Tn4201, a beta-lactamase transposon in Staphylococcus aureus.
We report the preliminary characterization of a 6.7-kilobase transposon, Tn4201, encoding resistance to penicillin associated with the conjugative plasmid pCRG1600 in Staphylococcus aureus. Tn4201 is capable of inverting in orientation (frequently observed in derivatives of pCRG1600) and is expressed equally well in either orientation. The element is also capable of movement to alternative plasmid and chromosomal sites. Tn4201 exhibits rec-independent transposition in an apparent site-specific manner and may play an important role in the resistance of staphylococci to beta-lactam compounds.
Weber
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Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178.
Goering
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D000900:Anti-Bacterial Agents / Q000494:pharmacology; D007446:Chromosome Inversion; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D010403:Penicillin Resistance / Q000235:genetics; D011815:R Factors; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D014161:Transduction, Genetic; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D047090:beta-Lactams; D004262:DNA Restriction Enzymes; D001618:beta-Lactamases