Restriction endonuclease analysis of DNA from Chlamydia trachomatis biovars.
DNA from a total of 60 Chlamydia trachomatis isolates was examined by restriction endonuclease analysis. Strains from all established biovars and serovars were tested. There was great diversity between the mouse biovar and the lymphogranuloma venereum (LGV) and trachoma biovars. The LGV and trachoma biovar isolates generated similar fragment patterns; however, distinct fragments appeared to be unique to both biovars, thus allowing differentiation of these two major groups. In most cases, strains of the same serovar could be differentiated from one another when a battery of restriction enzymes was used. In addition, in some cases, certain restriction fragments appeared to be characteristic of strains from a particular geographical location. The DNA patterns generated by all C. trachomatis isolates differed greatly from the DNA patterns generated from the Chlamydia psittaci isolates tested, including TWAR, a human C. psittaci strain.
Peterson
E M
EM
Department of Pathology, University of California, Irvine 92717.
de la Maza
L M
LM
D002692:Chlamydia trachomatis / Q000145:classification / Q000235:genetics*; D002691:Chlamydophila psittaci / Q000145:classification / Q000235:genetics; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D010957:Plasmids; D012703:Serotyping
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI