title: The responsiveness of human papillomavirus upstream regulatory regions to herpes simplex virus immediate early proteins.
abstract: We have tested the responsiveness of the upstream regulatory regions (URR) of human papillomaviruses (HPV) to transactivation by the herpes simplex virus type 1 (HSV-1) immediate early proteins ICP4 (Vmw175) and ICP0 (Vmw110), using recombinant plasmids which contain URRs of HPV 1, 11 or 16 upstream of an SV40-promoted CAT gene. Transfection of the HPV-CAT plasmids into mouse cells expressing a temperature-sensitive ICP4 protein enabled us to demonstrate that ICP4 enhances transcription of the HPV 1 and 16 URRs, but not of the HPV 11 URR. Cotransfection of the HPV CAT plasmids with a plasmid encoding ICP0 indicated that only the URR of HPV 16 responded to this protein, and that the response was host cell dependent.
authors.affiliation: Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: C T
authors.initials: CT
authors.lastname: McCusker
authors.forename: S
authors.initials: S
authors.lastname: Bacchetti
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D017874:Immediate-Early Proteins; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D012045:Regulatory Sequences, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic; D014162:Transfection; D044767:Ubiquitin-Protein Ligases; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: C050678:IE1 protein, Human herpesvirus 1; D017874:Immediate-Early Proteins; D014764:Viral Proteins; C048925:herpes simplex virus, type 1 protein ICP4; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1