Arachidonic acid-induced pleurisy (ARIP): an in vivo model for testing 5-lipoxygenase inhibition.
Arachidonic acid-induced pleurisy in the rat was evaluated for testing inhibitors of arachidonic acid (AA) metabolism. The model involves the administration of AA intrapleurally and the determination of LTB4 and PGE2 as indicators of 5-lipoxygenase and cyclooxygenase activities in the exudate/wash. This model is suitable for the in vivo evaluation of potential inhibitors of the 5-lipoxygenase pathway but not the cyclooxygenase pathway of AA cascade.
Boctor
A M
AM
Department of Pharmacology, Warner-Lambert/Parke-Davis Pharmaceutical Research, Ann Arbor, Michigan.
Burke
C
C
Hovinga
M E
ME
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D000818:Animals; D001091:Arachidonate Lipoxygenases / Q000037:antagonists & inhibitors*; D001095:Arachidonic Acids / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors; D015232:Dinoprostone; D004195:Disease Models, Animal; D007213:Indomethacin / Q000494:pharmacology; D007975:Leukotriene B4 / Q000378:metabolism; D016859:Lipoxygenase Inhibitors; D008297:Male; D010998:Pleurisy / Q000139:chemically induced / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D001095:Arachidonic Acids; D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D011458:Prostaglandins E; D011720:Pyrazoles; D007975:Leukotriene B4; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D001091:Arachidonate Lipoxygenases; C015429:phenidone; D015232:Dinoprostone; D007213:Indomethacin