Hepatoma-associated alterations of serum alpha 1-antitrypsin in hereditary hepatitis LEC rats as a new animal model of liver disease.
1. The LEC rats, a novel animal model of hepatitis and liver tumor, were found to have charge variants of serum alpha 1-antitrypsin (alpha 1AT) at the stage of liver tumor as judged by isoelectric focusing. 2. Treatment of the sera with neuraminidase showed that acidic variants of alpha 1AT in LEC rats seemed to be highly sialylated forms. 3. The concentration and enzymatic activity of serum alpha 1AT in LEC rats were not affected before the onset of hepatitis and even during the development of fulminant hepatitis and hepatocarcinogenesis. This indicates that hereditary hepatitis and subsequent liver tumor in LEC rats do not appear to be associated with alpha 1AT deficiency.
Masuda
R
R
Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo, Japan.
Sugiyama
T
T
Taniguchi
N
N
Yoshida
M C
MC
Sasaki
M
M
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000150:complications / Q000235:genetics; D004195:Disease Models, Animal; D006505:Hepatitis / Q000097:blood* / Q000150:complications / Q000235:genetics; D007106:Immune Sera; D007525:Isoelectric Focusing; D008113:Liver Neoplasms / Q000097:blood* / Q000150:complications / Q000235:genetics; D009439:Neuraminidase; D051381:Rats; D011922:Rats, Mutant Strains; D000515:alpha 1-Antitrypsin / Q000097:blood*
D007106:Immune Sera; D000515:alpha 1-Antitrypsin; D009439:Neuraminidase