Effect of Sendai inducing virus in cytopathic effect inhibition assay of bovine leukocyte interferon.
Various methods for removal or inactivation of Sendai inducing virus from natural bovine leukocyte interferon (IFN) preparations were compared and the effects of Sendai virus in microtiter cytopathic effect (CPE) inhibition assay were investigated. Ultraviolet light and ultracentrifugation at either 108,000 X g or 145,000 X g for 2 h proved to be inadequate methods for complete removal of inducing virus from IFN preparations, as judged by the effects of residual virus in CPE inhibition assay. Optimal methods for inducer virus removal/inactivation were ultracentrifugation at 175,000 X g or dialysis against pH 2.0 buffer. Sendai virus itself was capable of inducing IFN production in assay cells at concentrations commonly found in virus-induced IFN preparations.
Jacobsen
K L
KL
Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
Abolhassani
M
M
Chitwood
S
S
D000818:Animals; D001681:Biological Assay / Q000379:methods; D002417:Cattle; D003588:Cytopathogenic Effect, Viral; D007370:Interferon Type I / Q000032:analysis* / Q000096:biosynthesis; D010222:Parainfluenza Virus 1, Human / Q000276:immunology* / Q000302:isolation & purification; D014461:Ultracentrifugation
D007370:Interferon Type I