Purinoceptors in the pulmonary circulation of the rat and their role in hypoxic vasoconstriction.
1. P2-purinoceptors have been characterized in the systemic circulation of a variety of species but little is known about their nature in the pulmonary vasculature. 2. In the isolated, blood perfused and ventilated lung of the rat the P2x selective analogues alpha,beta-methylene ATP(alpha,beta-meATP) (25 micrograms) and beta,gamma-methylene ATP (400 micrograms) caused a rise in pulmonary artery pressure (127 +/- 32% and 110 +/- 23% increase respectively, n = 6), demonstrating the existence of vasoconstrictor P2 receptors in the pulmonary circulation. 3. Repeated boluses of alpha,beta-meATP resulted in complete desensitization of the constrictor response to beta,gamma-meATP, but a small vasodilator response remained indicating unopposed stimulation of P2y receptors. The desensitization by alpha,beta-meATP had no effect on angiotensin II-induced vasoconstriction. 4. After desensitization of the vasoconstrictor P2 receptors with alpha, beta-meATP, hypoxic pulmonary vasoconstriction remained intact, indicating that these receptors are not involved in this response.
McCormack
D G
DG
Department of Thoracic Medicine, National Heart and Lung Institute, London.
Barnes
P J
PJ
Evans
T W
TW
D000255:Adenosine Triphosphate / Q000494:pharmacology / Q000031:analogs & derivatives; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D000860:Hypoxia / Q000503:physiopathology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000502:physiology; D010477:Perfusion; D011652:Pulmonary Circulation / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D011983:Receptors, Purinergic / Q000187:drug effects / Q000502:physiology*; D014661:Vasoconstriction / Q000187:drug effects
D011983:Receptors, Purinergic; D000804:Angiotensin II; C005147:5'-adenylyl (beta,gamma-methylene)diphosphonate; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate