title: beta-Adrenergic agonist activity of a monoclonal anti-idiotypic antibody.
abstract: Hybridoma cells bearing monoclonal antibody against the beta-adrenergic ligand alprenolol were used as an immunogen to raise monoclonal anti-idiotypic antibodies. Of six anti-idiotypic antibodies, which inhibit ligand binding, three were able to recognize beta-adrenergic receptors. One of them, mAb2B4, an IgM that could be amplified into ascites, binds to the beta-adrenergic catecholamine receptors of intact epidermoid A431 cells and precipitates receptors solubilized from plasma membranes by digitonin. This antibody identifies the beta 2-adrenergic receptor of A431 cells as a single 55-kDa protein and stimulates adenylate cyclase activity. This stimulation is inhibited by the beta-adrenergic antagonist propranolol.
authors.forename: J G
authors.initials: JG
authors.lastname: Guillet
authors.forename: S V
authors.initials: SV
authors.lastname: Kaveri
authors.forename: O
authors.initials: O
authors.lastname: Durieu
authors.forename: C
authors.initials: C
authors.lastname: Delavier
authors.forename: J
authors.initials: J
authors.lastname: Hoebeke
authors.forename: A D
authors.initials: AD
authors.lastname: Strosberg
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000276:immunology*; D000526:Alprenolol / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002478:Cells, Cultured; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D011943:Receptors, Adrenergic, beta / Q000276:immunology
chemical_list: D000318:Adrenergic beta-Agonists; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D011943:Receptors, Adrenergic, beta; D000526:Alprenolol; D000262:Adenylyl Cyclases