title: Calcium-dependent transglutaminase of rat sympathetic ganglion in development and after nerve injury.
abstract: The activity of transglutaminase (TG) was examined in the rat superior cervical ganglion (SCG) during development and after postganglionic nerve crush. During postnatal development the enzyme activity is increased by sevenfold in parallel to protein content of the ganglion and reaches adult levels by day 35 after birth. The endogenous activity (enzyme activity assayed in the absence of the exogenous substrate) during development is transiently elevated with a peak at day 21 postnatal. In the adult ganglion the enzyme specific activity is evenly distributed in all subcellular compartments, but most of it is contained in the cytosol. Within the first hour after axotomy TG activity is rapidly and transiently elevated. The peak value, 80% above control levels, is attained by 30 min postoperative. At this time the activity is increased in all subcellular fractions, but the endogenous activity is selectively increased in the fraction containing nuclei. The enhanced TG activity after axotomy can be prevented by topical treatments with verapamil, an inhibitor of voltage-dependent calcium fluxes across excitable membranes, or with the calcium chelator EGTA. The results show that intracellular TG activity is present in the SCG and that it increases with postnatal growth of the ganglion. After axotomy the enzyme activity is rapidly and transiently increased in the ganglion and this elevation critically depends on calcium fluxes.
authors.forename: G M
authors.initials: GM
authors.lastname: Gilad
authors.forename: L E
authors.initials: LE
authors.lastname: Varon
authors.forename: V H
authors.initials: VH
authors.lastname: Gilad
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004533:Egtazic Acid / Q000494:pharmacology; D005260:Female; D005728:Ganglia, Sympathetic / Q000201:enzymology* / Q000254:growth & development; D008297:Male; D009409:Nerve Crush; D051381:Rats; D011919:Rats, Inbred Strains; D011503:Transglutaminases; D014700:Verapamil / Q000494:pharmacology
chemical_list: D004533:Egtazic Acid; D014700:Verapamil; D000217:Acyltransferases; D011503:Transglutaminases; D002118:Calcium