Purification of IMP dehydrogenase from rat hepatoma 3924A.
IMP dehydrogenase (EC 1.1.1.205), the rate-limiting enzyme of de novo GTP biosynthesis and a promising target for cancer chemotherapy, was purified 4860-fold to homogeneity from rat hepatoma 3924A by a method including affinity chromatography in which IMP is bound to epoxy-activated Sepharose 6B. This affinity gel provided a specific elution of the enzyme with 0.5 mM IMP. The final enzyme preparation gave a single band with a molecular weight of 60,000 +/- 1000 on sodium dodecyl sulfate polyacrylamide gel electrophoresis.
D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007168:IMP Dehydrogenase / Q000302:isolation & purification*; D007658:Ketone Oxidoreductases / Q000302:isolation & purification*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008970:Molecular Weight; D051381:Rats
D007168:IMP Dehydrogenase; D007658:Ketone Oxidoreductases