Synthesis of sequences 1-16 and 63-95 of Cerebratulus lacteus toxin AIII. Hemolytic activity in a toxin fragment.
Sequences 1-16 and 63-95 of C. lacteus toxin AIII were synthesized on benzhydrylamine and PAM resins respectively; the crude peptide products released by HF contained 90 and 33% of the target peptides. Fragment 63-95 was nearly a full agonist, producing greater than 90% of the hemolytic activity of the intact toxin molecule at 10(3)-fold higher concentrations. Fragment 1-16 had no activity.
Balasubramaniam
A
A
Murphy
R F
RF
Blumenthal
K M
KM
D000595:Amino Acid Sequence; D000818:Animals; D003064:Cnidarian Venoms / Q000138:chemical synthesis* / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007202:Indicators and Reagents; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000633:toxicity; D012615:Sea Anemones
D003064:Cnidarian Venoms; D007202:Indicators and Reagents; D010446:Peptide Fragments; C032104:toxin III (Anemonia sulcata)