title: Detection of B-cell lymphoma in peripheral blood by DNA hybridisation.
abstract: As an alternative to morphological identification of circulating neoplastic lymphocytes in the blood of patients with non-Hodgkin's lymphoma, DNA of peripheral blood lymphocytes was analysed for clonal immunoglobulin gene rearrangements in 29 patients with low-grade B-cell lymphomas. 76% of the patients showed clonal rearrangements of immunoglobulin genes in their blood, including 58% of those with no other evidence of disease. In seven patients from whom paired samples were available the rearranged bands found in the blood and in lymph-node biopsy specimens containing histologically confirmed lymphoma were identical. Detection of circulating lymphoma cells by use of tumour-specific anti-idiotype antibodies and cytofluorimetry showed complete agreement with the results of DNA analysis.
authors.forename: E
authors.initials: E
authors.lastname: Hu
authors.forename: M
authors.initials: M
authors.lastname: Trela
authors.forename: J
authors.initials: J
authors.lastname: Thompson
authors.forename: J
authors.initials: J
authors.lastname: Lowder
authors.forename: S
authors.initials: S
authors.lastname: Horning
authors.forename: R
authors.initials: R
authors.lastname: Levy
authors.forename: J
authors.initials: J
authors.lastname: Sklar
mesh_terms: D001402:B-Lymphocytes; D003001:Cloning, Molecular; D004273:DNA, Neoplasm; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008223:Lymphoma / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D009693:Nucleic Acid Hybridization
chemical_list: D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains