A comparative study of the type-3 fimbriae of Klebsiella species.
Type-3 fimbriae isolated from members of five different species of Klebsiella were 4-5 nm in diameter and agglutinated the tannic acid-treated erythrocytes of ox and, in some cases, the untanned erythrocytes of fowl. In sodium dodecyl sulphate-polyacrylamide gel electrophoresis, the type-3 fimbrial proteins had mol. wts in the range 19 500-21 500. Hydrophobic amino acids comprised 39.6% of all the amino acids of the type-3 fimbrial protein of K. oxytoca strain 70/1. The type-1 fimbrial protein of Klebsiella had a mol. wt of c. 18 000 and the type-1 fimbriae were serologically distinct from the type-3 fimbriae. Our results for the type-3 fimbriae of Klebsiella were compared with those of others for morphologically similar and serologically related thin fimbriae of Salmonella and Yersinia.
Old
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D000596:Amino Acids / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D010861:Fimbriae, Bacterial / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000276:immunology; D007709:Klebsiella / Q000648:ultrastructure*; D007711:Klebsiella pneumoniae / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008970:Molecular Weight; D013045:Species Specificity
D000596:Amino Acids; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D006388:Hemagglutinins