Clustering of tRNA genes in Paracentrotus lividus mitochondrial DNA.
We have determined the base sequence of the restriction fragment Bam1-2 (3,593) of Paracentrotus lividus (sea urchin) mtDNA. This fragment contains, in addition to genes previously identified (part of the 12S rRNA, ND1 and part of the ND2 mRNA), a cluster of 15 tRNA genes located between the 12S and ND1 genes. Also to be found in the tRNA gene cluster, between the tRNA(Thr) and tRNA(Pro) genes, is a sequence of 134 bp which constitutes the only non-coding region of this DNA so far identified. The distinctive organization of the tRNA genes and the extreme size reduction of the non-coding region suggest the existence of unique mechanisms for the regulation of gene expression in this organism.
Cantatore
P
P
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Italy.
Roberti
M
M
Rainaldi
G
G
Saccone
C
C
Gadaleta
M N
MN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000235:genetics*; D012617:Sea Urchins / Q000235:genetics*
D004272:DNA, Mitochondrial; D012343:RNA, Transfer; D004262:DNA Restriction Enzymes