Biochemical studies of neutrophils from male and female rats: a differential response to basement membrane treated with nephrotoxic antiserum.
Rat neutrophils added to 3H-labelled glomerular basement membrane (GBM) treated with rabbit anti-rat GBM antiserum degraded the GBM as judged by the release of 3H-labelled peptides. Cells from female animals promoted a more marked degradation than cells from males. This correlated with measurements of higher levels of elastase in granule fractions from the cells. The subcellular distributions of granule marker enzymes was found not to differ between the sexes. Levels of myeloperoxidase, lysozyme, cathepsin G, alkaline phosphatase, gamma-glutamyltranspeptidase and N-acetyl-beta-glucosaminidase showed no sex-based differences. No alpha-mannosidase could be detected in the cells.
Unarska-Jachimczyk
M
M
Department of Biochemistry, University of Oxford, U.K.
Robinson
G B
GB
Keer
J
J
D000818:Animals; D000906:Antibodies; D001485:Basement Membrane / Q000276:immunology / Q000502:physiology*; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000648:ultrastructure; D005260:Female; D006867:Hydrolases / Q000378:metabolism; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors
D000906:Antibodies; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D006867:Hydrolases