The role of amino acid catabolism in the formation of the tricarboxylic acid cycle intermediates and ammonia in anoxic rat heart.
Amino acid catabolism, the tricarboxylic acid cycle intermediates and ammonia formation were studied in isolated perfused rat heart under anoxia. The total net anaplerosis due to amino acid degradation in anoxia was equal to that in oxygenation (6.29 and 6.09 mumol/g dry weight per h, respectively) as a result of the increased transamination of glutamic and aspartic acids. During anoxic perfusion, the rate of catabolism of glutamic and aspartic acids was 1.5-times higher than in normoxia, while depletion of branched-chain amino acids, lysine, proline, arginine and methionine, was inhibited. Alanine was the product of excessive degradation of glutamic and aspartic acids. Under anaerobic conditions, in spite of inhibition of amino acid deamination, ammonia formation was increased 2.7-fold as compared to oxygenation. The principal amount of ammonia (96%) was produced at degradation of adenine nucleotides. A 2.5-fold increase in the pool of the tricarboxylic acid cycle intermediates under anoxia was associated mainly with accumulation of succinate. The data suggest that the coupling of alanine- and aspartate amino transferases is a mechanism controlling the tricarboxylic acid cycle pool size in anoxic heart.
Pisarenko
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Solomatina
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Studneva
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D000227:Adenine Nucleotides / Q000378:metabolism; D000332:Aerobiosis; D000596:Amino Acids / Q000502:physiology*; D000641:Ammonia / Q000096:biosynthesis*; D000693:Anaerobiosis; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002952:Citric Acid Cycle; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000227:Adenine Nucleotides; D000596:Amino Acids; D005971:Glutamates; D009124:Muscle Proteins; D001224:Aspartic Acid; D018698:Glutamic Acid; D000641:Ammonia; D010100:Oxygen