title: Reduced DNA repair response of cells from carcinogen-induced rat liver altered foci exposed to chemical carcinogens in culture.
abstract: Cells were cultured from rat livers containing enzyme-altered foci induced by N-2-fluorenylacetamide and the level of DNA repair synthesis in response to several carcinogens was compared in gamma-glutamyl transpeptidase-positive foci cells and in enzyme-negative hepatocytes from the same livers. The level of unscheduled DNA synthesis elicited in foci cells by either the activation-dependent carcinogens N-2-fluorenylacetamide and diethylnitrosamine or the activation-independent carcinogens N-methyl-N'-nitro-N-nitrosoguanidine and 1-methyl-1-nitrosourea was significantly lower than that in hepatocytes. The results suggest that the repair of DNA damage by these altered cells is abnormal.
authors.forename: H
authors.initials: H
authors.lastname: Mori
authors.forename: S
authors.initials: S
authors.lastname: Sugie
authors.forename: F
authors.initials: F
authors.lastname: Ohbayashi
authors.forename: H
authors.initials: H
authors.lastname: Shima
authors.forename: N
authors.initials: N
authors.lastname: Yoshimi
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: G M
authors.initials: GM
authors.lastname: Williams
mesh_terms: D015073:2-Acetylaminofluorene; D000818:Animals; D002273:Carcinogens; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008769:Methylnitronitrosoguanidine; D051381:Rats; D011912:Rats, Inbred ACI; D005723:gamma-Glutamyltransferase / Q000032:analysis*
chemical_list: D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D015073:2-Acetylaminofluorene; D005723:gamma-Glutamyltransferase