title: [IgA protease activity of microbes in the genus Bordetella].
abstract: Monoclonal IgA paraproteins of subclasses 1 and 2, isolated from the sera of myeloma patients, were incubated for 4, 24, 48 and 72 hours with B. pertussis, B. parapertussis, B. bronchiseptica cultures, as well as Haemophilus influenzae strain. The fragmentation of IgA was studied by immunielectrophoresis with antisera to alpha-chain, to Fab alpha + Fc alpha, to Fab alpha and with antisera to light chains corresponding to the type of paraprotein. B. pertussis and B. parapertussis were found to have subclass-unspecific IgA protease which splitted off a cathode fragment, similar to Fab-fragment and, probably, corresponding to the variable domain of alpha-chain (Fv), after 48-hour incubation. Similar IgA protease was detected in H. influenzae, found to have classical IgA1 protease as well. All Bordetella species under study splitted off anode components from IgA paraproteins of both subclasses. These components, containing the determinants of heavy and light IgA chains, were either IgA - alpha I-antitrypsin complexes or some IgA fragments with high electrophoretic motility. None of the strains under study splitted monoclonal IgG.
authors.forename: R G
authors.initials: RG
authors.lastname: Shakirova
authors.forename: G P
authors.initials: GP
authors.lastname: German
authors.forename: E V
authors.initials: EV
authors.lastname: Chernokhvostova
mesh_terms: D000911:Antibodies, Monoclonal / Q000502:physiology; D001884:Bordetella / Q000201:enzymology*; D001886:Bordetella pertussis / Q000201:enzymology; D006193:Haemophilus influenzae / Q000201:enzymology; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D009101:Multiple Myeloma / Q000276:immunology; D010447:Peptide Hydrolases / Q000302:isolation & purification* / Q000494:pharmacology; D012697:Serine Endopeptidases; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase
vernacular_title: IgA-proteaznaia aktivnost' mikrobov roda Bordetella.