Pathogenesis of acute myocardial necrosis in inbred mice infected with coxsackievirus B3.
The pathogenesis of myocardial necrosis due to CB3W infection was studied in BALB/c and C3H/HeJ mice. BALB/c mice infected with 5 x 10(4) pfu were found to die of massive hepatic coagulative necrosis before myocardial changes occurred. Reducing the inoculum size to 5 x 10(2) pfu resulted in sublethal hepatic involvement and multifocal myocardial coagulative necrosis by day 7 p.i. In contrast, C3H/HeJ mice survived infection and developed multifocal myocardial coagulative necrosis, but not liver disease following inoculation with as much as 5 x 10(6) pfu of CB3W. As with BALB/c mice infected with 5 x 10(2) pfu, myocardial lesions became apparent in C3H/HeJ mice a few days after peak cardiac virus titer was attained. Minimal inflammatory infiltrate was seen following development of cellular necrosis and was restricted to the areas of virus-induced pathologic change. However, no evidence was found for virus-specific cytotoxic T cell activity or for delayed type hypersensitivity responses. Furthermore, myocardial necrosis in CB3W-infected, T cell-depleted C3H/HeJ mice was as severe as in CB3W-infected, immunocompetent mice. These data have led us to conclude that cardiac lesions were due to virus-induced cytopathology rather than immunopathogenic mechanisms.
Grun
J B
JB
Department of Microbiology and Immunology, Hahnemann University School of Medicine, Philadelphia, PA 19102.
Schultz
M
M
Finkelstein
S D
SD
Crowell
R L
RL
Landau
B J
BJ
D000818:Animals; D009202:Cardiomyopathies / Q000382:microbiology* / Q000473:pathology; D003384:Coxsackievirus Infections / Q000276:immunology / Q000473:pathology*; D004195:Disease Models, Animal; D029822:Enterovirus B, Human; D006968:Hypersensitivity, Delayed / Q000209:etiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009336:Necrosis / Q000209:etiology; D011392:Proline; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D011392:Proline