Transamination of LTE4 by cysteine conjugate aminotransferase.
Leukotriene E4 (LTE4) was transaminated to 5(S)-hydroxy-6(R)-S-(2-keto-3-thiopropionyl)-7,9-trans-11,14-cis- eicosatetraenoic acid (tentatively designated as LTG4) by cysteine conjugate aminotransferase I purified from rat liver supernatant in the presence of alpha-ketoglutaric acid or alpha-keto-gamma-methiolbutyric acid. The transamination activity was present in the kidney as well as in the liver, but not in the lung or leukocytes.
Tomisawa
H
H
Department of Drug Metabolism, Nippon Roche Research Center, Kanagawa Pref., Japan.
Takanohashi
Y
Y
Ichihara
S
S
Fukazawa
H
H
Tateishi
M
M
D000818:Animals; D007656:Ketoglutaric Acids / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D017999:Leukotriene E4; D015289:Leukotrienes / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D013058:Mass Spectrometry; D008715:Methionine / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000031:analogs & derivatives* / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism*
D007656:Ketoglutaric Acids; D015289:Leukotrienes; D013189:SRS-A; C057290:5-hydroxy-6-(2-keto-3-thiopropionyl)-7,9,11,14-eicosatetraenoic acid; C014632:2-keto-4-methylthiobutyric acid; D017999:Leukotriene E4; D008715:Methionine; D000637:Transaminases; C019968:cysteine aminotransferase