title: Induction of cytosolic aspartate aminotransferase by glucagon in primary cultured rat hepatocytes.
abstract: The activity and the mRNA content of cytosolic aspartate aminotransferase (EC 2.6.1.1) were examined in cultured rat hepatocytes. Addition of glucagon (1 x 10(-7) M) in the presence of dexamethasone (1 x 10(-7) M) caused about 2-fold increase in the activity and mRNA content. Dibutyryl cAMP (1 x 10(-4) M) could replace glucagon for this effect. Maximal induction of cytosolic aspartate aminotransferase mRNA was observed 8 h after their additions. Insulin (1 x 10(-7) M) did not inhibit the enzyme induction by glucagon or dibutyryl cAMP. These results suggest that the cytosolic aspartate aminotransferase gene is regulated by cAMP, and not by insulin.
authors.affiliation: Department of Pharmacology II, Osaka University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Horio
authors.forename: H
authors.initials: H
authors.lastname: Fukui
authors.forename: M
authors.initials: M
authors.lastname: Taketoshi
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Wada
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000096:biosynthesis*; D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007328:Insulin; D012333:RNA, Messenger; C016276:8-bromocyclic GMP; D000001:Calcimycin; D003994:Bucladesine; D005934:Glucagon; D001219:Aspartate Aminotransferases; D006152:Cyclic GMP; D013755:Tetradecanoylphorbol Acetate