Effect of potent antiglucocorticoids on dexamethasone induced enzymes in cultured hepatoma and rat liver cells.
The antagonism by 3 synthetic steroids of the induction of tyrosine aminotransferase and tryptophan dioxygenase by dexamethasone were compared in HTC and FAZA hepatoma cells and in isolated liver cells. It was observed: (i) the sensitivity of liver cells to glucocorticoid agonists and antagonists changed in relation to time of culture; (ii) the extent of the inhibitory effect on enzyme induction depends on the nature of the antagonist; (iii) hepatoma cells, especially HTC cells, appeared more sensitive to glucocorticoid antagonism than liver cells.
Santana
M A
MA
Chasserot-Bolaz
S
S
Beck
B
B
Pogson
C I
CI
D000818:Animals; D002478:Cells, Cultured; D003907:Dexamethasone / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D004963:Estrenes / Q000494:pharmacology; D005938:Glucocorticoids / Q000037:antagonists & inhibitors*; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008297:Male; D015735:Mifepristone; D009649:Norpregnadienes / Q000494:pharmacology / Q000633:toxicity*; D011397:Promegestone / Q000494:pharmacology / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D014366:Tryptophan Oxygenase / Q000096:biosynthesis; D014444:Tyrosine Transaminase / Q000096:biosynthesis
D004963:Estrenes; D005938:Glucocorticoids; D009649:Norpregnadienes; D015735:Mifepristone; C046120:RU 25055; D003907:Dexamethasone; D011397:Promegestone; D014366:Tryptophan Oxygenase; D014444:Tyrosine Transaminase