title: Receptors of prostaglandin E1 in the plasma membrane of normal liver. Effect of membrane fluidity on PGE1 binding.
abstract: Prostaglandin E1 binding to isolated liver plasma membrane as a function of PGE1 concentration showed saturability of the binding sites at PGE1 concentration of 2.5 X 10(-4) M. Scatchard analysis revealed heterogeneous population of binding sites with a binding capacity of 470 and 990 nmol/mg protein for the higher and lower affinity binding sites respectively. PGE1 binding to liver plasma membrane was progressively and significantly decreased (P less than 0.01) as the incubation temperature was reduced to 22 degrees and 4 degrees C. Procaine at 1 X 10(-3) M concentration showed a significant decrease (P less than 0.01) in the binding capacity of the liver plasma membrane. Colchicine plus cytochalasin-B inhibited PGE1 binding significantly (P less than 0.01) but their inhibition is not equivalent to that of procaine.
authors.forename: C F
authors.initials: CF
authors.lastname: Nassar
authors.forename: M G
authors.initials: MG
authors.lastname: Nasser
authors.forename: Z M
authors.initials: ZM
authors.lastname: Habbal
mesh_terms: D000527:Alprostadil / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology; D003080:Cold Temperature; D003571:Cytochalasin B / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D011343:Procaine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011982:Receptors, Prostaglandin / Q000187:drug effects / Q000378:metabolism*; D018078:Receptors, Prostaglandin E
chemical_list: D003598:Cytoskeletal Proteins; D011956:Receptors, Cell Surface; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D003571:Cytochalasin B; D011343:Procaine; D000527:Alprostadil; D003078:Colchicine