Effects of phospholipases on Cl-ATPase in the rat brain.
We investigated the effects of phospholipases on the activity of microsomal Cl-ATPase in the rat brain, in reference to those on the activities of Na,K-ATPase and anion-insensitive Mg-ATPase. In the presence of phospholipase A2 or phospholipase C, which almost completely hydrolyzed microsomal phosphoglycerides, the activities of Cl-ATPase and Na,K-ATPase were decreased to 8-50% of the control, but anion-insensitive Mg-ATPase activity was not altered. On the other hand, with sphingomyelinase treatment, only anion-insensitive Mg-ATPase was slightly inactivated. On the addition of phospholipids (phosphatidylserine (PS), phosphatidylinositol (PI) and microsomal phospholipid mixture), Cl-ATPase activity slightly recovered only with PI, while Na,K-ATPase activity partially recovered with either phospholipid. These data suggest that Cl-ATPase requires intact membrane lipid conformation and especially PI for its maximal activity.
Kunugi
Y
Y
Department of Biology, Kyoto Pharmaceutical University, Japan.
Hashimoto
Y
Y
Tamura
A
A
Fujii
T
T
Inagaki
C
C
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D027321:Anion Transport Proteins; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D010740:Phospholipases / Q000494:pharmacology*; D010741:Phospholipases A / Q000494:pharmacology; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013108:Sphingomyelin Phosphodiesterase / Q000494:pharmacology; D010738:Type C Phospholipases / Q000494:pharmacology
D027321:Anion Transport Proteins; D010743:Phospholipids; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases; D013108:Sphingomyelin Phosphodiesterase; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; C031652:anion-sensitive ATPases; D000254:Sodium-Potassium-Exchanging ATPase