Limited tryptic proteolysis of the benzodiazepine binding proteins in different species reveals structural homologies.
Peptide mapping can be used to elucidate further the structural similarities of the benzodiazepine binding proteins in different vertebrate species. Crude synaptic membrane preparations were photoaffinity-labeled with [3H]flunitrazepam and subsequently degraded with various concentrations of trypsin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by fluorography allowed a comparison of the molecular weights of photolabeled peptides in different species. Tryptic degradation led to a common peptide of 40K in all species investigated, a finding indicating that the benzodiazepine binding proteins are structurally homologous in higher bony fishes and tetrapods.
Friedl
W
W
Institut für Humangenetik, Universität Bonn, F.R.G.
Lentes
K U
KU
Schmitz
E
E
Propping
P
P
Hebebrand
J
J
D000345:Affinity Labels; D000489:Alligators and Crocodiles; D000818:Animals; D002417:Cattle; D004372:Ducks; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005445:Flunitrazepam / Q000378:metabolism; D006168:Guinea Pigs; D007076:Iguanas; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism*; D010777:Photochemistry; D011898:Ranidae; D011963:Receptors, GABA-A / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D012911:Snakes; D013045:Species Specificity; D013552:Swine; D013570:Synaptic Membranes / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*
D000345:Affinity Labels; D010446:Peptide Fragments; D011963:Receptors, GABA-A; D005445:Flunitrazepam; D014357:Trypsin