Inhibition and inactivation of pyruvate phosphate dikinase with Cr(III) complexes of adenosine 5'-triphosphate and inorganic pyrophosphate.
The exchange inert complexes beta,gamma-bidentate Cr(H2O)4ATP and P1,P2-bidentate Cr(H2O)4PP were found to bind to the Bacteriodes symbiosus pyruvate phosphate dikinase ATP and PP binding sites, respectively. The inactivation of the enzyme that was observed with these complexes was shown to involve covalent attachment of the entire complex to the enzyme via insertion of enzyme amino acid side chains into the coordination sphere of the Cr(III). Incubation of Cr(H2O)4ATP with other proteins also resulted in covalent attachment.
Ciskanik
L
L
Department of Chemistry and Biochemistry, University of Maryland, College Park, 20742.
Dunaway-Mariano
D
D
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D001439:Bacteroides / Q000201:enzymology; D007700:Kinetics; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D011772:Pyruvate, Orthophosphate Dikinase / Q000037:antagonists & inhibitors*
D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D011772:Pyruvate, Orthophosphate Dikinase