Suppression of neutrophil superoxide production by conventional peritoneal dialysis solution.
The pH of conventional peritoneal dialysis solution is normally in the range of 5.0 to 5.5, because acid has been added during the manufacturing process to prevent caramelization of dextrose during sterilization. We studied the effects of normalizing the pH of conventional peritoneal dialysis solution on superoxide production by normal human neutrophils. At a pH of 6.0, superoxide generation was 4.07 +/- 2.56 (SD) nanomoles per million cells. With normalization of pH to 7.4, superoxide production was 19.3 +/- 7.3 (p less than 0.001). The results suggest that the unphysiologic acidity of conventional peritoneal dialysis solution has deleterious consequences on neutrophil superoxide formation.
Ing
B L
BL
Department of Medicine, Veterans Administration Hospital, Hines, Illinois.
Gupta
D K
DK
Nawab
Z M
ZM
Zhou
F Q
FQ
Rahman
M A
MA
Daugirdas
J T
JT
D000293:Adolescent; D000328:Adult; D015314:Dialysis Solutions / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010530:Peritoneal Dialysis; D013481:Superoxides / Q000096:biosynthesis*
D015314:Dialysis Solutions; D013481:Superoxides