Cloning and sequence analysis of the cDNA encoding a snake neurotoxin precursor.
A recombinant plasmid has been constructed containing a sequence of 186 nucleotides encoding a potent neurotoxin found in the venom of the sea-snake Laticauda semifasciata and designated as erabutoxin a. This sequence is flanked, in the upstream region, by a sequence of 60 nucleotides encoding a hydrophobic peptide fragment presumably involved in the secretion process of the neurotoxin. The sequence coding for the toxin ends with a termination codon which is followed by a 3'-untranslated sequence of approximately 240 nucleotides (excluding the poly(A) tract).
Tamiya
T
T
Lamouroux
A
A
Julien
J F
JF
Grima
B
B
Mallet
J
J
Fromageot
P
P
Ménez
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004546:Elapid Venoms / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004870:Erabutoxins / Q000235:genetics*; D011061:Poly A / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism
D004546:Elapid Venoms; D011498:Protein Precursors; D012333:RNA, Messenger; C038908:erabutoxin A; D011061:Poly A; D004870:Erabutoxins; D004247:DNA