gamma-Glutamyl transpeptidase demonstrated in tissue sections embedded in glycol methacrylate resin.
A method is described for the histochemical demonstration of gamma-glutamyl transpeptidase in tissue sections embedded in glycol methacrylate at low temperature. Enzyme activity was preserved by a short (3 h) fixation of tissue in 4% paraformaldehyde at 4 degrees C prior to embedding at 4 degrees C. Tissue embedded in glycol methacrylate combined good morphology with accurate enzyme localization. Blocks of the embedded tissue could be stored at room temperature for at least 3 months without loss of enzyme activity. The resin is non-fluorescent, allowing the use of the fluorescent coupling agent 5-nitrosalicylaldehyde to visualize the reaction product.
D000179:Acrylates; D000818:Animals; D005260:Female; D005404:Fixatives; D006652:Histological Techniques; D007413:Intestinal Mucosa / Q000201:enzymology; D007583:Jejunum / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008689:Methacrylates; D051379:Mice; D010179:Pancreas / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D000179:Acrylates; D005404:Fixatives; D008689:Methacrylates; C005044:hydroxyethyl methacrylate; D005723:gamma-Glutamyltransferase