title: Competitive idiotype--anti-idiotype immunoassay for adenosine deaminase binding protein in urine.
abstract: This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reproducible as an earlier described "sandwich"-type immunoassay for ABP (Clin Chem 31: 679-683, 1985). Evaluation of urine samples from normal subjects and from patients showed increased concentrations of ABP in patients with renal disease.
authors.forename: R E
authors.initials: RE
authors.lastname: Thompson
authors.forename: C R
authors.initials: CR
authors.lastname: Hewitt
authors.forename: D J
authors.initials: DJ
authors.lastname: Piper
authors.forename: W P
authors.initials: WP
authors.lastname: Hansen
authors.forename: R H
authors.initials: RH
authors.lastname: Rubin
authors.forename: N E
authors.initials: NE
authors.lastname: Tolkoff-Rubin
authors.forename: M C
authors.initials: MC
authors.lastname: Barrett
authors.forename: M J
authors.initials: MJ
authors.lastname: Nelles
mesh_terms: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal / Q000032:analysis; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000276:immunology / Q000652:urine*; D018819:Dipeptidyl Peptidase 4; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007130:Immunoglobulin Idiotypes; D007683:Kidney Tubular Necrosis, Acute / Q000652:urine
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D007130:Immunoglobulin Idiotypes; D018819:Dipeptidyl Peptidase 4