NMR evidence for an early framework intermediate on the folding pathway of ribonuclease A.
The presence of an early intermediate on the folding pathway of ribonuclease A has been demonstrated by a study of the exchange reaction between the backbone amide protons in the folding protein and solvent protons using rapid mixing techniques. A structural analysis of the intermediate by two-dimensional 1H-NMR is consistent with the framework model of protein folding in which stable secondary structure first forms the framework necessary for the subsequent formation of the complete tertiary structure.
Udgaonkar
J B
JB
Department of Biochemistry, Stanford University School of Medicine, California 94305.
Baldwin
R L
RL
D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011487:Protein Conformation; D011522:Protons; D012259:Ribonuclease, Pancreatic
D011522:Protons; D012259:Ribonuclease, Pancreatic