Effect of glucocorticoid administration on intestinal, renal, and cerebellar calbindin-D28K in chicks.
A radioimmunoassay for chick intestinal calcium-binding protein (calbindin-D28K, CaBP-28K) has been developed in our laboratory with a detection limit of 0.3 ng/ml. The values for CaBP-28K in vitamin D-deficient (-D) chicks ranged from a high value for the cerebellum (21,400 +/- 580 ng/mg protein) to a scarcely detectable level in the liver (19.6 +/- 2.2 ng/mg protein). After administration of vitamin D (vitamin D3 500 IU p.o. for 7 days) (+D), the levels of CaBP-28K increased in the duodenum (52,300 +/- 5,100 ng/mg protein), ileum (45,200 +/- 740 ng/mg protein), cerebellum (22,000 +/- 470 ng/mg protein), colon (15,200 +/- 330 ng/mg protein), and kidney (13,460 +/- 540 ng/mg protein). However, the increment in the level of CaBP-28K in each tissue after vitamin D administration was different; levels of CaBP-28K in the duodenum, ileum, and colon increased dramatically more than 200 times after vitamin D administration, whereas that in the kidney showed only a 2.5-fold increase and was unaltered in the cerebellum.(ABSTRACT TRUNCATED AT 250 WORDS)
Tohmon
M
M
Department of Medicine, Kobe University, School of Medicine, Japan.
Fukase
M
M
Kishihara
M
M
Kadowaki
S
S
Fujita
T
T
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D064026:Calbindins; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D002645:Chickens; D002762:Cholecalciferol / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D012016:Reference Values; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D014808:Vitamin D Deficiency / Q000378:metabolism
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D002762:Cholecalciferol; D003907:Dexamethasone