Analysis of antigenically intermediate strains of subgenus B and D adenoviruses from AIDS patients.
We carried out detailed antigenic and restriction enzyme (RE) analyses on the subgenus B and D adenovirus isolates from 48 AIDS patients. These isolates were unusual both in the diversity of serotypes and in the number of intermediate strains identified. All unusual isolates were strain-purified and tested by serum neutralization (SN) and hemagglutination-inhibition (HI) tests with reference horse and rabbit antisera to all the prototype human adenoviruses; conversely, rabbit antisera were prepared to 16 selected strains from this study and tested by SN and HI with all prototype viruses. Among subgenus B strains, 6 DNA variants of Ad 11 isolates were distinguished by endonucleases BamHI, BglII, BstEII, HindIII, and SmaI. The D 2 variant of Ad 11 was prominent with 5 isolates, and 7 other isolates differed from D 2 in only 1 or 2 enzymes. HindIII was the most discriminative endonuclease for Ad 11 and related strains. Within subgenus D, there were 16 isolates of intermediate strains, including 4 intermediate types related to new Ad types 43-47. The RE patterns of subgenus D strains showed fragment distributions typical of subgenus D, with various unique patterns. Particular care was taken to analyze multiple strains from the same patient, recovered as much as 8 months apart, for evidence of genetic changes. The possible long-term infections with these viruses may provide the opportunity for mutations and recombination to occur, with the resultant generation of a variety of new strains of adenoviruses.
Hierholzer
J C
JC
Respiratory and Enteric Viruses Branch, Centers for Disease Control, Atlanta, Georgia.
Adrian
T
T
Anderson
L J
LJ
Wigand
R
R
Gold
J W
JW
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000258:Adenovirus Infections, Human / Q000150:complications; D000260:Adenoviruses, Human / Q000145:classification / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D004262:DNA Restriction Enzymes; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D009500:Neutralization Tests; D011817:Rabbits; D012703:Serotyping
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004262:DNA Restriction Enzymes