Screening extracts of Euphorbia garuana N.E.Br. for in-vitro cytotoxicity.
Two biological assays utilising in-vitro uptake of 3H-thymidine into TLX/5 lymphoma and rat basophilic leukaemia cells have been used for rapid detection and subsequent isolation of active compounds from the latex of Euphorbia garuana (Euphorbiaceae). Two potent esters were characterized from the latex of E. garuana reportedly used in traditional medicine for the treatment of certain forms of cancer.
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Department of Pharmacognosy, Faculty of Pharmacy, College of Medicine, University of Ibadan, Nigeria.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis*; D002470:Cell Survival; D055598:Chemical Phenomena; D002621:Chemistry; D004224:Diterpenes / Q000032:analysis / Q000302:isolation & purification*; D005260:Female; D015471:Leukemia, Basophilic, Acute / Q000473:pathology; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D010936:Plant Extracts / Q000032:analysis*; D010946:Plants, Medicinal / Q000032:analysis; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D010936:Plant Extracts; C059274:7-benzoylingol-3,8,12-triacetate; C059275:ingol-3,7,8,12-tetraacetate; D013936:Thymidine