title: Calcium-dependence of somatostatin binding to receptors.
abstract: Binding of 125I-[Tyr11] somatostatin (S14) and 125I-[Tyr1]S14 has been studied in pancreatic acini and cerebral cortex. Ca2+-dependence of somatostatin binding to receptors was observed only with the highly non degradable iodinated analog 125I-[Tyr11] somatostatin but not with 125I-[Tyr1] somatostatin. The inhibitory activity of S14 on secretin-stimulated cAMP cellular content was decreased when Ca2+ concentration in the medium was reduced to 30 nM, indicating that the Ca2+ dependence of somatostatin binding to receptors is also present with the native peptide.
authors.forename: C
authors.initials: C
authors.lastname: Susini
authors.forename: J P
authors.initials: JP
authors.lastname: Esteve
authors.forename: N
authors.initials: N
authors.lastname: Vaysse
authors.forename: A
authors.initials: A
authors.lastname: Ribet
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D010179:Pancreas / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017481:Receptors, Somatostatin; D013004:Somatostatin / Q000378:metabolism*
chemical_list: D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D017481:Receptors, Somatostatin; D013004:Somatostatin; D000242:Cyclic AMP; D002118:Calcium