Coated vesicles purified from chick tendon fibroblasts contain newly synthesized type I procollagen.
Coated vesicles were purified from embryonic chick tendon fibroblasts pulsed with [3H]proline. They were morphologically and biochemically similar to coated vesicles purified from other sources. Furthermore, they contained newly synthesized Type I procollagen which was protected from bacterial collagenase digestion unless detergent was present. The procollagen remained associated with coated vesicles during immune precipitation and agarose gel electrophoresis. Data from pulse-chase experiments demonstrated that the specific activity of the coated vesicle preparations was approx. 5-fold higher at the 10 min chase point than at either the 0 or 40 min chase points. These data are consistent with the hypothesis that coated vesicles are intermediates in the intracellular transport of newly synthesized Type I procollagen in chick tendon fibroblasts.
Goldenberg
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Fine
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D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods; D011232:Chemical Precipitation; D002642:Chick Embryo; D002966:Clathrin / Q000032:analysis; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D011992:Endosomes / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D007120:Immunochemistry; D003012:Microbial Collagenase; D010455:Peptides / Q000032:analysis; D010779:Photofluorography; D011347:Procollagen / Q000096:biosynthesis / Q000378:metabolism*; D013710:Tendons
D002966:Clathrin; D010455:Peptides; D011347:Procollagen; D003012:Microbial Collagenase