Levels of glutathione S transferase pi mRNA in human lung cancer cell lines correlate with the resistance to cisplatin and carboplatin.
The amounts of mRNA for glutathione S transferase pi (GST pi) were significantly lower in 3 human small cell lung cancer (SCLC) cell lines than in 3 non small cell lung cancer (NSCLC) cell lines. The sensitivities of the 3 SCLC cell lines to cisplatin and carboplatin were much higher than those of the 3 NSCLC cell lines. These results indicate that low levels of GST pi mRNA expression in SCLC cell lines inversely correlate to high sensitivity to cisplatin and carboplatin, and further suggest that GST pi may play an important role in intracellular inactivation of these drugs.
Nakagawa
K
K
Pharmacology Division, National Cancer Center Research Institute, Tokyo.
Yokota
J
J
Wada
M
M
Sasaki
Y
Y
Fujiwara
Y
Y
Sakai
M
M
Muramatsu
M
M
Terasaki
T
T
Tsunokawa
Y
Y
Terada
M
M
D016190:Carboplatin; D018288:Carcinoma, Small Cell / Q000188:drug therapy / Q000201:enzymology* / Q000378:metabolism; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology*; D004351:Drug Resistance; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000378:metabolism; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism
D009944:Organoplatinum Compounds; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016190:Carboplatin; D005982:Glutathione Transferase; D002945:Cisplatin