Stage-dependent restoration of sensory dorsal columns following spinal cord transection in anuran tadpoles.
In frogs sensory axons from the lumbar dorsal roots ascend in the dorsal column of the spinal cord to terminate in the medulla and cerebellum. The response of these axons to complete transection of the thoracic spinal cord has been analysed in Rana temporaria tadpoles at different stages of development. The presence and position of dorsal column axons were assessed by using the anterograde transport of horseradish peroxidase or by electrophysiological methods. Before developmental stage VIII, dorsal column axons can grow across the transection and reach their normal areas of termination in the brainstem. Axons that do cross the transection follow their normal pathways. From stage VIII onwards this capacity for growth is largely lost. These results are discussed in terms of the relation between neurogenesis, axon growth and axonal regeneration.
Clarke
J D
JD
Tonge
D A
DA
Holder
N H
NH
D000200:Action Potentials; D000818:Animals; D001369:Axons / Q000502:physiology*; D002452:Cell Count; D003714:Denervation; D004558:Electric Stimulation; D005727:Ganglia, Spinal / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology; D007814:Larva / Q000254:growth & development / Q000502:physiology; D008675:Metamorphosis, Biological; D009416:Nerve Regeneration; D011896:Rana temporaria; D013997:Time Factors