In vitro expression of the alpha and beta subunits of the Na,K-ATPase.
Our analysis of the cloned alpha 3 protein strongly suggests that this c-DNA represents a bona fide Na+,K+ ATPase isoform. Its similarity to alpha + may have made its detection in tissues by gel migration or immunoreactivity difficult. Expression of an enzymatically active alpha 3 beta Na+K+ATPase either in a completely in vitro system or in a heterologous tissue culture system will clearly establish the biochemical properties of this isoform. Development of alpha 3 specific immunochemical probes will allow a proper assessment of its in vivo expression.
Gilmore-Hebert
M
M
Department of Internal Medicine, Yale University School of Medicine, New Haven CT.
Mercer
R W
RW
Schneider
J W
JW
Benz
E J
EJ
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D003001:Cloning, Molecular; D007527:Isoenzymes / Q000235:genetics; D046911:Macromolecular Substances; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D013698:Templates, Genetic
D007527:Isoenzymes; D046911:Macromolecular Substances; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase