title: Suppression of monosodium urate crystal-induced acute inflammation by diets enriched with gamma-linolenic acid and eicosapentaenoic acid.
abstract: A subcutaneous air pouch formed in Sprague-Dawley rats was used to study the effect of diets enriched in gamma-linolenic acid (GLA) (in plant seed oil) and eicosapentaenoic acid (EPA) (in fish oil) on acute inflammation induced by monosodium urate crystals. The GLA-enriched diet suppressed significantly the cellular phase of inflammation (polymorphonuclear leukocyte accumulation, crystal phagocytosis, and lysosomal enzyme activity), but it had little effect on the fluid phase (exudate volume and protein concentration). In contrast, the EPA-enriched diet suppressed the fluid phase but not the cellular phase of inflammation. The findings indicate that the fluid and cellular phases of acute inflammation can be controlled independently. A combined diet of fish oil and plant seed oil (EPA-enriched and GLA-enriched) reduced both the cellular and fluid phases of inflammation. Thus, dietary provision of alternative substrates for oxidative metabolism (other than arachidonic acid) modifies monosodium urate crystal-induced acute inflammation.
authors.affiliation: Department of Medicine, University of Pennsylvania, Philadelphia.
authors.forename: G A
authors.initials: GA
authors.lastname: Tate
authors.forename: B F
authors.initials: BF
authors.lastname: Mandell
authors.forename: R A
authors.initials: RA
authors.lastname: Karmali
authors.forename: M
authors.initials: M
authors.lastname: Laposata
authors.forename: D G
authors.initials: DG
authors.lastname: Baker
authors.forename: H R
authors.initials: HR
authors.lastname: Schumacher
authors.forename: R B
authors.initials: RB
authors.lastname: Zurier
mesh_terms: D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000627:therapeutic use*; D015232:Dinoprostone / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000627:therapeutic use*; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism; D005228:Fatty Acids, Essential / Q000627:therapeutic use; D005260:Female; D005395:Fish Oils / Q000627:therapeutic use; D007249:Inflammation / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology; D008041:Linoleic Acids; D008042:Linolenic Acids / Q000627:therapeutic use*; D008297:Male; D031665:Oenothera biennis; D010587:Phagocytosis; D010938:Plant Oils; D051381:Rats; D011919:Rats, Inbred Strains; D014527:Uric Acid; D017965:gamma-Linolenic Acid
chemical_list: D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D005395:Fish Oils; D008041:Linoleic Acids; D008042:Linolenic Acids; D010938:Plant Oils; C033266:Menhaden oil; D014527:Uric Acid; C028498:evening primrose oil; D017965:gamma-Linolenic Acid; D015118:Eicosapentaenoic Acid; D015232:Dinoprostone