Inactivation of [Leu5]-enkephalin in three isolated preparations: relative importance of aminopeptidase, endopeptidase-24.11 and peptidyl dipeptidase A.
Enkephalin had been shown to be almost exclusively hydrolyzed by three peptidases in the previous studies. In the present investigation, the relative importance of three enzymes in the inactivation of [Leu5]-enkephalin was examined in three isolated preparations. Results showed that amastatin-sensitive aminopeptidase played the greatest role in both guinea-pig ileum and rat vas deferens while it played the similar role to either phosphoramdidon-sensitive endopeptidase-24.11 or captopril-sensitive peptidyl dipeptidase A in mouse vas deferens.
Oka
T
T
Department of Pharmacology, School of Medicine, Tokai University, Isehara, Japan.
Aoki
K
K
Kajiwara
M
M
Ishii
K
K
Kuno
Y
Y
Hiranuma
T
T
Matsumiya
T
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002216:Captopril / Q000494:pharmacology; D004743:Enkephalin, Leucine / Q000378:metabolism*; D006020:Glycopeptides / Q000494:pharmacology; D006168:Guinea Pigs; D006868:Hydrolysis; D007082:Ileum / Q000201:enzymology; D007700:Kinetics; D008297:Male; D008666:Metalloendopeptidases / Q000378:metabolism*; D051379:Mice; D015260:Neprilysin; D009842:Oligopeptides / Q000494:pharmacology; D010455:Peptides; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D014649:Vas Deferens / Q000201:enzymology
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D009842:Oligopeptides; D010455:Peptides; D004743:Enkephalin, Leucine; C016977:amastatin; D002216:Captopril; D007703:Peptidyl-Dipeptidase A; D008666:Metalloendopeptidases; D015260:Neprilysin; C008890:phosphoramidon