Opiate receptor-mediated chemotaxis of human monocytes.
The opiate peptides, D-ala-D-leuenkephalin, beta-endorphin and dynorphin, [1-13] are potent stimulators of human mononuclear cell chemotaxis. Peak responses were observed in the range of 10(-8) - 10(-11)M with activity detectable at 10(-14)M. Morphine was without effect in this assay. Incubation of cells with (-) naloxone but not (+) naloxone completely blocked activity, supporting stereospecific opiate receptor-mediated chemotaxis. Opiates and other neuropeptides are discussed as components of a network whose purpose is to integrate brain and behavioral modalities with immune function.
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D002633:Chemotaxis / Q000187:drug effects; D004399:Dynorphins / Q000494:pharmacology; D004723:Endorphins / Q000494:pharmacology; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D016308:Enkephalin, Leucine-2-Alanine; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000502:physiology*; D010446:Peptide Fragments / Q000494:pharmacology; D011957:Receptors, Opioid / Q000502:physiology*; D001615:beta-Endorphin
D004723:Endorphins; D010446:Peptide Fragments; D011957:Receptors, Opioid; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D001615:beta-Endorphin; C022118:enkephalinamide-Met, Ala(2)-; D016308:Enkephalin, Leucine-2-Alanine; C026843:dynorphin (1-13); D004399:Dynorphins