Conversion of leumorphin (dynorphin B-29) to dynorphin B and dynorphin B-14 by thiol protease activity.
Dynorphin B (rimorphin) is formed from leumorphin (dynorphin B-29) by the action of a thiol protease from rat brain membranes, in a single step. This represents a "single-arginine cleavage" between threonine-13 and arginine-14 of the substrate. We have observed that in addition to dynorphin B, dynorphin B-14 is formed from dynorphin B-29. Among the various protease inhibitors tested, none except p-chloromercuribenzensulfonic acid inhibited the formation of the two products. Both temperature and pH had similar effects on the formation of dynorphin B-14 and dynorphin B. The inhibitory potencies of adrenocorticotropic hormone, peptide E, and dynorphin A were virtually identical for the formation of the two products. These results suggest that the same enzyme may be responsible for the formation of dynorphin B-14 and dynorphin B.
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Goldstein
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D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D003546:Cysteine Endopeptidases; D004399:Dynorphins / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D004723:Endorphins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004745:Enkephalins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D011498:Protein Precursors / Q000378:metabolism*; D013696:Temperature
D004723:Endorphins; D004745:Enkephalins; D010455:Peptides; D011480:Protease Inhibitors; D011498:Protein Precursors; D004399:Dynorphins; C039933:peptide E (adrenal medulla); C036200:rimorphin; D000324:Adrenocorticotropic Hormone; C033660:preproenkephalin; D010450:Endopeptidases; D003546:Cysteine Endopeptidases