Expression of an early Epstein-Barr virus antigen (EA-D) in E. coli. Brief report.
The 1.34 kb BcII-BgIII-fragment of the BamHI-M region of Epstein-Barr virus genome, comprising the complete BMRF1 open reading frame, was cloned into the tryptophan regulated E. coli expression vector pATH1. The resulting fusion protein, having a molecular weight of 80 kd, is recognized not only by anti-early antigen (EA)-positive human sera but also by the monoclonal antibody R3 directed against the diffuse component of EA (EA-D). A possible use for this fusion protein as an indicator protein in diagnosis of IgA antibodies against EA-D is presented.
Roeckel
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Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Federal Republic of Germany.
Boos
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Mueller-Lantzsch
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D000956:Antigens, Viral / Q000235:genetics*; D002051:Burkitt Lymphoma / Q000382:microbiology; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D014158:Transcription, Genetic
D000956:Antigens, Viral; C033305:Epstein-Barr virus early antigen; D004262:DNA Restriction Enzymes