Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe.
A DNA probe to detect genes conferring localized adherence of enteropathogenic Escherichia coli (EPEC) to Hep-2 cells was evaluated by using E. coli isolates from the stools of Peruvian infants with and without diarrhea. The probe was both sensitive and specific and revealed that Hep-2 adherence (because of the EPEC adherence factor [EAF] was more frequent in some O serogroups of EPEC than in others. Those serogroups in which EAF is almost always found have been designated class I EPEC; serogroups in which EAF is rarely found have been designated class II. Both class I (EAF-positive) and class II EPEC are associated with diarrheal disease.
Nataro
J P
JP
Baldini
M M
MM
Kaper
J B
JB
Black
R E
RE
Bravo
N
N
Levine
M M
MM
D018830:Adhesins, Escherichia coli; D000268:Adhesiveness; D001426:Bacterial Proteins / Q000235:genetics*; D002460:Cell Line; D004269:DNA, Bacterial; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D007223:Infant; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012703:Serotyping
D018830:Adhesins, Escherichia coli; D001426:Bacterial Proteins; D004269:DNA, Bacterial