[Group separation of caseins from cow milk on hydroxyapatite].
Ion exchange-chromatography method with the use of hydroxy apatite is considered one of the methods used for the casein fractionation particularly those containing phosphate in its composition. Simplicity of hydroxyapatite-preparation and its easily use in casein fractionation in batches are advantages of this method. Sephadex G-25 or Agarose is used to easement the sample passage through the column. By using of hydroxy-apatite in batches, alpha s-casein can easily separated with the use of buffer solution 0.006 M phosphate at pH 6.6 and the elution is carried out by buffer solution containing 0.25 M KH2PO4, 0.2 M KCl, 4.5 M urea and 0.002 M di-thiotreitol. Before the elution of alpha s-casein, beta- and kappa-casein are separated using buffer solutions containing 0.005 M and 0.1 M KH2PO4 in addition to the previously mentioned components.
Sienkiewicz
T
T
Humboldt-Universität zu Berlin, Sektion Nahrungsgüterwirtschaft und Lebensmitteltechnologie, DDR.
Mohamed
S H
SH
Lipke
B
B
D000818:Animals; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D017886:Durapatite; D004586:Electrophoresis; D006882:Hydroxyapatites; D008892:Milk / Q000032:analysis*
D002364:Caseins; D006882:Hydroxyapatites; D017886:Durapatite
Gruppentrennung der Caseine aus Kuhmilch an Hydroxyapatit.