Prostaglandins do not mediate the inhibitory effects of angiotensins II and III on autonomic neurotransmission in the rabbit vas deferens.
The role of prostaglandins in mediating angiotensin effects on autonomic neurotransmission was assessed. This was accomplished by examining the angiotensin-induced production of prostaglandins (PGE) and suppression of non-adrenergic (purinergic) neurotransmission in the presence and absence of cyclo-oxygenase inhibitors. The vas deferens of the rabbit was utilized for these studies because this preparation has a predominant non-adrenergic neurogenic component. Both angiotensins II and III enhanced PGE production and reduced non-adrenergic neurogenic contractions in a concentration-dependent manner from 1 to 1000 nM. Furthermore, indomethacin (2.8 microM) and eicosatetraynoic acid (10 microM) eliminated the inhibitory effect of the angiotensins on non-adrenergic neurotransmission. However, acetylsalicylic acid (10 microM) prevented the production of PGE in response to the angiotensins but failed to alter the suppression of non-adrenergic neurotransmission. The latter data are inconsistent with the hypothesis that PG's mediate angiotensin-induced depression of non-adrenergic neurotransmission. The mechanism by which indomethacin and eicosatetraynoic acid prevent angiotensin effects on non-adrenergic neurotransmission was not elucidated but previous reports suggest that these agents may act as angiotensin receptor antagonists. All of the cyclo-oxygenase inhibitors examined tended to potentiate angiotensin effects on adrenergic neurotransmission.
Trachte
G J
GJ
Department of Pharmacology, University of Minnesota-Duluth, Medical School, 55812.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000804:Angiotensin II / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000805:Angiotensin III / Q000494:pharmacology*; D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001341:Autonomic Nervous System / Q000187:drug effects*; D004357:Drug Synergism; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D011458:Prostaglandins E / Q000494:pharmacology*; D011817:Rabbits; D009435:Synaptic Transmission / Q000187:drug effects*; D014649:Vas Deferens / Q000294:innervation*
D011458:Prostaglandins E; D000804:Angiotensin II; D015117:5,8,11,14-Eicosatetraynoic Acid; D000805:Angiotensin III; D001241:Aspirin; D007213:Indomethacin