[Radioreceptor method of determination of opioid peptides in the rat brain].
A procedure is described for quantitative radioreceptor estimation of opioid peptides in rat brain using lyophilized membrane preparations. As opiate receptors exhibit high rate of heterogeneity estimation of delta- and mu-opioid activities was carried out by means of displacing of 3H-DADLE and 3H-DAGO, respectively. Content of opioid peptides in brain of preadolescent rats was studied during experimental burn trauma using the procedure developed. An increase in the delta- and mu-opioid activities was found in rat brain tissue within one day after thermal burns.
Davletov
E G
EG
Kurochkin
I N
IN
Zaĭtsev
S V
SV
D000818:Animals; D001923:Brain Chemistry; D002056:Burns / Q000378:metabolism; D004723:Endorphins / Q000032:analysis*; D008297:Male; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000032:analysis*
D004723:Endorphins; D011957:Receptors, Opioid
Radioretseptornyĭ metod opredeleniia opioidnykh peptidov v golovnom mozge krys.