title: Quantity of dengue virus required to infect rhesus monkeys.
abstract: As part of a dengue vaccine study, it was necessary to determine how much virus was required to infect rhesus monkeys. Serial dilutions of dengue 2 and 4 viruses were inoculated subcutaneously into groups of five monkeys and seroconversions determined on days 30 and 60 post-inoculation. The viruses were also titrated simultaneously in LLC-MK2 cells and mosquitoes. It was calculated that 9.5 mosquito infectious doses 50 (MID50) of dengue 2 virus and 22 MID50 of dengue 4 virus were required to infect 50% of the monkeys. The data suggest that 100 MID50 of dengue virus should be used as a challenge dose for monkeys previously immunized with dengue vaccine.
authors.forename: E
authors.initials: E
authors.lastname: Kraiselburd
authors.forename: D J
authors.initials: DJ
authors.lastname: Gubler
authors.forename: M J
authors.initials: MJ
authors.lastname: Kessler
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D009033:Culicidae / Q000382:microbiology; D003716:Dengue Virus / Q000276:immunology / Q000472:pathogenicity*; D008253:Macaca mulatta; D010948:Viral Plaque Assay
chemical_list: D000914:Antibodies, Viral