[Use of cultured, virus-infected cells to study the biogenesis of polarity of epithelial cells].
The major characteristic of the eucaryote cell is the presence of specialized organelles in which macromolecular components responsible for various subcellular functions are segregated. The membranes of these organelles serve not only as divisions between the various cytoplasmic compartments, but also provide scaffolding within which the macromolecular complexes of the organelle assemble and become functionally integrated. It is obvious that because of the degree of complexity resulting from the existence of numerous compartments and membrane systems, the development of a genetic programme in a eucaryote cell requires not only the transcription of specific genes and translation in the cytoplasma of the resultant messenger RNA, but also the activity of mechanisms which ensure that each polypeptide reaches the site of its function, which may be in the cytosol, in a membrane, or in the luminal cavity of an organelle. In the special case of membrane proteins, such mechanisms must result not only in the specific distribution of polypeptides newly synthesized in the various types of cell membrane, but also the arrangement of them required in the lipid bi-layer necessary for their normal function.
Sabatini
D D
DD
Department of Cell Biology, New York University School of Medicine, NY 10016.
Ivanov
I E
IE
Gottlieb
T A
TA
Compton
T
T
Gonzalez
A
A
Beaudry
G
G
Rindler
M J
MJ
D000818:Animals; D001692:Biological Transport; D002451:Cell Compartmentation; D002454:Cell Differentiation; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000502:physiology; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D006023:Glycoproteins / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology; D008854:Microscopy, Electron; D011956:Receptors, Cell Surface / Q000502:physiology; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
D006023:Glycoproteins; D011956:Receptors, Cell Surface; D014764:Viral Proteins
Utilisation de cultures de cellules infectées par des virus pour l'étude de la biogénèse de la polarité des cellules épithéliales.