High-performance liquid chromatographic determination of a new pyrazoloquinoline type benzodiazepine receptor antagonist and its hydroxy metabolite.
A rapid high-performance liquid chromatographic (HPLC) method for the determination of the novel benzodiazepine receptor antagonist 2-phenylpyrazolo[4,3-c]quinolin-3(5H)-one (CGS 8216) and its hydroxy metabolite is described. The method involves a solid-phase extraction with C18 disposable columns and quantification by HPLC with UV-detection. In plasma 20 ng/ml of the antagonist and 10 ng/ml of metabolite can be measured with a coefficient of variation of about 10%. The high sensitivity and selectivity of the assay makes it suitable for use in pharmacokinetic studies.
Jedrychowski
M
M
Human Pharmacology Institute, Ciba-Geigy GmbH, Tübingen, Fed. Rep. of Germany.
Schmidt
E K
EK
Bieck
P R
PR
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011720:Pyrazoles / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D011804:Quinolines / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D011963:Receptors, GABA-A / Q000187:drug effects*
D011720:Pyrazoles; D011804:Quinolines; D011963:Receptors, GABA-A; C056594:CGS 11361; C033830:2-phenylpyrazolo(4,3-c)quinolin-3(5H)-one