Fabrication of key components of a receptor-based biosensor.
A three-pronged approach was taken to the development of receptor-based bisensors. First, asymmetric bilayer membranes were developed with one monolayer adaptable to the particular receptor of interest and the other monolayer polymerized to enhance membrane stability. Second, alamethicin and calcium channel complexes were introduced into the stabilized membrane and tested for ion-channel function. Third, a porous support for the receptor-containing membrane was fabricated, which is compatible with silicon technology. Preliminary devices incorporating these components were constructed.
Ligler
F S
FS
Bio/Molecular Engineering Branch, Naval Research Laboratory, Washington, DC 20375-5000.
Fare
T L
TL
Seib
K D
KD
Smuda
J W
JW
Singh
A
A
Ahl
P
P
Ayers
M E
ME
Dalziel
A
A
Yager
P
P
D000408:Alamethicin / Q000032:analysis; D000818:Animals; D015374:Biosensing Techniques; D015220:Calcium Channels / Q000032:analysis; D002417:Cattle; D004553:Electric Conductivity; D004566:Electrodes; D004867:Equipment Design; D008024:Ligands; D008051:Lipid Bilayers / Q000032:analysis; D011956:Receptors, Cell Surface; D013499:Surface Properties
D015220:Calcium Channels; D008024:Ligands; D008051:Lipid Bilayers; D011956:Receptors, Cell Surface; D000408:Alamethicin