Cloning and characterization of a cDNA encoding bovine endonexin (chromobindin 4).
Endonexin is a 32kDa, calcium-dependent membrane-binding protein that is one of a group of proteins that binds to chromaffin granule membranes and may regulate membrane fusion events occurring during exocytosis. In this study an oligonucleotide probe that codes for a highly conserved, repeated sequence present in this and related proteins was used to isolate a 2,048 nucleotide cDNA encoding endonexin from a bovine liver cDNA library. The translated amino acid sequence of endonexin shows the four domain structure characteristic of proteins in this class. The nucleotide sequence is 55 to 61% identical to that of the related membrane-binding proteins lipocortin, calpactin, endonexin II and (half of) 68kDa calelectrin. Southern blot analysis of bovine genomic DNA suggests the presence of a single gene for this protein. A consensus nucleotide sequence (TCTGGGAACTTC) was identified in the 5' nontranslated portion of the endonexin mRNA that is also represented in the messages for calpactin and endonexin II.
Hamman
H C
HC
Department of Pharmacology, University of Virginia, Charlottesville 22908.
Gaffey
L C
LC
Lynch
K R
KR
Creutz
C E
CE
D000595:Amino Acid Sequence; D000818:Animals; D017304:Annexin A5; D017302:Annexins; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D008099:Liver / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics
D017304:Annexin A5; D017302:Annexins; D002135:Calcium-Binding Proteins; D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes