Transmembrane proton-motive potential of Spiroplasma floricola.
In Spiroplasma floricola, the transmembrane proton-motive potential delta p was studied. It is composed of a transmembrane electric potential difference, delta psi, and a transmembrane proton gradient, delta pH, according to delta p = delta psi - (Z.delta pH). Using a potential-sensitive carbocyanine dye and 5,5'-dimethyl[2-14C]oxazolidine-2,4-dione as probes, delta psi and delta pH were measured at different [H+] of the medium, and delta p was calculated to be remarkably constant at -123 mV +/- 16% over a wide range of external pH values. Inhibition experiments indicated that it is generated by a membrane-bound, electrogenic, proton-translocating ATPase.
D001426:Bacterial Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D006180:Proton-Translocating ATPases / Q000378:metabolism; D011522:Protons; D013149:Spiroplasma / Q000201:enzymology / Q000502:physiology*
D001426:Bacterial Proteins; D011522:Protons; D006180:Proton-Translocating ATPases