Immunohistochemical identification of lysozyme and vimentin in an experimental malignant fibrous histiocytoma.
The immunoperoxidase avidin-biotin-peroxidase complex method was used to investigate the presence of histiocyte markers such as lysozyme, alpha-1-antitrypsin (A1AT) and alpha-1-anti-chymotrypsin (A1ACT) and of vimentin, a specific marker for mesenchymal differentiation, in a spontaneous and transplantable rat tumor of supposed fibroblastic-histiocytic origin. Positive staining was obtained for lysozyme and vimentin but A1AT and A1ACT were not demonstrable in any of the tumor sections. These results provide evidence for the fibro-histiocytic nature of the tumor studied and suggest its classification as a malignant fibrous histiocytoma (MFH).
Ribeiro
R A
RA
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Brasil.
Fonteles
M C
MC
Zucoloto
S
S
Bacchi
C E
CE
D000818:Animals; D018219:Histiocytoma, Benign Fibrous / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D009113:Muramidase / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014746:Vimentin / Q000378:metabolism*; D000514:alpha 1-Antichymotrypsin / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000378:metabolism
D014746:Vimentin; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D009113:Muramidase