[A method for inducing the point C----T transitions in DNA sequences corresponding to the ends of restriction sites].
A new method for inducing of C----T substitutions into cytosine-containing restriction sites is developed. The method, based on the selective modification of cytosine residues in DNA sticky ends by sodium bisulfite, was illustrated by induction of a base substitution (C----T at the BamHI site of pBR322 plasmid DNA.
Medvedev
O A
OA
Timchenko
T V
TV
Dianov
G L
GL
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes
Metod polucheniia tochkovykh C----T tranzitsiĭ po uchastkam DNK, sootvetstvuiushchim kontsam saĭtov restriktsii.