Inhibition of the import of mitochondrial proteins by RNase.
RNase treatment of a cell-free translation system prevents transport of mitochondrial precursor proteins from that system into isolated yeast mitochondria. This inhibition depends on the presence of ribosomes in the reticulocyte lysate; if they are cleared by centrifugation, RNase treatment does not specifically inhibit protein uptake by mitochondria. Since protein import can occur in the absence of polyribosomes, RNase treatment does not degrade a structure essential for this process. Rather, the inhibition may be an effect of degraded ribosomes.
Burns
D
D
Lewin
A
A
D001693:Biological Transport, Active / Q000187:drug effects; D002474:Cell-Free System; D002950:Citrate (si)-Synthase / Q000096:biosynthesis; D004792:Enzyme Precursors / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D014176:Protein Biosynthesis; D006180:Proton-Translocating ATPases / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000648:ultrastructure
D004792:Enzyme Precursors; D012333:RNA, Messenger; D002950:Citrate (si)-Synthase; D012260:Ribonucleases; D006180:Proton-Translocating ATPases