Non-neurovirulent, genetically stable clone of poliovirus (Sabin type III vaccine strain).
A clone of poliovirus type III (TRU-3) was derived from the Sabin-3 vaccine strain by the cDNA:RNA hybrid molecular cloning method. The virus recovered was found to be considerably less neurovirulent for primates than its progenitor Sabin III vaccine strain. Upon serial passages in humans, TRU-3 did not increase its neurovirulence (unlike the Sabin strain) and maintained the base sequence of the attenuated strain in a critical part of the 5' region of the genome (unlike the Sabin strain). The substitution of TRU-3 clone for Sabin type III in oral polio vaccine could eliminate the vast majority of cases of vaccine-associated poliomyelitis.
Ubertini
T R
TR
Sclavo Biopharmaceuticals, Siena, Italy.
Cioni
C
C
Perini
L
L
Gagnoni
S
S
D000818:Animals; D001483:Base Sequence; D006801:Humans; D017955:Poliovirus / Q000302:isolation & purification / Q000472:pathogenicity*; D011055:Poliovirus Vaccine, Oral / Q000235:genetics*; D011995:Recombination, Genetic; D014774:Virulence
D011055:Poliovirus Vaccine, Oral