title: TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein.
abstract: TnpA protein, the function encoded by the most abundant transcript of En-1 was expressed in Escherichia coli. DNA binding experiments with partially purified tnpA protein revealed that it binds to the subterminal repetitive region of En-1. TnpA protein recognizes a 12-bp-long sequence motif which is reiterated several times at the termini of En-1. Binding is reduced if the cytosine residues of CG dinucleotides and CNG trinucleotides within the motif are methylated. These data suggest a model in which the product of tnpA serves as a regulator of element activity.
authors.affiliation: Abteilung Molekulare Pflanzengenetik, Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
authors.forename: A
authors.initials: A
authors.lastname: Gierl
authors.forename: S
authors.initials: S
authors.lastname: Lütticke
authors.forename: H
authors.initials: H
authors.lastname: Saedler
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D010944:Plants / Q000235:genetics*; D003313:Zea mays / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D004247:DNA