A minimum structured adenine nucleotide for ADP/ATP transport in mitochondria.
An ADP analogue, i.e. 3H- or 14C-labeled 5'-diphosphate of 6-(beta-D-ribofuranosyl-methyl)-4-pyrimidinamine, was synthesized, the structure of which was deduced from structure-activity studies on the substrate specificity of the nucleotide-binding center of the inner mitochondrial membrane integrated ADP/ATP carrier protein. Bearing only the minimal but substrate-essential recognition structures, minimum structured ADP (msADP) is bound to the cytosol-facing active center and transported by the highly specific carrier system across the inner mitochondrial membrane.
Schlimme
E
E
Institut für Chemie und Physik, Bundesanstalt für Milchforschung, Kiel.
Boos
K S
KS
Renz
H
H
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D014316:Tritium
D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D014316:Tritium; C059220:6-(ribofuranosylmethyl)-4-pyrimidinamine-5'-diphosphate; D000244:Adenosine Diphosphate; D000226:Mitochondrial ADP, ATP Translocases; D009713:Nucleotidyltransferases