title: Chromosome-encoded beta-lactamases of Citrobacter diversus. Interaction with beta-iodopenicillanate and labelling of the active site.
abstract: Both forms of the chromosome-encoded beta-lactamase of Citrobacter diversus react with beta-iodopenicillanate at a rate characteristic of class A beta-lactamases. The active site of form I was labelled with the same reagent. The sequence of the peptide obtained after trypsin hydrolysis is identical with that of a peptide obtained in a similar manner from the chromosome-encoded beta-lactamase of Klebsiella pneumoniae.
authors.affiliation: Università degli Studi dell'Aquila Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Amicosante
authors.forename: A
authors.initials: A
authors.lastname: Oratore
authors.forename: B
authors.initials: B
authors.lastname: Joris
authors.forename: M
authors.initials: M
authors.lastname: Galleni
authors.forename: J M
authors.initials: JM
authors.lastname: Frère
authors.forename: J
authors.initials: J
authors.lastname: Van Beeumen
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002876:Chromosomes, Bacterial / Q000201:enzymology*; D002954:Citrobacter / Q000201:enzymology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D010397:Penicillanic Acid / Q000494:pharmacology / Q000031:analogs & derivatives; D010446:Peptide Fragments / Q000032:analysis; D065093:beta-Lactamase Inhibitors
chemical_list: D007527:Isoenzymes; D010446:Peptide Fragments; D065093:beta-Lactamase Inhibitors; D010397:Penicillanic Acid; C031528:6-iodopenicillanic acid