title: Allosteric transition of erythrocyte alkaline phosphatase from Duchenne muscular dystrophy (DMD) patients and Duchenne muscular dystrophy carriers (Homo sapiens).
abstract: 1. The kinetic properties of the p-nitrophenylphosphatase (EC 3.1.3.1) from erythrocytes was investigated in DMD-patients and DMD-carriers. 2. A different allosteric behaviour in the p-nitrophenylphosphatase from DMD-patients and DMD-carriers compared to controls is supported by the following findings: (a) values of n altered in F- inhibition of (K+)-activated p-nitrophenylphosphatase with Hill coefficients -1.5, -2.2 and -3.1; (b) heterotropic effect of increased concentration of Mg2+ on F- inhibition which is reverted by K+ in DMD-carriers and in control, but not in DMD-patients. 3. Evidence is presented showing that in DMD-patients and in DMD-carriers the interaction membrane-enzyme is different from the corresponding controls.
authors.affiliation: Facultad de Ciencias Exactas y Naturales, U.N. de Mar del Plata, Argentina.
authors.forename: A L
authors.initials: AL
authors.lastname: Goldemberg
authors.forename: A M
authors.initials: AM
authors.lastname: García
authors.forename: H
authors.initials: H
authors.lastname: Fernández
authors.forename: M
authors.initials: M
authors.lastname: Fortunato
authors.forename: J J
authors.initials: JJ
authors.lastname: Sánchez
authors.forename: R E
authors.initials: RE
authors.lastname: Trucco
mesh_terms: D009597:4-Nitrophenylphosphatase / Q000037:antagonists & inhibitors / Q000097:blood*; D000469:Alkaline Phosphatase / Q000097:blood*; D000494:Allosteric Regulation; D004910:Erythrocyte Membrane / Q000201:enzymology* / Q000502:physiology; D005260:Female; D005459:Fluorides / Q000494:pharmacology; D006579:Heterozygote; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000502:physiology; D008297:Male; D009136:Muscular Dystrophies / Q000097:blood / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000097:blood*
chemical_list: D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase; D008274:Magnesium; D005459:Fluorides