Platelet adhesion to subendothelial structures under flow conditions: no effect of the lipoxygenase product 13-HODE.
It has been shown that endothelial cells can convert linoleic acid to 13-hydroxyoctadecadienoic acid (13-HODE) and it has been suggested that 13-HODE has non-thrombogenic properties. However, no direct evidence has been presented that indicates that 13-HODE indeed modulates platelet-vessel wall interaction. In this study we have bound a purified 13-HODE to a thrombogenic surface and its effect on platelet adhesion was studied and compared to the effects of an analogous hydroxy fatty acid, 15-hydroxyeicosatetraenoic acid (15-HETE). The effect of 13-HODE on platelet adhesion was studied both under static and flow conditions. In this report we show that binding of up to 40 times the physiological concentration to a thrombogenic surface has no inhibitory effect on platelet adhesion under static or flow conditions. We conclude that 13-HODE is not an important regulatory substance in platelet-subendothelium interaction, although this does not exclude it has a putative anti-adhesive role on intact endothelium.
de Graaf
J C
JC
Dept. of Hematology, University Hospital Utrecht, The Netherlands.
Bult
H
H
de Meyer
G R
GR
Sixma
J J
JJ
de Groot
P G
PG
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology; D008041:Linoleic Acids / Q000378:metabolism / Q000494:pharmacology*; D010477:Perfusion; D010973:Platelet Adhesiveness / Q000187:drug effects*
D006893:Hydroxyeicosatetraenoic Acids; D008041:Linoleic Acids; C024348:13-hydroxy-9,11-octadecadienoic acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid