title: Ornithine decarboxylase and phosphatase activity can be stimulated by low concentrations of interferon in human breast cancer cell lines.
abstract: The activity of the enzyme ornithine decarboxylase (ODC) is increased in confluent culture of two breast cancer cell lines (T47D and MCF-7) following a change of medium. The increase in activity is maximum at 12 hours and this level is reduced in cells treated with high concentrations of HuIFN-alpha. However, the increased level of ODC activity seen in the first few hours after medium change can be stimulated by low concentrations of interferon (10-100 units per ml). The activity of the acidic and alkaline phosphatase of the above cell lines are affected by HuIFN-alpha similar to ODC. In stimulating levels of ODC and phosphatases, interferon is acting similar to mitogens, and in T47D and MCF-7 cells this stimulatory effect precedes the inhibitory effect more commonly seen in interferon-treated cells.
authors.affiliation: Theagenion Cancer Institute, Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Kortsaris
authors.forename: D A
authors.initials: DA
authors.lastname: Kyriakidis
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D001943:Breast Neoplasms; D002285:Carcinoma, Intraductal, Noninfiltrating; D002460:Cell Line; D005260:Female; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D007370:Interferon Type I; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D009955:Ornithine Decarboxylase