title: Interferon inhibits herpes simplex virus-specific translation: a reinvestigation.
abstract: Reports on the arrest of herpes simplex virus type 1 (HSV-1) replication by interferon (IFN) are inconsistent. By the use of immunofluorescence and immunoblot assays with monoclonal and polyclonal antibodies, effective arrest of viral translation by human IFN-alpha in human fibroblasts was detected for the HSV-1 strains KOS and McIntyre. In HeLa cells which are less sensitive to IFN inhibition and in 444 cells, a HeLa-fibroblast hybrid cell line, the inhibition was less pronounced. These results confirm earlier observations that IFN or polyinosinic.polycytidylic acid block the replication of HSV-1 in human, monkey and mouse cells no later than the immediate early phase of infection.
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene der Universität, Freiburg, F.R.G.
authors.forename: B
authors.initials: B
authors.lastname: Altinkilic
authors.forename: G
authors.initials: G
authors.lastname: Brandner
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors; D011994:Recombinant Proteins; D018139:Simplexvirus / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D007370:Interferon Type I; D011500:Protein Synthesis Inhibitors; D011994:Recombinant Proteins; D014764:Viral Proteins