Effects of calcium entry blockers on calcium fluxes in a vascular smooth muscle cell line.
A smooth muscle cell line was used for studies on the activity of the voltage-sensitive calcium channel (VSCC). Upon exposure to a depolarizing solution or to a calcium channel activator, an increase in 45Ca2+ uptake was observed. Calcium entry blockers inhibited the uptake with potencies very similar to those observed in relaxing depolarization-induced constriction of aortic rings. The two enantiomers of the dihydropyridine PN 200-110 showed about a 300-fold potency difference in blocking 45Ca2+ uptake.
Rüegg
U T
UT
Cardiovascular Unit, Preclinical Research, Sandoz Inc., Basle, Switzerland.
Doyle
V M
VM
Hof
R P
RP
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002460:Cell Line; D004594:Electrophysiology; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D051381:Rats
D002121:Calcium Channel Blockers; D002118:Calcium