AsnI: a novel class II restriction endonuclease from Arthrobacter sp., strain N-CM, recognizing 5'-AT/TAAT-3'.
A new class II restriction endonuclease, AsnI, with a novel sequence specificity was isolated from the Gram-positive eubacterium Arthrobacter species, strain N-CM. AsnI recognizes the unambiguously defined palindromic hexanucleotide (Formula: see text) consisting of A- and T-residues. The novel enzyme in the presence of Mg2+ cleaves specifically both strands as indicated by the arrows. The staggered cuts generate 5'-protruding ends with single-stranded 5'-TA-3' dinucleotide extensions. The novel enzyme may be a useful tool for cloning experiments by complementation of the few enzymes such as PstI and PvuI cutting only once in the Ampr-gene of plasmids pBR322 and pBR328.
Rexer
B U
BU
Boehringer Mannheim GmbH, Biochemical Research Center, Department of Molecular Biology, Penzberg, FRG.
Jarsch
M
M
Sagmeister
C
C
Glück
B
B
Berger
G
G
Kessler
C
C
D001173:Arthrobacter / Q000201:enzymology*; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D010957:Plasmids; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes; C056778:endodeoxyribonuclease AsnI; D015252:Deoxyribonucleases, Type II Site-Specific; D008274:Magnesium