Transformation/neodifferentiation of human skin fibroblasts by acute oncornaviruses and dexamethasone.
We demonstrated that the Kirsten murine sarcoma virus (KiMSV) and the Harvey murine sarcoma virus (HaMSV) converted human skin fibroblasts (HSF) into adipocytes. Adipocytic conversion of HSF by KiMSV and HaMSV was dependent on the presence of glucocorticosteroids. The Kirsten murine leukemia virus, the Harvey murine sarcoma [corrected] virus and the amphotropic helper virus (AP292) were ineffective by themselves. Balb murine sarcoma virus and Moloney murine sarcoma virus were, to a lesser degree, able to effect adipocytic conversion of HSF. In contrast, the feline sarcoma virus and the simian sarcoma virus did not cause this conversion. Together, the results suggest a role for certain oncogenes and glucocorticosteroids in the transformation/neodifferentiation of human cells.
Kopelovich
L
L
Laboratory of Cancer Genetics and Cancer Prevention, VA Medical Center, Bay Pines, Fla.
Fenyk
J
J
D001706:Biopsy; D002471:Cell Transformation, Neoplastic / Q000503:physiopathology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005347:Fibroblasts / Q000473:pathology / Q000503:physiopathology*; D011905:Genes, ras; D006801:Humans; D012190:Retroviridae / Q000502:physiology*; D009053:Sarcoma Viruses, Murine / Q000502:physiology; D012867:Skin / Q000473:pathology* / Q000503:physiopathology
D003907:Dexamethasone