title: Formation of hydroxyl radicals in biological systems. Does myoglobin stimulate hydroxyl radical formation from hydrogen peroxide?
abstract: Incubation of horse-heart oxymyoglobin or metmyoglobin with excess H2O2 causes formation of myoglobin(IV), followed by haem degradation. At the time when haem degradation is observed, hydroxyl radicals (.OH) can be detected in the reaction mixture by their ability to degrade the sugar deoxyribose. Detection of hydroxyl radicals can be decreased by transferrin or by .OH scavengers (mannitol, arginine, phenylalanine) but not by urea. Neither transferrin nor any of these scavengers inhibit the haem degradation. It is concluded that intact oxymyoglobin or metmyoglobin molecules do not react with H2O2 to form .OH detectable by deoxyribose, but that H2O2 eventually leads to release of iron ions from the proteins. These released iron ions can react to form .OH outside the protein or close to its surface. Salicylate and the iron chelator desferrioxamine stabilize myoglobin and prevent haem degradation. The biological importance of .OH generated using iron ions released from myoglobin by H2O2 is discussed in relation to myocardial reoxygenation injury.
authors.affiliation: Laboratoire de Biologie Végétale, Université de Nice, France.
authors.forename: A
authors.initials: A
authors.lastname: Puppo
authors.forename: B
authors.initials: B
authors.lastname: Halliwell
mesh_terms: D000818:Animals; D005609:Free Radicals; D006420:Hemeproteins / Q000378:metabolism*; D006736:Horses; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D008786:Metmyoglobin / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D009211:Myoglobin / Q000378:metabolism*; D013053:Spectrophotometry; D013552:Swine; D014168:Transferrin / Q000378:metabolism
chemical_list: D005609:Free Radicals; D006420:Hemeproteins; D006878:Hydroxides; D009211:Myoglobin; D014168:Transferrin; C017595:oxymyoglobin; D008786:Metmyoglobin; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide