Induction of anchorage-independent growth of human embryonic fibroblasts with a deletion in the short arm of chromosome 11 by human papillomavirus type 16 DNA.
Human embryonic fibroblasts with a large deletion (11p11.11p15.1) in the short arm of one chromosome 11 (del-11 cells) appeared to be susceptible to transformation by early human papillomavirus type 16 (HPV-16) DNA, whereas diploid human embryonic fibroblasts were not. This difference in susceptibility might be explained by the absence of a tumor suppressor gene located within the deleted part on the short arm of chromosome 11. The presence of abundant viral early-gene transcripts in transformed cells suggests that transformation was induced by an elevated level of an HPV-16 early-gene product(s). The low transcriptional activity of HPV-16 in diploid cells may indicate that cellular genes affect viral transcription. Interruption of the HPV-16 E2 early open reading frame is probably required for high-level HPV-16 early-gene expression driven from the homologous enhancer-promoter region.
Smits
H L
HL
Department of Medical Microbiology, Academic Medical Centre, Amsterdam, The Netherlands.
Raadsheer
E
E
Rood
I
I
Mehendale
S
S
Slater
R M
RM
van der Noordaa
J
J
ter Schegget
J
J
D015139:Blotting, Southern; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002872:Chromosome Deletion; D002880:Chromosomes, Human, Pair 11; D004279:DNA, Viral / Q000235:genetics*; D004171:Diploidy; D005347:Fibroblasts; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis; D014158:Transcription, Genetic; D014162:Transfection
D004279:DNA, Viral; D012367:RNA, Viral