title: Establishment of stable mouse myeloma cells constitutively secreting human tissue-type plasminogen activator.
abstract: Expression plasmids for human tissue-type plasminogen activator (t-pA) were introduced into mouse myeloma cells and stable cell lines constitutively secreting t-pA established by selection with mycophenolic acid. Expression of t-pA is driven either by the simian virus 40 early promoter or by immunoglobulin regulatory elements of either light or heavy chains of the mouse. The availability of myeloma cells secreting a heterologous protein is of importance for biotechnological applications, because large-scale fermentation of myeloma cells is well established.
authors.affiliation: Biochemical Research Center, Boehringer Mannheim GmbH, Tutzing, F.R.G.
authors.forename: U H
authors.initials: UH
authors.lastname: Weidle
authors.forename: P
authors.initials: P
authors.lastname: Buckel
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D006801:Humans; D051379:Mice; D010954:Plasmacytoma; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000032:analysis; D013539:Simian virus 40 / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D011994:Recombinant Proteins; D004247:DNA; D004262:DNA Restriction Enzymes; D010959:Tissue Plasminogen Activator