Effects of immunization with bovine herpesvirus-1 glycoproteins on bovine herpesvirus-1-induced alteration of bovine neutrophil chemotactic and anti-Pasteurella haemolytica activities.
It has been reported previously that active bovine herpesvirus-1 (BHV-1) infection greatly enhances the susceptibility of cattle to secondary bacterial pneumonia involving Pasteurella haemolytica. The present study examines the possibility that immunization of BHV-1 naive calves with purified BHV-1 glycoproteins would protect them against changes in neutrophil function that might compromise their ability to eliminate P. haemolytica during an active BHV-1 infection. The results show that circulating neutrophil chemotactic activity was generally reduced at 7-8 days after BHV-1 challenge; immunization with a 77 kilodalton BHV-1 glycoprotein (gIV) prevented impairment of neutrophil chemotaxis. BHV-1 infection did not markedly affect the ability of neutrophils to ingest and kill P. haemolytica in vitro. Immunization and challenge with BHV-1 had little effect on the chemiluminescence response of bovine neutrophils to opsonized P. haemolytica in vitro, although in one experiment a marked increase in baseline neutrophil chemiluminescence was observed which may be relevant to understanding the pathogenesis of pulmonary damage that occurs in BHV-1 infected calves.
Noel
E J
EJ
Department of Pathobiological Sciences, School of Veterinary Medicine, Madison, WI 53706.
Israel
B A
BA
Letchworth
G J
GJ
Czuprynski
C J
CJ
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001770:Blood Bactericidal Activity; D002417:Cattle; D002634:Chemotaxis, Leukocyte; D004268:DNA-Binding Proteins; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007241:Infectious Bovine Rhinotracheitis / Q000097:blood / Q000276:immunology / Q000382:microbiology; D007958:Leukocyte Count; D008163:Luminescent Measurements; D009504:Neutrophils / Q000502:physiology*; D010326:Pasteurella Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D014764:Viral Proteins / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D004268:DNA-Binding Proteins; C045462:ICP8 protein, Simplexvirus; D014764:Viral Proteins