In vitro transport of F1-ATPase beta-subunit into mitochondria of Zajdela hepatoma and rat liver.
Transport of precursor of F1-ATPase beta-subunit into isolated mitochondria of Zajdela hepatoma and rat liver was examined. The hepatoma mitochondria were more active in the process than the liver organelles indicating that the relative F1-ATPase deficiency in the tumor mitochondria does not result from an impaired transport of F1-ATPase subunits into the tumor organelles. Similar results were obtained using digitonin-treated rat hepatocytes and Zajdela hepatoma cells instead of isolated mitochondria. The suitability of the digitonin-treated cells in the study of protein transport into mitochondria in vitro is demonstrated and the advantages of this system over isolated mitochondria are discussed.
Kuzela
S
S
Luciaková
K
K
Lakota
J
J
D000818:Animals; D001692:Biological Transport; D003599:Cytoskeleton / Q000502:physiology; D004072:Digitonin / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism*; D046911:Macromolecular Substances; D008930:Mitochondria, Liver / Q000201:enzymology; D011499:Protein Processing, Post-Translational; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats
D046911:Macromolecular Substances; D006180:Proton-Translocating ATPases; D004072:Digitonin