Dexamethasone-induced reduction of phospholipase D activity in the rat. Possible role of lipocortin.
Subcutaneous injection of dexamethasone resulted in a reduction of demonstrable phospholipase D activity of rat brain and liver microsomes. Partially purified rat lung lipocortin inhibited the activity of both microsomal and partially purified rat brain phospholipase D. These results show that phospholipase D activity is suppressed by dexamethasone and one of the possible mechanisms of inhibition may be a phospholipase inhibitory protein, lipocortin.
Kobayashi
M
M
Department of Biochemistry, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Bansal
V S
VS
Singh
I
I
Kanfer
J N
JN
D000315:Adrenalectomy; D000818:Animals; D017302:Annexins; D001921:Brain / Q000201:enzymology; D003907:Dexamethasone / Q000494:pharmacology*; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D006023:Glycoproteins / Q000502:physiology*; D008099:Liver / Q000201:enzymology; D008297:Male; D008780:Methyltransferases / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D050918:Phosphatidylethanolamine N-Methyltransferase; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D017302:Annexins; D006023:Glycoproteins; D003907:Dexamethasone; D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase; D002798:Diacylglycerol Cholinephosphotransferase; D010740:Phospholipases; D010739:Phospholipase D