Interaction of estramustine phosphate with microtubule-associated proteins.
We have reported [(1984) Cancer Res., in press] that estramustine phosphate inhibits microtubule assembly and disassembled preformed microtubules. We now present evidence that estramustine phosphate inhibits microtubule assembly by binding to the microtubule-associated proteins. We have found that: additional microtubule-associated proteins relieved the inhibition of assembly by estramustine phosphate; 3H-labelled estramustine phosphate bound predominantly to the microtubule-associated proteins; and the content of the microtubule-associated proteins was reduced in taxol reversed estramustine phosphate-inhibited microtubules.
Wallin
M
M
Deinum
J
J
Fridén
B
B
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004961:Estramustine / Q000378:metabolism* / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000502:physiology*; D009588:Nitrogen Mustard Compounds / Q000378:metabolism*; D017239:Paclitaxel; D014404:Tubulin / Q000378:metabolism
D000470:Alkaloids; D008869:Microtubule-Associated Proteins; D009588:Nitrogen Mustard Compounds; D014404:Tubulin; D004961:Estramustine; D006493:Heparin; D017239:Paclitaxel