Structure of the androgen dependent SVS VI protein as derived from cDNA.
The 11S poly(A+)RNA from rat seminal vesicle was cloned. By hybrid selection of clones reacting to the low molecular weight region of the 11S peak, a clone containing a new seminal vesicle cDNA sequence called SVS VI was isolated. The DNA sequence of two overlapping cDNAs (pSV24 and pSV33) is presented. The sequence of SVS VI was compared to the previously isolated SVS IV and SVS V cDNAS. Dot hybridization showed that SVS VI is androgen responsive after giving testosterone to castrated rats. The hydrophilicity was analyzed using standard Bionet procedures. All three proteins are extremely variable, rich in alpha-helix and very water soluble. The computer predicted hydrophilicity is compared for SVS VI, V and IV. A small region in the 3'-non-coding area of SVS VI has high similarity to a region in SVS IV mRNA.
Schifman
A L
AL
W. Alton Jones Cell Science Center, Inc., Lake Placid, NY 12946.
Mansson
P E
PE
Carter
D B
DB
Yamada
K
K
Harris
M M
MM
Harris
S E
SE
D000595:Amino Acid Sequence; D000728:Androgens / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004251:DNA Transposable Elements; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008969:Molecular Sequence Data; D029584:Prostatic Secretory Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000502:physiology; D012967:Sodium Dodecyl Sulfate; D013739:Testosterone / Q000494:pharmacology
D000728:Androgens; D004251:DNA Transposable Elements; D029584:Prostatic Secretory Proteins; D011506:Proteins; D012333:RNA, Messenger; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D012967:Sodium Dodecyl Sulfate; D013739:Testosterone; D004247:DNA