Human cytomegalovirus ICP22, the product of the HWLF1 reading frame, is an early nuclear protein that is released from cells.
We employed a murine monoclonal antibody (CH41) and a lambda gt11 library of human cytomegalovirus (CMV) DNA fragments to map the gene for a viral protein, denoted infected cell protein (ICP) 22, to the HWLF1 open reading frame in the S component of the CMV genome (0.92 to 0.93 map units). By using antibody CH41 in immunofluorescence, immunoprecipitation and immunoblotting analyses, ICP22 was readily detected as a beta (delayed early) gene product during viral growth. The cellular localization of this protein was found to be nuclear by immunofluorescence analysis; however, it partitioned with the cytoplasm when cells were fractionated with non-ionic detergents. Analysis of cell-free medium showed that a proportion of ICP22 was released from cells as a soluble protein at both early (24 h) and late (72 to 120h) times in infection. The function of this protein which has such diverse characteristics remains unknown.
Mocarski
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ES
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305.
Pereira
L
L
McCormick
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AL
D000911:Antibodies, Monoclonal; D003587:Cytomegalovirus / Q000235:genetics*; D015342:DNA Probes; D005814:Genes, Viral; D006801:Humans; D017874:Immediate-Early Proteins; D066298:In Vitro Techniques; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins
D000911:Antibodies, Monoclonal; D015342:DNA Probes; C057294:ICP22 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C078424:EUS1 protein, Equine herpesvirus 1