Simultaneous determination of vitamin K1, vitamin K1 2,3-epoxide and menaquinone-4 in human plasma by high-performance liquid chromatography with fluorimetric detection.
A highly sensitive method for measuring endogenous vitamin K1, menaquinone-4 (which is one of the K2 vitamins) and vitamin K1 2,3-epoxide in human plasma was developed, based on high-performance liquid chromatography with coulometric reduction and fluorimetric detection, following extraction from plasma and purification on a Sep-Pak silica cartridge. The detection limits of vitamin K1, menaquinone-4 and vitamin K1 2,3-epoxide were 5, 5 and 8 pg per injection for the standard substances and 30, 30 and 50 pg/ml in human plasma, respectively.
Hirauchi
K
K
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Sakano
T
T
Nagaoka
T
T
Morimoto
A
A
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D010472:Perchlorates; D012737:Sex Factors; D017670:Sodium Compounds; D013050:Spectrometry, Fluorescence; D014812:Vitamin K / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D010837:Vitamin K 1 / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine; D024482:Vitamin K 2 / Q000031:analogs & derivatives
D010472:Perchlorates; D017670:Sodium Compounds; D024482:Vitamin K 2; D014812:Vitamin K; C016186:vitamin K1 oxide; C030814:menatetrenone; D010837:Vitamin K 1; C031068:sodium perchlorate