Clonospecific structural heterogeneity in the Thy-1 molecule from mouse T lymphocytes.
The Thy-1 molecule immunoprecipitated from detergent-solubilized, 125I-labeled cell-surface proteins was shown to be processed in two distinct ways by mouse T lymphocytes: one leading to the expression by thymocytes, concanavalin A-activated spleen blasts, and six of nine T-cell clones of a molecule of 25-28 kd, and another, observed in three other T-cell clones, leading to the expression at their surface of a so far undescribed low Mr (23 kd) form of Thy-1. The results of two-dimensional gel electrophoresis and neuraminidase, endoglycosidase H, and endoglycosidase F treatment revealed that the observed heterogeneity of Thy-1 molecules from peripheral cloned T cells was due to major differences in the maturation and sialylation of their N-linked complex-type oligosaccharide residues. It was also found that a given T-cell clone could express T200, LFA.1, and transferrin receptor molecules with a low or high Mr. Furthermore, and in contrast to previously reported results, this study revealed that the differences in cell-surface glycoprotein profiles could not be correlated with the Lyt-2,3/T4 phenotypes, the specificity for allo-H-2, allo-I-A, allo-I-E, or GAT + I-Ak determinants, nor with the cytolytic or helper/amplifier potential of the various T-cell clones examined. The possible implications of these findings are discussed.
Pont
S
S
Van Agthoven
A
A
Naquet
P
P
Pierres
A
A
Schmitt-Verhulst
A M
AM
Pierres
M
M
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D007120:Immunochemistry; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D006023:Glycoproteins; D008565:Membrane Proteins; D018800:Thy-1 Antigens