title: Relationship between sensitivity to 4'-(9-acridinylamino)methanesulfon-m-anisidide and DNA topoisomerase II in a cold-sensitive cell-cycle mutant of a murine mastocytoma cell line.
abstract: The cold-sensitive (proliferating at 39.5 degrees C, reversibly arrested in GI-phase at 33 degrees C) cell-cycle mutant 21-Fb of the murine mastocytoma cell line P815 was used to study the effect of amsacrine on non-cycling cells. The sensitivity of arrested 21-Fb cells decreased less than 2-fold in cell survival experiments when compared to proliferating cells. In contrast, DNA breakage and stimulation of protein-DNA complex formation in intact or lysed cells was reduced approx. 10-fold in arrested cells and DNA topoisomerase II activity in arrested cells was only 5% of the activity in proliferating cells. Thus, there was no correlation between cell survival and DNA damage or DNA topoisomerase II activity in drug-treated cells.
authors.affiliation: Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
authors.forename: E
authors.initials: E
authors.lastname: Schneider
authors.forename: A M
authors.initials: AM
authors.lastname: Hutchins
authors.forename: S J
authors.initials: SJ
authors.lastname: Darkin
authors.forename: P A
authors.initials: PA
authors.lastname: Lawson
authors.forename: R K
authors.initials: RK
authors.lastname: Ralph
mesh_terms: D000677:Amsacrine / Q000494:pharmacology*; D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002470:Cell Survival / Q000187:drug effects; D003080:Cold Temperature; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D012515:Mast-Cell Sarcoma; D051379:Mice; D009154:Mutation; D011506:Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D011506:Proteins; D000677:Amsacrine; D004247:DNA; D004250:DNA Topoisomerases, Type II