Decrease of high affinity ouabain binding in rat cerebellum and hypothalamus by thiamin deficiency.
The effect of dietary thiamin deficiency on high affinity [3H]ouabain binding was examined in different regions of rat brain. The binding activity in the cerebellum and hypothalamus was significantly lower in the thiamin-deficient group than in the pair-fed control and freely fed control groups. The decrease was due to a change in Bmax but not in Kd. The results suggest a possible involvement of (Na+,K+)-ATPase in neurological manifestations of thiamin deficiency.
Matsuda
T
T
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Iwata
H
H
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D010042:Ouabain / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013832:Thiamine Deficiency / Q000378:metabolism*
D011955:Receptors, Drug; C028996:cardiac glycoside receptors; D010042:Ouabain; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase