Lens membrane properties following hydrogen peroxide exposure.
Rabbit lenses exposed to 0.5 mM hydrogen peroxide for 1 h and then incubated for an additional 19 h demonstrated small yet significant changes in electrolyte balance. Active cation pumping by the lens, as determined by 86Rb uptake studies, was partially impaired following 1 h hydrogen peroxide exposure; the cation pump further deteriorated during the subsequent 19-hour incubation. Passive membrane permeability of the lens, measured by 86Rb efflux, was elevated following 1 h hydrogen peroxide but no further increase in leakiness was observed during the subsequent 19-hour incubation. These findings suggest that even relatively brief exposure to oxidative damage initiates a chain of events from which the lens is unable to recover.
Delamere
N A
NA
Department of Ophthalmology, University of Louisville, Ky.
Paterson
C A
CA
Hensley
S
S
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002463:Cell Membrane Permeability; D003470:Culture Media; D046508:Culture Techniques; D004573:Electrolytes / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism; D012413:Rubidium / Q000378:metabolism; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D004573:Electrolytes; D015221:Potassium Channels; D015222:Sodium Channels; D006861:Hydrogen Peroxide; D012413:Rubidium