Activation of the release of dopamine in the carotid body by veratridine. Evidence for the presence of voltage-dependent Na+ channels in type I cells.
Veratridine (50 microM), an agent known to activate voltage-dependent Na+ channels, induced a strong release of [3H]dopamine from the rabbit carotid body in vitro. The effect of veratridine was dependent on the presence of both Na+ and Ca2+ in the extracellular medium and was abolished by 1 microM tetrodotoxin. These results suggest that chemoreceptor type I cells have voltage-dependent Na+ channels, which could be involved in the depolarization of the cell membrane and activation of voltage-dependent Ca2+ channels.
Rocher
A
A
Departamento de Bioquimica, Facultad de Medicina, Universidad de Valladolid, Spain.
Obeso
A
A
Herreros
B
B
Gonzalez
C
C
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002344:Carotid Body / Q000166:cytology / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D018492:Dopamine Antagonists; D066298:In Vitro Techniques; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology*; D014702:Veratrine / Q000031:analogs & derivatives*
D018492:Dopamine Antagonists; D015222:Sodium Channels; D013779:Tetrodotoxin; D014701:Veratridine; D012964:Sodium; D014702:Veratrine; D002118:Calcium; D004298:Dopamine