Kainic acid-induced release of serotonin from OFF-bipolar cells in the turtle retina.
The immunofluorescence of antibody-labelled serotonin was used as an indicator for the amount of intracellular serotonin. Incubation in Ringer solution containing serotonin and pargyline increased the immunofluorescence which was subsequently decreased in a calcium-dependent manner by incubation in high-potassium Ringer. If preloaded retinas were exposed to 10 microM kainic acid, the immunofluorescence decreased in a time-dependent way. This kainic acid-induced release was blocked by piperidine dicarboxylic acid. These results support the idea that serotonin is a transmitter in OFF-bipolar cells.
Weiler
R
R
Schütte
M
M
D000818:Animals; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000494:pharmacology*; D009474:Neurons / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000502:physiology; D012160:Retina / Q000378:metabolism* / Q000502:physiology; D012701:Serotonin / Q000378:metabolism* / Q000502:physiology; D009435:Synaptic Transmission; D014426:Turtles / Q000378:metabolism*
D018377:Neurotransmitter Agents; D012701:Serotonin; D007608:Kainic Acid