Partial purification of a sodium pump inhibitor from bovine adenohypophysis. Its comparison with the natriuretic factor isolated from hypothalamus.
Adenohypophysis and hypothalamic bovine tissues were homogenized, lipid extracted, salt removed and loaded onto 2 successive mu Bondapak HPLC columns, semipreparative and analytic, respectively. In vitro sodium-pump inhibitory activity, recovered from each tissue, showed similar chromatographic patterns, but hypothalamus seems to contain a major hydrophobic material which appears at the end of the run, when acetonitrile gradient raised 40% approximately. Digitalis-like activity disappears along the purification procedure, and this fact suggests a clear dissociation between (Na/K)ATPase inhibition and digoxin-like activity, measured as crossing with digoxin antibodies.
Illescas
M
M
Dept. of Endocrinology, Ramón y Cajal Hospital, Madrid, Spain.
Ricote
M
M
Méndez
E
E
Robles
R G
RG
Sancho
J M
JM
D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation; D007031:Hypothalamus / Q000032:analysis*; D045283:Natriuretic Agents / Q000302:isolation & purification* / Q000502:physiology; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D045283:Natriuretic Agents; D000254:Sodium-Potassium-Exchanging ATPase