title: Oxidative metabolism of propylthiouracil by peroxidases from rat bone marrow.
abstract: 1. Propylthiouracil (PTU) was degraded by myeloperoxidase (MPO) or eosinophil peroxidase (EPO), purified from rat bone marrow, in the presence of H2O2 and Cl-. In the absence of either H2O2 or Cl-, MPO and EPO do not degrade PTU. Optimum concentrations of KCl for MPO and EPO were 50 and 250 mM, respectively. 2. The characteristics of PTU degradation by MPO-H2O2-Cl- were similar to those of the chlorinating activity of the peroxidase. 3. Hypochlorous acid as well as MPO-H2O2-Cl- also degraded PTU. Metabolites of PTU degradation by MPO-H2O2-Cl-, which were separated by C18 reversed phase h.p.l.c., were the same as those produced by hypochlorous acid. 4. Of the metabolites of PTU formed by MPO-H2O2-Cl-, one was identified as PTU sulphonic acid (6-propyl-4-hydroxypyrimidine-2-sulphonate) and another seemed to be propyluracil.
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Miki
authors.forename: M
authors.initials: M
authors.lastname: Hosokawa
authors.forename: H
authors.initials: H
authors.lastname: Sayo
authors.forename: K
authors.initials: K
authors.lastname: Kariya
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000201:enzymology* / Q000378:metabolism; D047209:Eosinophil Peroxidase; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000302:isolation & purification*; D010544:Peroxidases / Q000302:isolation & purification*; D011189:Potassium Chloride / Q000494:pharmacology; D011441:Propylthiouracil / Q000493:pharmacokinetics*; D051381:Rats; D012973:Sodium Hypochlorite / Q000494:pharmacology
chemical_list: D011189:Potassium Chloride; D011441:Propylthiouracil; D012973:Sodium Hypochlorite; D047209:Eosinophil Peroxidase; D010544:Peroxidases; D009195:Peroxidase