A glycophospholipid tail at the carboxyl terminus of the Thy-1 glycoprotein of neurons and thymocytes.
Cell surface molecules of eukaryotic cells have been considered to be integrated into the membrane bilayer by a transmembrane protein sequence. The Thy-1 antigen of rodent thymocytes and brain was the first eukaryotic membrane molecule for which biochemical data clearly suggested membrane integration via a nonprotein tail. Direct evidence is now presented showing that a glycophospholipid structure is attached to the carboxyl-terminal cysteine residue and that 31 carboxyl-terminal amino acids predicted from the Thy-1 complementary DNA sequence are not present in the mature glycoprotein. These experimental results raise questions concerning signaling across a cell membrane since antibodies to Thy-1 can stimulate T lymphocytes to release lymphokines and undergo cell division.
Tse
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Barclay
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Watts
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Williams
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D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000302:isolation & purification; D001921:Brain / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism; D005688:Galactosamine / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D013229:Stearic Acids / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D004983:Ethanolamines; D006017:Glycolipids; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D013229:Stearic Acids; D018800:Thy-1 Antigens; C031183:stearic acid; D005688:Galactosamine; D005944:Glucosamine