Isolation and characterization of chicken liver lysosomes.
The distribution patterns of chicken liver lysosomal enzymes were studied in iso-osmotic gradients of Percoll. The lysosomal enzymes separated by Percoll gradients showed three different types of distribution. In contrast with rat liver lysosomes, purified chicken liver lysosomes were very stable during storage at 4 degrees C.
Nakabayashi
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T
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Ikezawa
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H
D000118:Acetylglucosaminidase / Q000302:isolation & purification; D000818:Animals; D001192:Arylsulfatases / Q000302:isolation & purification; D002374:Catalase / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D002645:Chickens; D005952:Glucose-6-Phosphatase / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification; D051381:Rats
D002374:Catalase; D009247:NADH, NADPH Oxidoreductases; C028796:iodophenyl-nitrophenyl-phenyltetrazolium reductase; D005952:Glucose-6-Phosphatase; D001192:Arylsulfatases; D000118:Acetylglucosaminidase