title: Structural changes in DNA of human breast carcinoma.
abstract: Rearrangement and loss of genetic material in DNA of human ductal breast carcinoma was analyzed by restriction enzyme digestion, agarose gel electrophoresis and Southern blot hybridization using 32P labeled c-Ha-ras-1 and multilocus, minisatellite 15.1.11.4 as a probe. In several cases an allelic loss of c-Ha-ras-1 locus in heterozygous patients were noticed. Rearrangement of the genetic material in neoplastic tissues was also observed after hybridization with a minisatellite probe. These results provide evidence that loss and redistribution of the genetic material does occur in human ductal breast carcinoma.
authors.affiliation: Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Mackiewicz
authors.forename: B
authors.initials: B
authors.lastname: Choina
authors.forename: A
authors.initials: A
authors.lastname: Kadlof
authors.forename: S
authors.initials: S
authors.lastname: Szala
mesh_terms: D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000235:genetics*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics*; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D015321:Gene Rearrangement; D006801:Humans
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm