Comparison of the effects of one-side- and two-side Mg2+ on the reconstitution of mitochondrial H+-ATPase.
Three types of proteoliposome containing mitochondrial H+-ATPase have been prepared: Mg2+-'free', one-side and two-side Mg2+-containing proteoliposomes. The ATPase activity as well as its sensitivity to oligomycin or N,N'-dicyclohexylcarbodiimide of the three proteoliposome preparations has been compared. They decreased in the order: L X (H+-ATPase)+ Mg2+ greater than L X (H+-ATPase)-+ Mg2+ greater than L X (H+-ATPase)-Mg2+. The fluidity of the proteoliposomes has also been compared by fluorescence polarization probes diphenylhexatriene (DPH) or 7-(9-anthroyloxy)stearic acid (7-AS). The degree of polarization for DPH in these proteoliposomes decreased in the order: L X (H+-ATPase) +Mg2+ greater than L X (H+-ATPase)-+ Mg2+ greater than L X (H+-ATPase)-Mg2+, while that for 7-AS: L X (H+-ATPase) +Mg2+ approximately equal to L X (H+-ATPase)-+ Mg2+ greater than L X (H+-ATPase) -Mg2+.
Yang
F Y
FY
Zhang
X F
XF
D000818:Animals; D005454:Fluorescence Polarization; D008274:Magnesium / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000201:enzymology*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D013552:Swine
D006180:Proton-Translocating ATPases; D008274:Magnesium