myb oncogene in human hematopoietic neoplasia with 6q- anomaly.
Molecular and cytogenetic analyses were performed on human T-cell leukemia cell lines (PEER and MOLT-4) with the 6q- anomaly. The PEER cells contained an interstitial deletion of the long arm of chromosome 6, that is, del(6)(q13q21), as well as other changes. The MOLT-4 cells showed a terminal deletion of the long arm of chromosome 6, that is, del(6)(q24). The 700-bp BamHI/XbaI-digested c-myb probe hybridized to a 4.3-kb fragment in EcoRI digested DNAs from these two cell lines, showing no deletion, rearrangement, or amplification. On the other hand, ML cells [ML-1, -2 and -3; human myeloid/T-cell biphenotypic leukemia cell lines with del(6)(q24)] showed an amplification of the c-myb gene and had a high level of the c-myb-related mRNA at 3.5 kb. Though no amplification of the c-myb at the DNA level was noted in the PEER or MOLT-4 cell lines, apparent high expression of the c-myb was detected in these human T-cell neoplastic lines. These results indicate that high c-myb expression is related to lineage of hematopoietic neoplasia rather than to the 6q- change.
Ohyashiki
K
K
Department of Internal Medicine, Tokyo Medical College, Japan.
Ohyashiki
J H
JH
Kinniburgh
A J
AJ
Toyama
K
K
Ito
H
H
Minowada
J
J
Sandberg
A A
AA
D002871:Chromosome Banding; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011519:Proto-Oncogenes; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured
D005819:Genetic Markers; D004262:DNA Restriction Enzymes