Integration of Friend murine leukemia virus into both alleles of the p53 oncogene in an erythroleukemic cell line.
The Friend virus-transformed erythroleukemic cell line DP16-9B4 has undergone a complex rearrangement of the p53 oncogene and lacks any detectable expression of the p53 protein. We report here characterization of both p53 alleles in this cell line and identify independent integrations of Friend murine leukemia virus sequences into the coding region of both alleles.
Hicks
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Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada.
Mowat
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D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D005622:Friend murine leukemia virus / Q000235:genetics*; D005786:Gene Expression Regulation; D004915:Leukemia, Erythroblastic, Acute / Q000382:microbiology*; D009363:Neoplasm Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010750:Phosphoproteins / Q000235:genetics*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53
D004279:DNA, Viral; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53; D004262:DNA Restriction Enzymes