title: The structure of a plasmid of Chlamydia trachomatis believed to be required for growth within mammalian cells.
abstract: Sequence analysis of a 7.5 kb DNA plasmid isolated from Chlamydia trachomatis shows 8 open reading frames (ORFs) regularly spaced along most of the sequence. One of these ORFs encodes a 451-amino-acid polypeptide highly homologous to the DnaB protein of Escherichia coli. A region between ORFs 6 and 7 contains a cluster of alternating ATs and a 22 bp sequence tandemly repeated 4 times, suggesting a replication control region. Several ORFs correspond to plasmid-specific polypeptides that have been described. Codons ending with A or T are more frequent, as might be expected from the high A/T content (64%) of the plasmid, and codon usage is similar to that of the C. trachomatis chromosomal gene, omp1L2.
authors.affiliation: Sclavo Research Centre, Siena, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Comanducci
authors.forename: S
authors.initials: S
authors.lastname: Ricci
authors.forename: G
authors.initials: G
authors.lastname: Ratti
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D003062:Codon; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes