title: Glycoprotein-specific immune responses in cats after exposure to feline herpesvirus-1.
abstract: To obtain synchronous infection, 10 cats were inoculated with feline herpesvirus-1 (FHV-1) on the oral, nasal, and conjunctival mucosa. Swab specimens of the nasal, conjunctival, and pharyngeal mucosa were obtained for virus isolation from each cat before inoculation and at 3-day intervals thereafter until postinoculation day 21. Recovery of virus and evidence of clinical signs were used to document FHV-1 infection. Serum was obtained from blood samples collected sequentially from each cat between day 0 and postinoculation day 90. Virus-neutralizing antibody titer was determined in all serum specimens. Immunoprecipitation with [35S]methionine- and [14C]glucosamine-labeled viral antigens, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was performed on each specimen. Three precipitation bands with approximate molecular weights of 105,000, 68,000, and 60,000 were separated from [14C]glucosamine- and [35S]methionine-labeled immunoprecipitates. The concurrent detection of virus-neutralizing antibody and glycoprotein-specific immunoprecipitins implied that in cats, the FHV-1 glycoproteins were important in the induction of virus-neutralizing antibodies to FHV-1.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824.
authors.forename: D C
authors.initials: DC
authors.lastname: Burgener
authors.forename: R K
authors.initials: RK
authors.lastname: Maes
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D002460:Cell Line; D006023:Glycoproteins / Q000276:immunology*; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary*; D009500:Neutralization Tests; D011233:Precipitin Tests
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins