Release of cell wall peptides into culture medium by exponentially growing Escherichia coli.
Escherichia coli W7 cells were found to release three different muropeptides into the culture medium: tetrapeptide (L-Ala-D-Glu-meso-diaminopimelic acid-D-Ala), tripeptide (L-Ala-D-Glu-meso-diaminopimelic acid), and a previously undescribed dipeptide (meso-diaminopimelic acid-D-Ala). From the rate of release of these three peptides, it was calculated that 6 to 8% of the murein in the sacculus was lost per generation.
Goodell
E W
EW
Schwarz
U
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D002470:Cell Survival; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000032:analysis; D009842:Oligopeptides / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism*; D014316:Tritium
D003470:Culture Media; D009842:Oligopeptides; D010457:Peptidoglycan; D014316:Tritium; D009238:N-Acetylmuramoyl-L-alanine Amidase