Extraction and partial characterization of surfactant-soluble antigens from adult female Dirofilaria immitis.
Antigens were solubilized from aqueous-insoluble material of adult female Dirofilaria immitis with Triton X-100 and sodium dodecyl sulfate (SDS) and partially characterized. Concentrations of 2.1-8.3 mg Triton X-100/mg total protein and 1.06-4.26 mg SDS/mg total protein were used to solubilize antigens. Triton X-100 was more efficient in solubilizing protein than SDS; Triton X-100 and SDS released 75% and 26% of the available protein, respectively. Protein mixtures solubilized with different levels of Triton X-100 appeared to be identical in immunodiffusion. Crossed immunoelectrophoresis revealed six antigen systems. Triton X-100 solubilized antigens did not react in immunodiffusion with sera from 7 D. immitis-infected, microfilaremic dogs, but did react with sera from 4 of 5 dogs with occult D. immitis infections.
Grieve
R B
RB
DeGregory
K T
KT
Lindmark
D G
DG
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D003902:Detergents; D004183:Dirofilaria immitis / Q000276:immunology*; D004285:Dogs; D005260:Female; D005370:Filarioidea / Q000276:immunology*; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D017830:Octoxynol; D011092:Polyethylene Glycols; D011233:Precipitin Tests; D012967:Sodium Dodecyl Sulfate
D000947:Antigens, Helminth; D003902:Detergents; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D017830:Octoxynol