Biosynthesis of various types of collagen by human hepatoma cells in vitro.
A cloned human hepatoma cell line (HH2-1) produced and formed collagen fibers in vitro. The relative rate of collagen synthesis by the cells was increased with an enhancement of the cell density. An analysis of the components of the collagen using sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the cells synthesized interstitial collagen, types I and III, and other collagenous proteins. Thus, human hepatoma cells may play an important role in the formation of stromal collagen in the tumor.
Uchino
R
R
Nohara
T
T
Okamoto
E
E
Fukumoto
M
M
Midorikawa
O
O
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D002999:Clone Cells; D003094:Collagen / Q000096:biosynthesis*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology
D003094:Collagen