title: New rapid assay for factor XIa-alpha 1 antitrypsin complex--application to DIC.
abstract: We developed a new rapid assay for the factor XIa-alpha 1 antitrypsin in complex (F.XIa-alpha 1 AT) in plasma with the use of a newly produced anti-F.XI monoclonal antibody (KMXI-1). This assay was completed within about 5 hours, and the minimum assay range extended sensitivity about 5-fold over the former assay (Thromb. Res. 44, 489-501, 1986). In 20-fold diluted plasma samples, this assay was not affected by co-existing F.XI or nonspecific color development of the plasma. Normal levels of F.XIa-alpha 1 AT (11 +/- 4.1 ng/ml plasma [n = 96]) increased with the aging of healthy adults. The F.XIa-alpha 1 AT levels of patients with disseminated intravascular coagulation (DIC) rose along with the progression of the disease, and the appearance of high levels and the peak of F.XIa-alpha 1 AT developed faster than FDP-E or alpha 2 plasmin inhibitor-plasmin-complex in most patients. These results indicate that, in addition to FDP, F.XIa-alpha 1 AT is a useful molecular marker for DIC.
authors.affiliation: Department of Clinico, Kansai Medical University, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Komiyama
authors.forename: M
authors.initials: M
authors.lastname: Masuda
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: H
authors.initials: H
authors.lastname: Egawa
authors.forename: H
authors.initials: H
authors.lastname: Nishikado
authors.forename: K
authors.initials: K
authors.lastname: Murata
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000097:blood*; D004211:Disseminated Intravascular Coagulation / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D015945:Factor XIa / Q000032:analysis*; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D000515:alpha 1-Antitrypsin / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D005338:Fibrin Fibrinogen Degradation Products; D000515:alpha 1-Antitrypsin; D015945:Factor XIa