Comparison of the effects of calcium alpha-glycerophosphate and Ca2+ on prostaglandin synthesis in rabbit kidney medulla slices.
The stimulatory effects of calcium alpha-glycerophosphate (CaG) and Ca2+ on the generation of medullary prostaglandin E2 have been compared. CaG (4mM) was significantly more effective than Ca2+ (4 mM) as a stimulus for prostaglandin E2 release. This effect of CaG was most pronounced in the presence of exogenous phospholipase A2 (0.1 unit/ml). These results suggest that CaG has a more powerful stimulatory effect on prostaglandin E2 formation than Ca2+ because of its high affinity for phospholipase A2.
Fujita
T
T
Kasatani
H
H
Suzuki
K
K
Fujimoto
Y
Y
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D015232:Dinoprostone; D005994:Glycerophosphates / Q000494:pharmacology*; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011458:Prostaglandins E / Q000096:biosynthesis*; D011817:Rabbits; D013997:Time Factors
D005994:Glycerophosphates; D011458:Prostaglandins E; C029620:alpha-glycerophosphoric acid; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone; D002118:Calcium