title: Thiourea and dimethylthiourea decrease human neutrophil bactericidal function in vitro.
abstract: Addition of thiourea (TU) or dimethylthiourea (DMTU) decreased killing of Staphylococcus aureus, 502A, and decreased concentrations of hydrogen peroxide (H2O2), and hydroxyl radical (.OH), but not superoxide anion (O2-.) or lysozyme concentrations, in mixtures containing human neutrophils in vitro. Addition of TU or DMTU also decreased concentrations of H2O2, .OH, or hypochlorous acid (HOCl) in neutrophil-free mixtures exposed to beta-D-glucose and glucose oxidase, gamma irradiation, or HOCl, respectively. Our results suggest that TU or DMTU can decrease neutrophil-mediated killing of bacteria by inhibiting O2 metabolite-dependent bactericidal mechanisms.
authors.affiliation: Department of Medicine, Webb-Waring Lung Institute, University of Colorado Medical Center, Denver.
authors.forename: J H
authors.initials: JH
authors.lastname: Jackson
authors.forename: E M
authors.initials: EM
authors.lastname: Berger
authors.forename: J E
authors.initials: JE
authors.lastname: Repine
mesh_terms: D001770:Blood Bactericidal Activity / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D013481:Superoxides; C038983:1,3-dimethylthiourea; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D009113:Muramidase; D013890:Thiourea