title: Subunit location of the iron-sulfur clusters in fumarate reductase from Escherichia coli.
abstract: The subunit location of the [2Fe-2S], [3Fe-4S], and [4Fe-4S] clusters in Escherichia coli fumarate reductase has been investigated by EPR studies of whole cells or whole cells extracts of a fumarate reductase deletion mutant with plasmid amplified expression of discrete fumarate reductase subunits or groups of subunits. The results indicate that both the [2Fe-2S] and [3Fe-4S] clusters are located entirely in the iron-sulfur protein subunit. Information concerning the specific cysteine residues that ligate these clusters has been obtained by investigating the EPR characteristics of cells of the deletion mutant amplified with a plasmid coding for the flavoprotein subunit and a truncated iron-sulfur protein subunit. While the results are not definitive with respect to the location of the [4Fe-4S] cluster, they are most readily interpreted in terms of this cluster being entirely in the flavoprotein subunit or bridging between the two catalytic domain subunits. These new results are discussed in light of the amino acid sequences of the two subunits and the sequences of structurally well characterized iron-sulfur proteins containing [2Fe-2S], [3Fe-4S], and [4Fe-4S] centers.
authors.affiliation: School of Chemical Sciences, University of Georgia, Athens 30602.
authors.forename: M K
authors.initials: MK
authors.lastname: Johnson
authors.forename: A T
authors.initials: AT
authors.lastname: Kowal
authors.forename: J E
authors.initials: JE
authors.lastname: Morningstar
authors.forename: M E
authors.initials: ME
authors.lastname: Oliver
authors.forename: K
authors.initials: K
authors.lastname: Whittaker
authors.forename: R P
authors.initials: RP
authors.lastname: Gunsalus
authors.forename: B A
authors.initials: BA
authors.lastname: Ackrell
authors.forename: G
authors.initials: G
authors.lastname: Cecchini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D002417:Cattle; D003001:Cloning, Molecular; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D007501:Iron / Q000032:analysis; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D046911:Macromolecular Substances; D008667:Metalloproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D010957:Plasmids; D013045:Species Specificity; D013385:Succinate Dehydrogenase / Q000235:genetics*; D013455:Sulfur / Q000032:analysis
chemical_list: D007506:Iron-Sulfur Proteins; D046911:Macromolecular Substances; D008667:Metalloproteins; D013455:Sulfur; D007501:Iron; D013385:Succinate Dehydrogenase