title: The application of monoclonal antibody panels to characterize pestivirus isolates from ruminants in Great Britain.
abstract: Monoclonal antibodies were prepared against bovine virus diarrhoea virus and hog cholera virus. They were used to test 101 field isolates of ruminant pestivirus in a simple binding assay using an indirect immunoperoxidase label on fixed cell cultures. The monoclonals were divided into three panels: (1) pestivirus group specific, (2) hog cholera specific, (3) selectively reactive with ruminant pestiviruses. The reaction patterns with panel 3 were analyzed by a computer spreadsheet to determine the percentage match with seven reference patterns. Field viruses could be divided into two main groups. Group A consisted of 73 (87%) of the 84 bovine isolates and three (19%) of the 16 ovine, and was reactive with 40% or more of panel 3. Group B showed only limited reactivity with panel 3 and comprised 13/16 (81%) of the ovine (border disease) isolates together with the remaining 11 (13%) bovine viruses.
authors.affiliation: Central Veterinary Laboratory, Weybridge, Surrey, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Edwards
authors.forename: J J
authors.initials: JJ
authors.lastname: Sands
authors.forename: J W
authors.initials: JW
authors.lastname: Harkness
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000940:Antigenic Variation; D006692:Classical Swine Fever Virus / Q000276:immunology; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology; D001330:Electronic Data Processing; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D010576:Pestivirus / Q000276:immunology*; D012016:Reference Values; D012418:Ruminants / Q000382:microbiology*; D006113:United Kingdom; D014776:Virus Cultivation
chemical_list: D000911:Antibodies, Monoclonal