Human bronchial epithelial cells with integrated SV40 virus T antigen genes retain the ability to undergo squamous differentiation.
Human bronchial epithelial cells transformed by either DNA virus infection (SV40 or Adenovirus 12-SV40 hybrid virus) or transfection with the SV40 large T antigen gene were studied for their ability to undergo squamous differentiation when exposed to 12-O-tetradecanoylphorbol-13 acetate (TPA), transforming growth factor-beta 1 (TGF-beta 1), or fetal bovine serum (FBS), agents that induce the squamous differentiation of normal human bronchial epithelial cells. Squamous differentiation occurred in all ten T-antigen-positive cell cultures when they were exposed to either FBS or TGF-beta 1, but none differentiated when exposed to TPA. From one cell line, designated BEAS-2B, two subclones were isolated, one of which was induced to undergo squamous differentiation by FBS, and a second that failed to undergo squamous differentiation and was mitogenically stimulated when exposed to serum. These phenotypically different subclones provide a new in vitro cellular system for delineating the mechanism(s) of human bronchial epithelial cell squamous differentiation in response to FBS or TGF-beta 1.
Ke
Y
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Division of Cancer Etiology, National Cancer Institute, Bethesda, MD 20892.
Reddel
R R
RR
Gerwin
B I
BI
Miyashita
M
M
McMenamin
M
M
Lechner
J F
JF
Harris
C C
CC
D000956:Antigens, Viral / Q000378:metabolism*; D001980:Bronchi / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006801:Humans; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
D000956:Antigens, Viral; D012710:Serum Albumin, Bovine; C032975:12-O-tetradeca-2,4,6,8-tetranoylphorbol-13-acetate; D015291:Transforming Growth Factors; D013755:Tetradecanoylphorbol Acetate