Detection of specific antibodies against fimbriae and membrane proteins from the oral anaerobe Bacteroides gingivalis in patients with periodontal diseases.
Sera from a number of patients with periodontal diseases were shown to have specific immunoglobulin G (IgG) antibodies against fimbriae and membrane proteins of Bacteroides gingivalis, a suspected pathogen, by using Western blottin analysis. The sera had a strong tendency to react with fimbriae, or exactly oligomeric structures of fimbriae with a native beta-structure rich-conformation. However, the sera did not react with fimbrilin, a constituent protein of fimbriae, which is denatured by sodium dodecyl sulfate.
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001439:Bacteroides / Q000276:immunology*; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006801:Humans; D007163:Immunosorbent Techniques; D008875:Middle Aged; D008970:Molecular Weight; D010518:Periodontitis / Q000276:immunology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins