The 5'-CA DNA-sequence preference of daunomycin.
The DNA-sequence specificity of daunomycin was investigated by DNase I footprinting and an E. coli RNA polymerase transcription-inhibition assay. The 5'-CA sequence was identified as being the highest affinity binding site, although other modest affinity (5'-GC, CG, CT, TC, AC) and poor affinity sites (5'-AA, AT, TA) were also observed. The preference of daunomycin for 5'-CA nucleotide sequence suggests that its biological activity may arise from association with the 5'-CA-containing sequences thought to be associated with genetic regulatory elements in eukaryotes.
Skorobogaty
A
A
Department of Chemistry, La Trobe University, Bundoora, Victoria, Australia.
White
R J
RJ
Phillips
D R
DR
Reiss
J A
JA
D000225:Adenine; D001483:Base Sequence; D003596:Cytosine; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003630:Daunorubicin / Q000494:pharmacology; D003850:Deoxyribonuclease I; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D010957:Plasmids; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic / Q000187:drug effects
D003596:Cytosine; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D000225:Adenine; D003630:Daunorubicin