Long-term bone marrow culture as a stem cell source for transplantation.
Stem cells in bone marrow capable of inducing spleen colonies in lethally irradiated mice can be maintained in liquid culture for up to 100 days if an appropriate marrow-derived feeder layer is provided. Marrow from some mouse strains cannot be maintained in such cultures. Neither duration of culture nor in vitro cultivation of marrow on allogeneic feeder layer in any way modulates the occurrence of graft-versus-host disease. Cocultivation of marrow with allogeneic feeder layers does not induce tolerance to feeder-layer-type skin grafts in lethally irradiated syngenic recipients. Though an excellent source of stem cells, long-term marrow cultures require further modification before successful allogeneic marrow transplantation without graft-versus-host reaction can be carried out.
McMillen
M A
MA
Simmons
R L
RL
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002455:Cell Division; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006085:Graft Survival; D006086:Graft vs Host Disease / Q000517:prevention & control; D018380:Hematopoietic Stem Cell Transplantation; D051379:Mice; D008815:Mice, Inbred Strains; D011833:Radiation Injuries, Experimental; D016038:Skin Transplantation; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D013997:Time Factors