Developmental changes in 5-hydroxytryptamine immunoreactivity of sympathetic cells.
5-Hydroxytryptamine was demonstrated immunohistochemically in developing sympathetic chain ganglia, adrenal medullae and retroperitoneal paraganglionic tissue. Initially, all catecholamine-synthesizing cells were immunoreactive to 5-hydroxytryptamine, but during the maturation some sympathetic cell types lose this property. In the ganglia, small intensely fluorescent cells retain their 5-hydroxytryptamine content throughout maturation. This property is expressed transitionally in some neonatal principal neurons, but can be triggered even in adult neurons, e.g. after loading with L-tryptophan. In the adrenal medulla, immunoreactivity to 5-hydroxytryptamine is carried over to the adrenaline-synthesizing cells, while in the main retroperitoneal paraganglion it is observed in both adrenaline and noradrenaline cells. These results suggest that initial expression of 5-hydroxytryptamine immunoreactivity is coupled to the onset of catecholamine synthesis, but is regulated differently in each mature sympathetic organ.
Soinila
S
S
Mark O. Hatfield Marine Science Center, Oregon State University, Newport 97365.
Ahonen
M
M
Lahtinen
T
T
Häppölä
O
O
D000313:Adrenal Medulla / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D005314:Embryonic and Fetal Development; D005726:Ganglia, Parasympathetic / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D005728:Ganglia, Sympathetic / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D009414:Nerve Growth Factors / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D009414:Nerve Growth Factors; D012701:Serotonin