[Effect of blood plasma and serum and its fractions on the activity of the sodium channels in neuroblastoma cells].
Blood plasma, serum and its fractions containing components of different molecular masses obtained by dialysis and gradual ultrafiltration were tested for their action on the sodium currents of voltage clamped internally perfused neuroblastoma cells. Blood plasma free of platelet factor 4, serum and its fractions containing components of molecular masses more than 50 kD produced an increase in sodium channel currents and shifts in activation and inactivation curves along the voltage axis towards more negative or positive potentials, respectively. Moreover, the serum deficient in components with the molecular masses less than 50 kD produced a more prominent effect on all the parameters tested. Thus, the low-molecular fractions were suggested to exert an inhibitory activity on the stimulatory effects produced by the high-molecular components of the serum.
Zubov
A N
AN
Pisareva
L N
LN
Ivanova
G I
GI
Nosyreva
E D
ED
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008970:Molecular Weight; D009447:Neuroblastoma / Q000503:physiopathology*; D010949:Plasma / Q000502:physiology*; D015222:Sodium Channels / Q000187:drug effects* / Q000502:physiology; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D015222:Sodium Channels
Vliianie plazmy, syvorotki krovi i ee fraktsiĭ na aktivnost' natrievykh kanalov neĭroblastomy.