[Development of test systems of immunoenzyme analysis for the detection of antibodies to the transmissible gastroenteritis virus of swine].
Two variants of enzyme immunoassay (EIA) using purified swine transmissible gastroenteritis virus antigen for sensitization of the solid phase and using infected cells on the solid phase have been developed for quantitation of specific antibodies to swine transmissible gastroenteritis (STG) virus. Both variants of the method were found to have approximately similar sensitivity and to be highly specific. The developed EIA proved to be approximately 100 times as sensitive as neutralization test. The optimal concentration of purified antigen for sensitization of microplates, as well as the multiplicity of infection of the heterologous cell culture grown in microplate wells have been determined. The above methods may be widely used in virological and epizootiological studies.
Aliper
T I
TI
Rukhadze
G G
GG
Sergeev
V A
VA
Shcheglova
E Iu
EIu
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D002478:Cells, Cultured; D003332:Coronaviridae / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005761:Gastroenteritis, Transmissible, of Swine / Q000175:diagnosis; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D009500:Neutralization Tests; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral
Razrabotka test-sistem immunofermentnogo analiza dlia vyiavleniia antitel k virusu transmissivnogo gastroénterita svineĭ.