Yeast adenylate kinase is transcribed constitutively from a promoter in the short intergenic region to the histone H2A-1 gene.
Yeast mitochondrial adenylate kinase (high molecular mass form, gene locus: AKY2) is encoded on chromosome IV of the same DNA strand as histone H2A-1. The nontranslated intergenic region spans 560 bp, the nontranscribed spacer can be estimated to comprise at most 300 bp. The TATA-box sequence is contained in a striking environment consisting of 20 alternating pyrimidines and purines. The AKY2 transcript is made constitutively: (i) the cellular mRNA concentration does not vary significantly with either growth conditions or elapse of the cell cycle; (ii) beta-galactosidase activity is about constant in yeast cells grown on various carbon sources after transformation with AKY2-promoter/lacZ fusions; (iii) primer elongation analysis shows that utilization of 5 initiation sites is qualitatively and quantitatively independent of the growth conditions and the carbon source used; (iv) Western blot analysis and adenylate kinase activity measurements indicate the absence of post-transcriptional controls as well.
Oechsner
U
U
Institute for Genetics and Microbiology, München, FRG.
Magdolen
V
V
Zoglowek
C
C
Häcker
U
U
Bandlow
W
W
D000263:Adenylate Kinase / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000201:enzymology; D004271:DNA, Fungal / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006657:Histones / Q000235:genetics*; D015151:Immunoblotting; D007527:Isoenzymes / Q000235:genetics*; D007763:Lac Operon; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010770:Phosphotransferases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D004271:DNA, Fungal; D006657:Histones; D007527:Isoenzymes; D012331:RNA, Fungal; D012333:RNA, Messenger; D010770:Phosphotransferases; D000263:Adenylate Kinase; D001616:beta-Galactosidase