Immunohistochemical study of carcinoembryonic antigen (CEA) in lung carcinoma by five monoclonal antibodies.
The expression of carcinoembryonic antigen (CEA) and related antigens in 40 formalin-fixed paraffin embedded tissues of 4 histological types of lung carcinomas was examined immunohistochemically using five monoclonal antibodies (MoABs) which distinguished different epitopes on CEA, and the correlation with serum-CEA (s-CEA) level and tissue-CEA (t-CEA) was also investigated. Four MoABs detecting different peptide epitopes of CEA and another MoAB recognizing the carbohydrate part of CEA were used. Although each MoAB showed different frequencies of immunohistochemical expression according to the histological types, a similar tendency was generally suggested in four MoABs that recognized peptide epitopes of CEA except for one MoAB that recognized a carbohydrate epitopes of CEA. There was no difference among the five MoABs in regard to the staining localization exhibited by MoABs, but there was a difference among the four histological types. Elevation of s-CEA did not always occur in patients with lung cancers showing positive reaction to t-CEA, especially in adenocarcinoma and squamous cell carcinoma, but all patients with lung cancers showing intense positive reaction to t-CEA showed high degrees of elevation of s-CEA.
Hiraoka
K
K
Department of Pathology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Horie
A
A
D000230:Adenocarcinoma / Q000276:immunology; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D018288:Carcinoma, Small Cell / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008175:Lung Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen