title: Comparison of a protein-stabilized Rotazyme II test, with standard Rotazyme II, and electron microscopy for detection of rotavirus.
abstract: We compared the performance of Rotazyme II with protein-stabilized diluent to standard Rotazyme II and to direct electron microscopy, for the detection of rotavirus. Sensitivity and specificity were: standard Rotazyme II, 79 and 100%, respectively; Rotazyme II with protein stabilizers, both 100%, and electron microscopy, 75 and 100%, respectively. Commercial availability of this product should improve rotavirus detection for laboratories utilizing Rotazyme II methodology.
authors.affiliation: Department of Internal Medicine, Orlando Regional Medical Center, Florida 32806.
authors.forename: R
authors.initials: R
authors.lastname: Brooks
authors.forename: L
authors.initials: L
authors.lastname: Brown
authors.forename: R
authors.initials: R
authors.lastname: Franklin
mesh_terms: D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000382:microbiology*
chemical_list: D011933:Reagent Kits, Diagnostic