title: Intracellular pH regulation in the mouse lacrimal gland acinar cells.
abstract: Intracellular pH (pHi) of the acinar cells of the isolated, superfused mouse lacrimal gland has been measured using pH-sensitive microelectrodes. Under nonstimulated condition pHi was 7.25, which was about 0.5 unit higher than the equilibrium pH. Alterations of the external pH by +/- 0.4 unit shifted pHi only by +/- 0.08 unit. The intracellular buffering value determined by applications of 25 mM NH4+ and bicarbonate buffer solution gassed with 5% CO2/95% O2 was 26 and 46 mM/pH, respectively. Stimulation with 1 microM acetylcholine (ACh) caused a transient, small decrease and then a sustained increase in pHi. In the presence of amiloride (0.1 mM) or the absence of Na+, application of ACh caused a significant decrease in pHi and removal of amiloride or replacement with Na+-containing saline, respectively, rapidly increased the pHi. Pretreatment with DIDS (0.2 mM) did not change the pHi of the nonstimulated conditions; however, it significantly enhanced the increase in pHi induced by ACh. The present results showed that (i) there is an active acid extrusion mechanism that is stimulated by ACh; (ii) stimulation with ACh enhances the rate of acid production in the acinar cells; and (iii) the acid extrusion mechanism is inhibited by amiloride addition to and Na+ removal from the bath solution. We suggest that both Na+/H+ and HCO3-/Cl- exchange transport mechanisms are taking roles in the intracellular pH regulation in the lacrimal gland acinar cells.
authors.affiliation: Department of Physiology, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Ozawa
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: A
authors.initials: A
authors.lastname: Nishiyama
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D005110:Extracellular Space / Q000502:physiology; D006706:Homeostasis; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000502:physiology; D007700:Kinetics; D007765:Lacrimal Apparatus / Q000166:cytology / Q000502:physiology*; D051379:Mice; D017923:Sodium-Hydrogen Exchangers
chemical_list: D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D000109:Acetylcholine