Pleural carcinogenic potency of mineral fibers (asbestos, attapulgite) and their cytotoxicity on cultured cells.
The carcinogenicity of several samples of mineral fibers was tested following injection of 20 mg in the pleural cavity of noninbred Sprague-Dawley rats. Three samples of chrysotile asbestos (mean length: 3.2, 2.1, and 1.2 micron) induced mesotheliomas at a rate of 48, 52, and 19%, respectively. The first sample was acid leached prior to intrapleural injection; in that group, the percentage of mesotheliomas was reduced to 25%. Treatment with amosite and crocidolite resulted in the occurrence of 57 and 56% of mesotheliomas. Acid-treatment of amphiboles did not significantly modify the percentage of mesotheliomas. When the Stanton's fiber dimensions were taken into consideration to correlate with mesothelioma incidence, the observed number of mesotheliomas in the chrysotile-treated animals was much lower than that expected, suggesting that other fiber parameters (chemistry, physicochemistry) play a role in the carcinogenicity. Attapulgite fibers (mean length: 0.77 micron) did not induce tumor, and the mean survival time was of the same order as that observed in the control groups. The injection of quartz resulted in no mesothelioma but did result in 6 malignant histiocytic lymphomas (17%) and 2 malignant schwannomas (6%). In vitro experiments did not show strong correlation between cytotoxicity and the carcinogenic potency of these minerals, but the qualitative cellular responses might give some indications on the fiber's potency. In addition, the in vitro effects of the fibers seem to be modulated by their size.
Jaurand
M C
MC
INSERM U 139, Centre Hospitalier Universitaire (CHU) Henri Mondor, Créteil, France.
Fleury
J
J
Monchaux
G
G
Nebut
M
M
Bignon
J
J
D000818:Animals; D001194:Asbestos; D017632:Asbestos, Serpentine; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D008264:Macrophages / Q000378:metabolism; D008274:Magnesium; D017616:Magnesium Compounds; D008297:Male; D008654:Mesothelioma / Q000209:etiology; D010316:Particle Size; D010997:Pleural Neoplasms / Q000209:etiology*; D011650:Pulmonary Alveoli / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012825:Silicon; D017655:Silicon Compounds
D017632:Asbestos, Serpentine; D017616:Magnesium Compounds; D017655:Silicon Compounds; D001194:Asbestos; D008274:Magnesium; C026325:attapulgite; D012825:Silicon