Macrophage oxidative burst (OB) and related cytotoxicity--II. Differential sensitivity of erythrocytes from various animals to OB dependent lysis.
Red blood cells (RBCs) from various animals, when exposed to oxidative burst (OB)-stimulated mouse macrophages, showed differences in sensitivity to OB dependent lysis. The increasing order of sensitivity was: mouse, hamster, rabbit, guinea pig, sheep and human RBCs. The degree of OB dependent hemolysis did not correlate with either the capacity of the various cells to degrade H2O2 or their osmotic fragility. The relative sensitivity of the various RBCs to OB products generated by macrophages concurred with their sensitivity to H2O2 generated by an enzymatic system. The differential sensitivity may be correlated with the sphingomyelin content of the cells.
Keisari
Y
Y
Geva
I
I
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000201:enzymology / Q000502:physiology*; D005949:Glucose Oxidase / Q000378:metabolism; D006168:Guinea Pigs; D006461:Hemolysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009996:Osmotic Fragility; D010084:Oxidation-Reduction; D011817:Rabbits; D012756:Sheep
D001786:Blood Glucose; D002860:Chromium Radioisotopes; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase