title: Preparation of reproducible alkaline phosphatase-antibody conjugates for enzyme immunoassay using a heterobifunctional linking agent.
abstract: Conjugates of alkaline phosphatase (AP) and mouse monoclonal immunoglobulins G (IgG) were prepared by means of the heterobifunctional linker, N-succinimidyl 3-(2-pyridyldithio)-propionate. The efficiency of such conjugates can be improved by optimizing the degree of substitution of IgG and AP. We have determined conditions yielding better performing conjugates than those synthesized by methods described previously. Moreover, the results obtained with the technique presented here are quite reproducible with all four monoclonal antibodies tested.
authors.affiliation: General Biology Department, University of Liège, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Jeanson
authors.forename: J M
authors.initials: JM
authors.lastname: Cloes
authors.forename: M
authors.initials: M
authors.lastname: Bouchet
authors.forename: B
authors.initials: B
authors.lastname: Rentier
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D000911:Antibodies, Monoclonal; D005976:Glutaral; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D010504:Periodic Acid; D013388:Succinimides
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D013388:Succinimides; D010504:Periodic Acid; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate; C009288:metaperiodate; D000469:Alkaline Phosphatase; D005976:Glutaral