Interleukin 2 acts directly on a Tac-positive cloned B cell line established from a patient with adult T cell leukemia.
A Tac-positive B cell line termed K3B was established from a patient with adult T cell leukemia (ATL). This cell line had EBNA antigen and human T cell leukemic virus (HTLV) provirus besides B1 antigen and surface immunoglobulin. A cloned Tac-positive B cell line termed K3B01 was obtained from K3B by the limiting dilution method. The K3B01 cells were shown to absorb IL 2 activity in a tonsillar IL 2 preparation. By using this cloned cell line and a purified recombinant IL 2 preparation, it was shown that the proliferation of K3B01 cells was enhanced by the addition of recombinant IL 2. Moreover, this response was inhibited by anti-Tac antibody. These results demonstrate definitively that IL 2 acts directly on B cells through IL 2 receptors on them.
Arima
N
N
Daitoku
Y
Y
Hidaka
S
S
Tanaka
H
H
Katsuki
T
T
D000328:Adult; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D017977:Deltaretrovirus; D006800:Deltaretrovirus Infections / Q000276:immunology*; D006133:Growth Substances / Q000502:physiology; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology*; D015847:Interleukin-4; D008222:Lymphokines / Q000502:physiology; D010641:Phenotype; D011971:Receptors, Immunologic / Q000502:physiology; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000954:Antigens, Surface; D006133:Growth Substances; D007376:Interleukin-2; D008222:Lymphokines; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D015847:Interleukin-4