[Primary structure of the OSCP protein that confers sensitivity to oligomycin on the mitochondrial H+-ATPase complex. I. Tryptic and cyanogen bromide peptides].
Trypsin and cyanogen bromide were used for cleavage of the OSCP preparations. The peptide mixtures thus formed were separated into individual components by a combination of various chromatographic procedures: gel filtration, ion exchange and paper chromatography, as well as reversed-phase HPLC. As a result, 31 tryptic peptides and 9 out of 10 possible cyanogen bromide peptides were isolated. Determination of the amino acid sequences of these peptide allowed the alignment of cyanogen bromide fragments in the polypeptide chain that shed light on the "architecture" of the protein molecule as a whole. It also afforded the overlappings for tryptic peptides, 16 in the N-terminal and 8 in the C-terminal portions of the molecule.
Grinkevich
V A
VA
Trubetskaia
O E
OE
Chertova
E N
EN
Murav'eva
T I
TI
Aldanova
N A
NA
D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D002417:Cattle; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D003488:Cyanogen Bromide; D008297:Male; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D008929:Mitochondria, Heart / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases; D009840:Oligomycins / Q000494:pharmacology*; D010446:Peptide Fragments / Q000032:analysis*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D014357:Trypsin
D002352:Carrier Proteins; D008565:Membrane Proteins; D009840:Oligomycins; D010446:Peptide Fragments; D014357:Trypsin; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; C020439:oligomycin sensitivity-conferring protein; D003488:Cyanogen Bromide
Pervichnaia struktura OSCP--belka, pridaiushchego mitokhondrial'nomu H+-ATP-aznomu kompleksu chuvstvitel'nost' k oligoitsinu. I. Tripticheskie i bromtsianovye peptidy.