[Effect of the membrane potential on the fusion of the Sendai viral envelope with the membrane of chick erythrocytes and on virus-induced erythrocyte hemolysis].
The ESR data on the influence of membrane potential of the fusion of Sendai virus envelope with erythrocyte membrane are presented. The hyperpolarization of cell membrane takes place at low concentration of KCl (1-5 mM) in extracellular medium in the presence of valinomycin, while at high concentration of KCl (125-150 mM) its depolarization occurs. The hyperpolarization of erythrocyte plasma membrane is accompanied by the increase of its fusion with viral envelope and virus-induced hemolysis. At the same time depolarization of erythrocyte membrane leads to the decrease of virus fusion activity. This evidence together with previously obtained by patch-clamp method data on potential-dependence of virus-induced increase of cell membrane conductivity provide us an opportunity to make a proposal that the electric field membrane damage may be the initial stage of the virus-induced membrane fusion.
Slepushkin
V A
VA
Chumakov
V M
VM
Kalmanson
A E
AE
Marchenko
S M
SM
Bukrinskaia
A G
AG
D000818:Animals; D002459:Cell Fusion; D002642:Chick Embryo; D003588:Cytopathogenic Effect, Viral; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000382:microbiology* / Q000502:physiology; D006461:Hemolysis; D008564:Membrane Potentials; D010222:Parainfluenza Virus 1, Human / Q000472:pathogenicity*; D013113:Spin Labels; D014759:Viral Envelope Proteins / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000235:genetics*
D013113:Spin Labels; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins
Vliianie membrannogo potentsiala na sliianie obolochki virusa Sendaĭ s membranoĭ kurinykh éritrotsitov i na virusindutsirovannyĭ gemoliz éritrotsitov.