Detection of scrapie-associated fibrils (SAF) and SAF proteins from scrapie-affected sheep.
Scrapie-associated fibrils (SAF) were detected by negative-stain electron microscopy in the brains (by two different isolation procedures) and spleens of sheep naturally and experimentally infected with scrapie. Although the numbers of SAF varied from case to case, the yield of SAF from brains of naturally affected sheep was lower than that from experimentally affected sheep. SAF-specific, protease-resistant proteins (PrPs) were detected by silver staining and western blot analysis in most samples of brain from experimentally affected sheep. PrPs, however, could be detected in only a limited number of natural cases of sheep scrapie because of the lower yields of SAF. PrPs from sheep SAF appear biochemically and antigenically similar to PrPs from other species infected with unconventional agents. This study further establishes the unique association of SAF and PrPs with natural or experimentally induced scrapie in its natural host.
D000818:Animals; D001921:Brain / Q000382:microbiology; D006224:Cricetinae; D051379:Mice; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D018622:PrP 27-30 Protein; D011328:Prions / Q000302:isolation & purification*; D012608:Scrapie / Q000382:microbiology*; D012756:Sheep / Q000382:microbiology; D013154:Spleen / Q000382:microbiology
D009419:Nerve Tissue Proteins; D011328:Prions; D018622:PrP 27-30 Protein