title: Localization of plasmid loci necessary for the entry of Shigella flexneri into HeLa cells, and characterization of one locus encoding four immunogenic polypeptides.
abstract: We have previously cloned a 44 kb fragment from the virulence plasmid of Shigella flexneri serotype 5 strain M90T which is capable of restoring invasiveness to an avirulent, plasmidless mutant. This report presents a genetic and physical analysis of Tn5 mutations in recombinant clone pHS4108. Tn5 mutagenesis allowed identification of at least five regions implicated in the entry phenotype. These regions were located on a 20 kb portion of pHS4108. Expression of the insertion mutants was studied by immunoblots using the serum of a convalescent monkey infected by S. flexneri 2a, which recognized four plasmid-associated polypeptides. We propose that the four immunogenic polypeptides, a, b, c, and d, are encoded an operon.
authors.affiliation: Service des Entérobactéries, Unité INSERM 199, Institut Pasteur, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Baudry
authors.forename: A T
authors.initials: AT
authors.lastname: Maurelli
authors.forename: P
authors.initials: P
authors.lastname: Clerc
authors.forename: J C
authors.initials: JC
authors.lastname: Sadoff
authors.forename: P J
authors.initials: PJ
authors.lastname: Sansonetti
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D010455:Peptides / Q000235:genetics*; D010957:Plasmids; D014176:Protein Biosynthesis; D012763:Shigella flexneri / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D004251:DNA Transposable Elements; D010455:Peptides