title: Osteoblasts increase their rate of division and align in response to cyclic, mechanical tension in vitro.
abstract: Bone adapts to physical deformation in vivo, yet the mechanism of the adaptive process remains unknown. One reason for this perplexity has been the difficulty in examining the effects of a well-defined deformation regimen on individual bone cells. With the utilization of novel, flexible-bottomed cell culture plates, one can study the effects of cyclic strain on the morphologic and biochemical adaptations of individual osteoblasts in vitro. Avian, calvarial osteoblast-like cells, from passes 2-5, responded to cyclic strain, by increasing their rates of DNA synthesis and cell division during the first 72 h after initiation of a continuous deformation regimen comprised of 3 cycles per min of 0-24% elongation. In addition, within hours after initiation of the deformation regimen, cells oriented 90 degrees to the applied strain field at the periphery of the culture plate in the region of maximum strain and elongation.
authors.affiliation: Department of Oral Surgery, University of North Carolina, Chapel Hill 27599.
authors.forename: M J
authors.initials: MJ
authors.lastname: Buckley
authors.forename: A J
authors.initials: AJ
authors.lastname: Banes
authors.forename: L G
authors.initials: LG
authors.lastname: Levin
authors.forename: B E
authors.initials: BE
authors.lastname: Sumpio
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: R
authors.initials: R
authors.lastname: Jordan
authors.forename: J
authors.initials: J
authors.lastname: Gilbert
authors.forename: G W
authors.initials: GW
authors.lastname: Link
authors.forename: R
authors.initials: R
authors.lastname: Tran Son Tay
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000096:biosynthesis; D002455:Cell Division; D002645:Chickens; D000242:Cyclic AMP / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D007700:Kinetics; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015675:Osteocalcin; D010281:Parathyroid Hormone / Q000494:pharmacology; D051381:Rats; D013314:Stress, Mechanical; D014160:Transducers, Pressure; D014407:Tumor Cells, Cultured
chemical_list: D002135:Calcium-Binding Proteins; D010281:Parathyroid Hormone; D015675:Osteocalcin; D004247:DNA; D000242:Cyclic AMP; D002117:Calcitriol