Lipid esterification and secretion by the mouse intestine in organ culture.
Explants of adult mouse jejunum were cultured for different time periods and incubated in presence of a lipid diet emulsified by sodium taurocholate or complexed with albumin. The esterification of fatty acids and the secretion of triglycerides and phospholipids were measured and compared to the lipid absorption observed in vivo after the perfusion of the same diet. The results show that, in vitro, the enterocytes esterify the fatty acids present in the medium and secrete them with a yield improving during the culture and even better than the absorption observed in the in vivo experiments.
Carlier
H
H
Labussiere
H
H
Bernard
A
A
Hugon
J S
JS
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D004952:Esters; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D009924:Organ Culture Techniques; D010743:Phospholipids / Q000096:biosynthesis; D013393:Sucrase / Q000378:metabolism; D013656:Taurocholic Acid / Q000494:pharmacology; D014280:Triglycerides / Q000096:biosynthesis; D000520:alpha-Glucosidases / Q000378:metabolism
D004952:Esters; D009829:Oleic Acids; D010743:Phospholipids; D014280:Triglycerides; D019301:Oleic Acid; D013656:Taurocholic Acid; D000469:Alkaline Phosphatase; D000520:alpha-Glucosidases; D013393:Sucrase