[Activation of human B lymphocytes by a particulate antigen].
A specific IgM antibody response toward the trinitrophenol (TNP) hapten can be induced in mononuclear blood cell suspensions upon culture with a particulate antigen: polyacrylamide beads conjugated with the TNP hapten (TNP-PAA). The response, and its specificity, are demonstrated by an increase in the number of TNP binding B lymphocytes (specific rosette forming cells), by the appearance of cells producing anti-TNP antibody at a high rate (haemolytic plaques), (ELISA test). The anti-TNP response requires monocytes, the role of which is to produce interleukin-1 (IL-1) and T lymphocytes (belonging to the T4 helper subset) the role of which is to produce interleukins (the characterization of which is under study). We propose a model or B cell activation based on the following signals: an early specific signal, provided by the particulate antigen; several non specific signals, provided by T derived interleukins. The anti-TNP response is negatively regulated by monocytes, the functional states of which can be modified in certain situations (autoimmunity, aging) or influenced by glucocorticoids. Suppressor T lymphocytes of this response (not exclusively of the T8 phenotype) can be induced and this can allow the evaluation of T suppressor cell function. This was used in adult idiopathic thrombocytopenic purpura treated with high doses of intra-venous gammaglobulins.
Galanaud
P
P
Delfraissy
J F
JF
Vazquez
A
A
Richard
Y
Y
Emilie
D
D
Boué
F
F
Wallon
C
C
Crevon
M C
MC
Dormont
J
J
D000180:Acrylic Resins / Q000276:immunology*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D006241:Haptens / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008954:Models, Biological; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D000099166:Polyacrylamides
D000180:Acrylic Resins; D006241:Haptens; D007075:Immunoglobulin M; C028795:trinitrophenyl polyacrylamide; D000099166:Polyacrylamides
Activation des lymphocytes B humains par un antigène particulaire.