title: Neurotensin binds with high affinity to small cell lung cancer cells.
abstract: Several small cell lung cancer cell lines bound 125I-neurotensin with high affinity. Radiolabeled neurotensin bound with high affinity (Kd = 4 nM) to a single class of sites (2300/cell) using cell line NCI-H209. Binding was time dependent and reversible. Pharmacology studies indicated that the C-terminal of neurotensin was important for the high affinity binding activity. Because high concentrations of neurotensin and its receptors are associated with small cell lung cancer, neurotensin may function as a regulatory peptide in this disease.
authors.affiliation: Department of Biochemistry, George Washington University School of Medicine and Health Sciences, Washington, DC 20037.
authors.forename: A E
authors.initials: AE
authors.lastname: Allen
authors.forename: D N
authors.initials: DN
authors.lastname: Carney
authors.forename: T W
authors.initials: TW
authors.lastname: Moody
mesh_terms: D001667:Binding, Competitive; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D009496:Neurotensin / Q000378:metabolism*; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter; D009496:Neurotensin