title: Nucleotide phosphotransferase, nucleotide kinase and inorganic pyrophosphatase activities of killer virions of yeast.
abstract: The intracellular killer virions of yeast co-purify with an RNA polymerase activity which catalyzes the synthesis of full-length transcripts of the two viral genomic double-stranded RNA segments. This polymerase utilizes ribonucleoside diphosphates or triphosphates as substrates. The virions have other associated nucleotide-metabolizing enzyme activities, including nucleoside diphosphate kinase, adenosine monophosphate kinase, and nucleoside triphosphate phosphotransferase, an activity which catalyzes the exchange of gamma-phosphate from any ribonucleoside triphosphate with any ribonucleoside or deoxyribonucleoside triphosphate. The purified virions also contain an inorganic pyrophosphatase activity. These enzymes may allow the virus to utilize nucleotide pools distinct from those utilized in host cell transcription.
authors.affiliation: Department of Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854.
authors.forename: D E
authors.initials: DE
authors.lastname: Georgopoulos
authors.forename: M J
authors.initials: MJ
authors.lastname: Leibowitz
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003570:Cytidine Triphosphate / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006358:Hot Temperature; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism*; D012328:RNA Viruses / Q000201:enzymology*; D012441:Saccharomyces cerevisiae; D014530:Uridine Diphosphate / Q000378:metabolism; D014771:Virion / Q000201:enzymology*
chemical_list: D006153:Guanosine Diphosphate; D014530:Uridine Diphosphate; D000244:Adenosine Diphosphate; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D010770:Phosphotransferases; C021898:nucleotide phosphotransferase; D009701:Nucleoside-Diphosphate Kinase; D012321:DNA-Directed RNA Polymerases; D009708:Nucleotidases; C018775:adenosine monophosphatase; D011755:Pyrophosphatases