Distribution of [125I]omega-conotoxin GVIA and [3H]isradipine binding sites in the central nervous system of rats of different ages.
Potential age-related changes in L- and N-type voltage-sensitive calcium channels (L- and N-VSCCs) were assessed by the in vitro binding of [3H]isradipine ([3H]ISR, 150 pM) and [125]omega-conotoxin GVIA ([125I]omega-CT, 4 pM) to membranes prepared from discrete central nervous system regions of 0.5-, 2-, and 18-month-old rats. The rank orders of [3H]ISR and [125I]omega-CT binding, although differing, indicated that the highest binding was in neocortex, corpus striatum, and hippocampus; radioligand binding was generally not affected by the variable of age. These results suggest that the nonidentical [3H]ISR and [125I]omega-CT binding sites are concentrated in those regions characterized by high densities of synaptic connections, and that these sites, as presumed components of L- and N-VSCCs, are relatively stable during the aging process.
Dooley
D J
DJ
Department of Pharmacology, Gödecke Research Institute, Freiburg, F.R.G.
Lickert
M
M
Lupp
A
A
Osswald
H
H
D000375:Aging / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D001665:Binding Sites; D015220:Calcium Channels / Q000502:physiology*; D002490:Central Nervous System / Q000378:metabolism*; D004594:Electrophysiology; D007457:Iodine Radioisotopes; D017275:Isradipine; D008297:Male; D008978:Mollusk Venoms / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D020866:omega-Conotoxin GVIA
D015220:Calcium Channels; D007457:Iodine Radioisotopes; D008978:Mollusk Venoms; D011725:Pyridines; D014316:Tritium; D020866:omega-Conotoxin GVIA; D017275:Isradipine