Increased concentration of calcium channel and intracellular free calcium in the acute phase of deoxycorticosterone acetate salt hypertension.
Cardiac calcium channels and platelet Ca2+ concentrations were examined in deoxycorticosterone acetate (DOCA)-salt hypertension in rats to clarify the role of calcium channels in the regulation of intracellular Ca2+. Calcium channels were determined by the radioligand binding method and platelet Ca2+ concentrations were measured by a fluorescent Ca2+ indicator, Quin-2. The number of cardiac calcium channels was increased in DOCA-salt hypertension compared with controls 10 days after nephrectomy. Platelet Ca2+ concentrations were also increased in the acute phase of DOCA-salt hypertension. No significant changes were observed in the number of cardiac calcium channels and in platelet Ca2+ concentrations in the chronic phase of DOCA-salt hypertension. These results suggest that the increased platelet Ca2+ concentrations are induced by the increase in the number of calcium channels in the acute phase of hypertension.
Fukuda
K
K
Department of Medicine, Wakayama Medical College, Japan.
Baba
A
A
Kuchii
M
M
Nakamura
Y
Y
Nishio
I
I
Masuyama
Y
Y
D015720:5'-Nucleotidase; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D001835:Body Weight; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology; D003900:Desoxycorticosterone / Q000633:toxicity*; D006973:Hypertension / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D009708:Nucleotidases / Q000378:metabolism; D051381:Rats
D015220:Calcium Channels; D003900:Desoxycorticosterone; D009708:Nucleotidases; D015720:5'-Nucleotidase; D002118:Calcium