Saposin D: a sphingomyelinase activator.
Saposin D, a newly discovered heat-stable, 10 kDa glycoprotein, was isolated from Gaucher spleen and purified to homogeneity. Chemical sequencing from its amino terminus demonstrated colinearity between its amino acid sequence and the deduced amino acid sequence of the fourth domain of prosaposin, the precursor of saposin proteins. Saposin D specifically stimulates acid sphingomyelinase but has no significant effect on the other hydrolases tested.
Morimoto
S
S
Department of Neurosciences, School of Medicine, University of California, San Diego, La Jolla 92093.
Martin
B M
BM
Kishimoto
Y
Y
O'Brien
J S
JS
D004789:Enzyme Activation; D005260:Female; D005776:Gaucher Disease / Q000378:metabolism; D005962:Glucosylceramidase / Q000378:metabolism; D006023:Glycoproteins / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D049231:Saposins; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D006023:Glycoproteins; D049231:Saposins; D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D005962:Glucosylceramidase