The time-course of appearance and net accumulation of horseradish peroxidase (HRP) presented orally to juvenile carp Cyprinus carpio (L.).
A sensitive enzyme-linked immunosorbent assay (ELISA) technique was used in order to determine horseradish peroxidase (HRP) uptake from the carp gut. HRP was detected in blood plasma and various tissues within 15 min of oral intubation. The time-course of net accumulation (uptake-degradation) over a 2 hr period was recorded. The presence of HRP reached a maximum in the body tissues approximately 1 hr after intubation and on a microgram/g wet weight basis the order of accumulation within the tissues was spleen greater than kidney greater than liver. The total organ accumulation (net) was in the order liver greater than kidney greater than spleen.
D000284:Administration, Oral; D000818:Animals; D002347:Carps / Q000378:metabolism*; D003530:Cyprinidae / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase / Q000008:administration & dosage / Q000378:metabolism*; D007408:Intestinal Absorption; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
D006735:Horseradish Peroxidase; D010544:Peroxidases