31P NMR measurement of ATP synthesis rate in perfused intact rat hearts.
Using 31P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 +/- 2.5 (SE) X 10(3) mmHg X min-1 and consuming O2 at a rate of 35 +/- 2 mumol O2 X min-1 X (g dry wt)-1, at 37 degrees C. This rate was 7.2 +/- 0.9 mumol X s-1 X (g dry wt)-1 and was related to the rate of oxygen atom consumption by a ratio of 6.3 +/- 0.9. These data show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.
Kingsley-Hickman
P
P
Sako
E Y
EY
Andreone
P A
PA
St Cyr
J A
JA
Michurski
S
S
Foker
J E
JE
From
A H
AH
Petein
M
M
Ugurbil
K
K
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010101:Oxygen Consumption; D010477:Perfusion; D010710:Phosphates / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000032:analysis; D051381:Rats
D010710:Phosphates; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases