Fructose 2,6-bisphosphate and its phosphorothioate analogue. Comparison of their hydrolysis and action on glycolytic and gluconeogenic enzymes.
Purified chicken liver 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase was phosphorylated either from fructose 2,6-bis[2-32P]phosphate or fructose 2-phosphoro[35S]thioate 6-phosphate. The turnover of the thiophosphorylated enzyme intermediate as well as the overall phosphatase reaction was four times faster than with authentic fructose 2,6-bisphosphate. Fructose 2-phosphorothioate 6-phosphate was 10-100-fold less potent than authentic fructose 2,6-bisphosphate in stimulating 6-phosphofructo-1-kinase and pyrophosphate:fructose 6-phosphate phosphotransferase, but about 10 times more potent in inhibiting fructose 1,6-bisphosphatase. The analogue was twice as effective as authentic fructose 2,6-bisphosphate in stimulating pyruvate kinase from trypanosomes.
Rider
M H
MH
Institute of Cellular and Molecular Pathology, Louvain University Medical School, Brussels, Belgium.
Kuntz
D A
DA
Hue
L
L
D000818:Animals; D002645:Chickens; D005635:Fructosediphosphates / Q000378:metabolism*; D006019:Glycolysis; D006598:Hexosediphosphates / Q000378:metabolism*; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000378:metabolism; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism
D005635:Fructosediphosphates; D006598:Hexosediphosphates; C057408:fructose 2-phosphorothioate 6-phosphate; C027652:fructose 2,6-diphosphate; D010770:Phosphotransferases; D025481:Phosphofructokinase-2; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; C022502:pyrophosphate-fructose 6-phosphate 1-phosphotransferase; D010744:Phosphoric Monoester Hydrolases