Purification and crystallization of thymidine kinase from herpes simplex virus type 1.
Thymidine kinase from herpes simplex virus type 1 (ATP:thymidine 5'-phosphotransferase; EC 2.7.1.21) has been purified from an overexpression system and crystallized against ammonium sulfate by using the hanging-drop technique. The tetragonal crystals are of space group P4122 or P4322, and have unit cell dimensions a = b = 84 A, c = 180 A.
Sanderson
M R
MR
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CN 06511.
Freemont
P S
PS
Murthy
H M
HM
Krane
J F
JF
Summers
W C
WC
Steitz
T A
TA
D003460:Crystallization; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000302:isolation & purification; D014961:X-Ray Diffraction
D013937:Thymidine Kinase