Isolation and propagation of hepatitis A virus in hepatoma cell cultures.
Hepatitis A virus (HAV) was isolated directly from human faeces in PLC/PRF/5 cells. In the first passage cell-bound and supernatant viruses were found by immune electron microscopy and by enzymeimmunoassay. Serial passaging of HAV in PLC/PRF/5 cells resulted in its adaptation to the cell line and in reduction of the incubation time. HAV was still detectable after 10 cell passages. Cell-bound as well as supernatant HAV were employed as antigens in anti HAV IgM-enzymeimmunoassay.
Friedman-Alvermann
B
B
Müller
D
D
Spies
K
K
D000328:Adult; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D005243:Feces / Q000382:microbiology; D005260:Female; D006506:Hepatitis A / Q000382:microbiology; D035922:Hepatitis A Antibodies; D035921:Hepatitis A Antigens; D006508:Hepatitis Antibodies / Q000032:analysis; D006507:Hepatovirus / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis; D008113:Liver Neoplasms; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D014776:Virus Cultivation
D000956:Antigens, Viral; D035922:Hepatitis A Antibodies; D035921:Hepatitis A Antigens; D006508:Hepatitis Antibodies; D007075:Immunoglobulin M