Insertion of Mu into the Shrunken 1 gene of maize affects transcriptional and post-transcriptional regulation of Sh1 RNA.
Insertion of a Mu transposable element at the Shrunken 1 (Sh1) locus of maize has resulted in kernels with the typical collapsed appearance of sh mutants. Molecular analysis of the mutant gene has revealed the presence of a 1.4 kb insertion immediately upstream from the normal transcriptional start site. Mu insertion has brought about a series of changes in gene expression: the mRNA cap site has been shifted downstream so that it now lies inside the Mu element; transcription is reduced approximately sixfold, and the sh mRNA steady-state level is less than 4% of that found in the nonmutant. This disparity reflects a mutational defect in post-transcriptional regulation which is manifested as a decrease in Sh RNA abundance.
Ortiz
D F
DF
Department of Genetics, University of Georgia, Athens 30602.
Rowland
L J
LJ
Gregerson
R G
RG
Strommer
J N
JN
D001483:Base Sequence; D015152:Blotting, Northern; D004251:DNA Transposable Elements; D004720:Endonucleases; D005786:Gene Expression Regulation; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000096:biosynthesis*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases