title: The long terminal repeat of feline endogenous RD-114 retroviral DNAs: analysis of transcription regulatory activity and nucleotide sequence.
abstract: Six cloned 5' long terminal repeat (LTR) and adjoining cellular DNA regions of partially deleted feline endogenous RD-114 proviral loci were linked to the chloramphenicol acetyltransferase (CAT) gene and assayed for their ability to promote transient CAT expression. One endogenous LTR (clone CRL-3) and the LTR from an infectious RD-114 provirus, EX-LTR, were capable of actively expressing the CAT gene. DNA sequence comparison of these LTRs with an inactive endogenous LTR (CR-1) revealed extensive homology in all regions except in the 5' half of U3. The homologous portion contained transcriptional regulatory sequences including CAT, TATA, polyadenylation signal boxes and an octamer enhancer, which is rarely seen in retroviruses. Variations in the 5' half of U3 were primarily due to insertions and deletions. A major difference was in number of copies and integrity of tandemly repeated sequences. EX-LTR contained two pairs of tandem direct repeats, while the two endogenous LTRs contained different deletions of repeated sequences. DNA sequence data also revealed that the primer binding site for RD-114 loci was complementary to a glycine tRNA isotype, the use of which is distinct from any other known retrovirus. An analysis of the steady state RNA levels in T-lymphoid cell lines showed that at least three different incomplete proviral transcripts and their spliced products made up the majority of expressed RD-114 mRNA, and further demonstrated that partially deleted proviral loci have the potential to be transcriptionally vigorous in certain feline cell types.
authors.affiliation: Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033.
authors.forename: D A
authors.initials: DA
authors.lastname: Spodick
authors.forename: A K
authors.initials: AK
authors.lastname: Ghosh
authors.forename: S
authors.initials: S
authors.lastname: Parimoo
authors.forename: P
authors.initials: P
authors.lastname: Roy-Burman
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase; D004279:DNA, Viral / Q000235:genetics; D005809:Genes, Regulator; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012353:RNA, Transfer, Gly / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D007143:Immunoglobulin Heavy Chains; D012353:RNA, Transfer, Gly; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase