title: Eicosapentaenoic acid metabolism in cynomolgus and rhesus conjunctiva and eyelid.
abstract: We have demonstrated that cynomolgus and rhesus monkey ocular tissues such as conjunctiva and eyelids have the capacity to synthesize cyclo-oxygenase and lipoxygenase products from arachidonic acid (AA). In the present study, we demonstrate that another fatty acid, eicosapentaenoic acid (C20:5), which is found mainly in marine diets, can be converted by cynomolgus and rhesus monkey conjunctiva and eyelids into cyclo-oxygenase (3-series) and lipoxygenase (5-series) products. EPA-lipoxygenase activity (ranging from 6.5% to 10.4%) was greater than EPA-cyclo-oxygenase activity (ranging from 1.5% to 3.5%) in these tissues. Furthermore, BW755, a cyclo-oxygenase and lipoxygenase inhibitor, inhibited the synthesis of EPA-derived cyclo-oxygenase and lipoxygenase product synthesis, indicating the enzymatic nature of EPA metabolism.
authors.affiliation: Department of Pharmacology, Columbia University, New York, New York.
authors.forename: P S
authors.initials: PS
authors.lastname: Kulkarni
authors.forename: P L
authors.initials: PL
authors.lastname: Kaufman
authors.forename: B D
authors.initials: BD
authors.lastname: Srinivasan
mesh_terms: D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D000818:Animals; D002845:Chromatography / Q000379:methods; D003228:Conjunctiva / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D005143:Eyelids / Q000378:metabolism*; D008084:Lipoxygenase / Q000096:biosynthesis; D016859:Lipoxygenase Inhibitors; D008251:Macaca / Q000378:metabolism*; D008252:Macaca fascicularis / Q000378:metabolism*; D008253:Macaca mulatta / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis; D011720:Pyrazoles / Q000494:pharmacology
chemical_list: D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D011720:Pyrazoles; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D015118:Eicosapentaenoic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases