title: Characteristics of the translation of thymosin alpha 1 precursor mRNA by cell-free wheat germ system. Evidence for the acetylation of thymosin alpha 1 precursor.
abstract: At the optimal concentrations of Mg2+ and K+ to translate total thymus PolyA+-RNA, the purified thymosin alpha 1 precursor mRNA saturate the protein synthetic activity at lower concentration than the total thymus mRNAs. The polyamine spermidine increases the translation rate of the messenger, which is modulated by magnesium, rather than improve the yield in full-length chains of thymosin alpha 1 precursor. As other eukariotic mRNAs, this messenger presents the "cap" modification at the 5'-end terminal position. The incorporation of [3H]acetate into the translation product of the messenger, shows an evidence for the acetylation of the thymosin alpha 1 precursor during its biosynthesis in vitro.
authors.forename: M
authors.initials: M
authors.lastname: Nogueira
authors.forename: M
authors.initials: M
authors.lastname: Freire
mesh_terms: D000107:Acetylation; D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D008274:Magnesium / Q000494:pharmacology; D011061:Poly A / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D011498:Protein Precursors / Q000235:genetics*; D012315:RNA Caps / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000235:genetics; D013950:Thymus Gland / Q000032:analysis; D014908:Triticum
chemical_list: D011498:Protein Precursors; D012315:RNA Caps; D012333:RNA, Messenger; D011061:Poly A; D013947:Thymosin; D008274:Magnesium; D011188:Potassium; D013095:Spermidine; D000077596:Thymalfasin