The association of [3H]D-aspartate binding and high-affinity glutamate uptake in the human brain.
The binding of [3H]D-aspartate ([3H]D-Asp) to human cerebellum homogenate was compared with the uptake of [3H]glutamate ([3H]Glu) by homogenates prepared from rapidly frozen human cerebral cortex. There was a close correlation between the potencies of a range of drugs for inhibiting the binding of [3H]D-Asp and the uptake of [3H]L-Glu. Compounds selective for postsynaptic Glu receptors were inactive. The findings are consistent with the labelling of high-affinity Glu uptake sites by [3H]D-Asp, which may be a valuable ligand for studying excitatory amino acid terminals in human brain.
Cross
A J
AJ
Skan
W J
WJ
Slater
P
P
D001224:Aspartic Acid / Q000378:metabolism*; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006801:Humans; D008024:Ligands; D013237:Stereoisomerism
D005971:Glutamates; D008024:Ligands; D001224:Aspartic Acid; D018698:Glutamic Acid