Incorporation and toxicity of 32P-orthophosphate and occurrence of polyphosphate in Entamoeba trophozoites.
We explored the requirements of inorganic phosphate (Pi), the incorporation of 32P-orthophosphate (32Pi), and the occurrence of inorganic polyphosphate (polyP) in axenic Entamoeba cultures. Maximal population densities and growth rates of Entamoeba histolytica trophozoites were attained in complete TP-S-1 medium. As 32Pi concentration was increased in the medium, its own incorporation and the culture growth rate were progressively inhibited, especially in Pi-deficient medium. PolyP grains were found in the cytoplasm and occasionally in the nuclear membrane of E. histolytica-like, E. invadens, and E. moshkovskii trophozoites.
López-Revilla
R
R
Gómez-Domínguez
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R
D000818:Animals; D002845:Chromatography; D003470:Culture Media; D003593:Cytoplasm / Q000032:analysis; D004747:Entamoeba / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D009685:Nuclear Envelope / Q000032:analysis; D010710:Phosphates / Q000378:metabolism* / Q000494:pharmacology; D011122:Polyphosphates / Q000032:analysis*
D003470:Culture Media; D010710:Phosphates; D011122:Polyphosphates