Transforming genes in duck hepatic carcinoma--mht(raf) and Ha-ras.
The transfection of NIH 3T3 cells was performed with DNAs from 2 duck primary hepatic carcinomas (DHC 40K, 9K) and 1 tumor-adjacent liver tissue (TAL, 9N). Transfectants were found from 40K, 9K and 9N DNAs. The secondary transfectants were obtained after transfection of RAT-1 cells with DNAs from primary transfectants. After hybridization with Ha-ras, Ki-ras, N-ras and mht oncogenes, it was found that duck mht (5.2 and 3.2 kb EcoRI fragments) and duck Ha-ras (3.4 kb EcoRI fragments) were present in all these transformants. This is the first report on transforming genes in duck primary hepatic cancer as well as tumor-adjacent liver tissue.
Tan
H
H
Department of Biochemistry & Molecular Biology, Shanghai Cancer Institute.
Liu
C T
CT
Hong
W N
WN
Tang
G Y
GY
Zhang
S Y
SY
Lu
Z X
ZX
Chen
Y Q
YQ
Jiang
H Q
HQ
Gu
J R
JR
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000662:veterinary*; D004273:DNA, Neoplasm / Q000235:genetics; D004372:Ducks; D011905:Genes, ras; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics / Q000662:veterinary*; D051379:Mice; D009857:Oncogenes; D011201:Poultry Diseases / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D004273:DNA, Neoplasm