title: Limited tryptic proteolysis of the benzodiazepine binding proteins in different species reveals structural homologies.
abstract: Peptide mapping can be used to elucidate further the structural similarities of the benzodiazepine binding proteins in different vertebrate species. Crude synaptic membrane preparations were photoaffinity-labeled with [3H]flunitrazepam and subsequently degraded with various concentrations of trypsin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by fluorography allowed a comparison of the molecular weights of photolabeled peptides in different species. Tryptic degradation led to a common peptide of 40K in all species investigated, a finding indicating that the benzodiazepine binding proteins are structurally homologous in higher bony fishes and tetrapods.
authors.affiliation: Institut für Humangenetik, Universität Bonn, F.R.G.
authors.forename: W
authors.initials: W
authors.lastname: Friedl
authors.forename: K U
authors.initials: KU
authors.lastname: Lentes
authors.forename: E
authors.initials: E
authors.lastname: Schmitz
authors.forename: P
authors.initials: P
authors.lastname: Propping
authors.forename: J
authors.initials: J
authors.lastname: Hebebrand
mesh_terms: D000345:Affinity Labels; D000489:Alligators and Crocodiles; D000818:Animals; D002417:Cattle; D004372:Ducks; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005445:Flunitrazepam / Q000378:metabolism; D006168:Guinea Pigs; D007076:Iguanas; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism*; D010777:Photochemistry; D011898:Ranidae; D011963:Receptors, GABA-A / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D012911:Snakes; D013045:Species Specificity; D013552:Swine; D013570:Synaptic Membranes / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*
chemical_list: D000345:Affinity Labels; D010446:Peptide Fragments; D011963:Receptors, GABA-A; D005445:Flunitrazepam; D014357:Trypsin