Studies on the interaction of Clostridium perfringens sialidase with sialic acid linked to the internal galactose in monosialogangliotetraosyl ceramide.
Investigation of the action of highly purified Clostridium perfringens sialidase on ganglioside II3Neu5Ac-Gg4Cer and its oligosaccharide II3Neu5Ac-Gg4, in the presence and absence of sodium cholate, extend earlier results obtained with impure enzyme fractions. Sialidase labeled with 125I was found to bind to various ganglioside substrate micelles, including II3Neu5Ac-Gg4Cer, and to mixed ganglioside-sodium cholate micelles. No binding occurred between the enzyme and the ganglioside-derived oligosaccharide II3Neu5Ac-Gg4, even when radioactive II3Neu5Ac-Gg4-[3H]ol was used. The binding of sialidase to micellar substrate is a condition for enzymic hydrolysis. Correspondingly, II3Neu5Ac-Gg4Cer and II3Neu5Ac-Gg4Cer-sodium cholate micelles were hydrolyzed by the enzyme but II3Neu5Ac-Gg4 was not. Ganglioside oligosaccharide analogues containing an amino function at the reducing terminus or between two oligosaccharide chains, II3Neu5Ac-Gg4-NH2 and (II3Neu5Ac-Gg4)2NH, were hydrolyzed in the absence of cholate. A synthetic analogue of II3Neu5Ac-Gg4Cer containing only the fatty acid moiety and not the sphingosine residue (I1-deoxy-I1-stearamido-II3-monosialo-gangliotetraitol ) behaved as the ganglioside in the presence and absence of sodium cholate.
Corfield
A P
AP
Schauer
R
R
Dorland
L
L
Vliegenthart
J F
JF
Wiegandt
H
H
D055598:Chemical Phenomena; D002621:Chemistry; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D002850:Chromatography, Gel; D003016:Clostridium perfringens / Q000201:enzymology*; D005677:G(M1) Ganglioside / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism*; D011485:Protein Binding; D012794:Sialic Acids / Q000378:metabolism*; D013379:Substrate Specificity; D013997:Time Factors
D002793:Cholic Acids; D005732:Gangliosides; D012794:Sialic Acids; D005677:G(M1) Ganglioside; D009439:Neuraminidase; D019826:Cholic Acid; D019158:N-Acetylneuraminic Acid; D005690:Galactose