Cloning and expression in Escherichia coli of genes involved in the lysine pathway of Brevibacterium lactofermentum.
The Brevibacterium lactofermentum genes which complement Escherichia coli lysA and asd-1 mutants were identified, respectively, as a 1.9-kilobase PstI-ClaI fragment and a 2.5-kilobase PstI fragment by cloning into pBR325. Southern blot transfers show hybridization to chromosomal fragments of identical size. The putative B. lactofermentum asd and lysA products are 44 and 48 kilodaltons, respectively.
Márquez
G
G
Sousa
J M
JM
Sánchez
F
F
D001223:Aspartate-Semialdehyde Dehydrogenase / Q000235:genetics; D001951:Brevibacterium / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008239:Lysine / Q000096:biosynthesis / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D008715:Methionine; D001223:Aspartate-Semialdehyde Dehydrogenase; D008239:Lysine