title: Cerebral ammonia metabolism in hyperammonemic rats.
abstract: The short-term metabolic fate of blood-borne [13N]ammonia was determined in the brains of chronically (8- or 14-week portacaval-shunted rats) or acutely (urease-treated) hyperammonemic rats. Using a "freeze-blowing" technique it was shown that the overwhelming route for metabolism of blood-borne [13N]ammonia in normal, chronically hyperammonemic and acutely hyperammonemic rat brain was incorporation into glutamine (amide). However, the rate of turnover of [13N]ammonia to L-[amide-13N]glutamine was slower in the hyperammonemic rat brain than in the normal rat brain. The activities of several enzymes involved in cerebral ammonia and glutamate metabolism were also measured in the brains of 14-week portacaval-shunted rats. The rat brain appears to have little capacity to adapt to chronic hyperammonemia because there were no differences in activity compared with those of weight-matched controls for the following brain enzymes involved in glutamate/ammonia metabolism: glutamine synthetase, glutamate dehydrogenase, aspartate aminotransferase, glutamine transaminase, glutaminase, and glutamate decarboxylase. The present findings are discussed in the context of the known deleterious effects on the CNS of high ammonia levels in a variety of diseases.
authors.forename: A J
authors.initials: AJ
authors.lastname: Cooper
authors.forename: S N
authors.initials: SN
authors.lastname: Mora
authors.forename: N F
authors.initials: NF
authors.lastname: Cruz
authors.forename: A S
authors.initials: AS
authors.lastname: Gelbard
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000641:Ammonia / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000032:analysis; D005974:Glutamate-Ammonia Ligase / Q000032:analysis; D005971:Glutamates / Q000032:analysis; D018698:Glutamic Acid; D008297:Male; D009590:Nitrogen Radioisotopes; D011167:Portacaval Shunt, Surgical; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005971:Glutamates; D009590:Nitrogen Radioisotopes; D018698:Glutamic Acid; D000641:Ammonia; D000255:Adenosine Triphosphate; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase