[Development of test systems for immunoenzyme analysis for the detection of the antigen of the transmissible gastroenteritis virus of swine].
Direct and indirect variants of enzyme immunoassay (EIA) were developed for the detection of specific antigen of swine transmissible gastroenteritis virus (TGEV). Preparation of IgG were obtained from highly specific hyperimmune sera of guinea pigs and gnotobiotic piglets and their optimal concentration for the tests were determined. For the direct EIA, a peroxidase conjugate was prepared from guinea pig IgG and its working dilution was determined. The developed EIA systems proved to be highly sensitive for the detection of TGEV antigen both in infected cell cultures and clinical specimens from sick piglets detecting 5 ng of the virus-specific protein.
Rukhadze
G G
GG
Aliper
T I
TI
Sergeev
V A
VA
Shcheglova
E Iu
EIu
Artiushin
S K
SK
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D003332:Coronaviridae / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005856:Germ-Free Life; D006168:Guinea Pigs; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000302:isolation & purification; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology*
D000956:Antigens, Viral; D007106:Immune Sera; D007074:Immunoglobulin G
Razrabotka test-sistem immunofermentnogo analiza dlia vyiavleniia antigena virusa transmissivnogo gastroénterita svineĭ.