title: Atrial natriuretic peptide receptors are present in brown adipose tissue.
abstract: Binding activities and metabolic effects of atrial natriuretic peptide (ANP) were examined in rat brown fat. ANP binding sites were identified in thin sections of brown adipose. Binding of [125I]-ANP to brown fat membranes was specific, saturable and time-dependent. A single class of high affinity sites (Kd, 1.7 nM; Bmax, 226 fmol/mg protein) was present. ANP increased cGMP synthesis in isolated cells (EC50, 2.5 x 10(-9) M), but did not alter epinephrine-stimulated glycerol production. The results demonstrate the presence of specific ANP receptors in brown fat, activation of which increases cGMP formation; however, since neither ANP nor cGMP affect lipolysis, the biological importance of these receptors remains unknown.
authors.affiliation: Laboratory of Molecular Endocrinology, McGill University, Royal Victoria Hospital, Montreal, QC, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Okamura
authors.forename: P A
authors.initials: PA
authors.lastname: Kelly
authors.forename: J G
authors.initials: JG
authors.lastname: Chabot
authors.forename: G
authors.initials: G
authors.lastname: Morel
authors.forename: M
authors.initials: M
authors.lastname: Belles-Isles
authors.forename: S
authors.initials: S
authors.lastname: Heisler
mesh_terms: D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D006152:Cyclic GMP / Q000096:biosynthesis; D004837:Epinephrine / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D007700:Kinetics; D008066:Lipolysis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP; D005990:Glycerol; D004837:Epinephrine