Topography, purification and characterization of thyroidal 5'-nucleotidase.
1. Subcellular studies of bovine thyroid indicate that 5'-nucleotidase is predominantly associated with plasma membranes, although a considerable part of this ectoenzyme is also found internalized. 2. The enzyme displaying the features of a glycoprotein has been purified 1400 times by detergent solubilization and two subsequent affinity chromatographic steps. 3. Thyroidal 5'-nucleotidase can be classified as an unspecific metallo-dependent 5'-ribonucleotide phosphohydrolase. The native enzyme exists as a dimer (MW 150 kDalton), composed of two similar or identical subunits.
Peeters
C
C
RUCA-Laboratory for Human Biochemistry, University of Antwerp, Belgium.
de Wolf
M
M
Van Dessel
G
G
Lagrou
A
A
Hilderson
H
H
Dierick
W
W
D015720:5'-Nucleotidase; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009708:Nucleotidases / Q000302:isolation & purification* / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D013961:Thyroid Gland / Q000201:enzymology*
D009708:Nucleotidases; D015720:5'-Nucleotidase