Interferon-gamma requires serum retinoids to promote the expression of tissue transglutaminase in cultured human blood monocytes.
The culture of HPBM in serum-containing medium induced a large accumulation of the protein cross-linking enzyme, tissue TGase. Immune IFN enhanced the expression of tissue TGase in cultured monocytes. Enzyme-inducing activity, both in normal and IFN-treated cells, was completely blocked by depleting the serum of the lipid fraction. The readdition of retinol at a physiologic concentration (1 micron) to delipidized serum completely restored the enzyme-inducing activity in cultured monocytes. Thus, serum retinoids seem to play an important regulatory role in the expression of tissue TGase gene in differentiating human monocytes.
Mehta
K
K
Lopez-Berestein
G
G
Moore
W T
WT
Davies
P J
PJ
D000217:Acyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D002478:Cells, Cultured; D004790:Enzyme Induction; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology*; D009000:Monocytes / Q000201:enzymology*; D012176:Retinoids / Q000097:blood / Q000502:physiology*; D014018:Tissue Distribution; D011503:Transglutaminases
D012176:Retinoids; D007371:Interferon-gamma; D000217:Acyltransferases; D011503:Transglutaminases