Different metastatic aggressiveness by murine TS/A clones: ultrastructure, extracellular glycoproteins and type IV collagenolytic activity.
Cell ultrastructure, extracellular matrix glycoproteins, and type IV collagenolytic activity have been examined in four murine TS/A clones characterized by different metastatic aggressiveness. In vitro, highly metastatic clones (E) exhibited slightly less differentiated ultrastructure, and high type IV collagenolytic activity, while low metastatic clones (F) showed a more differentiated cytotype, with either high and low collagenolytic activity. Type IV collagen, laminin, and fibronectin were expressed without correlation with the metastatic efficiency; keratin was slightly more evident in E than in F cells. The morphological differences between E and F clones were less evident in the tumors produced by subcutaneous injection, and markedly reduced in the relative metastases composed only of the less differentiated cytotype; differences were also reduced in cells cultured on extracellular-matrix-coated flasks. The present study shows that no general correlation is observed between the studied parameters and metastatic aggressiveness.
Garbisa
S
S
Histology Institute, Medical School of Padova, Italy.
De Giovanni
C
C
Biagini
G
G
Vasi
V
V
Grigioni
W F
WF
D'Errico
A
A
Mancini
A M
AM
Del Re
B
B
Lollini
P L
PL
Nanni
P
P
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D002999:Clone Cells; D005109:Extracellular Matrix / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000096:biosynthesis*; D008325:Mammary Neoplasms, Experimental / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D003012:Microbial Collagenase / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D009362:Neoplasm Metastasis / Q000378:metabolism* / Q000648:ultrastructure; D010641:Phenotype; D014407:Tumor Cells, Cultured
D006023:Glycoproteins; D003012:Microbial Collagenase