Proteins expressed by the c-myc oncogene in lymphomas of human and avian origin.
We have prepared antisera against synthetic peptides corresponding to the C-terminal region of the avian and human myc oncogene coding sequences. Immunoprecipitates from avian and human cells show two major proteins which, by the criteria of hybrid-selected translation, transfection, and peptide-blocking assays, are the c-myc protein products. These proteins are phosphorylated nuclear proteins which are tightly bound to the nuclear matrix-lamin and which have a short half-life. Analysis of avian and human lymphoma cell lines containing rearranged c-myc alleles show significant changes in the ratio of the two proteins although only the avian lymphomas have increased quantities of c-myc protein.
Eisenman
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RN
Hann
S R
SR
D000818:Animals; D000906:Antibodies; D002467:Cell Nucleus / Q000648:ultrastructure; D002645:Chickens; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D008223:Lymphoma / Q000235:genetics*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D009857:Oncogenes; D014176:Protein Biosynthesis
D000906:Antibodies; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins