Cyclic nucleotides fail to affect mucus glycoconjugate secretion from canine tracheal explants.
Canine tracheal explants, incubated overnight with [3H]glucosamine, elicited an enhanced secretion of ethanol-precipitated [3H]labelled glycoconjugate when challenged with methacholine, 10 microM. Neither the beta-adrenoceptor agonist isoprenaline, 10 microM, nor the phosphodiesterase inhibitor theophylline, 10 mM, had any significant effect on glycoconjugate secretion. Dibutyryl cyclic AMP, 1 mM, and dibutyryl cyclic GMP, 1 mM, alone or in combination with theophylline, 10 mM, were devoid of activity on unstimulated or methacholine-stimulated tracheal explants. The calcium ionophore A23187, 10 microM, stimulated [3H]glycoconjugate secretion from each of the tissues tested; however, the cyclic nucleotides failed to modify this response. These data indicate that the cyclic nucleotides play little, if any, role in mucus glycoconjugate secretion by the canine trachea.
Barbieri
E J
EJ
Barsigian
C
C
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D006152:Cyclic GMP / Q000031:analogs & derivatives*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology*; D004285:Dogs; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008297:Male; D008688:Methacholine Compounds / Q000494:pharmacology; D009093:Mucus / Q000378:metabolism*; D009924:Organ Culture Techniques; D014132:Trachea / Q000378:metabolism*
D008688:Methacholine Compounds; D003995:Dibutyryl Cyclic GMP; D000001:Calcimycin; D003994:Bucladesine; D006152:Cyclic GMP; D005947:Glucose; D005944:Glucosamine