Determination of equilibrium binding affinity of distamycin and netropsin to the synthetic deoxyoligonucleotide sequence d(GGTATACC)2 by quantitative DNase I footprinting.
A new method for determining the equilibrium binding constant of antitumor drugs to specific DNA sequences by quantitative DNase I footprinting is presented. The use of a short synthetic DNA oligomer to define a homogeneous population of DNA binding sites enables the calculation of the free drug concentration and the fraction of DNA sites complexed with drug in solution and is described for the first time. Since a 1:1 stoichiometry is observed for each drug-oligomer DNA complex, it becomes possible to calculate equilibrium binding constants in solution. By use of this technique, the binding affinities of the nonintercalating drugs netropsin and distamycin to the synthetic oligonucleotide d(GGTATACC)2 are determined to be Ka (25 degrees C) = 1.0 X 10(5) and 2.0 X 10(5) M-1, respectively. Quantitation of the temperature dependence associated with complex formation results in a determination of standard enthalpies of -3.75 and -8.48 kcal mol-1 for the binding of netropsin and distamycin, respectively. Calculation of other thermodynamic parameters are found to be in agreement with previous studies and indicate that the DNA binding process for these compounds is predominantly enthalpy driven. This method of quantitative DNase I footprinting is demonstrated to be a useful technique for the measurement of drug affinities to specific binding sites on DNA oligomers which are designed and synthesized expressly for this purpose. Applications of the technique to the determination of drug binding affinities at specific sites within native DNA sequences are discussed.
Fish
E L
EL
Molecular Therapeutics, Inc., West Haven, Connecticut 06516.
Lane
M J
MJ
Vournakis
J N
JN
D001665:Binding Sites; D004247:DNA; D003850:Deoxyribonuclease I; D004214:Distamycins; D006146:Guanidines; D007700:Kinetics; D009429:Netropsin; D009838:Oligodeoxyribonucleotides; D011758:Pyrroles; D013816:Thermodynamics
D004214:Distamycins; D006146:Guanidines; D009838:Oligodeoxyribonucleotides; D011758:Pyrroles; D009429:Netropsin; C057781:d(GGTATACC); D004247:DNA; D003850:Deoxyribonuclease I