title: Dynorphin peptides in human substantia nigra.
abstract: Stepwise processing of the prohormone for dynorphin-related peptides, proenkephalin B, may generate a large number of opioid peptides. It is therefore important to perform a chemical characterization of the immunoreactive dynorphin (ir-dyn) found in different tissues. In this study dynorphin peptides in human substantia nigra were characterized. Highly sensitive radioimmunoassays (RIA) directed against the C-terminals of dynorphin A (dyn A) and dynorphin B (dyn B) respectively, were used to measure the levels of dynorphin peptides. Tissue extraction was performed either with MeOH/0.1 M HCl (1/1) or 1 M HAc. Gel filtration on a Sephadex G-50 column revealed three peaks of ir-dyn B, the predominating one coeluting with synthetic dyn B. Ir-dyn A also appeared in three peaks, one of them (20-30%) coeluting with dyn A. The HAc extract contained much higher levels of ir-dyn B as compared to the MeOH/HCl extract, but approximately the same levels of ir-dyn A. Ion exchange separation of the HAc extract somewhat changed the size distribution pattern, with more ir coeluting with the synthetic peptides compared to other molecular weight forms.
authors.forename: I
authors.initials: I
authors.lastname: Christensson-Nylander
authors.forename: L
authors.initials: L
authors.lastname: Terenius
mesh_terms: D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004399:Dynorphins / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D011863:Radioimmunoassay; D013378:Substantia Nigra / Q000032:analysis*
chemical_list: D004399:Dynorphins