IS1-mediated mobility of the aerobactin system of pColV-K30 in Escherichia coli.
Genes determining the high affinity iron transport system mediated by the siderophore aerobactin are flanked in the enterobacterial plasmid pColV-K30 by inverted repeats of IS1 sequences, suggesting that the aerobactin genes are part of a transposon. To study this possibility, the entire region between the two IS1 sequences was cloned as an 18 kb HindIII-BamHI restriction fragment in pUC8 giving plasmid pMO1. A number of derivatives of pMO1, in which aerobactin genes were tagged with a kanamycin resistance gene, were prepared in order to assess the ability of both IS1s to promote the formation of cointegrates with pCJ105, an F derivative devoid of insertion sequences. Mating-out assays indicated that both flanking IS1s were active in cointegrate formation at detectable frequencies. In some cases, the cointegrates could be resolved, the final result being a transposition-like event for the entire aerobactin system.
de Lorenzo
V
V
Department of Biochemistry, University of California, Berkeley 94720.
Herrero
M
M
Neilands
J B
JB
D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D006877:Hydroxamic Acids / Q000378:metabolism*; D010957:Plasmids; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D006877:Hydroxamic Acids; C031819:aerobactin