Ammonium assimilation in Rhizobium phaseoli by the glutamine synthetase-glutamate synthase pathway.
Evidence from in vitro and in vivo studies showed that in Rhizobium phaseoli ammonium is assimilated by the glutamine synthetase (GS)-glutamate synthase NADPH pathway. No glutamate dehydrogenase activity was detected. R. phaseoli has two GS enzymes, as do other rhizobia. The two GS activities are regulated on the basis of the requirement for low (GSI) or high (GSII) ammonium assimilation. When the 2-oxoglutarate/glutamine ratio decreases, GSI is adenylylated. When GSI is inactivated, GSII is induced. However, induction of GSII activity varied depending on the rate of change of this ratio. GSII was inactivated after the addition of high ammonium concentrations, when the 2-oxoglutarate/glutamine ratio decreased rapidly. Ammonium inactivation resulted in alteration of the catalytic and physical properties of GSII. GSII inactivation was not relieved by shifting of the cultures to glutamate. After GSII inactivation, ammonium was excreted into the medium. Glutamate synthase activity was inhibited by some organic acids and repressed when cells were grown with glutamate as the nitrogen source.
D003470:Culture Media; D004790:Enzyme Induction; D005970:Glutamate Synthase / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012231:Rhizobium / Q000201:enzymology* / Q000254:growth & development; D000637:Transaminases / Q000378:metabolism*
D003470:Culture Media; D005971:Glutamates; D007656:Ketoglutaric Acids; D000644:Quaternary Ammonium Compounds; D005973:Glutamine; D005970:Glutamate Synthase; D000637:Transaminases; D005974:Glutamate-Ammonia Ligase