title: Structures of sugar chains of human kidney gamma-glutamyltranspeptidase.
abstract: gamma-Glutamyltranspeptidase purified from human kidneys contains 4-5 asparagine-linked sugar chains in each molecule. The sugar chains were released from the polypeptide portion of the enzyme by hydrazinolysis as oligosaccharides and separated by paper electrophoresis into one neutral and two acidic fractions. By sequential exoglycosidase digestion and methylation analysis, the neutral fraction, which comprised 69% of total oligosaccharides, was shown to be a mixture of bisected bi- and triantennary complex-type sugar chains with and without a fucose on the proximal N-acetylglucosamine residue and with Gal beta 1----4GlcNAc and/or Gal beta 1----4(Fuc alpha 1----3)GlcNAc groups in their outer chain moieties. The acidic oligosaccharide fractions were mixtures of mono- and disialyl derivatives of bisected triantennary complex-type oligosaccharides with Gal beta 1----4GlcNAc and/or Gal beta 1----4(Fuc alpha 1----3)GlcNAc group in their outer chain moieties. Some of the outer chains of the acidic oligosaccharides were considered to be sialylated X-antigenic structures.
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
authors.forename: A
authors.initials: A
authors.lastname: Hitoi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.forename: N
authors.initials: N
authors.lastname: Katunuma
authors.forename: A
authors.initials: A
authors.lastname: Kobata
mesh_terms: D002241:Carbohydrates / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D004590:Electrophoresis, Paper; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D009844:Oligosaccharides / Q000302:isolation & purification; D005723:gamma-Glutamyltransferase / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D009844:Oligosaccharides; D005723:gamma-Glutamyltransferase