title: Atrial-specific granules in the hearts of normal and water-deprived rats.
abstract: The morphology of atrial-specific granules, which contain atrial natriuretic polypeptide (ANP), was studied in the cardiac tissue of untreated controls and water-deprived rats by means of conventional and immunoelectron microscopy. Immature secretory vesicles or granules appeared to become buded off from the Golgi cisternae and then fused to form specific A-granules. An electron-dense plate with a fuzzy coat was frequently found on the limiting membrane at the end of such fusion. Pale specific B-granules, which were less electron-dense, larger, and more granular than A-granules, were found in small numbers in the left atrial cardiocytes, but rarely in the right ones. Very pale granules with a less granular matrix, considered to be B-type granules which had lost their electron-density, and which had less immunoreactivity for ANP, were numerous in the cardiac tissue after water deprivation. This morphological change, which is interpreted as an indication of granule degradation, was in agreement with the noted increase of natriuretic activity in the atrial tissue of water-deprived specimens.
authors.affiliation: Department of Internal Medicine, Miyazaki Medical College, Miyazaki Prefectural Hospital, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Toshimori
authors.forename: K
authors.initials: K
authors.lastname: Toshimori
authors.forename: S
authors.initials: S
authors.lastname: Matsukura
authors.forename: C
authors.initials: C
authors.lastname: Oura
authors.forename: H
authors.initials: H
authors.lastname: Matsuo
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000276:immunology; D006321:Heart / Q000502:physiology; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D014868:Water Deprivation / Q000502:physiology*
chemical_list: D009320:Atrial Natriuretic Factor