An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a.
We describe the construction and utilization of a new mobilizable cosmid vector. Using this vector, mobilizable libraries of bacterial DNA can be efficiently made without a need for size fractionation of target DNA. The low stability of this vector in Pseudomonas fluorescens makes it useful in a rapid strategy, which is not dependent on plasmid incompatibility, for recombining transposon-induced mutations into the bacterial chromosome.
Jones
J D
JD
Advanced Genetic Sciences Inc., Oakland, CA 94608.
Gutterson
N
N
D003360:Cosmids; D004247:DNA / Q000032:analysis; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D005819:Genetic Markers; D007120:Immunochemistry; D009154:Mutation; D010957:Plasmids; D011551:Pseudomonas fluorescens / Q000235:genetics*
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D005819:Genetic Markers; D004247:DNA