[Polymyxin B suppresses the stimulating action of pertussis vaccine on hematopoiesis in mice].
The injection of killed whole-cell pertussis vaccine (PV), prepared from formulated Bordetella pertussis strains 5574 and 305, into mice was shown to produce a stimulating effect on hematopoiesis. This effect was manifested by an increase in the number of endogenic colonies developing in the spleen of mice on days 5 and 9 after their irradiation in a sublethal dose, by the sharp stimulation of the proliferative activity of splenic colony-forming units (CFUs) originating in the bone marrow and by the elevated CFUs level in the peripheral blood. The preliminary incubation of whole-cell PV with polymyxin B, cationic polypeptide selectively reacting with the lipid A moiety of lipopolysaccharide (LPS), led to a sharp decrease in the above-mentioned manifestations of hematopoietic effect of the vaccine, while incubation with cetavlon interacting with the polysaccharide moiety of LPS produced practically no effect on the capacity of the vaccine for stimulating hematopoiesis. The stimulating effect of whole-cell PV on hematopoiesis is supposedly due, to a great extent, to the lipid A moiety of LPS.
Sanin
A V
AV
Lapaeva
I A
IA
D000818:Animals; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D003114:Colony-Forming Units Assay; D003902:Detergents / Q000494:pharmacology; D004347:Drug Interactions; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010567:Pertussis Vaccine / Q000494:pharmacology*; D011112:Polymyxin B / Q000494:pharmacology*; D011113:Polymyxins / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013997:Time Factors; D015164:Vaccines, Inactivated / Q000494:pharmacology
D002593:Cetrimonium Compounds; D003902:Detergents; D010567:Pertussis Vaccine; D011113:Polymyxins; D015164:Vaccines, Inactivated; D011112:Polymyxin B; D000077286:Cetrimonium
Polimiksin B podavliaet stimuliruiushchee deĭstvie kokliusnoĭ vaktsiny na krovetvorenie u mysheĭ.