Isolation from porcine intestinal extracts of a cholecystokinin-like peptide and a peptide with homology to cytochrome oxidase polypeptide VII and chymodenin.
A number of different forms of cholecystokinin (CCK) exist in the brain and intestine. Gel permeation and ion exchange chromatography and high performance liquid chromatography have been used to isolate a peptide from partially purified porcine intestinal extracts with N-terminal homology to porcine brain CCK-58. This peptide contracted both the guinea pig ileum longitudinal muscle and gallbladder muscle and these responses were inhibited by dibutyryl cyclic GMP or proglumide. The potency was approximately 1/100 of that of CCK-8. The reason for this low potency is unclear, but it is possible that a critical part of the biologically active region is modified or that it is a truncated form of CCK-58. A further peptide was isolated with a sequence homologous to cytochrome oxidase polypeptide VII and chymodenin.
McIntosh
C H
CH
MRC Regulatory Peptide Group, Department of Physiology, University of British Columbia, Vancouver, Canada.
Dahl
M A
MA
Kwok
Y N
YN
Mutt
V
V
Spruston
N
N
Brown
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002766:Cholecystokinin / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003576:Electron Transport Complex IV / Q000302:isolation & purification*; D005260:Female; D006168:Guinea Pigs; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid
D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D010455:Peptides; C007058:chymodenin; D002766:Cholecystokinin; D003576:Electron Transport Complex IV