Fine mapping of the immediate-early gene of the Indiana-Funkhauser strain of pseudorabies virus.
The immediate-early gene transcript of pseudorabies virus was found to be 5.6 kilobases and colinear with the DNA genome. Transcription initiation started in the BamHI E fragment, 45 nucleotides from the BamHI-E/BamHI-I junction. The first AUG codon was located in BamHI-I, 260 nucleotides from the mRNA initiation site, and a potential TGA termination codon was located in BamHI-J, 161 nucleotides from the BamHI-I/BamHI-J junction. The AATAAA polyadenylation signal was 30 bases from the stop codon, while the actual polyadenylation site was 18 bases further downstream.
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National Animal Disease Center, U.S. Department of Agriculture, Ames, Iowa 50010.
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004279:DNA, Viral / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D017874:Immediate-Early Proteins; D008969:Molecular Sequence Data; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D017874:Immediate-Early Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D014764:Viral Proteins; C047583:pseudorabies virus immediate early proteins; D015245:Deoxyribonuclease BamHI