title: Inconsistent sodium current records derived on Ranvier nodes with a commercially available potential clamp device according to Nonner.
abstract: We tried to reproduce some basic implications of the Hodgkin-Huxley-Frankenhaeuser formalism by measuring sodium currents in single myelinated nerve fibres with a commercially available version of the potential clamp device according to Nonner. The following contradictory observations were made: 1. The potential dependence of the time to peak sodium currents showed a discontinuity around the sodium equilibrium potential. 2. Defining the sodium permeability PNa by the constant field equation and fitting the peak PNa-voltage relation by a sigmoid function we obtained unbelievable high values of PNa at rest. 3. Testing PNa as calculated by the constant field equation by so-called "sodium tail current" experiments we obtained instantaneous changes of PNa. Summing up, neither the kinetics of sodium currents nor the constant field concept as tested with the equipment used seem to agree satisfactorily with the standard data of sodium currents in Ranvier nodes.
authors.affiliation: Institute of Physiology, University of Kiel, FRG.
authors.forename: E
authors.initials: E
authors.lastname: Koppenhöfer
authors.forename: K H
authors.initials: KH
authors.lastname: Bohuslavizki
mesh_terms: D000818:Animals; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D007700:Kinetics; D008564:Membrane Potentials; D009413:Nerve Fibers, Myelinated / Q000502:physiology*; D011901:Ranvier's Nodes / Q000502:physiology*; D012584:Sciatic Nerve / Q000502:physiology; D015222:Sodium Channels / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: D015222:Sodium Channels