Selective extraction of haemagglutinin and matrix protein from Sendai virions by employing trifluoperazine as a detergent.
Incubation of trifluoperazine, a local anaesthetic, at concentrations higher than the cmc with Sendai virus particles produces the selective solubilization of the haemagglutinin neuraminidase (HN) and matrix (M) proteins. This phenomenon involves aggregation of the Sendai virions and therefore the separation of HN and M from the rest of the particle can be performed by bench centrifugation. The supernatant contains the HN and M proteins and HN, once inserted into liposomes, elicits its own biological activities. Therefore, the method seems suitable for purifying large amounts of HN.
Baiocchi
M
M
Laboratorio di Biologia Cellulare, Istituto Superiore di Sanita, Roma, Italy.
Pescarmona
M
M
Bruschi
M L
ML
Montecucco
C
C
Squatriti
T
T
Tomasi
M
M
D000818:Animals; D002642:Chick Embryo; D003902:Detergents; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D009439:Neuraminidase / Q000302:isolation & purification; D010222:Parainfluenza Virus 1, Human / Q000032:analysis* / Q000201:enzymology; D012995:Solubility; D014268:Trifluoperazine; D014763:Viral Matrix Proteins / Q000302:isolation & purification*; D014771:Virion / Q000032:analysis* / Q000201:enzymology
D003902:Detergents; D006389:Hemagglutinins, Viral; D014763:Viral Matrix Proteins; D014268:Trifluoperazine; D009439:Neuraminidase