New complement fixation test with peroxidase-labeled complement Clq for direct and quantitative determination of antibodies to herpes simplex virus.
An enzyme-labeled complement fixation (ELISA-CF) test for the direct and quantitative determination of complement fixing (CF) antibodies has been developed. This paper described the introduction of the ELISA-CF test that used peroxidase-labeled Clq component of complement to detect CF antibodies which had reacted with herpes simplex virus (HSV), as a virus model. Equal volumes of heat-inactivated serum and the peroxidase-labeled Clq (P*-Clq) were simultaneously added to wells of microplates which had been coated with HSV CF antigen or with cell control antigen. The enzymatic activities of P*-Clq bound to the immune complex were determined photometrically. The ELISA-CF test allows processing of serum specimens in a 3-hr operation, with procedural simplicity and increased specificity and sensitivity compared with the conventional CF test.
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Department of Microbiology, School of Hygienic Sciences, Kitasato University, Kanagawa.
D000914:Antibodies, Viral / Q000032:analysis*; D003166:Complement Activating Enzymes; D003172:Complement C1; D015922:Complement C1q; D003168:Complement Fixation Tests / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010544:Peroxidases; D018139:Simplexvirus / Q000276:immunology*
D000914:Antibodies, Viral; D003172:Complement C1; D015922:Complement C1q; D010544:Peroxidases; D003166:Complement Activating Enzymes