title: Role of stearoyl-CoA desaturase and 1-acylglycerophosphorylcholine acyltransferase in the regulation of the acyl composition of phosphatidylcholine in rat liver.
abstract: The role of stearoyl-CoA desaturase and 1-acylglycerophosphorylcholine (1-acylGPC) acyltransferase in regulating acyl composition of microsomal phosphatidylcholine was investigated in rat liver, using rats in five different kinds of physiological state: clofibric acid-fed rats, diabetic rats, insulin-treated diabetic rats, starved rats and starved-refed rats. There was a reverse linear correlation between 18:1 and 18:2 in the C-2 position, and a similar correlation was found between 18:1 and 18:2 in microsomal free fatty acids. The proportion of 18:1 or 18:2 in the C-2 position of phosphatidylcholine correlated with the proportion of the respective fatty acids in microsomal free unsaturated fatty acids which could be incorporated effectively, except for the group of clofibric acid-fed rats. In this group alone, 1-acylGPC acyltransferase was induced markedly. The proportion of 18:1 in microsomal free fatty acids correlated well with the activity of stearoyl-CoA desaturase. The physiological significance of stearoyl-CoA desaturase and 1-acylGPC acyltransferase was discussed in relation to the regulation of the acyl composition of phosphatidylcholine.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawashima
authors.forename: A
authors.initials: A
authors.lastname: Hirose
authors.forename: T
authors.initials: T
authors.lastname: Adachi
authors.forename: H
authors.initials: H
authors.lastname: Kozuka
mesh_terms: D008243:1-Acylglycerophosphocholine O-Acyltransferase; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002995:Clofibric Acid / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005502:Food; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000201:enzymology; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*
chemical_list: D002995:Clofibric Acid; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase; C031076:2-acylglycerophosphocholine acyltransferase