Comparison of immunohistochemical staining of a mitochondrial protein, lipoamide dehydrogenase, with Fab'-peroxidase conjugates prepared by maleimide or periodate.
IgG-maleimide peroxidase, Fab'-maleimide peroxidase, polymer and monomer types of Fab'-periodate peroxidase were prepared from an antibody against rat lipoamide dehydrogenase, a component of the pyruvate dehydrogenase complex which is located in mitochondria. They were examined for immunohistochemical staining of the rat kidney. Fab'-maleimide peroxidase was the best for staining mitochondrial protein. IgG-maleimide peroxidase and the monomer type of Fab'-periodate peroxidase had the same intensity of staining. The polymer type of Fab'-periodate peroxidase could not stain the lipoamide dehydrogenase.
Matuda
S
S
Department of Biochemistry, School of Medicine, Kagoshima University, Japan.
Yagi
Y
Y
Saheki
T
T
Hashida
S
S
Ishikawa
E
E
D000818:Animals; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007668:Kidney / Q000201:enzymology*; D008301:Maleimides; D010504:Periodic Acid; D010544:Peroxidases; D051381:Rats
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D008301:Maleimides; D010504:Periodic Acid; C043592:maleimide; D010544:Peroxidases; D008058:Dihydrolipoamide Dehydrogenase