Role of inositol starvation on ecto-5'-nucleotidase activity during mitogen-induced lymphocyte activation.
Cell-surface 5'-nucleotidase was assayed by incubating intact cells with 5' [3H]AMP in iso-osmotic buffer and measuring [3H]adenosine production. The activity of cell surface 5'-nucleotidase in small resting lymphocytes and in cells of the B cell line BCL1 was 5.7 and 1.1 nmol/min/mg protein respectively at 37 degrees C. The 5'-nucleotidase was inhibited by Concanavalin A and anti-IgM, the inhibition by anti-IgM being reversible. Incubating the lymphocytes in the presence and absence of mitogens in inositol-free medium for 15 min, 60 min, and 24 h had no effect on 5'-nucleotidase activity. The reaction product adenosine as well as adenine nucleotides were shown to inhibit mitogen-induced proliferation.
Hauschildt
S
S
Institut für Immunbiologie der Universität Freiburg, F.R.G.
Bessler
W G
WG
D015720:5'-Nucleotidase; D000227:Adenine Nucleotides / Q000494:pharmacology; D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D066298:In Vitro Techniques; D007294:Inositol / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D009708:Nucleotidases / Q000378:metabolism*
D000227:Adenine Nucleotides; D008934:Mitogens; D007294:Inositol; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000241:Adenosine