title: Leishmania mexicana amazonensis: heterogeneity in 5'-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection.
abstract: The degree of maturation of cells of the Mononuclear Phagocyte System (MPS), during in vivo and in vitro infection by Leishmania mexicana amazonensis, was evaluated in this study. The macrophages' differentiation was assayed by cytochemical characterization at the ultrastructural level, using two well-established markers: 5'-nucleotidase enzyme activity, for revealing the mature cells; and the peroxidase activity present in the cell granules to demonstrate immature mononuclear phagocytes. Only a few macrophages, demonstrating 5'-nucleotidase positive reaction in both the plasma membrane and within their cytoplasmic vesicles, were found scattered in the chronic inflammation at the L. m. amazonensis lesions in albino mice. However, by the peroxidase activity analysis, we were also able to demonstrate the presence of immature MPS cells, which predominate, together with parasitized vacuolated macrophages, in chronic lesions induced in this system by L. m. amazonensis. The implications of these results on the pathogenesis of murine cutaneous leishmaniasis are discussed.
authors.affiliation: Instituto Oswaldo Cruz, Departamento de Ultraestrutura e Biologia Celular, Rio de Janeiro, Brasil.
authors.forename: S
authors.initials: S
authors.lastname: Côrte-Real
authors.forename: G
authors.initials: G
authors.lastname: Grimaldi Juńior
authors.forename: M de N
authors.initials: Mde N
authors.lastname: De Meirelles
mesh_terms: D015720:5'-Nucleotidase; D000818:Animals; D002454:Cell Differentiation; D005260:Female; D007896:Leishmaniasis / Q000201:enzymology* / Q000473:pathology; D008264:Macrophages / Q000201:enzymology* / Q000648:ultrastructure; D051379:Mice; D009708:Nucleotidases / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D010586:Phagocytes / Q000201:enzymology*
chemical_list: D010544:Peroxidases; D009708:Nucleotidases; D015720:5'-Nucleotidase