Superoxide-induced deimination of arginine in hematopoietic cells.
Murine bone marrow cells can produce citrulline directly from L-arginine without intermediate ornithine. An L-arginine-dependent biochemical pathway synthesizing L-citrulline and nitrate, coupled to an effector mechanism has also been recently demonstrated in murine cytotoxic activated macrophages. We show herein that L-citrulline synthesis in murine bone marrow cells can be induced by the generation of superoxide. It can take place in an arginine-free medium, suggesting the implication of a superoxide-dependent peptidyl arginine deiminase.
Kamoun
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PP
Laboratoire de Biochimie Génétique, Hôpital Necker-Enfants Malades, Paris, France.
Schneider
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E
Dy
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M
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001853:Bone Marrow / Q000378:metabolism; D002956:Citrulline / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D007097:Imines; D051379:Mice; D013481:Superoxides / Q000378:metabolism*
D007097:Imines; D013481:Superoxides; D002956:Citrulline; D001120:Arginine