Biochemical characterization of domain-specific glycoproteins of the rat hepatocyte plasma membrane.
Seven integral proteins (CE 9, HA 21, HA 116, HA 16, HA 4, HA 201, and HA 301) were isolated from rat hepatocyte plasma membranes by immunoaffinity chromatography on monoclonal antibody-Sepharose. Six of the proteins (all but HA 16) exhibit domain-specific localizations (either bile canalicular or sinusoidal/lateral) about the hepatocyte surface. We identified three of these protein antigens as leucine aminopeptidase (HA 201), dipeptidyl peptidase IV (HA 301), and the asialoglycoprotein receptor (HA 116). We also developed 125I-lectin blotting procedures that, when used in conjunction with chemical and glycosidase treatments, permitted a comparison of the types of oligosaccharides present on the seven proteins. All seven are sialoglycoproteins, based upon the effects of prior neuraminidase and periodate-aniline-cyanoborohydride treatments of blots on labeling by 125I-wheat germ agglutinin. 125I-labeled Ricinus communis agglutinin I and 125I-peanut agglutinin blotting of the desialylated proteins revealed few if any conventional O-linked oligosaccharides, suggesting that the sialyl residues represent termini of N-linked complex-type oligosaccharides. Depending upon the protein, we estimated the presence of 2-26 N-linked oligosaccharides/polypeptide chain from the Mr reductions accompanying chemical or enzymatic deglycosylation. Three of these mature plasma membrane proteins (HA 21, HA 116, and HA 4) have both high mannose-type and complex-type oligosaccharides on every copy of their polypeptide chains. The labeling of these three proteins by 125I-concanavalin A was sensitive to treatment with endoglycosidase H, and each exhibited a quantitative reduction in Mr after the treatment, as assessed independently by 125I-wheat germ agglutinin blotting. At this level of analysis, we were unable to discern differences in the types of oligosaccharides present on these seven glycoproteins that correlate with their patterns of expression within the plasma membrane domains of this polarized epithelial cell.
Bartles
J R
JR
Braiterman
L T
LT
Hubbard
A L
AL
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D037263:Asialoglycoprotein Receptor; D002236:Carbohydrate Conformation; D002462:Cell Membrane / Q000032:analysis*; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D007158:Immunologic Techniques; D007457:Iodine Radioisotopes; D037102:Lectins; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008358:Mannose / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology; D008698:Mesylates / Q000494:pharmacology; D051379:Mice; D009844:Oligosaccharides / Q000032:analysis; D051381:Rats; D011971:Receptors, Immunologic / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D037263:Asialoglycoprotein Receptor; D006023:Glycoproteins; D007457:Iodine Radioisotopes; D037102:Lectins; D008565:Membrane Proteins; D008698:Mesylates; D009844:Oligosaccharides; D011971:Receptors, Immunologic; D012795:Sialoglycoproteins; D003208:Concanavalin A; D007931:Leucyl Aminopeptidase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4; C012077:trifluoromethanesulfonic acid; D008358:Mannose