Neurotransmitter systems in morphologically undifferentiated human Y-79 retinoblastoma cells: studies of GABAergic, glycinergic, and beta-adrenergic systems.
Elements of three neurotransmitter systems were investigated in morphologically undifferentiated human Y-79 retinoblastoma cells in suspension culture. Specific gamma-aminobutyric acid (GABA) uptake, GABA binding, and glycine binding were absent from these cells, although the cells had been shown to exhibit an active uptake and release of [3H]glycine. Binding and competition studies using both alpha- and beta-adrenergic ligands indicated the presence of a beta-adrenergic receptor. This finding was confirmed by treatment of the cells with beta-agonists in competition with a beta-antagonist and with an alpha-antagonist; the level of cyclic AMP was competitively stimulated. Therefore, human Y-79 cells in suspension culture contain beta-adrenergic receptors, and not glycinergic or GABAergic ones. Thus, the Y-79 cells may be of use in studying the factors involved in developmental regulation of neurotransmitter function.
Madtes
P
P
Kyritsis
A
A
Chader
G J
GJ
D000318:Adrenergic beta-Agonists / Q000378:metabolism; D000319:Adrenergic beta-Antagonists / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000032:analysis; D005134:Eye Neoplasms / Q000032:analysis / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism*; D006801:Humans; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011898:Ranidae; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012175:Retinoblastoma / Q000032:analysis / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D018377:Neurotransmitter Agents; D011943:Receptors, Adrenergic, beta; D005680:gamma-Aminobutyric Acid; D000242:Cyclic AMP; D005998:Glycine