Microelectrode for measuring local cortical oxygen tension and blood flow in the same microareas of cat cortex.
The purpose of this study is to investigate the relationship between changes in cortical Po2 (bPo2) and cortical blood flow when inhalating different oxygen mixtures. Experiments were performed on 31 cats anaesthetized with N2O/O2 plus supplemental drugs for immobilization and analgesia. Cortical Po2 was measured by the polarographic method using 25 microns Pt electrode (coated) and a 250 micron Ag-AgCl2 indifferent electrode, especially designed to rest lightly on the cortex by means of coiled spring support. Using the same electrode but with opposite tip polarization; local cortical blood flow (lCoBF) was measured at the same site by the H2-clearance method. The change of bPo2 in going from FiO2 25% to FiO2 75% was measured at 58 different sites. (Table 2). At 25% FiO2 the mean bPo2 was 24 +/- 16 mm Hg, with a range from 4 to 92 mm Hg. With increased FiO2 to 75% local bPo2 responded quite differently at adjacent sites. At 75% FiO2 the mean bPo2 was 42.7 +/- 32 mm Hg with a range from 10 mm Hg to 198 mm Hg. The bPo2 and lCoBF were both measured at 44 of the 58 different sites. Cortical sites with a resting bPo2 greater than 30 mm Hg at 25% FiO2 had a lCBF = 57 +/- 12 ml/100g m; whereas sites with resting normoxic bPO2 less than 20 mm Hg had a mean ICoBF = 56 +/- 14 ml/100g min.(ABSTRACT TRUNCATED AT 250 WORDS)
Yonekura
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Austin
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D000770:Anesthesia, Endotracheal; D000818:Animals; D002415:Cats; D002540:Cerebral Cortex / Q000098:blood supply / Q000502:physiology*; D005260:Female; D008297:Male; D008839:Microelectrodes; D009482:Neurophysiology / Q000295:instrumentation / Q000379:methods*; D009609:Nitrous Oxide; D010100:Oxygen / Q000032:analysis*; D011048:Polarography / Q000295:instrumentation / Q000379:methods*
D009609:Nitrous Oxide; D010100:Oxygen