title: Amino acid and nucleotide sequence homologies among E. coli RNA polymerase core enzyme subunits, DNA primase, elongation factor Tu, F1-ATPase alpha, ribosomal protein L3, DNA polymerase I, T7 phage DNA polymerase, and MS2 phage RNA replicase beta subunit.
abstract: The 330 residue-long N-terminal domains (NTDs) of beta and beta' subunits of the Escherichia coli RNA polymerase (RPase) core enzyme were found to be significantly homologous to the entire length of its alpha subunit. The C-terminal domains (CTDs) of the RPase beta subunit and DNA primase (dnaG protein) were not only strongly homologous to each other but also considerably homologous to the RPase alpha, suggesting that an alpha subunit-like enzyme must have been commonly ancestral to core enzyme subunits and primase. The N-terminal region (NTR) of RPase alpha was also found to show significant homologies with NTRs of the E. coli EF-Tu and F1-ATPase alpha subunit, and a possible weak homology with ribosomal protein L3. A most important finding was that the C-terminal regions (CTRs) of DNA polymerase (DPase) I, T7 phage DPase and MS2 phage RNA replicase beta subunit are closely homologous with one another. These CTRs showed considerable homologies to RPase alpha NTD and RPase beta CTD. These conclusions are based on statistical evaluations of homologies in base and/or amino acid sequence alignments.
authors.forename: K
authors.initials: K
authors.lastname: Ohnishi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003090:Coliphages / Q000201:enzymology; D004256:DNA Polymerase I / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology*; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013604:T-Phages / Q000201:enzymology
chemical_list: D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase; D010444:Peptide Elongation Factor Tu; D006180:Proton-Translocating ATPases