title: Turnover of the surface proteins of adult and third and fourth stage larval Brugia pahangi.
abstract: The turnover of surface proteins in adults, fourth-stage and third-stage larvae of Brugia pahangi was measured using [125I]iodosulfanilic acid. Groups of worms (n = 10 adult, 20 L4, 50 L3) were labelled and surgically implanted into the peritoneal cavity of naive jirds. The amount of radioactivity remaining on worms recovered over a 7-8 day period was determined. Adult females showed no significant loss of label during a 7 day period. The recovery of fourth-stage larvae was low but the counts per minute remaining on each group of larvae recovered over an 8 day period, encompassing the major part of the instar, did not fall below the limits of the standard deviation of the time 0 groups, indicating that no significant loss of surface label had occurred. Third-stage larvae showed a significant loss of 125I-labelled proteins prior to the third moult, although it was not confirmed that these proteins occur on the worm surface. Electrophoresis and autoradiography of labelled homogenates of adult, fourth and third stage larvae suggested that [125I]iodosulfanilic acid labels polypeptides of different molecular weights on each life cycle stage of B. pahangi.
authors.forename: E
authors.initials: E
authors.lastname: Marshall
authors.forename: R E
authors.initials: RE
authors.lastname: Howells
mesh_terms: D000818:Animals; D002009:Brugia / Q000254:growth & development / Q000378:metabolism*; D009033:Culicidae; D005260:Female; D005849:Gerbillinae; D007814:Larva; D008565:Membrane Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D010455:Peptides