Ageing of fixed cytological preparations produces degradation of chromosomal DNA.
When fixed chromosome preparations were allowed to age for 1-72 h, they became progressively more susceptible to digestion by exonuclease III and by S1 nuclease. Analysis of DNA from these aged preparations on agarose gels showed that the molecular weight of the DNA decreased as ageing progressed. We conclude that DNA in fixed chromosome preparations becomes progressively degraded as the preparations age.
Mezzanotte
R
R
Istituto di Biologia Generale, Facolta di Medicina e Chirurgia, Universita di Cagliari, Italy.
Vanni
R
R
Flore
O
O
Ferrucci
L
L
Sumner
A T
AT
D002470:Cell Survival; D003584:Cytological Techniques; D004247:DNA / Q000378:metabolism*; D004720:Endonucleases; D005090:Exodeoxyribonucleases; D006801:Humans; D008677:Metaphase; D008970:Molecular Weight; D010610:Pharyngeal Neoplasms; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013997:Time Factors; D014407:Tumor Cells, Cultured
D004247:DNA; D004720:Endonucleases; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D015719:Single-Strand Specific DNA and RNA Endonucleases