title: Collagenase-like (CL) peptidase activity in synovial fluid from patients with rheumatoid arthritis.
abstract: We found the presence of collagenase-like (CL) peptidase in synovial fluid by a highly sensitive fluorescence assay using (succinyl-Gly-Pro-Leu-Gly-Pro)-4-methyl-coumaryl-7-amide (Suc-GPLGP-MCA) as a substrate. Suc-GPLGP-MCA is hydrolyzed at the Leu-Gly bond by CL-peptidase. The CL-peptidase activity in synovial fluid was significantly higher in patients with rheumatoid arthritis (RA) than in patients with osteoarthritis (OA) and in arthropathy-free controls. No significant difference in CL-peptidase activity in synovial fluid was found between patients with OA and arthropathy-free controls.
authors.affiliation: Department of Orthopedic Surgery, School of Medicine, Nagoya University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Hagihara
authors.forename: T
authors.initials: T
authors.lastname: Nagatsu
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: T
authors.initials: T
authors.lastname: Miura
mesh_terms: D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D003374:Coumarins / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D003012:Microbial Collagenase / Q000032:analysis*; D009842:Oligopeptides; D010003:Osteoarthritis / Q000201:enzymology; D013582:Synovial Fluid / Q000201:enzymology*
chemical_list: D003374:Coumarins; D009842:Oligopeptides; C023859:succinylglycyl-prolyl-leucyl-glycyl-prolyl-4-methylcoumaryl-7-amide; D003012:Microbial Collagenase