Restriction enzyme evidence for Alu sequence-mediated dispersion of microinjected genes in transgenic mice.
A human bacteriophage clone containing adult beta-globin genes with four Alu sequences was microinjected to produce transgenic mice. Southern blot analysis on the spleen of a transgenic mouse revealed an unusual hybridization pattern that suggested extensive dispersion of human DNA throughout the mouse genome. This pattern was reproducible using several restriction enzymes, including a noncutting enzyme. The hybridization pattern was not observed in other tissues, and sequences were not detected in progeny using the bacteriophage probe. However, hybridization of spleen DNA of offspring against a human Alu probe revealed genetic transmission of human Alu sequences. The results suggest dispersion of microinjected Alu sequences throughout the genome.
Rubinstein
W S
WS
Department of Biochemistry, Mt. Sinai School of Medicine, New York, NY 10029.
Gordon
J W
JW
D000818:Animals; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D005796:Genes; D005914:Globins / Q000235:genetics; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D012091:Repetitive Sequences, Nucleic Acid; D012880:Skin Pigmentation / Q000235:genetics
D004251:DNA Transposable Elements; D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes