title: Effect of ischemia on calcium-dependent fluorescence transients in rabbit hearts containing indo 1. Correlation with monophasic action potentials and contraction.
abstract: The effects of acute global ischemia on cytosolic calcium transients were studied in perfused rabbit hearts loaded with the fluorescent calcium indicator indo 1. Indo 1-loaded hearts were illuminated at 360 nm, and fluorescence was recorded simultaneously at 400 and 550 nm from the epicardial surface of the left ventricle. The F400/F550 ratio was calculated by an analog circuit, which allowed cancellation of optical motion artifact. Resulting calcium transients demonstrated a rapid upstroke and slow decay similar to those recorded in isolated ventricular myocytes. Global ischemia rapidly suppressed contraction, but it produced a concurrent increase in the systolic and diastolic levels of the calcium transients, together with an increase in the duration of the peak. The effects of ischemia were reversed by reperfusion, inhibited by verapamil, and mimicked by perfusion of nonischemic hearts with acidified (CO2-rich) solution. In addition to elevation of the calcium transients, ischemia caused a pattern of intracellular calcium alternans that was discernible after 2-3 minutes. The pattern of alternans was stable at a given epicardial site, but it could be out of phase at different sites. Similar nonuniformities were observed in contraction strength and in the duration of monophasic action potentials recorded immediately adjacent to the fiber-optic probe. Abnormalities in intracellular calcium may be a causal factor in the loss of electrical and mechanical synchrony in the acutely ischemic heart.
authors.affiliation: Division of Cardiology, Stanford University School of Medicine, California 94305.
authors.forename: H C
authors.initials: HC
authors.lastname: Lee
authors.forename: R
authors.initials: R
authors.lastname: Mohabir
authors.forename: N
authors.initials: N
authors.lastname: Smith
authors.forename: M R
authors.initials: MR
authors.lastname: Franz
authors.forename: W T
authors.initials: WT
authors.lastname: Clusin
mesh_terms: D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D003327:Coronary Disease / Q000378:metabolism* / Q000503:physiopathology; D005453:Fluorescence; D005456:Fluorescent Dyes; D007211:Indoles; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism; D010477:Perfusion; D011817:Rabbits; D014700:Verapamil / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D005456:Fluorescent Dyes; D007211:Indoles; D014700:Verapamil; C048960:indo-1; D002118:Calcium