title: Top concentrations of dynorphin-like immunoreactivity in fractions of rat anterior pituitary cells enriched in gonadotrophs.
abstract: A possible relationship between anterior pituitary cells containing luteinizing hormone (LH) and those containing the endogenous opioid dynorphin and other proenkephalin B-derived peptides was examined. Gonadotroph-enriched and gonadotroph-depleted cell fractions were prepared from cell suspensions of adult female rat anterior pituitary glands by centrifugal elutriation. Fractions with high or low concentrations of LH contained also high or low concentrations of dynorphin-like immunoreactivity. A positive correlation was found between the content in the eluted cell fractions of LH and dynorphin-like immunoreactivity with a correlation coefficient and a slope of the regression line close to one. Therefore, LH-containing and dynorphin-containing cells of the rat adenohypophysis exhibit almost the same characteristics under the conditions of centrifugal elutriation. This is consistent with the suggestion that dynorphin and other proenkephalin B-derived peptides may be colocalized with LH and/or follicle-stimulating hormone in at least some of the gonadotrophs of the rat anterior pituitary gland.
authors.forename: W
authors.initials: W
authors.lastname: Knepel
authors.forename: M
authors.initials: M
authors.lastname: Schwaninger
authors.forename: C
authors.initials: C
authors.lastname: Helm
authors.forename: L
authors.initials: L
authors.lastname: Kiesel
mesh_terms: D000818:Animals; D002498:Centrifugation; D004399:Dynorphins / Q000032:analysis*; D005260:Female; D007986:Luteinizing Hormone / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010446:Peptide Fragments; C026843:dynorphin (1-13); D004399:Dynorphins; D007986:Luteinizing Hormone