Macromolecular osteolytic factor synthesised by squamous carcinoma cell lines from the head and neck in vitro is interleukin 1.
Three human cell lines derived from oro-pharyngeal squamous cell carcinomas of the head were investigated for bone-resorbing activity in vitro. Culture media from all three spontaneously produced a non-dialysable osteolytic factor with activity in three in vitro assays for interleukin 1 (IL1), viz. the lymphocyte activating factor (LAF) assay, stimulation of collagenase synthesis by articular chondrocytes, and stimulation of prostaglandin E2 synthesis by fibroblasts. Addition of anti-human IL1 antibody to the culture media abolished all the bone-resorbing activity. Fractionation of the cell culture media by high performance liquid chromatography (HPLC) showed a single peak of activity in the chondrocyte assay with an apparent mol.wt of 15-17,000. This co-eluted with activity in a preparation of IL1 from rat peritoneal macrophage cultures. These results indicate that IL1 is responsible for the prostaglandin-independent bone resorbing activity synthesised by these cells in vitro, and may contribute to the bone destruction associated with the tumour.
Meghji
S
S
Joint Department of Oral and Maxillofacial Surgery, Institute of Dental Surgery and University College, London, UK.
Sandy
J R
JR
Scutt
A M
AM
Harvey
W
W
Carter
R L
RL
Harris
M
M
D001862:Bone Resorption; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002356:Cartilage / Q000201:enzymology; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D003012:Microbial Collagenase / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects; D011458:Prostaglandins E / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007375:Interleukin-1; D011458:Prostaglandins E; D014409:Tumor Necrosis Factor-alpha; D003012:Microbial Collagenase