title: Phenotypic and genotypic heterogeneity of peripheral T-cell lymphoma.
abstract: A series of 21 phenotypically characterised T-cell lymphomas histologically defined as lymphocytic, lymphoblastic, immunoblastic, AILD type, pleomorphic, T-zone and Lennert's T-cell lymphoma, were investigated for T-cell receptor (TcR) and immunoglobulin (Ig) gene rearrangements. Phenotypic analyses of frozen sections and cell suspensions were heterogeneous and in many cases no single T-cell marker recognised all of the malignant cells. Data derived by staining with antibodies reactive with antigens in paraffin embedded tissue were consistent with T NHL in all cases except lymphoblastic lymphoma. TcR gene rearrangements were observed in lymphocytic, lymphoblastic and immunoblastic lymphoma, however, in the remaining 14 phenotypically and histologically defined peripheral T-cell lymphomas, 2 showed rearrangement of TcR gamma and beta genes consistent with T NHL and 2 showed Ig JH rearrangements only, suggestive of either reactive T-cell populations masking cryptic disease or presence of tumour populations with aberrant gene rearrangement and expression of T lineage antigens. No Ig or TcR gene rearrangements were found in the remaining 10 cases, in which morphologically identifiable tumour cells comprised 10-90% of the cell population. In 3/6 cases tested some CD3 positive cells failed to stain with WT31 or beta F1, monoclonal antibodies that recognise determinants on combined TcR gamma beta or TcR beta chains respectively. Whether these cases represent tumours arising from an undetermined cell of origin or polyclonal expansions of T-cells remains to be determined. Our results confirm the phenotypic heterogeneity of histologically defined peripheral T-cell lymphoma and indicate that in these particular histological subtypes gene rearrangement analysis can also yield heterogeneous results which may be unhelpful in determining cell lineage and clonality.
authors.affiliation: Regional Immunology Service, Southampton General Hospital.
authors.forename: J L
authors.initials: JL
authors.lastname: Smith
authors.forename: D G
authors.initials: DG
authors.lastname: Haegert
authors.forename: E
authors.initials: E
authors.lastname: Hodges
authors.forename: G N
authors.initials: GN
authors.lastname: Stacey
authors.forename: W M
authors.initials: WM
authors.lastname: Howell
authors.forename: D H
authors.initials: DH
authors.lastname: Wright
authors.forename: D B
authors.initials: DB
authors.lastname: Jones
mesh_terms: D015342:DNA Probes; D004273:DNA, Neoplasm; D015321:Gene Rearrangement; D015329:Gene Rearrangement, T-Lymphocyte; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D005803:Genes, Immunoglobulin; D005838:Genotype; D006801:Humans; D008223:Lymphoma / Q000235:genetics / Q000473:pathology*; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell; D013601:T-Lymphocytes
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm; D011948:Receptors, Antigen, T-Cell