[Effect of detergents on the solubilization of 5'-nucleotidase from the thyroid gland].
The effect of Triton X-100, sodium deoxycholate and saponin upon the solubilization of 5'-nucleotidase, the membrane enzyme derived from pig thyroid was studied. Triton X-100 at the concentration of 0.1% did not cause enzyme solubilization, whereas at the concentration of 1.0% it caused only partial release of the former from the membranes. Saponin (1.0% concentration) brought about a marked (about threefold) increase in the enzyme activity which resulted from the exposing active loci of the enzyme, however it did not cause total solubilization of it. 1% sodium deoxycholate increased the enzyme activity by 5 times and also caused its almost total solubilization (over 90%). The results indicate the 5'-nucleotidase is strongly bound to the cell membranes and the non-ionic detergents like Triton X-100 and saponin do not fit for the solubilization of this enzyme.
Piotrowska
E
E
Zakładu Chemii Fizycznej, Instytutu Chemii AM w Białymstoku.
Niedźwiecka
J
J
Jaroszewicz
L
L
D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology; D012995:Solubility; D013552:Swine; D013961:Thyroid Gland / Q000187:drug effects / Q000201:enzymology*
D003902:Detergents; D012503:Saponins; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D017830:Octoxynol; D015720:5'-Nucleotidase
Wpływ detergentów na solubilizacje 5'-nukleotydazy z tarczycy.