Healing of cutaneous and mucosal wounds grafted with collagen-glycosaminoglycan/silastic bilayer membranes: a preliminary report.
This report describes the healing of cutaneous wounds in experimental animals grafted with collagen-glycosaminoglycan (GAG) matrix/Silastic (Dow Corning Corp., Midland, MI) bilayers; assesses the feasibility of using collagen-GAG matrix as a vehicle for delivering culture-selected, autogenous fibroblasts to cutaneous wound sites; and evaluates the use of collagen-GAG/Silastic bilayers as mucosal substitutes. Cutaneous and mucosal wounds in New Zealand white rabbits were grafted with either acellular collagen-GAG/Silastic membrane or collagen-GAG/Silastic membrane previously seeded with cultured autogenous fibroblasts. Over 63 days, wound sites were analyzed at intervals based on wound contraction and histology. Cutaneous wound successfully incorporated grafted collagen-GAG matrix and were significantly inhibited in their rate and extent of wound contraction. Seeding membrane matrices with autogenous, cultured fibroblasts before grafting caused a marked increase in cellularity that persisted throughout the postgraft period. In mucosa, matrices were exteriorized rather than incorporated. This work suggests that collagen-GAG/Silastic bilayer may have value as a dermal substitute and, more significantly, may be appropriate as a vehicle for delivering culture-selected fibroblasts to cutaneous wound sites.
Bertolami
C N
CN
Shriners Burn Institute, Boston, MA.
Ellis
D G
DG
Donoff
R B
RB
D000818:Animals; D003094:Collagen; D062109:Dermatologic Surgical Procedures; D005347:Fibroblasts / Q000502:physiology; D006025:Glycosaminoglycans; D008567:Membranes, Artificial; D009061:Mouth Mucosa / Q000503:physiopathology* / Q000601:surgery; D011817:Rabbits; D012826:Silicone Elastomers; D012828:Silicones; D012867:Skin / Q000503:physiopathology*; D014945:Wound Healing
D006025:Glycosaminoglycans; D008567:Membranes, Artificial; D012826:Silicone Elastomers; D012828:Silicones; D003094:Collagen