Cyclic AMP--an intracellular second messenger for interleukin 1.
We demonstrated that interleukin 1 (IL-1), a potent peptide mediator in immune and inflammatory responses, stimulates the synthesis of cAMP in a variety of IL-1-responsive cell targets. We also showed that cAMP analogs and cAMP-inducing agents can replace IL-1 in the induction of interleukin 2 receptors on lymphocytes as well as in phytohemagglutinin-induced murine thymocyte proliferation. By use of IL-1 and the cAMP-inducer, forskolin, a direct correlation between the induced level of cAMP and the degree of lymphocyte interleukin 2 receptor expression or thymocyte proliferation was established. Our results indicate that cAMP may be an important intracellular second messenger for IL-1.
Shirakawa
F
F
Department of Microbiology and Immunology, Wake Forest University Medical Center, Winston-Salem, NC 27103.
Yamashita
U
U
Chedid
M
M
Mizel
S B
SB
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D005434:Flow Cytometry; D007375:Interleukin-1 / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D015290:Second Messenger Systems; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
D007375:Interleukin-1; D015375:Receptors, Interleukin-2; D005576:Colforsin; D000242:Cyclic AMP