Evidence in liver for a disulphide-linked scavenger receptor containing a binding site for acetylated low-density lipoprotein and maleylated bovine serum albumin.
Membranes from rat liver were analysed under reducing conditions. The components of the soluble membranes responsible for the binding of acetylated low density lipoprotein (acetyl-LDL) and maleylated bovine serum albumin (Mal-BSA) were chromatographed on a polyethyleneimine-cellulose column and subsequently separated by gel electrophoresis. For both ligands a major binding protein (Mr = 35,000) was revealed by ligand blotting. A minor protein (Mr greater than 67,000) exhibited little binding. The Scatchard plot of the 131I-Mal-BSA binding data of the 35 kDa protein was linear, with a Kd of 17.3 nM. High concentrations of acetyl-LDL competed for half of the 131I-Mal-BSA binding. Excessive Mal-BSA competed for all the visible acetyl-LDL binding. The findings indicate the existence, in the reduced hepatic membrane, of a 35 kDa protein that has two binding sites for 131I-Mal-BSA and one binding site for acetyl-LDL.
Ottnad
E
E
Department of Medicine, University of Heidelberg, Federal Republic of Germany.
Via
D P
DP
Sinn
H
H
Friedrich
E
E
Ziegler
R
R
Dresel
H A
HA
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D015815:Cell Adhesion Molecules; D004220:Disulfides; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008024:Ligands; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D051381:Rats; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011973:Receptors, LDL / Q000378:metabolism*; D051116:Receptors, Scavenger; D012710:Serum Albumin, Bovine / Q000378:metabolism*
D000418:Albumins; D015815:Cell Adhesion Molecules; D004220:Disulfides; D008024:Ligands; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D011973:Receptors, LDL; D051116:Receptors, Scavenger; C032629:acetyl-LDL; C049877:maleylalbumin; C049878:maleylalbumin receptor; D012710:Serum Albumin, Bovine