[The use of monoclonal antibodies for purification of ribonuclease H from E. coli].
Monoclonal antibodies to E. coli ribonuclease H were obtained from two hybrid clones. Using the monoclonal antibodies two immunosorbents were synthesized for RNase H which have a slight difference in the capacity and do not differ in the conditions of antigen elution. A homogeneous (according to electrophoresis in PAAG) preparation of the enzyme was obtained using the synthesized immunosorbents.
Smolianinov
V V
VV
Kaliuzhnaia
A P
AP
Shekhvatova
G V
GV
Mantsygin
Iu A
IuA
Sviatukhina
N V
NV
D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology*; D007163:Immunosorbent Techniques; D016914:Ribonuclease H
D000911:Antibodies, Monoclonal; D004722:Endoribonucleases; D016914:Ribonuclease H
Ispol'zovanie monoklonal'nykh antitel dlia ochistki ribonukleazy H E. coli.