The tumour promoter 12-O-tetradecanoylphorbol-13-acetate increases the activities of some peroxisome-associated enzymes in in vitro cell culture.
A study was conducted on the effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on peroxisomal enzyme activities in mouse embryo fibroblasts C3H/10T1/2 C18 cells and chemically transformed C3H/10T1/2 MCA16 cells. TPA is a potent tumour promoter and treatment with this compound of the two cell lines induced peroxisomal fatty acid beta-oxidation, carnitine acetyltransferase, palmitoyl-CoA hydrolase, and catalase activities after 240 h of treatment. Stimulation of the corresponding enzyme activities was dose-related and cycloheximide inhibited the TPA-induced enzyme activities, except that of carnitine acetyltransferase. The MCA16 cells appeared to be more sensitive than the C18 cells in inducing peroxisome-associated enzyme activities after TPA treatment. The activities of the microsomal marker, NADPH-cytochrome c reductase and the mitochondrial marker, glutamate dehydrogenase were not enhanced by TPA treatment. The results indicate that TPA has peroxisomal effects and may be classified as a peroxisome proliferator.
Lillehaug
J R
JR
Berge
R K
RK
D000818:Animals; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008830:Microbodies / Q000201:enzymology*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010704:Phorbols; D003513:Cycloheximide; D002374:Catalase; D005969:Glutamate Dehydrogenase; D009251:NADPH-Ferrihemoprotein Reductase; D002332:Carnitine O-Acetyltransferase; D010170:Palmitoyl-CoA Hydrolase; D013755:Tetradecanoylphorbol Acetate