title: Characterization of cytochrome c oxidase III transcripts that are edited only in the 3' region.
abstract: The cytochrome c oxidase subunit III (COIII) transcript of T. brucei is extensively edited by the addition and deletion of uridines. We have identified partially edited COIII RNAs; they have unedited 5' and edited 3' regions. Transcripts with edited 5' but unedited 3' regions were not detected. The partially edited RNAs may be editing intermediates. The junctions of unedited and edited sequences in cDNAs differ from both the DNA and fully edited mRNA sequences over a multinucleotide region. The partially edited RNAs could arise from several processes, but it seems likely that editing proceeds in the 3' to 5' direction.
authors.affiliation: Seattle Biomedical Research Institute, Washington 98109-1651.
authors.forename: J M
authors.initials: JM
authors.lastname: Abraham
authors.forename: J E
authors.initials: JE
authors.lastname: Feagin
authors.forename: K
authors.initials: K
authors.lastname: Stuart
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D003576:Electron Transport Complex IV / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D003576:Electron Transport Complex IV