Cloning and characterization of a genomic probe for malignant catarrhal fever virus.
A genomic probe specific for malignant catarrhal fever (MCF) virus was cloned by using purified viral DNA from MCF-virus strain WCll. Restriction endonuclease analysis of the purified viral DNA was used to identify the cloned viral genomic fragment. Dot blot hybridization by use of the genomic probe (pRP-5) indicated that the probe hybridized specifically with WCll-MCF virus, as well as with one other isolate of MCF-associated herpesvirus. Hybridization also was observed to a non-MCF virus strain of bovine herpesvirus.
Shih
L M
LM
Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Irving
J M
JM
Zee
Y C
YC
Pritchett
R F
RF
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D008304:Malignant Catarrh / Q000382:microbiology*; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004279:DNA, Viral; D004262:DNA Restriction Enzymes