[Use of a rapid method for determining primary infection by rubella virus].
The assay of specific immunoglobulin M (IgM) antibodies could aid in the diagnosis of early viral infection. Here we described the results obtained for IgG and IgM separation on a small column of Sephadex A-50 ion exchanger and the subsequent measure of hemagglutination inhibition antibodies for rubella virus. This diagnostic procedure combines the efficiency, rapidity and simplicity of a chromatography method with the detection of specific antibodies by reference method. The average immunoglobulin recuperation was 91% for the immunoglobulin G (IgG) and 70% for the IgM. Eighteen sera obtained from patients with presumptive diagnostic compatible with primary rubella infection, 10 from patients with known immune status for rubella virus, and 3 sera with specific IgM for BK virus, were comparatively studied by gel filtration. Sephadex G-200, enzyme immunoassay for rubella IgM antibody and Sephadex A-50 ion exchanger. The results indicated that the proposed method can be satisfactorily used for the diagnostic of primary rubella virus infection.
Nates
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Instituto de Virología Dr. José M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Ferreira Centeno
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Márquez
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Zapata
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M
D000914:Antibodies, Viral / Q000032:analysis*; D001739:BK Virus / Q000276:immunology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D012409:Rubella / Q000175:diagnosis*; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C040901:rubella antibodies
Utilización de un método rápido para determinar infección primaria por virus rubéola.