title: Mechanism of action of the antiviral compound MDL 20,610.
abstract: The purpose of this study was to probe the antirhinovirus (RV) mechanism of action of MDL 20,610. Evaluation of the compound's effects on RV RNA synthesis, uncoating of neutral red-sensitized RV, plasma membrane penetration by RV, stabilization of RV against heat (56 degrees C) and low pH (5.0) inactivation, and studies with MDL 20,610-resistant RV mutants indicate that MDL 20,610 binds directly to the RV capsid with subsequent inhibition of acid-mediated virion uncoating.
authors.affiliation: Department of Pharmacology, Merrell Dow Research Institute, Indianapolis, Indiana 46268-0470.
authors.forename: M T
authors.initials: MT
authors.lastname: Kenny
authors.forename: H L
authors.initials: HL
authors.lastname: Torney
authors.forename: J K
authors.initials: JK
authors.lastname: Dulworth
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D011725:Pyridines / Q000494:pharmacology*; D012367:RNA, Viral / Q000096:biosynthesis; D012229:Rhinovirus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D011725:Pyridines; D012367:RNA, Viral; C050725:MDL 20610