Conditions of activation of yeast plasma membrane ATPase.
The in vivo activation of the H+-ATPase of baker's yeast plasma membrane found by Serrano in 1983 was demonstrated with D-glucose aerobically and anaerobically (as well as in a respiration-deficient mutant) and, after suitable induction, with maltose, trehalose, and galactose. The activated but not the control ATPase was sensitive to oligomycin. No activation was possible in a cell-free extract with added glucose. The ATPase was not activated in yeast protoplasts which may account for the absence of glucose-stimulated secondary active transports in these wall-less cells and provide support for a microscopic coupling between ATPase activity and these transports in yeast cells.
Sychrová
H
H
Kotyk
A
A
D002462:Cell Membrane / Q000201:enzymology; D004054:Diethylstilbestrol / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D008320:Maltose / Q000494:pharmacology; D009840:Oligomycins / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011523:Protoplasts / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014199:Trehalose / Q000494:pharmacology; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D009840:Oligomycins; D014639:Vanadium; D014638:Vanadates; D008320:Maltose; D004054:Diethylstilbestrol; D014199:Trehalose; D006180:Proton-Translocating ATPases; D005947:Glucose; D005690:Galactose