Determination of azelastine and desmethylazelastine in human plasma by high-performance liquid chromatography.
A manual-injection liquid chromatographic method using fluorescence detection permitted determination of a new antiasthmatic drug, azelastine, and its desmethyl metabolite extracted from human plasma. Reliable quantitation was achieved to at least 0.3 ng/ml for each analyte. No interference was seen in co-chromatography of sixteen other substances, which were potential co-medications (or their metabolites) as used in standard asthma or allergy treatment.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004347:Drug Interactions; D006634:Histamine H1 Antagonists / Q000097:blood*; D006801:Humans; D010793:Phthalazines / Q000097:blood*; D011724:Pyridazines / Q000097:blood*; D013050:Spectrometry, Fluorescence
D006634:Histamine H1 Antagonists; D010793:Phthalazines; D011724:Pyridazines; C054114:desmethylazelastine; C020976:azelastine