Enzymatic determination of acetate in serum.
With 100 microliters sample volume or less acetate can be measured accurately in serum without deproteinization by a coupled enzymatic reaction in a spectrophotometer. The reaction is linear from 250 to 5000 mumol/l acetate with a detection limit of 25 mumol/l. The imprecision from day to day was found to be 2.82% using frozen human pool serum with added sodium acetate. The method was found reliable in routine use, e.g. in man.
Bartelt
U
U
Kattermann
R
R
D000106:Acetate-CoA Ligase; D000085:Acetates / Q000097:blood*; D002950:Citrate (si)-Synthase; D006801:Humans; D007202:Indicators and Reagents; D008291:Malate Dehydrogenase; D008832:Microchemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000085:Acetates; D007202:Indicators and Reagents; D008291:Malate Dehydrogenase; D002950:Citrate (si)-Synthase; D000106:Acetate-CoA Ligase