Induction of simian virus 40 transplantation immunity in hamsters by gel-purified SV40 large T antigen.
Simian virus 40 (SV40) large T antigen and p53 cellular protein were isolated from an SV40-transformed hamster cell line by immunoprecipitation with anti-T sera and purified by sodium dodecyl sulfate-gel electrophoresis. These two protein were tested in hamsters for the presence of SV40 transplantation rejection antigenic sites by in vivo transplantation rejection assay. The large T antigen immunized the hamsters against a challenge of SV40 tumor cells and the protected animals generated cytotoxic spleen cells. Hamsters immunized with the p53 cellular protein were not protected against SV40-induced tumor but there was some delay in the appearance of tumor.
Haddada
H
H
Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Le Goffic
N
N
Chandrasekaran
K
K
de Vaux Saint Cyr
C
C
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology* / Q000302:isolation & purification; D002461:Cell Line, Transformed; D006224:Cricetinae; D006084:Graft Rejection; D007114:Immunization; D008647:Mesocricetus; D009363:Neoplasm Proteins / Q000276:immunology; D009368:Neoplasm Transplantation; D010750:Phosphoproteins / Q000276:immunology; D013539:Simian virus 40 / Q000276:immunology*; D016159:Tumor Suppressor Protein p53
D000952:Antigens, Polyomavirus Transforming; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53