title: Modulation of glycogen synthase kinase activity of skeletal and smooth muscle casein kinase I by spermine.
abstract: Casein kinase I (CK-I) from skeletal muscle was stimulated 2-3 fold by 0.25-1 mM spermine. The polyamine also stimulated the phosphorylation of glycogen synthase by another casein kinase purified from aortic smooth muscle [DiSalvo et al. (1986) Biochem. Biophys. Res. Commun. 136, 789-796]. Phosphopeptide maps and phosphoamino acid analysis of [32P]glycogen synthase revealed that smooth muscle casein kinase phosphorylated glycogen synthase in the same sites that undergo phosphorylation by CK-I. The stimulatory effect of spermine on glycogen synthase kinase activity of CK-I was accompanied by increased phosphorylation of all peptide sites of glycogen synthase. Increased phosphorylation was observed in both seryl and threonyl residues. Higher concentrations (4 mM) of spermine inhibited CK-I activity by about 50%. These results indicate that aortic smooth muscle casein kinase is a CK-I enzyme and that skeletal and smooth muscle CK-I can be modulated by spermine.
authors.affiliation: Department of Biochemistry, Medical College of Ohio, Toledo 43699.
authors.forename: M G
authors.initials: MG
authors.lastname: Hegazy
authors.forename: K K
authors.initials: KK
authors.lastname: Schlender
authors.forename: E M
authors.initials: EM
authors.lastname: Reimann
authors.forename: J
authors.initials: J
authors.lastname: DiSalvo
mesh_terms: D000818:Animals; D001011:Aorta / Q000201:enzymology; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D047388:Casein Kinases; D003488:Cyanogen Bromide; D000242:Cyclic AMP / Q000494:pharmacology; D006006:Glycogen Synthase / Q000032:analysis / Q000378:metabolism*; D038341:Glycogen Synthase Kinases; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D010748:Phosphopeptides / Q000032:analysis; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis / Q000378:metabolism; D010769:Phosphothreonine / Q000032:analysis / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D013096:Spermine / Q000494:pharmacology*; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D010748:Phosphopeptides; D010769:Phosphothreonine; D010768:Phosphoserine; D013096:Spermine; D000242:Cyclic AMP; D006006:Glycogen Synthase; D011494:Protein Kinases; D038341:Glycogen Synthase Kinases; D047388:Casein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D014357:Trypsin; D003488:Cyanogen Bromide