title: Comparative studies of rodent anionic arylsulfatases.
abstract: Approximately 25 and 40%, respectively, of murine (Mus musculus) and rat (Rattus norvegicus) hepatic arylsulfatase (EC 3.1.6.1) activity eluted from DEAE-ion exchange resins under high salt conditions. This high salt fraction contained arylsulfatase A and an enzyme which was immunologically similar to arylsulfatase B. The latter enzyme was thermostable, resistant to inhibition by silver, completely inhibited by phosphate, displayed linear kinetics, and had a higher pH optimum than arylsulfatase A. Anionic arylsulfatase B also hydrolyzed chondroitin-4-SO4 heptasaccharide. Sephacryl S-300 gel filtration resolved anionic arylsulfatase B into 55 and 115 kd fractions. Rodent arylsulfatase A activity was grossly underestimated when 4-methyl-umbelliferyl sulfate was employed as substrate.
authors.forename: D B
authors.initials: DB
authors.lastname: Thompson
authors.forename: W L
authors.initials: WL
authors.lastname: Daniel
authors.forename: J H
authors.initials: JH
authors.lastname: Glaser
mesh_terms: D000818:Animals; D002417:Cattle; D002553:Cerebroside-Sulfatase / Q000302:isolation & purification / Q000378:metabolism*; D002803:Chondro-4-Sulfatase / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D051379:Mice; D009439:Neuraminidase; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013429:Sulfatases / Q000378:metabolism*
chemical_list: D013429:Sulfatases; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase; D009439:Neuraminidase