Tumour necrosis factors modulate the affinity state of the leukotriene B4 receptor on human neutrophils.
Pre-incubation of human polymorphonuclear granulocytes with recombinant human tumour necrosis factors (TNF) revealed a time- and dose-dependent reduction of the expression of leukotriene B4-receptor sites. Analysis of the binding data by Scatchard plots showed a shift from a heterologous receptor population (indicating high- and low-affinity subsets) to a homologous population. From the results it is considered that TNF can influence host defence through the modulation of leukotriene B4 receptor affinity.
Brom
J
J
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Arbeitsgruppe für Infektabwehrmechanismen, Ruhr-Universität Bochum, FRG.
Knöller
J
J
Köller
M
M
König
W
W
D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D011971:Receptors, Immunologic / Q000032:analysis / Q000378:metabolism*; D018102:Receptors, Leukotriene B4; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D011971:Receptors, Immunologic; D018102:Receptors, Leukotriene B4; D014409:Tumor Necrosis Factor-alpha