Reversed phase high performance liquid chromatography of adrenocorticotropin 1-39 and its fragments in the native and oxidized forms.
This paper reports HPLC separations of human ACTH 1-39 and its fragments (ACTH 1-10, 4-10, 11-24) making use of original gradient systems. Both H2O2 or chloramine T were demonstrated to oxidize the Met 4 present in ACTH 1-39, 1-10, and 4-10; the oxidized forms were HPLC separated from the corresponding native polypeptides, indicating that this method is suitable for the identification in biological fluid of ACTH, its fragment and their methionine-sulphoxide derivatives with possible relevance to the problem of ageing and inactivation of active polypeptide.
Antonini
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Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Italy.
Spoto
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D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008715:Methionine / Q000031:analogs & derivatives / Q000032:analysis; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D010902:Pituitary Gland / Q000032:analysis
D010446:Peptide Fragments; D010455:Peptides; D000324:Adrenocorticotropic Hormone; D008715:Methionine; C013111:methionine sulfoxide