Biochemical identification of a putative glutamate receptor in housefly thoracic membranes.
Specific stereoselective binding of [3H]L-glutamate was detected to membranes prepared from housefly thorax to which were added several antiproteases. A single high affinity binding site was detected (KD 0.5 +/- 0.04 microM), but total binding varied from preparation to preparation (5-60 pmoles/mg protein). Specific binding was inhibited by preincubation of the membranes with trypsin, chymotrypsin or protease, or by exposure to 70 degrees C for 5 min. It was also inhibited by several compounds, the most potent being L-glutamate and L-aspartate, followed by L-glutamate diethylester, then D-glutamate, N-methyl-D-aspartate and ibotenate. Quisqualate had little effect, while kainate, proctolin and D-aspartate had none. d-Tubocurarine stimulated [3H]L-glutamate binding. The data suggest that [3H]L-glutamate is binding to an L-glutamate receptor in housefly thoracic muscle membranes.
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D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism; D004175:Diptera / Q000378:metabolism*; D005724:Ganglia / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006358:Hot Temperature; D007700:Kinetics; D009132:Muscles / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013237:Stereoisomerism; D013909:Thorax / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D005971:Glutamates; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D018698:Glutamic Acid; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D014357:Trypsin