Activation of acetyl-CoA carboxylase. Purification and properties of a Mn2+-dependent phosphatase.
Acetyl-CoA carboxylase was isolated from rat liver by polyethylene glycol precipitation and avidin affinity chromatography. Sodium dodecyl sulfate electrophoresis of the enzyme gives one protein band (Mr 250,000). Phosphate analysis of the carboxylase showed the presence of 8.3 mol of phosphate/mol of subunit (Mr 250,000). The purified carboxylase has low activity in the absence of citrate (specific activity = 0.3 units/mg). However, addition of 10 mM citrate activates the carboxylase 10-fold, with half-maximal activation observed at 2 mM citrate, well above the physiological citrate level. Using this carboxylase as a substrate, we have isolated from rat liver a protein that activates the enzyme about 10-fold. This protein has been purified to near homogeneity (Mr 90,000). Incubation of this protein with 32P-labeled acetyl-CoA carboxylase results in a time-dependent activation of carboxylase with concomitant release of 32Pi, indicating that this protein is a phosphoprotein phosphatase. Both activation and dephosphorylation are dependent on Mn2+, but not citrate. This phosphatase does not hydrolyze p-nitrophenyl phosphate but does show high affinity for acetyl-CoA carboxylase (Km = 0.2 microM) as compared to its action on phosphorylase a (Km = 5.5 microM) and phosphohistone (Km = 20 microM). Activated acetyl-CoA carboxylase was isolated after dephosphorylation by the phosphatase. Such preparations contain about 5 mol of phosphate/mol of subunit and have specific activities of 2.6-3.0 units/mg in the absence of citrate. These activities are comparable to those of the phosphorylated carboxylase in the presence of 10 mM citrate. Thus, dephosphorylation by the Mn2+-dependent phosphatase renders the carboxylase citrate-independent, as compared to the phosphorylated form, which is citrate-dependent. To our knowledge this is the first report of a preparation of animal acetyl-CoA carboxylase that has substantial catalytic activity independent of citrate.
Thampy
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Wakil
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D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D004789:Enzyme Activation / Q000187:drug effects; D008025:Ligases / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D013379:Substrate Specificity
D002951:Citrates; D010710:Phosphates; D019343:Citric Acid; D008345:Manganese; C028732:acetyl CoA carboxylase phosphatase; D010749:Phosphoprotein Phosphatases; D008025:Ligases; D000103:Acetyl-CoA Carboxylase