title: Oxygen-dependent killing of Staphylococcus aureus by human neutrophils.
abstract: Luminol-dependent chemiluminescence was used as a monitor of reactive oxidant generation during phagocytosis of Staphylococcus aureus by human neutrophils. Reactive oxidants play a crucial role in the killing of this organism because: (a) S. aureus was killed most rapidly when the rate of increase of chemiluminescence was greatest; (b) neutrophils which had been activated to generate reactive oxidants by re-aeration of anaerobic suspensions killed this bacterium more efficiently than control suspensions; and (c) neutrophils from a patient with chronic granulomatous disease could neither generate reactive oxidants nor kill S. aureus.
authors.affiliation: Department of Biochemistry, University of Liverpool, UK.
authors.forename: S W
authors.initials: SW
authors.lastname: Edwards
authors.forename: J E
authors.initials: JE
authors.lastname: Say
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001770:Blood Bactericidal Activity; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D010100:Oxygen