title: Inactivation of calcium channels in rat pituitary intermediate lobe cells.
abstract: The voltage-dependent inactivation of Ca currents was explored in dissociated intermediate lobe (IL) cells from the rat pituitary. On the basis of current-voltage relations two main inward currents could be identified in this cell, a transient current, (I-t), and a sustained current, (I-s). Inactivation was explored either by changing the holding potential and testing the change in the inward currents during a brief test pulse, or, by depolarizing the membrane and following the decay of the evoked inward current. Three current decay rates were identified, each with a characteristic dependence on membrane potential. The fastest decay rate (tau 1), was attributed to the inactivation of the I-t current and had a value of 57 ms at -40 mV, decreasing to 10 ms at -10 mV (extrapolated value of 6 ms at 0 mV). The other two decay rates, tau 2 and tau 3, decreased monotonically with depolarization of the membrane potential and reflected the inactivation of the I-s current with values of 1.8 and 20 s at 0 mV. I-s inactivation and reactivation was found to occur even in the normal resting potential range of this cell. These properties of the calcium channels can explain the voltage-dependent inactivation of secretion that has been observed previously in this and other secretory cells. In addition, they suggest that calcium currents, and hence secretion, may be modulated by external factors that cause small, but sustained, changes in the resting potential of the IL cell.
authors.affiliation: Laboratory of Biophysics, NINCDS, Bethesda, MD 20892.
authors.forename: E F
authors.initials: EF
authors.lastname: Stanley
authors.forename: J T
authors.initials: JT
authors.lastname: Russell
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000502:physiology; D015220:Calcium Channels / Q000502:physiology*; D002478:Cells, Cultured; D004558:Electric Stimulation; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D015220:Calcium Channels; D001464:Barium; D000255:Adenosine Triphosphate