title: A comparative study of high affinity choline uptake and choline utilization in cholinergic and non-cholinergic tissues.
abstract: Comparative studies of [3H]choline accumulation were done in the Limulus corpora pedunculata, abdominal ganglia and cardiac ganglion. Dual uptake processes for choline were found in all three tissues. In acute experiments, the corpora pedunculata high affinity choline uptake system showed exclusive sensitivity to ouabain. Prolonged exposure to ouabain revealed that the HAChUS of all three tissues were significantly inhibited. The metabolism of [3H]choline transported via the high affinity process in the three tissues was studied. [3H]Acetylcholine was a major product of the [3H]choline taken up by the corpora pedunculata and the abdominal ganglia. Phosphorylcholine was the major product seen in cardiac ganglion extracts and occurred in significant proportions in abdominal ganglia extracts. [3H]Acetylcholine was not detected in cardiac ganglion extracts. Treatment with either lithium chloride or hemicholinium-3 markedly inhibited high affinity uptake of [3H]choline in all three tissues.
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects; D002794:Choline / Q000378:metabolism*; D005260:Female; D005724:Ganglia / Q000378:metabolism; D006426:Hemicholinium 3 / Q000494:pharmacology; D006737:Horseshoe Crabs / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D010275:Parasympathetic Nervous System / Q000378:metabolism*
chemical_list: D006426:Hemicholinium 3; D010042:Ouabain; D008094:Lithium; D002794:Choline