Stability and storage characteristics of enzymes in sheep blood.
The stability and storage characteristics were studied of 11 ovine enzymes of potential clinical significance, namely, aldolase, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, acetylcholinesterase, creatine kinase, gamma glutamyltransferase, glutathione peroxidase (GSH-Px), alpha-hydroxybutyrate dehydrogenase, lactate dehydrogenase and superoxide dismutase (SOD). Enzyme activities in fresh serum were compared with those in plasma containing various anticoagulants including lithium heparin, EDTA and oxalate/fluoride. The same preservatives were assessed for their effects on the whole blood activities of GSH-Px and SOD. Stabilities of enzymes in plasma and serum stored at room (+20 degrees C), refrigerator (4 degrees C) or deep freeze (-20 degrees C) temperatures were also compared. In addition, SOD and GSH-Px activities in samples stored, at the same temperatures, as whole blood or aqueous lysates were monitored. The results are discussed with particular reference to the differences between sheep and cattle.
Jones
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D000110:Acetylcholinesterase / Q000097:blood; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D001800:Blood Specimen Collection / Q000662:veterinary; D003402:Creatine Kinase / Q000097:blood; D004355:Drug Stability; D004798:Enzymes / Q000097:blood*; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006884:Hydroxybutyrate Dehydrogenase / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D011309:Preservation, Biological; D012756:Sheep / Q000097:blood*; D013482:Superoxide Dismutase / Q000097:blood; D013696:Temperature; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000097:blood
D000925:Anticoagulants; D004798:Enzymes; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase; D005634:Fructose-Bisphosphate Aldolase