title: Regulation of gastric somatostatin secretion in the mouse by luminal acidity: a local feedback mechanism.
abstract: The present study was designed to determine whether somatostatin secretion induced by histamine or pentagastrin in the isolated luminally perfused mouse stomach was a direct effect of the secretagogues on gastric somatostatin cells or an indirect effect mediated by the increase in luminal acidity. Perfusion of the lumen with exogenous acid (80-480 nmol/min) caused an increase in somatostatin secretion in proportion to the increase in luminal acidity. The increase in somatostatin secretion was resistant to tetrodotoxin and attained maximal levels (61.6% +/- 8.7% above basal level) similar to those elicited by maximal doses of secretagogues. Conversely, neutralization of basal acid secretion with bicarbonate (20-160 nmol/min) caused a decrease in somatostatin secretion in proportion to the decrease in luminal acidity. Similarly, neutralization of the secretagogue-induced increments in acid secretion with bicarbonate or inhibition of the increments with cimetidine abolished the corresponding increments in somatostatin secretion. It is proposed that acid-induced release of somatostatin in proximity to parietal cells serves as a negative feedback mechanism restraining acid secretion.
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D002927:Cimetidine / Q000494:pharmacology; D005246:Feedback; D005744:Gastric Acid / Q000378:metabolism / Q000502:physiology*; D005753:Gastric Mucosa / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D010418:Pentagastrin / Q000494:pharmacology; D013004:Somatostatin / Q000378:metabolism*
chemical_list: D001639:Bicarbonates; D013004:Somatostatin; D002927:Cimetidine; D006632:Histamine; D010418:Pentagastrin