Isolation and characterization of monoclonal antibodies against an attenuated vaccine strain of equine herpesvirus type 1 (EHV-1).
The production and differentiation of monoclonal antibodies (mabs) against the Rac-H strain of EHV-1 used as an attenuated live vaccine to prevent rhinopneumonitis and abortion is described. Seven different antigenic sites were detected by the 15 mabs produced. EHV-1 specific mabs as well as EHV-1 and -4 common mabs could be established, allowing easy typing of EHV isolates. One mab recognized the vaccine strain only. This reaction was used to investigate a possible involvement of the vaccine strain in cases of abortion. Common antigenic determinants with EHV-1,-3,-4 and BHV-1 could also be detected, indicating the presence of highly-conserved epitopes of alpha-herpesviruses.
Meyer
H
H
Institute of Medical Microbiology, Veterinary Faculty, Ludwig-Maximilians-Universität, München, F.R.G.
Hübert
P H
PH
D000034:Abortion, Veterinary / Q000209:etiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006564:Herpesviridae / Q000276:immunology*; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D006734:Horse Diseases / Q000209:etiology; D006736:Horses; D006825:Hybridomas; D011247:Pregnancy; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines