Assessment by ELISA of passively acquired protection against infectious bursal disease virus in chickens.
The level of maternal antibody to infectious bursal disease (IBD) virus in the circulation of one-day-old layer strain chickens was found to be on average, 45% of the antibody titre in their respective dam, while the minimum ELISA titre which protected against a challenge of 1000 CID50 of virus was 400. Maternal antibody was found to disappear from the circulation of these crossbred chickens with a half-life of 6.7 days. From these data it is possible to estimate the ELISA titre necessary in vaccinated hens to provide the desired duration of passive protection; protection being assessed by an ELISA which measures IBD viral antigen in the bursa of Fabricius following challenge.
Fahey
K J
KJ
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria.
Crooks
J K
JK
Fraser
R A
RA
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D002060:Bursa of Fabricius / Q000276:immunology / Q000473:pathology; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D007112:Immunity, Maternally-Acquired; D007243:Infectious bursal disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology*; D012087:Reoviridae / Q000276:immunology*; D012088:Reoviridae Infections / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral; D000956:Antigens, Viral