A human glioblastoma line with karyotypic nullisomy 13 containing several chromosome 13-specific sequences.
Using a biochemical approach (evaluation of esterase D activity) and recombinant DNA techniques (in situ and filter hybridization with specific DNA probes) a glioblastoma cell line with karyotypical nullisomy 13 was shown to contain several chromosome #13-specific sequences. They were assigned to a marker chromosome. This finding suggests that cytogenetic descriptions of deletions or chromosome losses will gain considerable power of the statement when supplemented by molecular analysis, particularly since, by now, DNA probes have been accumulated for all chromosomes and all their subfragments.
Zang
K D
KD
Institute of Human Genetics, University of the Saar, Homburg, F.R.G.
Fischer
H
H
van der Hout
A
A
Unteregger
G
G
Henn
W
W
Scheffer
H
H
Wollenberg
C
C
Buys
C H
CH
Blin
N
N
D002871:Chromosome Banding; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005819:Genetic Markers; D005910:Glioma / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D014407:Tumor Cells, Cultured
D005819:Genetic Markers; D004247:DNA; D004262:DNA Restriction Enzymes