title: Expression of oncogenes in human hepatoma cell lines.
abstract: The expression of 20 known cellular proto-oncogenes in human well-differentiated hepatoma cell line Hep3B and poorly-differentiated hepatoma cell line HA22T/VGH was studied by Northern blot hybridization. Among the cellular proto-oncogenes examined, both cell lines express protein kinase genes including fps, mos and raf; PDGF B chain sis gene; GTP/GDP binding protein gene Ha-ras and nuclear protein genes including fos and myc. The expression of yes, abl, ros, src, erb-B, erb-A, fms, Ki-ras, myb, rel and bas genes was not detected in both cell lines.
authors.affiliation: Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei, Taiwan, ROC.
authors.forename: L P
authors.initials: LP
authors.lastname: Ting
authors.forename: K S
authors.initials: KS
authors.lastname: Jeng
authors.forename: C K
authors.initials: CK
authors.lastname: Chou
authors.forename: T S
authors.initials: TS
authors.lastname: Su
authors.forename: C P
authors.initials: CP
authors.lastname: Hu
authors.forename: F H
authors.initials: FH
authors.lastname: Wong
authors.forename: H K
authors.initials: HK
authors.lastname: Chang
authors.forename: C M
authors.initials: CM
authors.lastname: Chang
mesh_terms: D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation; D002460:Cell Line; D015342:DNA Probes; D019204:GTP-Binding Proteins / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020574:Proto-Oncogene Proteins c-sis; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000032:analysis
chemical_list: D015342:DNA Probes; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D012334:RNA, Neoplasm; D011494:Protein Kinases; D019204:GTP-Binding Proteins