A unique selenoprotein isolated from yellowfin tuna (Thunnus albacares) liver.
A selenoprotein, with an approximate molecular weight of 2000, was isolated from yellowfin tuna (Thunnus albacares) liver. The selenium (Se) content of this selenoprotein fraction represented greater than 50% of the Se in the original liver extract. Most of the unrecovered Se was left in the pellet following homogenization. Although the protein was very sensitive to oxidizing conditions, it remained stable in the presence of reducing agents such as glutathione and dithiothreitol under a nitrogen atmosphere. After preparative isoelectric focusing of the purified selenoprotein, selenium was detected in three distinct bands, with the predominant band occurring at pH 6.2.
Braddon-Galloway
S
S
Sumpter
C R
CR
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005399:Fishes / Q000378:metabolism*; D007525:Isoelectric Focusing; D008099:Liver / Q000032:analysis; D011506:Proteins; D012643:Selenium / Q000032:analysis; D051140:Selenoproteins; D014413:Tuna / Q000378:metabolism*
D000596:Amino Acids; D002352:Carrier Proteins; D011506:Proteins; D051140:Selenoproteins; D012643:Selenium