Studies in metachromatic leukodystrophy: XV. Purification of normal and mutant arylsulfatase A from human liver.
In this report we describe a method to purify both normal and abnormal (inactive) arylsulfatase A. The abnormal enzyme protein was isolated both from cases of late infantile and early juvenile forms of metachromatic leukodystrophy. Conventional protein isolation methods reported earlier were followed by size exclusion high-performance liquid chromatography in the final purification stages. Both the mutant enzyme and the normal enzyme had the same HPLC elution behavior. They thus appeared to self-associate in a similar pH-dependent fashion. Both could be followed by their reaction to a rabbit antibody to normal human arylsulfatase A. The amount of homogenous protein obtained from about 500 grams of liver was 300-400 micrograms.
James
G T
GT
Thach
A B
AB
Klassen
L
L
Austin
J H
JH
D002553:Cerebroside-Sulfatase / Q000302:isolation & purification*; D002648:Child; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007966:Leukodystrophy, Metachromatic / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D013429:Sulfatases / Q000302:isolation & purification*
D013429:Sulfatases; D002553:Cerebroside-Sulfatase