Rat atrial natriuretic peptide (99-126) stimulates guanylate cyclase activity in rat subfornical organ and choroid plexus.
The effect of rat atrial natriuretic peptide (99-126) (rANP) on cyclic guanosine 3',5'-monophosphate production was investigated in two brain areas, the subfornical organ and the choroid plexus. rANP activated guanylate cyclase in crude homogenates of rat subfornical organ and choroid plexus in concentration and time dependent fashions. A 2-fold stimulation of the enzyme was obtained with 100 nM rANP and a half-maximal stimulation with a 10 nM dose. Our results further support the hypothesis that cGMP mediates the central action of ANP through the activation of specific receptors in localized target sites such as the subfornical organ and choroid plexus.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002831:Choroid Plexus / Q000201:enzymology*; D006162:Guanylate Cyclase / Q000378:metabolism*; D008297:Male; D009490:Neurosecretory Systems / Q000201:enzymology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D013268:Stimulation, Chemical; D013356:Subfornical Organ / Q000201:enzymology*
D010446:Peptide Fragments; D012965:Sodium Chloride; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase