Distinct autoradiographic labelling of serotonin 5-HT2 receptors, alpha 1-adrenoceptors and histamine-H1 receptors and of tetrabenazine-displaceable ketanserin binding sites in rodent brain with [125I]7-amino-8-iodo-ketanserin.
[125I]7-Amino-8-iodo-ketanserin ([125I]AMIK) binding was assayed in rat and guinea-pig horizontal brain sections and was analysed by autoradiography. The use of selective displacers allowed the identification of four distinct receptors in rat and guinea-pig brain. In rats, 5-HT2 receptors were detected in cortical areas and in caudate putamen, alpha 1-adrenoceptors were found in cortical areas and several thalamic nuclei, tetrabenazine-displaceable ketanserin binding sites involved in the release of biogene amine metabolites occurred in caudate putamen, periaqueductal grey matter and septal nuclei. Histamine-H1 receptors could be identified in the guinea-pig cerebellum.
Schotte
A
A
Department of Biochemical Pharmacology, Janssen Research Foundation, Beerse, Belgium.
Leysen
J E
JE
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D006168:Guinea Pigs; D007457:Iodine Radioisotopes; D007650:Ketanserin / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D011968:Receptors, Histamine / Q000378:metabolism*; D011969:Receptors, Histamine H1 / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000378:metabolism*; D013747:Tetrabenazine / Q000494:pharmacology
D007457:Iodine Radioisotopes; D011942:Receptors, Adrenergic, alpha; D011968:Receptors, Histamine; D011969:Receptors, Histamine H1; D011985:Receptors, Serotonin; C050315:7-amino-8-iodoketanserin; D007650:Ketanserin; D013747:Tetrabenazine