The influence of virus transformation and cell population density on some membrane properties of mouse fibroblasts in culture.
The influence of cell population density and simian virus 40 transformation on the activity of the Na-K pump was studied in mouse fibroblasts cultured in medium supplemented with fetal bovine serum. The activity of the Na-K pump was determined from K+ influx, ethacrynate-sensitive K+ influx, (Na+ + K+)-ATPase assay, and the determinations of intracellular potassium and sodium ion concentrations in these cells. The activity of the Na-K pump was found to decrease in density-inhibited cultures of normal fibroblasts (designated as 3T3 cells), while in the virus-transformed cells (SV3T3) the activity remained fairly constant at all cell population densities.
Tenang
E M
EM
Department of Physiology and Pharmacology, University of St. Andrews, United Kingdom.
McCaldin
B
B
D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004976:Ethacrynic Acid / Q000032:analysis; D005347:Fibroblasts; D051379:Mice; D011188:Potassium / Q000032:analysis / Q000378:metabolism; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000032:analysis / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis
D012414:Rubidium Radioisotopes; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D004976:Ethacrynic Acid; D011188:Potassium