Seed-transmissible expression of mammalian metallothionein in transgenic tobacco.
A binary plasmid was constructed to contain the mouse metallothionein c-DNA, the constitutive 35S promoter from cauliflower mosaic virus, the polyadenylation signal from the pea rbcS-E9 gene and several selectable markers. The plasmid was transferred to Agrobacterium tumefaciens and the leaf disc method was used to transform tobacco. Callus and shoots were regenerated in the presence of kanamycin and transformed plants were obtained. Southern, Northern and Western blot analysis demonstrated integration and expression of the metallothionein gene in transformed callus and transgenic plants. The gene is transmitted to and expressed in seed derived progeny as a dominant Mendelian trait.
Maiti
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Department of Agronomy, University of Kentucky, Lexington 40546-0091.
Hunt
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AG
Wagner
G J
GJ
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D009029:Mosaic Viruses / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012231:Rhizobium / Q000235:genetics; D012639:Seeds; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic
D008668:Metallothionein; D004262:DNA Restriction Enzymes