A monoclonal antibody stains blastemal but not tubular components of Wilms' tumour.
The monoclonal antibody PAL-E is specific for endothelial cells in a wide variety of normal and tumour tissue. In normal kidney, PAL-E reacts exclusively with the endothelium of non-glomerular blood vessels. In Wilms' tumour, binding of PAL-E was not restricted to the endothelium; staining of blastemal cells was observed in seven out of eight cases examined. Mesenchymal and tubular components, if present in Wilms' tumour, were negative. In contrast, a monoclonal antibody to Factor VIII-related antigen (RFF-8-R-1) bound only to endothelial cells in these tumours. In fetal kidney, PAL-E binding showed a wider distribution than in adult kidney and both stromal and glomerular capillaries were stained. Tubules and non-endothelial stromal cells were negative. These results indicate that the reactivity of the monoclonal antibody, PAL-E, is not restricted to cells of endothelial origin in all tissues. The implications of these findings for the differentiation of Wilms' tumour are discussed.
Sarawar
S R
SR
Clinical Research Laboratories, Christie Hospital, Manchester, U.K.
Schlingemann
R O
RO
Kelsey
A
A
Fleming
S
S
Kumar
S
S
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000276:immunology; D004730:Endothelium, Vascular / Q000473:pathology; D005169:Factor VIII / Q000276:immunology; D005333:Fetus; D006801:Humans; D007668:Kidney / Q000473:pathology; D007678:Kidney Glomerulus / Q000473:pathology; D007680:Kidney Neoplasms / Q000473:pathology*; D007684:Kidney Tubules / Q000473:pathology; D009396:Wilms Tumor / Q000473:pathology*; D014841:von Willebrand Factor
D000911:Antibodies, Monoclonal; D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII