Hemolysin production by Leptospira interrogans serovar canicola in a protein-free medium with hemin.
Leptospira interrogans serovar canicola strain moulton was grown to a high cell density in a protein-free medium. When hemin was added to this medium, hemolysin was produced. Hemolysin was not detected when other porphyrins or cytochrome C were substituted for hemin in the medium.
Lane
E M
EM
Department of Medical Microbiology, University of Nebraska College of Medicine, Omaha 68105.
Ramaley
R F
RF
Miller
N G
NG
D003470:Culture Media; D003574:Cytochrome c Group / Q000494:pharmacology; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D007921:Leptospira interrogans / Q000254:growth & development / Q000378:metabolism*; D041341:Leptospira interrogans serovar canicola / Q000254:growth & development / Q000378:metabolism; D011166:Porphyrins / Q000494:pharmacology; D011506:Proteins
D003470:Culture Media; D003574:Cytochrome c Group; D006460:Hemolysin Proteins; D011166:Porphyrins; D011506:Proteins; D006418:Heme; D006427:Hemin