High-performance immobilized-metal-ion affinity chromatography of peptides and proteins.
The present status of the undeveloped branch of high-performance immobilized-metal-ion affinity chromatography (HPIMAC) is reviewed. As demonstrated, high resolution of peptides and protein mixtures can be obtained. Under specified conditions, more than 1000 runs can be made repeatedly on a single column without much change in pattern or recovery. Occasionally, 10(3)- to 10(4)-fold purification of a protein can be obtained in a single chromatographic run.
Porath
J
J
Institute of Biochemistry, University of Uppsala, Sweden.
D002846:Chromatography, Affinity / Q000295:instrumentation*; D003432:Cross-Linking Reagents; D008084:Lipoxygenase / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D011108:Polymers; D011506:Proteins / Q000302:isolation & purification*; D012685:Sepharose; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D003432:Cross-Linking Reagents; D010455:Peptides; D011108:Polymers; D011506:Proteins; D012822:Silicon Dioxide; D012685:Sepharose; D008084:Lipoxygenase