title: The effect of sodium pump blockade and denervation on the steady-state sodium permeability of mouse skeletal muscle fibres.
abstract: The Na+ pump inhibitor ouabain produced a 25 mV depolarization of mouse extensor digitorum longus muscle fibres in 2 h. Approximately 40% of this depolarization could be prevented by the inclusion of tetrodotoxin (TTX) in the incubation fluid, and the membrane potential of ouabain-depolarized fibres increased when external Na+ ion concentration was reduced. TTX and Na+ reduction were without effect on fibres incubated for 2 h in the control saline or on fibres depolarized by raising external K+ ion concentration. Ouabain induced a fall in input resistance in fibres of reduced chloride conductance. Part of the depolarization induced in the majority of fibres by K+-free solutions was also TTX sensitive. These results suggest that the steady-state Na+ permeability of muscle fibres is increased after Na+ pump blockade. The ouabain-induced depolarization of skeletal muscle therefore cannot be taken as evidence for a significant contribution of an electrogenic Na+ pump to the resting membrane potential. TTX also reverses much of the depolarization which occurs after denervation of skeletal muscle. Denervation and ouabain both increase the steady-state Na+ permeability of the sarcolemma, possibly by an action on the same population of Na+ channels.
authors.affiliation: Department of Physiology, University of Dundee.
authors.forename: S R
authors.initials: SR
authors.lastname: Seabrooke
authors.forename: M R
authors.initials: MR
authors.lastname: Ward
authors.forename: N K
authors.initials: NK
authors.lastname: White
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009121:Muscle Denervation; D009132:Muscles / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
chemical_list: D013779:Tetrodotoxin; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase