title: Autoradiographic localization of beta-adrenergic receptors in rat oviduct.
abstract: Catecholamines are known to have an inhibitory effect on oviductal smooth musculature by changing the adrenergic receptors activity. In order to further investigate the role of epinephrine and/or norepinephrine in oviduct, the distribution of beta-adrenergic receptors has been studied in the rat oviduct, using in vitro autoradiography and [125I]cyanopindolol (CYP), as radioligand. The specificity of the labelling and the characterization of receptor subtypes in different cell types was achieved by displacement of radioligand with increasing concentrations of zinterol, a beta-adrenergic agonist with preferential affinity for the beta 2-adrenoreceptor subtype and practolol, a beta-adrenergic antagonist that binds preferentially to beta 1-subtype. Quantitative estimation of ligand binding was achieved by densitometry. It was shown that the vast majority of beta-receptors were of the beta 2-subtype and were found in smooth muscle layers as well as in the epithelium. The latter localization suggests a role for epinephrine and/or norepinephrine on the oviductal epithelium.
authors.affiliation: MRC Group in Molecular Endocrinology, Laval University Medical Centre, Sainte-Foy, Que, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Tolszczuk
authors.forename: G
authors.initials: G
authors.lastname: Pelletier
mesh_terms: D000818:Animals; D001345:Autoradiography; D004848:Epithelium / Q000032:analysis / Q000648:ultrastructure; D005260:Female; D009132:Muscles / Q000032:analysis / Q000648:ultrastructure; D010057:Oviducts / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D011943:Receptors, Adrenergic, beta