Superoxide anion production by postsurgical macrophages.
In order to characterize further the metabolic activity of postsurgical macrophages, we examined their potential to produce superoxide anion (O2-) in response to phorbol myristate acetate. Rabbits underwent resection and reanastomosis of their ileum after which peritoneal exudative cells (PEC) were collected at various times after surgery. Macrophages were isolated from the PEC with a discontinuous Percoll gradient. Thereafter, O2- release by these cells in response to phorbol myristate acetate (PMA) was determined by cytochrome c reduction. Macrophages recovered on postsurgical Days 3-7 expressed three- to fourfold greater O2- release than resident (nonsurgical) macrophages (P less than 0.001). In a time-course study of O2- release, maximum release occurred at 6 hr postsurgery, dropped to half of peak levels by Day 1, increased to peak levels again on Day 4, and remained increased until Day 7. The ability of macrophages to release O2- than gradually decreased, reaching control levels by Day 13. These data suggest that resident peritoneal macrophages are rapidly primed to produce enhanced amounts of superoxide anion in response to appropriate stimuli after surgery, and that macrophages subsequently recruited into the peritoneal cavity as a result of surgical trauma may also be primed for enhanced O2- release as they differentiate into modulator macrophages for tissue repair.
Fukasawa
M
M
Department of Obstetrics and Gynecology, University of Southern California School of Medicine, Los Angeles 90007.
Bryant
S M
SM
diZerega
G S
GS
D000818:Animals; D005260:Female; D008264:Macrophages / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D011817:Rabbits; D013481:Superoxides / Q000378:metabolism*; D013514:Surgical Procedures, Operative; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014945:Wound Healing
D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate