title: Enhancement of a secondary antibody response to vesicular stomatitis virus "G" protein by IFN-gamma treatment at primary immunization.
abstract: IFN-gamma is a secreted polypeptide product of stimulated T lymphocytes with immunomodulatory properties as well as antiviral activity. We have investigated the effects of IFN-gamma treatment on a neutralizing antibody response to vesicular stomatitis virus (VSV) when administered in conjunction with immunization using purified envelope glycoprotein "G" of VSV. Administration of rIFN-gamma to mice or cattle at the time of primary immunization with VSV G glycoprotein enhanced the magnitude of a secondary virus-neutralizing antibody response after a booster administration of the same Ag without IFN-gamma treatment. Enhancement was statistically significant and occurred at relatively low doses of IFN-gamma in the absence of any additional adjuvants. Furthermore, cattle treated with IFN-gamma at the time of a single primary immunization were more resistant to VSV challenge than those immunized without IFN-gamma treatment. IFN-gamma treatment in conjunction with a single primary immunization may therefore provide a practical means of enhancing protection from a viral challenge without the use of inflammatory adjuvants or booster immunizations.
authors.affiliation: Department of Pharmacological Sciences, Genentech, Inc., South San Francisco, CA 94080.
authors.forename: K P
authors.initials: KP
authors.lastname: Anderson
authors.forename: E H
authors.initials: EH
authors.lastname: Fennie
authors.forename: T
authors.initials: T
authors.lastname: Yilma
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D014645:Herpesvirus 3, Human / Q000276:immunology*; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000008:administration & dosage*; D008562:Membrane Glycoproteins; D051379:Mice; D008811:Mice, Inbred DBA; D009500:Neutralization Tests; D013280:Stomatitis / Q000209:etiology / Q000276:immunology* / Q000382:microbiology; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins / Q000008:administration & dosage* / Q000276:immunology; D014777:Virus Diseases / Q000209:etiology / Q000276:immunology* / Q000382:microbiology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D007371:Interferon-gamma