The synthesis of human alpha-2-HS glycoprotein is down-regulated by cytokines in hepatoma HepG2 cells.
The regulation of the synthesis of alpha-2-HS glycoprotein (AHSG) by inflammatory mediators from activated monocytes was studied on the human hepatoma cell line HepG2 and compared to that of albumin. Monocyte-conditioned medium, recombinant human interleukin-6 (rhIL6) and interleukin-1 beta (rhIL1 beta) all down-regulated the synthesis of AHSG. This decrease was found both at the protein and the mRNA level. The most efficient mediator was the monocyte-conditioned medium, when rhIL1 beta was found to be less efficient than rhIL6. The combination of rhIL6 and rhIL1 beta resulted in an additive down-regulation of the AHSG mRNA levels. Similar results were obtained with albumin. These data indicate that AHSG is a negative acute-phase protein whose synthesis is regulated by cytokines in a manner similar to that of albumin.
Daveau
M
M
INSERM Unité 78, Bois-Guillaume, France.
Christian-Davrinche
Julen
N
N
Hiron
M
M
Arnaud
P
P
Lebreton
J P
JP
D001798:Blood Proteins / Q000096:biosynthesis* / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D015847:Interleukin-4; D015850:Interleukin-6; D007378:Interleukins / Q000502:physiology*; D008113:Liver Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology*; D060749:alpha-2-HS-Glycoprotein
C556354:AHSG protein, human; D001798:Blood Proteins; D007370:Interferon Type I; D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D060749:alpha-2-HS-Glycoprotein; D015847:Interleukin-4