[Detection of pre-S gene products in HBsAg carriers and in hepatitis B vaccines].
The immunogenicity and efficacy of different commercially available vaccines against hepatitis B virus (HBV) has been demonstrated in a number of controlled vaccination trials throughout the world. However, the significance of pre-S proteins in hepatitis B vaccines is still controversially discussed. Therefore, a radioimmunoassay (RIA) was developed to detect pre-S proteins in HBsAg-carriers as well as in different hepatitis B vaccines. This study confirmed the high amount of pre-S proteins as well as poly-albumin receptors in purified Dane particles and sera of patients with acute hepatitis B. The currently available plasma derived vaccine H-B-Vax and the recombinant hepatitis B vaccines Gen H-B-Vax and Engerix B yielded negative results in pre-S- and poly-albumin-receptor-RIAs. The plasma derived vaccine Hevac B Pasteur included pre-S encoded proteins. Future vaccination trials in man have to evaluate the role of pre-S antigens and antibodies in the pathogenesis and the protection of hepatitis B infection including recombinant pre-S vaccines which are under development.
Gerken
G
G
I. Medizinische Klinik der Johannes-Gutenberg-Universität Mainz.
Manns
M
M
Kyriatsoulis
A
A
Hess
G
G
Meyer zum Büschenfelde
K H
KH
D002353:Carrier State / Q000276:immunology*; D006509:Hepatitis B / Q000276:immunology*; D006511:Hepatitis B Antigens / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D011498:Protein Precursors / Q000276:immunology*; D011863:Radioimmunoassay; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000032:analysis; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D011498:Protein Precursors; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D014761:Viral Hepatitis Vaccines; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen
Nachweis von Prä-S-Genprodukten bei HBsAg-Trägern und in Hepatitis-B-Vakzinen.