Tyrosine hydroxylase and monoamine oxidase-A activity increases in differentiating human neuroblastoma after elimination of dividing cells.
SY5Y neuroblastoma cells were treated with retinoic acid to induce differentiation of neuroblast-like cells and with aphidicolin (an inhibitor of DNA polymerase) to eliminate the flat cells present in the long-term cultures and masking some of the biochemical developmental changes. Catecholaminergic enzyme (tyrosine hydroxylase and monoamine oxidase-A) activity was consistently increased with development and the increase was significantly greater after aphidicolin-induced elimination of dividing, non-neuronal cells.
D000818:Animals; D016590:Aphidicolin; D002454:Cell Differentiation; D002460:Cell Line; D002999:Clone Cells; D004224:Diterpenes / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism*; D008959:Models, Neurological; D008995:Monoamine Oxidase / Q000378:metabolism*; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology*; D014212:Tretinoin / Q000494:pharmacology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D004224:Diterpenes; D007527:Isoenzymes; D016590:Aphidicolin; D014212:Tretinoin; D014446:Tyrosine 3-Monooxygenase; D008995:Monoamine Oxidase