Isolation and characterization of DNA probes from the short arm of the human X chromosome that detect restriction fragment length polymorphisms.
We have isolated 30 X chromosome specific probes from a flow-sorted library enriched for the human X chromosome. Hybridization to somatic cell hybrids containing different regions of the X chromosome localized nine of these probes to Xp. After testing 185 probe-enzyme combinations, three of the Xp probes were found to detect restriction fragment length polymorphisms.
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014960:X Chromosome
D004247:DNA; D004262:DNA Restriction Enzymes