Characterization of a pAM beta 1 deletion derivative isolated from Lactobacillus casei after conjugation.
Streptococcal plasmid pAM beta 1 was conjugally transferred from Streptococcus lactis KB953 (a transformant of pAM beta 1) into Lactobacillus casei 239. A unique transconjugant, L. casei C2, was found to contain a small (11.1 kilobase pair) plasmid, pLY201, which was derived by a deletion event from pAM beta 1. Restriction analysis revealed that pLY201 was missing approximately 58% of the original pAM beta 1 genome, and contained 5 single restriction sites for AvaI, EcoRI, PvuII, HpaI and KpnI. Physical analyses revealed that the stability and copy number of pLY201 were elevated compared with those of pAM beta 1 in L. casei. In addition, pLY201 was no longer transferable by conjugation.
Iwata
M
M
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D007780:Lacticaseibacillus casei / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics*; D010957:Plasmids; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes