Identification of parathyroid hormone messenger ribonucleic acid in an apparently nonfunctioning parathyroid carcinoma transformed from a parathyroid carcinoma with hyperparathyroidism.
mRNA coding for pre-pro-PTH, a precursor of PTH, was sought in an apparently nonfunctioning parathyroid carcinoma that had transformed from one that was previously functioning. Total poly(A+) RNA was prepared by phenol-chloroform-isoamyl alcohol extraction and oligo-dT-cellulose affinity chromatography from the tumor tissue and bovine parathyroid glands. In the rabbit reticulocyte lysate cell-free translation system, total poly(A+) RNA from the tumor as well as that from bovine parathyroid glands directed the translation of a product which was specifically precipitated by an anti-PTH serum and which migrated at the same position as pre-pro-PTH on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results indicated the presence of mRNA coding for pre-pro-PTH (PTH mRNA) in an apparently nonfunctioning parathyroid carcinoma, suggesting that PTH synthesis is not always absent in parathyroid carcinomas which are not accompanied by hyperparathyroidism.
Baba
H
H
Kishihara
M
M
Tohmon
M
M
Fukase
M
M
Kizaki
T
T
Okada
S
S
Matsuzuka
F
F
Kobayashi
A
A
Kuma
K
K
Fujita
T
T
D000818:Animals; D002474:Cell-Free System; D005260:Female; D006801:Humans; D006961:Hyperparathyroidism / Q000209:etiology; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D010281:Parathyroid Hormone / Q000096:biosynthesis / Q000235:genetics*; D010282:Parathyroid Neoplasms / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D011061:Poly A / Q000032:analysis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification*; D012334:RNA, Neoplasm / Q000302:isolation & purification; D011817:Rabbits
D010281:Parathyroid Hormone; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011061:Poly A