title: The isolation of a toxin from the dahlia sea anemone, Tealia felina L.
abstract: Gel chromatography with Dyematrex blue B gel and ion exchange chromatography with a methylcellulose cation exchange column have been used to isolate a toxin present in partially purified saline extracts of the dahlia sea anemone Tealia felina. Toxin activity was assessed by its histaminolytic activity. A ten-fold increase in activity per mg protein was achieved in these two steps. Final purification on a Sephacryl column yielded an apparently pure protein with a molecular weight of 7800. The amino acid composition of this compound was determined.
authors.forename: R C
authors.initials: RC
authors.lastname: Elliott
authors.forename: R S
authors.initials: RS
authors.lastname: Konya
authors.forename: K
authors.initials: K
authors.lastname: Vickneshwara
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003063:Cnidaria / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008387:Marine Toxins / Q000302:isolation & purification*; D008970:Molecular Weight; D012615:Sea Anemones / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000596:Amino Acids; D008387:Marine Toxins