A possible mechanism whereby parathyroid hormone stimulates phospholipid synthesis in canine renal tubules.
Incubation of canine renal cortical tubules with parathyroid hormone and [3H]glycerol stimulated incorporation of tracer into phosphatidylinositol 4-phosphate and decreased phosphatidic acid labelling but did not affect phosphatidylinositol, phosphatidylcholine/phosphatidylserine, phosphatidylethanolamine/phosphatidylglycerol, 1,2-diacyl- or triacylglycerol labelling. The hormone did not change the activity of glycerol 3-phosphate acyltransferase or of phosphatidate phosphohydrolase measured in tubule homogenates. However, parathyroid hormone induced a rapid increase in phosphatidylinositol kinase activity associated with a decrease in Km of the enzyme for ATP in homogenized tubules. These findings suggest a possible mechanism whereby parathyroid hormone increases phospholipid synthesis in the renal cortex.
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Laboratorio de la Unidad Metabólica, Fundación Jiménez Díaz, Madrid, Spain.
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D019870:1-Phosphatidylinositol 4-Kinase; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004075:Diglycerides / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010711:Phosphatidate Phosphatase / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000096:biosynthesis / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000096:biosynthesis; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
C011439:1,2-diacylglycerol; D004075:Diglycerides; D010281:Parathyroid Hormone; D010712:Phosphatidic Acids; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010743:Phospholipids; D014280:Triglycerides; C037178:phosphatidylinositol 4-phosphate; D000255:Adenosine Triphosphate; D005992:Glycerol-3-Phosphate O-Acyltransferase; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase; D010711:Phosphatidate Phosphatase; D005990:Glycerol