The genes coding for subunit 3 of NADH dehydrogenase and for ribosomal protein S12 are present in the wheat and maize mitochondrial genomes and are co-transcribed.
A region of about 2 kb which is almost identical in the wheat and maize mitochondrial genomes has been sequenced. It contains a tRNA(Ser) gene, a pseudo-tRNA gene and two open reading frames coding for subunit 3 of the NADH dehydrogenase (118 amino acids) and for ribosomal protein S12 (125 amino acids). The two protein genes are separated by 47 bp and are co-transcribed in wheat and maize. Two transcripts of about 0.9 kb and 3.0 kb, each coding for both proteins, have been characterized, but no monocistronic transcript was detected. Each gene is preceded by a putative ribosome binding site. The pseudo-tRNA gene is interrupted by two insertion sequences in wheat and by one in maize. The origin of the additional interrupting sequence found in the wheat pseudo-tRNA gene, which is also present elsewhere in the mitochondrial genomes, is discussed.
Gualberto
J M
JM
Institut de Biologie Moléculaire des Plantes, Université Louis Pasteur, Strasbourg, France.
Wintz
H
H
Weil
J H
JH
Grienenberger
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial / Q000235:genetics; D005796:Genes; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism; D014158:Transcription, Genetic
D003062:Codon; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D009245:NADH Dehydrogenase