Effects of phenothiazines on cultured retinal pigment epithelial cells.
Phenothiazines (chlorpromazine, trifluoperazine, prochlorperazine, and fluphenazine) showed dose-dependent inhibition of phagocytosis of latex particles by cultured chick retinal pigment epithelial cells at concentrations of 10(-5)-10(-14) mol/l. Calmodulin antagonists (W-5, W-7, W-12, and W-13) showed similar effects as phenothiazines at concentrations of 10(-5)-10(-14) mol/l. These reactions were partially reversible at a concentration of 10(-9) mol/l. Cells cultured for 3 days in the presence of 10(-5) mol/l chlorpromazine or 10(-5) mol/l W-7 demonstrated morphologic alterations in their microvilli which were similar to those seen after cytochalasin B treatment, i.e., thinning and elongation of the microvilli with honeycomb-like changes.
Matsumura
M
M
Yamakawa
R
R
Shirakawa
H
H
Ogino
N
N
D000818:Animals; D014150:Antipsychotic Agents / Q000633:toxicity*; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002478:Cells, Cultured; D002642:Chick Embryo; D008854:Microscopy, Electron; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D010587:Phagocytosis / Q000187:drug effects; D010640:Phenothiazines; D010857:Pigment Epithelium of Eye / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure
D014150:Antipsychotic Agents; D002147:Calmodulin; D010640:Phenothiazines