In vitro motility of isolated adults and segments of Onchocerca volvulus, Brugia pahangi and Acanthocheilonema viteae.
Observations were made on the spontaneous motility in vitro of entire adult worms and segments of Onchocerca volvulus, Brugia pahangi and Acanthocheilonema viteae. Segment motility was recorded in a mechano-transducer apparatus and responses of worm tissues to anthelmintics and other pharmacological agents were compared. Entire adult female O. volvulus showed varied patterns of motor activity, ranging from continuous low level whole body motion to periodic spasmodic contractions interspersed between intervals of quiescence lasting from a few to 30 seconds. The same range characterized movements of 1.5-4 cm segments cut from worms liberated from collagenase digested nodules. However, fresh segments dissected directly from surgical specimens were completely inactive due to the paralyzing effect of xylocaine, used as local anesthetic. This effect wore off in 2-5 hours in vitro, and recovered segments behaved in the same way as those from enzymatically liberated worms. Segments of B. pahangi and A. viteae also showed motor activity patterns which reflected those of whole adults. Segments of O. volvulus behaved reproducibly, whether examined in the field in endemic areas, or after transport of nodules or freed worms to Michigan from Guatemala or Sudan. Segments of all worms were unresponsive to most anthelmintics, but O. volvulus was susceptible to paralysis by CGP 6140, levamisole, pyrantel and carbachol at concentrations of ten to a thousand times lower than those required to produce any influence on B. pahangi. Segments of A. viteae more closely resembled O. volvulus in their dose responses to these drugs, although CGP 6140 was without effect.(ABSTRACT TRUNCATED AT 250 WORDS)
Pax
R A
RA
Department of Zoology, Michigan State University, East Lansing.
Williams
J F
JF
Guderian
R H
RH
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology; D002009:Brugia / Q000187:drug effects / Q000502:physiology*; D004153:Dipetalonema / Q000187:drug effects / Q000502:physiology*; D005260:Female; D008297:Male; D003012:Microbial Collagenase; D009068:Movement; D009854:Onchocerca / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D010507:Periodicity
D000871:Anthelmintics; D003012:Microbial Collagenase