Detection and characterization of the protein encoded by the chicken c-rel protooncogene.
We have identified the protein encoded by chicken c-rel, the cellular homolog of the v-rel oncogene carried by reticuloendotheliosis virus. The protein has been detected in two avian lymphoid cell lines and in chick embryo fibroblasts. It has a molecular weight of 68,000, suggesting that the viral gene (which encodes a protein of 59,000 molecular weight) is a truncated form of its cellular homolog. The c-rel protein is found in the soluble cytoplasmic fraction of chicken lymphoid cells and is not associated in any stable way with other cellular proteins. Unlike the viral protein, p68c-rel is not detectably phosphorylated.
Simek
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Laboratory of Molecular Virology and Carcinogenesis, BRI-Basic Research Program, NCI-Frederick Cancer Research Facility, MD 21701.
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D000118:Acetylglucosaminidase; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002645:Chickens; D005347:Fibroblasts; D005814:Genes, Viral; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D020572:Oncogene Proteins v-rel; D010449:Peptide Mapping; D010766:Phosphorylation; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics*; D020569:Proto-Oncogene Proteins c-rel; D011519:Proto-Oncogenes; D001356:Reticuloendotheliosis virus / Q000235:genetics; D012191:Retroviridae Proteins / Q000032:analysis / Q000235:genetics*
D020572:Oncogene Proteins v-rel; D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; D012191:Retroviridae Proteins; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase