title: Role of Ca2+ and Na+ on luteinizing hormone release from the calf pituitary.
abstract: It has been proposed that gonadotropin-releasing hormone (GnRH) stimulates Ca2+ entry by activation of voltage-independent, receptor-mediated Ca2+ channels in the rat gonadotroph. Little work has been done on the role of calcium in GnRH-induced luteinizing hormone (LH) release in species other than the rat. Therefore, this study was done to compare the effects of agents that alter Ca2+ or Na+ entry on LH release from calf anterior pituitary primary cells in culture. GnRH (100 ng/ml), Ca2+ ionophore A23187 (2.5 microM), and the depolarizing agent ouabain (0.1-10 microM) all produced significant increases (P less than 0.05) in LH release; these effects were significantly reduced when the cells were preincubated with the organic Ca2+ channel blockers nifedipine (1-10 microM) and verapamil (1-10 microM) and with Co2+ (0.01-1 mM). The effect of ouabain was inhibited by tetrodotoxin (TTX; 1-10 nM) as well as by nifedipine at 0.1-10 microM. In contrast to its effect on rat pituitary LH release, TTX significantly inhibited GnRH-stimulated LH release at 1-100 nM. These results suggest that GnRH-induced LH release may employ Ca2+ as a second messenger in bovine gonadotrophs and support recent speculation that GnRH-induced Ca2+ mobilization may in part be voltage dependent.
authors.affiliation: Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A&M University, College Station 77843.
authors.forename: J P
authors.initials: JP
authors.lastname: Kile
authors.forename: M S
authors.initials: MS
authors.lastname: Amoss
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D003035:Cobalt / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D007986:Luteinizing Hormone / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D009543:Nifedipine / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D000001:Calcimycin; D003035:Cobalt; D013779:Tetrodotoxin; D010042:Ouabain; D007986:Luteinizing Hormone; D012964:Sodium; D014700:Verapamil; D008274:Magnesium; D009543:Nifedipine; D002118:Calcium