title: Evidence in liver for a disulphide-linked scavenger receptor containing a binding site for acetylated low-density lipoprotein and maleylated bovine serum albumin.
abstract: Membranes from rat liver were analysed under reducing conditions. The components of the soluble membranes responsible for the binding of acetylated low density lipoprotein (acetyl-LDL) and maleylated bovine serum albumin (Mal-BSA) were chromatographed on a polyethyleneimine-cellulose column and subsequently separated by gel electrophoresis. For both ligands a major binding protein (Mr = 35,000) was revealed by ligand blotting. A minor protein (Mr greater than 67,000) exhibited little binding. The Scatchard plot of the 131I-Mal-BSA binding data of the 35 kDa protein was linear, with a Kd of 17.3 nM. High concentrations of acetyl-LDL competed for half of the 131I-Mal-BSA binding. Excessive Mal-BSA competed for all the visible acetyl-LDL binding. The findings indicate the existence, in the reduced hepatic membrane, of a 35 kDa protein that has two binding sites for 131I-Mal-BSA and one binding site for acetyl-LDL.
authors.affiliation: Department of Medicine, University of Heidelberg, Federal Republic of Germany.
authors.forename: E
authors.initials: E
authors.lastname: Ottnad
authors.forename: D P
authors.initials: DP
authors.lastname: Via
authors.forename: H
authors.initials: H
authors.lastname: Sinn
authors.forename: E
authors.initials: E
authors.lastname: Friedrich
authors.forename: R
authors.initials: R
authors.lastname: Ziegler
authors.forename: H A
authors.initials: HA
authors.lastname: Dresel
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D015815:Cell Adhesion Molecules; D004220:Disulfides; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008024:Ligands; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D051381:Rats; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011973:Receptors, LDL / Q000378:metabolism*; D051116:Receptors, Scavenger; D012710:Serum Albumin, Bovine / Q000378:metabolism*
chemical_list: D000418:Albumins; D015815:Cell Adhesion Molecules; D004220:Disulfides; D008024:Ligands; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D011973:Receptors, LDL; D051116:Receptors, Scavenger; C032629:acetyl-LDL; C049877:maleylalbumin; C049878:maleylalbumin receptor; D012710:Serum Albumin, Bovine