Effects of tricyclohexylhydroxytin on the kinetics of adenosine triphosphatase system and protection by thiol reagents.
Tricyclohexylhydroxytin, commonly known as Plictran, inhibited Na+, K+-ATPase activity of rat brain synaptosomes in a concentration-dependent manner with median inhibitory concentration (IC-50) of 2 microM. Both K+-stimulated para-nitrophenylphosphatase and [3-H]-ouabain binding to synaptosomes were also inhibited by Plictran with IC-50 values of 11 and 30 microM, respectively. Altered pH and Na+, K+-ATPase activity curves demonstrated comparable inhibition in buffered neutral and alkaline pH ranges, and no inhibition was observed in acidic pH. The inhibition of Na+, K+-ATPase was independent of temperature. Kinetic studies of substrate (ATP) activation of Na+, K+-ATPase indicated uncompetitive inhibition. Results also showed noncompetitive inhibition for p-nitrophenylphosphate and uncompetitive inhibition for K+ activations of p-nitrophenylphosphatase. Preincubation of synaptosomes with dithiothreitol, a sulfhydryl (SH) agent, resulted in the complete protection of Plictran inhibition of Na+, K+-ATPase, K+-para-nitrophenylphosphatase, and [3-H]-ouabain binding. The protection was specific and concentration dependent since cysteine and glutathione did not afford protection. These results indicate that Plictran inhibited Na+, K+-ATPase by interacting with dephosphorylation of the enzyme-phosphoryl complex and exerted a similar effect to that of SH-blocking agents.
Rao
K S
KS
Department of Neurology, University of Mississippi Medical Center, Jackson 39216-4505.
Chetty
S C
SC
Desaiah
D
D
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects* / Q000201:enzymology; D013696:Temperature; D014220:Trialkyltin Compounds / Q000037:antagonists & inhibitors / Q000633:toxicity*
D013438:Sulfhydryl Compounds; D014220:Trialkyltin Compounds; C001765:cyhexatin; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase