A novel bioaction of PAF: induction of microbicidal activity in guinea pig bone marrow cells.
When guinea pig bone marrow cells were incubated in the presence of 10(-8) to 10(-6) M platelet activating factor (PAF) for 24 to 72 hr, microbicidal activity against Candida parapsilosis of cells was augmented. This augmentation was inhibited by PAF-specific antagonists, CV6209 or FR900452. PAF-specific binding sites with a high affinity were found on guinea pig bone marrow cells. Carrageenan or 2-chloroadenosine, reagents known to be preferentially cytotoxic to macrophages, abolished the microbicidal activity of PAF-treated bone marrow cells. Macrophages prepared from the peritoneal cavity, however, acquired no appreciable microbicidal action by treatment with PAF. These observations suggest that PAF may affect a class of guinea pig bone marrow cells through binding to receptors specific to PAF, resulting in activation and/or induction of differentiation of monocyte-macrophage lineage cells.
Hayashi
H
H
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Kudo
I
I
Kato
T
T
Nozawa
R
R
Nojima
S
S
Inoue
K
K
D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000382:microbiology; D001854:Bone Marrow Cells; D002175:Candida / Q000187:drug effects*; D002351:Carrageenan / Q000494:pharmacology; D002478:Cells, Cultured; D006168:Guinea Pigs; D010972:Platelet Activating Factor / Q000494:pharmacology*; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface / Q000187:drug effects; D043562:Receptors, G-Protein-Coupled
D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D015762:2-Chloroadenosine; D002351:Carrageenan; D000241:Adenosine