title: Presence of phosphatidylcholine hydroperoxide in human plasma.
abstract: A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cytochrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2,000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.
authors.affiliation: Department of Food Chemistry, Faculty of Agriculture, Tohoku University, Miyagi.
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: K
authors.initials: K
authors.lastname: Yasuda
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
authors.forename: T
authors.initials: T
authors.lastname: Kaneda
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003574:Cytochrome c Group; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000097:blood*
chemical_list: D003574:Cytochrome c Group; D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide; D008165:Luminol