Constitutive expression of HLA class II antigens on EBV positive malignant cells from nasopharyngeal carcinoma: possible involvement in T cell infiltration.
Undifferentiated nasopharyngeal carcinoma (NPC) was originally referred to as "lymphoepithelial carcinoma." Two main cell types are constantly associated within these tumors: the malignant epithelial cells which harbor the Epstein-Barr virus (EBV) genome, and the nonmalignant lymphocytes mostly of the T lineage. Three characteristic features of malignant NPC cells might explain T cell migration within NPC tumor tissue: HLA class II molecule expression, IL-1 production, and presence of EBV antigens. Homogeneous suspensions of malignant NPC cells were derived from a nude-mouse transplantable tumor in order to specify the status of HLA class II molecules in these tumors. These suspensions were stained for HLA class II antigens and analyzed by flow cytometry in comparison with other carcinoma cell lines. Three characteristics of transplantable malignant NPC cells were demonstrated: constitutive and coordinate expression of DR, DP, and DQ molecules, and concomitant expression with the CDw40 antigen ("B lymphocyte carcinoma cross-reacting antigen").
Busson
P
P
Laboratoire d'Immunobiologie des Tumeurs, Institut Gustave Roussy, Villejuif, France.
Braham
K
K
Clausse
B
B
Tursz
T
T
D000293:Adolescent; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D005260:Female; D005434:Flow Cytometry; D006680:HLA Antigens / Q000032:analysis; D004854:Herpesvirus 4, Human; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D009303:Nasopharyngeal Neoplasms / Q000276:immunology* / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology*; D014412:Tumor Virus Infections
D000945:Antigens, Differentiation, T-Lymphocyte; D000956:Antigens, Viral; D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1