title: Serotypic classification of hantaviruses by indirect immunofluorescent antibody and plaque reduction neutralization tests.
abstract: Antisera prepared against 16 strains of hantaviruses isolated from patients with hemorrhagic fever with renal syndrome (HFRS) or from rodents captured in HFRS-endemic and nonendemic regions were titrated against Hantaan virus strain 76-118, Puumala virus strain Sotkamo, and Prospect Hill virus strain Prospect Hill-I by using the indirect immunofluorescent antibody and plaque reduction neutralization tests. Isolates fell into one of four distinct groups or serotypes. Serotype 1 included Apodemus-derived strains, serotype 2 included Rattus-derived strains, serotype 3 included Clethrionomys-derived strains, and serotype 4 included Microtus-derived strains. Serotypic classification of hantavirus infections was possible for humans and rodents in widely varied geographical areas, but in a few instances, sera from patients with HFRS did not conform to any of the four serotypes, suggesting the existence of as yet unidentified serotypes. A definitive serological classification of hantaviruses must await analysis of additional virus isolates.
authors.forename: P W
authors.initials: PW
authors.lastname: Lee
authors.forename: C J
authors.initials: CJ
authors.lastname: Gibbs
authors.forename: D C
authors.initials: DC
authors.lastname: Gajdusek
authors.forename: R
authors.initials: R
authors.lastname: Yanagihara
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D005455:Fluorescent Antibody Technique; D006476:Orthohantavirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D006480:Hemorrhagic Fever with Renal Syndrome / Q000382:microbiology; D006801:Humans; D009500:Neutralization Tests; D012328:RNA Viruses / Q000145:classification*; D051381:Rats; D012703:Serotyping / Q000379:methods*; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral