title: Resonance Raman spectroscopy indicates a lysine as the sixth iron ligand in cytochrome f.
abstract: The resonance Raman spectrum of turnip cytochrome f is similar to that of other c-type cytochromes with the exception of a single band at 1532 cm-1 which is shifted to lower frequency relative to its position (1542-1545 cm-1) in other c-type cytochromes. Comparison of the frequency of this band with that in alkylated cytochrome c at high pH suggests that the sixth heme iron ligand in cytochrome f is a deprotonated lysine amino group rather than a methionine sulfur. Comparison of the amino-acid sequences of cytochromes f and c1 suggests lysine-145 as a likely candidate for the sixth heme iron ligand in cytochrome f.
authors.affiliation: University of Arkansas, Fayetteville, AR 72701.
authors.forename: D J
authors.initials: DJ
authors.lastname: Davis
authors.forename: M K
authors.initials: MK
authors.lastname: Frame
authors.forename: D A
authors.initials: DA
authors.lastname: Johnson
mesh_terms: D000595:Amino Acid Sequence; D003580:Cytochromes / Q000032:analysis*; D003575:Cytochromes c1; D045348:Cytochromes f; D008239:Lysine / Q000032:analysis*; D008969:Molecular Sequence Data; D010944:Plants; D013059:Spectrum Analysis, Raman
chemical_list: D003580:Cytochromes; D003575:Cytochromes c1; D045348:Cytochromes f; D008239:Lysine