Myo-inositol(1,4,5)trisphosphorothioate binds to specific [3H]inositol(1,4,5)trisphosphate sites in rat cerebellum and is resistant to 5-phosphatase.
D-Myo-inositol(1,4,5)trisphosphorothioate, a synthetic analogue of inositol(1,4,5)trisphosphate was shown to bind with a relatively high affinity to specific sites on rat cerebellar membranes labelled with [3H]inositol(1,4,5)trisphosphate. Use of this binding assay has also established that unlike the trisphosphate, the trisphosphorothioate is completely resistant to a specific 5-phosphatase prepared from human erythrocytes. The ability of this novel analogue to release intracellular Ca2+ has already been reported and it offers considerable potential in the investigation of phosphoinositide-linked receptors.
Willcocks
A L
AL
Department of Pharmacology and Therapeutics, University of Leicester, U.K.
Potter
B V
BV
Cooke
A M
AM
Nahorski
S R
SR
D000818:Animals; D001665:Binding Sites; D002531:Cerebellum / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D006801:Humans; D007294:Inositol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000031:analogs & derivatives; D009946:Organothiophosphorus Compounds / Q000138:chemical synthesis / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D013237:Stereoisomerism
D009946:Organothiophosphorus Compounds; C054737:inositol 1,4,5-triphosphorothioate; D007294:Inositol; D015544:Inositol 1,4,5-Trisphosphate; D010744:Phosphoric Monoester Hydrolases