Identification of microtubule-associated proteins (MAPs) in Xenopus oocyte.
Microtubules were isolated from prophase-blocked oocytes of Xenopus laevis with the use of the anti-tumor drug taxol. In addition to tubulin, 5 microtubule-associated proteins (MAPs) were characterized. Among them, 2 high molecular mass proteins (200-300 kDa) are phosphorylated in ovo. The oocyte MAP extract promotes the assembly of rat brain 6 S purified tubulin.
Jessus
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Thibier
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Ozon
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D000470:Alkaloids; D000818:Animals; D002458:Cell Fractionation; D005260:Female; D006358:Hot Temperature; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D008870:Microtubules / Q000032:analysis; D008970:Molecular Weight; D009865:Oocytes / Q000032:analysis* / Q000378:metabolism; D017239:Paclitaxel; D010766:Phosphorylation; D051381:Rats; D014404:Tubulin / Q000378:metabolism; D014982:Xenopus laevis
D000470:Alkaloids; D008869:Microtubule-Associated Proteins; D014404:Tubulin; D017239:Paclitaxel