Differences in the mechanism of induction of interferon-alpha by herpes simplex virus and herpes simplex virus-infected cells.
The cellular source of IFN alpha after induction with Herpes simplex virus type-1 (HSV) and HSV-infected fibroblasts was investigated by using human peripheral blood mononuclear cell (PBMC) populations, purified according to conventional procedures, and which included T- and B-lymphocytes as well as monocytes. It appears that the cells responding to HSV virions are monocytes, whereas the PBMC population induced by HSV-infected cells is represented by B-lymphocytes. Furthermore, by using monoclonal antibody (MoAb) to HLA class I and class II products, it appears that different membrane structures are involved in the induction of IFN by HSV virions, as opposed to HSV-infected cells. In fact, most anti-HLA class II MoAbs inhibit IFN induction by HSV-infected cells, and not IFN induction by HSV virions.
Capobianchi
M R
MR
Institute of Virology, University of Rome, Italy.
Malavasi
F
F
Di Marco
P
P
Dianzani
F
F
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D005347:Fibroblasts / Q000382:microbiology*; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000382:microbiology; D009000:Monocytes / Q000378:metabolism; D018139:Simplexvirus / Q000502:physiology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D007370:Interferon Type I