title: A comparison of bovine growth-hormone gene expression in mouse L cells directed by the Moloney murine-leukemia virus long terminal repeat, simian virus-40 early promoter or cytomegalovirus immediate-early promoter.
abstract: Three transcriptional regulatory regions including a human cytomegalovirus immediate-early region (CMVIE) promoter, the simian virus-40 early region (SV40E) promoter, and Moloney murine leukemia virus (MoMLV) long terminal repeat (LTR) were ligated to the bovine growth hormone (bGH)-coding gene. Using a bGH transient expression system, the CMVIE and SV40E gene transcriptional regulatory regions were found to be approximately two-fold more efficient than the MoMLV LTR in directing expression of bGH in mouse L cells.
authors.affiliation: Department of Zoology and Biomedical Sciences, Edison Animal Biotechnology Center, Athens, OH.
authors.forename: A
authors.initials: A
authors.lastname: Martin-Gallardo
authors.forename: M
authors.initials: M
authors.lastname: Montoya-Zavala
authors.forename: B
authors.initials: B
authors.lastname: Kelder
authors.forename: J
authors.initials: J
authors.lastname: Taylor
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: F C
authors.initials: FC
authors.lastname: Leung
authors.forename: J J
authors.initials: JJ
authors.lastname: Kopchick
mesh_terms: D000818:Animals; D002417:Cattle; D003587:Cytomegalovirus / Q000235:genetics; D004274:DNA, Recombinant; D005809:Genes, Regulator; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics*; D007739:L Cells; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
chemical_list: D004274:DNA, Recombinant; D013006:Growth Hormone