Iron-molybdenum cofactor of nitrogenase: electrochemical determination of the electron stoichiometry of the oxidized/semi-reduced couple.
The number of electrons involved in the more positive of the two redox couples of the iron-molybdenum cofactor of Azotobacter vinelandii nitrogenase has been investigated by controlled potential coulometry in both the oxidizing and reducing directions. A n value of 1 was determined for interconversion of the oxidized and semi-reduced states of the cofactor. This electron count was confirmed by double integration of the S = 3/2 electron paramagnetic resonance signal exhibited by the semi-reduced state.
Schultz
F A
FA
Department of Chemistry, Indiana University-Purdue University, Indianapolis 46223.
Gheller
S F
SF
Newton
W E
WE
D001395:Azotobacter / Q000201:enzymology; D004563:Electrochemistry; D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins / Q000378:metabolism; D008983:Molybdoferredoxin / Q000378:metabolism; D009591:Nitrogenase; D010084:Oxidation-Reduction
D005288:Ferredoxins; D008983:Molybdoferredoxin; D009591:Nitrogenase