Effects of acute stress, (1-24)ACTH administration and changes in superfusion temperature and flow rate on the in vitro secretion of glucocorticosteroids and aldosterone from the Mongolian gerbil (Meriones unguiculatus) adrenal gland.
The release of glucocorticosteroids and aldosterone rapidly decreased after start of superfusion and reached a steady base-line within 60-90 min of superfusion. While secretion markedly varied between experiments, it was very constant in the same experiment (coefficient of variation: 7.4-2.2% for glucocorticosteroids and 5.8-3.9% for aldosterone). After repeated exposure of adrenal tissue to 1 IU/ml (1-24)ACTH, glucocorticosteroid release progressively increased; under the same conditions aldosterone secretion was not changed. Glucocorticosteroid secretion from glands of animals stressed by 1-hr confinement or of animals injected with 6 IU (1-24)ACTH was significantly higher than that of controls over the 60-min superfusion period. Aldosterone secretion was not affected significantly by these pretreatments. After reduction of temperature from 35 to 1 degrees C, steroid release ceased. Elevation of temperature from 12 to 32 degrees C resulted in a linear increase of glucocorticosteroid and aldosterone secretion. A highly significant positive correlation was found between glucocorticosteroid and aldosterone amounts secreted from adrenals superfused at temperatures between 1 and 35 degrees C (r = 0.91, n = 116, P less than 0.0001). Changes of flow rate from 0.5 to 1.5 ml/min for 5 min induced a short term (1 min) stimulation of glucocorticosteroid and aldosterone release; reduction of flow rate to 0.5 ml/min for 5 min drastically diminished secretion of steroids below control levels for 1 min.
Fenske
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D000208:Acute Disease; D000302:Adrenal Cortex / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives*; D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D003366:Cosyntropin / Q000494:pharmacology*; D005849:Gerbillinae / Q000378:metabolism*; D005938:Glucocorticoids / Q000378:metabolism*; D066298:In Vitro Techniques; D013312:Stress, Physiological / Q000378:metabolism*; D013696:Temperature
D005938:Glucocorticoids; D003366:Cosyntropin; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone