On the transposition of copia-like nomadic elements in cultured Drosophila cells.
We studied the stability of the genomic distribution of six retrotransposon families in long-term and short-term cultures of Drosophila cells. In a subclone derived from Kc cells, no significant rearrangements were detected over an 8 year period. On the contrary, extensive reshuffling and amplification of transposon families were observed in recently established cell lines. These results show that in cultured Drosophila cells transposition appears to be restricted to the transition from the embryo to continuous cell lines.
Junakovic
N
N
U.A. CNRS 1135, Université Pierre et Marie Curie, Paris, France.
Di Franco
C
C
Best-Belpomme
M
M
Echalier
G
G
D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D004247:DNA