Over-production and characterization of the nifA gene product of Klebsiella pneumoniae--the transcriptional activator of nif gene expression.
The nifA gene of Klebsiella pneumoniae, which encodes the transcriptional activator of nif gene expression, was cloned into a number of plasmid vectors to obtain high-level synthesis of nifA product (NifA). When over-produced, NifA was very insoluble and it precipitated with the cell debris after cell lysis. Localization of beta-galactosidase activity from a nifA-lacZ translational fusion confirmed the insoluble nature of NifA. Analysis of two translational fusions in which the last six C-terminal amino acids of NifA were deleted suggests that these residues are required for activity.
Tuli
R
R
AFRC Institute of Plant Science Research, University of Sussex, Brighton, UK.
Merrick
M J
MJ
D001426:Bacterial Proteins / Q000096:biosynthesis*; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013696:Temperature; D014158:Transcription, Genetic
D001426:Bacterial Proteins