title: Difference in cell surface 5'-nucleotidase activity, protein iodination and membrane properties between mouse lymphoma and rat adenocarcinoma cell variants with low and high-metastatic potential.
abstract: 1. Distinct differences were found for the specific, concanavalin A-inhibitable 5'-nucleotidase activity, exhibiting 2-7 times higher values in the low-metastasizing cell variants. 2. Iodination of the cell surface proteins revealed a more heterogeneous labelling pattern of the high-malignant cell lines in the molecular weight range between 14 and about 35 kDa. 3. The low-malignant cell lines exhibited a significantly higher surface charge as deduced from their higher sialic acid content compared to the high-malignant variants. 4. They also showed a greater membrane lipid fluidity as determined both by the cholesterol to phospholipid ratio and by fluorescence depolarization measurements.
authors.affiliation: Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg, F.R.G.
authors.forename: E W
authors.initials: EW
authors.lastname: Haeffner
authors.forename: K H
authors.initials: KH
authors.lastname: Doenges
authors.forename: J
authors.initials: J
authors.lastname: Buchholz
mesh_terms: D015720:5'-Nucleotidase; D000230:Adenocarcinoma / Q000201:enzymology*; D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D007455:Iodine / Q000378:metabolism*; D008223:Lymphoma / Q000201:enzymology*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D009708:Nucleotidases / Q000378:metabolism*; D051381:Rats
chemical_list: D005227:Fatty Acids; D008563:Membrane Lipids; D007455:Iodine; D009708:Nucleotidases; D015720:5'-Nucleotidase