title: Complete amino acid sequence of rat liver alcohol dehydrogenase deduced from the cDNA sequence.
abstract: Alcohol dehydrogenase (ADH) catalyzes the rate-determining reaction in the metabolism of ethanol. We report here the complete nucleotide sequence of a cDNA encoding rat liver ADH, and the deduced amino acid (aa) sequence of the protein. The rat enzyme contains a cluster of aa substitutions and an aa insertion in the region between aa residues 111 and 118, which is near the intron-exon junction reported for the human ADH gene. It also contains an additional cysteine in the highly variable region from aa residues 108-125 which may account for the unusual lability of rat ADH compared with ADH from other species.
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D051379:Mice / Q000235:genetics / Q000378:metabolism; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D003062:Codon; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA; D000426:Alcohol Dehydrogenase