The characteristics and distribution of monoamine oxidase (MAO) activity in different tissues of the rainbow trout, Salmo gairdneri.
Monoamine oxidase (MAO) activity was determined fluorometrically in brain, intestine, kidney and liver tissues of the rainbow trout, Salmo gairdneri. MAO activity was inhibited by various drugs in a concentration-related manner, with single sigmoid inhibition curves, the inhibitors of type A MAO, harmaline and clorgyline being more effective than deprenyl, an inhibitor of type B MAO. Intestine exhibited greatest MAO activity followed by liver and brain with kidney showing least activity. The Michaelis constants (Km) also showed variability between tissues. Inhibition of MAO by harmaline was non-competitive and dependent on the concentration of substrate present.
Edwards
D
D
Hall
T R
TR
Brown
J A
JA
D000818:Animals; D001921:Brain / Q000201:enzymology; D006246:Harmaline / Q000494:pharmacology; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D007735:Kynuramine / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D014018:Tissue Distribution; D014337:Trout
D007735:Kynuramine; D006246:Harmaline; D008995:Monoamine Oxidase