title: A new type of coated vesicular carrier that appears not to contain clathrin: its possible role in protein transport within the Golgi stack.
abstract: Isolated Golgi membranes incubated in the presence of ATP and a cytosolic protein fraction form a population of coated buds or vesicles from the Golgi cisternae. The coats do not have the characteristic hexagonal-pentagonal basketwork of clathrin, and do not react with anti-clathrin polyclonal antibody. The conditions that produce these apparently nonclathrin-coated buds also reconstitute protein transport between compartments of the Golgi stack. The membrane of the buds contains the glycoprotein in transit through these Golgi stacks (VSV-encoded G protein). This suggests that protein transport through the Golgi stack is mediated by a new type of coated vesicle that does not contain clathrin. The concentration of G protein in the coated buds reflects the local concentration of G protein in the cisternae, raising the possibility that the Golgi coated vesicles may be "bulk" membrane carriers.
authors.forename: L
authors.initials: L
authors.lastname: Orci
authors.forename: B S
authors.initials: BS
authors.lastname: Glick
authors.forename: J E
authors.initials: JE
authors.lastname: Rothman
mesh_terms: D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D002966:Clathrin / Q000502:physiology*; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism* / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D011992:Endosomes / Q000378:metabolism*; D005260:Female; D006056:Golgi Apparatus / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D008562:Membrane Glycoproteins; D010053:Ovary; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D002966:Clathrin; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins