Evidence for a receptor-mediated endocytosis of Alu I in Chinese hamster ovary cells.
Pretreatment of Chinese hamster ovary cells with proteases or with NaN3 leads to less chromosomal aberrations when the cells are posttreated with Alu I compared to the treatment of cells with Alu I alone. The same result is obtained when the cells are treated with Alu I at 0 degree C instead of 37 degrees C. The cells recover from the protease treatment when they are kept in medium before treatment with Alu I. These results are interpreted to mean that Alu I is bound by surface receptors and that the Alu I-receptor complexes are internalized by an energy-dependent endocytotic process.
Vasudev
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Institut für Genetik, Freie Universität Berlin, F.R.G.
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D000818:Animals; D001386:Azides / Q000633:toxicity; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D004705:Endocytosis; D005260:Female; D010053:Ovary; D010447:Peptide Hydrolases / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D019810:Sodium Azide
D001386:Azides; D011956:Receptors, Cell Surface; D019810:Sodium Azide; D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; D010447:Peptide Hydrolases