title: Effect of topical fluoride treatments on fluoride distribution during in vitro caries-like lesion formation.
abstract: Tooth sections were treated in vitro for 4 min with APF, SnF2, or received no treatment. Each treatment group then received washes of KOH, (24 h), an inorganic solution (24 h), or DDH2O (2 min) and were placed into dialyzed 15% w/v gel (pH 4.3) containing 0.15 mM hydroxyapatite, but no fluoride (less than 0.02 ppm F). Adjacent nontreated sound enamel acted as the control. Lesion microdissection, after 2 weeks exposure to the acidified gel, revealed an inverse relationship between lesion body fluoride concentration and lesion depth. The lesion depth was smallest, and the lesion body fluoride concentration was greatest in both fluoride-treated groups after a 2-min DDH2O wash, a 24-hour inorganic wash, and a 24-hour KOH wash, respectively. These data support the theory that the progress of carious lesions is related to the fluoride concentration in the lesion and that the fluoride concentration in the lesion is related to the acquired fluoride concentration in sound enamel.
authors.affiliation: School of Dental Medicine, Department of Pediatric Dentistry, University of Connecticut, Farmington.
authors.forename: B H
authors.initials: BH
authors.lastname: Clarkson
authors.forename: S E
authors.initials: SE
authors.lastname: Hansen
authors.forename: J S
authors.initials: JS
authors.lastname: Wefel
mesh_terms: D000149:Acidulated Phosphate Fluoride / Q000494:pharmacology; D001639:Bicarbonates / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D003731:Dental Caries / Q000209:etiology* / Q000378:metabolism; D003743:Dental Enamel / Q000032:analysis / Q000378:metabolism*; D005459:Fluorides / Q000032:analysis / Q000493:pharmacokinetics*; D005460:Fluorides, Topical / Q000494:pharmacology*; D006801:Humans; D006878:Hydroxides / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds; D012964:Sodium / Q000494:pharmacology; D017693:Sodium Bicarbonate; D012969:Sodium Fluoride / Q000494:pharmacology
chemical_list: D001639:Bicarbonates; D005460:Fluorides, Topical; D006878:Hydroxides; D010710:Phosphates; D017680:Potassium Compounds; D000149:Acidulated Phosphate Fluoride; D017693:Sodium Bicarbonate; D012969:Sodium Fluoride; D012964:Sodium; D002122:Calcium Chloride; D005459:Fluorides; D011188:Potassium; C018279:sodium phosphate; C029943:potassium hydroxide