title: Increased muscarinic receptor binding in heart membranes by an inhibitor of protein kinase C.
abstract: The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [3H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg2+ reduced MAChR number by 50%. Incubation with polymyxin B, an inhibitor of protein kinase C, blocked the effect of ATP/Mg2+ and increased MAChR number by 74%.
authors.affiliation: Medical College of Georgia, Department of Anatomy, Augusta 30912-2000.
authors.forename: T L
authors.initials: TL
authors.lastname: Creazzo
authors.forename: R W
authors.initials: RW
authors.lastname: Wrenn
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D008274:Magnesium / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism*; D010766:Phosphorylation; D011112:Polymyxin B / Q000494:pharmacology*; D011113:Polymyxins / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D011113:Polymyxins; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D000255:Adenosine Triphosphate; D011493:Protein Kinase C; D008274:Magnesium; D011112:Polymyxin B