Murine glia cells in culture can be stimulated to generate reactive oxygen.
Induction of luminol-enhanced chemiluminescence (CL) indicative of reactive oxygen formation was studied in glia cell cultures from newborn mice. A burst of CL could be induced by phorbol myristate acetate, zymosan, and antibody-coated bovine red blood cells, whereas Sendai virus and several other agents known to induce CL in myeloid cells were ineffective. Sodium azide failed to inhibit CL, indicating a myeloperoxidase-independent mechanism of light emission. In parallel experiments we identified the cells binding antibody-coated erythrocytes as macrophages characterized by the reduction of nitroblue tetrazolium and phagocytosis of zymosan and latex particles. Brain macrophages may use reactive oxygen intermediates (ROI) as a mechanism of antimicrobial defence; and, on the other hand, ROI formed by these cells may contribute to immuno-pathology in the brain.
Sonderer
B
B
Institute of Virology, University of Zürich, Switzerland.
Wild
P
P
Wyler
R
R
Fontana
A
A
Peterhans
E
E
Schwyzer
M
M
D000166:Acridines / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000906:Antibodies / Q000276:immunology; D001386:Azides / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008163:Luminescent Measurements; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009457:Neuroglia / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D009195:Peroxidase / Q000037:antagonists & inhibitors; D010587:Phagocytosis; D019810:Sodium Azide; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D000166:Acridines; D000906:Antibodies; D001386:Azides; D007074:Immunoglobulin G; C033472:10,10'-dimethyl-9,9'-biacridinium; D009580:Nitroblue Tetrazolium; D015054:Zymosan; D019810:Sodium Azide; D009195:Peroxidase; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen