Detection of B-cell lymphoma in peripheral blood by DNA hybridisation.
As an alternative to morphological identification of circulating neoplastic lymphocytes in the blood of patients with non-Hodgkin's lymphoma, DNA of peripheral blood lymphocytes was analysed for clonal immunoglobulin gene rearrangements in 29 patients with low-grade B-cell lymphomas. 76% of the patients showed clonal rearrangements of immunoglobulin genes in their blood, including 58% of those with no other evidence of disease. In seven patients from whom paired samples were available the rearranged bands found in the blood and in lymph-node biopsy specimens containing histologically confirmed lymphoma were identical. Detection of circulating lymphoma cells by use of tumour-specific anti-idiotype antibodies and cytofluorimetry showed complete agreement with the results of DNA analysis.
Hu
E
E
Trela
M
M
Thompson
J
J
Lowder
J
J
Horning
S
S
Levy
R
R
Sklar
J
J
D001402:B-Lymphocytes; D003001:Cloning, Molecular; D004273:DNA, Neoplasm; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008223:Lymphoma / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D009693:Nucleic Acid Hybridization
D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains