Comparison of the solid phase enzyme receptor assay (SPERA) and the microbiological assay for teicoplanin.
A solid phase enzyme receptor assay (SPERA) for teicoplanin has been developed and recently presented in a kit form. The assay relies on the competition between antibiotic present in the biological fluid and peroxidase labelled teicoplanin for albumin-epsilon-amino-caproyl-D-alanyl-D-alanine (BSA-epsilon-ACA-D-Ala-D-Ala), a synthetic analog of the biological receptor. The kit contains BSA-epsilon-ACA-D-Ala-D-Ala coated microplates together with all the necessary reagents. The limit of detection of the SPERA is 0.5 mg 1(-1). The microbiological assay for teicoplanin uses Bacillus subtilis ATCC 6633 as test organism and a high salt medium to ensure a low limit of detection of 0.05 mg 1(-1). The previously established correlation between the two methods has been confirmed using the kits on 280 serum and 216 urine samples with concentrations up to 500 mg 1(-1).
Cavenaghi
L
L
Corti
A
A
Cassani
G
G
D000900:Anti-Bacterial Agents / Q000097:blood* / Q000652:urine; D001412:Bacillus subtilis / Q000378:metabolism; D001681:Biological Assay; D006020:Glycopeptides / Q000097:blood / Q000652:urine; D006801:Humans; D008826:Microbial Sensitivity Tests; D017334:Teicoplanin
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D017334:Teicoplanin