Comparison between brain paste and polymer [125I]standards for quantitative receptor autoradiography.
[125I]standards prepared from grey and white matter from rat brain have been compared to commercially available polymer-based standards using two types of radiation-sensitive film for use in quantitative receptor autoradiography. There was a linear relationship between the natural log of optical density and radioactivity (dpm/mm2) for both types of tissue standard exposed to Hyperfilm [3H] or betamax from 1-14 days. No difference between either the slopes or intercepts of these lines for each period of exposure was identified. There was also no significant difference between the slopes and intercepts of tissue-based standards and the lowest 5 activity levels of polymer standards. Over the complete range of ten radioactivity levels of polymer standards, the linear relationship was lost and a quadratic function was the best model for describing the relationship between 1n optical density and radioactivity. Moreover, a linear relationship between section thickness over the range frequently used in receptor studies (5-20 microns) was observed. These results suggest that the lower activity levels of polymer standards are comparable to tissue standards and can be used for quantitative receptor autoradiography.
Davenport
A P
AP
Parke-Davis Research Unit, Addenbrooke's Hospital Site, Cambridge, U.K.
Hall
M D
MD
D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry; D007457:Iodine Radioisotopes / Q000032:analysis; D008297:Male; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis*
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface