Activation specificity of commonly employed mitogens for canine B- and T-lymphocytes.
Six day in vitro cultures of canine mononuclear leukocytes with or without prior carbonyl iron depletion were established with 5 commonly employed mitogens. Cultures were assessed for B-cell differentiation by induction of cytoplasmic and supernatant released IgG, M and A and T-cell differentiation by expression of Thy-1 antigen on the cell surface of lymphoblasts. The mitogen concanavalin A was shown to be a restricted T-cell mitogen, whereas pokeweed mitogen and protein A from Staphylococcus aureus stimulated maximal B-cell differentiation. These data establish the mitogenic specificity for canine lymphocytes in unfractionated peripheral blood leukocyte cultures and will permit an in vitro evaluation of various substances upon T- and B-lymphocyte functions.
Krakowka
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Ringler
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D000818:Animals; D000917:Antibody Formation; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004261:DNA Replication; D004285:Dogs; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D037121:Plant Lectins; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013205:Staphylococcal Protein A / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D037102:Lectins; D008934:Mitogens; D010835:Phytohemagglutinins; D037121:Plant Lectins; D011043:Pokeweed Mitogens; D013205:Staphylococcal Protein A; D018800:Thy-1 Antigens; C033619:lentil lectin; D003208:Concanavalin A