title: Purification and crystallization of thymidine kinase from herpes simplex virus type 1.
abstract: Thymidine kinase from herpes simplex virus type 1 (ATP:thymidine 5'-phosphotransferase; EC 2.7.1.21) has been purified from an overexpression system and crystallized against ammonium sulfate by using the hanging-drop technique. The tetragonal crystals are of space group P4122 or P4322, and have unit cell dimensions a = b = 84 A, c = 180 A.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CN 06511.
authors.forename: M R
authors.initials: MR
authors.lastname: Sanderson
authors.forename: P S
authors.initials: PS
authors.lastname: Freemont
authors.forename: H M
authors.initials: HM
authors.lastname: Murthy
authors.forename: J F
authors.initials: JF
authors.lastname: Krane
authors.forename: W C
authors.initials: WC
authors.lastname: Summers
authors.forename: T A
authors.initials: TA
authors.lastname: Steitz
mesh_terms: D003460:Crystallization; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D013937:Thymidine Kinase