title: [Phosphoprotein phosphatase nonspecifically hydrolyzes CoA].
abstract: CoA hydrolysis was studied by a homogenous phosphoprotein phosphatase (EC 3.1 3.16) preparation from bovine spleen nuclei at pH 5.8. Phosphoprotein phosphatase catalyzed hydrolysis of the CoA 3'-phosphoester bond to form dephospho-CoA and Pi. The Km value for phosphoprotein phosphatase with CoA as substrate was 3.7 mM, the specific activity - 0.26 mmol Pi.min-1.mg-1. Phosphoprotein phosphatase did not essentially catalyze the calcium pantothenate hydrolysis (not more than 2% as compared with the CoA hydrolysis rate).
authors.forename: V I
authors.initials: VI
authors.lastname: Reziapkin
authors.forename: A G
authors.initials: AG
authors.lastname: Moiseenok
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003065:Coenzyme A / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology
chemical_list: D010749:Phosphoprotein Phosphatases; D003065:Coenzyme A
vernacular_title: Fosfoproteinfosfataza nespetsificheski gidrolizuet CoA.