A gene fusion system using the aminoglycoside 3'-phosphotransferase gene of the kanamycin-resistance transposon Tn903: use in the yeast Kluyveromyces lactis and Saccharomyces cerevisiae.
The aminoglycoside 3'-phosphotransferase type I (APHI)-coding gene of the bacterial transposon Tn903 confers resistance to kanamycin on bacteria and resistance to geneticin (G418) on many eukaryotes. We developed an APHI fusion system that can be used in the study of gene expression in these organisms, particularly in yeasts. The first 19 codons of the KmR (APHI) gene can be deleted, and replaced by other genes in a continuous reading frame, without loss of APH activity. Examples of vector constructions are given which are adapted to the yeast Kluyveromyces lactis transformation system. Their derivatives containing the 2 mu origin of replication can also be used in Saccharomyces cerevisiae.
Chen
X J
XJ
Institut Curie, Centre Universitaire, Orsay, France.
Fukuhara
H
H
D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005796:Genes; D005800:Genes, Fungal; D019868:Kanamycin Kinase; D007613:Kanamycin Resistance / Q000235:genetics*; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D004718:Saccharomycetales / Q000235:genetics*
D004251:DNA Transposable Elements; D010770:Phosphotransferases; D019868:Kanamycin Kinase