Periodate-methylamine degradation of ribonucleotides in rat liver extracts in comparison to extracts of cultured cells.
Periodate-methylamine degradation of ribonucleotides, which enables separation of deoxyribonucleotides in cell extracts by high-performance liquid chromatography, was tested on the acid-soluble fraction of L1210 cells, Ehrlich ascites tumor (ELD) cells, liver tissue, and liver cell nuclei. It was shown that dTTP, dCTP, and dATP could be clearly separated in L1210 and ELD extracts. In samples from liver tissue, however, dTTP coeluted with another compound, the dCTP peak often eluted very close to another peak, and only dATP separated quite satisfactorily. Furthermore, extracts from liver cell nuclei, isolated in glycerol, were not directly susceptible to periodate oxidation. This problem can be avoided by use of a different procedure for the isolation of cell nuclei.
Andersson
M
M
Department of Zoophysiology, University of Lund, Sweden.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003854:Deoxyribonucleotides / Q000302:isolation & purification*; D007939:Leukemia L1210 / Q000032:analysis; D008099:Liver / Q000032:analysis; D008744:Methylamines; D010504:Periodic Acid; D051381:Rats; D012265:Ribonucleotides / Q000302:isolation & purification; D014407:Tumor Cells, Cultured / Q000032:analysis
D003854:Deoxyribonucleotides; D008744:Methylamines; D012265:Ribonucleotides; D010504:Periodic Acid; C009288:metaperiodate; C027451:methylamine