Transcriptional regulation of the thyroperoxydase gene by thyrotropin and forskolin.
The expression of the gene coding for thyroperoxydase, the main enzyme involved in the synthesis of the thyroid hormones, is controlled by thyroid stimulating hormone. In vitro transcription assays performed on nuclei isolated from dog thyroid cells in primary culture showed that this control is at the transcription level, takes place rapidly (1 h) and is cyclic AMP-dependent as it is mimicked by forskolin. Insulin does not seem to be an important modulator of the thyroperoxydase gene expression.
Gerard
C M
CM
Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium.
Lefort
A
A
Libert
F
F
Christophe
D
D
Dumont
J E
JE
Vassart
G
G
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D004285:Dogs; D007328:Insulin / Q000494:pharmacology; D007453:Iodide Peroxidase / Q000235:genetics*; D013961:Thyroid Gland / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D007328:Insulin; D005576:Colforsin; D013972:Thyrotropin; D000242:Cyclic AMP; D007453:Iodide Peroxidase