Effect of metal containing compounds on superoxide release from phorbol myristate acetate stimulated murine peritoneal macrophages: inhibition by auranofin and spirogermanium.
A variety of metal containing compounds were examined for their ability to inhibit the respiratory burst of murine peritoneal macrophages. Auranofin (AF), a gold containing complex used in the treatment of rheumatoid arthritis, is a potent inhibitor of the macrophage respiratory burst. Ten rhodium, iridium, osmium and ruthenium containing complexes were inactive in inhibiting superoxide production. The only active nongold organometallic complex was spirogermanium which had an equivalent IC50 for activity as AF. The inhibitory activity of AF, but not spirogermanium, was reduced in the presence of the sulfhydryl reducing agent dithiothreitol. This suggests that interactions other than those with sulfhydryl groups may be involved in the action of spirogermanium.
Mirabelli
C K
CK
Smith Kline and French Laboratories, Philadelphia, PA.
Sung
C P
CP
Picker
D H
DH
Barnard
C
C
Hydes
P
P
Badger
A M
AM
D000818:Animals; D001310:Auranofin / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009942:Organometallic Compounds / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D013141:Spiro Compounds / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D009942:Organometallic Compounds; D013141:Spiro Compounds; D013438:Sulfhydryl Compounds; D013481:Superoxides; C026818:spirogermanium; D001310:Auranofin; D013755:Tetradecanoylphorbol Acetate