Serum from type IIA hyperlipoproteinemic patients does not stimulate proliferation of and collagen synthesis in human fetal aortic smooth muscle cells in culture.
The effect of serum from type IIA hyperlipoproteinemic patients on the proliferation and synthesis of collagen and other proteins of human fetal aortic smooth muscle cells (SMC) was studied. The activity of lysyl oxidase secreted into the culture medium was also measured. Type IIA hyperlipoproteinemic sera did not affect the proliferation of human aortic SMC as compared to normolipidemic sera, regardless of incubation time. The findings were similar for 3 different human fetal aortic SMC lines and one fibroblast line from adult human skin. The synthesis of collagen and other proteins was inhibited rather than stimulated in the presence of type IIA hyperlipoproteinemic sera. The activity of lysyl oxidase was not affected by type IIA hyperlipoproteinemic sera. The atherogenicity of hypercholesterolemia cannot be explained either by its direct effect on the proliferation of arterial SMC, as has been suggested by animal cell studies, or by its direct effect on the fibrogenicity of these cells.
Järveläinen
H
H
Halme
T
T
Lehtonen
A
A
Rönnemaa
T
T
D000328:Adult; D001011:Aorta; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005260:Female; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000097:blood*; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism; D012867:Skin
D009124:Muscle Proteins; D003094:Collagen; D008249:Protein-Lysine 6-Oxidase