title: Size-uniform heparin fragments as nuclear decondensation and acrosome reaction inducers in human spermatozoa.
abstract: Using size- uniform mixtures of di-, tetra-, octa- and decasaccharides obtained from the depolymerization of heparin with heparinase, we have studied the activity that these low molecular weight heparin fragments may have on the acrosome reaction and sperm nuclei decondensation processess. Swelling of human spermatozoa nuclei was stimulated by heparin and their fragments and was dependent on the incubation time and directly correlated with the size of the fragment tested. Disaccharides were unable to increase the number of swollen nuclei. At short times (2-8 hrs) decasaccharides were the most active substances tested, including heparin. Only heparin and the tetra- and decasaccharides showed a significant increase in the number of acrosome-reacted spermatozoa, both fragments were more active than heparin at 2 hour incubation. Hexa- and octasaccharides induced a slight increase in the number of acrosome reacted spermatozoa and disaccharides were ineffective. The presence in animal systems of oligosaccharides derived from macromolecules and having specific biochemical properties, remembers the recent discovery of to those mediated by oligosaccharins in plants may exist in animals.
authors.affiliation: División de Biología del Desarrollo, Unidad de Investigación Biomédica de Occidente, I.M.S.S., Guadalajara, Jalisco Mexico.
authors.forename: N M
authors.initials: NM
authors.lastname: Delgado
authors.forename: R
authors.initials: R
authors.lastname: Reyes
authors.forename: J
authors.initials: J
authors.lastname: Mora-Galindo
authors.forename: A
authors.initials: A
authors.lastname: Rosado
mesh_terms: D000177:Acrosome / Q000187:drug effects* / Q000502:physiology; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D019763:Heparin Lyase; D006495:Heparin, Low-Molecular-Weight / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008297:Male; D009844:Oligosaccharides / Q000494:pharmacology; D011133:Polysaccharide-Lyases / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure*
chemical_list: D006495:Heparin, Low-Molecular-Weight; D009844:Oligosaccharides; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase