title: The effects of hypoxia on catecholamine dynamics in the rat carotid body.
abstract: The catecholamine content of the rat carotid body was assayed using high performance liquid chromatography with electrochemical detection. The concentration of dopamine (DA) was found to predominate over that of norepinephrine (NE) by a small margin (31 pmol/carotid body pair DA; 23 pmol/carotid body pair NE). The turnover rates of carotid body DA and NE were determined from the time-dependent decline in their concentrations following the blockade of synthesis with alpha-methyl-p-tyrosine. Values were obtained (DA t 1/2 = 1.9 h; NE t 1/2 = 2.3 h) which suggested a rapid turnover of carotid body catecholamines. Exposure of rats to conditions of severe hypoxia (5% O2-95% N2) failed to significantly alter either the content or turnover of carotid body catecholamines. By contrast, the concentration of carotid body DOPAC, a reflection of DA utilization, was significantly elevated following hypoxic conditions. Further, in vivo tyrosine hydroxylase activity was assessed by measuring the accumulation of carotid body DOPA after inhibiting L-aromatic amino acid decarboxylase with NSD-1015. Basal tyrosine hydroxylase activity (approximately 14-16 pmol/carotid body pair/h) also was significantly increased by acute hypoxic exposure. These results, in part, suggest that rat carotid body DA may act as a neurotransmitter whose synthesis and release are coupled to stimulus demand.
authors.forename: J J
authors.initials: JJ
authors.lastname: Brokaw
authors.forename: J T
authors.initials: JT
authors.lastname: Hansen
authors.forename: D S
authors.initials: DS
authors.lastname: Christie
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D002344:Carotid Body / Q000032:analysis / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism*; D004295:Dihydroxyphenylalanine / Q000032:analysis; D004298:Dopamine / Q000032:analysis; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D002395:Catecholamines; D015102:3,4-Dihydroxyphenylacetic Acid; D004295:Dihydroxyphenylalanine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine; D009638:Norepinephrine