title: Nerve growth factor transiently increases tetrahydrobiopterin and total biopterin contents of pheochromocytoma PC12h cells.
abstract: Nerve growth factor (NGF) is known to induce differentiation of pheochromocytoma into sympathetic neuron-like cells. Tetrahydrobiopterin (BPH4) and total biopterin (BP) levels in PC12h, a subclonal line of PC12, were transiently increased by NGF: the increase in BPH4 and BP reached the maximum (20-25 ng/mg protein = about 2-fold over the control level) at 24 h after the treatment was started. After 2-3 days, the BPH4 and BP levels decreased to the same level as in control cells. The NGF concentration which gave a half maximal BP increase by 24 h-treatment was around 1 ng/ml.
authors.affiliation: Department of Pedodontics, Josai Dental University, Saitama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Nakanishi
authors.forename: S
authors.initials: S
authors.lastname: Yamada
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D001708:Biopterins / Q000031:analogs & derivatives* / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000378:metabolism*; D051381:Rats
chemical_list: D009414:Nerve Growth Factors; D001708:Biopterins; D003994:Bucladesine; C003402:sapropterin