In vitro-derived leukemic erythroid cell lines induced by a raf- and myc-containing retrovirus differentiate in response to erythropoietin.
In vitro infection of murine fetal liver cells with a retrovirus containing v-raf and v-myc oncogenes has produced continuous lines of immature erythroid cells that are leukemogenic. These cells synthesized a factor that stimulated their growth in vitro before autonomous variants emerged. Approximately 1000 high-affinity erythropoietin receptors could be detected per cell, and the hormone induced terminal differentiation in these cells. The lines were generated at an extremely low frequency (approximately 1 in 10(7) cells), suggesting that the combination of raf and myc is insufficient to develop erythroid cell lines and that additional events are necessary for transformation.
Klinken
S P
SP
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Nicola
N A
NA
Johnson
G R
GR
D000011:Abelson murine leukemia virus / Q000235:genetics*; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004921:Erythropoietin / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000382:microbiology; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D008980:Moloney murine sarcoma virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011956:Receptors, Cell Surface / Q000502:physiology*; D017467:Receptors, Erythropoietin; D009053:Sarcoma Viruses, Murine / Q000235:genetics*
D000954:Antigens, Surface; D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D004921:Erythropoietin