A study of free radicals in paraffin embedded and deparaffinized human heart muscle tissue using electron spin resonance (ESR).
Free radicals (spectroscopic splitting factor; g factor = 2.003-2.005) were investigated in formol-fixed, paraffin embedded heart-muscle tissue sections using electron spin resonance (ESR) spectra. Changes in signal amplitude, g factor and line width were registered during deparaffinization, chloroform-methanol extraction, vapour treatment and bromination. An attempt was made to identify the source of the ESR signals by a correlation between the signal amplitude and number of fluorescent and/or Sudan-black-positive granules counted in the tissue sections. An increase in signal amplitude, g value and line narrowing were characteristic of the ascorbyl radical after deparafinization in air. Vapour treatment revelated that the broader signal has lower g factor, a characteristic that is tentatively assigned to oxidized lipids. The bromination resistant minor fraction of free radical centres with small g factor might be associated with the pigment content of the samples.
Elek
G
G
Department of Pathology, Railway Hospital, Budapest, Hungary.
Rockenbauer
A
A
Magyar
E
E
Jókay
L
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism; D001966:Bromine; D002675:Child, Preschool; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005260:Female; D005609:Free Radicals; D006801:Humans; D007231:Infant, Newborn; D008062:Lipofuscin / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000032:analysis*; D010232:Paraffin
D005609:Free Radicals; D008062:Lipofuscin; D010232:Paraffin; D001966:Bromine