title: Immobilization of Aspergillus ficuum phytase: product characterization of the bioreactor.
abstract: Aspergillus ficuum phytase was covalently immobilized on Fractogel TSK HW-75 containing 2-oxy-l-alkylpyridinium salts. A packed-bed bioreactor was constructed with the immobilized phytase. An HPLC ion-exchange method was used to analyze the enzymatic products of the bioreactor. Immobilized fungal phytase was able to hydrolyze myo-inositol Hexa-, penta-, tetra-, tri-, and diphosphates. When the substrate solution was recirculated for 5 hr in the bioreactor about 50% inorganic orthophosphate was released and myo-inositol-diphosphate and mono-phosphate were the only remaining products.
authors.affiliation: Southern Regional Research Laboratory ARS, USDA, New Orleans, Louisiana 70124.
authors.forename: A H
authors.initials: AH
authors.lastname: Ullah
authors.forename: B Q
authors.initials: BQ
authors.lastname: Phillippy
mesh_terms: D010832:6-Phytase / Q000302:isolation & purification* / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D004800:Enzymes, Immobilized / Q000302:isolation & purification*; D005285:Fermentation; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000096:biosynthesis
chemical_list: D004800:Enzymes, Immobilized; D007295:Inositol Phosphates; D010832:6-Phytase