Step-wise progression of mammalian 10-kb DNA replication intermediates to mature chromatin.
In mammalian DNA synthesis the primary replication intermediates are joined to larger intermediates. After the joining process is complete one can detect a distinct stage called the post-elongation stage. Furthermore a 10-kb DNA1 population is detected before the post-elongation stage whereas a 10-kb DNA2 population is part of this stage DNA. When cells are treated with 3-aminobenzamide, an inhibitor of poly(ADP-ribose) synthetase, an altered post-elongation-stage DNA was detected, which does not contain 10-kb fragments. The step(s) affected by 3-aminobenzamide prevents the appearance of 10-kb DNA in the post-elongation stage. The drug effect is reversible with the appearance of 10-kb DNA in the post-elongation stage when the cells are washed free of drug. Hence there is a step-wise progression from 10-kb DNA, via the post-elongation stage, to mature chromatin.
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Karolinska Hospital, Stockholm, Sweden.
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D001549:Benzamides / Q000494:pharmacology; D002460:Cell Line; D002843:Chromatin / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D004720:Endonucleases; D006801:Humans; D008545:Melanoma / Q000378:metabolism; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013936:Thymidine / Q000378:metabolism
D001549:Benzamides; D002843:Chromatin; D004273:DNA, Neoplasm; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013936:Thymidine