title: Monohydroxyeicosatetraenoic acid and leukotriene production by the inflammatory cells of Xenopus laevis.
abstract: Ten frogs (Xenopus laevis) were injected with mixed bacteria to produce a septic peritonitis. Peritoneal inflammatory cells of eight animals were studied for monohydroxyeicosanoid and leukotriene production from exogenous arachidonic acid. Large amounts of 12-hydroxyeicosatetraenoic acid were produced; smaller amounts of 5- and 15-hydroxyeicosatetraenoic and leukotriene B4 were produced. Identifications were confirmed by retention times on HPLC, ultraviolet spectroscopy on all products, and gas chromatograph/mass spectrometry in the case of 12-hydroxyeicosatetraenoic acid.
authors.affiliation: Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
authors.forename: F A
authors.initials: FA
authors.lastname: Green
authors.forename: C A
authors.initials: CA
authors.lastname: Herman
authors.forename: R P
authors.initials: RP
authors.lastname: Herman
authors.forename: H E
authors.initials: HE
authors.lastname: Claesson
authors.forename: M
authors.initials: M
authors.lastname: Hamberg
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis / Q000096:biosynthesis*; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology; D010538:Peritonitis / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid