Protein kinase C activities are increased in rat thyroid epithelial cells expressing v-ras genes.
Both cytoplasmic and membrane-bound protein kinase C activities are increased in: Harvey-Sarcoma Virus, infected thyroid epithelial cells. The cytoplasmic kinase C increase is found in the chromatographic fraction eluted at lower salt concentration (100 mM NaCl-S100), while the more acidic protein fraction eluted at higher salt concentration (35 mM NaCl-S350) is virtually absent. Although the cytoplasmic S100 fraction from the control and ras-virus infected cells display a comparable PBt2 binding activity, they are different in the Ca+2-dependence and the TPA down regulation. In addition, the membranes from the control and ras-virus infected cells are different phosphate acceptors in place of the H1 histones.
Spina
A
A
Cattedra di Chimica Biologica, Dipartimento di Biochimica e Biofisica, Università di Napoli, Italy.
Chiosi
E
E
Illiano
G
G
Berlingieri
M T
MT
Fusco
A
A
Grieco
M
M
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D002472:Cell Transformation, Viral; D002845:Chromatography; D003593:Cytoplasm / Q000201:enzymology; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004848:Epithelium / Q000201:enzymology; D011905:Genes, ras; D006251:Harvey murine sarcoma virus / Q000235:genetics; D009154:Mutation; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism; D010718:Phosphatidylserines / Q000494:pharmacology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D009053:Sarcoma Viruses, Murine / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013961:Thyroid Gland / Q000201:enzymology*
D004075:Diglycerides; D010718:Phosphatidylserines; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium; C013965:diolein