Immunological properties of rat phosphoglycerate mutase isozymes.
In mammalian tissues three phosphoglycerate mutase (D-phosphoglycerate 2,3-phosphomutase, EC 5.4.2.1) isozymes result from the homo-dimeric and hetero-dimeric combinations of two subunits (types M and B). Whereas rabbit antisera against type M subunit (purified from rat muscle) and against type BB isozyme (purified from rat brain) possessed a high degree of specificity, both antisera reacted with type BB and MM isozymes, as demonstrated by immunoneutralization and ELISA. Both the M subunit and B subunit were more immunoreactive than their respective dimeric isozymes. Subunits type M and B may possess common antigenic determinants, and some of these determinants may be sterically hindered in their dimeric structures.
Castellá
J
J
Unitat de Bioquímica, Facultat de Medicina, Universitat de Barcelona, Spain.
Ureña
J
J
Ludevid
D
D
Carreras
J
J
Climent
F
F
D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007527:Isoenzymes / Q000276:immunology*; D008970:Molecular Weight; D010770:Phosphotransferases / Q000276:immunology*; D051381:Rats
D007527:Isoenzymes; D010770:Phosphotransferases; D001731:Bisphosphoglycerate Mutase