[Thin-layer densitometric determination of clotiazepam in tablets and blood plasma].
Quantitative determination of 7-ethyl-5-(2-chlorophenyl)-1-methyl-1,3-dihydro-2H-thieno[2,3-e]1, 4-diazepin-2-one (clotiazepam, Trecalmo) as pure substance and after extraction from tablets can be carried out by high performance thin-layer densitometry in absorbance at 243 nm. Detection limit was 10 ng/spot, and inaccuracy +/- 1.8% in the microgram range. For determination of clotiazepam in blood plasma, a more sensitive method was developed by fluorodensitometry above 460 nm after excitation at 313 nm. Analytical parameters are: detection limit 2.5 ng/ml, inaccuracy between 25 and 300 ng/ml +/- 5%, and imprecision at a concentration of 100 ng/ml +/- 5%. Plasma level determination up to 12 samples can be achieved in about 5 h. Thus, for determination of only a few samples, the HPTLC method is less time-consuming than the gas chromatographic assay.
Busch
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Ritter
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Möhrle
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H
D000818:Animals; D001381:Azepines / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D002417:Cattle; D003720:Densitometry / Q000379:methods; D006801:Humans; D013050:Spectrometry, Fluorescence / Q000379:methods; D013607:Tablets
D001381:Azepines; D013607:Tablets
Dünnschicht-densitometrische Bestimmung von Clotiazepam in Tabletten und in Blutplasma.