Hydroxyl radical induced cross-linking between phenylalanine and 2-deoxyribose.
Hydroxy radicals induce cross-linking between phenylalanine (Phe) and 2-deoxyribose (dR) via formation of corresponding free radical intermediates. The cross-linked products were separated and identified by capillary gas chromatography-mass spectrometry. When phenylalanine and 2-deoxyribose radicals were generated in a 1:1 ratio, the predominant interaction was between Phe and dR radicals while the Phe-Phe and dR-dR cross-links were less abundant. The newly discovered cross-link between 2-deoxyribose and phenylalanine may serve as a model for radiation or free radical induced cross-linking between DNA and proteins and in general between sugar moieties and amino acids.
Farahani
M
M
Center for Radiation Research, National Bureau of Standards, Gaithersburg, Maryland 20899.
Simic
M G
MG
D003855:Deoxyribose / Q000528:radiation effects*; D005609:Free Radicals; D008401:Gas Chromatography-Mass Spectrometry; D006878:Hydroxides; D017665:Hydroxyl Radical; D013058:Mass Spectrometry; D010649:Phenylalanine / Q000528:radiation effects*
D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; D010649:Phenylalanine; D003855:Deoxyribose