Oncogene expression in reticuloendotheliosis virus-transformed lymphoid cell lines and avian tissues.
The genome of reticuloendotheliosis virus (REV-T) contains a unique oncogene, designated v-rel, which is inserted into the env region. Employing a cloned rel DNA probe, a single 2.9- to 3.0-kilobase v-rel mRNA was identified in poly(A)+ RNA from REV-T-transformed lymphoid cell lines. A 4.0-kilobase rel-specific transcript corresponding to the cellular homolog of the v-rel oncogene was identified in MSB-1 cells, a herpesvirus-transformed lymphoid cell line. Cytodot hybridization was used to quantitate the levels of rel, c-rel, c-myc, c-myb, c-abl, c-fms, c-Ha-ras, c-Ki-ras, c-src, c-yes, c-mos, and c-sis mRNA in REV-T-transformed cells. The levels of rel transcription in REV-T-transformed cells were elevated only two to eightfold over levels found in the transformed immature avian lymphoid cell line MSB-1. The relatively modest levels of rel transcription in REV-T-transformed cells and the significant differences between the lengths of the v-rel and c-rel mRNA suggest that REV-T transformation is the result of the production of an altered rel protein. The c-rel proto-oncogene is expressed in all avian hematopoietic tissues but is not expressed at significant levels in brain and muscle. The transcription of other proto-oncogenes is not enhanced in REV-T-transformed lymphoid cell lines.
Herzog
N K
NK
Bargmann
W J
WJ
Bose
H R
HR
D000818:Animals; D002060:Bursa of Fabricius / Q000032:analysis; D002472:Cell Transformation, Viral; D002645:Chickens; D005814:Genes, Viral; D006413:Hematopoietic System / Q000032:analysis; D008221:Lymphoid Tissue / Q000032:analysis; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D011061:Poly A / Q000096:biosynthesis*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D001356:Reticuloendotheliosis virus / Q000235:genetics* / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D014158:Transcription, Genetic
D009856:Oncogene Proteins, Viral; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A