Acquisition of repertoires of suppressor T cells under the influence of macrophages.
Acquisition of repertoires and genetic restriction specificities of suppressor T cells (Ts) and their factors were studied by using full allogeneic radiation bone marrow chimera and H-2 congenic pairs, B10.A(3R) and B10.A(5R), which received conventional or cloned macrophages by cell transfer. Suppressor T-cell factor (TsF) from C3H----C57BL/6 or C57BL/6----C3H chimera suppressed only donor but not host-type responses of either C3H or C57BL/6, in an antigen-specific fashion. However, if chimera mice were given conventional or cloned macrophages of the host type, the chimera TsF in turn suppressed both the responses of C3H and C57BL/6 mice but not those of the third party, BALB/c, indicating that macrophages are responsible for the acquisition of host restriction specificity. Similarly, B10.A(5R) mice developed I-Jb restricted Ts or TsF when the B10.A(3R) macrophage cell line was injected at the time of antigen priming. The reverse was also true. B10.A(3R) mice did generate I-Jk restricted Ts when they received the B10.A(5R) macrophage cell line. Thus, the results clearly demonstrated that B10.A(3R) or B10.A(5R) mice potentially possessed their ability to express both I-Jk and I-Jb determinants and that repertoires and genetic restriction specificity of Ts and their TsF were acquired at a macrophage level at the time of antigen-priming.
Soejima
T
T
Division of Molecular Immunology, School of Medicine, Chiba University, Japan.
Nagayama
A
A
Sado
T
T
Taniguchi
M
M
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D016026:Bone Marrow Transplantation; D002461:Cell Line, Transformed; D006433:Hemocyanins; D006649:Histocompatibility Antigens / Q000276:immunology; D008264:Macrophages / Q000502:physiology* / Q000637:transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera; D013539:Simian virus 40; D013491:Suppressor Factors, Immunologic / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D006649:Histocompatibility Antigens; D013491:Suppressor Factors, Immunologic; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin