title: The mechanism of enzymically induced chemiluminescence reactions of lucigenin.
abstract: Lucigenin (Bis-N-methylacrylium nitrate) reacts with H2O2 to produce ground state and excited state monomer. The excited state monomer decays with the emission of light. When lucigenin is added to reaction mixtures containing vitamin-K-dependent carboxylase, light emission is produced which is inversely proportional to enzyme concentration and co-inhibited with inhibitors of vitamin-K-dependent carboxylase.
authors.affiliation: Department of Chemistry, McNeese State University, Lake Charles, LA 70609-0455.
authors.forename: U S
authors.initials: US
authors.lastname: Ramelow
mesh_terms: D000166:Acridines; D000818:Animals; D019735:Carbon-Carbon Ligases; D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; D007202:Indicators and Reagents; D007700:Kinetics; D007834:Lasers; D008025:Ligases / Q000032:analysis* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008163:Luminescent Measurements; D008297:Male; D010782:Photolysis; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000166:Acridines; D005609:Free Radicals; D006878:Hydroxides; D007202:Indicators and Reagents; C033472:10,10'-dimethyl-9,9'-biacridinium; D017665:Hydroxyl Radical; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase