HLA-DQ polymorphism analyzed by sequential restriction endonuclease DNA digestion.
A direct, physical correspondence between certain Pst I-generated genomic DNA fragments and Taq I-generated fragments, revealed with HLA-DQ alpha or -DQ beta gene probes, has been demonstrated. As an immediate consequence, the nature of the DQ and DX hybridized genes contained in the fragments was established. Taq I-generated DQ allelic forms which associate with serologically defined DR1, DR2, and DRw6 specificities were also proven to be sensu stricto "splits" of the Pst I-generated allelic form associated with all three DR specificities.
D000483:Alleles; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D005802:Genes, MHC Class II; D006681:HLA-D Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D006681:HLA-D Antigens; D006683:HLA-DQ Antigens; D004247:DNA; D004262:DNA Restriction Enzymes; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases