[Ecto-ATPases in leukocytes and its modification by drugs].
The ecto-ATPase is located on the external surface of the plasma membrane of intact cells. The enzyme splits exogeneous ATP in the presence of Mg2+ into ADP and inorganic phosphate. This enzyme has been found on a variety of cells of various species in different activities. In leukocytes sulfhydryl groups are essential for the structure and function of the ecto-ATPase. The physiological and biochemical significance of the ecto-ATPase for cells remains unclear. Drugs may influence ecto-ATPase activity and simultaneously various cell functions. Tricyclic antidepressants and phenothiazine tranquilizers in micromolar concentrations inhibit the ecto-ATPase in granulocytes and reduce the phagocytic activity of the cells. The inhibition of the ecto-ATPase of rat thymocytes by organic tin compounds is not connected with a reduced spontaneous migration of the cells in vitro. Newer results indicate, that the ecto-ATPase activity of thymocytes and other cells is changing during maturation and differentiation.
Hehl
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EM
Hennighausen
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Lange
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D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000502:physiology; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology*; D014150:Antipsychotic Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology; D004791:Enzyme Inhibitors; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D051379:Mice; D010640:Phenothiazines; D051381:Rats; D013045:Species Specificity; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000201:enzymology; D013950:Thymus Gland / Q000166:cytology
D000929:Antidepressive Agents, Tricyclic; D014150:Antipsychotic Agents; D004791:Enzyme Inhibitors; D010640:Phenothiazines; D013439:Sulfhydryl Reagents; D000251:Adenosine Triphosphatases; C027201:ectoATPase
Ekto-ATPasen an Leukozyten und ihre Beeinflussung durch Pharmaka.