Genome structure of a new avian sarcoma virus PR2257 determined by restriction analysis.
The genome structure of a new avian src-containing acute transforming virus, designated PR2257, was studied by restriction analysis. PR2257 was isolated from a sarcoma grown in a 155-day-old chick inoculated intraembryonally with a transformation-defective mutant of daPR-C retaining no src sequences. The 4.4-kb genome of PR2257 consists of the following regions: 5' LTR--spliced version of chicken c-src--incomplete env gene--3' LTR. This structure clearly indicates that PR2257 was generated by recombination between a td daPR-C and chicken c-src.
Nehyba
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Svoboda
J
J
Karakoz
I
I
Rynditch
A V
AV
Geryk
J
J
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003370:Coturnix; D004625:Embryo, Nonmammalian; D005347:Fibroblasts / Q000166:cytology; D009858:Oncogenic Viruses; D015183:Restriction Mapping