Target cells for the polychlorinated biphenyl metabolite 4,4'-bis(methylsulfonyl)-2,2',5,5'-tetrachlorobiphenyl in lung and kidney.
Light microscope autoradiography was used to determine the cellular localization of the polychlorinated biphenyl metabolite 4,4'-bis([14C] methylsulfonyl)-2,2',5-5'-tetrachlorobiphenyl ([3H]TCB) in the lung and kidney of mice and rats. Microautoradiograms prepared from thaw-mounted freeze sections showed that the radioactivity in the lung was localized in the bronchiolar lumen and epithelium. In methacrylate sections (74-82% of radioactivity extracted), a highly selective labeling was registered in the apical cytoplasm of the Clara cells. A pronounced labeling was present also in certain goblet-like cells containing periodic acid-Schiff-positive granules. Gel permeation chromatography and density gradient centrifugation showed that 80-95% of the radioactivity in lung lavage fluid was bound to a specific protein previously characterized in rat and mouse lung cytosol. The protein appeared to be enriched in the lavage fluid, as compared to lung cytosol. These data suggest that [3H]TCB binds to a protein residing in the Clara and goblet-like cells and that the labeled TCB-protein complex is subsequently secreted into the airway lumen. As shown by microautoradiography, the radioactivity in the kidney was confined to a restricted portion of the nephron, predominantly to the apical region of the proximal tubular cells.
Brandt
I
I
Lund
J
J
Bergman
A
A
Klasson-Wehler
E
E
Poellinger
L
L
Gustafsson
J A
JA
D000818:Animals; D001345:Autoradiography / Q000379:methods; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D005260:Female; D007668:Kidney / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D007507:Therapeutic Irrigation; D014018:Tissue Distribution
C044524:4,4'-bis(methylsulfonyl)-2,2',5,5'-tetrachlorobiphenyl; D011078:Polychlorinated Biphenyls