Studies on turnover rates of rat gamma-glutamyltranspeptidase after chronic ethanol administration in vivo.
Chronic ethanol administration to rats was shown to result in a significant increase of hepatic and serum GGT activities, contrasting to the decreased levels observed in pancreas, intestine, brain, and kidney by the new alcock regimen method. The kinetics of rat GGT synthesis and degradation in vivo among the different sources after chronic ethanol administration has been studied by use of acivicin, which irreversibly inactivates GGT. The comparison of kinetics of GGT return after acivicin injection showed that the kidney and serum GGT exhibits biphasic half-lives in contrast to liver, pancreatic, intestinal, and brain GGT half-lives in chronic ethanol-administered rats. The present studies on kinetics of GGT synthesis (Ks) and degradation (Kd) in vivo would seem to indicate the existence of three types of systems. That is, Ks rather than Kd may be preferential in liver and serum whereas Kd is apparently increased in kidney and intestine without noticeable change in Ks. The reverse phenomenon is also observed for pancreas and brain. These findings suggest that the contributions of alterations in the rates of GGT synthesis and degradation to changing levels of GGT have been evaluated as a mechanism for enzyme adaptation in animal tissues as a change from the control diet to the ethanol diet.
Rambabu
K
K
Matsuda
Y
Y
Katunuma
N
N
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001921:Brain / Q000201:enzymology; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006207:Half-Life; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology; D007555:Isoxazoles / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D000963:Antimetabolites; D007555:Isoxazoles; D000431:Ethanol; D005723:gamma-Glutamyltransferase; C006303:acivicin