Purification of tick-borne encephalitis virus in tris-modified porous glasses.
Macroporous glasses, chemically modified by tris-hydroxymethyl-aminomethane, were used for gel-permeation chromatography of tick-borne encephalitis (TBE) virus suspension. The highest yield of purified virus was observed in the carriers containing not less than 0.01 micrograms-equivalent/m2 surface-attached tris-hydroxymethyl-aminomethane groups at pH of the eluent higher than 7.6. The obtained modified porous carriers appeared to be suitable for the purification of both infectious and inactivated preparations of TBE virus.
Krasilnikov
I V
IV
Elbert
L B
LB
Borisova
V N
VN
Nakhapetyan
L A
LA
D000818:Animals; D001921:Brain / Q000382:microbiology; D002478:Cells, Cultured; D004669:Encephalitis Viruses, Tick-Borne / Q000254:growth & development / Q000302:isolation & purification*; D004675:Encephalitis, Tick-Borne / Q000382:microbiology; D005898:Glass; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D007700:Kinetics; D051379:Mice; D013552:Swine; D014325:Tromethamine
D014325:Tromethamine