Heterogeneity in an isolated membrane protein. Has the 'authentic cytochrome oxidase' been identified?
The criteria of homogeneity or native state of a protein are prone to become ambiguous when applied to membrane proteins, such as cytochrome-c oxidase, which are purified by extraction with detergents. Properties of the purified material depend on the detergent used and on details of the purification protocol followed with any single batch of a preparation. We present arguments to show that the evidence presently available in published form does not justify the designation [(1987) J. Biol. Chem. 262, 3160-3164] of one type of preparation as being closer to the native state than others.
Hartzell
C R
CR
Department of Research, Alfred I. DuPont Institute, Wilmington, DE 19899.
Beinert
H
H
Babcock
G T
GT
Chan
S I
SI
Palmer
G
G
Scott
R A
RA
D002462:Cell Membrane / Q000201:enzymology*; D003576:Electron Transport Complex IV / Q000302:isolation & purification* / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism
D008565:Membrane Proteins; D003576:Electron Transport Complex IV