title: Cyclic AMP induces metallothionein gene expression in rat hepatocytes but not in rat kidney.
abstract: Cyclic AMP analogues induced expression of metallothionein-I (MT-I) mRNA in adult rat liver and in rat hepatocytes cultured in serum-free medium. This induction occurred via an increased rate of transcription of the MT-I gene. The effect of cyclic AMP analogues was tissue-specific since no induction of MT-I mRNA occurred in rat kidney. Induction of MT-I mRNA by a combination of cyclic AMP analogue and dexamethasone was additive in liver and cultured hepatocytes, indicating that induction occurred via independent regulatory pathways.
authors.affiliation: Department of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, PA 15261.
authors.forename: V L
authors.initials: VL
authors.lastname: Nebes
authors.forename: D
authors.initials: D
authors.lastname: DeFranco
authors.forename: S M
authors.initials: SM
authors.lastname: Morris
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008668:Metallothionein / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013873:Thionucleotides / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D013873:Thionucleotides; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D003994:Bucladesine; D003907:Dexamethasone; D008668:Metallothionein; D000242:Cyclic AMP