Antileukemic effect of coral-prostanoids clavulones from the stolonifer Clavularia viridis on human myeloid leukemia (HL-60) cells.
To elucidate the biological activities of coral-prostanoids, clavulones, discovered from the Japanese stolonifer Clavularia viridis, we examined the effect of clavulone on the cell growth of human cancer (human promyelocytic leukemia (HL-60) cells and HeLa cells) and normal (Chang liver cells and lung fibroblasts) cells in vitro. Clavulone showed strong antiproliferative and cytotoxic activities in the human cells and it had some selectivity to leukemic (HL-60) cells over other HeLa cells or normal cells on the basis of the IC50 values and cytotoxic effect of the cells. The IC50 value of clavulone in the HL-60 cells was about 0.4 microM (0.2 micrograms/ml). Over 1.0 microM (0.5 micrograms/ml), clavulone showed a significant cytotoxic activity on the HL-60 cells. The data on DNA synthesis and flow cytometric analysis revealed that clavulone arrests the cells in the G1-phase and inhibits the cell growth of HL-60 cells by inhibiting S-phase DNA synthesis. These results suggest that clavulone has a potent antileukemic effect on HL-60 cells.
Honda
A
A
Yamamoto
Y
Y
Mori
Y
Y
Yamada
Y
Y
Kikuchi
H
H
D000970:Antineoplastic Agents; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003063:Cnidaria; D004261:DNA Replication / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy*; D008099:Liver / Q000166:cytology; D008168:Lung / Q000166:cytology; D011454:Prostaglandins A / Q000494:pharmacology*
D000970:Antineoplastic Agents; D011454:Prostaglandins A; C046249:clavulones