Evidence that Lactobacillus casei insertion element ISL1 has a narrow host range.
The 1.3-kilobase-pair insertion element ISL1, originally isolated from Lactobacillus casei S-1, was found to have an extremely restricted host range. By DNA-DNA hybrizations performed with Southern transfers by using a cloned internal fragment of ISL1 as a molecular probe, it was found that only 3 of 19 L. casei strains examined contained sequences that hybridized to the ISL1 probe. In two of these, the hybridizing sequences were found on lactose plasmids. No homologous sequences were detected in a survey of 14 other Lactobacillus strains (9 species) and 15 strains of other bacteria (8 genera, 12 species).
Shimizu-Kadota
M
M
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
Flickinger
J L
JL
Chassy
B M
BM
D015139:Blotting, Southern; D004251:DNA Transposable Elements; D007780:Lacticaseibacillus casei / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004251:DNA Transposable Elements