title: Chlorella viruses isolated in China.
abstract: Plaque-forming viruses of the unicellular, eucaryotic, exsymbiotic, Chlorella-like green algae strain NC64A, which are common in the United States, were also present in fresh water collected in the People's Republic of China. Seven of the Chinese viruses were examined in detail and compared with the Chlorella viruses previously isolated in the United States. Like the American viruses, the Chinese viruses were large polyhedra and sensitive to chloroform. They contained numerous structural proteins and large double-stranded DNA genomes of at least 300 kilobase pairs. Each of the DNAs from the Chinese viruses contained 5-methyldeoxycytosine, which varied from 12.6 to 46.7% of the deoxycytosine, and N6-methyldeoxyadenosine, which varied from 2.2 to 28.3% of the deoxyadenosine. Four of the Chinese virus DNAs hybridized extensively with DNA from the American virus PBCV-1, and three hybridized poorly.
authors.affiliation: Department of Plant Pathology, University of Nebraska, Lincolin 68583-0722.
authors.forename: Y P
authors.initials: YP
authors.lastname: Zhang
authors.forename: D E
authors.initials: DE
authors.lastname: Burbank
authors.forename: J L
authors.initials: JL
authors.lastname: Van Etten
mesh_terms: D001482:Base Composition; D002681:China; D002708:Chlorella; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D014764:Viral Proteins / Q000032:analysis; D018406:Virus Physiological Phenomena; D014780:Viruses / Q000235:genetics / Q000648:ultrastructure; D014871:Water Microbiology
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes