title: Construction and application of plasmids containing bidirectional promoters of vaccinia virus.
abstract: A bidirectional expression vector containing both the 11KD late promoter (p11) and the presumptive 25KD early promoter (p25) was constructed. These bidirectional vectors have been applied to the expression of hepatitis B surface antigen by using one of the promoters for beta-galactosidase as the marker gene and the other one for hepatitis B surface antigen as the target gene.
authors.affiliation: Laboratory of Viral Genetics, Chinese Academy of Preventive Medicine, Beijing, People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Tsao
authors.forename: G Q
authors.initials: GQ
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Ruan
authors.forename: C M
authors.initials: CM
authors.lastname: Chu
mesh_terms: D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D005819:Genetic Markers; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014616:Vaccinia virus / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D005819:Genetic Markers; D006514:Hepatitis B Surface Antigens; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase