title: Evidence of gene amplification in tunicamycin-resistant Chinese hamster ovary cells.
abstract: Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast transferase gene, ALG7. The data presented suggest moderate amplification of the N-acetylglucosaminyl-1-phosphate transferase gene occurred in these cells, consistent with the previously observed chromosomal translocations and increased enzymatic activity. This is the first example of gene amplification as a mechanism for aberrations in N-linked glycosylation.
authors.affiliation: Department of Biochemistry, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland.
authors.forename: J R
authors.initials: JR
authors.lastname: Scocca
authors.forename: K O
authors.initials: KO
authors.lastname: Hartog
authors.forename: S S
authors.initials: SS
authors.lastname: Krag
mesh_terms: D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D015342:DNA Probes; D004351:Drug Resistance; D005260:Female; D005784:Gene Amplification; D009693:Nucleic Acid Hybridization; D010053:Ovary; D010770:Phosphotransferases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017855:Transferases (Other Substituted Phosphate Groups); D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D015342:DNA Probes; D014415:Tunicamycin; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C023222:UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase