title: Characterization of the genome of equine herpesvirus 1 subtype 2.
abstract: The genome structure of equine herpesvirus 1 (EHV-1) subtype 2 was shown by electron microscopic studies and restriction endonuclease site mapping to comprise two covalently linked segments (L, 109 kbp; S, 35 kbp). The S segment contains a unique sequence (US) flanked by a substantial inverted repeat (TRS/IRS). Thus, the genome structure of EHV-1 subtype 2 is similar to that published previously for EHV-1 subtype 1, but the two subtypes differ in the occurrences of EcoRI and BamHI restriction sites. Hybridization studies using cloned EHV-1 DNA showed that the genome of EHV-1 subtype 2 is colinear with the genomes of EHV-1 subtype 1 and herpes simplex virus type 1. DNA sequence data for four EHV-1 subtype 2 genes, including one potentially encoding a glycoprotein, were obtained by sequencing a 4574 bp BamHI fragment containing the junction between US and TRS. The genome structure, hybridization and sequence data confirm that EHV-1 subtype 2 is of the alphaherpesvirus lineage.
authors.affiliation: Medical Research Council Virology Unit, Institute of Virology, U.K.
authors.forename: A A
authors.initials: AA
authors.lastname: Cullinane
authors.forename: F J
authors.initials: FJ
authors.lastname: Rixon
authors.forename: A J
authors.initials: AJ
authors.lastname: Davison
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics / Q000648:ultrastructure; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D004861:Herpesvirus 1, Equid / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral