A simple method of examining retroviral species produced by infected cells.
A simple and rapid method to examine the retroviral species present in the culture supernatant of productively infected cells is described. The method involves purification of viral genomic RNA directly from the culture supernatant and examination of that RNA by Northern or dot blot analysis. The method provides qualitative and quantitative information about the RNA species present and is particularly valuable for the detection of genetic variants in the population.
Levy
L S
LS
Tulane University School of Medicine, New Orleans, Louisiana 70112.
Lobelle-Rich
P A
PA
D015152:Blotting, Northern; D002460:Cell Line; D015151:Immunoblotting; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D012190:Retroviridae / Q000235:genetics* / Q000302:isolation & purification; D012192:Retroviridae Infections / Q000235:genetics*
D012367:RNA, Viral