Immunoprecipitation of the in vitro translation product of rabbit muscle protein phosphatase C-I mRNA.
Rabbit muscle polyA+ mRNA was translated in vitro using a rabbit reticulocyte lysate system in the presence of [35S]methionine. A mouse monoclonal antibody to the catalytic subunit of rabbit muscle phosphorylase phosphatase ("phosphatase C-I") was used to immunoprecipitate the products which were then analyzed by SDS-PAGE and autoradiography. These studies showed that the major product of the phosphatase mRNA is a single ca. 36 kDa polypeptide. These findings are significant in view of suggestions that the catalytic subunit is derived from a larger precursor, and in view of the molecular cloning of two cDNAs for the phosphatase, which encode polypeptides of 35.4 kDa and 37.5 kDa, respectively.
Zheng
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Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101.
Lee
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D000818:Animals; D000911:Antibodies, Monoclonal; D066298:In Vitro Techniques; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics*; D009132:Muscles / Q000201:enzymology; D010455:Peptides / Q000032:analysis; D010749:Phosphoprotein Phosphatases / Q000032:analysis / Q000235:genetics*; D011061:Poly A / Q000032:analysis; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D009124:Muscle Proteins; D010455:Peptides; D012333:RNA, Messenger; D011061:Poly A; D010749:Phosphoprotein Phosphatases