title: Activities of enzymes of acetoacetate metabolism in rat brown adipose tissue during development.
abstract: The activities of two mitochondrial enzymes concerned in the utilization of acetoacetate, namely 3-oxoacid CoA-transferase and acetoacetyl-CoA thiolase, were high throughout the suckling and weanling period in brown adipose tissue of the rat. In contrast, 3-hydroxybutyrate dehydrogenase activity was comparatively low during this period. The activity of cytosolic acetoacetyl-CoA synthetase (involved in lipogenesis) declined after birth and remained low until the pups were weaned. Experiments with brown-adipose-tissue slices from weanling rats indicated that 70% of the [3-14C]acetoacetate utilized was oxidized to 14CO2, and this value was not altered appreciably by the addition of glucose and insulin.
authors.forename: D H
authors.initials: DH
authors.lastname: Williamson
authors.forename: V
authors.initials: V
authors.lastname: Ilic
mesh_terms: D000090:Acetoacetates / Q000378:metabolism*; D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000201:enzymology*; D000818:Animals; D003066:Coenzyme A Ligases / Q000378:metabolism; D015256:Coenzyme A-Transferases; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013466:Sulfurtransferases / Q000378:metabolism; D014886:Weaning
chemical_list: D000090:Acetoacetates; C016635:acetoacetic acid; D000101:Acetyl-CoA C-Acetyltransferase; D013466:Sulfurtransferases; D015256:Coenzyme A-Transferases; C021460:3-ketoacid CoA-transferase; D003066:Coenzyme A Ligases; C023747:acetoacetyl-CoA synthetase