title: Bisphosphoglycerate mutase and pyruvate kinase activities during maturation of reticulocytes and ageing of erythrocytes.
abstract: An increase in bisphosphoglycerate mutase (BPGM) and a decrease in pyruvate kinase (PK), i.e. a decrease in PK/BPGM ratio, was observed in red cell populations from anemic rats containing 95% down to 3% reticulocytes in blood. Such a ratio has been used to study the fractionation of recticulocytes, according to their degree of maturation, after counter-current distribution of those cell populations in dextranpoly (ethylene glycol) two-phase systems. When applying this procedure to the fractionation according to age of erythrocytes from normal rats, the decrease of PK with cellular age was observed without a significant variation in BPGM activity.
authors.affiliation: Departmento de Bioquimica y Biologia Molecular, Universidad de Alcalã de Henares, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Luque
authors.forename: M D
authors.initials: MD
authors.lastname: Delgado
authors.forename: P
authors.initials: P
authors.lastname: Rodriguez-Horche
authors.forename: M T
authors.initials: MT
authors.lastname: Company
authors.forename: M
authors.initials: M
authors.lastname: Pinilla
mesh_terms: D000740:Anemia / Q000201:enzymology; D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000097:blood*; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000201:enzymology*; D004920:Erythropoiesis; D010770:Phosphotransferases / Q000097:blood*; D011770:Pyruvate Kinase / Q000097:blood*; D051381:Rats; D012156:Reticulocytes / Q000201:enzymology*
chemical_list: D010770:Phosphotransferases; D011770:Pyruvate Kinase; D001731:Bisphosphoglycerate Mutase