Characterization of a human rotavirus strain which is possibly a naturally-occurring reassortant virus.
We investigated the antigenic and genetic characters of one of two human rotavirus strains 69M and 57M isolated in Indonesia, both of which showed a "super-short" RNA electrophoretic pattern (A. Hasegawa et al., Microbiol. Immunol. 28, 719-722, 1984). By an enzyme-linked immunosorbent assay with subgroup-specific monoclonal antibodies, one virus, strain 57M, was found to have subgroup II antigenicity. The cross-reaction of this strain, in a plaque neutralization test, with a serotype 4 strain was high whereas that of strain 69M was low. When radiolabeled RNA probes prepared from this virus were hybridized with RNAs from reference strains of different serotypes, treated with S-1 nuclease and then subjected to polyacrylamide gel electrophoresis, we found that (i) RNA segment 10 (the super-short segment) hybridized with that of another super-short pattern virus, strain 69M; (ii) segment 7, coding for a neutralization antigen, hybridized with that of the serotype 4 virus; (iii) segment 6, coding for a major inner-shell protein, hybridized with that of a serotype 1 virus; and (iv), some other segments hybridized with those of the reference viruses of serotypes other than 2 and 3. We suspect that this strain is possibly a naturally-occurring reassortant virus whose genetic segments are derived from different human rotaviruses.
Matsuno
S
S
Central Virus Diagnostic Laboratory, National Institute of Health, Tokyo, Japan.
Mukoyama
A
A
Hasegawa
A
A
Taniguchi
K
K
Inouye
S
S
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D001345:Autoradiography; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D012330:RNA, Double-Stranded; D012367:RNA, Viral