Identification of a cyclic adenosine monophosphate-responsive element in the rat corticotropin-releasing hormone gene.
The molecular mechanisms involved in the regulation of expression of the rat CRH gene have been examined in rat pheochromocytoma (PC-12) cells transiently transfected with a chimeric gene containing 1.4 kilobases of rat CRH 5'-flanking DNA fused to the bacterial reporter gene encoding chloramphenicol acetyltransferase. Cyclic AMP analogs and activators of adenylate cyclase positively regulate the expression of this chimeric gene in PC-12 cells, inducing chloramphenicol acetyltransferase activity more than 15-fold. The DNA sequence required for this response to cAMP has been localized to a 59 base pair region located between 238 and 180 base pairs 5' to the putative CRH mRNA cap site. This sequence can confer cAMP-responsiveness on a heterologous promoter in an orientation independent fashion and has homology to cAMP regulatory regions from a number of other eukaryotic genes.
Seasholtz
A F
AF
Vollum Institute for Advanced Biomedical Research Oregon Health Sciences University, Portland 97201.
Thompson
R C
RC
Douglass
J O
JO
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D000242:Cyclic AMP / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010673:Pheochromocytoma / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000502:physiology
D004247:DNA; D003346:Corticotropin-Releasing Hormone; D000242:Cyclic AMP