DNA sequence and regional assignment of the human follicle-stimulating hormone beta-subunit gene to the short arm of human chromosome 11.
A human genomic DNA fragment in phage lambda containing FSHB, the gene for the beta-subunit of human follicle-stimulating hormone (FSH-beta), was analyzed and the nucleotide sequence of the region of the clone encoding FSH-beta was determined. A subclone of the lambda phage containing 67% of FSH-beta coding sequence was used as hybridization probe to determine the human chromosomal location of FSHB. A panel of mouse-human somatic cell hybrids containing reduced numbers of human chromosomes was screened with the FSHB probe; complete cosegregation of FSHB with human chromosome 11 was observed in all 26 cell hybrids tested. Analysis of a set of cell hybrids containing translocated derivatives of chromosome 11 further localized FSHB to the human chromosome region 11p11.2----11pter. A Hind III restriction fragment length polymorphism (RFLP) detected by another subclone of the lambda phage containing FSHB now provides a genetic marker for this region of the human genome.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D015247:Deoxyribonuclease HindIII; D005640:Follicle Stimulating Hormone / Q000235:genetics*; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length
D004274:DNA, Recombinant; D005819:Genetic Markers; D005640:Follicle Stimulating Hormone; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII