title: Quantitative determination of tertatolol in biological fluids by gas chromatography-mass spectrometry.
abstract: Quantitative determination of tertatolol concentrations in plasma and urine was performed by gas chromatography-mass spectrometry in the chemical-ionization mode with ammonia after successive extractions of the beta-blocking drug in alkaline, acid and final alkaline medium. [2H9]Tertatolol, isotopically stable under the operating conditions employed, was used as an internal standard, thus allowing quantities of 1 ng/ml to be specifically determined. Overall analytical error was less than 10%. Prior to isothermal chromatography at 240 degrees C on a column packed with 3% SE-30, both compounds were silylated with bis(trimethylsilyl)trifluoroacetamide. Detection was performed by monitoring the quasimolecular ions of tertatolol, m/z 368 and m/z 377, for the [2H9]tertatolol in the chemical-ionization mode with ammonia. The calibration curves obtained had linear characteristics for the concentration range 1-1125 ng/ml.
authors.forename: S
authors.initials: S
authors.lastname: Staveris
authors.forename: P
authors.initials: P
authors.lastname: Blaise
authors.forename: C
authors.initials: C
authors.lastname: Efthymiopoulos
authors.forename: M
authors.initials: M
authors.lastname: Schneider
authors.forename: G
authors.initials: G
authors.lastname: Jamet
authors.forename: L
authors.initials: L
authors.lastname: Jung
authors.forename: J C
authors.initials: JC
authors.lastname: Koffel
mesh_terms: D000284:Administration, Oral; D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000652:urine; D003510:Cyclohexanes; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007275:Injections, Intravenous; D011412:Propanolamines / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000652:urine; D012997:Solvents; D013876:Thiophenes
chemical_list: D000319:Adrenergic beta-Antagonists; D003510:Cyclohexanes; D011412:Propanolamines; D012997:Solvents; D013876:Thiophenes; C005632:tertatolol