A combination of palytoxin with 1-oleoyl-2-acetyl-glycerol (OAG) or insulin or interleukin-1 synergistically stimulates arachidonic acid metabolism, but combinations of 12-O-tetradecanoylphorbol-13-acetate (TPA)-type tumor promoters with OAG do not.
The combination of palytoxin and 1-oleoyl-2-acetyl-glycerol (OAG) synergistically stimulates production of 6-keto-PGF1 alpha and PGF2 alpha by rat liver cells (the C-9 cell line). In contrast, the combination of 12-O-tetradecanoylphorbol-13-acetate (TPA)-type tumor promoters (TPA, dihydroteleocidin B, aplysiatoxin, phorbol-12,13-didecanoate) and OAG does not. Production of 6-keto-PGF1 alpha by palytoxin added with recombinant murine interleukin-1 (IL-1) or with insulin is also greater than the sum of the two effects taken independently. Palytoxin and OAG individually stimulate the release of radiolabeled compounds from the rat liver cells pre-labeled with [3H]arachidonic acid and also act synergistically to release labeled metabolites. After separation by h.p.l.c., these materials co-chromatograph with authentic 6-keto-PGF1 alpha and arachidonic acid. The synergistic stimulation by palytoxin and OAG is biphasic; a rapid synergistic production of 6-keto-PGF1 alpha or release of radiolabel from [3H]arachidonic acid prelabeled cells is followed, after approximately 2-4 h, by a prolonged synergistic response.
Levine
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Xiao
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Fujiki
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D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000178:Acrylamides; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D003064:Cnidarian Venoms / Q000494:pharmacology*; D004075:Diglycerides / Q000494:pharmacology*; D015237:Dinoprost; D004357:Drug Synergism; D005989:Glycerides / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver; D010704:Phorbols / Q000494:pharmacology*; D011460:Prostaglandins F / Q000096:biosynthesis; D051381:Rats; D013329:Structure-Activity Relationship; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D000095702:Polyether Toxins
D015121:6-Ketoprostaglandin F1 alpha; D000178:Acrylamides; D016718:Arachidonic Acid; D001095:Arachidonic Acids; D002273:Carcinogens; D003064:Cnidarian Venoms; D004075:Diglycerides; D015237:Dinoprost; D005989:Glycerides; D007328:Insulin; D007375:Interleukin-1; D010704:Phorbols; D011460:Prostaglandins F; D013755:Tetradecanoylphorbol Acetate; C038237:1-oleoyl-2-acetylglycerol; C010272:palytoxin; D000095702:Polyether Toxins