Virus removal or inactivation in hemoglobin solutions by ultrafiltration or detergent/solvent treatment.
Two procedures to eliminate virus infectivity from hemoglobin solutions at ambient temperature were evaluated. In the first, virus removal was assessed during the ultrafiltration of hemoglobin solutions through a membrane with a nominal molecular weight cut-off of 100,000 Daltons. The results of this study demonstrated that less than 0.1% of any virus originally spiked into the solution was detectable in the ultrafiltrate. In the second procedure the inactivation of viruses in hemoglobin solutions incubated with tri(n-butyl)phosphate mixed with sodium cholate was studied. Greater than 99% of each of the enveloped viruses tested was inactivated during the first 15 minutes of incubation with greater than 10(5) plaque forming units/ml of each being inactivated after one to six hours. No inactivation of the non-enveloped poliovirus was effected by this treatment. The data imply that both ultrafiltration and detergent/solvent incubation may reduce virus infectivity in hemoglobin solutions, but neither method yields a completely virus free product.
Bechtel
M K
MK
Travenol Laboratories, Inc., Hyland Therapeutics Research Facility, Duarte, California 91010.
Bagdasarian
A
A
Olson
W P
WP
Estep
T N
TN
D019826:Cholic Acid; D002793:Cholic Acids; D004340:Drug Contamination; D006454:Hemoglobins; D011558:Herpesvirus 1, Suid / Q000302:isolation & purification / Q000502:physiology; D008970:Molecular Weight; D010755:Organophosphates; D009943:Organophosphorus Compounds; D017955:Poliovirus / Q000302:isolation & purification / Q000502:physiology; D012845:Sindbis Virus / Q000302:isolation & purification / Q000502:physiology; D014462:Ultrafiltration; D014721:Vesicular stomatitis Indiana virus / Q000302:isolation & purification / Q000502:physiology; D018406:Virus Physiological Phenomena; D014780:Viruses / Q000302:isolation & purification*
D002793:Cholic Acids; D006454:Hemoglobins; D010755:Organophosphates; D009943:Organophosphorus Compounds; C016484:hemoglobin, stroma free; C009524:tributyl phosphate; D019826:Cholic Acid