title: The effect of cAMP on ion transport in the proximal tubular cells in bullfrog kidney.
abstract: Using a direct cellular micropuncture technique with double-barreled ion-selective microelectrodes, we investigated the effect of dibutyryl-cyclic AMP (db-cAMP) on the membrane potential and the transport of Na+, K+, and H+ in doubly-perfused bullfrog proximal tubules. The peritubular membrane potential difference (EM) and the intracellular K+, Na+ activities ((K)i, (Na)i) or intracellular and luminal pH were monitored continuously after peritubular administration of db-cAMP (10(-3)-10(-4)M).
  
  
  RESULTS
  1) db-cAMP hyperpolarized the EM by 8.0 mV with an increase of (K)i by 4.8 mEq/l; 2) the peritubular administration of high K+ (13.5 and 50 mM) solutions depolarized the EM by 11.5 and 41.5 mV, respectively. The high K+ perfusate with db-cAMP produced a depolarization to the same level as that in simple high K+ perfusion without db-cAMP; 3) db-cAMP transiently blocked the luminal acidification concomitantly with a cellular alkalinization by about 0.1 pH; and 4) db-cAMP caused a decrease of (Na)i by 5.0 mEq/l.
  
  
  CONCLUSIONS
  1) cAMP causes an increase of K+ permeability in the peritubular membrane; 2) cAMP induces a cytosol alkalosis by enhancing incorporation of H+ into the subcellular vesicles, thus favoring the activation of Na+/K+ pump; and 3) cAMP, in effect, suppresses the Na+/H+ exchange mechanism in the luminal membrane and transiently blocks the proximal urinary acidification.
authors.affiliation: Department of Physiology, Osaka Medical College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fujimoto
authors.forename: N
authors.initials: N
authors.lastname: Hagiwara
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: K
authors.initials: K
authors.lastname: Kotera
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D011188:Potassium / Q000493:pharmacokinetics*; D011892:Rana catesbeiana; D012964:Sodium / Q000493:pharmacokinetics*
chemical_list: D003994:Bucladesine; D012964:Sodium; D011188:Potassium