title: Characterization of DNA fragments encoding fimbriae of the uropathogenic Escherichia coli strain KS71.
abstract: Recombinant plasmids were constructed that expressed the KS71A, KS71B and KS71C fimbrial antigens of the pyelonephritogenic Escherichia coli strain KS71 (O4:K12) in E. coli HB101. The KS71C-encoding genes were located on a 6.4 kb HindIII-XhoI fragment obtained from the recombinant cosmid pKTH145 that expresses this antigen. Spontaneous KS71C-mutants were isolated that contained a 0.8 kb insert in a specific restriction fragment of KS71C-encoding recombinant plasmids. The KS71B-encoding segment was located on a 11.5 kb deletable DNA fragment of recombinant cosmid pKTH144. A DNA fragment encoding the KS71A fimbria was obtained on a 12 kb EcoRI fragment of the recombinant cosmid expressing this antigen in E. coli HB101 and closely resembled the KS71B-encoding fragment. In the recombinant cosmid, the KS71B-expressing region was flanked by homologous DNA segments. A similar stretch of DNA was found close to the KS71A-expressing DNA region.
authors.forename: M
authors.initials: M
authors.lastname: Rhen
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D010861:Fimbriae, Bacterial; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes