title: Polymorphism and immunochemical cross-reactivity of acetylcholinesterases from the brains of human, dog, hog, bovine and horse.
abstract: In the caudate nucleus of the species tested about 20% of the acetylcholinesterase was salt soluble and sedimented in sucrose density gradient centrifugation as monomeric 5 S and tetrameric 10 S enzyme. About 80% was solubilized by micellar concentrations of Triton X-100 and sedimented as a tetrameric 10 S species in the presence of detergent but formed aggregates in the absence thereof. All the enzyme displayed poor cross-reactivity with a precipitating assay (Ouchterlony) but in a solid phase non-precipitating assay the cross-reactivity could be quantified and ranged from 96 to less than 1% depending on the species.
authors.forename: K
authors.initials: K
authors.lastname: Sorensen
authors.forename: K
authors.initials: K
authors.lastname: Gennari
authors.forename: U
authors.initials: U
authors.lastname: Brodbeck
authors.forename: P
authors.initials: P
authors.lastname: Landauer
authors.forename: M
authors.initials: M
authors.lastname: Liefländer
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics* / Q000276:immunology; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002499:Centrifugation, Density Gradient; D003429:Cross Reactions; D004285:Dogs; D004912:Erythrocytes / Q000201:enzymology; D006736:Horses; D006801:Humans; D005779:Immunodiffusion; D013552:Swine
chemical_list: D000110:Acetylcholinesterase