Influence of hydroxyl radical scavengers on platelet function.
The influence of four hydroxil radical (OH.) scavengers on platelet function was investigated. OH. scavengers inhibited ADP, collagen, arachidonic acid, PAF-induced platelet aggregation, and platelet cyclooxygenase pathway activation, which was studied by evaluating platelet malondialdehyde and serum thromboxane A2 formation. The latter was not affected by superoxide dismutase, catalase, or metal ion chelants such as desferioxamine or DETAPAC. The detection of deoxyribose degradation by stimulated platelets suggested that platelets produce OH.. This study shows that activated platelets produce free radicals and that antioxidant agents such as OH. scavengers inhibit platelet function.
Violi
F
F
Istituto di I Clinica Medica, Università degli Studi di Roma La Sapienza, Italy.
Ghiselli
A
A
Iuliano
L
L
Alessandri
C
C
Cordova
C
C
Balsano
F
F
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects*; D002374:Catalase / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005026:Ethylene Glycols / Q000494:pharmacology; D005260:Female; D005609:Free Radicals; D006801:Humans; D006878:Hydroxides; D017665:Hydroxyl Radical; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008782:Methylurea Compounds / Q000494:pharmacology; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D010979:Platelet Function Tests; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood; D013482:Superoxide Dismutase / Q000494:pharmacology; D013890:Thiourea / Q000494:pharmacology; D013929:Thromboxane B2 / Q000097:blood
D002614:Chelating Agents; D005026:Ethylene Glycols; D005609:Free Radicals; D006878:Hydroxides; D008782:Methylurea Compounds; D010975:Platelet Aggregation Inhibitors; C004168:1,1,3,3-tetramethylurea; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D013929:Thromboxane B2; D002374:Catalase; D011451:Prostaglandin-Endoperoxide Synthases; D013482:Superoxide Dismutase; D013890:Thiourea; D004121:Dimethyl Sulfoxide