Comparison of extraction procedures for radioimmunoassay of human plasma somatostatin.
Recovery and chromatography profiles of human plasma somatostatin extracted by three different procedures were compared. Extraction of plasma using acid-acetone-ether and octadecylsilyl silica gave comparable recovery (84 +/- 1 and 93 +/- 2%, respectively), which were nearly twofold greater than recovery achieved with the acid-ethanol method (50 +/- 1%, p less than 0.01). Acid-acetone-ether and octadecylsilyl silica extraction eliminated nearly completely immunoreactive material other than somatostatin-14 and -28, whereas the acid-ethanol-ether method did not. Because of its simplicity, octadecylsilyl silica is probably the method of choice for extraction of somatostatin from human plasma.
Gottesman
I S
IS
Gerich
J E
JE
D000096:Acetone; D004986:Ether; D006801:Humans; D007457:Iodine Radioisotopes; D008722:Methods; D011863:Radioimmunoassay; D012822:Silicon Dioxide; D013004:Somatostatin / Q000097:blood*
D007457:Iodine Radioisotopes; C026679:octadecylsilica; D004986:Ether; D000096:Acetone; D013004:Somatostatin; D012822:Silicon Dioxide