Reduction and disappearance of anomalous fluorescence patterns by means of absorption of HIV and EBV positive sera to guinea pig kidney and calf erythrocytes: a preliminary study.
Anomalous pictures observed in HIV infected H9 line cells by immunofluorescence technique, were investigated on sera of drug addicts and on controls. Strong fluorescence artifacts were markedly reduced after an overnight incubation at 4 degrees C. A complete disappearance was obtained after absorption to guinea pig kidney and calf erythrocytes of anti EBV positive sera. This result was fully independent of any anti HIV positivity.
Lambert-Gardini
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Centro Geriatrico Nomentano, Tor Lupara, Roma.
Colarossi
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GG
Sergiacomi
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Gozzo
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L
Nuzzolo
L
L
D015492:AIDS Serodiagnosis; D000327:Adsorption; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D002417:Cattle; D002460:Cell Line; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology*; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000150:complications / Q000175:diagnosis*; D006509:Hepatitis B / Q000150:complications / Q000175:diagnosis; D006510:Hepatitis B Antibodies / Q000097:blood; D006566:Herpesviridae Infections / Q000150:complications / Q000175:diagnosis; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000276:immunology*; D015819:Substance Abuse, Intravenous / Q000097:blood / Q000150:complications
D000914:Antibodies, Viral; D015483:HIV Antibodies; D006510:Hepatitis B Antibodies