Effect of Mycobacterium tuberculosis-derived sulfolipid I on human phagocytic cells.
Experiments were performed to determine the effects of Mycobacterium tuberculosis-derived sulfolipid I on phagocytic cells. Sulfolipid I was taken up in significant amounts by human neutrophils and in lesser amounts by monocytes and lymphocytes. Superoxide (O2-) production by neutrophils was significantly increased by sulfolipid I, but the rate of production was slower than that reported previously for other stimuli. The optimal concentration of sulfolipid I for stimulation of O2- production was 27 micrograms/ml, while higher concentrations produced less. At substimulatory levels sulfolipid I caused enhancement of O2- release from neutrophils when it was subsequently stimulated by other agents. Nonadherent monocytes from most normal donors failed to produce O2- when treated with sulfolipid I; however, adherent monocytes pretreated with gamma interferon did produce O2- with sulfolipid I stimulation. Priming for an enhanced oxidative response of activated monocytes was also observed. These sulfolipid I-induced changes in phagocytic cell function may be important in altering the ability of phagocytes to respond effectively to M. tuberculosis and may also cause exaggerated inflammatory responses.
Zhang
L
L
Department of Medicine, University of Illinois College of Medicine, Chicago.
Goren
M B
MB
Holzer
T J
TJ
Andersen
B R
BR
D002470:Cell Survival / Q000187:drug effects; D003311:Cord Factors / Q000494:pharmacology; D006017:Glycolipids / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D009000:Monocytes / Q000187:drug effects / Q000502:physiology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D010586:Phagocytes / Q000187:drug effects* / Q000276:immunology; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D037521:Virulence Factors
D003311:Cord Factors; D006017:Glycolipids; D037521:Virulence Factors; D013481:Superoxides; C009355:sulfolipid I; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate