Presence of tyrosine-O-sulfate in sheep pituitary prolactin.
When the metabolically obtained 35S-labelled sheep pituitary prolactin-rich fraction was subjected to chemical deglycosylation the radioactivity was retained in the immunoprecipitable prolactin. 35S-labelled prolactin-rich pituitary extract was fractionated on SDS-PAGE and protein was extracted from prolactin positive bands. When the extracted 35S-labelled prolactin was hydrolysed by alkali and then chromatographed on a thin layer of silica, it showed the presence of a radioactive compound which had an Rf value identical to the standard Tyr-O-SO4 synthesized and characterized in our laboratory.
Kohli
R
R
Department of Zoology, University of Delhi, India.
Chadha
N
N
Muralidhar
K
K
D000818:Animals; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D007163:Immunosorbent Techniques; D008698:Mesylates; D010902:Pituitary Gland / Q000032:analysis*; D011388:Prolactin / Q000032:analysis* / Q000378:metabolism; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D008698:Mesylates; D013431:Sulfates; D013462:Sulfur Radioisotopes; C005662:tyrosine O-sulfate; D014443:Tyrosine; D011388:Prolactin; C012077:trifluoromethanesulfonic acid