title: DNA hybridization for detection of human cytomegalovirus in bronchoalveolar lavage and pharynx biopsy.
abstract: A simplified hybridization procedure was used for detection of cytomegalovirus (CMV) in human specimens. The probe was a 32P-labelled cloned DNA fragment of CMV strain AD169. This probe did not hybridize to DNA from uninfected cells or other herpesviruses (herpes simplex virus, Epstein-Barr virus). Specific hybridization was obtained with unselected bronchoalveolar lavage specimens, but the sensitivity of the test (33%) was lower than that of culture (80%) and immunofluorescence (60%) assays which are routinely performed in our laboratory. The detection procedure was also carried out with pharynx biopsy specimens which had been kept frozen at -70 degrees C. CMV DNA was detected in 14% of tumour specimens and only in 1.7% of control specimens (p less than 0.05). The indications of DNA hybridization for CMV diagnosis are discussed.
authors.affiliation: Laboratoire de Virologie, Hôpital Trousseau, Paris.
authors.forename: H
authors.initials: H
authors.lastname: Agut
authors.forename: P
authors.initials: P
authors.lastname: Deny
authors.forename: E
authors.initials: E
authors.lastname: Rousseau
authors.forename: A
authors.initials: A
authors.lastname: Garbarg-Chenon
authors.forename: C
authors.initials: C
authors.lastname: Beliveau
authors.forename: F
authors.initials: F
authors.lastname: Chabolle
authors.forename: H
authors.initials: H
authors.lastname: Chouard
authors.forename: F
authors.initials: F
authors.lastname: Bricout
authors.forename: J C
authors.initials: JC
authors.lastname: Nicolas
mesh_terms: D001992:Bronchoalveolar Lavage Fluid; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D015342:DNA Probes; D005455:Fluorescent Antibody Technique; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010614:Pharynx / Q000382:microbiology
chemical_list: D015342:DNA Probes