Macrosorb Kieselguhr-agarose composite adsorbents. New tools for downstream process design and scale up. Scientific note.
Incompressible Macrosorb composite adsorbents, while retaining all the desirable properties of traditional agarose-based hydrogel media, overcome the operational limitations imposed by the use of soft hydrogels: They permit useful application of fast flow rates without restrictions on bed depth and they can be used in fluidized bed mode. Considerations which are important when contemplating scaled-up processing are discussed. A comparative cost estimate for a production process for extracting albumin from bovine serum in column equipment illustrates the various advantages which may be exploited when using a composite adsorbent in place of a conventional soft gel equivalent.
Bite
M G
MG
Sterling Organics R&D, Newcastle upon Tyne, England.
Berezenko
S
S
Reed
F J
FJ
Derry
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L
D000327:Adsorption; D007692:Diatomaceous Earth; D012685:Sepharose; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D012822:Silicon Dioxide
D012710:Serum Albumin, Bovine; D007692:Diatomaceous Earth; D012822:Silicon Dioxide; D012685:Sepharose