Establishment and characterization of T-cell lines (Yana-I and Yana-II) derived from a patient with chronic adult T-cell leukemia (ATL).
ATL-cell lines producing C-type retroviruses were established by culture of the peripheral blood mononuclear cells from a patient with chronic ATL. These cell lines had the surface feature of inducer/helper T-cells with electron microscopic finding of C-type retrovirus on the cell surface. Chromosome analysis revealed the presence of a marker chromosome, but did not identify 7 trisomy nor 14q+. Of the cell lines, the line established by culture with TCGF was named "Yana-I" and the line established by culture of Yana-I without adding TCGF was named "Yana-II". Serum ATLA-antibody was positive in 1.5% of the healty individuals examined (4/257). Among malignant lymphoma patients, ATLA antibody was positive in none of the patients with B-cell type lymphoma (0/10) and Hodgkin's disease (0/2). In contrast, ATLA antibody was found in half of the patients with T-cell type lymphoma (3/6) and all with ATL (4/4). This suggested that C-type retrovirus in closely associated with ATLL.
Yamaguchi
A
A
Miura
I
I
Miura
A B
AB
Amano
Y
Y
D000914:Antibodies, Viral / Q000032:analysis; D002460:Cell Line; D017977:Deltaretrovirus / Q000276:immunology; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000382:microbiology; D008875:Middle Aged; D012190:Retroviridae; D013601:T-Lymphocytes / Q000382:microbiology
D000914:Antibodies, Viral; D005819:Genetic Markers