Complete primary structure of a human plasma membrane Ca2+ pump.
cDNAs coding for a plasma membrane Ca2+ pump were isolated from a human teratoma library and sequenced. The translated sequence contained 1,220 amino acids with a calculated molecular weight of 134,683. All regions of functional importance known from other ion-transporting ATPases could be identified. The translated sequence also contained, near the carboxyl terminus, the calmodulin-binding domain and two domains which are very rich in glutamic acid and aspartic acid. These two domains resemble calmodulin somewhat and one of them may play a role in the binding of Ca2+. The enzyme also contains domains rich in serine and threonine, one of which has a sequence matching those of good cAMP-dependent protein kinase substrates. The carboxyl-terminal region is important for regulation by calmodulin, proteolysis, and phosphorylation. Near the amino terminus are two domains which are very rich in lysine and glutamic acid, as well as two domains resembling EF hands, one of which also has some resemblance to calmodulin. Comparison of the cloned sequence with peptide sequences from the erythrocyte Ca2+ pump showed that the two proteins have a very high proportion of identical residues but are not 100% identical, indicating that they represent different isozymes.
Verma
A K
AK
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905.
Filoteo
A G
AG
Stanford
D R
DR
Wieben
E D
ED
Penniston
J T
JT
Strehler
E E
EE
Fischer
R
R
Heim
R
R
Vogel
G
G
Mathews
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D011487:Protein Conformation; D013045:Species Specificity; D013724:Teratoma
D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes; D000252:Calcium-Transporting ATPases