title: Inhibition of mouse epidermal 12-lipoxygenase by 2,3,4-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinon e (AA861).
abstract: 2,3,5-Trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone (AA861) strongly inhibited epidermal lipoxygenase activity which was determined by the formation of [14C]12-hydroxy-5,8,10,14-eicosatetraenoic acid by incubating [14C]arachidonic acid with cytosol fraction of epidermal homogenate of CD-1 mice. AA861 failed to inhibit epidermal cyclooxygenase activity. The present results indicate that AA861 is a potent inhibitor of 12-lipoxygenase in mouse epidermis (IC50 1.9 microM).
authors.forename: T
authors.initials: T
authors.lastname: Nakadate
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: E
authors.initials: E
authors.lastname: Aizu
authors.forename: R
authors.initials: R
authors.lastname: Kato
mesh_terms: D000818:Animals; D001091:Arachidonate Lipoxygenases; D016227:Benzoquinones; D001792:Blood Platelets / Q000201:enzymology; D004817:Epidermis / Q000201:enzymology*; D005260:Female; D016859:Lipoxygenase Inhibitors; D051379:Mice; D008815:Mice, Inbred Strains; D011809:Quinones / Q000494:pharmacology*
chemical_list: D016227:Benzoquinones; D016859:Lipoxygenase Inhibitors; D011809:Quinones; C036837:2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone; D001091:Arachidonate Lipoxygenases