title: Human polymorphonuclear leukocyte activating factor isolated from Aleurites Fordii Hemsl. (Euphorbiaceae) seed.
abstract: A human polymorphonuclear leukocyte (PMN) activating factor (AFH-S) was isolated from a tropical plant seed, Aleurites Fordii Hemsl. (AFH) in PBS. It is found that the AFH-S stimulate human PMNs and induced superoxide generation and chemotaxis. Superoxide generation was affected by the extracellular calcium ion or pretreatment with H-7 (PK-C inhibitor), but not by mepacrine (PLA2 inhibitor) or pertussis toxin (islet-activating protein: IAP). Furthermore, a lag time exists dose-dependently. In addition the cytosolic calcium was not increased by the stimulation with AFH-S. Thus, the receptor for AFH-S was suggested to be independent from Ni-like protein, PI-response, or PLA2-activation, and stimulate PMNs through activation of PK-C.
authors.affiliation: Department of Immunology, Kurume University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shichijo
authors.forename: K
authors.initials: K
authors.lastname: Shiotsuki
authors.forename: H
authors.initials: H
authors.lastname: Shibata
authors.forename: R
authors.initials: R
authors.lastname: Tsunosue
authors.forename: I
authors.initials: I
authors.lastname: Tsuboi
authors.forename: M
authors.initials: M
authors.lastname: Shiraishi
authors.forename: M M
authors.initials: MM
authors.lastname: Yokoyama
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D002118:Calcium / Q000378:metabolism; D002630:Chemotactic Factors / Q000302:isolation & purification* / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8; D007546:Isoquinolines / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D037342:Pertussis Toxin; D010879:Piperazines / Q000494:pharmacology; D011796:Quinacrine / Q000494:pharmacology; D012639:Seeds / Q000276:immunology; D013481:Superoxides / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D002630:Chemotactic Factors; D016209:Interleukin-8; D007546:Isoquinolines; D010455:Peptides; D010879:Piperazines; D010566:Virulence Factors, Bordetella; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D037342:Pertussis Toxin; D011796:Quinacrine; D002118:Calcium