title: Insertion sequence IS2 in the cyanobacterium Chlorogloeopsis fritschii.
abstract: A cloned DNA fragment, previously demonstrated to encode ribulose bisphosphate carboxylase/oxygenase (RuBisCO) of Chlorogloeopsis fritschii strain CCAP1411/1b, is shown also to include the entire transposable element, IS2, normally a resident in the Escherichia coli genome. Southern-blot hybridisation experiments confirm the presence of IS2 in the C. fritschii genome. This finding adds a new and unrelated species to the known host range of this element and provides evidence of genetic transfer between the Gram-negative E. coli and cyanobacteria. This may also have significance in relation to the nucleotide sequence rearrangements known to occur adjacent to RuBisCO and nif genes in other nitrogen-fixing cyanobacteria.
authors.affiliation: Department of Biological Sciences, University of Dundee, U.K.
authors.forename: G C
authors.initials: GC
authors.lastname: Machray
authors.forename: D
authors.initials: D
authors.lastname: Vakeria
authors.forename: G A
authors.initials: GA
authors.lastname: Codd
authors.forename: W D
authors.initials: WD
authors.lastname: Stewart
mesh_terms: D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
chemical_list: D004251:DNA Transposable Elements; D004247:DNA