Single-step procedure for the extraction and purification of leukotrienes B4, C4 and D4.
A rapid and simple method for the on-line concentration and high-performance liquid chromatographic (HPLC) purification of the leukotrienes in good yield from biological fluids is described. Readily available antisera are used in conjunction with this system to give a specific and sensitive assay for leukotrienes B4, C4 and D4 with sub-nanogram limits of detection. Tritium-labelled leukotrienes are used as internal standards, both to locate the leukotrienes post-HPLC and to accurately determine recoveries.
Richmond
R
R
Department of Clinical Pharmacology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Turner
N C
NC
Maltby
N
N
Heavey
D
D
Vial
J
J
Dollery
C T
CT
Taylor
G W
GW
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D006632:Histamine / Q000096:biosynthesis; D007118:Immunoassay; D007202:Indicators and Reagents; D007975:Leukotriene B4 / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D008168:Lung / Q000378:metabolism; D008297:Male; D013189:SRS-A / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D013189:SRS-A; D007975:Leukotriene B4; D006632:Histamine