Induction of calcium influx in immature T lymphocytes by anti-FT-1 monoclonal antibody.
The functional role of FT-1 antigen was investigated by measuring changes in intracellular [Ca2+]i after the addition of anti-FT-1 monoclonal antibody to murine T leukemic cells and embryonic thymocytes. Anti-FT-1 induced a rapid and sustained increase in cytosolic free [Ca2+]i and this effect was completely blocked by EGTA and La3+. These results suggest that FT-1 molecule is functionally associated with a plasma membrane calcium ion channel in embryonic thymocytes.
Kasai
M
M
Department of Cellular Immunology, National Institute of Health, Tokyo, Japan.
Kataoka
T
T
Tokunaga
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation; D004533:Egtazic Acid / Q000494:pharmacology; D005333:Fetus / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D007811:Lanthanum / Q000494:pharmacology; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000941:Antigens; D015220:Calcium Channels; D004533:Egtazic Acid; D007811:Lanthanum; D002118:Calcium