Initiation of lambda DNA replication reconstituted with purified lambda and Escherichia coli replication proteins.
Using highly purified bacteriophage lambda and E. coli replication proteins, we were able to reconstitute an in vitro system capable of replication ori lambda-containing plasmid DNA. The addition of a new E. coli factor, the grpE gene product, to this replication system reduced the level of dnaK protein required for efficient DNA synthesis by at least 10-fold, and also allowed the isolation of a stable DNA replication intermediate. Based on all available information, we propose a molecular mechanism for the action of the dnaK and grpE proteins during the prepriming reaction leading to lambda DNA synthesis.
Zylicz
M
M
Department of Molecular Biology, University of Gdansk, Kladki, Poland.
Ang
D
D
Liberek
K
K
Yamamoto
T
T
Georgopoulos
C
C
D001426:Bacterial Proteins / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000235:genetics*; D004261:DNA Replication; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli; D014764:Viral Proteins / Q000378:metabolism*
D001426:Bacterial Proteins; D004279:DNA, Viral; D014764:Viral Proteins; D004250:DNA Topoisomerases, Type II