Potentiation of radiation toxicity in C-6 glioma cells by prostaglandin D2.
The effects of prostaglandin D2 (PG D2) and X-ray radiation on the growth and cell survival of C-6 rat glioma cells were evaluated. Exponentially growing monolayer cultures of C-6 glioma cells were incubated with various concentrations of PG D2 for 24 hours prior to X-ray irradiation. PG D2 exposure was associated with an inhibition of cell growth and a reduction in cell survival (IC50 = 5.2 micrograms/ml). The combination of PG D2 and X-ray radiation significantly increased cell killing in comparison with either PG D2 or X-alone. The enhancing factor measured at a survival level of 10% was determined. PG D2 potentiation of radiation toxicity could be related to a blockage of a G1 phase of the cell cycle.
Tejedor
M
M
Radiotherapeutic Oncology Unit, Hospital de Nuestra Señora del Pino, Las Palmas, Spain.
Sinues
E
E
Alcala
A
A
Conde
B
B
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005910:Glioma; D015230:Prostaglandin D2 / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents; D051381:Rats; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D014965:X-Rays
D011838:Radiation-Sensitizing Agents; D015230:Prostaglandin D2