title: Preferential expression of VH5 and VH6 immunoglobulin genes in early human B-cell ontogeny.
abstract: Analysis of Ig transcripts by dot blot hybridization revealed that VH5 and VH6 RNA first occurred during the 7th week of gestation in human fetal liver. At the same time, a C lambda or C lambda-like chain was also expressed, in the absence of detectable kappa chains. In adult B cells, VH3 appears to be the dominant family expressed. Because VH5 and VH6 were located as the most proximal V genes to the JH cluster, the orderly expression of human VH genes seems to resemble the situation reported for the mouse.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Cuisinier
authors.forename: V
authors.initials: V
authors.lastname: Guigou
authors.forename: L
authors.initials: L
authors.lastname: Boubli
authors.forename: M
authors.initials: M
authors.lastname: Fougereau
authors.forename: C
authors.initials: C
authors.lastname: Tonnelle
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D015342:DNA Probes; D005333:Fetus; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D015151:Immunoblotting; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008099:Liver / Q000196:embryology / Q000276:immunology; D012333:RNA, Messenger / Q000302:isolation & purification
chemical_list: D015342:DNA Probes; D007135:Immunoglobulin Variable Region; D012333:RNA, Messenger