title: Induction of salivation in biting midges and mosquitoes, and demonstration of virus in the saliva of infected insects.
abstract: Culicoides biting midges and Aedes aegypti (Linnaeus) mosquitoes were induced to salivate by the topical application of pilocarpine, neostigmine, malathion and dimethoate; of these, malathion was the most effective. Drops of saliva produced by virus-infected midges and mosquitoes were shown to contain virus. The method could be used to demonstrate transmission in insects infected with a variety of pathogens.
authors.affiliation: Animal Virus Research Institute, Pirbright, Woking, Surrey.
authors.forename: J
authors.initials: J
authors.lastname: Boorman
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D001820:Bluetongue virus / Q000302:isolation & purification; D002519:Ceratopogonidae / Q000382:microbiology*; D007303:Insect Vectors / Q000382:microbiology*; D012463:Saliva / Q000382:microbiology; D012472:Salivation / Q000187:drug effects; D012672:Semliki forest virus / Q000302:isolation & purification