Activation of phosphatidic acid metabolism of human erythrocyte membranes by perfringolysin O.
The effect of perfringolysin O on the lipid metabolism of human erythrocyte membranes was investigated. Erythrocytes were prelabeled with [3H]arachidonic acid and [32P]inorganic phosphate. In the presence of calcium ion(5.5 mM), the effect of perfringolysin O on lipid metabolism was very similar to that of an calcium-ionophore A23187. In the absence of calcium ion, the accumulation of phosphatidic acid and its following decreasing trend were observed during the reaction with the toxin. Such changes were not caused by filipin. These results suggest that perfringolysin O causes the activation of a diglyceride-phosphatidic acid cycle, which might be involved in the calcium transport.
Saito
M
M
Ando
S
S
Mitsui
K
K
Homma
Y
Y
Takenawa
T
T
D001427:Bacterial Toxins / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D003016:Clostridium perfringens; D004075:Diglycerides / Q000097:blood; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010712:Phosphatidic Acids / Q000097:blood*
D001427:Bacterial Toxins; D004075:Diglycerides; D006460:Hemolysin Proteins; D010712:Phosphatidic Acids; D000001:Calcimycin; C020452:Clostridium perfringens theta-toxin; D002118:Calcium