title: Rapid induction of carcinomas and gamma-glutamyl transpeptidase-rich clones in N-methyl-N-benzylnitrosamine-treated hamster buccal pouch.
abstract: Topical application of N-methyl-N-benzylnitrosamine [(MBN) CAS: 937-40-6] to Syrian hamster buccal pouch epithelia resulted within 4 days in the induction of epithelial cell foci with histochemical gamma-glutamyl transpeptidase (GGTase) activity. Approximately 94% of the foci consisted of individual cells or nests of 8 cells or less, as visualized in wholemounts of GGTase-stained pouch epithelia. During a regimen of twice weekly MBN applications, the GGTase-stained foci increased in size to a maximum diameter of approximately 0.4 mm by the third week. In histologic sections, the enlarging foci were similar morphologically to those of the adjacent GGTase-negative epithelium. Groups of animals treated with MBN for 2, 4, and 8 weeks developed a buccal pouch tumor incidence of 43, 86, and 100%, respectively, within 39 weeks or less. Of the 138 papillomas and carcinomas examined, 11 contained patchy GGTase activity.
authors.forename: D B
authors.initials: DB
authors.lastname: Solt
authors.forename: L
authors.initials: L
authors.lastname: Calderon-Solt
authors.forename: T
authors.initials: T
authors.lastname: Odajima
mesh_terms: D000818:Animals; D002273:Carcinogens; D002277:Carcinoma / Q000139:chemically induced*; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced; D002610:Cheek; D006224:Cricetinae; D004128:Dimethylnitrosamine / Q000031:analogs & derivatives* / Q000633:toxicity; D006416:Hematoxylin / Q000032:analysis; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000201:enzymology / Q000473:pathology; D009062:Mouth Neoplasms / Q000139:chemically induced*; D013274:Stomach Neoplasms / Q000139:chemically induced; D005723:gamma-Glutamyltransferase / Q000032:analysis*
chemical_list: D002273:Carcinogens; C014707:nitrosobenzylmethylamine; D005723:gamma-Glutamyltransferase; D004128:Dimethylnitrosamine; D006416:Hematoxylin