Fusicoccin stimulates the H+-ATPase of plasmalemma in isolated membrane vesicles from radish.
The effect of fusicoccin on the plasmalemma H+-ATPase has been investigated in a membrane fraction from 24 h old radish seedlings, in which Mg:ATP-dependent H+-transport is mediated only by the plasmalemma H+-ATPase. Fusicoccin stimulated the plasmalemma H+-ATPase - i.e. Mg:ATP-dependent intravesicular acidification, hyperpolaryzation of delta psi and ATPase activity -, when these activities were measured at the physiologically relevant pHs of 7.3 to 7.6. No effect of FC on the plasmalemma H+-ATPase was evident at pH 6.6.
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D006027:Glycosides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008861:Microsomes / Q000201:enzymology; D010766:Phosphorylation; D010944:Plants / Q000201:enzymology*; D006180:Proton-Translocating ATPases / Q000032:analysis*
D006027:Glycosides; C007808:fusicoccin; D006180:Proton-Translocating ATPases