Reversed-phase liquid chromatography of the twenty-two tetrachlorodibenzo-p-dioxin isomers on pyrenylethyl- and octadecylsilylated silica gel columns.
To purify the tetrachlorobenzo-p-dioxins synthesized for chemical and biological reference standards, reversed-phase liquid chromatography on pyrenylethyl- and octadecylsilylated stationary phase (PYE and C18) columns was employed with 100% methanol. The pyrenylethyl phase satisfactorily separated isomers resulting from mixtures of reaction products which had not been adequately separated with conventional C18 or silica gel. The use of a single chromatographic mode, liquid chromatography, and a single mobile phase with columns of distinctly different properties, C18 and PYE, separates 20 of the 22 isomers from each other and from the 1246/1249 pair. (The observed properties of the two columns afford insight into steric and polarizability differences among isomers.)
Barnhart
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ER
Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333.
Patterson
D G
DG
Tanaka
N
N
Araki
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004147:Dioxins / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D000072317:Polychlorinated Dibenzodioxins / Q000302:isolation & purification*; D012822:Silicon Dioxide
D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D012822:Silicon Dioxide