Isolation of substances with vascular permeability-increasing activity from the skin of the plaice (Pleuronectes platessa L.).
A methanolic extract of plaice skin, from which lipids had been removed, was chromatographed on alumina, eluted with decreasing concentrations of ethanol. Only the 60% ethanol fraction exhibited smooth muscle activity, with bradykinin-like properties. The 20% ethanol fraction increased vascular permeability in rat skin, as measured by dye-leakage. This was not due to the degranulation of mast cells. Intradermal injection of either fraction into the plaice caused localized erythema.
Ashraf
A J
AJ
Smith
G M
GM
Fletcher
T C
TC
D000818:Animals; D001681:Biological Assay; D001794:Blood Pressure / Q000187:drug effects; D004487:Edema; D005260:Female; D005399:Fishes / Q000502:physiology*; D006168:Guinea Pigs; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010539:Permeability; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012879:Skin Physiological Phenomena; D014020:Tissue Extracts / Q000494:pharmacology*
D014020:Tissue Extracts