Phorbol ester effects on neurotransmission: interaction with neurotransmitters and calcium in smooth muscle.
Stimulation of the phosphatidylinositol cycle by neurotransmitters generates diacylglycerol, an activator of protein kinase C, which may regulate some forms of neurotransmission. Phorbol esters, potent inflammatory and tumor-promoting compounds, also activate protein kinase C. We demonstrate potent and selective effects of phorbol esters on smooth muscle, indicating a role for protein kinase C in neurotransmission. In rat vas deferens and dog basilar artery, phorbol esters synergize with calcium to mimic the contractile effects of neurotransmitters that act through the phosphatidylinositol cycle. In guinea pig ileum and rat uterus, phorbol esters block contractions produced by these neurotransmitters.
Baraban
J M
JM
Gould
R J
RJ
Peroutka
S J
SJ
Snyder
S H
SH
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010095:Oxotremorine / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects*; D014599:Uterus / Q000187:drug effects; D014649:Vas Deferens / Q000187:drug effects; D014661:Vasoconstriction / Q000187:drug effects
D018377:Neurotransmitter Agents; D010703:Phorbol Esters; D010704:Phorbols; D010716:Phosphatidylinositols; D012701:Serotonin; D010095:Oxotremorine; D001285:Atropine; D006632:Histamine; D001920:Bradykinin; D002118:Calcium