Difference in the in vitro metabolism of leukotrienes in the exudates from allergic and nonallergic rat pleurisies.
The metabolism of leukotrienes (LTs) in the cell-containing inflammatory exudate of rat pleurisy was studied in vitro. The exudates of both nonallergic carrageenin-induced pleurisy and IgG immune complex-mediated pleurisy converted 3H-LTB4 to 20-OH LTB4, but virtually did not metabolized 3H-LTC4 or 3H-LTE4 up to 2 hrs. 3H-LTD4 was changed to LTC4 by the exudate of non-allergic pleurisy, whereas 3H-LTD4 was metabolized to LTE4 by that of allergic pleurisy. Reflecting on the different metabolism, the gamma-glutamyl transpeptidase activity in the exudate of carrageenin-induced pleurisy was significantly higher than that in IgG immune complex mediated pleurisy. The enzyme activity was not derived from the blood itself, but from the infiltrated polymorphonuclear leukocytes. The activity of the cell homogenate in both exudates was not significantly different. Thus, it could be concluded that the difference in the metabolism of LTD4 between the nonallergic and allergic pleural exudates in vitro was mainly attributable to the enhanced activity of the gamma-glutamyl transpeptidase released in the exudate.
Ueno
A
A
Tanaka
K
K
Katori
M
M
D000818:Animals; D000936:Antigen-Antibody Complex; D002351:Carrageenan; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D007249:Inflammation / Q000378:metabolism; D007700:Kinetics; D007975:Leukotriene B4 / Q000378:metabolism*; D008297:Male; D010998:Pleurisy / Q000209:etiology / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D013189:SRS-A; D007975:Leukotriene B4; D002351:Carrageenan; D005723:gamma-Glutamyltransferase