Comparison of the nucleotide sequences of diabetogenic and nondiabetogenic encephalomyocarditis virus.
The nucleotide sequences of a diabetogenic variant of encephalomyocarditis (EMC) virus (D variant, ifp- phenotype) and a nondiabetogenic variant of EMC virus (B variant, ifp+ phenotype) have been compared. These variants differ at eight sites. The poly(C) tract of EMC-B is three bases shorter than that of EMC-D. Of the seven sites at which nucleotide substitutions were confirmed using RNA templates, four resulted in amino acid changes in three different proteins, the leader peptide, 1B (VP2), and 1D (VP1). The biological significance of these differences can now be investigated.
Cohen
S H
SH
Department of Internal Medicine, University of California, School of Medicine, Davis 95616.
Naviaux
R K
RK
vanden Brink
K M
KM
Jordan
G W
GW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000382:microbiology; D004686:Encephalomyocarditis virus / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D010447:Peptide Hydrolases