Differences in L-[3H]glutamate accumulation and endogenous L-glutamate content in synaptic vesicles from mice selectively bred for differences in ethanol sensitivity.
The ATP-stimulated accumulation of L-[3H]glutamate by whole brain synaptic vesicle preparations from long-sleep and short-sleep mice, lines selectively bred for difference in sleep time response to acute ethanol administration, was examined. L-[3H]Glutamate accumulation in vesicles from short-sleep mice was approximately twice that observed in vesicles from long-sleep mice at three glutamate loading concentrations. The vesicular content of endogenous L-glutamate in crude and enriched vesicle preparations from short-sleep mice was approximately 1.5-fold higher than in vesicles from long-sleep mice. The data suggest that L-glutamate associated with synaptic vesicles may serve a role in glutamate neurosecretion.
Disbrow
J K
JK
Ruth
J A
JA
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D008297:Male; D051379:Mice; D012890:Sleep / Q000187:drug effects; D013572:Synaptic Vesicles / Q000378:metabolism; D013997:Time Factors
D005971:Glutamates; D000431:Ethanol; D018698:Glutamic Acid; D000255:Adenosine Triphosphate