title: Carrier-free 8-azidoadenosine 5'-[gamma-32P]triphosphate.
abstract: We found 8-azidoadenosine 5'-diphosphate to be a phosphoryl acceptor in the enzymatic conversion of 1,3-diphosphoglyceric acid to 3-phosphoglycerate. This has allowed us to synthesize in a single-step procedure carrier-free 8-azidoadenosine 5'-[gamma-32P]triphosphate, requiring no further purification of the end product. The synthesized 8-azidoadenosine 5'-[gamma-32P]triphosphate has been characterized and shown to meet all the criteria for a specific photoreactive ATP analogue.
authors.affiliation: UCT-CSIR Research Centre for Molecular Biology, Department of Biochemistry, University of Cape Town, Republic of South Africa.
authors.forename: G P
authors.initials: GP
authors.lastname: Sabbatini
authors.forename: C
authors.initials: C
authors.lastname: von Holt
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D000345:Affinity Labels / Q000138:chemical synthesis / Q000378:metabolism; D001386:Azides / Q000138:chemical synthesis* / Q000378:metabolism; D007700:Kinetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D000345:Affinity Labels; D001386:Azides; C017040:8-azidoadenosine 5'-triphosphate; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase