Cloning of nisin resistance determinant and replication origin on 7.6-kilobase EcoRI fragment of pNP40 from Streptococcus lactis subsp. diacetylactis DRC3.
The nisin resistance determinant and an origin of replication on pNP40, a plasmid of about 60 kilobases that is present in Streptococcus lactis subsp. diacetylactis DRC3, was cloned on a 7.6-kilobase EcoRI fragment. When self-ligated, this fragment existed as an independent replicon (pFM011) and contained a 2.6-kilobase EcoRI-XbaI fragment encoding nisin resistance.
Froseth
B R
BR
Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108.
Herman
R E
RE
McKay
L L
LL
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D005819:Genetic Markers; D013294:Lactococcus lactis / Q000187:drug effects / Q000235:genetics*; D009561:Nisin / Q000494:pharmacology*; D011815:R Factors; D012093:Replicon
D004269:DNA, Bacterial; D005819:Genetic Markers; D009561:Nisin; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI