title: Spontaneous insertion of an IS2 element into the promoter region of the lac operon.
abstract: A spontaneous mutation in pUC18 has revealed the insertion of a chromosomal insertion sequence (IS)2 element into the promoter region of the lac operon. The IS2 insertion site, at the pentanucleotide sequence TCGAG, is unlike previously described junctional sequences.
authors.affiliation: Department of Medicine, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111.
authors.forename: P
authors.initials: P
authors.lastname: De Togni
authors.forename: E S
authors.initials: ES
authors.lastname: Fox
authors.forename: S
authors.initials: S
authors.lastname: Morrissey
authors.forename: B M
authors.initials: BM
authors.lastname: Babior
authors.forename: S B
authors.initials: SB
authors.lastname: Levy
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014169:Transformation, Bacterial
chemical_list: D004251:DNA Transposable Elements