title: Interactions of a nonneutralizing IgM antibody and complement in parainfluenza virus neutralization.
abstract: While many viruses activate the complement cascade directly, this is generally not a neutralizing event in the absence of antibody. We used a nonneutralizing IgM monoclonal antibody to parainfluenza virus type 3 (PIV3) hemagglutinin-neuraminidase (HN) to explore the role of antibody in complement-dependent neutralization of PIV3. Neither the antibody nor nonimmune guinea pig serum (GPS) neutralized PIV3 significantly, but a more than 100-fold reduction in titer was found when antibody and GPS were combined. Heat-inactivated GPS or GPS lacking either of two different complement proteins were all inactive with or without antibody. Specific repletion of the deficient sera with highly purified complement proteins restored neutralizing activity, indicating an absolute requirement for the classical pathway of complement activation and lytic terminal complement components, and viral lysis was confirmed by electron microscopy. The presence of antibody before complement activation was essential; later addition had no effect. Spontaneous complement activation by PIV3 occurred via the classical pathway in the absence of antibody. Addition of antibody did not alter the overall rate or extent of complement component C3 binding to PIV3 in these experiments. We conclude that certain nonneutralizing antibodies may support complement-dependent PIV3 neutralization by facilitating viral lysis. This process does not, however, involve enhanced activation through the C3 step. Lysis may require antibody-dependent localization of the membrane attack complex or reorganization of the viral envelope structures to facilitate attack complex insertion and lysis.
authors.affiliation: Laboratory of Clinical Investigation, NIAID, Bethesda, Maryland 20892.
authors.forename: S
authors.initials: S
authors.lastname: Vasantha
authors.forename: K L
authors.initials: KL
authors.lastname: Coelingh
authors.forename: B R
authors.initials: BR
authors.lastname: Murphy
authors.forename: R R
authors.initials: RR
authors.lastname: Dourmashkin
authors.forename: C H
authors.initials: CH
authors.lastname: Hammer
authors.forename: M M
authors.initials: MM
authors.lastname: Frank
authors.forename: L F
authors.initials: LF
authors.lastname: Fries
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity; D000937:Antigen-Antibody Reactions; D003171:Complement Pathway, Classical; D003165:Complement System Proteins / Q000276:immunology*; D015707:HN Protein; D007075:Immunoglobulin M / Q000276:immunology*; D009500:Neutralization Tests; D018108:Respirovirus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015707:HN Protein; D007075:Immunoglobulin M; D014759:Viral Envelope Proteins; D003165:Complement System Proteins