[Dipeptidyl peptidase IV. Purification for use in peptide sequencing].
Isolation and purification of dipeptidyl peptidase IV from pig kidney are described. The specific activity of the enzyme is 24.9 U/mg protein. Chromatography on Gly-Pro-AH-Sepharose is the most important procedure for the separation from other enzyme activities. The enzyme preparation is free of aminopeptidase, dipeptidase and prolyl endopeptidase activity; it can be used for peptide-sequence analysis.
Gonschor
H
H
Schäfer
W
W
D000595:Amino Acid Sequence; D000645:Ammonium Sulfate; D000818:Animals; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D007525:Isoelectric Focusing; D007668:Kidney / Q000201:enzymology; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis; D013552:Swine
D011506:Proteins; D010450:Endopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4; D000645:Ammonium Sulfate
Dipeptidyl-Peptidase IV. Reinigung für die Verwendung zur Peptidsequenzierung.