Removal of Arg1 and Phe22 from CLIP (ACTH18-39) by rodent pituitary and blood peptidases.
The major product on incubation of CLIP (ACTH18-39) with rat and mouse serum, rat plasma and whole blood, and soluble extracts of rat pituitary is [des-Arg1]-CLIP (ACTH19-39) while [des-Phe22]-CLIP (ACTH18-38) is the major product with pituitary particulate fraction. In both cases, p-chloromercuribenzoate-sensitive, metal-dependent peptidase activity appears to be responsible for the cleavage. The serum enzyme may be related to proline aminopeptidase. Material coeluting with [des-Arg]-CLIP on two HPLC solvent gradients is present in the superfusion media from neurointermediate lobes of genetically obese (ob/ob) mice but is not present in acid extracts of the lobe. This suggests that postsecretory processing of CLIP may involve removal of the N-terminal Arg residue.
McDermott
J R
JR
MRC Neuroendocrinology Unit, Newcastle General Hospital.
Biggins
J A
JA
Smith
A I
AI
Gibson
A M
AM
Keith
A B
AB
Edwardson
J A
JA
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine; D053497:Corticotropin-Like Intermediate Lobe Peptide; D051379:Mice; D008820:Mice, Obese; D010446:Peptide Fragments / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000097:blood / Q000378:metabolism*; D010649:Phenylalanine; D010902:Pituitary Gland / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D000596:Amino Acids; D010446:Peptide Fragments; D011480:Protease Inhibitors; D010649:Phenylalanine; D053497:Corticotropin-Like Intermediate Lobe Peptide; D000324:Adrenocorticotropic Hormone; D001120:Arginine; D010447:Peptide Hydrolases