title: Effect of activation of the Epstein-Barr virus genome on expression of B cell differentiation antigens of Burkitt's lymphoma lines.
abstract: Using anti-human B cell monoclonal antibodies prepared against B1 (CD20), B2 (CD21), B4 (CD19), and BB-1 (B lymphoblast antigen-1), we compared the expression of B cell differentiation antigens on a Jijoye-P3HR-1 cell line family of Burkitt's lymphomas. The expression of BB-1 and B2 antigens was faint on P3HR-1 K cell line which is an Epstein-Barr virus (EBV) high producer. On the other hand, B1 and B4 antigens were strongly expressed on it. It was also found that BB-1 expression decreased on P3HR-1 cells after activation of intracellular EBV genes by treating chemically with tumor-promoting agent (TPA) and n-butyrate, or on Raji cells on superinfection with EBV. This decrease of BB-1 was blocked by the additional treatment with retinoic acid, an inhibitor of virus replication. Dual immunofluorescence staining analysis showed that the individual cell expressing EBV-associated antigens expressed BB-1 antigen only marginally. The relationship between the change in phenotypes of host B cells and the activation of the EBV genome is discussed.
authors.affiliation: Department of Microbiology, Fukui Medical School.
authors.forename: T
authors.initials: T
authors.lastname: Yokochi
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: T
authors.initials: T
authors.lastname: Miyadai
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte; D002051:Burkitt Lymphoma / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D005814:Genes, Viral / Q000187:drug effects; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D010641:Phenotype; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D002087:Butyrates; D020148:Butyric Acid; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate