Influence on cell-cell communication (dye-transfer) of the oncogenic beta-blocker DL-ZAMI 1305: possible relation to tumor promotion.
The effect of the oncogenic beta-blocker DL-1-(2-nitro-3-methyl-phenoxy)-3-tert-butyl-amino-propan-2-ol (DL-ZAMI 1305) on intercellular communication between cultured cells was studied. Intercellular communication of Chinese hamster V79 cells was measured by the dye-transfer method in which the spread of intracellularly microinjected fluorescent probe, Lucifer Yellow CH, through gap-junctions was used as an index of intercellular communication. When V79 cells are cultured with non-toxic doses (1-60 micrograms/ml) of DL-ZAMI 1305, a significant inhibition of dye-transfer is observed after 4 h. The inhibition is dose-related and greater than 90% inhibition is seen at the dose of 50 micrograms/ml. When DL-ZAMI 1305 is added at 0 and 24 h of experiment, its inhibitory effect is maintained for at least 48 h at high doses (50-60 micrograms/ml), whereas for lower doses of DL-ZAMI 1305, some recovery is seen after 24 h incubation. These results are suggestive of a possible tumor-promoting activity of DL-ZAMI 1305; in vivo studies on this carcinogen are in progress.
Mazzoleni
G
G
Ragnotti
G
G
Enomoto
T
T
Yamasaki
H
H
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002450:Cell Communication / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D007546:Isoquinolines; D011412:Propanolamines / Q000494:pharmacology*; D013997:Time Factors
D000319:Adrenergic beta-Antagonists; D002273:Carcinogens; D007546:Isoquinolines; D011412:Propanolamines; C028475:ZAMI 1305; C017475:lucifer yellow