Molecular dynamics in protein-single stranded DNA complexes. Two distinct nucleoside mobilities, in poly(deoxythymidylic acid)-poly-L-lysine complexes.
Stoichiometric amounts of poly-L-lysine were added to site-specifically spin labeled single stranded nucleic acids and the resulting complexes analyzed by electron spin resonance spectroscopy (ESR). The nucleic acids were spin labeled to different extents and with labels of varying tether length. The ESR data are used to determine nucleoside dynamics and some structural features in these complexes. It is concluded that two distinct base mobilities exist in the complexes; one set is characterized by a mean correlation time tau -R = 2 ns, and the other one by a tau -R greater than or equal to 50 ns. A model is proposed which suggests that a poly-L-lys single stranded nucleic acid complex consists of low mobility segments flanked by more mobile bases. An interesting feature of the proposed model is its applicability to explain ESR data of single strand binding protein-spin labeled nucleic acid complexes, which can also be interpreted in terms of two distinct nucleoside mobility states. It is hypothesized that this phenomenon could be of biological significance for the release of protein ligands from a protein-nucleic acid complex.
Bobst
A M
AM
Department of Chemistry, University of Cincinnati, Ohio 45221.
Kao
S C
SC
Bobst
E V
EV
Pauly
G T
GT
D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy; D008958:Models, Molecular; D011071:Poly T; D011089:Polydeoxyribonucleotides; D011107:Polylysine; D011485:Protein Binding
D011089:Polydeoxyribonucleotides; D011071:Poly T; D011107:Polylysine