title: Steady state levels of pro-dynorphin-related end products in the striatum and substantia nigra of the adult rhesus monkey.
abstract: Analysis of an acid extract of the striatum of the rhesus monkey revealed that the molar ratio of dynorphin A(1-8)-sized material and dynorphin (A(1-17)-sized material is approximately 1:1. In addition, the molar ratios of the dynorphin A-related end products to both dynorphin B(1-13)-sized material and alpha-neo-endorphin-sized material were approximately 1:1. Fractionation of an acid extract of the substantia nigra by gel filtration and reverse phase HPLC revealed the following molar ratios for pro-dynorphin-related end products. The molar ratio of dynorphin A(1-8) to dynorphin A(1-17) is approximately 6:1. The molar ratios of dynorphin A-related end products to dynorphin B(1-13) and alpha-neo-endorphin were approximately 0.5 and 0.8, respectively. Comparisons between proteolytic processing patterns of pro-dynorphin in the striatum and the substantia nigra of the rhesus monkey are considered. In addition, comparisons between pro-dynorphin processing in the substantia nigra of the rhesus monkey and the substantia nigra of the rat are discussed.
authors.forename: R M
authors.initials: RM
authors.lastname: Dores
authors.forename: H
authors.initials: H
authors.lastname: Akil
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000032:analysis*; D004399:Dynorphins / Q000031:analogs & derivatives / Q000032:analysis; D004723:Endorphins / Q000032:analysis; D004745:Enkephalins / Q000032:analysis*; D005260:Female; D008253:Macaca mulatta; D010446:Peptide Fragments / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis*; D011863:Radioimmunoassay; D013378:Substantia Nigra / Q000032:analysis*
chemical_list: D004723:Endorphins; D004745:Enkephalins; D010446:Peptide Fragments; D011498:Protein Precursors; C025681:alpha-neoendorphin; D004399:Dynorphins; C026770:dynorphin (1-8); C036200:rimorphin; C033660:preproenkephalin