title: [Plasmids containing 2 new variants of the E.coli lacZ gene].
abstract: New plasmids containing partially deleted lacZ genes were obtained. These genes determine high-level synthesis of polypeptides of molecular mass 43-45 and 49-51 kD under the control of the lambda phage PR-promoter; inspite of the deletion, E. coli cells carrying new plasmids were found to possess beta-galactosidase activity. Use of these plasmids as new expression vectors is suggested.
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Khudiakov
authors.forename: T I
authors.initials: TI
authors.lastname: Kalinina
authors.forename: V S
authors.initials: VS
authors.lastname: Nepliueva
authors.forename: V D
authors.initials: VD
authors.lastname: Smirnov
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D004262:DNA Restriction Enzymes
vernacular_title: Plazmidy, soderzhashchie dva novykh varianta gena lacZ E. coli.