Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma.
Two overlapping clones, covering the entire coding sequence of human M2-type pyruvate kinase (PK) cDNA, were isolated and sequenced. Nucleotide sequencing results showed that they contained the 109-bp 5'-untranslated region, the 1593-bp coding region and the 585-bp 3'-untranslated region. Nucleotide sequence homology was 90% and 69% with rat M2-type and L-type PK cDNA, respectively. In situ hybridization using the human M2-type PK cDNA probe disclosed that the gene for M2-type PK is located at band q22 on chromosome 15. Northern blot analysis with RNA from human hepatoma demonstrated that M2-type PK was predominantly expressed in hepatoma cells, whereas L-type PK was preferentially expressed in the non-tumor portion of the liver.
Tani
K
K
Department of Internal Medicine, University of Tokyo, Japan.
Yoshida
M C
MC
Satoh
H
H
Mitamura
K
K
Noguchi
T
T
Tanaka
T
T
Fujii
H
H
Miwa
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics*; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000302:isolation & purification; D005796:Genes; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011770:Pyruvate Kinase / Q000235:genetics*; D015183:Restriction Mapping
D004274:DNA, Recombinant; D007527:Isoenzymes; D011770:Pyruvate Kinase