Age-related changes in the activities of the amine metabolizing enzymes of rat eye.
Eye MAO-A, MAO-B, semicarbazide-sensitive amine oxidase (SSAO) and aldehyde reductase (AR) activities were measured in young and old rats. When enzyme activity is expressed as nmol (mg protein)-1 min-1, a significant decrease (18-23%) of SSAO activity in the eye of old rats was found, whereas there was no significant difference in MAO-A and MAO-B activities. A significant increase of AR activity with D-xylose (67%), DL-glyceraldehyde (64%), D-glucuronate (43%) and D-glucose (21%) was found in the eye of old rats. These results suggest that changes in the activities of the amine metabolizing enzymes of rat eye with age might have consequences for their function in senescence; particularly, the increase of AR activity might be involved in cataract formation.
Cao Danh
H
H
Strolin Benedetti
M
M
Mousset
A
A
Pasquier-Béchet
P
P
D000375:Aging; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing); D000588:Amines / Q000378:metabolism*; D000818:Animals; D001595:Benzylamine Oxidase / Q000378:metabolism; D003010:Clorgyline / Q000494:pharmacology; D003641:Deamination; D005123:Eye / Q000201:enzymology*; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012663:Semicarbazides / Q000494:pharmacology
D000588:Amines; D012663:Semicarbazides; D000429:Alcohol Oxidoreductases; C019642:alcohol dehydrogenase (NADP+); D001595:Benzylamine Oxidase; D006631:Amine Oxidase (Copper-Containing); D008995:Monoamine Oxidase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D003010:Clorgyline