Improved method for azole antifungal susceptibility testing.
A reproducible method is described for the determination of the MICs of ketoconazole, miconazole, fluconazole, and itraconazole with sharp endpoints when employed with either yeasts or molds. A semisolid medium is used with controlled pH and standardized inoculum. The time of reading results is a critical factor in the conduct of this test. The medium is simple to prepare and has a relatively long refrigerator shelf life in a user-ready state, requiring only the addition of a freshly prepared inoculum after restoration to room temperature.
Gordon
M A
MA
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
Lapa
E W
EW
Passero
P G
PG
D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D015725:Fluconazole; D005658:Fungi / Q000187:drug effects*; D006801:Humans; D017964:Itraconazole; D007654:Ketoconazole / Q000031:analogs & derivatives / Q000494:pharmacology; D008825:Miconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D014230:Triazoles / Q000494:pharmacology
D000935:Antifungal Agents; D001393:Azoles; D014230:Triazoles; D017964:Itraconazole; D008825:Miconazole; D015725:Fluconazole; D007654:Ketoconazole