Monoenoic fatty acid requirement in V79 cells.
When lipids were removed from the culture medium, growth of V79 cells ceased. Supplementation with cis-octadecenoic acids satisfied the requirement for lipids by V79 cells. After starvation of the exogenous lipids by the shift-down of the medium to lipid-free medium, the content of octadecenoic acid in phospholipids increased more slowly in V79 cells than in V79-R cells, which can grow in the lipid-starved medium. The incorporation of [14C]acetic acid into monoenoic fatty acids and phospholipid molecular species containing monoenoic fatty acids in V79 cells was lower than that in V79-R cells. The reduced formation of monoenoic fatty acids was shown to be due to deficiency in the stimulation of activity of stearoyl-CoA desaturase which is a key enzyme to convert saturated fatty acids to monoenoic fatty acids.
Urade
R
R
Hayashi
Y
Y
Kito
M
M
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D008168:Lung; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism
D000085:Acetates; D003470:Culture Media; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009829:Oleic Acids; D010743:Phospholipids; D019301:Oleic Acid; D013230:Stearoyl-CoA Desaturase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase; D019342:Acetic Acid