[A new in vitro model allows use of the same hypothalamus for morphological and functional studies].
A model for combined morphological and functional investigations on the isolated rat mediobasal hypothalamus has been developed. Under these conditions of incubation, the hypothalamic tissue is well preserved, on the basis of photonic and electronic microscopic examinations. This model has been used to study the LHRH system in the rat. LHRH release has been measured in the incubation medium under basal conditions and after KCl-induced depolarisation; the, LHRH has been localized by immunohistochemistry on the hypothalamic fragment.
D000818:Animals; D004594:Electrophysiology; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis / Q000378:metabolism; D007033:Hypothalamus, Middle / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000032:analysis; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis
D007987:Gonadotropin-Releasing Hormone; D013004:Somatostatin; D013973:Thyrotropin-Releasing Hormone; D011189:Potassium Chloride
Un nouveau modèle in vitro permet d'employer le même hypothalamus pour des investigations morphologiques et functionnelles.