title: Variation in the ability of Pseudomonas sp. strain B13 cultures to utilize meta-chlorobenzoate is associated with tandem amplification and deamplification of DNA.
abstract: Single-colony isolates of Pseudomonas sp. strain B13 were examined for their ability to utilize benzoate (Ben) and meta-chlorobenzoate (3CB) as the sole carbon source. Scoring of B13 cultures by the replica-plating technique revealed that under nonselective conditions, B13 spontaneously formed four different types of colonies: 3CB+ Ben+, 3CB+ Ben-, 3CB- Ben-, 3CB- Ben+. Successive testing of each of the four colony types showed that each produced the same four different types of single-colony isolates. Colonies of each class had characteristic phenotypic properties with respect to the accumulation of Ben or 3CB pathway intermediate products. The physical abundance of a 4.3-kilobase DNA encoding the first three enzymes of the chlorocatechol pathway correlated with the 3CB+ phenotype. Increased abundance of the 4.3-kilobase DNA fragment was the result of tandem amplification.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
authors.forename: V M
authors.initials: VM
authors.lastname: Rangnekar
mesh_terms: D001565:Benzoates / Q000378:metabolism; D019817:Benzoic Acid; D002723:Chlorobenzoates / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D005784:Gene Amplification; D005798:Genes, Bacterial; D014644:Genetic Variation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001565:Benzoates; D002723:Chlorobenzoates; D004269:DNA, Bacterial; C036427:3-chlorobenzoic acid; D019817:Benzoic Acid; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C018492:GCCNNNNNGGC-specific type II deoxyribonucleases