Kidney extract reversibly inhibits compensatory cell proliferation after unilateral nephrectomy.
Unilateral nephrectomy (uNX) in mice is followed by a transitory increase in cell proliferation in the remaining kidney. To examine whether this response could be related to a negative feedback control of kidney epithelial cell renewal, water extracts were made of kidney homogenate. Five mg freeze-dried extract was injected 18 h post-operatively, and the animals were sacrificed at intervals during the following 54 h. The mitotic rate and the incorporation of tritiated thymidine (3H-TdR) into DNA were measured in the remaining kidney. The results show that the kidney extract reduces both the mitotic rate and the incorporation of 3H-TdR into DNA. In the tubular epithelium in the kidney, the strongest inhibitory effect was found by injecting the extract at 18 or 39 h postoperatively.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D007672:Kidney Cortex / Q000166:cytology* / Q000187:drug effects; D007684:Kidney Tubules / Q000166:cytology* / Q000187:drug effects; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008938:Mitosis; D009392:Nephrectomy; D014020:Tissue Extracts / Q000494:pharmacology*
D014020:Tissue Extracts