Inhibition by nedocromil sodium of early and late phase bronchoconstriction and airway cellular infiltration provoked by ovalbumin inhalation in conscious sensitized guinea-pigs.
Bronchial challenge of ovalbumin-sensitized conscious guinea-pigs induced a triphasic reduction in specific airways conductance (sGaw) with maximal reductions being observed at 2, 17 and 72 h accompanied by infiltration of the airways with neutrophils at 17 h and eosinophils at 17 and 72 h. Nedocromil sodium (10 mg ml-1) inhaled before challenge blocked the 2 and 17 h sGaw response and the neutrophil influx. When inhaled 6 h after challenge, nedocromil inhibited the 17 h sGaw response but not neutrophil influx, suggesting that these are unrelated. The sGaw response and eosinophil accumulation at 72 h was also inhibited by nedocromil given at this time. Our findings correlate with the clinical effects of nedocromil, suggesting the guinea-pig model may be useful for investigating the mechanism of action of anti-asthmatic drugs.
Hutson
P A
PA
Immunopharmacology Group, Southampton General Hospital.
Holgate
S T
ST
Church
M K
MK
D000280:Administration, Inhalation; D000818:Animals; D001980:Bronchi / Q000187:drug effects*; D004804:Eosinophils / Q000187:drug effects; D006168:Guinea Pigs; D008297:Male; D017835:Nedocromil; D009504:Neutrophils / Q000187:drug effects; D010047:Ovalbumin / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology*; D007507:Therapeutic Irrigation
D011804:Quinolines; D017835:Nedocromil; D010047:Ovalbumin