Fluorometric analysis of DNA in cell cultures.
A simple, reproducible fluorescent assay has been described which can be used confidently at microgram and submicrogram levels. The method is most suitable for cell cycle and cell growth analysis using [3H]thymidine as the radioactive tracer. A modified method is also described for preparing diaminobenzoic acid hydrochloride.
Janakidevi
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K
Department of Pathology, Albany Medical College, New York 12208.
Murray
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CD
Sell
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Held
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D062365:Aminobenzoates; D000818:Animals; D001011:Aorta / Q000032:analysis; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D006851:Hydrochloric Acid; D009131:Muscle, Smooth, Vascular / Q000032:analysis; D010472:Perchlorates; D013050:Spectrometry, Fluorescence; D013552:Swine
D062365:Aminobenzoates; D010472:Perchlorates; D004247:DNA; D006851:Hydrochloric Acid