NMR analysis and sequence of toxin II from the sea anemone Radianthus paumotensis.
Toxin II from Radianthus paumotensis (RpII) has been investigated by high-resolution NMR and chemical sequencing methods. Resonance assignments have been obtained for this protein by the sequential approach. NMR assignments could not be made consistent with the previously reported primary sequence for this protein, and chemical methods have been used to determine a sequence with which the NMR data are consistent. Analysis of the 2D NOE spectra shows that the protein secondary structure is comprised of two sequences of beta-sheet, probably joined into a distorted continuous sheet, connected by turns and extended loops, without any regular alpha-helical segments. The residues previously implicated in activity in this class of proteins, D8 and R13, occur in a loop region.
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003064:Cnidarian Venoms / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D012615:Sea Anemones; D013820:Thermolysin
D003064:Cnidarian Venoms; D010446:Peptide Fragments; C028800:toxin II (Anemonia sulcata); D013820:Thermolysin