The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli.
The nucleotide sequence of the gsh I gene for gamma-glutamylcysteine synthetase(GSH I) of Escherichia coli B has been determined. The gsh I structural gene contains 1557 bases in length and predicted a ploypeptide of 518 amino acids with a calculated molecular weight of 58,251. The value is in good agreement with that obtained from gel filtration and SDS/PAGE of GSH I. The initiation codon 5 bp downstream of putative Shine-Dalgarno sequence was an unusual TTG, which encoded methionine. For transcription, two sets of consensus promoter signals(-10 and -35 regions) overlapping each other were identified. The terminator signal shows the favored stem-loop structure with an adequate free energy delta G = -22.80 kcal/mol.
Watanabe
K
K
Yamano
Y
Y
Murata
K
K
Kimura
A
A
D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003062:Codon; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005721:Glutamate-Cysteine Ligase / Q000032:analysis*; D005978:Glutathione / Q000096:biosynthesis; D008970:Molecular Weight; D010453:Peptide Synthases / Q000032:analysis*; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D003062:Codon; D004262:DNA Restriction Enzymes; D010453:Peptide Synthases; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione