title: Effects of pertussis toxin on growth factor-stimulated inositol phosphate formation and DNA synthesis in Swiss 3T3 cells.
abstract: We have compared the effects of pretreatment of Swiss 3T3 cell with pertussis toxin on the stimulation of DNA synthesis and phosphoinositide hydrolysis in response to a wide variety of mitogens. The toxin substantially inhibited the stimulation of DNA synthesis in response to a phorbol ester or various peptide and polypeptide growth factors irrespective of their ability to activate phosphoinositidase C. Production of inositol phosphates in response to platelet-derived growth factor, fibroblast growth factor and prostaglandin F2 alpha were unaffected by the toxin while bombesin- and vasopressin-stimulated formation of inositol phosphates were inhibited by only 27 and 23% respectively. These results argue against a major role for a pertussis toxin-sensitive G protein in coupling any of these mitogen receptors to activation of a phosphoinositidase C. Furthermore, the results suggest that the widespread inhibitory effects of pertussis toxin on mitogen-stimulated DNA synthesis may be unrelated to the toxin's limited actions on phosphoinositide hydrolysis.
authors.affiliation: AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, Cambridge, U.K.
authors.forename: C W
authors.initials: CW
authors.lastname: Taylor
authors.forename: D M
authors.initials: DM
authors.lastname: Blakeley
authors.forename: A N
authors.initials: AN
authors.lastname: Corps
authors.forename: M J
authors.initials: MJ
authors.lastname: Berridge
authors.forename: K D
authors.initials: KD
authors.lastname: Brown
mesh_terms: D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis*; D006133:Growth Substances / Q000494:pharmacology*; D007295:Inositol Phosphates / Q000378:metabolism*; D051379:Mice; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D006133:Growth Substances; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D013403:Sugar Phosphates; D010566:Virulence Factors, Bordetella; D004247:DNA; D037342:Pertussis Toxin; D001839:Bombesin; D013936:Thymidine