Modified spin-amplified adsorption procedure with conventional tissue culture tubes for rapid detection and increased recovery of herpes simplex virus from clinical specimens.
Conventional culture tubes were used in a modification of the spin-amplified adsorption procedure for recovery of herpes simplex virus (HSV) from clinical specimens. The sensitivity of isolation of HSV from 864 specimens adsorbed by the spin-amplified method was 100% (127 of 127), compared with 88.2% (112 of 127) for stationary-phase-adsorbed specimens. Cytopathic effect developed more rapidly in 32.1% (36 of 112) of isolates adsorbed by spin amplification than in those adsorbed by stationary means. In a separate quantitative study, cultures of HSV type 1 adsorbed by spin amplification yielded higher antigen levels and greater cytopathic effect than stationary-phase-adsorbed cultures. Cells grown in conventional tissue culture tubes may be used in a spin-amplified adsorption for rapid detection and increased sensitivity of HSV isolation.
Oefinger
P E
PE
Department of Pathology, University of Texas Medical School, Houston 77030.
Loo
S H
SH
Gander
R M
RM
D000327:Adsorption; D000956:Antigens, Viral / Q000032:analysis; D002498:Centrifugation; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018139:Simplexvirus / Q000276:immunology / Q000302:isolation & purification*
D000956:Antigens, Viral