title: The gene for yeast ribosomal protein S31 contains an intron in the leader sequence.
abstract: Analysis of the primary structure of the gene for yeast ribosomal protein S31 revealed two unusual features. First, an intron of 312 nucleotides is located within the 5'-untranslated region. Second, the coding sequence for the known amino-terminal peptide of the protein starts 13 codons downstream of the ATG initiation codon, suggesting that S31 is synthesized as a precursor which undergoes post-translational processing to the mature protein. Primer extension analysis showed that transcription of the S31 gene starts at multiple sites. The 5'-flanking region of the gene contains several, previously described, conserved sequence elements that may play a role in the coordinate expression of yeast ribosomal protein genes.
authors.affiliation: Biochemisch Laboratorium, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: R T
authors.initials: RT
authors.lastname: Nieuwint
authors.forename: C M
authors.initials: CM
authors.lastname: Molenaar
authors.forename: J H
authors.initials: JH
authors.lastname: van Bommel
authors.forename: M M
authors.initials: MM
authors.lastname: van Raamsdonk-Duin
authors.forename: W H
authors.initials: WH
authors.lastname: Mager
authors.forename: R J
authors.initials: RJ
authors.lastname: Planta
mesh_terms: D004262:DNA Restriction Enzymes; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D012269:Ribosomal Proteins / Q000235:genetics*; D012440:Saccharomyces / Q000235:genetics*
chemical_list: D012269:Ribosomal Proteins; D004262:DNA Restriction Enzymes