title: Effects of detergent on the binding of solubilized sodium channels to immobilized wheat germ agglutinin: structural implications.
abstract: The binding of the solubilized voltage-dependent sodium channel from rat brain to immobilized wheat germ agglutinin (WGA) is detergent-dependent. When solubilized in sodium cholate, only 11% of total recovered channels bound to a WGA-Sepharose column. When solubilized in Triton X-100 or CHAPS, however, 80% and 60% could bind, respectively. The effect of cholate on sodium channel binding is relatively specific: the major rat brain glycoproteins which bind to immobilized WGA are roughly the same in either Triton or cholate, as analyzed by SDS gel electrophoresis. The structural implications for the channel are discussed.
authors.affiliation: Department of Physiology and Biophysics, New York University Medical Center, New York 10016.
authors.forename: J S
authors.initials: JS
authors.lastname: Weiner
authors.forename: B
authors.initials: B
authors.lastname: Rudy
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002458:Cell Fractionation / Q000379:methods; D003902:Detergents / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation; D051381:Rats; D012530:Saxitoxin / Q000378:metabolism; D015222:Sodium Channels / Q000378:metabolism*; D012995:Solubility; D013501:Surface-Active Agents / Q000494:pharmacology*; D014909:Wheat Germ Agglutinins / Q000378:metabolism*
chemical_list: D003902:Detergents; D008562:Membrane Glycoproteins; D015222:Sodium Channels; D013501:Surface-Active Agents; D014909:Wheat Germ Agglutinins; D012530:Saxitoxin