Amphotericin B inhibits the serum-induced expression of tissue transglutaminase in murine peritoneal macrophages.
Culture of mouse resident peritoneal macrophages (PM) in serum-containing medium causes a rapid and marked induction of the enzyme tissue transglutaminase (tissue TGase). Coculture of PM with amphotericin B (AmpB) inhibited the serum-induced expression and accumulation of tissue TGase. The AmpB-mediated inhibition of tissue TGase was specific and was due to inhibition of enzyme synthesis. The serum-dependent induction of tissue TGase was inhibited in a dose-dependent fashion, and a complete inhibition was observed at 1.5 microgram/ml dose of AmpB. The inhibition was reversible; however, the time of recovery depended on the dose and time of exposure of the cells to AmpB. The present studies suggest that AmpB-mediated inhibition of tissue TGase is due to inhibition of the uptake of serum retinoids by PM.
Mehta
K
K
Claringbold
P
P
Lopez-Berestein
G
G
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D003470:Culture Media; D007700:Kinetics; D008264:Macrophages / Q000201:enzymology* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity; D011956:Receptors, Cell Surface / Q000187:drug effects; D012176:Retinoids / Q000494:pharmacology; D012177:Retinol-Binding Proteins / Q000378:metabolism; D011503:Transglutaminases / Q000037:antagonists & inhibitors*
D003470:Culture Media; D011956:Receptors, Cell Surface; D012176:Retinoids; D012177:Retinol-Binding Proteins; C027928:retinol binding protein receptor; D000666:Amphotericin B; D011503:Transglutaminases