The use of micropeptide maps and monoclonal antibodies for antigenic variation analysis of influenza A viruses isolated in China from 1972 to 1983.
125I-labelled haemagglutinin (HA) micropeptide mapping and haemagglutinin inhibition (HAI) tests with monoclonal antibodies were used in analysing antigenic variation of six strains of influenza A virus isolated from 1972 to 1983 in China. The results from micropeptide mapping generally coincided with those of sequence analysis. 125I-labelled HA micropeptide mapping is a simple, reproducible and practical method for surveillance of influenza, especially in combination with HAI test using monoclonal antibodies.
Wang
M L
ML
Yan
J X
JX
Lin
Q A
QA
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002642:Chick Embryo; D002681:China; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000032:analysis* / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000145:classification / Q000276:immunology*; D010455:Peptides / Q000032:analysis
D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D010455:Peptides