title: T cell regulation of the thymus-independent antibody response to trinitrophenylated-Brucella abortus (TNP-BA).
abstract: We have previously observed a reduction of the T cell-dependent primary antibody response to dinitrophenylated keyhole limpet hemocyanin, and an enhancement of the T cell-independent response to trinitrophenylated Brucella abortus (TNP-BA) in BALB/c mice after treatment with total lymphoid irradiation (TLI). To elucidate the relative contribution of T and B cells to the enhanced T cell-independent antibody responses after TLI, a syngeneic primary adoptive transfer system was utilized whereby irradiated hosts were reconstituted with unfractionated spleen cells or a combination of purified T and B cells from TLI-treated and untreated control mice. Antibody responses of purified splenic B cells from TLI-treated BALB/c mice (TLI/B) to TNP-BA were enhanced 10-fold as compared with those of unfractionated (UF) spleen cells or B cells from normal (NL) BALB/c mice (NL/UF and NL/B, respectively). Splenic T cells from normal animals (NL/T) suppressed the anti-TNP-BA response of TLI/B by more than 100-fold. NL/T neither suppressed nor enhanced the response of NL/B. On the other hand, T cells from TLI-treated mice (TLI/T) enhanced by 100-fold the anti-TNP-BA response of NL/B, but neither suppressed nor enhanced the response of TLI/B. Thus, T cells can regulate the "T cell-independent" antibody response to TNP-BA. However, experimental manipulation of the T and B cell populations is needed to demonstrate the regulatory functions.
authors.forename: A
authors.initials: A
authors.lastname: Tanay
authors.forename: S
authors.initials: S
authors.lastname: Strober
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000955:Antigens, T-Independent / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology / Q000528:radiation effects / Q000637:transplantation; D002004:Brucella Vaccine / Q000276:immunology*; D002469:Cell Separation; D005260:Female; D007116:Immunization, Passive; D008221:Lymphoid Tissue / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009578:Nitrobenzenes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D014301:Trinitrobenzenes / Q000276:immunology*; D014916:Whole-Body Irradiation
chemical_list: D000907:Antibodies, Bacterial; D000955:Antigens, T-Independent; D002004:Brucella Vaccine; D009578:Nitrobenzenes; D014301:Trinitrobenzenes