Biogenesis and cell cycle relationship of poly(A)- actin mRNA in mouse ascites cells.
A variety of rapidly growing mammalian cells contain a substantial portion of their actin mRNA in a poly(A)- form. We have used DNA-driven hybridization of a cloned actin cDNA-containing plasmid with pulse-labeled RNA from mouse S-180 ascites cells to examine newly synthesized actin mRNA. Our results indicate that the same proportion of newly synthesized and steady-state actin mRNA (approx. 40%) exists in a poly(A)- deficient form. This suggests that the poly(A)- form arises by some process other than slow cytoplasmic de-adenylation of a poly(A)+ precursor. We have also examined cell cycle-enriched populations of S-180 ascites cells for the presence of poly(A)- actin mRNA. Results from these experiments indicate that cells in G1 phase of the cell cycle contain predominantly poly(A)+ actin mRNA, while the poly(A)- form is restricted to late-S and post-S phase cells.
Geoghegan
T E
TE
McCoy
L
L
D000199:Actins / Q000235:genetics*; D000818:Animals; D001202:Ascitic Fluid / Q000473:pathology; D002478:Cells, Cultured; D004247:DNA; D007399:Interphase; D051379:Mice; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D000199:Actins; D012333:RNA, Messenger; D004247:DNA