Expression of the Escherichia coli xylose isomerase gene in Saccharomyces cerevisiae.
Transformation of Saccharomyces cerevisiae by yeast expression plasmids bearing the Escherichia coli xylose isomerase gene leads to production of the protein. Western blotting (immunoblotting) experiments show that immunoreactive protein chains which comigrate with the E. coli enzyme are made in the transformant strains and that the amount produced parallels the copy number of the plasmid. When comparable amounts of immunologically cross-reactive xylose isomerase protein made in E. coli or S. cerevisiae were assayed for enzymatic activity, however, the yeast protein was at least 10(3)-fold less active.
Sarthy
A V
AV
Department of Genetics, University of Washington, Seattle 98195.
McConaughy
B L
BL
Lobo
Z
Z
Sundstrom
J A
JA
Furlong
C E
CE
Hall
B D
BD
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005800:Genes, Fungal; D007118:Immunoassay; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
D012333:RNA, Messenger; D004262:DNA Restriction Enzymes; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase