Properties of producer and non-producer clones of a Marek's disease turkey lymphoblastoid cell line.
The MDTC-RP30 lymphoblastoid cell line established from Marek's disease (MD) tumors in turkeys consisted of a heterogeneous population of cells 10 to 25 micron in diameter. Large-cell fractions obtained from a bovine fetal serum gradient had a higher titer of cell-associated MD virus (MDV) than the small-cell fractions. Seven single-cell clones were established from MDTC-RP30 cell line: two consisted of large cells, and the other clones consisted of small cells. Infectious MDV was rescued from large-cell clones in chicken embryo fibroblast cultures but not from small-cell clones. All clones contained MDV DNA sequences when hybridized against cloned MDV DNA. All clones were positive for a Marek's-disease-tumor-associated surface antigen and surface immunoglobulins. All but two small-cell clones caused MD in susceptible chickens. The two large-cell-type clones were uniformly tetraploid, whereas one small-cell clone was diploid and the four others were a mixture of diploid and tetraploid, with an occasional triploid cell. Evidence of translocation involving the male (Z) chromosome and the chromosome #3 was seen in one clone. These results suggest that MDV transforms different subpopulations of lymphocytes.
Nazerian
K
K
U.S. Department of Agriculture, Regional Poultry Research Laboratory, East Lansing, Michigan 48823.
Silva
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RF
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004279:DNA, Viral / Q000032:analysis; D008381:Herpesvirus 2, Gallid / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D008214:Lymphocytes / Q000382:microbiology* / Q000473:pathology; D008380:Marek Disease / Q000382:microbiology* / Q000473:pathology; D011003:Ploidies; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D014178:Translocation, Genetic; D014422:Turkeys
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004279:DNA, Viral; D011947:Receptors, Antigen, B-Cell