[Effect of forskolin and VIP on the release of somatostatin and the cAMP content of cultured rat diencephalon neurons].
Forskolin, a direct activator of adenylate cyclase, stimulates somatostatin release in dispersed fetal diencephalic cells in culture (10 j). It was found that concentrations ranging from 10(-8) M to 10(-4) M increase the release of somatostatin in a dose-dependent manner, as well as the formation of intracellular cyclic AMP. Furthermore, VIP (10(-6) M) which produces a significant (p less than 0.03) elevation of SRIF release at 30 min of incubation, also induces a prompt increase of intracellular cyclic AMP (10 min). These results suggest that VIP could stimulate the somatostatin release through a cyclic AMP-dependent mechanism.
D000818:Animals; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D004027:Diencephalon / Q000187:drug effects / Q000378:metabolism*; D005333:Fetus; D007700:Kinetics; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013004:Somatostatin / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D000242:Cyclic AMP
Effets de la forskoline et du VIP sur la libération de somatostatine et le contenu en AMPc de neurones diencéphaliques de rat en culture.