Monoclonal antibodies directed at human T lymphocyte activation antigens cross react with concanavalin A-stimulated canine and baboon peripheral blood lymphocytes.
A panel of 10 monoclonal antibodies directed at activation antigens on human T lymphocytes were tested for cross reactivity with canine and baboon resting and ConA stimulated peripheral blood lymphocytes. Monoclonal antibodies anti-OKT19, anti-OKT-21, and anti-OKT22 labeled a high percentage of both resting and stimulated canine and baboon cells. Anti-OKT24 labeled activated but not resting baboon lymphocytes and did not label canine lymphocytes. Anti-HLA-DR labeled a small percentage of resting baboon lymphocytes (presumably B cells) and a high percentage of activated baboon and resting and activated canine lymphocytes. Anti-OKT14, anti-OKT20, and anti-OKT23 did not label canine or baboon lymphocytes. Anti-OKT9 did not label baboon lymphocytes, but labeled a low percentage of lymphocytes in one dog. Anti-TAC labeled activated but not resting canine and baboon cells.
Greenlee
P G
PG
Department of Pathology, Cornell University Medical College, New York, New York 10021.
Calvano
S E
SE
Quimby
F W
FW
Hurvitz
A I
AI
Antonacci
A C
AC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis*; D003208:Concanavalin A / Q000494:pharmacology; D003429:Cross Reactions; D004285:Dogs; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D010215:Papio; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D003208:Concanavalin A