title: The role of operator position in SV40 T-antigen-mediated repression.
abstract: We have manipulated positional relationships between the SV40 early operator and promoter to study the repression of transcription by T antigen. Single or multiple insertions of T-antigen-binding region I resulted in only weak repression even when the operator was placed immediately adjacent to known promoter elements. The low levels of repression appear to reflect the intrinsic strength of the T-antigen-operator interaction.
authors.affiliation: Department of Microbiology, State University of New York, Stony Brook 11794.
authors.forename: M
authors.initials: M
authors.lastname: Cox
authors.forename: K
authors.initials: K
authors.lastname: Ryder
authors.forename: S
authors.initials: S
authors.lastname: Silver
authors.forename: P
authors.initials: P
authors.lastname: Tegtmeyer
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000235:genetics; D009875:Operator Regions, Genetic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D013489:Suppression, Genetic; D013696:Temperature; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D000952:Antigens, Polyomavirus Transforming; D015500:Chloramphenicol O-Acetyltransferase