Presence of dynorphin-like immunoreactivity but not opiate binding in Walker-256 tumors.
Walker-256 tumor tissue was removed from rats on day 8 of tumor growth. An acidified methanol extract of the tumor tissue was assayed for immunoreactive (ir) dynorphin-A 1-17 (DYN-17) and ir-dynorphin-A (DYN-8). Levels of ir-DYN-17 and ir-DYN-8 were nearly 4-and 8-fold higher, respectively, in tumors versus normal muscle. However, tumor homogenates did not exhibit specific 3H-naloxone binding. These results indicate that although the Walker-256 carcinosarcoma may produce opioids, it is unlikely that these ectopic substances have direct opioid actions on the tumor itself.
Bryant
H U
HU
Conroy
W G
WG
Isom
G E
GE
Malven
P V
PV
Yim
G K
GK
D000818:Animals; D002279:Carcinoma 256, Walker / Q000032:analysis* / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D004399:Dynorphins / Q000032:analysis*; D004723:Endorphins / Q000502:physiology; D006729:Hormones, Ectopic / Q000502:physiology; D008297:Male; D009132:Muscles / Q000032:analysis; D009270:Naloxone / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000032:analysis*
D004723:Endorphins; D006729:Hormones, Ectopic; D010446:Peptide Fragments; D011957:Receptors, Opioid; D009270:Naloxone; D004399:Dynorphins; C026770:dynorphin (1-8)