Evidence of gene amplification in tunicamycin-resistant Chinese hamster ovary cells.
Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast transferase gene, ALG7. The data presented suggest moderate amplification of the N-acetylglucosaminyl-1-phosphate transferase gene occurred in these cells, consistent with the previously observed chromosomal translocations and increased enzymatic activity. This is the first example of gene amplification as a mechanism for aberrations in N-linked glycosylation.
Scocca
J R
JR
Department of Biochemistry, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland.
Hartog
K O
KO
Krag
S S
SS
D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D015342:DNA Probes; D004351:Drug Resistance; D005260:Female; D005784:Gene Amplification; D009693:Nucleic Acid Hybridization; D010053:Ovary; D010770:Phosphotransferases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017855:Transferases (Other Substituted Phosphate Groups); D014415:Tunicamycin / Q000494:pharmacology*
D015342:DNA Probes; D014415:Tunicamycin; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C023222:UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase