title: Mapping of the major glycoprotein gene of human cytomegalovirus.
abstract: The gene coding for the most abundant glycoprotein (gp58) of human cytomegalovirus (HCMV), strain AD169, was physically mapped on the viral genome. A monospecific rabbit antiserum against gp58 was used to screen a cDNA library that was constructed from poly(A)+ RNA of HCMV-infected cells in the prokaryotic expression vector lambda gt11. A cDNA clone was identified which synthesized part of the glycoprotein. It allowed localization of the coding region within the right terminal sequence of the HindIII-F fragment between map coordinates 0.344 and 0.380 of HCMV virion DNA.
authors.forename: M
authors.initials: M
authors.lastname: Mach
authors.forename: U
authors.initials: U
authors.lastname: Utz
authors.forename: B
authors.initials: B
authors.lastname: Fleckenstein
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D006023:Glycoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D011061:Poly A; D004247:DNA; D004262:DNA Restriction Enzymes