Effects of experimentally altered glutathione levels on life span, metabolic rate, superoxide dismutase, catalase and inorganic peroxides in the adult housefly, Musca domestica.
Effects of varied levels of glutathione, an intracellular redox buffer, were examined in the adult male housefly in order to study the inter-relationship between enzymic and non-enzymic antioxidant defenses. An increase of over 100% in the concentrations of glutathione was induced by the administration of 3 mM L-2-oxothiazolidine-4-carboxylate (LOC), which increases the intracellular level of cysteine. A decrease in glutathione concentration of up to 85% was achieved by the administration of L-buthionine-SR-sulfoximine (BUS), which irreversibly inhibits glutamylcysteine synthetase. Life spans of houseflies were shortened by a decrease in the glutathione concentration, but were not prolonged by augmentation of glutathione. Metabolic rate and superoxide dismutase activity were independent of glutathione concentration. H2O2 was increased by both experimental regimes, whereas catalase activity was decreased by BUS. Results suggest that catalase activity is influenced by glutathione concentration.
Allen
R G
RG
Toy
P L
PL
Newton
R K
RK
Farmer
K J
KJ
Sohal
R S
RS
D000818:Animals; D019328:Buthionine Sulfoximine; D002374:Catalase / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism*; D006793:Houseflies / Q000502:physiology*; D007700:Kinetics; D008017:Life Expectancy; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives* / Q000494:pharmacology; D010545:Peroxides / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid; D013482:Superoxide Dismutase / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology*; D053778:Thiazolidines
D010545:Peroxides; D013844:Thiazoles; D053778:Thiazolidines; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D011761:Pyrrolidonecarboxylic Acid; C029536:2-oxothiazolidine-4-carboxylic acid