title: Isolation of carcinogen-induced diploid rat hepatocytes by centrifugal elutriation.
abstract: The majority of hepatocytes isolated from rats treated with carcinogens (diethylnitrosamine plus 2-acetylaminofluorene) were found to be diploid, whereas most of the hepatocytes from normal rats are tetraploid. The carcinogen-induced diploid hepatocytes were only one-half the size (protein content) of the tetraploid hepatocytes, and could therefore be separated from the latter by centrifugal elutriation. The elutriation technique thus makes it possible to isolate a relatively pure fraction of carcinogen-induced cells. The diploid cells had the same liver-specific enzymatic and functional properties as the tetraploid cells and were thus undoubtedly of hepatocytic origin.
authors.forename: P E
authors.initials: PE
authors.lastname: Schwarze
authors.forename: E O
authors.initials: EO
authors.lastname: Pettersen
authors.forename: H
authors.initials: H
authors.lastname: Tolleshaug
authors.forename: P O
authors.initials: PO
authors.lastname: Seglen
mesh_terms: D000818:Animals; D002469:Cell Separation; D004247:DNA / Q000032:analysis; D004052:Diethylnitrosamine / Q000494:pharmacology*; D004171:Diploidy; D005434:Flow Cytometry; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D004052:Diethylnitrosamine; D004247:DNA; D005723:gamma-Glutamyltransferase