title: Ecology of the murine alphaherpesvirus and its isolation from lungs of rodents in cell culture.
abstract: Together 381 sera of murine rodents (Apodemus flavicollis, Clethrionomys glareolus, Microtus arvalis and Mus musculus) trapped in different localities of Czechoslovakia were examined for the presence of antibodies to a murine alphaherpesvirus (MHV) and to murine cytomegalovirus (CMV). Positivity of rodent sera to the two MHV and one murine CMV strains varied from none to 12.5% and from none up to 11.3%, respectively, depending on the locality under study. From the lungs of A. flavicollis showing serum antibodies to MHV, a further murine herpes virus strain was isolated in rabbit embryo fibroblasts (REF). The latter MHV reached a titre of 10(10) TCID50/ml in the 13th passage, causing typical cytopathic effect from the 2nd passage on.
authors.forename: J
authors.initials: J
authors.lastname: Mistríková
authors.forename: D
authors.initials: D
authors.lastname: Blaskovic
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D003411:Arvicolinae; D002460:Cell Line; D003587:Cytomegalovirus / Q000302:isolation & purification; D003604:Czechoslovakia; D006564:Herpesviridae / Q000276:immunology / Q000302:isolation & purification*; D008168:Lung / Q000382:microbiology*; D051379:Mice; D009115:Muridae; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral