title: Comparative histochemical investigation of the glutathione S-transferase placental form and gamma-glutamyltranspeptidase during N-nitrosobis(2-hydroxypropyl)amine-induced pancreatic carcinogenesis in hamsters.
abstract: Immunohistochemical staining using anti-rat glutathione S-transferase placental form (GST-P) rabbit antibody and enzyme histochemical staining for gamma-glutamyltranspeptidase (gamma-GT) were investigated in putative preneoplastic lesions and adenocarcinomas in the pancreas of Syrian golden hamsters treated with N-nitrosobis(2-hydroxypropyl)amine (BHP). Areas with ductular proliferation, ductal hyperplasia, and intraductal carcinoma were strongly positive for GST-P binding and negative for gamma-GT. Cystic adenoma, microcarcinoma, and carcinomas were constantly positively stained by GST-P and partially positive for gamma-GT. GST-P appears to be useful as a positive marker for putative preneoplastic lesions in pancreatic carcinogenesis. Since normal acinar cells are strongly positive for gamma-GT, the findings might suggest that acinar cells contribute to the development of cystic adenoma, microcarcinoma, and carcinomas.
authors.forename: T
authors.initials: T
authors.lastname: Obara
authors.forename: T
authors.initials: T
authors.lastname: Makino
authors.forename: H
authors.initials: H
authors.lastname: Ura
authors.forename: Y
authors.initials: Y
authors.lastname: Yokose
authors.forename: T
authors.initials: T
authors.lastname: Kinugasa
authors.forename: M A
authors.initials: MA
authors.lastname: Moore
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Konishi
mesh_terms: D000818:Animals; D006224:Cricetinae; D005982:Glutathione Transferase / Q000032:analysis* / Q000276:immunology; D006651:Histocytochemistry; D008297:Male; D008647:Mesocricetus; D009602:Nitrosamines; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D010920:Placenta / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000032:analysis*
chemical_list: D009602:Nitrosamines; C012457:diisopropanolnitrosamine; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase