title: A 46 KD polypeptide, present in purified human cytomegalovirus, is provided with DNase activity and is antigenically related to a higher molecular weight, enzymatically inactive, cellular protein.
abstract: DNase activity can be detected in lysates of extensively purified human cytomegalovirus (HCMV) particles. The presence of activity only in purified virions and not in dense bodies and its neutralization by HCMV-positive human sera strongly suggest that the enzyme is virus-specific. In situ analysis of the enzymatic activity of individual HCMV polypeptides, separated in a DNA-containing polyacrylamide gel, has shown that the DNase activity present in purified virions is mainly associated with a polypeptide of 46,000 dalton MW (p46). A monospecific anti-p46 serum, raised in mice, neutralizes about 80% of the DNase activity present in extracts of purified virions and recognizes an enzymatically inactive, 76,000 dalton MW polypeptide both in non-infected and HCMV-infected cells.
authors.affiliation: Institute of Microbiology, University of Bologna, Medical Faculty, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ripalti
authors.forename: M P
authors.initials: MP
authors.lastname: Landini
authors.forename: M
authors.initials: M
authors.lastname: La Placa
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D003587:Cytomegalovirus / Q000032:analysis / Q000201:enzymology* / Q000276:immunology; D003851:Deoxyribonucleases / Q000276:immunology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D014771:Virion / Q000032:analysis / Q000201:enzymology
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins; D003851:Deoxyribonucleases