title: Regulation of the rabbit ileal brush-border Na+/H+ exchanger by an ATP-requiring Ca++/calmodulin-mediated process.
abstract: Brush-border vesicles purified from rabbit ileal villus cells were used to evaluate how Ca++/calmodulin (CaM) regulates the neutral linked NaCl absorptive process, part of which is a Na+/H+ exchanger. After freezing and thawing to allow incorporation of macromolecules into the vesicles, the effect of Ca++/CaM on brush-border Na+ uptake with an acid inside pH gradient, and on Na+/H+ exchange was determined. Freezing and thawing vesicles with 0.85 microM free Ca++ plus 5 microM exogenous CaM failed to alter Na+/H+ exchange as did the addition of exogenous ATP plus an ATP regenerating system, which was sufficient to elevate intravesicular ATP to 47 microM from a basal level of 0.4 microM. However, the combination of Ca++/CaM plus ATP inhibited Na+ uptake in the presence of an acid inside pH gradient and inhibited Na+/H+ exchange, while Na+ uptake in the absence of a pH gradient was not altered. This effect required a hydrolyzable form of ATP, and did not occur when the nonhydrolyzable ATP analogue, AMP-PNP, replaced ATP. Under the identical intravesicular conditions used for the transport studies, Ca++ (0.85 microM) plus exogenous CaM (5 microM), in the presence of magnesium plus ATP, increased phosphorylation of five brush-border peptides. These data are consistent with Ca++/CaM acting via phosphorylation to regulate the ileal brush-border Na+/H+ exchanger.
authors.affiliation: Tufts University School of Medicine, Boston, Massachusetts.
authors.forename: R P
authors.initials: RP
authors.lastname: Rood
authors.forename: E
authors.initials: E
authors.lastname: Emmer
authors.forename: J
authors.initials: J
authors.lastname: Wesolek
authors.forename: J
authors.initials: J
authors.lastname: McCullen
authors.forename: Z
authors.initials: Z
authors.lastname: Husain
authors.forename: M E
authors.initials: ME
authors.lastname: Cohen
authors.forename: R S
authors.initials: RS
authors.lastname: Braithwaite
authors.forename: H
authors.initials: H
authors.lastname: Murer
authors.forename: G W
authors.initials: GW
authors.lastname: Sharp
authors.forename: M
authors.initials: M
authors.lastname: Donowitz
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000502:physiology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000502:physiology*; D002147:Calmodulin / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism*; D005615:Freezing; D007082:Ileum / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D011817:Rabbits; D017923:Sodium-Hydrogen Exchangers
chemical_list: D002147:Calmodulin; D002352:Carrier Proteins; D010455:Peptides; D017923:Sodium-Hydrogen Exchangers; D000255:Adenosine Triphosphate; D002118:Calcium