Differences in c-AMP levels in epithelial cells from pelvic and pectoral regions of the toad skin.
Arginine vasopressin (AVP) stimulated active Na+ transport (JNa+) and osmotic water flow (JH2O) across the pelvic skin but only JNa+ across the pectoral skin of the toad, Bufo woodhouseii. Isolated epithelial cells from the pelvic skin had a maximal c-AMP level of 11.16 pmoles/mg protein after 5 min of AVP treatment while that of pectoral skin was 3.64 pmoles/mg protein. The c-AMP level of both skin areas fell to unstimulated values after 20 min of AVP treatment; however, JH2O (pelvic skin) and JNa+ (pelvic and pectoral skin) remained elevated during 3 hr of treatment. Dibutyryl c-AMP and theophylline stimulated JH2O across the pelvic but not the pectoral skin. Maintaining toads in water for 12-24 hr resulted in a substantial lowering of JH2O across the pectoral skin which was not reversible by treatment with c-AMP and theophylline.
Marrero
M B
MB
Hillyard
S D
SD
D000818:Animals; D002025:Bufonidae / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D010539:Permeability; D012867:Skin / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology; D014882:Water-Electrolyte Balance
D012964:Sodium; D013806:Theophylline; D000242:Cyclic AMP