title: Effects of adenylate cyclase toxin from Bordetella pertussis on human neutrophil interactions with Coccidioides immitis and Staphylococcus aureus.
abstract: Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by luminol-enhanced chemiluminescence and superoxide release. The adenylate cyclase toxin-containing extract blocked neutrophil-mediated inhibition of N-acetylglucosamine incorporation by arthroconidia of Coccidioides immitis in a dose-dependent fashion but had no effect on neutrophil phagocytosis of Candida glabrata and only a slight inhibitory effect on arthroconidial attachment. Neither purified pertussis toxin nor extracts from Bordetella mutants lacking the adenylate cyclase toxin affected neutrophil-mediated inhibition of arthroconidial N-acetylglucosamine incorporation. These studies indicate that adenylate cyclase toxin, alone or in concert with other B. pertussis-elaborated toxins, blocks neutrophil inhibition of arthroconidia, primarily by affecting neutrophil responses other than attachment or phagocytosis.
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000378:metabolism*; D001427:Bacterial Toxins / Q000494:pharmacology*; D001770:Blood Bactericidal Activity / Q000187:drug effects; D001886:Bordetella pertussis / Q000201:enzymology*; D003045:Coccidioides / Q000276:immunology; D000242:Cyclic AMP / Q000096:biosynthesis; D006801:Humans; D009504:Neutrophils / Q000502:physiology*; D010101:Oxygen Consumption; D037342:Pertussis Toxin; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D037361:Adenylate Cyclase Toxin; D001427:Bacterial Toxins; D010566:Virulence Factors, Bordetella; D013481:Superoxides; D000242:Cyclic AMP; D037342:Pertussis Toxin; D000262:Adenylyl Cyclases; D000117:Acetylglucosamine