Tumor location using F(ab')2 mu from a monoclonal IgM antibody: pharmacokinetics.
A monoclonal IgM antibody (anti-SSEA-1) and its divalent antigen-binding peptic fragment [F(ab')2 mu] were compared as in vivo tumor localization reagents in mouse teratocarcinomas. F(ab')2 mu is cleared more rapidly than whole antibody from the whole body, blood, and all tested organs (t1/2 for whole antibody approximately 18 hr; t1/2 for F(ab')2 mu, 12 hr). A corresponding average improvement in tumor-to-tissue ratio is observed 48 hr after injection and earlier. However, the affinity of the F(ab')2 mu for antigen is much lower, and a smaller fraction of the antibody fragment is retained in the tumor than with whole antibody. The fragment was not retained by animals bearing nonantigenic tumors.
Ballou
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Reiland
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Levine
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Knowles
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Hakala
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D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D007457:Iodine Radioisotopes; D007700:Kinetics; D016256:Lewis X Antigen; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging*; D010954:Plasmacytoma / Q000000981:diagnostic imaging; D011877:Radionuclide Imaging; D013724:Teratoma / Q000000981:diagnostic imaging; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006017:Glycolipids; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D007457:Iodine Radioisotopes; D016256:Lewis X Antigen