Association of [14C]glutamate with the protein components of synaptic membrane preparations.
Calf brain synaptic plasma membranes were saturated under extracellular conditions with [14C]glutamic acid and the resulting labelled membranes fractionated with 0.9% NaCl, distilled water, n-butanol-water, 0.05 mol/L NaOH and 0.5% Triton X-100 solutions in this order. Triton X-100 was the most effective solubilizer, liberating altogether about 24% of the membrane proteins, but only 4-7% of the label, while NaOH was the most potent solubilizer for the protein-bound label (50-70%). Slab gel electrophoresis showed significant qualitative differences in the banding patterns of the protein extracts, but only two fractions, a low-molecular weight (around 15 kd) and a high-molecular weight (greater than 200 kd) fraction bound [14C]glutamate.
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D000818:Animals; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate; D013570:Synaptic Membranes / Q000378:metabolism*
D005971:Glutamates; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012967:Sodium Dodecyl Sulfate; D018698:Glutamic Acid