Ionic regulation of the binding of DL-2-amino-7-phosphono-[4,5-3H]heptanoic acid to synaptosome-enriched homogenates of rat cerebral cortex.
The ionic requirements of the site labelled by DL-2-amino-7-phosphono-[4,5-3H]heptanoic acid ([3H]DL-2AP7) in synaptosome-enriched homogenates of rat cerebral cortex were examined using radioligand binding methodology. Binding of [3H]DL-2AP7 was increased by calcium and chloride ions by an apparently non-competitive mechanism. The actions of ions on specific binding displayed similarities to the effects of ions on DL-[3H]2-amino-4-phosphonobutyric acid and Cl-/Ca2+-dependent L-[3H]glutamate ([3H]Glu) binding sites but were more consistent with the Glu-C binding site.
Manallack
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Beart
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D015763:2-Amino-5-phosphonovalerate / Q000031:analogs & derivatives*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism*; D002712:Chlorides / Q000502:physiology*; D005615:Freezing; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism*
D000596:Amino Acids; D002712:Chlorides; D005971:Glutamates; D018698:Glutamic Acid; D015763:2-Amino-5-phosphonovalerate; C031231:2-amino-7-phosphonoheptanoic acid; D002118:Calcium