Two isoforms of protein phosphatase 1 may be produced from the same gene.
A cDNA clone encoding a second type-1 protein phosphatase catalytic subunit (1 alpha) was isolated from a rabbit skeletal muscle cDNA library constructed in lambda gt10. The deduced protein sequence (330 residues, 37.5 kDa) was 19 residues longer at its N-terminus than protein phosphatase 1 beta(311 residues, 35.4 kDa). The amino acid sequences of protein phosphatases 1 alpha and 1 beta were identical after residue 33 of protein phosphatase 1 alpha. The results suggest that the different N-terminal sequences of protein phosphatases 1 alpha and 1 beta are likely to be generated by differential transcription or processing of the mRNA transcribed from a single gene. Southern blotting of rabbit DNA was consistent with this interpretation.
Cohen
P T
PT
Department of Biochemistry, The University, Dundee, Scotland.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D009132:Muscles / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D054645:Protein Phosphatase 1; D011817:Rabbits
D007527:Isoenzymes; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1