title: Effects of acute stress, (1-24)ACTH administration and changes in superfusion temperature and flow rate on the in vitro secretion of glucocorticosteroids and aldosterone from the Mongolian gerbil (Meriones unguiculatus) adrenal gland.
abstract: The release of glucocorticosteroids and aldosterone rapidly decreased after start of superfusion and reached a steady base-line within 60-90 min of superfusion. While secretion markedly varied between experiments, it was very constant in the same experiment (coefficient of variation: 7.4-2.2% for glucocorticosteroids and 5.8-3.9% for aldosterone). After repeated exposure of adrenal tissue to 1 IU/ml (1-24)ACTH, glucocorticosteroid release progressively increased; under the same conditions aldosterone secretion was not changed. Glucocorticosteroid secretion from glands of animals stressed by 1-hr confinement or of animals injected with 6 IU (1-24)ACTH was significantly higher than that of controls over the 60-min superfusion period. Aldosterone secretion was not affected significantly by these pretreatments. After reduction of temperature from 35 to 1 degrees C, steroid release ceased. Elevation of temperature from 12 to 32 degrees C resulted in a linear increase of glucocorticosteroid and aldosterone secretion. A highly significant positive correlation was found between glucocorticosteroid and aldosterone amounts secreted from adrenals superfused at temperatures between 1 and 35 degrees C (r = 0.91, n = 116, P less than 0.0001). Changes of flow rate from 0.5 to 1.5 ml/min for 5 min induced a short term (1 min) stimulation of glucocorticosteroid and aldosterone release; reduction of flow rate to 0.5 ml/min for 5 min drastically diminished secretion of steroids below control levels for 1 min.
authors.forename: M
authors.initials: M
authors.lastname: Fenske
mesh_terms: D000208:Acute Disease; D000302:Adrenal Cortex / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives*; D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D003366:Cosyntropin / Q000494:pharmacology*; D005849:Gerbillinae / Q000378:metabolism*; D005938:Glucocorticoids / Q000378:metabolism*; D066298:In Vitro Techniques; D013312:Stress, Physiological / Q000378:metabolism*; D013696:Temperature
chemical_list: D005938:Glucocorticoids; D003366:Cosyntropin; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone