Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA.
The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31 cells even in serum-free medium. They were able to form colonies in soft agar. The phenotypic alteration in the transformed cells was reversed by the addition of anti-human bFGF antibodies to the medium. These results suggest that the cellular transformation mediated by bFGF is caused by autocrine stimulation with secreted bFGF molecules.
Sasada
R
R
Central Research Division, Takeda Chemical Industries, Ltd., Osaka, Japan.
Kurokawa
T
T
Iwane
M
M
Igarashi
K
K
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005346:Fibroblast Growth Factors / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D014162:Transfection
D005346:Fibroblast Growth Factors; D004247:DNA; D004262:DNA Restriction Enzymes