title: [Isolated human gastric mucosa cells--studies on physiologic and pharmacologic regulatory mechanisms].
abstract: Cells were isolated by use of collagenase, EDTA and pronase form human gastric mucosa obtained at peptic ulcer surgery (n = 61) or at Whipple's operations (n = 6). Enriched parietal cell fractions were prepared by isopycnic centrifugation with Percoll. H+ production, intracellular instrinsic factor and histamine content were maximal in the low density fraction containing 75% parietal cells and--among other nonparietal cell types--mast cells. H+ production, intrinsic factor secretion and adenylate cyclase-activity responded to histamine stimulation in a concentration dependent manner. Response was blocked by histamine H2 receptor antagonists (rantidine, famotidine). Dibutyryl cAMP and the phosphodiesterase inhibitor IMX were the most powerful stimuli whereas carbachol, hexoprenaline and pentagastrin were less effective. Prostaglandin E2 and 6-keto-PGF2 alpha occurred in the highest concentrations in the low density cell fraction. PG production increased linearly for 15 min and seemed to be influenced by the intracellular calcium level.
authors.forename: W
authors.initials: W
authors.lastname: Schepp
authors.forename: S E
authors.initials: SE
authors.lastname: Miederer
authors.forename: H J
authors.initials: HJ
authors.lastname: Ruoff
authors.forename: U
authors.initials: U
authors.lastname: Wulfhekel
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000328:Adult; D000368:Aged; D000632:Aminopyrine / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015738:Famotidine; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology*; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006801:Humans; D007437:Intrinsic Factor / Q000378:metabolism; D008854:Microscopy, Electron; D008875:Middle Aged; D010295:Parietal Cells, Gastric / Q000166:cytology; D011453:Prostaglandins / Q000378:metabolism; D011899:Ranitidine / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
chemical_list: D006635:Histamine H2 Antagonists; D011453:Prostaglandins; D013844:Thiazoles; D000632:Aminopyrine; D015738:Famotidine; D006632:Histamine; D011899:Ranitidine; D007437:Intrinsic Factor; D000242:Cyclic AMP; D000262:Adenylyl Cyclases
vernacular_title: Isolierte menschliche Magenschleimhautzellen--Untersuchungen physiologischer und pharmakologischer Regulationsmechanismen.