The effect of ouabain, insulin, and cyclic AMP on the acidification of luminal fluid in the proximal tubule of bullfrog kidney.
A transient urinary acidification was induced in the bullfrog proximal tubule by the peritubular administration of ouabain (10(-4) M). Insulin (200 mIU/ml) provoked a prolonged decrease of tubular fluid pH (TFpH), whereas dibutyryl cyclic AMP (10(-4) M) produced a transient increase of TFpH. The above data support the view that the urinary acidification in the proximal tubule is not explained by a simple mechanism, such as Na+/H+ exchange.
Kubota
T
T
Department of Physiology, Osaka Medical College, Japan.
Hagiwara
N
N
Inoue
A
A
Fujimoto
M
M
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D000242:Cyclic AMP / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010042:Ouabain / Q000494:pharmacology*; D011892:Rana catesbeiana; D012016:Reference Values
D007328:Insulin; D010042:Ouabain; D003994:Bucladesine; D000584:Amiloride; D000242:Cyclic AMP