title: Vertical transmission of channel catfish virus.
abstract: Channel catfish broodfish were examined nondestructively for the presence of channel catfish virus (CCV), using a nucleic acid-probing technique. A dot blot assay was tested and shown to be accurate and rapid in the diagnosis of CCV. Two CCV-positive fish were successfully mated, and fertilized eggs were collected. Offspring that hatched were tested and were shown to have CCV. This result indicated vertical transmission of CCV, a herpesvirus.
authors.affiliation: Department of Biochemistry, Mississippi State University 39762.
authors.forename: J A
authors.initials: JA
authors.lastname: Wise
authors.forename: S F
authors.initials: SF
authors.lastname: Harrell
authors.forename: R L
authors.initials: RL
authors.lastname: Busch
authors.forename: J A
authors.initials: JA
authors.lastname: Boyle
mesh_terms: D000818:Animals; D001345:Autoradiography; D002397:Catfishes / Q000382:microbiology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005393:Fish Diseases / Q000175:diagnosis / Q000382:microbiology / Q000635:transmission*; D006564:Herpesviridae / Q000032:analysis; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000635:transmission / Q000662:veterinary*; D007059:Ictaluridae / Q000382:microbiology*; D008297:Male
chemical_list: D015342:DNA Probes; D004279:DNA, Viral