[Use of reconstructed Sendai virus envelopes for the microinjection of proteins into monolayer cells].
It has been shown that biological microinjection, that is, fusion of protein-charged human erythrocytes with monolayer cells may be achieved by means of reconstructed Sendai virus consisting of envelope structures but devoid of nucleoid. Sendai virion envelopes reconstructed by dialysis possessed no infectivity, interferon-inducing, or interfering activities. The use of 125I-IgG demonstrated that erythrocytes are loaded with protein by 35%-40% and that each of the monolayer L929 cells is fused on the average with 4 loaded erythrocytes.
Bokhon'ko
A I
AI
Zhdanov
L V
LV
Orlova
T G
TG
D000818:Animals; D002459:Cell Fusion; D003956:Dialysis / Q000379:methods; D004912:Erythrocytes / Q000382:microbiology; D006801:Humans; D007457:Iodine Radioisotopes; D007739:L Cells; D051379:Mice; D008845:Microinjections / Q000379:methods*; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects / Q000472:pathogenicity*; D014759:Viral Envelope Proteins / Q000008:administration & dosage* / Q000302:isolation & purification; D014771:Virion / Q000187:drug effects / Q000472:pathogenicity
D007457:Iodine Radioisotopes; D014759:Viral Envelope Proteins
Ispol'zovanie rekonstruirovannykh obolochek virusa Sendaĭ dlia mikroin''etsirovaniia belkov v kletki monosloia.