Structure of the K74 antigen from Escherichia coli O44:K74:H18, a capsular polysaccharide containing furanosidic beta-KDO residues.
The structure of the capsular K74 antigen of E. coli H702c (O44:K74:H18) was elucidated by determination of the composition, 1H- and 13C-n.m.r. and c.d. spectroscopy, periodate oxidation, and methylation analysis of the polysaccharide and of a trisaccharide obtained by mild acid hydrolysis. The K74 antigen has the repeating unit----3)-beta-D-Ribf-(1----2)-beta-D-Ribf-(1----6)-beta-++ +KDOf-(2----. Of the repeating units, approximately 65% are O-acetylated, most probably at C-2 of the 3-linked ribose.
Ahrens
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R
Max-Planck-Institut für Immunbiologie, Freiburg, F.R.G.
Jann
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B
Jann
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K
Brade
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H
D000107:Acetylation; D000942:Antigens, Bacterial / Q000032:analysis*; D016667:Bacterial Capsules; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007163:Immunosorbent Techniques; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012266:Ribose; D013400:Sugar Acids / Q000032:analysis*; D014312:Trisaccharides / Q000032:analysis
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; D014312:Trisaccharides; C058138:capsular polysaccharide K74; D010504:Periodic Acid; C002532:2-keto-3-deoxyoctonate; D012266:Ribose; C009288:metaperiodate