Retinoic acid-induced expression of tissue transglutaminase in human promyelocytic leukemia (HL-60) cells.
Addition of retinoic acid to human promyelocytic leukemia cells results in a dramatic increase in cellular transglutaminase activity. This increase is due to the induction of a specific intracellular transglutaminase, tissue transglutaminase. Retinoic acid-induced expression of tissue transglutaminase is potentiated by analogues of cyclic AMP. The induction of the enzyme can be detected within 6 h of the addition of the retinoid to the cell and results in increases of the enzyme of at least 50-fold. The induction of HL-60 transglutaminase is a specific response of the cells to retinoic acid and is not seen with other agents that induce HL-60 differentiation. We believe that the induction of tissue transglutaminase is a useful index of the early events in retinoid-regulated gene expression in both normal and transformed cells.
Davies
P J
PJ
Murtaugh
M P
MP
Moore
W T
WT
Johnson
G S
GS
Lucas
D
D
D000217:Acyltransferases / Q000096:biosynthesis*; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004126:Dimethylformamide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology*; D012177:Retinol-Binding Proteins / Q000494:pharmacology; D013997:Time Factors; D011503:Transglutaminases; D014212:Tretinoin / Q000494:pharmacology*
D007042:Hypoxanthines; D012177:Retinol-Binding Proteins; D019271:Hypoxanthine; D014212:Tretinoin; D003994:Bucladesine; D004126:Dimethylformamide; D000217:Acyltransferases; D011503:Transglutaminases; D004121:Dimethyl Sulfoxide