Prostanoids and adrenaline release: a study of [3H]adrenaline efflux from the rabbit isolated, perfused, adrenal gland.
[3H]Adrenaline was incorporated in an isolated perfused preparation of the rabbit adrenal gland and the effects of indomethacin, PGE2 and PGI2 on its release were investigated. Efflux of [3H]adrenaline was elicited by electrical stimulation of the splanchnic nerve (60 s at 5 Hz). Indomethacin (3 and 30 microM) had no effect on stimulation-induced efflux. PGE2 (30, 90 and 300 nM) reduced the efflux; with 90 nM PGE2 the inhibition amounted to approximately 30%. PGI2, in concentrations from 90 to 600 nM, was without effect. These findings indicate that release of [3H]adrenaline from the rabbit adrenal gland is not subject to modulation by endogenous adrenal prostaglandins; however, PGE2 may play a role in some pathological situations.
Collett
A R
AR
Dusting
G J
GJ
Story
D F
DF
Purchase
M
M
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D015232:Dinoprostone; D004558:Electric Stimulation; D004837:Epinephrine / Q000378:metabolism*; D011464:Epoprostenol / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology; D011817:Rabbits; D013153:Splanchnic Nerves / Q000502:physiology
D011453:Prostaglandins; D011458:Prostaglandins E; D011464:Epoprostenol; D015232:Dinoprostone; D007213:Indomethacin; D004837:Epinephrine