Convenient vectors for cloning and sequencing EcoRI and HindIII fragments.
The polylinker regions of plasmid pUC and bacteriophage M13mp vectors have been specifically modified to provide alternative positions for cloning and reexcising EcoRI and HindIII fragments; the EcoRI and HindIII sites have been moved internal to BamHI and Bg/II sites. The location of EcoRI and HindIII sites in these HinEco vectors allows either selective linearization or excision of the cloned fragments at unique flanking sites.
Edenberg
H J
HJ
Hormone Research Institute, University of California, San Francisco 94143-0534.
Moss
L G
LG
Rutter
W J
WJ
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D001616:beta-Galactosidase / Q000235:genetics
D009841:Oligonucleotides; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D001616:beta-Galactosidase