Determination of fat-soluble vitamins in a pharmaceutical dosage form by solid-phase extraction and reversed-phase liquid chromatography.
A rapid and precise method for the determination of fat-soluble vitamins from a water-based multivitamin mixture was developed utilizing solid-phase extraction and reversed-phase high-performance liquid chromatography (HPLC). Cholecalciferol, alpha-tocopherol acetate, and retinol palmitate were extracted in a single stage from the matrix using Bond Elut C18 columns. The vitamins were then chromatographed on a Hypersil 5-microns C18 column, using a water:methanol gradient, and quantified simultaneously using individual UV absorption maxima. The recovery of added analytes varied from 92.6 to 100.6%. The assay coefficient of variation was 1.7-2.7%. The sample preparation method and HPLC assay described here are practical for pharmaceutical quality control purposes.
Savolainen
K E
KE
Orion Corporation, Ltd., Orion Pharmaceutica, Kuopio, Finland.
Pynnönen
K M
KM
Lapinjoki
S P
SP
Vidgren
M T
MT
D002762:Cholecalciferol / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D004304:Dosage Forms; D000084562:Retinyl Esters; D013056:Spectrophotometry, Ultraviolet; D014801:Vitamin A / Q000031:analogs & derivatives / Q000032:analysis; D014810:Vitamin E / Q000032:analysis; D014815:Vitamins / Q000008:administration & dosage / Q000032:analysis*
D004224:Diterpenes; D004304:Dosage Forms; D000084562:Retinyl Esters; D014815:Vitamins; D014801:Vitamin A; D014810:Vitamin E; D002762:Cholecalciferol; C014794:retinol palmitate