Production of active human carbonic anhydrase II in E. coli.
cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.
Forsman
C
C
Department of Biochemistry, University of Umeå, Sweden.
Behravan
G
G
Osterman
A
A
Jonsson
B H
BH
D000595:Amino Acid Sequence; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism
D011994:Recombinant Proteins; D004247:DNA; D004262:DNA Restriction Enzymes; D002256:Carbonic Anhydrases