title: Functional characterization of an androgen response element in the first intron of the C3(1) gene of prostatic binding protein.
abstract: We demonstrate that the 204 bp intronic gene fragment of C3(1), which has a specific in vitro affinity for the androgen receptor, is able to confer androgen responsiveness to a heterologous promoter. This characteristic is completely destroyed by a single G----T substitution, affecting a 5'-TGTTCT-3' element that closely resembles the consensus sequence of the glucocorticoid and progesterone response elements (GRE/PRE). In fact we could show that this androgen response element (ARE) also acts as a similarly weak GRE or PRE in T-47D cells.
authors.affiliation: Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Claessens
authors.forename: L
authors.initials: L
authors.lastname: Celis
authors.forename: B
authors.initials: B
authors.lastname: Peeters
authors.forename: W
authors.initials: W
authors.lastname: Heyns
authors.forename: G
authors.initials: G
authors.lastname: Verhoeven
authors.forename: W
authors.initials: W
authors.lastname: Rombauts
mesh_terms: D000727:Androgen-Binding Protein / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015723:Gene Library; D005796:Genes; D007438:Introns; D008297:Male; D010957:Plasmids; D011401:Promoter Regions, Genetic; D060789:Prostatein; D011485:Protein Binding; D051381:Rats; D011944:Receptors, Androgen / Q000378:metabolism*; D015183:Restriction Mapping; D060765:Secretoglobins; D014162:Transfection; D014598:Uteroglobin; D060788:Mammaglobin B
chemical_list: D000727:Androgen-Binding Protein; D015500:Chloramphenicol O-Acetyltransferase; D060789:Prostatein; D011944:Receptors, Androgen; D060765:Secretoglobins; D014598:Uteroglobin; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B