Selection and characterization of interferon response mutants from mouse L929 fibroblast cells.
Interferon (IFN) response mutants were selected from mouse L929 fibroblast cells and their specific resistance to is-1, an IFN-sensitive mutant of mengovirus, was studied. The standard L cell subline used in our laboratory (G3), is resistant to is-1 infection after pretreatment with low levels of IFN. Two clonal sublines that support the growth of is-1 in the presence of IFN (AS-4 and TA-6) were isolated from it, and two revertant lines (AS-4R1 and TA-6R1) were subsequently selected from AS-4 and TA-6. The kinetics of is-1 growth in the presence of IFN were found to vary in each of these sublines. Specific resistance to is-1 cannot be accounted for by enhanced induction of IFN, ability to bind IFN, or increased 2'-5'-oligo(A)-dependent endonuclease activity. AS-4 and TA-6 appear to have arisen through loss of one or more whole chromosomes. The origin of TA-6R1 is unclear.
Sakuragi
A L
AL
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Simon
E H
EH
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002872:Chromosome Deletion; D004722:Endoribonucleases / Q000032:analysis; D007370:Interferon Type I / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects* / Q000502:physiology; D008574:Mengovirus / Q000502:physiology*; D051379:Mice; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D007370:Interferon Type I; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease