Development of a lymphocyte transformation assay for rotavirus in whole blood and breast milk.
A multivariate assay was developed for detecting lymphoproliferative responses to rotavirus in whole blood and breast milk. Detection of lymphocyte transformation in samples from healthy uninfected adults required an assay incorporating a large number of antigen and cell concentrations and several incubation periods before pulsing. In contrast, lymphoproliferation to rotavirus was observed over a wider range of these variables in blood collected from a kidney recipient nine days post-rotavirus infection. Lymphoproliferation to rotavirus was detected in 32.5% of breast milk samples tested. The magnitude of lymphoproliferation to rotavirus did not correlate with rotavirus-specific IgG ELISA antibody titres in sera or with rotavirus-specific IgA ELISA antibody titres in breast milk.
Totterdell
B M
BM
Department of Virology, St. Thomas' Campus of United Medical School, Guy's Hospital, London.
Patel
S
S
Banatvala
J E
JE
Chrystie
I L
IL
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D008895:Milk, Human / Q000032:analysis / Q000276:immunology*; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology*
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G