Heterogeneity of GABAergic cells in cat visual cortex.
Antibodies against neuropeptides and against a vitamin D-dependent calcium-binding protein (CaBP) label small cells with nonpyramidal-like morphology in the cat visual cortex (areas 17, 18, and 19). Since GABAergic cells are interneurons, a double-staining procedure was used to test for the coexistence of cholecystokinin (CCK), somatostatin (SRIF), neuropeptide Y (NPY), corticotropin-releasing factor (CRF), vasoactive intestinal polypeptide (VIP), and CaBP with glutamic acid decarboxylase (GAD). Our results show that CRF and VIP do not coexist with GAD, while the 3 other peptides and CaBP do. Hence GAD-positive cells can be subdivided into 4 broad groups: (1) cells that are only GAD-positive, (2) cells that are GAD- and CaBP-positive, (3) GAD-positive neurons also containing CCK, and (4) GAD-positive cells that also contain SRIF. A small subset of class 2 also contains SRIF and most cells of class 4 also contain NPY. The 4 classes of GAD-positive cells differ in laminar position: class 1 predominates in layers IV and V, classes 2 and 3 in the upper laminae (II and III), and class 4 in the deepest layer (VI).
D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis; D002415:Cats; D002766:Cholecystokinin / Q000032:analysis; D003346:Corticotropin-Releasing Hormone / Q000032:analysis; D005968:Glutamate Decarboxylase / Q000032:analysis; D007150:Immunohistochemistry; D009478:Neuropeptide Y / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis; D014793:Visual Cortex / Q000032:analysis / Q000166:cytology*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D002135:Calcium-Binding Proteins; D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid; D002766:Cholecystokinin; D003346:Corticotropin-Releasing Hormone; D005968:Glutamate Decarboxylase