V79 Chinese-hamster cells rendered resistant to high cadmium concentration also become resistant to oxidative stress.
Chinese hamster cells (V79) resistant to high concentrations of Cd2+ in the medium were obtained by using the procedure of Beach & Palmiter [(1981) Proc. Natl. Acad. Sci. U.S.A. 78, 2110-2114], which in mouse led to amplification of metallothionein (MT) genes and to an enrichment in cellular MT. The Cd-resistant V79 clones isolated were significantly more resistant than parental cells to oxidative stress by extracellular H2O2 or a mixture of H2O2 and superoxide anion (O2-) generated by xanthine oxidase plus acetaldehyde. On a per-cell basis, there was no difference between the two cells in their total H2O2-decomposing or O2-(-)dismutating activity. The most likely explanation is that an enrichment in MT content in the Cd-resistant cells was responsible for this effect, because of the antioxidant properties already described for this protein.
Mello-Filho
A C
AC
Department of Biochemistry, University of São Paulo, Brazil.
Chubatsu
L S
LS
Meneghini
R
R
D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D002999:Clone Cells / Q000187:drug effects / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000494:pharmacology
D002104:Cadmium; D013481:Superoxides; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D010100:Oxygen