Cytoplasmic pH in the action of epidermal growth factor (EGF) in cultured porcine thyroid cells.
We demonstrate measurement of cytoplasmic pH (pHi), using 2',7'-bis(2-carboxyethyl)-5 (and 6-) carboxyfluorescein (BCECF), and internalized fluorescent pHi indicator, in thyroid cells. Using cultured porcine thyroid cells, we studied the effects of epidermal growth factor (EGF) on pHi and [3H] thymidine incorporation; 10 nM EGF alkalinizes thyroid cells and stimulates thymidine incorporation. The results indicate that Na+/H+ exchange or cell alkalinization may function as a transmembrane signal transducer in the action of EGF in the thyroid cells.
Takasu
N
N
Department of Gerontology, Endocrinology and Metabolism, School of Medicine, Shinshu University, Nagano-ken, Japan.
Nagasawa
Y
Y
Komiya
I
I
Yamada
T
T
Shimizu
Y
Y
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D017923:Sodium-Hydrogen Exchangers; D013552:Swine; D013961:Thyroid Gland / Q000502:physiology*; D013997:Time Factors
D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D004815:Epidermal Growth Factor; D004247:DNA