title: Clonal analysis of early mammalian development.
abstract: Various extrinsic markers have been used to label single cells in the early mouse embryo. However, they are appropriate only for short-term experiments because of their susceptibility to dilution. Studies on cell lineage and commitments have therefore depended mainly on exploiting genes as markers by combining cells from embryos that differ in genotype at particular loci. Tissue recombination and transplantation experiments using such indelible intrinsic markers have enabled the fate of different cell populations in the blastocyst to be determined with reasonable precision. The trophectoderm and inner cell mass (i.c.m.) give rise to distinct complementary groups of tissues in the later conceptus, as do the primitive endodermal and primitive ectodermal components of the more mature i.c.m. When cloned by blastocyst injection, single i.c.m. cells colonize only those parts of host conceptuses that are derived from their tissue of origin. Thus, while clonal descendants of early i.c.m. cells can contribute to all tissues other than those of trophectodermal origin, primitive endodermal and primitive ectodermal clones are restricted, respectively, to the extraembryonic endoderm versus all i.c.m. derivatives except the extraembryonic endoderm. Interestingly, individual primitive ectoderm cells can include both germ cells and somatic cells among their mitotic descendants. By using the genetically determined presence versus absence of cytoplasmic malic enzyme activity as a cell marker, the deployment of clones has been made visible in situ in whole-mount preparations of extraembryonic membranes. Very little mixing of donor and host cells was seen in either the endoderm of the visceral yolk sac or the mesodermal and ectodermal layers of the amnion. In contrast, mosaicism in the parietal endoderm was so fine grained that, in all except 1 of 15 fields from several specimens that were analysed, the arrangement of donor and host cells did not differ significantly from that expected on the basis of their random association.
authors.forename: R L
authors.initials: RL
authors.lastname: Gardner
mesh_terms: D000650:Amnion / Q000166:cytology / Q000502:physiology; D000818:Animals; D001757:Blastomeres / Q000201:enzymology; D002454:Cell Differentiation; D002678:Chimera; D002999:Clone Cells / Q000166:cytology / Q000201:enzymology / Q000502:physiology; D004622:Embryo, Mammalian / Q000166:cytology* / Q000201:enzymology / Q000502:physiology; D004707:Endoderm / Q000502:physiology; D005819:Genetic Markers; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D008291:Malate Dehydrogenase / Q000235:genetics; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D051379:Mice; D051381:Rats; D013045:Species Specificity; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
chemical_list: D005819:Genetic Markers; D007527:Isoenzymes; D008291:Malate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase