Mesangial cells express PDGF mRNAs and proliferate in response to PDGF.
Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and is released and/or synthesized by platelets, macrophages, endothelial cells, and rat mesangial cells. In the present investigation, we found that human glomerular mesangial cells in culture release a PDGF-like protein which competes for 125I-PDGF binding to human foreskin fibroblasts and is mitogenic for these fibroblasts. The competing and to a lesser extent the mitogenic activities present in the conditioned medium are partially recognized by an anti-PDGF antibody. Northern blot analysis of poly(A)+ RNA from human mesangial cells demonstrates the expression of both PDGF A- and B-chain mRNAs. PDGF also binds to mesangial cells in a specific manner and stimulates DNA synthesis and cell proliferation. These data suggest that a PDGF-like protein secreted by mesangial cells or released from platelets, monocytes, or endothelial cells during glomerular inflammation may function as an autocrine or a paracrine growth factor for these cells. The biological role of PDGF in mediating proliferative and other inflammatory events in the glomerulus remains to be identified.
Shultz
P J
PJ
Department of Medicine, Veterans Administration Medical Center, Cleveland, Ohio.
DiCorleto
P E
PE
Silver
B J
BJ
Abboud
H E
HE
D002455:Cell Division; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D005920:Glomerular Mesangium / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D007231:Infant, Newborn; D007672:Kidney Cortex / Q000166:cytology*; D008297:Male; D008854:Microscopy, Electron; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D012867:Skin / Q000166:cytology; D014158:Transcription, Genetic
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor