title: A method for rapid preparation of single-cell suspensions from rat hepatocyte primary cultures on collagen substratum and the mechanism of cell dissociation.
abstract: A method has been developed for the rapid preparation of single-cell suspensions from rat hepatocyte primary cultures on collagen substratum. Hepatocytes were adequately dissociated into single cells when the cultures were first treated with a combination of trypsin and ethylenediaminetetraacetic acid (EDTA) and then with collagenase. However, when the order was reversed, hepatocytes were inadequately dispersed. The possible mechanism of cell dissociation is discussed on the basis of the experimental data obtained.
authors.affiliation: Division of Pathology, Okayama University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: J
authors.initials: J
authors.lastname: Sato
mesh_terms: D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D003094:Collagen; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D008099:Liver / Q000166:cytology*; D003012:Microbial Collagenase / Q000494:pharmacology; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D003094:Collagen; D004492:Edetic Acid; D014357:Trypsin; D003012:Microbial Collagenase