The poly(A)-binding protein facilitates in vitro translation of poly(A)-rich mRNA.
To investigate the role of the 73-kDa poly(A)-binding protein in protein synthesis, the effect of the addition of homo-polyribonucleotides on the translation of polyadenylated and non-adenylated mRNA was studied in the rabbit reticulocyte lysate. Poly(A) was found to be the most effective polynucleotide in inhibiting duck-globin mRNA translation, whereas it had no effect on the translation of polyribosomal duck-globin mRNP, or on the endogenous synthesis of the rabbit reticulocyte lysate. The translation of poly(A)-free mRNA was not affected by the addition of poly(A). Furthermore, we found that the inhibiting effect of poly(A) can be reversed by addition of purified poly(A)-binding protein. It is thus likely that the 73-kDa poly(A)-binding protein is an essential factor necessary for poly(A)-rich mRNA translation.
D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D004372:Ducks; D005914:Globins / Q000096:biosynthesis / Q000235:genetics; D066298:In Vitro Techniques; D011061:Poly A / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D039102:Poly(A)-Binding Proteins; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012265:Ribonucleotides / Q000494:pharmacology
D002352:Carrier Proteins; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D012265:Ribonucleotides; D011061:Poly A; D005914:Globins