An enzyme-linked immunosorbent assay for the detection of vesicular stomatitis virus antigen.
An indirect, sandwich enzyme-linked immunosorbent assay (ELISA) has been developed for the laboratory diagnosis of vesicular stomatitis (VS). The assay which uses rabbit and guinea-pig antisera to the purified glycoprotein antigens of Serotypes New Jersey (VSV-NJ) and Indiana (VSV-IND-1), has both high sensitivity and specificity, but has lower reactivity for the two other Indiana subtypes VSV-IND-2 and VSV-IND-3.
Ferris
N P
NP
Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, Gt. Britain.
Donaldson
A I
AI
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006168:Guinea Pigs; D011817:Rabbits; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology / Q000302:isolation & purification*; D018116:Vesiculovirus
D000956:Antigens, Viral