Inactivation of type MM phosphoglycerate mutase by sulfhydryl group reagents during facial embryogenesis.
Type MM phosphoglycerate mutase from free dissected mandibular processes from embryonic rats was reversibly inactivated by tetrathionate, p-chloromercuribenzoate, and Hg2+. Titration with p-chloromercuribenzoate showed the existence of two sulfhydryl groups per enzyme subunit, the modification of which produced a progressive decline in enzyme activity. The apparent Km values for substrate and cofactor were not affected by tetrathionate treatment. Phosphoglycerate mutase inactivated by tetrathionate and by p-chloromercuribenzoate was unable to form the functionally active phosphorylenzyme when mixed with glycerate-2,3-P2. Glycerate-2,3-P2 protected against tetrathionate but failed to protect against Hg2+ and p-chloromercuribenzoate.
Granström
G
G
Department of Histology, University of Gothenburg, Sweden.
Mångs
H
H
D000818:Animals; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004622:Embryo, Mammalian; D005145:Face / Q000196:embryology*; D005147:Facial Bones / Q000196:embryology / Q000201:enzymology; D005152:Facial Muscles / Q000196:embryology / Q000201:enzymology; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D010736:Phosphoglycerate Mutase / Q000037:antagonists & inhibitors*; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D013776:Tetrathionic Acid / Q000494:pharmacology
D002729:Chloromercuribenzoates; D007527:Isoenzymes; D013438:Sulfhydryl Compounds; D013776:Tetrathionic Acid; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase