title: Two specific topoisomerase II inhibitors prevent replication of human cytomegalovirus DNA: an implied role in replication of the viral genome.
abstract: In this study, we show that human cytomegalovirus DNA synthesis is inhibited in infected confluent human embryonic lung cells treated with the DNA-intercalative topoisomerase II inhibitor 4-9'-(acridinylamino)methanesulfon-m-anisidide (m-AMSA). Similar inhibitory effects were observed with VM-26, a nonintercalative topoisomerase II inhibitor. This antiviral effect is not attributable to cytotoxic effects per se. Furthermore, m-AMSA appears to have a notably irreversible inhibitory effect on human cytomegalovirus DNA replication. No inhibition of viral DNA synthesis was observed with o-AMSA, a DNA-intercalative isomer of m-AMSA that does not inhibit topoisomerase II.
authors.affiliation: Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27599.
authors.forename: J D
authors.initials: JD
authors.lastname: Benson
authors.forename: E S
authors.initials: ES
authors.lastname: Huang
mesh_terms: D000677:Amsacrine / Q000031:analogs & derivatives / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000096:biosynthesis; D006801:Humans; D013713:Teniposide / Q000494:pharmacology; D059005:Topoisomerase II Inhibitors; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D004279:DNA, Viral; D059005:Topoisomerase II Inhibitors; D000677:Amsacrine; C033073:4'-(9-acridinylamino)methanesulfon-o-anisidide; D013713:Teniposide