Murine intestinal intraepithelial lymphocytes I. Relationship of a novel Thy-1-,Lyt-1-,Lyt-2+, granulated subpopulation to natural killer cells and mast cells.
Highly purified populations of intraepithelial lymphocytes (IEL) were obtained from the murine small intestine. We found that 84% of IEL expressed Lyt-2 and that 45-55% possessed the unique phenotype, Thy-1-,Lyt-1-,Lyt-2+. Sixty percent of IEL had granules in their cytoplasm and thus resembled the large granulated lymphocytes associated with natural killer (NK) activity. However, less than 15% of IEL had NK activity in a 6-h assay. This activity resided in a subpopulation of Lyt-2- lymphocytes, leaving a large population of Thy-1-,Lyt-1-,Lyt-2+ granulated cells (45-55% of IEL) with unknown function. Sensitive radioenzymic assays for histamine showed that IEL from healthy CBA mice do not contain this amine. IgE-binding assays revealed that IEL, unlike mast cells, do not carry high-affinity receptors for IgE. Thus, only a small percentage of granulated IEL (less than 15%) possess NK activity, whereas 45-55% of IEL have granules, lack typical characteristics of mast cells, express the unique antigenic phenotype, Thy-1-,Lyt-1-,Lyt-2+ and have unknown function.
Petit
A
A
Ernst
P B
PB
Befus
A D
AD
Clark
D A
DA
Rosenthal
K L
KL
Ishizaka
T
T
Bienenstock
J
J
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D004847:Epithelial Cells; D005260:Female; D005434:Flow Cytometry; D006632:Histamine / Q000032:analysis; D007422:Intestines / Q000166:cytology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008214:Lymphocytes / Q000032:analysis / Q000145:classification*; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D011961:Receptors, Fc / Q000032:analysis; D018800:Thy-1 Antigens
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D011961:Receptors, Fc; D018800:Thy-1 Antigens; D006632:Histamine