Combined herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid leads to oncogenic transformation.
Tumorigenic transformation was produced by combining herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid (each is associated with cervical carcinoma). Recombinant human papillomavirus type 16 and 18 deoxyribonucleic acid each containing a head-to-tail dimer of the full-length human papillomavirus respective genomes, induced morphologic transformation in an immortalized cell line (AE), obtained by transfection of Syrian hamster embryo cells with immortalizing sequences of herpes simplex virus type 2 deoxyribonucleic acid. These transformed cells were tumorigenic in nude mice. Deoxyribonucleic acid of both human papillomaviruses induced morphologically transformed foci in Syrian hamster embryo cell cultures, but these transformed cells were not tumorigenic. Southern blot analyses revealed transfected deoxyribonucleic acid in the human papillomavirus type 16 transformed cells, whereas viral deoxyribonucleic acid was not evident in the human papillomavirus type 18 transformed cells. These events suggest a "hit and run" oncogenesis.
Iwasaka
T
T
Department of Obstetrics and Gynecology, Saga Medical School, Japan.
Yokoyama
M
M
Hayashi
Y
Y
Sugimori
H
H
D000818:Animals; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000032:analysis; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics / Q000502:physiology*; D006561:Herpes Simplex / Q000145:classification / Q000235:genetics*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*
D004279:DNA, Viral