title: Simple fluorimetric screening for carazolol in swine kidneys by means of Sep-Pak cartridges.
abstract: A simple method to determine the beta-blocker carazolol in swine kidneys is presented. Spiked samples, homogenised under alkaline conditions, were heated. Passage of the ether extracts through C18 and silica Sep-Pak cartridges yielded a fraction containing the carazolol in 88% recovery. In this fraction carazolol was determined by fluorescence spectrophotometry. By this method a level of 1 microgram/kg in spiked samples can be detected. Injection of 10 micrograms/kg body weight, 105 minutes before slaughter, resulted in carazolol contents in kidneys ranging from 11 to 25 micrograms/kg (n = 8).
authors.forename: J W
authors.initials: JW
authors.lastname: Engelsma
authors.forename: J
authors.initials: J
authors.lastname: Simons
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000032:analysis; D000818:Animals; D007668:Kidney / Q000032:analysis*; D011412:Propanolamines / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D013552:Swine / Q000378:metabolism*
chemical_list: D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; C014382:carazolol