Integration of a vector containing rodent repetitive elements in the rat genome.
We have previously shown that integration of a polyoma vector containing rodent repetitive elements into rat cellular DNA is non-random (Wallenburg et al. J. Virol. 50: 678-683). Junctions between the polyoma vector and the host DNA occur in the repetitive sequences of the vector about ten times more frequently than would be expected if sequences from the vector were used randomly for integration. In this paper we looked at the host sequences involved in these junctions. Our analysis did not reveal any repetitive or specific sequences and we presume therefore that the repetitive sequences of the vector acted as hot spots for illegitimate recombination. We also analysed the integration mechanism and found that: First, even though the polyoma vector was transfected in the presence of carrier DNA, integration did not involve the formation of a transgenome. Second, in at least one of the clones analysed, integration resulted in deletion of host DNA sequences. Third, the host DNA displaced at the integration site was considerably longer than the integrated segment.
Wallenburg
J C
JC
Département de microbiologie, Faculté de médecine, Centre Hospitalier Universitaire, Sherbrooke, Québec, Canada.
Nepveu
A
A
Chartrand
P
P
D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant / Q000032:analysis; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000235:genetics*; D051381:Rats / Q000235:genetics*; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
D004274:DNA, Recombinant