Similar DNA restriction endonuclease profiles in strains of Legionella pneumophila from different serogroups.
DNA of strains of Legionella pneumophila serogroups 1, 3, 4, and 6, isolated from patients and environmental sources, was examined by restriction endonuclease analysis (REA). Major differences in profiles enabled subtyping in many strains with the same serogroup antigen. However, a cluster of L. pneumophila strains, originating from all the examined serogroups, had similar restriction endonuclease profiles, sometimes with minor differences. This suggests that the genetic similarity between strains of L. pneumophila of different serogroups is sometimes closer than in strains with the same serogroup antigen. Seven environmental sources harbored two L. pneumophila strains with various serogroup antigens; six sources had similar restriction endonuclease profiles. The resolution of small differences in profiles is hampered in REA by the great magnitude of DNA fragments; even upon extensive analysis, these differences are not always readily visualized. Double digestions with the restriction enzymes HpaI and HpaII showed the best results and sometimes revealed differences not evident by digestions with a single endonuclease. REA has a great capacity for accurate epidemiological typing of L. pneumophila, in addition to classical serogrouping; it appeared that the results of the two techniques do not necessarily correlate. On the other hand, it should be stressed that small differences in profiles are not easily detected by REA.
van Ketel
R J
RJ
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006761:Hospitals; D006801:Humans; D007875:Legionella / Q000145:classification / Q000235:genetics*; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology*; D000091402:Prohibitins; D012703:Serotyping; D014871:Water Microbiology; D014881:Water Supply
D004269:DNA, Bacterial; C000717331:PHB2 protein, human; D000091402:Prohibitins; D004262:DNA Restriction Enzymes