Lectin binding sites of glycoproteins as revealed by lectin-gold-silver and lectin-peroxidase-diaminobenzidine methods.
For the precise histochemical detection of lectin binding sites of glycoproteins, the results obtained by lectin-gold-silver (LT-G-S) staining methods have been systematically compared with those revealed by alternative techniques of lectin-peroxidase-diaminobenzidine (LT-PO-DAB) reactions in a series of organs from different mammalian species. Ricinus communis agglutinin-I and concanavalin A were the lectins used in the present study. In the tissues subjected to the LT-G-S procedures, reactive tissue structures exhibited positive reactions of varying intensities of black. The results of control staining for the LT-G-S methods substantiated the view that the reaction products demonstrated the precise lectin binding sites of glycoproteins. The staining images obtained by the LT-PO-DAB techniques were not necessarily correlated precisely with those revealed by the LT-G-S procedures, and unavoidable background staining in pale brownish shades was noted in the majority of LT-G-S negative tissue structures. In view of these results, the LT-G-S staining methods employed in the present study are believed to be a reliable technique for the precise localization of saccharide residues of glycoproteins in light microscopy.
Fujimori
O
O
Department of Anatomy, Nagoya City University Medical School, Japan.
Sobue
H M
HM
Yamada
K
K
D015100:3,3'-Diaminobenzidine; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002417:Cattle; D003208:Concanavalin A / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D006736:Horses; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D009195:Peroxidase; D051381:Rats; D012276:Ricin / Q000378:metabolism*; D012834:Silver; D012867:Skin / Q000378:metabolism; D013552:Swine; D014470:Umbilical Cord / Q000378:metabolism
D006023:Glycoproteins; D037102:Lectins; D003208:Concanavalin A; D015100:3,3'-Diaminobenzidine; D012834:Silver; D006046:Gold; D012276:Ricin; D009195:Peroxidase