title: A comparison of the local environment of Cu(II) in native and half-Cu-depleted bovine serum amine oxidase.
abstract: Electron spin-echo envelope modulation spectroscopy has been used to compare the local environment of Cu(II) in native bovine serum amine oxidase, containing two copper atoms/dimer molecule, and in a protein preparation, half depleted of copper, which has little enzymatic activity. For each preparation, two different populations of coordinated imidazoles with inequivalent magnetic coupling to copper could be recognized. In addition, water was shown to be a ligand to copper. No differences in coordinated ligand structures between the native and half-Cu-depleted preparations could be seen. In addition, the amount of ambient, non-coordinated water detected for native and half-Cu-depleted proteins was found to be nearly equivalent. However, the addition of phenylhydrazine, an inhibitor that binds to the pyrroloquinoline quinone cofactor but not to Cu(II) in the native enzyme, displaces ambient water near copper.
authors.affiliation: Department of Biochemical Sciences, University La Sapienza, Rome.
authors.forename: B
authors.initials: B
authors.lastname: Mondovi
authors.forename: L
authors.initials: L
authors.lastname: Morpurgo
authors.forename: E
authors.initials: E
authors.lastname: Agostinelli
authors.forename: O
authors.initials: O
authors.lastname: Befani
authors.forename: J
authors.initials: J
authors.lastname: McCracken
authors.forename: J
authors.initials: J
authors.lastname: Peisach
mesh_terms: D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000032:analysis*; D001834:Body Water / Q000378:metabolism; D002417:Cattle; D003300:Copper / Q000097:blood*; D004578:Electron Spin Resonance Spectroscopy; D008667:Metalloproteins / Q000097:blood; D010084:Oxidation-Reduction; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000097:blood*; D010659:Phenylhydrazines / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D001798:Blood Proteins; D008667:Metalloproteins; D010659:Phenylhydrazines; C030299:phenylhydrazine; D003300:Copper; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors