title: First trimester diagnosis of hypophosphatasia with a monoclonal antibody to the liver/bone/kidney isoenzyme of alkaline phosphatase.
abstract: Prenatal diagnosis of hypophosphatasia was made by alkaline phosphatase (ALP) assay on a chorionic villus sample taken in the first trimester. Monoclonal antibodies against the liver/bone/kidney (LBK) and placental isoenzymes of ALP were used, and the bound isoenzymes were quantified by an amplification system. Very low activities of the LBK isoenzyme indicated an affected fetus. Diagnosis was confirmed by ultrasound scan at 15 weeks' gestation, and by ALP measurement in amniotic fluid supernatant and fetal serum.
authors.forename: R C
authors.initials: RC
authors.lastname: Warren
authors.forename: C F
authors.initials: CF
authors.lastname: McKenzie
authors.forename: C H
authors.initials: CH
authors.lastname: Rodeck
authors.forename: G
authors.initials: G
authors.lastname: Moscoso
authors.forename: D J
authors.initials: DJ
authors.lastname: Brock
authors.forename: L
authors.initials: L
authors.lastname: Barron
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis / Q000276:immunology*; D000653:Amniotic Fluid / Q000201:enzymology; D000911:Antibodies, Monoclonal; D001842:Bone and Bones / Q000201:enzymology; D002824:Chorionic Villi / Q000201:enzymology; D005260:Female; D006801:Humans; D007014:Hypophosphatasia / Q000175:diagnosis* / Q000473:pathology; D007527:Isoenzymes / Q000032:analysis / Q000276:immunology*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011296:Prenatal Diagnosis; D014463:Ultrasonography
chemical_list: D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D000469:Alkaline Phosphatase