Reduced insulin binding to human fat cells following beta-adrenergic stimulation--experimental evidence and studies in patients with a phaeochromocytoma.
The effect of beta-adrenergic stimulation on insulin binding was studied in human fat cells in vitro. Isoproterenol rapidly (approximately 5 min) reduced insulin binding through a beta-adrenergic and dose-dependent mechanism. The reduced binding was enhanced by the addition of adenosine deaminase and was also elicited by the addition of dibutyryl cAMP. This effect was due to a decreased number of binding sites. The reduction was rapidly reversed by propranolol (t1/2 approximately 10 min) and other beta-adrenoreceptor blocking agents. Insulin binding was also measured in fat cells from 6 patients with a phaeochromocytoma. A significant negative correlation between tracer binding and the log value of total urinary catecholamine excretion was found (r = -0.821, p less than 0.05). Mean tracer insulin binding was reduced about 30% as compared to cells from 16 carefully matched control subjects. Decreased insulin binding was again mainly attributable to a decreased number of binding sites. Thus, beta-adrenergic stimulation, both in vitro and in vivo, leads to a decreased number of binding sites for insulin in human fat cells.
Lönnroth
P
P
Wesslau
C
C
Stenström
G
G
Tisell
L E
LE
Smith
U
U
D000273:Adipose Tissue / Q000378:metabolism*; D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000328:Adult; D000368:Aged; D002395:Catecholamines / Q000652:urine; D005260:Female; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010673:Pheochromocytoma / Q000378:metabolism*; D011190:Potassium Cyanide / Q000494:pharmacology; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism*
D000319:Adrenergic beta-Antagonists; D002395:Catecholamines; D007328:Insulin; C008147:insulin, iodo-; D011972:Receptor, Insulin; D007545:Isoproterenol; D011190:Potassium Cyanide