Differential effects of 8-bromo-cyclic AMP on human chorionic gonadotropin (hCG), progesterone and estrogen production by term placental cells.
The present study examined the effects of a cAMP analog on the output of hCG and progesterone (P) in human term trophoblast cells in culture, as well as the conversion of androstenedione and testosterone to estradiol--17 beta and estrone by these cells. The levels of hCG and P in the culture medium increased throughout the four-day culture period. Addition of 8-bromo-adenosine-3',5'-monophosphate (8-Br-cAMP), forskolin or cholera toxin (but not 8-Br-cGMP) stimulated hCG and P production by the cultivated placental cells. In contrast, the presence of 8-Br-cAMP for 2 days significantly decreased basal estradiol-17 beta output, as well as conversion of androstenedione to estradiol-17 beta; the conversion of androstenedione to estrone was not affected. 8-Br-cAMP attenuated the conversion of testosterone to both estradiol-17 beta and estrone. These results further support the view that cAMP could have both stimulatory and inhibitory actions on placental hormonogenesis.
Rodway
M
M
Department of Obstetrics and Gynaecology, University of British Columbia, Grace Hospital, Vancouver, Canada.
Zhou
F Z
FZ
Benoit
J
J
Yuen
B H
BH
Leung
P C
PC
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000735:Androstenedione / Q000378:metabolism; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000502:physiology*; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000096:biosynthesis*; D005260:Female; D005865:Gestational Age; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000096:biosynthesis*; D013739:Testosterone / Q000378:metabolism
D006063:Chorionic Gonadotropin; D004967:Estrogens; D015124:8-Bromo Cyclic Adenosine Monophosphate; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D000242:Cyclic AMP