Heterologous up-regulation of the 1,25-dihydroxyvitamin D3 receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells.
1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) receptor content in cultured osteogenic sarcoma cells (UMR-106) was found to be increased after treatment with both bovine and human PTH and human PTH-like peptide (hPLP). The dose dependent increase of receptors was preceded by a dose dependent stimulation of cAMP production. This suggests a role for cAMP as mediator of the PTH- and hPLP-induced 1,25-(OH)2D3 receptor up-regulation. Furthermore, evidence was obtained that new mRNA and de novo receptor synthesis is involved in this heterologous 1,25-(OH)2D3 receptor up-regulation.
Pols
H A
HA
Department of Internal Medicine III, Erasmus University Medical School, Rotterdam, The Netherlands.
van Leeuwen
J P
JP
Schilte
J P
JP
Visser
T J
TJ
Birkenhäger
J C
JC
D000818:Animals; D002117:Calcitriol / Q000378:metabolism*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D007700:Kinetics; D010006:Osteoblasts / Q000378:metabolism; D012516:Osteosarcoma; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism*; D019379:Teriparatide
D010281:Parathyroid Hormone; D010446:Peptide Fragments; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D019379:Teriparatide; D003609:Dactinomycin; D003513:Cycloheximide; D000242:Cyclic AMP; D002117:Calcitriol