Induction of an Alu-sequence containing transcript by insulin in human hepatoma cells.
A cDNA clone, designated AF19-1, was isolated from a cDNA library derived from insulin-stimulated hepatoma cells. The nucleotide sequences of AF19-1 showed 83% homology to Alu consensus sequence. It contained a full-length 300-bp Alu family sequence followed in direct tandem by a partial sequence of Alu left monomer. Primer extension analysis confirmed that this Alu transcript was induced even in short-term insulin stimulation. The increase in Alu-transcripts in the early phase of insulin stimulation in hepatoma cells suggests that the Alu sequences may play some important regulatory roles in gene expression.
Chuang
L M
LM
Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, Republic of China.
Lin
B J
BJ
Lee
S C
SC
Tai
T Y
TY
Chen
D S
DS
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D007328:Insulin; D004247:DNA