The inhibition of mitochondrial F1-ATPase by 1,5-difluoro-2,4-dinitrobenzene.
1,5-Difluoro-2,4-dinitrobenzene completely inhibits F1-ATPase when it is used at micromolar concentrations and the inhibitor/enzyme molar ratio is equal to 3. The inhibition can be reversed by dithiothreitol treatment. 7-Chloro-4-nitrobenzofurazan treatment of F1-ATPase does not prevent the reaction of the enzyme with 1,5-difluoro-2,4-dinitrobenzene. The 1,5-difluoro-2,4-dinitrobenzene-induced inhibition is thought to be a result of the modification of a tyrosine residue with pK 9.1.
Akhrem
A A
AA
Kisel
M A
MA
Kozlov
I A
IA
Tsybovsky
I S
IS
Vulfson
E N
EN
D009327:4-Chloro-7-nitrobenzofurazan / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology*; D009578:Nitrobenzenes / Q000494:pharmacology*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*
D009578:Nitrobenzenes; D000255:Adenosine Triphosphate; D004139:Dinitrofluorobenzene; D006180:Proton-Translocating ATPases; D009327:4-Chloro-7-nitrobenzofurazan; C002041:1,5-difluoro-2,4-dinitrobenzene; D004229:Dithiothreitol