Presence of ACTH and its receptor on a B lymphocytic cell line: a possible autocrine function for a neuroendocrine hormone.
A mouse B lymphocytic cell line, designated BCL1, was found to produce immunoreactive ACTH and to secrete this molecule into culture supernates. The BCL1-derived ACTH induced Y-1 adrenal cells to undergo a steroidogeneic response and was eluted from gel filtration columns at a molecular weight similar to that expected for pituitary-derived ACTH. Furthermore, ACTH receptors were detected on the surface of BCL1 cells using indirect immunofluorescence and 125I-ACTH binding studies. Scatchard analysis demonstrated the presence of high and low affinity binding sites with dissociation constants of 4.5 x 10(-12) M and 2.8 x 10(-9) M, respectively. The production of both ACTH and its receptor by this B lymphocyte cell line suggests that an autocrine mechanism might be important for the maintenance of the transformed phenotype.
Bost
K L
KL
Department of Physiology and Biophysics, University of Alabama, Birmingham.
Smith
E M
EM
Wear
L B
LB
Blalock
J E
JE
D000324:Adrenocorticotropic Hormone / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D002460:Cell Line; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D051379:Mice; D018043:Receptors, Corticotropin; D011979:Receptors, Pituitary Hormone / Q000032:analysis* / Q000276:immunology
D018043:Receptors, Corticotropin; D011979:Receptors, Pituitary Hormone; D000324:Adrenocorticotropic Hormone