Expression of oncogenes in human hepatoma cell lines.
The expression of 20 known cellular proto-oncogenes in human well-differentiated hepatoma cell line Hep3B and poorly-differentiated hepatoma cell line HA22T/VGH was studied by Northern blot hybridization. Among the cellular proto-oncogenes examined, both cell lines express protein kinase genes including fps, mos and raf; PDGF B chain sis gene; GTP/GDP binding protein gene Ha-ras and nuclear protein genes including fos and myc. The expression of yes, abl, ros, src, erb-B, erb-A, fms, Ki-ras, myb, rel and bas genes was not detected in both cell lines.
Ting
L P
LP
Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei, Taiwan, ROC.
Jeng
K S
KS
Chou
C K
CK
Su
T S
TS
Hu
C P
CP
Wong
F H
FH
Chang
H K
HK
Chang
C M
CM
D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation; D002460:Cell Line; D015342:DNA Probes; D019204:GTP-Binding Proteins / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020574:Proto-Oncogene Proteins c-sis; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000032:analysis
D015342:DNA Probes; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D012334:RNA, Neoplasm; D011494:Protein Kinases; D019204:GTP-Binding Proteins