title: High molecular weight microtubule-associated proteins: purification by electro-elution and amino acid compositions.
abstract: The high molecular weight microtubule-associated proteins (MAPs) consist of MAPs 1A, 1B, 1C, 2A, and 2B. The proteins have been prepared from rat brain by using taxol and resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The individual proteins were purified by electro-elution from stained fixed gels and shown to migrate at their original mobility without proteolysis. Amino acid compositions of purified MAP 1 and 2 component proteins are reported and compared.
authors.forename: K S
authors.initials: KS
authors.lastname: Kosik
authors.forename: S F
authors.initials: SF
authors.lastname: Bakalis
authors.forename: D J
authors.initials: DJ
authors.lastname: Selkoe
authors.forename: M W
authors.initials: MW
authors.lastname: Pierce
authors.forename: L K
authors.initials: LK
authors.lastname: Duffy
mesh_terms: D000470:Alkaloids; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000302:isolation & purification*; D008970:Molecular Weight; D017239:Paclitaxel; D051381:Rats
chemical_list: D000470:Alkaloids; D000596:Amino Acids; D008869:Microtubule-Associated Proteins; D017239:Paclitaxel