title: Construction of a temperature-sensitive mutation for the direct identification of plasmids encoding DNA methyltransferases.
authors.affiliation: Medical Biotechnology Center, University of Maryland, Baltimore.
authors.forename: A
authors.initials: A
authors.lastname: Piekarowicz
authors.forename: R
authors.initials: R
authors.lastname: Yuan
authors.forename: D C
authors.initials: DC
authors.lastname: Stein
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D015254:DNA Modification Methylases / Q000235:genetics* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008745:Methylation; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D015254:DNA Modification Methylases; D004262:DNA Restriction Enzymes; C059678:mcrB protein, E coli