The putative trans-activator in the MAgag region of Rous sarcoma virus is not required for cell transformation.
A stop codon created by oligonucleotide-directed mutagenesis in the proposed transcriptional trans-activator of Rous sarcoma virus (S. Broome and W. Gilbert, Cell 40:537-546, 1985) which truncated the trans-activator by half did not alter the transforming activity or the replication of the virus in primary chicken embryo fibroblasts. This result proves that this trans-activator is not essential for transformation of primary cells by Rous sarcoma virus.
Dutta
A
A
Rockefeller University, New York, New York 10021-6399.
Dorai
T
T
Hanafusa
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005347:Fibroblasts; D015683:Gene Products, gag; D015696:Gene Products, tat; D008969:Molecular Sequence Data; D009154:Mutation; D011533:Proviruses / Q000235:genetics* / Q000502:physiology; D012367:RNA, Viral / Q000032:analysis; D012191:Retroviridae Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection; D014779:Virus Replication
D004279:DNA, Viral; D015683:Gene Products, gag; D015696:Gene Products, tat; D012367:RNA, Viral; D012191:Retroviridae Proteins; D014157:Transcription Factors