Inhibitory effect of glycyrrhizin on polypeptide phosphorylation by polypeptide-dependent protein kinase (kinase P) in vitro.
The anti-viral mechanism of glycyrrhizin (GL) has been investigated by considering in vitro effects on polypeptide phosphorylation. It was found that GL (i), at low doses, selectively inhibits protein phosphorylation by Kinase P, but has not significant effects on the activities of other kinases (Kinase A, Kinase C and histone kinase); (ii) binds directly to Kinase P and reduces kinase activity in a dose-dependent manner; and (iii) inhibits vesicular stomatitis virus (VSV)-associated kinase activity. These observations strongly suggest that direct binding of GL to the virus causes the direct inactivation of virus-associated kinase and the reduction of the viral infectivity.
Ohtsuki
K
K
Department of Bioscience, Kitasato University School of Hygienic Sciences, Kanagawa, Japan.
Iahida
N
N
D002213:Capsid / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D019695:Glycyrrhizic Acid; D006367:HeLa Cells; D066298:In Vitro Techniques; D010750:Phosphoproteins / Q000378:metabolism*; D006656:Protamine Kinase / Q000037:antagonists & inhibitors; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D047428:Protein Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology; D014758:Viral Core Proteins / Q000378:metabolism; D017361:Viral Nonstructural Proteins
D002364:Caseins; D010750:Phosphoproteins; D047428:Protein Kinase Inhibitors; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D019695:Glycyrrhizic Acid; C054566:protein kinase P; D006656:Protamine Kinase; D017346:Protein Serine-Threonine Kinases; D011493:Protein Kinase C; D006034:Glycyrrhetinic Acid