Examination of enzyme-altered foci with gamma-glutamyl transpeptidase, aldehyde dehydrogenase, glucose-6-phosphate dehydrogenase, and other markers in methacrylate-embedded liver.
F344 Male rats weighting between 90 and 110 gm were given 90 ppm diethylnitrosamine in their drinking water for 5 weeks. Seven weeks after the administration of carcinogen was completed, the rats were sacrificed and sections of their livers were embedded in methacrylate. Serial sections 2 or 4 micron in thickness demonstrated the presence of gamma-glutamyl transpeptidase, acid phosphatase, adenosine triphosphatase, aldehyde dehydrogenase, alkaline phosphatase, alpha-naphthyl butyrate esterase, DT diaphorase, glucose-6-phosphate dehydrogenase, and 5'-nucleotidase activity and glycogen. The use of 4-micron sections of methacrylate-embedded tissue allows the evaluation of many more phenotypic markers in serial sections than is currently possible with frozen sections.
D000444:Aldehyde Dehydrogenase / Q000032:analysis*; D000818:Animals; D002273:Carcinogens; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D008113:Liver Neoplasms / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008689:Methacrylates; D011230:Precancerous Conditions / Q000201:enzymology*; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D002273:Carcinogens; D008689:Methacrylates; D005954:Glucosephosphate Dehydrogenase; D000444:Aldehyde Dehydrogenase; D005723:gamma-Glutamyltransferase