Ouabain sensitivity of a chimeric alpha subunit (Torpedo/rat) of the (Na,K)ATPase expressed in Xenopus oocyte.
A cDNA for a chimeric alpha subunit of the (Na,K)ATPase was constructed and expressed in Xenopus oocytes in order to elucidate structural features involved in ouabain sensitivity. A chimeric alpha subunit, in which the N-terminal 165 amino acid sequence of ouabain-resistant rat alpha subunit, including the first two transmembrane segments (M1 and M2), was replaced by a sequence from the corresponding region of ouabain-sensitive Torpedo alpha subunit, was ouabain-sensitive, suggesting that the M1-M2 junction is a site responsible for ouabain sensitivity of the (Na,K)ATPase.
Noguchi
S
S
Department of Biology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Ohta
T
T
Takeda
K
K
Ohtsubo
M
M
Kawamura
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002678:Chimera / Q000187:drug effects*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D014101:Torpedo; D014981:Xenopus
D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase