Alterations in the synthesis of proteins in hepatocytes of rainbow trout fed cyclopropenoid fatty acids.
Hepatocytes from rainbow trout reared on a diet containing cyclopropenoid fatty acids were analyzed for alterations in protein composition and synthesis by double label experiments. Both cytosolic and microsomal hepatocyte fractions were investigated. In the cytosolic fraction, the synthesis of proteins in the range of 68,000 to 74,000 daltons were significantly decreased. The identity of these proteins remains uncertain. A pronounced depression in both the mass and apparent synthesis of a 200,000 to 240,000 dalton microsomal protein was also observed. Immunoblotting with antibodies raised against goose acetyl-CoA carboxylase and avidin-peroxidase staining suggest that this protein is acetyl-CoA carboxylase. Moreover, synthesis of this protein as well as mass of the protein in cyclopropenoid fatty acid-fed fish were less than 20% of that found in control fish.
Perdew
G H
GH
Schaup
H W
HW
Becker
M M
MM
Williams
J L
JL
Selivonchick
D P
DP
D000103:Acetyl-CoA Carboxylase / Q000276:immunology; D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D005227:Fatty Acids / Q000008:administration & dosage* / Q000378:metabolism; D006651:Histocytochemistry; D007163:Immunosorbent Techniques; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
D004041:Dietary Fats; D005227:Fatty Acids; D000103:Acetyl-CoA Carboxylase; D007930:Leucine