Reduced DNA repair response of cells from carcinogen-induced rat liver altered foci exposed to chemical carcinogens in culture.
Cells were cultured from rat livers containing enzyme-altered foci induced by N-2-fluorenylacetamide and the level of DNA repair synthesis in response to several carcinogens was compared in gamma-glutamyl transpeptidase-positive foci cells and in enzyme-negative hepatocytes from the same livers. The level of unscheduled DNA synthesis elicited in foci cells by either the activation-dependent carcinogens N-2-fluorenylacetamide and diethylnitrosamine or the activation-independent carcinogens N-methyl-N'-nitro-N-nitrosoguanidine and 1-methyl-1-nitrosourea was significantly lower than that in hepatocytes. The results suggest that the repair of DNA damage by these altered cells is abnormal.
Mori
H
H
Sugie
S
S
Ohbayashi
F
F
Shima
H
H
Yoshimi
N
N
Takahashi
M
M
Williams
G M
GM
D015073:2-Acetylaminofluorene; D000818:Animals; D002273:Carcinogens; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008769:Methylnitronitrosoguanidine; D051381:Rats; D011912:Rats, Inbred ACI; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D015073:2-Acetylaminofluorene; D005723:gamma-Glutamyltransferase