Calcium-dependence of somatostatin binding to receptors.
Binding of 125I-[Tyr11] somatostatin (S14) and 125I-[Tyr1]S14 has been studied in pancreatic acini and cerebral cortex. Ca2+-dependence of somatostatin binding to receptors was observed only with the highly non degradable iodinated analog 125I-[Tyr11] somatostatin but not with 125I-[Tyr1] somatostatin. The inhibitory activity of S14 on secretin-stimulated cAMP cellular content was decreased when Ca2+ concentration in the medium was reduced to 30 nM, indicating that the Ca2+ dependence of somatostatin binding to receptors is also present with the native peptide.
Susini
C
C
Esteve
J P
JP
Vaysse
N
N
Ribet
A
A
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D010179:Pancreas / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017481:Receptors, Somatostatin; D013004:Somatostatin / Q000378:metabolism*
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D017481:Receptors, Somatostatin; D013004:Somatostatin; D000242:Cyclic AMP; D002118:Calcium