CNS tissue levels of dynorphin-A immunoreactivity and the anorexia associated with sodium chloride imbibition in the rat.
Forced imbibition of increasing concentrations of sodium chloride (NaCl) in rats reduced daytime 2-deoxy-D-glucose (2-DG) induced feeding in a concentration dependent manner. Pituitary neurointermediate lobe (NIL) levels of immunoreactive (ir)-dynorphin-A 1-17 and 1-8 were also decreased by the NaCl regimen in a concentration dependent manner. However, there was no significant association between the reduction of NIL dynorphin levels and the suppression of 2-DG induced feeding on a within-animal basis. NaCl imbibition did not affect levels of either ir-dynorphin-A 1-17 or 1-8 in the hypothalamus, cerebral cortex, hippocampus, medulla/pons or anterior pituitary. Neither the acute changes following 2-DG administration, nor the comparison of ir-dynorphin-A 1-8/1-17 ratios appeared useful for the assessment of dynorphin-A turnover. Thus, the present results did not support the hypothesis that anorexia of NaCl treated animals results from the depletion of dynorphin-A.
Bryant
H U
HU
Yim
G K
GK
Malven
P V
PV
D000595:Amino Acid Sequence; D000818:Animals; D000855:Anorexia / Q000378:metabolism*; D001066:Appetite / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D004399:Dynorphins / Q000378:metabolism*; D005247:Feeding Behavior / Q000187:drug effects*; D001068:Feeding and Eating Disorders / Q000378:metabolism*; D006801:Humans; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology*
D012965:Sodium Chloride; D004399:Dynorphins; D003847:Deoxyglucose