title: Kinetic studies of the gastric H,K-ATPase. Evidence for simultaneous binding of ATP and inorganic phosphate.
abstract: The steady state rate of ATP hydrolysis (v) by the gastric H,K-ATPase and the steady state level of phosphoenzyme (E-P) have been measured at 0 and 10 mM KCl; both v and E-P have a nonhyperbolic dependence on the ATP concentration that is consistent with negative cooperativity. The ratio of the rate of hydrolysis to phosphoenzyme (v/[E-P]) was found to vary with the concentration of ATP. Thus, for the rate law v = [E-P].k, k must be a function of the ATP concentration. This requires that ATP be able to bind to E-P or to an enzyme form that occurs after E-P but prior to an irreversible step, such as the loss of inorganic phosphate (Pi). At low ATP concentrations, product inhibition by Pi gives concave downward plots of 1/v against Pi concentration. Pi increases the apparent Km and decreases the apparent Vm. At saturating ATP concentrations, Pi is a noncompetitive inhibitor. These data show that ATP and Pi can bind to the H,K-ATPase simultaneously. They are inconsistent with mechanisms where the binding of ATP and Pi is mutually exclusive.
authors.affiliation: Department of Physiology-Anatomy, University of California, Berkeley 94720.
authors.forename: W W
authors.initials: WW
authors.lastname: Reenstra
authors.forename: J D
authors.initials: JD
authors.lastname: Bettencourt
authors.forename: J G
authors.initials: JG
authors.lastname: Forte
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D017506:H(+)-K(+)-Exchanging ATPase; D007700:Kinetics; D008433:Mathematics; D008861:Microsomes / Q000201:enzymology*; D008962:Models, Theoretical; D010710:Phosphates / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D011485:Protein Binding; D013270:Stomach / Q000201:enzymology*; D013552:Swine
chemical_list: D010710:Phosphates; D010750:Phosphoproteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase