Antigenic analysis of the Epstein-Barr virus major membrane antigen (gp350/220) expressed in yeast and mammalian cells: implications for the development of a subunit vaccine.
The Epstein-Barr virus (EBV) major surface membrane antigen, gp350/220, was expressed in recombinant yeast cells and in several recombinant mammalian cell lines. Each of the expressed proteins was analyzed for its ability to bind to a panel of anti-gp350/220 monoclonal antibodies and to a series of anti-EBV positive human sera. The antigens also were used as immunogens for the immunization of rabbits. Each expressed protein was found to be unique both in its pattern of reactivity to the various antibodies and in the spectrum of antibody induced following animal immunization. These results suggest that cell-specific post-translational modifications critically influence the antigenic presentation of the expressed proteins. Nonetheless, all of the mammalian cell-derived versions of the membrane antigen were found capable of inducing EBV-specific neutralizing antibodies.
Emini
E A
EA
Department of Virus and Cell Biology, Merck, Sharp, and Dohme Research Laboratories, West Point, Pennsylvania 19486.
Schleif
W A
WA
Armstrong
M E
ME
Silberklang
M
M
Schultz
L D
LD
Lehman
D
D
Maigetter
R Z
RZ
Qualtiere
L F
LF
Pearson
G R
GR
Ellis
R W
RW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004854:Herpesvirus 4, Human / Q000276:immunology*; D007739:L Cells; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D051381:Rats; D012441:Saccharomyces cerevisiae; D014612:Vaccines / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000235:genetics*; D014709:Vero Cells; D014763:Viral Matrix Proteins; D014765:Viral Vaccines / Q000235:genetics*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D008562:Membrane Glycoproteins; D014612:Vaccines; D014614:Vaccines, Synthetic; D014763:Viral Matrix Proteins; D014765:Viral Vaccines