DNA-binding activity associated with the v-myb oncogene product is not sufficient for transformation.
The product of the v-myb oncogene of avian myeloblastosis virus is a nuclear protein with an associated DNA-binding activity. We demonstrated that the highly conserved amino-terminal domain of p48v-myb is required for its associated DNA-binding activity. This activity is not required for the nuclear localization of p48v-myb. Furthermore, the associated DNA-binding activity and nuclear localization of p48v-myb together are not sufficient for transformation.
Ibanez
C E
CE
Department of Pathology, University of California, San Diego, La Jolla 92093-0612.
Garcia
A
A
Stober-Grässer
U
U
Lipsick
J S
JS
D001345:Autoradiography; D001354:Avian Leukosis Virus / Q000472:pathogenicity*; D009189:Avian Myeloblastosis Virus / Q000235:genetics / Q000472:pathogenicity*; D001665:Binding Sites; D002472:Cell Transformation, Viral; D004268:DNA-Binding Proteins / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D009154:Mutation; D020626:Oncogene Proteins v-myb; D011233:Precipitin Tests; D011519:Proto-Oncogenes; D012191:Retroviridae Proteins / Q000235:genetics / Q000502:physiology*; D014758:Viral Core Proteins / Q000235:genetics / Q000502:physiology*
D004268:DNA-Binding Proteins; D020626:Oncogene Proteins v-myb; D012191:Retroviridae Proteins; D014758:Viral Core Proteins