title: Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus.
abstract: A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5' termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.
authors.affiliation: Department of Microbiology, University of Alberta, Edmonton, Canada.
authors.forename: C L
authors.initials: CL
authors.lastname: Keen
authors.forename: S
authors.initials: S
authors.lastname: Mendelovitz
authors.forename: G
authors.initials: G
authors.lastname: Cohen
authors.forename: Y
authors.initials: Y
authors.lastname: Aharonowitz
authors.forename: K L
authors.initials: KL
authors.lastname: Roy
mesh_terms: D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes