Decreased B lymphocyte ecto-5'nucleotidase and increased adenosine deaminase in mononuclear cells from patients infected with human immunodeficiency virus.
The levels of purine enzyme activities were studied in 10 patients with acquired immunodeficiency syndrome (AIDS) or AIDS related complex (ARC) and in 6 healthy individuals with antibodies against human immunodeficiency virus (HIV). All AIDS/ARC patients studied had ecto-5'nucleotidase (ecto-5'NUC) activity in B lymphocytes below the normal range and 4 out of 6 clinically healthy HIV-positive likewise had reduced activity. Increased numbers of activated B lymphocytes were found both in the group of healthy HIV positive individuals and in AIDS/ARC patients. Further studies are needed to define whether the decrease in ecto-5'NUC activity on the B lymphocytes is a result of increased activation of the cells or of a B cell defect. No significant changes were found in ecto-5'NUC levels in T lymphocytes or mononuclear cells (MNC), neither in the group of AIDS/ARC patients nor in the healthy HIV-positive group. Both AIDS/ARC patients and healthy individuals with antibodies against HIV had increased levels of adenosine deaminase (ADA) activity in mononuclear cells, but only in the group of AIDS/ARC patients was the increase significant. No changes were found in purine nucleoside phosphorylase (PNP) activity in the two groups tested. From these investigations of purine enzyme levels and other markers of immune function in both sick and healthy HIV infected individuals we conclude that the observed changes in ecto-5'NUC and ADA activities in HIV infected patients are not a direct result of the HIV infection but develop early in the course of the disease.
Dalh Christensen
L
L
Department of Infectious Deseases, Rigshospitalet, Copenhagen, Denmark.
Svenson
M
M
Nygaard
P
P
Andersen
V
V
Faber
V
V
D015720:5'-Nucleotidase; D000386:AIDS-Related Complex / Q000201:enzymology* / Q000276:immunology; D000163:Acquired Immunodeficiency Syndrome / Q000201:enzymology* / Q000276:immunology; D000243:Adenosine Deaminase / Q000097:blood*; D001402:B-Lymphocytes / Q000201:enzymology*; D006679:HIV Seropositivity / Q000201:enzymology* / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification; D009700:Nucleoside Deaminases / Q000097:blood*; D009708:Nucleotidases / Q000097:blood*; D013601:T-Lymphocytes / Q000201:enzymology*
D007136:Immunoglobulins; D009708:Nucleotidases; D015720:5'-Nucleotidase; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase