A novel oncogene related to c-mil is transduced in chicken neuroretina cells induced to proliferate by infection with an avian lymphomatosis virus.
Non-dividing neuroretina cells from chicken embryos are induced to proliferate after a long latency, following infection with Rous associated virus type 1, an avian retrovirus which does not carry a transforming gene. We have isolated from these proliferating cells an acutely mitogenic retrovirus, designated IC10, which contains a novel oncogene. Nucleotide sequencing showed that the IC10 virus has transduced 1101 nucleotides of cellular origin inserted between the gag and env genes of RAV-1. This oncogene, designated v-Rmil, is 70.1% homologous to v-mil. v-Rmil encodes a protein of 40,976 daltons sharing 83.8% homology with the catalytic domain of the v-mil protein. Divergence with the v-mil gene product is observed at the NH2- and COOH-terminal portions of the v-Rmil protein. Restriction analysis of normal chicken DNA indicated that v-Rmil is derived from a cellular gene distinct from c-mil. The c-Rmil gene is transcribed through a major mRNA, greater than 10 kb in length, that is detected at much higher levels in neuroretinas, as compared to other embryonic tissues.
Marx
M
M
Institut Curie-Biologie, Centre Universitaire, Orsay, France.
Eychène
A
A
Laugier
D
D
Béchade
C
C
Crisanti
P
P
Dezélée
P
P
Pessac
B
B
Calothy
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001354:Avian Leukosis Virus / Q000502:physiology*; D001483:Base Sequence; D015139:Blotting, Southern; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D027384:Oncogene Proteins v-raf; D009857:Oncogenes; D015183:Restriction Mapping; D012191:Retroviridae Proteins / Q000235:genetics*; D014161:Transduction, Genetic
D012191:Retroviridae Proteins; D004247:DNA; D027384:Oncogene Proteins v-raf; D015246:Deoxyribonuclease EcoRI