Spontaneous insertion of an IS2 element into the promoter region of the lac operon.
A spontaneous mutation in pUC18 has revealed the insertion of a chromosomal insertion sequence (IS)2 element into the promoter region of the lac operon. The IS2 insertion site, at the pentanucleotide sequence TCGAG, is unlike previously described junctional sequences.
De Togni
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Department of Medicine, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111.
Fox
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Morrissey
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Babior
B M
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Levy
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SB
D001483:Base Sequence; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D004251:DNA Transposable Elements