title: [Immobilization of glucose oxidase on silica-based supports].
abstract: Glucose oxidase (beta-D-glucose: oxygen 1-oxidoreductase, EC 1.1.3.4) was covalently coupled to silica-based supports containing aldehyde functional groups. The activity of the immobilized enzyme was about 1000 U/g support. The optimum pH of the catalytic activity was 5.5 for the soluble enzyme and 6.0 for the immobilized enzyme. With glucose as a substrate the Km value of the immobilized enzyme was higher than in case of the soluble enzyme. The immobilized enzyme was found to be more thermostable than the soluble one. The immobilization did not affect the stability of glucose oxidase against the denaturing effect of urea.
authors.forename: M
authors.initials: M
authors.lastname: Abraham
authors.forename: L
authors.initials: L
authors.lastname: Boross
authors.forename: B
authors.initials: B
authors.lastname: Szajani
mesh_terms: D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D012822:Silicon Dioxide; D013379:Substrate Specificity
chemical_list: D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D005949:Glucose Oxidase
vernacular_title: Immobilizatsiia gliukozooksidazy na nositeliakh s silikatnymi osnovami.