title: Removal of Arg1 and Phe22 from CLIP (ACTH18-39) by rodent pituitary and blood peptidases.
abstract: The major product on incubation of CLIP (ACTH18-39) with rat and mouse serum, rat plasma and whole blood, and soluble extracts of rat pituitary is [des-Arg1]-CLIP (ACTH19-39) while [des-Phe22]-CLIP (ACTH18-38) is the major product with pituitary particulate fraction. In both cases, p-chloromercuribenzoate-sensitive, metal-dependent peptidase activity appears to be responsible for the cleavage. The serum enzyme may be related to proline aminopeptidase. Material coeluting with [des-Arg]-CLIP on two HPLC solvent gradients is present in the superfusion media from neurointermediate lobes of genetically obese (ob/ob) mice but is not present in acid extracts of the lobe. This suggests that postsecretory processing of CLIP may involve removal of the N-terminal Arg residue.
authors.affiliation: MRC Neuroendocrinology Unit, Newcastle General Hospital.
authors.forename: J R
authors.initials: JR
authors.lastname: McDermott
authors.forename: J A
authors.initials: JA
authors.lastname: Biggins
authors.forename: A I
authors.initials: AI
authors.lastname: Smith
authors.forename: A M
authors.initials: AM
authors.lastname: Gibson
authors.forename: A B
authors.initials: AB
authors.lastname: Keith
authors.forename: J A
authors.initials: JA
authors.lastname: Edwardson
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine; D053497:Corticotropin-Like Intermediate Lobe Peptide; D051379:Mice; D008820:Mice, Obese; D010446:Peptide Fragments / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000097:blood / Q000378:metabolism*; D010649:Phenylalanine; D010902:Pituitary Gland / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D011480:Protease Inhibitors; D010649:Phenylalanine; D053497:Corticotropin-Like Intermediate Lobe Peptide; D000324:Adrenocorticotropic Hormone; D001120:Arginine; D010447:Peptide Hydrolases