title: Immunological properties of an N-terminal fragment of herpes simplex virus type 1 glycoprotein D expressed in Escherichia coli.
abstract: The N-terminal fragment, comprising residues -5 to 55 of herpes simplex virus type 1 glycoprotein D was expressed as a beta-galactosidase fusion protein in Escherichia coli. This gD-fusion protein reacts with monoclonal antibody LP 14 directed against glycoprotein D of HSV. Antisera obtained after immunization of rabbits with purified gD-fusion protein react with HSV-1 gD in a Western blot and with N-terminal synthetic peptides of gD. In addition, these antisera are able to neutralize viral infectivity in vitro.
authors.affiliation: Laboratorium voor Medische Microbiologie, Rijksuniversiteit Groningen, The Netherlands.
authors.forename: C H
authors.initials: CH
authors.lastname: Kocken
authors.forename: H J
authors.initials: HJ
authors.lastname: Geerligs
authors.forename: C A
authors.initials: CA
authors.lastname: Bos
authors.forename: G
authors.initials: G
authors.lastname: Ab
authors.forename: W J
authors.initials: WJ
authors.lastname: Weijer
authors.forename: J W
authors.initials: JW
authors.lastname: Drijfhout
authors.forename: G W
authors.initials: GW
authors.lastname: Welling
authors.forename: S
authors.initials: S
authors.lastname: Welling-Wester
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D002682:Chinchilla; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; D001616:beta-Galactosidase