Effect of dimethylnitrosamine upon carbamoyl phosphate and ornithine utilization in rat liver.
Newborn CFY rats were intraperitoneally treated with a single dose of dimethylnitrosamine. Changes in the activity of carbamoyl-phosphate synthase I and II, ornithine carbamoyltransferase, aspartate carbamoyltransferase and dihydroorotase, i.e. utilization of ornithine and carbamoyl-phosphate pools were followed for 300 days. One or two months after treatment the activity of enzymes which utilize ornithine and carbamoyl-phosphate was enhanced in the cytosol, whereas the activity of the intramitochondrial enzymes in urea synthesis was decreased. Changes in the utilization of ornithine and carbamoyl-phosphate pools, characteristic of proliferating tissues, appeared to be irreversible.
Karsai
T
T
Rády
P
P
Nagy
Z
Z
Szondy
Z
Z
D000818:Animals; D000831:Animals, Newborn; D002219:Carbamates / Q000378:metabolism*; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000378:metabolism; D002221:Carbamyl Phosphate / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004080:Dihydroorotase / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D009952:Ornithine / Q000378:metabolism*; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D051381:Rats
D002219:Carbamates; D002221:Carbamyl Phosphate; D009952:Ornithine; D009954:Ornithine Carbamoyltransferase; D004080:Dihydroorotase; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing); D004128:Dimethylnitrosamine