title: Desaturation and chain elongation of essential fatty acids in isolated liver cells from rat and rainbow trout.
abstract: Isolated hepatocytes from rainbow trout and rat were incubated with 14C-labeled linoleic acid, linolenic acid, dihomogammalinolenic acid or eicosapentaenoic acid. The most striking difference in the desaturase activity was the lower level of delta 5 desaturase in trout than in rat. No delta 4 desaturation of 22:4(n-6) to 22:5(n-6) was observed in either of the two species, while the conversion of 22:5(n-3) to 22:6(n-3) was significant in both groups and highest in rainbow trout. The chain-elongating activity was remarkably similar in the two species, except for the "dead-end" elongation which was distinctly more important in fish.
authors.forename: T A
authors.initials: TA
authors.lastname: Hagve
authors.forename: B O
authors.initials: BO
authors.lastname: Christophersen
authors.forename: B H
authors.initials: BH
authors.lastname: Dannevig
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D004041:Dietary Fats; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005228:Fatty Acids, Essential / Q000378:metabolism*; D008042:Linolenic Acids / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014337:Trout; D017962:alpha-Linolenic Acid
chemical_list: D002250:Carbon Radioisotopes; D004041:Dietary Fats; D005228:Fatty Acids, Essential; D008042:Linolenic Acids; D010743:Phospholipids; D017962:alpha-Linolenic Acid; D015118:Eicosapentaenoic Acid; D044943:Fatty Acid Desaturases