Stimulatory effect of transferrin on the proliferation of embryonic mouse molar pre-odontoblasts and pre-ameloblasts in organ culture.
The effects of transferrin on the proliferation kinetics of these cells from day-14 lower first molars, cultured for 2-6 days in a chemically defined medium supplemented with 5 and 50 micrograms/ml of human diferric transferrin, were studied. Transferrin stimulated the mitotic and [3H]-thymidine labelling indices. These data were correlated with immunolocalization of the transferrin receptor using indirect immunofluorescence and specific monoclonal antibodies. The presence of specific transferrin receptors in pre-odontoblasts and pre-ameloblasts, and in ameloblasts of older teeth (day-18 to day-21), was also assessed by indirect immunofluorescence and binding experiments using iodinated transferrin.
Cam
Y
Y
Institut de Biologie Médicale, INSERM CJF No. 88-08, Faculté de Médecine, Strasbourg, France.
Boukari
A
A
Ruch
J V
JV
D000565:Ameloblasts / Q000187:drug effects; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D008963:Molar; D009804:Odontoblasts / Q000187:drug effects; D009805:Odontogenesis / Q000187:drug effects*; D009924:Organ Culture Techniques; D013268:Stimulation, Chemical; D014070:Tooth / Q000196:embryology*; D014168:Transferrin / Q000494:pharmacology*
D014168:Transferrin