Parathyroid hormone stimulation of renal adenylate cyclase in various vertebrate species: evidence for an effect in the frog.
The effect of [Nle8,18,Tyr34]bPTH-(1-34)amide on renal plasma membrane adenylate cyclase, in the presence of 0.1 mM guanylylimidodiphosphate was measured in rat, chicken, frog and trout. All species showed an enrichment of at least 8-fold (relative to homogenate) in the marker enzyme Na+,K+-ATPase. A significant dose-dependent adenylate cyclase stimulation was found in frog, with affinity values similar to those of rat and chicken (ED50=8, 10 and 3 nM, respectively), but not in trout. The frog response was specific since [Nle8,18,Tyr34]bPTH-(3-34)amide strongly inhibited the agonist-stimulated enzyme. These results suggest the existence of a PTH-like substance in anurans acting via cyclic AMP formation in the kidney.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002645:Chickens / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D011893:Rana esculenta / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013045:Species Specificity; D014337:Trout / Q000378:metabolism
D010281:Parathyroid Hormone; D010446:Peptide Fragments; D006165:Guanylyl Imidodiphosphate; C032505:parathyroid hormone (1-34)amide, 8,18-Nle-34-Tyr-, bovine; D000262:Adenylyl Cyclases; D000254:Sodium-Potassium-Exchanging ATPase