Cation-independent mannose 6-phosphate receptor contains covalently bound fatty acid.
The cation-independent mannose 6-phosphate receptor (215,000 daltons) was isolated from embryonic bovine tracheal cells and embryonic human skin fibroblasts labelled with [3H]palmitic acid. The tritium label was detected in the protein upon fluorographic analysis of SDS-polyacrylamide gels of the purified receptor. The label was not sensitive to hydroxylamine, methanolic KOH, or beta-mercaptoethanol, but labelled fatty acid was recovered from the protein by acidic methanolysis. Labelled receptor protein could not be isolated from cells grown in the presence of [3H]myristic acid. The results suggest the presence of amide-linked palmitic acid in the structure of the cation-independent mannose 6-phosphate receptor.
Westcott
K R
KR
Department of Biological Chemistry, UCLA School of Medicine 90024.
Rome
L H
LH
D000818:Animals; D002460:Cell Line; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008360:Mannosephosphates / Q000378:metabolism; D008970:Molecular Weight; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis / Q000378:metabolism*; D011485:Protein Binding; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism
D008360:Mannosephosphates; D009227:Myristic Acids; D010169:Palmitic Acids; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface; D019814:Myristic Acid; D019308:Palmitic Acid; D007930:Leucine