beta-Adrenergic agonist activity of a monoclonal anti-idiotypic antibody.
Hybridoma cells bearing monoclonal antibody against the beta-adrenergic ligand alprenolol were used as an immunogen to raise monoclonal anti-idiotypic antibodies. Of six anti-idiotypic antibodies, which inhibit ligand binding, three were able to recognize beta-adrenergic receptors. One of them, mAb2B4, an IgM that could be amplified into ascites, binds to the beta-adrenergic catecholamine receptors of intact epidermoid A431 cells and precipitates receptors solubilized from plasma membranes by digitonin. This antibody identifies the beta 2-adrenergic receptor of A431 cells as a single 55-kDa protein and stimulates adenylate cyclase activity. This stimulation is inhibited by the beta-adrenergic antagonist propranolol.
Guillet
J G
JG
Kaveri
S V
SV
Durieu
O
O
Delavier
C
C
Hoebeke
J
J
Strosberg
A D
AD
D000262:Adenylyl Cyclases / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000276:immunology*; D000526:Alprenolol / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002478:Cells, Cultured; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D011943:Receptors, Adrenergic, beta / Q000276:immunology
D000318:Adrenergic beta-Agonists; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D011943:Receptors, Adrenergic, beta; D000526:Alprenolol; D000262:Adenylyl Cyclases