title: Inhibition of growth of Leishmania mexicana mexicana by Leishmania mexicana amazonensis during "in vitro" co-cultivation.
abstract: Inhibition of one Leishmania subspecies by exometabolites of another subspecies, a phenomenon not previously reported, is suggested by our recent observations in cell cloning experiments with Leishmania mexicana mexicana and Leishmania mexicana amazonensis. Clones were identified using the technique of schizodeme analysis. The phenomenon observed is clearly relevant to studies of parasite isolation, leishmanial metabolism, cross-immunity and chemotherapy.
authors.affiliation: Instituto Oswaldo Cruz, Departamento de Bioquímica e Biologia Molecular, Rio de Janeiro, Brasil.
authors.forename: R S
authors.initials: RS
authors.lastname: Pacheco
authors.forename: G
authors.initials: G
authors.lastname: Grimaldi Júnior
authors.forename: C M
authors.initials: CM
authors.lastname: Morel
mesh_terms: D000818:Animals; D003470:Culture Media; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004270:DNA, Circular / Q000032:analysis*; D018105:DNA, Kinetoplast; D004591:Electrophoresis, Polyacrylamide Gel; D006131:Growth Inhibitors / Q000378:metabolism*; D066298:In Vitro Techniques; D007894:Leishmania mexicana / Q000254:growth & development* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D003470:Culture Media; D004270:DNA, Circular; D018105:DNA, Kinetoplast; D006131:Growth Inhibitors; D004262:DNA Restriction Enzymes