title: Rapid and precise micro-methods for quantitating active components in commercial bone scintigraphy kits--II. Diphosphonates.
abstract: Two parallel, independent micro-methods for diphosphonate determination are presented. These procedures are based on the interference of diphosphonate with the formation of cation-morin (3,5,7,2',4'-pentahydroxyflavone) complexes. In the spectrophotometric method, a change of the absorbance of thorium-morin complex is used as a measure of diphosphonate concentration. In the fluorimetric method, a decrease of the fluorescence of aluminium-morin complex is used. Both methods: (1) quantitate methylene-diphosphonate in the range of 5-40 x 10(-9) M with a coefficient of variation of less than 1%; (2) are specific and interference-free in commercial kits control; (3) are operative with other diphosphonates; and (4) are adaptable to the analysis of chromatographic eluents.
authors.affiliation: Catholic University of Louvain, School of Pharmacy, Department of Medicinal Chemistry, Brussels, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Gallez
authors.forename: P
authors.initials: P
authors.lastname: Osinski
authors.forename: J
authors.initials: J
authors.lastname: Adline
authors.forename: P
authors.initials: P
authors.lastname: Dumont
mesh_terms: D001842:Bone and Bones / Q000000981:diagnostic imaging*; D004164:Diphosphonates / Q000032:analysis*; D006801:Humans; D008722:Methods; D011877:Radionuclide Imaging; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D013669:Technetium Tc 99m Medronate
chemical_list: D004164:Diphosphonates; D011933:Reagent Kits, Diagnostic; D013669:Technetium Tc 99m Medronate