Purification of cardiac (Na+,K+)-activated adenosine triphosphatase from rat.
A procedure is described for preparation of highly active (Na+,K+)-ATPase from rat heart which has a specific activity of 200-600 mumol Pi/mg/h. The procedure is simple and can be applied to small amounts of heart muscle (approximately 1 g). The ATPase activity was more than 90% sensitive to ouabain (at concentrations up to 1 mM). The ouabain sensitivity is biphasic with about 20% of the ATPase activity being inhibited at approximately 3 X 10(-7) M ouabain.
Watanabe
T
T
Department of Molecular Physiology, National Cardiovascular Center Research Institute, Osaka, Japan.
Tawada
Y
Y
Shigekawa
M
M
D000818:Animals; D001921:Brain / Q000201:enzymology; D002793:Cholic Acids; D003902:Detergents; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012508:Sarcolemma / Q000201:enzymology; D012967:Sodium Dodecyl Sulfate; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*
D002793:Cholic Acids; D003902:Detergents; D012967:Sodium Dodecyl Sulfate; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate