title: 31P NMR measurement of ATP synthesis rate in perfused intact rat hearts.
abstract: Using 31P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 +/- 2.5 (SE) X 10(3) mmHg X min-1 and consuming O2 at a rate of 35 +/- 2 mumol O2 X min-1 X (g dry wt)-1, at 37 degrees C. This rate was 7.2 +/- 0.9 mumol X s-1 X (g dry wt)-1 and was related to the rate of oxygen atom consumption by a ratio of 6.3 +/- 0.9. These data show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.
authors.forename: P
authors.initials: P
authors.lastname: Kingsley-Hickman
authors.forename: E Y
authors.initials: EY
authors.lastname: Sako
authors.forename: P A
authors.initials: PA
authors.lastname: Andreone
authors.forename: J A
authors.initials: JA
authors.lastname: St Cyr
authors.forename: S
authors.initials: S
authors.lastname: Michurski
authors.forename: J E
authors.initials: JE
authors.lastname: Foker
authors.forename: A H
authors.initials: AH
authors.lastname: From
authors.forename: M
authors.initials: M
authors.lastname: Petein
authors.forename: K
authors.initials: K
authors.lastname: Ugurbil
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010101:Oxygen Consumption; D010477:Perfusion; D010710:Phosphates / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000032:analysis; D051381:Rats
chemical_list: D010710:Phosphates; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases