Herpes simplex virus-directed overreplication of chromosomal DNA physically linked to the simian virus 40 integration site of a transformed hamster cell line.
In the simian virus 40 (SV40)-transformed hamster cell line Elona herpes simplex virus (HSV) induces amplification of SV40 DNA sequences to high-molecular-weight head-to-tail concatemers indicating an extrachromosomal rolling circle replication. In order to enable investigations concerning intrachromosomal amplification of SV40 DNA sequences and flanking cellular sequences a genomic library of Elona DNA was constructed in phage lambda. Clones harboring cellular DNA adjacent to the SV40 integration site were isolated. Plasmid subclones devoid of SV40 DNA sequences were used as hybridization probes against total DNA from HSV-infected cells. Thus the amplification of both flanking cellular sequences was demonstrated, indicating a bidirectional replication mode.
Gerspach
R
R
Institut für Medizinische Mikrobiologie und Hygiene, Abteilung für Virologie, Universität Freiburg, Federal Republic of Germany.
Matz
B
B
D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D004274:DNA, Recombinant / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis*; D005784:Gene Amplification; D008647:Mesocricetus; D011995:Recombination, Genetic; D013539:Simian virus 40 / Q000235:genetics*; D018139:Simplexvirus / Q000502:physiology*; D014407:Tumor Cells, Cultured
D004274:DNA, Recombinant; D004279:DNA, Viral; D004247:DNA