title: Evidence for multiple molecular weight forms of somatostatin-like material in Escherichia coli.
abstract: Extracts of Escherichia coli grown in defined medium contain somatostatin-related material (1-10 pg/g wet weight of cells). Preconditioned medium had no immunoactive somatostatin whereas, conditioned medium had 110-150 pg/l. Following purification of the extracted material on Sep-pak C18, Bio-Gel P-6 and HPLC, multiple molecular weight forms of somatostatin- (SRIF-) related material were identified. The material in one peak reacted in both the N-terminal and C-terminal SRIF immunoassay and coeluted on HPLC with SRIF-28, whereas that in a second peak eluted near SRIF-14 and was reactive only in the C-terminal SRIF assay. The two peaks are thus similar to SRIF-28 and SRIF-14 of vertebrates. These findings add support to the suggestion that vertebrate-type peptide hormones and neuropeptides have early evolutionary origins.
authors.forename: D
authors.initials: D
authors.lastname: LeRoith
authors.forename: W
authors.initials: W
authors.lastname: Pickens
authors.forename: L K
authors.initials: LK
authors.lastname: Crosby
authors.forename: M
authors.initials: M
authors.lastname: Berelowitz
authors.forename: M
authors.initials: M
authors.lastname: Holtgrefe
authors.forename: J
authors.initials: J
authors.lastname: Shiloach
mesh_terms: D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis*; D008970:Molecular Weight; D013004:Somatostatin / Q000302:isolation & purification*; D053561:Somatostatin-28
chemical_list: D003470:Culture Media; D013004:Somatostatin; D053561:Somatostatin-28