title: [Use of growth hormone genes for the diagnosis of the disease of dwarfism].
abstract: The pattern of BamHI fragments of DNA from three children suggested to suffer the isolated growth hormone deficiency type. IA was not different from normal pattern registered in blot hybridization with [32P]cDNA of the growth hormone gene. The data permits one to exclude the above mentioned disease that is characterized by the deletion of HGH-N gene. The analogous DNA restriction analysis using HindIII restriction endonuclease has shown, that neither the sick children, nor their parents carry the deletion in heterozygotic state. The study of normal polymorphism of the restriction fragments length has shown that as for as the frequency of polymorphic MspI restriction endonuclease sites A and B in the growth hormone gene cluster (0.67 and 0.75 respectively) is concerned the Russian population in Moscow is closer to Mediterranean one than to North-european.
authors.forename: V n
authors.initials: Vn
authors.lastname: Kalinin
authors.forename: G I
authors.initials: GI
authors.lastname: Shipitsyna
authors.forename: M L
authors.initials: ML
authors.lastname: Kikodze
authors.forename: P M
authors.initials: PM
authors.lastname: Rubtsov
authors.forename: P S
authors.initials: PS
authors.lastname: Sverdlova
mesh_terms: D004262:DNA Restriction Enzymes; D004392:Dwarfism / Q000175:diagnosis* / Q000235:genetics; D013006:Growth Hormone / Q000172:deficiency / Q000235:genetics*; D006801:Humans; D005810:Multigene Family
chemical_list: D013006:Growth Hormone; D004262:DNA Restriction Enzymes
vernacular_title: Ispol'zovanie genov gormona rosta dlia diagnostiki zabolevaniia karlikovost'iu.