In vitro Sendai viral replication in pulmonary macrophages from mice treated with 4-ipomeanol.
The ability of pulmonary macrophages from female mice given 4-ipomeanol (4-IP) to support viral replication in vitro was examined. On posttreatment days 5, 7, and 9, greater numbers of macrophages were recovered in lavage fluid from lungs of mice given 4-IP, compared with those from controls. Pulmonary macrophages recovered in lavage fluid from control mice and from mice given 4-IP supported Sendai virus replication in vitro, and nearly all Sendai virus produced in pulmonary macrophages from both groups was in an infectious form. On posttreatment days 5 and 7, slightly greater absolute numbers of virions were produced in pulmonary macrophages recovered from mice given 4-IP.
Durham
S K
SK
Department of Pathology and Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Dubovi
E A
EA
Castleman
W L
WL
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology*; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development / Q000502:physiology*; D011897:Random Allocation; D013729:Terpenes / Q000494:pharmacology*; D010948:Viral Plaque Assay; D014779:Virus Replication
D013729:Terpenes; C001470:4-ipomeanol