title: Enhancement of Epstein-Barr virus gene expression by other herpesviruses.
abstract: We examined the effect of infection by other herpesviruses on the induction of Epstein-Barr virus (EBV) antigens. Raji cells were infected with a variety of viruses before superinfection with P3HR-1 virus 6 h later. Much earlier and greater synthesis of EBV antigens was observed in herpesvirus (herpes simplex virus type 1, type 2 and pseudorabies virus)-infected Raji cells than in mock-infected Raji cells prior to superinfection with P3HR-1 virus, as determined by indirect immunofluorescence tests or immunoprecipitation and PAGE. Although infection with a temperature-sensitive mutant of pseudorabies virus (tsG1) enhanced EBV antigen synthesis as well as wild-type pseudorabies virus at permissive temperature in Raji cells superinfected with P3HR-1 virus, EBV antigen synthesis was strongly inhibited at temperatures nonpermissive for tsG1. These results suggest that a herpesvirus immediate-early protein may play a role in enhancement of EBV antigen expression.
authors.affiliation: Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey 17033.
authors.forename: H
authors.initials: H
authors.lastname: Kikuta
authors.forename: F
authors.initials: F
authors.lastname: Rapp
mesh_terms: D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics; D002051:Burkitt Lymphoma; D036022:Capsid Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006564:Herpesviridae / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007700:Kinetics; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C041788:Epstein-Barr viral capsid antigen; C033305:Epstein-Barr virus early antigen