title: Changes in the number of GnRH-receptive cells during the rat estrous cycle: biphasic effects of estradiol.
abstract: The number of gonadotropin-releasing hormone (GnRH) receptors is known to vary throughout the estrous cycle and in other endocrine states in the rat. These changes in receptors parallel closely the concentrations of serum estradiol during the cycle. In the present study, we used two different cytochemical techniques to determine if changes in GnRH receptors represented alterations in the number of GnRH-receptive cells. Furthermore, we tested the effects of estradiol pretreatment on this phenomenon. Dispersed pituitary monolayers taken at different stages of the cycle were stimulated for 3 min with 1 nM biotinylated [D-lys6]-GnRH (bio-GnRH) which was localized using the avidin-biotin-peroxidase complex (ABC) technique and a black peroxidase substrate. Parallel groups were stained, while living, with an avidin-fluorescein conjugate. Some monolayers were pretreated with physiological concentrations of estradiol benzoate (1 nM-1 pM) prior to bio-GnRH exposure and ABC stains. The resulting stains demonstrated that the percentage of bio-GnRH-receptive cells was 2-3 times greater at 10.00 h proestrus (20.2 +/- 4%) when compared to the same time in estrus (8.7 +/- 2%), diestrus I (5.2 +/- 0.5%), diestrus II (7.6 +/- 1%), and at 17.00 h diestrus II (11.4 +/- 0.9%) and proestrus (7.4 +/- 0.8%). These data correlated well with those obtained from living gonadotropes stained with avidin-fluorescein. Estradiol exerted a biphasic effect dependent upon the stage of the cycle at which the cells were taken.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy and Neurosciences, University of Texas Medical Branch, Galveston.
authors.forename: J M
authors.initials: JM
authors.lastname: Lloyd
authors.forename: G V
authors.initials: GV
authors.lastname: Childs
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004031:Diestrus; D004958:Estradiol / Q000494:pharmacology*; D004971:Estrus; D005260:Female; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011359:Proestrus; D051381:Rats; D011919:Rats, Inbred Strains; D011966:Receptors, LHRH / Q000187:drug effects / Q000378:metabolism*; D012016:Reference Values
chemical_list: D011966:Receptors, LHRH; D004958:Estradiol