Quantitative immunocytochemical assay for infectious avian retroviruses.
A simple and accurate immunocytochemical focus assay is described, whereby both transforming and non-transforming avian retroviruses can be enumerated. After virus infection of chick embryo fibroblasts, an agar overlay is applied; foci of infected cells (expression foci) are detected immunocytochemically after 5 to 7 days. The primary antibodies are monoclonal sera directed against either viral p19gag or pp60v-src. Detection of expression foci after transfection of cells with cloned viral DNA is also demonstrated.
Stoker
A W
AW
Division of Biology and Medicine, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Bissell
M J
MJ
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000254:growth & development; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D007150:Immunohistochemistry; D009052:Leukemia Virus, Murine / Q000254:growth & development; D012190:Retroviridae / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D014776:Virus Cultivation / Q000379:methods