[Optimization of the growth of anaerobic bacteria by addition of a perfluorinated oil].
Strict anaerobic bacteria require special conditions of culture; their obtention in the laboratory therefore is difficult especially for poor inocula. A method for stimulating anaerobes growth is described: a perfluorinated oil in which CO2 has been dissolved is added to the classical culture medium. The latency phase of the bacterial growth is strongly decreased especially for poor inocula. Whatever the inoculum and the gas bubbling time are, the rate of growth remains constant. The changes in bubbling times do not induce significant differences in latency time. This technique has been also applied to the starting of lyophilized strains.
Ceschin
C
C
Malet-Martino
M C
MC
Michel
G
G
Lattes
A
A
D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000254:growth & development*; D001439:Bacteroides / Q000254:growth & development; D002245:Carbon Dioxide / Q000494:pharmacology; D003016:Clostridium perfringens / Q000254:growth & development; D003470:Culture Media; D005466:Fluorocarbons / Q000494:pharmacology*; D005612:Freeze Drying; D005673:Fusobacterium / Q000254:growth & development; D012995:Solubility; D013268:Stimulation, Chemical; D013997:Time Factors
D003470:Culture Media; D005466:Fluorocarbons; D002245:Carbon Dioxide; C046505:bis(perfluoro-n-hexyl)-1,2-ethylene
Optimisation de la croissance des bactéries anaérobies par addition d'une huile perfluorée.