Localization of transfer RNA genes on the physical map of Vibrio eltor phage e4 genome.
Transfer RNAs were isolated from uninfected and phage e4-infected Vibrio eltor Mak 757 cells. These tRNAs were then aminoacylated with 3H-labeled amino acids and hybridized to DNA isolated from phage e4. Significant hybridization was observed only with tRNA isolated from phage e4-infected cells. Restriction enzyme digestion of phage e4 DNA followed by Southern blot using [32P]tRNA from infected cells revealed that tRNA genes were contained in a 3.4-kb Kpnl fragment. The tRNA genes were located on the physical map of the phage genome 19 kb from one of the termini.
Chattopadhyay
S
S
Indian Institute of Chemical Biology, Jadavpur, Calcutta.
Ghosh
R K
RK
D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D012343:RNA, Transfer / Q000235:genetics*; D014733:Vibrio / Q000235:genetics*
D012343:RNA, Transfer; D004262:DNA Restriction Enzymes