N-Acetyl-D-galactosamine-inhibitable adherence lectin of Entamoeba histolytica. II. Mitogenic activity for human lymphocytes.
A soluble protein preparation from virulent axenic amoebae (strain HM1-IMSS) has been demonstrated to be mitogenic for normal human lymphocytes; mitogenesis was optimal at 100 micrograms/ml when incubation was maintained for five to seven days with monocytes (P less than .001). After gel filtration chromatography mitogenic activity of fractions was associated with N-acetyl-D-galactosamine-inhibitable lectin activity (agglutination of Chinese hamster ovary cells; P less than .001). Lymphocyte proliferation induced by these active fractions was specifically inhibited by asialofetuin (P less than .001), which contains three terminal beta 1-4 linked galactose residues. In four strains of axenic amoebae mitogenic activity of soluble protein preparations correlated with in vitro virulence and lectin activity for Chinese hamster ovary cells. The amoebic N-acetyl-D-galactosamine-inhibitable lectin appears to be responsible for the mitogenic activity of soluble amoebic protein preparations; alteration of cell-mediated immunity by this amoebic moiety could potentiate in vivo virulence of the parasite.
Salata
R A
RA
Ravdin
J I
JI
D000116:Acetylgalactosamine / Q000494:pharmacology*; D000268:Adhesiveness; D000328:Adult; D004748:Entamoeba histolytica / Q000276:immunology* / Q000472:pathogenicity; D005688:Galactosamine / Q000031:analogs & derivatives*; D005856:Germ-Free Life; D006801:Humans; D037102:Lectins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000494:pharmacology; D013045:Species Specificity; D014774:Virulence
D037102:Lectins; D011506:Proteins; D005688:Galactosamine; D000116:Acetylgalactosamine