title: The influence of virus transformation and cell population density on some membrane properties of mouse fibroblasts in culture.
abstract: The influence of cell population density and simian virus 40 transformation on the activity of the Na-K pump was studied in mouse fibroblasts cultured in medium supplemented with fetal bovine serum. The activity of the Na-K pump was determined from K+ influx, ethacrynate-sensitive K+ influx, (Na+ + K+)-ATPase assay, and the determinations of intracellular potassium and sodium ion concentrations in these cells. The activity of the Na-K pump was found to decrease in density-inhibited cultures of normal fibroblasts (designated as 3T3 cells), while in the virus-transformed cells (SV3T3) the activity remained fairly constant at all cell population densities.
authors.affiliation: Department of Physiology and Pharmacology, University of St. Andrews, United Kingdom.
authors.forename: E M
authors.initials: EM
authors.lastname: Tenang
authors.forename: B
authors.initials: B
authors.lastname: McCaldin
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004976:Ethacrynic Acid / Q000032:analysis; D005347:Fibroblasts; D051379:Mice; D011188:Potassium / Q000032:analysis / Q000378:metabolism; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000032:analysis / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis
chemical_list: D012414:Rubidium Radioisotopes; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D004976:Ethacrynic Acid; D011188:Potassium