Use of enzyme label for quantitative evaluation of liposome adhesion on cell surface: studies with J774 macrophage monolayers.
A method for quantitation of cell surface-bound liposomes utilizing J774 macrophage monolayers is developed. Surface-bound biotinyl-containing and 125I-labeled liposomes were quantified with avidin-peroxidase in an ELISA-like assay. Peroxidase substrate absorbance values were recalculated into the absolute amount of liposomal lipid using a special calibration plot. Total liposome uptake by macrophages was determined following the binding of 125I radioactivity. The approach suggested allows quantitative evaluation of the changes in the content of surface-adhered liposomes during their interaction with cells in vitro.
Trubetskoy
V S
VS
Institute of Experimental Cardiology, USSR Cardiology Research Center, Moscow.
Dormeneva
E V
EV
Tsibulsky
V P
VP
Repin
V S
VS
Torchilin
V P
VP
D000327:Adsorption; D001360:Avidin; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D008081:Liposomes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D009195:Peroxidase; D010587:Phagocytosis
D008081:Liposomes; D001360:Avidin; D009195:Peroxidase