Mdg-1 mobile element polymorphism in selected Drosophila melanogaster populations.
The changes in mdg-1 mobile element polymorphism that followed artificial selection for either high or low egg-to-adult viability in a Drosophila melanogaster population were investigated. The two selected subpopulations were thus characterized for fecundity, wing length, and number and location of the mdg-1 mobile element by in situ hybridization of the biotinylated--DNA on salivary gland chromosomes. The selected populations that differed greatly in egg-to-adult viability showed the same mean fecundity and identical values for intra and inter components of variances, intraclass correlation coefficient, and fluctuating asymmetry estimated on the wing length measurement. This indicates a non-correlated effect between deleterious mutations affecting viability and other fitness components. However, the two selected populations differed in their pattern of mdg-1 location, although the mean number of insertions per genome was not different from that of the initial population; hence, the number of insertions of the mdg-1 mobile element was independent of the effective population size. These results suggest that the mdg-1 copy number was regulated, and that during the selection process, drift and inbreeding made up new insertion patterns of the mdg-1 element in the selected populations. The results are discussed in the light of some recent theoretical models of the population dynamics of transposable elements.
Biémont
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Population biology laboratory, University Lyon, Villeurbanne, France.
Terzian
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D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005298:Fertility; D005808:Genes, Recessive; D005828:Genetics, Population; D007178:Inbreeding; D008297:Male; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D011897:Random Allocation; D012641:Selection, Genetic; D014921:Wings, Animal / Q000033:anatomy & histology
D004251:DNA Transposable Elements