title: Increased detection rate for varicella-zoster virus with combination of two techniques.
abstract: Standard cell culture and centrifugation culture were compared for the isolation of varicella-zoster virus from 337 clinical specimens. Of a total of 85 (25%) positive specimens, 67 (78.8%) were positive by centrifugation culture alone and 18 (21.2%) were positive by both methods. When epithelial cells from the specimen were included in the vial inoculum, these cells stained positive and increased the varicella-zoster virus detection rate by 30%.
authors.affiliation: SmithKline Bio-science Laboratories, Norristown, Pennsylvania 19403.
authors.forename: P G
authors.initials: PG
authors.lastname: West
authors.forename: B
authors.initials: B
authors.lastname: Aldrich
authors.forename: R
authors.initials: R
authors.lastname: Hartwig
authors.forename: G J
authors.initials: GJ
authors.lastname: Haller
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells; D014645:Herpesvirus 3, Human / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D012867:Skin / Q000382:microbiology*; D014776:Virus Cultivation / Q000379:methods