Mutual competitive inhibition between target sites during the restriction endonuclease digestion of DNA.
The reaction rate of restriction endonuclease was evaluated theoretically, considering the competition between target sites and a nonspecific DNA. An equation for the initial cleavage rate at a single site for a DNA substrate containing more than one recognition site was derived. The consequences for the study of preferential cleavage were discussed.
Karlovský
P
P
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
D001667:Binding, Competitive; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological
D004247:DNA; D004262:DNA Restriction Enzymes