Radioreceptor assay for leukotriene B4. Use for determination of leukotriene B4 formation by whole human blood.
A crude membrane fraction from guinea-pig splenic tissue containing leukotriene (LT) B4-binding sites was prepared and used in a radioreceptor assay for the quantitative determination of LTB4 in clotting whole human blood as well as in eluates from reversed-phase HPLC. Binding of [3H]LTB4 to the binding sites was inhibited by 50% with 0.31 ng LTB4. The relative cross-reaction of 20-OH-LTB4, 20-COOH-LTB4 and 12-hydroxyeicosatetraenoic acid (HETE) was 9.51%, 0.41% and 0.03%, respectively. The relative cross-reaction of various other prostanoids, cysteinyl-LT, mono-HETE's and arachidonic acid was below 0.01%. The spleen binding sites are useful for a rapid, simple and specific quantification of LTB4 in biological material.
Simmet
T
T
Department of Pharmacology and Toxicology, Ruhr-University Bochum, Federal Republic of Germany.
Luck
W
W
D000818:Animals; D000001:Calcimycin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006168:Guinea Pigs; D006801:Humans; D007975:Leukotriene B4 / Q000096:biosynthesis / Q000097:blood*; D008297:Male; D009637:Masoprocol / Q000494:pharmacology; D011869:Radioligand Assay
D007975:Leukotriene B4; D000001:Calcimycin; D009637:Masoprocol