title: Two linear plasmid-like DNA elements simultaneously maintained in Pleurotus ostreatus.
abstract: Two linear plasmid-like DNA elements, designated pLP01 and pLP02, have been isolated from a strain of Pleurotus ostreatus, an edible basidiomycete. pLP01 (10.0 kb) and pLP02 (9.4 kb) were found in mitochondrial preparations of the fungus and appear to have 5' ends blocked by association of a protein. Proteinase K cleavability of the 5'-terminal protein of pLP01 was higher than that of pLP02, indicating that the terminal proteins of both plasmid-like elements are distinct from one another. pLP01 and pLP02 were estimated to be present to the extent of 1-2 copies each per mitochondrial genome equivalent. The two plasmid-like elements had no homology between them and also were not homologous with the mitochondrial and nuclear genomic DNAs of the fungus.
authors.affiliation: Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yui
authors.forename: Y
authors.initials: Y
authors.lastname: Katayose
authors.forename: K
authors.initials: K
authors.lastname: Shishido
mesh_terms: D001487:Basidiomycota / Q000235:genetics*; D002499:Centrifugation, Density Gradient; D015342:DNA Probes; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005090:Exodeoxyribonucleases / Q000378:metabolism; D008928:Mitochondria / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011124:Polyporaceae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins
chemical_list: D015342:DNA Probes; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D014764:Viral Proteins; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; C043586:exo protein, Bacteriophage lambda; D004262:DNA Restriction Enzymes