Regulation of acetyl-CoA carboxylase by glucagon in HeLa cells.
HeLa cells cultured in medium containing lipid-free fetal bovine serum or in Waymouth's serum-free medium showed an increase in acetyl-CoA carboxylase (ACC) activity of 8- and 4-fold respectively as compared to cells cultured in medium containing fetal bovine serum. This increase in ACC activity was associated with a corresponding increase in the relative synthesis of ACC. Addition of glucagon (1.5 microgram/ml) to the HeLa cell culture medium caused a 50% decrease in ACC activity. This was accompanied by a concordant decrease in the relative synthesis of ACC as measured by immunochemical techniques.
Bhullar
R P
RP
Dakshinamurti
K
K
D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis / Q000378:metabolism*; D003470:Culture Media; D005227:Fatty Acids / Q000096:biosynthesis; D005934:Glucagon / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008025:Ligases / Q000378:metabolism*
D003470:Culture Media; D005227:Fatty Acids; D005934:Glucagon; D008025:Ligases; D000103:Acetyl-CoA Carboxylase