Bovine parainfluenza-3 virus proteins recognized by antibodies of aerosol-exposed calves.
Bovine parainfluenza-3 (BPI-3) virus proteins were radiolabeled with [35S]methionine. Control serum containing antibodies to BPI-3 virus obtained from calves that had been immunized by aerosol exposure and by IM inoculation and sera of cattle that were exposed to BPI-3 virus by aerosol only were used to immunoprecipitate BPI-3 virus proteins. The immunoprecipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Viral proteins with estimated molecular weights of 72,000, 70,000, and 58,000 were recognized by the control serum and the sera from calves exposed to BPI-3 virus only by aerosol. These proteins were the hemagglutinin/neuraminidase, nucleoprotein, and fusion protein. The results indicated that a single aerosol exposure to BPI-3 virus induces antibodies to the same BPI-3 virus proteins as do combined aerosol and parenteral inoculations with the virus.
Toth
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Department of Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Frank
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D000336:Aerosols; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006385:Hemagglutination Inhibition Tests; D007106:Immune Sera / Q000276:immunology; D009500:Neutralization Tests; D010224:Parainfluenza Virus 3, Human / Q000276:immunology*; D011233:Precipitin Tests; D018108:Respirovirus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000336:Aerosols; D000914:Antibodies, Viral; D007106:Immune Sera; D014764:Viral Proteins