OR3 operator of bacteriophage lambda in a 23 base-pair DNA fragment: sequence-specific 1H NMR assignments for the non-labile protons and comparison with the isolated 17 base-pair operator.
Sequence-specific 1H NMR assignments are presented for a non-selfcomplementary 23-base-pair DNA duplex of molecular weight 15,000 daltons, containing the OR3 repressor binding site of bacteriophage lambda as the central core. The NMR techniques used were mainly phase-sensitive two-dimensional NOE and 2Q spectroscopy, the latter to overcome overlap problems within the spectral region of the deoxyribose spin-systems. Direct sequential NOE connectivities are observed between adenine 2 H and deoxyribose 1' protons. We propose the use of these connectivities as a check of the assignments of C1' and A2 protons, which have independently been derived via other assignment pathways.
Grütter
R
R
Institut für Molekularbiologie und Biophysik, ETH-Hönggerberg, Zürich, Switzerland.
Otting
G
G
Wüthrich
K
K
Leupin
W
W
D010582:Bacteriophage lambda / Q000235:genetics*; D001482:Base Composition; D004279:DNA, Viral / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D009875:Operator Regions, Genetic; D011522:Protons
D004279:DNA, Viral; D011522:Protons