Allele replacement in Escherichia coli by use of a selectable marker for resistance to spectinomycin: replacement of the lexA gene.
We replaced the Escherichia coli lexA gene by a segment of DNA coding for resistance to spectinomycin and streptomycin. The use of this segment expands the range of selectable markers usable for allele replacement. The availability of this null lexA mutation will facilitate genetic analysis of lexA and the SOS regulon.
Hill
S A
SA
Department of Biochemistry and Molecular Biology, University of Arizona,Tucson 85721.
Little
J W
JW
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics*; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics*; D015183:Restriction Mapping; D013014:SOS Response, Genetics; D012697:Serine Endopeptidases; D000198:Spectinomycin / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics*; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D012097:Repressor Proteins; D012697:Serine Endopeptidases; D000198:Spectinomycin; D014157:Transcription Factors; D000099160:LexA Repressor Protein