Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype.
In 1983, we isolated a porcine rotavirus (strain YM) that was prevalent in several regions of Mexico, as judged by the frequency of its characteristic electropherotype. By a focus reduction neutralization test, rotavirus YM was clearly distinguished from prototype rotavirus strains belonging to serotypes 1 (Wa), 2 (S2), 3 (SA11), 4 (ST3), 5 (OSU), and 6 (NCDV). Minor, one-way cross-neutralization (1 to 5%) was observed when antisera to the various rotavirus strains were incubated with rotavirus YM. In addition, the YM virus was not neutralized by neutralizing monoclonal antibodies with specificity to serotypes 1, 2, 3, and 5. The subgroup of the virus was determined to be I by enzyme-linked immunosorbent assay. To characterize the serotype-specific glycoprotein of the virus at the molecular level, we cloned and sequenced the gene coding for VP7. Comparison of the deduced amino acid sequence with reported homologous sequences from human and animal rotavirus strains belonging to six different serotypes further supported the distinct immunological identity of the YM VP7 protein.
Ruiz
A M
AM
Departamento de Biologia Molecular, Centro de Investigaciones sobre Ingenieria Genetica y Biotecnologia, Cuernavaca, Morelos, Mexico.
López
I V
IV
López
S
S
Espejo
R T
RT
Arias
C F
CF
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D007106:Immune Sera; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012401:Rotavirus / Q000145:classification* / Q000302:isolation & purification; D012703:Serotyping; D013552:Swine / Q000382:microbiology*; D014764:Viral Proteins / Q000235:genetics
D000956:Antigens, Viral; D006023:Glycoproteins; D007106:Immune Sera; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins