title: Contrasting in vitro lymphocyte chemotactic activity of the hydroxyl enantiomers of 12-hydroxy-5,8,10,14-eicosatetraenoic acid.
abstract: 1. The chemotactic activity of 12(R)-hydroxy-5,8,10,14-eicosatetraenoic acid (12(R)-HETE), 12(S)-HETE and leukotriene B4 (LTB4) for human mixed peripheral blood lymphocytes has been assessed in a 48-well microchemotaxis assay. Responses to the standard lymphocyte chemoattractants, zymosan-activated plasma, casein and N-formyl-methionyl-leucyl-phenylalanine (fMLP) were also measured. 2. 12(R)-HETE was shown to be chemotactic for lymphocytes over the range 5 x 10(-7) to 5 x 10(-5) M. In contrast, negligible chemotactic responses to 12(S)-HETE were obtained. 3. LTB4 was 200 times more potent than 12(R)-HETE as a lymphocyte chemoattractant, although maximal responses to the two agonists were similar. 4. 12(R)-HETE and LTB4, which are present in extracts of samples from the skin lesions of psoriasis, may be, at least in part, responsible for the lymphocyte infiltrate which is a characteristic feature of this disease.
authors.affiliation: Institute of Dermatology, St. Thomas's Hospital, London.
authors.forename: K B
authors.initials: KB
authors.lastname: Bacon
authors.forename: R D
authors.initials: RD
authors.lastname: Camp
authors.forename: F M
authors.initials: FM
authors.lastname: Cunningham
authors.forename: P M
authors.initials: PM
authors.lastname: Woollard
mesh_terms: D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000494:pharmacology*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D007975:Leukotriene B4 / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects*; D009000:Monocytes / Q000187:drug effects; D013237:Stereoisomerism
chemical_list: D002630:Chemotactic Factors; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4