Halothane induced disorder of red cell membranes of subjects susceptible to malignant hyperthermia.
Membrane fluidity of red blood cells drawn from malignant hyperthermic pigs and humans was studied using spin-probes and electron paramagnetic resonance technique. The order parameter and rotational correlation time were determined with 12-doxylstearate and 16-doxylstearate, respectively. It was found that halothane decreased both parameters, but that the decrease of these parameters in subjects susceptible to malignant hyperthermia was much greater than that in normal subjects. The differences were most pronounced at 3 mM halothane. A possibility of using blood for a non-invasive screening for malignant hyperthermia is discussed.
Ohnishi
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Membrane Research Institute, Philadelphia, PA 19104.
Ohnishi
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D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects*; D006221:Halothane / Q000633:toxicity*; D006801:Humans; D008305:Malignant Hyperthermia / Q000097:blood* / Q000235:genetics; D008560:Membrane Fluidity / Q000187:drug effects; D013113:Spin Labels; D013552:Swine
D013113:Spin Labels; D006221:Halothane