Use of dodecyl sulfate as an esterase inhibitor before gas-chromatographic determination of labile beta-adrenoceptor blocking drugs.
Sodium dodecyl sulfate (SDS) was introduced as an esterase inhibitor in the determination of two extremely labile, ultrashort-acting beta-blockers-esmolol and flumolol--in whole blood. SDS was much more efficient than previously used agents in preventing hydrolysis. The assay comprises solvent extraction, perfluoroacylation, and capillary gas-chromatography with electron-capture detection. The limit of detection (the concentration at which the coefficient of variation is 10-15%) for the drugs is 5 nmol/L of whole blood.
Holm
G
G
Kylberg-Hanssen
K
K
Svensson
L
L
D000319:Adrenergic beta-Antagonists / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D004950:Esterases / Q000037:antagonists & inhibitors*; D006801:Humans; D011412:Propanolamines / Q000097:blood*; D012967:Sodium Dodecyl Sulfate
D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; D012967:Sodium Dodecyl Sulfate; C045634:flumolol; D004950:Esterases; C036604:esmolol