Dynamics of some genetic marker changes of respiratory-syncytial virus strains circulating among nursery-school children.
Respiratory-syncytial (RS) virus strains circulating during several years appeared polymorphic in respect of two genetic markers: the regression coefficient of infectious activity (RCIA39) characterizing the isolates by their reproduction in tissue cultures at supraoptimal temperature (39 degrees C) and the regression coefficient of neutralization indices (RCNI) characterizing the degree of sensitivity of the strains to antibodies. High-yield RS viruses were more often isolated from children frequently afflicted by the disease, moderate-yield viruses from moderately sick children, while low-yield or none-yield (at 39 degrees C) strains were isolated from rarely afflicted children. On the other hand, RS strains of low reactivity with prototype antibodies were mainly found in often or moderately sick children and the high-reactive ones in rarely sick children. The variability of the RS virus population was continuous, which is consistent with the uninterrupted course of the epidemic process in the nursery-school community. A change of the RCIA39 marker was observed in nearly 50% of strains already after 1 1/2 to 2 months, but most frequently within 5-6 months from the end of disease. The changes of growth intensity at 39 degrees C followed the pattern: high----moderate----low----none, however, in the next epidemic season these properties showed reversion in an opposite direction.
Yurlova
T I
TI
Karpova
L S
LS
Karpukhin
G I
GI
D002460:Cell Line; D002675:Child, Preschool; D006801:Humans; D007668:Kidney; D008168:Lung / Q000196:embryology; D012136:Respiratory Syncytial Viruses / Q000235:genetics* / Q000302:isolation & purification; D010253:Respirovirus Infections / Q000382:microbiology* / Q000503:physiopathology