Absence of alternative splicing in bcr-abl mRNA in chronic myeloid leukemia cell lines.
The major consequence of the Philadelphia (Ph) translocation in chronic myeloid leukemia (CML) is the formation of a bcr-abl hybrid oncogene encoding a tumor cell-specific protein P210bcr-abl. In contrast to this, in Ph chromosome-positive acute lymphoblastic leukemia (Ph + ALL), a P190bcr-abl can be observed. This P190bcr-abl has been implicated in acute rather than chronic leukemogenesis. Therefore, it can be hypothesized that the transition from chronic to blast phase in CML is accompanied by an alternative splice in the bcr-abl mRNA, which results in a switch of the production of P210bcr-abl into P190bcr-abl. Initial S1 nuclease protection mapping supported this theory. However, this result appears to be based on an artifact in the S1 analysis. By using the polymerase chain reaction we provide evidence for the absence of alternative splicing in bcr-abl mRNA in two CML blast crisis cell lines.
Hermans
A
A
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Selleri
L
L
Gow
J
J
Grosveld
G C
GC
D001752:Blast Crisis / Q000235:genetics* / Q000473:pathology; D002460:Cell Line; D015342:DNA Probes; D004720:Endonucleases; D005189:False Positive Reactions; D016044:Fusion Proteins, bcr-abl; D005784:Gene Amplification; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D015336:Molecular Probe Techniques; D009363:Neoplasm Proteins / Q000235:genetics*; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases
D015342:DNA Probes; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016044:Fusion Proteins, bcr-abl; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases