title: In vivo protein synthesis in different tissues and the whole body of rainbow trout (Salmo gairdnerii R.). Influence of environmental temperature.
abstract: The fractional protein synthesis rate (FSR) of tissue (liver, digestive tract, muscle and whole fish) proteins was measured in rainbow trout acclimated to 9 and 18 degrees C after a pulse injection of [U-14C] L-leucine. In each of the tissues two FSRs were calculated based on a different estimate of the specific radioactivity of leucine in the precursor compartment for protein synthesis. Whole fish protein synthesis (WFPS) was estimated to be 7 and 7.6 g protein per kg body weight and per day respectively at 10 and 18 degrees C. Muscle and digestive tract contributed the most (more than 30%) to WFPS. The rate of protein turnover in whole fish was very low, as in the muscle, when compared to liver and digestive tract.
authors.forename: B
authors.initials: B
authors.lastname: Fauconneau
authors.forename: M
authors.initials: M
authors.lastname: Arnal
mesh_terms: D000064:Acclimatization; D000818:Animals; D002250:Carbon Radioisotopes; D004032:Diet; D004064:Digestive System / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009928:Organ Specificity; D014176:Protein Biosynthesis; D012487:Salmonidae / Q000378:metabolism*; D013696:Temperature; D014337:Trout / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D007930:Leucine