[The effect of reversed micelles of cetyltrimethylammonium bromide on equilibrium constants and kinetics of oxidation-reduction reactions with the participation of cytochrome c].
To model the effect of membrane environment on the electron transfer reactions we studied the thermodynamics and kinetics of the reactions of cytochrome c and 2,6-dichlorophenolindophenol with ferri- and ferrocyanide in the reversed micelles cetyltrimethylammonium bromide in chloroform/octan mixture. Incorporation of the protein in micelles leads to increasing the equilibrium constant (K1) up to 300 times. This effect is mainly due to a decrease in the ferrocytochrome c oxidation rate constant in the reaction with ferricyanide. Micellar solubilization of the dye also leads to a marked increase in the equilibrium constant K2. The estimations of the values K1 and K2 in water-alcohol mixtures and in aqueous micellar solutions of surfactant together with kinetical and spectral data show that the increase of K1 and K2 in reversed micelles is caused generally by redox potential changes of low-molecular reagents. The latter change their environment after adsorption on the micellar surface.
Iushchishina
A N
AN
Genkin
M V
MV
Koroteev
S V
SV
Malievskiĭ
A D
AD
Davydov
R M
RM
D015086:2,6-Dichloroindophenol; D000818:Animals; D002593:Cetrimonium Compounds; D055598:Chemical Phenomena; D002621:Chemistry; D003574:Cytochrome c Group / Q000378:metabolism*; D004579:Electron Transport; D005292:Ferricyanides; D005295:Ferrocyanides; D006736:Horses; D007202:Indicators and Reagents; D007700:Kinetics; D008823:Micelles; D009206:Myocardium / Q000201:enzymology; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds
D002593:Cetrimonium Compounds; D003574:Cytochrome c Group; D005292:Ferricyanides; D005295:Ferrocyanides; D007202:Indicators and Reagents; D008823:Micelles; D000644:Quaternary Ammonium Compounds; D015086:2,6-Dichloroindophenol
Vliianie obrashchennykh mitsell bromistogo tsetiltrimetilammoniia na konstanty ravnovesiia i kinetiku okislitel'no-vosstanovitelnykh reaktsiĭ s uchastiem tsitokhrom c.