Comparative polypeptide analysis of human, murine and strigis herpesviruses with murine cytomegalovirus by polyacrylamide gel electrophoresis.
The polypeptide composition of five purified murine herpesvirus (MHV) strains grown in a stable line of rabbit embryo fibroblasts (REF) was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and compared with herpes simplex virus type 1 (HSV-1). About 24 structural polypeptides of molecular mass ranging from 275,000 to 25,000 were identified in MHV and HSV-1. The polypeptide profiles of MHV and HSV-1, showed a close similarity. The polypeptides of MHV were further compared with those of HSV-1, HSV-2, herpes virus strigis (HVS) and murine cytomegalovirus (MCMV). Differences were found between herpesviruses of different origin and MCMV. SDS-PAGE analysis of the six strains of MCMV labelled with 14C-amino acid hydrolysate also revealed differences in electrophor eticprofiles of MHV and MCMV proteins, what was confirmed by densitometric scanning of HSV-1, MHV and MCMV.
D000818:Animals; D003411:Arvicolinae; D001345:Autoradiography; D001717:Birds; D002460:Cell Line; D003587:Cytomegalovirus / Q000032:analysis*; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts; D006564:Herpesviridae / Q000032:analysis*; D006801:Humans; D009115:Muridae; D010455:Peptides / Q000032:analysis; D018139:Simplexvirus / Q000032:analysis*; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis*
D010455:Peptides; D014764:Viral Proteins