title: Competitive binding of dynorphin-(1-13) and beta-endorphin to cerebroside sulfate in solution.
abstract: Circular dichroism was used as a probe for competitive binding of two opioid peptides, dynorphin-(1-13) and beta-endorphin, with cerebroside sulfate, a membrane lipid thought to be part of the morphine receptor complex. The rationale was that bound beta-endorphin is partially helical but bound dynorphin-(1-13) remains unordered, thus making it possible to detect the degree of binding of beta-endorphin. The addition of dynorphin-(1-13) to a cerebroside sulfate solution of beta-endorphin invariably displaced beta-endorphin from the peptide-lipid complex, but the addition of beta-endorphin had little effect on dynorphin-(1-13) bound to the lipid. Similar results were obtained for competitive binding of the two peptides with two other amphiphiles, sodium dodecyl and decyl sulfate. The maximum number of binding sites on dynorphin-(1-13) and beta-endorphin was between five and six, which coincides with the five positively charged side chains plus an alpha NH+3 group at the NH2 terminus on both peptide molecules. The results support our working hypothesis that dynorphin-(1-13) may displace beta-endorphin bound to the receptor, which in turn can account for the inhibition of beta-endorphin-induced analgesia by dynorphin-(1-13).
authors.forename: C S
authors.initials: CS
authors.lastname: Wu
authors.forename: N M
authors.initials: NM
authors.lastname: Lee
authors.forename: H H
authors.initials: HH
authors.lastname: Loh
authors.forename: J T
authors.initials: JT
authors.lastname: Yang
mesh_terms: D001667:Binding, Competitive; D002554:Cerebrosides; D002942:Circular Dichroism; D004399:Dynorphins; D004723:Endorphins; D010446:Peptide Fragments; D011199:Potentiometry; D011487:Protein Conformation; D011957:Receptors, Opioid; D013501:Surface-Active Agents; D001615:beta-Endorphin
chemical_list: D002554:Cerebrosides; D004723:Endorphins; D010446:Peptide Fragments; D011957:Receptors, Opioid; D013501:Surface-Active Agents; D001615:beta-Endorphin; C026843:dynorphin (1-13); D004399:Dynorphins