title: Irreversible inactivation of Saccharomyces cerevisiae fructose-1,6-bisphosphatase independent of protein phosphorylation at Ser11.
abstract: The fructose-1,6-bisphosphatase gene was used with multicopy plasmids to study rapid reversible and irreversible inactivation after addition of glucose to derepressed Saccharomyces cerevisiae cells. Both inactivation systems could inactivate the enzyme, even if 20-fold over-expressed. The putative serine residue, at which fructose-1,6-bisphosphatase is phosphorylated, was changed to an alanine residue without notably affecting the catalytic activity. No rapid reversible inactivation was observed with the mutated enzyme. Nonetheless, the modified enzyme was still irreversibly inactivated, clearly demonstrating that phosphorylation is an independent regulatory circuit that reduces fructose-1,6-bisphosphatase activity within seconds. Furthermore, irreversible glucose inactivation was not triggered by phosphorylation of the enzyme.
authors.affiliation: Medizinisch-Naturwissenschaftliches Forschungszentrum, Universität Tübingen, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Rose
authors.forename: K D
authors.initials: KD
authors.lastname: Entian
authors.forename: L
authors.initials: L
authors.lastname: Hofmann
authors.forename: R F
authors.initials: RF
authors.lastname: Vogel
authors.forename: D
authors.initials: D
authors.lastname: Mecke
mesh_terms: D001483:Base Sequence; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors* / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D012694:Serine
chemical_list: D012694:Serine; D006597:Fructose-Bisphosphatase