title: [Cytomegalovirus (CMV): virologic protocols for transplantation].
abstract: After transplantation, the frequency of reactivation (or superinfection) by CMV is high. Less frequent, but more severe, is the primary form of the disease. The virological procedure to follow up the graft recipients includes: 1) The detection of the infectious virus and/or its antigens in urine, blood, bronchial secretions or brushing, bronchiolar and alveolar washings, ... The laboratory will perform a rapid culture for detection of early CMV antigen (24-72 h) associated with the more classical long-term culture, a reliable but slow method (2-3 weeks). 2) The detection of antibodies, primarily IgM, or even IgA. The usual ELISA for IgG and ELISA modified by addition of an immunocapture on a solid phase are the more reliable techniques. As a rule, the pattern IgM+/IgG- is common for the primary infection. In the case of reactivation (or superinfection), the pattern may be either IgM+/IgG+ or IgM-/IgG+. In the latter case, the increase of the IgG during the next weeks will bring evidence of a reactivation process.
authors.affiliation: Service de Virologie, Hôpital Nord, CHRU, Saint-Etienne.
authors.forename: O
authors.initials: O
authors.lastname: Gaudin
authors.forename: B
authors.initials: B
authors.lastname: Pozzetto
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D006801:Humans; D008828:Microbiological Techniques; D009894:Opportunistic Infections / Q000175:diagnosis; D012698:Serologic Tests; D013997:Time Factors; D014180:Transplantation
chemical_list: D000914:Antibodies, Viral
vernacular_title: Le cytomégalovirus (CMV): protocole virologique adapté à la transplantation.