title: Production of active human carbonic anhydrase II in E. coli.
abstract: cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.
authors.affiliation: Department of Biochemistry, University of Umeå, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Forsman
authors.forename: G
authors.initials: G
authors.lastname: Behravan
authors.forename: A
authors.initials: A
authors.lastname: Osterman
authors.forename: B H
authors.initials: BH
authors.lastname: Jonsson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D004247:DNA; D004262:DNA Restriction Enzymes; D002256:Carbonic Anhydrases