title: A rapid computer technique for analysing molecular interactions.
abstract: A rapid method for analysing enzyme-substrate interactions using a discriminant analysis program is described. This technique identifies the structural features of substrate molecules which are important in determining metabolic activity. Two model systems, nucleoside diphosphatase activity of Golgi membranes and the interaction of yeast hexokinase with a range of D-sugars, are used as illustrations of the technique. The conclusions from both models are consistent with those previously obtained from analytical techniques.
authors.affiliation: Department of Biological Sciences, Manchester Polytechnic, UK.
authors.forename: A H
authors.initials: AH
authors.lastname: Fielding
authors.forename: C A
authors.initials: CA
authors.lastname: Smith
mesh_terms: D017766:Acid Anhydride Hydrolases; D001671:Biochemistry / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D006056:Golgi Apparatus / Q000201:enzymology; D006593:Hexokinase / Q000378:metabolism; D008432:Mathematical Computing; D008954:Models, Biological; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012984:Software
chemical_list: D006593:Hexokinase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C023837:nucleoside-diphosphatase; D005947:Glucose