Allosteric transition of erythrocyte alkaline phosphatase from Duchenne muscular dystrophy (DMD) patients and Duchenne muscular dystrophy carriers (Homo sapiens).
1. The kinetic properties of the p-nitrophenylphosphatase (EC 3.1.3.1) from erythrocytes was investigated in DMD-patients and DMD-carriers. 2. A different allosteric behaviour in the p-nitrophenylphosphatase from DMD-patients and DMD-carriers compared to controls is supported by the following findings: (a) values of n altered in F- inhibition of (K+)-activated p-nitrophenylphosphatase with Hill coefficients -1.5, -2.2 and -3.1; (b) heterotropic effect of increased concentration of Mg2+ on F- inhibition which is reverted by K+ in DMD-carriers and in control, but not in DMD-patients. 3. Evidence is presented showing that in DMD-patients and in DMD-carriers the interaction membrane-enzyme is different from the corresponding controls.
Goldemberg
A L
AL
Facultad de Ciencias Exactas y Naturales, U.N. de Mar del Plata, Argentina.
García
A M
AM
Fernández
H
H
Fortunato
M
M
Sánchez
J J
JJ
Trucco
R E
RE
D009597:4-Nitrophenylphosphatase / Q000037:antagonists & inhibitors / Q000097:blood*; D000469:Alkaline Phosphatase / Q000097:blood*; D000494:Allosteric Regulation; D004910:Erythrocyte Membrane / Q000201:enzymology* / Q000502:physiology; D005260:Female; D005459:Fluorides / Q000494:pharmacology; D006579:Heterozygote; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000502:physiology; D008297:Male; D009136:Muscular Dystrophies / Q000097:blood / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000097:blood*
D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase; D008274:Magnesium; D005459:Fluorides