Translation of muscle mRNA in rats following acute exposure to Pb2+ or Cd2+.
The hindleg muscle of rats was studied 2 days following the i.p. administration of 0.5 mg Pb2+ /100 g body wt or 0.12 mg Cd2+ /100 g body wt or both Pb2+ and Cd2+. The incorporation of [14C]leucine into proteins was measured using mRNA obtained from the muscle polysomes. The translatability of this poly(A)+ RNA in a mRNA-dependent reticulocyte lysate was elevated similarly in each of the preparations from heavy metal treated rats compared to control rats. Evidence for increased mRNA activity for glyceraldehyde-3-phosphate dehydrogenase and actin was obtained.
Nicholls
D M
DM
Teichert-Kuliszewska
K
K
Kuliszewski
M J
MJ
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007525:Isoelectric Focusing; D007854:Lead / Q000633:toxicity*; D008297:Male; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism*; D011233:Precipitin Tests; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000378:metabolism
D012333:RNA, Messenger; D002104:Cadmium; D007854:Lead; D005954:Glucosephosphate Dehydrogenase