Protein immunoassay based on agglutination of antibody-coated ferric oxide particles.
A novel immunoassay is described based on the agglutination by antigens of antibody-coated ferric oxide (Fe2O3) particles. The procedure for attachment of antibodies to the ferric oxide particles is simple and fast. The assay was used for identification and quantification of gamma-globulin in human serum and in chromatographic fractions as well as of gp70 (an envelope protein from feline leukemia virus) in the culture medium and for identification of the thyroid-stimulating hormone (TSH).
Zelikman
I
I
Institute of Biochemistry, University of Uppsala, Sweden.
Hjertén
S
S
D000372:Agglutination Tests / Q000379:methods; D002498:Centrifugation; D002845:Chromatography; D003470:Culture Media; D005290:Ferric Compounds; D006112:Gravitation; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis; D005256:Leukemia Virus, Feline; D008853:Microscopy; D011309:Preservation, Biological; D011506:Proteins / Q000032:analysis*; D012191:Retroviridae Proteins / Q000032:analysis; D013972:Thyrotropin / Q000032:analysis
D003470:Culture Media; D005290:Ferric Compounds; D007074:Immunoglobulin G; D011506:Proteins; D012191:Retroviridae Proteins; C000499:ferric oxide; D013972:Thyrotropin