title: Adhesion of Pseudomonas aeruginosa to human buccal epithelial cells: evidence for two classes of receptors.
abstract: The adhesion of Pseudomonas aeruginosa strain 492c to trypsinized and untrypsinized buccal epithelial cells (BECs) was studied. Kinetic analysis of the adhesion data, employing a Langmuir absorption isotherm, indicated the presence of two classes of binding sites on untrypsinized BECs: a high affinity - low copy number site (apparent association constant (Ka) approximately equal to 1.57 X 10(-8) mL/cell with ca. 29 binding sites/cell) and a low affinity - high copy number class of binding sites (Ka approximately equal to 4.78 X 10(-10) mL/cell with ca. 264 binding sites/cell). The low affinity - high copy number class of sites was found to be trypsin sensitive. A single class of binding sites was found on trypsinized BECs exhibiting a high affinity - low copy number (Ka approximately equal to 3.70 X 10(-7) mL/cell with ca. 31 binding sites/cell). Positive cooperativity in binding of P. aeruginosa strain 492c to the low affinity - high copy number class site on untrypsinized BECs was demonstrated by analysis of Hill plots of the adhesion data. Sugar inhibition data using a preincubation methodology showed an inhibition of adhesion to trypsinized BECs in the presence of N-acetylneuraminic acid and D-arabinose, while these same two sugars enhanced adhesion to untrypsinized BECs. D-Galactose and N-acetylglucosamine enhanced adhesion to both types of BECs though the latter did to different extents. D-Fucose only inhibited adhesion to untrypsinized BECs.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.forename: D W
authors.initials: DW
authors.lastname: McEachran
authors.forename: R T
authors.initials: RT
authors.lastname: Irvin
mesh_terms: D000268:Adhesiveness; D000464:Alginates / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002610:Cheek; D004848:Epithelium / Q000378:metabolism / Q000382:microbiology; D010861:Fimbriae, Bacterial / Q000378:metabolism; D005407:Flagella / Q000502:physiology; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D007700:Kinetics; D008297:Male; D009005:Monosaccharides / Q000494:pharmacology; D009061:Mouth Mucosa / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000502:physiology* / Q000648:ultrastructure; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000464:Alginates; D000911:Antibodies, Monoclonal; D006603:Hexuronic Acids; D009005:Monosaccharides; D020723:Glucuronic Acid; D014357:Trypsin