Cointegrate formation by IS50 requires multiple donor molecules.
The insertion sequence, IS50R, promotes cointegrate formation between a lambda::IS50R phage and the chromosome of Escherichia coli strain C. We show that formation of cointegrates mediated by IS50R between the non-replicating phage genome and the bacterial chromosome requires multiple donor molecules and depends on homologous recombination functions. We conclude that the two copies of IS50 present in the cointegrate originate in two different molecules. Thus, the existence of the cointegrate structure cannot be used as evidence for replication of IS50 sequences during IS50 transposition.
Lichens-Park
A
A
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Syvanen
M
M
D010582:Bacteriophage lambda / Q000235:genetics*; D004261:DNA Replication; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D011938:Rec A Recombinases / Q000378:metabolism; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D011938:Rec A Recombinases