Adrenocorticotropin binding activity of B16/C3 melanoma cells.
The binding of adrenocorticotropic hormone (ACTH) to B16/C3 murine melanoma cells was found to be specific and saturable. The binding capacity of the cells changed as a function of the age of the culture. Scatchard analysis revealed one class of high-affinity ACTH binding sites. The specificity of ACTH binding to the cells was tested by displacement experiments with human leukocyte interferon (alpha-IFN) and alpha-melanocyte stimulating hormone (alpha-MSH) as the competitors. Structure-activity relationship of ACTH, alpha-MSH and alpha-IFN was discussed.
Simić-Krstić
J
J
Institute for Biological Research, Belgrade, Yugoslavia.
Sevaljević
L
L
D000324:Adrenocorticotropic Hormone / Q000493:pharmacokinetics*; D000818:Animals; D001667:Binding, Competitive; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000324:Adrenocorticotropic Hormone