title: Inhibitory action of somatostatin on meiotic maturation of cultured porcine follicular ova.
abstract: To investigate the physiological importance of somatotrophin release-inhibiting factor (SRIF), effects in vitro of synthetic SRIF 14 on germinal vesicle breakdown (GVB) of cultured porcine follicular ova were studied. The proportion of ova with GVB decreased gradually and significantly with increasing concentrations of SRIF 14 in the range from 6 X 10(-12) to 6 X 10(-7) M during a 22 h period of culture. The inhibitory effect was apparent for the period between 14 and 22 h in the course of culture but was reversed by a concomitant addition of anti-SRIF to the medium. Neither synthetic oxytocin, vasoactive intestinal polypeptide nor substance P exerted any inhibitory or stimulatory action on GVB. These results suggest a limited but definite inhibitory action of SRIF on GVB of porcine ova.
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: M
authors.initials: M
authors.lastname: Irahara
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Ohno
authors.forename: E
authors.initials: E
authors.lastname: Hosoi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D007106:Immune Sera; D008540:Meiosis / Q000187:drug effects*; D006080:Ovarian Follicle / Q000187:drug effects; D010063:Ovum / Q000187:drug effects*; D010121:Oxytocin / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D013004:Somatostatin / Q000494:pharmacology*; D013552:Swine; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
chemical_list: D003470:Culture Media; D007106:Immune Sera; D010455:Peptides; D014660:Vasoactive Intestinal Peptide; D010121:Oxytocin; D013004:Somatostatin