Functional expression of the oxytocin receptor in Xenopus laevis oocytes primed with mRNA from bovine endometrium.
Synthesis of the uterine receptor for the hypothalamic hormone oxytocin has been induced in oocytes from Xenopus laevis previously primed with bovine endometrium mRNA. The injected oocytes responded to oxytocin by showing dose-dependent oscillations in membrane currents as recorded by the voltage-clamp method. The response was specific in that it was not elicited by several other peptides tested. The oxytocin-induced membrane changes were suppressed when oocytes were pretreated with an oxytocin receptor antagonist.
Morley
S D
SD
Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Federal Republic of Germany.
Meyerhof
W
W
Schwarz
J
J
Richter
D
D
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000032:analysis*; D005260:Female; D008564:Membrane Potentials / Q000187:drug effects; D008845:Microinjections; D009865:Oocytes / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D012333:RNA, Messenger / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D011945:Receptors, Angiotensin / Q000378:metabolism* / Q000502:physiology; D018045:Receptors, Oxytocin; D014982:Xenopus laevis / Q000502:physiology*
D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D018045:Receptors, Oxytocin