Expression of an immunoglobulin kappa light-chain gene in lymphoid cells using a bovine papillomavirus-1 (BPV-1) vector.
Bovine papillomavirus-1 (BPV-1) replicates extrachromosomally in certain murine cell lines, suggesting that vectors based on the BPV-1 replicon might provide a means of obtaining more uniform gene expression among independent transformants. We have tested such a vector for the expression in hybridoma cells of the immunoglobulin kappa light-chain gene, but found that the level of expression varies greatly among transformants. Our results also indicate that in these transformants the vector has probably been incorporated into chromosomal DNA.
Baker
M D
MD
Department of Immunology, University of Toronto, Ontario, Canada.
Murialdo
H
H
Shulman
M J
MJ
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D005803:Genes, Immunoglobulin; D005822:Genetic Vectors; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D027383:Papillomaviridae / Q000235:genetics*; D010954:Plasmacytoma; D014162:Transfection
D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains