title: Plant glutamine synthetase complements a glnA mutation in Escherichia coli.
abstract: A glutamine synthetase gene from alfalfa (Medicago sativa) has been expressed in Escherichia coli after fusion of bacterial transcription and translation signals to a complete alfalfa glutamine synthetase coding sequence. Synthesis of the alfalfa glutamine synthetase enzyme in Escherichia coli was demonstrated by functional genetic complementation of a glutamine synthetase-deficient mutant and by immunoblotting analysis. These results should facilitate protein engineering and structure-function analysis of the plant enzyme.
authors.forename: S
authors.initials: S
authors.lastname: DasSarma
authors.forename: E
authors.initials: E
authors.lastname: Tischer
authors.forename: H M
authors.initials: HM
authors.lastname: Goodman
mesh_terms: D001483:Base Sequence; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005816:Genetic Complementation Test; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D000455:Medicago sativa / Q000235:genetics*; D008970:Molecular Weight; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D004274:DNA, Recombinant; D005974:Glutamate-Ammonia Ligase