Monoclonal antibody to enkephalins with binding characteristics similar to opiate receptor.
Monoclonal antibodies to enkephalins were established by immunization of mice with met-enkephalin, leu-enkephalin or both. Twenty-three clones with a high titer were classified into 6 types according to the binding properties to enkephalins and their derivatives. Antibody LM 239 showed binding characteristics similar to opiate receptor. It has a very high affinity to enkephalins and their derivatives which have a potent opioid activity, but a low affinity to enkephalin derivatives which devoid of opioid activity. The binding of 3H-met-enkephalin to the antibody was inhibited by naloxone and morphine, although the ID50 values were considerably higher than the Ka values of the alkaloids to opiate receptor.
Deguchi
T
T
Yokoyama
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004723:Endorphins / Q000276:immunology; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000276:immunology; D016308:Enkephalin, Leucine-2-Alanine; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives / Q000276:immunology; D004745:Enkephalins / Q000276:immunology*; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D009270:Naloxone / Q000494:pharmacology; D011957:Receptors, Opioid / Q000378:metabolism*; D018822:alpha-Endorphin
D000911:Antibodies, Monoclonal; D004723:Endorphins; D004745:Enkephalins; D011957:Receptors, Opioid; D009270:Naloxone; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; C022118:enkephalinamide-Met, Ala(2)-; D018822:alpha-Endorphin; D016308:Enkephalin, Leucine-2-Alanine; D009020:Morphine