Glutathione specifically labeled with isotopes.
A procedure for synthesis of glutathione selectivity labeled with isotopes is described. A strain of Escherichia coli enriched in its content of gamma-glutamylcysteine synthetase and glutathione synthetase by recombinant DNA techniques is immobilized in a carrageenan matrix and treated with toluene to render the cells more permeable to the substrates. The immobilized cell matrix is incubated with a mixture containing the appropriately labeled amino acid, the other amino acid constituents of glutathione, ATP, and acetylphosphate. The radiolabeled product is isolated by column chromatography.
Murata
K
K
Abbott
W A
WA
Bridges
R J
RJ
Meister
A
A
D002250:Carbon Radioisotopes; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione; D005981:Glutathione Synthase / Q000235:genetics / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D010453:Peptide Synthases / Q000378:metabolism*; D014169:Transformation, Bacterial
D002250:Carbon Radioisotopes; D004274:DNA, Recombinant; D010453:Peptide Synthases; D005721:Glutamate-Cysteine Ligase; D005981:Glutathione Synthase; D005978:Glutathione