The effect of restriction enzyme digestion of human metaphase chromosomes on C-band variants of chromosomes 1 and 9.
Human metaphase chromosomes, fixed on slides, have beent treated with 8 different restriction endonucleases and 29 combinations of 2 restriction enzymes prior to staining with Giemsa. The endonucleases AluI and DdeI and the combinations AluI + DdeI, AluI + HaeIII, AluI + HinfI, and AluI + MboI have then been used to digest metaphase chromosomes of nine individuals with C-band variants of chromosomes 1 or 9, obtained by the CBG technique. The restriction enzyme resistant chromatin of the paracentromeric regions of chromosomes 1 and 9 has been measured and compared with the corresponding CBG-bands. The size of the enzyme resistant chromatin regions depend upon the type of enzyme(s) used. Treatment with AluI + MboI was the only digestion that acted differently on different chromosome pairs. However, within one pair of homologous chromosomes, all digestions revealed the same variations as conventional C-banding.
Hedemann
U
U
Institut für Humangenetik der Medizinischen Universität zu Lübeck, Federal Republic of Germany.
Schürmann
M
M
Schwinger
E
E
D002871:Chromosome Banding / Q000379:methods*; D002878:Chromosomes, Human, Pair 1; D002885:Chromosomes, Human, Pair 16; D002899:Chromosomes, Human, Pair 9; D004262:DNA Restriction Enzymes; D014644:Genetic Variation; D006801:Humans; D008677:Metaphase
D004262:DNA Restriction Enzymes