Cyclic AMP induces metallothionein gene expression in rat hepatocytes but not in rat kidney.
Cyclic AMP analogues induced expression of metallothionein-I (MT-I) mRNA in adult rat liver and in rat hepatocytes cultured in serum-free medium. This induction occurred via an increased rate of transcription of the MT-I gene. The effect of cyclic AMP analogues was tissue-specific since no induction of MT-I mRNA occurred in rat kidney. Induction of MT-I mRNA by a combination of cyclic AMP analogue and dexamethasone was additive in liver and cultured hepatocytes, indicating that induction occurred via independent regulatory pathways.
Nebes
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Department of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, PA 15261.
DeFranco
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Morris
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D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008668:Metallothionein / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013873:Thionucleotides / Q000494:pharmacology
D012333:RNA, Messenger; D013873:Thionucleotides; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D003994:Bucladesine; D003907:Dexamethasone; D008668:Metallothionein; D000242:Cyclic AMP