Glycosylphosphatidylinositol is involved in the membrane attachment of proteins in granules of chromaffin cells.
Incubation at 37 degrees C or treatment of granule membranes of chromaffin cells with Staphylococcus aureus phosphatidylinositol-specific phospholipase C converted from an amphiphilic to a hydrophilic form two proteins with molecular masses of 82 and 68 kDa respectively. Their release is time- and enzyme-concentration-dependent. We showed that they were immunoreactive with an anti-(cross-reacting determinant) antibody known to be revealed only after removal of a diacylglycerol anchor. Furthermore, the action of HNO2 suggests the presence of a non-acetylated glucosamine residue in the determinant. This is one of the first reports suggesting that a glycosylphosphatidylinositol anchor might exist in membranes other than the plasma membrane. We showed that the 68 kDa protein is probably not the subunit of dopamine (3,4-dihydroxyphenethylamine) beta-hydroxylase, an enzyme present in granules in both soluble and membrane-associated forms.
Fouchier
F
F
Institut de Chimie Biologigue, CNRS UA202, Université d'Aix-Marseille I, France.
Bastiani
P
P
Baltz
T
T
Aunis
D
D
Rougon
G
G
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002837:Chromaffin Granules / Q000378:metabolism*; D002838:Chromaffin System / Q000378:metabolism*; D003429:Cross Reactions; D003902:Detergents; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols; D007122:Immunoelectrophoresis; D008565:Membrane Proteins / Q000302:isolation & purification; D009608:Nitrous Acid; D017830:Octoxynol; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011092:Polyethylene Glycols; D011506:Proteins / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology
D003902:Detergents; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D011506:Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D004299:Dopamine beta-Hydroxylase; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D009608:Nitrous Acid