Discrimination of gamma-glutamyltranspeptidase from normal and carcinomatous pancreas.
Two types of gamma-glutamyltranspeptidase (gamma-GTP) were identified in normal human pancreas, but ductal cell carcinomas of the pancreas showed only one type on analysis with polyacrylamide gel electrophoresis, anion exchange column chromatography, concanavalin A affinity chromatography and isoelectric focusing. gamma-GTP from carcinoma tissue was almost identical to the sialic acid-rich type of normal pancreatic gamma-GTP, and our methods did not evidence a difference between the two. However, E-PHA lectin column chromatography demonstrated differences in their elution pattern. Compared with normal pancreatic gamma-GTPs, the elution pattern of the carcinoma gamma-GTP was slowly delayed by the E-PHA lectin column. This finding suggests the presence of a bisecting N-acetylglucosamine-rich sugar chain in pancreatic carcinoma gamma-GTP, as well as the possible presence of a different sugar structure in the carcinoma gamma-GTP.
Yamaguchi
N
N
Kawai
K
K
Ashihara
T
T
D000117:Acetylglucosamine / Q000032:analysis; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D007526:Isoelectric Point; D037102:Lectins / Q000378:metabolism; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology*; D010190:Pancreatic Neoplasms / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D037102:Lectins; D005723:gamma-Glutamyltransferase; D000117:Acetylglucosamine