Comparison of expression of the endo-beta-1,3-1,4-glucanase gene from Bacillus subtilis in Saccharomyces cerevisiae from the CYC1 and ADH1 promoters.
The endo-beta-1,3-1,4-glucanase gene from B. subtilis was placed under yeast promoter control in a number of different yeast expression vectors. The hybrid plasmids were transformed into S. cerevisiae where they directed the synthesis of varying amounts of active enzyme. The presence of B. subtilis DNA sequences 5' to the initiation codon for the B. subtilis beta-glucanase gene reduced expression of the gene in yeast. A 1,000-fold increase in the yield of beta-glucanase was obtained using the ADH1 promoter compared with the CYC1 promoter.
Cantwell
B A
BA
Research Centre, Arthur Guiness Son & Co. Ltd., Dublin, Ireland.
Brazil
G
G
Murphy
N
N
McConnell
D J
DJ
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003574:Cytochrome c Group / Q000235:genetics*; D045304:Cytochromes c; D005796:Genes; D005798:Genes, Bacterial; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic
C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D000426:Alcohol Dehydrogenase; D006026:Glycoside Hydrolases; C028020:licheninase