Structure of the gamma/delta T cell receptor of a human thymocyte clone.
The CD3+, IL-2-dependent normal human thymocyte clone, CII, expresses on its surface a CD3-associated gamma/delta TCR. We have further elucidated the structure of this receptor from the nucleotide sequence of cDNA and genomic clones from CII that encode functional TCR-gamma and -delta chains. We find that the CII line expresses a C gamma 2 constant region that is a polymorphic form lacking a copy of an internal exon; the sequence of this constant region accounts for the size of the gamma chain and noncovalent linkage of gamma and delta chains in the CII TCR. The V gamma region used for the CII TCR is identical to the several previously characterized expressed human V gamma segments. Possible implications of this finding are discussed.
Okada
A
A
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, New York 10032.
Bank
I
I
Rogozinski
L
L
Takihara
Y
Y
Mak
T W
TW
Chess
L
L
Alt
F W
FW
D001483:Base Sequence; D015139:Blotting, Southern; D002999:Clone Cells; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D015329:Gene Rearrangement, T-Lymphocyte; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D004247:DNA