Synergistic action of interferons (IFN-alpha/beta and IFN-gamma) of different species origin.
The effects of the mixtures of human, porcine and murine interferons (IFNs) alpha, beta and gamma and bovine IFN-alpha were studied in human, porcine and murine cells. The synergistic action of the mixtures of IFN-alpha/beta and IFN-gamma occurred not only in the homologous but also in the heterologous systems. The synergistic effect was observed when one of the IFNs was of alpha/beta type and the other one of gamma type and when both of them were active in the target cells, regardless of their species origin. Because PoIFN-alpha is active in the cells of numerous mammalian species, it is a good tool for the studies on the synergistic effects. The synergistic action of the mixture of IFNs was abolished after inactivation of one of the IFNs. Specific inactivation of IFN-alpha/beta in the mixture was accomplished by incubation with anti-IFN sera and in the case of IFN-gamma, by acidifying to pH 2.
Piasecki
E
E
Laboratory of Tumor Virology, Polish Academy of Sciences, Wroclaw.
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004357:Drug Synergism; D006801:Humans; D007370:Interferon Type I / Q000008:administration & dosage*; D007371:Interferon-gamma / Q000008:administration & dosage*; D051379:Mice; D013045:Species Specificity; D013552:Swine; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects*
D007370:Interferon Type I; D007371:Interferon-gamma