The structure of anchorin CII, a collagen binding protein isolated from chondrocyte membrane.
cDNA clones for anchorin CII (Mr = 34,000), a collagen-binding protein, were isolated from a lambda gt 11 cDNA library prepared from chick cartilage mRNA. Several overlapping clones were characterized which gave rise to an open reading frame coding for 329 residues and a 3'-untranslated segment of 500 base pairs. The clones were identified as coding for anchorin by hybrid select translation analysis and by comparing the deduced amino acid sequence with the sequence of 10 tryptic peptides of the protein. A hydrophobic domain of 25 residues interrupted with 3 polar residues was identified with the carboxyl-terminal portion. There was no evidence for an aminoterminal signal peptide. Northern analysis revealed that the 5' probe hybridizes to a single 1.7-kilobases (kb) mRNA species, whereas the 3' probe hybridizes to two mRNA species of 1.7 kb and 5 kb, which are present in many cells including chondrocytes, crop cells, and fibroblasts. The level of anchorin mRNA in chick embryo fibroblasts was increased by infection with Rous sarcoma virus.
Pilar Fernandez
M
M
Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, Bethesda, Maryland 20892.
Selmin
O
O
Martin
G R
GR
Yamada
Y
Y
Pfäffle
M
M
Deutzmann
R
R
Mollenhauer
J
J
von der Mark
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins; D002356:Cartilage / Q000032:analysis; D002645:Chickens; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification; D004274:DNA, Recombinant; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000235:genetics; D038981:Receptors, Collagen; D014357:Trypsin
D002352:Carrier Proteins; D004274:DNA, Recombinant; D010446:Peptide Fragments; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D003094:Collagen; D004247:DNA; D014357:Trypsin