Structure of a gap junction gene: rat connexin-32.
A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5'-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3'-end of the intron.
Miller
T
T
Department of Biochemistry, University of Miami, FL 33101.
Dahl
G
G
Werner
R
R
D000818:Animals; D001483:Base Sequence; D002999:Clone Cells; D017630:Connexins; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D051381:Rats; D015183:Restriction Mapping
D017630:Connexins; D015342:DNA Probes; D008565:Membrane Proteins; D015345:Oligonucleotide Probes; D004247:DNA