title: Hepatic glutathione S-transferase activity and aflatoxin B1-induced enzyme altered foci in rats fed fractions of brussels sprouts.
abstract: The aim of the present study was to determine whether the liver cytosol detoxication enzymes, glutathione S-transferases (GSTases) as well as gamma-glutamyl transpeptidase (GGT) foci induced by aflatoxin B1 (AFB) were changed by feeding weanling rats diets containing brussels sprouts, a glucosinolate fraction of brussels sprouts (extract), or a non-glucosinolate fraction (residue). All 3 of these diets induced high levels of hepatic GSTase specific activity as compared to purified-basal diet fed control rats. The brussels sprouts and the extract treatments, but not the residue dietary treatment, inhibited hepatic GGT foci induced by AFB. Thus, glucosinolates and non-glucosinolate fractions of brussels sprouts induce hepatic enzymes involved in detoxication mechanisms but the non-glucosinolate compound(s) apparently are not involved in all chemical carcinogen metabolic processes.
authors.forename: C E
authors.initials: CE
authors.lastname: Godlewski
authors.forename: J N
authors.initials: JN
authors.lastname: Boyd
authors.forename: W K
authors.initials: WK
authors.lastname: Sherman
authors.forename: J L
authors.initials: JL
authors.lastname: Anderson
authors.forename: G S
authors.initials: GS
authors.lastname: Stoewsand
mesh_terms: D016604:Aflatoxin B1; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D001937:Brassica; D005982:Glutathione Transferase / Q000032:analysis*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D005723:gamma-Glutamyltransferase / Q000032:analysis*
chemical_list: D000348:Aflatoxins; D016604:Aflatoxin B1; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase