title: A kinetic study of [3H]-dexamethasone binding to chick erythrocytes.
abstract: The chick erythrocyte has about 300 specific glucocorticoid binding sites per cell. The apparent dissociation constant at 20 degrees C was 0.31 nM after 1 hr incubation and decreased to 0.12 nM after 8 hr incubation. The association rate constant of [3H]-dexamethasone binding to chick erythrocytes was higher than that reported for goat mononuclear leukocytes. The dissociation of [3H]-dexamethasone bound to erythrocytes at 20 degrees C consisted of two components which are similar to the values reported for goat mononuclear leukocytes.
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: N
authors.initials: N
authors.lastname: Ohsawa
authors.forename: F
authors.initials: F
authors.lastname: Takaku
mesh_terms: D000818:Animals; D001665:Binding Sites; D002645:Chickens; D003907:Dexamethasone / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male
chemical_list: D003907:Dexamethasone