[Partial purification of endogenous inhibitors of (+)-[3H] SKF 10047 binding with sigma-opioid receptors of the liver].
Porcine liver extract inhibits (+)-[3H]SKF 10047 binding to rat liver membranes. The inhibitors of (+)-[3H]SKF 10047 binding were detected in acetone supernatant and in acid acetonated powder of the liver extract. Partial purification of the acetone supernatant revealed some characteristics of these inhibitors: low molecular weight (less than 1000), thermostability, resistance to pronase digestion, solubility in water and organic solvents.
Nagornaia
L V
LV
Samovilova
N N
NN
Korobov
N V
NV
Vinogradov
V A
VA
D000096:Acetone; D000818:Animals; D002850:Chromatography, Gel; D008024:Ligands; D008099:Liver / Q000378:metabolism*; D010620:Phenazocine / Q000031:analogs & derivatives* / Q000378:metabolism; D011869:Radioligand Assay; D011957:Receptors, Opioid / Q000302:isolation & purification* / Q000378:metabolism; D017480:Receptors, sigma; D013552:Swine
D008024:Ligands; D011957:Receptors, Opioid; D017480:Receptors, sigma; D000096:Acetone; C012134:SK&F 10047; D010620:Phenazocine
Chastichnaia ochistka éndogennykh ingibitorov sviazyvaniia (+)-[3H] SKF 10047 s sigma-opioidnymi retseptorami pecheni.