(-)-Adrenaline-induced, calcium-dependent phosphorylation of proteins in human platelets.
In human platelets, adrenaline stimulated, approximately four-fold, as compared with controls, the phosphorylation of primarily two proteins of apparent molecular weights of 20,000 and 40,000, respectively. Maximum phosphorylation occurred after incubation for 1 min and was inhibited by the addition of either yohimbine, prostaglandin E1, or EGTA. Phosphorylation of the two proteins was accompanied by diacylglycerol formation. The (-)-adrenaline-induced phosphorylation of proteins corresponds to the activation of a calcium-dependent protein kinase partially purified by DEAE-cellulose and Sephadex G150 column chromatography. The enzymatic activity was modulated by addition of (-)-adrenaline and CaCl2, by diolein, and in the presence of membranes or phosphatidylinositol but not phosphatidylethanolamine and phosphatidylcholine. A phospholipid-dependent reaction appears to be involved in the molecular mechanism of action of adrenaline.
Block
L H
LH
Jaksche
H
H
Erne
P
P
Bolli
P
P
Bühler
F R
FR
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D003285:Contractile Proteins / Q000097:blood* / Q000302:isolation & purification; D004075:Diglycerides / Q000096:biosynthesis; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C; D011494:Protein Kinases / Q000096:biosynthesis / Q000302:isolation & purification; D015016:Yohimbine / Q000494:pharmacology
D000317:Adrenergic alpha-Antagonists; D003285:Contractile Proteins; D004075:Diglycerides; D010761:Phosphorus Radioisotopes; D015016:Yohimbine; D011494:Protein Kinases; D011493:Protein Kinase C; D002118:Calcium; D004837:Epinephrine