[The role of vitamin K in the interaction of 1,25-dihydroxyvitamin D3 receptors with DNA].
Vitamin K deficiency in rats caused a rise of in vivo occupied 1,25(OH)2D3 receptor level in chromatin of the intestinal mucosa and a marked (2-2.5-fold) increase of intestinal cytosolic 1,25(OH)2D3-receptor complex binding with heterologous DNA, whereas maximum binding capacity and equilibrium dissociation constant of cytosolic 1,25 (OH)2D3 receptors did not change. Preincubation of renal and intestinal cytosol of vitamin K-deficient rats with microsomal vitamin K-dependent gamma-carboxylating system reduced sharply 1,25(OH)2D3-receptor complex binding with DNA. In rats treated by vitamin K antagonist along with a low calcium diet, no dramatic decrease of occupied 1,25(OH)2D3 receptors occurred after the animals were maintained with a high calcium diet. No such effect was observed in vitamin K-replete rats. The data demonstrate vitamin K-dependent Ca-sensitive qualitative modification of 1,25(OH)2D3 receptor dropping its binding performance to DNA.
Sergeev
I N
IN
Spirichev
V B
VB
D015110:4-Hydroxycoumarins / Q000494:pharmacology; D000818:Animals; D002117:Calcitriol / Q000008:administration & dosage / Q000378:metabolism*; D002136:Calcium, Dietary / Q000008:administration & dosage; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D004247:DNA / Q000031:analogs & derivatives / Q000378:metabolism*; D004347:Drug Interactions; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D014812:Vitamin K / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014813:Vitamin K Deficiency / Q000378:metabolism
D015110:4-Hydroxycoumarins; D002136:Calcium, Dietary; C033190:DNA-cellulose; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D014812:Vitamin K; D002482:Cellulose; D004247:DNA; D002117:Calcitriol
Rol' vitamina K vo vzaimodeĭstvii retseptorov 1,25-digidroksivitamina D3 s DNK.