Isolation and analysis of the mouse opsin gene.
We have identified three overlapping 5'-truncated mouse opsin cDNA clones by immunologically screening a lambda gt11 retina expression library. Using one of the cDNA clones as a probe, we isolated a 5 kb genomic fragment that encompassed the complete coding sequence for mouse opsin. The coding region for opsin was interrupted by four introns positioned precisely as those previously described for other mammalian opsins. In contrast to the single major opsin mRNA in the bovine and human retina, Northern analysis of mouse retina RNA demonstrated the presence of at least five distinct species of polyadenylated opsin mRNAs. Their sizes ranged from 1.7 kb to 5.1 kb.
Baehr
W
W
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.
Falk
J D
JD
Bugra
K
K
Triantafyllos
J T
JT
McGinnis
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005091:Exons; D005136:Eye Proteins / Q000235:genetics*; D007438:Introns; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells; D012160:Retina / Q000032:analysis; D012168:Retinal Pigments; D017299:Rod Opsins; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D005136:Eye Proteins; D012168:Retinal Pigments; D017299:Rod Opsins; D004247:DNA; D004262:DNA Restriction Enzymes