title: [Phosphorylation of acetyl-CoA-carboxylase in the chicken liver during intensification of lipogenesis and treatment with nicotinic acid].
abstract: Sites of cAMP and ATP binding which regulate acetyl-CoA-carboxylase phosphorylation rate characterized under conditions of lipogenesis intensification and nicotinic acid action on this enzyme 1500 fold purified and containing proteinkinase activity. The acetyl-CoA-carboxylase preparation contains only one type of the cAMP binding sites which possess higher capacity under the action of nicotinic acid in vivo. A decrease of the cAMP binding under the conditions of lipogenesis intensification is induced by diminution of the cAMP binding site capacity without changing the binding constant value. It is established that [gamma-32P]ATP is incorporated in enzyme with Km value equal for two states under study. It this case the [gamma-32P]ATP incorporation rate is much higher for acetyl-CoA-carboxylase produced from chicken liver under the action of nicotinic acid.
authors.forename: L N
authors.initials: LN
authors.lastname: Khustochka
authors.forename: A G
authors.initials: AG
authors.lastname: Khalmuradov
authors.forename: A I
authors.initials: AI
authors.lastname: Fomenko
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D000242:Cyclic AMP / Q000378:metabolism; D007700:Kinetics; D008025:Ligases / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D009525:Niacin / Q000494:pharmacology*; D010766:Phosphorylation
chemical_list: D008055:Lipids; D009525:Niacin; D000242:Cyclic AMP; D008025:Ligases; D000103:Acetyl-CoA Carboxylase
vernacular_title: Fosforilirovanie atsetil-CoA-karboksilazy pecheni tsypliat pri intensifikatsii lipogeneza i deĭstvii nikotinovoĭ kisloty.