Granulocyte-macrophage colony formation in vitro using human non-phagocytic bone marrow cells.
A technique employing silica particles was used to remove cells producing endogenous colony-stimulating factor (CSF) to allow measurement of the level of colony-forming units in culture (CFU-C) in human bone marrow cells. In comparison with the glass-adherence technique, this new approach resulted in a more complete degree of removal of CSF-producing cells and formation of more colonies. This method seems to be useful for performing an accurate assay of exogenous CSF.
Ohhara
N
N
First Dept. of Internal Medicine, Kyushu University, Fukuoka, Japan.
Okamura
S
S
Hayashi
S
S
Otsuka
T
T
Niho
Y
Y
D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000187:drug effects; D002469:Cell Separation; D003114:Colony-Forming Units Assay / Q000379:methods*; D003115:Colony-Stimulating Factors / Q000032:analysis; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D012822:Silicon Dioxide
D003115:Colony-Stimulating Factors; D012822:Silicon Dioxide