title: Transforming genes in duck hepatic carcinoma--mht(raf) and Ha-ras.
abstract: The transfection of NIH 3T3 cells was performed with DNAs from 2 duck primary hepatic carcinomas (DHC 40K, 9K) and 1 tumor-adjacent liver tissue (TAL, 9N). Transfectants were found from 40K, 9K and 9N DNAs. The secondary transfectants were obtained after transfection of RAT-1 cells with DNAs from primary transfectants. After hybridization with Ha-ras, Ki-ras, N-ras and mht oncogenes, it was found that duck mht (5.2 and 3.2 kb EcoRI fragments) and duck Ha-ras (3.4 kb EcoRI fragments) were present in all these transformants. This is the first report on transforming genes in duck primary hepatic cancer as well as tumor-adjacent liver tissue.
authors.affiliation: Department of Biochemistry & Molecular Biology, Shanghai Cancer Institute.
authors.forename: H
authors.initials: H
authors.lastname: Tan
authors.forename: C T
authors.initials: CT
authors.lastname: Liu
authors.forename: W N
authors.initials: WN
authors.lastname: Hong
authors.forename: G Y
authors.initials: GY
authors.lastname: Tang
authors.forename: S Y
authors.initials: SY
authors.lastname: Zhang
authors.forename: Z X
authors.initials: ZX
authors.lastname: Lu
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Chen
authors.forename: H Q
authors.initials: HQ
authors.lastname: Jiang
authors.forename: J R
authors.initials: JR
authors.lastname: Gu
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000662:veterinary*; D004273:DNA, Neoplasm / Q000235:genetics; D004372:Ducks; D011905:Genes, ras; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics / Q000662:veterinary*; D051379:Mice; D009857:Oncogenes; D011201:Poultry Diseases / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
chemical_list: D004273:DNA, Neoplasm