Genetic regulation of cell-to-cell communication.
Overexpression of the cellular src gene in NIH-3T3 cells causes reduction of cell-to-cell transmission of molecules in the 400-700 dalton range. This down-regulation of gap junctional communication correlates with the activity of the gene product, the protein tyrosine kinase pp60c-src. The down-regulation is enhanced by point mutation of Tyr527 (a site phosphorylated in pp60c-src and which inhibits kinase activity) or by substitution of the viral- for the cellular-src carboxyl terminal coding region. Mutation of Tyr416 (a site phosphorylated upon Tyr527 mutation) suppresses both the down-regulation by Tyr527 mutation and that by gene overexpression. The regulation of communication by src may be important in the control of embryonic development and cellular growth.
Loewenstein
W R
WR
Department of Physiology and Biophysics, University of Miami School of Medicine, Florida 33101.
D002450:Cell Communication; D002455:Cell Division; D002463:Cell Membrane Permeability; D000242:Cyclic AMP / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007365:Intercellular Junctions / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D014158:Transcription, Genetic
D011948:Receptors, Antigen, T-Cell; D000242:Cyclic AMP