title: Interaction between the delta-endotoxin produced by Bacillus thuringiensis ssp. entomocidus and liposomes.
abstract: The delta-endotoxin produced by Bacillus thuringiensis ssp. entomocidus induced the release of encapsulated [14C]sucrose from reverse-phase vesicles composed of phosphatidylcholine and cholesterol. No such release was detected when the phospholipid component of the vesicles was either phosphatidylethanolamine, phosphatidylglycerol, or sphingomyelin. The toxin-induced release was competitively inhibited by negatively charged organic ions while positively charged organic ions, apart from choline chloride, had no such effect. The existence of a polar head group in the phospholipid as well as intermolecular hydrogen bonding at the membrane surface, was found to be of major importance in the toxin-liposome interaction.
authors.affiliation: Institute for Nature Conservation Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Yunovitz
authors.forename: A
authors.initials: A
authors.lastname: Yawetz
mesh_terms: D000838:Anions; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002463:Cell Membrane Permeability; D002784:Cholesterol; D004563:Electrochemistry; D004731:Endotoxins / Q000494:pharmacology*; D006460:Hemolysin Proteins; D006860:Hydrogen Bonding; D008081:Liposomes / Q000378:metabolism*; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D013109:Sphingomyelins; D013395:Sucrose / Q000378:metabolism
chemical_list: D000838:Anions; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D013109:Sphingomyelins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D013395:Sucrose; D002784:Cholesterol