Identification of the DNA sequence from the E. coli terminus region that halts replication forks.
The terminus region of the E. coli chromosome contains two loci, T1 and T2, that inhibit the progress of replication forks and require the trans-acting factor tus. We have identified a 23 bp terminator signal at T1 and T2 that is within 100 bp of the sites of replication arrest. When an oligodeoxyribonucleotide containing the terminator signal was inserted into a plasmid, replication was halted only in a tus+ strain and when the terminator signal was oriented properly. We also found this terminator sequence in the terminus region of the plasmid R6K and in the origin region of RepFIIA class plasmids. In addition, we found striking similarities between the E. coli terminator signal and the terminator sequence of B. subtilis.
Hill
T M
TM
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.
Pelletier
A J
AJ
Tecklenburg
M L
ML
Kuempel
P L
PL
D001483:Base Sequence; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D015719:Single-Strand Specific DNA and RNA Endonucleases
D004269:DNA, Bacterial; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases