title: Chemicals influencing maturation, activation, and degeneration of bovine oocytes in culture.
abstract: The effects of the dibutyryl cyclic adenosine 3',5'-monophosphate, calmodulin antagonist W7, and ovarian glycosaminoglycans on polar body extrusion, "nucleus" formation, and segmentation of bovine oocytes were examined in vitro. Dibutyryl cyclic adenosine 3',5'-monophosphate (100 microM) accelerated the extrusion of first polar body; however, W7 (20 microM) suppressed the formation of the first polar body. Spontaneous "nucleus" formation resembling pronucleus formation in oocytes was accelerated in the presence of dibutyryl cyclic adenosine 3',5'-monophosphate at concentrations of greater than 20 microM. The spontaneous segmentation, which is a degenerative change, was suppressed in the presence of bovine follicular fluid-glycosaminoglycans at concentrations of 250 micrograms/ml or greater. These results may indicate that maturation, activation, and degeneration of bovine oocytes in culture are modulated by dibutyryl cyclic adenosine 3',5'-monophosphate, W7, and ovarian glycosaminoglycans.
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Sato
authors.forename: N
authors.initials: N
authors.lastname: Kawamura
authors.forename: T
authors.initials: T
authors.lastname: Ishibashi
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000502:physiology; D003994:Bucladesine / Q000494:pharmacology*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D006025:Glycosaminoglycans / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D006080:Ovarian Follicle / Q000502:physiology; D010455:Peptides / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*
chemical_list: D002147:Calmodulin; D006025:Glycosaminoglycans; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D013449:Sulfonamides; C018355:intraovarian peptides; D003994:Bucladesine; C017967:W 7