Stainer and Scholte's pertussis medium with an alternative buffer.
A comparative study of the Stainer and Scholte chemically defined growth medium (SS) for Bordetella pertussis, and a modification of it (MSS), was made. The Tris buffer which is reported to have adverse effects was replaced in MSS with sodium glycerophosphate. By using generation time as a measure of the growth promoting properties of the media, we found that the MSS was superior to SS for B. pertussis strain CN5612/67, which was used as a vaccine strain in Norway. Additionally by employing the 'dilution to extinction' method we showed that MSS supported B. pertussis growth from smaller inocula than the original SS medium. No significant differences in the quality of vaccines made from organisms grown in the two alternative media were observed in animal tests for potency and safety. The difference in buffers in SS and MSS had no influence on the formation of the agglutinogens, as tested by agglutination with specific factor sera. It was confirmed that liquid media gave a better total expression of the agglutinogens than solid media.
Lothe
R A
RA
Frøholm
L O
LO
Westre
G
G
Kjennerud
U
U
D000371:Agglutination; D000818:Animals; D001886:Bordetella pertussis / Q000254:growth & development* / Q000276:immunology; D002021:Buffers; D003470:Culture Media; D051379:Mice; D010567:Pertussis Vaccine / Q000276:immunology; D011817:Rabbits; D013997:Time Factors
D002021:Buffers; D003470:Culture Media; D010567:Pertussis Vaccine