title: Isolation and primary structures of elephant adrenocorticotropin and beta-lipotropin.
abstract: Adrenocorticotropin and beta-lipotropin have been isolated and purified from elephant pituitary glands. The primary structures of these two hormones were determined by amino acid and sequence analyses of enzymatically cleaved peptides from the hormones. Peptide purification involved the use of gel filtration, reverse phase high performance liquid chromatography, and paper electrophoresis.
authors.affiliation: Laboratory of Molecular Endocrinology, University of California, San Francisco.
authors.forename: C H
authors.initials: CH
authors.lastname: Li
authors.forename: M M
authors.initials: MM
authors.lastname: Oosthuizen
authors.forename: D
authors.initials: D
authors.lastname: Chung
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004606:Elephants / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D010902:Pituitary Gland / Q000032:analysis*; D013045:Species Specificity; D014357:Trypsin; D008083:beta-Lipotropin / Q000302:isolation & purification*
chemical_list: D010446:Peptide Fragments; D000324:Adrenocorticotropic Hormone; D008083:beta-Lipotropin; D014357:Trypsin