Relationship between RNA polymerase I activity and ornithine decarboxylase in rat liver tissues.
In order to examine the relationship between RNA polymerase I and ornithine decarboxylase (ODC), three lines of experiments were performed, with the following results. The glucocorticoid-induced increase of RNA polymerase I in rat liver nuclei was not abolished by administration of inhibitors of ODC synthesis and activity, namely 1,3-diaminopropane and 2-difluoromethylornithine respectively. Anti-ODC antibody did not cross-react with RNA polymerase I solubilized from rat liver nucleoli, indicating the absence of a common protein sequence in these enzymes. The ODC preparation which was treated with transglutaminase in the presence of putrescine could not stimulate the activity of RNA polymerase I in nuclei of liver and prostate. All these results suggest that the increases in ODC protein or activity are not a prerequisite to the increase in RNA polymerase I after hormonal or physiological stimuli, but rather that the increases in both enzymes are separate responses to the primary stimuli.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology; D003907:Dexamethasone / Q000494:pharmacology; D003959:Diamines / Q000494:pharmacology; D000518:Eflornithine / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D012318:RNA Polymerase I / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011503:Transglutaminases / Q000494:pharmacology
D003959:Diamines; D003907:Dexamethasone; C009475:trimethylenediamine; D011503:Transglutaminases; D012318:RNA Polymerase I; D009955:Ornithine Decarboxylase; D000518:Eflornithine