General characterization of human cytomegalovirus-specific proliferative CD4+ T cell clones.
Over 80 human T helper cell (Th) clones reactive with human cytomegalovirus (HCMV) were generated using purified whole Towne strain HCMV as the in vitro antigen. These cloned T cells are CD3+, CD4+, CD8- and proliferate specifically to HCMV. All of the clones tested produce interleukin 2 and gamma-interferon and failed to show HCMV-specific cytotoxicity or natural killer (NK) activity. Most of the Th clones recognize multiple laboratory-adapted and wild-type strains of HCMV. The Th clones were also tested for their reactivity to a major envelope glycoprotein complex (gcI) and a 64,000 dalton internal matrix protein. Our results show that both proteins as well as other unidentified protein(s) are involved in Th responses to HCMV.
Liu
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Sutton Immunobiology Research Laboratory, Children's Hospital, St. Paul, Minnesota 55102.
Eckhardt
J
J
Kari
B
B
Gehrz
R C
RC
D000956:Antigens, Viral / Q000302:isolation & purification; D002455:Cell Division; D002999:Clone Cells; D003587:Cytomegalovirus / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis; D010641:Phenotype; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000956:Antigens, Viral; D006023:Glycoproteins; D007376:Interleukin-2; D007371:Interferon-gamma