[Measurements of the time of rotational correlation of bovine serum albumin molecule using iodine and mercury-containing spin-labels].
New spin-labels based on iodine and hydrargirum containing imidazolids were approbated on the bovine serum albumin (BSA) molecule. It is shown that all hydrargirum labels are binded to the external SH-group of BSA practically immediately in comparison with earlier known spin-labels based on piperidine with maleimide and iodacetamide groups, requiring some minutes of hours, correspondingly. Rotational correlation times and the character of relative mobility of the spin-label were measured. Values of the rotational correlation times of the protein molecule obtained by hydrargirum containing labels were found in the range of correlation times typical for the BSA molecule and represent at pH 7.0 a rigid stretch ellipsoid of rotation. The result obtained revealed that the relative reorientation nature of spin-labels is essentially different and is taken into account in different values of order parametres S according to model--fast anisotropic rotator on slow isotropic rotator. ESR spectra based on theoretical calculations by means of computer are given.
Arutiunian
A E
AE
Dudich
I V
IV
Timofeev
V P
VP
D003198:Computer Simulation; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles; D007455:Iodine; D008628:Mercury; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013113:Spin Labels; D013438:Sulfhydryl Compounds / Q000032:analysis; D014783:Viscosity
D007093:Imidazoles; D013113:Spin Labels; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D007455:Iodine; D008628:Mercury
Izmerenie vremeni vrashchatel'noĭ korreliatsii molekuly bych'ego syvorotochnogo al'bumina s pomoshch'iu ĭod- i rtut'soderzhashchikh spin-metok.