Induction of liver glycogenolysis by phenylephrine: comparison between superfusion and perfusion techniques.
Superfusion of liver tissue slices was performed by a technique specially developed for that purpose, and compared to the whole organ perfusion technique of Jakob and Diem in order to assess the merits of the former technique. Activation of liver glycogenolysis of phenylephrine has been examined by both techniques. Both techniques showed important similarities and some dissimilarities. It was concluded that the application of superfusion techniques requires careful quantitative assessment of each experimental detail for each physiological event examined.
Leskovac
V
V
Trivić
S
S
Kalco
M
M
D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008112:Liver Glycogen / Q000378:metabolism*; D008297:Male; D051379:Mice; D010477:Perfusion; D010656:Phenylephrine / Q000008:administration & dosage*
D008112:Liver Glycogen; D010656:Phenylephrine; D005947:Glucose