[Isolation of a homogeneous functionally active beta-adrenergic receptor from bovine cerebellum using lauroyl sucrose. Effect of trypsin on receptor activity].
The synthesis of lauroyl sucrose capable of solubilizing 100% of beta-adrenergic receptors from bovine cerebellum membranes has been carried out. The preparative procedure for isolation of homogeneous beta-adrenergic receptors including affinity chromatography on the novel support, oxprenolol-Sepharose, is described. According to SDS-PAAG electrophoresis data, the Mr value for the beta-adrenergic receptor is 61 kD. The purified beta-adrenergic receptor can interact with the purified GTP-binding regulatory protein of adenylate cyclase (Gs) after their reconstitution into liposomes. Trypsin treatment of the purified receptor does not interfere with its functional properties, nor does it change the hydrodynamic parameters under non-denaturing conditions despite the fact that the polypeptide chain of the receptor is cleaved by trypsin.
Voeĭkov
V L
VL
Udovichenko
I P
IP
Kiselev
O G
OG
Tagaev
A A
AA
Kovalevich
T E
TE
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002531:Cerebellum / Q000032:analysis*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D011869:Radioligand Assay; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000302:isolation & purification*; D013395:Sucrose / Q000031:analogs & derivatives*; D014357:Trypsin / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D011943:Receptors, Adrenergic, beta; C041567:sucrose monopalmitate; D013395:Sucrose; D014357:Trypsin
Vydelenie gomogennogo funktional'no aktivnogo beta- adrenoretseptora iz mozzhechka byka s ispol'zovaniem lauroilsakharozy. Vliianie tripsina na aktivnost'' retseptora.