title: Loading rat liver mitochondria with apocytochrome c provokes exit of cytochrome c.
abstract: Rat liver mitochondria were loaded with cytochrome c by incubation with large amounts of [14C]apocytochrome c. After being washed they were incubated with either more apocytochrome c or cytochrome c. There was no release of labeled proteins from the mitochondria when incubated with cytochrome c. However, there was when incubated with apocytochrome c. The material released showed only one radioactive band which migrated as cytochrome c. Also no release of proteins other than cytochrome c was detected when liver mitochondria isolated from rats injected with [35S]methionine were incubated with apocytochrome c. These results suggest that the level and possibly the turnover of cytochrome c in rat liver mitochondria is regulated by the entry of apocytochrome c into mitochondria.
authors.affiliation: Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Spain.
authors.forename: V J
authors.initials: VJ
authors.lastname: Miralles
authors.forename: M J
authors.initials: MJ
authors.lastname: Marcote
authors.forename: J
authors.initials: J
authors.lastname: Hernández-Yago
authors.forename: S
authors.initials: S
authors.lastname: Grisolía
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000378:metabolism*; D001667:Binding, Competitive; D001692:Biological Transport; D003574:Cytochrome c Group / Q000378:metabolism*; D045304:Cytochromes c; D004792:Enzyme Precursors / Q000378:metabolism*; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
chemical_list: D001059:Apoproteins; D003574:Cytochrome c Group; D004792:Enzyme Precursors; D045304:Cytochromes c; D014357:Trypsin