Use of streptavidin to detect biotin-containing proteins in plants.
A procedure to detect biotinyl proteins after fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was developed. Proteins were immobilized on nitrocellulose and biotin-containing proteins were detected by probing with 125I-streptavidin. Using this procedure a small survey of biotinyl protein in plants was undertaken. In total four biotin-containing proteins were detected in higher plants of molecular weights 62,000, 50,000, 34,000, and 31,000. These biotinyl proteins were not ubiquitous in the plants surveyed. In the cyanobacterium Anabeana variabilis, a single biotin-containing protein of 21,000 Da was detected. In isolated spinach chloroplasts, the two biotinyl proteins detected were soluble. The results are discussed in relation to acetyl-CoA carboxylase.
Nikolau
B J
BJ
Wurtele
E S
ES
Stumpf
P K
PK
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D001426:Bacterial Proteins; D001710:Biotin / Q000302:isolation & purification*; D002736:Chloroplasts / Q000032:analysis; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D010936:Plant Extracts / Q000032:analysis; D010940:Plant Proteins / Q000302:isolation & purification*; D019809:Streptavidin
D001426:Bacterial Proteins; D010936:Plant Extracts; D010940:Plant Proteins; D001710:Biotin; D003101:Collodion; D019809:Streptavidin; D000103:Acetyl-CoA Carboxylase