title: Disulfide-mediated interactions of the chlamydial major outer membrane protein: role in the differentiation of chlamydiae?
abstract: The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the replicative cell type, the reticulate body (RB). Treatment of EBs with dithiothreitol elicited a number of changes normally associated with differentiation to the RB. EBs in the presence of 10 mM dithiothreitol displayed enhanced rates of [14C]glutamate oxidation, reduced infectivity, and decreased osmotic stability, and their Machiavello staining properties changed to those characteristic of the RB. A true differentiation of EB to RB did not take place under these conditions, since EBs treated in this manner and examined by transmission electron microscopy did not demonstrate increased size or decreased electron density as do isolated RBs. Additional studies were initiated to identify the macromolecules involved in this process. With polyacrylamide gel electrophoresis and immunoblotting procedures with monoclonal and polyclonal monospecific antibodies, the chlamydial major outer membrane protein was found to be the predominant component that varied under reducing versus nonreducing conditions. Furthermore, the extent of disulfide-mediated cross-linking of the major outer membrane protein varied between the infective and replicative forms of the C. trachomatis LGV-434 life cycle. Implications of disulfide interactions in the life cycle of chlamydiae are discussed.
authors.forename: T
authors.initials: T
authors.lastname: Hackstadt
authors.forename: W J
authors.initials: WJ
authors.lastname: Todd
authors.forename: H D
authors.initials: HD
authors.lastname: Caldwell
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D002691:Chlamydophila psittaci / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D004220:Disulfides / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008854:Microscopy, Electron; D009995:Osmosis / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004220:Disulfides; D005971:Glutamates; D018698:Glutamic Acid; D004229:Dithiothreitol