HLA-DQ heterogeneity among HLA-DRw11(5) haplotypes.
Class II molecular variation among a panel of ten HLA-DRw11(5) homozygous cell lines (HCL) was investigated by analysis of restriction fragment length polymorphisms in genomic DNA. Hybridization of Bgl II, Hind III, and Taq I digested cellular DNA with DQ alpha and DQ beta cDNA probes identified a clustering of characteristic polymorphisms. Considerable diversity was observed between the HLA-DRw11(5), DQw3 positive haplotypes studied, as well as compared to a DRw11(5), DQw1 positive haplotype. In contrast to the observed DQ genomic variability, hybridization with a DR beta probe revealed relatively limited diversity. The molecular heterogeneity seen by genomic restriction fragment analysis illustrates the presence of genomic polymorphisms, particularly within HLA-DQ-related genes among a family of DRw11(5)-related haplotypes.
D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006681:HLA-D Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059810:HLA-DR Serological Subtypes; D006238:Haploidy; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D006681:HLA-D Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C080766:HLA-DR11 antigen; D000949:Histocompatibility Antigens Class II; D003101:Collodion