Activation of dolichol-phosphate mannosyltransferase by dibutryl cyclic AMP in rat liver.
Radiolabeled mannose incorporation into secretory glycoproteins and immunoprecipitable fibronectin in the incubation media significantly increased (105 and 32 percent respectively) with a corresponding increase in the levels of dolichol-phosphate mannose, dolichol-diphosphate oligosaccharides and dolichol-phosphate mannosyltransferase activity in the rat liver slices when incubated with dibutryl cAMP and ATP. Dibutryl cAMP activated maximally this enzyme in the presence of ATP in the incubation medium. The activation of the enzyme resulted in a two fold increase in Vmax with no apparent change in the Km for GDP mannose. Phosphorylation the rat liver microsomes with catalytic subunit of cAMP dependent protein kinase, resulted in the activation of dolichol-phosphate mannosyltransferase. These results suggest that cAMP modulates protein glycosylation by activating dolicholphosphate mannosyltransferase activity. The activation of this enzyme could be through phosphorylation/dephosphorylation mechanism involving a cAMP dependent protein kinase.
Sharma
C
C
Department of Medicine, Louisiana State University Medical Center, New Orleans 70112.
Radhakrishnamurthy
B
B
Berenson
G S
GS
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D004287:Dolichol Monophosphate Mannose / Q000096:biosynthesis; D004288:Dolichol Phosphates / Q000378:metabolism; D004789:Enzyme Activation; D006031:Glycosylation; D006602:Hexosyltransferases / Q000378:metabolism*; D008297:Male; D008364:Mannosyltransferases / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D004288:Dolichol Phosphates; C026406:dolichol pyrophosphate; D004287:Dolichol Monophosphate Mannose; D003994:Bucladesine; D000255:Adenosine Triphosphate; D006602:Hexosyltransferases; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase