Immunogenicity in mice of the sequential polypeptides (Ala-Tyr-Glu-Gly)n, (Ala-Glu-Tyr-Gly)n, (Glu-Ala-Tyr-Gly)n and (Glu-Tyr-Ala-Gly)n and their linkage with the major histocompatibility complex.
The immunogenicity of the four sequential polymers of alpha-L-amino acids referred to below was studied in inbred strains of mice. The responses were linked to the H-2 haplotype, and the Ir genes controlling the responses were mapped to the IA region. The responses were restricted to two haplotypes as follows: (Ala-Tyr-Glu-Gly)n and (Ala-Glu-Tyr-Gly)n - H-2k; (Glu-Ala-Tyr-Gly)n - H-2b; (Glu-Tyr-Ala-Gly)n - H-2k and b. The absence of reciprocal crossreactions among all of these polymers, plus the sequential polymers (Tyr-Ala-Glu-Gly)n and (Tyr-Glu-Ala-Gly)n at both the antibody and T-cell levels, indicated that each of these six polymers was indeed structurally unique.
Maurer
P H
PH
Department of Biochemistry, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa.
Lai
C H
CH
Zeiger
A R
AR
Turchin
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D008040:Genetic Linkage; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D010455:Peptides; D011948:Receptors, Antigen, T-Cell; C064214:poly(glutamyl-tyrosyl-alanyl-glycine); C064217:poly(alanyl-glutamyl-tyrosyl-glycine); C064215:poly(alanyl-tyrosyl-glutamyl-glycine); C064216:poly(glutamyl-alanyl-tyrosyl-glycine)