title: High-performance liquid chromatographic determination of a new pyrazoloquinoline type benzodiazepine receptor antagonist and its hydroxy metabolite.
abstract: A rapid high-performance liquid chromatographic (HPLC) method for the determination of the novel benzodiazepine receptor antagonist 2-phenylpyrazolo[4,3-c]quinolin-3(5H)-one (CGS 8216) and its hydroxy metabolite is described. The method involves a solid-phase extraction with C18 disposable columns and quantification by HPLC with UV-detection. In plasma 20 ng/ml of the antagonist and 10 ng/ml of metabolite can be measured with a coefficient of variation of about 10%. The high sensitivity and selectivity of the assay makes it suitable for use in pharmacokinetic studies.
authors.affiliation: Human Pharmacology Institute, Ciba-Geigy GmbH, Tübingen, Fed. Rep. of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Jedrychowski
authors.forename: E K
authors.initials: EK
authors.lastname: Schmidt
authors.forename: P R
authors.initials: PR
authors.lastname: Bieck
mesh_terms: D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011720:Pyrazoles / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D011804:Quinolines / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D011963:Receptors, GABA-A / Q000187:drug effects*
chemical_list: D011720:Pyrazoles; D011804:Quinolines; D011963:Receptors, GABA-A; C056594:CGS 11361; C033830:2-phenylpyrazolo(4,3-c)quinolin-3(5H)-one