title: Inhibition of catechol estrogen formation in rat liver microsomes by hormonal steroids and related compounds.
abstract: The inhibitory action of a number of different hormonal steroids and related compounds on the 2-hydroxylation of estradiol by male rat liver microsomes was examined by a radiometric assay. Progesterone, Diethylstilbestrol, testosterone and 4-androstenedione were found to be the most potent of the compounds tested but inhibition was also observed with other steroids and a group of androgen analogs which are aromatization inhibitors. The kinetic constant Ki for those steroids which gave linear double reciprocal plots when added to [2-3H]estradiol was determined and the products from [14C]estradiol in the presence of the inhibitors were examined by TLC and autoradiography. The addition of steroids with a 17-hydroxyl group such as testosterone or dihydroequilin resulted in the formation of mainly 2-hydroxyestradiol with smaller amounts of other metabolites while those with a reducible ketonic group such as progesterone, 4-androstenedione, equilin or equilenin gave rise to considerable amounts of estrone in addition to the catechol estrogens. Further purification of the liver microsomes did not alter this effect. The possible role of progesterone and the catechol estrogens in the control of estrogen hydroxylation in liver as well as other aspects of steroid interaction are discussed.
authors.affiliation: Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
authors.forename: J A
authors.initials: JA
authors.lastname: Quail
authors.forename: A M
authors.initials: AM
authors.lastname: Newcombe
authors.forename: P H
authors.initials: PH
authors.lastname: Jellinck
mesh_terms: D000728:Androgens / Q000494:pharmacology; D000735:Androstenedione / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D004054:Diethylstilbestrol / Q000494:pharmacology; D004855:Equilenin / Q000494:pharmacology; D004857:Equilin / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism; D002393:Estrogens, Catechol / Q000096:biosynthesis*; D006728:Hormones / Q000494:pharmacology*; D006900:Hydroxylation; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000494:pharmacology
chemical_list: D000728:Androgens; D002393:Estrogens, Catechol; D006728:Hormones; D004857:Equilin; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D004054:Diethylstilbestrol; C001390:2-hydroxyestradiol; D004855:Equilenin