Detection of cytomegalovirus in shell vial cultures by using a DNA probe and early nuclear antigen monoclonal antibody.
An in situ biotinylated DNA probe assay was evaluated as an adjunct to anti-cytomegalovirus early nuclear antigen indirect immunofluorescence and cytopathic effect on cytomegalovirus-infected monolayers in shell vial cultures. Viral infection was detected by early nuclear antigen indirect immunofluorescence at 24 h and by DNA probe assay and shell vial cytopathic effect at 5 days.
Scott
A A
AA
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425.
Walker
K A
KA
Hennigar
L M
LM
Williams
C H
CH
Manos
J P
JP
Gansler
T
T
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003588:Cytopathogenic Effect, Viral; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D017874:Immediate-Early Proteins; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015342:DNA Probes; D004279:DNA, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus