title: Interaction of different nucleotides with Ca-release channels from heavy sarcoplasmic reticulum.
abstract: Calcium accumulation and release from the heavy fraction of sarcoplasmic reticulum vesicles have been studied in the presence of different nucleotides with the use of the Ca-sensitive dye antipyrylazo III for monitoring of the free Ca2+ concentration. The calcium- and caffeine-induced Ca2+ release is observed only with ATP but not with any of nonadenine nucleotides used as substrates for the Ca-pump. Adenine nucleotides provide for a rapid ruthenium red sensitive Ca2+ release from the vesicles, when nonadenine nucleotides are used as energy sources for Ca2+ uptake. A comparison of the nucleotides interaction with Ca-channels and Ca-ATPase supports the hypothesis that Ca-ATPase is involved in the operation of Ca-channels.
authors.affiliation: Department of Biochemistry, Moscow State University, U.S.S.R.
authors.forename: A M
authors.initials: AM
authors.lastname: Rubtsov
authors.forename: M B
authors.initials: MB
authors.lastname: Smirnova
authors.forename: A A
authors.initials: AA
authors.lastname: Boldyrev
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D009711:Nucleotides; D002110:Caffeine; D002118:Calcium