title: Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cDNA clone.
abstract: cDNA clones for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were isolated from rat liver expression libraries in lambda gt11 by antibody, oligonucleotide, and cDNA screening. One 1860 bp long clone contained a full-length nucleotide sequence coding for the 470 amino acids of each of the two identical subunits of the bifunctional enzyme. This clone also contained untranslated sequences, one 173 bp long upstream from the ATG start codon and one 271 bp long downstream from the TGA stop codon. The clone was terminated by a poly(A) tail of 29 nucleotides.
authors.affiliation: Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Gent, Belgium.
authors.forename: M I
authors.initials: MI
authors.lastname: Darville
authors.forename: K M
authors.initials: KM
authors.lastname: Crepin
authors.forename: J
authors.initials: J
authors.lastname: Vandekerckhove
authors.forename: J
authors.initials: J
authors.lastname: Van Damme
authors.forename: J N
authors.initials: JN
authors.lastname: Octave
authors.forename: M H
authors.initials: MH
authors.lastname: Rider
authors.forename: M J
authors.initials: MJ
authors.lastname: Marchand
authors.forename: L
authors.initials: L
authors.lastname: Hue
authors.forename: G G
authors.initials: GG
authors.lastname: Rousseau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D051381:Rats
chemical_list: D004247:DNA; D017853:Phosphotransferases (Alcohol Group Acceptor); D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases