title: Production of hepatitis B virus surface antigen particles by the human hepatoma cell line huGK-14 in a serum-free medium.
abstract: The human hepatoma cell line, huGK-14, containing integrated hepatitis B virus (HBV) DNAs, produced a large number of 23 nm spherical particles of HBV surface antigen (HBsAg) in a serum-free medium. These particles closely resembled the HBsAg particles found in the sera of HBV carriers and elicited a high antibody response in guinea pigs. The huGK-14 cells in a serum-free medium would provide a new, economic source of HBV vaccine.
authors.affiliation: Department of Gene Research, Japanese Foundation for Cancer Research, Tokyo.
authors.forename: K
authors.initials: K
authors.lastname: Koike
authors.forename: E
authors.initials: E
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Katagiri
authors.forename: M
authors.initials: M
authors.lastname: Katayanagi
authors.forename: M
authors.initials: M
authors.lastname: Oda
authors.forename: H
authors.initials: H
authors.lastname: Tsunoo
authors.forename: K
authors.initials: K
authors.lastname: Yaginuma
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D000917:Antibody Formation; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002460:Cell Line; D002846:Chromatography, Affinity; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology*
chemical_list: D003470:Culture Media; D006514:Hepatitis B Surface Antigens