herpes simplex virus infection accelerates the age-related reactivity of mouse trigeminal ganglia neurons with anti-mouse IgG antibody in immunostaining.
The detection of viral proteins is a major goal of research on herpes simplex virus type 1 (HSV-1) latency. We used immunostaining to detect viral proteins in neuronal cells of trigeminal ganglia of Balb/c mice after corneal inoculation with HSV-1 virus. Viral proteins were detected in the neurons during the acute stage of infection, i.e. within one week after inoculation. However, the detection of viral antigens at the latent stage of HSV-1 infection has proven difficult. We have detected age-dependent non-specific reactivity with anti-mouse IgG antibody in the neurons of 10-week-old or older uninfected mice. This reactivity is accelerated in HSV-1 infected mice, being seen at 6 weeks of age (2 weeks post infection). The accelerated reaction and impact of this effect is discussed in relation to detection of viral proteins during latency.
Wroblewska
Z
Z
Wistar Institute, Philadelphia, PA 19104.
Savage
K
K
Spivack
J G
JG
Fraser
N W
NW
D000375:Aging; D000818:Animals; D006561:Herpes Simplex / Q000382:microbiology* / Q000473:pathology; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000382:microbiology* / Q000473:pathology; D018139:Simplexvirus / Q000302:isolation & purification*; D014278:Trigeminal Nuclei / Q000254:growth & development / Q000382:microbiology* / Q000473:pathology; D014764:Viral Proteins / Q000032:analysis*
D007074:Immunoglobulin G; D014764:Viral Proteins