A new method for measuring the free fraction of cyclosporin in plasma by equilibrium dialysis.
The free fraction of cyclosporin in plasma has been measured by equilibrium dialysis in steel chambers. Equilibration time was 16 h. The intra-assay coefficient of variation was 7%. The free fraction remained constant in plasma samples frozen for up to 6 months, and was independent of the total concentration within the therapeutic range.
D000327:Adsorption; D003524:Cyclosporins / Q000097:blood*; D003956:Dialysis / Q000295:instrumentation; D006801:Humans
D003524:Cyclosporins