Lipoxygenase products in experimental septic arthritis in dogs.
Induction of an experimental septic arthritis was performed in the juvenile dog knee by an intra-articular injection with Staphylococcus aureus. From plasma, lipoxygenase (LO) products of arachidonic acid were separated by reversed-phase high-performance liquid chromatography (RP-HPLC) after extraction of lipids. The plasma samples did not contain UV-detectable amounts of LO products other than the LTB4 metabolite 20-COOH-LTB4, and 12-HETE. Small amounts of chemokinetically active material were found coeluting with the eluate fraction of 20-COOH-LTB4, LTB4 and 12-HETE after RP-HPLC. After 48 hours of septic arthritis, resulting in a massive acute joint inflammation, no significant changes in LO products were observed.
Herlin
T
T
Department of Pediatrics, Aarhus Kommunehospital, Denmark.
Fogh
K
K
Knudsen
V E
VE
Hansen
E S
ES
Bunger
E
E
Ewald
H
H
Holm
I
I
Kragballe
K
K
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood; D001170:Arthritis, Infectious / Q000201:enzymology* / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006614:Hindlimb; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D007975:Leukotriene B4 / Q000097:blood* / Q000502:physiology; D008055:Lipids / Q000302:isolation & purification; D008084:Lipoxygenase / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology; D013203:Staphylococcal Infections / Q000201:enzymology* / Q000276:immunology
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D008055:Lipids; D007975:Leukotriene B4; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008084:Lipoxygenase