Monensin-resistant mouse Balb/3T3 cell mutant with aberrant penetration of vesicular stomatitis virus.
A mutant (MO-5) resistant to monensin (an ionophoric antibiotic) derived from the mouse Balb/3T3 cell line, was a poor host for vesicular stomatitis virus (VSV) or semliki forest virus (SFV) multiplication. The yield of VSV particles in MO-5 is one 100-fold reduced as is VSV-dependent RNA synthesis. In contrast to a pH-remedial mutant, the abortive production of infectious VSV particles in MO-5 cells was not restored by low pH treatment. The pH values in the endosome and the lysosome of MO-5 cells were 5.2 and 5.4, respectively, values that were comparable to the pH value in Balb/3T3 cells. Assays with [3H]uridine-labeled VSV indicated similar binding of VSV in MO-5: percoll gradient centrifugation analysis of [35S]methionine-labeled VSV-infected Balb/3T3 showed accumulation of VSV in the lysosome fraction 20 min after VSV infection, whereas VSV can be found mainly in endosome/Golgi fraction of MO-5 cells after 40 to 60 min on the percoll gradients. Degradation of [35S]methionine-labeled VSV was observed at a significant rate in Balb/3T3 cells, but not in MO-5 cells. The monensin-resistant somatic cell may thus provide a genetic route to study the mechanism of endocytosis or transport of enveloped viruses.
Ono
M
M
Mifune
K
K
Yoshimura
A
A
Ohnishi
S
S
Kuwano
M
M
D000818:Animals; D002451:Cell Compartmentation; D002460:Cell Line; D004351:Drug Resistance; D004705:Endocytosis; D011992:Endosomes / Q000502:physiology; D005663:Furans / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008985:Monensin / Q000494:pharmacology*; D009154:Mutation; D012367:RNA, Viral / Q000096:biosynthesis; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development*; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D005663:Furans; D012367:RNA, Viral; D014764:Viral Proteins; D008985:Monensin