The structure of tubes of bovine rotavirus nucleocapsid protein (VP6) assembled in vitro.
The structure of tubes of reassembled nucleocapsid protein (VP6) from bovine rotavirus (BRV) was determined using optical diffraction of electron micrographs. The tubes consist of a five-start helix of hexagons, with 38 hexagons per helix in a true repeat of three turns. The morphological subunits comprising the hexagons are probably elongated trimers. The structure of naturally occurring tubes (D. Chasey and J. Labram, 1983, J. Gen. Virol. 64, 863-872) was also examined and shown to be similar but not identical to that of tubes assembled in vitro. Considerations of the assembly process are discussed.
Ready
K F
KF
Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan, Canada.
Buko
K M
KM
Whippey
P W
PW
Alford
W P
WP
Bancroft
J B
JB
D000818:Animals; D002213:Capsid / Q000032:analysis* / Q000648:ultrastructure; D002417:Cattle; D002460:Cell Line; D003198:Computer Simulation; D008854:Microscopy, Electron; D008956:Models, Chemical; D012401:Rotavirus / Q000032:analysis / Q000648:ultrastructure*; D014461:Ultracentrifugation; D014758:Viral Core Proteins / Q000032:analysis* / Q000648:ultrastructure; D014961:X-Ray Diffraction
D014758:Viral Core Proteins