Suppression of experimental autoimmune uveitis in guinea-pigs by inhibition of phospholipase A2.
Retinal S-antigen mixed with complete Freund's adjuvant was used to induce experimental autoimmune uveitis (EAU) in guinea-pigs. Guinea-pigs receiving no treatment, was compared with test animals which received topically and systemically administered KLM-583B, a phospholipase A2 (PLA2) inhibitor, or subcutaneous (sub. cut) and topical corticosteroid treatment, as well as a test group which received cyclosporin A suc. cut. The best clinical suppression of EAU was obtained in the group treated suc. cut with KLM-538B. Steroids also suppressed the inflammation in the eyes but was not as effective as KLM-583B or cyclosporine A. PLA 2 activity in the aqueous humour and the myeloperoxidase (MPO) levels measured from iris-ciliary body were significantly lower in the groups treated suc. cut. with KLM-583B or cyclosporin A. Guinea-pigs treated suc. cut. with KLM-583B and cyclosporin A had the lowest antiserum titres to retinal S-antigen.
Mahlberg
K
K
Department of Ophthalmology, Helsinki University, Central Hospital, Finland.
Uusitalo
R
R
Uusitalo
H
H
Palkama
A
A
Tallberg
T
T
D000818:Animals; D000941:Antigens / Q000032:analysis; D001082:Aqueous Humor / Q000378:metabolism; D019393:Arrestin; D001327:Autoimmune Diseases / Q000188:drug therapy* / Q000276:immunology; D002924:Ciliary Body / Q000201:enzymology; D003524:Cyclosporins / Q000627:therapeutic use; D003907:Dexamethasone / Q000627:therapeutic use; D005136:Eye Proteins / Q000032:analysis / Q000378:metabolism; D006168:Guinea Pigs; D007498:Iris / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors* / Q000627:therapeutic use; D054467:Phospholipases A2; D014605:Uveitis / Q000188:drug therapy* / Q000276:immunology
D000941:Antigens; D019393:Arrestin; D003524:Cyclosporins; D005136:Eye Proteins; C055939:KLM 583B; D003907:Dexamethasone; D009195:Peroxidase; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2