title: Transglutaminase modification of rhodopsin in retinal rod outer segment disk membranes.
abstract: Rhodopsin in rod outer segment disk membranes was enzymatically modified by erythrocyte transglutaminase, which linked small primary amines to glutamine residues. In order to avoid formation of protein crosslinks, rhodopsin was first reductively methylated to modify its lysines. From 1.9 to 2.5 mol of putrescine, ethanolamine, or dinitrophenylcadaverine were incorporated into rhodopsin by transglutaminase during 16 h reaction time. A maximum of 3.5 mol of [14C]putrescine was incorporated per mole of rhodopsin during 48 h. Essentially all of the rhodopsin sequence containing the putrescine could be removed by limited proteolysis of the membranes by thermolysin. Glutamine residues in positions 236, 237, 238, and 344 were modified to approximately equal extents, as determined by isolation of the cyanogen bromide peptides of modified rhodopsin followed by further subdigestion of the peptides. The modified glutamine residues are located in the helix V-VI (or F1-F2) connecting loop and in the carboxyl-terminal region of rhodopsin.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D006801:Humans; D008566:Membranes / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D010786:Photoreceptor Cells / Q000378:metabolism*; D012168:Retinal Pigments / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism*; D012374:Rod Cell Outer Segment / Q000378:metabolism*; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D012168:Retinal Pigments; D012243:Rhodopsin; D011503:Transglutaminases