Single-strand-specific nuclease from rat liver endoplasmic reticulum: characterization and mode of action.
1. The characteristics and mode of action of a single-strand-specific nuclease isolated from rat liver endoplasmic reticulum are investigated with respect to its DNA and RNA substrates. 2. The RNase activity of the enzyme is slightly influenced by the presence of divalent cations but the DNase activity is enhanced by divalent cations particularly Mn2+. 3. Activity is partially inhibited by the presence of EGTA; this effect is reversed most efficiently by the addition of Mn2+. 4. The enzyme exhibits small pH dependence between pH 6-9 and maximum activity is observed at pH 7-7.5 for both DNase and RNase activities. 5. Sulfhydryl group reagents do not affect its action but histidyl group reagents exert a small but definite effect. 6. The enzyme degrades DNA and RNA endonucleolytically producing fragments which possess 3'-OH and 5'-phosphate termini. 7. Monomers are not produced even after prolonged degradation. 8. The end product of poly(U)degradation ranges between two and four building blocks but the DNA product is longer probably due to considerable percentage of secondary structure.
Kouidou
S A
SA
Department of Biological Chemistry, School of Medicine, Aristotelian University of Thessaloniki, Greece.
Triantos
A A
AA
Papageorgiou
G E
GE
Kavoukopoulos
E M
EM
Vavatsi
N A
NA
Trakatellis
A C
AC
D000818:Animals; D002413:Cations, Divalent; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004720:Endonucleases / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D051381:Rats
D002413:Cations, Divalent; D004533:Egtazic Acid; D004492:Edetic Acid; D004720:Endonucleases; C054500:single-strand-specific nuclease, endoplasmic reticulum