Ca2+ analysis in arteries of spontaneously hypertensive rats by proton-induced X-ray emission.
Numerous investigations have shown that Ca2+ plays an important role in the pathogenesis of hypertension. Intracellular Ca2+ measurements have been carried out with Ca(2+)-selective electrodes and the Quin 2 method. The present study used particle induced X-ray emission to investigate the Ca2+ distribution in arterial smooth muscle. This method correlates Ca2+ distribution with the morphological structure of the tissue. The Ca2+ content of the aortas and renal arteries was measured in 22 spontaneously hypertensive and 11 normotensive rats. The findings demonstrate significant Ca(2+)-elevation in the aortas of spontaneously hypertensive rats compared with the aortas of normotensive rats (3124.9 +/- 790.0 versus 2031 +/- 478 micrograms Ca2+/g; P < 0.05), whereas there was only a tendency to Ca2+ elevation in the renal arteries of spontaneously hypertensive rats compared with normotensive rats (3336.0 +/- 1798.0 versus 1533.2 +/- 1082.0 micrograms Ca2+/g; NS). The Ca2+ was mainly detected in the muscle containing tunica media.
Spieker
C
C
Medizinische Universitäts Poliklinik, Münster, Federal Republic of Germany.
Heck
D
D
Zidek
W
W
Vetter
H
H
D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000378:metabolism; D001158:Arteries / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY; D012077:Renal Artery / Q000737:chemistry / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission
D002118:Calcium