Characteristics of the porcine kidney cell line IB-RS-2 clone D10 (IB-RS-2 D10) which is free of hog cholera virus.
A Brazilian stock of clone C17 of the IB-RS-2 porcine kidney cell line which was contaminated with hog cholera virus (HCV) was cloned. One clone designated IB-RS-2 D10 was determined to be free of HCV, 20 other viruses, and Mycoplasma. IB-RS-2 D10 cells possessed the same viral susceptibility pattern as the contaminated parent cells to the viruses of foot-and-mouth disease, swine vesicular disease, vesicular exanthema of swine, transmissible gastroenteritis, and several other viruses. The IB-RS-2 D10 cells had a median chromosome count of 34, were morphologically epithelioid cells, and were resistant to HCV infection. Freedom from HCV affords advantages for vaccine production and avoids laboratory contamination.
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United States Department of Agriculture, Foreign Animal Disease Diagnostic Laboratory, Greenport, New York 11944.
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Llewellyn
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D000818:Animals; D005537:Aphthovirus / Q000254:growth & development; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000254:growth & development / Q000302:isolation & purification; D002999:Clone Cells; D001908:Diarrhea Viruses, Bovine Viral / Q000254:growth & development / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D007621:Karyotyping; D007668:Kidney / Q000382:microbiology*; D009174:Mycoplasma / Q000302:isolation & purification; D013552:Swine