An investigation of glucose uptake in relation to steroidogenesis in rat testis and tumour Leydig cells.
The mechanisms of the requirement of glucose for steroidogenesis were investigated by monitoring the uptake of the glucose analogue 2-deoxy-D-glucose by rat testis and tumour Leydig cells. The characteristics of glucose transport in both of these cell types were found to resemble those of the facilitated-diffusion systems for glucose found in most other mammalian cells. The Leydig cells took up 2-deoxy-D-glucose but not L-glucose, and the uptake was inhibited by both cytochalasin B and forskolin. In the presence of luteinizing hormone, the rate of 2-deoxy-D-glucose uptake by both cell types was increased by approx. 50%. In addition to D-glucose, it was shown that the Leydig cells could also utilize 3-hydroxybutyrate or glutamine to maintain steroidogenesis.
Amrolia
P
P
Department of Biochemistry, Royal Free Hospital School of Medicine (University of London), U.K.
Sullivan
M H
MH
Garside
D
D
Baldwin
S A
SA
Cooke
B A
BA
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003837:Deoxy Sugars / Q000493:pharmacokinetics*; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D051381:Rats; D013736:Testicular Neoplasms / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis*
D003837:Deoxy Sugars; D005576:Colforsin; D003571:Cytochalasin B; D013739:Testosterone; D003994:Bucladesine; D007986:Luteinizing Hormone; D003847:Deoxyglucose