Resonance Raman spectroscopy of complexes of the helix destabilizing proteins GP32 and GP5 with poly(rA) and poly(dA).
The bacteriophage T4 helix destabilizing protein (hdp) gp32 and its complexes with poly(rA) and poly(dA) were studied with ultra-violet resonant Raman spectroscopy. The UV-resonant Raman (UV-RR) spectrum of the complex of gp5, the coat protein of bacteriophage M13, with poly(dA) was also measured and is compared with the spectrum of the gp 32/poly(dA) complex. The excitation wavelength was 245.1 nm. This is on the far UV-side of the first absorption bands of adenine and near a "window" in the protein absorption spectrum. The overlap of fluorescence due to chromophores present in the protein and resonance Raman scattering was prevented by this choice of wavelength. The spectra of the protein/polynucleotide complexes are compared with the native nucleotide spectra measured at varying temperatures. The hyperchromicity which is expected when a nucleotide changes from a stacked to an unstacked conformation was not observed for poly(rA), neither upon temperature increase nor on protein binding. In both cases poly(dA) revealed a clear hyperchromicity. This different behavior of poly(rA) and poly(dA) is probably a consequence of their different conformations. The contributions of the proteins to the spectra is weak except for two bands, at 1550 and 1610 cm-1 due to tryptophan (in case of gp32) and one band near 1610 cm-1 due to tyrosine and phenylalanine.
Otto
C
C
University Twente, Department of Applied Physics, Enschede, The Netherlands.
Chinsky
L
L
Turpin
P Y
PY
de Mul
F F
FF
Harmsen
B J
BJ
Greve
J
J
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004265:DNA Helicases; D004268:DNA-Binding Proteins; D009690:Nucleic Acid Conformation; D011061:Poly A; D011485:Protein Binding; D013059:Spectrum Analysis, Raman; D013604:T-Phages; D013696:Temperature; D014764:Viral Proteins
D004268:DNA-Binding Proteins; D014764:Viral Proteins; C060035:gp32 protein, Enterobacteria phage T4; C027729:helix-destabilizing proteins; D011061:Poly A; C015465:poly(dA); D004265:DNA Helicases