Untargeted viral mutagenesis is not found in X-irradiated monkey cells.
The existence of untargeted viral mutagenesis in X-irradiated cells was investigated in a mammalian virus/cell system, where a low level of such viral mutagenesis can be demonstrated in UV-irradiated cells. In the positive control experiment UV-elicited mutagenesis was shown with cell exposures of 5, 10 and 15 J/m2 and a delay of 24 h between cell irradiation and infection with unirradiated herpes simplex virus. Although X-ray doses of 1, 3 and 10 Gy elicit enhanced reactivation of UV-irradiated virus, no untargeted mutagenesis for any X-ray dose at post-irradiation infection times of 0, 24 or 72 h was observed in this study. Thus untargeted mutagenesis of herpes simplex virus was not demonstrated in X-irradiated monkey cells, under conditions where X-ray-enhanced reactivation occurs and where untargeted mutagenesis in UV-irradiated cells occurs.
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Office of Science and Technology, Center for Devices and Radiological Health, FDA, Rockville, MD 20857.
Carney
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Lee
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Bushar
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D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000528:radiation effects*; D002522:Chlorocebus aethiops; D004307:Dose-Response Relationship, Radiation; D007668:Kidney; D009154:Mutation; D018139:Simplexvirus / Q000235:genetics / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014965:X-Rays