title: [mRNA localization and content in bovine kininogen].
abstract: The bradykinin (BK) gene chemically synthesized and cloned into pBR 322 was used for the study of tissue localization and quantitation of mRNA coding for the BK precursor, kininogen. Poly(A+)-mRNAs from bovine liver, spleen, kidney, mammary gland and pancreas were used for dot-hybridization to [32P]DNA of the BK gene or to the plasmid-containing BK gene. The experimental results demonstrate that [32P]DNA of BK is hybridized only to the liver poly(A+)-RNA, which proves the liver to be the main kininogen mRNA-producing tissue. In other tissues, the kininogen mRNA synthesis is either altogether absent or its level is two orders of magnitude less as compared to the liver. Several approaches for the quantitation of the kininogen mRNA were developed. The amount of this mRNA was shown to be about 0.6% of the total cellular poly(A+)-RNA. Poly(A+)-RNA which is bound to BK DNA-cellulose and is enriched with BK-coding sequences was used for the study of the hybridization kinetics and translation in a cell-free system from rabbit reticulocytes. The polypeptides synthesized contain BK as was shown by the use of a bio-test in rat uterus.
authors.forename: G E
authors.initials: GE
authors.lastname: Sulimova
authors.forename: A A
authors.initials: AA
authors.lastname: Akhundova
authors.forename: T V
authors.initials: TV
authors.lastname: Kapelinskaia
authors.forename: S I
authors.initials: SI
authors.lastname: Gorodetskiĭ
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000235:genetics / Q000494:pharmacology; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005260:Female; D005796:Genes; D066298:In Vitro Techniques; D007704:Kininogens / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution; D014590:Uterine Contraction / Q000187:drug effects
chemical_list: D007704:Kininogens; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA; D001920:Bradykinin
vernacular_title: Lokalizatsiia mesta sinteza i soderzhanie mRNK kininogena byka.