Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum.
A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation.
Roberts
I
I
Holmes
W M
WM
Hylemon
P B
PB
D003013:Clostridium / Q000235:genetics*; D003016:Clostridium perfringens / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006863:Hydrogen-Ion Concentration; D010957:Plasmids
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes