Changes in alpha-lactalbumin, total lactose, UDP-galactose hydrolase and other factors in tammar wallaby (Macropus eugenii) milk during lactation.
alpha-Lactalbumin was isolated from milk of M. eugenii and its concentration in milk samples taken at various times during lactation (0-40 weeks post partum) was determined by single radial immunodiffusion using rabbit antiserum to the purified protein. The alpha-lactalbumin concentration remained almost constant throughout lactation even though the concentration of total lactose (free lactose plus lactose contained in oligosaccharides) fell to zero after 34 weeks post partum. This fall in lactose was accompanied by a rise in the free galactose and glucose concentrations and marked increases in UDP-galactose hydrolase, nucleotide pyrophosphatase, alkaline phosphatase and acid beta-galactosidase activities. It is suggested that the in vitro hydrolysis of UDP-galactose was due to nucleotide pyrophosphatase and that this enzyme may also play a role in vivo late in lactation by making UDP-galactose unavailable for the synthesis of lactose. Alternatively, lactose and lactose-containing oligosaccharides might be degraded by the acid beta-galactosidase during or after secretion.
Messer
M
M
Department of Biochemistry, University of Sydney, N.S.W.
Elliott
C
C
D000818:Animals; D050260:Carbohydrate Metabolism; D005260:Female; D005695:Galactosephosphates / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D007768:Lactalbumin / Q000378:metabolism; D007774:Lactation / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D008394:Marsupialia / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011247:Pregnancy; D014531:Uridine Diphosphate Galactose / Q000378:metabolism
D005695:Galactosephosphates; C029973:galactose-1-phosphate; D014531:Uridine Diphosphate Galactose; D007768:Lactalbumin; D005964:Glucosyltransferases; C059055:N-acetylglucosaminyldiphosphoundecaprenol glucosyltransferase; D010727:Phosphoric Diester Hydrolases; C060071:UDP-galactose hydrolase; D007785:Lactose