title: Cellular distribution of type I and type II receptors for transforming growth factor-beta.
abstract: Affinity labeling of target cells for transforming growth factor-beta (TGF beta) by cross-linking with 125I-TGF beta via disuccinimidyl suberate or by the photoreactive analogue 4-azidobenzoyl-125I-TGF beta has revealed the presence of multiple TGF beta receptor forms. Two distinct types of TGF beta receptors can be distinguished based on structural analysis of the 125I-TGF beta-labeled species by peptide mapping. Type I TGF beta receptors include the 280-kilodalton labeled receptor form previously found to be the subunit of a disulfide-linked TGF beta receptor complex. (Massagué, J. (1985) J. Biol. Chem. 260, 7059-7066), as well as a 65-kDa labeled receptor form present in all cell lines examined, and a 130-140-kDa labeled receptor form detected only in 3T3-L1 cells. The 280-kDa form is the major TGF beta receptor species in most cell lines examined, but is apparently absent in rat skeletal muscle myoblasts. Type I TGF beta receptors bind TGF beta with an apparent Kd of 50-500 pM. Type II TGF beta receptors include an 85-kDa labeled receptor form present in all mammalian cells examined and a 110-kDa labeled receptor form present in chick embryo fibroblasts. Type II TGF beta receptors bind TGF beta with an apparent Kd of about 50 pM. Except for the 280-kDa type I TGF beta receptor form, none of the TGF beta receptor forms described here is found as part of a disulfide-linked receptor complex. All the TGF beta receptor forms described here behave as intrinsic membrane proteins exposed on the surface of intact cells.
authors.forename: S
authors.initials: S
authors.lastname: Cheifetz
authors.forename: B
authors.initials: B
authors.lastname: Like
authors.forename: J
authors.initials: J
authors.lastname: Massagué
mesh_terms: D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D004220:Disulfides / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis; D010455:Peptides / Q000378:metabolism*; D010777:Photochemistry; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018125:Receptors, Transforming Growth Factor beta; D013388:Succinimides / Q000494:pharmacology; D014018:Tissue Distribution; D015291:Transforming Growth Factors
chemical_list: D000345:Affinity Labels; D004220:Disulfides; D010455:Peptides; D011956:Receptors, Cell Surface; D018125:Receptors, Transforming Growth Factor beta; D013388:Succinimides; D015291:Transforming Growth Factors; C019358:disuccinimidyl suberate