Factors controlling the expression of the NGF receptor in PC12 cells.
An enzyme-linked immunoadsorbent assay (ELISA) has been used to study the relative expression of the nerve growth factor receptor (NGF-R) in PC12 cells. Both nerve growth factor (NGF) and fibroblast growth factor (FGF) were found to induce time-dependent increases in receptor expression. The former response was half-maximal at approximately 2 ng/ml and maximal at 25-50 ng/ml. The induction of NGF-R expression by NGF was associated with an increase in the relative level of a 3.8 kb mRNA species encoding this protein. Whereas the induction of the NGF-R by NGF and FGF were both fully inhibited by cholera toxin and cordycepin, the responses were differentially inhibited by the kinase inhibitor K-252a.
Doherty
P
P
Institute of Neurology, London, U.K.
Seaton
P
P
Flanigan
T P
TP
Walsh
F S
FS
D000818:Animals; D002227:Carbazoles / Q000494:pharmacology; D002772:Cholera Toxin / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D026121:Indole Alkaloids; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D002227:Carbazoles; D003839:Deoxyadenosines; D026121:Indole Alkaloids; D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D005346:Fibroblast Growth Factors; D002772:Cholera Toxin; C049985:staurosporine aglycone