Cis-acting sequences responsive to the rev gene product of the human immunodeficiency virus.
Expression of high levels of the structural proteins of the human immunodeficiency virus type 1 (HIV-1) requires the presence of two regulatory genes, the trans-activator (tat), and the regulator of virion protein expression (rev. previously called art or trs). The experiments described here show that expression of virion proteins is dependent upon a small region located in the envelope gene called the cis-acting antirepression sequence (CAR). The CAR region of the envelope sequence is both necessary and sufficient for rev-dependent capsid protein expression. The experiments also show that a defect in either rev or CAR results in a dramatic decrease in the accumulation of the genomic and envelope mRNAs and an overproduction of more extensively spliced viral mRNA species.
Dayton
A I
AI
Department of Pathology, Harvard Medical School, Dana-Farber Cancer Institute, Boston, MA 02115.
Terwilliger
E F
EF
Potz
J
J
Kowalski
M
M
Sodroski
J G
JG
Haseltine
W A
WA
D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis; D002478:Cells, Cultured; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D015694:Gene Products, rev; D005809:Genes, Regulator; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication; D054321:rev Gene Products, Human Immunodeficiency Virus
D004251:DNA Transposable Elements; D015694:Gene Products, rev; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus