Effect of K+ channel blockers on anti-immunoglobulin-induced murine B cell proliferation.
Ion channels play an important role in the transmission of signals across cell membrane in a variety of cell types. Recent studies indicate that normal murine splenic B lymphocytes express voltage gated K+ channels. In the present study, we examined the influence of K+ channel blockers 4 aminopyridine (4 AP) and tetraethyl ammonium, and Ca++ activated K+ channel blocker quinine on anti-immunoglobulin (anti-Ig)-induced B cell proliferation. The results show that ion channel blockers inhibited DNA synthesis in B cells induced by anti-Ig. 4 AP also inhibited protein synthesis but had no effect on the expression of surface immunoglobulin. 4 AP exerted its maximum suppressive effects on DNA synthesis when added within the first 24 hours after the onset of culture with anti-Ig. The inhibition of DNA synthesis by 4 AP was reversible upon its removal from culture. These studies show a role of K+ channels in B cell DNA and protein synthesis.
Gollapudi
S V
SV
Department of Medicine, University of California, Irvine 92717.
Vayuvegula
B S
BS
Thadepalli
H
H
Gupta
S
S
D015761:4-Aminopyridine; D000631:Aminopyridines / Q000494:pharmacology*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015221:Potassium Channels / Q000187:drug effects*; D014176:Protein Biosynthesis; D011803:Quinine / Q000494:pharmacology*; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology*
D000631:Aminopyridines; D000888:Antibodies, Anti-Idiotypic; D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D019789:Tetraethylammonium; D004247:DNA; D011803:Quinine; D015761:4-Aminopyridine