title: [Regulation of the shape and size of human erythrocytes by protein kinases A and C].
abstract: The effect of 12-O-tetradecanoyl-phorbol-12-acetate (TPA) and dibutyryl-3,5-cAMP on the shape, volume and protein phosphorylation in human red blood cells (RBC) was studied. TPA (but not cAMP) reduced the average volume of RBC and their transformed definite pool in the cup-shaped form. The changes in the physical parameters were accompanied by an increase in the cytoskeleton protein phosphorylation. An additive effect of cAMP and TPA on the phosphorylation of bands 4.1 and 4.9 was established, thus indicating that distinct domains of amino acid residues were phosphorylated by these stimuli. It was concluded that protein kinase C regulates the shape and volume of human RBC. A model, in which the state of spectrin 4.1-actin and actin 4.9-myosin complexes define the shape and volume of RBC and thus influence ion transport, is proposed.
authors.forename: G M
authors.initials: GM
authors.lastname: Kravtsov
authors.forename: N O
authors.initials: NO
authors.lastname: Dulin
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Kotelevtsev
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Postnov
mesh_terms: D003994:Bucladesine / Q000494:pharmacology; D004789:Enzyme Activation; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000097:blood; D010750:Phosphoproteins / Q000097:blood; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins; D003994:Bucladesine; D011494:Protein Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate
vernacular_title: Reguliatsiia formy i ob''ema éritrotsitov cheloveka proteinkinazami A i C.