Comparison of the intravenous and intraperitoneal routes of administration of tritiated thymidine in studies of cell production in the gastrointestinal tract of the rat.
Incorporation of 3H-Thymidine into DNA-synthesizing cells of the gastrointestinal tract of the rat was examined following administration of the isotope by intraperitoneal and intravenous routes. Estimates of whole tissue incorporation expressed as DPM/mg dry weight and of proliferating cells expressed as DPM/crypt or gland in the different segments of the gut indicated no differences in the degree of 3H-TdR uptake into DNA following intraperitoneal or intravenous routes of administration. The possibility of misdirected I.P. injections was examined following injection of 3H-TdR into the cecum or bladder. DPM/mg wet weight of gastrointestinal tissues indicated reduction in the uptake of 3H-TdR into DNA of intestinal tissues following intracecal and intrabladder administration of 3H-Tdr. The intraperitoneal route of administration of 3H-TdR appears to be equally effective in the distribution of the isotope into different segments of the gut when compared to the intravenous route and is a more convenient method in studies of cell production in the gastrointestinal tract of the rat.
Fatemi
S H
SH
Cullan
G E
GE
Cullan
G M
GM
Sharp
J G
JG
D000818:Animals; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis*; D004064:Digestive System / Q000166:cytology*; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D013936:Thymidine / Q000008:administration & dosage; D014316:Tritium / Q000008:administration & dosage
D014316:Tritium; D004247:DNA; D013936:Thymidine