[Immunocytochemical and biochemical studies of an Ewing sarcoma cell line: evidence for neural differentiation in vitro].
A cell line established from a Ewing's sarcoma removed from the fibula of a 19-year-old female was investigated for morphology and hormone sensitivity. Cells in culture expressed neurofilaments and showed positive immunostaining for neuron-specific enolase (NSE), Leu-7-antigen (HNK-1) and S-100 protein. The cells also contained PAS-positive, diastase-digestible cytoplasmic material, indicative of glycogen inclusions. Moreover, the cells responded to dopamine as well as to beta-adrenergic agonists and prostaglandins to stimulate cyclic AMP synthesis and 3H-glycogen hydrolysis. In conclusion, the morphological and biochemical data show evidence for a neural differentiation pattern of Ewing's sarcoma cells in vitro.
van Valen
F
F
Medizinische Klinik und Poliklinik C, Abt. für Endokrinologie und Rheumatologie, Universität Düsseldorf.
Prior
R
R
Wechsler
W
W
Jürgens
H
H
Schäfer
U
U
Keck
E
E
D000943:Antigens, Differentiation; D001859:Bone Neoplasms / Q000473:pathology*; D002454:Cell Differentiation; D002460:Cell Line; D002648:Child; D000242:Cyclic AMP / Q000378:metabolism; D005360:Fibula / Q000473:pathology; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007382:Intermediate Filaments / Q000648:ultrastructure; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D012512:Sarcoma, Ewing / Q000473:pathology*
D000943:Antigens, Differentiation; D009418:S100 Proteins; D006003:Glycogen; D000242:Cyclic AMP; D010751:Phosphopyruvate Hydratase
Immunzytochemische und biochemische Untersuchungen an einer Ewing-Sarkom-Zellinie: Hinweise für eine neurale in-vitro Differenzierung.