title: The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line.
abstract: When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10-20% of the cytochrome P-450-dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP-glucuronyltransferase and glutathione-S-transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O-dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50-, 30- and 10-fold, respectively.
authors.affiliation: Department of Medicine & Therapeutics, University of Aberdeen, Scotland.
authors.forename: H
authors.initials: H
authors.lastname: Doostdar
authors.forename: S J
authors.initials: SJ
authors.lastname: Duthie
authors.forename: M D
authors.initials: MD
authors.lastname: Burke
authors.forename: W T
authors.initials: WT
authors.lastname: Melvin
authors.forename: M H
authors.initials: MH
authors.lastname: Grant
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002460:Cell Line; D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*
chemical_list: D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase; D005978:Glutathione