Inhibitory effects of mannose and N-acetylneuraminic acid on the envelope and the adherence of a Neisseria gonorrhoeae isolate to rabbit mesentery.
Sugars and sugar derivatives were investigated as potential inhibitors of adherence of Neisseria gonorrhoeae; in this experiment rabbit mesentery was used as a reliable and convenient tissue. The adherence of N. gonorrhoeae isolate 2299 was reduced by half and more, as compared to controls, in the presence of 1,0 mg N-acetylneuraminic acid or D-mannose per ml of culture media, but not by the other sugars tested. Our data suggest that the N. gonorrhoeae isolate used in this work has mannose-binding related activity which participates in the process of its adherence to rabbit mesentery cells. In addition we observed that pretreatment of rabbit mesentery with concanavalin A, but not with peanut and wheat germ agglutinins, markedly decreased the adherence of gonococci as determined by microscopic counts of adhering bacterial cells.
Jacques
M
M
Turgeon
P L
PL
Mathieu
L G
LG
de Repentigny
J
J
D000268:Adhesiveness; D000818:Animals; D010861:Fimbriae, Bacterial / Q000502:physiology; D066298:In Vitro Techniques; D008358:Mannose / Q000494:pharmacology*; D008643:Mesentery / Q000382:microbiology; D019158:N-Acetylneuraminic Acid; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000502:physiology; D011817:Rabbits; D012794:Sialic Acids / Q000494:pharmacology*
D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid; D008358:Mannose