Cystine-glutamate transport interactions in rat renal cortical tubules, brushborder vesicles, and cultured renal tubule cells.
Glutamate had no significant effect on the uptake of 0.025 mM cystine by isolated rat renal cortical tubules and brushborder membrane vesicles in contrast to lysine which significantly inhibits cystine transport. Glutamate, however, markedly inhibited cystine uptake by rat renal tubule cells grown in a serum-free, hormonally defined media for 5 days. Lysine also inhibited cystine transport in these cultured renal tubule cells.
Foreman
J W
JW
McNamara
P D
PD
Bowring
M A
MA
Lee
J
J
Rea
C
C
Segal
S
S
D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D046508:Culture Techniques; D003553:Cystine / Q000378:metabolism*; D004347:Drug Interactions; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D007672:Kidney Cortex / Q000648:ultrastructure*; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008239:Lysine / Q000494:pharmacology; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D005971:Glutamates; D018698:Glutamic Acid; D003553:Cystine; D008239:Lysine