Hybrid selection of messenger ribonucleoprotein for serum albumin: analysis of specific message-bound proteins.
The messenger RNA for chicken serum albumin together with its specific binding proteins was purified by hybrid selection using filter-bound cloned albumin cDNA. Under conditions where hybridization of the protein-mRNA complex was specific for the immobilized cDNA sequence, no dissociation of the complex into its protein and RNA components was apparent. Electrophoretic analysis of albumin mRNA-specific binding proteins showed three major bands. Two of these were prominent in total poly(A) messenger ribonucleoprotein. The third band was of much greater relative intensity in the albumin mRNA-specific proteins than in total poly(A) messenger ribonucleoprotein. The results suggest that the proteins bound to albumin mRNA represent only a subset of the total population of poly(A) mRNA-associated protein.
Johnson
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Ilan
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D000818:Animals; D002474:Cell-Free System; D002645:Chickens; D002846:Chromatography, Affinity; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis; D008297:Male; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification*; D012261:Ribonucleoproteins / Q000302:isolation & purification*; D012709:Serum Albumin / Q000235:genetics*
D012333:RNA, Messenger; D012261:Ribonucleoproteins; D012709:Serum Albumin; D011061:Poly A; D004247:DNA