Competitive idiotype--anti-idiotype immunoassay for adenosine deaminase binding protein in urine.
This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reproducible as an earlier described "sandwich"-type immunoassay for ABP (Clin Chem 31: 679-683, 1985). Evaluation of urine samples from normal subjects and from patients showed increased concentrations of ABP in patients with renal disease.
Thompson
R E
RE
Hewitt
C R
CR
Piper
D J
DJ
Hansen
W P
WP
Rubin
R H
RH
Tolkoff-Rubin
N E
NE
Barrett
M C
MC
Nelles
M J
MJ
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal / Q000032:analysis; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000276:immunology / Q000652:urine*; D018819:Dipeptidyl Peptidase 4; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007130:Immunoglobulin Idiotypes; D007683:Kidney Tubular Necrosis, Acute / Q000652:urine
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D007130:Immunoglobulin Idiotypes; D018819:Dipeptidyl Peptidase 4