Measurement of propericiazine in plasma by capillary gas chromatography and selected ion monitoring detection.
A new method, based upon a selective extraction and gas chromatographic/mass spectrometric analysis, was developed to monitor the effect of combined haemoperfusion-haemodialysis treatment in a case of propericiazine poisoning. The method relies on the selected ion monitoring of the acetate derivatives of propericiazine and its internal standard fluphenazine, after their extraction from 1 ml of alkalinized plasma with n-hexane:isopropanol (8:2, v/v), back-extraction into an acidified water phase, realkalinization and extraction with n-hexane: isopropanol, derivatization with acetic anhydride and gas chromatography on a short (12 m) OV-101 fused silica capillary column. The described procedure is specific and provides between-assay variability of 4.8% CV at 5 micrograms 1(-1) plasma concentration. The method enables quantification down to 1 microgram 1(-1) and hence demonstrates sufficient sensitivity to permit pharmacokinetic or drug monitoring studies.
De Leenheer
A P
AP
Jonckheere
J A
JA
Lefevere
M F
MF
De Broe
M E
ME
Verpooten
G A
GA
D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006464:Hemoperfusion; D006801:Humans; D008875:Middle Aged; D010640:Phenothiazines / Q000097:blood* / Q000506:poisoning; D006435:Renal Dialysis; D013406:Suicide, Attempted
D010640:Phenothiazines