Effect of haemorrhagic shock and duodenal instillation of blood on the plasma profiles of somatostatin and motilin in pigs. A study of molecular plasma components.
A severe haemorrhage inducing a deep hypovolaemic shock in six anaesthetized pigs effected a plasma somatostatin increase in the superior caval and the portal vein together with a plasma motilin decrease in the portal vein (p less than 0.05). In contrast, the shed blood volume simultaneously instilled intraduodenally in another six anaesthetized pigs in haemodynamic balance induced only an insignificant bimodal somatostatin increase and a modest but significant motilin decrease in plasma from the portal vein (p less than 0.05). Plasma somatostatin immunoreactivity was eluted in four peaks from a Sephadex G-50 Fine column, of which the first peak probably represents somatostatin bound to plasma proteins; the second peak, aggregates of somatostatin-14 and somatostatin degradation products; the third peak, somatostatin-28; and the fourth peak, somatostatin-14. Plasma motilin immunoreactivity eluted in two peaks, of which the first peak possibly represents motilin bound to plasma proteins, and the second peak free plasma motilin. The major changes in portal vein plasma somatostatin and motilin concentrations during the haemorrhagic shock occurred in their free molecular fractions.
Jenssen
T G
TG
Revhaug
A
A
Burhol
P G
PG
Giercksky
K E
KE
Vonen
B
B
D000818:Animals; D001790:Blood Physiological Phenomena; D002850:Chromatography, Gel; D004386:Duodenum / Q000502:physiology*; D005260:Female; D006400:Hematocrit; D006439:Hemodynamics; D006454:Hemoglobins / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D009037:Motilin / Q000097:blood*; D012771:Shock, Hemorrhagic / Q000097:blood*; D013004:Somatostatin / Q000097:blood*; D013552:Swine; D014563:Urodynamics
D006454:Hemoglobins; D013004:Somatostatin; D009037:Motilin