title: Structure and expression of human thrombomodulin, a thrombin receptor on endothelium acting as a cofactor for protein C activation.
abstract: We have deduced the entire 575-amino acid sequence of the human thrombomodulin precursor from cDNA clones. The precursor starts with an 18-residue signal peptide domain, followed by the NH2-terminal domain, a domain with six epidermal growth factor-like structures, an O-glycosylation site-rich domain, a 24-residue transmembrane domain and a cytoplasmic domain. Simian COS cells transfected with the expression vector pSV2 containing thrombomodulin cDNA synthesized immunoreactive and functionally active thrombomodulin.
authors.affiliation: Department of Laboratory Medicine, Mie University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Kusumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Deyashiki
authors.forename: J
authors.initials: J
authors.lastname: Nishioka
authors.forename: I
authors.initials: I
authors.lastname: Maruyama
authors.forename: M
authors.initials: M
authors.lastname: Zushi
authors.forename: S
authors.initials: S
authors.lastname: Kawahara
authors.forename: G
authors.initials: G
authors.lastname: Honda
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Horiguchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D011486:Protein C / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D018179:Receptors, Thrombin; D012689:Sequence Homology, Nucleic Acid; D013917:Thrombin / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D011486:Protein C; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; D004247:DNA; D013917:Thrombin