The effects of scleroderma serum on human microvascular endothelial cells. Induction of antibody-dependent cellular cytotoxicity.
To investigate the vascular immunopathology of systemic sclerosis, we developed a model consisting of human microvascular endothelial cells, leukocytes, and serum. Sera from 19% of the patients studied mediated antibody-dependent cellular cytotoxicity against endothelial cells. Some sera also mediated cytotoxicity against aortic endothelium and fibroblasts. K lymphocytes, the cells that mediate antibody-dependent cellular cytotoxicity, were identified in the skin of some patients. The sera alone were not cytotoxic or growth inhibitory, and did not affect endothelial prostacyclin production.
Marks
R M
RM
Centre for Immunology, University of New South Wales, Sydney, Australia.
Czerniecki
M
M
Andrews
B S
BS
Penny
R
R
D000920:Antibody-Dependent Cell Cytotoxicity; D002455:Cell Division / Q000187:drug effects; D002860:Chromium Radioisotopes; D000242:Cyclic AMP / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology*; D011464:Epoprostenol / Q000096:biosynthesis; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000145:classification; D010641:Phenotype; D011863:Radioimmunoassay; D012595:Scleroderma, Systemic / Q000276:immunology*; D012867:Skin / Q000166:cytology
D002860:Chromium Radioisotopes; D007074:Immunoglobulin G; D011464:Epoprostenol; D000242:Cyclic AMP