title: Down-regulation of interferon-gamma-induced class II expression on human glioma cells by recombinant interferon-beta: effects of dosage treatment schedule.
abstract: We have examined the influence of human recombinant interferon-beta (IFN-beta) and interferon-gamma (IFN-gamma) on class II antigen expression on cultured glioblastoma multiforme cells by flow cytometry. Class II molecules were not constitutively expressed on these cells, nor induced by IFN-beta. IFN-gamma increased class II expression in a dose-dependent fashion. We demonstrate that IFN-beta is either antagonistic or synergistic with IFN-gamma in class II induction depending upon dose and schedule of administration. Both interferons at 100 IU/ml reduce class II expression by 18%, compared to IFN-gamma alone. Pretreatment with IFN-beta for 72 h, followed by both interferons yielded a 90% reduction. In contrast, lower concentrations (10 IU/ml) of both interferons were synergistic.
authors.affiliation: Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107.
authors.forename: J
authors.initials: J
authors.lastname: Joseph
authors.forename: R L
authors.initials: RL
authors.lastname: Knobler
authors.forename: C
authors.initials: C
authors.lastname: D'Imperio
authors.forename: F D
authors.initials: FD
authors.lastname: Lublin
mesh_terms: D004334:Drug Administration Schedule; D004338:Drug Combinations; D004357:Drug Synergism; D005434:Flow Cytometry; D005909:Glioblastoma / Q000276:immunology* / Q000473:pathology; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
chemical_list: D004338:Drug Combinations; D006684:HLA-DR Antigens; D007370:Interferon Type I; D011994:Recombinant Proteins; D007371:Interferon-gamma