Detection of novel non-ras oncogenes in rat nasal squamous cell carcinomas.
Rat nasal squamous cell carcinomas induced by inhalation of three direct-acting alkylating agents yielded DNA containing activated oncogenes with no homology to any member of the ras family. The novel NIH 3T3 transforming oncogenes from tumors induced by beta-propiolactone and methylmethane sulfonate are distinct from each other based on restriction analysis. The gene isolated from beta-propiolactone-induced tumors is between 6 and 9 kb in size. None of the tumors induced by dimethylcarbamyl chloride contained positive DNA in the NIH 3T3 focus assay or in the nude mouse cotransfection assay. The rat nasal tumor model is apparently ideally suited for analysis of the roles of carcinogen and tissue specificity in oncogene activation, especially related to novel (non-ras) transforming oncogenes.
Hochwalt
A E
AE
Department of Environmental Medicine, New York University Medical Center, New York 10016.
Wirgin
I
I
Felber
M
M
Currie
D D
DD
Garte
S J
SJ
D000818:Animals; D015139:Blotting, Southern; D002219:Carbamates / Q000633:toxicity; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009303:Nasopharyngeal Neoplasms / Q000139:chemically induced / Q000235:genetics*; D009857:Oncogenes / Q000187:drug effects; D011420:Propiolactone / Q000633:toxicity; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D051381:Rats; D014162:Transfection
D002219:Carbamates; D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; C001036:dimethylcarbamyl chloride; D011420:Propiolactone; D008741:Methyl Methanesulfonate; D004262:DNA Restriction Enzymes; D016283:Proto-Oncogene Proteins p21(ras)