Autoradiographic localization of insulin-like growth factor II receptors in cerebellar cortex of weaver and Purkinje cell degeneration mutant mice.
Autoradiography was used to visualize insulin-like growth factor II (IGF-II) receptors in the cerebellar cortex of weaver and Purkinje cell degeneration (pcd) mice. These mutants were selected for their respective absence of granule or Purkinje cells. Histological preparations confirmed a severe loss of granule cells in the cerebella of weaver mutants and an absence of Purkinje cells in those of pcd mutants. Autoradiographs showed specific IGF-II binding to the granule cell layer of the cerebellar cortex in control mice, and in pcd mutants. No specific [125I]human IGF-II binding was observed in the cerebellar cortex of weaver mutants. These studies suggest that specific IGF-II receptor sites are located on the granule cells of the cerebellum.
Mendelsohn
L G
LG
Central Nervous System Research, Eli Lilly and Company, Indianapolis, IN 46285.
Smith
M C
MC
Lucaites
V L
VL
Kerchner
G A
GA
Ghetti
B
B
D000818:Animals; D001345:Autoradiography; D002525:Cerebellar Cortex / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D007457:Iodine Radioisotopes; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000378:metabolism*; D009410:Nerve Degeneration; D011689:Purkinje Cells / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017451:Receptors, Somatomedin; D012016:Reference Values; D013002:Somatomedins / Q000378:metabolism*; D013045:Species Specificity
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II