title: An E. coli-yeast shuttle cosmid with positive selection for inserted fragments.
abstract: We describe the construction of a cosmid cloning vector, pMT555, which allows positive selection for the presence of an inserted DNA fragment. The vector contains sequences which enable its replication and selection in either E. coli or Saccharomyces cerevisiae. We demonstrate that pMT555 may be used for the efficient construction of total genomic banks from small quantities of donor DNA. The positive selection permits the stable maintenance of the cosmid in E. coli and the faithful replication of inserted sequences.
authors.affiliation: Department of Biochemistry and Applied Molecular Biology, University of Manchester, UK.
authors.forename: M E
authors.initials: ME
authors.lastname: Gent
authors.forename: P
authors.initials: P
authors.lastname: Crowley
authors.forename: J R
authors.initials: JR
authors.lastname: Ludwig
authors.forename: R
authors.initials: R
authors.lastname: Anwar
authors.forename: D A
authors.initials: DA
authors.lastname: Sugden
authors.forename: P F
authors.initials: PF
authors.lastname: Sims
authors.forename: S G
authors.initials: SG
authors.lastname: Oliver
mesh_terms: D003360:Cosmids; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012641:Selection, Genetic; D014170:Transformation, Genetic
chemical_list: D004251:DNA Transposable Elements