Regulation of fetal Na+/K+-ATPase in rat kidney by corticosteroids.
The enzymatic differentiation of various tissues is under hormonal control in the perinatal period. Since the regulation of Na+/K+-ATPase has not been explored prenatally, the aim of this study was to determine the corticosteroid sensitivity of sodium pump maturation in the fetal period. Na+/K+-ATPase activity was both measured in kidney homogenates of fetal rats and localized by in-situ histochemistry. Sodium pump activity was first quantifiable on day 18 of fetal development as 1.4 +/- 0.17 mumol Pi/h per mg protein, and was increased 3.4-times by day 22 of gestation. While the Na+/K+-ATPase activity was the most intense in cortical tubules at an earlier fetal age (18th and 19th day), the reaction product in the medullary tubules increased with fetal age, becoming highly intense on the 21st and 22nd day of gestation. From the 18th to 21st day of fetal development homogenate Na+/K+-ATPase activity increased as a function of chronologic age. While mineralocorticoids were without any effect on Na+/K+-ATPase activity, on the last day of the fetal development, the glucocorticoid dexamethasone proved to be successful in stimulating enzyme activity in corticosteroid-suppressed animals. According to our results, glucocorticoid hormones seem to be operating as an endogenous driving force for sodium pump maturation at the end of fetal development.
Dobrović-Jenik
D
D
Laboratory of Pharmacology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Yugoslavia.
Milković
S
S
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000315:Adrenalectomy; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D003900:Desoxycorticosterone / Q000494:pharmacology; D005314:Embryonic and Fetal Development; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000196:embryology* / Q000201:enzymology; D008797:Metyrapone / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D011916:Rats, Inbred F344; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000305:Adrenal Cortex Hormones; D003900:Desoxycorticosterone; D009597:4-Nitrophenylphosphatase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D008797:Metyrapone