title: Metabolism of 7,12-dimethylbenz(a)anthracene and its DNA adduct formation in human fetal kidney and intestinal cells in culture.
abstract: Epithelial cell cultures derived from human fetal intestine and kidney were analyzed for their capability to metabolize 7,12-dimethylbenz(a)anthracene (DMBA) and form DNA-DMBA adducts. Both the intestinal and kidney cells were able to metabolize DMBA to water and organic soluble metabolites and formed DMBA-DNA adducts. Intestinal cells metabolized 39.5 +/- 25.2% of the DMBA to organic soluble products and 2.9 +/- 0.4% to water-soluble metabolites after 24-hr incubation. Kidney cells yielded 27.4 +/- 18.1 and 3.8 +/- 2.7% organic and water-soluble metabolites, respectively. Kidney cells appeared to produced larger amounts of 7,12-dihydroxymethylbenz(a)anthracene and DMBA-8,9-dihydrodiols than intestinal cell cultures, while intestinal cells produced greater amounts of phenol metabolites. The level of DNA-DMBA adducts formed in the intestinal and kidney cell cultures after 24-hr incubation were 20.4 +/- 17.1 and 36.7 +/- 25.3 mumol DMBA/mol DNA-phosphate, respectively. Major elution peaks were observed where the DMBA-1,2-epoxide-3,4-dihydrodiol-deoxyguanosine adduct eluted. These data indicate qualitatively similar, but quantitatively different, levels of DMBA metabolites and DMBA-DNA adducts produced by human fetal intestinal and kidney epithelial cells in culture.
authors.forename: C T
authors.initials: CT
authors.lastname: Oravec
authors.forename: M J
authors.initials: MJ
authors.lastname: Samuel
authors.forename: S M
authors.initials: SM
authors.lastname: D'Ambrosio
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D005333:Fetus; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*
chemical_list: D015127:9,10-Dimethyl-1,2-benzanthracene; D004247:DNA