Cellular mechanism of atrial natriuretic factor secretion by cultured rat cardiocytes.
Using primary culture of atrial cardiocytes from neonatal rats, we have demonstrated that alpha 1-adrenergic and muscarinic cholinergic agonists have a direct stimulatory effect on secretion of atrial natriuretic factor (ANF) and that ANF secretion is stimulated by phorbol ester, Ca2+ ionophore, high K+-induced depolarization and Ca2+-channel agonists. These data suggest that receptor-mediated mobilization of intracellular Ca2+ and activation of protein kinase C as well as Ca2+ influx via voltage-dependent Ca2+ channels are involved in the secretory mechanism of ANF.
Hirata
Y
Y
Hypertension-Endocrine Division, National Cardiovascular Center Research Institute, Osaka, Japan.
Matsubara
H
H
Fukuda
Y
Y
Yoshimi
H
H
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology; D002478:Cells, Cultured; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects
D015220:Calcium Channels; C472610:atrial natriuretic peptide, rat; D009320:Atrial Natriuretic Factor; D011188:Potassium