[Molecular organization of glutamate-sensitive chemoexcitable membranes of nerve cells. Function of glutamate-binding proteins of the central nervous system when incorporated into liposomes].
The functioning of the glutamate-binding protein of rat brain cortex synaptic membranes was studied by its incorporation into liposomes. The optimal conditions for the receptor protein incorporation were established and the kinetics of 22Na+ and 86Rb+ incorporation into the liposomes in the presence of L-glutamate were analyzed. Modelling of the CNS glutamate receptor functions was found to be dependent on the lipid composition and amount of the incorporated membrane protein. The selective transport of 22Na+ into the liposomes was stimulated in the presence of 10(-4) M glutamate. Addition of monoclonal antibodies against glutamate-binding proteins blocked the incorporation of Na+ into the liposomes. The experimental results are suggestive of the nativity of the liposome-incorporated membrane protein, which is capable of binding glutamate and regulating selective transport of Na+. It was assumed that the glutamate receptor macromolecule represents an integral complex made up of several low molecular weight subunits of glucoprotein nature that form a selective ionic channel.
Besedin
V I
VI
Kuznetsov
A S
AS
Dambinova
S A
SA
D000818:Animals; D000906:Antibodies; D001692:Biological Transport; D002540:Cerebral Cortex / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006023:Glycoproteins / Q000502:physiology; D008081:Liposomes; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000276:immunology / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013570:Synaptic Membranes / Q000378:metabolism
D000906:Antibodies; D005971:Glutamates; D006023:Glycoproteins; D008081:Liposomes; D009419:Nerve Tissue Proteins; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D018698:Glutamic Acid; D012964:Sodium; D012413:Rubidium
Molekuliarnaia organizatsiia glutamatchuvstvitel'nykh khemovozbudimyx memran nerviyx kletok. Izuchenie funktsii glutamatsviazyvaiushchikh belkov tsentral'noĭ nervnoĭsistemy metodom vstraivaniia v liposomy.