Detection of Coxsackie-B-virus-specific RNA sequences in myocardial biopsy samples from patients with myocarditis and dilated cardiomyopathy.
Full-length virus genomic RNA (7.4 kilobases; kb) was isolated from Coxsackie B2 virus purified from infected monkey kidney cells in culture. DNA complementary to 6.3 kb of virus RNA was prepared by reverse transcription and cloned in a plasmid vector. A 1.6 kb Coxsackie-B-virus-specific DNA clone derived from the conserved 3' region of the virus genome was used as a hybridisation probe to test for the presence of virus nucleic acid sequences in myocardial biopsy samples. Positive hybridisation signals, quantified by densitometry, were obtained with 9 of 17 samples from patients with histological evidence of active or healing myocarditis or dilated cardiomyopathy with inflammatory changes. No Coxsackie-B-virus-specific sequences were detected in 4 samples from patients in whom a viral aetiology was unlikely and the histological diagnosis was negative for myocarditis.
Bowles
N E
NE
Richardson
P J
PJ
Olsen
E G
EG
Archard
L C
LC
D000293:Adolescent; D000328:Adult; D000368:Aged; D002311:Cardiomyopathy, Dilated / Q000382:microbiology*; D002648:Child; D003001:Cloning, Molecular; D003384:Coxsackievirus Infections / Q000382:microbiology*; D004247:DNA; D029822:Enterovirus B, Human / Q000235:genetics*; D005260:Female; D006321:Heart / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009205:Myocarditis / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*
D012367:RNA, Viral; D004247:DNA