Semi-quantitative immunohistochemical studies on Thy-1 antigen expressed by thymic myoid cells.
Thy-1 antigen expression in the rat thymic myoid cell line R615B2 (Thy-1(+] and R613Ad (Thy-1(-] was studied with a semi-quantitative immunohistochemical assay without disrupting the cell cultures. With this assay, the quantity of Thy-1 antigen on R615B2 cells was detected separately in the cytoplasm and on the cell surface by the use of appropriate fixatives such as chilled ethanol and methanol + 0.3% H2O2. Extracellular Thy-1 antigen was also found in the culture supernatant of R615B2 cells. More than half of extracellular Thy-1 antigen remained in the supernatant even after 100,000 X g centrifugation. No form of Thy-1 antigen was detected at significant levels in R613Ad cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology*; D002460:Cell Line; D003593:Cytoplasm / Q000032:analysis; D005110:Extracellular Space / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D018800:Thy-1 Antigens