Improved expression of streptomycin resistance in plants due to a deletion in the streptomycin phosphotransferase coding sequence.
Previous studies have shown that a chimeric streptomycin phosphotransferase (SPT) gene can function as a dominant marker for plant cell transformation. The SPT marker previously described by Jones and co-workers has a limited value since it conferred a useful level of resistance only to a fraction (10%) of Nicotiana plumbaginifolia transgenic lines. Expression of resistance was species specific: no such resistant transformants were found in N. tabacum. In this paper we describe an improved SPT construct that utilizes a mutant Tn5 SPT gene. The mutant gene, SPT*, encodes a protein with a two amino acid deletion close to its COOH-terminus. In N. tabacum cell culture the efficiency of transformation with the improved streptomycin resistance marker was comparable to kanamycin resistance. When the chimeric SPT* gene was introduced linked to a kanamycin resistance gene, streptomycin resistance was expressed in most of the transgenic N. tabacum lines.
Maliga
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P
Advanced Genetic Sciences Inc., Oakland, CA 94608.
Svab
Z
Z
Harper
E C
EC
Jones
J D
JD
D002678:Chimera; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005819:Genetic Markers; D005822:Genetic Vectors; D007613:Kanamycin Resistance / Q000235:genetics; D009154:Mutation; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010947:Plants, Toxic; D010957:Plasmids; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D005819:Genetic Markers; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022679:streptomycin 3''-kinase