title: Intrathymic T cell differentiation in radiation bone marrow chimeras and its role in T cell emigration to the spleen. An immunohistochemical study.
abstract: Immunohistochemical studies were made on the regeneration of T cells of host- and donor-type in the thymus and spleen of radiation bone marrow chimeras by using B10- and B10.BR-Thy-1 congenic mice. Both the thymic cortex and the medulla were first repopulated with thymocytes of irradiated host origin, restoring the normal histologic appearance by days 11 to 14, regardless of the H-2 compatibility between the donor and the host. In Thy-1 congenic chimeras, thymocytes of donor bone marrow origin, less than 100 cells in one thymic lobe, were first recognized at day 7, when the thymus involuted to the smallest size after the irradiation. The thymocytes of donor-type then proliferated exponentially, showing a slightly faster rate when higher doses of bone marrow cells were used for reconstitution, reaching a level of 100 million by day 17 and completely replacing the cortical thymocytes of host origin by day 21. The replacement of cortical thymocytes started from the subcapsular layer in a sporadic manner. The replacement of medullary thymocytes from host- to donor-type occurred gradually between days 21 and 35, after the replacement in the cortex was completed. In the spleen, about 1 million survived cells were recovered at day 3 after the irradiation, and approximately 60% of them were shown to be host-type T cells that were observed in the white pulp areas. The host-type T cells in the spleen increased gradually after day 10, due to the influx of host-type T cells from the regenerating thymus. Thus a pronounced increase of T cells of host-type was immunohistochemically observed in the splenic white pulp between days 21 and 28, when thymocytes of host-type were present mainly in the thymic medulla. These host-type T cells were shown to persist in the spleen for a long time, as long as 420 days after the treatment. Phenotypically, they were predominantly Lyt-1+2+ when examined at day 28, but 5 mo later, they were about 50% Lyt-1+2+ and 50% Lyt-1+2-. Donor-type T cells in the spleen began to appear at about day 14 in chimeras that were transplanted with a larger dose of bone marrow cells, whereas this was slightly delayed in those grafted with a smaller dose of bone marrow cells, starting at about day 28.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.forename: K
authors.initials: K
authors.lastname: Hirokawa
authors.forename: T
authors.initials: T
authors.lastname: Sado
authors.forename: S
authors.initials: S
authors.lastname: Kubo
authors.forename: H
authors.initials: H
authors.lastname: Kamisaku
authors.forename: K
authors.initials: K
authors.lastname: Hitomi
authors.forename: M
authors.initials: M
authors.lastname: Utsuyama
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001854:Bone Marrow Cells; D002452:Cell Count; D002454:Cell Differentiation; D002465:Cell Movement; D006183:H-2 Antigens / Q000235:genetics; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera; D013154:Spleen / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology* / Q000502:physiology; D018800:Thy-1 Antigens; D013934:Thymectomy; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology / Q000502:physiology
chemical_list: D000954:Antigens, Surface; D006183:H-2 Antigens; D018800:Thy-1 Antigens