Identification of Highlands J virus from a Florida horse.
A virus, strain 64A-1519, isolated from the brain of a horse dying of encephalitis in Florida in 1964, was identified as western equine encephalomyelitis (WEE) virus. Recently, we used polyclonal and monoclonal immune reagents to identify this isolate by comparing it to 2 strains of WEE virus and to Highlands J (HJ) virus in hemagglutination-inhibition, immunofluorescent antibody, and plaque-reduction neutralization tests. These tests demonstrate that strain 64A-1519 is a strain of HJ virus distinct from WEE virus.
Karabatsos
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Division of Vector-Borne Viral Diseases, Center for Infectious Diseases, U.S. Department of Health and Human Services, Fort Collins, Colorado 80522.
Lewis
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AL
Calisher
C H
CH
Hunt
A R
AR
Roehrig
J T
JT
D000524:Alphavirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002478:Cells, Cultured; D002642:Chick Embryo; D003429:Cross Reactions; D004660:Encephalitis / Q000382:microbiology / Q000662:veterinary*; D004667:Encephalitis Virus, Western Equine / Q000145:classification / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005431:Florida; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D009500:Neutralization Tests; D014036:Togaviridae Infections / Q000382:microbiology / Q000662:veterinary*
D000911:Antibodies, Monoclonal