[Classification of the isolated hepatocytes].
At this present, enzyme perfusion method is a routine technique to isolate hepatocytes from rat liver for the physiological and pathological experiments. This study described a way of the classification of freshly isolated hepatocytes. First of all, the hepatocytes were fractionated with parenchymal and non-parenchymal cells by low speed centrifugation. And then these cells were subfractionated with a newly developed Percoll linear density gradient method. The fractionated parenchymal cells were divided with cells of periportal and centrilobular areas, respectively. Furthermore, their characteristics were confirmed functionally and morphologically. Non-parenchymal cells (NPC) include Kupffer cells, endothelial cells and fat storing cells (FSC, Ito cells). These isolated NPC are fractionated with a method as mentioned above or centrifugal alutriation method. In this paper, fractionation and classification of Kupffer cells and FSC were discussed with the measurement of fluorescent intensity of vitamin A and the morphological observation of cytoskeleton in culture. Especially, transport of vitamin A into FSC were detected autoradiographically.
Nagamori
S
S
First Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
Fujise
K
K
Hasumura
S
S
Homma
S
S
Sujino
H
H
Matsuura
K
K
Kameda
H
H
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D051381:Rats; D014801:Vitamin A / Q000493:pharmacokinetics
D014801:Vitamin A; D003577:Cytochrome P-450 Enzyme System; D005952:Glucose-6-Phosphatase