[Fatty-acid initiated auto-oxidation of hemoglobin].
The interaction of fatty acids (FA) with oxyhemoglobin (HbO2) was investigated. It was found that under effect of FA HbO2 is converted into the oxidized low-spin form, i. e., hemichrome. An increase in the level of reactive oxygen species was registered by the ESP method, using the spin traps, 1.2-dioxybenzo-3.5-disulphonate (tiron) and 5.5-dimethylpyrroline-1-oxide (DMPO). To investigate the nature of radical particles, the OH radical traps. thiourea and D-mannitol, as well as superoxide dismutase (SOD) and catalase were employed. The mechanism of interaction of HbO2 and FA is discussed; its feasible physiological role in the functioning of erythrocytes is postulated.
Akhrem
A A
AA
Andreiuk
G M
GM
Gurinovich
N A
NA
Kisel'
M A
MA
Kiselev
P A
PA
D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism*; D006461:Hemolysis; D007700:Kinetics; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D003574:Cytochrome c Group; D005227:Fatty Acids; D010108:Oxyhemoglobins; D013481:Superoxides
Initsiiruemoe zhirnymi kislotami avtookislenie gemoglobina.