title: The effect of human atrial natriuretic peptide on urinary sodium and urinary dopamine excretion in man.
abstract: Atrial extracts have long been known to produce natriuresis but it is only recently that atrial natriuretic peptide (ANP) itself has been isolated, purified and sequenced. We have now developed a specific radio-immunoassay for ANP and assessed its biological activity by infusing ANP in normal volunteers. Low dose ANP infusion (1.5 pmol/kg per min) produced no haemodynamic or natriuretic effects. High dose ANP infusion (15 pmol/kg per min) produced an increase in plasma immunoreactive ANP levels of 203 +/- 78 pmol/l and caused the urinary sodium excretion to increase from 3.5 +/- 1.6 to 11.0 +/- 7.4 mmol/30 min period (P < 0.05). No haemodynamic effects were seen with this high dose infusion. No changes in urinary dopamine were seen with either ANP infusion. This study shows that ANP is capable of producing a natriuresis in man in the absence of any generalized haemodynamic effects and that ANP may be an important modulator of salt and water balance in man.
authors.affiliation: Department of Clinical Pharmacology, Royal Postgraduate Medical School, London, UK.
authors.forename: A D
authors.initials: AD
authors.lastname: Struthers
authors.forename: J V
authors.initials: JV
authors.lastname: Anderson
authors.forename: R C
authors.initials: RC
authors.lastname: Causon
authors.forename: N D
authors.initials: ND
authors.lastname: Christofides
authors.forename: S R
authors.initials: SR
authors.lastname: Bloom
mesh_terms: D000328:Adult; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D004298:Dopamine / Q000652:urine*; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D008297:Male; D012964:Sodium / Q000652:urine*
chemical_list: D009320:Atrial Natriuretic Factor; D012964:Sodium; D004298:Dopamine