title: Modulation of Escherichia coli N-acetylmuramoyl-L-alanine amidase activity by phosphatidylglycerol.
abstract: The activity of pure Escherichia coli murein (peptidoglycan) amidase (N-acetylmuramoyl-L-alanine amidase, EC 3.5.1.28) was measured after preincubation with E. coli phosphatidylglycerol microdispersions in final concentration ranging over micro- and millimolarities. The enzyme activity was increased up to 160% of the control for phosphatidylglycerol concentrations increasing from 2 to 50 microM. After a plateau extending from 0.05 to 0.3 mM, higher phosphatidylglycerol concentrations inactivated the enzyme down to 15% of initial activity for concentrations of 2 mM. Positive kinetic cooperativity was observed for the activation as well as for the inactivation processes. Cardiolipin (or diphosphatidylglycerol) from the same origin and under same conditions had no significant effect. Molecular sieving experiments have shown that, when inactivated, the enzyme remained firmly bound to the phosphatidylglycerol vesicles, whereas the activated phosphatidylglycerol-enzyme complex was totally dissociable by dilution. Activated phosphatidylglycerol complexes were recovered by gel exclusion chromatography at equilibrium in 40 microM phosphatidylglycerol. Possible physiological meaning of the results is briefly discussed in the context of our work and that done previously by others.
authors.forename: E
authors.initials: E
authors.lastname: Vanderwinkel
authors.forename: M
authors.initials: M
authors.lastname: De Vlieghere
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D002308:Cardiolipins / Q000494:pharmacology; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D010715:Phosphatidylglycerols / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D002308:Cardiolipins; D010715:Phosphatidylglycerols; D000581:Amidohydrolases; D009238:N-Acetylmuramoyl-L-alanine Amidase