Antibodies to beta 1 and beta 2 adrenoreceptors in Chagas' disease.
Evidence accumulated over the last decade concerning human and experimental models suggests that an immunopathological mechanism may be involved in the pathogenesis of chronic Chagas' disease. In this paper we demonstrate the existence of two different circulating IgG in chagasic patients which bind with myocardial beta 1 and spleen cell beta 2 adrenoceptors, acting as non-competitive inhibitors. Both chagasic IgG against beta 1 and beta 2 adrenoceptor increased intracellular levels of cAMP that could be blocked by specific beta 1 and beta 2 adrenoceptor antagonists. The specificity for beta 1 and beta 2 adrenoceptors and the independence of other tissue reactive antibodies was demonstrated by IgG absorption with turkey red blood cell (TRBC), human lymphocytes (HL) or guinea pig red blood cells (GPRBC). The F(ab')2 fraction acted similarly. This supports the specificity of beta 1 and beta 2 adrenoceptors to the chagasic IgG and the independence of the other tissue reactive antibodies, such as EVI system. The probable pathogenic role of both beta 1 and beta 2 adrenergic chagasic antibody is discussed.
Sterin-Borda
L
L
Centro de Estudios Farmacológicos y de Principios Naturales, Buenos Aires, Argentina.
Perez Leiros
C
C
Wald
M
M
Cremaschi
G
G
Borda
E
E
D000918:Antibody Specificity; D002598:Chagas Cardiomyopathy / Q000276:immunology*; D000242:Cyclic AMP / Q000378:metabolism; D004082:Dihydroalprenolol / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology; D009206:Myocardium / Q000276:immunology / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D000242:Cyclic AMP