title: Functional comparison of DCCD-sensitive Na+/H+ antiporter in Halobacterium halobium with monensin.
abstract: Membrane vesicles from Halobacterium halobium create a large, inside negative membrane potential (delta psi) and small, inside alkaline pH gradient (delta pH) by illumination in 3 M NaCl. delta psi was the major component of a proton electrochemical potential (delta microH+) over a pH range from 5 to 8. After DCCD treatment of the vesicles, delta psi was replaced by delta pH due to the inhibition of the intrinsic delta pH----delta psi transformation process: delta psi formation in light is markedly retarded and an inversely large delta pH is established at these pHs. DCCD-caused changes in delta psi and delta pH were completely restored to the control level by the addition of monensin, an electroneutral Na+/H+ exchanger. The ratio of DCCD-caused change in delta pH and delta psi was identical to that of monensin-recovered delta psi and delta pH. The delta psi/delta pH ratio was approximately 0.8, that is, 100 mV of delta pH was transformed into 78 mV of delta psi. The present results indicate that the intrinsic activity of the DCCD-sensitive delta pH----delta psi transformation is mediated by an electroneutral Na+/H+ exchange.
authors.affiliation: Department of Radiochemistry-Biology, Niigata College of Pharmacy, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Konishi
mesh_terms: D001693:Biological Transport, Active; D002234:Carbodiimides / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D006217:Halobacterium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008985:Monensin / Q000494:pharmacology*; D011522:Protons; D012964:Sodium / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers
chemical_list: D002234:Carbodiimides; D002352:Carrier Proteins; D011522:Protons; D017923:Sodium-Hydrogen Exchangers; D004024:Dicyclohexylcarbodiimide; D008985:Monensin; D012964:Sodium