Phagocytotic activities of latex beads by FRTL cell: possible interaction of cell surface glycoprotein.
We investigated the phagocytic activity of cultured rat thyroid cells (FRTL) by using latex beads. FRTL cells ingested many carboxylate-modified polystyrene latex beads (c-latex beads), which had carboxyl residues on the surface, whereas they ingested few polystyrene latex beads (p-latex beads), which had no electrostatic charge. Phagocytotic activity of c-latex beads was stimulated by TSH in a dose-related manner. Dibutyryl, 8-bromo-cAMP, and forskolin also stimulated this process. Tunicamycin significantly inhibited dibutyryl cAMP-stimulated phagocytotic activity of c-latex beads by FRTL cells. N-acetylglucosaminidase also inhibited dibutyryl cAMP-stimulated phagocytosis. These results suggest that the interaction between negative surface charge of c-latex beads and positive surface charge probably induced by N-acetylglucosamine in the cell surface glycoprotein, might play an important role in the phagocytosis of FRTL cells.
Takazawa
K
K
Third Department of Internal Medicine, University of Yamanashi Medical School, Tamaho, Japan.
Endo
T
T
Onaya
T
T
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000118:Acetylglucosaminidase / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis; D006023:Glycoproteins / Q000378:metabolism*; D007840:Latex; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D013972:Thyrotropin / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology
D006023:Glycoproteins; D007840:Latex; D014415:Tunicamycin; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D003994:Bucladesine; D013972:Thyrotropin; D000242:Cyclic AMP; D000118:Acetylglucosaminidase