title: Lesions of entorhinal cortex produce a calpain-mediated degradation of brain spectrin in dentate gyrus. I. Biochemical studies.
abstract: Lesions of the rat entorhinal cortex cause extensive synaptic restructuring and perturbation of calcium regulation in the dentate gyrus of hippocampus. Calpain is a calcium-activated protease which has been implicated in degenerative phenomena in muscles and in peripheral nerves. In addition, calpain degrades several major structural neuronal proteins and has been proposed to play a critical role in the morphological changes observed following deafferentation. In this report we present evidence that lesions of the entorhinal cortex produce a marked increase in the breakdown of brain spectrin, a substrate for calpain, in the dentate gyrus. Two lines of evidence indicate that this effect is due to calpain activation: (i) the spectrin breakdown products observed following the lesion are indistinguishable from calpain-generated spectrin fragments in vitro; and (ii) their appearance can be reduced by prior intraventricular in fusion of leupeptin, a calpain inhibitor. Levels of spectrin breakdown products are increased as early as 4 h post-lesion, reach maximal values at 2 days, and remain above normal to some degree for at least 27 days. In addition, a small but significant increase in spectrin proteolysis is also observed in the hippocampus contralateral to the lesioned side in the first week postlesion. At 2 days postlesion the total spectrin immunoreactivity (native polypeptide plus breakdown products) increases by 40%, suggesting that denervation of the dentate gyrus produces not only an increased rate of spectrin degradation but also an increased rate of spectrin synthesis. These results indicate that calpain activation and spectrin degradation are early biochemical events following deafferentation and might well participate in the remodelling of postsynaptic structures. Finally, the magnitude of the observed effects as well as the stable nature of the breakdown products provide a sensitive assay for neuronal pathology.
authors.affiliation: Center for the Neurobiology of Learning and Memory, University of California, Irvine 92717.
authors.forename: P
authors.initials: P
authors.lastname: Seubert
authors.forename: G
authors.initials: G
authors.lastname: Ivy
authors.forename: J
authors.initials: J
authors.lastname: Larson
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: Shahi
authors.forename: M
authors.initials: M
authors.lastname: Baudry
authors.forename: G
authors.initials: G
authors.lastname: Lynch
mesh_terms: D000818:Animals; D002147:Calmodulin / Q000494:pharmacology; D002154:Calpain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007276:Injections, Intraventricular; D007976:Leupeptins / Q000494:pharmacology; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013049:Spectrin / Q000378:metabolism*
chemical_list: D002147:Calmodulin; D004791:Enzyme Inhibitors; D007976:Leupeptins; D013049:Spectrin; D002154:Calpain; C032854:leupeptin