Inhibition of delayed hypersensitivity reactions by a new agent, cis-1-methyl-4-isohexylcyclohexane carboxylic acid (IG-10).
A newly synthesized compound, cis-1-methyl-4-isohexylcyclohexane carboxylic acid (IG-10), has been reported as an inhibitor of delayed hypersensitivity reaction. In the present paper, the mechanisms regarding the inhibitory action of IG-10 was investigated on delayed hypersensitivity reactions. p-Phenylenediamine-induced contact dermatitis in guinea pigs was significantly inhibited when the drug was given in a dose of 100 mg/kg p.o. at various times after challenge with the antigen. IG-10 inhibited both contact dermatitis and monocytes or neutrophils infiltrations induced by picryl chloride in mice. A skin reaction, similar to that seen in the case of delayed hypersensitivity reaction, was induced by an intradermal injection of phytohemagglutinin-P (PHA-P) stimulated lymphocytes in guinea pigs. This reaction was a useful method for assessing the effect of drugs on the release of lymphokines, particularly skin reactive factor (SRF). IG-10 in a concentration of 10(-5) g/ml inhibited the release of SRF as well as the release of migration inhibitory factor (MIF) from guinea pig lymphocytes stimulated by PHA-P. The reduction of delta 4-3-ketone of aldosterone and/or hydrocortisone by rat liver homogenates was not affected with IG-10, unlike that seen in the case of glycyrrhizin.
Koda
A
A
Nakatomi
I
I
Nakamura
K
K
Inoue
H
H
Kamimura
T
T
D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D003877:Dermatitis, Contact / Q000517:prevention & control; D004890:Erythema / Q000139:chemically induced; D006168:Guinea Pigs; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006854:Hydrocortisone / Q000378:metabolism; D006968:Hypersensitivity, Delayed / Q000517:prevention & control*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000378:metabolism; D008263:Macrophage Migration-Inhibitory Factors / Q000378:metabolism; D008297:Male; D051379:Mice
D003509:Cyclohexanecarboxylic Acids; D006634:Histamine H1 Antagonists; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D000450:Aldosterone; C045466:IG 10; D006854:Hydrocortisone