Effects of benzyl alcohol on enzyme activities and D-glucose transport in kidney brush-border membranes.
Addition of increasing amounts of benzyl alcohol progressively reduced the steady-state anisotropies of diphenylhexatriene and trimethylammoniumdiphenylhexatriene in brush-border membranes from rat kidney. The decrease in order of membrane lipids, equivalent for 50 mM benzyl alcohol to that produced by a rise in temperature of approx. 6 degrees C, had no effect on the activities of alkaline phosphatase or gamma-glutamyltranspeptidase. On the other hand, benzyl alcohol markedly inhibited the D-glucose uptakes measured in the presence of a 100 mM sodium gradient. For concentrations less than 30 mM, benzyl alcohol reduced the Jmax without significant effects on Km, 22Na+ uptake or the vesicular volume of brush-border preparations. Comparable results were obtained substituting octanol for benzyl alcohol. Our data strongly suggest that, at constant temperature, the D-glucose carrier present in renal brush-border membranes is extremely sensitive to variations in membrane physical state.
Carrière
B
B
Le Grimellec
C
C
D020003:1-Octanol; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000494:pharmacology*; D001593:Benzyl Compounds / Q000494:pharmacology*; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D007700:Kinetics; D008297:Male; D008433:Mathematics; D008566:Membranes / Q000187:drug effects / Q000201:enzymology; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D000442:Octanols / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D001592:Benzyl Alcohols; D001593:Benzyl Compounds; D009004:Monosaccharide Transport Proteins; D000442:Octanols; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; D012964:Sodium; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D005947:Glucose; D019905:Benzyl Alcohol; D020003:1-Octanol