title: Minimum number of cells required for reconstitution of a foot-and-mouth disease virus-carrier cell culture.
abstract: A serial cell plating experiment has been designed to determine the minimum number of cells, isolated from a culture persistently infected with a virus, required to reconstruct the carrier state. For cell line C1-BHK-Rc1, consisting of BHK-21 cells persistently infected with foot-and-mouth disease virus type C (isolate C-S8c1), more than 10(3) cells derived from one monolayer were needed to reinitiate a stable, FMDV-producing carrier culture. Thus, the FMDV-BHK-21 cell system cannot be explained merely as a sum of its individual components. The results suggest, instead, that the behaviour of C1-BHK-Rc1 cultures is the result of complex interactions acting at the population level. Implications for viral persistence are discussed.
authors.affiliation: Centro de Biología Molecular Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: de la Torre
authors.forename: E
authors.initials: E
authors.lastname: Domingo
mesh_terms: D000818:Animals; D005537:Aphthovirus / Q000254:growth & development*; D001431:Bacteriological Techniques; D002353:Carrier State / Q000382:microbiology; D002452:Cell Count; D002478:Cells, Cultured; D005536:Foot-and-Mouth Disease / Q000382:microbiology