Determination of estradiol-17-sulfate in human urine by a direct radioimmunoassay: urinary levels throughout the menstrual cycle.
The measurement of urinary estradiol-17-sulfate concentration by direct radioimmunoassay was established. The urinary estradiol-17-sulfate levels measured by the radioimmunoassay correlated well with those determined by high-performance liquid chromatography equipped with electrochemical detector. Estradiol-17-sulfate concentrations in early morning urine of six healthy adult men was 8.2 +/- 2.0 ng/mL, or 5.7 +/- 1.8 ng/mg creatinine. The urinary levels in women throughout the menstrual cycle showed a characteristic three-peak excretion pattern: the first and the second peaks appeared just after and three days before the urinary LH peak, and the third peak appeared a few days before menstruation.
Watanabe
K
K
Hokkaido Institute of Pharmaceutical Sciences, Japan.
Takanashi
K
K
Yoshizawa
I
I
D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000652:urine; D004958:Estradiol / Q000031:analogs & derivatives* / Q000652:urine; D004966:Estrogens, Conjugated (USP) / Q000652:urine*; D005260:Female; D006801:Humans; D007986:Luteinizing Hormone / Q000652:urine; D008597:Menstrual Cycle; D011863:Radioimmunoassay
D004966:Estrogens, Conjugated (USP); C048615:estradiol 17-sulfate; D004958:Estradiol; D007986:Luteinizing Hormone; D003404:Creatinine