Murine thymomas induced by fractionated-X-irradiation have specific T-cell receptor rearrangements and characteristics associated with day-15 to -16 fetal thymocytes.
We report here that specific T-cell receptor rearrangements were observed in fractionated-X-irradiation-induced murine leukemias. Consistent gamma-chain rearrangements, limited beta-chain rearrangements, and no detectable alpha-chain rearrangements were observed. Gene expression studies revealed that, in comparison with normal thymus tissue, expression of alpha T-cell receptor genes was lower in the thymomas, beta expression was much higher but approximately equal to that of normal thymocytes, and gamma expression was significantly increased. After coupling these data with those from analyses using reagents against other surface markers, such as Lyt-2, L3T4, H-2, IL-2R and MEL-14, we concluded that the target T cells for fractionated-X-irradiation-induced transformation resemble fetal thymocytes from days 15 and 16 of gestation.
Amari
N M
NM
Department of Pathology, New York University Medical Center, New York 10016.
Meruelo
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D
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics; D005786:Gene Expression Regulation; D005865:Gestational Age; D051379:Mice; D008810:Mice, Inbred C57BL; D009381:Neoplasms, Radiation-Induced; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013945:Thymoma / Q000209:etiology / Q000235:genetics*; D013950:Thymus Gland / Q000196:embryology* / Q000378:metabolism; D013953:Thymus Neoplasms / Q000209:etiology / Q000235:genetics*; D013997:Time Factors
D000954:Antigens, Surface; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D011948:Receptors, Antigen, T-Cell; D004262:DNA Restriction Enzymes