Two new tools: multi-purpose cloning vectors that carry kanamycin or spectinomycin/streptomycin resistance markers.
We have constructed two new multi-purpose cloning vectors, pJKKmf(-) and pJKSp/Smf(-), that carry resistance to kanamycin (Km) and spectinomycin/streptomycin (Sp/Sm), respectively. These plasmids are based on pGEM3Zf(-) and therefore contain a pUC-vector-derived multiple cloning site for 13 restriction sites flanked by T7 and SP6 RNA polymerase promoters, the alpha-peptide coding region of beta-galactosidase for blue/white colony screening and the bacteriophage f1 origin of replication for production of single-stranded DNA in the presence of a helper phage. We have used these vectors to reclone sequences from a maize genomic library, to produce radiolabeled RNA probes and to make single-stranded DNA.
Kirschman
J A
JA
Agrigenetics Advanced Science Company, Madison, WI 53716.
Cramer
J H
JH
D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007612:Kanamycin / Q000494:pharmacology*; D011815:R Factors; D000198:Spectinomycin / Q000494:pharmacology*; D013307:Streptomycin / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000235:genetics
D007612:Kanamycin; D000198:Spectinomycin; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase; D013307:Streptomycin