title: Biochemical characterization of the Ca2+ release channel of skeletal and cardiac sarcoplasmic reticulum.
abstract: Rapid mixing-vesicle ion flux and planar lipid bilayer-single channel measurements have shown that a high-conductance, ligand-gated Ca2+ release channel is present in 'heavy', junctional-derived membrane fractions of skeletal and cardiac muscle sarcoplasmic reticulum. Using the release channel-specific probe, ryanodine, a 30S protein complex composed of polypeptides of Mr approximately 400,000 has been isolated from cardiac and skeletal muscle. Reconstitution of the complex into planar lipid bilayers has revealed a Ca2+ conductance with properties characteristic of the native Ca2+ release channel.
authors.affiliation: Department of Biochemistry, University of North Carolina, Chapel Hill 27599.
authors.forename: G
authors.initials: G
authors.lastname: Meissner
authors.forename: E
authors.initials: E
authors.lastname: Rousseau
authors.forename: F A
authors.initials: FA
authors.lastname: Lai
authors.forename: Q Y
authors.initials: QY
authors.lastname: Liu
authors.forename: K A
authors.initials: KA
authors.lastname: Anderson
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D015220:Calcium Channels / Q000032:analysis*; D002147:Calmodulin / Q000494:pharmacology; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000032:analysis*; D009206:Myocardium / Q000032:analysis*; D012433:Ryanodine; D012519:Sarcoplasmic Reticulum / Q000032:analysis*
chemical_list: D000227:Adenine Nucleotides; D015220:Calcium Channels; D002147:Calmodulin; D012433:Ryanodine; D002110:Caffeine; D008274:Magnesium; D002118:Calcium