Inhibitory effect of taurolipids on Clostridium perfringens sialidase.
Taurolipids A and B, which are detergent-type compounds isolated from protozoan Tetrahymena cells, were demonstrated to inhibit strongly the activity of Clostridium perfringens sialidase. On addition of 280 pmol of taurolipid B to 20 mU of the enzyme, the sialidase activity was decreased to 7% of the original activity at pH 5.1 as the optimum pH. The inhibition was non-competitive. Effective inhibition was observed at the acidic region from the isoelectric point of the sialidase, and at a low ionic strength. Both the long chain acyl and sulfonic acid groups of taurolipids were required for the inhibition of the sialidase activity. A mechanism is postulated for the inhibition.
D003016:Clostridium perfringens / Q000201:enzymology*; D005227:Fatty Acids / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
D005227:Fatty Acids; C053551:taurolipid A; C053552:taurolipid B; D013654:Taurine; D009439:Neuraminidase