title: Yeast KEX2 genes encodes an endopeptidase homologous to subtilisin-like serine proteases.
abstract: Yeast Saccharomyces cerevisiae KEX2 gene previously isolated, was characterized as the gene encoding a calcium-dependent endopeptidase required for processing of precursors of alpha-factor and killer toxin. In this study, we report the amino acid sequence of the KEX2 gene product deduced from nucleotide sequencing. Our results indicate that the KEX2 gene contains a 2,442-bp open reading frame encoding a polypeptide of 814 amino acids. The deduced amino acid sequence contains a region extensively homologous to the members of subtilisin-like serine protease family near the N-terminus. A putative membrane-spanning domain near the C-terminus was also detected. These facts indicate that the KEX2-encoded protein may function as a membrane-bound, subtilisin-like serine protease.
authors.affiliation: Department of Biochemistry, Miyazaki Medical College, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mizuno
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Ohshima
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Matsuo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D010450:Endopeptidases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005800:Genes, Fungal; D000072235:Mating Factor; D008969:Molecular Sequence Data; D009154:Mutation; D009710:Nucleotide Mapping; D010455:Peptides / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics*; D013045:Species Specificity; D013381:Subtilisins / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D010455:Peptides; D000072235:Mating Factor; D004262:DNA Restriction Enzymes; D010450:Endopeptidases; D012697:Serine Endopeptidases; D013381:Subtilisins