title: Tn916 delta E: a Tn916 transposon derivative expressing erythromycin resistance.
abstract: The tetracycline resistance gene encoded within the transposon Tn916 was replaced with the gene encoding erythromycin resistance from the plasmid pVA838. The derivative transposon of Tn916 was designated Tn916 delta E and was introduced into the Streptococcus faecalis chromosome by protoplast transformation. The conjugation/transposition functions of Tn916 delta E were similar to those observed for Tn916 in S. faecalis and Tn916 delta E was capable of self-conjugation at frequencies similar to those of other S. faecalis and Group B Streptococcus. This transposon will be useful for mutagenesis studies in gram-positive organisms, especially in those species where erythromycin resistance is a more desirable selectable marker.
authors.affiliation: Department of Pediatrics, University of Washington, Seattle.
authors.forename: C E
authors.initials: CE
authors.lastname: Rubens
authors.forename: L M
authors.initials: LM
authors.lastname: Heggen
mesh_terms: D015139:Blotting, Southern; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D011815:R Factors
chemical_list: D004251:DNA Transposable Elements; D004917:Erythromycin