Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.
The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.
Cotecchia
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S
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Schwinn
D A
DA
Randall
R R
RR
Lefkowitz
R J
RJ
Caron
M G
MG
Kobilka
B K
BK
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000032:analysis*; D019204:GTP-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010716:Phosphatidylinositols / Q000378:metabolism; D011942:Receptors, Adrenergic, alpha / Q000235:genetics*
D010716:Phosphatidylinositols; D011942:Receptors, Adrenergic, alpha; D004247:DNA; D019204:GTP-Binding Proteins