A clinical isolate of transposon Tn5 expressing streptomycin resistance in Escherichia coli.
The central region of transposon Tn5 carries three antibiotic resistance markers: neo, ble, and str. The str gene codes for a phosphotransferase that inactivates streptomycin. This activity is phenotypically expressed in several gram-negative bacteria but not in Escherichia coli. We identified a Tn5 variant in E. coli clinical isolates that express streptomycin resistance. This transposon carries a 6-base-pair deletion within the str gene, near the 3' end. The same kind of mutation had been previously obtained experimentally from Tn5.
Genilloud
O
O
Unidad de Genética Molecular, Hospital Ramón y Cajal, Madrid, Spain.
Blázquez
J
J
Mazodier
P
P
Moreno
F
F
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology; D007612:Kanamycin / Q000494:pharmacology; D007613:Kanamycin Resistance; D010957:Plasmids; D013307:Streptomycin / Q000494:pharmacology*
D004251:DNA Transposable Elements; D007612:Kanamycin; D004262:DNA Restriction Enzymes; D013307:Streptomycin