23Na nuclear magnetic resonance study of Na+-K+ pump inhibition by a fraction from uremic toxins.
An in vitro inhibitor of Na+/K+-transporting ATPase (EC 3.6.1.37) was isolated from uremic plasma and normal urine by liquid chromatography. A 23Na nuclear magnetic resonance study involving living erythrocytes showed that this inhibitor causes impairment of the Na+-K+ pump of intact erythrocytes. This finding may explain the high intra-erythrocytic sodium concentration in those uremic patients exhibiting a high concentration of this inhibitor. The presence of this same inhibitor in normal urine suggests that it may play a physiological role.
Gallice
P
P
Laboratoire de Biophysique, Faculté de Pharmacie, Marseille, France.
Monti
J P
JP
Baz
M
M
Murisasco
A
A
Crevat
A
A
D000818:Animals; D004285:Dogs; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D011506:Proteins / Q000302:isolation & purification*; D012975:Sodium Isotopes; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D014511:Uremia / Q000097:blood* / Q000652:urine; D000097795:ATPase Inhibitory Protein
D011506:Proteins; D012975:Sodium Isotopes; D000254:Sodium-Potassium-Exchanging ATPase; D000097795:ATPase Inhibitory Protein