Cooperation in oncogenesis between BK virus early region gene and the activated human c-Harvey ras oncogene.
Rapidly growing, undifferentiated brain tumours were induced in newborn Syrian hamsters by intracerebral inoculation of a recombinant DNA (pBK/c-rasA) carrying the BK virus (BKV) early region gene and the activated human c-Harvey-ras (c-Ha-ras) oncogene. Neither of the two genes inoculated alone nor recombinant DNA of the BKV early region gene and the normal human c-Ha-ras proto-oncogene were tumourigenic. Tumour-derived cell lines propagated in culture were immortalized and had growth characteristics consistent with a fully transformed phenotype. Tumours and tumour cell lines contained pBK/c-rasA sequences integrated into cellular DNA and expressed BKV- and c-Ha-ras-specific transcripts as well as BKV T antigen and c-Ha-ras p21. These findings are discussed in relation to a possible cooperation or synergism between BKV and cellular oncogenes in human neoplasia.
Corallini
A
A
Institute of Microbiology, School of Medicine, University of Ferrara, Italy.
Pagnani
M
M
Caputo
A
A
Negrini
M
M
Altavilla
G
G
Catozzi
L
L
Barbanti-Brodano
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D001739:BK Virus / Q000235:genetics*; D001932:Brain Neoplasms / Q000209:etiology*; D002472:Cell Transformation, Viral; D003043:Cocarcinogenesis; D006224:Cricetinae; D004247:DNA; D015342:DNA Probes; D004274:DNA, Recombinant / Q000008:administration & dosage; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D005822:Genetic Vectors; D015151:Immunoblotting; D008647:Mesocricetus; D009857:Oncogenes; D011120:Polyomavirus / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D015183:Restriction Mapping
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D004274:DNA, Recombinant; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004247:DNA