title: Three-dimensional structure of the ribonuclease T1 2'-GMP complex at 1.9-A resolution.
abstract: The complex formed between the enzyme ribonuclease T1 (EC 3.1.27.3) and its specific inhibitor 2'-guanylic acid (2'-GMP) has been refined to R = 0.180 using x-ray diffraction data to 1.9-A resolution. The protein molecule displays a compact fold; a 4.5 turn alpha-helix packed over an antiparallel beta-pleated sheet shields most of the hydrophobic interior of the protein against the solvent. The extended pleated sheet structure of ribonuclease T1 is composed of three long and four short strands building up a two-stranded minor beta-sheet near the amino terminus and a five-stranded major sheet in the interior of the protein molecule. In the complex with ribonuclease T1, the inhibitor 2'-guanylic acid adopts the syn-conformation and C2'-endo sugar pucker. Binding of the nucleotide is mainly achieved through amino acid residues 38-46 of the protein. The catalytically active amino acid residues of ribonuclease T1 (His40, Glu58, Arg77, and His92) are located within the major beta-sheet which, as evident from the analysis of atomic temperature factors, provides an environment of minimal local mobility. The geometry of the active site is consistent with a mechanism for phosphodiester hydrolysis where, in the transesterification step, His40 and/or Glu58 act as a general base toward the ribose 2'-hydroxyl group and His92, as a general acid, donates a proton to the leaving 5'-hydroxyl group.
authors.affiliation: Institut für Kristallographie, Freie Universität Berlin, West Germany.
authors.forename: R
authors.initials: R
authors.lastname: Arni
authors.forename: U
authors.initials: U
authors.lastname: Heinemann
authors.forename: R
authors.initials: R
authors.lastname: Tokuoka
authors.forename: W
authors.initials: W
authors.lastname: Saenger
mesh_terms: D004722:Endoribonucleases / Q000378:metabolism*; D006157:Guanosine Monophosphate / Q000378:metabolism; D006860:Hydrogen Bonding; D008958:Models, Molecular; D011487:Protein Conformation; D006163:Ribonuclease T1 / Q000378:metabolism*
chemical_list: C026693:2'-guanylic acid; D006157:Guanosine Monophosphate; D004722:Endoribonucleases; D006163:Ribonuclease T1