Effect in vitro of bovine viral diarrhea virus on bovine embryos with the zona pellucida intact, damaged and removed.
The in vitro effect of bovine viral diarrhea virus (BVDV) on the survival of day 7 to day 7.5 bovine embryos collected from superovulated donors was studied. Fifty-four experimental embryos with the zona pellucida (ZP) intact, damaged or removed were exposed to 1 X 10(4) TCD50/ml of the NADL cytopathic strain of BVDC at 37 degrees C for 24 hrs and compared to 36 control embryos that were cultured for 24 hr. Seven embryos with the ZP-removed were similarly exposed for 48 hrs and compared to five control embryos. The overall survival rate was 68% for embryos exposed to BVDV for 24 hrs and 77% for embryos not exposed (P greater than 0.05). Extended exposure of the embryos with the ZP removed to virus for 48 hrs did not affect their survival rate compared to controls. Damage to the ZP by cracking or total removal of the ZP by micromanipulation or acidic Tyrode's solution had no effect on subsequent embryonic survival in the presence of BVDV. It was concluded that exposure to BVDV in vitro is not cytopathic for morula and blastocyst stage bovine embryos over a 48 hr period, even when they are not protected by the ZP.
Bielanski
A
A
Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario.
Hare
W C
WC
D000818:Animals; D001755:Blastocyst / Q000382:microbiology* / Q000502:physiology; D002417:Cattle / Q000196:embryology*; D002970:Cleavage Stage, Ovum / Q000382:microbiology*; D046508:Culture Techniques; D001908:Diarrhea Viruses, Bovine Viral / Q000502:physiology*; D005260:Female; D009028:Morula / Q000382:microbiology* / Q000502:physiology; D010063:Ovum / Q000502:physiology*; D010576:Pestivirus / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*