Inhibition of growth hormone-induced lipolysis by 3',5'-guanosine monophosphate in chicken adipose tissue in vitro.
The influence of cyclic 3',5'-guanosine monophosphate (cGMP) on the lipolytic and antilipolytic (inhibition of glucagon-stimulated lipolysis) responses to GH (1 microgram/ml) was examined in chicken adipose tissue in vitro. Both 8-bromo-cGMP (0.1 mM) and sodium nitroprusside (1 mM) (a guanyl cyclase stimulator) completely inhibited the lipolytic effect of GH. A cGMP-lowering agent, LY83583 (10 microM), reversed the inhibitory effect of sodium nitroprusside on GH-stimulated lipolysis. Furthermore, the suppressive effects of insulin (100 ng/ml), insulin-like growth factor I (IGF-I) (100 ng/ml), or insulin-like growth factor II (IGF-II/MSA) (100 ng/ml), but not somatostatin (1 ng/ml), on GH-stimulated lipolysis were prevented by LY83583 addition. Neither 8-bromo-cGMP, sodium nitroprusside, nor LY83583 altered GH-induced inhibition of glucagon (1 ng/ml)-stimulated lipolysis. It is proposed that cGMP may mediate inhibitory control of GH-stimulated lipolysis by insulin, IGF-I, and IGF-II in chicken adipose tissue.
Campbell
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Program in Nutrition, State University of New Jersey, New Brunswick 08903.
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D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002645:Chickens; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D005934:Glucagon; D013006:Growth Hormone / Q000037:antagonists & inhibitors*; D007328:Insulin / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D013002:Somatomedins / Q000494:pharmacology
D000634:Aminoquinolines; D007328:Insulin; D011994:Recombinant Proteins; D013002:Somatomedins; D009599:Nitroprusside; C016276:8-bromocyclic GMP; D013006:Growth Hormone; D005934:Glucagon; C041715:6-anilino-5,8-quinolinedione; D006152:Cyclic GMP