Putative neurotransmitters in the retinae of three urodele species (Triturus alpestris, Salamandra salamandra, Pleurodeles waltli).
The immunocytochemical localization of several substances with putative neurotransmitter or modulator properties was investigated in the retinae of three urodele species. Gamma-aminobutyric acid-like immunoreactive labelling appeared in different types of amacrine and horizontal cells. In addition, labelled fibres in the optic nerve were detected. It was not possible to determine whether these fibres were ganglion-cell axons or part of an efferent projection. Endogenous serotonin was found in several populations of amacrine cells including stratified and diffuse types. Glucagon-like immunoreactivity appeared in one bistratified amacrine cell type, and neurotensin-like immunoreactivity was detected in a single monostratified amacrine cell type. Metenkephalin-like-immunoreactive labelling was type. Metenkephalin-like-immunoreactive labelling was rare but found in several sublaminae of the inner plexiform layer. Thus each peptide-like-immunoreactive cell type makes up a distinct and unique population of cells and probably has a special functional role in retinal processing. There are striking similarities in the peptide-like immunoreactive patterns of Triturus alpestris and Necturus maculosus whereas in Ambystomatidae the peptide-like-immunoreactive systems appear to be differently organized. This supports the hypothesis that Salamandridae and Proteidae are more closely related to each other than to the Ambystomatidae.
D000818:Animals; D004744:Enkephalin, Methionine / Q000032:analysis / Q000276:immunology; D005934:Glucagon / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry; D009474:Neurons / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D009496:Neurotensin / Q000032:analysis / Q000276:immunology; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000276:immunology; D010999:Pleurodeles / Q000033:anatomy & histology*; D012160:Retina / Q000032:analysis* / Q000166:cytology / Q000276:immunology / Q000294:innervation; D012454:Salamandra / Q000033:anatomy & histology*; D012455:Salamandridae / Q000033:anatomy & histology*; D012701:Serotonin / Q000032:analysis / Q000276:immunology; D014319:Triturus / Q000033:anatomy & histology*; D005680:gamma-Aminobutyric Acid / Q000032:analysis / Q000276:immunology
D018377:Neurotransmitter Agents; D012701:Serotonin; D009496:Neurotensin; D005680:gamma-Aminobutyric Acid; D004744:Enkephalin, Methionine; D005934:Glucagon