Human papillomavirus type 52: a new virus associated with cervical neoplasia.
Analysis of biopsies of cervical intraepithelial neoplasia (CIN) revealed a high percentage of human papillomavirus (HPV) sequences that would hybridize to a mixture of HPV probes only under conditions of relaxed stringency. The DNA sequences of one of these viruses was molecularly cloned and shown to be a new HPV, type 52 (HPV-52). This virus is most closely related to HPV-33. Hybridization analysis with restriction fragments of HPV-52 showed collinearity with the HPV-33 genome. DNA sequencing revealed a high level of conservation between the two viruses within the L1 open reading frame but significant divergence in the non-coding region of the viral genomes. Prevalence studies indicated that HPV-52 sequences were present in three of 137 (2%) CIN and in one of 48 (2%) cervical squamous cell cancers studied in the U.S.A.
Shimoda
K
K
Department of Obstetrics and Gynecology, Vincent T. Lombardi Cancer Research Center, Georgetown University Medical Center, Washington, D.C. 20007.
Lorincz
A T
AT
Temple
G F
GF
Lancaster
W D
WD
D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D004279:DNA, Viral