Relationship between sensitivity to 4'-(9-acridinylamino)methanesulfon-m-anisidide and DNA topoisomerase II in a cold-sensitive cell-cycle mutant of a murine mastocytoma cell line.
The cold-sensitive (proliferating at 39.5 degrees C, reversibly arrested in GI-phase at 33 degrees C) cell-cycle mutant 21-Fb of the murine mastocytoma cell line P815 was used to study the effect of amsacrine on non-cycling cells. The sensitivity of arrested 21-Fb cells decreased less than 2-fold in cell survival experiments when compared to proliferating cells. In contrast, DNA breakage and stimulation of protein-DNA complex formation in intact or lysed cells was reduced approx. 10-fold in arrested cells and DNA topoisomerase II activity in arrested cells was only 5% of the activity in proliferating cells. Thus, there was no correlation between cell survival and DNA damage or DNA topoisomerase II activity in drug-treated cells.
Schneider
E
E
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
Hutchins
A M
AM
Darkin
S J
SJ
Lawson
P A
PA
Ralph
R K
RK
D000677:Amsacrine / Q000494:pharmacology*; D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002470:Cell Survival / Q000187:drug effects; D003080:Cold Temperature; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D012515:Mast-Cell Sarcoma; D051379:Mice; D009154:Mutation; D011506:Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured
D011506:Proteins; D000677:Amsacrine; D004247:DNA; D004250:DNA Topoisomerases, Type II