title: Isolation of a cDNA encoding the muscle-specific subunit of human phosphoglycerate mutase.
abstract: We have isolated a full-length cDNA specifying the muscle-specific subunit of human phosphoglycerate mutase (PGAM-M). The cDNA encodes a deduced protein 253 amino acids in length and contains an unusually short (37 nucleotides) 3'-untranslated region. The deduced human PGAM-M protein is clearly related to yeast PGAM and to human diphosphoglycerate mutase. Genomic Southern analysis using the PGAM-M cDNA as a probe implies the presence of a large PGAM gene family in the human genome, while Northern analysis demonstrates tissue-specific transcription of this isoenzyme gene.
authors.affiliation: H. Houston Merritt Clinical Research Center for Muscular Dystrophy and Related Disorders, Columbia University, College of Physicians & Surgeons, New York, New York 10032.
authors.forename: S
authors.initials: S
authors.lastname: Shanske
authors.forename: S
authors.initials: S
authors.lastname: Sakoda
authors.forename: M A
authors.initials: MA
authors.lastname: Hermodson
authors.forename: S
authors.initials: S
authors.lastname: DiMauro
authors.forename: E A
authors.initials: EA
authors.lastname: Schon
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D010736:Phosphoglycerate Mutase / Q000032:analysis / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*; D011487:Protein Conformation
chemical_list: D004247:DNA; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase