[Multiple hybridization in genome analysis. 1. Reaction of diamine and bisulfite with cytosine for the introduction of nonradioactive markers into DNA].
A two-step chemical method has been developed for the introduction of biotin and other low-molecular derivatives into DNA. The method is based on the interaction of aliphatic diamines with cytosine residues of the polynucleotide chain in the presence of sodium bisulfite with subsequent addition of biotin. DNA modified this way may be used as an effective probe for non-isotopic hybridization which can be used simultaneously with 32P-labelled probes.
Turchinskiĭ
M F
MF
Aĭnbinder
E I
EI
Sverdlov
E D
ED
D000818:Animals; D010582:Bacteriophage lambda / Q000378:metabolism; D001710:Biotin; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003596:Cytosine; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000378:metabolism; D003959:Diamines; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005796:Genes; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D013447:Sulfites; D013861:Thiocyanates
D004251:DNA Transposable Elements; D004279:DNA, Viral; D003959:Diamines; D005452:Fluoresceins; D007202:Indicators and Reagents; D013447:Sulfites; D013861:Thiocyanates; D001710:Biotin; D003596:Cytosine; D016650:Fluorescein-5-isothiocyanate; C009279:sodium bisulfite
Mnozhestvennaia gibridizatsiia v analize genmov. 1. Reaktsiia diaminov i bisul'fita s tsitozinom dlia vvedeniia v DNK neradioaktivnykh metok.