Chromosome-encoded beta-lactamases of Citrobacter diversus. Interaction with beta-iodopenicillanate and labelling of the active site.
Both forms of the chromosome-encoded beta-lactamase of Citrobacter diversus react with beta-iodopenicillanate at a rate characteristic of class A beta-lactamases. The active site of form I was labelled with the same reagent. The sequence of the peptide obtained after trypsin hydrolysis is identical with that of a peptide obtained in a similar manner from the chromosome-encoded beta-lactamase of Klebsiella pneumoniae.
Amicosante
G
G
Università degli Studi dell'Aquila Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Italy.
Oratore
A
A
Joris
B
B
Galleni
M
M
Frère
J M
JM
Van Beeumen
J
J
D000595:Amino Acid Sequence; D001665:Binding Sites; D002876:Chromosomes, Bacterial / Q000201:enzymology*; D002954:Citrobacter / Q000201:enzymology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D010397:Penicillanic Acid / Q000494:pharmacology / Q000031:analogs & derivatives; D010446:Peptide Fragments / Q000032:analysis; D065093:beta-Lactamase Inhibitors
D007527:Isoenzymes; D010446:Peptide Fragments; D065093:beta-Lactamase Inhibitors; D010397:Penicillanic Acid; C031528:6-iodopenicillanic acid