Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands.
A simple and sensitive radioimmunoassay was developed for measurement of immunoreactive atrial natriuretic factor (IR-ANF) in rat and human plasma and in rat atria. The two atria contain about 20 micrograms ANF per rat. The right atrium contained 2.5 times more ANF than did the left. Ether anesthesia and morphine markedly increased IR-ANF in rat plasma. The concentration of IR-ANF in plasma of clinically normal human subjects was 65.3 +/- 2.5 pg/ml. Paroxysmal tachycardia and rapid atrial pacing significantly increased IR-ANF in human plasma. Two- to seven-fold higher concentrations were found in coronary sinus blood than in the peripheral circulation. In the plasma of rats and humans, circulating ANF is probably a small-molecular-weight peptide. ANF acts on the adrenal and the pituitary. ANF inhibits aldosterone secretion from rat zona glomerulosa and steroid secretion by bovine adrenal zona glomerulosa and fasciculata. ANF stimulated the basal secretion of arginine vasopressin (AVP) in vitro and inhibited KCl-stimulated release of AVP.
Gutkowska
J
J
Horký
K
K
Schiffrin
E L
EL
Thibault
G
G
Garcia
R
R
De Léan
G A
GA
Hamet
P
P
Tremblay
J
J
Anand-Srivastava
M B
MB
Januszewicz
P
P
D000067956:Adenylyl Cyclase Inhibitors; D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000450:Aldosterone / Q000378:metabolism; D000595:Amino Acid Sequence; D000777:Anesthetics / Q000494:pharmacology; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000097:blood / Q000494:pharmacology; D001681:Biological Assay; D001800:Blood Specimen Collection; D004231:Diuresis / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006162:Guanylate Cyclase / Q000378:metabolism; D006325:Heart Atria / Q000032:analysis; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D008297:Male; D009318:Natriuresis / Q000187:drug effects; D010446:Peptide Fragments / Q000032:analysis; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D012016:Reference Values
D000067956:Adenylyl Cyclase Inhibitors; D000777:Anesthetics; D010446:Peptide Fragments; D001127:Arginine Vasopressin; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D000324:Adrenocorticotropic Hormone; D006162:Guanylate Cyclase; D006854:Hydrocortisone