Analysis of hsp 30, hsp 70 and ubiquitin gene expression in Xenopus laevis tadpoles.
Heat-induced accumulation of hsp 30 mRNA (1.1 kb) during early development of Xenopus laevis was first detectable at the tailbud stage (stage 30-34). This contrasts with heat-induced accumulation of hsp 70 mRNA (2.7 kb) and ubiquitin mRNA (size range = 1.7-3.1 kb), which was first detectable at the mid- to late-blastula stage. Continuous exposure of tadpoles to a 33 degrees C heat shock resulted in a coordinate, transient accumulation of hsp 30, hsp 70 and ubiquitin mRNA. A coordinate, temporal pattern was also observed for the decay of hsp 30, hsp 70 and ubiquitin mRNA in tadpoles recovering at 22 degrees C following a 1 h heat shock at 33 degrees C. Thus, while hsp 30 genes are regulated differently during development compared with hsp 70 and ubiquitin genes, these genes all exhibit a coordinate heat-inducible pattern of expression at the tadpole stage. Levels of alpha-cardiac actin mRNA remained unchanged during continuous heat shock and recovery experiments.
Krone
P H
PH
Department of Biology, University of Waterloo, Ontario, Canada.
Heikkila
J J
JJ
D000199:Actins / Q000378:metabolism; D000818:Animals; D004587:Electrophoresis, Agar Gel; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis; D014452:Ubiquitins / Q000235:genetics*; D014982:Xenopus laevis / Q000196:embryology*
D000199:Actins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D014452:Ubiquitins