Evidence that noradrenaline increases pyruvate dehydrogenase activity and decreases acetyl-CoA carboxylase activity in rat interscapular brown adipose tissue in vivo.
The rate of fatty acid synthesis in interscapular brown adipose tissue of female cold-adapted rats, as measured by the incorporation of 3H from 3H2O into tissue lipid, was decreased by about 70% after injection of noradrenaline. There was a similar decrease in the activity of acetyl-CoA carboxylase. In contrast, the proportion of pyruvate dehydrogenase in its active non-phosphorylated form was greatly increased after injection of noradrenaline. This finding suggests that the oxidation of glucose may be important in noradrenaline-induced thermogenesis in rat brown adipose tissue.
Gibbins
J M
JM
Denton
R M
RM
McCormack
J G
JG
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D005227:Fatty Acids / Q000096:biosynthesis; D005260:Female; D008025:Ligases / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature
D005227:Fatty Acids; D011768:Pyruvate Dehydrogenase Complex; D011433:Propranolol; D008025:Ligases; D000103:Acetyl-CoA Carboxylase; D009638:Norepinephrine