The action of LPS porins and peptidoglycan fragments on human spermatozoa.
This study examines the action of the cell wall components of enterobacteria on the vitality of human spermatozoa. Lipopolysaccharides extracted from Escherichia coli K12 killed about 80% of the spermatozoa at a concentration of 50 micrograms/ml. Porins extracted from E. coli, Proteus mirabilis and Salmonella typhimurium killed between 80% and 100% of the spermatozoa at a concentration of 50 micrograms/ml. Muramic acid and N-acetylmuramic acid caused about 60% mortality at a concentration of 50 micrograms/ml. The possibility that the products of cellular lysis in the course of gram-negative infections cause temporary sterility is discussed.
Galdiero
F
F
Istituto di Microbiologia, I Facoltà di Medicina e Chirurgia Università di Napoli.
Gorga
F
F
Bentivoglio
C
C
Mancuso
R
R
Galdiero
E
E
Tufano
M A
MA
D001425:Bacterial Outer Membrane Proteins / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D010457:Peptidoglycan / Q000494:pharmacology*; D018272:Porins; D011513:Proteus mirabilis; D012486:Salmonella typhimurium; D013094:Spermatozoa / Q000187:drug effects*
D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D018272:Porins