Stimulation of guanylate cyclase by atrial natriuretic factor in isolated human glomeruli.
A 23 amino acid synthetic peptide fragment of atrial natriuretic factor (ANF) stimulated guanylate cyclase activity in isolated human glomeruli in a concentration- and time-dependent manner. ANF activated particulate guanylate cyclase whereas it had no effect on soluble guanylate cyclase. These results demonstrate that the glomerulus is a target structure for ANF in humans. They also suggest that ANF-induced increase in glomerular filtration rate is due to a direct effect of this peptide on the glomerular cells mediated by activation of glomerular guanylate cyclase.
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D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP / Q000378:metabolism; D004789:Enzyme Activation; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D007678:Kidney Glomerulus / Q000187:drug effects / Q000201:enzymology*; D045283:Natriuretic Agents; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins / Q000494:pharmacology*; D013997:Time Factors
C472955:NPPA protein, human; D045283:Natriuretic Agents; D010446:Peptide Fragments; D011506:Proteins; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D006152:Cyclic GMP