[Detection of the provirus of the bovine leukemia virus in bovine peripheral blood leukocytes by means of DNA hybridization].
The method allowing the detection of BLV proviral DNA in the peripheral blood leukocytes of cattle is reported. Cell DNA from leukocytes used without preliminary cultivation was dot-hydridized with 32P-labeled plasmid that included a fragment of BLV proviral DNA. In parallel, sera from the cattle under study were tested by immunodiffusion assay (ID). The results indicate that dot-hybridization assay is more sensitive as a diagnostic test than ID because it detects BLV infection in apparently normal cattle which was seronegative by ID.
Rassulin
Iu Iu
IuIu
Fedorova
N A
NA
Rechinskiĭ
V O
VO
Parfanovich
M I
MI
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D004247:DNA / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005779:Immunodiffusion / Q000662:veterinary; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007962:Leukocytes / Q000276:immunology / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011533:Proviruses / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012190:Retroviridae / Q000302:isolation & purification*
D000914:Antibodies, Viral; D004279:DNA, Viral; D004247:DNA
Obnaruzhenie provirusa virusa leĭkoza krupnogo rogatogo skota v leĭkotsitakh perifericheskoĭ krovi krupnogo rogatogo skota metodom DNK-gibridizatsii.