Molecular pathogenesis of equine coital exanthema: identification and expression of infected cell polypeptides at the restricted temperature during equine herpesvirus 3 infection.
Equine herpesvirus 3 (EHV-3)-infected equine cells display a kinetics of infected cell polypeptide (ICP) synthesis at 34 degrees C that is typical of coordinate cascade gene regulation of herpesviruses. In contrast, when infected cell cultures are incubated at the restricted temperature of 39 degrees C, the shift from early (beta) gene expression to late (gamma) gene expression is perturbed, i.e., there is an accumulation of early (beta) gene products and a decrease in, or absence of, late (gamma) gene products. Some of the affected late (gamma) gene products may be glycoproteins since these ICPs co-migrated with radiolabeled bands from infected cells incubated with [3H] glucosamine, separated by polyacrylamide gel electrophoresis. These findings are consistent with previous findings (Jacob, 1986), indicating that the growth restriction is in a late viral function(s) and possibly involves envelopment of nucleocapsids into infectious virions.
Jacob
R J
RJ
Department of Microbiology and Immunology, Chandler Medical Center, Lexington, KY.
Steiner
M R
MR
D000818:Animals; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D004858:Herpesvirus 3, Equid / Q000235:genetics* / Q000302:isolation & purification; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D010455:Peptides / Q000032:analysis* / Q000235:genetics; D013462:Sulfur Radioisotopes; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
D010455:Peptides; D013462:Sulfur Radioisotopes; D014764:Viral Proteins