DNA conformational variations in the in vitro torsionally strained Ig kappa light chain gene localize on consensus sequences.
We have analyzed the localization and the dependence upon superhelical density of the DNA sites which modify their conformation under torsional strain in a mouse Ig L kappa gene. The conformational variations occur on DNA sites which have been defined as protein interaction sites and consensus sequence motifs: the 5'-upstream regulatory decanucleotides, the TATA sequence, the consensus heptanucleotides of the J recombinational sequences.
Venditti
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Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, Italy.
Caserta
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Di Mauro
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Camilloni
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G
D000818:Animals; D001426:Bacterial Proteins; D001483:Base Sequence; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004274:DNA, Recombinant / Q000378:metabolism; D004278:DNA, Superhelical / Q000187:drug effects / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009993:Osmium Tetroxide / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D004278:DNA, Superhelical; D007145:Immunoglobulin kappa-Chains; D004247:DNA; D004720:Endonucleases; C025580:endodeoxyribonuclease XBAI; C024404:BglII endonuclease; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D015719:Single-Strand Specific DNA and RNA Endonucleases; D009993:Osmium Tetroxide