Usage of gamma chain variable regions in human acute lymphoblastic leukemias.
The purpose of the present study was to evaluate the use of the variable regions of the T gamma rearranging gene of the second T cell receptor. Genomic DNA was extracted from leukemic cells of T and B precursor phenotypes and hybridized to probes that detect rearrangement of the V gamma regions. We present data showing that the V gamma use is nonrandom in acute lymphoblastic leukemias (ALLs). B precursor ALL rearranged the more 5' variable regions. The results obtained suggest that the T precursor leukemias more frequently rearrange the 3' V gamma regions. Two leukemias that expressed the gamma protein rearranged V gamma 8 and V gamma 9.
González-Sarmiento
R
R
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.
Greenberg
J M
JM
Kersey
J H
JH
D002051:Burkitt Lymphoma / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D015336:Molecular Probe Techniques
D004273:DNA, Neoplasm