title: The effect of growth conditions on adenylate cyclase activity and virulence-related properties of Bordetella pertussis.
abstract: Growth of Bordetella pertussis in Stainer & Scholte medium in which the NaCl had been replaced by one of several inorganic or organic salts resulted in a large decrease in adenylate cyclase activity, histamine-sensitizing activity and in the amounts of two cell-envelope polypeptides of Mr 28000 and 30000. Although some variation between strains was observed, there was never a case where one of these properties was lost independently of the others. Cultures in which these properties were lost had decreased amounts of extracellular cAMP when compared to NaCl-grown cultures. Adenylate cyclase activity was detected in three locations of B. pertussis cultures (extracellular, extracytoplasmic but cell-associated, and cytoplasmic). After growth in medium containing high concentrations of MgSO4, enzyme activity was decreased to a similar extent in all three locations.
authors.forename: R M
authors.initials: RM
authors.lastname: Brownlie
authors.forename: R
authors.initials: R
authors.lastname: Parton
authors.forename: J G
authors.initials: JG
authors.lastname: Coote
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis; D001886:Bordetella pertussis / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D007223:Infant; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D000242:Cyclic AMP; D000262:Adenylyl Cyclases