ACTH stimulates fructose 2,6-bisphosphate synthesis and glycolysis in Y-1 adrenal tumor cells.
The effect of ACTH on glycolysis has been studied in Y-1 tumor adrenal cells. ACTH caused a sustained increase in the liberation of lactate as well as a stimulation of both basal and glucose-induced fructose 2,6-bisphosphate content. ACTH produces changes also in the activities of phosphofructokinase-1 and phosphofructokinase-2. The addition of Ca2+ or dibutyryl cyclic AMP did not modify neither lactate production nor fructose 2,6-bisphosphate levels. The results suggest that fructose 2,6-bisphosphate regulates ACTH-induced glycolysis at the phosphofructokinase-1 step, although the biochemical mechanism of phosphofructokinase-2 activation remains elusive.
de Miguel
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C
Department of Biochemistry, Faculty of Medicine, University of Sevilla, Spain.
Gualberto
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A
Sobrino
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F
D000310:Adrenal Gland Neoplasms / Q000201:enzymology / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D005635:Fructosediphosphates / Q000096:biosynthesis* / Q000502:physiology; D005947:Glucose / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects*; D006598:Hexosediphosphates / Q000096:biosynthesis*; D007773:Lactates / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000378:metabolism; D014407:Tumor Cells, Cultured
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D007773:Lactates; D003994:Bucladesine; C027652:fructose 2,6-diphosphate; D000324:Adrenocorticotropic Hormone; D010732:Phosphofructokinase-1; D005947:Glucose; D002118:Calcium