Phosphorylation of the rev gene product of human immunodeficiency virus type 1.
Replication of human immunodeficiency virus type 1 requires the functional expression in trans of the virally encoded rev gene product (previously called art/trs). Here we demonstrate that this protein can be metabolically labeled with 32Pi. The phosphate receptor in the rev protein is shown to be exclusively serine. Treatment of rev-expressing cells with phorbol ester, a specific activator of protein kinase C, led to significant but transient enhancement of the level of rev phosphorylation. These results indicate that the rev protein is posttranslationally modified in vivo and suggest that the level of this modification is subject to modulation by extracellular stimuli.
Hauber
J
J
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
Bouvier
M
M
Malim
M H
MH
Cullen
B R
BR
D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D015694:Gene Products, rev; D015696:Gene Products, tat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D010766:Phosphorylation; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D015696:Gene Products, tat; D014157:Transcription Factors; D014764:Viral Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus