title: Structure and functional expression of cloned rat serotonin 5HT-2 receptor.
abstract: A complementary DNA (cDNA) encoding a serotonin receptor with 51% sequence identity to the 5HT-1C subtype was isolated from a rat brain cDNA library by homology screening. Transient expression of the cloned cDNA in mammalian cells was used to establish the pharmacological profile of the encoded receptor polypeptide. Membranes from transfected cells showed high-affinity binding of the serotonin antagonists spiperone, ketanserin and mianserin, low affinity for haloperidol (a dopamine D2 receptor antagonist), 8-OH-DPAT as well as MDL-72222 and no detectable binding of [3H]serotonin. This profile is consonant with the 5HT-2 subtype of serotonin receptors. In agreement with this assignment, serotonin increased the intracellular Ca2+ concentration and activated phosphoinositide hydrolysis in transfected mammalian cells. The agonist also elicited a current flow, blocked by spiperone, in Xenopus oocytes injected with in vitro synthesized RNA containing the cloned nucleotide sequences.
authors.affiliation: Laboratory of Molecular Neuroendocrinology, University of Heidelberg, FRG.
authors.forename: D B
authors.initials: DB
authors.lastname: Pritchett
authors.forename: A W
authors.initials: AW
authors.lastname: Bach
authors.forename: M
authors.initials: M
authors.lastname: Wozny
authors.forename: O
authors.initials: O
authors.lastname: Taleb
authors.forename: R
authors.initials: R
authors.lastname: Dal Toso
authors.forename: J C
authors.initials: JC
authors.lastname: Shih
authors.forename: P H
authors.initials: PH
authors.lastname: Seeburg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006868:Hydrolysis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009865:Oocytes / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D011985:Receptors, Serotonin / Q000235:genetics* / Q000378:metabolism; D013134:Spiperone / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D010716:Phosphatidylinositols; D011985:Receptors, Serotonin; D013134:Spiperone; D004247:DNA; D002118:Calcium