Reactivity of resistance blood vessels ex vivo after administration of toxic chemicals to laboratory animals: arteriolotoxicity.
Three vasotoxic metals (arsenic, lead, cadmium) were investigated for their arteriolotoxicity in rats. A perfused hindleg preparation from control and treated animals was used to determine the vasoreactivity to norepinephrine. After 2 and 4 weeks of treatment with arsenic trioxide (15 mg/kg p.o.) and after 4 weeks of treatment with lead acetate (100 mg/kg p.o.), there was a significant reduction in vasoreactivity (10 20%) expressed as the maximum increase in perfusion pressure (EAmax). A single dose of cadmium chloride (1 mg/kg i.p.) caused a non-significant reduction of 12% in EAmax. We conclude that this method is suitable for detecting arteriolotoxic effects of chemicals in small laboratory animals.
Bekemeier
H
H
Department of Pharmacology, Martin-Luther-University, Halle-Wittenberg, G.D.R.
Hirschelmann
R
R
D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D000077237:Arsenic Trioxide; D001152:Arsenicals; D001158:Arteries; D001160:Arterioles / Q000187:drug effects*; D001794:Blood Pressure / Q000187:drug effects; D001808:Blood Vessels / Q000187:drug effects*; D002104:Cadmium / Q000633:toxicity*; D019256:Cadmium Chloride; D005260:Female; D006614:Hindlimb / Q000098:blood supply; D009638:Norepinephrine; D009942:Organometallic Compounds / Q000633:toxicity*; D010087:Oxides; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D014655:Vascular Resistance / Q000187:drug effects
D001152:Arsenicals; D009942:Organometallic Compounds; D010087:Oxides; D002104:Cadmium; D019256:Cadmium Chloride; D001151:Arsenic; C008261:lead acetate; D000077237:Arsenic Trioxide; D009638:Norepinephrine