Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei.
Trypanosoma brucei variant surface glycoprotein (VSG) genes are activated either by duplicative (DA) transposition of the gene to a pre-activated expression site or by nonduplicative (NDA) activation of a previously silent telomeric gene. We have obtained a recombinant clone spanning the 5' barren region of the expression linked copy of the duplicated VSG gene 117a. By DNA sequence and hybridization analyses we have identified a pleomorphic family of 14-25 non-VSG genes that lie upstream of both DA and NDA VSG expression sites. These expression site associated genes (ESAGs) encode 1.2 kb poly(A)+ mRNAs that are specifically transcribed from the active VSG expression telomere in mammalian bloodstream stages of T. brucei but, in common with VSG genes, are not transcribed in procyclic culture forms. cDNA and genomic sequences predict open reading frames that are conserved in the two ESAGs examined.
Cully
D F
DF
Ip
H S
HS
Cross
G A
GA
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D014643:Variant Surface Glycoproteins, Trypanosoma
D004274:DNA, Recombinant; D006023:Glycoproteins; D012333:RNA, Messenger; D014643:Variant Surface Glycoproteins, Trypanosoma; D011061:Poly A; D004247:DNA