title: Isolation of a bovine rotavirus with a "super-short" RNA electrophoretic pattern from a calf with diarrhea.
abstract: A rotavirus with a "super-short" RNA electropherotype was isolated from a calf with diarrhea and was designated VMRI strain. Segments 10 and 11 of this rotavirus migrated more slowly than did those of bovine rotavirus strains NCDV, B641, and B223. The electrophoretic pattern of the VMRI strain was similar to that reported for rotaviruses with super-short RNA electropherotypes from humans and rabbits. Northern (RNA) blot hybridization indicated that gene 11 of the VMRI strain was altered and migrated between gene segments 9 and 10. The subgroup of the VMRI strain was shown to be subgroup I. The VMRI strain of bovine rotavirus was neutralized by antisera containing polyclonal antibodies to rotavirus serotype 6 (bovine rotavirus serotype I) strains NCDV and B641 and by ascitic fluid containing monoclonal antibodies directed to VP7 of serotype 6 rotavirus. The VMRI strain was not neutralized by either polyclonal or monoclonal antibodies to strain B223 (bovine rotavirus serotype II). Collective data on the neutralization of the VMRI strain with monoclonal antibodies and polyclonal antibodies suggest that this virus is a member of the NCDV group (serotype 6) of rotaviruses (bovine rotavirus serotype I).
authors.affiliation: Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011.
authors.forename: P S
authors.initials: PS
authors.lastname: Paul
authors.forename: Y S
authors.initials: YS
authors.lastname: Lyoo
authors.forename: G N
authors.initials: GN
authors.lastname: Woode
authors.forename: S L
authors.initials: SL
authors.lastname: Zheng
authors.forename: H B
authors.initials: HB
authors.lastname: Greenberg
authors.forename: S
authors.initials: S
authors.lastname: Matsui
authors.forename: K J
authors.initials: KJ
authors.lastname: Schwartz
authors.forename: H T
authors.initials: HT
authors.lastname: Hill
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004586:Electrophoresis; D006168:Guinea Pigs; D009500:Neutralization Tests; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012703:Serotyping
chemical_list: D012367:RNA, Viral