Increased detection rate for varicella-zoster virus with combination of two techniques.
Standard cell culture and centrifugation culture were compared for the isolation of varicella-zoster virus from 337 clinical specimens. Of a total of 85 (25%) positive specimens, 67 (78.8%) were positive by centrifugation culture alone and 18 (21.2%) were positive by both methods. When epithelial cells from the specimen were included in the vial inoculum, these cells stained positive and increased the varicella-zoster virus detection rate by 30%.
West
P G
PG
SmithKline Bio-science Laboratories, Norristown, Pennsylvania 19403.
Aldrich
B
B
Hartwig
R
R
Haller
G J
GJ
D002460:Cell Line; D004847:Epithelial Cells; D014645:Herpesvirus 3, Human / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D012867:Skin / Q000382:microbiology*; D014776:Virus Cultivation / Q000379:methods