Uptake and secretion of technetium pertechnetate by the rat parotid gland.
The ability of acinar cells of the rat parotid gland to transport technetium pertechnetate (99mTcO-4) was examined. After intravenous injection, 99mTcO-4 was rapidly detected in parotid saliva. There was an excellent correlation between saliva and plasma 99mTcO-4 levels. The saliva to plasma ratio was always less than 1, consistent with the inability of rat parotid gland duct cells to concentrate the anion. Output of 99mTcO-4 by the parotid gland closely mimicked fluctuations in parotid saliva flow rate. In vitro, enzymatically dispersed parotid acinar cells accumulated 99mTcO-4 from the incubation medium in a biphasic manner. This uptake was partially blocked by 10(-4) M NaI. Cells which had accumulated 99mTcO-4 showed increased radionuclide efflux after exposure to 10(-5) M carbachol.
Fox
P C
PC
Bodner
L
L
Bowers
M R
MR
Baum
B J
BJ
D000818:Animals; D001692:Biological Transport; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D010306:Parotid Gland / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000032:analysis; D013670:Sodium Pertechnetate Tc 99m / Q000378:metabolism*
D013670:Sodium Pertechnetate Tc 99m