title: [Isolation and various properties of nucleoside monophosphate kinases from Escherichia coli].
abstract: A technique is proposed for isolation of nucleosidemonophosphate kinases--AMP-kinase (EC 2.7.4.11), GMP-kinase (EC 2.7.4.8), CMP-kinase (EC 2.7.4.14), UMP-kinase (EC 2.7.4.14) and TMP-kinase (EC 2.7.4.9)--from E. coli MRE-600. It involves cell destroying, precipitation of nucleic acids with polyethyleneimine, fractionation with ammonium sulphate followed by chromatography on different carriers (DEAE-Toyopearl-650 M, Matrex gel Blue A, Matrex gel Red A). The technique enables all the five enzymes to be obtained separately and without contaminations with nucleotide dephosphorylating enzymes. For all the enzymes the pH optimum was found to range from 6.5 to 8.0, and Mg2+ ions were found to be the best activator for all the enzymes studied. The substrate specificity was investigated with respect to acceptors and donors of the phosphate groups. The enzymes showed strict specificity to the heterocyclic base of the acceptor phosphate group. AMP-, GMP- and CMP-kinases phosphorylated the corresponding deoxynucleoside monophosphates less effectively than ribonucleoside monophosphates. ATP was found to be the most effective phosphate donor for all the enzymes under study.
authors.forename: V A
authors.initials: VA
authors.lastname: Rikhter
authors.forename: I V
authors.initials: IV
authors.lastname: Rabinov
authors.forename: S A
authors.initials: SA
authors.lastname: Kuznetsov
authors.forename: T B
authors.initials: TB
authors.lastname: Pavliĭ
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Skoblov
mesh_terms: D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D004926:Escherichia coli / Q000201:enzymology*; D009703:Nucleoside-Phosphate Kinase / Q000032:analysis / Q000302:isolation & purification*; D010770:Phosphotransferases / Q000302:isolation & purification*; D013379:Substrate Specificity
chemical_list: D010770:Phosphotransferases; D009703:Nucleoside-Phosphate Kinase
vernacular_title: Vydelenie i nekotorye svoĭstva nukleozidmonofosfatkinaz iz Escherichia coli.