title: Analysis of splenic lymphocytes with high electrophoretic mobility in adult and aged nude mice.
abstract: Electrophoretic mobility (EPM) and surface markers of splenic lymphocytes in adult (8 weeks old) and aged (over 1 year old) nude mice were investigated. Splenic lymphocytes in nude mice showed a bimodal pattern consisting of low mobility lymphocytes (LML) corresponding to B cells and high mobility lymphocytes (HML). The HML of nude mice showed the following immunological characteristics: (1) surface Ig- cells; (2) asialo GM1+ cells; (3) an increase in natural killer (NK) activity after depletion of B cells; (4) abrogation of the HML peak and NK activity after treatment with anti-asialo GM1 and complement. These findings suggested that HML in nude mice were NK cells. The mobility of NK cells was slightly lower than that of T cells in normal mice, although their histograms greatly overlapped each other. In the spleen cells of nude mice, there was a significant increase in the numbers of Thy-1+ cells and a decrease in the intensity of asialo GM1 antigen as a function of age. The surface markers of HML were Thy-1+- asialo GM1++ in adult nude mice, but were Thy-1+ asialo GM1+ in aged nude mice. However, although HML in aged nude mice became Thy-1+, these had almost the same EPM as those in adult nude mice.
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: A
authors.initials: A
authors.lastname: Matsuzawa
authors.forename: T
authors.initials: T
authors.lastname: Iwaguchi
mesh_terms: D000367:Age Factors; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000032:analysis; D003165:Complement System Proteins / Q000276:immunology; D004586:Electrophoresis; D005455:Fluorescent Antibody Technique; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000032:analysis; D007694:Killer Cells, Natural / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis / Q000145:classification*; D051379:Mice; D008819:Mice, Nude / Q000276:immunology*; D013154:Spleen / Q000166:cytology*; D018800:Thy-1 Antigens
chemical_list: D000954:Antigens, Surface; D006028:Glycosphingolipids; D018800:Thy-1 Antigens; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D003165:Complement System Proteins