title: Long-term regulation of tyrosine hydroxylase activity in the superior cervical ganglion in organ culture: effects of nerve stimulation and dexamethasone.
abstract: The rat superior cervical ganglion was stimulated in vitro via its preganglionic trunk and then maintained in organ culture for 2-3 days. Following nerve stimulation, the specific activity of tyrosine hydroxylase (TH) increased with a lag period of at least 12 h, and this increased enzyme activity was maintained throughout the culture period. The magnitude of the increase in TH activity depended on the frequency and duration of preganglionic nerve stimulation. This transsynaptic increase in enzyme activity could be completely blocked by the nicotinic antagonist, hexamethonium. The synthetic glucocorticoid dexamethasone, at concentrations above 1 nM, increased TH activity in unstimulated ganglia with a lag period of at least 24 h. The increase in enzyme activity produced by dexamethasone was not affected by nicotinic antagonists or by prior decentralization of the superior cervical ganglion, in contrast to the findings of previous workers. These data indicate that dexamethasone can stimulate ganglionic TH activity in the absence of cholinergic stimulation. In addition, experiments combining dexamethasone with nerve stimulation demonstrate that the steroid can potentiate the effects on TH activity of brief periods of nerve stimulation.
authors.forename: N Y
authors.initials: NY
authors.lastname: Ip
authors.forename: R E
authors.initials: RE
authors.lastname: Zigmond
mesh_terms: D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D004558:Electric Stimulation; D005728:Ganglia, Sympathetic / Q000201:enzymology* / Q000502:physiology; D008297:Male; D009474:Neurons / Q000201:enzymology; D009924:Organ Culture Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
chemical_list: D003907:Dexamethasone; D014446:Tyrosine 3-Monooxygenase