Stimulation of arachidonic acid metabolism by different types of tumor promoters.
Skin tumor-promoting agents, including the 12-O-tetradecanoylphorbol-13-acetate (TPA)-type tumor promoters, such as diterpine phorbol esters, teleocidin and aplysiatoxin, and a non-TPA-type tumor promoter (the newly described palytoxin, present in the coelenterate of the genus Palythoa), stimulated arachidonic acid metabolism by rat liver cells in culture. Palytoxin was 1000-3000 times more effective than TPA-type tumor promoters. The stimulations of arachidonic acid metabolism by palytoxin and the TPA-type tumor promoters were synergistic, whereas the stimulations among the TPA-type tumor promoters were not. The stimulation of arachidonic acid metabolism by palytoxin was synergistic with that of epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha) and transforming growth factor-beta (TGF-beta). An antiserum to the EGF-receptor that blocks EGF binding partially inhibited the synergistic responses to palytoxin and EGF and palytoxin and TGF-alpha, whereas an antiserum to the EGF-receptor that does not block EGF binding or a non-immune rabbit serum did not.
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Fujiki
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D000178:Acrylamides; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002273:Carcinogens; D002460:Cell Line; D003064:Cnidarian Venoms / Q000633:toxicity; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000633:toxicity; D008099:Liver / Q000378:metabolism; D010455:Peptides / Q000633:toxicity; D051381:Rats; D013268:Stimulation, Chemical; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D015291:Transforming Growth Factors; D000095702:Polyether Toxins
D000178:Acrylamides; D016718:Arachidonic Acid; D001095:Arachidonic Acids; D002273:Carcinogens; D003064:Cnidarian Venoms; D004815:Epidermal Growth Factor; D010455:Peptides; D013755:Tetradecanoylphorbol Acetate; D015291:Transforming Growth Factors; C010272:palytoxin; D000095702:Polyether Toxins