title: [The expression of c-myc, c-fms, c-sis oncogenes in the trophoblast of normal pregnancy and trophoblastic disease].
abstract: Recently it has been suggested that proto-oncogene plays a role not only in cellular proliferation, development and differentiation, but also in neoplastic transformation. We now show the expression and its localization of c-myc, c-fms and c-sis proto-oncogenes in human developing chorionic tissue and fresh surgical specimens of mole and choriocarcinoma with the method of Northern blotting and In-situ hybridization. The 2.4kb c-myc transcript has been localized to the cytotrophoblast in early placenta and also localized to the C and S typed trophoblastic cells in mole and choriocarcinoma. The 4.0kb c-fms transcript has been localized to the syncytiotrophoblast, especially the highly differentiated syncytiotrophoblast in the second and third trimesters and S typed trophoblastic cells in mole and choriocarcinoma. Moreover, the 4.0kb c-sis transcript has been localized to the cytotrophoblast in early placenta, but not detected in mole or choriocarcinoma. First, these results suggest that the stage and site specific expression of c-myc, c-fms and c-sis proto-oncogenes are clearly related to the proliferation, development and differentiation of normal trophoblastic cells. Second, the expression of c-myc, c-fms proto-oncogenes may be of particular importance in the tumorigenesis and progression of trophoblastic disease.
authors.affiliation: Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo.
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.forename: S
authors.initials: S
authors.lastname: Niimi
authors.forename: M
authors.initials: M
authors.lastname: Tsuruoka
authors.forename: T
authors.initials: T
authors.lastname: Kijima
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
authors.forename: N
authors.initials: N
authors.lastname: Komuro
authors.forename: Y
authors.initials: Y
authors.lastname: Terashima
mesh_terms: D015152:Blotting, Northern; D002822:Choriocarcinoma / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011247:Pregnancy; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D014328:Trophoblastic Neoplasms / Q000235:genetics*; D014327:Trophoblasts / Q000648:ultrastructure*; D014594:Uterine Neoplasms / Q000235:genetics*