title: A physical map of the human pseudoautosomal region.
abstract: A physical map of the human pseudoautosomal region has been constructed using pulsed field gel electrophoresis and the infrequently cutting restriction enzymes BssHIII, EagI, SstII, NotI, MluI and NruI. This map extends 2.3 Mbp from the telomere to sex-chromosome-specific DNA, includes at least seven CpG islands and locates four genetically mapped loci. Five of the CpG islands are organized into two clusters. One cluster is adjacent to the telomere, the other extends into sex-chromosome-specific DNA. There is congruence between the genetic and physical maps which implies that the frequency of recombination is approximately uniform throughout the DNA.
authors.affiliation: Biochemistry Department, Oxford University, UK.
authors.forename: W R
authors.initials: WR
authors.lastname: Brown
mesh_terms: D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D012730:Sex Chromosomes; D014424:Turner Syndrome / Q000235:genetics
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes