Immunoprotection of mice against Trypanosoma cruzi with a lyophilized flagellar fraction of the parasite plus adjuvant.
The immunization with the flagellar (F) fraction from epimastigotes of Trypanosoma cruzi has been shown to protect mice against a challenge of bloodstream trypomastigotes of the parasite, both in terms of mortality and decrease in parasitemia. We have compared the immunoprotective properties of the fresh F fraction with those of a lyophilized F (LF) fraction, alone or together with Bordetella pertussis (Bp) as adjuvant. The best results were obtained with LF + Bp: after challenge with 1 X 10(3) metacyclic trypomastigotes, 100% of the mice immunized with LF + Bp survived, and 60% of them showed no signs of parasitemia. Only the animals in which patent parasitemia was demonstrated presented heart and muscle infiltrates.
Ruiz
A M
AM
Esteva
M
M
Riarte
A
A
Subías
E
E
Segura
E L
EL
D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000818:Animals; D001886:Bordetella pertussis / Q000276:immunology; D002458:Cell Fractionation; D002598:Chagas Cardiomyopathy / Q000276:immunology* / Q000401:mortality; D005407:Flagella / Q000276:immunology; D005612:Freeze Drying; D007110:Immunity, Active; D007114:Immunization / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology / Q000302:isolation & purification
D000276:Adjuvants, Immunologic