title: Molecular cloning and sequence analysis of cDNA encoding human kidney D-amino acid oxidase.
abstract: cDNA clones encoding D-amino acid oxidase were isolated from a human kidney cDNA library by hybridization with cDNA for the pig enzyme. The cDNA insert of 2.0 kilobase pairs long provided coding information for a protein consisting of 347 amino acids. The molecular mass of the enzyme was calculated to be 39,410 Da. The amino acid sequence similarity between the pig and human enzymes is 84.4%, and among the active site residues proposed from chemical modification studies, methionine-110 of the pig enzyme was replaced by threonine. Northern blot analysis confirmed the expression of an mRNA of 2.0 kilobases encoding the D-amino acid oxidase in human kidney.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003605:D-Amino-Acid Oxidase / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D005796:Genes; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine
chemical_list: D004247:DNA; D003605:D-Amino-Acid Oxidase; D004262:DNA Restriction Enzymes