title: Measurement of 3':5'cyclic AMP in biological samples using a specific monoclonal antibody.
abstract: A fast and sensitive radioimmunoassay for 3':5'cyclic AMP based on a monoclonal antibody has been worked out. Mice were immunized with protein-conjugated 2'-O-succinyl-3':5'-cyclic AMP. The monoclonal antibody detects 0.1 and 1 pmole cAMP with succinyl cAMP (125I)iodotyrosine methyl ester and (3H) cAMP, respectively, as tracers. It shows no cross-reactivity to other adenosine nucleotides up to the millimolar range; cGMP interferes only if present at a 500 fold excess. Plant and animal tissue samples as well as adenylate cyclase activity were analysed directly or after appropriate purification in case of interfering substances. Cyclic AMP levels measured in various tissues by the antibody binding assay correspond to those obtained by HPLC determination using fluorescent etheno-cAMP.
authors.affiliation: Institut für Biochemie, Universität Köln, FRG.
authors.forename: C
authors.initials: C
authors.lastname: Colling
authors.forename: R
authors.initials: R
authors.lastname: Gilles
authors.forename: M
authors.initials: M
authors.lastname: Cramer
authors.forename: N
authors.initials: N
authors.lastname: Nass
authors.forename: R
authors.initials: R
authors.lastname: Moka
authors.forename: L
authors.initials: L
authors.lastname: Jaenicke
mesh_terms: D000262:Adenylyl Cyclases / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D000242:Cyclic AMP / Q000032:analysis*; D008099:Liver / Q000032:analysis; D051379:Mice; D010944:Plants / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D000242:Cyclic AMP; D000262:Adenylyl Cyclases