title: Determination of ectoenzyme activities in leukemic cells and in established hematopoietic cell lines.
abstract: Ectoenzyme activities were determined in peripheral blood cells from patients with acute leukemias, from normal controls, and in cells of hematopoietic cell lines. In common acute lymphoblastic leukemia, cell membrane-associated 5'-nucleotidase (5'-N) activity was significantly higher than in acute T and unclassified lymphoblastic leukemias. In acute myeloblastic and myelomonocytic leukemias, cells contained significantly higher gamma-glutamyl transpeptidase (gamma-GT) activity than in lymphoblastic leukemias. Normal B lymphocytes differed from T cells and monocytes mainly in their 5'-N activity, whereas in monocytes, gamma-GT activity was more pronounced than in other normal blood cells. Hematopoietic cell lines showed some distinct patterns of ectoenzyme activity. Most B cell lines had high 5'-N and (Na-K-Mg) adenosine triphosphatase activities. In lines of myeloid origin, elevated gamma-GT values were found. In lymphoid stem cells and in T lymphoblast lines, most ectoenzyme activities were lower than in the other cell lines. In some cell lines, characteristic high-activity marker enzymes were detected.
authors.forename: A
authors.initials: A
authors.lastname: Morell
authors.forename: G
authors.initials: G
authors.lastname: Losa
authors.forename: S
authors.initials: S
authors.lastname: Carrel
authors.forename: D
authors.initials: D
authors.lastname: Heumann
authors.forename: V E
authors.initials: VE
authors.lastname: von Fliedner
mesh_terms: D015720:5'-Nucleotidase; D000208:Acute Disease; D000251:Adenosine Triphosphatases / Q000032:analysis; D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D002460:Cell Line; D002648:Child; D006023:Glycoproteins / Q000032:analysis*; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D009708:Nucleotidases / Q000032:analysis; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000032:analysis; D005723:gamma-Glutamyltransferase / Q000032:analysis
chemical_list: D006023:Glycoproteins; D005723:gamma-Glutamyltransferase; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; D000251:Adenosine Triphosphatases