title: Studies on nuclear acid deoxyribonucleases and proofreading exonuclease isolated from rat liver nuclei.
abstract: Three kinds of deoxyribonucleases (peaks A, B, and C) were separated from purified rat liver nuclei on DEAE-cellulose column chromatography, and their characteristics were partially studied. Peaks A and B had endonuclease activities under acidic conditions with low substrate specificity and did not require divalent metal ions. Peak C had an exonuclease activity under alkaline conditions with substrate specificity for denatured DNA or single stranded homopolymer and required divalent cations. Peak C degraded 3'-terminally mismatched substrate much faster than 3'-terminally matched substrate.
authors.affiliation: Department of Health Sciences, Faculty of Integrated Arts and Sciences, University of Tokushima.
authors.forename: H
authors.initials: H
authors.lastname: Akiyoshi
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D003851:Deoxyribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D004720:Endonucleases; D005092:Exonucleases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D009691:Nucleic Acid Denaturation; D051381:Rats; D013379:Substrate Specificity
chemical_list: D003851:Deoxyribonucleases; D004720:Endonucleases; D005092:Exonucleases; D008274:Magnesium