Rapid induction of carcinomas and gamma-glutamyl transpeptidase-rich clones in N-methyl-N-benzylnitrosamine-treated hamster buccal pouch.
Topical application of N-methyl-N-benzylnitrosamine [(MBN) CAS: 937-40-6] to Syrian hamster buccal pouch epithelia resulted within 4 days in the induction of epithelial cell foci with histochemical gamma-glutamyl transpeptidase (GGTase) activity. Approximately 94% of the foci consisted of individual cells or nests of 8 cells or less, as visualized in wholemounts of GGTase-stained pouch epithelia. During a regimen of twice weekly MBN applications, the GGTase-stained foci increased in size to a maximum diameter of approximately 0.4 mm by the third week. In histologic sections, the enlarging foci were similar morphologically to those of the adjacent GGTase-negative epithelium. Groups of animals treated with MBN for 2, 4, and 8 weeks developed a buccal pouch tumor incidence of 43, 86, and 100%, respectively, within 39 weeks or less. Of the 138 papillomas and carcinomas examined, 11 contained patchy GGTase activity.
Solt
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Calderon-Solt
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Odajima
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D000818:Animals; D002273:Carcinogens; D002277:Carcinoma / Q000139:chemically induced*; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced; D002610:Cheek; D006224:Cricetinae; D004128:Dimethylnitrosamine / Q000031:analogs & derivatives* / Q000633:toxicity; D006416:Hematoxylin / Q000032:analysis; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000201:enzymology / Q000473:pathology; D009062:Mouth Neoplasms / Q000139:chemically induced*; D013274:Stomach Neoplasms / Q000139:chemically induced; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D002273:Carcinogens; C014707:nitrosobenzylmethylamine; D005723:gamma-Glutamyltransferase; D004128:Dimethylnitrosamine; D006416:Hematoxylin