In-vitro study of the enkephalinergic hypothesis for non-adrenergic, non-cholinergic innervation in the cat stomach.
It has been suggested that enkephalins are involved in the gastric relaxation induced by stimulation of the non-adrenergic, non-cholinergic vagal fibres in the cat stomach. Experiments were therefore performed on strips of cat stomach. With longitudinal and circular gastric fundus and corpus strips from reserpinized cats, non-adrenergic, non-cholinergic relaxatory responses could be elicited by transmural electrical stimulation in Tyrode solution containing atropine and 5-hydroxytryptamine. Morphine, leu-enkephalin and met-enkephalin did not influence the tone of the strips or the relaxation evoked by stimulation at 8 Hz, and neither did the opioid antagonist, naloxone. These results do not support the enkephalinergic hypothesis for the non-adrenergic, non-cholinergic vagal fibres in the cat stomach.
Lefebvre
R A
RA
Bogaert
M G
MG
De Schaepdryver
A F
AF
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002415:Cats; D004743:Enkephalin, Leucine / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000494:pharmacology; D004745:Enkephalins / Q000502:physiology*; D005260:Female; D005769:Gastrointestinal Motility / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009020:Morphine / Q000494:pharmacology; D009270:Naloxone / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology; D013270:Stomach / Q000294:innervation*; D009435:Synaptic Transmission
D004745:Enkephalins; D018377:Neurotransmitter Agents; D012701:Serotonin; D009270:Naloxone; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D009020:Morphine; D001285:Atropine