Screening of anti-HTLV antibody in sera of normal individuals and patients with malignancies in Taiwan.
In the present study an immunofluorescence using KH-2 cells as target cells, has been developed for the screening of 1200 serum samples from normal individuals and 450 of cases from patients with various malignancies. The positive anti-HTLV-I antibody rate in the former group is 0.083% (1/1200) and while in the latter it is found to be 1.8% (8/450) (including 3 adult T-cell leukemia/lymphoma cases of the 92 hematopoietic and 5 of 358 non-hematopoietic malignancies). The differences between the two groups are found to be significantly different (p value is less than 0.0001). In addition to the 3 adult T-cell leukemia/lymphoma cases, the 5 seropositive cancer patients are of 5 different diseases. We have searched for the adult T-cell leukemia virus antigen and the p19 core protein in lymphoid cells of seropositive persons and the only positive cases were from cells of two proven adult T-cell leukemia (ATL) patients. Our results suggest that Taiwan is not an endemic area of adult T-cell leukemia virus and that KH-2 cells may be used for the detection of anti-HTLV-I antibodies.
Chen
P M
PM
Chiu
C F
CF
Chiou
T J
TJ
Tzeng
C H
CH
Lin
S T
ST
Wang
L S
LS
Lee
Y
Y
Chien
K Y
KY
Chang
S S
SS
Maeda
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D015480:Deltaretrovirus Antibodies; D005260:Female; D015488:HIV Antigens; D006801:Humans; D007938:Leukemia / Q000276:immunology / Q000382:microbiology; D008223:Lymphoma / Q000276:immunology / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology* / Q000382:microbiology; D013624:Taiwan; D014758:Viral Core Proteins / Q000032:analysis
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015480:Deltaretrovirus Antibodies; D015488:HIV Antigens; D014758:Viral Core Proteins