title: Adrenergic control of lipogenesis and lipolysis in the chicken in vitro.
abstract: The adrenergic inhibition of lipogenesis and stimulation of lipolysis in the avian has been examined using chicken hepatocytes and adipose tissue explants in vitro. Lipogenesis was inhibited by adrenergic agonists: epinephrine (alpha + beta) greater than isoproterenol (beta 1/beta 2) greater than norepinephrine (alpha 1/alpha 2, beta 1) greater than metaproterenol (beta 2), phenylephrine (alpha 1). Dobutamine (beta 1 agonist) and dopamine (dopaminergic agonist) did not significantly affect [14C]acetate incorporation into lipid, while clonidine and para-aminoclonidine (alpha 2 agonists) were slightly stimulatory. Lipolysis in young and adult chicken adipose tissue was stimulated by epinephrine, isoproterenol, phenylephrine, dobutamine and metaproterenol, but was inhibited by clonidine and para-aminoclonidine. Both the antilipogenic and lipolytic effects of epinephrine were partially blocked by phentolamine (alpha 1 = alpha 2 antagonist) or propranolol (beta 1 = beta 2 antagonist), but completely inhibited by phentolamine and propranolol administered together.
authors.forename: R M
authors.initials: RM
authors.lastname: Campbell
authors.forename: C G
authors.initials: CG
authors.lastname: Scanes
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008053:Lipid Mobilization / Q000187:drug effects; D008099:Liver / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000502:physiology*; D013565:Sympatholytics / Q000494:pharmacology; D013566:Sympathomimetics / Q000494:pharmacology
chemical_list: D013565:Sympatholytics; D013566:Sympathomimetics