Fetal liver transplantation in the mini-pig.
After the characterisation of the hematological and immunological status of the mini-pig fetus at different gestational ages of development was performed, two different groups of animals receiving 800 rads of TBI given by a radioactive cobalt source at a dose/rate of 5/6 rad/min or 750 rads of TBI given by a Linear Accelerator at a dose/rate of 25/26 rad/min, both in a single dose exposure, were transplanted with a pool of allogeneic fetal liver cells whose age ranged between 55 and 75 days of gestation. In the first group 1 animal out of 6 is alive and well 30 months post-transplant. In the second group one of the nine transplanted animals survived 78 days. Engraftment was proved by the presence of the donor chromosome in the proliferating bone marrow cells in one animal.
Andreani
M
M
De Biagi
M
M
Centis
F
F
Manna
M
M
Agostinelli
F
F
Filippetti
A
A
Gaudenzi
G
G
Muretto
P
P
Grianti
C
C
Sotti
G
G
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007136:Immunoglobulins / Q000032:analysis; D007621:Karyotyping; D008099:Liver / Q000196:embryology / Q000276:immunology; D016031:Liver Transplantation; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D011247:Pregnancy; D012397:Rosette Formation; D013552:Swine; D013556:Swine, Miniature
D007136:Immunoglobulins