A radial immunodiffusion enzyme assay for detection of antibody to equine rhinopneumonitis virus (EHV-1) in horse serum.
A radial immunodiffusion enzyme assay (RIDEA) was developed for detection and quantitation of antibodies to equine herpes virus-1 (EHV-1) in horse sera. The detection and quantitation of EHV-1 antibody levels were based on the diameter of the radial diffusion zone of specific antibody in each serum sample reacting with EHV-1 antigen. The circular zone was made visible using peroxidase-conjugated rabbit anti-horse immunoglobulin G and a substrate containing hydrogen peroxide. The results of the RIDEA were compared with those of virus neutralization (VN) and enzyme-linked immunosorbent assay (ELISA) and found to be highly correlated. The relative sensitivity and specificity (percentage of agreement with VN test) were found to be 98.2 and 92.5%, respectively. Because the test procedure is relatively easy to perform, the RIDEA could be used as a field test to detect antibodies to EHV-1 in horses.
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Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Joo
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D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006564:Herpesviridae / Q000276:immunology*; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D011237:Predictive Value of Tests
D000914:Antibodies, Viral