title: Reconstitution of partially purified opioid receptors with a GTP-binding protein.
abstract: Partially purified opioid receptors, obtained from rat brains using an affinity resin, AF-Amino Toyopearl with [D-Ala2, Leu5]enkephalin, were reconstituted with an inhibitory GTP-binding protein (Gi). In the reconstituted system, the displacement curve for the binding of a delta-agonist, [D-Ala2, D-Leu5]enkephalin, showed two states, high and low affinity binding ones, with different affinities for the agonist. The high affinity binding was eliminated by the addition of a guanine nucleotide analog to the system. These results directly showed that opioid receptors, at least the delta-type, could interact with Gi.
authors.affiliation: Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujioka
authors.forename: F
authors.initials: F
authors.lastname: Inoue
authors.forename: S
authors.initials: S
authors.lastname: Sumita
authors.forename: M
authors.initials: M
authors.lastname: Kuriyama
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007700:Kinetics; D051381:Rats; D011957:Receptors, Opioid / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D011957:Receptors, Opioid; D006165:Guanylyl Imidodiphosphate; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; C030279:enkephalinamide-Leu, Ala(2)-; D019204:GTP-Binding Proteins