Cytotoxicity of 2-chlorodeoxyadenosine in a human tumor colony-forming assay.
We have utilized a human tumor colony forming assay to test the antitumor activity of 2-chlorodeoxyadenosine and to compare its activity with that of 9-beta-D-arabinofuranosyl-2-fluoroadenine, a related analog now in phase I/II clinical trials. The overall in vitro response rate (defined as less than 50% survival of tumor colony forming units) for 2-chlorodeoxyadenosine was: 8% and 23% at 1.0 and 10.0 micrograms/ml as a 1 hour pulse exposure, respectively; 11% and 31% at 1.0 and 10.0 micrograms/ml, as a continuous exposure, respectively. 2-Chlorodeoxyadenosine and 9-beta-D-arabinofuranosyl-2-fluoroadenine did not have identical spectra of antitumor activities in vitro, suggesting that both may be worthy of further clinical trial.
Hutton
J J
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Von Hoff
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D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017338:Cladribine; D003114:Colony-Forming Units Assay; D003839:Deoxyadenosines / Q000031:analogs & derivatives* / Q000633:toxicity; D004353:Drug Evaluation, Preclinical; D005260:Female; D006801:Humans; D007700:Kinetics; D009369:Neoplasms / Q000473:pathology*; D014740:Vidarabine / Q000031:analogs & derivatives / Q000633:toxicity
D003839:Deoxyadenosines; D017338:Cladribine; D014740:Vidarabine; C024352:fludarabine