The 19 S multicatalytic "prosome" proteinase is a constitutive enzyme in HeLa cells.
HeLa human carcinoma cells contain a high molecular mass (Mr approximately 700,000) proteinase that is identical with the ubiquitous eukaryotic 19 S cylindrical "prosome" particle. Prosomes constitute 0.6% of cell bulk protein as determined by immunochemical analysis. The prosome proteinase is not a heat shock protein, nor is it induced by cell crowding in culture or by serum deprivation. Pulse-chase experiments showed that the proteinase is relatively stable in the cells (t 1/2 approximately 5 days).
Hendil
K B
KB
August Krogh Institute, Universitetsparken, Copenhagen, Denmark.
D002401:Cathepsin B / Q000378:metabolism; D002455:Cell Division; D010450:Endopeptidases / Q000378:metabolism*; D006207:Half-Life; D006367:HeLa Cells / Q000201:enzymology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000201:enzymology
D006360:Heat-Shock Proteins; D010450:Endopeptidases; D002401:Cathepsin B