title: Properties of allelic variants of phosphoglucomutase from the sea anemone Metridium senile.
abstract: The phosphoglucomutase (Pgm) locus from populations of the sea anemone Metridium senile has three alleles in natural populations from the northeastern coast of North America. Two of the alleles exhibit clinal variation north of Cape Cod, suggesting a possible association of allele frequency with environmental temperature. This clinal pattern is reproducible and stable over at least brief periods of time. The allozymes encoded by each of the six Pgm genotypes have been partially purified and characterized. The symmetrical pH optimum for Vmax is pH 7.5; the apparent Km (Kmapp) of glucose-1-phosphate declines monotonically as the pH increases from 6.5 to 8.5. There are no pronounced differences in heat stabilities of PGM produced by various genotypes, nor are there significant differences in specific activities. There are no differences in the sensitivity of Vmax to temperature. Kmapp values are very low for all genotypes, ranging from about 2 to 12 microM, depending upon the temperature. Kmapp of glucose-1-phosphate declines as the temperature is raised for all genotypes, whether the pH is held constant or allowed to vary with the temperature. Under certain conditions, there are small significant differences among genotypes in Kappm values, but there is no systematic pattern to these differences. The present data provide no biochemical explanation for the maintenance of the Pgm cline by selection for functional differences under different thermal regimes.
authors.forename: R J
authors.initials: RJ
authors.lastname: Hoffmann
mesh_terms: D000483:Alleles; D000818:Animals; D003063:Cnidaria / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010733:Phosphoglucomutase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012615:Sea Anemones / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
chemical_list: D010733:Phosphoglucomutase