Effect of Ni2+ on Ca2+-stimulated adenosine triphosphatase in the microsomal fraction of the rat parotid gland in vitro.
Ni2+ inhibited Ca2+-stimulated adenosine triphosphatase activity in the microsomal fraction of the rat parotid gland in vitro. The Ni2+ concentration required for 50% inhibition was 0.45 mM. Inhibition mechanisms of Ni2+ for Ca2+ and ATP were of the competitive type and the noncompetitive type, respectively. The Ki values of Ni2+ for Ca2+ and ATP were 0.52 and 0.59 mM, respectively. The inhibitory effect of Ni2+ was reversible.
Ikeda
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D000818:Animals; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D009532:Nickel / Q000494:pharmacology*; D010306:Parotid Gland / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
C022838:nickel chloride; D009532:Nickel; D000252:Calcium-Transporting ATPases