title: Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus subtilis.
abstract: We measured in Bacillus subtilis the recombination frequency between directly repeated 9 bp sequences flanking the transposon Tn5. Four different repeats were analyzed. They recombined with frequencies between 10(-7) and 10(-6) when they were carried on plasmids, and 150-1500 times less frequently when they were carried on the chromosome. We propose that this difference is due to the generation of single stranded DNA during plasmid replication.
authors.affiliation: Institut Jacques Monod, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Jannière
authors.forename: S D
authors.initials: SD
authors.lastname: Ehrlich
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004261:DNA Replication; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded