Inhibition of plant acetyl-coenzyme A carboxylase by the herbicides sethoxydim and haloxyfop.
Incorporation of [14C]acetate or [14C]pyruvate into fatty acids in isolated corn seedling chloroplasts was inhibited 90% or greater by 10 microM sethoxydim or 1 microM haloxyfop. At these concentrations, neither sethoxydim nor haloxyfop inhibited [14C]acetate incorporation into fatty acids in isolated pea chloroplasts. Sethoxydim (10 microM) and haloxyfop (1 microM) did not inhibit incorporation of [14C]malonyl-CoA into fatty acids in cell free extracts from corn tissue cultures. Acetyl coenzyme A carboxylase (EC 6.4.1.2) from corn seedling chloroplasts was inhibited by both sethoxydim and haloxyfop, with I50 values of 2.9 and 0.5 microM, respectively. This enzyme in pea was not inhibited by 10 microM sethoxydim or 1 microM haloxyfop.
D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors*; D002736:Chloroplasts / Q000378:metabolism; D003510:Cyclohexanes / Q000494:pharmacology*; D003512:Cyclohexanones / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000096:biosynthesis; D006540:Herbicides / Q000494:pharmacology*; D066298:In Vitro Techniques; D008025:Ligases / Q000037:antagonists & inhibitors*; D011725:Pyridines / Q000494:pharmacology*; D003313:Zea mays / Q000201:enzymology*
D003510:Cyclohexanes; D003512:Cyclohexanones; D005227:Fatty Acids; D006540:Herbicides; D011725:Pyridines; C049437:sethoxydim; C054176:haloxyfop; D008025:Ligases; D000103:Acetyl-CoA Carboxylase