title: Properties of light and heavy vesicles simultaneously prepared from hog gastric mucosae.
abstract: We obtained two kinds of vesicle preparations which were of different density from the same gastric mucosae of hogs stimulated with food before slaughter. Both kinds contained H+,K+-ATPase. The light vesicle preparation differed from the heavy vesicle preparation as follows: the KCl permeability across the membrane of heavy vesicles was larger than that of light vesicles, the actin (46-kDa peptide on SDS-polyacrylamide gel) content of heavy vesicles was much higher than that of light vesicles, and the H+,K+-ATPase activity of heavy vesicles was less sensitive to a monoclonal antibody raised against light vesicles (HK2032) than that of light vesicles. Furthermore, there was a drastic difference in reactivity to SCH 28080, which is an H+,K+-ATPase-specific inhibitor and reacts competitively with the K+-high affinity site. SCH 28080 is more potent in light vesicles than in heavy vesicles. These results suggest that the conformation of H+,K+-ATPase changed during the translocation from tubulovesicles to the apical plasma membrane. On the other hand, H+,K+-ATPase activities in both vesicles had similar pH and [K+] dependences.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University.
authors.forename: S
authors.initials: S
authors.lastname: Asano
authors.forename: T
authors.initials: T
authors.lastname: Iino
authors.forename: Y
authors.initials: Y
authors.lastname: Tabuchi
authors.forename: N
authors.initials: N
authors.lastname: Takeguchi
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000032:analysis / Q000201:enzymology / Q000648:ultrastructure*; D017506:H(+)-K(+)-Exchanging ATPase; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology; D007120:Immunochemistry; D009853:Omeprazole / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D013347:Subcellular Fractions / Q000032:analysis* / Q000201:enzymology; D013552:Swine; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D007093:Imidazoles; C035235:Sch 28080; D014634:Valinomycin; D011189:Potassium Chloride; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase; D009853:Omeprazole