Pertussis toxin selectively abolishes hormone induced lowering of cytosolic calcium in GH3 cells.
Pertussis toxin, PT, abolishes inhibitory regulation of adenylate cyclase by cell surface receptors. Inhibitors of adenylate cyclase in GH3 cells, namely somatostatin and the muscarinic cholinergic agonist carbachol, lower the cytosolic free Ca2+ concentration. [Ca2+]i and cause hyperpolarization. These responses are selectively abolished by PT. It is concluded that the effects of somatostatin and carbachol to lower [Ca2+]i and to hyperpolarize are secondary to their inhibitory action on adenylate cyclase. In contrast, PT does not impair the TRH induced rise in [Ca2+]i in GH3 cells demonstrating that the coupling of TRH receptors to Ca2+ mobilization is not mediated by a PT substrate.
Schlegel
W
W
Wuarin
F
F
Zbaren
C
C
Wollheim
C B
CB
Zahnd
G R
GR
D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism*; D037342:Pertussis Toxin; D010911:Pituitary Neoplasms / Q000378:metabolism*; D051381:Rats; D013004:Somatostatin / Q000494:pharmacology*; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology; D010566:Virulence Factors, Bordetella
D037361:Adenylate Cyclase Toxin; D001427:Bacterial Toxins; D010566:Virulence Factors, Bordetella; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D013973:Thyrotropin-Releasing Hormone; D002217:Carbachol; D037342:Pertussis Toxin; D000262:Adenylyl Cyclases; D002118:Calcium