Enterotoxigenic Escherichia coli (ETEC) and Klebsiella pneumoniae isolated from dogs with diarrhoea.
Faecal samples from 148 dogs with diarrhoea and from 15 healthy dogs were cultured for bacterial pathogens with enterotoxigenic properties. The aim of the study was to define the toxin profile (production of heat-labile [LT] and heat-stable [ST] toxins) and possible surface fimbrial antigens. Enterotoxigenic bacteria were isolated from 6 (4.1%) dogs with diarrhoea, four of these were Escherichia coli and two were Klebsiella pneumoniae. The E. coli strains and K. pneumoniae strains were producing both LT and ST toxins. The LT toxin from these strains was not neutralized by human anti-LT serum or anti-choleragen and did not cause coagglutination with Staphylococcus aureus coated with anti-human-LT. This suggests that the LT toxin produced by these canine isolates is non-identical to LT toxin from human strains. Three of the ETEC strains were haemagglutinating and showed surface hydrophobic properties. Electron microscopy showed that canine ETEC isolates possessed fimbriae of two different types: thick (5-5.5 nm) and thin (2-3 nm).
Olson
P
P
Hedhammar
A
A
Faris
A
A
Krovacek
K
K
Wadström
T
T
D000818:Animals; D000942:Antigens, Bacterial; D001427:Bacterial Toxins / Q000096:biosynthesis; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D010861:Fimbriae, Bacterial; D006384:Hemagglutination; D007710:Klebsiella Infections / Q000382:microbiology / Q000662:veterinary; D007711:Klebsiella pneumoniae / Q000302:isolation & purification / Q000378:metabolism*
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli