The DNA-binding properties of immediate-early and early proteins of murine cytomegalovirus.
The DNA-binding properties of the immediate early (IE) and early proteins induced by the murine cytomegalovirus (Smith strain) in permissively infected 3T3-L 1 cells and nonpermissively infected J774A.1 cells were characterized. Eight of 10 IE proteins and 4 of 7 early proteins had affinity for DNA (native or denatured). A 96K IE protein was observed to have affinity for denatured DNA-cellulose when synthesized in J774A.1 cells but not when synthesized 3T3-L 1 cells.
Walker
D G
DG
Division of Medical Microbiology, University of British Columbia, Vancouver, Canada.
Hudson
J B
JB
D000956:Antigens, Viral / Q000302:isolation & purification / Q000378:metabolism*; D001345:Autoradiography; D002846:Chromatography, Affinity; D003587:Cytomegalovirus / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005374:Filtration; D017874:Immediate-Early Proteins; D014776:Virus Cultivation
D000956:Antigens, Viral; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus