title: In vitro characteristics of rat mesangial cells in comparison with aortic smooth muscle cells and dermal fibroblasts.
abstract: Rat glomerular mesangial cells were cultured and their antigens were compared with those of aortic vascular smooth muscle cells and dermal fibroblasts. Glomeruli, aortic, and dermal explants were cultured for 3 weeks and subcultured in the same conditions. These cultured cells were evaluated by indirect immunofluorescence studies using antibodies against Thy-1 antigen, desmin, and chicken gizzard actin. Most of mesangial cells were positive for Thy-1, desmin, and actin. On the other hand, fibroblasts were negative for desmin, and smooth muscle cells stained Thy-1 scarcely, and were negative for desmin. In the latter two cells, actin-positive fibrils were thinner and fainter than mesangial cells. These results indicated that mesangial cells could be distinguished in vitro from vascular smooth cells and fibroblasts by immunofluorescence microscopy.
authors.forename: E
authors.initials: E
authors.lastname: Yaoita
authors.forename: T
authors.initials: T
authors.lastname: Kazama
authors.forename: K
authors.initials: K
authors.lastname: Kawasaki
authors.forename: S
authors.initials: S
authors.lastname: Miyazaki
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: I
authors.initials: I
authors.lastname: Kihara
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001011:Aorta; D002478:Cells, Cultured; D003893:Desmin / Q000032:analysis; D005347:Fibroblasts / Q000032:analysis / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D005920:Glomerular Mesangium / Q000166:cytology*; D009130:Muscle, Smooth / Q000032:analysis / Q000166:cytology*; D051381:Rats; D011916:Rats, Inbred F344; D012867:Skin; D018800:Thy-1 Antigens; D013997:Time Factors
chemical_list: D000199:Actins; D000954:Antigens, Surface; D003893:Desmin; D018800:Thy-1 Antigens