A 22 kDa ras-related G-protein is the substrate for an ADP-ribosyltransferase from Clostridium botulinum.
A ribosyltransferase from C. botulinum type D ADP-ribosylated a protein of 22 kDa (p22) in human astrocytoma (1321N1) cells. ADP-ribosylation of membrane-bound p22 was potentiated by 2 mM MgCl2 or guanine nucleotides but was much reduced in the presence of 10 mM Mg2+ plus GTP gamma S. p22 was immunoprecipitated by a monoclonal antibody (142-24E05) raised against a peptide sequence common to the ras gene family but not by other ras or G-protein antibodies. p22 was also ADP-ribosylated in Drosophila but was not detected in Dictyostelium. These data suggest that the 22 kDa botulinum toxin substrate is a GTP-binding protein and a member of the ras protein family.
Quilliam
L A
LA
Department of Pharmacology, University of California San Diego, La Jolla 92093.
Brown
J H
JH
Buss
J E
JE
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D001254:Astrocytoma; D002460:Cell Line; D003014:Clostridium botulinum / Q000201:enzymology*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007700:Kinetics; D009713:Nucleotidyltransferases / Q000378:metabolism*; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras); D013379:Substrate Specificity
D011518:Proto-Oncogene Proteins; D000246:Adenosine Diphosphate Ribose; D009713:Nucleotidyltransferases; D011062:Polynucleotide Adenylyltransferase; D019204:GTP-Binding Proteins; D016283:Proto-Oncogene Proteins p21(ras)