Metabolism of 2-methylnaphthalene by rat and rainbow trout hepatic microsomes and purified cytochromes P-450.
The metabolism of 14C-2-methylnaphthalene by hepatic microsomes and purified cytochromes P-450 from rat and rainbow trout was studied using HPLC and radioactivity counting. The effects of pretreatment with beta-naphthoflavone and phenobarbital on the formation of three dihydrodiol metabolites and 2-hydroxymethylnaphthalene by microsomes and purified cytochromes P-450 were compared. The metabolism observed with microsomes could not adequately be accounted for by the combined metabolism observed with the purified cytochromes P-450. Differences in the microsome suspension buffers used when preparing microsomes for cytochrome purification as compared to preparing microsomes for direct use appeared to be a significant factor in these differences.
Melancon
M J
MJ
Williams
D E
DE
Buhler
D R
DR
Lech
J J
JJ
D000818:Animals; D002021:Buffers; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000494:pharmacology*; D004851:Epoxide Hydrolases / Q000032:analysis; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009281:Naphthalenes / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D014337:Trout
D002021:Buffers; D009281:Naphthalenes; D003577:Cytochrome P-450 Enzyme System; D004851:Epoxide Hydrolases; C027384:2-methylnaphthalene