title: Sialyltransferase-1 in a human malignant glioma cell line. Kinetic characteristics and effect of human interferon-beta.
abstract: Sialyltransferase-1 activity was studied in cultured 12-18 human glioma cells. The apparent Km and Vmax with variable LacCer concentrations were 32 microM and 197 pmoles/mg protein/hr and with variable CMP-NeuAc concentrations were 172 microM and 877 pmoles/mg protein/hr., respectively. The pH optimum towards exogenous LacCer was 6.0 and towards endogenous acceptors was 6.2. The optimum protein:detergent ratio was 1:1. Human beta interferon (1000 units/ml medium) increased sialyltransferase-1 activity only slightly on a protein basis but increased it 47% on a per cell basis. These results demonstrate that one of the biochemical effects of beta-interferon on 12-18 human glioma cells is to stimulate ganglioside synthesis.
authors.affiliation: Department of Pathology, College of Medicine, Ohio State University, Columbus 43210.
authors.forename: A J
authors.initials: AJ
authors.lastname: Yates
authors.forename: J F
authors.initials: JF
authors.lastname: Collins
authors.forename: P J
authors.initials: PJ
authors.lastname: Elder
authors.forename: R E
authors.initials: RE
authors.lastname: Stephens
mesh_terms: D002460:Cell Line; D005909:Glioblastoma / Q000201:enzymology*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007700:Kinetics; D011994:Recombinant Proteins / Q000494:pharmacology*; D012799:Sialyltransferases / Q000378:metabolism*
chemical_list: D007370:Interferon Type I; D011994:Recombinant Proteins; D012799:Sialyltransferases