Diuresis-dependent excretion of multiple forms of renal brush-border enzymes in urine.
The urinary brush-border enzymes alanine aminopeptidase (EC 3.4.11.2), alkaline phosphatase (EC 3.1.3.1) and gamma-glutamyltransferase (EC 2.3.2.2) and their activity pattern of soluble and particulate forms separated by ultracentrifugation were investigated over 8 h after a high intake of fluid (22 ml/kg body wt.). The activity pattern of the two multiple forms was markedly influenced by the urine flow rate. The time-related excretion of soluble forms of these three enzymes increased with the extent of urine output whereas the excretion of particulate form was independent on diuresis. Diuresis-dependent excretion of total activities of brush-border enzymes can be exclusively considered to be a consequence of changed excretion of soluble forms.
Jung
K
K
Schulze
G
G
D000469:Alkaline Phosphatase / Q000652:urine*; D000626:Aminopeptidases / Q000652:urine*; D018826:CD13 Antigens; D004231:Diuresis; D004326:Drinking; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D008871:Microvilli / Q000201:enzymology; D012995:Solubility; D005723:gamma-Glutamyltransferase / Q000652:urine*
D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D000626:Aminopeptidases; D018826:CD13 Antigens