Biphasic response to retinoic acid dose in differentiation of F9 cells into primitive endoderm-like cells.
The differentiation of F9 cells, as monitored by the secretion of basement membrane proteins, was biphasic depending on the dose of retinoic acid (RA) used. Secretion of laminin A reached a plateau at about 10 nM and increased again at more than 100 mM RA. A similar biphasic response was observed in the secretion of tissue-type plasminogen activator as well. In the presence of RA alone, this biphasic pattern of differentiation was stable for a long period of time, but the addition of 1 mM dibutyryl cAMP changed it to monophasic after 12 days of exposure.
Miki
K
K
Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Japan.
Kitagawa
Y
Y
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D007797:Laminin / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*
D007797:Laminin; D014212:Tretinoin; D003994:Bucladesine; D010959:Tissue Plasminogen Activator