The effects of temperature on the cytochrome P-450 system of thermally acclimated bluegill.
The effects of temperature acclimation at 10, 20 and 30 degrees C on the concentration and activity of the mixed function oxidase system in bluegill are as follows. Liver weight/body weight varied inversely with temperature. Significant (P less than 0.05) differences in concentration of cytochrome P-450 of hepatic microsomes were seen and varied inversely with temperature. Benzo(a)pyrene hydroxylase activity tested in vitro at incubation temperatures of 10, 20 and 30 degrees C showed significant differences in Km, but no differences in Vmax. SDS polyacrylamide gel electrophoresis revealed some quantitative differences in cytochrome P-450 isozymes between groups.
Karr
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Reinert
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Wade
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D000064:Acclimatization; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D001835:Body Weight; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005399:Fishes / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology / Q000502:physiology; D008862:Microsomes, Liver / Q000201:enzymology*; D009929:Organ Size; D013696:Temperature
D003577:Cytochrome P-450 Enzyme System; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases