title: Effect of adrenocorticotropin and dietary ascorbic acid on cutaneous basophil hypersensitivity to phytohemagglutinin in chickens.
abstract: Two trials were conducted to assess the effects of adrenocorticotropin (ACTH) and dietary ascorbic acid (AA) on cutaneous basophil hypersensitivity (CBH) to phytohemagglutinin (PHA-P) in chickens. Broiler chicks received AA at levels of 0, 150, or 300 mg/kg of feed (ppm) continuous from hatching. At 6 to 7 wk of age, birds from each AA group received either 2 IU ACTH/100 g of body weight, 4% gelatin, or no ACTH or gelatin injections. Injections were given 12 h prior to, at the time of, and at 12 and 24 h after an intradermal wattle injection with PHA-P. Responses to PHA-P were determined as wattle indices. Injections of ACTH reduced body weight gain in both trials and decreased relative bursa weight in Trial 1. Injections of ACTH and dietary AA increased plasma cholesterol in both trials. Peak CBH wattle response occurred at 24 h post PHA-P injection. Injections of ACTH decreased mean wattle index at 18 and 36 h post PHA-P injection in Trial 1 and 18 and 24 h post PHA-P injection in Trial 2. The addition of AA ameliorated the ACTH-mediated suppression of CBH in a dose-related manner.
authors.affiliation: Department of Poultry Science, North Carolina State University, Raleigh 27695-7608.
authors.forename: D L
authors.initials: DL
authors.lastname: Murray
authors.forename: J
authors.initials: J
authors.lastname: Brake
authors.forename: J P
authors.initials: JP
authors.lastname: Thaxton
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002645:Chickens / Q000276:immunology*; D002784:Cholesterol / Q000097:blood; D003129:Comb and Wattles / Q000187:drug effects; D006968:Hypersensitivity, Delayed / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D007111:Immunity, Cellular / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D009929:Organ Size; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012867:Skin / Q000166:cytology
chemical_list: D010835:Phytohemagglutinins; D000324:Adrenocorticotropic Hormone; D002784:Cholesterol; D001205:Ascorbic Acid