Microinjection of catalytic subunit of cyclic AMP-dependent protein kinase triggers acute morphological changes in thyroid epithelial cells.
In dog thyroid epithelial cells in primary culture, thyrotropin acting through cyclic AMP induced rapid morphological changes associated with complete disruption of actin containing stress fibers. This modification preceded cell retraction and rounding up. These morphological effects were also induced by glass capillary microinjection of purified catalytic subunit of cAMP-dependent protein kinase. This provides the first direct evidence in intact cells that catalytic subunit, which is released upon activation of cAMP-dependent protein kinases, is responsible for cAMP-dependent morphological transformation.
Roger
P P
PP
Institut de Recherche Interdisciplinaire, Brussels, Belgium.
Rickaert
F
F
Huez
G
G
Authelet
M
M
Hofmann
F
F
Dumont
J E
JE
D000199:Actins / Q000378:metabolism; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002384:Catalysis; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D004285:Dogs; D004789:Enzyme Activation; D004848:Epithelium / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008845:Microinjections; D011494:Protein Kinases / Q000378:metabolism / Q000494:pharmacology*; D013961:Thyroid Gland / Q000187:drug effects / Q000648:ultrastructure*; D013972:Thyrotropin / Q000494:pharmacology
D000199:Actins; D005576:Colforsin; D003994:Bucladesine; D013972:Thyrotropin; D000242:Cyclic AMP; D011494:Protein Kinases