Inactivation of D-(-)-beta-hydroxybutyrate dehydrogenase by modifiers of carboxyl and histidyl groups.
Data presented in this paper suggest that D-(-)-beta-hydroxybutyrate dehydrogenase (BDH) purified from bovine heart mitochondria contains an essential carboxyl group and an essential histidyl residue at or near the active site. Lactate and malate dehydrogenases, which catalyze reactions analogous to that catalyzed by BDH, also contain an aspartyl and a histidyl residue at the active site [Birktoft, J.J., & Banaszak, L.J. (1983) J. Biol. Chem. 258, 472-482]. In addition, all three enzymes contain an essential arginyl residue, apparently concerned with electrostatic interaction with their respective carboxylic acid substrates, and promote ternary adduct formation involving the enzyme, NAD, and sulfite.
Prasad
P V
PV
Hatefi
Y
Y
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D001120:Arginine; D002417:Cattle; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004789:Enzyme Activation; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D006639:Histidine; D006884:Hydroxybutyrate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology*; D010743:Phospholipids / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D000317:Adrenergic alpha-Antagonists; D010743:Phospholipids; D011804:Quinolines; D006639:Histidine; D004024:Dicyclohexylcarbodiimide; C000470:EEDQ; D001120:Arginine; D006884:Hydroxybutyrate Dehydrogenase; D005022:Ethyldimethylaminopropyl Carbodiimide