[Isolation of a stable cell line after transfection of rat embryo fibroblasts by the gene of the polyomavirus large T antigen].
A stable cell line REF(LT) was established upon transfection of DNA plasmid containing a large T gene of polyoma virus. REF(LT) cells grow in a monolayer, their growth depends on the underlayer, they are non-carcinogenic. The dependence of cellular growth on serum factors is decreased.
Komissarova
E V
EV
Revazova
E S
ES
Kiselev
F L
FL
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology*; D005814:Genes, Viral; D010957:Plasmids; D011120:Polyomavirus / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral
Poluchenie stabil'noĭ kletochnoĭ linii posle transfektsii krysinykh émbrional'nykh fibroblastov gena bol'shogo T-antigena virusa poliomy.