[Temporal distribution of electrophoretic profiles of nucleic acid from rotavirus in the feces of children in Belém, Pará, Brazil].
Electrophoresis of rotavirus RNA in polyacrylamide gel was carried out by using faecal samples collected from children living in the peripheral area of Belém, Brazil. Typical rotavirus profiles (eleven bands) were noted in 46(76.7%) out of 60 ELISA rotavirus positive specimens. According to the electrophoretic patterns, 5(10.9%) were classified as subgroup I, while 41(89.1%) of them as subgroup II. Lacking of bands 10 and 11 in 14(23.3%) samples did not allow us to classify their electropherotypes. The profile coded as "1N2L" was the predominant one, accounting for 65.2% of the classified strains.
Mascarenhas
J D
JD
Instituto Evandro Chagas, Fundação Serviços de Saúde Pública, Belém, PA, Brasil.
Gabbay
Y B
YB
Freitas
R B
RB
Linhares
A C
AC
D000956:Antigens, Viral / Q000032:analysis; D001938:Brazil; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D005814:Genes, Viral; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology
D000956:Antigens, Viral; D012367:RNA, Viral
Distribuição temporal de perfis eletroforéticos de ácido nucleico de rotavírus em fezes de crianças em Belém, Pará.