The effect of substrate on the production of infectious virus by cells in culture.
Herpes simplex virus type I (HSV-1), infectious bovine rhinotracheitis virus (IBR) and turkey herpesvirus were examined for growth in cells cultured on three different substrates. The substrates were glass, DEAE-dextran and collagen gel. With two of the viruses, HSV-1 and IBR, there were no apparent differences in production as a function of substrate. In contrast, the amount of the turkey herpesvirus which was recovered varied greatly with the substrate. Titers were highest on glass, followed by DEAE-dextran and then collagen gel. Our previous studies have indicated that the substrate on which anchorage-dependent cells are grown in vitro has an affect on a number of biological and biochemical properties. The present study indicates that the production of commercially important biologicals can be affected by the substrate.
Varani
J
J
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109.
Bendelow
M J
MJ
Hillegas
W J
WJ
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen; D003637:DEAE-Dextran; D005898:Glass; D006564:Herpesviridae / Q000254:growth & development; D007242:Herpesvirus 1, Bovine / Q000254:growth & development; D006801:Humans; D018139:Simplexvirus / Q000254:growth & development; D013499:Surface Properties; D014776:Virus Cultivation / Q000295:instrumentation*
D003094:Collagen; D003637:DEAE-Dextran