[Demonstration of the isoenzymes of creatine kinase and lactate dehydrogenase using cellulose acetate strip electrophoresis].
Simple and sensitive methods for electrophoretic separation of diagnostic useful CK- and LDH-isoenzymes on cellulose acetate strips are described. The CK-bands were visualized with a commercial CK reagent using the fluorescence of NADPH as an indicator. The optimized reagents for LDH-isoenzymes detection contain a tetrazolium salt for staining the bands. Quantitative scanning of pherograms is possible.
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Urbahn
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D003402:Creatine Kinase / Q000032:analysis*; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D011237:Predictive Value of Tests; D012016:Reference Values
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase
Darstellung der Isoenzyme der Creatinkinase und Lactatdehydrogenase mittels Celluloseacetatfolienelektrophorese.