A transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair.
The formation of single-stranded breaks in DNA following UV irradiation is assessed in uvrC34 mutants. By altering the SOS DNA-repair system, either by additional mutations or by using drugs affecting transcription or translation, it is shown that such single-stranded breaks require one or more DNA-damage-inducible functions. A UV-sensitive strain is characterized as carrying a Tn10 insertion into the uvrC gene. The absence of post-irradiation incision in this strain demonstrates that uvrC function is absolutely required in vivo for the incision stage of excision repair, and suggests that other uvrC mutants are 'leaky'.
Walters
R G
RG
Department of Genetics, Liverpool University, UK.
Wilbraham
H O
HO
Strike
P
P
Forster
J W
JW
D001426:Bacterial Proteins; D004260:DNA Repair; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D013014:SOS Response, Genetics; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial