title: Diagnosis of giardiasis by specific IgM antibody enzyme-linked immunosorbent assay.
abstract: An enzyme-linked immunosorbent assay (ELISA) was used to detect serum IgM and IgG antibodies against Giardia in fifty-two patients with diarrhoea from the United Kingdom and south India. Serum anti-Giardia IgM responses occurred in patients with giardiasis; in two of three patients studied longitudinally IgM levels had fallen to normal 2-3 weeks after treatment. At serum dilutions of 1/200 to 1/400 there was almost complete separation of ELISA readings between patients with Giardia and Giardia-free controls; the sensitivity and specificity of the ELISA were both 96%. Serum anti-Giardia IgG was detected in patients and controls but was unable to distinguish current from previous infection.
authors.forename: A K
authors.initials: AK
authors.lastname: Goka
authors.forename: D D
authors.initials: DD
authors.lastname: Rolston
authors.forename: V I
authors.initials: VI
authors.lastname: Mathan
authors.forename: M J
authors.initials: MJ
authors.lastname: Farthing
mesh_terms: D000208:Acute Disease; D000906:Antibodies / Q000032:analysis*; D003967:Diarrhea / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005873:Giardiasis / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D007194:India; D008137:Longitudinal Studies; D006113:United Kingdom
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M