title: Different diuresis-dependent excretions of urinary enzymes: N-acetyl-beta-D-glucosaminidase, alanine aminopeptidase, alkaline phosphatase, and gamma-glutamyltransferase.
abstract: We studied how much of the lysosomal enzyme N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30) and of the brush-border enzymes alanine aminopeptidase (EC 3.4.11.2), alkaline phosphatase (EC 3.1.3.1), and gamma-glutamyltransferase (EC 2.3.2.2) was excreted in urine over 8 h after a high intake of fluid (22 mL per kilogram of body weight). The hourly excretion of all four enzymes increased with the increasing urine flow rate. The excretion rate of the brush-border enzymes was more markedly influenced than that of N-acetyl-beta-D-glucosaminidase. By relating the enzyme excretion to urinary creatinine we could reduce the variability of brush-border enzyme output and could completely compensate for the effect of diuresis on the excretion of N-acetyl-beta-D-glucosaminidase.
authors.forename: K
authors.initials: K
authors.lastname: Jung
authors.forename: G
authors.initials: G
authors.lastname: Schulze
authors.forename: C
authors.initials: C
authors.lastname: Reinholdt
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000328:Adult; D000469:Alkaline Phosphatase / Q000652:urine; D000626:Aminopeptidases / Q000652:urine; D018826:CD13 Antigens; D003404:Creatinine / Q000652:urine; D004231:Diuresis; D004798:Enzymes / Q000652:urine*; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008871:Microvilli / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000652:urine
chemical_list: D004798:Enzymes; D003404:Creatinine; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D000118:Acetylglucosaminidase; D000626:Aminopeptidases; D018826:CD13 Antigens