title: On the mechanism of the chromate reduction by glutathione: ESR evidence for the glutathionyl radical and an isolable Cr(V) intermediate.
abstract: With a view of elucidating the role of glutathione (GSH) in the biochemical pathways of the chromate-exposure related carcinogenesis, we carried out electron spin resonance (ESR) spectroscopic investigations of the chromate-GSH redox reactions. The ESR measurements, employing spin-traps, provide evidence for the involvement of the glutathione (GS) radical, as well as an isolable Cr(V)-glutathione intermediate. These results indicate a new mechanism for the reduction of chromate by GSH in in vitro cellular environment and help understand the (unexpected) increase in Cr(VI)-induced DNA strand breaks at elevated GSH levels.
authors.affiliation: Department of Chemistry, West Virginia University, Morgantown 26506.
authors.forename: X L
authors.initials: XL
authors.lastname: Shi
authors.forename: N S
authors.initials: NS
authors.lastname: Dalal
mesh_terms: D002840:Chromates; D004355:Drug Stability; D004578:Electron Spin Resonance Spectroscopy; D005978:Glutathione / Q000031:analogs & derivatives; D019803:Glutathione Disulfide; D010084:Oxidation-Reduction
chemical_list: D002840:Chromates; D005978:Glutathione; D019803:Glutathione Disulfide