title: [Endocytosis of liposomes by Entamoeba histolytica and transport of encapsulated molecules toward the cytoplasm].
abstract: We have examined the interaction of a highly phagocytosing cell: Entamoeba histolytica with liposomes of different lipid compositions, and followed, by a semi-quantitative method, the intracellular fate of the entrapped molecules. Liposomes containing a small molecule, 6-carboxyfluorescein, are first phagocytosed. Then the encapsulated compound migrates from the vacuoles to the cytoplasm. Liposomes containing macromolecular substances, such as fluorescent albumin or ferritin, are also phagocytosed, but the encapsulated molecules remain within the vacuoles. We conclude that the transfer of carboxyfluorescein does not involve a fusion between liposomes and vacuoles, but more likely occurs via diffusion through membranes. The lipid composition of the liposomes does not affect phagocytosis of liposomes. In contrast, oleic acid greatly increases the transfer of carboxyfluorescein from vacuole to cytoplasm.
authors.forename: P
authors.initials: P
authors.lastname: Trudel
authors.forename: C
authors.initials: C
authors.lastname: Gicquaud
mesh_terms: D001692:Biological Transport; D003593:Cytoplasm / Q000378:metabolism; D004705:Endocytosis; D004748:Entamoeba histolytica / Q000502:physiology*; D005452:Fluoresceins / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008081:Liposomes; D019301:Oleic Acid; D009829:Oleic Acids / Q000502:physiology; D013696:Temperature; D014617:Vacuoles / Q000378:metabolism
chemical_list: D005452:Fluoresceins; D008081:Liposomes; D009829:Oleic Acids; D019301:Oleic Acid; C024098:6-carboxyfluorescein
vernacular_title: Endocytose des liposomes par Entamoeba histolytica et transfert des molécules encapsulées vers le cytoplasme.