title: An archaebacterial RNA polymerase binding site and transcription initiation of the hisA gene in Methanococcus vannielii.
abstract: Transcription initiation of the hisA gene in vivo in the archaebacterium Methanococcus vannielii, as determined by nuclease S1 and primer extension analyses, occurs 73 base pairs (bp) upstream of the translation initiation site. Binding of M. vannielii RNA polymerase protects 43 bp of DNA, from 35 bp upstream (-35) to 8 bp downstream (+8) of the hisA mRNA initiation site, from digestion by DNase I and exonuclease III. An A + T rich region, with a sequence which conforms to the consensus sequence for promoters of stable RNA-encoding genes in methanogens, is found at the same location (-25) upstream of the polypeptide-encoding hisA gene. It appears therefore that a TATA-like sequence is also an element of promoters which direct transcription of polypeptide-encoding genes in this archaebacterium.
authors.affiliation: Molecular, Cellular and Developmental Biology Program, Ohio State University, Columbus 43210.
authors.forename: J W
authors.initials: JW
authors.lastname: Brown
authors.forename: M
authors.initials: M
authors.lastname: Thomm
authors.forename: G S
authors.initials: GS
authors.lastname: Beckler
authors.forename: G
authors.initials: G
authors.lastname: Frey
authors.forename: K O
authors.initials: KO
authors.lastname: Stetter
authors.forename: J N
authors.initials: JN
authors.lastname: Reeve
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D004720:Endonucleases; D019605:Euryarchaeota / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D012333:RNA, Messenger; D012321:DNA-Directed RNA Polymerases; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases