Effects of human transforming growth factors on topoisomerases from normal fibroblasts.
Normal rat kidney fibroblasts (NRK-B F49) treated at transforming doses with a gamma-like TGF, partially purified from human melanoma, showed a 3 to 5 fold increase in DNA type II topoisomerase activity. A similar effect was observed using EGF and a partially purified alfa TGF from rabbit fetuses. DNA type I topoisomerase activity, from the same cells, was not significantly modified by treatment with these growth factors. Topoisomerase II stimulation was dependent on mRNA synthesis. The possible role of topoisomerase II in phenotypical cell transformation induced by TGF is discussed.
Genovese
J A
JA
Instituto de Biologia Celular, Facultad de Medicina, UBA.
Crespi
M D
MD
Castro
G
G
Medina
F H
FH
Baldi
A
A
D000818:Animals; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005333:Fetus; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008545:Melanoma / Q000473:pathology; D011817:Rabbits; D051381:Rats; D015291:Transforming Growth Factors / Q000302:isolation & purification / Q000494:pharmacology*
D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II