title: Studies of proteins soluble in acidic chloroform-methanol isolated from crude human leukocyte interferon preparations.
abstract: Acidic chloroform-methanol soluble proteins possessing hydrophobic properties and capable of inhibiting in vitro transcriptase activity of influenza virus RNP were detected in native and partially purified human leukocyte interferon (IFN) preparations. Purification of IFN resulted in the removal of at least a portion of such proteins; however, no proteins have been found in highly-purified IFN preparations.
authors.forename: I A
authors.initials: IA
authors.lastname: Leneva
authors.forename: A V
authors.initials: AV
authors.lastname: Mikhejeva
authors.forename: V P
authors.initials: VP
authors.lastname: Kuznetsov
authors.forename: W
authors.initials: W
authors.lastname: Seidel
authors.lastname: Ghendon YuZ
mesh_terms: D002725:Chloroform; D003588:Cytopathogenic Effect, Viral; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000201:enzymology; D007370:Interferon Type I / Q000032:analysis* / Q000302:isolation & purification; D000432:Methanol; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D012261:Ribonucleoproteins / Q000378:metabolism; D012995:Solubility
chemical_list: D007370:Interferon Type I; D011506:Proteins; D012261:Ribonucleoproteins; D002725:Chloroform; D012321:DNA-Directed RNA Polymerases; D000432:Methanol