Bacillus subtilis contains multiple forms of somatostatin-like material.
Extracts of B. subtilis contain somatostatin-like immunoactivity (1-20 pg per g wet weight cells). Two major forms were detected, one with reactivity in both N- and C-terminal immunoassays similar to somatostatin-28 and a second form reactive only in the C-terminal specific immunoassay similar to somatostatin-14. Both forms were active in a bioassay and the bioactivity was neutralized in the presence of antibody to the central, biologically active part of somatostatin-14. Preconditioned medium contained no detectable somatostatin whereas conditioned medium had 80-380 pg per liter.
LeRoith
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D
Pickens
W
W
Vinik
A I
AI
Shiloach
J
J
D000818:Animals; D001412:Bacillus subtilis / Q000032:analysis* / Q000378:metabolism*; D001681:Biological Assay; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000378:metabolism; D013004:Somatostatin / Q000032:analysis* / Q000494:pharmacology; D053561:Somatostatin-28
D006878:Hydroxides; D017665:Hydroxyl Radical; D013004:Somatostatin; D053561:Somatostatin-28