title: Characterization of specific binding of atrial natriuretic peptide (ANP) to rat PC12 phaeochromocytoma cells.
abstract: Specific binding sites for atrial natriuretic peptide have been identified in membrane of the phaeochromocytoma cell line PC12. Scatchard analysis of binding studies revealed a Kd of 794 pM and a density (Bmax) of 254 fmol/mg protein. Hormones unrelated to ANP such as angiotensin II, bradykinin and arginine-8-vasopressin did not complete for the binding sites. Of the ANP-related peptides which competed for the binding sites, the following order of affinity was established; rANP (8-33) greater than rANP (28 amino acid) greater than rat atrial peptide fragment (13-28) greater than a-hANP (28 amino acid) greater than atrial peptide fragment (1-11) greater than atriopeptin I.
authors.affiliation: MRC Blood Pressure Unit, Western Infirmary, Glasgow, Scotland, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Boumezrag
authors.forename: F
authors.initials: F
authors.lastname: Lyall
authors.forename: J A
authors.initials: JA
authors.lastname: Dow
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001667:Binding, Competitive; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D010673:Pheochromocytoma / Q000378:metabolism; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor