[Transcription of the genome of Pseudomonas aeruginosa phage-transposon D3112 in an homologous host].
In vivo transcription of Pseudomonas aeruginosa transposable phage D3112 was studied. The 3H-labelled RNA isolated from the lysogenic cells PAO1 (D3112cts) after heat induction was hybridized with D3112 DNA to estimate phage-specific RNA. Two main stages of D3112 transcription, including transcription of the early (first 6-8 min) and the late (after 8th min) genes, were revealed. The transcription rate of D3112 early genes achieves the maximum at 3-5 min, being reduced to the minimum at 6-8 min after heat induction. These data point to the existence of negative regulation of D3112 early genes' transcription. The influence of ts mutations in early A, B and C genes and in late ts25 gene on transcription of D3112 phage was studied. It is shown that the genes A and B have no effect on the late transcription, while gene C regulates transcription of the D3112 late genes.
Bidnenko
E M
EM
Akhverdian
V Z
VZ
Ianenko
A S
AS
Krylov
V N
VN
D001435:Bacteriophages / Q000235:genetics*; D004251:DNA Transposable Elements; D005814:Genes, Viral; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
D004251:DNA Transposable Elements; D014764:Viral Proteins
Izuchenie transkriptsii genoma faga-transpozona D3112 Pseudomonas aeruginosa v gomologichnom khoziaine.