Precipitation of solubilized Na+/K+-ATPase by divalent cations.
A method for preparation of membranous fragments of pure and highly active shark rectal gland Na+/K+-ATPase by Mn2+ precipitation of C12E8-solubilized enzyme is described. The method is rapid and inexpensive, and yields enzyme with a specific Na+/K+-ATPase activity of up to 1800 mumol/mg per h at 37 degrees C. The influence of the detergent/protein and lipid/protein ratios on the yield of membrane bound enzyme is described.
Esmann
M
M
Institute of Biophysics, University of Aarhus, Denmark.
D000818:Animals; D002413:Cations, Divalent; D002462:Cell Membrane / Q000201:enzymology; D004284:Dogfish; D007700:Kinetics; D012491:Salt Gland / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility
D002413:Cations, Divalent; D000254:Sodium-Potassium-Exchanging ATPase