alpha-Bungarotoxin and P15 toxin binding site in mammalian sympathetic ganglia.
alpha-Bungarotoxin (alpha-Bgtx) demonstrates a specific and saturable binding to rat superior cervical ganglion, yet it does not block ganglionic response mediated by nicotinic receptors in the guinea pig vas deferens-hypogastric nerve preparation. P15 toxin, a probe for ganglionic nicotinic receptor, prevents the binding of alpha-Bgtx to rat ganglia, and alpha-Bgtx prevents the ganglioplegic action of hexamethonium. Hexamethonium does not block the binding of alpha-Bgtx and P15 to rat ganglia. It is concluded that alpha-Bgtx and P15 bind to ganglionic nicotinic receptor at a common site which is different from that of cholinergic agonists and antagonists.
Gotti
C
C
Spagnoli
D
D
Omini
C
C
Clementi
F
F
D000818:Animals; D001665:Binding Sites; D002038:Bungarotoxins / Q000378:metabolism* / Q000494:pharmacology; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D006168:Guinea Pigs; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000037:antagonists & inhibitors; D007001:Hypogastric Plexus / Q000187:drug effects; D008297:Male; D010791:Phrenic Nerve / Q000187:drug effects; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism; D013045:Species Specificity; D009435:Synaptic Transmission / Q000187:drug effects; D014649:Vas Deferens / Q000294:innervation
D002038:Bungarotoxins; D006584:Hexamethonium Compounds; D011950:Receptors, Cholinergic; D018738:Hexamethonium