Collagenase synthesis in clonal monkey kidney epithelial cells (JTC-12 cells).
Cells of clone JTC-12 derived from monkey kidney epithelial cells were found to produce latent collagenase in serum-containing cultures. Collagenase production by JTC-12 cells was stimulated more than ten-fold with the addition of 12-0-tetradecanoyl-phorbol-13-acetate. The JTC-12 cell collagenase was purified 2,370-fold over the conditioned medium to a specific activity of 5,940 units/mg with a yield of 79% by heparin-Sepharose, ion-exchange and immunoadsorption chromatography. Thus the JTC-12 cell culture appears to be a useful model for studying the initial degradation of surrounding connective tissues by epithelial tissue in carcinogenesis.
Sakamoto
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Laboratory for the study of Connective Tissue Metabolism, Harvard School of Dental Medicine, Boston, Massachusetts 02115.
Yongue
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A
Sakamoto
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M
D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002999:Clone Cells; D004848:Epithelium; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D003012:Microbial Collagenase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight
D003012:Microbial Collagenase