Presence of pro-vasopressin mRNA, neurophysin and arginine vasopressin in mouse anterior pituitary cells and the AtT-20 corticotrophic tumour cell line.
Pro-vasopressin mRNA, neurophysin and arginine vasopressin (AVP) were assayed in the mouse anterior pituitary gland, in mouse anterior pituitary cells in culture and in the AtT-20 corticotrophic tumour cell line. Northern blot analysis revealed the presence of an approximately 700 base pair pro-vasopressin mRNA in anterior pituitary and AtT-20 cells. Neurophysin, identified by immunoblots, and AVP, identified by high-performance liquid chromatography and cross-reactivity with AVP antiserum, were detected in anterior pituitary cells and AtT-20 cells. Immunocytochemical staining with anti-neurophysin showed that approximately 40-45% of the dissociated anterior pituitary cells in culture and greater than 95% of the AtT-20 cells were stained. Anterior pituitary cells in culture and AtT-20 cells had a basal level of release of AVP in the 0.01-0.1 nM range. These results indicate that anterior pituitary cells and AtT-20 cells have the ability to synthesize and process pro-vasopressin to AVP and neurophysin, endogenously.
Loh
Y P
YP
Laboratory of Neurochemistry and Neuroimmunology, National Institute of Child Health and Human Development.
Castro
M G
MG
Zeng
F J
FJ
Patel-Vaidya
U
U
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism* / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009481:Neurophysins / Q000378:metabolism*; D010121:Oxytocin; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured / Q000378:metabolism* / Q000503:physiopathology; D014667:Vasopressins / Q000235:genetics* / Q000378:metabolism
D009481:Neurophysins; D011498:Protein Precursors; D012333:RNA, Messenger; D014667:Vasopressins; D001127:Arginine Vasopressin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D010121:Oxytocin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone