Single acetylcholine channel currents recorded from Xenopus myocytes are influenced by the glass of the patch pipette.
The type of glass used to fabricate patch pipettes influences the kinetic properties of single-channel currents activated by acetylcholine in Xenopus myocytes. In patches formed with hard glass pipettes, the opening rate decreased in a time-dependent manner. Such decrease was most pronounced for the low conductance channel. In addition, longer duration events of this form of channel dropped out faster than shorter duration events and, as a consequence, the apparent channel open time became shorter during the recording period. These effects were not observed in patches formed with soft glass pipettes.
Rojas
L
L
Institute of Neurobiology, University of Puerto Rico Medical Sciences Campus, San Juan.
Zuazaga
C
C
D000818:Animals; D005898:Glass; D008564:Membrane Potentials; D009469:Neuromuscular Junction / Q000502:physiology*; D011950:Receptors, Cholinergic / Q000502:physiology*; D009435:Synaptic Transmission; D014982:Xenopus laevis
D011950:Receptors, Cholinergic