title: Initiation of lambda DNA replication reconstituted with purified lambda and Escherichia coli replication proteins.
abstract: Using highly purified bacteriophage lambda and E. coli replication proteins, we were able to reconstitute an in vitro system capable of replication ori lambda-containing plasmid DNA. The addition of a new E. coli factor, the grpE gene product, to this replication system reduced the level of dnaK protein required for efficient DNA synthesis by at least 10-fold, and also allowed the isolation of a stable DNA replication intermediate. Based on all available information, we propose a molecular mechanism for the action of the dnaK and grpE proteins during the prepriming reaction leading to lambda DNA synthesis.
authors.affiliation: Department of Molecular Biology, University of Gdansk, Kladki, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Zylicz
authors.forename: D
authors.initials: D
authors.lastname: Ang
authors.forename: K
authors.initials: K
authors.lastname: Liberek
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: C
authors.initials: C
authors.lastname: Georgopoulos
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000235:genetics*; D004261:DNA Replication; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D014764:Viral Proteins; D004250:DNA Topoisomerases, Type II