Cholecystokinin octapeptide-evoked [3H]acetylcholine release from guinea pig gallbladder.
Cholecystokinin-octapeptide (CCK-OP) produced a contractile response in isolated guinea pig gallbladder; the response consisted of scopolamine-sensitive and scopolamine-insensitive components, neither of which were affected by tetrodotoxin or hexamethonium. In the presence of tetrodotoxin, CCK-OP evoked [3H]acetylcholine (ACh) release from strips of gallbladder in a concentration-dependent manner. These results suggest that CCK-OP acts at two sites in the guinea pig gallbladder, viz. the smooth muscle cell and the postganglionic cholinergic nerve terminal.
Yamamura
T
T
Takahashi
T
T
Kusunoki
M
M
Kantoh
M
M
Ishikawa
Y
Y
Utsunomiya
J
J
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005704:Gallbladder / Q000378:metabolism*; D006168:Guinea Pigs; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009994:Osmolar Concentration; D012601:Scopolamine / Q000494:pharmacology; D012844:Sincalide / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000494:pharmacology; D014316:Tritium
D006584:Hexamethonium Compounds; D014316:Tritium; D018738:Hexamethonium; D013779:Tetrodotoxin; D012601:Scopolamine; D012844:Sincalide; D000109:Acetylcholine