title: Comparison of the phosphatases of Lysobacter enzymogenes with those of related bacteria.
abstract: Lysobacter enzymogenes ATCC 29487 (UASM 495) produces an outer-membrane-associated phosphatase and an excreted phosphatase. The cell-associated enzyme was compared to phosphatases of nine other Gram-negative gliding bacteria and to that of Escherichia coli. The other three species of the genus Lysobacter also produce a particulate, cell-associated phosphatase. Antiserum prepared against the phosphatase from the outer membrane of L. enzymogenes effectively precipitated the phosphatases of two other L. enzymogenes strains and the enzymes of L. antibioticus, L. brunescens and L. gummosus. Some inhibition of the enzyme by the antiserum also was observed. No significant reaction could be detected between the antiserum and the cell-associated phosphatases of species of Cytophaga johnsonae, 'C. compacta', Myxococcus xanthus, E. coli and the excreted phosphatase of L. enzymogenes. The results indicate that the four species of the genus Lysobacter are closely related despite their physiological differences and that the outer-membrane-associated phosphatases of these organisms have different structural characteristics than the phosphatases of the other Gram-negative bacteria that were used. Furthermore, differences in the amino acid compositions of the cell-associated and the excreted phosphatase of L. enzymogenes confirm the immunological results and are in agreement with the physical and chemical differences noted between the two enzymes.
authors.affiliation: Department of Microbiology, University of Alberta, Edmonton, Canada.
authors.forename: R G
authors.initials: RG
authors.lastname: von Tigerstrom
authors.forename: S
authors.initials: S
authors.lastname: Stelmaschuk
mesh_terms: D000596:Amino Acids / Q000032:analysis; D041964:Cytophagaceae / Q000201:enzymology*; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D007106:Immune Sera; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*
chemical_list: D000596:Amino Acids; D007106:Immune Sera; D010744:Phosphoric Monoester Hydrolases