Relative zinc-binding activities of ligands in the cytosol of rat small intestine.
Relative zinc-binding activities of high-molecular-weight zinc-binding ligand (HMW-ZBL), metallothionein (MT) and low-molecular-weight zinc-binding ligand (LMW-ZBL) in the cytosols of rat small intestines under various experimental conditions were examined. Zinc-binding activities of MT decreased and those of LMW-ZBL increased in the intestinal cytosols from most of the experimental rat groups after incubating at 37 degrees C for 2 hr. The relative zinc-binding activity of MT increased with increasing doses of injected zinc and decreased with orally-administered zinc. Isolated MT did not lose zinc-binding activity during incubation at 37 degrees C for 48 hr, but moved from the MT eluting peak to the LMW-ZBL eluting peak after 1 week.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005260:Female; D007421:Intestine, Small / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D015032:Zinc / Q000378:metabolism*
D002352:Carrier Proteins; C017800:zinc-binding protein; D015032:Zinc