Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes.
Detection of viral DNA in infected tissues is demonstrated by in situ nucleic acid hybridization employing bromodeoxyuridine (BrdUrd)-labeled probes. BrdUrd is used alternatively to biotinylated nucleotides in the preparation of DNA probes and, after hybridization, developed with a monoclonal anti-BrdUrd antibody and the alkaline phosphatase-anti-alkaline phosphatase technique. The sensitivity using both BrdUrd- and biotin-labeled probes is similar. Furthermore, the simultaneous detection of viral coinfections will be possible by means of this technique in conjunction with biotin-labeled DNA probes.
Niedobitek
G
G
Institute for Pathology, Freie Universität Berlin, West Germany.
Finn
T
T
Herbst
H
H
Bornhöft
G
G
Gerdes
J
J
Stein
H
H
D001973:Bromodeoxyuridine; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000473:pathology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008168:Lung / Q000382:microbiology* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D004279:DNA, Viral; D001973:Bromodeoxyuridine