title: A tumor necrosis factor binding protein is present in human biological fluids.
abstract: Tumor necrosis factor (TNF) possesses both beneficial and toxic bioactivities. Mechanisms may operate to counteract harmful effects. We have identified a TNF binding protein (TNF-BP), which shows increased levels in serum and urine of patients on regular hemodialysis treatment (RDT). TNF-BP inhibited the specific binding of human recombinant TNF (rTNF) to its cell surface receptor. Results from gel chromatography demonstrated the presence in serum and urine of a macromolecule with an apparent molecular weight of 50,000, which formed a complex with rTNF. A 62-fold purification of TNF-BP from urine of patients on RDT was achieved by ion exchange chromatography and gel chromatography. Partially purified TNF-BP reduced the growth inhibitory effect of rTNF on a susceptible leukemia cell line. TNF-BP may act as a regulator of the biological activity of TNF and could have beneficial effects in certain inflammatory conditions.
authors.affiliation: Department of Medicine, University of Lund, Malmö, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Peetre
authors.forename: H
authors.initials: H
authors.lastname: Thysell
authors.forename: A
authors.initials: A
authors.lastname: Grubb
authors.forename: I
authors.initials: I
authors.lastname: Olsson
mesh_terms: D001667:Binding, Competitive; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D007674:Kidney Diseases / Q000097:blood / Q000652:urine; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D006435:Renal Dialysis; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha