Complete sequence of neutralization protein VP2 of the recent US isolate bluetongue virus serotype 2: its relationship with VP2 species of other US serotypes.
In order to determine the relationship between the most recently isolated bluetongue virus serotype (BTV-2), and other US serotypes, the complete nucleotide sequence was determined from cDNA clones representing the L2 dsRNA of BTV-2, the gene that codes for the outer capsid neutralization antigen (VP2). The predicted amino acid sequence of the protein was compared with the VP2 sequences of the US serotypes BTV-10, BTV-11, BTV-13 and BTV-17. The VP2 protein of BTV-2 was found to exhibit 47% homology with the VP2 species of BTV-13, but only 40-41% homology with the VP2 species of earlier US isolates, BTV-10-, and -17. However, Diagon comparisons and hydropathic plots of all five VP2 species indicated that all of them are structurally very similar.
Yamaguchi
S
S
School of Public Health, University of Alabama, Birmingham.
Fukusho
A
A
Roy
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012087:Reoviridae / Q000235:genetics*; D012703:Serotyping; D012756:Sheep; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins
D014764:Viral Proteins; D015678:Viral Structural Proteins; D004247:DNA