title: An enterokinase in the gut of pharate adult of Glossina morsitans morsitans Westwood (Diptera: Glossinidae).
abstract: An enterokinase (Enteropeptidase, EC. 3.4.21.9) has been described in the pharate adult of Glossina mositans morsitans. The enzyme is present in pharate adults, 21 days after pupation. It activated commercial crystalline bovine trypsinogen to trypsin. It showed affinity for concanavalin A bound to sepharose and was reversibly sensitive to boiling at pH 6.0. The apparent molecular weight, as determined by gel permeation on sepharose 6B-CL, suggests self-aggregation or an association with a large molecule (M.Wt. approximately equal to 2.5 X 10(6)).
authors.forename: R M
authors.initials: RM
authors.lastname: Vundla
authors.forename: D L
authors.initials: DL
authors.lastname: Whitehead
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D010450:Endopeptidases / Q000302:isolation & purification*; D004765:Enteropeptidase / Q000302:isolation & purification* / Q000378:metabolism; D004789:Enzyme Activation; D008970:Molecular Weight; D011679:Pupa / Q000201:enzymology; D014357:Trypsin / Q000378:metabolism; D014362:Trypsinogen / Q000378:metabolism; D014370:Tsetse Flies / Q000201:enzymology* / Q000254:growth & development
chemical_list: D014362:Trypsinogen; D010450:Endopeptidases; D014357:Trypsin; D004765:Enteropeptidase