A novel B cell line established from Ki-1-positive diffuse large cell lymphoma.
A novel cell line, designated KIS-1, was established from a patient with Ki-1-positive diffuse large cell lymphoma. Multiple phenotypic analysis of the KIS-1 cells was carried out with a total of 22 monoclonal antibodies defining hematopoietic cell subsets and lineages. The KIS-1 cells were positive for Ki-1, B4, HLA-DR, and 2D1 (common leucocyte) antigens, but were negative for the antigens reportedly specific for T cells, natural killer cells, granulocytes, monocytes, interdigitating reticulum cells and dendritic reticulum cells. The genomic analysis of the KIS-1 cells showed not only the rearrangement of JH and J kappa genes but also the probable rearrangement of C lambda genes. Moreover, the cells produced immunoglobulin lambda chains. Thus, KIS-1 was considered to be of B-cell lineage. The lymphoma-cell derivation of KIS-1 was based on the following facts. The cytochemical, immunologic, cytogenetic properties and the results of the molecular genomic analysis in the KIS-1 cells were essentially the same as those of the original tumor cells, and the KIS-1 cells were negative for Epstein-Barr virus-associated nuclear antigen. KIS-1 is the only known B-cell line derived from Ki-1-positive diffuse large cell lymphoma, and should be useful for defining the biological implications of Ki-1 antigen.
Kamesaki
H
H
Department of Internal Medicine, Faculty of Medicine, Kyoto University.
Miwa
H
H
Ohno
Y
Y
Miyanishi
S
S
Yamabe
H
H
Doi
S
S
Arita
Y
Y
Ohno
H
H
Tatsumi
E
E
Nishikori
M
M
D000943:Antigens, Differentiation / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes; D002869:Chromosome Aberrations; D019309:Epstein-Barr Virus Nuclear Antigens; D005803:Genes, Immunoglobulin; D006801:Humans; D007150:Immunohistochemistry; D017730:Ki-1 Antigen; D008223:Lymphoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D017730:Ki-1 Antigen