title: The effect of sodium intake on the renin response to dopamine in superfused rat renal cortical cells.
abstract: In addition to its natriuretic effect in the kidney, dopamine can also stimulate renin release. This study employs an in vitro system to examine the relationship between sodium intake and dopamine-induced renin release. Enzymically dispersed renal cortical cells obtained from rats on low, normal and high sodium diets were superfused at 37 degrees C with Krebs buffer [0.2% glucose, 0.2% bovine serum albumin (BSA)]. Dopamine was given as 10-min infusions and renin activity was measured in the superfusate as angiotensin I generated following incubation of samples with excess nephrectomized sheep renin substrate. Rats on a low sodium diet showed a higher basal cellular renin release and an enhanced renin response to dopamine (10(-7), 10(-6) mol/l) compared with the normal sodium diet group. Rats on a high sodium diet, in contrast, showed a lower cellular renin release and a decreased sensitivity to dopamine (10(-7), 10(-6) mol/l) compared with the normal sodium diet group. These results demonstrate that the renin response to dopamine is dependent upon sodium intake.
authors.affiliation: Department of Medicine, Western General Hospital, Edinburgh, Scotland, UK.
authors.forename: B C
authors.initials: BC
authors.lastname: Williams
authors.forename: A
authors.initials: A
authors.lastname: Eglen
authors.forename: F M
authors.initials: FM
authors.lastname: Duncan
authors.forename: C R
authors.initials: CR
authors.lastname: Edwards
mesh_terms: D000803:Angiotensin I / Q000378:metabolism; D000818:Animals; D004298:Dopamine / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012083:Renin / Q000096:biosynthesis*; D012982:Sodium, Dietary / Q000494:pharmacology*
chemical_list: D012982:Sodium, Dietary; D000803:Angiotensin I; D012083:Renin; D004298:Dopamine