title: Neuronal properties of monkey adrenal medulla in vitro.
abstract: Chromaffin cells from the monkey adrenal medulla were maintained in vitro in the presence of nerve growth factor (NGF) and the neuronal properties of these cells were assessed. Single-cell preparations were obtained by collagenase-trypsin treatment of the minced adrenal medulla tissue. Cells assumed a glandular to epithelioid morphology after twenty-four hours of culture. Twelve percent of these cells were shown to extend neurites spontaneously after five days. NGF-stimulated neuritic outgrowth from most cells after five days of culture and these neurites remained for at least three weeks. Cells exhibited intense histofluorescence for catecholamines even after three weeks in vitro in the presence of NGF and positive staining for tyrosine hydroxylase and dopamine beta hydroxylase could be detected by immunocytochemistry. Moreover, the chromaffin cells were shown to bind tetanus toxin, which is a specific marker for neurons. Tetanus toxin labelling was not dependent upon the presence of neurites on these cells. Transmission electron microscopy indicated that cultured cells contained numerous dense-core vesicles similar to non-cultured medulla cells. Many of the neurites possessed the morphological features of axons; long varicose processes resembling noradrenergic fibers were identified by catecholamine histofluorescence and tyrosine hydroxylase immunocytochemistry. Microtubular arrays, in an axonal-like organization pattern, were seen ultrastructurally along with the presence of many dense-core vesicles. These data support the potential of adult primate chromaffin cells as a source of sympathetic neuronal tissue for neural transplantation.
authors.forename: M F
authors.initials: MF
authors.lastname: Notter
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.forename: D M
authors.initials: DM
authors.lastname: Gash
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology*; D000375:Aging; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology; D002395:Catecholamines / Q000032:analysis; D002428:Cebus; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D003907:Dexamethasone / Q000494:pharmacology; D004299:Dopamine beta-Hydroxylase / Q000032:analysis; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D008854:Microscopy, Electron; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000201:enzymology / Q000502:physiology* / Q000637:transplantation / Q000648:ultrastructure; D013744:Tetanus Toxin / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D002395:Catecholamines; D009414:Nerve Growth Factors; D013744:Tetanus Toxin; D003907:Dexamethasone; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase