title: Structural correlates of stimulated and inhibited secretion: electron microscopic observations of somatotrophs in perifused rat pituitary.
abstract: The in vitro release of stored intracellular rat growth hormone (rGH) in response to several secretagogues suggests the functional division of rGH storage into at least two 'compartments' or 'pools'. The first is an immediately releasable compartment whose response is brief. The second is a compartment which responds to more prolonged secretory demands. These observations are consistent with either a single, homogeneous population of somatotrophs, each of which exhibits functional compartmentalization of storage, or with heterogeneous populations of somatotrophs, each family of which provides one of the observed responses. We sought anatomical correlates of this functional compartmentalization using a perifusion/morphometric system which permitted examination of the first model while not excluding the second model. We selected for study an established perifusion protocol whose behavior was consistent and whose previous results suggested phases of both filling and emptying of the immediate release pool. Five parallel perifusions of pituitary fragments were run. The fifth perifusion was used to monitor rGH release and to confirm that the experiment had behaved in standard fashion. The first pituitary chamber was dismantled during basal perifusion to obtain tissue for microscopy, the next during exposure to 25 nM SRIF, the third during exposure to both 25 nM SRIF and 1 mM (Bu)2cAMP, and the fourth shortly after the rapid release which followed SRIF withdrawal. Somatotroph granulation was decreased by 54% in the presence of SRIF, and then increased by 45% with the addition of (Bu)2cAMP. The intracellular distribution of granules also fluctuated in relation to the stimulatory and inhibitory secretagogues. In addition, secondary lysosomes increased by 549% during SRIF-induced inhibition of rGH release.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.forename: M E
authors.initials: ME
authors.lastname: Stachura
authors.forename: A
authors.initials: A
authors.lastname: Costoff
authors.forename: J M
authors.initials: JM
authors.lastname: Tyler
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D013006:Growth Hormone / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008854:Microscopy, Electron; D010477:Perfusion; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013004:Somatostatin / Q000494:pharmacology
chemical_list: D013004:Somatostatin; D003994:Bucladesine; D013006:Growth Hormone