DNA damage by oxygen radicals and excited state species: a comparative study using enzymatic probes in vitro.
Repair enzyme-containing extracts from a variety of cell types are used to analyse and compare DNA damage induced by oxygen radicals and excited molecules. The differing potentials of these extracts for recognising DNA damage leads to characteristic DNA damage profiles after treatment with superoxide (xanthine/xanthine oxidase), gamma-rays, chemically generated singlet oxygen, photosensitizers (rose bengal, methylene blue), UV254 and a 1,2-dioxetane. Three different types of damage profiles are distinguished and assigned to the predominant action of hydroxyl radicals, singlet oxygen or to the photoexcitation of thymine residues. The method applied in this study allows the analysis of DNA damage and the identification or exclusion of the participation of different ultimate reactive species without chemical identification of the lesions.
Epe
B
B
Institute of Pharmacology and Toxicology, University of Würzburg, F.R.G.
Mützel
P
P
Adam
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W
D001435:Bacteriophages; D004247:DNA / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D004279:DNA, Viral / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004720:Endonucleases / Q000378:metabolism; D005609:Free Radicals; D005720:Gamma Rays; D006571:Heterocyclic Compounds / Q000494:pharmacology; D008751:Methylene Blue / Q000494:pharmacology; D008837:Micrococcus / Q000201:enzymology; D010100:Oxygen / Q000494:pharmacology*; D012395:Rose Bengal / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000201:enzymology; D026082:Singlet Oxygen; D013481:Superoxides / Q000494:pharmacology; D014466:Ultraviolet Rays; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D004279:DNA, Viral; D005609:Free Radicals; D006571:Heterocyclic Compounds; D014970:Xanthines; D013481:Superoxides; D026082:Singlet Oxygen; D019820:Xanthine; D012395:Rose Bengal; D004247:DNA; D014969:Xanthine Oxidase; D004720:Endonucleases; D010100:Oxygen; D008751:Methylene Blue