title: Proline-hyperproducing strains of Serratia marcescens: enhancement of proline analog-mediated growth inhibition by increasing osmotic stress.
abstract: Proline-producing strains of Serratia marcescens were more osmotolerant than wild-type strains. Growth inhibition by proline analogs was significantly enhanced by increasing the osmotic stress of the medium. Mutants resistant to azetidine-2-carboxylate were derived from a proline-producing strain, SP126, under a high osmotic condition. One of the mutants, strain SP187, produced 56 mg of L-proline per ml of medium containing sucrose and urea. This amount was ca. 3 times larger than that produced by strain SP126. The intracellular glutamate content which decreased in strain SP126 was restored in strain SP187. The glutamate dehydrogenase level of strain SP187 was 5 times higher than that of strain SP126.
authors.forename: M
authors.initials: M
authors.lastname: Sugiura
authors.forename: M
authors.initials: M
authors.lastname: Kisumi
mesh_terms: D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D011392:Proline / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000502:physiology; D012706:Serratia marcescens / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D014882:Water-Electrolyte Balance
chemical_list: D005971:Glutamates; D018698:Glutamic Acid; D011392:Proline; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase