Neural membrane glycoproteins associated with chicken Thy-1: an anti-idiotypic antibody study.
In order to investigate the possible binding of Thy-1 to other neuronal cell surface proteins, anti-idiotypic antibodies were raised using a panel of anti-Thy-1 monoclonal antibodies. Anti-idiotypic antibodies were selected for their ability to bind to day-old chick brain membrane components in enzyme-linked immunosorbent assays (ELISA), and to bind to membrane glycoproteins as determined by Western transfer immunoblotting assays. The 5 monoclonal anti-idiotypic antibodies bind to a membrane glycoprotein component of 70 kDa, and one of the antibodies also binds to 3 higher molecular weight components of 160 kDa, 120 kDa and 90 kDa. These antibodies bind to areas of the chicken cerebellum known to be rich in Thy-1. It is postulated that these molecules are associated with Thy-1, and that the role of Thy-1 on the neuronal cell surface, may be to form complexes with, and/or to stabilise these higher molecular weight glycoproteins during synaptic development.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism*; D002531:Cerebellum / Q000276:immunology / Q000378:metabolism; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005625:Frontal Lobe / Q000276:immunology / Q000378:metabolism*; D007130:Immunoglobulin Idiotypes / Q000378:metabolism*; D007150:Immunohistochemistry; D007518:Isoantibodies / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D007130:Immunoglobulin Idiotypes; D007518:Isoantibodies; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens; C035678:anti-Thy antibody