Synthesis of glycoproteins of syncytial and non-syncytial strains of herpes simplex virus type 1 and their transport to the plasma membrane.
Underglycosylated form of glycoprotein C (gC) synthesized in the presence of tunicamycin was found on the surface membrane of BHK cells infected with the non-syncytial (non-syn) strain Kupka of herpes simplex virus type 1 (HSV-1). Such form of gC was not detected in the plasma membrane of tunicamycin-treated cells infected with the syncytial (syn) HSZP strain of HSV-1. In comparison to the non-syn strain Kupka, a smaller amount of glycoproteins of the syn HSZP strain was detected on the surface membrane of Vero cells. The same results were obtained using BHK cells, in which no polykaryocytes were induced. The individual glycoproteins of both strains under study had been synthesized and accumulated in a similar way in infected cells. However, at least in accumulated in a similar way in infected cells. However, at least in Vero cells a delay in the processing of syn strain HSZP glycoproteins was observed.
Raucina
J
J
Bystrická
M
M
Matis
J
J
Lesso
J
J
D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006224:Cricetinae; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D018139:Simplexvirus / Q000145:classification / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology; D014764:Viral Proteins / Q000378:metabolism*
D006023:Glycoproteins; D008565:Membrane Proteins; D014764:Viral Proteins; D014415:Tunicamycin