Prospective evaluation of a monoclonal antibody in diagnosis of Pneumocystis carinii pneumonia.
To evaluate the ability of a mouse monoclonal antibody, 2G2, directed against human Pneumocystis carinii, to detect the organism in clinical specimens, a prospective study of the antibody in an indirect immunofluorescent assay was undertaken. P carinii was rapidly detected in thirteen of fourteen bronchoalveolar lavage specimens positive by toluidine-blue-O stain, none of eleven lavage specimens negative by toluidine-blue-O, neither of two impression smears of histologically negative open-lung biopsy specimens, and both of two impression smears of histologically positive necropsy specimens. Immunofluorescence with monoclonal antibody 2G2 is a rapid, simple, and specific technique for detection of P carinii in clinical specimens.
Kovacs
J A
JA
Gill
V
V
Swan
J C
JC
Ognibene
F
F
Shelhamer
J
J
Parrillo
J E
JE
Masur
H
H
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000818:Animals; D000911:Antibodies, Monoclonal; D001980:Bronchi / Q000382:microbiology; D001999:Bronchoscopy; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D011010:Pneumocystis / Q000276:immunology; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000276:immunology; D011446:Prospective Studies; D014048:Tolonium Chloride
D000911:Antibodies, Monoclonal; D014048:Tolonium Chloride