[Detection of hepatitis A virus RNA by molecular hybridization].
Hepatitis A virus (HAV) was propagated in continuous rhesus monkey embryo kidney cells (FRhK-4 line). HAV RNA extracted with phenol from purified HAV pretreated with proteinase K was used for molecular cloning experiments. Two radioactive plasmids, pHAV-23 and pHAV-5p, containing HAV cDNA fragments complementary to structural and nonstructural parts of HAV genome, respectively, were found to be useful for discrimination between mature virions and subviral structures. HAV cDNA-RNA hybridization seems to be 10 times more sensitive than the enzyme immunoassay (ELISA).
Kusov
Iu Iu
IuIu
Kazachkov
Iu A
IuA
Grabko
V I
VI
Nastashenko
T A
TA
Balaian
M S
MS
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005243:Feces / Q000382:microbiology; D006506:Hepatitis A / Q000175:diagnosis; D006507:Hepatovirus / Q000235:genetics*; D006801:Humans; D007124:Immunoenzyme Techniques; D008253:Macaca mulatta; D008992:Monkey Diseases / Q000175:diagnosis; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D015347:RNA Probes; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D014776:Virus Cultivation
D004279:DNA, Viral; D010761:Phosphorus Radioisotopes; D015347:RNA Probes; D012367:RNA, Viral; D004247:DNA
Obnaruzhenie RNK virusa gepatita A metodom molekuliarnoĭ gibridizatsii.