Association of liposome-entrapped [3H]methotrexate with thioglycollate-elicited macrophages in-vitro.
The association of free or liposome-entrapped [3H]methotrexate [( 3H]MTX) with thioglycollate-elicited macrophages was investigated in-vitro. [14C]Cholesteryl oleate was incorporated into the liposomes as a lipid marker. [3H]MTX association with the macrophages was 5 to 9-fold higher with liposome-entrapped [3H]MTX than with the free drug. Macrophage-liposome association was biphasic, temperature-dependent and saturable at high liposomal lipid concentration. A high liposome cholesterol (CH) content or the presence of 2,4-dinitrophenol or colchicine also reduced macrophage-liposome association.
D019297:2,4-Dinitrophenol; D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D066298:In Vitro Techniques; D008081:Liposomes / Q000008:administration & dosage / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008727:Methotrexate / Q000008:administration & dosage / Q000378:metabolism*; D011817:Rabbits; D013696:Temperature; D013864:Thioglycolates / Q000494:pharmacology
D004140:Dinitrophenols; D008081:Liposomes; D013864:Thioglycolates; D019297:2,4-Dinitrophenol; D003078:Colchicine; D008727:Methotrexate