The in vitro metabolism of Eulan WA New by liver homogenates from freshwater fish.
The rate of formation of pentachloro-2-aminodiphenylether (5-PAD) from pentachloro-2-(chloromethylsulphonamido) diphenylether (6-PCSD) by goldfish liver homogenates was studied in a variety of buffers. The highest rate was observed when the homogenate was prepared and assayed in phosphate buffer containing dithiothreitol. The 5-PAD forming activity of liver homogenate preparations from a variety of freshwater fish (goldfish, carp, pike, rainbow trout, perch and eel) showed contrasting species differences. These results could be correlated with information available on the toxicity of the mothproofing agent Eulan WA New (which contains PCSDs) to these fish.
Machon
A
A
North
M J
MJ
Price
N C
NC
Wells
D E
DE
D000818:Animals; D002021:Buffers; D002733:Chlorophenols / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004229:Dithiothreitol / Q000494:pharmacology; D006054:Goldfish; D007306:Insecticides / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*
D002021:Buffers; D002733:Chlorophenols; D007306:Insecticides; C034414:Eulan WA new; D004229:Dithiothreitol