Comparative histochemical investigation of the glutathione S-transferase placental form and gamma-glutamyltranspeptidase during N-nitrosobis(2-hydroxypropyl)amine-induced pancreatic carcinogenesis in hamsters.
Immunohistochemical staining using anti-rat glutathione S-transferase placental form (GST-P) rabbit antibody and enzyme histochemical staining for gamma-glutamyltranspeptidase (gamma-GT) were investigated in putative preneoplastic lesions and adenocarcinomas in the pancreas of Syrian golden hamsters treated with N-nitrosobis(2-hydroxypropyl)amine (BHP). Areas with ductular proliferation, ductal hyperplasia, and intraductal carcinoma were strongly positive for GST-P binding and negative for gamma-GT. Cystic adenoma, microcarcinoma, and carcinomas were constantly positively stained by GST-P and partially positive for gamma-GT. GST-P appears to be useful as a positive marker for putative preneoplastic lesions in pancreatic carcinogenesis. Since normal acinar cells are strongly positive for gamma-GT, the findings might suggest that acinar cells contribute to the development of cystic adenoma, microcarcinoma, and carcinomas.
Obara
T
T
Makino
T
T
Ura
H
H
Yokose
Y
Y
Kinugasa
T
T
Moore
M A
MA
Sato
K
K
Konishi
Y
Y
D000818:Animals; D006224:Cricetinae; D005982:Glutathione Transferase / Q000032:analysis* / Q000276:immunology; D006651:Histocytochemistry; D008297:Male; D008647:Mesocricetus; D009602:Nitrosamines; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D010920:Placenta / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D009602:Nitrosamines; C012457:diisopropanolnitrosamine; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase