Excitatory amino acid receptors in hippocampal neurons: kainate fails to desensitize them.
The excitatory responses to L-glutamate (L-Glu), quisqualate (QA) and kainate (KA) have been investigated in isolated pyramidal cells from rat hippocampus using intracellular perfusion and concentration clamp techniques. The responses to L-Glu and QA demonstrated rapid desensitization and complete cross-desensitization, while KA produced a non-desensitizing response. The activation of KA response was determined by the level of desensitization induced by L-Glu or QA pretreatment. It is concluded that QA, KA and L-Glu activate the same excitatory receptors with apparent Kd values of 9.3 X 10(-5) M, 5.0 X 10(-4) M and 1.1 X 10(-3) M, respectively.
Kiskin
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NI
Krishtal
O A
OA
Tsyndrenko AYa
D000818:Animals; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology*; D009474:Neurons / Q000378:metabolism*; D010069:Oxadiazoles / Q000494:pharmacology; D016318:Quisqualic Acid; D051381:Rats; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000187:drug effects*
D005971:Glutamates; D010069:Oxadiazoles; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D018698:Glutamic Acid; D016318:Quisqualic Acid; D007608:Kainic Acid