Maturation of bovine oocytes transplanted into bovine follicles of follicular and luteal phase.
Forty-one bovine host follicles with guest oocytes were studied. Fifty of these host follicles from the follicular phase and 26 follicles from the luteal phase of the cycle were cultured in a continuous flux system. No hormones were added to the follicular fluid or the culture medium. After culturing there was no close contact between the oocyte complex and the follicular wall. Of the oocytes cultured in the follicular-phase follicles, 27.2% remained in the germinal vesicle stage and 7.1% reached MII, whereas 9.9% of the oocytes cultured in the luteal-phase follicles remained in germinal vesicle phase and 29.5% reached MII. Of the oocytes cultured in the luteal-phase host follicles, 15.2% were fertilized in vitro; none of the oocytes cultured in follicular-phase follicles were fertilized.
D000818:Animals; D002417:Cattle; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D005498:Follicular Phase; D008183:Luteal Phase; D009865:Oocytes / Q000502:physiology / Q000637:transplantation*; D006080:Ovarian Follicle / Q000502:physiology*