title: Human papillomavirus in dysplasia and carcinoma of the cervix in Singapore.
abstract: A prospective study was conducted in Singapore in 1985 where 107 women with abnormal cervical smears were studied for cervical neoplasia and its association with the human papillomaviruses (HPV), using HPV 11, 16 and 18 DNA as probes. Cervical biopsies were performed for histology as well as for DNA Southern Blot hybridization studies to detect the presence of HPV 11, 16 or 18 genome. The prevalence of the various types of papillomavirus DNA in cervical tissue samples from cervical carcinoma and dysplasias is presented. HPV types 16 and 18 were found in 5 of 8 (63%) cases of invasive squamous cell carcinoma (SCC), in 12 of 41 (29%) cases of squamous carcinoma-in-situ (CIN 3) and in 3 of 48 (6%) cases of lower grade dysplasia. None of our cases of SCC were associated with unknown HPV types detected using a mixture of HPV 16 or 18 DNA under conditions of low stringency. These unknown HPV types were present in approximately one quarter of our cases of dysplasia of all grades.
authors.affiliation: Referenzzentrum für humanpathogene Papillomviren/ATV im Deutschen Krebsforschungszentrum, Federal Republic of Germany.
authors.forename: S K
authors.initials: SK
authors.lastname: Lim-Tan
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: I T
authors.initials: IT
authors.lastname: Sng
authors.forename: E M
authors.initials: EM
authors.lastname: de Villiers
authors.forename: H
authors.initials: H
authors.lastname: zur Hausen
authors.forename: T H
authors.initials: TH
authors.lastname: Ho
authors.forename: T
authors.initials: T
authors.lastname: Yoong
mesh_terms: D000328:Adult; D015139:Blotting, Southern; D002278:Carcinoma in Situ / Q000453:epidemiology / Q000382:microbiology*; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000382:microbiology*; D015344:DNA Probes, HPV; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification; D011446:Prospective Studies; D012846:Singapore; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000382:microbiology*; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000382:microbiology*; D014626:Vaginal Smears
chemical_list: D015344:DNA Probes, HPV