Identification of Theiler's virus infected cells in the central nervous system of the mouse during demyelinating disease.
Theiler's virus is a picornavirus responsible for a persistent, demyelinating infection of mouse central nervous system. We examined the nature of infected cells during the course of this disease using a simultaneous immunoperoxidase-in situ hybridization assay. Cell types were identified with antigenic markers and infected cells were recognized by the presence of viral RNA. We found that, depending on the animal, approximately 10% of infected cells were migroglia-macrophages, 5 to 10% were astrocytes and 25 to 40% were oligodendrocytes. Approximately half of the infected cells could not be identified.
Aubert
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Départment de Virologie, UA CNRS 041157, Institut Pasteur, Paris, France.
Chamorro
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Brahic
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D000818:Animals; D001253:Astrocytes / Q000382:microbiology; D002256:Carbonic Anhydrases / Q000032:analysis; D003711:Demyelinating Diseases / Q000382:microbiology*; D004770:Enterovirus / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000382:microbiology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008264:Macrophages / Q000382:microbiology; D004680:Maus Elberfeld virus / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D009693:Nucleic Acid Hybridization; D009836:Oligodendroglia / Q000382:microbiology; D012367:RNA, Viral / Q000032:analysis; D013116:Spinal Cord / Q000382:microbiology*
D005904:Glial Fibrillary Acidic Protein; D012367:RNA, Viral; D002256:Carbonic Anhydrases