Separation of amino acid and amide nitrogen from plant extracts for 15N analysis.
A rapid method was developed to obtain nitrogen for 15N analysis of individual amino acids and amides from plant tissue extracts. Amino or amide nitrogen was recovered as ammonia, suitable for preparation of samples for 15N emission spectrometry, using a combination of ion-exchange chromatography and distillation.
Ta
T C
TC
Joy
K
K
D000577:Amides / Q000302:isolation & purification*; D000596:Amino Acids / Q000302:isolation & purification*; D000641:Ammonia / Q000032:analysis; D001216:Asparagine / Q000302:isolation & purification; D001224:Aspartic Acid / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D007887:Fabaceae / Q000032:analysis; D005971:Glutamates / Q000302:isolation & purification; D018698:Glutamic Acid; D005973:Glutamine / Q000302:isolation & purification; D009584:Nitrogen / Q000302:isolation & purification*; D009587:Nitrogen Isotopes; D010944:Plants / Q000032:analysis*; D010946:Plants, Medicinal
D000577:Amides; D000596:Amino Acids; D005971:Glutamates; D009587:Nitrogen Isotopes; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D001216:Asparagine; D000641:Ammonia; D009584:Nitrogen