title: Circadian rhythms in Neurospora crassa: a clock mutant, prd-1, is altered in membrane fatty acid composition.
abstract: The fatty acid compositions of the phospholipids of Neurospora crassa mutants with altered periods were determined to test the possibility that some of these mutants might have altered membrane composition. In liquid shaker culture in constant light the bd (band) strain, which has a normal period (21.6 h), exhibited a growth-dependent increase in linoleic acid content and a decrease in linolenic acid content during early log phase growth. By late log phase, fatty acid composition was essentially constant. The phospholipid fatty acid compositions of bd strains containing mutations at the frq (frequency) and chr (chrono) loci were indistinguishable from that of the bd strain under the conditions used. However, a bd strain containing a mutation at the prd-1 (period) locus, as well as prd-1 segregants from a cross of this strain to a bd strain, had altered patterns of growth-dependent fatty acid composition; linoleic and linolenic acid contents changed more slow than in the bd strain and continued to change throughout growth. In addition, the fatty acid composition of a bd prd-1 strain on solid medium differed from that of the bd strain. It is proposed that the prd-1 mutation leads to altered membrane homeostasis, which in turn affects circadian rhythmicity because some or all components of the rhythm-generating system are membrane-localized.
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002940:Circadian Rhythm; D005227:Fatty Acids / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D017962:alpha-Linolenic Acid
chemical_list: D005227:Fatty Acids; D008041:Linoleic Acids; D008042:Linolenic Acids; D008563:Membrane Lipids; D010743:Phospholipids; D017962:alpha-Linolenic Acid; D019787:Linoleic Acid