Some physico-chemical and biological properties of egg-Sendai virus with altered haemagglutinin-neuraminidase protein.
Some physico-chemical and biological properties of egg-cultivated Sendai (egg-Sendai) virus particles with altered haemagglutinin-neuraminidase (HN) protein were studied. These particles were obtained accidentally from purified egg-Sendai virus stored at +4 degrees C. Egg-Sendai with altered HN protein was not infectious for cultured cells, but could still infect embryonated eggs. Though egg-Sendai virus with altered HN protein and glycoproteins had no haemagglutination activity, it unexpectedly possessed a relatively high neuraminidase (NA) activity representing about 70% of the activity of normal particles. Since polyacrylamide gel and rocket immunoelectrophoresis indicated that the altered particles contained only F protein, possibly the native HN was converted into a molecule of the same molecular mass as F protein.
Popa
L M
LM
Repanovici
R
R
Iliescu
R
R
Mihalache
O
O
Cajal
N
N
D000956:Antigens, Viral / Q000032:analysis; D015707:HN Protein; D006387:Hemagglutination, Viral; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000201:enzymology / Q000276:immunology / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology*; D014760:Viral Fusion Proteins; D014771:Virion / Q000201:enzymology / Q000502:physiology*
D000956:Antigens, Viral; D015707:HN Protein; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D009439:Neuraminidase