Neuroimmunological electron microscopy with microwave-accelerated fixation.
Immunoelectron microscopy is an important tool used to determine the precise location of immune complexes. Standard concentrations of glutaraldehyde destroy these complexes. This paper describes a method in which the period of glutaraldehyde fixation is shortened by concomitant microwave treatment. Using 1.25% glutaraldehyde and microwave fixation ideal preservation and demonstration of MHC class I antigen on Schwann cells was obtained by the peroxidase method.
D000447:Aldehydes; D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis; D011992:Endosomes / Q000648:ultrastructure; D005404:Fixatives; D005727:Ganglia, Spinal; D005976:Glutaral; D008854:Microscopy, Electron / Q000379:methods*; D008872:Microwaves; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000032:analysis / Q000276:immunology* / Q000648:ultrastructure
D000447:Aldehydes; D000936:Antigen-Antibody Complex; D005404:Fixatives; D005976:Glutaral