Studies on the immobilization of glucuronidase (Part 1). Covalent immobilization on various carriers (a comparison).
beta-Glucuronidase (EC 3.2.1.31) was immobilized on various organic and inorganic carriers by different methods. Optimum coupling conditions have been worked out. The immobilization were characterized and compared to each other. Parameters resulting in most stable preparations with high activities are discussed.
Rapatz
E
E
Institut für Allgemeine Biochemie, Universität Wien, Austria.
Travnicek
A
A
Fellhofer
G
G
Pittner
F
F
D003488:Cyanogen Bromide; D004790:Enzyme Induction; D004800:Enzymes, Immobilized / Q000032:analysis*; D005898:Glass; D005966:Glucuronidase / Q000032:analysis*; D005976:Glutaral / Q000378:metabolism; D010084:Oxidation-Reduction; D010504:Periodic Acid; D012685:Sepharose
D004800:Enzymes, Immobilized; D010504:Periodic Acid; D012685:Sepharose; C009288:metaperiodate; D005966:Glucuronidase; D003488:Cyanogen Bromide; D005976:Glutaral