title: [Culture methods of human liver cells].
abstract: Attempts to isolate and cultivate human liver cells have been described. Many viable liver cells have been obtained when dissociated with collagenase followed by dispase. The morphology and differentiated functions have been maintained for more than 3 weeks when human fetal liver cells were cultured not only in medium containing 10(-3) M hydrocortisone, but also on collagen gel substrates with 5 x 10(-7) M hydrocortisone. The colony-forming capacity of primary cultured fetal human livers has also been described in the presence of conditioned medium.
authors.affiliation: Division of Pathology, Okayama University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tokiwa
authors.forename: Y
authors.initials: Y
authors.lastname: Kusaka
authors.forename: A
authors.initials: A
authors.lastname: Muraoka
authors.forename: J
authors.initials: J
authors.lastname: Sato
mesh_terms: D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D003584:Cytological Techniques; D010450:Endopeptidases; D005333:Fetus; D005782:Gels; D006801:Humans; D006854:Hydrocortisone; D008099:Liver / Q000166:cytology*; D003012:Microbial Collagenase
chemical_list: D003470:Culture Media; D005782:Gels; D003094:Collagen; D010450:Endopeptidases; D003012:Microbial Collagenase; C023139:dispase; D006854:Hydrocortisone