title: Nucleic acid spot hybridization with nonradioactive labeled probes in screening for human papillomavirus DNA sequences.
abstract: We examined 161 human tissue samples using the spot hybridization technique with nonradioactive labeled DNA probes of human papillomavirus (HPV). Whole cells were spotted on nitrocellulose filters; DNA of the cells was denatured and fixed to the filter. Then the DNA spots were hybridized to nonradioactive labeled DNA and monitored by a sandwich immunoenzymatic reaction. This technique is simple, sensitive, specific, requires no special equipment, and can be used in clinical settings. HPV DNA was found in 92% of samples in which, on the basis of histologic and colposcopic criteria, its presence was suspected, as well as in 31 samples where it was not suspected.
authors.affiliation: Department of Microbiology and Immunology, Institut Alfred Fournier, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Melki
authors.forename: B
authors.initials: B
authors.lastname: Khoury
authors.forename: F
authors.initials: F
authors.lastname: Catalan
mesh_terms: D002584:Cervix Uteri / Q000382:microbiology; D015342:DNA Probes; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D011237:Predictive Value of Tests; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002577:Uterine Cervical Diseases / Q000175:diagnosis*
chemical_list: D015342:DNA Probes; D015344:DNA Probes, HPV; D004279:DNA, Viral