Formation of reactive oxygen species and of 8-hydroxy-2'-deoxyguanosine in DNA in vitro with betel-quid ingredients.
Using a chemiluminescence technique, superoxide anion (O2-.) and H2O2 were shown to be formed in vitro, above pH 9.5, from betel-quid (BQ) ingredients, such as areca-nut extract and catechu. The formation of O2-. was enhanced by Fe2+, Fe3+ and Cu2+ and inhibited by Mn2+. Saliva was found to inhibit both O2-. and H2O2 formation from BQ ingredients. Upon incubation of DNA at alkaline pH with areca-nut extract or catechu, in the presence or absence of Fe3+, 8-hydroxy-2'-deoxyguanosine was formed, as quantified by high-performance liquid chromatography. The data suggest a possible role of reactive oxygen species (ROS) in the etiology of oral cancer in betel quid chewers.
Friesen
M
M
International Agency for Research on Cancer, Lyon, France.
Maru
G
G
Bussachini
V
V
Bartsch
H
H
Nair
U
U
Nair
J
J
Floyd
R A
RA
D000080242:8-Hydroxy-2'-Deoxyguanosine; D001114:Areca; D004247:DNA / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements; D010936:Plant Extracts / Q000378:metabolism; D010946:Plants, Medicinal; D013481:Superoxides / Q000378:metabolism
D010936:Plant Extracts; D013481:Superoxides; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D006861:Hydrogen Peroxide; D003849:Deoxyguanosine