title: The effect of anticoagulants on Blennius pholis L. Leucocytes.
abstract: The effects of ethylenediamine-tetraacetic acid (EDTA), heparin and tri-sodium citrate (TSC) on various haematological parameters in the blenny, Blennius pholis, were investigated. EDTA and heparin, at the concentrations tested, proved adequate in preventing coagulation although leucocyte viability was reduced when the higher levels were used. TSC failed to prevent coagulation at all concentrations tested. Short (5 min) and long (20 min) term contact of these anticoagulants with blood did not produce any significant changes in leucocyte proportions. Heparin is regarded as the most suitable anticoagulant for use with B. pholis blood.
authors.forename: G
authors.initials: G
authors.lastname: Mainwaring
authors.forename: A F
authors.initials: AF
authors.lastname: Rowley
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D005399:Fishes / Q000097:blood*; D006493:Heparin / Q000494:pharmacology; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects*; D008297:Male
chemical_list: D000925:Anticoagulants; D006493:Heparin; D004492:Edetic Acid