Presence of phosphatidylcholine hydroperoxide in human plasma.
A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cytochrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2,000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.
Miyazawa
T
T
Department of Food Chemistry, Faculty of Agriculture, Tohoku University, Miyagi.
Yasuda
K
K
Fujimoto
K
K
Kaneda
T
T
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003574:Cytochrome c Group; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000097:blood*
D003574:Cytochrome c Group; D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide; D008165:Luminol