Circulating histamine and neutrophil chemotactic activity during allergen-induced asthma: the effect of inhaled antihistamines and anti-allergic compounds.
Plasma histamine and serum neutrophil chemotactic activity (S-NCA) were measured in ten atopic asthmatic patients on four separate occasions after allergen bronchial provocation testing (BPT). Single doses of inhaled sodium cromoglycate (SCG; 20 mg), clemastine (0.5 mg), ketotifen (0.5 mg) and isotonic saline (0.9% NaCl) placebo were administered 30 min before bronchial provocation testing in random order and double-blind. The airflow obstruction after BPT was monitored by measurement of forced expiratory volume in 1 s (FEV1). Plasma histamine was measured by the double-isotope radioenzymatic assay and S-NCA by a modified Boyden chamber technique. A highly significant decrease in FEV1 after BPT occurred on the placebo pre-treatment visit (P less than 0.001). Prior administration of inhaled SCG, clemastine and ketotifen significantly reduced the decrease in airflow obstruction seen after BPT when compared with placebo treatment (P less than 0.01, P less than 0.02, P less than 0.05 respectively). No significant alteration in plasma histamine was detected during allergen-induced airflow obstruction. Levels of S-NCA were significantly higher 5, 10 and 15 min after BPT when compared with the pre-challenge level (P less than 0.01, P less than 0.01, P less than 0.001 respectively). These levels were not significantly decreased when airflow obstruction was inhibited by the prior inhalation of SCG, clemastine or ketotifen.
Morgan
D J
DJ
Moodley
I
I
Cundell
D R
DR
Sheinman
B D
BD
Smart
W
W
Davies
R J
RJ
D000328:Adult; D000485:Allergens; D001249:Asthma / Q000188:drug therapy / Q000503:physiopathology*; D001985:Bronchial Provocation Tests / Q000379:methods; D002634:Chemotaxis, Leukocyte; D002974:Clemastine / Q000008:administration & dosage; D004205:Cromolyn Sodium / Q000008:administration & dosage; D004341:Drug Evaluation; D005260:Female; D005541:Forced Expiratory Volume; D006632:Histamine / Q000097:blood*; D006634:Histamine H1 Antagonists / Q000008:administration & dosage*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000188:drug therapy / Q000503:physiopathology; D007665:Ketotifen / Q000008:administration & dosage; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D013997:Time Factors
D000485:Allergens; D006634:Histamine H1 Antagonists; D006632:Histamine; D002974:Clemastine; D004205:Cromolyn Sodium; D007665:Ketotifen