Serological diagnosis of primary Sjögren's syndrome by means of human recombinant La (SS-B) as nuclear antigen.
Human recombinant La nucleoprotein was purified from cultures of Escherichia coli containing a vector with a 1.4 kilobase cDNA encoding La; the nucleoprotein was used to test for antinuclear antibodies (ANA) to La. Serum samples from 260 patients with autoimmune diseases associated with ANA and 100 healthy subjects were tested by an enzyme-linked immunosorbent assay (ELISA). Samples from 47 (94%) of 50 patients with primary Sjögren's syndrome and 1 (7%) of 14 patients with secondary Sjögren's syndrome reacted with the recombinant La. No reactivity was demonstrated in 196 patients with other ANA-associated autoimmune diseases or in 100 healthy subjects. The study confirms the high correlation between ANA, anti-La, and primary Sjögren's syndrome and shows how gene cloning can provide large quantities of human autoantigens for use in highly specific and sensitive diagnostic assays.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D001324:Autoantigens; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D012859:Sjogren's Syndrome / Q000175:diagnosis* / Q000276:immunology; D000097585:SS-B Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D000097585:SS-B Antigen