title: Prospective study of radioimmunoassay for antibodies against neutrophil cytoplasm in diagnosis of systemic vasculitis.
abstract: The diagnosis and management of Wegener's granulomatosis and microscopic polyarteritis are complicated by the lack of specific diagnostic tests. The diagnostic performance of a solid-phase radioimmunoassay, which detects the autoantibodies against neutrophil cytoplasm present in these disorders, was assessed in a prospective study of patients with suspected vasculitis and/or rapidly progressive nephritis. The assay had a sensitivity and specificity of 96% when carried out in combination with a specific inhibition stage and indirect immunofluorescence staining of alcohol-fixed normal neutrophils.
mesh_terms: D001323:Autoantibodies / Q000032:analysis*; D003593:Cytoplasm / Q000276:immunology; D014890:Granulomatosis with Polyangiitis / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D010488:Polyarteritis Nodosa / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy; D011446:Prospective Studies; D011863:Radioimmunoassay
chemical_list: D001323:Autoantibodies