Timing of chromosomal replication in Escherichia coli.
We have previously shown that certain mutations in the dnaA and recA genes of Escherichia coli perturb initiation of chromosomal replication so that all origins present are not initiated simultaneously. In this work, several genes whose protein products are involved in initiation of replication have been investigated for their effects on the synchrony of initiation. Some of the mutants (dnaC2, rpoC907, dam3) were found to have the asynchrony phenotype. Also, dnaA(Ts) mutations were shown to be dominant over dnaA+ in terms of initiation synchrony. The mechanism leading to the asynchronous phenotype is discussed.
Boye
E
E
Department of Biophysics, Institute of Cancer Research, Montebello, Oslo, Norway.
Løbner-Olesen
A
A
Skarstad
K
K
D001426:Bacterial Proteins / Q000235:genetics; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D005434:Flow Cytometry; D005798:Genes, Bacterial; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D013997:Time Factors
D001426:Bacterial Proteins