Metabolic cooperation in SK-HEP-1 human hepatoma cells following treatment with benzodiazepine tranquilizers.
The ability of three benzodiazepine tranquilizers, diazepam, oxazepam and chlordiazepoxide chlorhydrate to block metabolic cooperation between human hepatoma cells was analysed using autoradiographical monitoring. Independent tests were performed with compound concentrations varying from 1 to 50 micrograms/ml. The results showed that at a toxic dose of 50 micrograms/ml diazepam and oxazepam markedly inhibited metabolic cooperation and that oxazepam remained effective at a lower, non-toxic, dose (10 micrograms/ml). Chlordiazepoxide chlorhydrate was found to be less toxic than diazepam and oxazepam and did not interfere with metabolic cooperation at any concentration up to 50 micrograms/ml.
D014151:Anti-Anxiety Agents / Q000633:toxicity*; D001569:Benzodiazepines; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002450:Cell Communication / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism
D014151:Anti-Anxiety Agents; D001569:Benzodiazepines