Identification of progesterone and cortisol as immunoreactive plasma digitalis like factors in pregnancy.
Plasma values for immunoreactive digitalis like factors (DLF) in 3 women during the third trimester of pregnancy were 0.13, 0.13 and 0.40 ng digoxin equivalents/ml. Progesterone accounted for 39%, 35% and 19% of the total DLF in the three women respectively, and cortisol 4%, 18% and 7% based on HPLC analysis. Both progesterone and cortisol displaced [125I]-digoxin from digoxin antibody and [3H]-ouabain from hog brain Na,K-ATPase in a concentration-dependent manner, but only progesterone in the plasma concentrations found in these patients would appreciable influence Na,K-ATPase. HPLC separation of the plasma DLF from two of the women showed several peaks. Two of these were identified as progesterone and cortisol by retention time, immunoassay and by GC-mass spectrometry.
Longerich
L
L
General Hospital, Memorial University of Nfld., St. John's.
Brent
D A
DA
Johnson
R L
RL
Vasdev
S
S
Gault
M H
MH
D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D002298:Cardenolides; D004077:Digoxin; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D011247:Pregnancy / Q000097:blood*; D011374:Progesterone / Q000097:blood*; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D011374:Progesterone; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase; D006854:Hydrocortisone