title: The amino acid sequence of pancreatic spasmolytic polypeptide.
abstract: The sequence of porcine pancreatic spasmolytic polypeptide has been established by a variety of techniques including manual as well as automatic sequencing of fragments resulting from the cleavage of reduced and S-carboxymethylated pancreatic spasmolytic polypeptide with trypsin, chymotrypsin, clostripain, cyanogen bromide and formic acid. The N- and C-terminal sequences were established using pyroglutamate amino-peptidase and carboxypeptidase A, respectively. Pancreatic spasmolytic polypeptide contains 106 amino acid residues in a single chain with seven S-S bridges and a pyroglutamyl blocked N-terminal. The alignment of the sequences representing amino acids 14-49 and 63-98 shows pair-wise identical amino acid residues in 18 out of 36 positions, indicating that these two "domains" have been derived from a common gene.
authors.forename: L
authors.initials: L
authors.lastname: Thim
authors.forename: J
authors.initials: J
authors.lastname: Thomsen
authors.forename: M
authors.initials: M
authors.lastname: Christensen
authors.forename: K H
authors.initials: KH
authors.lastname: Jørgensen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism; D003488:Cyanogen Bromide; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000378:metabolism; D005561:Formates / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D011751:Pyroglutamyl-Peptidase I / Q000378:metabolism; D013552:Swine; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014357:Trypsin / Q000378:metabolism
chemical_list: D005561:Formates; D036341:Intercellular Signaling Peptides and Proteins; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010446:Peptide Fragments; D010455:Peptides; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; C030544:formic acid; C034407:pancreatic spasmolytic polypeptide; D010450:Endopeptidases; D011751:Pyroglutamyl-Peptidase I; D002918:Chymotrypsin; D014357:Trypsin; D003546:Cysteine Endopeptidases; C019913:clostripain; D003488:Cyanogen Bromide