[Molecular genetic study of the changes in the incompatibility and regulation of the transfer of the F-like pAP18-1 plasmid induced by nitrosoguanidine and the Tn5 and Tn9 transposons].
Relation between induced mutations of plasmid pAP18-1 (Tc, Col) and alterations in it's restriction map was studied. Nitrosoguanidine induced mutations of transfer regulation system and incompatibility of this plasmid related with alteration in the situation of recognition sites for restrictases EcoR1 and Sal1 in map positions 42.2-4.3 and 12.9-17.9 MD. Insertions of transposons Tn5 and Tn9 into the plasmid DNA resulted in a decrease of incompatibility level.
Shchipkov
V P
VP
Buianova
N I
NI
Shcherbakova
E V
EV
Pekhov
A P
AP
D001483:Base Sequence / Q000187:drug effects; D002874:Chromosome Mapping; D003227:Conjugation, Genetic / Q000187:drug effects; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005144:F Factor / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005819:Genetic Markers / Q000187:drug effects; D009154:Mutation; D009604:Nitrosoguanidines / Q000494:pharmacology*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005819:Genetic Markers; D009604:Nitrosoguanidines; D004262:DNA Restriction Enzymes
Molekuliarno-geneticheskoe izuchenie izmeneniĭ nesovmestimosti i reguliatsii perenosa F-podobnoĭ plazmidy pAP18-1, indutsirovannykh nitrozoguanidinom i transpozonami Tn5 i Tn9.