CTP-dependent lipid kinases of yeast.
Membrane fractions from yeast Saccharomyces cerevisiae catalyzed a transfer of gamma-phosphate from [gamma-32P]CTP into membranous lipids. Phosphorylated compounds were identified as phosphatidic acid and dolichyl phosphate (DolP). The membrane fraction also catalyzed phosphorylation of the exogenous dolichol. The activity of the phosphorylating enzymes could be modified by the yeast growing conditions; i.e., the enzyme from yeast grown aerobically favored the synthesis of phosphatidate over dolichyl phosphate in the ratio of 3:1, whereas the membrane fraction from anaerobically grown yeast synthesized PA and DolP in the ratio of 0.5:1. The activity of the phosphorylating enzymes could also be modified by divalent cations and the concentration of detergents. Phosphorylation of lipids does not occur in the presence of [gamma-32P]ATP and is not influenced by the presence of UTP or GTP. This result points to the specific role of CTP as a gamma-phosphate donor for the synthesis of phosphatidate and dolichyl phosphates in the yeast system.
Szkopińska
A
A
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
Nowak
L
L
Swiezewska
E
E
Palamarczyk
G
G
D003570:Cytidine Triphosphate / Q000378:metabolism*; D003597:Cytosine Nucleotides / Q000378:metabolism*; D003902:Detergents; D004075:Diglycerides / Q000378:metabolism; D004288:Dolichol Phosphates / Q000096:biosynthesis; D050356:Lipid Metabolism; D008566:Membranes / Q000378:metabolism; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis; D010770:Phosphotransferases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity; D015032:Zinc / Q000494:pharmacology
D003597:Cytosine Nucleotides; D003902:Detergents; D004075:Diglycerides; D004288:Dolichol Phosphates; D010712:Phosphatidic Acids; D010743:Phospholipids; C028306:dolichol monophosphate; D003570:Cytidine Triphosphate; D010770:Phosphotransferases; D015032:Zinc