Effect of calcium ionophore A23187 and of leukotrienes B4 and C4 on the adherence of human mononuclear leukocytes.
The adherence changes induced by the ionophore A23187 and by the leukotrienes, respectively, were investigated in 10 healthy persons. The calcium ionophore A23187 in concentrations of 200 nM, 1 microM and 5 microM induced inhibition of mononuclear leukocyte adherence in a concentration-dependent manner, the maximal adherence inhibition being proved with 5 microM ionophore. The leukotrienes did not significantly alter leukocyte adherence in the test system used. The results of this study suggest that the calcium ionophore A23187-induced leukocyte adherence inhibition may serve as a pharmacological model of the leukocyte adherence inhibition phenomenon, thus providing a useful tool for the investigation of the processes associated with some cellular membrane functions.
Mayer
M
M
Palacký University, Department of Biochemistry, Olomouc, Czechoslovakia.
D000001:Calcimycin / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D007975:Leukotriene B4 / Q000494:pharmacology*; D013189:SRS-A / Q000494:pharmacology*
D013189:SRS-A; D007975:Leukotriene B4; D000001:Calcimycin