title: Identification and characterization of TnphoA mutants of Salmonella that are unable to pass through a polarized MDCK epithelial cell monolayer.
abstract: Surface protein mutants of the invasive Salmonella species, S. choleraesuis, were generated using the transposon TnphoA. 626 alkaline phosphatase (PhoA+) fusion mutants were identified and screened for their ability to pass through (transcytose) polarized epithelial monolayers of Madin Darby canine kidney (MDCK) cells grown on membrane filters. Forty two mutants were unable to pass through this barrier. All of these transcytosis mutants were unable to adhere to or invade MDCK monolayers, yet these mutations were not in the genes encoding type 1 pili or mannose-resistant haemagglutination (MRHA). These transcytosis mutants could be grouped into six classes. Class 1 mutants had altered lipopolysaccharide (LPS) O side-chain structures while Class 2 mutants had defects in their LPS core. Mutants belonging to Classes 5 and 6 did not decrease the transepithelial electrical resistance of polarized MDCK cell monolayers, in contrast to the parental strain and the other mutants (Classes 1, 2, 3 and 4). Mutants belonging to Class 1 were less virulent in mice, while Class 2 (defective core) and Classes 4 and 5 (normal LPS) mutant strains were avirulent in mice. Mutants from Classes 3 and 6 were as virulent in mice as S. choleraesuis. These results suggest that the ability to pass through epithelial barriers may be an important virulence characteristic of Salmonella. These data indicate that bacterial adherence, internalization and monolayer transcytosis are closely linked events. It was also demonstrated that a mutant with decreased rates of intracellular replication still passed through the monolayer at rates similar to wild-type S. choleraesuis.
authors.affiliation: Department of Medical Microbiology, Stanford University School of Medicine, California 94305.
authors.forename: B B
authors.initials: BB
authors.lastname: Finlay
authors.forename: M N
authors.initials: MN
authors.lastname: Starnbach
authors.forename: C L
authors.initials: CL
authors.lastname: Francis
authors.forename: B A
authors.initials: BA
authors.lastname: Stocker
authors.forename: S
authors.initials: S
authors.lastname: Chatfield
authors.forename: G
authors.initials: G
authors.lastname: Dougan
authors.forename: S
authors.initials: S
authors.lastname: Falkow
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics*; D000818:Animals; D001422:Bacterial Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008070:Lipopolysaccharides / Q000235:genetics; D051379:Mice; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012475:Salmonella / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D014774:Virulence
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase