title: Biochemical properties of pertussis toxin.
abstract: Pertussis toxin is an exotoxin produced by the organism Bordetella pertussis. The toxin binds to receptors on the eukaryotic cell surface. After introduction into the eukaryotic cell, the toxin is activated by ATP and subsequently ADP-ribosylates a family of GTP-binding regulatory proteins interrupting signal transduction within the cell. We have examined the location of several critical sites on the toxin molecule. These sites include the receptor binding site and the ATP binding site. The B oligomer of the toxin was found to contain at least two sites capable of binding glycoproteins suggesting that the B oligomer may have more than one eukaryotic cell receptor binding site. ATP was also shown to bind to a site on the B oligomer. These results indicate that the B oligomer contains several sites necessary for toxin action.
authors.affiliation: Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892.
authors.forename: D L
authors.initials: DL
authors.lastname: Burns
authors.forename: S Z
authors.initials: SZ
authors.lastname: Hausman
authors.forename: M H
authors.initials: MH
authors.lastname: Witvliet
authors.forename: M J
authors.initials: MJ
authors.lastname: Brennan
authors.forename: J T
authors.initials: JT
authors.lastname: Poolman
authors.forename: C R
authors.initials: CR
authors.lastname: Manclark
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D019204:GTP-Binding Proteins / Q000378:metabolism; D015394:Molecular Structure; D037342:Pertussis Toxin; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology
chemical_list: D011956:Receptors, Cell Surface; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D000255:Adenosine Triphosphate; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins