The platelet insulin receptor: detection, partial characterization, and search for a function.
A direct demonstration and partial characterization of the insulin receptor on human platelets was obtained by immunoprecipitation with a monoclonal antibody against the human insulin receptor. Two subunits were detected, one with a MW of 95 KD, which dose-dependently was phosphorylated upon challenge of the platelets with insulin and another with a MW of 69 KD that does not become phosphorylated. Retention on wheat germ agglutinin indicates that at least part of the receptor protein is glycosylated. In a search for cellular effects provoked by insulin on platelets, no changes could be detected in cAMP formation or degradation, inositol phosphate formation or phosphorylation of proteins other than the receptor itself.
Falcon
C
C
Center for Thrombosis and Vascular Research, University of Leuven, Belgium.
Pfliegler
G
G
Deckmyn
H
H
Vermylen
J
J
D000262:Adenylyl Cyclases / Q000378:metabolism; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis; D011464:Epoprostenol / Q000494:pharmacology; D006031:Glycosylation; D006801:Humans; D007163:Immunosorbent Techniques; D007295:Inositol Phosphates / Q000096:biosynthesis; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology; D008970:Molecular Weight; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011972:Receptor, Insulin / Q000032:analysis* / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007295:Inositol Phosphates; D007328:Insulin; D010750:Phosphoproteins; D011464:Epoprostenol; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin; D010738:Type C Phospholipases; D000262:Adenylyl Cyclases