[Cytotoxic and suppressor activity of human peripheral blood lymphocytes stimulated by interleukin-2 and phytohemagglutinin before and after fractionation in the percoll gradient].
The cultivation of peripheral blood lymphocytes (PBL) obtained from patients with colorectal or bladder carcinoma and melanoma and from healthy donors in the presence of interleukin-2 (IL-2) and PHA resulted in the induction of cytotoxic activity against autologous and/or allogeneic tumour cells in 12 out of 13 patients and in 10 out of 10 donors. A higher level of cytolytic activity was achieved when PBL were separated by means of Percoll density gradient (1.077; 1.067 and 1.056 g/ml) centrifugation and the cells of fraction II (1.077-1.067 g/ml) were employed in the experiment, the level of cytotoxicity being elevated in all cases (1.7-fold elevation in donors and 2-fold elevation in patients on the average). The addition of fraction I (1.067-1.056 g/ml) to fraction II prevented (PHA + IL-2)-mediated induction of cytotoxic activity in all the patients, but in 4 out of 10 donors, i.e. cells of fraction I expressed a suppressor activity. The immunofluorescent analysis has shown that fraction II was enriched by T cells (92%) and depleted of monocytes (7%), as compared to unseparated PBL (66% and 27%, respectively). On the contrary, fraction I was characterized by a decreased T cell ratio (36%) and an increased monocyte level (up to 69%).
Abronina
I F
IF
Malakhova
N V
NV
Figurin
K M
KM
Anan'ev
V S
VS
D002469:Cell Separation; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D009369:Neoplasms / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011205:Povidone; D012822:Silicon Dioxide; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology*
D007376:Interleukin-2; D010835:Phytohemagglutinins; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone
Tsitotoksicheskaia i supressornaia aktivnost' stimulirovannykh interleĭkinom-2 i fitogemaggliutininom limfotsitov perifericheskoĭ krovi cheloveka do i posle razdeleniia v gradiente perkolla.