title: Synthesis and overproduction of the 5A protein of insertion sequence IS5.
abstract: We have demonstrated both the synthesis and overproduction of the 5A protein encoded by the longest open reading frame of the bacterial insertion sequence IS5. Expression was obtained in vitro and in Escherichia coli maxicells from plasmids containing IS5 in either orientation, as well as in vitro from a restriction fragment containing exclusively IS5 DNA. When IS5 was cloned in the appropriate orientation downstream of a strong tac promoter, production of the 5A protein was increased to 10 to 20% of the total protein synthesized in vitro.
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: J M
authors.initials: JM
authors.lastname: Chernak
authors.forename: E J
authors.initials: EJ
authors.lastname: Schlaffer
authors.forename: H O
authors.initials: HO
authors.lastname: Smith
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D007700:Kinetics; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D002352:Carrier Proteins; D004251:DNA Transposable Elements