Biosynthesis of biotin-vitamers from unsaturated higher fatty acids by bacteria.
The biosynthesis of biotin-vitamers from unsaturated higher fatty acids by the resting cell system of bacteria was investigated. Biotin-vitamer formation from oleic, linoleic and linolenic acids was found in certain bacteria belonging to the genera Enterobacter, Agrobacterium, Bacillus, Escherichia and Micrococcus. The biotin-vitamers were identified as a mixture of 7,8-diaminopelargonic acid, desthiobiotin and biotin. Metabolites other than biotin-vitamers from those fatty acids were analyzed by gas-liquid chromatography and mass spectrometry. Linoleic, oleic, C-17 and C-15 saturated fatty acids were identified as the main metabolites of linolenic acid. The pathway of biosynthesis of pimelic acid from linolenic acid by a strain of Bacillus sphaericus AKU 0227 was also studied.
Ohsugi
M
M
Miyauchi
K
K
Inoue
Y
Y
D001407:Bacillus / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D001710:Biotin / Q000096:biosynthesis*; D002245:Carbon Dioxide / Q000378:metabolism; D002849:Chromatography, Gas; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D013058:Mass Spectrometry; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010101:Oxygen Consumption; D017962:alpha-Linolenic Acid
D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D008042:Linolenic Acids; D009829:Oleic Acids; D017962:alpha-Linolenic Acid; D002245:Carbon Dioxide; D019301:Oleic Acid; D001710:Biotin; D019787:Linoleic Acid