Metabolic effects of 3'-deoxyadenosine (cordycepin) and 2-halo-3'-deoxyadenosine on repair of X-ray-induced potentially lethal damage in Chinese hamster V79 cells.
Using cultured Chinese hamster V79 cells, an attempt has been made to examine the phosphorylation of cordycepin and its 2-halo derivatives (2-chloro-3'-deoxyadenosine, 2-bromo-3'-deoxyadenosine, and 2-iodo-3'-deoxyadenosine) by adenosine kinase, within the cells, and to correlate it with their cytotoxicities and abilities to inhibit the repair of X-ray-induced potentially lethal damage (PLDR). Of all compounds, only cordycepin was found to be phosphorylated and showed both potent cytotoxicity and ability to inhibit PLDR.
Hiraoka
W
W
Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Tanabe
K
K
Kuwabara
M
M
Sato
F
F
Matsuda
A
A
Ueda
T
T
D015762:2-Chloroadenosine / Q000031:analogs & derivatives*; D000248:Adenosine Kinase / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D003839:Deoxyadenosines / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D010770:Phosphotransferases / Q000378:metabolism*
D003839:Deoxyadenosines; C055992:2-bromo-3'-deoxyadenosine; C055993:2-iodo-3'-deoxyadenosine; D015762:2-Chloroadenosine; D004247:DNA; D010770:Phosphotransferases; D000248:Adenosine Kinase; C055994:2-chloro-3'-deoxyadenosine