Detection of human papillomavirus in normal and dysplastic tissue by the polymerase chain reaction.
Detection of human papilloma virus (HPV) types 16 and 18 in formalin-fixed, paraffin-embedded tissue by a new in vitro DNA amplification method, the polymerase chain reaction, was compared with detection with genomic DNA probes using in situ hybridization. The polymerase chain reaction replicates exponentially HPV DNA sequences present in a single 5- to 10-micron paraffin-embedded tissue section. The amplified sequences are detected with a DNA hybridization probe in a dot blot assay. The HPV polymerase chain reaction was able to detect on the average less than one HPV genome/cell as determined by tests of paraffin sections of cell pellets with known HPV genomic content. Cervical sections from 21 patients with HPV types 16, 18, or 31 as determined by in situ DNA hybridization were analyzed by the polymerase chain reaction. No disagreements between the two methods were detected. The sections comprising normal and dysplastic epithelium were further analyzed by the HPV polymerase chain reaction. The presence of virus correlated with the presence of dysplasia in the sections, though 3 of 10 normal sections contained HPV, and 1 of 21 sections with dysplasia lacked HPV 16 or 18. The polymerase chain reaction can specifically detect HPV 16 or 18 with high sensitivity from paraffin-embedded tissues.
Shibata
D
D
Department of Pathology, Los Angeles County-University of Southern California Medical Center.
Fu
Y S
YS
Gupta
J W
JW
Shah
K V
KV
Arnheim
N
N
Martin
W J
WJ
D001345:Autoradiography; D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D002584:Cervix Uteri / Q000382:microbiology*; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002578:Uterine Cervical Dysplasia / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology
D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase