Purification and characterization of a single form of cytochrome P-450 from the spiny crab Maja crispata.
Microsomes from Maja crispata hepatopancreas contain all the components of the functional mixed function oxidase system: cytochrome P-450 (0.47 nmol/mg), the activity of NADPH cytochrome c reductase (12.25 nmol/mg/min) and benzo[a]pyrene monooxygenase activity (6.58 pmol/mg/min). Solubilization of hepatopancreas microsomes with sodium cholate, and affinity chromatography on omega-amino-n-octyl Sepharose 4B, gave a single cytochrome P-450 peak eluting with 0.2% Emulgen 913. DEAE cellulose chromatography of this cytochrome peak gave rise to a single haemoprotein peak, with apparent monomer Mr = 53,500, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis.
Batel
R
R
Bihari
N
N
Zahn
R K
RK
D000818:Animals; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D003386:Brachyura / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology
D003577:Cytochrome P-450 Enzyme System; D001579:Benzopyrene Hydroxylase; D009251:NADPH-Ferrihemoprotein Reductase