DNA damage induced in mammalian cells by active oxygen species.
The active oxygen species generated by ionizing radiation, hyperoxia, paraquat and hydrogen peroxide induced unscheduled DNA synthesis and DNA synthesis rate inhibition in human fetal lung fibroblast (IMR-90) and transformed cell line derived from Syrian hamster embryo fibroblast (BP6T). The D-glucose and sucrose, which were unable to generate active oxygen species, could induce neither unscheduled DNA synthesis nor DNA synthesis rate inhibition. The indicator of DNA synthesis rate inhibition for active oxygen species was more sensitive than that of unscheduled DNA synthesis. All the enhancing chemicals on active oxygen species aggravated DNA damage, while all the inhibiting chemicals alleviated DNA damage. Results showed that active oxygen species do damage DNA. The active oxygen species mechanism for carcinogenesis, mutation and aging is applicable.
Zheng
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RL
Division of Biophysics, School of Hygiene & Public Health, Johns Hopkins University, Baltimore.
Lesko
S A
SA
Ts'o
P O
PO
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis* / Q000528:radiation effects; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000528:radiation effects; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology; D005720:Gamma Rays; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008647:Mesocricetus; D010100:Oxygen / Q000633:toxicity*; D010269:Paraquat / Q000494:pharmacology; D013481:Superoxides / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism
D004273:DNA, Neoplasm; D013481:Superoxides; D004247:DNA; D006861:Hydrogen Peroxide; D010269:Paraquat; D010100:Oxygen