Mineral structure and preferred orientation in the fin bones of the plaice, Pleuronectes platessa.
The technique of collagenase etching of a polished surface of plaice fin bone has been used to reveal the structural detail of the mineral component and hence to explain the preferred orientation previously deduced from X-ray diffraction experiments. High resolution electron micrographs reveal units approximately 20 nm across, which aggregate to form units approximately 100 nm across, which in turn coalesce to produce rods up to approximately 1 micron diameter and of substantial length. These rods show a preferred orientation with their axes parallel to the long axis of the bone, thus demonstrating a preferred orientation of the mineral component. Additionally, collagenase-etched transverse surfaces reveal numbers holes from which collagen fibrils parallel to the long axis of the bone have been removed, whereas similarly treated longitudinal surfaces do not show such holes. This is consistent with a predominance of collagen fibrils running along the bone axis and explains the previously observed preferred orientation of the collagen component of fish fin bones.
Green
M
M
Department of Materials Engineering, University College of Swansea, Singleton Park, UK.
Isaac
D H
DH
Jenkins
G M
GM
D000818:Animals; D001842:Bone and Bones / Q000648:ultrastructure*; D005412:Flatfishes / Q000033:anatomy & histology*; D003012:Microbial Collagenase; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000032:analysis
D008903:Minerals; D003012:Microbial Collagenase