Transient transcriptional inhibition of the transferrin gene by cyclic AMP.
Transferrin mRNA content and gene transcription rate were measured in the liver of rats submitted to iron overload or depletion, castration, treatment with sexual steroid hormones, glucagon and cyclic AMP. The influence of puberty in males and females and of pregnancy was also analysed. Glucagon and cyclic AMP reduced mRNA level by about 50% at the 12th hour of treatment and transferrin gene transcription by as much as 95% at the 30th minute of drug infusion, with a secondary increase of the transcription rate for a protracted treatment. None of the other hormones tested had any detectable effect on transferrin gene expression, the same being true for iron overload or depletion.
Tuil
D
D
Vaulont
S
S
Levin
M J
MJ
Munnich
A
A
Moguilewsky
M
M
Bouton
M M
MM
Brissot
P
P
Dreyfus
J C
JC
Kahn
A
A
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002369:Castration; D000242:Cyclic AMP / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005934:Glucagon / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D008099:Liver / Q000032:analysis; D008297:Male; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D014168:Transferrin / Q000235:genetics*
D012333:RNA, Messenger; D014168:Transferrin; D011061:Poly A; D013739:Testosterone; D004958:Estradiol; D003994:Bucladesine; D005934:Glucagon; D000242:Cyclic AMP; D007501:Iron