Inhibition of stimulated cell responses by phorbol esters and other activators of protein kinase C: sites of action.
The addition of the protein kinase C activator phorbol esters to cell suspension a few minutes prior to stimulation inhibits the agonists-induced biochemical changes and cell responses. This inhibition is prevented by protein kinase C inhibitors. Activation of protein kinase C down regulates the stimulated responses by affecting one or more of the steps in the exitation-response coupling. This includes the receptors, the quanine-nucleotide-binding protein, the activity or distribution of phospholipase C, and other steps.
Sha'afi
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RI
Department of Physiology, University of Connecticut Health Center, Farmington 06032.
Molski
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TF
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D010703:Phorbol Esters / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011817:Rabbits; D015398:Signal Transduction
D010703:Phorbol Esters; D010716:Phosphatidylinositols; D011493:Protein Kinase C; D019204:GTP-Binding Proteins; D002118:Calcium