title: Cytogenetic and isozyme profile of Sabethes cyaneus: a mosquito of the neotropical canopy.
abstract: Sabethes cyaneus, a newly colonized culicine mosquito from the Panamanian forest canopy, has a distribution range from Honduras to Argentina. Cytogenetic studies, the first on any Sabethes species, revealed a karyotype of three pairs of homomorphic chromosomes (2n = 6). Well-developed polytene chromosomes were discovered in the larval salivary glands and a photomap standard was constructed. Clear banding patterns and consistent landmarks distinguished each of the six arms. Substantial asynapsis occurred in the three polytene chromosomes, although the banding pattern of the homologous regions appeared homosequential. A nucleolar organizer was localized in region 5A of the shortest chromosome by the recurrent association of this region with the nucleolus. The large band in region 5A was found heterozygous for width and a deletion. Additional, less conspicuous, polymorphisms for band width and staining intensity were distributed throughout the genome. Biochemical studies of 31 enzyme loci revealed 10 loci to be polymorphic, with an average heterozygosity of 13 percent. Differential expression in developmental stages occurred for 11 loci involving six enzymes.
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D009033:Culicidae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D007527:Isoenzymes / Q000235:genetics*; D007621:Karyotyping; D008677:Metaphase
chemical_list: D007527:Isoenzymes