title: In vivo properties of a cloned K88 adherence antigen determinant.
abstract: An Escherichia coli strain of serotype O9:K36:H19 harboring the K88 recombinant plasmid pMK005 was able to efficiently colonize the small bowel of young piglets after oral infection. The strain expressed K88 antigen in vivo, and bacteria were detected in close association with the surface of the intestinal villi. Mice infected orally or intravenously with attenuated Salmonella typhimurium SL3261 harboring pMK005 were well protected against subsequent challenge with the highly virulent S. typhimurium SL1344. Anti-K88 antibodies were detected in the serum of mice immunized with SL3261(pMK005).
authors.forename: G
authors.initials: G
authors.lastname: Dougan
authors.forename: R
authors.initials: R
authors.lastname: Sellwood
authors.forename: D
authors.initials: D
authors.lastname: Maskell
authors.forename: K
authors.initials: K
authors.lastname: Sweeney
authors.forename: F Y
authors.initials: FY
authors.lastname: Liew
authors.forename: J
authors.initials: J
authors.lastname: Beesley
authors.forename: C
authors.initials: C
authors.lastname: Hormaeche
mesh_terms: D018830:Adhesins, Escherichia coli; D000818:Animals; D000831:Animals, Newborn; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001428:Bacterial Vaccines / Q000276:immunology; D003001:Cloning, Molecular; D003967:Diarrhea / Q000382:microbiology* / Q000517:prevention & control; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D010861:Fimbriae, Bacterial; D005786:Gene Expression Regulation; D007422:Intestines / Q000382:microbiology; D051379:Mice; D008970:Molecular Weight; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology; D013552:Swine; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated