[Representation of the leader region of Rous sarcoma virus genome RNA in double-stranded RNA produced in virus-transformed cells].
Restriction fragments of recombinant plasmids containing a proviral sequence of Rous sarcoma virus (RSV) were Southern hybridized with double-stranded (ds) RNA isolated from the cells transformed with RSV. Hybridization data show that the major subpopulation of dsRNA molecules is homologous to the 5'-end region of the viral genome including the leader sequence. We have analysed the RNAs of RSV-transformed cells by the Northern procedure hybridizing them with the proviral fragment containing double long terminal repeats. The results demonstrate that the 14-16S RNA fraction is enriched in sequences which are homologous to the proviral end regions. We consider this RNA fraction to be homologous to the 5'-terminal region of the viral genome and (or) to its antisense strand.
Perevozchikov
A P
AP
Sizonenko
G I
GI
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001483:Base Sequence; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D004262:DNA Restriction Enzymes; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D004262:DNA Restriction Enzymes
Predstavlennost' lidernogo raĭona genomnoĭ RNK virusa sarkomy Rausa v dvukhtsepochechnoĭ RNK, produtsiruemoĭ v kletkakh, transformirovannykh virusom.