Acetylcholine receptors in the human retina.
Evidence for a population of acetylcholine (ACh) receptors in the human retina is presented. The authors have used the irreversible ligand 3H-propylbenzilylcholine mustard (3H-PrBCM) to label muscarinic receptors. 3H- or 125I-alpha-bungarotoxin (alpha-BTx) was used to label putative nicotinic receptors. Muscarinic receptors are apparently present in the inner plexiform layer of the retina. Autoradiographic grain densities are reduced in the presence of saturating concentrations of atropine, quinuclidinyl benzilate or scopolamine; this indicates that 3H-PrBCM binding is specific for a population of muscarinic receptors in the human retina. Binding sites for radiolabeled alpha-BTx are found predominantly in the inner plexiform layer of the retina. Grain densities are reduced in the presence of d-tubocurarine, indicating that alpha-BTx may bind to a pharmacologically relevant nicotinic ACh receptor. The work reported here is consistent with earlier data on cholinergic neurons in the retina of other mammalian species, as well as with previous work on the psychophysical effects of cholinergic agonists on human vision. This study provides evidence for cholinergic neurotransmission in the human retina.
Hutchins
J B
JB
Hollyfield
J G
JG
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002038:Bungarotoxins; D006801:Humans; D018377:Neurotransmitter Agents / Q000032:analysis*; D011438:Propylbenzilylcholine Mustard; D011817:Rabbits; D011950:Receptors, Cholinergic / Q000032:analysis* / Q000502:physiology; D011976:Receptors, Muscarinic / Q000032:analysis; D011978:Receptors, Nicotinic / Q000032:analysis; D012160:Retina / Q000032:analysis* / Q000502:physiology; D014316:Tritium; D014785:Vision, Ocular
D002038:Bungarotoxins; D018377:Neurotransmitter Agents; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D014316:Tritium; D011438:Propylbenzilylcholine Mustard; D000110:Acetylcholinesterase