Comparison of thyroid stimulating activities measured by cyclic AMP production, those by radioiodine uptake in FRTL-5 cells and TSH-binding inhibitory activities in patients with hyperthyroid and euthyroid Graves' diseases.
By using an assay measuring cAMP production in FRTL-5 thyroid cells, thyroid stimulating antibodies (TSab) were detected in all of 15 patients with euthyroid Graves' disease (EG) and of 26 patients with hyperthyroid Graves' disease (HG). There was no significant difference between TSab activities in EG and in HG. In an effort to elucidate why EG patients remain euthyroid in spite of having TSab, we investigated the effect of the patient's crude immunoglobulin fractions on 125I uptake in FRTL-5 thyroid cells, one of the indices of stimulation subsequent to cAMP production. The 125I uptake stimulating (IUS) activity was positive in 46.7% (7/15) of EG patients and 88.5% (23/26) of HG patients, being significantly lower in the former than in the latter (P less than 0.02). Although the IUS activities significantly correlated with TSab activities in 41 patients with EG and HG (r = 0.784, P less than 0.001), the ratio of IUS to TSab in EG tended to be lower than that in HG. TSH-binding inhibitor immunoglobulins (TBII) activities in EG patients were negative or weakly positive, being significantly lower than those in HG patients (P less than 0.001). Thus, the ratios of TBII to both TSab and IUS activities were significantly higher in HG than in EG (P less than 0.01, P less than 0.001, respectively). The in vitro IUS activities also correlated with TBII activities (r = 0.441, P less than 0.001) and in vivo 99mTc thyroid uptake (r = 0.401, P less than 0.001) in both EG and HG patients.(ABSTRACT TRUNCATED AT 250 WORDS)
D000328:Adult; D002460:Cell Line; D000242:Cyclic AMP / Q000096:biosynthesis*; D005260:Female; D006111:Graves Disease / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006980:Hyperthyroidism / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism*; D018828:Immunoglobulins, Thyroid-Stimulating; D007457:Iodine Radioisotopes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011989:Receptors, Thyrotropin / Q000378:metabolism*; D013667:Technetium / Q000378:metabolism; D013961:Thyroid Gland / Q000276:immunology*
D007074:Immunoglobulin G; D018828:Immunoglobulins, Thyroid-Stimulating; D007457:Iodine Radioisotopes; D011989:Receptors, Thyrotropin; D013667:Technetium; D000242:Cyclic AMP