Construction of a yeast mutant lacking the mitochondrial nuclease.
The nuclear gene from Saccharomyces cerevisiae that encodes the major mitochondrial nuclease was cloned. Gene sequences were identified from a lambda gt11 library by antibodies specific to the mitochondrial nuclease. DNA from the phage recombinant was used to isolate the entire nuclease gene from a plasmid library. Yeast strains containing the nuclease gene on a multicopy plasmid vector overproduced mitochondrial nuclease 20-40 times relative to a wild-type strain. Strains containing a null allele of the nuclease gene lacked all traces of mitochondrial nuclease. Both cell types, however, were phenotypically wild-type indicating that the nuclease is not an essential enzyme for mitochondrial function. The locus encoding the mitochondrial nuclease is termed NUC1.
Zassenhaus
H P
HP
Department of Microbiology, St Louis University Medical Center, MO 63104.
Hofmann
T J
TJ
Uthayashanker
R
R
Vincent
R D
RD
Zona
M
M
D004720:Endonucleases / Q000235:genetics*; D005092:Exonucleases / Q000235:genetics*; D005796:Genes; D005800:Genes, Fungal; D008928:Mitochondria / Q000201:enzymology*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D004720:Endonucleases; D005092:Exonucleases; C587067:Nuc1 protein, S cerevisiae