[Immunoradiometric analysis of rat myeloperoxidase].
The immunoradiometric assay of rat myeloperoxidase (MPO) was developed based on the usage of the CNBr-activated sepharose as a solid phase for antiMPO and of [125I] IgG (the affinity preparation of antiMPO) as a label. The analysis lasted for 8 h and standard curve limits were 0.5-500 ng per tube. Intra and inter assay coefficients of variation did not exceed 3% and 6.5%, respectively. It was shown by the above method that the intensive swimming of male rats led to an increase in the MPO concentration in blood plasma and to its decrease in neutrophils.
Tsyplenkov
P V
PV
Morozov
V I
VI
Kokriakov
V N
VN
Rogozkin
V A
VA
D000818:Animals; D008297:Male; D009504:Neutrophils / Q000201:enzymology; D009195:Peroxidase / Q000097:blood*; D005082:Physical Exertion; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats
D009195:Peroxidase
Immunoradiometricheskiĭ analiz mieloperoksidazy krysy.