title: Displacement of 3H-EKC binding by opioids in rat kidney: a correlate to diuretic activity.
abstract: Multiple opioid binding sites have been documented in brain tissue. In this study we report on the presence of binding sites for the opioid ethylketocyclazocine (EKC) in a membrane fraction of rat kidney. Binding appeared to be selective in that opioids varied markedly in their capacities to displace 3H-EKC. Correlating with the capacity of an opioid to displace 3H-EKC was the ability to produce diuresis. Although our studies cannot assign a particular physiological or pharmacological role for the renal EKC binding sites, binding studies of this nature may, nonetheless, be a means by which diuretic activity of opioids can be predicted.
authors.forename: G R
authors.initials: GR
authors.lastname: Slizgi
authors.forename: J H
authors.initials: JH
authors.lastname: Ludens
mesh_terms: D000701:Analgesics, Opioid / Q000378:metabolism*; D000818:Animals; D003496:Cyclazocine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016314:Ethylketocyclazocine; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000378:metabolism*; D014316:Tritium
chemical_list: D000701:Analgesics, Opioid; D004232:Diuretics; D011957:Receptors, Opioid; D014316:Tritium; D016314:Ethylketocyclazocine; D003496:Cyclazocine