Dopaminergic inhibition of alpha-melanocyte-stimulating hormone release from superfused slices of the rat hypothalamus.
The dopamine agonist apomorphine (10(-6) M) significantly reduced the 50 mM K+-stimulated, Ca2+-dependent release of immunoreactive alpha-melanocyte-stimulating hormone (alpha-MSH) from superfused slices of rat hypothalamus but did not alter baseline peptide release. Flupenthixol (10(-6) M), a dopamine receptor antagonist, significantly increased both basal and 50 mM K+-stimulated alpha-MSH release, possibly via blockade of inhibition caused by endogenous dopamine release. The latter increases were Ca2+-dependent and were inhibited by 10(-6) M apomorphine.
Tiligada
E
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Department of Pharmacology and Therapeutics, University of Wales College of Medicine, Cardiff, U.K.
Wilson
J F
JF
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D004298:Dopamine / Q000502:physiology*; D005475:Flupenthixol / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013892:Thioxanthenes / Q000494:pharmacology*; D000521:alpha-MSH / Q000378:metabolism*
D013892:Thioxanthenes; D000521:alpha-MSH; D005475:Flupenthixol; D001058:Apomorphine; D011188:Potassium; D004298:Dopamine