Effects of increasing cyclic AMP or calcium on feline erythroid progenitors in vitro: normal cells are stimulated while cells from retrovirus-infected cats are suppressed.
Pharmacologic modulators of cyclic 3',5'-adenosine monophosphate (cAMP) and calcium were added to cultures of bone marrow cells from normal cats and cats with retrovirus-induced erythroid aplasia (EA). Treatment with the following reagents increased the number of erythroid progenitors (CFU-e and BFU-e) in cells from normal cats: isoproterenol, dibutyryl cAMP, forskolin, RO-20-1724 and A23187. However, treatment of cells collected from viremic cats not only failed to enhance CFU-e and BFU-e but inhibited their growth. These studies suggest that EA is related to a non-reversible block of primitive erythroid progenitors or to direct inhibition of BFU-e and CFU-e growth.
Zack
P M
PM
Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
Kociba
G J
GJ
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002371:Cat Diseases / Q000503:physiopathology; D002415:Cats; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000503:physiopathology; D004920:Erythropoiesis / Q000187:drug effects; D005256:Leukemia Virus, Feline / Q000472:pathogenicity; D012010:Red-Cell Aplasia, Pure / Q000209:etiology / Q000662:veterinary; D012192:Retroviridae Infections / Q000150:complications / Q000503:physiopathology* / Q000662:veterinary; D012191:Retroviridae Proteins / Q000494:pharmacology; D015682:Retroviridae Proteins, Oncogenic; D013234:Stem Cells / Q000187:drug effects* / Q000503:physiopathology
D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; C051525:feline leukemia virus protein p15E; D000242:Cyclic AMP; D002118:Calcium