Isolation and characterization of three lethal and hemolytic toxins from the sea anemone Actinia equina L.
Lethal and hemolytic toxins were purified by acetone precipitation, Sephadex G-50, CM-cellulose and CM-Sephadex column chromatography from the tentacles and bodies of the sea anemone Actinia equina. The isolated toxins, with a mol. wt of 19,000 determined by SDS-polyacrylamide gel electrophoresis, differed only slightly in amino acid composition and had a high tryptophan content. The isoelectric points were estimated to be 9.8 for equinatoxin I and 10.5 for equinatoxins II and III. The pure toxins exhibited high lethal potency; the acute i.v. LD50 in mice of equinatoxins I, II and III were 23, 35 and 83 micrograms/kg, respectively. The sigmoidal time course of hemolysis is characteristic of toxins.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003063:Cnidaria / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006460:Hemolysin Proteins / Q000032:analysis / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D012615:Sea Anemones / Q000032:analysis*; D014118:Toxins, Biological / Q000032:analysis / Q000302:isolation & purification*
D000596:Amino Acids; D006460:Hemolysin Proteins; D014118:Toxins, Biological