title: Comparison of immunohistochemical staining of a mitochondrial protein, lipoamide dehydrogenase, with Fab'-peroxidase conjugates prepared by maleimide or periodate.
abstract: IgG-maleimide peroxidase, Fab'-maleimide peroxidase, polymer and monomer types of Fab'-periodate peroxidase were prepared from an antibody against rat lipoamide dehydrogenase, a component of the pyruvate dehydrogenase complex which is located in mitochondria. They were examined for immunohistochemical staining of the rat kidney. Fab'-maleimide peroxidase was the best for staining mitochondrial protein. IgG-maleimide peroxidase and the monomer type of Fab'-periodate peroxidase had the same intensity of staining. The polymer type of Fab'-periodate peroxidase could not stain the lipoamide dehydrogenase.
authors.affiliation: Department of Biochemistry, School of Medicine, Kagoshima University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matuda
authors.forename: Y
authors.initials: Y
authors.lastname: Yagi
authors.forename: T
authors.initials: T
authors.lastname: Saheki
authors.forename: S
authors.initials: S
authors.lastname: Hashida
authors.forename: E
authors.initials: E
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007668:Kidney / Q000201:enzymology*; D008301:Maleimides; D010504:Periodic Acid; D010544:Peroxidases; D051381:Rats
chemical_list: D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D008301:Maleimides; D010504:Periodic Acid; C043592:maleimide; D010544:Peroxidases; D008058:Dihydrolipoamide Dehydrogenase