Induction of heavy metal binding groups by dexamethasone and Zn in cultured Chinese hamster ovary cells.
Cd binding capacity and pulse polarography were used to study the inducibility of sulfhydryl groups in cultured Chinese hamster ovary cells (wild type and a Cd-resistant mutant) in response to dexamethasone (dex) and Zn. Evidence is presented that both the wild type and the mutant responded to dex and Zn treatment by induction of sulfhydryl groups. In wild type for Zn and dex as well as in the mutant for dex, this induction seems to be in the form of sulfhydryls attached to particulate or membrane fractions in the cells. For Zn in the Cd-resistant mutant the induction was in the form of metallothionein.
D000818:Animals; D002104:Cadmium / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D008667:Metalloproteins / Q000096:biosynthesis*; D008668:Metallothionein / Q000096:biosynthesis*; D009154:Mutation; D010053:Ovary; D011048:Polarography; D013438:Sulfhydryl Compounds / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology*
D008667:Metalloproteins; D013438:Sulfhydryl Compounds; D002104:Cadmium; D003907:Dexamethasone; D008668:Metallothionein; D015032:Zinc