The isolation of a toxin from the dahlia sea anemone, Tealia felina L.
Gel chromatography with Dyematrex blue B gel and ion exchange chromatography with a methylcellulose cation exchange column have been used to isolate a toxin present in partially purified saline extracts of the dahlia sea anemone Tealia felina. Toxin activity was assessed by its histaminolytic activity. A ten-fold increase in activity per mg protein was achieved in these two steps. Final purification on a Sephacryl column yielded an apparently pure protein with a molecular weight of 7800. The amino acid composition of this compound was determined.
Elliott
R C
RC
Konya
R S
RS
Vickneshwara
K
K
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003063:Cnidaria / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008387:Marine Toxins / Q000302:isolation & purification*; D008970:Molecular Weight; D012615:Sea Anemones / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D008387:Marine Toxins