Evaluation of Abbott CMV-M enzyme immunoassay for detection of cytomegalovirus immunoglobulin M antibody.
The Abbott CMV-M enzyme immunoassay (EIA) for the qualitative determination of immunoglobulin M (IgM) antibody to cytomegalovirus in human serum was compared with the indirect fluorescent-antibody (IFA) test on 338 human serum specimens. Discordant specimens were evaluated by IFA following isolation of IgM fractions. Discordant specimens remaining after IFA testing were evaluated by an IgM-specific EIA (CYTOMEGELISA M; M.A. Bioproducts). After resolution of discordant specimens, the CMV-M EIA was 94.7% sensitive and 99.1% specific.
Schaefer
L
L
Diagnostics Division, Abbott Laboratories, Abbott Park, Illinois 60064.
Cesario
A
A
Demmler
G
G
Plotkin
S
S
Stagno
S
S
Elrod
C
C
Ringler
J
J
Tustin
N
N
D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology; D005069:Evaluation Studies as Topic; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007108:Immune Tolerance; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*; D011247:Pregnancy
D000914:Antibodies, Viral; D007075:Immunoglobulin M