The effects of Clostridium difficile crude toxins and purified toxin A on stripped rabbit ileal mucosa in Ussing chambers.
Clostridium difficile crude toxins and purified toxin A had similar effects on stripped rabbit ileal mucosa in Ussing chambers. Both toxin preparations caused secretion of sodium and chloride ions by increasing serosa to mucosa (s----m) fluxes. Transmural potential difference and resistance decreased after toxin treatment. Onset of changes in electrical measurements and ion fluxes coincided with onset of histological changes. The response to theophylline was greatly reduced in toxin-treated tissue compared with control tissue.
Mitchell
T J
TJ
Ketley
J M
JM
Burdon
D W
DW
Candy
D C
DC
Stephen
J
J
D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000633:toxicity*; D002712:Chlorides / Q000378:metabolism; D003013:Clostridium / Q000472:pathogenicity*; D004768:Enterotoxins / Q000633:toxicity*; D007082:Ileum / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects*; D008297:Male; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002712:Chlorides; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile; D012964:Sodium; D013806:Theophylline