A comparison of bovine growth-hormone gene expression in mouse L cells directed by the Moloney murine-leukemia virus long terminal repeat, simian virus-40 early promoter or cytomegalovirus immediate-early promoter.
Three transcriptional regulatory regions including a human cytomegalovirus immediate-early region (CMVIE) promoter, the simian virus-40 early region (SV40E) promoter, and Moloney murine leukemia virus (MoMLV) long terminal repeat (LTR) were ligated to the bovine growth hormone (bGH)-coding gene. Using a bGH transient expression system, the CMVIE and SV40E gene transcriptional regulatory regions were found to be approximately two-fold more efficient than the MoMLV LTR in directing expression of bGH in mouse L cells.
Martin-Gallardo
A
A
Department of Zoology and Biomedical Sciences, Edison Animal Biotechnology Center, Athens, OH.
Montoya-Zavala
M
M
Kelder
B
B
Taylor
J
J
Chen
H
H
Leung
F C
FC
Kopchick
J J
JJ
D000818:Animals; D002417:Cattle; D003587:Cytomegalovirus / Q000235:genetics; D004274:DNA, Recombinant; D005809:Genes, Regulator; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics*; D007739:L Cells; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
D004274:DNA, Recombinant; D013006:Growth Hormone