Ca2+-dependent inactivation of acetylcholine receptors by an endogenous transglutaminase.
The nicotinic acetylcholine receptor (nAChR) from Torpedo californica and T. marmorata electric tissue polymerises irreversibly when DTE and Ca2+ are added to receptor-rich membranes. The polymerisation is time-dependent and complete within 3 h at 30 degrees C. It can be completely prevented by EGTA or the transglutaminase inhibitor cystamine. Transglutaminase activity can also be monitored with the exogenous substrates [3H]putrescine and dimethylcasein. This assay can also be inhibited by EGTA or cystamine.
Hucho
F
F
Bandini
G
G
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000502:physiology*; D004229:Dithiothreitol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D011108:Polymers / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014101:Torpedo; D011503:Transglutaminases / Q000032:analysis / Q000502:physiology*
D011108:Polymers; D011978:Receptors, Nicotinic; D004492:Edetic Acid; D011503:Transglutaminases; D000109:Acetylcholine; D002118:Calcium; D004229:Dithiothreitol