Characterization of single-copy probe from vicinity of centromere of human chromosome 1.
Human DNA sequences in the human-hamster somatic cell hybrid XJM12.1.3 exist in the form of a minichromosome including the centromere of human chromosome 1. We describe the cloning of XJM12.1.3 DNA into the lambda vector EMBL3, the identification of minichromosome DNA-containing recombinants by hybridization with human sequences, and the characterization of one recombinant as a specific and unique probe for a region close to the centromere of human chromosome 1. This probe and others isolated from the minichromosome DNA are being developed to permit molecular access to a human centromere and its functional sequences.
Solus
J
J
Department of Biology, University of California, San Diego, La Jolla 92093.
Jacquemin-Sablon
A
A
Carine
K
K
Waltzer
E
E
Scheffler
I E
IE
D000818:Animals; D002503:Centromere; D002875:Chromosomes; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D006224:Cricetinae; D004262:DNA Restriction Enzymes; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004262:DNA Restriction Enzymes