Urinary excretion of brush-border antigen revealed by monoclonal antibody: early indicator of toxic nephropathy.
Mouse monoclonal antibodies against brush-border antigens of the proximal tubule of human kidney were produced by the hybridisation technique. The urinary excretion of a brush-border protein with an apparent molecular weight of 50 000 (BB-50) was measured by a sandwich enzyme-linked immunosorbent assay with a mouse IgG1 against BB-50 and a polyclonal rabbit antiserum against human kidney as coating and second antibodies. The urinary excretion of BB-50 was fifty times higher in patients treated with cisplatin than in a matched control group and twice as high in workers occupationally exposed to water-soluble chromium(VI) compounds as in their matched controls. These findings suggest that the urinary excretion of kidney antigens revealed by monoclonal antibodies is a very sensitive and specific test for the assessment of toxic nephropathies.
Mutti
A
A
Lucertini
S
S
Valcavi
P
P
Neri
T M
TM
Fornari
M
M
Alinovi
R
R
Franchini
I
I
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000652:urine*; D002857:Chromium / Q000506:poisoning*; D002945:Cisplatin / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007674:Kidney Diseases / Q000139:chemically induced / Q000175:diagnosis / Q000276:immunology*; D007687:Kidney Tubules, Proximal / Q000276:immunology*; D008297:Male; D008871:Microvilli / Q000276:immunology*; D008875:Middle Aged; D008970:Molecular Weight; D009369:Neoplasms / Q000188:drug therapy / Q000276:immunology; D009784:Occupational Diseases / Q000139:chemically induced* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D002857:Chromium; D002945:Cisplatin