Abolition of DNA recognition site resistance to the restriction endonuclease EcoRII.
The EcoRII recognition sites 5'-CCATGG-3' which occur three times in T3 DNA are refractory to this restriction endonuclease. However, these sites are specifically cleaved by EcoRII when a second, susceptible DNA species (pBR 322, phage lambda) is present.
Krüger
D H
DH
Institute of Virology, Humboldt University School of Medicine, Berlin, G.D.R.
Barcak
G J
GJ
Smith
H O
HO
D010582:Bacteriophage lambda / Q000235:genetics; D001665:Binding Sites; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics; D013604:T-Phages / Q000235:genetics*
D004269:DNA, Bacterial; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific