Microdetermination of serum D-amino acids.
A method for the quantitative determination of serum D-amino acids in the range 0.5-20 nmol is described. In the method alpha-keto acids, derived from D-amino acids by D-amino acid oxidase, are measured as hydrazones. The method is unresponsive to the presence of a large excess of L-amino acids. It allows a fast assay in a small amount of specimen (0.1 ml), with good reproducibility and accuracy.
Nagata
Y
Y
Akino
T
T
Ohno
K
K
D000409:Alanine / Q000097:blood; D000596:Amino Acids / Q000097:blood*; D003605:D-Amino-Acid Oxidase; D005182:Flavin-Adenine Dinucleotide; D006801:Humans; D008832:Microchemistry; D010649:Phenylalanine / Q000097:blood; D010949:Plasma / Q000032:analysis; D013053:Spectrophotometry
D000596:Amino Acids; D005182:Flavin-Adenine Dinucleotide; D010649:Phenylalanine; D003605:D-Amino-Acid Oxidase; D000409:Alanine