The role of calpain and protein kinase C in activation of human neutrophils.
These and earlier findings suggest that certain neutrophil biochemical responses induced by external stimuli are mediated by the reorganization of the cytoskeletal-membrane interactions. This rearrangement of the intracellular architecture appears to result from a coordinated and integrated operation of two enzymes, namely PKC and calpain. This conclusion is schematically represented in a comprehensive model, discussed in the introduction and derived from a number of experimental observations. In focusing the importance of cytoskeletal reorganization, signal-directed proteolysis, induced by specific PKC-mediated phosphorylation, appears to play a fundamental role in cell functions such as positioning and fusion of organelles with plasma membranes and possibly locomotion and transport.
Pontremoli
S
S
Institute of Biochemistry, University of Genoa, Italy.
Melloni
E
E
D002154:Calpain / Q000097:blood* / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000097:blood; D006801:Humans; D007700:Kinetics; D009504:Neutrophils / Q000201:enzymology / Q000502:physiology*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000097:blood* / Q000502:physiology; D015398:Signal Transduction
D003598:Cytoskeletal Proteins; D011493:Protein Kinase C; D002154:Calpain