Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus.
A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.
Chisaka
O
O
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Araki
K
K
Ochiya
T
T
Tsurimoto
T
T
Hiranyawasitte-Attatippaholkun
W
W
Yanaihara
N
N
Matsubara
K
K
D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000382:microbiology; D002460:Cell Line; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms; D010957:Plasmids; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
D014764:Viral Proteins