Separation and characterization of metallothionein in two species of seals by high performance liquid chromatography-atomic absorption spectrophotometry.
Metallothionein (MT) was separated from the livers of two species of seals, ribbon seal (Histriophoca fasciata) and harbour seal (Phoca vitulina), by high performance liquid chromatography--atomic absorption spectrophotometry (HPLC-AAS). Both gel permeation and anion-exchange HPLC demonstrated that MT was present as a mixture of at least two isoforms in the livers of both species of seals.
Mochizuki
Y
Y
Suzuki
K T
KT
Sunaga
H
H
Kobayashi
T
T
Doi
R
R
D000818:Animals; D010872:Caniformia / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008099:Liver / Q000032:analysis*; D008668:Metallothionein / Q000302:isolation & purification*; D046048:Seals, Earless / Q000378:metabolism*; D013045:Species Specificity; D013054:Spectrophotometry, Atomic / Q000379:methods
D008668:Metallothionein