Cloning, nucleotide sequence and molecular evolution of a rabbit processed metallothionein MT-2 pseudogene.
A rabbit metallothionein-2 pseudogene (MT-2 psi) has been isolated from a partial rabbit genomic library. Its unusual sequence shows evidence of complex rearrangements involving recombination and deletion events. There are no intervening sequences, 3' poly A tract or 5' regulatory DNA sequences. The pseudogene is flanked by two sets of direct repeats (CT)3 GT(CT)4 and CTGG(G)CTC. They are most probably the sites of insertion of MT-2 psi in the rabbit genome. In addition, a number of repetitive DNA sequences are observed flanking the MT-2 psi gene. These are features of a processed retrogene.
Tam
Y C
YC
Department of Microbiology, Faculty of Medicine, Centre Hospitalier Universitaire, Sherbrooke, Quebec, Canada.
Hassan
M
M
Chopra
A
A
Thirion
J P
JP
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004251:DNA Transposable Elements; D008668:Metallothionein / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D011544:Pseudogenes; D011817:Rabbits; D011994:Recombinant Proteins / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D000596:Amino Acids; D004251:DNA Transposable Elements; D011994:Recombinant Proteins; D004247:DNA; D008668:Metallothionein