Virus-vector-host relationships of Aedes stimulans and Jamestown Canyon virus in a northern Indiana enzootic focus.
Collections of hematophagous Diptera at the Kingsbury State Fish and Wildlife Area in northern Indiana between 1982 and 1984 yielded 118,972 mosquitoes from which 5 isolates of Jamestown Canyon virus and 3 isolates of trivittatus virus were obtained. All Jamestown Canyon isolates were from Aedes stimulans, including 1 from a pool of newly emerged males and 2 from pools of newly emerged females. These 3 isolates suggest that Jamestown Canyon virus is transovarially transmitted by Ae. stimulans. All isolates of trivittatus virus came from pools of Ae. trivittatus. No isolates were obtained from greater than 4,000 tabanids collected along with the mosquitoes those years. Transmission trials with field-collected newly emerged adult female Ae. stimulans demonstrated a mean midgut infection rate of 44%, a disseminated infection rate of 16%, and an oral transmission rate of 12% to suckling mice. Precipitin tests of field-collected bloodfed female mosquitoes indicated that white-tailed deer were the preferred host for numerous mosquito species including Ae. stimulans. The results of this study suggest that Ae. stimulans is a primary vector of Jamestown Canyon virus and that transovarial transmission is the probable overwintering mechanism for this California group virus.
D000330:Aedes / Q000382:microbiology*; D000818:Animals; D002043:Bunyaviridae / Q000502:physiology*; D009033:Culicidae / Q000382:microbiology; D003670:Deer / Q000382:microbiology; D002141:Encephalitis Virus, California / Q000302:isolation & purification / Q000502:physiology*; D004671:Encephalitis, Arbovirus / Q000635:transmission*; D004670:Encephalitis, California / Q000635:transmission*; D006793:Houseflies / Q000382:microbiology; D006801:Humans; D007196:Indiana; D007303:Insect Vectors / Q000382:microbiology*; D012621:Seasons