Characterization of two molecular weight classes of cadmium binding proteins from the mussel, Mytilus edulis (L.).
Inducible cadmium binding proteins (Cd-BP) in the mussel, Mytilus edulis, were resolved into two molecular weight components by gel permeation chromatography on Sephadex G-75. Each of these two molecular weight components was further resolved into four subcomponents by DEAE ion exchange chromatography. All eight subcomponents bound cadmium and exhibited significant u.v. absorption at 254 and little absorption at 280 nm. Based on amino acid composition analysis two classes of proteins were identified, one having higher cysteine (approximately 25 mole %) and lower serine and glutamic acid contents compared to the other class.
Frazier
J M
JM
George
S S
SS
Overnell
J
J
Coombs
T L
TL
Kagi
J
J
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D049872:Bivalvia / Q000032:analysis / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000032:analysis; D008668:Metallothionein / Q000302:isolation & purification*; D008970:Molecular Weight; D013329:Structure-Activity Relationship
D000596:Amino Acids; C004203:cadmium-binding protein; D002104:Cadmium; D008668:Metallothionein