title: Characterization of the glutamyl-tRNA(Gln)-to-glutaminyl-tRNA(Gln) amidotransferase reaction of Bacillus subtilis.
abstract: In Bacillus subtilis, the formation of glutaminyl-tRNA is accomplished by first charging tRNA(Gln) with glutamate, which is then amidated. Glutamine was preferred over asparagine and ammonia as the amide donor in vitro. There is a functional analogy of this reaction to that catalyzed by glutamine synthetase. Homogeneous glutamine synthetase, from either B. subtilis or Escherichia coli, catalyzed the amidotransferase reaction but only about 3 to 5% as well as a partially purified preparation from B. subtilis. Several classes of glutamine synthetase mutants of B. subtilis, however, were unaltered in the amidotransferase reaction. In addition, there was no inhibition by inhibitors of either glutamine synthetase or other amidotransferases. A unique, rather labile activity seems to be required for this reaction.
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001216:Asparagine / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D003980:Diazooxonorleucine / Q000494:pharmacology; D005974:Glutamate-Ammonia Ligase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D006358:Hot Temperature; D008717:Methionine Sulfoximine / Q000494:pharmacology; D009154:Mutation; D019884:Nitrogenous Group Transferases; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012351:RNA, Transfer, Gln / Q000378:metabolism; D014166:Transferases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D012346:RNA, Transfer, Amino Acyl; D012351:RNA, Transfer, Gln; D003980:Diazooxonorleucine; D005973:Glutamine; D008717:Methionine Sulfoximine; D001216:Asparagine; D000641:Ammonia; D014166:Transferases; D019884:Nitrogenous Group Transferases; C047694:glutamyl-tRNA(Gln) amidotransferase; D005974:Glutamate-Ammonia Ligase