Immunological characterization of bovine lysyl oxidase.
Antibodies to homogeneously purified bovine aortic lysyl oxidase were prepared in chickens. The chicken anti-lysyl oxidase antiserum effectively inhibited bovine aortic lysyl oxidase activity. Non-immune antiserum from chickens, goats and humans was found to enhance bovine aortic lysyl oxidase activity, while non-immune rabbit serum inhibited enzyme activity. A competitive ELISA was developed to monitor immunoreactive lysyl oxidase during purification. Chromatography of bovine lysyl oxidase on Sephacryl S-200, the final step in purification, revealed two peaks of immunoreactive lysyl oxidase. The large molecular weight peak appears to contain inactive multimeric forms of the enzyme.
Burbelo
P D
PD
Kagan
H M
HM
Chichester
C O
CO
D000594:Amino Acid Oxidoreductases / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D001011:Aorta / Q000201:enzymology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D008970:Molecular Weight; D008249:Protein-Lysine 6-Oxidase / Q000276:immunology* / Q000302:isolation & purification
D000594:Amino Acid Oxidoreductases; D008249:Protein-Lysine 6-Oxidase