Studies on the attenuation of the Sabin type 3 oral polio vaccine.
The genetic basis of attenuation of the poliovirus type 3 vaccine strain P3/Leon 12a1b has been investigated by comparing the nucleotide sequence of this strain with that of its neurovirulent progenitor P3/Leon/37 and by constructing recombinants between these two viruses using infectious cDNAs. Preliminary results suggest that attenuation is caused by just two point mutations, one occurring in the 5' non-coding region and the other causing an amino acid change in coat protein VP3.
Almond
J W
JW
Department of Microbiology, University of Reading, U.K.
Westrop
G D
GD
Evans
D M
DM
Dunn
G
G
Minor
P D
PD
Magrath
D
D
Schild
G C
GC
D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D009154:Mutation; D010641:Phenotype; D011051:Poliomyelitis / Q000517:prevention & control*; D017955:Poliovirus / Q000235:genetics* / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral; D011995:Recombination, Genetic; D014613:Vaccines, Attenuated; D014774:Virulence
D004279:DNA, Viral; D011055:Poliovirus Vaccine, Oral; D014613:Vaccines, Attenuated