title: Inhibitory effect of neurohypophysial peptides on cyclic AMP accumulation by isolated hepatocytes of the trout.
abstract: Cyclic AMP levels were measured in freshly isolated hepatocytes of the rainbow trout. Compared with basal values, the average levels were increased up to 60 times in a dose-dependent manner either by mammalian glucagon (concentration range 1 nmol-1 mumol/l; dose giving half maximum response (EC50) 0.18 mumol/l) or by forskolin (concentration range 0.1-100 mumol/l; EC50 about 10 mumol/l). These stimulatory effects were partially inhibited by fish or mammalian neurohypophysial hormones used at relatively high concentrations (1-5 mumol/l). It is suggested that these results are evidence for the presence of V1-type receptors in fish hepatocytes. Together with previous results obtained with gills on the hormonal inhibition of adenylate cyclase activity, they suggest that teleost fish may possess only V1-type receptors (or two V1-related types), while the V2 receptors have evolved (or have become functional) in higher vertebrates.
authors.affiliation: Laboratoire de Physiologie Cellulaire et Comparée and UA CNRS 651, Faculté des Sciences, Nice, France.
authors.forename: B
authors.initials: B
authors.lastname: Lahlou
authors.forename: B
authors.initials: B
authors.lastname: Fossat
authors.forename: J
authors.initials: J
authors.lastname: Porthé-Nibelle
authors.forename: L
authors.initials: L
authors.lastname: Bianchini
authors.forename: M
authors.initials: M
authors.lastname: Guibbolini
mesh_terms: D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects*; D010909:Pituitary Hormones, Posterior / Q000494:pharmacology*; D012487:Salmonidae / Q000502:physiology*; D013997:Time Factors; D014337:Trout / Q000502:physiology*
chemical_list: D010909:Pituitary Hormones, Posterior; D005576:Colforsin; D005934:Glucagon; D000242:Cyclic AMP