Inhibition of the proteolysis of rat erythrocyte membrane proteins by a synthetic inhibitor of calpain.
A synthetic inhibitor of calpain protects rat erythrocyte membrane-associated cytoskeletal proteins from proteolytic degradation (IC50 = 1 microM) which occurs when the cells are rendered permeable to Ca++. Leupeptin, a naturally occurring inhibitor of the enzyme, does not afford any protection at concentrations up to 100 microM.
Mehdi
S
S
Merrell Dow Research Institute, Cincinnati, OH 45215.
Angelastro
M R
MR
Wiseman
J S
JS
Bey
P
P
D000818:Animals; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood; D002154:Calpain / Q000037:antagonists & inhibitors*; D002463:Cell Membrane Permeability; D003598:Cytoskeletal Proteins / Q000097:blood*; D004151:Dipeptides / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000032:analysis* / Q000187:drug effects; D006868:Hydrolysis; D007976:Leupeptins / Q000494:pharmacology; D008565:Membrane Proteins / Q000097:blood*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000097:blood*; D011480:Protease Inhibitors; D051381:Rats; D013049:Spectrin / Q000378:metabolism
D003598:Cytoskeletal Proteins; D004151:Dipeptides; D007976:Leupeptins; D008565:Membrane Proteins; D011480:Protease Inhibitors; D013049:Spectrin; D000001:Calcimycin; D010447:Peptide Hydrolases; D002154:Calpain; C032854:leupeptin; D002118:Calcium; C058076:calpain inhibitor III