Brain and peripheral opioid peptides after changes in ingestive behavior.
The levels of dynorphin, alpha-neoendorphin and beta-endorphin immunoreactivity (ir-DYN, ir-alpha-NEO, ir-beta-E) were measured in the brain, pituitary and gut of rats subjected to a variety of manipulations. Water deprivation caused an increase in the ir-DYN and ir-alpha-NEO content in the hypothalamus and a decrease in the neurointermediate (NI) lobe of the pituitary. The ir-beta-E level decreased in the hypothalamus and anterior lobe of the pituitary, while it increased in the NI-pituitary. Food deprivation, as well as chronic fenfluramine (10-20 mg/kg) treatment increased, while acute muscimol (0.5 micrograms/10 microliter) treatment decreased the ir-beta-E, but not ir-DYN or ir-alpha-NEO content in the hypothalamus. The anterior pituitary content of ir-beta-E was increased after food deprivation and decreased after chronic fenfluramine treatment. However, the ir-DYN and ir-alpha-NEO contents in the duodenum were markedly increased after food deprivation, while chronic fenfluramine treatment led to a dramatic decrease in the ir-DYN content. These results suggest that the levels of opioid peptides in the brain, pituitary and gut may be differentially and independently affected by alteration of the ingestive behavior.
Majeed
N H
NH
Lasoń
W
W
Przewłocka
B
B
Przewłocki
R
R
D000818:Animals; D003681:Dehydration / Q000378:metabolism; D004386:Duodenum / Q000032:analysis*; D004399:Dynorphins / Q000032:analysis; D004723:Endorphins / Q000032:analysis*; D005508:Food Deprivation / Q000502:physiology*; D007031:Hypothalamus / Q000032:analysis*; D008297:Male; D010902:Pituitary Gland / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014868:Water Deprivation / Q000502:physiology*; D001615:beta-Endorphin
D004723:Endorphins; D011498:Protein Precursors; D001615:beta-Endorphin; C025681:alpha-neoendorphin; D004399:Dynorphins