Myeloperoxidase secretion during phagocytosis: a case of a patient with impaired bactericidal activity.
We describe a case of a 5-year-old male patient with prolonged and extensive osteomyelitis of the left femur. Staphylococcus aureus was grown from blood cultures taken upon admission and also from pus drained from an incised hip joint. A defect in immune function was suspected and neutrophil function was assessed. Chemotaxis and phagocytosis were normal, but phagocytosed S. aureus were not killed as efficiently as in control neutrophils. No inherent defect in the ability of these neutrophils to generate reactive oxidants was observed, but an unusual luminol-dependent chemiluminescence response was obtained during phagocytosis of latex beads or opsonized S. aureus: This was characterized by an initial rapid, but transient increase occurring within 1 min of addition of phagocytic stimulus. Whereas during phagocytosis of latex beads by control neutrophils less than 1% of the total myeloperoxidase activity was detected extracellularly, up to 15% was released from the patient's neutrophils. We propose that release of myeloperoxidase from the patient's neutrophils during phagocytosis reduces the intraphagosomal concentration of this enzyme and thus impairs the efficiency of intracellular killing of S. aureus.
Edwards
S W
SW
Department of Biochemistry, University of Liverpool, UK.
Say
J E
JE
Taylor
J
J
Hart
C A
CA
D002634:Chemotaxis, Leukocyte; D002675:Child, Preschool; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D010019:Osteomyelitis / Q000201:enzymology / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism*; D010585:Phagocyte Bactericidal Dysfunction / Q000201:enzymology / Q000276:immunology*; D010587:Phagocytosis; D013203:Staphylococcal Infections / Q000201:enzymology / Q000276:immunology; D013481:Superoxides / Q000378:metabolism
D013481:Superoxides; D006861:Hydrogen Peroxide; D009195:Peroxidase