title: Unimpaired membrane dynamics in parathyroid cells in insulin deficient rats.
abstract: The responsiveness of parathyroid cells in insulin deficient and short-term diabetic rats was investigated by morphometric analysis. Insulin deficiency was produced by intravenous injection of D-mannoheptulose and short-term (7 days) diabetes mellitus by intraperitoneal application of streptozotocin. Parathyroid glands were stimulated for parathyroid hormone secretion by decreasing the serum calcium concentration through intravenous infusion of EGTA. Parathyroid cells of controls, insulin deficient, and short-term diabetic rats responded to reduced serum calcium by a 45% increase of the cell surface area. This increase is assumed to be the result of the membrane-bound transport of parathyroid hormone from the Golgi complex and secretory granules to the plasma membrane and subsequent exocytic release of parathyroid hormone induced by the low serum calcium concentration. Therefore, the unimpaired increase in the cell surface area of parathyroid cells in insulin deficient and short-term diabetic rats indicates that insulin does not modulate the release of parathyroid hormone. It is also considered likely that synthesis of parathyroid hormone is not suppressed in short-term diabetes but that fat metabolism is disturbed leading to accumulation of lipid vacuoles.
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood; D002462:Cell Membrane / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D007328:Insulin / Q000172:deficiency*; D008297:Male; D008854:Microscopy, Electron; D010280:Parathyroid Glands / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D010281:Parathyroid Hormone / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007328:Insulin; D010281:Parathyroid Hormone; D002118:Calcium