Vanadate-stimulated NADH oxidation requires polymeric vanadate, phosphate and superoxide.
NADH oxidation, catalyzed by the microsomal enzyme system is stimulated on addition of polymeric vanadate. Maximum stimulation by polymeric vanadate was obtained in the presence of phosphate buffer. The small stimulation obtained by metavanadate (500 microM) increased on acidification followed by neutralization, or on adding a trace amount of polymeric vanadate (1 microM).
Patole
M S
MS
Department of Biochemistry, Indian Institute of Science, Bangalore.
Gullapalli
S
S
Ramasarma
T
T
D000818:Animals; D006863:Hydrogen-Ion Concentration; D008861:Microsomes / Q000201:enzymology; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010710:Phosphates / Q000378:metabolism*; D011108:Polymers; D051381:Rats; D013481:Superoxides / Q000378:metabolism*; D014638:Vanadates / Q000378:metabolism*
D010710:Phosphates; D011108:Polymers; D009243:NAD; D013481:Superoxides; D014638:Vanadates