[UHF-dielectrometry in the study of parameters of native adenylate cyclase complex during modulation of its activity].
Some features of molecular mechanisms of adenylate-cyclase system of native human erythrocytes were studied by the UHF-dielectrometry method. The results obtained show great changes in cell structure hydration when there is some influence on the membrane protein components which are involved in transmembrane signal transformation. The observed effects permit definite conclusions to be drawn concerning some molecular mechanisms of cell regulation. The obtained data demonstrated the following: the key role of N-protein, spatial separation of PGE2 regulatory centres and hormones on the beta-receptor, system modulation by the change of Ca2+ ions concentration in the presence of PGE2 and the connection of the alpha-receptor with the calcium slow channel.
Malaia
L T
LT
Shchegoleva
T Iu
TIu
Bakhova
L K
LK
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D015232:Dinoprostone / Q000494:pharmacology; D004553:Electric Conductivity; D004789:Enzyme Activation; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D011846:Radio Waves; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000502:physiology; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000502:physiology
D000067956:Adenylyl Cyclase Inhibitors; D015220:Calcium Channels; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D015232:Dinoprostone
Issledovanie metodom SVCh-diélektrometrii parametrov nativnogo adenilattsiklaznogo kompleksa pri moduliatsii ego aktivnosti.