Bordetella pertussis filamentous hemagglutinin gene: molecular cloning of a potential coding sequence.
We have constructed an expression library of Bordetella pertussis DNA sequences, cloned in Escherichia coli. Random DNA fragments were inserted into the beta-galactosidase gene of bacteriophage lambda gt11 and plaques were screened with antibodies directed against filamentous hemagglutinin. The antigen-positive clone obtained expressed B. pertussis sequences as polypeptides fused to beta-galactosidase. The fusion polypeptides reacted both with antibodies to beta-galactosidase and with antibodies to filamentous hemagglutinin. The positive clone contains B. pertussis DNA sequences as determined by specific hybridization to Bordetella DNA.
Reiser
J
J
Friedman
R L
RL
Germanier
R
R
D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006388:Hemagglutinins / Q000235:genetics*; D009693:Nucleic Acid Hybridization
D004269:DNA, Bacterial; D006388:Hemagglutinins