Immunoneutralization of somatostatin and neurotensin: effect on gastric acid secretion.
Cell lines producing monoclonal antibodies to somatostatin and neurotensin, designated S-10 and NT-C5, respectively, have recently been generated. The purpose of the present immunoneutralization study in urethan-anesthetized gastric fistula rats is 1) to examine the ability of these antibodies to block the inhibitory effect of their target peptides on meal-stimulated gastric acid secretion and 2) to use these antibodies as probes to determine whether somatostatin and/or neurotensin are involved in the inhibition of gastric acid secretion produced by intraduodenal, intra-ileal, and intracolonic fat infusions. The results demonstrate that both S-10 and NT-C5 successfully bound exogenous somatostatin and neurotensin in vivo. S-10 but not NT-C5 prevented the inhibition of gastric acid secretion produced by intraduodenal fat. NT-C5 but not S-10 prevented the inhibition of gastric acid secretion produced by intra-ileal fat. Neither S-10 nor NT-C5 prevented the inhibition of gastric acid secretion produced by intracolonic fat. We conclude that somatostatin is associated with proximal and neurotensin with distal small bowel intestinal fat-induced inhibition of gastric acid secretion.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005502:Food; D005744:Gastric Acid / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009496:Neurotensin / Q000276:immunology / Q000502:physiology*; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D013004:Somatostatin / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D009829:Oleic Acids; D019301:Oleic Acid; D009496:Neurotensin; D013004:Somatostatin