Decrease of benzodiazepine receptors in frontal cortex of alcoholics.
We hypothesize that chronic alcohol abuse results in a loss of neurons and their associated synaptic receptors. This encephalopathy may be a precursor of brain atrophy and end-stage dementia. Autopsies were performed on normal brains of 27 alcoholics (mean age 62.5) and 30 nonalcoholic matched controls (mean age 64.4) free of other brain and liver diseases. None had recently received benzodiazepine medications. Gross brain atrophy was slight and equal in both groups. Benzodiazepine receptor densities and affinities in homogenates of frontal cortex were determined using [3H]flunitrazepam. Bmax specific binding was reduced by 20% in alcoholics compared with nonalcoholic controls of comparable age and with similar death-autopsy time intervals. The affinity was slightly less in the alcoholics. Wet tissue brain protein concentrations and their yields of 50,000-g pellet proteins were similar. Aging, death-autopsy time intervals, pneumonia and chronic obstructive pulmonary disease (diseases usually associated with hypoxia) had no significant effect on brain proteins, receptor densities, or affinities. We conclude that chronic alcoholism is associated with a loss of benzodiazepine receptor densities. Alcohol abuse may affect the results of post-mortem neurochemical investigations of other diseases.
Freund
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Veterans Administration Medical Center, Gainesville, FL 32602.
Ballinger
W E
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D000437:Alcoholism / Q000209:etiology / Q000378:metabolism*; D001344:Autopsy; D003627:Data Interpretation, Statistical; D000431:Ethanol / Q000009:adverse effects; D005445:Flunitrazepam / Q000494:pharmacology; D005625:Frontal Lobe / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008875:Middle Aged; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism
D011963:Receptors, GABA-A; D017981:Receptors, Neurotransmitter; D000431:Ethanol; D005445:Flunitrazepam