title: Changes in lipid fluidity and fatty acid composition with altered culture temperature in Tetrahymena pyriformis-NT1.
abstract: Cultures of T. pyriformis-NT1 were grown at 20 degrees C (Tg 20 degrees C) and 38 degrees C (Tg 38 degrees C). G.L.C. analysis and D.P.H. fluorescence polarization measurements in extracted phospholipids indicated that there was increased saturation of fatty acids and relatively reduced fluidity as growth temperature was increased. Breakpoints occurred in the Arrhenius plots of fluorescence polarization at 16 degrees C for Tg 38 degrees C total extracted phospholipids and 9 degrees C for Tg 20 degrees C lipids.
authors.forename: J G
authors.initials: JG
authors.lastname: Connolly
authors.forename: I D
authors.initials: ID
authors.lastname: Brown
authors.forename: A G
authors.initials: AG
authors.lastname: Lee
authors.forename: G A
authors.initials: GA
authors.lastname: Kerkut
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism*; D010743:Phospholipids / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D013769:Tetrahymena pyriformis / Q000502:physiology*; D013816:Thermodynamics
chemical_list: D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids