Structural changes in DNA of human breast carcinoma.
Rearrangement and loss of genetic material in DNA of human ductal breast carcinoma was analyzed by restriction enzyme digestion, agarose gel electrophoresis and Southern blot hybridization using 32P labeled c-Ha-ras-1 and multilocus, minisatellite 15.1.11.4 as a probe. In several cases an allelic loss of c-Ha-ras-1 locus in heterozygous patients were noticed. Rearrangement of the genetic material in neoplastic tissues was also observed after hybridization with a minisatellite probe. These results provide evidence that loss and redistribution of the genetic material does occur in human ductal breast carcinoma.
Mackiewicz
M
M
Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
Choina
B
B
Kadlof
A
A
Szala
S
S
D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000235:genetics*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics*; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D015321:Gene Rearrangement; D006801:Humans
D015342:DNA Probes; D004273:DNA, Neoplasm