title: [Regulation of adenosine triphosphatase activity in mitochondria, chloroplasts and erythrocytes by anions, adenosine diphosphate and alcohols].
abstract: The effects of sulfite, bicarbonate, thiocyanate, methanol, ethanol, glycerol, dimethy sulfoxide and ADP on the ATPase activity of the coupling factor from liver mitochondria (F1) and pea chloroplasts (CF1) and of the anion-sensitive ATPase from rat erythrocytes were investigated. Under steady-state conditions of ATP hydrolysis catalyzed by F1, CF1, and erythrocyte ATPase, three Km values for each of the enzymes, three activation constants for sulfite and three inhibition constants for thiocyanate were determined. The efficiency and direction of the effects of anions, alcohols and ADP strongly depend on temperature and substrate (Mg-ATP) concentration. The mechanisms of modification by anions and alcohols of the ATPase activities are discussed.
authors.forename: A T
authors.initials: AT
authors.lastname: Ivashchenko
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: K R
authors.initials: KR
authors.lastname: Uteulin
authors.forename: S V
authors.initials: SV
authors.lastname: Ponomarenko
authors.forename: I A
authors.initials: IA
authors.lastname: Bushneva
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000438:Alcohols / Q000494:pharmacology; D000818:Animals; D000838:Anions / Q000494:pharmacology; D002736:Chloroplasts / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000201:enzymology*; D007887:Fabaceae; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology*; D010946:Plants, Medicinal; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats
chemical_list: D000438:Alcohols; D000838:Anions; D000244:Adenosine Diphosphate; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases
vernacular_title: Reguliatsiia anionami, adenozindifosfatom i spirtami aktivnosti adenozintrifosfataz mitokhondriĭ, khloroplastov i éritrotsitov.