Purification and characterization of an antibacterial factor from snail mucus.
The antibacterial factor from the body surface of the African giant snail, Achatina fulica Férussac, was isolated by DEAE-Toyopearl 650M ion exchange chromatography. The isolated preparation exhibited highly positive antibacterial activity both for the Gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus and for the Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, but it lost such activity when heated at 75 degrees C for 5 min. The antibacterial factor of the snail mucus was a glycoprotein whose molecular weight (MW) was about 160,000. It was composed of two subunits of MW 70,000-80,000.
Kubota
Y
Y
Watanabe
Y
Y
Otsuka
H
H
Tamiya
T
T
Tsuchiya
T
T
Matsumoto
J J
JJ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009093:Mucus / Q000032:analysis*; D012908:Snails; D013045:Species Specificity
D000900:Anti-Bacterial Agents