Surface marker characterization of EBV-carrying cells in blood of healthy seropositive individuals.
We have determined the phenotype of EBV-carrying cells in human blood by establishing spontaneous LCLs from populations selected according to three B cell markers, B1, B2, and 35.1C5. LCLs grew from the B1-positive, B2-positive, B2-negative and 35.1C5-positive population. Thus, EBV-carrying cells in blood of seropositive individuals are restricted to the B cell lineage. These B cells may or may not express the B2 epitope, and they may be derived from the lymph node mantle zone or the splenic marginal zone. These in vivo EBV-carrying cells could enter the viral productive cycle and transform coresident B cells during the initial 3 days in vitro. The characteristic of the LCLs with regard to these 3 B cell markers did not correspond to the original B cell population from which they were derived.
Lewin
N
N
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Aman
P
P
Wennborg
A
A
Ehlin-Henriksson
B
B
Manneborg-Sandlund
A
A
Klein
E
E
Klein
G
G
D000328:Adult; D000914:Antibodies, Viral / Q000302:isolation & purification*; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000276:immunology / Q000382:microbiology*; D015415:Biomarkers; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans
D000914:Antibodies, Viral; D000954:Antigens, Surface; D015415:Biomarkers