title: Isolation and in vitro translation of Onchocerca volvulus mRNA.
abstract: Adult Onchocerca volvulus recovered for excised nodules by dissection or treatment with collagenase have been used as a source of RNA for in vitro translation experiments. RNA was purified using either the hot phenol/SDS procedure or the guanidine isothiocyanate protocol. Immunoprecipitation experiments performed on in vitro products demonstrate a marked heterogeneity in responses by individed human infection sera. Further immunoprecipitation experiments demonstrate cross reactivity between O. volvulus and other filarial nematodes.
authors.affiliation: Department of Pathology, University of Cambridge, U.K.
authors.forename: V M
authors.initials: VM
authors.lastname: Connor
authors.forename: G
authors.initials: G
authors.lastname: Braun
authors.forename: F
authors.initials: F
authors.lastname: Engelbrecht
authors.forename: J Y
authors.initials: JY
authors.lastname: Cesbron
authors.forename: M J
authors.initials: MJ
authors.lastname: Downham
authors.forename: J
authors.initials: J
authors.lastname: Whitworth
authors.forename: J E
authors.initials: JE
authors.lastname: McMahon
authors.forename: D W
authors.initials: DW
authors.lastname: Taylor
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D003012:Microbial Collagenase; D009854:Onchocerca / Q000235:genetics* / Q000276:immunology; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000947:Antigens, Helminth; D007106:Immune Sera; D012333:RNA, Messenger; D003012:Microbial Collagenase