title: Multistress resistance of Saccharomyces cerevisiae is generated by insertion of retrotransposon Ty into the 5' coding region of the adenylate cyclase gene.
abstract: Heat shock-resistant mutants, which were isolated by their ability to withstand lethal heat treatment, were characterized. Resistance was demonstrated to be a consequence of insertion of retrotransposon Ty into either the 5' coding or noncoding region, close to the putative initiation codon of the adenylate cyclase gene CYR1 (or CDC35). These heat shock-resistant mutants contained about threefold lower adenylate cyclase activity than wild-type strains. The mutants were also observed to be resistant to other stresses such as UV light and ethanol. These results demonstrate that multistress resistance, which may confer a survival advantage to yeast cells, can be generated by transposition of a Ty element into CYR1.
authors.affiliation: Department of Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
authors.forename: H
authors.initials: H
authors.lastname: Iida
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics*; D004251:DNA Transposable Elements / Q000187:drug effects / Q000528:radiation effects; D000431:Ethanol / Q000494:pharmacology; D005796:Genes; D005800:Genes, Fungal; D005838:Genotype; D006358:Hot Temperature; D009154:Mutation; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004251:DNA Transposable Elements; D000431:Ethanol; D000262:Adenylyl Cyclases