Identification of a specific receptor for erythroid differentiation factor on follicular granulosa cell.
A cellular receptor for erythroid differentiation factor (EDF) was demonstrated by incubation of 125I-labeled EDF with rat follicular granulosa cell cultures. The specific binding of labeled EDF to the cells showed saturation; Scatchard analysis of binding data indicated a single class of receptors having Kd = 3.4 x 10(-10) M. A large excess of unlabeled EDF reduced labeled EDF binding almost completely, whereas similar doses of inhibin and transforming growth factor type beta, which are quite similar to EDF in protein structure and subunit organization, had no effect; EDF did not share receptors with those factors. EDF receptor (activin A receptor) expression was enhanced in granulosa cells cultured in the presence of follicle-stimulating hormone; follicle-stimulating hormone raised the number of EDF binding sites/cell from 13,000 to 96,000 without altering the binding affinity.
Sugino
H
H
Frontier Research Program, Institute of Physical and Chemical Research, Saitama, Japan.
Nakamura
T
T
Hasegawa
Y
Y
Miyamoto
K
K
Igarashi
M
M
Eto
Y
Y
Shibai
H
H
Titani
K
K
D028341:Activins; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003429:Cross Reactions; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000032:analysis* / Q000187:drug effects; D006801:Humans; D007265:Inhibins / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism
D011956:Receptors, Cell Surface; D028341:Activins; D007265:Inhibins; D005640:Follicle Stimulating Hormone