Some factors affecting [3H]tyrosine binding to plasma membrane preparation of rat corpora lutea in vitro.
Temperature, pH, enzymes and ions affected the two types of [3H]tyrosine binding sites differently. The optimal temperature and pH for the high affinity sites were 24 C and 7.4, and for the low affinity sites 37 degrees C and 7.0, respectively. The [3H]tyrosine binding to the high and low affinity sites was not observed at 55 degrees C, nor at pH 6.0 or pH 8.5. Trypsin reduced [3H]tyrosine binding to the high affinity sites, but had no effect on the binding to the low affinity sites. Neuraminidase reduced [3H]tyrosine binding to the low affinity sites, but had no effect on the binding to the high affinity sites. None of cycloheximide, kCG, cAMP, and progesterone affected [3H]tyrosine binding.
Gao
E W
EW
Department of Physiology, Harbin Medical University.
Cheng
Z P
ZP
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D009439:Neuraminidase / Q000494:pharmacology; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects*; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*
D011956:Receptors, Cell Surface; D015636:Magnesium Chloride; D014443:Tyrosine; D009439:Neuraminidase; D014357:Trypsin; D008274:Magnesium