title: Relationship of human cord blood mononuclear cell lines, established by the HTLV-I integration, to expression of T-cell phenotypes and the T-cell receptor gene rearrangement.
abstract: The HTLV-I integrated cell lines originated from human cord blood mononuclear cells were examined for the T-cell receptor beta chain gene rearrangement in conjunction with immunological phenotype analysis. Five of seven cell lines contained the rearranged T-cell receptor beta chain gene. Interestingly, most of the rearranged lines failed to express the T cell differentiation antigens, OKT 4 and 8, though their positive expression of Leu 1 antigen indicated the commitment to the T-cell lineage. On the other hand, two cell lines retained the germ-line configuration of the beta chain gene, while phenotypical examination revealed that the two cell lines have distinct T-cell antigens. Non-rearranged cell lines have proved to be nearly monoclonal from HTLV-I integration pattern and/or surface marker clonality. Thus, the relationship between the immunological phenotypes and the T-cell receptor gene rearrangement observed in the T-cell lineage is not strict and often dissociates. A possible involvement of HTLV-I infection on the dissociation between phenotype and genotype is discussed from the viewpoint of phenotypical modulation.
authors.affiliation: Institute for Virus Research, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Miwa
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Kita
authors.forename: S
authors.initials: S
authors.lastname: Shirakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Koyanagi
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
authors.forename: A
authors.initials: A
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Honjo
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
mesh_terms: D000954:Antigens, Surface / Q000235:genetics; D002461:Cell Line, Transformed; D005312:Fetal Blood / Q000276:immunology; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000954:Antigens, Surface