Characterization of presynaptic beta-adrenoceptors facilitating endogenous noradrenaline release in the portal vein of permanently cannulated, freely moving rats.
We investigated the nature of presynaptic beta-adrenoceptors and the possible heterogeneity of these receptors in the portal vein nervous plexus of freely moving unanesthetized rats using the differential blockade technique with CGP 20712A as a highly beta 1-selective antagonist, ICI 118,551 as a very beta 2-selective antagonist and fenoterol and endogenous NA as beta 2- and beta 1-selective agonists respectively. The fenoterol (0.25 mg/kg)-induced increase of the basal NA level (290%) was dose dependently decreased by 0.1, 0.3 and 1.0 mg/kg ICI 118,551, but was not affected by a high dose (3.0 mg/kg) of CGP 20712A. During electrical stimulation (2 Hz, 3 ms, 5 mA) of the portal vein nervous plexus, 0.25 mg/kg fenoterol induced a 2.1-fold increase in NA overflow compared to the control stimulation value. ICI 118,551 was also able to decrease the fenoterol-induced enhancement during stimulation. During stimulation in the presence of CGP 20712A and fenoterol, the control stimulation value was not significantly decreased. Pretreatment with yohimbine (0.5 mg/kg) was used to create a strong beta 1-stimulus by raising the intra-synaptic NA level through blockade of the inhibitory alpha 2-adrenoceptors. Under these conditions, CGP 20712A did not deminish the yohimbine-induced enhancement of the stimulus evoked NA overflow, clearly indicating the absence of the beta 1-adrenoceptor subtype. ICI 118,551 (0.1 mg/kg) was also unable to influence the evoked NA overflow under these conditions, implying that, even at high concentrations, NA is not able to facilitate its own release by stimulation of the presynaptic beta 2-adrenoceptor.(ABSTRACT TRUNCATED AT 250 WORDS)
Remie
R
R
Department of Pharmacology and Therapeutics, University of Groningen, The Netherlands.
Knot
H J
HJ
Bos
E A
EA
Zaagsma
J
J
D000818:Animals; D002404:Catheterization; D004357:Drug Synergism; D005280:Fenoterol / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D008297:Male; D009638:Norepinephrine / Q000097:blood / Q000378:metabolism*; D011169:Portal Vein / Q000378:metabolism*; D011412:Propanolamines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000187:drug effects; D015016:Yohimbine / Q000494:pharmacology
D007093:Imidazoles; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; D005280:Fenoterol; D015016:Yohimbine; C026777:ICI 118551; C049763:CGP 20712A; D009638:Norepinephrine