Immunochromatography and cardiotoxicity of sea nettle (Chrysaora quinquecirrha) polyps and cysts.
The cardiotoxicity and polypeptide content of sea nettle (Chrysaora quinquecirrha) polyps and cysts were studied. Crude polyp preparations were lethal to mice. Both crude polyp and cyst preparations were toxic to embryonic chick cardiocytes. The polyp cardiotoxin factor was purified ten-fold by immunosorbent chromatography using anti-sea nettle or anti-man-o'war (Physalia physalis) monoclonal antibodies. Even though the polyps were incubated at a constant temperature, it appeared that there was an inverse relationship between the presence of proteins of 160,000 and 55,000 mol. wt as winter progressed.
Olson
C E
CE
Cargo
D G
DG
Calton
G J
GJ
Burnett
J W
JW
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002642:Chick Embryo; D002845:Chromatography / Q000379:methods; D003064:Cnidarian Venoms / Q000032:analysis / Q000276:immunology / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D006321:Heart / Q000187:drug effects* / Q000196:embryology; D007120:Immunochemistry; D051379:Mice; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms; C031441:sea nettle toxin