title: Influence of ligation of pancreatic and bile ducts on the interaction of vasoactive intestinal peptide with rat duodenal epithelial cells.
abstract: The specific binding of vasoactive intestinal peptide (VIP) and the stimulatory effect of the neuropeptide on cyclic AMP in duodenal epithelial cells were modified 3 days following pancreaticobiliary exclusion. The binding capacity, but not the affinity, of VIP receptors decreased (by about 50%) as a consequence of the surgical manipulation. VIP was equally potent but showed a lower efficiency (about 45%) in stimulating cyclic AMP after ligation of the pancreatic and bile ducts. These observations may be either a consequence or a cause of the adaptive response of duodenal epithelium, the last possibility suggesting a role of VIP in the mechanisms of growth and differentiation of intestinal mucosa.
authors.forename: J L
authors.initials: JL
authors.lastname: Díaz-Juárez
authors.forename: E
authors.initials: E
authors.lastname: Arilla
authors.forename: J C
authors.initials: JC
authors.lastname: Prieto
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000502:physiology; D001667:Binding, Competitive; D004386:Duodenum / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005704:Gallbladder / Q000502:physiology*; D007700:Kinetics; D008297:Male; D010179:Pancreas / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide