N-acetylglucosamine binding activity in extracts of adult newt skin.
1. Extracts of the dermis of the adult newt, Notophthalmus viridescens, contain hemagglutination activity which is specifically inhibited by N-acetylglucosamine. 2. The activity is soluble and is associated with a doublet in sodium dodecyl sulfate polyacrylamide gels, the bands of which have relative molecular weights of 51,000 and 57,000 under both reducing and non-reducing conditions. 3. The activity requires magnesium but not calcium, cobalt, or manganese and is inhibited by barium. 4. The activity is also dependent on pH with a pH optimum between 7.0 and 7.6.
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D003720:Densitometry; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004903:Erythrocyte Aggregation; D004912:Erythrocytes / Q000187:drug effects; D005944:Glucosamine / Q000031:analogs & derivatives*; D006384:Hemagglutination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D011817:Rabbits / Q000097:blood; D012455:Salamandridae / Q000378:metabolism*; D012867:Skin / Q000032:analysis*; D014020:Tissue Extracts / Q000378:metabolism* / Q000494:pharmacology
D014020:Tissue Extracts; D004492:Edetic Acid; D005944:Glucosamine; D000117:Acetylglucosamine