title: Regulation of lipoprotein lipase activity in the sand rat: effect of nutritional state and cAMP modulation.
abstract: The sand rat (Psammomys obesus) is a desert rodent in which obesity and diabetes mellitus appeared only subsequent to feeding laboratory animal chow. To study the role of lipoprotein lipase in the development and maintenance of obesity in the sand rat, enzyme activity in various organs and in plasma of sand rats or albino rats was determined following a 20-hour fast, or 16 hours after injection of cholera toxin. Despite comparable change in body weight, an altered pattern of enzyme distribution and regulation was observed in the sand rat. Neither fasting nor cholera toxin had an effect on heart and daiphragm muscle lipoprotein lipase activity of the sand rat, but caused a 1.5- to 2-fold increase in the treated albino rats. By using an isolated perfused heart system, we were able to measure enzyme activity present in the heparin-releasable fraction that represents the functional pool of the enzyme. In both species, the heparin-releasable fraction of the heart increased twofold following fasting, though initial values were lower in sand rat. In both species, fasting and cholera toxin administration resulted in an increase in plasma and liver lipoprotein lipase activity. Adipose tissue lipoprotein lipase activity of sand rat, unlike the albino rats, was similar in the various fat regions and was not lowered by food deprivation or cholera toxin administration. After both treatments, sand rat plasma insulin levels exceeded fivefold those of albino rats. Adipose tissue lipoprotein lipase activity of fed and fasted normal and diabetic sand rats correlated negatively with plasma insulin and glucose levels.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine B, Hadassah University Hospital, Jerusalem, Israel.
authors.forename: T
authors.initials: T
authors.lastname: Chajek-Shaul
authors.forename: E
authors.initials: E
authors.lastname: Ziv
authors.forename: G
authors.initials: G
authors.lastname: Friedman
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: J
authors.initials: J
authors.lastname: Adler
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D003411:Arvicolinae; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D005215:Fasting; D007328:Insulin / Q000097:blood; D008071:Lipoprotein Lipase / Q000032:analysis*; D008297:Male; D009765:Obesity / Q000201:enzymology*; D051381:Rats; D013045:Species Specificity
chemical_list: D007328:Insulin; D002772:Cholera Toxin; D000242:Cyclic AMP; D008071:Lipoprotein Lipase