31P magnetization transfer in the phosphoglyceromutase-enolase coupled enzyme system.
The rate of exchange of phosphoryl groups between 2-phosphoglycerate, 3-phosphoglycerate and phosphoenolpyruvate by the coupled phosphoglyceromutase-enolase enzyme system using one- and two-dimensional 31P NMR spectroscopy was measured. Magnetization exchange in one-dimensional experiments was achieved by saturation transfer with selective irradiation at both one and two sites in this three-site exchange system using the DANTE pulse sequence. The two-dimensional magnetization exchange experiment avoids the need to selectively saturate at one or more frequencies which may be difficult in complex exchange systems. Analysis of the two-dimensional exchange experiment by the back transformation method yielded exchange rate constants in good agreement with the saturation transfer method.
Chapman
B E
BE
Department of Biochemistry, University of Sydney, NSW, Australia.
Stewart
I M
IM
Bulliman
B T
BT
Mendz
G L
GL
Kuchel
P W
PW
D000818:Animals; D005988:Glyceric Acids / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010736:Phosphoglycerate Mutase / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011817:Rabbits
D005988:Glyceric Acids; D010710:Phosphates; C008885:2-phosphoglycerate; D010728:Phosphoenolpyruvate; C005156:3-phosphoglycerate; D010770:Phosphotransferases; D010751:Phosphopyruvate Hydratase; D010736:Phosphoglycerate Mutase