Interaction of calcium-binding proteins with calmodulin-dependent guanylate cyclase in Tetrahymena plasma membrane.
Addition of bovine brain calmodulin and S-100 inhibited Tetrahymena calmodulin-induced stimulation of guanylate cyclase, but they did not affect enzymatic activity in the presence of calcium alone. Troponin C shows little effect on the cyclase activity regardless of the presence or absence of Tetrahymena calmodulin. The inhibitory effects of brain calmodulin and S-100 were overcome by the addition of Tetrahymena calmodulin, but not by calcium. Both calmodulins from Tetrahymena and bovine brain elicited stimulation of heart phosphodiesterase, while troponin C and S-100 did not affect the phosphodiesterase activity in the presence and absence of Tetrahymena calmodulin.
Kudo
S
S
Muto
Y
Y
Inagaki
M
M
Hidaka
H
H
Nozawa
Y
Y
D000818:Animals; D001923:Brain Chemistry; D002118:Calcium / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology*; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009418:S100 Proteins / Q000494:pharmacology; D013769:Tetrahymena pyriformis / Q000201:enzymology*; D014336:Troponin / Q000494:pharmacology; D019209:Troponin C
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D009418:S100 Proteins; D014336:Troponin; D019209:Troponin C; D006162:Guanylate Cyclase; D002118:Calcium