Detection of human papillomavirus-6 in primary carcinoma of the urethra in men.
The authors report a case of primary urethral carcinoma which seems to be linked to human papillomavirus (HPV) infection. Southern blot hybridization of phosphorus 32 (32P)-labeled DNA extracted from the cancer tissue gave a positive reaction with HPV-6. Specimens of the tumor material subjected to in situ hybridization with 3H-labeled HPV-6 DNA showed numerous tumor cell clusters with clearly labeled nuclei. Using immunoperoxidase staining the authors found papillomavirus structural antigen within cell nuclei of the tumor tissue.
Grussendorf-Conen
E I
EI
Department of Dermatology, University Hospital, Aachen, West Germany.
Deutz
F J
FJ
de Villiers
E M
EM
D000956:Antigens, Viral / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D014412:Tumor Virus Infections / Q000175:diagnosis*; D014523:Urethral Neoplasms / Q000209:etiology / Q000382:microbiology*
D000956:Antigens, Viral; D004279:DNA, Viral