[The effect of spermine on transport of Ca2+ ions in brain mitochondria].
The effect of spermine (50-400 microM) on the Ca-transporting system of brain mitochondria was studied. In a medium containing Mg2+ and ATP, spermine facilitates the accumulation of Ca2+ by decreasing Km of the uniporter. Spermine inhibits Na-stimulated Ca2+ efflux; this effect is dependent on the ionic strength of the medium--it is decreased when KCl concentration is increased from 20 to 120 mM. Spermine (200 microM) decreases (by 50%) the steady state concentration of Ca2+ maintained by mitochondria. The importance of spermine as a regulator of Ca2+-transport in brain mitochondria is discussed.
Karadzhov
Iu S
IuS
Kudzina
L Iu
LIu
Zinchenko
V P
VP
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects; D002413:Cations, Divalent / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D009994:Osmolar Concentration; D011817:Rabbits; D013096:Spermine / Q000494:pharmacology*
D015220:Calcium Channels; D002413:Cations, Divalent; D013096:Spermine; D002118:Calcium
Vliianie spermina na transport ionov Ca2+ v mitokhondriiakh mozga.