title: Hydrolysis of adenyl-5-yl imidodiphosphate by beef heart mitochondrial ATPase.
abstract: Beef heart mitochondrial ATPase (F1) catalyzes the hydrolysis of the ATP analog adenyl-5-yl imidodiphosphate (AMP-PNP). The reaction products are inorganic phosphate and adenyl-5-yl phosphoramidate (AMP-PN) as determined by HPLC analysis. The hydrolysis occurs in both the presence and absence of added divalent metal ions and is stimulated by potassium. The kinetic properties of the hydrolytic reaction depend markedly on the identity of the added divalent metal. GMP-PNP and AMP-CPP are also hydrolyzed, while AMP-PCP is not. Adenyl-5-yl phosphoramidate is a potent effect of beef heart mitochondrial ATPase activity. Based on these data, a reinterpretation of work based on the assumption that AMP-PNP is not hydrolyzed is presented.
authors.forename: T A
authors.initials: TA
authors.lastname: Tomaszek
authors.forename: S M
authors.initials: SM
authors.lastname: Schuster
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives*; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents; D002417:Cattle; D006868:Hydrolysis; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D000266:Adenylyl Imidodiphosphate; C012953:efrapeptin; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; D011188:Potassium