benign and malignant skin lesions, as well as 71 malignant tumours of different origins, were screened for the presence of HPV-related sequences. In 2 out of 10 squamous-cell carcinomas and in 1 of 3 arsenic keratoses HPV 41 DNA sequences could be detected.
Grimmel
M
M
Deutsches Krebsforschungszentrum, Heidelberg.
de Villiers
E M
EM
Neumann
C
C
Pawlita
M
M
zur Hausen
H
H
D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009369:Neoplasms / Q000382:microbiology / Q000473:pathology; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D012867:Skin / Q000166:cytology / Q000473:pathology; D012878:Skin Neoplasms / Q000382:microbiology* / Q000473:pathology; D014860:Warts / Q000382:microbiology / Q000473:pathology
D004279:DNA, Viral; D004262:DNA Restriction Enzymes