title: DNAase I footprinting of restriction enzymes.
abstract: DNAase I footprinting of restriction enzymes has been achieved by using calcium containing digestion buffers so that the enzymes bind to but do not cleave DNA. EcoR1 produces a footprint 17 bases long, overestimating the region of contact with DNA by about 7-8 base pairs. Restriction enzymes HaeIII and HinP1 generate smaller footprints of 15 and 13 base pairs respectively.
authors.affiliation: Dept. of Physiology and Pharmacology University of Southampton, U.K.
authors.forename: K R
authors.initials: KR
authors.lastname: Fox
mesh_terms: D002021:Buffers; D002118:Calcium; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015246:Deoxyribonuclease EcoRI; D003850:Deoxyribonuclease I / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific
chemical_list: D002021:Buffers; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D003850:Deoxyribonuclease I; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases; C024403:GGCC-specific type II deoxyribonucleases; D002118:Calcium