[Identification of alpha-2 adrenergic receptors in rabbit adipocytes].
Adrenaline, an alpha and beta adrenergic agonist don't modify theophylline induced lipolysis in perirenal and epididymal adipose tissue of the rabbit. Clonidine an alpha 2 adrenergic agonist inhibits theophylline stimulated lipolysis in the two tissue indicating the existence of alpha 2 adrenergic responsiveness. Direct identification of these receptors by radioligand binding studies shows that (H3) yohimbine an alpha 2 adrenergic antagonist binds to rabbit's fat cell membranes with high affinity: KD = 1.7 +/- 0.1 nmoles. The maximal number of binding sites at saturation is low 16 +/- 29 fmoles/mg protein.
Nasraoui
A
A
Institut National de Nutrition de Tunis, Paris, France.
Rozen
R
R
Apfelbaum
M
M
D000273:Adipose Tissue / Q000032:analysis* / Q000166:cytology / Q000187:drug effects; D000818:Animals; D003000:Clonidine / Q000494:pharmacology*; D004837:Epinephrine / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D011942:Receptors, Adrenergic, alpha / Q000032:analysis* / Q000187:drug effects; D013806:Theophylline / Q000494:pharmacology*; D015016:Yohimbine / Q000494:pharmacology*
D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine; D013806:Theophylline; D003000:Clonidine; D004837:Epinephrine
Identification des récepteurs alpha 2 adrénergiques sur les adipocytes de lapin.