Antigenic variation in alkaline deoxyribonuclease induced by three different strains of Epstein-Barr virus.
To determine whether biological and/or biochemical variants exist between strains of Epstein-Barr virus (EBV), we superinfected Raji cells with the nontransforming lytic strain of EBV (HR-1), and two isolates that both transform B-lymphocytes and superinfect Raji cells, B95-8, and NPC-EBV. The superinfected cells were assayed for EBV specific DNase. A new electrophoretic form of DNase was observed in cells superinfected with B95-8 EBV as compared to the enzymes induced by the HR-1 and NPC-EBV isolates. There were antigenic differences in the DNase induced by the EBV strains. Since antibody to EBV DNase is a marker for nasopharyngeal carcinoma (NPC), these data may have implications for EBV-associated disease.
Williams
M V
MV
Department of Medical Microbiology and Immunology, Ohio State University, Columbus 43210.
Boak
A
A
Glaser
R
R
D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D003851:Deoxyribonucleases / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004854:Herpesvirus 4, Human / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests
D000956:Antigens, Viral; D003851:Deoxyribonucleases