125I uptake by FRTL5 cells: a screening test to detect pregnant women at risk of giving birth to hypothyroid infants.
Thyroid-growth-blocking antibodies are present in large proportion of mothers who give birth to hypothyroid infants. In order to devise a prenatal screening test to replace the time-consuming cytobiochemical assay for measurement of the maternal antibodies, a cloned rat-thyroid cell-line (FRTL5) was used. Several approaches that had been tried previously had proved fruitless--eg, in FRTL5 cells immunoglobulin of mothers of affected infants did not displace 125I-labelled TSH from its receptor, did not block TSH-induced increase of cyclic AMP, and did not block TSH-induced 3H-thymidine incorporation. By use of 125I uptake by the cells, mothers of the hypothyroid children were readily distinguished from normal female subjects and normal pregnant women.
Dussault
J H
JH
Bernier
D
D
D000818:Animals; D002460:Cell Line; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis*; D006801:Humans; D007037:Hypothyroidism / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000378:metabolism; D007231:Infant, Newborn; D007457:Iodine Radioisotopes; D011247:Pregnancy; D051381:Rats; D012306:Risk; D013972:Thyrotropin / Q000378:metabolism
D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D013972:Thyrotropin