Effect of PGE2 and alpha-adrenergic agonists on AVP-dependent cAMP levels in rabbit and rat CCT.
The effect of prostaglandins and alpha-adrenergic agonists on arginine vasopressin-induced adenosine 3',5'-cyclic monophosphate (cAMP) production was investigated in microdissected rat and rabbit cortical collecting tubules (CCT) incubated in vitro. In rabbit CCT, addition to all media of a prostaglandin synthesis inhibitor increased this production; exogenous prostaglandin E2 (PGE2) induced a reproducible dose-dependent inhibition of cAMP accumulation. Maximal inhibition (mean: 57.5%) was observed with 0.3 microM PGE2, and threshold inhibition was observed with concentrations ranging from 3 to 10 nM PGE2. Inhibition of cAMP levels in rabbit CCT was also obtained with 0.3 microM PGF2 alpha (mean inhibition: 44.3%) but not with alpha-adrenergic agonists studied under the same conditions. The opposite was observed in rat CCT studied in parallel: the alpha-agonists inhibited cAMP production by up to 80%, but PGE2 had no effect.
D000316:Adrenergic alpha-Agonists; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D003000:Clonidine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D015237:Dinoprost; D015232:Dinoprostone; D007685:Kidney Tubules, Collecting; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000316:Adrenergic alpha-Agonists; D011458:Prostaglandins E; D011460:Prostaglandins F; D001127:Arginine Vasopressin; D011433:Propranolol; D015237:Dinoprost; D000242:Cyclic AMP; D015232:Dinoprostone; D003000:Clonidine; D009638:Norepinephrine