Antiviral and antigenic properties of recombinant porcine interferon gamma.
Recombinant porcine interferon gamma (rPoIFN gamma) induced a dose-dependent inhibition of the cytopathic effect produced by vesicular stomatitis virus (VSV) challenge of both homologous and heterologous (bovine) cell lines. In addition, an antiviral effect of rPoIFN gamma was demonstrable against the coronavirus transmissible gastroenteritis virus (TGEV) infection of porcine epithelial cells and of pulmonary macrophages. A rabbit anti-PoIFN gamma antiserum was prepared and shown to specifically neutralize the antiviral effects of natural and recombinant porcine IFN gamma preparations. This antiserum could also neutralize recombinant bovine IFN gamma but not recombinant human IFN gamma. These results suggest antigenic homology of porcine and bovine IFN gamma but antigenic differences between these molecules and human IFN gamma.
Charley
B
B
INRA, Station de Recherches de Virologie et d'Immunologie, Jouy-en-Josas, France.
McCullough
K
K
Martinod
S
S
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D003332:Coronaviridae / Q000276:immunology*; D003588:Cytopathogenic Effect, Viral; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007370:Interferon Type I / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology*; D008264:Macrophages / Q000382:microbiology; D009500:Neutralization Tests; D011994:Recombinant Proteins; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*
D007370:Interferon Type I; D011994:Recombinant Proteins; D007371:Interferon-gamma