The reaction of nitric oxide with copper proteins and the photodissociation of copper-NO complexes.
The reactivity with nitric oxide was investigated for a number of type-1, type-2 and type-3 copper proteins azurin from Pseudomonas aeruginosa (type-1 copper); bovine superoxide dismutase, diamine oxidase from pig kidney and galactose oxidase from Dactylium dendroides (type-2 copper); haemocyanin from Helix pomatia (type-3 copper); the blue oxidases ceruloplasmin from pig serum, and ascorbate oxidase from Cucurbita pepo medullosa. Type-1 copper formed complexes with NO in the oxidised state, which complexes were only fully formed at low temperatures and could be photodissociated at 77K. Complex formation led to the disappearance of the EPR signal of type-1 copper and of the optical absorbance band in the 600 nm region. In azurin, photodissociation caused the reappearance of the original 625 nm absorbance band, but in the blue oxidases, a new band with lower intensity was found at 595 nm instead of the original absorbance band at 610 nm. In all cases, the EPR signal of type-1 copper did not return. These results are best explained by the formation of a photolabile type-1 Cu1+-NO+ complex. They also indicate that in the complex formed, the type-1 copper structure is probably not disrupted, and that after illumination, the nitric oxide molecule is still in the near vicinity of the copper atom. Type-2 copper did not react at all with nitric oxide, and type-3 copper formed complexes with nitric oxide in both the oxidised and the reduced state, but photodissociation of these complexes could not be demonstrated.
Gorren
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AC
Laboratory of Biochemistry, University of Amsterdam, The Netherlands.
de Boer
E
E
Wever
R
R
D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D001204:Ascorbate Oxidase / Q000032:analysis; D001400:Azurin / Q000378:metabolism; D002570:Ceruloplasmin / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005692:Galactose Oxidase / Q000378:metabolism; D006433:Hemocyanins / Q000378:metabolism; D008667:Metalloproteins / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010782:Photolysis; D013482:Superoxide Dismutase / Q000378:metabolism
D008667:Metalloproteins; D001400:Azurin; D009569:Nitric Oxide; D003300:Copper; D006433:Hemocyanins; D005692:Galactose Oxidase; D001204:Ascorbate Oxidase; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D006631:Amine Oxidase (Copper-Containing)