Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 100% entrapment of the DNA, DOTMA facilitates fusion of the complex with the plasma membrane of tissue culture cells, resulting in both uptake and expression of the DNA. The technique is simple, highly reproducible, and effective for both transient and stable expression of transfected DNA. Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
Felgner
P L
PL
Institute of Bio-Organic Chemistry, Syntex Research, Palo Alto, CA 94303.
Gadek
T R
TR
Holm
M
M
Roman
R
R
Chan
H W
HW
Wenz
M
M
Northrop
J P
JP
Ringold
G M
GM
Danielsen
M
M
D000818:Animals; D002130:Calcium Phosphates; D002460:Cell Line; D003637:DEAE-Dextran; D005818:Genetic Engineering / Q000379:methods; D007202:Indicators and Reagents; D007739:L Cells / Q000166:cytology; D008081:Liposomes; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis*; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D002130:Calcium Phosphates; D007202:Indicators and Reagents; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; C054005:N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium; D003637:DEAE-Dextran