title: A captive solvent method for rapid N-[11C]methylation of secondary amides: application to the benzodiazepine, 4'-chlorodiazepam (RO5-4864).
abstract: [11C]4'-Chlorodiazepam (RO5-4864), for PET studies of peripheral benzodiazepine receptors, was synthesized by alkylation of 1-desmethyl-4'-chlorodiazepam, in a small volume of acetone adsorbed on acrylic yarn, with [11C]methyl iodide in the injection loop of a liquid chromatograph. The reaction mixture was introduced directly onto a small, disposable alumina chromatographic column. Elution with pentane:ethanol gave a product of high chemical and radiochemical purity. A simple heating and cooling device for the injection loop is described.
authors.affiliation: Cyclotron/PET Facility, University of Michigan Medical School, Ann Arbor 48109-0552.
authors.forename: G L
authors.initials: GL
authors.lastname: Watkins
authors.forename: D M
authors.initials: DM
authors.lastname: Jewett
authors.forename: G K
authors.initials: GK
authors.lastname: Mulholland
authors.forename: M R
authors.initials: MR
authors.lastname: Kilbourn
authors.forename: S A
authors.initials: SA
authors.lastname: Toorongian
mesh_terms: D001570:Benzodiazepinones; D002250:Carbon Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D013997:Time Factors; D014055:Tomography, Emission-Computed
chemical_list: D001570:Benzodiazepinones; D002250:Carbon Radioisotopes; C028513:4'-chlorodiazepam