Genetic and phenotypic characterization of mutants in four essential genes that map to the left half of HSV-1 UL DNA.
Several HSV-1 proteins including the major capsid protein (VP5), two minor capsid proteins (VP11-12 and VP18.8), the alkaline nuclease and glycoprotein gH have been reported to be encoded by the left-most one-third of HSV-1 UL DNA. In this paper, we present physical mapping data and phenotypic analysis of six ts mutants whose mutations lie within this region and which collectively represent four functional complementation groups (1-6, 1-7, 1-10, and 1-26). In this study, mutants in complementation group 1-10 were found to be defective in the synthesis of viral DNA, late viral polypeptides, and the formation of mature capsid-like structures--properties characteristic of other ts mutants defective in functions required for viral DNA synthesis. Two DNA-positive mutants in complementation group 1-7 fail to induce capsid formation and probably possess mutations in coding sequences for VP5. Mutants in two other complementation groups (1-6 and 1-26) synthesize significant levels of viral DNA, late polypeptides, and capsids. The functions of the gene products represented by these mutants remain to be determined.
Weller
S K
SK
Department of Microbiology, University of Connecticut Health Center, Farmington 06032.
Carmichael
E P
EP
Aschman
D P
DP
Goldstein
D J
DJ
Schaffer
P A
PA
D000818:Animals; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D005816:Genetic Complementation Test; D006801:Humans; D008854:Microscopy, Electron; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis
D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes