title: Interrenal activity in the female lizard Lacerta vivipara J.: in vitro response to ACTH 1-39 and to [Sar1, Val5] angiotensin II (ANG II).
abstract: A perifusion system technique was developed in order to determine in vitro the respective roles of ACTH and ANG II in the regulation of adrenal steroidogenesis in the lizard Lacerta vivipara. Synthetic human ACTH 1-39, administered as 20-min pulses, stimulated corticosterone (B) and aldosterone (A) release in a dose-dependent manner. The increase in corticosterone output was higher than that in aldosterone output, leading to an enhancement of the B/A ratio. Iterative stimulations with 1 nM ACTH (20-min pulses every 120 min) led to reproducible increases in corticosterone and aldosterone release. Prolonged stimulation with 1 nM ACTH (up to 240 min) caused a sustained increase in corticosteroid release, suggesting that, in the lizard, ACTH does not induce any desensitization phenomenon. The angiotensin II analogue [Sar1, Val5] ANG II also stimulated corticosterone and aldosterone release in a dose-dependent manner; the stimulatory effects of ANG II on both steroids were very similar. These results indicate that, in lizards, ACTH plays a major role in the regulation of adrenal steroidogenesis. Since ANG II stimulates the production of gluco- and mineralocorticoids, our data raise the question of the existence of two cell types synthesizing corticosterone and aldosterone, respectively, in reptiles.
authors.affiliation: Ecole Normale Supérieure, Biochimie et Physiologie du Développement, CNRS-UA 686, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Dauphin-Villemant
authors.forename: F
authors.initials: F
authors.lastname: Leboulenger
authors.forename: F
authors.initials: F
authors.lastname: Xavier
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000450:Aldosterone / Q000096:biosynthesis*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D003345:Corticosterone / Q000096:biosynthesis*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008116:Lizards / Q000378:metabolism*
chemical_list: D000804:Angiotensin II; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone