A new method for the determination in blood and urine of a novel triazolobenzodiazepine (estazolam) by HPLC.
In this study the authors report a new method to determine estazolam in blood and urine by high pressure liquid chromatography (HPLC). This compound is a triazolobenzodiazepine and shows an interesting psychopharmacological activity. The extraction from biological fluids was carried out using a mixture of ethylenechloride, methylenechloride, and ethylacetate (1:1:8). The HPLC analysis was carried out with a C18 column, using methanol:phosphate buffer (pH 7.5, 0.011M):acetonitrile (65:33:2) as mobile phase. The detector was set at lambda = 240 nm. The method shows good repeatability and a linear response in the range of 0.6 to 10 micrograms estazolam/mL both in serum and urine. A case of non-lethal acute intoxication in which the method was applied is also reported.
Scotto di Tella
A
A
Ricci
P
P
Di Nunzio
C
C
Cassandro
P
P
D000328:Adult; D014151:Anti-Anxiety Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004949:Estazolam / Q000032:analysis* / Q000097:blood / Q000506:poisoning / Q000652:urine; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D013405:Suicide
D014151:Anti-Anxiety Agents; D007202:Indicators and Reagents; D004949:Estazolam