title: [Establishment and characterization of a human cholangiocellular carcinoma cell line].
abstract: A cell line, HuH-28 was established in vitro from a patient with cholangiocellular carcinoma (CCC). This cell line has been in continuous culture over 10 month period with slow growth potential. HuH-28 was composed of spindle-shaped cells as major population besides a small percentage of polygonal-shaped cells. Chromosome number of the cells were distributed near the hypotriploid region on the 3rd passage. HuH-28 cells were not transplantable into nude mice, but secreted some tumor markers including alkaline phosphatase (ALP), gamma glutamyltranspeptidase (GGT), beta 2-microglobulin (BMG), ferritin, elastase-1 and tissue polypeptide antigen (TPA). This HuH-28 cell line will represent a good model for the investigation of carcinogenesis, histogenesis+ and diagnosis of CCC.
authors.affiliation: Division of Pathology, Cancer Institute, Medical School, Okayama University.
authors.forename: Y
authors.initials: Y
authors.lastname: Kusaka
authors.forename: A
authors.initials: A
authors.lastname: Muraoka
authors.forename: T
authors.initials: T
authors.lastname: Tokiwa
authors.forename: J
authors.initials: J
authors.lastname: Sato
mesh_terms: D002759:Adenoma, Bile Duct / Q000473:pathology*; D000328:Adult; D000818:Animals; D001650:Bile Duct Neoplasms / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis; D005260:Female; D006801:Humans; D051379:Mice; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor