Mouse B cells do not proliferate in human interleukin 2.
By pre-activation with a monoclonal anti-IgM antibody coupled to Sepharose, either in the absence or presence of low doses of lipopolysaccharide, mouse B cell populations were rendered responsive to recombinant DNA derived human interleukin 2 (IL 2). However, if the B cell populations were subjected to separation based on their buoyant density before pre-activation, only low but not high buoyant density cells became responsive to IL 2. Both cell populations subsequent to anti-IgM pre-activation were equally responsive to a Sephadex G-100 fraction of supernatants from rat spleen cells stimulated with concanavalin A. Furthermore, the differences in the IL 2 titration curves on T and B cell populations indicate that only a subpopulation of cells are responding to IL 2 in the B cell populations. In B cell populations that did respond to IL 2, a population of Thy-1.2-positive cells appeared. The proportion of IL 2-responsive T cells needed to give a significant signal when admixed with nonresponding B cell populations was quantitated to be less than 1%.
Leanderson
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Julius
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D007075:Immunoglobulin M / Q000276:immunology; D007376:Interleukin-2; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D012685:Sepharose / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018800:Thy-1 Antigens; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D007075:Immunoglobulin M; D007376:Interleukin-2; D008070:Lipopolysaccharides; D018800:Thy-1 Antigens; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D014316:Tritium; D012685:Sepharose; D013936:Thymidine