Detection of Leishmania within sand flies by kinetoplast DNA hybridization.
A kinetoplast DNA hybridization probe method was used to detect Leishmania within sand flies and to distinguish it from the non-pathogenic flagellate, Endotrypanum. Eighty-one sand flies (74 Lutzomyia umbratilis, 1 Lu. anduzei, and 6 Lu. shannoni) collected outside Manaus, Brazil were dissected. Forty-four of these were found to be infected with flagellates, and 2 hybridized with a Leishmania braziliensis probe. Thirty-three of sixty-one flies reprobed with an Endotrypanum probe were positive.
Rogers
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Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115.
Burnheim
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Wirth
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D000818:Animals; D015342:DNA Probes; D004270:DNA, Circular / Q000032:analysis*; D018105:DNA, Kinetoplast; D056890:Eukaryota / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D007303:Insect Vectors / Q000469:parasitology*; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D011576:Psychodidae / Q000469:parasitology*
D015342:DNA Probes; D004270:DNA, Circular; D018105:DNA, Kinetoplast