Detection of antibodies to bovine leukemia virus in bovine milk samples with an ELISA involving two monoclonal antibodies.
An indirect enzyme-linked immunosorbent assay was used to detect antibody to bovine leukemia virus (BLV). In the assay the gp51 antigen was selectively adsorbed to the solid phase using a monoclonal capture antibody. Specific antibodies in milk or serum samples were revealed by the addition of a monoclonal antibovine IgG 1 conjugated to peroxidase. All milk samples from animals found positive by serum testing were shown to be positive. BLV-positive milk samples were detected when added to pools of negative samples.
Florent
G
G
Research and Development Department, Norden-Europe, Louvain-la-Neuve, Belgium.
Delgoffe
J C
JC
Zygraich
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008892:Milk / Q000276:immunology*; D011237:Predictive Value of Tests; D012190:Retroviridae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral