The role of hydroxyl radicals in the degradation of DNA by ozone.
The degradation of the nucleotides dAMP, dGMP, dCMP and dTMP and of calf thymus DNA by ozone was studied. In all cases both base and sugar moiety were degraded. Furthermore, strand breaks were induced in calf thymus DNA. Hydroxyl radicals were probably involved in the oxidation of the base in dAMP and of the deoxyribose ring, but not in the degradation of the other bases. This indicates that ozone-induced DNA damage proceeds both directly via ozone molecules and indirectly via hydroxyl radicals.
Van der Zee
J
J
Dept. Medical Biochemistry, Sylvius Laboratories, Leiden, The Netherlands.
Dubbelman
T M
TM
van Steveninck
J
J
D004249:DNA Damage; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D003843:Deoxycytidine Monophosphate / Q000378:metabolism; D003848:Deoxyguanine Nucleotides / Q000378:metabolism; D005609:Free Radicals; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D010126:Ozone / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013938:Thymidine Monophosphate / Q000378:metabolism
D003838:Deoxyadenine Nucleotides; D003848:Deoxyguanine Nucleotides; D005609:Free Radicals; D006878:Hydroxides; D003843:Deoxycytidine Monophosphate; D017665:Hydroxyl Radical; D013938:Thymidine Monophosphate; C012873:2'-deoxy-5'-adenosine monophosphate; D010126:Ozone; C007257:2'-deoxyguanosine 5'-phosphate