title: Application of avidin-ferritin and peroxidase as contrasting electron-dense markers for simultaneous electron microscopic immunocytochemical labelling of glutamic acid decarboxylase and tyrosine hydroxylase in the rat arcuate nucleus.
abstract: A pre-embedding immunostaining procedure was developed using ferritin and peroxidase to enable simultaneous electron microscopic localization of two antigens in the same tissue section. This method was used to study the anatomic relationship between glutamic acid decarboxylase (GAD) immunoreactive axons and tyrosine hydroxylase (TH) - containing neurons of the rat arcuate nucleus. The findings provide ultrastructural evidence that GAD-immunoreactive terminals establish symmetric (Gray II) synapses on TH-reactive neurons.
authors.forename: C
authors.initials: C
authors.lastname: Leranth
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
authors.forename: N J
authors.initials: NJ
authors.lastname: MacLusky
authors.forename: M
authors.initials: M
authors.lastname: Shanabrough
authors.forename: F
authors.initials: F
authors.lastname: Naftolin
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000201:enzymology* / Q000648:ultrastructure; D001360:Avidin; D005260:Female; D005293:Ferritins; D005968:Glutamate Decarboxylase / Q000032:analysis*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D010544:Peroxidases; D051381:Rats; D011919:Rats, Inbred Strains; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D001360:Avidin; D005680:gamma-Aminobutyric Acid; D005293:Ferritins; D010544:Peroxidases; D014446:Tyrosine 3-Monooxygenase; D005968:Glutamate Decarboxylase