title: Effect of single amino acid substitutions on the protease susceptibility of tryptophan synthase alpha subunit.
abstract: In order to explore the correlation between protease susceptibility and conformational stability of a protein, the proteolytic degradation by trypsin, subtilisin and pronase P of the wild-type alpha subunit of tryptophan synthase from Escherichia coli and of its two mutant proteins was studied by measuring circular dichroism at 222 nm at various pH values at 37 degrees C. The mutant proteins are substituted by Gln or Met in place of Glu at position 49. The single amino acid substitutions at position 49 significantly affected susceptibility of this protein to the three proteases. Dependence of protease susceptibility of the wild-type and the two mutant proteins on pH was characteristic of each protein and similar for the three proteases. Comparison of the present results with the conformational stabilities of the three proteins previously measured shows that the order of resistance to the proteases among the three proteins coincides with the order of the values of unfolding Gibbs energy change, suggesting that protein degradation depends upon the conformational stability of a protein.
authors.forename: K
authors.initials: K
authors.lastname: Ogasahara
authors.forename: S
authors.initials: S
authors.lastname: Tsunasawa
authors.forename: Y
authors.initials: Y
authors.lastname: Soda
authors.forename: K
authors.initials: K
authors.lastname: Yutani
authors.forename: Y
authors.initials: Y
authors.lastname: Sugino
mesh_terms: D005971:Glutamates; D018698:Glutamic Acid; D005973:Glutamine; D046911:Macromolecular Substances; D008715:Methionine; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism*; D011402:Pronase / Q000378:metabolism; D011487:Protein Conformation; D013381:Subtilisins / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D014367:Tryptophan Synthase / Q000378:metabolism*
chemical_list: D005971:Glutamates; D046911:Macromolecular Substances; D005973:Glutamine; D018698:Glutamic Acid; D008715:Methionine; D010447:Peptide Hydrolases; D013381:Subtilisins; D014357:Trypsin; D011402:Pronase; D014367:Tryptophan Synthase