title: In vitro activities of daptomycin (LY146032) and paldimycin (U-70,138F) against anaerobic gram-positive bacteria.
abstract: The in vitro activities of daptomycin (LY146032), paldimycin (U-70,138F), vancomycin, and penicillin G against 344 clinical isolates of anaerobic gram-positive bacteria were determined by an agar dilution method in calcium-supplemented (50 micrograms/ml) Wilkins-Chalgren medium, using an inoculum of 10(5) CFU. Daptomycin demonstrated excellent activity against a broad range of anaerobic gram-positive cocci and bacilli, including Peptostreptococcus, Eubacterium, Bifidobacterium, Actinomyces, Propionibacterium, and Lactobacillus species and Clostridium difficile. Highly resistant strains (MIC, greater than or equal to 64 micrograms/ml) were encountered sporadically from different genera, but these accounted for only 3% of all isolates tested. Vancomycin showed similar activity but was less active against Lactobacillus species and Peptostreptococcus prevotii. Paldimycin was inactive against most genera of anaerobic gram-positive bacteria. Overall, penicillin G remained the most broadly active agent against these isolates.
authors.affiliation: Department of Medicine, University of British Columbia, Vancouver, Canada.
authors.forename: A W
authors.initials: AW
authors.lastname: Chow
authors.forename: N
authors.initials: N
authors.lastname: Cheng
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D003470:Culture Media; D017576:Daptomycin; D004187:Disaccharides; D006020:Glycopeptides / Q000494:pharmacology*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010400:Penicillin G / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004187:Disaccharides; D006020:Glycopeptides; D010455:Peptides; D014640:Vancomycin; C050461:paldimycin; D017576:Daptomycin; D010400:Penicillin G; D000111:Acetylcysteine