Inhibition by some phenolic antioxidants of Ca2+ uptake and neurotransmitter release from brain synaptosomes.
We present observations on the action of the phenolic antioxidants 2,3-tert-butyl-4-methoxyphenol (BHT); 2,6-di-tert-butyl-4-methylphenol (BHA); n-propyl-gallate and nordihydroguaiaretic acid (NDGA) on the function of guinea pig cerebral cortex synaptosomes. While the Ca2+ uptake observed under non depolarizing conditions is not affected by these agents, the depolarization induced Ca2+ uptake is strongly inhibited. Similarly the phenolic antioxidants studied inhibit the Ca2+ plus depolarization induced exocytosis of GABA and glutamate. These results which are similar to those previously obtained in blood platelets (Alexandre, A. et. al. Biochem. Biophys. Res. Comm. 139, 509-514, 1986), may indicate that free radical intermediates are involved in stimulus-secretion coupling.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006168:Guinea Pigs; D009637:Masoprocol / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology*; D011435:Propyl Gallate / Q000494:pharmacology; D013574:Synaptosomes / Q000166:cytology* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000975:Antioxidants; D005971:Glutamates; D018377:Neurotransmitter Agents; D010636:Phenols; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D009637:Masoprocol; D011435:Propyl Gallate; D002118:Calcium