Hepatocyte cell surface polarity as demonstrated by lectin binding.
We performed an investigation at the ultrastructural level of the differential distribution of lectin-binding sites among sinusoidal, lateral, and bile canalicular domains of adult rat hepatocytes. Lectin binding to hepatocyte glycocalices was studied in situ or after cellular dissociation by enzymatic (collagenase), chemical (EDTA), and mechanical methods, as well as during cell culture. Using thirteen biotinylated lectins and an avidin-biotin-peroxidase complex (ABC), we have identified lectin-binding sites that are predominantly localized in the bile canalicular [Ricinus communis agglutinin (RCA)] or sinusoidal [Phaseolus vulgaris (PHA)] domains in situ and in mechanically dissociated cells. Lens culinaris (LCA) staining was prominent on sinusoidal surfaces, slight along lateral surfaces, and completely absent in the bile canalicular domain. Concanavalin A (ConA) was unique in binding equally to all domains. Triticum vulgaris [wheat germ agglutinin (WGA)] was also bound to all domains, but most intensely to the bile canalicular region. Cells dissociated via collagenase or EDTA treatment exhibited a spherical morphology characterized by many surface microvilli and absence of morphological domains. Lectin binding to dissociated cells was uniformly distributed over the entire cell surface, suggesting a redistribution of lectin receptors that was independent of the separation procedure. Hepatocytes in culture exhibited a partial restoration of morphological domains, but lectin binding polarity was not re-established.
McMillan
P N
PN
Department of Pathology, Rhode Island Hospital, Providence 02903.
Hixson
D C
DC
Hevey
K A
KA
Naik
S
S
Jauregui
H O
HO
D000818:Animals; D001360:Avidin; D001648:Bile Canaliculi / Q000378:metabolism / Q000648:ultrastructure; D001665:Binding Sites; D001710:Biotin; D002478:Cells, Cultured; D003208:Concanavalin A / Q000378:metabolism; D004492:Edetic Acid; D037102:Lectins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D003012:Microbial Collagenase; D008854:Microscopy, Electron; D037121:Plant Lectins; D051381:Rats; D011919:Rats, Inbred Strains; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014909:Wheat Germ Agglutinins; C033619:lentil lectin; D003208:Concanavalin A; D001360:Avidin; D001710:Biotin; D004492:Edetic Acid; D003012:Microbial Collagenase