Monoclonal antibodies against herpes simplex virus type 1-infected nuclei defining and localizing the ICP8 protein, 65K DNA-binding protein and polypeptides of the ICP35 family.
The production and properties of monoclonal antibodies raised against herpes simplex virus type 1 (HSV-1)-infected cell nuclei are described. Biological and immunochemical assays revealed that these antibodies recognize four different proteins in HSV-1-infected cells. Four antibodies reacted with the major DNA-binding protein (ICP8) and six with the 65K DNA-binding protein. Two antibodies detected the ICP35 family of proteins and one antibody bound to a protein with an apparent mol. wt. of 60K. Immune electron microscopy showed that the major DNA-binding protein had a patchy distribution, whereas the 65K DNA-binding protein was evenly spread in the infected cell nuclei. The 60K protein as well as the polypeptides of the ICP35 family were preferentially found associated with the viral capsid.
Schenk
P
P
Institut für Virologie der Freien Universität Berlin, F.R.G.
Pietschmann
S
S
Gelderblom
H
H
Pauli
G
G
Ludwig
H
H
Schenck
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis; D002467:Cell Nucleus / Q000276:immunology / Q000382:microbiology*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000276:immunology*; D006825:Hybridomas; D007118:Immunoassay; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D018139:Simplexvirus / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004268:DNA-Binding Proteins; C054582:ICP35 protein, Human herpesvirus 1; C045462:ICP8 protein, Simplexvirus; D014764:Viral Proteins