LC/MS determination of bromazepam, clopenthixol, and reserpine in serum of a non-fatal case of intoxication.
Combined liquid chromatography and mass spectrometry (LC/MS) with a moving belt interface can be used as a rapid method for the determination of bromazepam, clopenthixol, and reserpine in serum samples obtained from cases of acute overdoses with combinations of these drugs. Low resolution detection limits are about 100 pg for the three drugs, while in high resolution mode the detection limit for bromazepam is shown to be at least 35 pg. Accurate masses were obtained in a serum sample within 5 ppm using high voltage scanning over a narrow mass range for about 10 ng of bromazepam and clopenthixol, respectively. Chemical deactivation of the belt was shown to effectively reduce memory effects and to improve the desorption characteristics of the belt leading to higher yields of evaporated intact molecules.
Tas
A C
AC
van der Greef
J
J
ten Noever de Brauw
M C
MC
Plomp
T A
TA
Maes
R A
RA
Hohn
M
M
Rapp
U
U
D014151:Anti-Anxiety Agents / Q000097:blood*; D001960:Bromazepam / Q000097:blood* / Q000506:poisoning; D002853:Chromatography, Liquid; D003006:Clopenthixol / Q000097:blood* / Q000506:poisoning; D006801:Humans; D013058:Mass Spectrometry; D012110:Reserpine / Q000097:blood* / Q000506:poisoning; D013892:Thioxanthenes / Q000097:blood*
D014151:Anti-Anxiety Agents; D013892:Thioxanthenes; D012110:Reserpine; D003006:Clopenthixol; D001960:Bromazepam