Changes in glycan branching and sialylation of the Thy-1 antigen during normal differentiation of mouse T-lymphocytes.
The glycans of the Thy-1 antigen present on thymocytes and lymph-node T-lymphocytes were investigated after external labelling of the cells. Neuraminidase, endoglycosidase H and endoglycosidase F were used in combination with sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and isoelectric focusing in order to characterize the nature of the glycans on 125I-labelled and immunoprecipitated Thy-1. Glycopeptides were prepared from Thy-1 obtained from cells labelled by periodate/boro[3H]hydride treatment. The glycopeptides were separated by affinity chromatography on concanavalin A-Sepharose and analysed by gel filtration. The results show that both types of cells possess Thy-1 molecules with three N-linked carbohydrate chains, of which one is of 'high-mannose' type and the other two of triantennary and biantennary 'complex' type. The ratio of triantennary/biantennary chains was decreased on Thy-1 of mature cells compared with that of immature cells, but instead more sialic acid was present on these chains. Deglycosylated Thy-1 appeared to be of the same size regardless of origin, indicating that only the carbohydrate moiety differs between Thy-1 molecules of the two cell types.
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D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D002454:Cell Differentiation; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006020:Glycopeptides / Q000032:analysis; D006026:Glycoside Hydrolases; D007525:Isoelectric Focusing; D051379:Mice; D008809:Mice, Inbred C3H; D019158:N-Acetylneuraminic Acid; D011134:Polysaccharides / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000276:immunology
D000954:Antigens, Surface; D006020:Glycopeptides; D011134:Polysaccharides; D012794:Sialic Acids; D018800:Thy-1 Antigens; D006026:Glycoside Hydrolases; D019158:N-Acetylneuraminic Acid