title: Interleukin-1 affects the function of cultured human thyroid cells.
abstract: Cytokines are peptide hormones essential for cellular communication in the immune response. The purpose of this study was to investigate the influence of cytokines, especially recombinant interleukin 1 beta (rIL-1 beta), on human thyroid cells. Thyroglobulin (Tg) was measured by a double antibody radioimmunoassay, and cyclic AMP (cAMP) by a competitive protein binding assay. Supernatants from unstimulated and phytohaemagglutinin-stimulated blood mononuclear cells were added to human thyroid cells cultured in monolayers. A dose-dependent inhibition of the secretion of Tg and cAMP was demonstrated. Both subcultured and primary cultured cells incubated with rIL-1 beta at pharmacological levels (10(-1)-10(2) U/ml) exhibited an inhibition of Tg and cAMP secretion, while at physiological levels (10(-5)-10(-3) U/ml), the secretion of Tg was enhanced. The similar stimulation of cAMP was demonstrated in subcultures. These in vitro studies suggest that IL-1 beta may play a role in the pathogenesis of autoimmune thyroid diseases. Further, the stimulations at low concentrations indicate that IL-1 beta may regulate the function of the thyroid gland under physiological conditions.
authors.affiliation: Medical Dept. of Endocrinology, Frederiksberg Hospital, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Krogh Rasmussen
authors.forename: K
authors.initials: K
authors.lastname: Bech
authors.forename: U
authors.initials: U
authors.lastname: Feldt-Rasmussen
authors.forename: S
authors.initials: S
authors.lastname: Poulsen
authors.forename: I
authors.initials: I
authors.lastname: Holten
authors.forename: M
authors.initials: M
authors.lastname: Ryberg
authors.forename: C A
authors.initials: CA
authors.lastname: Dinarello
authors.forename: K
authors.initials: K
authors.lastname: Siersbaek-Nielsen
authors.forename: T
authors.initials: T
authors.lastname: Friis
authors.forename: K
authors.initials: K
authors.lastname: Bendtzen
mesh_terms: D001327:Autoimmune Diseases / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000494:pharmacology* / Q000502:physiology; D013954:Thyroglobulin / Q000378:metabolism*; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D013972:Thyrotropin; D013954:Thyroglobulin; D000242:Cyclic AMP