[Establishment and characterization of a human cholangiocellular carcinoma cell line].
A cell line, HuH-28 was established in vitro from a patient with cholangiocellular carcinoma (CCC). This cell line has been in continuous culture over 10 month period with slow growth potential. HuH-28 was composed of spindle-shaped cells as major population besides a small percentage of polygonal-shaped cells. Chromosome number of the cells were distributed near the hypotriploid region on the 3rd passage. HuH-28 cells were not transplantable into nude mice, but secreted some tumor markers including alkaline phosphatase (ALP), gamma glutamyltranspeptidase (GGT), beta 2-microglobulin (BMG), ferritin, elastase-1 and tissue polypeptide antigen (TPA). This HuH-28 cell line will represent a good model for the investigation of carcinogenesis, histogenesis+ and diagnosis of CCC.
Kusaka
Y
Y
Division of Pathology, Cancer Institute, Medical School, Okayama University.
Muraoka
A
A
Tokiwa
T
T
Sato
J
J
D002759:Adenoma, Bile Duct / Q000473:pathology*; D000328:Adult; D000818:Animals; D001650:Bile Duct Neoplasms / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis; D005260:Female; D006801:Humans; D051379:Mice; D051381:Rats; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor