Growth factors and receptors of lymphocytes.
To understand molecular mechanisms of clonal expansion of lymphocytes we have isolated cDNA clones for two lymphokines, interleukins (IL) 4 and 5 that induce proliferation and maturation of B-lymphocytes. Structures of IL-4 and IL-5 revealed a remote homology with other lymphokines such as IL-3 and gamma-interferon. IL-4 and IL-5 were shown to affect not only B-lymphocytes but also T-lymphocytes and several other cells derived from bone marrow stem cells. We have also studied the structure and function of the IL-2 receptor: our focus was the molecular basis for the high and low affinity states of the receptor encoded by the identical cDNA. We propose the affinity conversion model that the high-affinity state of the IL-2 receptor is a ternary complex of IL-2, the IL-2 receptor, and a postulated "converter protein", which is fewer in number than the receptors.
Kinashi
T
T
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
Noma
Y
Y
Azuma
C
C
Kondo
S
S
Tanabe
T
T
Konishi
M
M
Kinoshita
M
M
Takahashi
M
M
Saito
Y
Y
Ogura
T
T
D000818:Animals; D002455:Cell Division; D003001:Cloning, Molecular; D004247:DNA; D005815:Genetic Code; D006133:Growth Substances / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D015847:Interleukin-4; D015848:Interleukin-5; D007378:Interleukins / Q000235:genetics; D008214:Lymphocytes / Q000166:cytology* / Q000502:physiology; D008954:Models, Biological; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism; D015375:Receptors, Interleukin-2
D006133:Growth Substances; D015848:Interleukin-5; D007378:Interleukins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D015847:Interleukin-4; D004247:DNA