A new set of lacZ fusion vectors, pUCPlac, for studying gene expression in Drosophila by P-mediated transformation.
A new vector, pUCPlac, was generated by introducing a truncated lacZ structural gene into pUChsneo [Steller and Pirrotta, EMBO J. 4 (1985) 167-171]. In front of this gene a new polylinker was added which will allow the in-frame fusion of sequences containing cis-acting regulatory elements plus translation start site of any given gene. After P-mediated germ-line transformation in Drosophila the action of these regulatory elements can be conveniently monitored by histochemical staining for beta-galactosidase activity. Correct cloning can be easily ascertained by supercoil plasmid sequencing of the constructs using commercially available primers.
Molsberger
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G
Institut für Genetik, Universität Düsseldorf, F.R.G.
Schäfer
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U
Schäfer
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D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis; D001483:Base Sequence; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D001616:beta-Galactosidase / Q000096:biosynthesis
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase