A novel EBNA-1 titration method and putative anti-EBNA-1 protein.
A novel and rapid EBNA-1 titration method has been developed which uses immunoprecipitation of specific DNA-protein complexes with EBNA-1-positive serum. The method is more sensitive than the conventional immunofluorescence method and has potential value as a diagnostic reagent for clinical laboratories. TPA induction of putative anti-EBNA-1 protein of cellular origin is discussed, which may play a key role for the shift from latent to lytic replication of EBV.
Nonoyama
M
M
Department of Virology, Showa-Teikyo International Research Center, St. Petersburg, Florida 33716.
Wen
L T
LT
Tabata
T
T
Tanaka
A
A
D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D001667:Binding, Competitive; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism; D019309:Epstein-Barr Virus Nuclear Antigens; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000648:ultrastructure; D011233:Precipitin Tests; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D013755:Tetradecanoylphorbol Acetate