Neurotensin binds with high affinity to small cell lung cancer cells.
Several small cell lung cancer cell lines bound 125I-neurotensin with high affinity. Radiolabeled neurotensin bound with high affinity (Kd = 4 nM) to a single class of sites (2300/cell) using cell line NCI-H209. Binding was time dependent and reversible. Pharmacology studies indicated that the C-terminal of neurotensin was important for the high affinity binding activity. Because high concentrations of neurotensin and its receptors are associated with small cell lung cancer, neurotensin may function as a regulatory peptide in this disease.
Allen
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AE
Department of Biochemistry, George Washington University School of Medicine and Health Sciences, Washington, DC 20037.
Carney
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DN
Moody
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TW
D001667:Binding, Competitive; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D009496:Neurotensin / Q000378:metabolism*; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter; D009496:Neurotensin