title: [Aminoglycoside 3'-phosphotransferase gene: its cloning, characteristics and use as a molecular probe].
abstract: Aminoglycoside resistance genes were cloned from transconjugates of aminoglycoside resistant clinical strains of gramnegative bacteria. The resistance determinant cloned from the strains of E. coli transferred kanamycin, monomycin and neomycin resistance to laboratory strains. It was shown that the cloned resistance gene encoded the type I 3'-aminoglycoside phosphotransferase. The results of spot and blot hybridization of the gramnegative bacteria clinical strains with the Bam HI-Pst I fragment of the cloned resistance determinant were indicative of wide-spread distribution of the APH 3' (I) and closely related genes in clinical microbial populations.
authors.forename: S B
authors.initials: SB
authors.lastname: Vakulenko
authors.forename: E G
authors.initials: EG
authors.lastname: Entina
authors.forename: I P
authors.initials: IP
authors.lastname: Fomina
authors.forename: S M
authors.initials: SM
authors.lastname: Navashin
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D066298:In Vitro Techniques; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D011815:R Factors
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D015342:DNA Probes; D004269:DNA, Bacterial; D010770:Phosphotransferases; D019868:Kanamycin Kinase
vernacular_title: Gen 3'-aminoglikozidfosfotransferazy: klonirovanie, kharakteristika, ispol'zovanie v kachestve molekuliarnogo zonda.