Host origin of marrow stromal cells obtained from marrow transplant recipients and transformed in vitro by simian virus-40.
We evaluated the karyotypes of marrow-derived stromal cell lines established by simian virus-40 (SV-40) transformation of long-term cultures from four men who received marrow transplants from women donors. In all cases a normal female karyotype was found in marrow cells. In contrast, a Y chromosome was identified in metaphase cells from the stromal lines, suggesting that following successful marrow transplantation, cells in the marrow microenvironment susceptible to SV-40 transformation remain host in origin.
Raskind
W H
WH
Department of Medicine, University of Washington School of Medicine, Seattle 98195.
Singer
J W
JW
Morgan
C A
CA
Fialkow
P J
PJ
D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002472:Cell Transformation, Viral; D003238:Connective Tissue / Q000637:transplantation; D003239:Connective Tissue Cells; D006801:Humans; D007621:Karyotyping; D008297:Male; D012727:Sex Characteristics; D013539:Simian virus 40; D014960:X Chromosome / Q000648:ultrastructure; D014998:Y Chromosome / Q000648:ultrastructure