Maturation-related gene expression of rat astroblasts in vitro studied by two-dimensional polyacrylamide gel electrophoresis.
Maturation of rat astroblasts was induced in vitro by acidic fibroblast growth factor (aFGF), hydrocortisone and dibutyryl cyclic AMP. Cells grown for 20 days were treated for 48 h and labelled with [35S]methionine only during the last 18 h of treatment. Cell proteins solubilized in lithium dodecyl sulfate were submitted to two-dimensional polyacrylamide gel electrophoresis. About 300 radioactive proteins could be analysed visually and compared. All treatments induced visible quantitative and sometimes qualitative changes. A total of 81 proteins had their rate of biosynthesis modified. For some proteins, this rate was changed by only one treatment, while for others it was changed by two or even by the three treatments, mostly in the same way. These results are consistent with the hypothesis that the proteins involved in the maturation process are organized in sets, proteins belonging to one set always being regulated together under a common control. Some sets would be regulated by only one effector while others would be regulated by several effectors.
Loret
C
C
Centre de Neurochimie du CNRS, Strasbourg, France.
Sensenbrenner
M
M
Labourdette
G
G
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D020904:Glia Maturation Factor; D006133:Growth Substances / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D051381:Rats
D020904:Glia Maturation Factor; D006133:Growth Substances; D009419:Nerve Tissue Proteins; D011506:Proteins; D000242:Cyclic AMP; D006854:Hydrocortisone