title: Macrosorb Kieselguhr-agarose composite adsorbents. New tools for downstream process design and scale up. Scientific note.
abstract: Incompressible Macrosorb composite adsorbents, while retaining all the desirable properties of traditional agarose-based hydrogel media, overcome the operational limitations imposed by the use of soft hydrogels: They permit useful application of fast flow rates without restrictions on bed depth and they can be used in fluidized bed mode. Considerations which are important when contemplating scaled-up processing are discussed. A comparative cost estimate for a production process for extracting albumin from bovine serum in column equipment illustrates the various advantages which may be exploited when using a composite adsorbent in place of a conventional soft gel equivalent.
authors.affiliation: Sterling Organics R&D, Newcastle upon Tyne, England.
authors.forename: M G
authors.initials: MG
authors.lastname: Bite
authors.forename: S
authors.initials: S
authors.lastname: Berezenko
authors.forename: F J
authors.initials: FJ
authors.lastname: Reed
authors.forename: L
authors.initials: L
authors.lastname: Derry
mesh_terms: D000327:Adsorption; D007692:Diatomaceous Earth; D012685:Sepharose; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D012822:Silicon Dioxide
chemical_list: D012710:Serum Albumin, Bovine; D007692:Diatomaceous Earth; D012822:Silicon Dioxide; D012685:Sepharose