Synthesis and assembly of a functionally active recombinant platelet-derived growth factor AB heterodimer.
A Chinese hamster ovary cell line that stably expresses transfected human platelet-derived growth factor (PDGF) A and B chain precursors was established. All three dimeric combinations of PDGF chains were produced by this cell line; their biosynthesis, assembly, and processing were followed by pulse-chase analyses. PDGF-AA, PDGF-AB, and PDGF-BB were processed to Mr values of about 30,000 and were accumulated in these forms in the medium. In addition, PDGF-BB was further processed to a 24-kDa component, which remained cell-associated. The major secreted component was PDGF-AB, which was purified and shown to have structural and functional characteristics indistinguishable from PDGF-AB purified from human platelets.
Ostman
A
A
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Rall
L
L
Hammacher
A
A
Wormstead
M A
MA
Coit
D
D
Valenzuela
P
P
Betsholtz
C
C
Westermark
B
B
Heldin
C H
CH
D000818:Animals; D002460:Cell Line; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010957:Plasmids; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014162:Transfection
D046911:Macromolecular Substances; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D017479:Receptors, Platelet-Derived Growth Factor