Cloning of mRNA sequence coding for sex-specific storage protein of Bombyx mori.
A major plasma protein, referred to as SP 1, exhibits sex- and stage-specific expression during the larval development of the silkworm, Bombyx mori. We have isolated a plasmid clone bearing a part of mRNA sequence coding for SP 1 and quantitated the amount of SP 1 mRNA by means of RNA blot hybridization using the cDNA probe. The developmental change in the amount of SP 1 mRNA in the fat body closely reflected that of the hemolymph concentration of SP 1, indicating that the biosynthesis of SP 1 is regulated in a sex- and developmental stage-specific fashion at the level of mRNA.
Izumi
S
S
Department of Biology, Tokyo Metropolitan University, Japan.
Sakurai
H
H
Fujii
T
T
Ikeda
W
W
Tomino
S
S
D000273:Adipose Tissue / Q000502:physiology; D000367:Age Factors; D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D007301:Insect Hormones / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012737:Sex Factors
D001798:Blood Proteins; D007301:Insect Hormones; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes