Dihydroorotic acid dehydrogenase activity in actinomycin-D-treated and normal chick embryos.
The effect of actinomycin D on chick embryos cultivated in vitro by New's culturing method was studied. Exposure of chick embryos to actinomycin D (0.05 micrograms/ml) at the primitive streak stage (stage 4; Hamburger and Hamilton) for 6 h showed interference in orotic acid formation. The assay of the enzyme dihydroorotic acid dehydrogenase was carried out in both treated and control embryos. No enzymic activity was observed in actinomycin-D-treated embryos in contrast to the considerable activity in the controls. These observations suggest an interference by actinomycin D in the biogenesis of the enzyme dihydroorotic acid dehydrogenase.
Katdare
S M
SM
Department of Zoology, University of Poona, Pune, India.
Katdare
M
M
Joshi
P N
PN
Mulherkar
L
L
D000818:Animals; D002642:Chick Embryo / Q000201:enzymology*; D003609:Dactinomycin / Q000494:pharmacology*; D004081:Dihydroorotate Oxidase / Q000096:biosynthesis / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*
D003609:Dactinomycin; D010088:Oxidoreductases; D004081:Dihydroorotate Oxidase