Evidence for multiple molecular weight forms of somatostatin-like material in Escherichia coli.
Extracts of Escherichia coli grown in defined medium contain somatostatin-related material (1-10 pg/g wet weight of cells). Preconditioned medium had no immunoactive somatostatin whereas, conditioned medium had 110-150 pg/l. Following purification of the extracted material on Sep-pak C18, Bio-Gel P-6 and HPLC, multiple molecular weight forms of somatostatin- (SRIF-) related material were identified. The material in one peak reacted in both the N-terminal and C-terminal SRIF immunoassay and coeluted on HPLC with SRIF-28, whereas that in a second peak eluted near SRIF-14 and was reactive only in the C-terminal SRIF assay. The two peaks are thus similar to SRIF-28 and SRIF-14 of vertebrates. These findings add support to the suggestion that vertebrate-type peptide hormones and neuropeptides have early evolutionary origins.
LeRoith
D
D
Pickens
W
W
Crosby
L K
LK
Berelowitz
M
M
Holtgrefe
M
M
Shiloach
J
J
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis*; D008970:Molecular Weight; D013004:Somatostatin / Q000302:isolation & purification*; D053561:Somatostatin-28
D003470:Culture Media; D013004:Somatostatin; D053561:Somatostatin-28