Interaction of herpes simplex virus type 2 with a rat glioma cell line.
The interaction between herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) and two neural cell lines, mouse neuroblastoma (N1E-115) and rat glioma (C6-BU-1), was investigated. N1E-115 cells were permissive to both types of HSV. In C6-BU-1 cells, on the other hand, all the HSV-1 strains tested so far showed persistent infection, and the infectious virus of HSV-2 strains disappeared spontaneously. The HSV-2-infected C6-BU-1 cells were positive for HSV-2-specific DNA sequences, virus-specific RNA, HSV-2-specific antigens and thymidine kinase activity, when no infectious virus was detected. The HSV-2 was reactivated from those C6-BU-1 cells by superinfection with murine cytomegalovirus (MCMV), but not with UV-irradiated MCMV or human cytomegalovirus. The reactivated HSV-2 was identical to the parental virus, when examined by restriction endonuclease cleavage analysis.
Sakihama
K
K
Department of Microbiology, Miyazaki Medical College.
Eizuru
Y
Y
Minamishima
Y
Y
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D005910:Glioma / Q000382:microbiology; D009447:Neuroblastoma / Q000382:microbiology; D012367:RNA, Viral / Q000302:isolation & purification; D051381:Rats; D018139:Simplexvirus / Q000201:enzymology / Q000302:isolation & purification / Q000502:physiology*; D013937:Thymidine Kinase / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000382:microbiology*; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral; D013937:Thymidine Kinase