Direct evidence for the presence of GABAA receptors on the cytoplasmic side of the Deiters' neurone membrane.
A newly developed micromethod has been used for studying the rate of passage of 36Cl- ions across single nerve membranes from rabbit Deiters' neurones. The application of gamma-aminobutyric acid (GABA) on the cytoplasmic side of those membranes increases the rate of passage of 36Cl- ions from that side to the other one across the membrane. The maximal effect is exerted by 10(-6) M GABA and it fades at higher neurotransmitter concentrations (10(-5) M to 3.3 x 10(-3) M). The cause of this fading of the effect appears to be a receptor desensitization phenomenon. The 10(-6) M GABA effect is reversed by both 10(-4) M picrotoxin and 10(-5) M bicuculline. The overall pattern of the data indicates the presence of GABAA receptors on the cytoplasmic side of these nerve membranes.
Rapallino
M V
MV
Centro di Neurofisiologia Cerebrale, C.N.R., Genova, Italy.
Cupello
A
A
Hydén
H
H
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002712:Chlorides / Q000493:pharmacokinetics; D003593:Cytoplasm / Q000378:metabolism*; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010852:Picrotoxin / Q000494:pharmacology; D011817:Rabbits; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D001640:Bicuculline; D002712:Chlorides; D010852:Picrotoxin; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid