Oxidative stress studied in intact mammalian cells.
Exposure of isolated rat hepatocytes to toxic doses of menadione (2-methyl-1,4-naphthoquinone) results in enhanced formation of active oxygen species, depletion of cellular glutathione and protein thiols, and perturbation of intracellular calcium ion homeostasis. An increase in cytosolic Ca2+ concentration, resulting from inhibition of the plasma membrane Ca2+ translocase by menadione metabolism, appears to be critically involved in the development of cytotoxicity.
Orrenius
S
S
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002451:Cell Compartmentation; D002470:Cell Survival / Q000187:drug effects; D003545:Cysteine / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D006706:Homeostasis; D006801:Humans; D008099:Liver / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D011809:Quinones / Q000378:metabolism / Q000633:toxicity; D013312:Stress, Physiological / Q000378:metabolism; D014812:Vitamin K / Q000633:toxicity*
D011506:Proteins; D011809:Quinones; D014812:Vitamin K; D005978:Glutathione; D003545:Cysteine; D010100:Oxygen; D002118:Calcium