Isolation of a yeast protein kinase gene by screening with a mammalian protein kinase cDNA.
The gene for a new Saccharomyces cerevisiae protein kinase was identified and isolated by hybridization to a cloned cDNA for the catalytic subunit of bovine cAMP-dependent protein kinase. This newly isolated yeast gene encodes a 680-amino-acid protein that shows strong sequence similarity to several serine/threonine protein kinases. The protein kinase catalytic domain is located at the carboxy-terminal portion of the protein. A large, amino-terminal domain shows no sequence similarity to any known protein kinase. It seems likely that the amino-terminal domain mediates the effects of an unknown regulator of the newly identified yeast protein kinase.
Maurer
R A
RA
Department of Physiology and Biophysics, University of Iowa, Iowa City 52242.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D005796:Genes; D005800:Genes, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D004271:DNA, Fungal; D004247:DNA; D011494:Protein Kinases; D004262:DNA Restriction Enzymes