Cyclic GMP, cyclic AMP, glucose at birth, and maturation of rat liver mitochondria.
The improvement in mitochondrial functions which normally occurs in newborn rat liver in vivo during the few hours following delivery is inhibited by a glucose injection at birth (Meister, R., Comte, J., Baggetto, L., G., Godinot, C. and Gautheron, D.C. (1983) Biochim. Biophys. Acta 722, 36-42). To test whether this improvement could be correlated to changes in cyclic nucleotides, the levels of cAMP and cGMP have been measured during the 2 h following birth. At birth, a short rise followed by a decrease of cAMP occurs, then a significant increase of cAMP level is observed between 45 min and 2 h. The cAMP level for animals injected at birth with glucose is lower than for control animals at each time studied. The cGMP level is not significantly affected in control animals, while in glucose-treated animals a significant decrease of cGMP is observed in the postnatal 2 h. The present work shows also that the glucose-induced inhibition of mitochondrial maturation is mimicked by injection at birth of either 8-Br-cGMP or nitroprusside. The latter transiently increases intracellular cGMP. In contrast, the glucose-induced inhibition is prevented by the injection at birth of either dbcAMP or alkylxanthines together with glucose (Comte, J., Meister, R., Baggetto, L.G., Godinot, C. and Gautheron, D.C. (1986) Biochem. Pharmacol. 35, 2411-2416). It is concluded that the postnatal improvement of mitochondrial functions is stimulated by cAMP and inhibited by cGMP, and that glucose-induced inhibition of the maturation is at least partly supported by a decrease in cAMP but not correlated to an increase in cGMP.
Meister
R
R
Laboratoire de Biologie et Technologie des Membranes C.N.R.S., Université Claude Bernard, Villeurbanne, France.
Comte
J
J
Godinot
C
C
Gautheron
D C
DC
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000254:growth & development*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000502:physiology*; D009599:Nitroprusside / Q000494:pharmacology; D010084:Oxidation-Reduction; D010431:Pentoxifylline / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009599:Nitroprusside; C016276:8-bromocyclic GMP; D000244:Adenosine Diphosphate; D003994:Bucladesine; D000242:Cyclic AMP; D006152:Cyclic GMP; D005947:Glucose; D010431:Pentoxifylline