Mutants of translational components that alter reading frame by two steps forward or one step back.
External suppressors, sufS, of a -1 frameshift mutant cause ribosomes to shift into the -1 frame when reading the sequence CAG GGA GUG. The resulting product is not Gln-Gly-Val but Gln-Gly-Ser with Ser being encoded by the underlined AGU. The alleles investigated are approximately 2% efficient in causing frameshifting. Two other suppressors, hopR and hopE of the same -1 frameshift mutant, cause some ribosomes reading the sequence GUG UG to decode a single amino acid, Val, from the five nucleotides. The possibility is considered that peptidyl-tRNA(Val) dissociates from the mRNA, but re-pairs in a triplet manner after the mRNA slips forward by two bases.
Falahee
M B
MB
Howard Hughes Medical Institute, University of Utah, Salt Lake City 84132.
Weiss
R B
RB
O'Connor
M
M
Doonan
S
S
Gesteland
R F
RF
Atkins
J F
JF
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D012270:Ribosomes / Q000378:metabolism*; D013489:Suppression, Genetic
D001426:Bacterial Proteins; D003062:Codon; D012333:RNA, Messenger