[A decrease in the stimulated level of free intracellular calcium in thrombocytes during refractoriness].
The concentration of free intracellular calcium (Ca.f) was determined in ADP-stimulated platelets. Free calcium was measured by the multichannel method using fluorescent probe indo 1. In the presence of extracellular calcium, ADP induced a rapid reversible increase in Ca.f followed by reversible platelet aggregation. The second addition of ADP also induced increase in Ca.f and platelet aggregation, which were of lesser magnitude (platelet refractoriness). Without extracellular calcium, ADP also produced an increase in Ca.f. In this case the second addition of ADP also induced considerably less increase in Ca.f.
Pozin
E Ia
EIa
Popov
E G
EG
Gavrilov
I Iu
IIu
Gabbasov
Z A
ZA
Krotov
S N
SN
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D000242:Cyclic AMP / Q000097:blood; D003593:Cytoplasm / Q000378:metabolism; D005456:Fluorescent Dyes; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D013268:Stimulation, Chemical
D005456:Fluorescent Dyes; D000244:Adenosine Diphosphate; D000242:Cyclic AMP; D002118:Calcium
Snizhenie stimulirovannogo urovnia svobodnogo vnutrikletochnogo kal'tsiia v trombotsitakh pri refrakternosti.