Somatostatin and alpha 2-adrenergic agonists selectively inhibit vasopressin-induced cyclic AMP accumulation in MDCK cells.
The effect of somatostatin and alpha 2-adrenergic agonists on cyclic AMP accumulation was examined in MDCK cells, grown in defined medium. These hormones inhibited vasopressin-induced cyclic AMP formation, without affecting either the basal or the glucagon- and prostaglandin E2-stimulated level. Pretreating the cells with pertussis toxin, or incubating them with MnCl2 at a low concentration reversed the effect of somatostatin and alpha 2-agonists. These results suggest that somatostatin and norepinephrine could selectively modulate the renal effect of vasopressin, via the inhibitory regulatory subunit (Ni) of adenylate cyclase.
Friedlander
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G
Amiel
C
C
D037361:Adenylate Cyclase Toxin; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D015232:Dinoprostone; D004285:Dogs; D005934:Glucagon / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D037342:Pertussis Toxin; D011458:Prostaglandins E / Q000494:pharmacology; D013004:Somatostatin / Q000494:pharmacology*; D014667:Vasopressins / Q000494:pharmacology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D000316:Adrenergic alpha-Agonists; D011458:Prostaglandins E; D010566:Virulence Factors, Bordetella; D014667:Vasopressins; D008345:Manganese; D013004:Somatostatin; D005934:Glucagon; D000242:Cyclic AMP; D037342:Pertussis Toxin; D015232:Dinoprostone