The analysis of low levels of gamma-glutamyltransferase activity by high-performance liquid chromatography with electrochemical detection.
A method for the analysis of low levels of the enzyme gamma-glutamyltransferase in biological samples by high-performance liquid chromatography with electrochemical detection is described. A gamma-glutamyl moiety from glutathione is transferred by the enzyme to glycylglycine to produce a tripeptide which is assayed directly after a purification step using octadecylsilica. Confirmation of the method is by use of the inhibitor AT-125. The method is used to measure the level of enzyme activity in rodent tissues and in cultured cells.
Hoskins
J A
JA
Davies
F F
FF
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D008099:Liver / Q000201:enzymology; D051379:Mice; D009842:Oligopeptides; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D009842:Oligopeptides; C048439:gamma-glutamyl-glycyl-glycine; D005723:gamma-Glutamyltransferase