Cell numbers and ploidy classes in the normal bladder urothelium in hairless mice. Based on an improved method for dispersion and preparation of cells for flow cytometry.
A simple method for obtaining high-yield single cell suspensions from mouse urothelium is reported. The yield of cells per bladder is about 5 X 10(5), or more than twice that of previously published methods. The method gives a satisfactory coefficient of variation and regularly low levels of debris when used for flow cytometry.
Reitan
J B
JB
D000818:Animals; D002452:Cell Count; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008807:Mice, Inbred BALB C; D011003:Ploidies; D001743:Urinary Bladder / Q000166:cytology*