Rearrangement of the T-cell-receptor beta-chain gene in the diagnosis of lymphoproliferative disorders.
The arrangement of the T-cell-receptor and immunoglobulin genes has been analysed in 77 cases of lymphoproliferative disorder. All 6 T-cell leukaemias and 16 of 19 T-cell lymphomas showed rearrangement of the gene coding for the beta chain of the T-cell receptor, associated in all cases with a germline arrangement of the immunoglobulin genes. All 36 B-cell leukaemias and all 16 B-cell lymphomas showed rearrangement of immunoglobulin genes; the T-cell-receptor gene was in the germline configuration in most of these cases but showed a rearranged pattern in 3 cases (2 chronic lymphatic leukaemias and 1 immunoblastic lymphoma). The combined use of T-cell-receptor and immunoglobulin gene probes promises to be a valuable means of identifying and classifying T-cell neoplasms.
O'Connor
N T
NT
Wainscoat
J S
JS
Weatherall
D J
DJ
Gatter
K C
KC
Feller
A C
AC
Isaacson
P
P
Jones
D
D
Lennert
K
K
Pallesen
G
G
Ramsey
A
A
D001345:Autoradiography; D001402:B-Lymphocytes; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D007938:Leukemia / Q000175:diagnosis* / Q000235:genetics; D008223:Lymphoma / Q000175:diagnosis* / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes
D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell; D004247:DNA; D004262:DNA Restriction Enzymes