20-Hydroxyecdysone induces the expression of one beta-tubulin gene in Drosophila Kc cells.
The expression of 56D and 60C beta-tubulin genes has been examined in Drosophila melanogaster Kc cells in response to the insect moulting hormone, 20-hydroxyecdysone (20-OH-E). Northern blots probed with beta-tubulin subclones show that the 56D beta-tubulin gene encodes a 1.8 kb mRNA whose abundance is not affected by 20-OH-E. The 60C gene probe detects two mRNAs: one of 1.8 kb present in untreated and 20-OH-E-treated cells, and one of 2.6 kb present only in 20-OH-E-treated cells; using a 60C 3'-specific probe, only the 2.6 kb is revealed. Hybrid selection translation experiment demonstrates that a 20-OH-E-inducible mRNA homologous to the 60C gene encodes a beta-tubulin subunit (P4); this subunit is the so-called beta 3-tubulin. Translation of size-fractionated mRNA shows that the 20-OH-E-induced beta 3-tubulin subunit is encoded, in treated cells, by the 2.6 kb mRNA.
Montpied
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Laboratoire de Biochimie Médicale, U.A. C.N.R.S. 360, Faculté de Médecine, Clermont-Ferrand, France.
Sobrier
M L
ML
Chapel
S
S
Couderc
J L
JL
Dastugue
B
B
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004331:Drosophila melanogaster; D004441:Ecdysterone / Q000494:pharmacology*; D005796:Genes / Q000187:drug effects*; D046911:Macromolecular Substances; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects*; D014404:Tubulin / Q000235:genetics*
D046911:Macromolecular Substances; D012333:RNA, Messenger; D014404:Tubulin; D004441:Ecdysterone; D004262:DNA Restriction Enzymes