title: Fimbriation and P-antigen recognition of Escherichia coli strains harbouring mutated recombinant plasmids encoding fimbrial adhesins of the uropathogenic E. coli strain KS71.
abstract: Deletion mutants of recombinant plasmids encoding the KS71B fimbrial antigens of the uropathogenic Escherichia coli strain KS71 (O4:K12) were constructed. The effects of these mutations were tested by transforming the mutated plasmids into non-fimbriated E. coli HB101 cells and testing the transformants for fimbriation and haemagglutination. A deletion transcriptionally upstream from the fimbrial subunit gene increased the expression of KS71B fimbriae. Deletion of the fimbrial subunit gene resulted in non-fimbriated but haemagglutinating transformants, whereas a deletion 6 kb transcriptionally downstream from the subunit gene resulted in non-haemagglutinating but fimbriate transformants, indicating that fimbriation and haemagglutination were genetically separable. We also present evidence suggesting that the fimbrillin and haemagglutinin are physically associated in the wild-type KS71 strain.
authors.forename: M
authors.initials: M
authors.lastname: Rhen
authors.forename: J
authors.initials: J
authors.lastname: Tenhunen
authors.forename: V
authors.initials: V
authors.lastname: Väisänen-Rhen
authors.forename: A
authors.initials: A
authors.lastname: Pere
authors.forename: M
authors.initials: M
authors.lastname: Båga
authors.forename: T K
authors.initials: TK
authors.lastname: Korhonen
mesh_terms: D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000276:immunology*; D005798:Genes, Bacterial; D006384:Hemagglutination; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D009154:Mutation; D010957:Plasmids
chemical_list: D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004274:DNA, Recombinant; D007140:Immunoglobulin Fab Fragments; D004262:DNA Restriction Enzymes