Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice.
Microfluorometric imaging was used to study the correlation of intracellular calcium concentration with voltage-dependent electrical activity in guinea pig cerebellar Purkinje cells. The spatiotemporal dynamics of intracellular calcium concentration are demonstrated during spontaneous and evoked activity. The results are in agreement with hypotheses of dendritic segregation of calcium conductances suggested by electrophysiological experiments. These in vitro slice fluorescence imaging methods are applicable to a wide range of problems in central nervous system biochemical and electrophysiological functions.
Tank
D W
DW
Molecular Biophysics Research Department, AT&T Bell Laboratories, Murray Hill, NJ 07974.
Sugimori
M
M
Connor
J A
JA
Llinás
R R
RR
D000818:Animals; D001572:Benzofurans; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology*; D003712:Dendrites / Q000502:physiology*; D016257:Fura-2; D006168:Guinea Pigs; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008564:Membrane Potentials; D009498:Neurotoxins / Q000494:pharmacology; D010507:Periodicity; D011689:Purkinje Cells / Q000502:physiology*; D013111:Spider Venoms / Q000494:pharmacology
D001572:Benzofurans; D015220:Calcium Channels; D009498:Neurotoxins; D013111:Spider Venoms; D002118:Calcium; D016257:Fura-2