Proto-oncogene homologous sequences in the sea urchin genome.
Using probes specific for several oncogenes/proto-oncogenes we have performed gel blot hybridization analyses of genomic DNA isolated from the sea urchin Strongylocentrotus droebachiensis. Probes prepared from v-erbB, v-myc, c-myb and v-fps were found to hybridize with discrete fragments of HindIII digested genomic DNA. In contrast, probes prepared from v-abl, v-fos, v-sis, v-src, and v-mos either hybridized with multiple fragments, indicating non-specific binding, or failed to hybridize at all above background levels. These results clearly demonstrate the presence of proto-oncogene homologous sequences in the sea urchin genome.
Mifflin
D
D
Department of Biochemistry, Memorial University of Newfoundland, St John's, Canada.
Robinson
J J
JJ
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D012617:Sea Urchins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D004262:DNA Restriction Enzymes