Isolation of the outer membrane components of Bordetella pertussis which enhance the immunogenicity of Haemophilus influenzae type b capsular polysaccharide polyribosyl ribitol phosphate.
The outer membrane (OM) component(s) from Bordetella pertussis which potentiated the antigenicity of purified Haemophilus influenzae type b capsular polysaccharide, polyribosyl ribitol phosphate (PRP), has been isolated and partially characterized. The OM was isolated from spheroplasting medium by precipitating at pH 5.0; fractionation was carried out by dissolving the crude OM in Triton X-100 followed by selective precipitation of OM in 50% ethanol. Further purification of OM was accomplished by DEAE-Sepharose 6BCL and Sephacryl S-300 chromatography. The biochemical composition and protein profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of various OM preparations have been examined. Combined vaccines consisting of OM components and PRP were given to young Sprague-Dawley rats, and the serum antibody to PRP was measured by an [3H]PRP binding assay. The purified OM containing mainly a 30,000-dalton protein, but not purified lipopolysaccharide or leukocytosis-promoting toxin, exhibited strong enhancement of PRP immunogenicity.
Monji
N
N
Stebbins
M R
MR
McCoy
D W
DW
Kuo
J S
JS
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D002462:Cell Membrane / Q000032:analysis / Q000276:immunology; D004731:Endotoxins / Q000032:analysis; D006193:Haemophilus influenzae / Q000276:immunology*; D007964:Leukocytosis / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008970:Molecular Weight; D011134:Polysaccharides / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D051381:Rats
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004731:Endotoxins; D008070:Lipopolysaccharides; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C008984:polyribitol phosphate