Transfer of IncP plasmids into Stigmatella aurantiaca leading to insertional mutants affected in spore development.
Derivatives of the broad-host-range plasmid RP4, containing the wild-type or modified transposon Tn5 were transferred by conjugation to various Stigmatella aurantiaca isolates. The transposons and in some cases fragments of the plasmid as well were integrated into the chromosome. Thus, insertional mutants have been obtained affected in spore formation in liquid culture.
Glomp
I
I
Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany.
Saulnier
P
P
Guespin-Michel
J
J
Schairer
H U
HU
D015139:Blotting, Southern; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D009154:Mutation; D009231:Myxococcales / Q000235:genetics* / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D013171:Spores, Bacterial / Q000502:physiology
D004251:DNA Transposable Elements; D004269:DNA, Bacterial