title: Inactivation of [Leu5]-enkephalin in three isolated preparations: relative importance of aminopeptidase, endopeptidase-24.11 and peptidyl dipeptidase A.
abstract: Enkephalin had been shown to be almost exclusively hydrolyzed by three peptidases in the previous studies. In the present investigation, the relative importance of three enzymes in the inactivation of [Leu5]-enkephalin was examined in three isolated preparations. Results showed that amastatin-sensitive aminopeptidase played the greatest role in both guinea-pig ileum and rat vas deferens while it played the similar role to either phosphoramdidon-sensitive endopeptidase-24.11 or captopril-sensitive peptidyl dipeptidase A in mouse vas deferens.
authors.affiliation: Department of Pharmacology, School of Medicine, Tokai University, Isehara, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: K
authors.initials: K
authors.lastname: Aoki
authors.forename: M
authors.initials: M
authors.lastname: Kajiwara
authors.forename: K
authors.initials: K
authors.lastname: Ishii
authors.forename: Y
authors.initials: Y
authors.lastname: Kuno
authors.forename: T
authors.initials: T
authors.lastname: Hiranuma
authors.forename: T
authors.initials: T
authors.lastname: Matsumiya
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002216:Captopril / Q000494:pharmacology; D004743:Enkephalin, Leucine / Q000378:metabolism*; D006020:Glycopeptides / Q000494:pharmacology; D006168:Guinea Pigs; D006868:Hydrolysis; D007082:Ileum / Q000201:enzymology; D007700:Kinetics; D008297:Male; D008666:Metalloendopeptidases / Q000378:metabolism*; D051379:Mice; D015260:Neprilysin; D009842:Oligopeptides / Q000494:pharmacology; D010455:Peptides; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D014649:Vas Deferens / Q000201:enzymology
chemical_list: D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D009842:Oligopeptides; D010455:Peptides; D004743:Enkephalin, Leucine; C016977:amastatin; D002216:Captopril; D007703:Peptidyl-Dipeptidase A; D008666:Metalloendopeptidases; D015260:Neprilysin; C008890:phosphoramidon