Prevention by antioxidants of the hemolysis of erythrocytes of cattle, pigs and humans treated with t-butyl hydroperoxide.
Urate, 3-ribosylurate, ascorbate, glutathione and plasma protected bovine, porcine and human erythrocytes from hemolysis caused by t-butyl hydroperoxide (t-BHP). Urate partially protected porcine erythrocytes from hemolysis by t-BHP when it was added 15 min after the addition of the t-BHP, but it did not protect when added 30 min after the t-BHP. Glutathione and ascorbate protected oxyhemoglobin from oxidation to methemoglobin by t-BHP; 3-ribosylurate gave only slight protection. Urate stimulated the formation of methemoglobin from oxyhemoglobin during treatment with t-BHP.
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D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D005978:Glutathione / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D008297:Male; D008706:Methemoglobin / Q000378:metabolism; D010545:Peroxides / Q000494:pharmacology*; D012263:Ribonucleosides / Q000494:pharmacology; D013045:Species Specificity; D013552:Swine; D014527:Uric Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D000975:Antioxidants; D010545:Peroxides; D012263:Ribonucleosides; C012090:uric acid riboside; D014527:Uric Acid; D008706:Methemoglobin; D020122:tert-Butylhydroperoxide; D005978:Glutathione; D001205:Ascorbic Acid