title: Immunocytochemical localization of glutamic acid decarboxylase (GAD) and glutamine synthetase (GS) in the area postrema of the cat. Light and electron microscopy.
abstract: The present study was designed to investigate the existence of two key enzymes involved in the metabolism of gamma-aminobutyric acid, glutamic acid decarboxylase (GAD) and glutamine synthetase (GS), in the area postrema (AP) of the cat. The results showed that punctuate structures of variable size corresponding to axon terminals, exhibited GAD-immunoreactivity and were distributed in varying densities. The greatest accumulation was present in the caudal and middle segment of the AP and particularly in the area subpostrema, where the aggregation of terminals was extremely dense. The population of the GAD-labelled axon profiles gradually decreased toward the solitary complex. No neuronal bodies were labelled in our preparations. The electron microscopic studies revealed a large variety of contacts between labelled terminals and unlabelled dendrites, axons or neurons. The possibility that the GAD-immunoreactive terminals might correspond to vagal afferent projections was discussed on the basis of our observations and of other studies that employed horseradish peroxidase or degeneration methods. GS-immunoreactivity was seen in ependymoglial cells of the AP, particularly toward the caudal region, and in astrocytes and their processes of the AP proper. The latter were frequently observed around capillaries. The presence of both GAD-immunoreactive profiles and GS-immunostained ependymoglial cells and astrocytes in the AP, provided further immunocytochemical evidence of the functional correlation between the two enzymes.
mesh_terms: D000818:Animals; D002415:Cats; D005968:Glutamate Decarboxylase / Q000032:analysis*; D005974:Glutamate-Ammonia Ligase / Q000032:analysis*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008526:Medulla Oblongata / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000201:enzymology / Q000648:ultrastructure; D009457:Neuroglia / Q000201:enzymology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; D005974:Glutamate-Ammonia Ligase