DNA hybridization for detection of human cytomegalovirus in bronchoalveolar lavage and pharynx biopsy.
A simplified hybridization procedure was used for detection of cytomegalovirus (CMV) in human specimens. The probe was a 32P-labelled cloned DNA fragment of CMV strain AD169. This probe did not hybridize to DNA from uninfected cells or other herpesviruses (herpes simplex virus, Epstein-Barr virus). Specific hybridization was obtained with unselected bronchoalveolar lavage specimens, but the sensitivity of the test (33%) was lower than that of culture (80%) and immunofluorescence (60%) assays which are routinely performed in our laboratory. The detection procedure was also carried out with pharynx biopsy specimens which had been kept frozen at -70 degrees C. CMV DNA was detected in 14% of tumour specimens and only in 1.7% of control specimens (p less than 0.05). The indications of DNA hybridization for CMV diagnosis are discussed.
Agut
H
H
Laboratoire de Virologie, Hôpital Trousseau, Paris.
Deny
P
P
Rousseau
E
E
Garbarg-Chenon
A
A
Beliveau
C
C
Chabolle
F
F
Chouard
H
H
Bricout
F
F
Nicolas
J C
JC
D001992:Bronchoalveolar Lavage Fluid; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D015342:DNA Probes; D005455:Fluorescent Antibody Technique; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010614:Pharynx / Q000382:microbiology
D015342:DNA Probes