Genetic studies on toxic shock syndrome toxin-1 (TSST-1) producing Staphylococcus aureus strains from Austria, the USA and West Germany.
DNA preparations obtained from 10 different toxic shock syndrome toxin-1 positive S. aureus strains from Austria, the USA and West Germany were examined in respect of their homology to the TSST-1 gene of pRN6101. Whole cell DNA was digested with HpaII or EcoRI, separated on agarose gels and transferred to nitrocellulose; furthermore the dotblot procedure was used. The radioactively labelled gene probe used was the 300 bp BamHI-HindII fragment of the TSST-1 gene region of pRN6101. All S. aureus strains had homology with this fragment. Homology was located on fragments of different molecular size. Therefore it is concluded that the TSST-1 DNA fragment has different locations in the chromosome of different S. aureus strains.
Dickgiesser
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Institut für Hygiene und Medizinische Mikrobiologie der Fakultät für Klinische Medizin, Mannheim der Universität Heidelberg.
D001317:Austria; D001427:Bacterial Toxins; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D005860:Germany, West; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D018089:Superantigens; D014481:United States
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; D004262:DNA Restriction Enzymes