The effect of Ca++ and Mg++ on the action of Clostridium perfringens enterotoxin on Vero cells.
Clostridium perfringens enterotoxin binds to receptors on Vero cells. This process does not depend on the presence of divalent cations (Ca++, Mg++). Binding of enterotoxin causes inhibition of 14C-leucine incorporation into proteins, probably because of depression of amino acid transport. The presence of Mg++ speeds up this effect of the enterotoxin. The enterotoxin produces membrane leakage only in the presence of Ca++, but additional Mg++ increases the rate of this process. These results indicate that the dissociation constant of the enterotoxin receptor interaction is reduced in the presence of Mg++. A model for the mode of action of the enterotoxin is proposed.
Granum
P E
PE
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology*; D002413:Cations, Divalent; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D003016:Clostridium perfringens / Q000472:pathogenicity*; D004768:Enterotoxins / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology*; D014176:Protein Biosynthesis
D000596:Amino Acids; D002413:Cations, Divalent; D004768:Enterotoxins; D007770:L-Lactate Dehydrogenase; D008274:Magnesium; D002118:Calcium