In vivo mammalian metabolism of methylamine and methylurea and their metabolic interrelationship.
The metabolism of methylamine has been investigated in the rabbit and rat, and evidence has been presented to show that the metabolism of the compound leads to the formation and excretion of methylurea, a metabolite suspected, but never conclusively shown, to exist by previous investigators. Urinary methylurea, after the administration of methyl-14C-amine, was shown to have 14C activity at both the carbonyl and methyl portions (groups) of the molecule. 14C activity in the carbonyl group of methylurea was derived from the same metabolic CO2 pool that is used in the formation of urinary urea and respiratory carbon dioxide. Administration of methyl-14C-amine led to the formation of respiratory 14CO2, as noted by various investigators, and it was shown that during a 24-hr period the ratio of specific activity (urea/respiratory CO2) approached 1, in agreement with previous data reported by Mackenzie and du Vigneaud [J. Biol. Chem. 172, 353-354 (1948)]. In order to quantitatively investigate some aspects of metabolism of methylurea, a procedure was developed for the convenient synthesis of N-methyl-14C-carbonyl-urea. In a series of experiments, the urinary urea of the animals injected with N-methyl-14C-carbonyl-urea and N-methyl-14C-urea was examined for radioactivity. Only limited amounts of 14C activity were found in the carbonyl group of urea following administration of N-methyl-14C-urea. However, after the administration of N-methyl-14C-carbonyl-urea, urinary urea contained a 200-fold excess of 14C activity.(ABSTRACT TRUNCATED AT 250 WORDS)
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D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D005260:Female; D006868:Hydrolysis; D008744:Methylamines / Q000378:metabolism*; D008782:Methylurea Compounds / Q000378:metabolism*; D010084:Oxidation-Reduction; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014508:Urea / Q000378:metabolism
D008744:Methylamines; D008782:Methylurea Compounds; D002245:Carbon Dioxide; D014508:Urea