title: Electron microscopic analysis of in vitro interaction of Rickettsia prowazekii with guinea pig macrophages. II. Macrophages from immune animals.
abstract: Alike to macrophages from intact animals, reproduction, destruction and formation of spheroplast-like forms were observed in macrophages from immune guinea pigs 2 months post-infection (p.i.) with the virulent Breinl strain of Rickettsia prowazekii. Unlike to the former, immune macrophages revealed phagolysosomes which were larger in size and contained more rickettsiae showing morphologic signs of destruction. Spheroplast-like forms occurred more often and were more numerous than in intact animals. Structures morphologically similar to L-forms of gram-negative bacteria and that of chlamydiae were also detected. After adding immune serum, more intact rickettsiae and spheroplasts were found in phagosomes as well as more phagolysosomes contained rickettsiae and spheroplasts with morphologic signs of destruction. It is suggested that clearance of immune macrophages from rickettsiae is mediated by at least two processes: on one hand by destruction of rod-shaped rickettsiae within phagolysosomes and, on the other hand, by formation and subsequent destruction of spheroplast-like forms within vacuoles, which probably also function as phagolysosomes.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D006168:Guinea Pigs; D007106:Immune Sera; D008264:Macrophages / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010588:Phagosomes / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure; D012283:Rickettsia prowazekii / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D013104:Spheroplasts / Q000648:ultrastructure; D014438:Typhus, Epidemic Louse-Borne / Q000276:immunology
chemical_list: D007106:Immune Sera