title: Enhancement of the apparent cleavage specificities of restriction endonucleases: applications to megabase mapping of chromosomes.
abstract: We have described how DNA methylases may be used to enhance the apparent specificities of restriction endonucleases [9-11, 17, 18] to generate DNA fragments averaging 6000 to 270,000,000 base pairs on random DNA. Taking into account the non-random arrangement of natural DNA, we have predicted the rarity of certain recognition sequences in the genomes of several species.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
authors.forename: M
authors.initials: M
authors.lastname: McClelland
authors.forename: M
authors.initials: M
authors.lastname: Nelson
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000378:metabolism; D015254:DNA Modification Methylases / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D004247:DNA; D015254:DNA Modification Methylases; D004262:DNA Restriction Enzymes