title: DNA conformational variations in the in vitro torsionally strained Ig kappa light chain gene localize on consensus sequences.
abstract: We have analyzed the localization and the dependence upon superhelical density of the DNA sites which modify their conformation under torsional strain in a mouse Ig L kappa gene. The conformational variations occur on DNA sites which have been defined as protein interaction sites and consensus sequence motifs: the 5'-upstream regulatory decanucleotides, the TATA sequence, the consensus heptanucleotides of the J recombinational sequences.
authors.affiliation: Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Venditti
authors.forename: M
authors.initials: M
authors.lastname: Caserta
authors.forename: E
authors.initials: E
authors.lastname: Di Mauro
authors.forename: G
authors.initials: G
authors.lastname: Camilloni
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D001483:Base Sequence; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004274:DNA, Recombinant / Q000378:metabolism; D004278:DNA, Superhelical / Q000187:drug effects / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009993:Osmium Tetroxide / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D001426:Bacterial Proteins; D004274:DNA, Recombinant; D004278:DNA, Superhelical; D007145:Immunoglobulin kappa-Chains; D004247:DNA; D004720:Endonucleases; C025580:endodeoxyribonuclease XBAI; C024404:BglII endonuclease; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D015719:Single-Strand Specific DNA and RNA Endonucleases; D009993:Osmium Tetroxide