Rapid identification of penicillinase-producing Neisseria gonorrhoeae by detection of beta-lactamase in urethral exudates.
The production of fluorescent end-products with ampicillin as substrate can be used to detect beta-lactamase activity in biological fluids. A fluorescent spot test was evaluated as a rapid method of detecting beta-lactamase in the urethral exudates of men infected with Neisseria gonorrhoeae in Thailand. Among 208 men with culture-proven gonococcal urethritis, the fluorescent spot test was positive in 92 of 101 men from whom penicillinase-producing N gonorrhoeae (PPNG) were isolated and in 4 of 107 men from whom non-PPNG were isolated. The fluorescent spot test is a rapid, inexpensive, and sensitive method to detect PPNG and may be valuable in determining appropriate treatment for patients with gonococcal urethritis.
Taylor
D N
DN
Chen
K C
KC
Panikabutra
K
K
Wongba
C
C
Chitwarakern
A
A
Echeverria
P
P
Holmes
K K
KK
D005122:Exudates and Transudates / Q000382:microbiology*; D005453:Fluorescence; D006069:Gonorrhea / Q000382:microbiology*; D006801:Humans; D008297:Male; D008722:Methods; D009344:Neisseria gonorrhoeae / Q000201:enzymology* / Q000302:isolation & purification; D010405:Penicillinase / Q000096:biosynthesis*; D014521:Urethra / Q000382:microbiology*; D001618:beta-Lactamases / Q000032:analysis*
D010405:Penicillinase; D001618:beta-Lactamases