Specific determination of arylsulfatase A activity.
Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was quantitatively determined using HPLC equipped with an amperometric detector. The method was used to analyze the activity of AS-A in biological materials.
Inoue
H
H
Seyama
Y
Y
Yamashita
S
S
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001921:Brain / Q000201:enzymology*; D002553:Cerebroside-Sulfatase / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D051381:Rats; D013429:Sulfatases / Q000378:metabolism*
C001956:ascorbic acid 2-sulfate; D013429:Sulfatases; D002553:Cerebroside-Sulfatase; D001205:Ascorbic Acid