[Effective selection and analysis of IS1 element transposition using the oLpLN region of phage lambda].
The plasmid pNT6 permits selection of IS1-element insertions into the plasmid occurring with the frequency about 10%. The bacteriophage promoter pL cloned in pNT6 is a hot-spot region for IS1-element insertion. The frequency of IS1 transposition into pL depends on genotype. The plasmid pNT6 may be considered to be a useful target DNA for screening and analysis of IS1-element transposition.
Glukhov
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IL
Fodor
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I
D010582:Bacteriophage lambda / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014169:Transformation, Bacterial
D004251:DNA Transposable Elements
Effektivnyĭ otbor i analiz transpozitsiĭ IS1-élementa s pomoshch'iu oblasti oLpLN faga lambda.