title: Platelet factor XIII. The collagen receptor?
abstract: We have studied the binding of collagen fibers with platelet proteins using affinity chromatography on collagen-Sepharose. Only a few proteins from a platelet lysate were trapped by this column. When denatured collagen (gelatin) was used as the affinity ligand, the major protein did not bind and was identified as platelet Factor XIII by polyacrylamide gel electrophoresis, immunoprecipitation, and enzymic activity. This is a zymogen form of transglutaminase, which corresponds to the "a" subunit of the coagulation factor in plasma. Immunoglobulins specific for platelet Factor XIII obtained from antiserum raised against plasma Factor XIII were able to initiate platelet aggregation by themselves, in strong contrast to nonspecific antibodies. This specific immunoglobulin-mediated platelet aggregation required the presence of Ca2+. It was inhibited by aspirin and prostacyclin, but not by specific inhibitors for other agonists. These data suggest the possibility that the zymogen form of Factor XIII is located on the surface of platelets and may play a key role as the receptor for collagen-induced platelet aggregation.
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Imada
authors.forename: J
authors.initials: J
authors.lastname: Takagi
authors.forename: T
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Inada
mesh_terms: D000818:Animals; D000906:Antibodies; D002417:Cattle; D002448:Cell Adhesion; D003094:Collagen / Q000378:metabolism; D005176:Factor XIII / Q000378:metabolism*; D005779:Immunodiffusion; D008970:Molecular Weight; D010974:Platelet Aggregation; D038981:Receptors, Collagen; D011971:Receptors, Immunologic / Q000378:metabolism*; D011503:Transglutaminases / Q000378:metabolism
chemical_list: D000906:Antibodies; D038981:Receptors, Collagen; D011971:Receptors, Immunologic; D003094:Collagen; D005176:Factor XIII; D011503:Transglutaminases