title: Stored urine as a reservoir of gentamicin-resistant bacteria and as a site of conjugative R-plasmid transfer.
abstract: For some years we have observed the dissemination of gentamicin-resistant strains of Enterobacteriaceae in our hospital and we have reported the spread of antibiotic resistance by plasmid transfer. Stored urine was suspected to be a reservoir for gentamicin-resistant enterobacteria and to be a means of dissemination of gentamicin resistance amongst different species. We now report an epidemiological analysis based on biotyping of isolates and R-plasmid typing, and show that dissemination of bacterial strains and R-plasmids was not associated with disposable urine reservoirs, but dissemination of strains and plasmids was observed when non-disposable urine bottles were used in the wards.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagano
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
mesh_terms: D001431:Bacteriological Techniques; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000302:isolation & purification; D004812:Epidemiologic Methods; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D011815:R Factors; D013048:Specimen Handling; D014556:Urine / Q000382:microbiology*
chemical_list: D005839:Gentamicins