[Uncoordinated expression of B2+mRNAx and other types of B2-containing poly(A)+RNA in various mouse tissues].
Northern blot hybridization of nuclear, cytoplasmic and polysomal poly(A)+RNAs from different normal and tumor mouse cells was carried out with labelled separated DNA strands of the cloned cDNA fragment containing repetitive B2-element. Several important results were obtained in these experiments: (i) while nuclear RNAs contain copies from both strands of B2 element, only one strand ("canonical" orientation) of B2 can be detected in the mature cytoplasmic poly(A)+RNAs of all mouse tissues studied, (ii) the abundant 2 kb B2+mRNAx is tissue-specific for mouse liver and kidney cells, its transcription is greatly increased in regenerating liver and completely abolished in hepatoma, (iii) an elevated level of small B2+RNAs and 1.6 kb B2+RNA in some tumor cells is observed, (iv) differential transcription of the major classes of B2+RNAs in different mouse tissues suggests the non-coordinated regulation of their expression.
Ivanov
P L
PL
Tokarskaia
O N
ON
Kramerov
D A
DA
Grigorian
M S
MS
Ryskov
A P
AP
D000818:Animals; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D008114:Liver Neoplasms, Experimental / Q000032:analysis / Q000235:genetics; D051379:Mice; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D012335:RNA, Ribosomal / Q000032:analysis / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014018:Tissue Distribution; D014158:Transcription, Genetic
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D011061:Poly A
Nekoordinirovannaia ékspressiia B2+mRNKx i drugikh tipov B2-soderzhashchikh poli(A)+RNK v razlichnykh tkaniakh myshi.