Endocytosis of the vasopressin receptor by anterior pituitary cells is increased by corticotropin-releasing factor (CRF).
Endocytosis of certain receptors such as the transferrin receptor and the EGF-receptor appears to be influenced by second messengers. If second messengers are involved in modulation of endocytosis, not only endocytosis of the stimulated receptor itself but also of receptors for other ligands on the cell surface may be influenced by receptor occupancy. Corticotropin-releasing factor (CRF) and vasopressin act synergistically on secretion of ACTH from the anterior pituitary. The results presented here demonstrate that CRF increases retrieval of the vasopressin receptor in anterior pituitary cells in primary culture without influencing the surface binding of vasopressin. This is not a function of an increased membrane turnover since endocytosis of the transferrin receptor is not influenced by CRF.
Mogensen
N
N
Protein Laboratory, Copenhagen, Denmark.
Saermark
T
T
Vilhardt
H
H
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011945:Receptors, Angiotensin / Q000378:metabolism*; D017483:Receptors, Vasopressin; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D003994:Bucladesine; D003346:Corticotropin-Releasing Hormone; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate