[Effect of energy metabolism on the ionic selectivity of the K+ center of the Na+/K+ pump in the skin of the frog].
The uptake of Rb+ and Tl+ in frog skin incubated in saline containing 86Rb and 204Tl was studied. The ratio of ouabain-sensitive influxes Tl+/Rb+ = 2. Inhibition of the unidirectional transport of Na+ by rotenone or by Tl+ was not accompanied by the total decrease in the ouabain-sensitive uptake of Rb+ and Tl+. A substantial number of the Na+/K+ pumps continued to operate to maintain the intracellular ion homeostasis. The Tl+/Rb+ ratio of the cation influxes via pumps of this group was about twice as high compared to the control values. A coexistence of the two forms of Na+/K+ pumps in frog skin epithelium was postulated. They differ in both ion selectivity and the source of energy (respiration or glycolysis). The Tl+/Rb+ ratio of the ouabain-insensitive fluxes seems to be independent of the energetic metabolism.
Skul'skiĭ
I A
IA
Lapin
A V
AV
D000332:Aerobiosis / Q000187:drug effects; D000693:Anaerobiosis / Q000187:drug effects; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D011896:Rana temporaria; D012402:Rotenone / Q000494:pharmacology; D012414:Rubidium Radioisotopes; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism*; D013794:Thallium Radioisotopes
D015221:Potassium Channels; D012414:Rubidium Radioisotopes; D015222:Sodium Channels; D013794:Thallium Radioisotopes; D012402:Rotenone; D010042:Ouabain; D011188:Potassium
Vliianie énergeticheskogo metabolizma na ionnuiu selektivnost' K+-tsentra Na+/K+-nasosa v kozhe liagushki.