Determination of delta 9-tetrahydrocannabinol in plasma using solid-phase extraction and high-performance liquid chromatography with electrochemical detection.
A method for the determination of nanogram amounts of delta 9-tetrahydrocannabinol (THC) in plasma and serum is described. THC was quantitatively isolated by solid-phase extraction after addition of an aqueous solution of urea and methanol to the sample. The extracts were analysed by high-performance liquid chromatography with electrochemical detection in the oxidizing mode. The detection limit of THC is ca. 100 pg for a signal-to-noise ratio of 3. With this method, levels of 2 ng/ml of THC in plasma can be measured.
Zweipfenning
P G
PG
Forensic Science Laboratory, Rijswijk, The Netherlands.
Lisman
J A
JA
van Haren
A Y
AY
Dijkstra
G R
GR
Holthuis
J J
JJ
D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000097:blood*; D004563:Electrochemistry; D006801:Humans
D013759:Dronabinol