Structure and expression of c-fgr protooncogene mRNA in Epstein-Barr virus converted cell lines.
The c-fgr protooncogene is a member of the c-src family of tyrosine kinases. Expression of c-fgr was studied in a series of Epstein-Barr virus (EBV) negative Burkitt's lymphoma cell lines and their EBV-converted derivatives. Two transcripts, of 2.9 kb and 3.5 kb, were present at dramatically elevated levels following EBV-conversion. The structure of the c-fgr transcripts was studied by the isolation and nucleotide sequence analysis of cDNA clones. This indicated that the c-fgr protein encoded by the mature mRNA would contain 529 amino acids and have a molecular weight of approximately 58,000. The N-terminus of the predicted c-fgr protein has low amino acid homology with the N-termini of other members of this family of proteins, suggesting a cell specific function for the N-terminal domain. Analysis of the c-fgr cDNA clones also revealed the presence of alternative functional polyadenylation signals, although the use of these does not account for the size difference between the two major c-fgr transcripts.
Brickell
P M
PM
Department of Biochemistry, University College and Middlesex School of Medicine, London, UK.
Patel
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002472:Cell Transformation, Viral; D003062:Codon; D004247:DNA / Q000096:biosynthesis; D004854:Herpesvirus 4, Human; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011061:Poly A; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis*; D014158:Transcription, Genetic
D003062:Codon; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA