Acid hydrolase activities in B cell chronic lymphocytic leukaemia lymphocytes: correlation of cytochemical reactions with immunological phenotype.
The cytochemical reactions of 5 acid hydrolases, alpha-naphthyl acetate esterase (ANAE), acid phosphatase (AP), beta-glucuronidase, beta-glucosaminidase and dipeptidylaminopeptidase IV (DAP IV) were investigated in lymphocytes from 30 patients with B cell chronic lymphocytic leukaemia (B-CLL). Based on ANAE and AP reactivities, 4 cytochemically distinctive subgroups were identified: Group 1: AP and ANAE less than 50% positive lymphocytes (5 cases); Group 2: AP greater than 50%, ANAE less than 50% positive lymphocytes (11 cases); Group 3: AP less than 50%, ANAE greater than 50% positive lymphocytes (7 cases); Group 4: AP and ANAE greater than 50% positive lymphocytes (7 cases). beta-Glucuronidase displayed similar patterns of reactivity to AP. beta-Glucosaminidase activity was observed in the majority of lymphocytes in most patients, whereas DAP IV activity was present in less than 20% of lymphoid cells. The study failed to establish any relationship between cytochemical grouping and patients' clinical status, peripheral lymphocyte counts, E or mouse rosette values, light or heavy chain cellular immunoglobulin (Ig) class. Attempts to correlate acid hydrolase and Ig heavy chain isotype expression, putative markers of B cell maturation, were unsuccessful and indicate that within the narrow spectrum of B cell differentiation seen in B-CLL these characteristics are unrelated.
Crockard
A D
AD
Macfarlane
E
E
Markey
G M
GM
McConnell
R E
RE
Alexander
H D
HD
Agnew
A N
AN
Morris
T C
TC
Bridges
J M
JM
D000135:Acid Phosphatase / Q000097:blood; D000368:Aged; D001402:B-Lymphocytes / Q000201:enzymology*; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000097:blood; D005260:Female; D005966:Glucuronidase / Q000097:blood; D006596:Hexosaminidases / Q000097:blood; D006801:Humans; D006867:Hydrolases / Q000097:blood*; D007136:Immunoglobulins / Q000032:analysis; D007945:Leukemia, Lymphoid / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009283:Naphthol AS D Esterase / Q000097:blood; D010641:Phenotype; D012397:Rosette Formation
D007136:Immunoglobulins; D006867:Hydrolases; D009283:Naphthol AS D Esterase; D000135:Acid Phosphatase; D006596:Hexosaminidases; D005966:Glucuronidase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4