Tissue distribution of calcium-activated neutral proteinases in rat.
A simple separation method involving a minimum number of essential steps was established to separate the two kinds of calcium-activated neutral proteinase (CANP) activity from each other and from endogenous CANP inhibitor activity. Determination of CANP activity in rat tissues using this method revealed a ubiquitous distribution of two CANPs. The level of CANP activity, however, differs between tissues. Low-calcium-requiring CANP (microCANP) is plentiful in spleen and kidney, while high-calcium-requiring CANP (mCANP) is plentiful in lung and brain. In all of the tissues examined, mCANP activity predominates over microCANP activity.
Kawashima
S
S
Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
Hayashi
M
M
Saito
Y
Y
Kasai
Y
Y
Imahori
K
K
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002154:Calpain / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D007668:Kidney / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D013154:Spleen / Q000201:enzymology; D014018:Tissue Distribution
D002154:Calpain; D002118:Calcium