[Isolation, purification and characteristics of brush border and basolateral membranes from cattle intestinal epithelium cells].
The surface membrane of cattle intestine epithelium cells is separated into vesicated membrane fractions of the brush border and of basolateral membranes. The brush border membrane fraction is deposited with centrifugation (15,000 g) and is localized in the layers of 45, 56.5 and 48% in the density gradient of sucrose (105,000 g). Basolateral membranes, obtained at 70,000 g, in the density gradient of sucrose are in the layers of 30 and 31.5%. The brush border membranes are 8.5 times purified, basolateral membranes--9.1 times with their insignificant contamination with subcellular elements. The both fractions are deprived of mitochondria impurities.
Tsvilikhovskiĭ
N I
NI
Usatiuk
P V
PV
Mel'nichuk
D A
DA
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D004848:Epithelium / Q000201:enzymology / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure*; D008871:Microvilli / Q000201:enzymology / Q000648:ultrastructure; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis
D000254:Sodium-Potassium-Exchanging ATPase
Vydelenie, ochistka i kharakteristika shchetochnoĭ kaĭmy i bazolateral'nykh membran iz kletok kishechnogo épiteliia krupnogo rogatogo skota.