Analysis of the TNF receptor on KYM cells by binding assay and affinity cross-linking.
The existence of a tumor necrosis factor (TNF) receptor on KYM human myosarcoma cells was elucidated by Scatchard plot and cross-linking analysis. The average number of specific binding sites per cell was 15,300 and the apparent dissociation constant Kd was 4.0 X 10(-10) M. The estimated relative molecular mass of a TNF receptor on KYM cell was approximately 95,000.
Niitsu
Y
Y
Department of Internal Medicine, Sapporo Medical College, Japan.
Watanabe
N
N
Sone
H
H
Neda
H
H
Yamauchi
N
N
Maeda
M
M
Urushizaki
I
I
D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D009217:Myosarcoma / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis*; D018124:Receptors, Tumor Necrosis Factor; D012509:Sarcoma / Q000032:analysis*
D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor