Single dilution ELISA for detection of serum antibody to foot-and-mouth disease virus in cattle.
A single dilution blocking ELISA was developed and evaluated for measuring serum antibody to foot-and-mouth disease virus (FMDV). Basic parameters of the assay were established and a positive-negative threshold determined from testing 176 specific antibody negative sera from Australian cattle. Sera collected from immunised animals in Thailand were tested by ELISA and virus-neutralisation (VN) tests and the results compared. A positive correlation between ELISA and VN titres was recorded for each of the 3 FMDV serotypes endemic in Thailand, with the overall correlation coefficient being r = 0.8990. A positive correlation for each of the serotypes was also found between ELISA titre and the degree of blocking (percentage inhibition) of each test serum at a dilution of 1:16, with the overall correlation being r = 0.8704. This simplified ELISA was sensitive, specific and gave reproducible results, and had the potential to test quickly and efficiently a considerable number of sera.
Westbury
H A
HA
Australian Animal Health Laboratory, Geelong, Vic.
Chamnanpood
P
P
Tangchaitrong
S
S
Doughty
W J
WJ
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D005537:Aphthovirus / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D012016:Reference Values; D013785:Thailand
D000914:Antibodies, Viral