Kinetics and specificity at the clonal level of the cytotoxic T lymphocyte response to influenza pneumonia.
We have studied the kinetics and specificity of the cytotoxic T lymphocyte (CTL) response to influenza A/PR/8 (H1N1) virus pulmonary infection in the mouse detected using spleen cells from infected mice which were stimulated in bulk and limiting dilution cultures. A hybrid protein designated D-peptide, which contains the terminal 157 amino acids of the HA2 subunit of A/PR/8 virus, was used to stimulate influenza virus subtype-specific secondary CTL in vitro. Infection induced two specificities of precursor CTL, cross-reactive and subtype-specific. The kinetics of the subtype-specific CTL response detected by the D-peptide were similar to the cross-reactive CTL response detected by stimulation with live virus. The majority of the precursor CTL (CTL-p) are able to lyse virus-infected target cells in a cross-reactive fashion. The number of memory subtype-specific and cross-reactive CTL increased by approximately 2.5 logs10 during the first 3 weeks after infection.
Jaffe
P A
PA
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655.
Kuwano
K
K
Yamada
A
A
Scott
M
M
Young
J F
JF
Ennis
F A
FA
D000281:Administration, Intranasal; D000818:Animals; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D009980:Influenza A virus / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D011024:Pneumonia, Viral / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*; D013997:Time Factors; D014760:Viral Fusion Proteins / Q000378:metabolism
D014760:Viral Fusion Proteins