Activation of washed chicken thrombocytes by 1-O-hexadecyl/octadecyl-2-acetyl-sn-glycero-3-phosphorylcholine (platelet-activating factor).
Washed, [3H]serotonin-labeled chicken thrombocytes aggregated and secreted [3H]serotonin when stimulated in vitro with platelet-activating factor (PAF), collagen and calcium ionophore A23187. The effective dose causing a 25% secretion of [3H]serotonin (ED25) from washed chicken thrombocytes was 10(-8) M for PAF, 5 X 10(-8) M for collagen and 3 X 10(-7) M for A23187. Chicken thrombocyte activation by PAF required Ca2+ and appeared to be mediated through a specific receptor for PAF.
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D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent; D002645:Chickens; D003094:Collagen / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000502:physiology*; D010974:Platelet Aggregation / Q000187:drug effects; D012701:Serotonin / Q000097:blood
D002413:Cations, Divalent; D010972:Platelet Activating Factor; D012701:Serotonin; D000001:Calcimycin; D004533:Egtazic Acid; D003094:Collagen; D008274:Magnesium; D002118:Calcium