[Human monoclonal anti-cytomegalovirus antibodies].
Specific human monoclonal anti-CMV antibodies have been isolated and characterized. The first patient had a chronic T cell lymphocytic leukaemia and a subclinical CMV infection developed at the same time as a monoclonal peak of kappa IgG3. The purified F (ab')2 fragment of the IgG3 had an intense anti-CMV activity. A second monoclonal antibody, also a kappa IgG3, was isolated in a non-immunodepressed patient with a primary CMV infection (chronic pyrexia and hepatitis). The immunotransfer showed that the anti-CMV IgM and IgG of the patient's serum reacted particularly with the p51 protein of virus capsid. The monoclonal IgG3 was specific for the same p51. The third monoclonal anti-CMV Ig studied was synthesized in vitro by the B lymphocytes of a renal transplant patient immortalized by the Epstein-Barr virus. This kappa IgM produced continuously reacted strongly with the nucleus of the cells infected by the CMV. Human monoclonal anti-CMV antibodies could be used for the early detection of viral antigens by immunofluorescence and might also be used to treat severe cases of CMV infection.
Seigneurin
J M
JM
Laboratoire de Virologie, Centre Hospitalier Universitaire, Grenoble.
Desgranges
C
C
D000293:Adolescent; D000368:Aged; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000914:Antibodies, Viral / Q000302:isolation & purification*; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D016030:Kidney Transplantation; D007945:Leukemia, Lymphoid / Q000276:immunology; D008297:Male; D008875:Middle Aged; D014181:Transplantation Immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains
Anticorps monoclonaux humains anti-cytomégalovirus.