Effects of noradrenaline and somatostatin on basal and stimulated mucosal ion transport in the guinea-pig small intestine.
Noradrenaline (NA) and somatostatin (SOM) stimulate intestinal water and ion absorption and are found in mucosal nerve fibres and nerve terminals in submucous ganglia of the guinea-pig small intestine. As the main projection of submucous neurons is to the mucosa, NA and SOM might alter mucosal transport either by a direct effect on the epithelium or indirectly, by affecting submucous neurons. In this study these two possible sites of action of NA and SOM have been investigated in mucosa-submucosa preparations of guinea-pig ileum. In addition, the actions of NA and SOM on the secretory responses caused by stimulation of different populations of submucous neurons have been studied. The stimulants of secretion used were a nicotinic agonist, 1,1-dimethyl-4-phenylpiperazinium (DMPP, 10(-5) M), 5-hydroxytryptamine (5-HT, 10(-7) M) and electrical field stimulation (EFS), which activate cholinergic, noncholinergic and mixed populations of submucous secretomotor neurons, respectively. Segments of intestine were dissected free of external muscle and myenteric plexus and mounted in Ussing chambers. Short-circuit current (Isc) was measured as an indication of net active ion transport across the tissue. NA (greater than or equal to 10(-8) M) and SOM (greater than 10(-10) M) each caused a decrease in Isc, indicating a net increase in ion absorption. The NA response was abolished and the magnitude of the SOM response was reduced to 20% by tetrodotoxin (10(-7) M). DMPP, 5-HT and EFS each stimulated nerves that increased Isc and each of these responses was significantly diminished by NA and SOM.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002217:Carbachol / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology; D013004:Somatostatin / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000494:pharmacology
D012701:Serotonin; D013779:Tetrodotoxin; D013004:Somatostatin; D002217:Carbachol; D009638:Norepinephrine