Norepinephrine stimulates the production of inositol trisphosphate and inositol tetrakisphosphate in rat aorta.
Norepinephrine stimulated the rapid hydrolysis of [3H]phosphatidylinositol-4,5-bisphosphate in rat aorta with a maximal decrease of 30% within 60 sec of stimulation. Levels of [3H]phosphatidylinositol-4,5-bisphosphate returned to control by 5 min despite the continued presence of agonist. Hydrolysis of [3H]phosphatidylinositol-4,5-bisphosphate occurred concurrently with the formation of inositol phosphates. Inositol-tris and tetrakisphosphate levels were increased within 30 sec of agonist stimulation. Increases in inositol phosphate levels due to agonist were dose-dependent with half-maximal activation at 1 microM norepinephrine.
Pijuan
V
V
Department of Pharmacology, University of Miami School of Medicine, Florida 33101.
Litosch
I
I
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism*; D002712:Chlorides / Q000494:pharmacology; D066298:In Vitro Techniques; D007294:Inositol / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000096:biosynthesis*; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013403:Sugar Phosphates / Q000096:biosynthesis*
D002712:Chlorides; D007295:Inositol Phosphates; D013403:Sugar Phosphates; D007294:Inositol; D015544:Inositol 1,4,5-Trisphosphate; D008094:Lithium; D018021:Lithium Chloride; D009638:Norepinephrine