title: Amiloride potentiates the vascular effects of atrial natriuretic factor.
abstract: We have demonstrated an interaction between the effects of amiloride and atrial natriuretic factor (ANF) on the vascular system. In precontracted rabbit aortic strips the relaxant effect of a combination of ANF (10(-10) mol/l) and amiloride (10(-5) mol/l) was synergistic. The production of cyclic (c)GMP, which parallels ANF-induced relaxation of the strips, was not affected by amiloride alone up to 10(-3) mol/l, but was concentration-dependently increased in the presence of 10(-8) mol/l ANF. In spontaneously hypertensive rats (SHR) ANF-induced decreases in blood pressure were potentiated by amiloride. Binding experiments revealed an interaction between amiloride and ANF at the receptor level; binding of labelled ANF to aortic tissue was increased by amiloride but decreased by ATP. These data show that amiloride and ATP influence a mechanism that determines the sensitivity of vessels to ANF and this interaction occurs both at receptor level and at the level of transduction.
authors.affiliation: Hoechst AG, Frankfurt, Federal Republic of Germany.
authors.forename: W
authors.initials: W
authors.lastname: Linz
authors.forename: U
authors.initials: U
authors.lastname: Albus
authors.forename: G
authors.initials: G
authors.lastname: Wiemer
authors.forename: G
authors.initials: G
authors.lastname: Breipohl
authors.forename: J
authors.initials: J
authors.lastname: Knolle
authors.forename: B A
authors.initials: BA
authors.lastname: Schölkens
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000187:drug effects*; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D006152:Cyclic GMP / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D011188:Potassium / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D000584:Amiloride; D009320:Atrial Natriuretic Factor; D000255:Adenosine Triphosphate; D006152:Cyclic GMP; D011188:Potassium