Isolation and in vitro translation of Onchocerca volvulus mRNA.
Adult Onchocerca volvulus recovered for excised nodules by dissection or treatment with collagenase have been used as a source of RNA for in vitro translation experiments. RNA was purified using either the hot phenol/SDS procedure or the guanidine isothiocyanate protocol. Immunoprecipitation experiments performed on in vitro products demonstrate a marked heterogeneity in responses by individed human infection sera. Further immunoprecipitation experiments demonstrate cross reactivity between O. volvulus and other filarial nematodes.
Connor
V M
VM
Department of Pathology, University of Cambridge, U.K.
Braun
G
G
Engelbrecht
F
F
Cesbron
J Y
JY
Downham
M J
MJ
Whitworth
J
J
McMahon
J E
JE
Taylor
D W
DW
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D003012:Microbial Collagenase; D009854:Onchocerca / Q000235:genetics* / Q000276:immunology; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*
D000947:Antigens, Helminth; D007106:Immune Sera; D012333:RNA, Messenger; D003012:Microbial Collagenase