[Monoclonal antibodies to cytoplasmic tetrodotoxin-sensitive brain protein. The effect of veratrine on antibody binding to neuroblastoma cells].
Recently a glycoprotein capable to induce tetrodotoxin-sensitive sodium permeability being incorporated to liposomes was purified from the cytoplasm of the bovine brain. It is shown that a monoclonal antibody derived against this protein binds intact murine neuroblastoma cells. Veratrine, neurotoxin referred to modulate the activity of voltage-gated sodium channels, is shown to compete with the antibody for the neuroblastoma surface epitope. It is postulated that molecular moiety bound with the antibody is either identical or spatially related to veratrine (veratridine) binding site.
Pinchuk
G V
GV
Pinchuk
L N
LN
Gerasimenko
O V
OV
Klering
P G
PG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity / Q000187:drug effects; D001666:Binding Sites, Antibody / Q000187:drug effects*; D001667:Binding, Competitive / Q000187:drug effects; D001921:Brain / Q000276:immunology* / Q000378:metabolism; D002417:Cattle; D003593:Cytoplasm / Q000276:immunology*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D012604:Scorpion Venoms / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014702:Veratrine / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012604:Scorpion Venoms; D015222:Sodium Channels; D013779:Tetrodotoxin; D014702:Veratrine
Monoklonal'nye antitela k tsitoplazmaticheskomu tetrodotoksinchuvstvitel'nomu belku golovnogo mozga. Vliianie veratrina na sviazyvanie antitel s kletkmai neĭroblastomy.