title: Phosphatidylinositol 4,5-bisphosphate phosphodiesterase in higher plants.
abstract: A phospholipase C which hydrolyses phosphatidylinositol 4,5-bisphosphate to release inositol trisphosphate was detected in a sedimentable fraction from celery and from some other higher plants. The particulate enzyme also hydrolyses phosphatidylinositol, whereas the soluble phosphatidylinositol phosphodiesterase described previously [Irvine, Letcher & Dawson (1980) Biochem. J. 192, 279-283] acts only on phosphatidylinositol, and we were unable to detect activity of this soluble activity on phosphatidylinositol 4,5-bisphosphate. Activity of the particulate enzyme is markedly enhanced in the presence of deoxycholate, but not of other detergents; the particulate enzyme can also be solubilized by extraction with deoxycholate.
authors.affiliation: Department of Biochemistry, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
authors.forename: W C
authors.initials: WC
authors.lastname: McMurray
authors.forename: R F
authors.initials: RF
authors.lastname: Irvine
mesh_terms: D002118:Calcium / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D006868:Hydrolysis; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000378:metabolism; D008055:Lipids / Q000494:pharmacology; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D010944:Plants / Q000187:drug effects / Q000201:enzymology*
chemical_list: D007295:Inositol Phosphates; D008055:Lipids; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D003840:Deoxycholic Acid; D015544:Inositol 1,4,5-Trisphosphate; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D002118:Calcium