Bradykinin- and chemotactic peptide fMLP-stimulated leukotriene biosynthesis in rat lungs and its inhibition by vasoactive intestinal peptide.
The release/biosynthesis of peptidoleukotrienes (pLT) from rat lung tissue was stimulated with either bradykinin (Bk) or the chemotactic peptide formyl-methionyl-leucinyl-phenylalanine (fMLP). Bk- and fMLP-induced stimulation of pLT levels was dose-related for leukotriene D4 (LTD4), E4 (LTE4) and total pLTs and was maximal when a 10 microM concentration of either peptide was used. This stimulation was partially counteracted by vasoactive intestinal peptide (VIP), in agreement with the previously reported VIP inhibition of platelet activating factor- and IgG-stimulated pLT biosynthesis.
Di Marzo
V
V
Department of Biochemistry, Imperial College of Science & Technology, London.
Tippins
J R
JR
Morris
H R
HR
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D066298:In Vitro Techniques; D017999:Leukotriene E4; D015289:Leukotrienes / Q000096:biosynthesis*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000031:analogs & derivatives / Q000096:biosynthesis; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D015289:Leukotrienes; D013189:SRS-A; D014660:Vasoactive Intestinal Peptide; D009240:N-Formylmethionine Leucyl-Phenylalanine; D017999:Leukotriene E4; D001920:Bradykinin