Regulation of an in vitro anti-DNA antibody response by thymocytes and T cells from NZB/W and normal mice.
The spontaneous in vitro anti-DNA antibody response generated by preautoimmune and many normal mouse spleen cells was suppressed by the addition of syngeneic thymocytes or splenic T cells. Suppressive activity was found in normal mice (DBA/2J) and to an equivalent degree in the autoimmune (New Zealand Black X New Zealand White)F1 (B/W) strain. The suppressor cells were cortisone-resistant, radiosensitive and carried Lyt 1 and Lyt 2 markers. Nonspecific suppression was not involved since the primary and primed in vitro anti-sheep erythrocyte (anti-SRBC) responses were unaffected. Both spontaneous and lipopolysaccharide-stimulated anti-DNA antibody responses could be suppressed. There was no difference in the suppressive activity of cells from young or old, normal or autoimmune mice. These T cells may therefore play a role in preventing the anti-DNA antibody response in normal and young B/W mice, but evidently fail to influence the development of in vivo anti-DNA autoimmune responses in the old B/W mice.
DeMoor
J M
JM
Bell
D A
DA
Singhal
S K
SK
D000375:Aging; D000818:Animals; D000917:Antibody Formation; D001323:Autoantibodies / Q000276:immunology*; D003348:Cortisone / Q000494:pharmacology; D004247:DNA / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000528:radiation effects
D001323:Autoantibodies; D008070:Lipopolysaccharides; D004247:DNA; D003348:Cortisone