title: Antibody to cloned HSV glycoproteins B and D plus adult human leukocytes protect neonatal mice from lethal HSV infection.
abstract: Antisera produced by HSV infection or following vaccination of guinea pigs with the cloned herpes simplex virus (HSV) glycoproteins gB and gD were compared for in vitro antibody-dependent cellular cytotoxicity (ADCC) activity and for in vivo protection. Antibody from guinea pigs was able to participate in ADCC with human mononuclear cells in vitro, anti-gBgD serum being equivalent to HSV convalescent sera. In vivo, each of the guinea pig sera was able to protect neonatal mice from a fatal HSV-1 infection when given with human mononuclear cells but not when given alone. The anti-gBgD serum was the most effective in vivo, protecting 15 of 17 (88%) neonatal mice when given at a 10(-4) dilution with human mononuclear cells and was the only guinea pig serum protective at a 10(-6) dilution (5 of 7 neonatal mice).
authors.affiliation: James N. Gamble Institute of Medical Research, Cincinnati, OH 45219.
authors.forename: D I
authors.initials: DI
authors.lastname: Bernstein
authors.forename: L S
authors.initials: LS
authors.lastname: Loo
authors.forename: S
authors.initials: S
authors.lastname: Kohl
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000096:biosynthesis; D000920:Antibody-Dependent Cell Cytotoxicity; D005260:Female; D006168:Guinea Pigs; D006561:Herpes Simplex / Q000517:prevention & control*; D006801:Humans; D007116:Immunization, Passive; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018139:Simplexvirus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C039447:glycoprotein D, Human herpesvirus 1