Purification and properties of an enterotoxin from a coatless spore mutant of Clostridium perfringens type A.
A method is described for isolating an enterotoxin from a coatless spore mutant (8-6) of Clostridium perfringens type A. The characteristics of this enterotoxin only slightly resembled those of previously isolated enterotoxins of C. perfringens. The type A (8-6) enterotoxin was found to be composed of two subunits of Mr 18 000 with isoelectric points of 3.8 and 4.3. The LD50 for mice was 39 micrograms/kg with 0.10 micrograms corresponding to one erythemal unit. The type A (8-6) enterotoxin was inactivated by heating for 10 min at 60 degrees C. The amino acid composition data of type A (8-6) and delta toxins was similar, but type A (8-6) and type A enterotoxins showed less similarity. This lack of similarity between type A and type A (8-6) enterotoxins was confirmed by the failure of anti-sera to type A enterotoxin to neutralize the type A (8-6) enterotoxin, in both the mouse and erythemal tests.
Lindsay
J A
JA
Sleigh
R W
RW
Ghitgas
C
C
Davenport
J B
JB
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003016:Clostridium perfringens / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification* / Q000633:toxicity; D006358:Hot Temperature; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D009154:Mutation; D013056:Spectrophotometry, Ultraviolet; D013171:Spores, Bacterial / Q000032:analysis / Q000235:genetics
D000596:Amino Acids; D004768:Enterotoxins