A spectrophotometric method for calmodulin assay.
The activity of calmodulin as an activator of cAMP phosphodiesterase was assayed. AMP was hydrolyzed by 5'-nucleotidase, and the adenosine formed was measured by both liquid scintillation counting and spectrophotometry at 265 nm. Calmodulin activities measured by the two methods were equivalent, indicating that spectrophotometric assay of calmodulin can be used in place of the isotopic method.
Garg
U C
UC
Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Rai
N
N
Singh
Y
Y
Dhaunsi
G S
GS
Sidhu
G S
GS
Ganguly
N K
NK
Bhatnagar
R
R
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000502:physiology; D002147:Calmodulin / Q000032:analysis*; D000242:Cyclic AMP / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D002147:Calmodulin; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases