title: Cholinergic stimulation of chromaffin cells induces rapid coating of the plasma membrane.
abstract: Stimulation of primary bovine adrenal chromaffin cells by carbachol produced a 6-fold increase in cell surface coated pits within 30 s. This coat appeared not to be recruited from a preformed pool at the plasma membrane, but from some pool transparent to electron microscopy. The number of coated pits appeared to decrease rapidly after 1 to 2 min stimulation, but processing for electron microscopy using tannic acid to enhance contrast indicated that both coated pits and closed coated vesicles were increased relative to unstimulated cells for up to 30 min. Analyses of purified adrenal medulla coated vesicles showed a lipid composition close to that expected for cell surface membrane, but there were only trace levels of plasma membrane marker enzymes. Coated vesicles contained significant amounts of both membrane and content proteins characteristic of the chromaffin granule, suggesting that medulla coated vesicles preferentially carry secretion granule proteins. The kinetics of stimulus-dependent formation of coated membrane in the cortical zone of chromaffin cells is closely similar to that observed for secretion granule membrane retrieval.
authors.forename: M J
authors.initials: MJ
authors.lastname: Geisow
authors.forename: J
authors.initials: J
authors.lastname: Childs
authors.forename: R D
authors.initials: RD
authors.lastname: Burgoyne
mesh_terms: D000313:Adrenal Medulla / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D002417:Cattle; D002458:Cell Fractionation; D002966:Clathrin / Q000378:metabolism*; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism*; D008854:Microscopy, Electron; D008970:Molecular Weight
chemical_list: D002966:Clathrin; D002217:Carbachol