Application of the peroxidase-antiperoxidase technique for the detection of enterotoxigenic Escherichia coli K99 pilus antigen in paraffin-embedded tissues.
The peroxidase-antiperoxidase (PAP) technique was evaluated as a method for detecting enterotoxigenic Escherichia coli (ETEC) K99 pilus antigen in paraffin-embedded tissues of swine. Antigenic reactivity was optimal when tissues were processed in cold methanol according to a modified method of Sainte-Marie. This method of processing adequately preserved tissue morphology. The PAP technique was applicable to tissues where endogenous peroxidase activity was minimal.
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D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000276:immunology*; D010861:Fimbriae, Bacterial / Q000276:immunology*; D005557:Formaldehyde; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008722:Methods; D008854:Microscopy, Electron; D010232:Paraffin; D013552:Swine
D000942:Antigens, Bacterial; D005557:Formaldehyde; D010232:Paraffin