A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium.
A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
Michiels
T
T
Université Catholique de Louvain, Unité de Microbiologie, Brussels, Belgium.
Popoff
M Y
MY
Durviaux
S
S
Coynault
C
C
Cornelis
G
G
D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D010957:Plasmids; D012093:Replicon; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics* / Q000472:pathogenicity
D004251:DNA Transposable Elements