title: Participation of mitochondrial proliferation in morphological differentiation of murine mastocytoma cells.
abstract: Proliferation of a cold-sensitive cell-cycle mutant isolated from an undifferentiated murine mastocytoma line is reversibly arrested at the nonpermissive temperature of 33 degrees C, and the arrested cells undergo morphological differentiation as expressed by the formation of metachromatic granules. Following transfer of these mutant cells from the permissive temperature of 39.5 to 33 degrees C, a transient increase in both cytochrome c oxidase and DNA polymerase gamma was observed, the ratio of total mitochondrial volume to cell volume nearly doubled within 6 days, and numbers of mitochondrial cross-sections per cellular cross-section as determined in electron micrographs underwent a threefold increase. Addition of chloramphenicol (100 micrograms/ml) to the mutant cell cultures 6 days prior to transfer from 39.5 to 33 degrees C prevented the increase in the ratio of total mitochondrial to cell volume. Furthermore, chloramphenicol markedly inhibited the increase in granule number per cell that normally is observed after transfer of cultures to 33 degrees C or during treatment with 1 mM butyrate, suggesting that mitochondrial proliferation may be an obligatory step in the process of morphological differentiation of these mastocytoma cells.
authors.affiliation: Department of Pathology, University of Bern, Switzerland.
authors.forename: H
authors.initials: H
authors.lastname: Laeng
authors.forename: E
authors.initials: E
authors.lastname: Schneider
authors.forename: R
authors.initials: R
authors.lastname: Bolli
authors.forename: A
authors.initials: A
authors.lastname: Zimmermann
authors.forename: T
authors.initials: T
authors.lastname: Schaffner
authors.forename: R
authors.initials: R
authors.lastname: Schindler
mesh_terms: D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002701:Chloramphenicol / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000378:metabolism; D012515:Mast-Cell Sarcoma / Q000201:enzymology / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D013696:Temperature
chemical_list: D002087:Butyrates; D020148:Butyric Acid; D002701:Chloramphenicol; D003576:Electron Transport Complex IV