title: The molecular organization of the lysostaphin gene and its sequences repeated in tandem.
abstract: The gene encoding lysostaphin of Staphylococcus staphylolyticus was cloned in Escherichia coli and its DNA sequence was determined. The complete coding region comprises 1440 base pairs corresponding to a precursor of 480 amino acids (molecular weight 51 669). It was shown by NH2-terminal amino acid sequence analysis of the purified extracellular lysostaphin from S. staphylolyticus that the mature lysostaphin consists of 246 amino acid residues (molecular weight 26926). Polyacrylamide gel electrophoresis revealed a similar molecular weight for the most active form. By computer analysis the secondary protein structure was predicted. It revealed three distinct regions in the precursor protein: a typical signal peptide (ca. 38 aa), a hydrophilic and highly ordered protein domain with 14 repetitive sequences (296 aa) and the hydrophobic mature lysostaphin. The lysostaphin precursor protein appears to be organized as a preprolysostaphin.
authors.affiliation: Institut für Biochemie der Universität München, Federal Republic of Germany.
authors.forename: P
authors.initials: P
authors.lastname: Heinrich
authors.forename: R
authors.initials: R
authors.lastname: Rosenstein
authors.forename: M
authors.initials: M
authors.lastname: Böhmer
authors.forename: P
authors.initials: P
authors.lastname: Sonner
authors.forename: F
authors.initials: F
authors.lastname: Götz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008248:Lysostaphin / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D013210:Staphylococcus / Q000235:genetics*
chemical_list: D003062:Codon; D004262:DNA Restriction Enzymes; D008248:Lysostaphin