High affinity receptors for atrial natriuretic factor in PC12 cells.
Specific receptors for atrial natriuretic factor (ANF) are characterized on PC12 cells (rat pheochromocytoma cell line). Radioiodinated synthetic ANF (Rat, 8-33) bound to a single class of high affinity binding sites with the Kd value of 6.7 X 10(-10) M. The Bmax value was 29 fmol/10(5) cells and receptor density was calculated as 194,000 +/- 20,000/cell. Photoaffinity labelling of ANF receptor specifically labelled two protein bands with apparent m.wt of 70,000 and 130,000. When the cells were incubated with the labelled ligand at 37 degrees C the ligand was internalized. The rate of internalization increased in the presence of increased ligand concentration. ANF receptors on PC12 cells are reported for the first time which would provide a unique model for study of ANF-receptor interaction.
Rathinavelu
A
A
Department of Pharmacology and Toxicology, School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, IN 47907.
Isom
G E
GE
D000310:Adrenal Gland Neoplasms; D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D010673:Pheochromocytoma; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013388:Succinimides / Q000378:metabolism
D000345:Affinity Labels; D011956:Receptors, Cell Surface; D013388:Succinimides; D009320:Atrial Natriuretic Factor; C035760:bis(sulfosuccinimidyl)suberate; D017461:Receptors, Atrial Natriuretic Factor