title: Isolation from porcine intestinal extracts of a cholecystokinin-like peptide and a peptide with homology to cytochrome oxidase polypeptide VII and chymodenin.
abstract: A number of different forms of cholecystokinin (CCK) exist in the brain and intestine. Gel permeation and ion exchange chromatography and high performance liquid chromatography have been used to isolate a peptide from partially purified porcine intestinal extracts with N-terminal homology to porcine brain CCK-58. This peptide contracted both the guinea pig ileum longitudinal muscle and gallbladder muscle and these responses were inhibited by dibutyryl cyclic GMP or proglumide. The potency was approximately 1/100 of that of CCK-8. The reason for this low potency is unclear, but it is possible that a critical part of the biologically active region is modified or that it is a truncated form of CCK-58. A further peptide was isolated with a sequence homologous to cytochrome oxidase polypeptide VII and chymodenin.
authors.affiliation: MRC Regulatory Peptide Group, Department of Physiology, University of British Columbia, Vancouver, Canada.
authors.forename: C H
authors.initials: CH
authors.lastname: McIntosh
authors.forename: M A
authors.initials: MA
authors.lastname: Dahl
authors.forename: Y N
authors.initials: YN
authors.lastname: Kwok
authors.forename: V
authors.initials: V
authors.lastname: Mutt
authors.forename: N
authors.initials: N
authors.lastname: Spruston
authors.forename: J C
authors.initials: JC
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002766:Cholecystokinin / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003576:Electron Transport Complex IV / Q000302:isolation & purification*; D005260:Female; D006168:Guinea Pigs; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D010455:Peptides; C007058:chymodenin; D002766:Cholecystokinin; D003576:Electron Transport Complex IV