Rhodamine 6G inhibits the matrix-catalyzed processing of precursors of rat-liver mitochondrial proteins.
Several inner membrane proteins from rat liver mitochondria have been translated for the first time in rabbit reticulocyte lysates. These include the Rieske iron-sulfur protein, cytochrome c1 and core protein I of the cytochrome bc1 complex, the alpha and beta subunits of F1 ATPase, and subunit IV of cytochrome oxidase. All were translated from free polysomes as larger-molecular-mass precursors, and were processed to their mature forms by isolated liver mitochondria or by the isolated mitochondrial matrix fraction. In vitro processing, catalyzed by the isolated matrix fraction, is inhibited by rhodamine 6G. The latter is a fluorescent probe, which accumulates specifically in mitochondria of whole cells and which is used extensively to visualize mitochondrial morphology. The concentration of rhodamine 6G required for inhibition in vitro is similar to that of o-phenanthroline. Rhodamine 6G inhibits matrix-catalyzed processing of all precursors tested, indicating that the mechanism of inhibition is common for a variety of functionally unrelated precursors. The novel action of rhodamine 6G reported here can form the basis for its inhibition of precursor processing in intact hepatoma cells [Kolarov, J. & Nelson, B.D. (1984) Eur. J. Biochem. 144, 387-392].
Kuzela
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D000818:Animals; D002474:Cell-Free System; D003575:Cytochromes c1 / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D051381:Rats; D012235:Rhodamines / Q000494:pharmacology*; D014966:Xanthenes / Q000494:pharmacology*
D007506:Iron-Sulfur Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D011498:Protein Precursors; D012235:Rhodamines; D014966:Xanthenes; C026188:rhodamine 6G; D003575:Cytochromes c1; D003576:Electron Transport Complex IV; D006180:Proton-Translocating ATPases