Diurnal variation in 5'-nucleotidase activity in rat liver. A quantitative histochemical study.
The diurnal variation of 5'-nucleotidase activity in periportal and pericentral areas of rat liver parenchyma has been determined with quantitative histochemical means. 5'-Nucleotidase activity was estimated using microdensitometry in cryostat sections after being incubated with a medium according to Wachstein and Meisel (1957). It appeared that 5'-nucleotidase activity was significantly higher in pericentral areas than in periportal areas throughout the daily cycle and showed a maximum at the end of the light period. It was concluded that 5'-nucleotidase activity may be related with the capacity to diminish messenger RNA resulting in protein breakdown.
Frederiks
W M
WM
University of Amsterdam, Academic Medical Centre, The Netherlands.
Marx
F
F
Bosch
K S
KS
Van Noorden
C J
CJ
D015720:5'-Nucleotidase; D000818:Animals; D002940:Circadian Rhythm; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology*; D008297:Male; D009708:Nucleotidases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry
D009708:Nucleotidases; D015720:5'-Nucleotidase