Crohn's disease-isolated mycobacteria are identical to Mycobacterium paratuberculosis, as determined by DNA probes that distinguish between mycobacterial species.
DNA extracted from an unclassified Crohn's disease-isolated Mycobacterium strain was cloned. The recombinant clones were radiolabeled and hybridized to restriction digests of mycobacterial DNA transferred to nylon membranes. Restriction fragment length polymorphisms (RFLPs) were identified that distinguished between mycobacterial DNA samples. Quantitative estimates of frequencies of DNA base substitution were also obtained. No RFLPs were detected between the DNA of three unclassified Crohn's disease-isolated mycobacteria and Mycobacterium paratuberculosis, although several RFLPs were detected that distinguished between M. paratuberculosis and both M. avium complex serovars 2 and 5. The frequency of DNA base substitution between M. paratuberculosis and M. avium complex serovar 2 was measured as 0.87 (+/- 1.2)%.
D003001:Cloning, Molecular; D003424:Crohn Disease / Q000382:microbiology*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D006801:Humans; D009161:Mycobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009162:Mycobacterium avium / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010283:Paratuberculosis / Q000382:microbiology; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI