Transformation of human cells by a polyomavirus containing complementing JCV and RFV genomes.
Molecularly cloned viral DNA from late RFV (L-RFV) and early JCV (E-JCV) were transfected into human fetal brain (HFB) cells and complementation was demonstrated. A new infectious virus (E-JCV/L-RFV) was produced. Infection resulted in partial transformation of HFB and human embryonic kidney cells. No transformation was observed with EL-JCV or wtJCV. The transformants contained T-antigen and had a lifespan similar to SV40-transformed human cells but failed to express some phenotypes of transformation. All transformants contained E-JCV viral DNA, usually both integrated and episomal. Although no L-RFV DNA was present in the transformants, L-RFV appears to play a role in the initiation of transformation.
O'Neill
F J
FJ
Research Service, VA Medical Center, Salt Lake City, Utah.
Renzetti
L
L
Miller
T H
TH
Stevens
R
R
D001739:BK Virus / Q000235:genetics*; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D005816:Genetic Complementation Test; D006801:Humans; D006824:Hybridization, Genetic; D007577:JC Virus / Q000235:genetics*; D010641:Phenotype; D011120:Polyomavirus / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
D004279:DNA, Viral