Quantitative determination of tertatolol in biological fluids by gas chromatography-mass spectrometry.
Quantitative determination of tertatolol concentrations in plasma and urine was performed by gas chromatography-mass spectrometry in the chemical-ionization mode with ammonia after successive extractions of the beta-blocking drug in alkaline, acid and final alkaline medium. [2H9]Tertatolol, isotopically stable under the operating conditions employed, was used as an internal standard, thus allowing quantities of 1 ng/ml to be specifically determined. Overall analytical error was less than 10%. Prior to isothermal chromatography at 240 degrees C on a column packed with 3% SE-30, both compounds were silylated with bis(trimethylsilyl)trifluoroacetamide. Detection was performed by monitoring the quasimolecular ions of tertatolol, m/z 368 and m/z 377, for the [2H9]tertatolol in the chemical-ionization mode with ammonia. The calibration curves obtained had linear characteristics for the concentration range 1-1125 ng/ml.
Staveris
S
S
Blaise
P
P
Efthymiopoulos
C
C
Schneider
M
M
Jamet
G
G
Jung
L
L
Koffel
J C
JC
D000284:Administration, Oral; D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000652:urine; D003510:Cyclohexanes; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007275:Injections, Intravenous; D011412:Propanolamines / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000652:urine; D012997:Solvents; D013876:Thiophenes
D000319:Adrenergic beta-Antagonists; D003510:Cyclohexanes; D011412:Propanolamines; D012997:Solvents; D013876:Thiophenes; C005632:tertatolol