Inhibition of mouse epidermal 12-lipoxygenase by 2,3,4-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinon e (AA861).
2,3,5-Trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone (AA861) strongly inhibited epidermal lipoxygenase activity which was determined by the formation of [14C]12-hydroxy-5,8,10,14-eicosatetraenoic acid by incubating [14C]arachidonic acid with cytosol fraction of epidermal homogenate of CD-1 mice. AA861 failed to inhibit epidermal cyclooxygenase activity. The present results indicate that AA861 is a potent inhibitor of 12-lipoxygenase in mouse epidermis (IC50 1.9 microM).
Nakadate
T
T
Yamamoto
S
S
Aizu
E
E
Kato
R
R
D000818:Animals; D001091:Arachidonate Lipoxygenases; D016227:Benzoquinones; D001792:Blood Platelets / Q000201:enzymology; D004817:Epidermis / Q000201:enzymology*; D005260:Female; D016859:Lipoxygenase Inhibitors; D051379:Mice; D008815:Mice, Inbred Strains; D011809:Quinones / Q000494:pharmacology*
D016227:Benzoquinones; D016859:Lipoxygenase Inhibitors; D011809:Quinones; C036837:2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone; D001091:Arachidonate Lipoxygenases