In-vitro cytotoxic activity of cross-linked protein microcapsules.
Microcapsules (5-100 microns) were prepared through interfacial cross-linking of various proteins (human serum albumin, lysozyme, haemoglobin, casein, pepsin) with glutaraldehyde or terephthaloylchloride. Surprisingly they all showed an inhibitory effect on cultured cells in a concentration range of 100 micrograms ml-1 to 10 mg ml-1. This effect seemed non-specific, reversible and to depend on contact with the cell plasma membrane. The electric charges of microcapsules could be involved in the inhibition phenomenon.
Desoize
B
B
Jardillier
J C
JC
Kanoun
K
K
Guerin
D
D
Levy
M C
MC
D000818:Animals; D002214:Capsules / Q000009:adverse effects* / Q000032:analysis; D002364:Caseins / Q000009:adverse effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D005079:Excipients / Q000633:toxicity*; D005976:Glutaral / Q000009:adverse effects; D006454:Hemoglobins / Q000009:adverse effects; D006801:Humans; D051379:Mice; D009113:Muramidase / Q000009:adverse effects; D010434:Pepsin A / Q000009:adverse effects; D012709:Serum Albumin / Q000009:adverse effects
D002214:Capsules; D002364:Caseins; D005079:Excipients; D006454:Hemoglobins; D012709:Serum Albumin; D009113:Muramidase; D010434:Pepsin A; D005976:Glutaral