Detection of a tumour-associated ganglioside in plasma of patients with neuroblastoma.
An abnormal circulating ganglioside was found in patients with neuroblastoma. This ganglioside appeared as a single band by resorcinol-HCl staining of thin-layer chromatograms of purified total gangliosides isolated from as little as 1 ml of patient plasma. It is a major ganglioside of neuroblastoma tumour tissue and was present (250-1500 pmol lipid-bound sialic acid/ml) in the plasma of five patients with widespread neuroblastoma. In contrast, the ganglioside was not detected (less than 50 pmol/ml) in plasma samples of six patients in complete remission, nor in plasma samples of seventeen healthy children and adults. Measurement of this circulating tumour-associated ganglioside should be clinically useful in neuroblastoma, offering a new approach to the detection of tumour and the evaluation of therapy.
Ladisch
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Wu
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D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002855:Chromatography, Thin Layer; D005260:Female; D005677:G(M1) Ganglioside / Q000097:blood; D005679:G(M3) Ganglioside / Q000097:blood; D005732:Gangliosides / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009447:Neuroblastoma / Q000097:blood* / Q000175:diagnosis
D005679:G(M3) Ganglioside; D005732:Gangliosides; C021298:ganglioside, GD1a; C033851:ganglioside, GD1b; D005677:G(M1) Ganglioside; C016481:trisialoganglioside GT1