Single-step purification of two hyperglycaemic neurohormones from the sinus gland of Procambarus bouvieri. Comparative peptide mapping by means of high-performance liquid chromatography.
A crude aqueous extract from 2000 sinus glands of the Mexican crayfish Procambarus bouvieri (Ortmann) was fractionated on a muBondapak-Phenyl column. Two isoforms of the crustacean hyperglycaemic hormone, designated CHH-B and CHH-C in order of elution, were isolated in pure form. Their biochemical characterization showed a remarkable degree of homology. A tryptic digest of each isohormone was fractionated on an Ultrasphere-ODS column. Only one tryptic peptide in CHH-C was eluted later than its homologous peptide in CHH-B. On acid hydrolysis both tryptic peptides had the same composition but, as they contain Asp and Glu, we suspect that the difference residues in a double reciprocal amidation-deamidation of two acidic residues.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003400:Astacoidea / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005088:Exocrine Glands / Q000032:analysis*; D005260:Female; D006868:Hydrolysis; D007202:Indicators and Reagents; D008297:Male; D018377:Neurotransmitter Agents / Q000302:isolation & purification*; D010449:Peptide Mapping; D011506:Proteins / Q000032:analysis; D014020:Tissue Extracts / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D007202:Indicators and Reagents; D018377:Neurotransmitter Agents; D011506:Proteins; D014020:Tissue Extracts; D014357:Trypsin