A cloned MCGF cDNA encodes a multilineage hematopoietic growth factor: multiple activities of interleukin 3.
We recently isolated from a mouse T cell cDNA library a full-length clone that encodes a mast cell growth factor (1). On the basis of sequence homologies, this cloned factor must be similar if not identical to purified interleukin 3 (IL 3) (2, 3). Here, we report the first biologic characterization of the cloned gene product expressed in COS-7 monkey cells. Our results establish that a single molecular species promotes the growth and differentiation of a wide spectrum of hematopoietic cell types, including multipotential stem cells and various committed progenitor cells. The relationship of cloned IL 3 to other colony-stimulating factors is discussed.
Rennick
D M
DM
Lee
F D
FD
Yokota
T
T
Arai
K I
KI
Cantor
H
H
Nabel
G J
GJ
D015089:20-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001854:Bone Marrow Cells; D002460:Cell Line; D003001:Cloning, Molecular; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000235:genetics / Q000502:physiology*; D004247:DNA / Q000378:metabolism*; D006133:Growth Substances / Q000235:genetics / Q000502:physiology*; D000882:Haplorhini; D007377:Interleukin-3; D007668:Kidney; D008222:Lymphokines / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D018800:Thy-1 Antigens; D014162:Transfection
D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D008222:Lymphokines; D018800:Thy-1 Antigens; D004247:DNA; D015089:20-Hydroxysteroid Dehydrogenases