title: Do metal ions promote the re-activation of the 2,3-bisphosphoglycerate-independent phosphoglycerate mutases?
abstract: It has been reported [Smith, McWilliams & Hass (1986) Biochem. Biophys. Res. Commun. 136, 336-340] that addition of certain metal ions, notably Co2+ and Mn2+, promoted the refolding of denatured phosphoglycerate mutase from wheat germ. We have re-investigated these experiments and have shown that, when precautions are taken to avoid artefacts in the assay system, the metal ions do not promote any re-activation of the denatured wheat-germ or Aspergillus nidulans enzymes. An alternative explanation is offered for the observations of Smith et al. (1986).
authors.affiliation: Department of Biological Science, University of Stirling, Scotland, U.K.
authors.forename: C M
authors.initials: CM
authors.lastname: Johnson
authors.forename: N C
authors.initials: NC
authors.lastname: Price
mesh_terms: D019794:2,3-Diphosphoglycerate; D001233:Aspergillus nidulans / Q000201:enzymology; D003035:Cobalt / Q000494:pharmacology*; D004163:Diphosphoglyceric Acids / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D007527:Isoenzymes / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology*; D009492:Neurospora crassa / Q000201:enzymology; D010736:Phosphoglycerate Mutase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011489:Protein Denaturation / Q000187:drug effects; D014908:Triticum / Q000201:enzymology
chemical_list: D004163:Diphosphoglyceric Acids; D007527:Isoenzymes; D019794:2,3-Diphosphoglycerate; D003035:Cobalt; D008345:Manganese; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase