title: A dot enzyme-linked immunosorbent assay for detection of antibodies against Entamoeba histolytica.
abstract: A visual micro-dot enzyme-linked immunosorbent assay (ELISA) based on detection of antibodies against soluble antigens of axenically grown cultures of Entamoeba histolytica is described. The antigen was spotted on a nitrocellulose sheet, the unsaturated sites blocked with bovine serum albumin (BSA) and incubated with 3-fold dilutions of patient sera followed by incubation with protein A conjugated to peroxidase. Enzymic activity was evidenced using the substrate 4-chloro-1-naphthol. A positive reaction produced a blue spot. The sensitivity of the assay was better and comparable to the indirect haemagglutination assay (IHA) and plate ELISA. The entire assay could be completed within 3 h. Antigen-loaded and pre-blocked nitrocellulose strips could be stored up to 3 months at room temperature and 37 degrees C.
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: A H
authors.initials: AH
authors.lastname: Band
authors.forename: J C
authors.initials: JC
authors.lastname: Samantaray
authors.forename: N
authors.initials: N
authors.lastname: Dang
authors.forename: G P
authors.initials: GP
authors.lastname: Talwar
mesh_terms: D000562:Amebiasis / Q000175:diagnosis*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D004306:Dose-Response Relationship, Immunologic; D004748:Entamoeba histolytica / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*
chemical_list: D000906:Antibodies