The effect of testosterone on citrate synthesis and citrate oxidation and a proposed mechanism for regulation of net citrate production in prostate.
Citrate oxidation by rat ventral prostate was reduced by castration and increased by testosterone administration. Similarly, the mitochondrial aconitase activity was decreased by castration; whereas cytosol aconitase was unaffected. The rate of citrate oxidation is extremely low in prostate. Castration also decreased mitochondrial aspartate aminotransferase activity while having no effect on the cytosol isoenzyme. Testosterone markedly stimulated the net production of citrate from aspartate plus glutamate by prostate mitochondria. These studies support the proposal that aspartate is a major source of oxalacetate for citrate production, and that a "glutamate-aspartate-citrate" pathway may be functional in prostate mitochondria. In addition, testosterone can regulate citrate production by a specific effect on mitochondrial aspartate aminotransferase activity. Testosterone also regulates the flux of citrate through the Krebs cycle, but this represents only a small proportion of the citrate accumulated. These conditions would be consistent with the function of prostate epithelium in accumulating and secreting citrate.
Franklin
R B
RB
Kahng
M W
MW
Akuffo
V
V
Costello
L C
LC
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D002951:Citrates / Q000096:biosynthesis / Q000378:metabolism*; D019343:Citric Acid; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008954:Models, Biological; D009919:Orchiectomy; D010084:Oxidation-Reduction; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000502:physiology; D013739:Testosterone / Q000494:pharmacology*
D002951:Citrates; D005971:Glutamates; D019343:Citric Acid; D001224:Aspartic Acid; D018698:Glutamic Acid; D013739:Testosterone; D000105:Acetyl Coenzyme A; D001219:Aspartate Aminotransferases