Both insulin and epidermal growth factor stimulate fatty acid synthesis and increase phosphorylation of acetyl-CoA carboxylase and ATP-citrate lyase in isolated hepatocytes.
Insulin and EGF cause identical stimulation (congruent to 40%) of fatty acid synthesis in hepatocytes isolated from rats which have been starved and then refed a low-fat diet. In both cases this stimulation is associated with increased phosphorylation of ATP-citrate lyase and of a specific site on acetyl-CoA carboxylase. However, the altered phosphorylation of acetyl-CoA carboxylase is not associated with a change in kinetic parameters which is detectable in the purified enzyme. Whatever the mechanism involved, stimulation of fatty acid synthesis by growth factors may have a role in providing new phospholipid for growth of membranes.
Holland
R
R
Hardie
D G
DG
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism*; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D008025:Ligases / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011247:Pregnancy; D051381:Rats; D013217:Starvation / Q000378:metabolism
D005227:Fatty Acids; D007328:Insulin; D010750:Phosphoproteins; D004815:Epidermal Growth Factor; D001275:ATP Citrate (pro-S)-Lyase; D008025:Ligases; D000103:Acetyl-CoA Carboxylase