Scrapie: how much do we really understand?
Biological studies have produced convincing evidence for different scrapie strains, some of which undergo mutation. This argues strongly in favour of the infectious scrapie agent having a genome. The length of incubation period is influenced by the strain of agent but is also under strict host control. In mice, this control is exerted by a gene called Sinc which affects the overall rate of agent replication in the CNS. After peripheral infection, invasion of the CNS from lymphoreticular sites of agent replication is a key step in pathogenesis. Evidence from one scrapie model indicates spread of infection along autonomic nerves to the thoracic spinal cord and then to other parts of the CNS. Other studies have shown that infection can spread in neurons. There are close relationships between the presence of replicating agent and the development of vacuolation, and also of cerebral amyloid when it occurs. We can, therefore, begin to understand the patterns of lesion development in the brain in terms of the targeting of infection and its replication at certain sites. Structures known as SAF (Scrapie Associated Fibrils) have been discovered in extracts of scrapie brain (but not uninfected brain) and a glycoprotein (PrP 27-30: SAF protein) is a major constituent of purified SAF. The glycoprotein is coded by a single gene which is present in several species and expressed in uninfected brain. The normal protein seems to be modified in scrapie infected brain so that it accumulates as SAF. The modified protein may also be deposited as extracellular amyloid because there appear to be common epitopes between SAF and scrapie amyloid. The biochemical nature of the scrapie agent remains in doubt and the association between infectivity and purified SAF may arise fortuitously from the fact that scrapie agent is 'sticky'.
Kimberlin
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D000682:Amyloid / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D018622:PrP 27-30 Protein; D011328:Prions / Q000235:genetics / Q000502:physiology*; D012608:Scrapie / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D014617:Vacuoles; D014779:Virus Replication
D000682:Amyloid; D009419:Nerve Tissue Proteins; D011328:Prions; D018622:PrP 27-30 Protein