Localization by immunoelectron microscopy of somatostatin-containing cells in the gastric mucosa of a teleost fish, Perca fluviatilis.
Somatostatin-immunoreactive cells were localized on semithin and ultrathin sections of Epon-embedded samples of perch gastric mucosa, classically fixed with aldehydes and osmium tetroxide. On semithin sections, somatostatin cells were identified by using the immunoperoxidase method. The ultrastructural localization of somatostatin immunoreactivity was achieved using the colloidal gold method. Cells showing somatostatin immunoreactivity are found to be scattered among the surface mucous cells and the mucous neck cells. Somatostatin appears to be localized in cytoplasmic granules. Somatostatin-containing cells are identified as the type I cells which were described in a previous ultrastructural study. The present report also points out that tissue samples which have been classically processed for ultrastructural study could be in some cases suitable for immunocytochemical investigations.
Noaillac-Depeyre
J
J
D000818:Animals; D003594:Cytoplasmic Granules / Q000032:analysis / Q000648:ultrastructure; D005399:Fishes / Q000378:metabolism*; D005753:Gastric Mucosa / Q000032:analysis / Q000166:cytology* / Q000648:ultrastructure; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D013004:Somatostatin / Q000032:analysis*
D013004:Somatostatin