title: Cytotoxicity of respirable dusts from industrial minerals: comparison of two naturally occurring and two man-made silicates.
abstract: The membranolytic and cytotoxic properties of two naturally occurring (chrysotile asbestos; attapulgite clay) and two man-made (Fiberfrax, an aluminium-silicate, and xonotlite, a calcium silicate) industrial minerals were compared. "Short" fiber fractions of chrysotile and Fiberfrax were obtained by sedimentation in demineralized water, while the attapulgite and xonotlite samples were used as obtained. The aluminium silicate fibers were found to be non- hemolytic, while for the other three silicates, chrysotile had the strongest hemolysis potential, followed very closely by xonotlite; attapulgite was less hemolytic than the former two silicates, but was nevertheless highly hemolytic to the rat erythrocytes. Using rat pulmonary alveolar macrophages, the in vitro cytotoxicity assays showed that with fresh cell monolayers, all four silicates were equivalent in causing cell damages at a dose of 250 micrograms; at a lower dose (50 micrograms), the intensity of the cytotoxic effect was in the decreasing order: Fiberfrax greater than attapulgite greater than chrysotile greater than xonotlite. With one day-old cultured cell monolayers, a dose of 250 micrograms of the silicates fibers was less cytotoxic, with the exception of the attapulgite fibers which remained essentially as cytotoxic as with the fresh cell monolayers. The reduced cytotoxic response was especially noticeable with the chrysotile fibers. At 50 micrograms, the cytotoxicity scale of the mineral dusts with one day-old cell monolayers was essentially the same as the one obtained with the fresh cell monolayers, that is: Fiberfrax approximately equal to attapulgite greater than chrysotile greater than or equal to xonotlite. Overall, these in vitro tests imply: 1) that all four industrial silicates tested can be considered to be "biologically active"; 2) that on the basis of their different reactivities with the two types of cell culture conditions used, their biological reactivity in vivo might be quite distinct. This might be especially true for at least the chrysotile, attapulgite and xonotlite short fibers, considering that these three types of silicate dusts have very similar dimensions. Moreover, for the chrysotile and attapulgite samples, fiber numbers is probably not an important factor, since the density of the two silicates is roughly the same. The unaltered cytotoxic responses of the American attapulgite fibers in the two macrophage assays correlate well with the fact that short attapulgite fibers seems to have a stronger in vivo reactivity than short chrysotile fibers. In fact, it might be the strongest of the four industrial silicates tested.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Département de biologie, Faculté des sciences, Université de Sherbrooke, Québec, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Nadeau
authors.forename: L
authors.initials: L
authors.lastname: Fouquette-Couture
authors.forename: D
authors.initials: D
authors.lastname: Paradis
authors.forename: J
authors.initials: J
authors.lastname: Khorami
authors.forename: D
authors.initials: D
authors.lastname: Lane
authors.forename: J
authors.initials: J
authors.lastname: Dunnigan
mesh_terms: D000818:Animals; D001194:Asbestos / Q000633:toxicity; D017632:Asbestos, Serpentine; D017610:Calcium Compounds; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004391:Dust / Q000009:adverse effects*; D006461:Hemolysis / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D008274:Magnesium / Q000633:toxicity; D017616:Magnesium Compounds; D010316:Particle Size; D011650:Pulmonary Alveoli / Q000166:cytology; D051381:Rats; D017640:Silicates; D012824:Silicic Acid / Q000633:toxicity; D012825:Silicon / Q000633:toxicity; D017655:Silicon Compounds; D012822:Silicon Dioxide / Q000633:toxicity*
chemical_list: D017632:Asbestos, Serpentine; D017610:Calcium Compounds; D004391:Dust; D007773:Lactates; D017616:Magnesium Compounds; D017640:Silicates; D017655:Silicon Compounds; D001194:Asbestos; D012824:Silicic Acid; D012822:Silicon Dioxide; D007770:L-Lactate Dehydrogenase; D008274:Magnesium; C031293:calcium silicate; C026325:attapulgite; D012825:Silicon