Dinitropyrene-resistant Salmonella typhimurium are deficient in an acetyl-CoA acetyltransferase.
Salmonella typhimurium strain TA98NR which is sensitive to 1,8-dinitropyrene mutagenesis possesses acetyl-CoA dependent acetyltransferase activity, while a strain selected for resistance to 1,8-dinitropyrene (TA98/1, 8-DNP6) is deficient in this activity. Acetyltransferase competent strains can acetylate 1,8-diaminopyrene, forming 1-N-acetylamino-8-aminopyrene and 1,8-N,N'-diacetyldiaminopyrene. The coincidence of dinitropyrene resistance and acetyltransferase deficiency implicates acetylation as an important process in the metabolic activation of dinitropyrene to a mutagenic intermediate.
Orr
J C
JC
Bryant
D W
DW
McCalla
D R
DR
Quilliam
M A
MA
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D000123:Acetyltransferases / Q000235:genetics*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004352:Drug Resistance, Microbial; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D011721:Pyrenes / Q000378:metabolism / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
D009153:Mutagens; D011721:Pyrenes; C033310:1,8-dinitropyrene; D000105:Acetyl Coenzyme A; D000123:Acetyltransferases; D000101:Acetyl-CoA C-Acetyltransferase