Role of stearoyl-CoA desaturase and 1-acylglycerophosphorylcholine acyltransferase in the regulation of the acyl composition of phosphatidylcholine in rat liver.
The role of stearoyl-CoA desaturase and 1-acylglycerophosphorylcholine (1-acylGPC) acyltransferase in regulating acyl composition of microsomal phosphatidylcholine was investigated in rat liver, using rats in five different kinds of physiological state: clofibric acid-fed rats, diabetic rats, insulin-treated diabetic rats, starved rats and starved-refed rats. There was a reverse linear correlation between 18:1 and 18:2 in the C-2 position, and a similar correlation was found between 18:1 and 18:2 in microsomal free fatty acids. The proportion of 18:1 or 18:2 in the C-2 position of phosphatidylcholine correlated with the proportion of the respective fatty acids in microsomal free unsaturated fatty acids which could be incorporated effectively, except for the group of clofibric acid-fed rats. In this group alone, 1-acylGPC acyltransferase was induced markedly. The proportion of 18:1 in microsomal free fatty acids correlated well with the activity of stearoyl-CoA desaturase. The physiological significance of stearoyl-CoA desaturase and 1-acylGPC acyltransferase was discussed in relation to the regulation of the acyl composition of phosphatidylcholine.
Kawashima
Y
Y
Hirose
A
A
Adachi
T
T
Kozuka
H
H
D008243:1-Acylglycerophosphocholine O-Acyltransferase; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002995:Clofibric Acid / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005502:Food; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000201:enzymology; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*
D002995:Clofibric Acid; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase; C031076:2-acylglycerophosphocholine acyltransferase