Proteins are cointernalized with virion particles during early infection.
Mammalian cells infected with enveloped or naked animal viruses become permeabilized to several proteins. The entry of alpha-sarcin, horseradish peroxidase, and luciferase is greatly increased during the early stages of viral infection. This process is promoted by uv-inactivated SFV, but not by heat-inactivated virions, suggesting that the process does not require viral gene expression. The entry of alpha-sarcin has been monitored both by its effects on protein synthesis and by indirect immunofluorescence. Increased entry of alpha-sarcin and luciferase is clearly observed in animal virus-infected cells by fluorescence microscopy. Chloroquine blocks the coentry of alpha-sarcin with enveloped, but not with naked, viruses. These results have implications to elucidate the mechanisms involved in virus entry.
Otero
M J
MJ
Carrasco
L
L
D000260:Adenoviruses, Human; D000818:Animals; D002463:Cell Membrane Permeability; D002738:Chloroquine / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects; D004722:Endoribonucleases; D005656:Fungal Proteins / Q000378:metabolism / Q000494:pharmacology; D006367:HeLa Cells / Q000502:physiology*; D006735:Horseradish Peroxidase / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D017955:Poliovirus; D014176:Protein Biosynthesis / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D012672:Semliki forest virus; D014709:Vero Cells / Q000502:physiology*; D014721:Vesicular stomatitis Indiana virus; D014771:Virion
D005656:Fungal Proteins; D011506:Proteins; C014801:alpha-sarcin; D002738:Chloroquine; D006735:Horseradish Peroxidase; D008156:Luciferases; D004722:Endoribonucleases