title: Evaluation of methods for detecting human papillomavirus deoxyribonucleotide sequences in clinical specimens.
abstract: Specimens from 26 condylomatous lesions, 24 invasive cancer cells, and 33 cervices, without evidence of the diseases, were tested for the presence of human papillomavirus (HPV) types 6, 11, 16, and 18 by Southern blot hybridization, in situ filter hybridization, or in situ tissue hybridization methods. A total of 89% (23 of 26) of the condylomatous lesions contained HPV DNAs, as determined by one or more of the methods. The positive rates for the detection of HPV DNA in condylomas by the different methods were 82% for Southern blot hybridization, 62% for in situ filter hybridization, and 72% for in situ tissue hybridization. Among the specimens from patients with cancer, HPV DNA was found in 83% (19 of 23) by one or more of the methods. Positive rates of 89 and 70%, respectively, were obtained for cancer lesions tested by the filter in situ and Southern blot hybridization methods; however, only 30% of those lesions were positive by the in situ tissue hybridization method. Thirteen percent of the control cervices were positive for HPV DNA by one or more of the assays. With respect to all disease categories, the methods had comparable sensitivities and specificities, except for the in situ tissue hybridization method, which revealed a specificity of 72% for condylomatous lesions and 30% for invasive cancer cells.
authors.affiliation: Molecular Virology and Immunology Program, McMaster University, Hamilton, Ontario, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Caussy
authors.forename: W
authors.initials: W
authors.lastname: Orr
authors.forename: A D
authors.initials: AD
authors.lastname: Daya
authors.forename: P
authors.initials: P
authors.lastname: Roth
authors.forename: W
authors.initials: W
authors.lastname: Reeves
authors.forename: W
authors.initials: W
authors.lastname: Rawls
mesh_terms: D002584:Cervix Uteri / Q000382:microbiology*; D003218:Condylomata Acuminata / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005833:Genital Neoplasms, Female / Q000382:microbiology*; D005834:Genital Neoplasms, Male / Q000382:microbiology*; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D002583:Uterine Cervical Neoplasms / Q000382:microbiology
chemical_list: D004279:DNA, Viral