title: [A method for inducing the point C----T transitions in DNA sequences corresponding to the ends of restriction sites].
abstract: A new method for inducing of C----T substitutions into cytosine-containing restriction sites is developed. The method, based on the selective modification of cytosine residues in DNA sticky ends by sodium bisulfite, was illustrated by induction of a base substitution (C----T at the BamHI site of pBR322 plasmid DNA.
authors.forename: O A
authors.initials: OA
authors.lastname: Medvedev
authors.forename: T V
authors.initials: TV
authors.lastname: Timchenko
authors.forename: G L
authors.initials: GL
authors.lastname: Dianov
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes
vernacular_title: Metod polucheniia tochkovykh C----T tranzitsiĭ po uchastkam DNK, sootvetstvuiushchim kontsam saĭtov restriktsii.