Effect of cholesterol epoxides on the inhibition of intercellular communication and on mutation induction in Chinese hamster V79 cells.
Cholesterol, cholesterol-5 alpha, 6 alpha-epoxide, cholesterol-5 beta, 6 beta-epoxide and cholestane-3 beta,5 alpha,6 beta-triol were tested for their ability to induce mutations at the Na+/K+-ATPase loci of the Chinese hamster V79 cells. None of these compounds induced ouabain-resistant mutations compared to the background mutation frequency in the control cells. These compounds were further tested for their ability to inhibit intercellular communication, using the Chinese hamster V79 cell metabolic cooperation assay. The diastereomeric epoxides and cholestane-triol, but not cholesterol, were found to be inhibitors of intercellular communication in a manner similar to other known tumor promoters.
Chang
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CC
Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing 48824.
Jone
C
C
Trosko
J E
JE
Peterson
A R
AR
Sevanian
A
A
D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D002460:Cell Line; D002777:Cholestanols / Q000494:pharmacology*; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D003864:Depression, Chemical; D005347:Fibroblasts / Q000187:drug effects*; D007365:Intercellular Junctions / Q000187:drug effects; D008168:Lung; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009152:Mutagenicity Tests; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics
D002777:Cholestanols; C000474:cholestane-3,5,6-triol; C002415:cholesterol alpha-oxide; D008769:Methylnitronitrosoguanidine; D002784:Cholesterol; D000254:Sodium-Potassium-Exchanging ATPase