[Discovery and quantitative determination of intestinal viruses by the detection of viral antigens in infected cells using an immunoperoxidase method].
The use of immunoperoxidase method for detection of virus antigens in a fixed infected cell monolayer on microplates (cellular immunoperoxidase method, CIPM) for poliomyelitis and hepatitis A virus as well as for human and simian SA II rotaviruses is described, as well as the method of quantitation of rotaviruses by counting of virus multiplication foci. The CIPM combines the advantages of the methods based on the infectivity determinations and of immunochemical methods, and may be used both for diagnosis and for detection and quantitation of enteric viruses.
Amitina
N N
NN
Ginevskaia
V A
VA
Shirman
G A
GA
Baeva
L E
LE
Ivannikova
T I
TI
D000956:Antigens, Viral / Q000032:analysis*; D004770:Enterovirus / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
D000956:Antigens, Viral
Obnaruzhenie i kolichestvennoe opredelenie kishechnykh virusov s pomoshch'iu detektsii virusnykh antigenov v zarazhennykh kletkakh immunoperoksidaznym metodom.