A cloned DNA probe identifies the sex of Schistosoma mansoni cercariae.
This report describes a method for the identification of the sex of Schistosoma mansoni cercariae using a cloned DNA probe which is female-specific. The probe is a 339 bp repeat; the sequence is presented. Cercariae from nine mono-miracidially infected snails were used in a double blind study. Mice were infected and the sex of adult worms observed. DNA was isolated from cercariae, digested with EcoRI, subjected to agarose gel electrophoresis, transferred to a matrix and hybridized with the cloned female-specific DNA probe. In all nine cases the sex of the cercariae as determined by DNA analysis agreed with the sex of the adult parasites.
Spotila
L D
LD
Department of Microbiology, State University of New York, Buffalo 14214.
Rekosh
D M
DM
Boucher
J M
JM
LoVerde
P T
PT
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D004311:Double-Blind Method; D005260:Female; D005796:Genes; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012550:Schistosoma mansoni / Q000235:genetics*; D012732:Sex Determination Analysis
D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI