A new radiochemical method for determination of pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase.
A radiochemical method for assaying pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase is described, using [2-14C]pyruvate and [1-14C]acetate, respectively, as radiolabeled precursors. The assay is based on nonenzymatic O-acylation of excess dithioerythritol (DTE) by enzymatically formed acetyl-coenzyme A. [1-14C]Acetyl-DTE is easily extracted from the incubation mixture by organic solvents and separated from the unreacted labeled substrates.
Liedvogel
B
B
D000106:Acetate-CoA Ligase / Q000032:analysis*; D002736:Chloroplasts / Q000201:enzymology; D003066:Coenzyme A Ligases / Q000032:analysis*; D008928:Mitochondria / Q000201:enzymology; D010944:Plants; D011768:Pyruvate Dehydrogenase Complex / Q000032:analysis*
D011768:Pyruvate Dehydrogenase Complex; D003066:Coenzyme A Ligases; D000106:Acetate-CoA Ligase