title: Response of human glioblastoma cells to recombinant interleukin-2.
abstract: We investigated the ability of glioma cells to respond to T cell-derived lymphokines. The growth of astrocytoma and mixed glioblastoma cell lines, as assessed by DNA synthesis, was inhibited in the presence of supernatants derived from mitogen-stimulated human T cells, an HTLV-II-transformed human T cell line, Mo, and human interleukin-2 (IL-2). The mixed glioblastoma cell line, 138-MG-C, was subjected to limiting dilution analysis, and two cell lines (5D7, 5C5) were derived which were homogeneous with respect to staining for galactocerebroside (GalC) (100%). These two GalC+ glioblastoma cell lines proliferated in the presence of high concentrations of recombinant human interleukin-2 (RIL-2). Additionally, these cell lines bear receptors for the IL-2 molecule as determined by immunofluorescent staining with various anti-IL-2 receptor antibodies.
authors.affiliation: Department of Neurology, UCLA School of Medicine 90024.
authors.forename: E N
authors.initials: EN
authors.lastname: Benveniste
authors.forename: H
authors.initials: H
authors.lastname: Tozawa
authors.forename: J C
authors.initials: JC
authors.lastname: Gasson
authors.forename: S
authors.initials: S
authors.lastname: Quan
authors.forename: D W
authors.initials: DW
authors.lastname: Golde
authors.forename: J E
authors.initials: JE
authors.lastname: Merrill
mesh_terms: D001932:Brain Neoplasms / Q000503:physiopathology*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D005909:Glioblastoma / Q000503:physiopathology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*
chemical_list: D007376:Interleukin-2; D004247:DNA