The inhibitory effects of prednisone, 16-methylen-prednisolone, and ACTH on con-A induced lymphokines (interferon-y) as measured by the chemiluminescence-activity of blood monocytes.
When lymphocytes are stimulated with mitogens or antigens they are enhanced via a cascade of lymphokines to produce interferon-y (IFN-y). IFN-y augments the H2O2 secretion of human monocytes which indirectly can be measured by chemiluminescence. We tested prednisone, 16-methylen-prednisolone and ACTH for their effect to inhibit the Con-A induced stimulation of the chemiluminescence-activity. All three hormones inhibited significantly the stimulation: prednisone up to 52.5% (concentration = 150 micrograms/ml, p = 0.000005), 16-methylen-prednisolone up to 22.5% (concentration = 2.5 micrograms/ml, p = 0.006) and ACTH up to 33% (concentration = 10 micrograms/ml, p = 0.0036).
Hammann
K P
KP
Department of Neurology, Johannes Gutenberg-University, Mainz, F.R.G.
Ruf
P
P
Hopf
H C
HC
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D007371:Interferon-gamma / Q000096:biosynthesis*; D008163:Luminescent Measurements; D009000:Monocytes / Q000276:immunology*; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011241:Prednisone / Q000494:pharmacology*
D003208:Concanavalin A; D007371:Interferon-gamma; D000324:Adrenocorticotropic Hormone; D011239:Prednisolone; D006861:Hydrogen Peroxide; C036266:prednylidene; D011241:Prednisone