Organization of the gene encoding the insulin-like growth factor binding protein IBP-1.
Human genomic clones encompassing the tissue specific expressed gene IBP-1, an insulin-like growth factor binding protein were isolated and characterized. The gene is organized in four exons and spans 5.9 kb. S1 nuclease analysis determined a single transcription start site. The first exon and 5' flanking region are highly GC rich and located in a CpG island. The CpG island enclose the CAAT box, the TATA box, the transcription start site and a potential SP1 transcription factor binding site. The presumptive promoter region is characteristic for genes expressed in a tissue specific fashion. All signals required for cleavage/polyadenylation are located within exon IV, predicting a mRNA of 1.5 kb which is consistent with the size seen on RNA blots.
Brinkman
A
A
Department of Pediatrics, Erasmus University/Sophia Childrens Hospital, Rotterdam, The Netherlands.
Groffen
C A
CA
Kortleve
D J
DJ
Drop
S L
SL
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015342:DNA Probes; D004720:Endonucleases; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D011972:Receptor, Insulin / Q000235:genetics*; D017451:Receptors, Somatomedin; D012045:Regulatory Sequences, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D015342:DNA Probes; D017451:Receptors, Somatomedin; D004247:DNA; D011972:Receptor, Insulin; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases