Oxygen-dependent killing of Staphylococcus aureus by human neutrophils.
Luminol-dependent chemiluminescence was used as a monitor of reactive oxidant generation during phagocytosis of Staphylococcus aureus by human neutrophils. Reactive oxidants play a crucial role in the killing of this organism because: (a) S. aureus was killed most rapidly when the rate of increase of chemiluminescence was greatest; (b) neutrophils which had been activated to generate reactive oxidants by re-aeration of anaerobic suspensions killed this bacterium more efficiently than control suspensions; and (c) neutrophils from a patient with chronic granulomatous disease could neither generate reactive oxidants nor kill S. aureus.
Edwards
S W
SW
Department of Biochemistry, University of Liverpool, UK.
Say
J E
JE
Hart
C A
CA
D000332:Aerobiosis; D000693:Anaerobiosis; D001770:Blood Bactericidal Activity; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D013481:Superoxides; D010100:Oxygen