Immunochemical recognition of oxytocin by antiserum to L-prolyl-leucyl-glycine amide.
Antisera were raised in rabbits against Pro-Leu-Gly-NH2 (PLG)-bovine thyroglobulin conjugates. Specificity of the antisera towards oxytocin was evaluated by a radioimmunoassay as well as by immunohistochemical procedures on hypothalamic tissue and hormone bound to Sepharose beads. It was concluded that immunization with PLG can raise antisera that are specific to oxytocin.
Watkins
W B
WB
Peek
J C
JC
D000818:Animals; D000918:Antibody Specificity; D001001:Anura; D002645:Chickens; D005455:Fluorescent Antibody Technique; D006054:Goldfish; D007031:Hypothalamus / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D009075:MSH Release-Inhibiting Hormone / Q000276:immunology*; D010121:Oxytocin / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats
D010121:Oxytocin; D009075:MSH Release-Inhibiting Hormone