title: Tolerance of axenically cultured Entamoeba histolytica and Giardia lamblia to a variety of antimicrobial agents.
abstract: Twelve antimicrobial agents were tested for use in axenic cultures of Entamoeba histolytica and Giardia lamblia. Ten did not inhibit parasite growth at concentrations of at least two-fold above the effective antibacterial or antifungal levels. We have found the combination of piperacillin (500 micrograms/ml) plus amikacin (125 micrograms/ml) to be useful in axenization of a new strain of E. histolytica, eradication of multiply drug-resistant contaminants in cultures and prevention of bacterial growth in experiments with non-sterile components.
authors.forename: M J
authors.initials: MJ
authors.lastname: Gault
authors.forename: D S
authors.initials: DS
authors.lastname: Reiner
authors.forename: F D
authors.initials: FD
authors.lastname: Gillin
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004748:Entamoeba histolytica / Q000187:drug effects*; D005872:Giardia / Q000187:drug effects*; D010403:Penicillin Resistance; D010878:Piperacillin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000583:Amikacin; D010878:Piperacillin