Partial purification of Chironex fleckeri (sea wasp) venom by immunochromatography with antivenom.
Chironex fleckeri crude venom was partially purified using immobilized commercially available ovoid antivenom. The antibody preparation reacted with lethal, hemolytic, dermonecrotic and mouse writhing (pain) factors in the crude venom. The lethal activity was purified five fold, while the specific eluate contained lower quantities of hemolytic, dermonecrotic and mouse writhing activities than did the crude venom.
Calton
G J
GJ
Burnett
J W
JW
D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D002642:Chick Embryo; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D003063:Cnidaria; D003064:Cnidarian Venoms / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D051381:Rats; D037401:Scyphozoa
D000997:Antivenins; D003064:Cnidarian Venoms