Autoradiography of [3H]U-69593 binding sites in rat brain: evidence for kappa opioid receptor subtypes.
In vitro quantitative autoradiography was used to determine the distribution of [3H]U-69593 binding sites in rat brain. The highest density of binding was found in areas of the tel- and diencephalon while certain areas of the mes- and metencephalon were moderately labeled. The distribution of [3H]U-69593 binding sites corresponds closely (but not precisely) to the distribution of sites labeled by [3H]EKC and [3H]bremazocine at room temperature but differs substantially from the distribution of sites labeled by [3H]EKC at 4 degrees C (as described by others). These anatomical findings support previous biochemical evidence indicating that [3H]U-69593 selectively labels a kappa opioid receptor subtype with characteristics that differ from the kappa receptors labeled by [3H]EKC at 4 degrees C.
Nock
B
B
Department of Psychiatry, Washington University School of Medicine, St. Louis, MO 63110.
Rajpara
A
A
O'Connor
L H
LH
Cicero
T J
TJ
D000700:Analgesics / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D044482:Benzeneacetamides; D001575:Benzomorphans / Q000378:metabolism; D001665:Binding Sites; D001923:Brain Chemistry; D003496:Cyclazocine / Q000031:analogs & derivatives / Q000378:metabolism; D016314:Ethylketocyclazocine; D008297:Male; D011759:Pyrrolidines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000032:analysis*; D017473:Receptors, Opioid, kappa; D013696:Temperature
D000700:Analgesics; D044482:Benzeneacetamides; D001575:Benzomorphans; D011759:Pyrrolidines; D011957:Receptors, Opioid; D017473:Receptors, Opioid, kappa; D016314:Ethylketocyclazocine; C027248:bremazocine; C045444:U 69593; D003496:Cyclazocine