Effect of 22,25-diazacholesterol dihydrochloride on the spermatogenesis of a wild rat, Bandicota bengalensis.
The effects of 22,25-diazacholesterol dihydrochloride (SC-12937) on the spermatogenesis of the bandicoot rat (Bandicota bengalenesis), heretofore unreported on any mammalian species, were studied. Rats were given a single subcutaneous injection of SC-12937 at a dosage of 100 or 200 mg/kg body weight and were killed on days 2 or 10 post-injection. Quantitative analysis of the germinal epithelium was performed on testicular sections (5 micron) stained with periodic acid-Schiff-hematoxylin to assess spermatogenesis. Treatment with SC-12937 at both dosages was found to interfere with the spermatogenic process of this pest rat. In addition to its antispermatogenic action, at higher dosage (200 mg/kg), this compound produced a marked suppression in accessory organ function. The results indicate that a single administration of SC-12937 in low dosage can cause spermatogenic inhibition without affecting accessory organ function in the bandicoot rat.
D000818:Animals; D001373:Azacosterol / Q000494:pharmacology*; D002784:Cholesterol / Q000031:analogs & derivatives*; D008297:Male; D009115:Muridae / Q000502:physiology*; D013091:Spermatogenesis / Q000187:drug effects*; D013092:Spermatogenesis-Blocking Agents / Q000494:pharmacology*
D013092:Spermatogenesis-Blocking Agents; D002784:Cholesterol; D001373:Azacosterol