Plant glutamine synthetase complements a glnA mutation in Escherichia coli.
A glutamine synthetase gene from alfalfa (Medicago sativa) has been expressed in Escherichia coli after fusion of bacterial transcription and translation signals to a complete alfalfa glutamine synthetase coding sequence. Synthesis of the alfalfa glutamine synthetase enzyme in Escherichia coli was demonstrated by functional genetic complementation of a glutamine synthetase-deficient mutant and by immunoblotting analysis. These results should facilitate protein engineering and structure-function analysis of the plant enzyme.
DasSarma
S
S
Tischer
E
E
Goodman
H M
HM
D001483:Base Sequence; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005816:Genetic Complementation Test; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D000455:Medicago sativa / Q000235:genetics*; D008970:Molecular Weight; D010957:Plasmids; D011401:Promoter Regions, Genetic
D004274:DNA, Recombinant; D005974:Glutamate-Ammonia Ligase