The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c.
The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol1- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differentially regulated by the product of CYP1. The HAP1 gene codes for a product which has previously been proved to be necessary for the expression of the heme dependent CYC1-UAS1 cis regulatory sequence. In this article, we show by complementation and recombination that CYP1 and HAP1 are the same gene, moreover we identify hap1-1 as an iso2-cytochrome c underproducer mutation of the CYP1 gene.
Verdière
J
J
Centre de Génétique Moléculaire du C.N.R.S., Université Pierre et Marie Curie, Gif-sur-Yvette, France.
Creusot
F
F
Guarente
L
L
Slonimski
P P
PP
D000483:Alleles; D003574:Cytochrome c Group / Q000235:genetics*; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D005809:Genes, Regulator; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D003574:Cytochrome c Group