Competition between DNA templates injected into Xenopus laevis oocytes.
We have examined the competition between microinjected DNA templates for endogenous Xenopus oocyte transcription factors. The results suggest that (a) coinjected templates compete with one another for transcription factors, (b) the competitive ability of a template is not necessarily a function of the rate of transcription of the gene, (c) competition is due to the presence of specific DNA sequences, and (d) preincubation of the test gene affects competition.
Mancebo
M J
MJ
Department of Molecular Genetics, University of Texas Systems Cancer Center, M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Etkin
L D
LD
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D005260:Female; D006657:Histones / Q000235:genetics; D008845:Microinjections; D009865:Oocytes; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis; D013698:Templates, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014982:Xenopus laevis
D006657:Histones; D012333:RNA, Messenger; D014157:Transcription Factors; D004247:DNA; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes