Specific interaction between tRNA and its cognate amino acid as detected by circular dichroism and fluorescence spectroscopy.
Specific interaction was detected between tRNA and its cognate L-amino acid by circular dichroism (CD) and fluorescence spectroscopy; fluorescence spectral change with saturation was observed when tRNAAsp was titrated only with L-aspartic acid, but not with D-aspartic acid, L- and D-glutamic acids. It was also the case for tRNA2Glu and L-glutamic acid as detected by CD. These results are consistent with the hypothesis that the anticodon, or the complex of four nucleotides (C4N) of the anticodon three bases and the discriminator base in a tRNA (Shimizu, M., J. Mol. Evol. (1982) 18, 297-303) participates in recognition of amino acids.
Watanabe
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Miura
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D000926:Anticodon; D001224:Aspartic Acid / Q000494:pharmacology*; D002942:Circular Dichroism; D004926:Escherichia coli / Q000235:genetics; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D007536:Isomerism; D007700:Kinetics; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl; D013050:Spectrometry, Fluorescence
D000926:Anticodon; D005971:Glutamates; D012346:RNA, Transfer, Amino Acyl; D001224:Aspartic Acid; D018698:Glutamic Acid