title: Synthesis in Escherichia coli of the HTLV-I trans-acting protein p40x.
abstract: The pX gene of human T-cell leukemia virus type I (HTLV-I) encodes a protein that activates the expression of viral genes in trans. Plasmid constructs designed to express the pX gene under the control of either the temperature-inducible lambda PL promoter or the trp promoter were used to transform several Escherichia coli strains, including murein-lipoprotein and Ion mutant strains. Upon induction it was possible to detect the synthesis of a new polypeptide of approximately 40 kDa which reacted specifically with serum from an ATL patient.
authors.affiliation: Department of Molecular Genetics, Nippon Roche Research Center, Kanagawa, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Sánchez
authors.forename: M A
authors.initials: MA
authors.lastname: Richardson
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Furuichi
mesh_terms: D003001:Cloning, Molecular; D017977:Deltaretrovirus / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli; D005786:Gene Expression Regulation; D007163:Immunosorbent Techniques; D009687:Nuclear Proteins / Q000235:genetics*; D010957:Plasmids; D012191:Retroviridae Proteins / Q000235:genetics* / Q000276:immunology; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D009687:Nuclear Proteins; D012191:Retroviridae Proteins; D014157:Transcription Factors