title: Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma.
abstract: Using a new Ly-6C-specific antibody (Monts-1) we show that this class of antigens are differentially expressed on monocytes/macrophages and endothelial cells. Recently elicited peritoneal exudate Mac-1+ mononuclear cells, as well as Mac-1+ mononuclear cells in the bone marrow and in the peripheral blood, express high levels of Ly-6C. Ly-6C+ mononuclear Mac-1+ cells are absent in normal uninflamed skin, but are present in high numbers in skin lesions 3 days after the s.c. injection of lipopolysaccharide, concanavalin A or complete Freund's adjuvant. In addition, large Ly-6C+ mononuclear cells are predominant in chronic granulomas induced by complete Freund's adjuvant. Resident macrophages in a variety of tissues express low levels or in many cases do not express Ly-6C. Two out of three monocyte-like cell lines are Ly-6C+, whereas macrophage-like cell lines are negative. Ly-6C+ monocytes/macrophages lose the Ly-6C antigen within 24 h after in vitro culture. Ly-6C- cultured monocytes and Ly-6C- monocyte-like cell lines, but not fully differentiated macrophages and macrophage-like cell lines, can be induced to express the Ly-6C antigen by interferon-gamma. A population of small vessel endothelial cells in diverse tissues also express high levels of Ly-6C. The present findings suggest that the Ly-6C antigen family, shown by others to be involved in T cell activation, may have more general importance in immune responses and cellular differentiation than previously appreciated.
authors.affiliation: Department of Pathology, Stanford University, CA 94305.
authors.forename: M A
authors.initials: MA
authors.lastname: Jutila
authors.forename: F G
authors.initials: FG
authors.lastname: Kroese
authors.forename: K L
authors.initials: KL
authors.lastname: Jutila
authors.forename: A M
authors.initials: AM
authors.lastname: Stall
authors.forename: S
authors.initials: S
authors.lastname: Fiering
authors.forename: L A
authors.initials: LA
authors.lastname: Herzenberg
authors.forename: E L
authors.initials: EL
authors.lastname: Berg
authors.forename: E C
authors.initials: EC
authors.lastname: Butcher
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D004727:Endothelium / Q000276:immunology*; D005434:Flow Cytometry; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000145:classification / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D009000:Monocytes / Q000145:classification / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
chemical_list: D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D000954:Antigens, Surface; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D007371:Interferon-gamma