title: Polymorphonuclear leucocyte dysfunction during short term metabolic changes from normo- to hyperglycemia in type 1 (insulin dependent) diabetic patients.
abstract: Polymorphonuclear leucocyte (PMN) ingestion of particles coated with lipopolysaccharide (LPS) from Escherichia coli was compared to other PMN functions in seven patients with insulin dependent diabetes mellitus (IDDM) during short-term controlled metabolic changes from normo- to hyperglycemia without ketoacidosis. Factors known to interfere with PMN functions were excluded. PMN ingestion of particles coated with both LPS and bovine serum albumin became reduced from normo- to hyperglycemia. PMN motility was impaired in IDDM, but did not seem to be affected by short-term changes in metabolic control. PMN metabolism did not change from normo-to hyperglycemia. Particle-uptake by diabetic PMN is impaired after short term hyperglycemia in the range normally occurring in diabetics in every-day life.
authors.affiliation: Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Kjersem
authors.forename: J
authors.initials: J
authors.lastname: Hilsted
authors.forename: S
authors.initials: S
authors.lastname: Madsbad
authors.forename: J H
authors.initials: JH
authors.lastname: Wandall
authors.forename: K S
authors.initials: KS
authors.lastname: Johansen
authors.forename: N
authors.initials: N
authors.lastname: Borregaard
mesh_terms: D000328:Adult; D001770:Blood Bactericidal Activity; D001786:Blood Glucose / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology* / Q000378:metabolism; D004926:Escherichia coli; D006801:Humans; D006943:Hyperglycemia / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D008070:Lipopolysaccharides; D013481:Superoxides