Induction of high affinity IL 2 receptors on B cells responding to anti-Ig and T cell-derived helper factors.
We have reported that IL 2 is one of the essential helper factors in culture supernatants from concanavalin A-activated spleen cells or T cell hybridomas that support proliferation and immunoglobulin secretion in B cell cultures responding to anti-immunoglobulin. Here we show that cells in such cultures consume IL 2 and bear high affinity IL 2 receptors detected by binding of purified, radiolabeled IL 2. Induction of high affinity IL 2 receptors depends on addition of both anti-immunoglobulin and helper factors, and does not occur in cultures given only anti-Ig, only helper factors, concanavalin A plus helper factors, or LPS. The majority of IL 2 receptors are on cells that also bear endogenous membrane immunoglobulin, because they are found in the membrane immunoglobulin-positive fraction when cultured cells are separated by fluorescence sorting after overnight culture in the absence of anti-immunoglobulin to allow reexpression of membrane immunoglobulin.
Prakash
S
S
Robb
R J
RJ
Stout
R D
RD
Parker
D C
DC
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000145:classification / Q000378:metabolism*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis / Q000096:biosynthesis*; D015375:Receptors, Interleukin-2; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D018800:Thy-1 Antigens; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D011947:Receptors, Antigen, B-Cell; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D018800:Thy-1 Antigens