title: Viral and cellular deoxyribonucleases are associated with herpes simplex virus replicative intermediates.
abstract: The presence of viral and cellular deoxyribonucleases was investigated in partially purified HSV replicative intermediates (nucleoprotein complexes) and nucelocapsids prepared from BHK-21 cell cultures infected with herpes simplex virus type 1. The 85kd virus-coded alkaline nuclease was detected by Western blotting experiments in infected cell extracts 7 h after infection, and was identified in viral DNA containing nucleoprotein complexes at 18 h after infection. With activity gel experiments, three cellular deoxyribonucleases were detected in mock-infected cells. Two of them were present in infected cell extracts, and one of them (50kd) was detected in nucleocapsids at 9 h P.I., and in nucleoprotein complexes at 18 h P.I. These data indicate a possible role of viral nuclease in the biosynthesis of late replicative intermediates, and suggest an active involvement of the nuclear matrix in viral replication.
authors.affiliation: Istituto di Scienze Biologiche, Università di Verona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Turco
authors.forename: P F
authors.initials: PF
authors.lastname: Pignatti
mesh_terms: D000818:Animals; D001345:Autoradiography; D015153:Blotting, Western; D002213:Capsid / Q000032:analysis; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000032:analysis; D003851:Deoxyribonucleases / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D009698:Nucleoproteins / Q000032:analysis*; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D014758:Viral Core Proteins / Q000032:analysis; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D009698:Nucleoproteins; D014758:Viral Core Proteins; D003851:Deoxyribonucleases