Noradrenergic sprouting and beta-adrenergic receptor binding in the lateral geniculate nucleus of rats after unilateral visual cortex ablation.
One and two weeks after unilateral visual cortex (VC) ablation beta-adrenergic receptor binding is increased in the lateral geniculate nucleus (LGN) of both sides. 6 month later beta-receptor binding in the LGN is decreased again and no differences to untreated control animals are detectable. Using the glyoxylic acid fluorescence method for the visualization of amines a transient increase in the density of noradrenergic fibers in the dorsal part of the ipsilateral LGN can be demonstrated with a maximum 2 weeks after VC ablation. With longer survival time noradrenergic fibre density in the ipsilateral dorsal LGN (LGNd) decreases again and one year after the operation only few fluorescent fibres can be observed in the lateral part of the LGN compared to untreated control animals. Histologically an increased gliosis in the ipsilateral LGNd develops following removal of the visual cortex. In addition, degenerative changes in nerve fibers and terminals as well as neuronal degenerative changes are present and are most pronounced in the medial part of the ipsilateral dorsal LGN. Electron microscopically degenerating terminals in the dorsal part of the ipsilateral LGN can be identified as cortical afferents. Using potassium permanganate fixation typical noradrenergic axons with small dense core vesicles can be demonstrated in the LGN. In the denervated LGNd (i.e. the ipsilateral LGNd after visual cortex ablation) axon terminals with dense core vesicles appear exhibiting the structural peculiarities of growth cones seen during ontogenesis. They could be regarded as ultrastructural equivalent of the newly formed noradrenergic sprouts.(ABSTRACT TRUNCATED AT 250 WORDS)
Aurich
M
M
Paul Flechsig Institute for Brain Research, Department of Neurochemistry, Karl Marx University Leipzig, G.D.R.
Lüth
H J
HJ
Bigl
V
V
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D004292:Dominance, Cerebral / Q000502:physiology*; D005829:Geniculate Bodies / Q000033:anatomy & histology*; D008297:Male; D008854:Microscopy, Electron; D009410:Nerve Degeneration; D009416:Nerve Regeneration; D009473:Neuronal Plasticity; D009638:Norepinephrine / Q000502:physiology*; D011869:Radioligand Assay; D051381:Rats; D011941:Receptors, Adrenergic / Q000502:physiology*; D014793:Visual Cortex / Q000033:anatomy & histology*; D014795:Visual Pathways / Q000033:anatomy & histology*
D011941:Receptors, Adrenergic; D009638:Norepinephrine