A pharmacogenetic analysis of the effects of tetrahydrocannabinol (delta 9THC) on brain gamma glutamyl transpeptidase.
The effects of oral administration of (-)delta 9 Trans-Tetrahydrocannabinol (THC) on the membrane bound enzyme, gamma-glutamyl transpeptidase (gamma-GTP) was studied. Adult male mice were given an oral dose of THC on each of 5 consecutive days in a vehicle of sesame oil, coconut oil and tween. The dosage ranged from 2.5 to 10 mg/kg. The animals were decapitated and the brains dissected into eight regions. Each brain region was homogenized in Tris buffered saline and assayed for protein and gamma-GTP activity. The higher doses of THC produced higher levels of gamma-GTP activity in the brain regions studied.
Reyes
E
E
Jones
B C
BC
D000818:Animals; D001921:Brain / Q000187:drug effects*; D003342:Corpus Striatum / Q000201:enzymology; D013759:Dronabinol / Q000494:pharmacology*; D005838:Genotype; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D013759:Dronabinol; D005723:gamma-Glutamyltransferase