The listeriolysin O gene: a chromosomal locus crucial for the virulence of Listeria monocytogenes.
In culture supernatants of a Tn 1545-induced non-hemolytic mutant of Listeria monocytogenes, by immunoblotting with an anti-serum raised against purified listeriolysin O, we have detected the presence of a truncated protein of 52,000D (the secreted listeriolysin O is 60,000D). The region of insertion of the transposon has been cloned and sequenced. The transposon had inserted in an open reading frame. The homologies detected between this ORF, streptolysin O and pneumolysin demonstrate the the transposon had indeed inserted in the listeriolysin O gene. As the non-hemolytic mutant was non-virulent, our work demonstrated that a genetic determinant essential for virulence is the listeriolysin O gene or its adjacent region.
Cossart
P
P
Département des Biotechnologies, Institut Pasteur, Paris.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005819:Genetic Markers; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins / Q000235:genetics*; D008089:Listeria monocytogenes / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D014774:Virulence
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D005819:Genetic Markers; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes