title: Convenient vectors for cloning and sequencing EcoRI and HindIII fragments.
abstract: The polylinker regions of plasmid pUC and bacteriophage M13mp vectors have been specifically modified to provide alternative positions for cloning and reexcising EcoRI and HindIII fragments; the EcoRI and HindIII sites have been moved internal to BamHI and Bg/II sites. The location of EcoRI and HindIII sites in these HinEco vectors allows either selective linearization or excision of the cloned fragments at unique flanking sites.
authors.affiliation: Hormone Research Institute, University of California, San Francisco 94143-0534.
authors.forename: H J
authors.initials: HJ
authors.lastname: Edenberg
authors.forename: L G
authors.initials: LG
authors.lastname: Moss
authors.forename: W J
authors.initials: WJ
authors.lastname: Rutter
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D009841:Oligonucleotides; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D001616:beta-Galactosidase