title: The toxicity of phomopsin for sheep.
abstract: Pure phomopsin was administered to young Merino x Border Leicester wethers by single subcutaneous (SC) and by single and multiple intraruminal (IR) injection. The toxicity after IR injection was influenced by the size of individual doses and the time over which the total dose was given. At high levels of ingestion the toxicity of phomopsin may be limited by absorption rates; with low daily doses the capacity to repair liver damage may be sufficient to prevent cumulative effects. By SC injection a single dose of 10 micrograms/kg approximated the LD50. By IR injection the overall clinical, biochemical and histological responses closest to these of this SC dose resulted from a single dose of 1,000 micrograms/kg. The same total dose administered at daily rates of 50 or 200 micrograms/kg was more toxic and killed all sheep. A single dose of 500 micrograms/kg caused significant liver damage, but no deaths. Single doses of 125 and 250 micrograms/kg and repeated daily doses of 12.5 micrograms/kg over 16 weeks caused no detectable tissue damage. Inappetence was the most sensitive indicator of phomopsin toxicity. About 10% of the sheep differed substantially from the rest of the flock in their susceptibility to phomopsin poisoning.
mesh_terms: D000284:Administration, Oral / Q000662:veterinary; D000818:Animals; D001066:Appetite / Q000187:drug effects; D001219:Aspartate Aminotransferases / Q000097:blood; D001647:Bile Acids and Salts / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury; D004435:Eating / Q000187:drug effects; D007279:Injections, Subcutaneous / Q000662:veterinary; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008107:Liver Diseases / Q000662:veterinary*; D008297:Male; D008938:Mitosis; D009183:Mycotoxins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D012756:Sheep; D012757:Sheep Diseases / Q000139:chemically induced*; D013448:Sulfobromophthalein; D005723:gamma-Glutamyltransferase / Q000097:blood; D009842:Oligopeptides
chemical_list: D001219:Aspartate Aminotransferases; D001647:Bile Acids and Salts; D001663:Bilirubin; D009183:Mycotoxins; D013448:Sulfobromophthalein; D005723:gamma-Glutamyltransferase; C035753:phomopsin; D009842:Oligopeptides