[Plasmids containing 2 new variants of the E.coli lacZ gene].
New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess beta-galactosidase activity. Use of these plasmids as new expression vectors is suggested.
Khudiakov
Iu E
IuE
Kalinina
T I
TI
Nepliueva
V S
VS
Smirnov
V D
VD
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D008969:Molecular Sequence Data; D010957:Plasmids
D004262:DNA Restriction Enzymes
Plazmidy, soderzhashchie dva novykh varianta gena lacZ E. coli.