Calculation of individual cleavage rates from partial digests in restriction endonuclease kinetics.
A correction for results obtained by an analysis of DNA molecules partially cleaved with restriction endonuclease was suggested. The correction was proved on model data. Applications to (i) electron-microscopic analysis of singly cleaved molecules, (ii) partial digestion of a circular molecule followed by complete digestion with a second enzyme, (iii) systems with great cleavage differences, and (iv) partial cleavage of end-labelled molecules were discussed.
Karlovský
P
P
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
D010582:Bacteriophage lambda; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004270:DNA, Circular / Q000378:metabolism; D004279:DNA, Viral / Q000648:ultrastructure; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D007700:Kinetics; D008854:Microscopy, Electron; D008954:Models, Biological
D004270:DNA, Circular; D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI