An effective approach to the identification of subclones of a DNA of known nucleotide sequence.
The problem of subclone identification for DNA fragments of a known nucleotide sequence has been considered. We suggest a strategy for rapid identification of a large number of subclones based on: (i) partial sequencing of the subclone DNA (single nucleotide track); (ii) representation of the result in the form of a numeric code showing the distribution of the chosen nucleotide along the sequence; and (iii) identification of the subclone sequence using this code in a catalogue compiled and printed for a whole DNA sequence. The same approach is applicable when the subclones are expected to have homology with known sequences.
Golovanov EuI
Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.
Nedospasov
S A
SA
D000465:Algorithms; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004279:DNA, Viral; D001330:Electronic Data Processing; D013539:Simian virus 40 / Q000235:genetics
D004279:DNA, Viral; D004247:DNA