Phosphoinositide breakdown and superoxide anion release in formyl-peptide-stimulated human alveolar macrophages. Comparison between quiescent and activated cells.
Human alveolar macrophages (AMs) from allergic asthmatics (AAs) showed continuous Li+-sensitive production of IP1, indicating that the cells were continuously activated. Furthermore, whereas the accumulation of IP1, IP2 and IP3 rapidly increased by as much as 125-175% in chemotactic-factor-stimulated AMs from healthy subjects, stimulation of cells from AAs increased these inositol phosphates only slightly. This moderate production could be due to a permanent state of activation leading to a depleted pool of polyphosphoinositides, corroborating the greater capacity of these cells to generate superoxide anion after stimulation by a chemoattractant. The activation state could be due to the action of priming agents, which are known to be released into the inflammatory sites.
Damon
M
M
INSERM, U58, Montpellier, France.
Vial
H
H
Crastes de Paulet
A
A
Godard
P
P
D002478:Cells, Cultured; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine