Effects of potassium and norepinephrine on calcium influx in guinea-pig vas deferens.
The effects of potassium (K+) and adrenoceptor agonists on 45Ca influx and contraction of isolated vas deferens from reserpine-treated guinea-pigs has been investigated. K+ (18-84 mM) increased 45Ca influx with the maximum influx occurring at 50 mM K+. The rate of influx upon exposure to K+ was highest in the first 2 min and decreased thereafter. In contrast to K+, norepinephrine, methoxamine and xylazine did not increase the rate of 45Ca influx. Norepinephrine and methoxamine, but not xylazine, inhibited 45Ca influx induced by high K+. The inhibitory effect of norepinephrine was also present in denervated tissues. Prazosin, but not yohimbine or propranolol, blocked the inhibitory effect of norepinephrine. Potassium-induced contractions were significantly potentiated in the presence of norepinephrine, especially at low calcium concentrations. We conclude that K+ contracts the guinea-pig vas deferens by increasing the influx of calcium whereas stimulation of alpha 1-adrenoceptors prevents 45Ca influx elicited by high potassium.
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008729:Methoxamine / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology*; D014649:Vas Deferens / Q000187:drug effects / Q000378:metabolism; D014991:Xylazine / Q000494:pharmacology
D000316:Adrenergic alpha-Agonists; D002132:Calcium Radioisotopes; D014991:Xylazine; D008729:Methoxamine; D011188:Potassium; D002118:Calcium; D009638:Norepinephrine