title: Highly sensitive and reliable chemiluminescence method for the assay of superoxide dismutase in human erythrocytes.
abstract: Superoxide dismutase in human erythrocytes was assayed by the inhibition of highly diluted erythrocyte lysates on 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazol[1,2-alpha]pyra zin-3-one- dependent luminescence induced by the hypoxanthine-xanthine oxidase system. Our chemiluminescence procedure gave 95-times higher sensitivity than the cytochrome c method. The concentration of superoxide dismutase in erythrocytes of Down's syndrome patients was approx. 1.8-times higher than that of normal humans.
authors.affiliation: College of Medical Care and Technology, Gunma University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Nakano
mesh_terms: D003574:Cytochrome c Group; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D049449:Luminescence; D008722:Methods; D013053:Spectrophotometry / Q000379:methods; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D003574:Cytochrome c Group; D013482:Superoxide Dismutase