title: Postaggregative differentiation induction by cyclic AMP in Dictyostelium: intracellular transduction pathway and requirement for additional stimuli.
abstract: Cyclic AMP induces postaggregative differentiation in aggregation competent cells of Dictyostelium by interacting with cell surface cAMP receptors. We investigated the transduction pathway of this response and additional requirements for the induction of postaggregative differentiation. Optimal induction of postaggregative gene expression requires that vegetative cells are first exposed to 2-4 hr of nanomolar cAMP pulses, and subsequently for 4-6 hr to steady-state cAMP concentrations in the micromolar range. Cyclic AMP pulses, which are endogenously produced before and during aggregation, induce full responsiveness to cAMP as a morphogen. The transduction pathway from the cell surface cAMP receptor to postaggregative gene expression may involve Ca2+ ions as intracellular messengers. A cAMP-induced increase in intracellular cAMP or cGMP levels is not involved in the transduction pathway.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000946:Antigens, Fungal / Q000235:genetics; D002118:Calcium / Q000502:physiology; D002449:Cell Aggregation; D002454:Cell Differentiation; D000242:Cyclic AMP / Q000502:physiology*; D006152:Cyclic GMP / Q000502:physiology; D004023:Dictyostelium / Q000166:cytology* / Q000502:physiology; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D006162:Guanylate Cyclase / Q000502:physiology; D009955:Ornithine Decarboxylase / Q000235:genetics; D006005:Phosphorylases / Q000235:genetics; D013217:Starvation / Q000503:physiopathology
chemical_list: D000946:Antigens, Fungal; D000242:Cyclic AMP; D006005:Phosphorylases; D009955:Ornithine Decarboxylase; D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D002118:Calcium