Specific lysis of noradrenergic synaptosomes by an antiserum to dopamine-beta-hydroxylase.
An antiserum to dopamine-beta-hydroxylase purified from bovine adrenal medulla, acting in the presence of complement, caused the release of 12% of lactate dehydrogenase, 20% of tyrosine hydroxylase activity, and 40% of noradrenaline (NA) content from synaptosomes prepared from rat brain cerebral cortex. Uptake of [3H]NA was reduced by 54%. Anti-serum alone or complement alone were without action. The antiserum plus complement had no effect on choline uptake and did not release choline acetyltransferase, glutamate decarboxylase, dopamine or 5-hydroxytryptamine. These results suggest selective lysis of noradrenergic terminals had occurred. An enhancement of lysis was not observed when synaptosomes were stimulated with 75 mequiv./lK+ and exposed to a sub-maximal dose of antiserum, plus complement.
Docherty
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M
Bradford
H F
HF
Joh
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D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology; D003165:Complement System Proteins / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism; D004299:Dopamine beta-Hydroxylase / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D013574:Synaptosomes / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D007106:Immune Sera; D012701:Serotonin; D003165:Complement System Proteins; D007770:L-Lactate Dehydrogenase; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D004298:Dopamine; D009638:Norepinephrine