Phrenic nerve-diaphragm preparation in situ for studying the detoxification of soman in the rat liver.
Detoxification of soman (1,2,2'-trimethylpropyl methylphosphonofluoridate) in the liver was studied in the rat phrenic nerve-diaphragm preparation in situ. Contractions of diaphragm induced by electrical stimulation of the phrenic nerve were continuously recorded. Twitch height depression caused by soman was influenced by the route of application (portal or jugular vein) indicating its rate of detoxification in the liver. Pretreatment with phenobarbital reduced, whereas previous application of tri-O-cresyl phosphate (TOCP) enhanced, effectiveness of soman administered i.p. Possible mechanisms of detoxification and the features of the method are discussed.
Cetković
S
S
Medical Department, Military Technical Institute, Belgrade, Yugoslavia.
Bosković
B
B
D000818:Animals; D003964:Diaphragm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D008658:Inactivation, Metabolic; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000502:physiology; D010634:Phenobarbital / Q000494:pharmacology; D010791:Phrenic Nerve / Q000187:drug effects; D010968:Plasticizers / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012999:Soman / Q000493:pharmacokinetics / Q000633:toxicity*; D009435:Synaptic Transmission; D014317:Tritolyl Phosphates / Q000494:pharmacology
D010968:Plasticizers; D014317:Tritolyl Phosphates; D012999:Soman; C025541:tri-o-cresyl phosphate; D010634:Phenobarbital