title: [The use of monoclonal antibodies for purification of ribonuclease H from E. coli].
abstract: Monoclonal antibodies to E. coli ribonuclease H were obtained from two hybrid clones. Using the monoclonal antibodies two immunosorbents were synthesized for RNase H which have a slight difference in the capacity and do not differ in the conditions of antigen elution. A homogeneous (according to electrophoresis in PAAG) preparation of the enzyme was obtained using the synthesized immunosorbents.
authors.forename: V V
authors.initials: VV
authors.lastname: Smolianinov
authors.forename: A P
authors.initials: AP
authors.lastname: Kaliuzhnaia
authors.forename: G V
authors.initials: GV
authors.lastname: Shekhvatova
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Mantsygin
authors.forename: N V
authors.initials: NV
authors.lastname: Sviatukhina
mesh_terms: D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology*; D007163:Immunosorbent Techniques; D016914:Ribonuclease H
chemical_list: D000911:Antibodies, Monoclonal; D004722:Endoribonucleases; D016914:Ribonuclease H
vernacular_title: Ispol'zovanie monoklonal'nykh antitel dlia ochistki ribonukleazy H E. coli.