title: Cloning and characterization of a genomic probe for malignant catarrhal fever virus.
abstract: A genomic probe specific for malignant catarrhal fever (MCF) virus was cloned by using purified viral DNA from MCF-virus strain WCll. Restriction endonuclease analysis of the purified viral DNA was used to identify the cloned viral genomic fragment. Dot blot hybridization by use of the genomic probe (pRP-5) indicated that the probe hybridized specifically with WCll-MCF virus, as well as with one other isolate of MCF-associated herpesvirus. Hybridization also was observed to a non-MCF virus strain of bovine herpesvirus.
authors.affiliation: Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: L M
authors.initials: LM
authors.lastname: Shih
authors.forename: J M
authors.initials: JM
authors.lastname: Irving
authors.forename: Y C
authors.initials: YC
authors.lastname: Zee
authors.forename: R F
authors.initials: RF
authors.lastname: Pritchett
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D008304:Malignant Catarrh / Q000382:microbiology*; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D004262:DNA Restriction Enzymes