title: Primary structure and nuclear localization of a murine homeodomain protein.
abstract: The murine homeobox Hox 1.1 (m6) is the first of a cluster of six boxes on chromosome 6. Using probes and synthetic peptides derived from the Hox 1.1 sequence, we were able to isolate cDNAs and antibodies that allowed us to characterize the product of this homeobox-containing gene. From the open reading frame on the cDNA clone B21, a protein could be predicted, made up of 229 amino acids and having a calculated molecular weight of 25,740. A unique feature of this protein is that it has 15 glutamic acid residues as its carboxyl terminus, which gives it a very hydrophilic and acidic carboxyl terminal structure, most probably folding onto an alpha-helix. A second domain of six amino acids is present on the Hox 1.1 protein, which is conserved in other homeodomain proteins. Antibodies generated against synthetic peptides from the homeobox region were used in the immunoblotting procedure and revealed a major protein band of Mr 31,000 in extracts from 3T3 cells and F9 teratocarcinoma cells induced by retinoic acid and cAMP. The nuclear location of the protein was established by immunofluorescence. The presence of this protein in F9 cell nuclei is in faithful accordance with the kinetics established for the 2.4-kilobase Hox 1.1 transcript during differentiation into parietal endoderm cells.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001221:Aspartate Carbamoyltransferase; D001483:Base Sequence; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing); D004247:DNA / Q000032:analysis; D004080:Dihydroorotase; D005455:Fluorescent Antibody Technique; D005801:Genes, Homeobox; D007163:Immunosorbent Techniques; D051379:Mice; D009097:Multienzyme Complexes; D011506:Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: C033626:CAD trifunctional enzyme; D009097:Multienzyme Complexes; D011506:Proteins; D004247:DNA; D001221:Aspartate Carbamoyltransferase; D004080:Dihydroorotase; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)