BcefI, a new type IIS restriction endonuclease.
A new Type IIS restriction endonuclease was identified, partially purified and characterized from a Bacillus cereus subsp. fluorescens strain. The enzyme recognizes the nonpalindromic sequence ACGGC and cleaves at a distance from it. The cleavage appears to occur with a +/- 1 basepair uncertainty. Thus the cleavage and recognition site is as shown below: ACGGC(N)11-13 TGCCG(N)12-14.
Venetianer
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Institute of Biochemistry, Biological Research Center, Szeged, Hungary.
Orosz
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D001409:Bacillus cereus / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid
D004262:DNA Restriction Enzymes; C039566:CGCG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific