Biochemical characterization of the Ca2+ release channel of skeletal and cardiac sarcoplasmic reticulum.
Rapid mixing-vesicle ion flux and planar lipid bilayer-single channel measurements have shown that a high-conductance, ligand-gated Ca2+ release channel is present in 'heavy', junctional-derived membrane fractions of skeletal and cardiac muscle sarcoplasmic reticulum. Using the release channel-specific probe, ryanodine, a 30S protein complex composed of polypeptides of Mr approximately 400,000 has been isolated from cardiac and skeletal muscle. Reconstitution of the complex into planar lipid bilayers has revealed a Ca2+ conductance with properties characteristic of the native Ca2+ release channel.
Meissner
G
G
Department of Biochemistry, University of North Carolina, Chapel Hill 27599.
Rousseau
E
E
Lai
F A
FA
Liu
Q Y
QY
Anderson
K A
KA
D000227:Adenine Nucleotides / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D015220:Calcium Channels / Q000032:analysis*; D002147:Calmodulin / Q000494:pharmacology; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000032:analysis*; D009206:Myocardium / Q000032:analysis*; D012433:Ryanodine; D012519:Sarcoplasmic Reticulum / Q000032:analysis*
D000227:Adenine Nucleotides; D015220:Calcium Channels; D002147:Calmodulin; D012433:Ryanodine; D002110:Caffeine; D008274:Magnesium; D002118:Calcium