title: Avian (chicken) parathyroid hormone: synthesis and comparative biological evaluation of the 1-34 fragment.
abstract: The full-length amino acid sequence of the avian (chicken) form of parathyroid hormone (cPTH) has recently been elucidated. We have chemically synthesized, purified to a high degree, and analytically and biologically characterized the N-terminal 1-34 fragment of the avian hormone. The biological properties of cPTH-(1-34)NH2 were evaluated and compared to the bovine fragment bPTH-(1-34) in several assays. The potency of cPTH-(1-34)NH2 in binding to PTH receptors, in stimulating adenylate cyclase activity and in relaxing smooth muscle tissue was approximately one-tenth that of bPTH-(1-34). Comparison of the avian sequence to other native PTH related sequences suggests that changes in the binding domain of the 1-34 active fragment may account for the decline in potency.
authors.affiliation: Department of Biological Research and Molecular Biology, Merck Sharp & Dohme Research Laboratories, West Point, Pennsylvania 19486.
authors.forename: M P
authors.initials: MP
authors.lastname: Caulfield
authors.forename: J J
authors.initials: JJ
authors.lastname: Levy
authors.forename: R L
authors.initials: RL
authors.lastname: McKee
authors.forename: M E
authors.initials: ME
authors.lastname: Goldman
authors.forename: P A
authors.initials: PA
authors.lastname: DeHaven
authors.forename: J E
authors.initials: JE
authors.lastname: Reagan
authors.forename: L
authors.initials: L
authors.lastname: Heaney
authors.forename: R F
authors.initials: RF
authors.lastname: Nutt
authors.forename: R J
authors.initials: RJ
authors.lastname: Winquist
authors.forename: J
authors.initials: J
authors.lastname: Russell
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D002645:Chickens / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D010281:Parathyroid Hormone / Q000032:analysis / Q000138:chemical synthesis / Q000494:pharmacology*; D010446:Peptide Fragments / Q000032:analysis / Q000138:chemical synthesis / Q000494:pharmacology*; D011817:Rabbits; D012077:Renal Artery / Q000187:drug effects / Q000502:physiology; D014664:Vasodilation / Q000187:drug effects
chemical_list: D000596:Amino Acids; D010281:Parathyroid Hormone; D010446:Peptide Fragments; C025076:parathyroid hormone (1-34), bovine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases