Quantitative analyses of the uncoupling activity of substituted phenols with mitochondria from flight muscles of house flies.
Uncoupling activity with flight-muscle mitochondria from house flies was measured for a series of weakly acidic uncouplers (substituted phenols) and compared with the protonophoric potency across lecithin liposomal membranes. The activity was linearly related to the protonophoric potency when such factors as the stability of anionic species in the membrane phase and the difference in the pH conditions of the extramembranous aqueous phase were taken into account. Relationships of the flight-muscle activity with activities measured previously with rat-liver mitochondria and spinach chloroplasts were linear. Our findings were further evidence for the shuttle-type mechanism of the uncoupling action of weakly acidic uncouplers.
Miyoshi
H
H
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Japan.
Fujita
T
T
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005426:Flight, Animal; D006793:Houseflies / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D015394:Molecular Structure; D009132:Muscles / Q000378:metabolism* / Q000648:ultrastructure; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology*; D011522:Protons; D051381:Rats; D012044:Regression Analysis; D013329:Structure-Activity Relationship; D014475:Uncoupling Agents
D010636:Phenols; D011522:Protons; D014475:Uncoupling Agents; D000255:Adenosine Triphosphate