[APH(3')-II phosphotransferase determination using protein transfer (western blotting)].
The APH(3')-II aminoglycoside-phosphotransferase has been studied by protein blotting. This technique allowed the detection of the enzyme in the crude extracts from clinical isolates of Enterobacteriaceae, and the molecular weight determination of the APH(3')-II. The obtained molecular weight of 25,000 is coincident with the previously reported value determined by a different method. These results demonstrate that this technique is useful for the study of aminoglycoside-modifying enzymes.
Rivera
M J
MJ
Departamento de Microbiología, Facultad de Medicina, Universidad de Zaragoza, España.
Pulido
D
D
Grasa
B
B
Gómez-Lus
R
R
D015153:Blotting, Western; D004926:Escherichia coli / Q000201:enzymology; D019868:Kanamycin Kinase; D008970:Molecular Weight; D010770:Phosphotransferases / Q000032:analysis*; D012477:Salmonella enteritidis / Q000201:enzymology
D010770:Phosphotransferases; D019868:Kanamycin Kinase
Estudio de la fosfotransferasa APH(3')-II mediante trasferencia de proteínas ("western blotting").