title: Migration inhibition of an epithelial cell line by s-Con A and the effect on its invasiveness.
abstract: In an attempt to delineate the role of tumor-cell motility in the process of invasion, we compared the migration of NBT II in a two-dimensional migration assay with its migration in a three-dimensional invasion assay. Both systems were maintained with and without succinylated concanavalin A (s-Con A) dissolved in the culture medium. This lectin has a reversible inhibitory effect on the migration of cells in vitro. The migration of NBT-II aggregates, seeded in flasks containing 200 micrograms/ml s-Con A or without s-Con A, was studied by time-lapse photomicrography. In the presence of s-Con A, migration was immediately stopped. When the treated medium was replaced by culture medium to which 10 mM alpha-methyl-mannose was added, the inhibition of migration was abolished. The invasive capacity of NBT II in the presence or absence of s-Con A was studied by confronting precultured fragments of 9- to 11-day-old embryonic chick heart (0.4 mm in diameter) with NBT-II aggregates (0.2 mm) made in the presence or absence of s-Con A. Light microscopy showed no difference in the extent of invasion. To demonstrate the presence of s-Con A in the invading tumor cells, immunoperoxidase staining for Con A was done. The treated cultures stained positively while the controls were negative. The data presented here question the correlation between tumor-cell motility in two-dimensional system and the invasive behavior of these cells in three dimensions, and implies that the ability or inability of cells to migrate on plastic does not necessarily reflect their invasiveness in vitro.
authors.forename: W
authors.initials: W
authors.lastname: Schroyens
authors.forename: R
authors.initials: R
authors.lastname: Tchao
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000473:pathology*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003208:Concanavalin A / Q000031:analogs & derivatives* / Q000494:pharmacology; D004848:Epithelium / Q000473:pathology; D008722:Methods; D009361:Neoplasm Invasiveness; D051381:Rats; D011919:Rats, Inbred Strains; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
chemical_list: D003208:Concanavalin A; C019558:succinylconcanavalin A