title: Control of early gene expression in Dictyostelium.
abstract: We have examined the expression of a cAMP pulse-repressed and two cAMP pulse-induced genes in response to cAMP and caffeine under a number of different physiological conditions, and in several classes of development mutants altered in cAMP-mediated signal transduction pathways. The data presented help characterize the mutants with regard to early gene expression. Analysis of the data indicates that full induction of the pulse-induced or repression of the pulse-repressed genes requires cycles of activation and adaptation of the cAMP receptor but does not require a rise in intracellular cAMP. Comparison of the results obtained between different mutant classes suggests that repression and activation of the two classes of genes can be uncoupled, implying that different intracellular mechanisms control these processes. In addition, we examined the effects of caffeine and show that it can induce pulse-induced mRNA accumulation in the absence of cAMP.
authors.affiliation: Department of Biology, University of California, San Diego, La Jolla 92093.
authors.forename: S K
authors.initials: SK
authors.lastname: Mann
authors.forename: C
authors.initials: C
authors.lastname: Pinko
authors.forename: R A
authors.initials: RA
authors.lastname: Firtel
mesh_terms: D002110:Caffeine / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004023:Dictyostelium / Q000187:drug effects / Q000235:genetics*; D005786:Gene Expression Regulation; D005800:Genes, Fungal / Q000187:drug effects; D009154:Mutation
chemical_list: D002110:Caffeine; D000242:Cyclic AMP