Influence of ligation of pancreatic and bile ducts on the interaction of vasoactive intestinal peptide with rat duodenal epithelial cells.
The specific binding of vasoactive intestinal peptide (VIP) and the stimulatory effect of the neuropeptide on cyclic AMP in duodenal epithelial cells were modified 3 days following pancreaticobiliary exclusion. The binding capacity, but not the affinity, of VIP receptors decreased (by about 50%) as a consequence of the surgical manipulation. VIP was equally potent but showed a lower efficiency (about 45%) in stimulating cyclic AMP after ligation of the pancreatic and bile ducts. These observations may be either a consequence or a cause of the adaptive response of duodenal epithelium, the last possibility suggesting a role of VIP in the mechanisms of growth and differentiation of intestinal mucosa.
Díaz-Juárez
J L
JL
Arilla
E
E
Prieto
J C
JC
D000818:Animals; D001652:Bile Ducts / Q000502:physiology; D001667:Binding, Competitive; D004386:Duodenum / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005704:Gallbladder / Q000502:physiology*; D007700:Kinetics; D008297:Male; D010179:Pancreas / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D011956:Receptors, Cell Surface; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide