Effect of natural human interferon-beta on the replication of human cytomegalovirus.
The antiviral effect of natural human interferon-beta (HuIFN-beta) against human cytomegalovirus (CMV) was evaluated in human embryonic lung fibroblasts (HEL). Natural HuIFN-beta, like other HuIFNs, inhibited the replication of CMV. Pretreatment of the cells with natural HuIFN-beta inhibited the appearance of immediate-early antigen (IEA) or pre-early nuclear antigen (PENA) as well as the production of infectious CMV. After a single treatment with natural HuIFN-beta, intracellular 2', 5'-oligoadenylate (2-5A) synthetase activity was induced and maintained at a high level for several days. The anti-CMV effect of natural HuIFN-beta correlated with the intracellular 2-5A synthetase activity.
Nakamura
K
K
Department of Microbiology, Miyazaki Medical College, Japan.
Eizuru
Y
Y
Minamishima
Y
Y
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis / Q000378:metabolism; D000956:Antigens, Viral / Q000096:biosynthesis; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000502:physiology*; D004790:Enzyme Induction; D006801:Humans; D017874:Immediate-Early Proteins; D007370:Interferon Type I / Q000494:pharmacology*; D011863:Radioimmunoassay; D013997:Time Factors; D014779:Virus Replication
D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D007370:Interferon Type I; C044582:immediate-early proteins, cytomegalovirus; D015088:2',5'-Oligoadenylate Synthetase