The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
It is shown that in immunological, structural and functional terms the uncoating protein, which catalyses ATP-dependent dissociation of clathrin triskelia from clathrin-coated vesicles is intimately related to two major stress proteins of mammalian cells. These proteins of hitherto unknown functions have polypeptide mol. wts. of 73 kd and 72 kd, respectively. They are normal cell constituents which are synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues.
Ungewickell
E
E
D000818:Animals; D002352:Carrier Proteins / Q000276:immunology / Q000502:physiology*; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D002966:Clathrin / Q000276:immunology / Q000502:physiology*; D003034:Coated Pits, Cell-Membrane / Q000276:immunology / Q000502:physiology*; D003429:Cross Reactions; D003593:Cytoplasm / Q000378:metabolism; D011992:Endosomes / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000276:immunology / Q000502:physiology*; D006358:Hot Temperature; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis
D002352:Carrier Proteins; D002966:Clathrin; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D010446:Peptide Fragments