SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene.
Monkey cells synthesizing SV40 large T antigen were lysed and the extracts immunoprecipitated with either monoclonal anti-T antibody or monoclonal antibody to p110-114, the product of the retinoblastoma susceptibility gene (Rb). T and p110-114 coprecipitated in each case, implying that the proteins are complexed with each other. Substitution and internal deletion mutants of T that contain structural alterations in a ten residue, transformation-controlling domain failed to complex with p110-114. In contrast, T mutants bearing structural changes outside of this domain bound to p110-114. These results are consistent with a model for transformation by SV40 which, at least in part, involves T/p110-114 complex formation and the perturbation of Rb protein and/or T function.
DeCaprio
J A
JA
Division of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute, Boston, Massachusetts.
Ludlow
J W
JW
Figge
J
J
Shew
J Y
JY
Huang
C M
CM
Lee
W H
WH
Marsilio
E
E
Paucha
E
E
Livingston
D M
DM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D004198:Disease Susceptibility; D004591:Electrophoresis, Polyacrylamide Gel; D005134:Eye Neoplasms / Q000235:genetics*; D000882:Haplorhini; D007118:Immunoassay; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012175:Retinoblastoma / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming; D009363:Neoplasm Proteins; D010750:Phosphoproteins