Identification of vasopressin-like peptides in the plasma of a patient with the syndrome of inappropriate secretion of antidiuretic hormone and an oat cell carcinoma.
In this study we have identified and characterized several vasopressin-like peptides in the plasma of a patient with the syndrome of inappropriate antidiuretic hormone secretion and oat cell carcinoma of the lung. Immunoreactive plasma vasopressin was measured after gel filtration (Sephadex G-25) or C-18 cartridge extraction using two different region-specific antisera: AS1 and AS2. Antiserum AS1 is more specifically directed towards the antigenic site of the hexapeptidic ring of arginine-vasopressin (AVP), whereas AS2 is more specifically directed towards the C-terminal region of AVP. Unexpectedly, the Sephadex G-25 gel filtration elution profile of the immunoreactive vasopressin was very heterogeneous, indicating the presence of several molecular species. After extraction of total AVP and AVP-like peptides of this plasma, an unusual AS1/AS2 ratio of immunoreactivity was observed, suggesting the presence of vasopressin-like peptides which differ from AVP in the C-terminus.
Smitz
S
S
Radioimmunoassay Laboratory, University of Liège, CHU, Belgium.
Legros
J J
JJ
Franchimont
P
P
le Maire
M
M
D000368:Aged; D001127:Arginine Vasopressin / Q000097:blood; D018288:Carcinoma, Small Cell / Q000097:blood*; D002850:Chromatography, Gel; D006801:Humans; D007177:Inappropriate ADH Syndrome / Q000097:blood*; D008175:Lung Neoplasms / Q000097:blood*; D008968:Molecular Conformation; D011863:Radioimmunoassay; D014357:Trypsin / Q000494:pharmacology; D014667:Vasopressins / Q000097:blood*
D014667:Vasopressins; D001127:Arginine Vasopressin; D014357:Trypsin