The construction and cloning of synthetic genes coding for artificial proteins and expression studies to obtain fusion proteins.
Synthetic genes coding for artificial proteins with predefined and nutritionally valuable amino acid compositions have been constructed and cloned in bacterial plasmid vector pKK233-2. The genes were constructed from three easily interchangeable 'cassettes' encoding either essential, non-essential or branched-chain amino acid residues. A potential hairpin loop structure in the mRNA around the region of the ribosome binding site was probably the reason for blockage of translation from this vector. Two selected genes, AHB (containing one copy of each cassette) and A6 (consisting of six copies concatemerized A cassette) were cloned into pUR300, a beta-Gal fusion vector and expressed as fusion proteins beta-Gal-AHB and beta-Gal-A6.
Biernat
J
J
BIOSYNTECH Biochemische Synthesetechnik GmbH, Hamburg, FRG.
Hasselmann
H
H
Hofer
B
B
Kennedy
N
N
Köster
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005782:Gels; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D005822:Genetic Vectors; D008025:Ligases / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*
D005782:Gels; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D004247:DNA; D004262:DNA Restriction Enzymes; D008025:Ligases