Leukotriene A4, conversion to leukotriene B4 in human T-cell lines.
Human T-cell lines (HSB, MOLT-4 and CCRF-CEM) produced leukotriene B4 when incubated with leukotriene A4. The product was characterized by chromatographic properties, UV-spectroscopy and gas chromatography mass spectrometry. About 10 pmol of leukotriene B4 was obtained per 10(6) cells. When incubated with arachidonic acid plus the calcium ionophore A23187 however, no leukotriene B4 was found, indicating that the T-cell lines lack 5-lipoxygenase yet contain LTA4 hydrolase.
Fu
J Y
JY
Dept. of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
Medina
J F
JF
Funk
C D
CD
Wetterholm
A
A
Rådmark
O
O
D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D017572:Leukotriene A4; D007975:Leukotriene B4 / Q000378:metabolism*; D015289:Leukotrienes / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D017572:Leukotriene A4; D015289:Leukotrienes; D007975:Leukotriene B4; D000001:Calcimycin