Beta-adrenergic regulation of c-fos gene expression in an epithelial cell line.
Stimulation of beta-adrenoreceptors in the RSMT-A5 epithelial cell line is accompanied by an early and transient increase in the expression of the proto-oncogene c-fos. Maximal induction was at 30 min, returning to basal levels after 2 h. Similar results were obtained when cells were incubated with 8-bromo-cAMP. The induction of c-fos is specific since the expression of p53, a transformation-related gene, is not modulated by isoproterenol or 8-bromo-cAMP. The increase in c-fos gene expression is not associated with proliferative activity in these epithelial cells.
Yeh
C K
CK
Clinical Investigations and Patient Care Branch, National Institute of Dental Research, Bethesda, MD 20892.
Louis
J M
JM
Kousvelari
E E
EE
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004848:Epithelium / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D016159:Tumor Suppressor Protein p53
D009363:Neoplasm Proteins; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D016159:Tumor Suppressor Protein p53; D015124:8-Bromo Cyclic Adenosine Monophosphate; D004247:DNA; D007545:Isoproterenol