Variation among acetylcholine receptor clusters induced by ciliary ganglion neurons in vitro.
We have examined the variation in receptor density and area among neurite-associated acetylcholine receptor patches (NARPs) induced by chick ciliary ganglion neurons on nearby myotubes in vitro. Quantitative analysis of rhodamine-alpha-bungarotoxin (RBTX) NARPs revealed that about 15% of the NARPs were "outstanding" in terms of size (greater than 60 micron 2) and fluorescence intensity (greater than 100 units on a 0-255 scale). The total number of receptors at different NARPs ranged over 3 orders of magnitude. It is likely that variation in NARP size and intensity reflects regional variation in the ability of myotubes to respond to the neuronal influence because (1) no gradient in NARP size or intensity with distance from the soma was evident; (2) the intensities and areas of uninnervated receptor clusters (hot spots) were similar to those of NARPs; (3) acetylcholinesterase was present at the same proportion of hot spots and NARPs at all times examined. We found no physiological or morphological evidence that outstanding NARPs were more effective sites of transmitter release. Outstanding NARPs were restricted to the longest neurite of individual neurons, so they may signal trophic interactions of the sort that promote neurite outgrowth and survival.
Dubinsky
J M
JM
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Morgan
M
M
Fischbach
G D
GD
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D002642:Chick Embryo; D004627:Embryonic Induction; D005726:Ganglia, Parasympathetic / Q000502:physiology* / Q000648:ultrastructure; D066298:In Vitro Techniques; D007546:Isoquinolines; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009469:Neuromuscular Junction / Q000502:physiology*; D011978:Receptors, Nicotinic / Q000502:physiology*; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission
D002038:Bungarotoxins; D007546:Isoquinolines; D011978:Receptors, Nicotinic; C017475:lucifer yellow; D000110:Acetylcholinesterase