[The enzymatic isolation of hepatocytes from the mouse liver].
A modified version of the two-step enzymatic method for isolation of hepatocytes from the liver of adult mice is described. The method yields 6.10(7)-7.10(7) cells with viability of 70-80%. It is simple and economical, requiring no great amounts of collagenase.
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D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D003012:Microbial Collagenase / Q000494:pharmacology
D003012:Microbial Collagenase
Fermentativnoe vydelenie gepatotsitov iz pecheni myshi.