Fluoride inhibition of proton-translocating ATPases of oral bacteria.
The ATPases of isolated membranes of lactic acid bacteria were found to be inhibited by fluoride in a complex manner. Among the enzymes tested, that of Streptococcus mutans GS-5 was the most sensitive to fluoride, and the initial rate of hydrolysis of ATP was reduced 50% by approximately 3 mM fluoride. The enzyme of Lactobacillus casei ATCC 4646 was the most resistant, and about 25 mM fluoride was required for 50% inhibition. The response to fluoride appeared to involve reversible, noncompetitive inhibition during short exposure to low levels of fluoride and nonreversible inhibition at higher fluoride levels. In addition, kinetic studies of the effects of fluoride on the enzymes of membranes of S. mutans and L. casei indicated that reversible inhibition was at least partly overcome at high levels of either ATP or Mg. The effects of pH on fluoride inhibition of ATPases were markedly different from the effects of pH on inhibition of acid/base regulation of intact cells by fluoride. It appeared that formation of HF was not required for inhibition of the ATPases. F1 ATPases isolated from the membranes by washing with buffers of low ionic strength proved to be less sensitive to fluoride than the membrane-associated F1F0 holoenzymes, and it was concluded that the F0 or membrane sector of the holoenzyme is involved in fluoride inhibition.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D001410:Bacillus megaterium / Q000201:enzymology; D002462:Cell Membrane / Q000201:enzymology; D003773:Dental Plaque / Q000382:microbiology; D005459:Fluorides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000201:enzymology; D009055:Mouth / Q000382:microbiology*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D013291:Streptococcus / Q000201:enzymology*
D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases; D005459:Fluorides