title: [SH-groups of NAD kinase from the rabbit liver].
abstract: Two SH-groups per enzyme subunit have been identified in the native preparation of rabbit liver NAD kinase, using DTNB. The titration curve is biphasic; one SH-group is modified at each step. There is a strict correlation between the loss of the enzyme activity and the rate of modification of fast and slow SH-groups. Substrates afford only a partial protection of NAD kinase against the DTNB-induced inactivation. The data obtained suggest that two SH-groups of NAD kinase are essential for the enzyme activity; however, these groups are not directly involved in the active center formation.
authors.forename: E R
authors.initials: ER
authors.lastname: Bulygina
authors.forename: O L
authors.initials: OL
authors.lastname: Voronina
authors.forename: M S
authors.initials: MS
authors.lastname: Tsyplenkova
authors.forename: V I
authors.initials: VI
authors.lastname: Telepneva
mesh_terms: D000818:Animals; D002384:Catalysis; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology; D010770:Phosphotransferases / Q000032:analysis*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011817:Rabbits; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D013438:Sulfhydryl Compounds; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase
vernacular_title: Issledovanie SH-grupp NAD-kinazy iz pecheni krolika.