Tumour-associated antigens in primary mouse fibroblasts induced by transformation with bovine papillomavirus type 1.
Bovine papillomavirus type 1 (BPV 1) DNA was used to transform primary fibroblasts of C57BL/6J mice. Transformation frequency in these cells was much lower than in C127 cells and not associated with the appearance of morphologically distinct foci. However, continuous lines of transformed C57BL/6J cells were developed by serial subculturing of transfected cells. These transformed cell lines showed phenotypic properties associated with transformation including abnormal karyotypes. They contained variable amounts of viral DNA, but the copy number was in the same range as in six C127 transformants tested for comparison. In two cell lines monomeric viral DNA in an episomal form was detected. Slowly migrating viral sequences in these and in the third line were probably episomal concatamers, but the possibility of integration could not be excluded. There was some variation in immunogenicity, but all cell lines induced a cell-mediated immune response in syngeneic mice detected by the chromium release assay. In addition to BPV 1-transformed cell lines, the effector cells also reacted against an unidentified antigen shared by 2 cell lines transformed by SV40 and UV irradiation, respectively.
Mäntyjärvi
R A
RA
Department of Clinical Microbiology, University of Kuopio, Finland.
Sarkkinen
H
H
Karajalainen
H
H
Parkkinen
S
S
Ryhänen
A
A
Syrjänen
K
K
Jägerroos
H
H
Nurmi
T
T
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D015139:Blotting, Southern; D052688:Bovine papillomavirus 1 / Q000276:immunology*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000276:immunology*; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis*; D005347:Fibroblasts / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008810:Mice, Inbred C57BL; D027383:Papillomaviridae / Q000276:immunology*
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral