A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone.
The cloned complementary DNA sequence encoding the human gonadotropin-releasing hormone (GnRH) precursor protein was used to construct an expression vector for the bacterial synthesis of the 56-amino acid GnRH-associated peptide (GAP). GAP was found to be a potent inhibitor of prolactin secretion and to stimulate the release of gonadotropins in rat pituitary cell cultures. Active immunization with peptides corresponding to GAP sequences led to greatly increased prolactin secretion in rabbits.
Nikolics
K
K
Mason
A J
AJ
Szönyi
E
E
Ramachandran
J
J
Seeburg
P H
PH
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007114:Immunization; D007986:Luteinizing Hormone / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D011388:Prolactin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011389:Prolactin Release-Inhibiting Factors / Q000502:physiology*; D011498:Protein Precursors / Q000235:genetics / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D012636:Secretory Rate
D011498:Protein Precursors; D007987:Gonadotropin-Releasing Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D004247:DNA; D011389:Prolactin Release-Inhibiting Factors