Action potentials of cardiac muscle in healing infarcts: response to norepinephrine and caffeine.
Previous studies have shown that the muscle fibers that survive on the epicardial surface of a 5- or 14-day-old infarct in canine hearts have reduced or absent plateau phase during repolarization. Since the absence of a plateau phase could be related to a decrease in the slow inward current, we determined whether norepinephrine (NE) (3.13 x 10(-5) M) or caffeine (2.5 to 5.0 mM) restored the reduced plateau phase of action potentials of fibers from the 5- to 14-day infarct (4 mM K+). In addition, we compared the slow inward current-mediated slow response action potentials induced by NE or caffeine in K+ depolarized fibers from the 5- and 14-day infarcts to those induced in control fibers. Our results show that NE and caffeine had little or no significant effects on repolarization or the plateau area of action potentials of fibers from infarcted preparations. In addition, NE and caffeine both induced slow response action potentials in normal fibers, but in some fibers from infarcts, these agents failed to elicit slow responses. In other fibers from infarcts, NE and caffeine induced slow response action potentials but they had reduced mean areas when compared to the control potentials. In conclusion, the alteration and absence of slow responses in fibers from infarcted preparations suggest that there may be a chronic abnormality in the slow Ca current channel in fibers from the 5- and 14-day infarct.
Boyden
P A
PA
Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Gardner
P I
PI
Wit
A L
AL
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D000242:Cyclic AMP / Q000032:analysis; D004285:Dogs; D006321:Heart / Q000187:drug effects / Q000503:physiopathology*; D007552:Isotonic Solutions / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009203:Myocardial Infarction / Q000503:physiopathology*; D009206:Myocardium / Q000032:analysis; D009638:Norepinephrine / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D013997:Time Factors
D007552:Isotonic Solutions; C037692:Tyrode's solution; D002110:Caffeine; D000242:Cyclic AMP; D011188:Potassium; D009638:Norepinephrine