Dose-dependent effects of butylated hydroxyanisole, butylated hydroxytoluene and ethoxyquin in induction of foci of rat liver cells containing the placental form of glutathione S-transferase.
The dose-dependence effects of 3 antioxidants, butylated hydroxyanisole (BHA: 2.0%, 1.0% and 0.5%), butylated hydroxytoluene (BHT: 1.0%, 0.5% and 0.25%) and ethoxyquin (EQ: 0.5%, 0.25% and 0.125%) combined with partial hepatectomy on the development of preneoplastic lesions in the liver of diethylnitrosamine (DEN, 200 mg/kg body wt)-treated rats were investigated. Feeding of the antioxidants commenced 2 weeks after the single dose of DEN used to initiate the lesions. gamma-Glutamyl transpeptidase (gamma-GT) and the placental form of glutathione S-transferase (GST-P) were used for quantitation of altered focal populations. Results with both markers demonstrated a dose-dependent decrease of foci in BHA-treated rats relative to those in control rats. Morphometric analysis of gamma-GT-positive lesions also revealed decrease in both the number and area of foci in BHT- and EQ-treated groups. The discrepancy between results of quantitation of gamma-GT- and GST-P-positive foci was attributable to the induction of a background, periportal zone staining for gamma-GT, which made differentiation of smaller foci difficult. Comparison of results with the 2 markers suggested that GST-P is the more accurate marker for quantitative studies on enzyme altered foci in rat liver.
Thamavit
W
W
Tatematsu
M
M
Ogiso
T
T
Mera
Y
Y
Tsuda
H
H
Ito
N
N
D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D004052:Diethylnitrosamine; D004305:Dose-Response Relationship, Drug; D005015:Ethoxyquin / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000032:analysis*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010920:Placenta / Q000201:enzymology*; D011230:Precancerous Conditions / Q000139:chemically induced; D011804:Quinolines / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D000840:Anisoles; D011804:Quinolines; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D004052:Diethylnitrosamine; D005015:Ethoxyquin; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase