Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis.
Cloning vector plasmids have been constructed on the basis of the broad host range plasmid pAM beta 1 and used for the cloning of a nisin resistance determinant in Streptococcus lactis. They incorporate several desirable features for gene cloning in S. lactis and other transformable Gram-positive bacteria. They carry an easily selectable erythromycin resistance marker, are present at low (6-9) or high (45-85) copy number in S. lactis and possess a convenient polyrestriction site sequence. A significant advantage of these plasmids is their capability to carry and stably maintain very large cloned DNA fragments (up to 30 kilobases).
Simon
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Institut National de la Recherche Agronomique, Laboratoire de Recherches de Technologie Laitière, Rennes, France.
Chopin
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D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial / Q000235:genetics; D004917:Erythromycin; D005798:Genes, Bacterial; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000235:genetics*; D009561:Nisin; D010957:Plasmids; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D009561:Nisin; D004917:Erythromycin; D004262:DNA Restriction Enzymes