Identification and characterization of glucagon-like peptide-1 7-36 amide-binding sites in the rat brain and lung.
High-affinity binding sites for glucagon-like peptide-1 7-36 amide (GLP-1 7-36 NH2) were identified in rat brain and lung membranes. Binding of [125I]GLP-1 7-36 NH2 was rapid, reversible, specific, saturable and pH dependent. Specific binding in the central nervous system was particularly high in the hypothalamus and the brain stem. Oxyntomodulin, glucagon-like peptide-1, glucagon-like peptide-2 and glucagon were 100-1000-fold less potent than GLP-1 7-36 NH2 in competition for this binding site.
Kanse
S M
SM
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
Kreymann
B
B
Ghatei
M A
MA
Bloom
S R
SR
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D005934:Glucagon / Q000378:metabolism*; D052216:Glucagon-Like Peptide 1; D004763:Glucagon-Like Peptides; D007700:Kinetics; D008168:Lung / Q000378:metabolism*; D009928:Organ Specificity; D010446:Peptide Fragments; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism*; D018027:Receptors, Glucagon
D010446:Peptide Fragments; D010455:Peptides; D011964:Receptors, Gastrointestinal Hormone; D018027:Receptors, Glucagon; C055453:glucagon-like peptide 1 (7-36)amide; D004763:Glucagon-Like Peptides; D052216:Glucagon-Like Peptide 1; D005934:Glucagon