Characterization of a novel anti-peptide antibody that recognizes a specific conformation of the platelet-derived growth factor receptor.
Two site-specific anti-peptide antibodies (AbP1 and AbP2) were raised against the platelet-derived growth factor (PDGF) receptor. These two sites correspond to amino acid residues 977 through 988 (peptide 1) and 932 through 947 (peptide 2) of the murine PDGF receptor. Both antibodies recognized human and murine PDGF receptors in immunoprecipitation and immunoblotting analyses. None of the antibodies was directed to phosphotyrosine. One of the antibodies (AbP2) showed unusual antigen recognition specificity. This antibody specifically recognized the tyrosine-phosphorylated PDGF receptor and not the unphosphorylated native receptor, suggesting that recognition by this antibody requires a specific conformation that is induced by PDGF-stimulated autophosphorylation.
Bishayee
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Children's Hospital of Philadelphia, Pennsylvania.
Majumdar
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S
Scher
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CD
Khan
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D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology; D012516:Osteosarcoma; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011487:Protein Conformation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor
D000906:Antibodies; D000936:Antigen-Antibody Complex; D009842:Oligopeptides; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D011505:Protein-Tyrosine Kinases; D017479:Receptors, Platelet-Derived Growth Factor