Effect of dietary selenium and injected gold thioglucose on adjuvant-treated rats.
Gold (Au) thioglucose, which has been used in the treatment of rheumatoid arthritis, inhibits selenium (Se)-glutathione peroxidase. Since Au and Se play roles in inflammation, the effects of dietary Se (0, 0.2, and 2.0 ppm for 10 weeks) and injected gold thioglucose (5 mg Au/day/kg body weight for 28 days) in adjuvant-treated rats were investigated. Au toxicity was evidenced by lower body weights and higher tissue weight/body weight ratios for kidneys and spleens of Au-treated rats. Adjuvant-induced inflammation, measured by paw thickness, was not influenced by dietary Se, although Au decreased inflammation in Se-deficient rats. Liver glutathione peroxidase activity was depressed by Se deficiency and by Au. Sulfhydryl levels in liver soluble fraction and plasma were highest for Se-deficient rats. Among liver, kidney, spleen, and plasma, thiobarbituric acid reactants were highest in kidneys of Au-treated rats and lowest in plasma of rats fed 2 ppm Se. gamma-Glutamyltranspeptidase activity in plasma indicated liver damage in Se-deficient rats. Kidney PGE2 output in 24-hour urine samples was unaffected by Au, Se, or adjuvant. Au-Se interactions in vivo are complex, but decreased glutathione peroxidase activity in Au-injected rats suggests that Se nutrition of Au-treated rheumatoid arthritis patients may be a practical concern.
Dillard
C J
CJ
Baker
M A
MA
Gavino
V C
VC
Tappel
A
A
Tappel
A L
AL
D000818:Animals; D006051:Aurothioglucose / Q000627:therapeutic use*; D001835:Body Weight; D004032:Diet; D015232:Dinoprostone; D005620:Freund's Adjuvant; D005979:Glutathione Peroxidase / Q000378:metabolism; D006046:Gold / Q000627:therapeutic use*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis / Q000201:enzymology; D008297:Male; D009929:Organ Size; D011458:Prostaglandins E / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000627:therapeutic use*; D013858:Thiobarbiturates; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D011458:Prostaglandins E; D013858:Thiobarbiturates; D006051:Aurothioglucose; D006046:Gold; D005620:Freund's Adjuvant; D005979:Glutathione Peroxidase; D005723:gamma-Glutamyltransferase; D012643:Selenium; D015232:Dinoprostone; C029684:thiobarbituric acid