title: Hemorrhagic shock and the central vasopressin and opioid peptide system of rats.
abstract: The effect of hemorrhagic shock (40% of blood vol) on the distribution of immunoreactive dynorphin A (Dyn A-IR), [Arg8]vasopressin (AVP-IR), and [Leu5]enkephalin (LE-IR) in the pituitary and brain nuclei was studied in the conscious rat. At 24 h after hemorrhage, the neurointermediate lobe (NIL) showed a reduction in Dyn A-IR (52%) and AVP-IR (32%) and an increase in LE-IR (72%); at this time, the anterior lobe also showed decreased Dyn A-IR (50%) and increased LE-IR (210%). Dyn A-IR, but not LE-IR, was also significantly depleted in some forebrain nuclei in all experimental groups as compared with intact controls, whereas Dyn A-IR in the hypothalamic ventromedial nucleus was elevated only in the sham-control rats. AVP-IR was elevated in the supraoptic nucleus and median eminence (200 and 31%, respectively) 2 and 24 h after bleeding, although plasma AVP returned to normal levels. These data indicate that stress and hypovolemic hypotension produce site and time-dependent change in distribution of dynorphins, AVP, and LE in the central nervous system.
authors.forename: G
authors.initials: G
authors.lastname: Feuerstein
authors.forename: C J
authors.initials: CJ
authors.lastname: Molineaux
authors.forename: J G
authors.initials: JG
authors.lastname: Rosenberger
authors.forename: R L
authors.initials: RL
authors.lastname: Zerbe
authors.forename: B M
authors.initials: BM
authors.lastname: Cox
authors.forename: A I
authors.initials: AI
authors.lastname: Faden
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis* / Q000097:blood; D001794:Blood Pressure; D001923:Brain Chemistry; D004399:Dynorphins / Q000032:analysis*; D004743:Enkephalin, Leucine / Q000032:analysis*; D006339:Heart Rate; D006400:Hematocrit; D008297:Male; D010902:Pituitary Gland / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012771:Shock, Hemorrhagic / Q000503:physiopathology*; D014018:Tissue Distribution
chemical_list: D001127:Arginine Vasopressin; D004743:Enkephalin, Leucine; D004399:Dynorphins