Regulation of yeast fructose-1,6-bisphosphatase in strains containing multicopy plasmids coding for this enzyme.
The structural gene for yeast fructose-1,6-bisphosphatase (FbPase) has been cloned by complementation of a strain carrying the fbp1-1 mutation and lacking FbPase activity. By Northern analysis it could be shown that the fbp1-1 mutation does not interfere with the transcription of the gene although no normal active enzyme is produced. Strains transformed with the cloned gene on a multicopy plasmid overproduce FbPase but are still subject to strong catabolite repression. Catabolite inactivation, however, is slowed down in such strains.
de la Guerra
R
R
Instituto de Investigaciones Biomédicas del CSIC, Facultad de Medicina, UAM, Madrid, Spain.
Valdés-Hevia
M D
MD
Gancedo
J M
JM
D015152:Blotting, Northern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005800:Genes, Fungal; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger; D006597:Fructose-Bisphosphatase