Isolation of carcinogen-induced diploid rat hepatocytes by centrifugal elutriation.
The majority of hepatocytes isolated from rats treated with carcinogens (diethylnitrosamine plus 2-acetylaminofluorene) were found to be diploid, whereas most of the hepatocytes from normal rats are tetraploid. The carcinogen-induced diploid hepatocytes were only one-half the size (protein content) of the tetraploid hepatocytes, and could therefore be separated from the latter by centrifugal elutriation. The elutriation technique thus makes it possible to isolate a relatively pure fraction of carcinogen-induced cells. The diploid cells had the same liver-specific enzymatic and functional properties as the tetraploid cells and were thus undoubtedly of hepatocytic origin.
Schwarze
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Pettersen
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Tolleshaug
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Seglen
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D000818:Animals; D002469:Cell Separation; D004247:DNA / Q000032:analysis; D004052:Diethylnitrosamine / Q000494:pharmacology*; D004171:Diploidy; D005434:Flow Cytometry; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D004052:Diethylnitrosamine; D004247:DNA; D005723:gamma-Glutamyltransferase