Detection of endocrine cells by immunofluorescence method in the gastroenteropancreatic system of the adult eel, glass-eel, and leptocephalic larva (Anguilla anguilla L.).
Five antisera against insulin (Ins), glucagon (Glu), somatostatin (SRIF), met-enkephalin (met-enk), and serotonin (5-HT) were used for immunofluorescence detection of endocrine cells in pancreas and gastrointestinal tract (GIT) of the European eel (Anguilla anguilla L.) at three stages of development (leptocephalic larva, glass-eel, and adult eel). Comparable distribution of endocrine cells was observed for adults and glass-eels. In their pancreatic islets, positive immunoreactions were obtained only for Ins, SRIF, and Glu; this later was also present in the pancreatic ducts. 5-HT cells were present throughout the GIT. SRIF cells were situated mostly in the stomach and less in the intestine. Met-enk cells were abundant in the pyloric cecum, but less frequent in the intestinal mucosa. Glu cells were present only in the intestine. No insulin-immunoreactive cells could be detected in the GIT. The pancreatic islets of leptocephalic larvae exhibited a strong reaction for SRIF, a weak reaction for Glu, and none at all for Ins, met-Enk, or 5-HT. The GIT of these larvae contained numerous met-enk cells, mainly in the foregut. In the fore- and midgut, cells exhibited a weak fluorescence after treatment with Glu antiserum. No positive immunoreactive cells were observed with 5-HT, SRIF, or Ins antisera.
L'Hermite
A
A
Ferrand
R
R
Dubois
M P
MP
Andersen
A C
AC
D000811:Anguilla / Q000033:anatomy & histology / Q000254:growth & development*; D000818:Animals; D004064:Digestive System / Q000166:cytology* / Q000378:metabolism; D004702:Endocrine Glands / Q000166:cytology* / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005934:Glucagon / Q000378:metabolism; D006651:Histocytochemistry; D007328:Insulin / Q000378:metabolism; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism
D007328:Insulin; D012701:Serotonin; D013004:Somatostatin; D004744:Enkephalin, Methionine; D005934:Glucagon