Processed enzymatically active protease (p15gag) of avian retrovirus obtained in an E. coli system expressing a recombinant precursor (Pr25lac-delta gag).
Processing proteases of avian and mammalian retroviruses cut the polyprotein precursors encoded by the retroviral genes into mature functional proteins. Retroviral processing proteases are still a rather poorly characterized group as to their relation to other proteases, specificity, and mechanism of enzymatic action. In avian retroviruses the generation of the processing protease itself comprises a processing cleavage event - the protease p15gag is cut off the carboxy-terminus of a gag polyprotein precursor, Pr76gag. We report here that direct and efficient production of the avian retrovirus processing protease p15gag (required for structure-function studies and rational design of inhibitors) was obtained in an E. coli system, where massive expression of a size-reduced, recombinant precursor (Pr25lac-delta gag) was accompanied by its structurally accurate processing.
Sedlácek
J
J
Czechoslovak Academy of Sciences, Institute of Molecular Genetics, Prague.
Strop
P
P
Kaprálek
F
F
Pecenka
V
V
Kostka
V
V
Trávnícek
M
M
Ríman
J
J
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics; D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D015683:Gene Products, gag; D005796:Genes; D005814:Genes, Viral; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D012191:Retroviridae Proteins / Q000235:genetics*
D000956:Antigens, Viral; D015683:Gene Products, gag; D011994:Recombinant Proteins; D012191:Retroviridae Proteins