title: Radiation-induced mating-type switching in the yeast Saccharomyces cerevisiae.
abstract: Haploid yeast cells possess two different mating types which are controlled genetically by the MAT locus. Information of the opposite mating type is stored on the same chromosome but not expressed. Radiation may initiate a gene conversion event leading to 'mating-type switching'. This was studied by using X-rays and 254 nm ultraviolet light. X-ray-induced mating type switching shows an oxygen enhancement ratio of 2.9 which is higher than that for survival (1.8) and equals that for double-strand break induction. Mating-type switching by UV is not photoreactivable and depends on a functioning excision repair system. The results are compatible with the interpretation that mating type switching is initiated by a double-strand break in the MAT coding region.
mesh_terms: D005785:Gene Conversion / Q000528:radiation effects*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014965:X-Rays