Immunoassay of the neuronal and neuroendocrine marker PGP 9.5 in human tissues.
An inhibitory, coated-well immunoassay for the neurone-specific protein PGP 9.5 has been devised and used to measure the concentrations of the protein in human tissues. Concentrations of PGP 9.5 between 40 ng/ml and 10 micrograms/ml could be measured using this assay. In brain PGP 9.5 was present at 100.58 +/- 16.18 micrograms/mg protein. Of the other organs examined only kidney and testis showed significant concentrations of PGP 9.5 (3.97 +/- 0.87 microgram/mg protein and 3.25 +/- 0.36 microgram/mg protein, respectively). All other organs contained less than 2% of the brain level. The tissue levels determined by coated-well immunoassay confirmed the tissue specificity of PGP 9.5 originally determined by high-resolution two-dimensional gel electrophoresis.
Bradbury
J M
JM
Thompson
R J
RJ
D001078:APUD Cells / Q000032:analysis; D001923:Brain Chemistry; D006801:Humans; D007118:Immunoassay; D007668:Kidney / Q000032:analysis; D008297:Male; D008832:Microchemistry; D009419:Nerve Tissue Proteins / Q000032:analysis*; D013737:Testis / Q000032:analysis; D014018:Tissue Distribution; D043222:Ubiquitin Thiolesterase
D009419:Nerve Tissue Proteins; D043222:Ubiquitin Thiolesterase