title: Effects of hydroxyl radical scavengers KCN and CO on ultraviolet light-induced activation of crude soluble guanylate cyclase.
abstract: The crude soluble guanylate cyclase (GC) from bovine mesenteric artery was stimulated by ultraviolet (UV) light (366 nm). Addition of free radical scavengers, dimethylsulfoxide or superoxide dismutase and/or catalase to the GC assay did not abolish the stimulatory effect of UV light. On the contrary, the UV light-induced activation was enhanced in the presence of these scavengers. KCN (1 mM) did not affect the UV light-induced activation, while 0.1 mM of CO potentiated the activation. These results may indicate that UV light is operating through a direct interaction with the ferrous form of the GC-heme.
authors.forename: J O
authors.initials: JO
authors.lastname: Karlsson
authors.forename: K L
authors.initials: KL
authors.lastname: Axelsson
authors.forename: R G
authors.initials: RG
authors.lastname: Andersson
mesh_terms: D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D006162:Guanylate Cyclase / Q000378:metabolism*; D006420:Hemeproteins / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008638:Mesenteric Arteries / Q000201:enzymology; D011190:Potassium Cyanide / Q000494:pharmacology; D012995:Solubility; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D006420:Hemeproteins; D002248:Carbon Monoxide; D002374:Catalase; D013482:Superoxide Dismutase; D006162:Guanylate Cyclase; D011190:Potassium Cyanide