[Avian CELO adenovirus as a possible contaminant in cell cultures of Japanese quail embryos].
Avian adenovirus CELO was found to replicate poorly in Japanese quail embryos (JQE) and cell cultures of them. The infectious process in these systems was latent. The antigen of adenovirus CELO in JQE cell culture was detectable by the fluorescent antibody method (FAM) within the first 24-72 hours after inoculation as fluorescent cytoplasmic granules. Subsequently, fluorescence of nuclei and macrophage cytoplasm was observed. The results indicate that JQE and their cell cultures are not contaminated with avian adenovirus CELO despite regular circulation of this agent among avian populations. The advantages of FAM (rapidity and clearness) for identification of adenovirus as substrates contaminant as compared with other biological methods have been demonstrated.
Burgasova
M P
MP
Sorokina
T B
TB
Chaplygina
N M
NM
Bogomolova
N N
NN
D000256:Adenoviridae / Q000502:physiology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000259:Aviadenovirus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D003370:Coturnix / Q000382:microbiology*; D004625:Embryo, Nonmammalian; D005455:Fluorescent Antibody Technique; D011784:Quail / Q000382:microbiology*; D013997:Time Factors; D014776:Virus Cultivation; D014779:Virus Replication
D000914:Antibodies, Viral
Adenovirus ptits CELO kak vozmozhnyĭ kontaminant kul'tur kletok émbrionov iaponskikh perepelov.