Insertion sequence IS2 in the cyanobacterium Chlorogloeopsis fritschii.
A cloned DNA fragment, previously demonstrated to encode ribulose bisphosphate carboxylase/oxygenase (RuBisCO) of Chlorogloeopsis fritschii strain CCAP1411/1b, is shown also to include the entire transposable element, IS2, normally a resident in the Escherichia coli genome. Southern-blot hybridisation experiments confirm the presence of IS2 in the C. fritschii genome. This finding adds a new and unrelated species to the known host range of this element and provides evidence of genetic transfer between the Gram-negative E. coli and cyanobacteria. This may also have significance in relation to the nucleotide sequence rearrangements known to occur adjacent to RuBisCO and nif genes in other nitrogen-fixing cyanobacteria.
Machray
G C
GC
Department of Biological Sciences, University of Dundee, U.K.
Vakeria
D
D
Codd
G A
GA
Stewart
W D
WD
D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D004251:DNA Transposable Elements; D004247:DNA