Effects of chlorphentermine and nitrogen dioxide on murine alveolar macrophages.
Male Swiss-Webster mice were treated daily for 14 days with either 120 mg/kg chlorphentermine (CP) to produce pulmonary lipidosis or an equal volume of water. Animals in each treatment group were then exposed by whole-body inhalation to either air or NO2 for 48 h. Immediately following exposure, alveolar macrophages (MPs) were collected from each animal by bronchoalveolar lavage. Assays performed on adherent viable MPs showed some changes in metabolic reduction, phagocytosis, and killing activity. 5'-Nucleotidase activity and yeast phagocytosis and killing assays suggested that CP elicited an increase in phagocytosis over control levels. Although the percentage metabolic reduction and microbicidal killing activities following CP were not increased when compared to controls, absolute reduction and killing (percentage values times total MPs) were significantly increased. These increased functions seemed to be highly dependent on the large increase in the total number of MPs induced by CP. It is possible that the large accumulation of MPs in the airways of the lipidotic lung may help protect the alveolar epithelium from NO2 by quenching free radicals produced during NO2-induced lipid peroxidation.
White
S M
SM
Department of Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, Indiana 47907.
DeNicola
D B
DB
Rebar
A H
AH
Born
G S
GS
Kessler
W V
WV
D015720:5'-Nucleotidase; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002745:Chlorphentermine / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D009580:Nitroblue Tetrazolium; D009585:Nitrogen Dioxide / Q000494:pharmacology*; D009708:Nucleotidases / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D010645:Phentermine / Q000031:analogs & derivatives*; D012016:Reference Values
D009580:Nitroblue Tetrazolium; D010645:Phentermine; D009708:Nucleotidases; D015720:5'-Nucleotidase; D002745:Chlorphentermine; D009585:Nitrogen Dioxide