N-cadherin and integrins: two receptor systems that mediate neuronal process outgrowth on astrocyte surfaces.
Receptor-mediated interactions between neurons and astroglia are likely to play a crucial role in the growth and guidance of CNS axons. Using antibodies to neuronal cell surface proteins, we identified two receptor systems mediating neurite outgrowth on cultured astrocytes. N-cadherin, a Ca2(+)-dependent cell adhesion molecule, functions prominently in the outgrowth of neurites on astrocytes by E8 and E14 chick ciliary ganglion (CG) neurons. beta 1-class integrin ECM receptor heterodimers function less prominently in E8 and not at all in E14 neurite outgrowth on astrocytes. The lack of effect of integrin beta 1 antibodies on E14 neurite outgrowth reflects an apparent loss of integrin function, as assayed by E14 neuronal attachment and process outgrowth on laminin. N-CAM appeared not to be required for neurite outgrowth by either E8 or E14 neurons. Since N-cadherin and integrin beta 1 antibodies together virtually eliminated E8 CG neurite outgrowth on cultured astrocytes, these two neuronal receptors are probably important in regulating axon growth on astroglia in vivo.
Tomaselli
K J
KJ
Department of Physiology, University of California, San Francisco 94143-0724.
Neugebauer
K M
KM
Bixby
J L
JL
Lilien
J
J
Reichardt
L F
LF
D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015820:Cadherins / Q000276:immunology / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D002645:Chickens; D003712:Dendrites / Q000187:drug effects; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000187:drug effects; D007106:Immune Sera / Q000494:pharmacology; D016023:Integrins / Q000276:immunology / Q000502:physiology*; D009414:Nerve Growth Factors / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor
D015820:Cadherins; D007106:Immune Sera; D016023:Integrins; D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor