title: Nucleotide sequence of the gene encoding the two-subunit pilin of Bacteroides nodosus 265.
abstract: The nucleotide sequence of the gene encoding pilin from Bacteroides nodosus 265 has been determined. The pilin is encoded by a single-copy gene, from which can be predicted a prepilin comprising a single protein chain of Mr 16,637. The prepilin sequence differs in several respects from the mature protein sequence. Seven additional N-terminal amino acid residues are present in prepilin, whereas residue 8, phenylalanine, undergoes posttranslational modification to become the N-methylated amino-terminal residue of mature pilin. In addition, further processing occurs through internal cleavage to produce two noncovalently linked subunits characteristic of pilins from serogroup H of B. nodosus, of which strain 265 is a member. The position of cleavage has been identified between alanine residues at positions 72 and 73 of the mature 149-residue pilin protein. The predicted pilin sequence of B. nodosus 265 shows extensive N-terminal amino acid sequence homology with other pilins of the N-methylphenylalanine type. In addition this sequence also shows homology with these N-methylphenylalanine-type pilins in the C-terminal region of the molecule, especially with pilin from Pseudomonas aeruginosa PAK.
authors.forename: T C
authors.initials: TC
authors.lastname: Elleman
authors.forename: P A
authors.initials: PA
authors.lastname: Hoyne
authors.forename: N M
authors.initials: NM
authors.lastname: McKern
authors.forename: D J
authors.initials: DJ
authors.lastname: Stewart
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001439:Bacteroides / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial / Q000235:genetics; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial; D005796:Genes; D005798:Genes, Bacterial; D011498:Protein Precursors / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003062:Codon; D004269:DNA, Bacterial; D011498:Protein Precursors; D036781:Fimbriae Proteins