title: CYP1 (HAP1) regulator of oxygen-dependent gene expression in yeast. I. Overall organization of the protein sequence displays several novel structural domains.
abstract: In the yeast Saccharomyces cerevisiae the CYP1 gene that modulates the expression of iso1-(CYC1) and iso2-cytochrome c (CYP3) structural genes gives rise to two classes of mutated alleles; one class, represented by CYP1-18, has opposite effects on CYC1 and CYP3, it reduces the expression of CYC1 while it stimulates that of CYP3. The other class, represented by cyp1-23 or the related allele hap1-1, reduces the expression of both CYC1 and CYP3 genes. Genetic data suggested that the CYP1 product is a positive regulator of the cytochrome c genes. The CYP1-18 allele has been cloned. We show here that the iso2 overproducer function of CYP1-18 is included in a 5300 base XhoI-PstI fragment. The sequence of this fragment reveals a unique, long, uninterrupted open reading frame of 4449 nucleotides able to encode a protein of 1483 amino acid residues. The predicted product of this open reading frame contains several interesting features. The N-terminal part of the protein resembles a nucleic acid-binding domain, in which two domains can be distinguished. The first is similar to a "finger" DNA binding motif, as found in TFIIIA and other regulatory proteins. The second consists of seven tandemly repeated sequences with a KCPVDH motif. Because of its structure, it is tempting to speculate that this region may act as a "redox sensor" folded around a metal atom or heme and involved in recognition of respiratory effectors. These two domains are separated by an "opa" sequence of 13 Gln residues. Implication of these domains for the function of CYP1-18 is discussed.
authors.affiliation: Centre de Génétique Moléculaire du C.N.R.S., Laboratoire propre associé à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France.
authors.forename: F
authors.initials: F
authors.lastname: Creusot
authors.forename: J
authors.initials: J
authors.lastname: Verdière
authors.forename: M
authors.initials: M
authors.lastname: Gaisne
authors.forename: P P
authors.initials: PP
authors.lastname: Slonimski
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D008969:Molecular Sequence Data; D010100:Oxygen; D012331:RNA, Fungal / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D003574:Cytochrome c Group; D005656:Fungal Proteins; D012331:RNA, Fungal; D010100:Oxygen