Effect of the H2-blocker famotidine on gastric mucosal prostaglandin levels in water immersion stress in rats.
A quantitative and rapid method was developed for determination of tissue prostaglandin (PG) levels using reverse phase high performance liquid chromatography. Using this method, we investigated the effects of famotidine (YM-11170), an H2-blocker, on changes in gastric mucosal PG levels induced by water immersion stress in rats. Gastric mucosal phospholipase (PLase) activity was also estimated. Four kinds of PGs, i.e., 6-keto-PGF1a, PGE2, PGF2a, and PGD2 were detected in gastric mucosa. 6 h water immersion stress induced decreases in all of them at a similar degree, the reduction being about 70% of the control value. Decreases in PLase activity were also observed in rats with 6 h stress. Pretreatment with famotidine prevented decreases in levels of PGs, which are known to have cytoprotective effect, and also maintained PLase activity. These results indicate that famotidine exerts its anti-ulcer action via maintenance of PG levels and PLase activity.
D000818:Animals; D015738:Famotidine; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006635:Histamine H2 Antagonists / Q000494:pharmacology*; D007101:Immersion; D008297:Male; D010740:Phospholipases / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000503:physiopathology; D013315:Stress, Psychological / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology*
D006635:Histamine H2 Antagonists; D011453:Prostaglandins; D013844:Thiazoles; D015738:Famotidine; D010740:Phospholipases