title: Mutual competitive inhibition between target sites during the restriction endonuclease digestion of DNA.
abstract: The reaction rate of restriction endonuclease was evaluated theoretically, considering the competition between target sites and a nonspecific DNA. An equation for the initial cleavage rate at a single site for a DNA substrate containing more than one recognition site was derived. The consequences for the study of preferential cleavage were discussed.
authors.affiliation: Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
authors.forename: P
authors.initials: P
authors.lastname: Karlovský
mesh_terms: D001667:Binding, Competitive; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes