Immune production of interferon by cultured peripheral blood mononuclear cells from calves infected with BHV1 and PI3 viruses.
Peripheral blood mononuclear cells (PBMC) from calves infected with bovine herpesvirus type 1 (BHV1) or parainfluenza 3 virus (PI3) were cultured in vitro in the presence of inactivated specific antigen presented on MDBK cells. In the presence of inactivated antigen, PBMC from both BHV1-infected and control calves produced interferon (IFN)-alpha in 24 hour cultures. Altering the culture conditions did not result in the detection of immune-specific IFN produced by mononuclear cells from BHV1-infected calves. However, spontaneous IFN was detected in the absence of antigen in 24 hour cultures from infected animals: this IFN was pH 2 labile and completely neutralised by antiserum to recombinant bovine IFN-gamma. Spontaneous IFN-gamma production was only seen in calves following a second BHV1 inoculation, given four to seven weeks after the primary dose. In contrast PBMC cultures from PI3 virus-infected calves did not produce IFN-gamma spontaneously, but did so in cultures which contained inactivated PI3 antigen. Mononuclear cells from control animals failed to produce either IFN-alpha or -gamma when cultured with inactivated PI3 virus. IFN-gamma was detected in PBMC cultures after the primary infection, with no increase in production occurring following subsequent PI3 virus inoculations. Immunospecific production of IFN-gamma provides a simple method for monitoring cell-mediated immunity in BHV1- and PI3 virus-infected calves and can be used for evaluating the efficacy of vaccines against these viruses.
Townsend
J
J
Department of Clinical Veterinary Medicine, University of Cambridge.
Duffus
W P
WP
Williams
D L
DL
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D007111:Immunity, Cellular; D007241:Infectious Bovine Rhinotracheitis / Q000097:blood / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D010224:Parainfluenza Virus 3, Human; D018184:Paramyxoviridae Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary*
D007371:Interferon-gamma