title: Determination of delta 9-tetrahydrocannabinol in plasma using solid-phase extraction and high-performance liquid chromatography with electrochemical detection.
abstract: A method for the determination of nanogram amounts of delta 9-tetrahydrocannabinol (THC) in plasma and serum is described. THC was quantitatively isolated by solid-phase extraction after addition of an aqueous solution of urea and methanol to the sample. The extracts were analysed by high-performance liquid chromatography with electrochemical detection in the oxidizing mode. The detection limit of THC is ca. 100 pg for a signal-to-noise ratio of 3. With this method, levels of 2 ng/ml of THC in plasma can be measured.
authors.affiliation: Forensic Science Laboratory, Rijswijk, The Netherlands.
authors.forename: P G
authors.initials: PG
authors.lastname: Zweipfenning
authors.forename: J A
authors.initials: JA
authors.lastname: Lisman
authors.forename: A Y
authors.initials: AY
authors.lastname: van Haren
authors.forename: G R
authors.initials: GR
authors.lastname: Dijkstra
authors.forename: J J
authors.initials: JJ
authors.lastname: Holthuis
mesh_terms: D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000097:blood*; D004563:Electrochemistry; D006801:Humans
chemical_list: D013759:Dronabinol