Strain differences in the response of normal murine spleen cells to interleukin-2 (IL-2).
Susceptibility of normal spleen cells from various murine strains to IL-2 was assessed. The strains could be classified as highly susceptible (C57BL/6Kh, B6.PL(74NS), AKR.B6, and AKR.M) and moderately susceptible (C3H.B10 and C3H.A). These differences were demonstrable when cells were grown in the presence of relatively high concentrations of crude or highly purified IL-2 preparations, but were undetectable in low concentrations. The susceptibility appears to be genetically determined by multiple dominant or codominant genes that most likely control quantitative and/or qualitative differences in the composition of T-cell population residing in the normal spleen. From a practical point of view, the results suggest a need for testing experimental mice for their susceptibility to IL-2 prior to designing specific experiments.
Dowjat
W K
WK
Quackenbush
L J
LJ
Zaleski
M B
MB
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis; D002478:Cells, Cultured; D002999:Clone Cells / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D007376:Interleukin-2 / Q000008:administration & dosage / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000276:immunology; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D007376:Interleukin-2; D018800:Thy-1 Antigens