HPLC purification of the natural ATPase inhibitor from the yeast Candida utilis.
A rapid method of preparation of the natural ATPase inhibitor (IF1) from the mitochondria of the yeast Candida utilis has been developed. It involved high performance liquid chromatography (HPLC) as the final step of purification. An active form of Candida utilis IF1 was obtained free of contaminant. Its properties are compared with those of IF1 from other sources.
Dianoux
A C
AC
D000818:Animals; D002175:Candida / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D000097795:ATPase Inhibitory Protein
D011506:Proteins; D006180:Proton-Translocating ATPases; D000097795:ATPase Inhibitory Protein