Effect of a viral rep gene on transformation of cells by an adeno-associated virus vector.
Adeno-associated virus (AAV) vectors readily express the gene for geneticin-resistance under control of the AAV p40 promoter when chromosomally integrated at low copy number in mammalian cells. We show that a truncated AAV rep gene, transcribed from the p5 and p19 promoters, mediates a negative effect on expression of geneticin-resistance in human 293 cells and a positive effect in HeLa cells. Also, we describe a novel phenotype for a mutant expressing the p19 rep gene alone which has a negative effect in 293 cells but no positive effect in HeLa cells.
Mendelson
E
E
Laboratory of Molecular and Cellular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, Bethesda, Maryland 20892.
Smith
M G
MG
Miller
I L
IL
Carter
B J
BJ
D002460:Cell Line; D000229:Dependovirus / Q000235:genetics*; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005822:Genetic Vectors; D006367:HeLa Cells; D012045:Regulatory Sequences, Nucleic Acid; D014170:Transformation, Genetic; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins