dpy-13: a nematode collagen gene that affects body shape.
Mutations in the Caenorhabditis elegans dpy-13 (dumpy) gene result in a short, chunky body shape. This gene was tagged by insertion of the Tc1 transposon, and the wild-type gene was cloned by chromosomal walking 11 kb from ama-1, a cloned gene encoding the large subunit of RNA polymerase II. Three transposon insertion sites in dpy-13 are located near the 5' end of a 1.2 kb transcribed region. The EMS-induced reference allele, dpy-13(e184), carries a small deletion near the middle of this gene. The DNA sequence reveals that dpy-13 is a member of the collagen multi-gene family, and it could encode a polypeptide of 302 amino acids. A 146 base pair sequence, encoding amino acids 56-103, is unique in the C. elegans genome, and it hybridizes to a 1 kb mRNA of moderate abundance.
von Mende
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Division of Biological Sciences, University of Missouri, Columbia 65211.
Bird
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Albert
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Riddle
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D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002107:Caenorhabditis / Q000235:genetics* / Q000254:growth & development; D002872:Chromosome Deletion; D003094:Collagen / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D009154:Mutation; D012319:RNA Polymerase II / Q000235:genetics
D004251:DNA Transposable Elements; D003094:Collagen; D004247:DNA; D012319:RNA Polymerase II