title: Movements of protons coupled to glucose transport in yeasts. A comparative study among 248 yeast strains.
abstract: In 248 strains representing 205 yeast species, changes in pH coupled to glucose addition were followed in unbuffered cell suspensions. Alkalinization of the external medium elicited by glucose, indicating a H+-glucose symport was observed in 34% of the strains, most of them belonging to the genera Rhodotorula, Hansenula and Candida. H+ uptake coupled to glucose transport was observed only after exhaustion of glucose in growth media. This observation was taken as an indication that, in general, the synthesis of H+-glucose symport is under the control of catabolite repression. Subsequently to the addition of glucose, in most yeasts (82%) acidification was observed. This ability is probably related to the creation of a proton-gradient across the plasma membrane and is generally distributed among yeasts.
authors.affiliation: Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, Portugal.
authors.forename: M C
authors.initials: MC
authors.lastname: Loureiro-Dias
mesh_terms: D001692:Biological Transport; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011522:Protons; D015003:Yeasts / Q000378:metabolism*
chemical_list: D011522:Protons; D005947:Glucose