title: Escherichia coli serotype K44: an acidic capsular polysaccharide containing two 2-acetamido-2-deoxyhexoses.
abstract: The structure of the capsular polysaccharide from Escherichia coli O8:K44 (A):H- (K44 antigen) has been established using the techniques of methylation, beta-elimination, deamination, and Smith degradation. N.m.r. spectroscopy (13C and 1H) was used extensively to establish the nature of the anomeric linkages of the polysaccharide and of oligosaccharides derived through degradative procedures. The K antigen is comprised of repeating units of the linear tetrasaccharide shown. This acidic polysaccharide represents the first instance of an E. coli K antigen in this series (group A) that has been found to contain two different 2-acetamido-2-deoxyhexoses.
authors.affiliation: Department of Chemistry, University of British Columbia, Vancouver, Canada.
authors.forename: G G
authors.initials: GG
authors.lastname: Dutton
authors.forename: D N
authors.initials: DN
authors.lastname: Karunaratne
authors.forename: A V
authors.initials: AV
authors.lastname: Lim
mesh_terms: D002234:Carbodiimides; D002840:Chromates; D003641:Deamination; D004926:Escherichia coli / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012703:Serotyping
chemical_list: D002234:Carbodiimides; D002840:Chromates; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; C009288:metaperiodate; C034944:chromic acid