Evidence in volume-dependent hypertension for an augmenting factor for norepinephrine overflow from sympathetic nerve endings.
It has been reported that salt loading induces a blood pressure rise in rats whose renal mass has been reduced. We examined the involvement of the peripheral sympathetic nervous system in the pathogenesis of hypertension in subtotally nephrectomized and salt-loaded rats. Male Wistar rats (200-240 g) underwent subtotal nephrectomy (removal of 70-80% of the renal mass). After surgery, 1% saline was given ad libitum as drinking water in the experimental group (E), while tap water was given to the controls (C). On the 10th day after nephrectomy, blood pressure was determined, and plasma samples were collected. Deproteinized plasma samples were diluted with Ringer-Locke solution (1/40 v/v), and were perfused into isolated mesenteric artery-intestinal loop preparations of normotensive male Wistar rats. Pressor responses and norepinephrine overflow induced by electrical nerve stimulation, and the pressor responses to exogenous norepinephrine were assessed before and after the addition of deproteinized plasma to the perfusate. Systolic blood pressure was significantly elevated in the experimental group (E: 150 +/- 5 mmHg, C: 109 +/- 4 mmHg, p less than 0.01). When deproteinized plasma from the experimental group was perfused, pressor responses to electrical nerve stimulation were significantly increased (E: 167.2 +/- 13.7%, C: 91.1 +/- 6.4%; p less than 0.01), as was norepinephrine overflow (E: 117.3 +/- 6.4%, C: 90.7 +/- 5.9%; p less than 0.05), while responses to exogenous norepinephrine were only slightly augmented compared with the control group (E: 120.0 +/- 2.7%, C: 105.5 +/- 9.0%; n.s.).(ABSTRACT TRUNCATED AT 250 WORDS)
Shima
H
H
Department of Medicine, Wakayama Medical College, Japan.
Nishio
I
I
Tsuda
K
K
Kimura
K
K
Takeda
J
J
Masuyama
Y
Y
D000818:Animals; D001794:Blood Pressure; D004558:Electric Stimulation; D006973:Hypertension / Q000503:physiopathology*; D008297:Male; D008638:Mesenteric Arteries; D009392:Nephrectomy; D009411:Nerve Endings / Q000503:physiopathology; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000097:blood; D012965:Sodium Chloride / Q000008:administration & dosage; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013564:Sympathetic Nervous System / Q000503:physiopathology*
D012965:Sodium Chloride; D012083:Renin; D000254:Sodium-Potassium-Exchanging ATPase; D009638:Norepinephrine