Chromosomal mapping of the pel and cel genes in Erwinia chrysanthemi strain B374.
Using the RP4::mini-Mu in vivo cloning technique, van Gijsegem et al. (1985) isolated several pel and cel genes of Erwinia chrysanthemi (Ech) B374 strain. We have localized these genes on the Ech chromosome by co-transfer mapping of MudI1734 insertion mutants and refined the map by co-transposition analysis. This analysis has enabled us to identify another cel gene.
Piecq
M
M
Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium.
van Gijsegem
F
F
Schoonejans
E
E
Toussaint
A
A
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000502:physiology*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004885:Erwinia / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005959:Glucosidases / Q000235:genetics; D009154:Mutation; D009710:Nucleotide Mapping; D011133:Polysaccharide-Lyases / Q000235:genetics
D004262:DNA Restriction Enzymes; D005959:Glucosidases; D011133:Polysaccharide-Lyases; C023911:pectate lyase