Effect of high hydrostatic pressure on 86Rb+ influx in the erythrocyte of the plaice (Pleuronectes platessa).
1. 86Rb+ influx in the erythrocyte of the plaice (Pleuronectes platessa) has been measured at hydrostatic pressures between 1 and 600 atm at 10 degrees C. 2. The measurements were performed with an experimental medium containing 1% (w/v) bovine serum albumin. In this medium the cells achieved a steady state level of ionic regulation. 3. At normal atmospheric pressure 46% of the 86Rb+ influx was inhibited by furosemide while 42% was inhibited by ouabain, the remainder being inhibited by neither drug. 4. It was found that all three fluxes defined by these drugs were sensitive to pressure. 5. The ouabain sensitive influx was progressively inhibited by increasing pressure, the inhibition at 600 atm being 30%. 6. The furosemide sensitive influx was inhibited by 35% between 100 and 600 atm. 7. In contrast the ouabain + furosemide insensitive influx was doubled by 400 atm. 8. This pattern of pressure inhibition and stimulation resembles that seen in comparable studies in human erythrocytes.
D000818:Animals; D004912:Erythrocytes / Q000378:metabolism*; D005412:Flatfishes / Q000097:blood*; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D007700:Kinetics; D012413:Rubidium / Q000097:blood*; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000097:blood
D012414:Rubidium Radioisotopes; D012964:Sodium; D012413:Rubidium