Mapping of transmitter-specific connections: simultaneous demonstration of anterograde degeneration and changes in the immunostaining pattern induced by lesions.
This paper describes simple procedures which allow immunohistochemistry to be combined with a newly developed silver degeneration technique for the purpose of mapping transmitter-specific connections on the light microscopic level. Conditions of fixation, survival time and immunohistochemistry that contribute to optimal results are discussed.
Záborszky
L
L
Alheid
G F
GF
Heimer
L
L
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006652:Histological Techniques; D007124:Immunoenzyme Techniques; D009434:Neural Pathways / Q000033:anatomy & histology / Q000378:metabolism; D009445:Neuroanatomy / Q000379:methods*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D018377:Neurotransmitter Agents