Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method.
We describe a simple and effective method for inhibition of endogenous peroxidase activity in the immunoperoxidase technique. Specimens are pre-treated with a mixture of azide and hydrogen peroxide, which is then followed by an indirect immunoperoxidase procedure. Comparison studies showed no significant loss of antigenicity or morphological details by this pre-treatment. The method is most useful for evaluating cell-specific antigens on specimens that have abundant endogenous peroxidase activity, such as blood and bone marrow.
Li
C Y
CY
Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.
Ziesmer
S C
SC
Lazcano-Villareal
O
O
D001386:Azides; D001773:Blood Cells / Q000276:immunology; D001854:Bone Marrow Cells; D006801:Humans; D006861:Hydrogen Peroxide; D007124:Immunoenzyme Techniques; D009195:Peroxidase / Q000037:antagonists & inhibitors*
D001386:Azides; D006861:Hydrogen Peroxide; D009195:Peroxidase