Biological effect of anti-fucosyl GM1 ganglioside antibody on cyclic adenosine 3',5'-monophosphate production in FRTL5 rat thyroid cells.
Addition of specific anti-fucosyl GM1 antibody raised in a rabbit caused dose-dependent inhibition of endogenous and thyrotropin (TSH)- or thyroid stimulating antibody-stimulated cyclic adenosine 3',5'-monophosphate (cAMP) production in cultured FRTL5 rat thyroid cells. Further, the antibody inhibited the cAMP increase induced by prostaglandin E1 and forskolin. However, anti-fucosyl GM1 antibody did not affect the binding of [125I]bovine TSH to solubilized porcine thyroid TSH receptor or to FRTL5 cells. In conclusion, fucosyl GM1 is one of the specific membrane components of thyrocytes and appears to be involved in adenylate cyclase stimulation or cAMP generation. Further, the biological effects of the ganglioside do not seem to be mediated by the TSH receptor, suggesting a post receptor mechanism.
D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D000918:Antibody Specificity; D001665:Binding Sites; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G; D018828:Immunoglobulins, Thyroid-Stimulating; D051381:Rats; D011989:Receptors, Thyrotropin / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology
D007074:Immunoglobulin G; D018828:Immunoglobulins, Thyroid-Stimulating; D011989:Receptors, Thyrotropin; D005576:Colforsin; D005677:G(M1) Ganglioside; C039123:fucosyl GM1 ganglioside; D013972:Thyrotropin; D000242:Cyclic AMP; D000527:Alprostadil