Characterization of a highly repeated satellite DNA from the cyprinid fish Notropis lutrensis.
1. A highly repeated, satellite DNA family from the North American cyprinid fish, Notropis lutrensis, was identified as a fragment band following restriction endonuclease enzyme digestion and agarose gel electrophoresis of genomic DNA; evidence of a tandem arrangement of the satellite in the genome was demonstrated by the formation of "ladders" in partial restriction endonuclease digests. 2. The satellite family was estimated densitometrically to comprise 7-8% of the N. lutrensis genome; mapping experiments using isolated and purified monomer repeat units of the satellite uncovered nine sites for seven different restriction enzymes. 3. A monomeric repeat unit of the satellite was cloned and sequenced, and found to be 174 base pairs in length and to have a base composition of 47% G + C (guanine + cytosine); computer analysis of the sequence revealed 13 new restriction sites for 12 additional enzymes. 4. Computer analysis also revealed that a large degree of internal redundancy in the monomer unit exists in the form of both direct and inverted repeating units, and that the entire sequence, starting with one base in either orientation, constitutes an open reading frame. In all but the last characteristic, the N. lutrensis satellite DNA is very similar to satellite DNAs in other eukaryotes.
Moyer
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Department of Wildlife and Fisheries Sciences, Texas A & M University, College Station 77843.
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Thomas
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Gold
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D000818:Animals; D001483:Base Sequence; D003530:Cyprinidae / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004276:DNA, Satellite / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004276:DNA, Satellite; D004262:DNA Restriction Enzymes