Murine responses to poly (Phe-Glu-Ala-Gly) and poly (Phe-Ala-Glu-Gly): comparisons between the specificities of the T-cell and B-cell responses.
The murine immune response patterns to (Phe-Glu-Ala-Gly)n and (Phe-Ala-Glu-Gly)n differ from those of the tyrosine analogues (Tyr-Glu-Ala-Gly)n and (Tyr-Ala-Glu-Gly)n. (Phe-Glu-Ala-Gly)n was not immunogenetic in inbred, congenic or recombinant strains of mice. (Phe-Ala-Glu-Gly)n was immunogenic only in mice having f alleles in the IA subregion of the H-2 complex. Reciprocal in vitro cross reactions were noted with T cells from mice of H-2f that respond to either (Phe-Ala-Glu-Gly)n or (Tyr-Ala-Glu-Gly)n. T cells from mice of H-2b, which are responders to (Tyr-Glu-Ala-Gly)n, could not be stimulated with the nonimmunogenic (Phe-Glu-Ala-Gly)n. These results support other studies showing that for any polymer to elicit cross T-cell proliferative responses it must be (a) structurally related to the homologous polymer and (b) immunogenic in the mouse strain whose T cells are being challenged in vitro. Explanations are offered for these statements.
Maurer
P H
PH
Department of Biochemistry, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa.
Lai
C H
CH
Zeiger
A R
AR
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D006183:H-2 Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011108:Polymers; D013601:T-Lymphocytes / Q000276:immunology*
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D010455:Peptides; D011108:Polymers; C064213:poly(phenylalanyl-alanyl-glutamyl-glycine); C051032:poly(tyrosyl-glutamyl-alanyl-glycyl); C064212:poly(phenylalanyl-glutamyl-alanyl-glycine); C064211:poly(tyrosyl-alanyl-glutamyl-glycine)