title: In vitro expression of the alpha and beta subunits of the Na,K-ATPase.
abstract: Our analysis of the cloned alpha 3 protein strongly suggests that this c-DNA represents a bona fide Na+,K+ ATPase isoform. Its similarity to alpha + may have made its detection in tissues by gel migration or immunoreactivity difficult. Expression of an enzymatically active alpha 3 beta Na+K+ATPase either in a completely in vitro system or in a heterologous tissue culture system will clearly establish the biochemical properties of this isoform. Development of alpha 3 specific immunochemical probes will allow a proper assessment of its in vivo expression.
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven CT.
authors.forename: M
authors.initials: M
authors.lastname: Gilmore-Hebert
authors.forename: R W
authors.initials: RW
authors.lastname: Mercer
authors.forename: J W
authors.initials: JW
authors.lastname: Schneider
authors.forename: E J
authors.initials: EJ
authors.lastname: Benz
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D003001:Cloning, Molecular; D007527:Isoenzymes / Q000235:genetics; D046911:Macromolecular Substances; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D013698:Templates, Genetic
chemical_list: D007527:Isoenzymes; D046911:Macromolecular Substances; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase