title: Adrenocorticotropin binding activity of B16/C3 melanoma cells.
abstract: The binding of adrenocorticotropic hormone (ACTH) to B16/C3 murine melanoma cells was found to be specific and saturable. The binding capacity of the cells changed as a function of the age of the culture. Scatchard analysis revealed one class of high-affinity ACTH binding sites. The specificity of ACTH binding to the cells was tested by displacement experiments with human leukocyte interferon (alpha-IFN) and alpha-melanocyte stimulating hormone (alpha-MSH) as the competitors. Structure-activity relationship of ACTH, alpha-MSH and alpha-IFN was discussed.
authors.affiliation: Institute for Biological Research, Belgrade, Yugoslavia.
authors.forename: J
authors.initials: J
authors.lastname: Simić-Krstić
authors.forename: L
authors.initials: L
authors.lastname: Sevaljević
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000493:pharmacokinetics*; D000818:Animals; D001667:Binding, Competitive; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D000324:Adrenocorticotropic Hormone