Properties of rabbit erythrocytes treated with phospholipase A2 from bee venom.
In isotonic saline-buffered conditions, phospholipase A2 from bee venom cleaves up to 65% of the phosphatidylcholine of the rabbit RBC membrane without causing significant hemolysis; however, the volume and the osmotic fragility of the treated RBC is modified. The osmotic behaviour of PLA-treated RBC after reincubation in autologous plasma suggests an heterogeneity in the enzymatic attack among the RBC population. In vivo RBC survival is strongly impaired by PLA treatment.
Vaysse
J
J
Pilardeau
P
P
Garnier
M
M
D000818:Animals; D001514:Bee Venoms; D004910:Erythrocyte Membrane / Q000032:analysis; D004912:Erythrocytes / Q000187:drug effects*; D007700:Kinetics; D008563:Membrane Lipids / Q000097:blood*; D009996:Osmotic Fragility / Q000187:drug effects*; D010740:Phospholipases / Q000494:pharmacology*; D010741:Phospholipases A / Q000378:metabolism / Q000494:pharmacology*; D054467:Phospholipases A2; D010743:Phospholipids / Q000097:blood*; D011817:Rabbits
D001514:Bee Venoms; D008563:Membrane Lipids; D010743:Phospholipids; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2