[Identification of an atypical polyunsaturated fatty acid present in the liver of rats after the ingestion of heated linseed oil].
A C20:5 fatty acid with a trans delta 17 ethylenic bond was identified in liver lipids of rats fed with heated linseed oil. This component was isolated using a combination of high performance liquid chromatography on a reverse phase column and AgNO3 thin layer chromatography (TLC). This fatty acid was identified after hydrazine reduction, methoxy-bromomercuric adducts fractionation, AgNO3-TLC and ozonolysis in BF3-MeOH. It could be a metabolite of one trans isomeric linolenic acid formed during the heat treatment of linseed oil.
Piconneaux
A
A
Grandgirard
A
A
Sebedio
J L
JL
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004041:Dietary Fats / Q000008:administration & dosage* / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D006358:Hot Temperature / Q000009:adverse effects; D008042:Linolenic Acids / Q000378:metabolism; D008043:Linseed Oil / Q000008:administration & dosage* / Q000378:metabolism; D008099:Liver / Q000032:analysis*; D051381:Rats; D013237:Stereoisomerism; D017962:alpha-Linolenic Acid
D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D008042:Linolenic Acids; D017962:alpha-Linolenic Acid; D008043:Linseed Oil
Identification d'un acide gras polyinsaturé atypique présent dans le foie de rats ayant ingéré de l'huile de lin chauffée.