A cDNA clone expressing a rhoptry protein of Plasmodium falciparum.
Antibodies from immune humans were used to select a cDNA clone expressing an asexual blood stage antigen of Plasmodium falciparum. The expressed fused polypeptide was used as an affinity reagent to purify human antibodies specific for the corresponding parasite antigen. Western blotting and immunoelectronmicroscopy demonstrated that the antigen was a 105 kDa protein located in the rhoptries of merozoites. The cDNA encodes the carboxy terminus of the rhoptry antigen, a sequence rich both in charged and hydroxy amino acids.
Coppel
R L
RL
Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.
Bianco
A E
AE
Culvenor
J G
JG
Crewther
P E
PE
Brown
G V
GV
Anders
R F
RF
Kemp
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D001483:Base Sequence; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D005455:Fluorescent Antibody Technique; D005796:Genes; D006801:Humans; D007118:Immunoassay; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology / Q000648:ultrastructure
D000953:Antigens, Protozoan; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII