title: NMR evidence for an early framework intermediate on the folding pathway of ribonuclease A.
abstract: The presence of an early intermediate on the folding pathway of ribonuclease A has been demonstrated by a study of the exchange reaction between the backbone amide protons in the folding protein and solvent protons using rapid mixing techniques. A structural analysis of the intermediate by two-dimensional 1H-NMR is consistent with the framework model of protein folding in which stable secondary structure first forms the framework necessary for the subsequent formation of the complete tertiary structure.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, California 94305.
authors.forename: J B
authors.initials: JB
authors.lastname: Udgaonkar
authors.forename: R L
authors.initials: RL
authors.lastname: Baldwin
mesh_terms: D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011487:Protein Conformation; D011522:Protons; D012259:Ribonuclease, Pancreatic
chemical_list: D011522:Protons; D012259:Ribonuclease, Pancreatic