Quantification of leukotriene B4 in synovial fluid by gas chromatography/tandem mass spectrometry.
A procedure for the quantification of leukotriene B4 (LTB4) in synovial fluid has been developed using gas chromatography/tandem mass spectrometry based on selected reaction monitoring of the elimination of t-butyldimethylsilanol from the ions of m/z 431 and 438 in the negative ion chemical ionization mass spectra of the di-t-butyldimethylsilyl/pentafluorobenzyl derivatives of leukotriene B4 and the internal standard (2H8)leukotriene B4. The detection limit (approximately 10 pg ml-1) is sufficiently low to permit determination of LTB4 concentration in the synovial fluid of patients with various arthropathies. Single-stage mass spectrometry was found not to be selective enough to permit quantification of LTB4 in synovial fluid.
Dawson
M
M
Department of Rheumatology, Royal North Shore Hospital, St Leonards, NSW, Australia.
McGee
C M
CM
Brooks
P M
PM
Vine
J H
JH
Watson
T R
TR
D001172:Arthritis, Rheumatoid / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis*; D010003:Osteoarthritis / Q000378:metabolism; D013582:Synovial Fluid / Q000032:analysis*
D007975:Leukotriene B4