Pulsatile release of growth hormone and prolactin from the primate pituitary in vitro.
Perifused anterior hemipituitaries from one male and 4 female monkeys released GH and PRL in a pulsatile pattern, with mean +/- SE interpulse intervals of 8.2 +/- 0.4 and 8.5 +/- 0.3 min, as determined by a cycle detection computer algorithm. Mean hormone concentrations in the perifusate fractions collected at 2-min intervals were 435 +/- 89 (GH) and 515 +/- 262 (PRL) ng/ml. Pulse amplitudes averaged 74 +/- 16 ng/ml for GH and 189 +/- 89 ng/ml for PRL. These findings suggest the presence of a high frequency pulsatile secretory mechanism within the primate pituitary.
Stewart
J K
JK
Clifton
D K
DK
Koerker
D J
DJ
Rogol
A D
AD
Jaffe
T
T
Goodner
C J
CJ
D000818:Animals; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D066298:In Vitro Techniques; D008254:Macaca nemestrina; D008297:Male; D010477:Perfusion; D010507:Periodicity; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D013004:Somatostatin / Q000494:pharmacology
D013004:Somatostatin; D011388:Prolactin; D013006:Growth Hormone