Insertion of d(pCpG)n.d(pCpG)n into the lacZ gene of Escherichia coli inhibits expression of beta-galactosidase in vivo.
Plasmids that contain a d(pCpG)13.d(pCpG)13 insert instead of a lac operator show a 34-fold decrease of beta-galactosidase synthesis. The same sequence causes a 24-fold decrease when inserted between codons 5 and 6 of the lacZ gene. In such constructs with d(pCpG)n.d(pCpG)n inserts, beta-galactosidase activity decreases approximately 1.6-fold per d(pCpG).d(pCpG) unit when n ranges from 5 to 16.
Horbach
E
E
Institut für Genetik der Universität zu Köln, F.R.G.
Müller-Hill
B
B
D001483:Base Sequence; D004251:DNA Transposable Elements; D003854:Deoxyribonucleotides; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005696:Galactosidases / Q000378:metabolism*; D007763:Lac Operon; D010957:Plasmids; D001616:beta-Galactosidase / Q000378:metabolism*
D004251:DNA Transposable Elements; D003854:Deoxyribonucleotides; D005696:Galactosidases; D001616:beta-Galactosidase