Characterization of somatostatin binding sites in cytosolic fraction of pig retina.
Specific binding sites for somatostatin have been characterized in the cytosolic fraction of the pig retina. The interaction of 125I-Tyr11-somatostatin with cytosolic fraction was rapid, reversible, specific, saturable and temperature-dependent. At 15 degrees C, the binding data were compatible with the existence of two classes of binding sites: a high-affinity class with a Kd of 16.1 nM and a low-affinity class with a Kd of 236.4 nM. The presence of somatostatin binding sites in the cytosolic fraction of the retina, together with the known occurrence of somatostatin at this level, strongly suggest that this peptide may be involved in the physiology of the retina.
Colás
B
B
Arilla
E
E
Prieto
J C
JC
D000818:Animals; D001665:Binding Sites; D005591:Chemical Fractionation; D003600:Cytosol / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007457:Iodine Radioisotopes; D012160:Retina / Q000378:metabolism*; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D013552:Swine; D013997:Time Factors
D007457:Iodine Radioisotopes; D013004:Somatostatin; C032367:somatostatin, Tyr(11)-