In situ localization of human papilloma virus type 16 DNA in a metastasis of an endocervical adenocarcinoma.
Metastatic tissue from an endocervical adenocarcinoma was examined for human papilloma virus (HPV) DNA sequences. Southern blot analysis with 32P-labeled viral probes revealed the presence of about 150 copies of HPV-16 DNA per cellular genome. DNA in situ hybridization of frozen sections with biotinylated probes showed that HPV-16 DNA was exclusively detectable in the nuclei of the malignant epithelial cells and not in the stroma.
Walboomers
J M
JM
Department of Pathology, University of Amsterdam, The Netherlands.
Fokke
H E
HE
Polak
M
M
Volkers
H
H
Houthoff
H J
HJ
Barents
J
J
van der Noordaa
J
J
ter Schegget
J
J
D000230:Adenocarcinoma / Q000382:microbiology* / Q000556:secondary; D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D010386:Pelvic Neoplasms / Q000382:microbiology* / Q000556:secondary; D014412:Tumor Virus Infections / Q000382:microbiology; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D004279:DNA, Viral