Molecular analysis of chicken immune response genes.
We have recently isolated immune response genes of the major histocompatibility B complex of the chicken (the B-L beta genes) by cross-hybridization in low stringency with an HLA class II beta chain probe. After reviewing the main results obtained, we present a detailed analysis of the region flanking the first gene characterized, B-L beta III. By Southern blot analysis with exon-specific probes, we demonstrate the presence of another related B-L beta gene 10 kb on the 3' side of B-L beta III, the B-L beta V gene. Moreover, retrospective analysis of the phage clones initially isolated with the HLA-DQ beta probe, using a chicken class I probe that we isolated by chromosome walking from the B-L beta genes, indicates that the B-L beta III gene is closely linked on its 5' side to a class I gene, B-FVI.
Béhar
G
G
Institut d'Embryologie du CNRS et du Collège de France, Nogent-sur-Marne.
Bourlet
Y
Y
Fréchin
N
N
Guillemot
F
F
Zoorob
R
R
Auffray
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D005802:Genes, MHC Class II; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping
D015342:DNA Probes; D004262:DNA Restriction Enzymes