Construction of a human cytochrome c gene and its functional expression in Saccharomyces cerevisiae.
The nucleotide sequences of a partial cDNA and three pseudogenes of human cytochrome c were determined. The complete nucleotide sequences which encode human cytochrome c were constructed on the basis of one of the pseudogenes by in vitro mutagenesis. The constructed human cytochrome c was functionally expressed in Saccharomyces cerevisiae. The recombinant human cytochrome c was purified and characterized.
Tanaka
Y
Y
Institute for Fundamental Research, Suntory, Ltd., Osaka.
Ashikari
T
T
Shibano
Y
Y
Amachi
T
T
Yoshizumi
H
H
Matsubara
H
H
D001483:Base Sequence; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000235:genetics*; D004247:DNA / Q000032:analysis; D005815:Genetic Code; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011544:Pseudogenes; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D003574:Cytochrome c Group; D004247:DNA