Studies on the inhibitory effect of tetrandrine on silica-induced fibrogenesis in vitro.
Two cell culture systems composed of rat peritoneal macrophages and 2BS fibroblasts were used to study the inhibitory action of tetrandrine on collagen and glycosaminoglycan synthesis. The activation of silica-treated macrophage supernatants stimulated collagen synthesis by 2BS fibroblast cells. Tetrandrine had an inhibitory effect on the biosynthesis of collagen and glycosaminoglycans. The increase in the content of hydroxyproline, representing collagen, was verified by comparing it with that of DNA.
Li
Y R
YR
Section of Pneumoconiosis, Chinese Academy of Preventive Medicine, Beijing.
Cai
G P
GP
Liu
L F
LF
Chen
N M
NM
Li
C L
CL
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D044182:Benzylisoquinolines; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects*; D004247:DNA / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006909:Hydroxyproline / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D012822:Silicon Dioxide / Q000037:antagonists & inhibitors*
D000470:Alkaloids; D044182:Benzylisoquinolines; D006025:Glycosaminoglycans; C009438:tetrandrine; D012822:Silicon Dioxide; D003094:Collagen; D004247:DNA; D006909:Hydroxyproline