Nitrogen regulation in Salmonella typhimurium. Identification of an ntrC protein-binding site and definition of a consensus binding sequence.
We have investigated the DNA-binding ability of a nitrogen regulatory protein, the product of the ntrC gene, to several nitrogen-regulated promoters in Salmonella typhimurium. The ntrC protein is able to bind to the regulatory region (dhuA) of an operon coding for genes involved in the active transport of histidine, but not to another transport-related regulatory region, argTr. It bound to two different sites within the regulatory region of glnA (glutamine synthetase) and to one site in the regulatory region for the ntrBC operon. A consensus sequence has been derived from these four binding sites. The binding sequence displays dyad symmetry, as expected for the dimeric ntrC protein. The relationship of the binding sites to regulation of transcription initiation and termination, and to published homologies within the sequences of regulatory sites for nif genes is discussed.
Ferro-Luzzi Ames
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Nikaido
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D026905:Amino Acid Transport Systems; D001120:Arginine / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001665:Binding Sites; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005809:Genes, Regulator; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D006639:Histidine / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics; D009584:Nitrogen / Q000378:metabolism*; D009876:Operon; D012486:Salmonella typhimurium / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D026905:Amino Acid Transport Systems; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D026901:Membrane Transport Proteins; D014157:Transcription Factors; D006639:Histidine; D001120:Arginine; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen