A superfusion apparatus for the examination of neurotransmitter release from synaptosomes.
A superfusion apparatus for examining the release of neurotransmitter from synaptosomes is described. The apparatus provides for accurate temperature control, rapid switching and continuous gassing of superfusing buffers, short (i.e. 1-s) pulses of high-potassium-containing buffer for examining depolarized release, rapid collection of superfusing eluate, and low tissue chamber dead volume. Depolarization (high-potassium) evoked release and to some degree spontaneous release of [3H]dopamine from rat striatal synaptosomes are dependent on buffer calcium concentration. The putative calcium channel blocker nickel reduces both spontaneous and high-potassium-evoked [3H]dopamine release.
Minnema
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D
Michaelson
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IA
D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D006531:HEPES; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009994:Osmolar Concentration; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*
D018377:Neurotransmitter Agents; D011188:Potassium; D006531:HEPES; D004298:Dopamine