Subcellular distribution of neuropeptide Y-like immunoreactivity in guinea pig neocortex.
Neuropeptide Y-like immunoreactivity (NPY-LI) was enriched in synaptosomal fractions of neocortex, which on lysis yielded vesicle-rich fractions. The distribution of NPY-LI on a sucrose density gradient was similar to that of somatostatin, with a concentration in heavy vesicles. The peptides were not found in light vesicles in contrast to the distribution of noradrenaline. Both homogenate and vesicular NPY-LI coeluted with synthetic NPY on reverse-phase HPLC.
de Quidt
M E
ME
Richardson
P J
PJ
Emson
P C
PC
D015720:5'-Nucleotidase; D000818:Animals; D002540:Cerebral Cortex / Q000032:analysis* / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005649:Fumarate Hydratase / Q000378:metabolism; D006168:Guinea Pigs; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009478:Neuropeptide Y; D009638:Norepinephrine / Q000032:analysis; D009708:Nucleotidases / Q000378:metabolism; D013004:Somatostatin / Q000032:analysis; D013574:Synaptosomes / Q000032:analysis
D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D013004:Somatostatin; D007770:L-Lactate Dehydrogenase; D009708:Nucleotidases; D015720:5'-Nucleotidase; D005649:Fumarate Hydratase; D009638:Norepinephrine