Use of a simian virus 40-based shuttle vector to analyze enhanced mutagenesis in mitomycin C-treated monkey cells.
When monkey cells were treated with mitomycin C 24 h before transfection with UV-irradiated pZ189 (a simian virus 40-based shuttle vector), there was a twofold increase in the frequency of mutations in the supF gene of the vector. These results suggest the existence of an enhancible mutagenesis pathway in mammalian cells. However, DNA sequence analysis of the SupF- mutants suggested no dramatic changes in the mechanisms of mutagenesis due to mitomycin C treatment of the cells.
Roilides
E
E
Section on Viruses and Cellular Biology, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Munson
P J
PJ
Levine
A S
AS
Dixon
K
K
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D005796:Genes; D005822:Genetic Vectors / Q000528:radiation effects; D007668:Kidney; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D012343:RNA, Transfer / Q000187:drug effects / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays
D008937:Mitomycins; D016685:Mitomycin; D012343:RNA, Transfer