title: The Drosophila ets-2 gene: molecular structure, chromosomal localization, and developmental expression.
abstract: The cellular homologs of the ets gene from the avian erythroblastosis retrovirus E26 have been studied in chickens, humans, mice, and cats. In this report a further evolutionary step is taken by isolating and characterizing a Drosophila ets-related genomic clone. Sequence analysis of this clone has shown it to contain the 3' end of the v-ets gene, called ets-2, corresponding to the last two exons of chicken ets. The predicted amino acid sequence was found to have over 90% homology when compared to that of v-ets. This is the highest level of conservation observed for any previously characterized Drosophila oncogene homolog. Expression of the ets-2 gene occurs throughout development, but is highest during the embryonic and pupal stages. By in situ hybridization, the ets-2 chromosomal position was determined to be 58A/B which corresponds to no known phenotypic mutant. As this is a highly conserved gene, the Drosophila model system should prove useful for the determination of the ets gene function.
authors.affiliation: Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701-1013.
authors.forename: L J
authors.initials: LJ
authors.lastname: Pribyl
authors.forename: D K
authors.initials: DK
authors.lastname: Watson
authors.forename: M J
authors.initials: MJ
authors.lastname: McWilliams
authors.forename: R
authors.initials: R
authors.lastname: Ascione
authors.forename: T S
authors.initials: TS
authors.lastname: Papas
mesh_terms: D001355:Alpharetrovirus / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D005091:Exons; D005786:Gene Expression Regulation; D007438:Introns; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D011679:Pupa / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes