title: Plasminogen activator inhibitor 2. Isolation and characterization of the promoter region of the gene.
abstract: We have isolated the promoter region and part of the coding region of the human plasminogen activator inhibitor 2 gene. The first two exons (65 and 177 base pairs, respectively) are separated by a 3.6 kilo base pair intron. More than 2 kilo base pair of the promoter region was sequenced and analyzed. The promoter region is characterized by: a) a TATAAA box located 31 base pairs upstream of the transcription initiation site; b) a 123 base pair stretch (-598 to -720) that is homologous (greater than 90% identity) to the inverted sequence of -1268 to -1380; c) a 24 base pair inverted repeat at -276 to -299 and three direct repeats; and d) several sequences that are homologous (up to 1 mismatch) to the cAMP responsive element or to binding sites for the transcription factors AP1 and AP2.
authors.affiliation: Department of Medicine, University Hospital, Lausanne, Switzerland.
authors.forename: E K
authors.initials: EK
authors.lastname: Kruithof
authors.forename: E
authors.initials: E
authors.lastname: Cousin
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D005091:Exons; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors; D015849:Plasminogen Inactivators; D011401:Promoter Regions, Genetic
chemical_list: D006023:Glycoproteins; D015849:Plasminogen Inactivators; D004247:DNA; D004262:DNA Restriction Enzymes; D010960:Plasminogen Activators