cDNA cloning and sequence determination of pig gastric (H+ + K+)-ATPase.
Complementary DNA to pig gastric mRNA encoding (H+ + K+)-ATPase was cloned, and its amino acid sequence was deduced from the nucleotide sequence. The enzyme contained 1034 amino acid residues (Mr. 114,285) including the initiation methionine. The sequence of pig (H+ + K+)-ATPase was highly homologous with that of the corresponding enzyme from rat, but had high degree of synonymous codon changes. Potential sites of phosphorylation by cAMP-dependent protein kinase and N-linked glycosylation sites were identified. The amino terminal region contained a lysine-rich sequence similar to that of the alpha subunit of (Na+ + K+)-ATPase, although a cluster of glycine residues was inserted into the sequence of the (H+ + K+)-ATPase. As the pig enzyme is advantageous for biochemical studies, the information of the primary structure is useful for further detailed studies.
Maeda
M
M
Department of Organic Chemistry and Biochemistry, Osaka University, Japan.
Ishizaki
J
J
Futai
M
M
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D017506:H(+)-K(+)-Exchanging ATPase; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013552:Swine
D004247:DNA; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase