title: 31P magnetization transfer in the phosphoglyceromutase-enolase coupled enzyme system.
abstract: The rate of exchange of phosphoryl groups between 2-phosphoglycerate, 3-phosphoglycerate and phosphoenolpyruvate by the coupled phosphoglyceromutase-enolase enzyme system using one- and two-dimensional 31P NMR spectroscopy was measured. Magnetization exchange in one-dimensional experiments was achieved by saturation transfer with selective irradiation at both one and two sites in this three-site exchange system using the DANTE pulse sequence. The two-dimensional magnetization exchange experiment avoids the need to selectively saturate at one or more frequencies which may be difficult in complex exchange systems. Analysis of the two-dimensional exchange experiment by the back transformation method yielded exchange rate constants in good agreement with the saturation transfer method.
authors.affiliation: Department of Biochemistry, University of Sydney, NSW, Australia.
authors.forename: B E
authors.initials: BE
authors.lastname: Chapman
authors.forename: I M
authors.initials: IM
authors.lastname: Stewart
authors.forename: B T
authors.initials: BT
authors.lastname: Bulliman
authors.forename: G L
authors.initials: GL
authors.lastname: Mendz
authors.forename: P W
authors.initials: PW
authors.lastname: Kuchel
mesh_terms: D000818:Animals; D005988:Glyceric Acids / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010736:Phosphoglycerate Mutase / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011817:Rabbits
chemical_list: D005988:Glyceric Acids; D010710:Phosphates; C008885:2-phosphoglycerate; D010728:Phosphoenolpyruvate; C005156:3-phosphoglycerate; D010770:Phosphotransferases; D010751:Phosphopyruvate Hydratase; D010736:Phosphoglycerate Mutase