Vesicular stomatitis virus M protein in the nuclei of infected cells.
The M protein of vesicular stomatitis virus (VSV) was localized in the nuclei and cytoplasm of VSV-infected cells by subcellular fractionation and immunofluorescence microscopy. Nuclei isolated from VSV-infected Friend erythroleukemia cells were fractionated into a nuclear membrane and a nucleoplasm fraction by DNase digestion and differential centrifugation. G protein was present in the membrane fraction, and M protein was present in the nucleoplasm fraction. Immunofluorescence detection of M protein in the nucleus required that fixed cells be permeabilized with higher concentrations of detergent than were required for detection of M protein in the cytoplasm of VSV-infected BHK cells.
Lyles
D S
DS
Department of Microbiology and Immunology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103.
Puddington
L
L
McCreedy
B J
BJ
D000911:Antibodies, Monoclonal; D002463:Cell Membrane Permeability / Q000187:drug effects; D002467:Cell Nucleus / Q000032:analysis*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D013048:Specimen Handling; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis*; D014763:Viral Matrix Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D014763:Viral Matrix Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol