Effects of pancreatic acinar cell surface antibodies and complement on isolated rat acinar cells in vitro.
Surface directed pancreatic acinar cell antibodies raised by immunization of rabbits with suspensions of viable isolated rat acinar cells were utilized to study immune cytolytic processes as a model of in vitro pancreatic injury. The antibodies produced were bound to rat pancreatic acinar cell surface determinants and significantly damaged freshly separated acinar cells by immune cytolytic mechanisms. Addition of complement accelerated the cytolytic effects on the target cells in a dose-dependent manner. The decline of acinar cells was dependent only on the presence of the immune cytolytic potential and not on the number of already damaged cells. Morphologic changes in the cells induced by the agents applied were revealed by both transmission and scanning electron microscopy. The presented experimental model seems a valuable tool for further investigations at the cellular level into the contribution of primarily occurring acinar cell injury in triggering the subsequent pathophysiological mechanisms initiating autodigestion of the pancreatic gland in the pathogenesis of acute pancreatitis.
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000494:pharmacology; D003165:Complement System Proteins / Q000276:immunology* / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D008565:Membrane Proteins / Q000276:immunology* / Q000494:pharmacology; D010179:Pancreas / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D013861:Thiocyanates
D000906:Antibodies; D005452:Fluoresceins; D008565:Membrane Proteins; D013861:Thiocyanates; D003165:Complement System Proteins; D016650:Fluorescein-5-isothiocyanate