title: Interactions between trophozoites of Entamoeba histolytica and cells of the immune system.
abstract: The interaction between Entamoeba histolytica trophozoites and cells of the immune system was studied in vitro. Both immune as well as non-immune mononuclear cells (MNC) to some extent were capable of exerting a cytotoxic effect on E. histolytica trophozoites. Incorporation of immune serum directed against the trophozoites in the reaction mixture resulted in a marked increase in the killing capacity of MNC. Highest killing was observed (96.7 +/- 1.04%) with MNC obtained from animals immunized with fraction-I(F-I) of the crude amoebic extract in association with anti-F-I serum. This cytotoxic event was found to be independent of complement involvement and required very low antibody concentrations. This antibody-dependent cellular cytotoxicity against E. histolytica in vitro was found to correlate well with the results of experiments reported earlier by us and others where a high degree of protection has been shown after vaccination of animals with F-I followed by challenge with highly virulent strains of E. histolytica.
authors.forename: S
authors.initials: S
authors.lastname: Chugh
authors.forename: A
authors.initials: A
authors.lastname: Saxena
authors.forename: V K
authors.initials: VK
authors.lastname: Vinayak
mesh_terms: D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D003602:Cytotoxicity, Immunologic; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000276:immunology / Q000469:parasitology; D006168:Guinea Pigs; D007107:Immune System / Q000276:immunology*; D007114:Immunization; D066298:In Vitro Techniques; D013154:Spleen / Q000276:immunology