Molecular biological characterization of equine herpesvirus type 1 (EHV-1) isolates from ruminant hosts.
Isolates of equine herpesvirus type 1 (EHV-1) originating from affected antelope and cattle were compared with reference EHV-1 and EHV-4 isolates and were characterized. Based on cross-neutralization, DNA restriction profiles and blot-hybridization data these isolates could be characterized as EHV-1. One isolate (from an antelope) with a different restriction profile showed significant DNA homology with EHV-1, partial homology with EHV-4, and little or no homology with EHV-2 and HSV-1 DNAs. Blot hybridization revealed differences in DNA restriction fragments located at the termini of two isolates and size heterogeneity in the unique long/internal repeat junction fragment (UL/IR) of one isolate.
Chowdhury
S I
SI
Institute for Virology, Free University of Berlin, F.R.G.
Ludwig
H
H
Buhk
H J
HJ
D000818:Animals; D000866:Antelopes / Q000382:microbiology*; D001188:Artiodactyla / Q000382:microbiology*; D002417:Cattle / Q000382:microbiology*; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D006564:Herpesviridae / Q000302:isolation & purification*; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000302:isolation & purification*; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D015342:DNA Probes; D004279:DNA, Viral