[Microbial inhibitors of phospholipase C].
Inhibitors of the Clostridium perfringens phospholipase C were prepared from the filtrates of the culture liquids of Streptomyces saracetidus and Streptomyces species using soluble and cross-linked polyelectrolytes. The technological scheme of isolation involves ultrafiltration. The inhibitors produced by the two strains had different chemical nature. The preparation obtained from Str. saraceticus was proved to be a complex of inhibitors that were separated by gel-chromatography into a major polypeptide with a molecular weight of 5500-6500 and a low-molecular weight glycopeptide. The inhibitor obtained from Str. species was found to be a high-molecular weight protein.
D002850:Chromatography, Gel; D003016:Clostridium perfringens / Q000201:enzymology*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D013302:Streptomyces / Q000201:enzymology*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors*
D010738:Type C Phospholipases
Ingibitory fosfolipazy C mikrobnogo proiskhozhdeniia.