Expression of type 1 pili by Escherichia coli strains of high and low virulence in the intestinal tract of gnotobiotic turkeys.
Highly virulent (strain 1) and weakly virulent (strain 3) Escherichia coli were examined using immunofluorescent and electron microscopic techniques to determine their ability to express type 1 pili in the intestinal tract of 3-week-old gnotobiotic turkeys. Turkeys were necropsied on postinoculation day (PID) 1, 2, 5, 8, and 12. Nonpiliated forms of strains 1 and 3 were more numerous than piliated forms in cecal and colonic contents examined by negative staining electron microscopy. A piliated form of strain 1 was seen in intestinal contents on each PID and was more numerous in cecal contents than in colonic contents. The mucus blanket of the cecum and colon contained large numbers of bacteria, although organisms were rarely intimately associated with the intestinal epithelium. Immunofluorescent staining indicated large numbers of piliated forms of strains 1 and 3 within the mucus blanket of the cecum and colon on PID 2, 5, 8, and 12. Piliated bacteria were infrequently seen in the ileal mucus blanket. Serum antibody titers to type 1 pili increased markedly by PID 5 and persisted in turkeys inoculated with strain 1. In contrast, antibody titers in turkeys exposed to strain 3 increased gradually and varied markedly among birds at each PID. Type 1 pili may not be important for adherence of pathogenic E coli to intestinal epithelium of turkeys.
Dominick
M A
MA
Schmerr
M J
MJ
Jensen
A E
AE
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000276:immunology / Q000472:pathogenicity* / Q000648:ultrastructure; D010861:Fimbriae, Bacterial / Q000276:immunology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005856:Germ-Free Life; D006386:Hemagglutination Tests / Q000662:veterinary; D007413:Intestinal Mucosa / Q000382:microbiology; D007422:Intestines / Q000382:microbiology*; D008854:Microscopy, Electron; D014422:Turkeys / Q000276:immunology / Q000382:microbiology*; D014774:Virulence
D000907:Antibodies, Bacterial