Residual bodies in the retinal pigment epithelium induced by intravitreal netilmicin.
Intravitreal injection of aminoglycoside antibiotics is known to induce morphological changes in the retinal pigment epithelium (RPE) resembling a lipidosis. The RPE of netilmicin-treated rabbits displays a dose-related increase in autofluorescence compared to untreated controls. Netilmicin produces an accumulation of membrane-limited osmiophilic lamellated inclusions in the pigment epithelial cell. These inclusions measure from 1 to 3 microns in diameter, and have acid hydrolase activity demonstrated by cytidine monophosphate cytochemistry. These findings suggest that netilmicin-induced inclusions are residual bodies and that the accumulation of these residual bodies is responsible for the observed cellular lipidosis.
Tabatabay
C A
CA
Howe Laboratory of Ophthalmology, Harvard Medical School, Boston, Massachusetts.
D'Amico
D J
DJ
Hanninen
L A
LA
Casey
V N
VN
Kenyon
K R
KR
D000818:Animals; D006651:Histocytochemistry; D006867:Hydrolases / Q000378:metabolism; D002479:Inclusion Bodies / Q000201:enzymology / Q000648:ultrastructure*; D007267:Injections; D008856:Microscopy, Fluorescence; D009428:Netilmicin / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000648:ultrastructure*; D011817:Rabbits; D012160:Retina / Q000201:enzymology; D014822:Vitreous Body
D009428:Netilmicin; D006867:Hydrolases