title: S6 kinase in quiescent Swiss mouse 3T3 cells is activated by phosphorylation in response to serum treatment.
abstract: To investigate the role of phosphorylation in the activation of S6 kinase, the enzyme was isolated from 32P-labeled Swiss mouse 3T3 cells before and after stimulation with serum. The kinase activity was followed through several purification steps, and a radioactive protein of Mr 70,000 was obtained from the stimulated cells. This band was not detected in resting cells. The Mr 70,000 protein exhibited the same size upon NaDodSO4/PAGE as the homogeneous kinase, and it comigrated with the in vitro autophosphorylated form of the enzyme. Treatment of the in vivo-labeled material with phosphatase 2A led to a loss of kinase activity concomitant with a release of 32Pi from the Mr 70,000 protein. The partially dephosphorylated protein migrated faster during PAGE, displaying distinct species of Mr 69,000 and 68,000. Most importantly, phospho amino acid analysis of the labeled S6 kinase showed only phosphoserine and phosphothreonine. These results argue that the S6 kinase is phosphorylated at multiple sites in vivo and that it is activated by serine/threonine phosphorylation.
authors.affiliation: Friedrich Miescher-Institut, Basel, Switzerland.
authors.forename: L M
authors.initials: LM
authors.lastname: Ballou
authors.forename: M
authors.initials: M
authors.lastname: Siegmann
authors.forename: G
authors.initials: G
authors.lastname: Thomas
mesh_terms: D000818:Animals; D001665:Binding Sites; D001769:Blood; D051379:Mice; D008970:Molecular Weight; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D054648:Protein Phosphatase 2; D019893:Ribosomal Protein S6 Kinases
chemical_list: D011494:Protein Kinases; D019893:Ribosomal Protein S6 Kinases; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2