title: Genetic characterization of kanamycin resistance in Campylobacter coli.
abstract: The restriction map of plasmid pIP1433 from Campylobacter coli BM2509, isolated in France, was constructed and the location of the kanamycin (Km) and tetracycline (Tc) resistance markers determined using probes for aphA-3 and tetO genes, respectively. These DNA probes were used to study a second Km- and Tc-resistant strain C. coli UA696, isolated in Canada. In this strain, the Km resistance determinant was located in the chromosome. Like pIP1433, the Km resistance determinant in C. coli UA696 specified a 3'-aminoglycoside phosphotransferase of type III whereas the tetO gene was located on a non-transmissible plasmid.
authors.affiliation: Department of Microbiology, University of Alberta, Edmonton, Canada.
authors.forename: D E
authors.initials: DE
authors.lastname: Taylor
authors.forename: W
authors.initials: W
authors.lastname: Yan
authors.forename: L K
authors.initials: LK
authors.lastname: Ng
authors.forename: E K
authors.initials: EK
authors.lastname: Manavathu
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D002167:Campylobacter / Q000235:genetics*; D002874:Chromosome Mapping; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D005819:Genetic Markers; D007613:Kanamycin Resistance / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013753:Tetracycline Resistance / Q000235:genetics
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D004262:DNA Restriction Enzymes