The significance of complement activation in the pathogenesis of hypersensitivity pneumonitis: sequential changes of complement components and chemotactic activities in bronchoalveolar lavage fluids.
Hypersensitivity pneumonitis (HP) is believed to be induced by immunological mechanisms, the details of which remain to be clarified. While a role for cellular immunity is accepted in the pathogenesis of HP, several clinical observations also suggest a role for immune-complex-mediated lung injury. We have previously demonstrated the presence of chemotactic factors for polymorphonuclear cells (PMNs) in bronchoalveolar lavage (BAL) fluids of acutely ill patients with the summer type of HP found in Japan. The present study correlated chemotactic factors for PMNs with the level of C5a des Arg in BAL fluids obtained from patients with summer type HP. Furthermore, this study demonstrated that PMNs were increased in BAL fluids obtained after 2 days of avoidance of exposure to the presumptive causative agent. The percentage of PMNs in the BAL increased in proportion to the activity of the chemotactic factors. Finally, leukotriene B4 was not detected in concentrated BAL or supernatant fluids of cultured macrophages. These results suggest that complement activation in the respiratory tract may occur as the early event in the pathogenesis of HP.
Yoshizawa
Y
Y
Department of Internal Medicine, University of Tsukuba, Ibaraki, Japan.
Nomura
A
A
Ohdama
S
S
Tanaka
M
M
Morinari
H
H
Hasegawa
S
S
D000542:Alveolitis, Extrinsic Allergic / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology* / Q000473:pathology; D002630:Chemotactic Factors / Q000378:metabolism*; D003167:Complement Activation; D003176:Complement C3 / Q000031:analogs & derivatives / Q000032:analysis; D015926:Complement C3a / Q000031:analogs & derivatives*; D003182:Complement C5 / Q000031:analogs & derivatives / Q000032:analysis; D015937:Complement C5a, des-Arginine; D003165:Complement System Proteins / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8; D007975:Leukotriene B4 / Q000032:analysis; D009504:Neutrophils / Q000276:immunology; D011506:Proteins / Q000032:analysis
D002630:Chemotactic Factors; D003176:Complement C3; D003182:Complement C5; D015937:Complement C5a, des-Arginine; D016209:Interleukin-8; D011506:Proteins; C044541:complement C3a, des-Arg-(77)-; D007975:Leukotriene B4; D015926:Complement C3a; D003165:Complement System Proteins