[Isolation and characteristics of single-stranded DNA-binding protein (SSB-protein) from Ehrlich ascites carcinoma cells].
Single-stranded DNA-binding protein (SSB-protein) has been purified and characterized from Ehrlich ascite tumour (EAT) cells. The purification procedure was performed in analytical and preparative variants. It was shown that in the analytical variant of the purification procedure can be used to determine protein concentration in the cell. The molecular mass of the SSB-protein as determined by SDS polyacrylamide gel electrophoresis is 36 and 43 kD; that determined by gel filtration is 27, 28, 43 and 44 kD; pI is 7.4. The use of nitrocellulose filters showed that the SSB-protein binds preferentially to ss-DNA. The protein contains no admixtures of DNA-polymerases, endo- or exonucleases, DNA-dependent ATPase, lactate dehydrogenase and HMG-proteins. The SSB-protein stimulates 1.5-2-fold the activity of DNA-polymerase alpha from EAT, it does not activate DNA-polymerase beta from EAT and strongly inhibits the activity of exonuclease (snake venom phosphodiesterase). The specificity of the term "SSB-protein" which makes it different from other non-histone proteins of chromatin is discussed.
Koterov
A N
AN
Novoradovskaia
N A
NA
Tronov
V A
VA
Zolotareva
L A
LA
Filippovich
I V
IV
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004720:Endonucleases / Q000037:antagonists & inhibitors; D006609:High Mobility Group Proteins / Q000302:isolation & purification; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors; D014407:Tumor Cells, Cultured / Q000032:analysis
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D004259:DNA-Directed DNA Polymerase; D004720:Endonucleases
Vydelenie i kharakteristika belka, sviazyvaiushchegosia s odnonitevoĭ DNK (SSB-belka) iz kletok astsitnoĭ kartsinomy Erlikha (AKE).