Interleukin 1 regulates hematopoietic activity, a role previously ascribed to hemopoietin 1.
A murine in vitro assay was developed to measure potentiation of a proliferative response to suboptimal concentrations of the hematopoietic regulatory molecule granulocyte/macrophage colony-stimulating factor by an immature bone marrow population. The assay, designated the 5-fluorouracil bone marrow proliferation assay, was used to characterize potentiating activity in serum-free culture supernatants of the human tumor cell line HBT 5637. Molecular and biochemical analyses indicated that the HBT 5637-derived potentiating activity could be attributed to interleukin 1 alpha. Serologic analysis using a monoclonal antibody against purified recombinant interleukin 1 alpha proved conclusively that the potentiating activity in HBT 5637 serum-free supernatants is due to interleukin 1 alpha. From these data, the activity of interleukin 1 alpha seems to be the same synergistic activity formerly ascribed to hemopoietin 1.
D000818:Animals; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003114:Colony-Forming Units Assay; D005472:Fluorouracil / Q000494:pharmacology; D006133:Growth Substances / Q000502:physiology*; D006410:Hematopoiesis / Q000187:drug effects*; D016298:Hematopoietic Cell Growth Factors; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D011061:Poly A; D005472:Fluorouracil