title: A synthetic peptide corresponding to the cleavage region of VP3 from rotavirus SA11 induces neutralizing antibodies.
abstract: Antibodies were elicited in rabbits by immunization with the synthetic tetradecapeptide Gln-Asn-Thr-Arg-Asn-Ile-Val-Pro-Val-Ser-Ile-Val-Ser-Arg, corresponding to amino acids 228 to 241 of SA11-VP3. Protein specificity of the antipeptide serum is demonstrated. The antipeptide serum revealed neutralizing activity directed against SA11 in a neutralization assay. Human rotavirus strains Wa, S2, and Hochi and bovine strains NCDV and UK were not neutralized, demonstrating the strain-specific neutralizing activity of the raised antipeptide serum. Upon immune electron microscopy, aggregation of SA11 particles was observed.
authors.affiliation: Abteilung für Medizinische Mikrobiologie und Virologie, Ruhr-Universität Bochum, Federal Republic of Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Streckert
authors.forename: H
authors.initials: H
authors.lastname: Brüssow
authors.forename: H
authors.initials: H
authors.lastname: Werchau
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006389:Hemagglutinins, Viral / Q000276:immunology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D012401:Rotavirus / Q000276:immunology* / Q000648:ultrastructure; D014612:Vaccines; D014614:Vaccines, Synthetic; D014764:Viral Proteins / Q000276:immunology*; D015678:Viral Structural Proteins
chemical_list: D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D010446:Peptide Fragments; D014612:Vaccines; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D015678:Viral Structural Proteins