Studies on satellite nucleoli in human normal and leukemic lymphocytes.
Lymphocytes from normal and leukemic patients, and phytohemagglutinin-stimulated lymphocytes were investigated by means of immunofluorescence procedures and a silver reaction for the demonstration of proteins characteristic of nucleolus organizer regions in interphasic cells to provide basic information on the presence of satellite nucleoli in these cells. The results clearly indicated that satellite nucleoli are present in a limited but constant percentage of peripheral lymphocytes. An increased percentage of lymphocytes with satellite nucleoli was found only in leukemic patients and after silver staining. In contrast, a decreased percentage of satellite nucleoli was found 24 h after stimulation with phytohemagglutinin vitro. In leukemic patients, the discrepancy in the percentage of lymphocytes with satellite nucleoli between immunostained and silver-stained preparations may suggest that the silver reaction demonstrates the presence of an additional argyrophilic protein besides proteins B23 and C23, or altered forms of these proteins, which does not react with the specific antibodies.
Smetana
K
K
Likovský
Z
Z
Ochs
R
R
Novák
J
J
Busch
H
H
D002466:Cell Nucleolus / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood* / Q000188:drug therapy / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology*; D011241:Prednisone / Q000627:therapeutic use; D012016:Reference Values
D011241:Prednisone