Superoxide anion measurement by sulfonated phenyl isothiocyanate cytochrome c.
Sulfonated phenyl isothiocyanate cytochrome c is suggested as a new scavenger for superoxide anion. The efficiency of the modified cytochrome c in measurements is compared with that of phenyl isothiocyanate cytochrome c and acetylated cytochrome c. Sulfonated phenyl isothiocyanate cytochrome c is water and salt soluble. Autooxidability of the pigment is not observed. The primary advantage of sulfonate phenyl isothiocyanate cytochrome c is that it appears to be specifically reduced by O2 radicals without interferences by other reactions in complex biological systems.
Morel
F
F
Département de Recherche Fondamentale, Centre d'Etudes Nucléaires, Grenoble, France.
Dianoux
A C
AC
Vignais
P V
PV
D000107:Acetylation; D002848:Chromatography, DEAE-Cellulose; D003574:Cytochrome c Group / Q000138:chemical synthesis*; D017879:Isothiocyanates; D010084:Oxidation-Reduction; D013481:Superoxides / Q000032:analysis*; D013861:Thiocyanates / Q000138:chemical synthesis*
D003574:Cytochrome c Group; D017879:Isothiocyanates; D013861:Thiocyanates; C005441:phenylisothiocyanate; D013481:Superoxides