Molecular characterization of 125I decay and X-ray-induced HPRT mutants in CHO cells.
The predominant initial lesion induced by 125I decay in DNa is a double-strand DNA break comprising a 'mini deletion' of up to several base pairs (bp). However, Southern analysis of the structure of 125I decay-induced HPRT mutants in Chinese hamster ovary cells revealed that most of the mutants are deletions of thousands of bps. A similar predominance of substantial deletions was also found in X-ray-induced mutants.
D000818:Animals; D002872:Chromosome Deletion; D006224:Cricetinae; D004260:DNA Repair; D005720:Gamma Rays; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007457:Iodine Radioisotopes; D009154:Mutation / Q000528:radiation effects*; D012150:Polymorphism, Restriction Fragment Length; D014965:X-Rays
D007457:Iodine Radioisotopes; D007041:Hypoxanthine Phosphoribosyltransferase