title: Vanadium metabolism in wild type and respiratory-deficient strains of S. cerevisiae.
abstract: Vanadium metabolism was studied in a wild type and respiratory-deficient strain of S. cerevisiae. Inhibition of growth by vanadate [V(+5)], vanadate accumulation, and conversion of medium vanadate [V(+5)] to both cell-associated and medium vanadyl [V(+4)] and vanadate [V(+5)] were compared. The growth of both the parental and respiratory-deficient strains was inhibited by vanadate at concentrations greater than or equal to 1 mM. Both parental and respiratory-deficient strains accumulated vanadate and converted medium vanadate to cellular vanadyl as detected using electron spin resonance (ESR). The accumulation of cell-associated vanadyl was correlated with the loss of medium vanadate in both strains using a chemical assay. In contrast, the respiratory-deficient strain showed a greater amount of a cell-associated vanadate compound, as detected with vanadium-51 nuclear magnetic resonance (51V-NMR), than the wild type strain or a representative respiratory-competent vanadate-resistant mutant. These data imply that mitochondrial function may be directly involved in vanadium metabolism.
authors.affiliation: Department of Biochemistry, State University of New York, Buffalo 14214.
authors.forename: G R
authors.initials: GR
authors.lastname: Willsky
authors.forename: S F
authors.initials: SF
authors.lastname: Dosch
mesh_terms: D002455:Cell Division; D004578:Electron Spin Resonance Spectroscopy; D004734:Energy Metabolism; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D010085:Oxidative Phosphorylation; D011868:Radioisotopes; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014638:Vanadates / Q000378:metabolism; D014639:Vanadium / Q000378:metabolism*
chemical_list: D011868:Radioisotopes; D014639:Vanadium; D014638:Vanadates