Identification and characterization of prostaglandin-binding components in rat ileum.
A specific prostaglandin E2 (PGE2) binding component was identified in the membrane fractions of rat ileum. Two ligands, 3H-PGE2 and 14C-PGE2, competed for the binding sites in crude homogenates of ileum during competition experiments, demonstrating the presence of a limited number of binding sites. There was enhancement in the specific 3H-PGE2 binding to particulate fractions over control when endogenous prostaglandin synthesis was blocked by the administration of indomethacin to rats prior to sacrifice. The specific prostaglandin-binding protein was purified by a combination of [NH4]2SO4 fractionation and Sephacryl S-200 column chromatographic techniques, and shown to be active after these biochemical steps.
Berko
R M
RM
Dubin
A
A
D000818:Animals; D001667:Binding, Competitive; D015232:Dinoprostone; D007082:Ileum / Q000032:analysis / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D011458:Prostaglandins E / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D011982:Receptors, Prostaglandin / Q000302:isolation & purification* / Q000378:metabolism; D018078:Receptors, Prostaglandin E
D011458:Prostaglandins E; D011956:Receptors, Cell Surface; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D015232:Dinoprostone; D007213:Indomethacin