Determination of base composition of DNA by high performance liquid chromatography of its nuclease P1 hydrolysate.
The base composition of DNA was directly determined by high performance liquid chromatography (HPLC) of its nuclease P1 hydrolysate. This method can satisfactorily be employed even if the sample of DNA contains RNA or the amount of the sample is very small.
Kumagai
M
M
Research Laboratories, Yamasa Shoyu Co., Ltd., Choshi, Japan.
Fujimoto
M
M
Kuninaka
A
A
D001482:Base Composition; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA; D006868:Hydrolysis; D009708:Nucleotidases; D015719:Single-Strand Specific DNA and RNA Endonucleases
D004247:DNA; D009708:Nucleotidases; D015719:Single-Strand Specific DNA and RNA Endonucleases