Purification of brush border membrane vesicles from horse kidney cortex using Percoll.
A rapid method for preparation of brush border membrane vesicles from a large amount of horse kidney cortex is described. Self-orienting Percoll-gradient centrifugation minimized contamination by microsomal membranes. The characteristics of this preparation were checked by electron microscopy and measurement of L-alanine uptake.
Boudouard
M
M
Laboratoire de Biochimie, Faculté de Médecine, Nice, France.
Giudicelli
J
J
Sudaka
P
P
D000409:Alanine / Q000378:metabolism; D000818:Animals; D002499:Centrifugation, Density Gradient; D003102:Colloids; D006736:Horses / Q000378:metabolism*; D007672:Kidney Cortex / Q000648:ultrastructure*; D008566:Membranes / Q000032:analysis / Q000378:metabolism; D008861:Microsomes / Q000032:analysis; D008871:Microvilli / Q000032:analysis* / Q000648:ultrastructure; D011205:Povidone; D012822:Silicon Dioxide
D003102:Colloids; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; D000409:Alanine