Stimulation by somatostatin of dephosphorylation of membrane proteins in pancreatic cancer MIA PaCa-2 cell line.
A membrane receptor and a cytosolic receptor for somatostatin were found in a human undifferentiated pancreatic cancer cell line (MIA PaCa-2). Binding of somatostatin to this membrane receptor activates dephosphorylation of a phosphotyrosyl-membrane protein whose phosphorylation was promoted by epidermal growth factor (EGF). Vanadate, a purported inhibitor of dephosphorylation, interferes with the action of somatostatin. These findings suggest a possible biochemical mechanism by which somatostatin may inhibit the growth of human pancreatic cancers.
Hierowski
M T
MT
Liebow
C
C
du Sapin
K
K
Schally
A V
AV
D002460:Cell Line; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology; D017481:Receptors, Somatostatin; D013004:Somatostatin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D008565:Membrane Proteins; D010750:Phosphoproteins; D011956:Receptors, Cell Surface; D017481:Receptors, Somatostatin; D014639:Vanadium; D014638:Vanadates; D013004:Somatostatin; C012088:somatostatin, N-Tyr(1)-; D004815:Epidermal Growth Factor; D010749:Phosphoprotein Phosphatases