title: The inhibition of mitochondrial F1-ATPase by 1,5-difluoro-2,4-dinitrobenzene.
abstract: 1,5-Difluoro-2,4-dinitrobenzene completely inhibits F1-ATPase when it is used at micromolar concentrations and the inhibitor/enzyme molar ratio is equal to 3. The inhibition can be reversed by dithiothreitol treatment. 7-Chloro-4-nitrobenzofurazan treatment of F1-ATPase does not prevent the reaction of the enzyme with 1,5-difluoro-2,4-dinitrobenzene. The 1,5-difluoro-2,4-dinitrobenzene-induced inhibition is thought to be a result of the modification of a tyrosine residue with pK 9.1.
authors.forename: A A
authors.initials: AA
authors.lastname: Akhrem
authors.forename: M A
authors.initials: MA
authors.lastname: Kisel
authors.forename: I A
authors.initials: IA
authors.lastname: Kozlov
authors.forename: I S
authors.initials: IS
authors.lastname: Tsybovsky
authors.forename: E N
authors.initials: EN
authors.lastname: Vulfson
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology*; D009578:Nitrobenzenes / Q000494:pharmacology*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*
chemical_list: D009578:Nitrobenzenes; D000255:Adenosine Triphosphate; D004139:Dinitrofluorobenzene; D006180:Proton-Translocating ATPases; D009327:4-Chloro-7-nitrobenzofurazan; C002041:1,5-difluoro-2,4-dinitrobenzene; D004229:Dithiothreitol