title: In vitro cytotoxicity of chrysotile asbestos to human pulmonary alveolar macrophages is decreased by organosilane coating and surfactant.
abstract: Human pulmonary alveolar macrophages were used to quantitate the cytotoxic effect of surface-altered chrysotile asbestos. Little difference was observed in mortality between chrysotile asbestos that was surface-treated to a 42% extent by a hydrophobic organosilane or untreated chrysotile. Little or no effect on mortality was observed when human pulmonary alveolar macrophages were cultured with untreated chrysotile or acid-leached asbestos in the presence of 10 mM dipalmitoyl lecithin. However, when human pulmonary alveolar macrophages were cultured with a hydrophobically-treated (to a 42% or 95% extent) chrysotile asbestos in the presence of 10 mM dipalmitoyl lecithin, a statistically significant decrease in mortality was observed compared to untreated chrysotile. No mutagenic activity was observed when V79 cells were cultured with acid-leached, or 42% hydrophobically-treated chrysotile asbestos, even when human pulmonary alveolar macrophages were included as an activation source. The 95% hydrophobically-treated and acid-leached chrysotile also exhibited decreased binding of benzo[a]pyrene compared to untreated chrysotile asbestos.
authors.affiliation: Baylor College of Medicine, Houston, Texas.
authors.forename: D G
authors.initials: DG
authors.lastname: Morrison
authors.forename: T L
authors.initials: TL
authors.lastname: McLemore
authors.forename: E C
authors.initials: EC
authors.lastname: Lawrence
authors.forename: D G
authors.initials: DG
authors.lastname: Feuerbacher
authors.forename: M L
authors.initials: ML
authors.lastname: Mace
authors.forename: D L
authors.initials: DL
authors.lastname: Busbee
authors.forename: A C
authors.initials: AC
authors.lastname: Griffin
authors.forename: M V
authors.initials: MV
authors.lastname: Marshall
mesh_terms: D000328:Adult; D001194:Asbestos / Q000633:toxicity*; D017632:Asbestos, Serpentine; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D008297:Male; D009152:Mutagenicity Tests; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D017632:Asbestos, Serpentine; D013501:Surface-Active Agents; D001194:Asbestos