Detection of antibodies against porcine parvovirus in swine sera by enzyme-linked immunosorbent assay.
An enzyme-linked immunosorbent assay (ELISA) was developed for detection of antibody against porcine parvovirus in swine sera. The antigen used for the assay was partially-purified virus treated with fluorocarbon and shown to contain 7 proteins by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Of these proteins 83-, 64- and 60-K proteins reacted in Western immunoblotting with swine serum after infection with porcine parvovirus. Antibody responses were demonstrated by ELISA in pigs subcutaneously-infected with porcine parvovirus as by hemagglutination-inhibition (HI) test and Western immunoblotting reaction with the 83-, 64- and 60-K viral proteins. The results of ELISA on random swine-serum samples were well-correlated with those of the HI test. These findings indicate the usefulness of the ELISA as a serological tool for porcine parvovirus infection.
Hohdatsu
T
T
Research Center for Veterinary Science, Kitasato Institute, Chiba-Ken, Japan.
Baba
K
K
Ide
S
S
Tsuchimoto
M
M
Nagano
H
H
Yamagami
T
T
Yamagishi
H
H
Fujisaki
Y
Y
Matumoto
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D009500:Neutralization Tests; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000276:immunology / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral