Modulating effects by Trichinella spiralis sensu stricto excretory/secretory antigens of human neutrophil functions.
Many parasites are able to modify the host response through different mechanisms, for example host leukocyte function modification. Since granulocytes represent the most important effector cells against Trichinella spiralis, we evaluated the effects of supernatants of T. spiralis sensu stricto muscle larvae cultured for at least 18 hours (T. Sup.) on some human neutrophil functions, i.e. chemotaxis, phagocytosis and Nitroblue tetrazolium dye reduction. The cells from blood donors were tested in the presence of T. Sup. at different concentrations (1, 5, 10, 20%). The results show no effect of T. Sup. on neutrophil phagocytosis. On the contrary, random migration of cells is increased at concentrations of 10-20%, while stimulated migration is significantly decreased at concentrations of 5-10% but not at 20%. We found a significant decrease of Nitroblue tetrazolium reduction at 5-10% concentrations of T. Sup. It is concluded that T. spiralis culture supernatants contain some factors capable of modifying human neutrophil chemotaxis, oxidative metabolism, but not phagocytosis.
Bruschi
F
F
Carulli
G
G
Azzara
A
A
Marini
A
A
Ruocco
L
L
Ambrogi
F
F
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D009580:Nitroblue Tetrazolium; D010587:Phagocytosis / Q000502:physiology; D051381:Rats; D014234:Trichinella / Q000276:immunology*
D000947:Antigens, Helminth; D009580:Nitroblue Tetrazolium