[Effect of hyaluronidase on the end-plate miniature currents and potentials in the frog].
The effect of 0.1% hyaluronidase on miniature end-plate potentials and currents (MEPP and MEPC) was studied in the frog cutaneous-pectoris muscle. The action of hyaluronidase on armin-pretreated muscles caused a decrease in the amplitude, duration of half-decay time and rising phase of MEPPs and MEPCs. The positive correlation between the amplitude and half-decay time of MEPPs and MEPCs was diminished. Hyaluronidase treatment of preparations with active acetylcholinesterase caused the half-life time of MEPCs to increase without any changes in the amplitude and rising phase of MEPCs. It is suggested that enzymatic destruction of a part of the glycocalix of cells forming the neuromuscular junction and of a part of the extracellular matrix results in a weakening of the nonspecific acetylcholine binding, thus facilitating the acetylcholine diffusion into the synaptic cleft.
Vinogradova
I M
IM
Matiushkin
D P
DP
D000818:Animals; D001138:Armin / Q000494:pharmacology; D002800:Cholinesterase Inhibitors; D004553:Electric Conductivity; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D009045:Motor Endplate / Q000187:drug effects* / Q000502:physiology; D009469:Neuromuscular Junction / Q000187:drug effects*; D011898:Ranidae; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology
D002800:Cholinesterase Inhibitors; D017981:Receptors, Neurotransmitter; D006821:Hyaluronoglucosaminidase; D001138:Armin
Vliianie gialuronidazy na miniatiurnye potentsialy i toki kontsevoĭ plastinki liagushki.