Rat kidney brush border enzyme activity following subchronic oral lead exposure.
Rats received 0.1% lead acetate in their drinking water for 3 weeks or for 6 weeks, at which time renal brush border fractions were obtained for measurement of enzyme activity. Renal brush border preparations from Pb2+-exposed rats exhibited statistically significant decreases in the activity of gamma-glutamyl transpeptidase and alanine aminopeptidase after 3 or 6 weeks of treatment. There was an increase in the activity of alkaline phosphatase which was statistically significant after 3 weeks of Pb2+ exposure. The (Na+,K+) adenosine triphosphatase activity and urokinase activity, located in the basolateral membrane fractions, were unchanged by Pb2+ exposure, as were the protein and phospholipid contents of the brush border fractions. The results are compared to those following acute exposure to Pb2+ or Cd2+.
Teichert-Kuliszewska
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Nicholls
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D000284:Administration, Oral; D000469:Alkaline Phosphatase / Q000378:metabolism; D000626:Aminopeptidases / Q000037:antagonists & inhibitors; D000818:Animals; D018826:CD13 Antigens; D007668:Kidney / Q000201:enzymology*; D007854:Lead / Q000378:metabolism; D007855:Lead Poisoning / Q000201:enzymology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008871:Microvilli / Q000201:enzymology; D009942:Organometallic Compounds; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000037:antagonists & inhibitors
D008565:Membrane Proteins; D009942:Organometallic Compounds; D010743:Phospholipids; D007854:Lead; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D000626:Aminopeptidases; D018826:CD13 Antigens; D014568:Urokinase-Type Plasminogen Activator; C008261:lead acetate