Detection and purification of bovine herpesvirus 1 glycoproteins by lectin affinity.
A procedure for detection and purification of bovine herpesvirus 1 glycoproteins by their affinity for lectins is described. Wheat germ lectin, Limulus polyphemus lectin and concanavalin A, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. These lectins have also been used for affinity-purification of sets of glycoproteins. The procedure is easy, rapid, cheap and, when biotin is used, non-radioactive.
Badia
M
M
Institut de Biologia Fonamental V. Villar Palasí, Universitat Autónoma de Barcelona, Spain.
Querol
E
E
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification; D007242:Herpesvirus 1, Bovine / Q000032:analysis*; D037102:Lectins; D008970:Molecular Weight; D014764:Viral Proteins / Q000302:isolation & purification*
D006023:Glycoproteins; D037102:Lectins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins