The regulation of herpes simplex virus-specific CTL induction by suppressor cells.
The incubation of herpes simplex virus (HSV) immune murine splenocytes with HSV antigens induced suppressor cells which inhibited HSV-specific cytotoxic T lymphocyte (CTL) induction. The cell mediating the suppression was identified as a Thy 1+ Lyt 2+ I-J+ cell. The induction of this suppressor cell required the participation of at least three leukocyte populations. That is, depleting the cultures of either Lyt 1+ or Lyt 2+ splenocytes resulted in a failure to induce suppressor cell activity. Likewise the removal of macrophage-like antigen-presenting cells (APC), in particular I-A- I-J+ APC, abolished suppressor-cell induction. Though the Lyt 2+ I-J+ cells had to be provided by HSV-immune mice, both the APC and the Lyt 1+ cells could be provided by HSV-naive mice. Though the induction of the suppressor cell was virus specific, its action was nonspecific as evidenced by the suppression of influenza-specific CTL induction. The implication of our results for the understanding and manipulation of herpesvirus disease is briefly discussed.
Horohov
D W
DW
Center for Drugs and Biologics, Division of Virology, Food and Drug Administration, Bethesda, MD 20892.
Moore
R N
RN
Rouse
B T
BT
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D003520:Cyclophosphamide / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D018139:Simplexvirus / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000956:Antigens, Viral; D003520:Cyclophosphamide