Characterization of L1210 S cells with low sensitivity to mouse interferon-gamma.
A mouse leukemic cell line L1210 Sg with a low sensitivity to interferon-gamma (IFN-gamma) was described. On the nature of the antiviral action and binding of IFN, L1210 Sg cells were compared with L1210 m cell line which is sensitive to IFN-gamma. For a half reduction of the vesicular stomatitis virus-RNA synthesis, L1210 Sg cells required 500-fold more IFN-gamma than L1210 m cells did. However, both cell lines were induced to the antiviral state to the same extent with IFN-alpha or -beta. L1210 Sg and L1210 m cells were sensitive to the anti-proliferative action of IFN-alpha and -beta, but insensitive to IFN-gamma. (2'-5')Oligoadenylate synthetase was induced in these cell lines by IFN-beta, but not by IFN-gamma, which suggests that the induction of this synthetase may not be responsible for the antiviral action of IFN-gamma. No substantial difference between L1210 Sg and L1210 m cells was found in IFN receptors for IFN-gamma and IFN-beta either in number per cell or in their affinity to corresponding IFN type. However, differences were noted in time course profiles of cell-associated IFN-gamma at 37 C: in L1210 m cells, a rise-and-decay profile of IFN-gamma bound to cells was observed at 37 C, but in L1210 Sg cells, rise and slight decay was observed. On the other hand, a similar rise-and-decay curve of IFN-beta bound to these cells was observed. These results indicated that the low sensitivity of L1210 Sg cells to IFN-gamma may be due to this slight decay of receptor-bound IFN-gamma.
Iwata
A
A
Institute for Virus Research, Kyoto University.
Watanabe
Y
Y
Sakata
T
T
Sokawa
Y
Y
Kawade
Y
Y
D000818:Animals; D001665:Binding Sites; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D007939:Leukemia L1210 / Q000276:immunology / Q000378:metabolism / Q000628:therapy*; D051379:Mice; D011971:Receptors, Immunologic / Q000378:metabolism; D017471:Receptors, Interferon; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007371:Interferon-gamma