Liquid chromatographic determination of L-ascorbate 2-polyphosphate in fish feeds by enzymatic release of L-ascorbate.
An accurate method was devised to assay L-ascorbic 2-polyphosphate esters (AsPP) in fish feed by phosphatase digestion followed by determination of the released L-ascorbic acid (AsA). Compressed yeast and dithiothreitol are added to the phosphatase reaction mixture to give 95-100% recovery of AsA, which is quantitated by reverse-phase liquid chromatography (LC) with electrochemical detection. Chromatograms of all feed digests showed baseline resolution of AsA. In 3 feeds, to which 75-125 ppm AsA equivalents in the form of AsPP were added, the assay procedure gave 98-100% recovery of AsA.
Wang
X Y
XY
Kansas State University, Department of Grain Science and Industry, Manhattan 66506.
Liao
M L
ML
Hung
T H
TH
Seib
P A
PA
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis*; D002853:Chromatography, Liquid; D005399:Fishes; D006868:Hydrolysis; D010744:Phosphoric Monoester Hydrolases; D011122:Polyphosphates / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D011122:Polyphosphates; D010744:Phosphoric Monoester Hydrolases; D001205:Ascorbic Acid