Opioid peptides increase calcium uptake by synaptosomes from brain regions.
The fast-phase calcium uptake by depolarized hippocampal synaptosomes was increased significantly following treatment with 10 nM or 10 microM D-Ala2,D-Leu5-enkephalin (DADLE). No significant changes of calcium uptake by depolarized cortical or striatal synaptosomes were observed for 10 nM or 10 microM treatment with this enkephalin analog. Dynorphin 1-17 analog at 10 nM produces a significant increase in calcium uptake by depolarized striatal synaptosomes. beta-Endorphin and the dynorphin 1-13 fragment analog (10 nM) caused no significant change in the calcium uptake by depolarized synaptosomes from any of the three brain regions. However, calcium uptake by non-depolarized striatal and cortical synaptosomes was increased significantly in the presence of 10 nM beta-endorphin and DADLE. Opioid peptide action on neural calcium uptake is complex and appears to vary somewhat from one brain region to another.
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D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism; D004399:Dynorphins / Q000494:pharmacology; D004723:Endorphins / Q000494:pharmacology*; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D016308:Enkephalin, Leucine-2-Alanine; D005625:Frontal Lobe / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D066298:In Vitro Techniques; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism; D001615:beta-Endorphin
D004723:Endorphins; D010446:Peptide Fragments; D004743:Enkephalin, Leucine; D001615:beta-Endorphin; D016308:Enkephalin, Leucine-2-Alanine; C026843:dynorphin (1-13); D004399:Dynorphins; D002118:Calcium