title: Human bronchial epithelial cells with integrated SV40 virus T antigen genes retain the ability to undergo squamous differentiation.
abstract: Human bronchial epithelial cells transformed by either DNA virus infection (SV40 or Adenovirus 12-SV40 hybrid virus) or transfection with the SV40 large T antigen gene were studied for their ability to undergo squamous differentiation when exposed to 12-O-tetradecanoylphorbol-13 acetate (TPA), transforming growth factor-beta 1 (TGF-beta 1), or fetal bovine serum (FBS), agents that induce the squamous differentiation of normal human bronchial epithelial cells. Squamous differentiation occurred in all ten T-antigen-positive cell cultures when they were exposed to either FBS or TGF-beta 1, but none differentiated when exposed to TPA. From one cell line, designated BEAS-2B, two subclones were isolated, one of which was induced to undergo squamous differentiation by FBS, and a second that failed to undergo squamous differentiation and was mitogenically stimulated when exposed to serum. These phenotypically different subclones provide a new in vitro cellular system for delineating the mechanism(s) of human bronchial epithelial cell squamous differentiation in response to FBS or TGF-beta 1.
authors.affiliation: Division of Cancer Etiology, National Cancer Institute, Bethesda, MD 20892.
authors.forename: Y
authors.initials: Y
authors.lastname: Ke
authors.forename: R R
authors.initials: RR
authors.lastname: Reddel
authors.forename: B I
authors.initials: BI
authors.lastname: Gerwin
authors.forename: M
authors.initials: M
authors.lastname: Miyashita
authors.forename: M
authors.initials: M
authors.lastname: McMenamin
authors.forename: J F
authors.initials: JF
authors.lastname: Lechner
authors.forename: C C
authors.initials: CC
authors.lastname: Harris
mesh_terms: D000956:Antigens, Viral / Q000378:metabolism*; D001980:Bronchi / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006801:Humans; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D012710:Serum Albumin, Bovine; C032975:12-O-tetradeca-2,4,6,8-tetranoylphorbol-13-acetate; D015291:Transforming Growth Factors; D013755:Tetradecanoylphorbol Acetate