title: Detection of animal and human enteric viruses in water from the Assomption River and its tributaries.
abstract: Animal enteroviruses, reoviruses, and human enteric viruses were detected in water samples (20 L) from a major river system, the Assomption River in the province of Quebec. Animal enteroviruses, probably of porcine origin (this region is a major producer of pork), were isolated on porcine cell cultures and were found in 29 to 60% of water samples from the different sites on the river and in 19 to 48% of the water samples from the tributaries. The average concentration of these animal enteroviruses in water from the Assomption River was 2 to 7 mpniu/L (most probable number of infectious units per litre), and that from the tributaries varied from 3 to 24 mpniu/L. Reoviruses were detected in infected cell cultures by an enzyme-linked immunosorbent assay. Their origin is probably avian (broiler chicken farms) or human (untreated domestic waste waters) and they were detected in 19 to 52% of the water samples from the Assomption River at an average concentration of 3 to 12 mpniu/L. In water samples from the tributaries, 5 to 71% of the samples were positive at an average concentration of 5 to 24 mpniu/L. Human enteric viruses were detected in MA-104 cells by an immunoperoxidase assay using human immune serum globulin. They were detected in 13 to 72% of water samples from the Assomption River and 14 to 71% of the water samples from the tributaries. The average concentration of these human enteric viruses in Assomption River water varied from 1 to 12 and from 2 to 145 mpniu/L in water samples from the tributaries.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institut Armand-Frappier, Université du Québec, Ville de Laval, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Payment
authors.forename: F
authors.initials: F
authors.lastname: Affoyon
authors.forename: M
authors.initials: M
authors.lastname: Trudel
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D004770:Enterovirus / Q000302:isolation & purification*; D005618:Fresh Water; D006801:Humans; D011792:Quebec; D012087:Reoviridae / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification*; D014871:Water Microbiology