title: Immunohistochemical identification of lysozyme and vimentin in an experimental malignant fibrous histiocytoma.
abstract: The immunoperoxidase avidin-biotin-peroxidase complex method was used to investigate the presence of histiocyte markers such as lysozyme, alpha-1-antitrypsin (A1AT) and alpha-1-anti-chymotrypsin (A1ACT) and of vimentin, a specific marker for mesenchymal differentiation, in a spontaneous and transplantable rat tumor of supposed fibroblastic-histiocytic origin. Positive staining was obtained for lysozyme and vimentin but A1AT and A1ACT were not demonstrable in any of the tumor sections. These results provide evidence for the fibro-histiocytic nature of the tumor studied and suggest its classification as a malignant fibrous histiocytoma (MFH).
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Brasil.
authors.forename: R A
authors.initials: RA
authors.lastname: Ribeiro
authors.forename: M C
authors.initials: MC
authors.lastname: Fonteles
authors.forename: S
authors.initials: S
authors.lastname: Zucoloto
authors.forename: C E
authors.initials: CE
authors.lastname: Bacchi
mesh_terms: D000818:Animals; D018219:Histiocytoma, Benign Fibrous / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D009113:Muramidase / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014746:Vimentin / Q000378:metabolism*; D000514:alpha 1-Antichymotrypsin / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000378:metabolism
chemical_list: D014746:Vimentin; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D009113:Muramidase