title: DNA hybridization assay for congenital cytomegalovirus infection.
abstract: An in vitro DNA hybridization assay was used to test 281 newborns for congenital infection with cytomegalovirus. The assay utilized an abbreviated method for DNA preparation and a dot blot assay that provided good sensitivity (100%) and specificity (98.9%) when compared with standard tissue culture, yet substantially reduced the total time of analysis. This assay would be a useful adjunct to tissue culture to diagnose newborns with congenital infection with cytomegalovirus.
authors.affiliation: Department of Pathology, Baylor College of Medicine, Houston, Texas.
authors.forename: G J
authors.initials: GJ
authors.lastname: Buffone
authors.forename: G J
authors.initials: GJ
authors.lastname: Demmler
authors.forename: C M
authors.initials: CM
authors.lastname: Schimbor
authors.forename: M D
authors.initials: MD
authors.lastname: Yow
mesh_terms: D003586:Cytomegalovirus Infections / Q000151:congenital / Q000175:diagnosis*; D004279:DNA, Viral / Q000652:urine*; D006801:Humans; D007231:Infant, Newborn; D009693:Nucleic Acid Hybridization
chemical_list: D004279:DNA, Viral