title: Role of exogenous hemin in the synthesis of hemoproteins and nonheme proteins during glucose repression in Saccharomyces cerevisiae.
abstract: Exogenous addition of hemin to glucose-repressed cells of Saccharomyces cerevisiae stimulates the incorporation of amino acid into cytoplasmic proteins twofold. There was no significant change in the synthesis of total cytoplasmic RNA whereas a 40% increase in the synthesis of poly(A)-containing RNA was observed upon hemin treatment. Cell-free translation of cytoplasmic mRNAs and immunoprecipitation analysis of the translated products with antibodies against subunit V of cytochrome oxidase and the alpha and beta subunits of F1-ATPase reveals that there is an eightfold enrichment of the mRNA for subunit V of cytochrome oxidase upon hemin treatment. The effect of hemin on the alpha and beta subunits of F1-ATPase is only marginal, suggesting a differential role for heme in the synthesis of hemoproteins and nonheme proteins during glucose repression.
authors.forename: G
authors.initials: G
authors.lastname: Gopalan
authors.forename: C
authors.initials: C
authors.lastname: Rajamanickam
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000096:biosynthesis; D005656:Fungal Proteins / Q000096:biosynthesis*; D005947:Glucose / Q000494:pharmacology*; D006418:Heme / Q000031:analogs & derivatives*; D006420:Hemeproteins / Q000096:biosynthesis*; D006427:Hemin / Q000494:pharmacology*; D014176:Protein Biosynthesis; D006180:Proton-Translocating ATPases / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D005656:Fungal Proteins; D006420:Hemeproteins; D012333:RNA, Messenger; D006418:Heme; D006427:Hemin; D003576:Electron Transport Complex IV; D006180:Proton-Translocating ATPases; D005947:Glucose