[SH-groups of NAD kinase from the rabbit liver].
Two SH-groups per enzyme subunit have been identified in the native preparation of rabbit liver NAD kinase, using DTNB. The titration curve is biphasic; one SH-group is modified at each step. There is a strict correlation between the loss of the enzyme activity and the rate of modification of fast and slow SH-groups. Substrates afford only a partial protection of NAD kinase against the DTNB-induced inactivation. The data obtained suggest that two SH-groups of NAD kinase are essential for the enzyme activity; however, these groups are not directly involved in the active center formation.
Bulygina
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Voronina
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Tsyplenkova
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D000818:Animals; D002384:Catalysis; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology; D010770:Phosphotransferases / Q000032:analysis*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011817:Rabbits; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis*
D013438:Sulfhydryl Compounds; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase
Issledovanie SH-grupp NAD-kinazy iz pecheni krolika.