Effects of epidermal growth factor and nerve growth factor on isoproterenol-induced DNA synthesis in rat parotid and pancreas following removal of submandibular-sublingual glands.
When epidermal (EFG) (10 ng/kg body wt) or nerve growth factor (NGF) (1 ng/kg body wt) was given intraperitoneally to sialadenectomized young rats (submandibular-sublingual (SM-SL) glands removed) prior to injection of isoproterenol (ISO) (50 mg/kg body wt), the inhibition of ISO-induced thymidine incorporation into DNA of parotid gland and pancreas caused by removal SM-SL glands was reversed, and thymidine incorporation of sialadenectomized ISO-treated organs was as high as that of parotid and pancreas of surgically intact animals given ISO. EGF alone caused an increase in [3H] thymidine incorporation into DNA of parotid (63%) and pancreas (59%); removal of the SM-SL glands caused a decrease of 57-70% in thymidine incorporation into DNA of parotid, pancreas, liver, lung, kidney, and spleen. A growth effect attributable to the EGF and NGF of the submandibular gland was thus apparent for all organs examined, but even if they had large complements of beta 1 adrenoceptors, only the exocrine organs showed the ISO-induced beta 1 adrenoceptor response to EGF and NGF. EGF and NGF thus interact only with beta 1 adrenoceptors of exocrine organs to cause marked increase in [3H] thymidine incorporation of these organs.
Schneyer
C A
CA
Department of Physiology and Biophysics, University of Alabama, Birmingham.
Humphreys-Beher
M
M
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009929:Organ Size; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010306:Parotid Gland / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D013361:Sublingual Gland / Q000502:physiology* / Q000601:surgery; D013363:Submandibular Gland / Q000502:physiology* / Q000601:surgery; D014316:Tritium / Q000378:metabolism
D009414:Nerve Growth Factors; D011943:Receptors, Adrenergic, beta; D014316:Tritium; D004815:Epidermal Growth Factor; D004247:DNA; D007545:Isoproterenol