title: Intracellular pH recovery and lactate efflux in mouse soleus muscles stimulated in vitro: the involvement of sodium/proton exchange and a lactate carrier.
abstract: The intracellular pH recovery after stimulation of mouse soleus muscles in vitro was studied by means of intracellular pH-sensitive microelectrodes. The lactate efflux and the total lactate content were measured by means of an enzymic method. During electrical stimulation for 2 min in a CO2/HCO3- -buffered Ringer's solution, pHi decreased by 0.5 units. The rate of pHi-recovery was independent of external bicarbonate, but dependent on the buffer concentration. The rate of intracellular pH recovery was reduced by the lactate transport inhibitors PCMBS and cinnamate, whereas the inhibitors of inorganic anion-exchange SITS and DIDS had no effect. The Na+/H+ exchange inhibitor amiloride reduced the rate of pHi recovery. The pHi recovery was faster than the lactate efflux, which could be accounted for by an Na+/H+ exchange. A number of inhibitor compounds were used in order to discriminate between the three possible lactate efflux pathways: the monocarboxylate carrier mechanism, the inorganic anion exchange, and the molecular (non-ionic) diffusion of lactic acid. The lactate efflux was partly inhibited by cinnamate, PCMBS and phloretin, but was unaffected by DIDS and tetrathionate. These experiments demonstrate the existence of a lactate carrier in mammalian skeletal muscles. The lactate carrier is responsible for more than half of the lactate efflux after muscle activity. Both the pHi recovery studies and the lactate efflux measurements showed that, under the given conditions, the inorganic anion-exchange mechanism is not essentially involved in the recovery processes after muscle activity.
authors.affiliation: Zoophysiological Lab. B., August Krogh Institute, Copenhagen, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Juel
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D006863:Hydrogen-Ion Concentration; D007474:Ion Exchange; D007773:Lactates / Q000378:metabolism*; D051379:Mice; D008839:Microelectrodes; D009132:Muscles / Q000378:metabolism / Q000502:physiology*; D011522:Protons; D012964:Sodium / Q000378:metabolism*
chemical_list: D007773:Lactates; D011522:Protons; D000584:Amiloride; D012964:Sodium