Selenium as a sulfhydryl redox catalyst and survey of potential selenium-dependent enzymes.
The ability of selenium (Se) to act as a redox catalyst is an important factor in understanding the biological function of selenoproteins in addition to that of GSH peroxidase. Selenocystine at micromolar levels exhibited pseudothiotransferase activity by enhancing the reduction of 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) by thiols. In contrast, selenite inhibited the reduction of DTNB by thiols. Selenite was more catalytic than selenocystine in the reduction of cytochrome c by GSH, whereas GSH peroxidase was a weak catalyst. Tissues from Se-deficient and Se-supplemented rats were assayed for activities of GSH-thiotransferase, NADPH cytochrome c reductase, formaldehyde dehydrogenase, and a hypothesized GSH cytochrome c reductase. GSH-thiotransferase activity was significantly increased in the liver of Se-deficient rats. No appreciable activity of this enzyme was found in the kidney of rats from either dietary group. No enzymatic activity for cytochrome c reduction by GSH was detected in cytosols, mitochondria, or microsomes from liver and kidney of Se-deficient or Se-supplemented rats. Formaldehyde dehydrogenase was significantly higher in liver cytosols from Se-supplemented rats than from Se-deficient rats. The higher activity was not attributed to Se-containing proteins, but to an unknown small molecular-weight factor. This study did not support the hypothesis that physiological levels of Se may be involved in sulfhydryl-disulfide exchange reactions in vivo, or that selenium may enhance cytochrome c reduction by GSH in vivo.
Hu
M L
ML
Department of Food Science and Technology, University of California, Davis 95616.
Tappel
A L
AL
D000818:Animals; D003553:Cystine / Q000031:analogs & derivatives / Q000494:pharmacology; D003574:Cytochrome c Group / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004228:Dithionitrobenzoic Acid; D004798:Enzymes / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D016566:Organoselenium Compounds; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000494:pharmacology*; D012645:Selenomethionine / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000378:metabolism
D003574:Cytochrome c Group; D004798:Enzymes; D016566:Organoselenium Compounds; D013438:Sulfhydryl Compounds; C009226:selenocystine; D003553:Cystine; D012645:Selenomethionine; D004228:Dithionitrobenzoic Acid; D005978:Glutathione; D012643:Selenium