Determination of delta 9-tetrahydrocannabinol in human blood and saliva by high-performance liquid chromatography with amperometric detection.
A rapid, sensitive and selective determination of delta 9-tetrahydrocannabinol (THC) in human plasma, serum and saliva was developed with high-performance liquid chromatography with electrochemical detection. Initially, samples were deproteinized, followed by a one step liquid-liquid extraction. Samples were measured by high-performance liquid chromatography with electrochemical detection with 4-dodecylresorcinol as the internal standard. The minimal detectable limit for THC in biological samples was ca. 1 ng/ml with a signal-to-noise ratio greater than 3, corresponding to an on-column sensitivity for THC of ca. 0.5 ng. The detector was operated at + 0.90 V vs. Ag/AgCl and exhibited linearity over a concentration range of 1-150 ng/ml with correlation coefficients of the standard curves greater than 0.99.
Thompson
L K
LK
NIDA Addiction Research Center, Baltimore, MD 21224.
Cone
E J
EJ
D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000032:analysis* / Q000097:blood; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D008385:Marijuana Smoking; D012463:Saliva / Q000032:analysis
D007202:Indicators and Reagents; D013759:Dronabinol