Map location on Agrobacterium root-inducing plasmids of homologies with the virulence region of tumor-inducing plasmids.
Southern-type hybridizations were carried out in order to identify sequence homologies with the pTi vir loci, on an agropine-type plasmid (pRiHRI) and a mannopine-type plasmid (pRi8196) of Agrobacterium rhizogenes. The localization of the sequences hybridizing with subcloned fragments containing vir A, B, G, C, and D from pTiAch5 indicated a similar linear organization of the pTi vir loci and their homologies on pRiHRI and pRi8196, though no homology was detected on both pRi with a 1.1-kb internal fragment of virD. No homology was detected either with the vir E locus on pRiHRI vir region, nor with the virF locus on both pRi vir regions. As on nopaline pTiC58, fragments bearing the homologies with virC and virG are closer together on both pRi than on octopine pTiAch5. A preliminary functional map of the pRiHRI vir region is deduced from this study.
Birot
A M
AM
Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Versailles, France.
Casse-Delbart
F
F
D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D010941:Plant Tumors; D010944:Plants / Q000382:microbiology; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics* / Q000472:pathogenicity; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014774:Virulence
D004262:DNA Restriction Enzymes