title: Multilayer-enzyme linked immunosorbent assay (ML-ELISA) for detection of Entamoeba histolytica trophozoite coproantigen.
abstract: Multilayer ELISA (ML-ELISA) method for Entamoeba histolytica trophozoite coproantigen was developed for diagnosis of amoebiasis and its usefulness vis-a-vis microscopic examination analysed for paired specimens from 87 patients from Lucknow and Hyderabad, India. All the stool specimens positive for trophozoites were also found to be positive in ML-ELISA. Out of a total 32 samples positive for cysts in microscopy 21 (65.62%) gave positive reactions for coproantigen. Results on 26 samples drawn from otherwise healthy subjects with negative microscopical picture, 4 were positive for coproantigen. The results of the present study indicate that ML-ELISA test is suitable for early diagnosis of acute amoebic patients for timely treatment.
authors.forename: P
authors.initials: P
authors.lastname: Anand
authors.forename: B
authors.initials: B
authors.lastname: Malaviya
authors.forename: P
authors.initials: P
authors.lastname: Das
authors.forename: M A
authors.initials: MA
authors.lastname: Mateen
authors.forename: C M
authors.initials: CM
authors.lastname: Habibullah
authors.forename: S R
authors.initials: SR
authors.lastname: Das
mesh_terms: D000562:Amebiasis / Q000175:diagnosis*; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D003951:Diagnostic Errors; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000175:diagnosis* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology; D006801:Humans; D011817:Rabbits
chemical_list: D000953:Antigens, Protozoan