Identification of the protein encoded by rodC, a cell division gene from Bacillus subtilis.
The rodC1 mutation of Bacillus subtilis is a temperature-sensitive marker which affects the orientation of the plane of cell division. We have cloned the rodC gene and have localized the site of the rodC1 lesion. To identify the rodC gene product, we have subjected several plasmid clones containing B. subtilis chromosomal DNA from the rodC region to maxicell analysis in Escherichia coli. A 68 kiloDalton protein has been identified as the rodC gene product. This is the initial cloning of a cell division gene and the identification of its product from B. subtilis. The rodC gene has also been implicated as being directly associated with the synthesis of glycerol teichoic acid.
Honeyman
A L
AL
Department of Microbiology, University of Kansas, Lawrence 66045.
Stewart
G C
GC
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002455:Cell Division; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial