title: Insulin-mediated H+ and NH+4 excretion in the urinary bladder of Bufo marinus.
abstract: This study was done to determine if insulin mediates H+ and NH+4 excretion in the urinary bladder of Bufo marinus. Acidosis was induced by gavaging with 10 ml of 120 mM NH4Cl 3X daily for 2 days. Hemibladders were mounted between Lucite chambers. Insulin (porcine) was added to the serosal solution of the experimental bladder (10(2) mU/ml). After a 15-min equilibration the flux was measured for 2 hr. H+ excretion was measured from change in pH of the mucosal fluid and the NH+4 measured colorimetrically. The excretion was normalized for weight of bladder and reported in units of nanomoles (100 mg bladder)-1(min)-1. Plasma insulin was determined by radioimmunoassay and glucose by the glucose oxidase method. In 14 control bladders H+ excretion was 8.75 +/- 1.28 and experimental was 16.35 +/- 2.50 (P less than 0.025), while NH+4 excretion in control bladder was 3.29 +/- 0.95 and experimental was 6.58 +/- 1.89 (P less than 0.01). This response was absent when the insulin was heat inactivated (P greater than 0.2 and P greater than 0.3 respectively). Plasma insulin-like levels in 10 normal toads was 0.57 +/- 0.16 ngm/ml and in acidotic toads 1.25 +/- 0.16 ng/ml (P less than 0.025). Plasma glucose levels in 10 normal toads were 22.0 +/- 3.5 mg/dl and in 12 acidotic toads 17.8 +/- 0.75 mg/dl (P less than 0.025). We conclude that plasma insulin is increased in acidosis and that insulin stimulates excretion of H+ and NH+4 in the toad urinary bladder.
authors.affiliation: Department of Physiology, Baylor College of Dentistry, Dallas, Texas 75246.
authors.forename: L W
authors.initials: LW
authors.lastname: Frazier
mesh_terms: D000450:Aldosterone / Q000494:pharmacology; D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002024:Bufo marinus; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D007700:Kinetics; D011522:Protons; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D011522:Protons; D000450:Aldosterone; D000641:Ammonia