Assignment of ecto-5'-nucleotidase to human chromosome 6.
Ecto-5'-nucleotidase activity (5NT) was measured on whole cells of 26 human x Chinese hamster hybrids. Concordance analysis showed 100% correlation between enzyme activity and inheritance of human chromosome 6. This observation was confirmed by a segregation analysis in which cells of a hybrid containing chromosome 6 were stained by indirect immunofluorescence for HLA Class 1 antigen and sorted by a fluorescence-activated cell sorter (FACS). Cells in the HLA- compartment were cloned and expression of HLA and 5NT was determined. Of nine clones, three were HLA-, 5NT- and six were HLA+, 5NT+, supporting the linkage of 5NT to chromosome 6.
Boyle
J M
JM
Department of Biochemical Genetics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
Hey
Y
Y
Guerts van Kessel
A
A
Fox
M
M
D015720:5'-Nucleotidase; D000818:Animals; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D005805:Genes, MHC Class I; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D009708:Nucleotidases / Q000235:genetics*; D010641:Phenotype
D005819:Genetic Markers; D009708:Nucleotidases; D015720:5'-Nucleotidase