Hormones with adrenocorticotropic and opiate-like activities from the carp (Cyprinus carpio) pituitary.
Carp (Cyprinus carpio) pituitary acetone powder was extracted with a mixture of water, hydrochloric acid and acetone. An acid acetone powder was formed by adding the pituitary extract into a large volume of chilled acetone and subsequently recovering the precipitate. The powder was subjected to ion exchange chromatography on CM cellulose. Fractions adsorbed on the ion exchanger exhibited ACTH-like activity as evidenced in the ability to stimulate lipolysis in isolated hamster adipocytes and corticosterone production in isolated rat adrenal decapsular cells and also in cross-reactivity in an ACTH-specific radioimmunoassay. A portion of the ACTH-like bioactivity and immunoactivity was unadsorbed on the ion exchanger. Opiate-like activity in opiate receptor binding assay, employing [3H]D-ala2-D-leu5 enkephalin or [3H]naloxone as ligand, also resided in fractions adsorbed on CM cellulose. The data indicate a separation of ACTH-like and opiate-like activities, and the presence of opiate-like molecules with different affinities of binding to mu and delta opiate receptors.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism; D002347:Carps; D003345:Corticosterone / Q000378:metabolism; D006224:Cricetinae; D004723:Endorphins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D009270:Naloxone / Q000378:metabolism; D010902:Pituitary Gland / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000187:drug effects / Q000378:metabolism
D004723:Endorphins; D011957:Receptors, Opioid; D009270:Naloxone; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone