The subpopulation of brain coated vesicles that carries synaptic vesicle proteins contains two unique polypeptides.
Coated vesicles have been purified in the past on the basis of their remarkably homogeneous structure, not their function. We have succeeded in isolating two subpopulations of bovine brain coated vesicles that carry specific "cargoes," in this case two synaptic vesicle membrane polypeptides (Mr = 95,000 and 65,000). Monoclonal antibodies that recognize cytoplasmic domains of these polypeptides can penetrate the clathrin coat and recognize them on the outer surface of the coated vesicle membrane. An immunoadsorption technique could therefore be used to fractionate coated vesicles on the basis of their membrane composition. The subpopulations have the normal complement of conventional coated vesicle proteins. Exclusive, however, to the subpopulations that carry synaptic vesicle polypeptides are two new coated vesicle polypeptides (Mr = 38,000 and 29,000).
Pfeffer
S R
SR
Kelly
R B
RB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001666:Binding Sites, Antibody; D001692:Biological Transport; D001921:Brain / Q000648:ultrastructure*; D002417:Cattle; D002458:Cell Fractionation; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D011992:Endosomes / Q000378:metabolism*; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins