Insertion and truncation of c-myb by murine leukemia virus in a myeloid cell line derived from cultures of normal hematopoietic cells.
A retroviral insertion into the c-myb gene, which resulted in a 3' truncation, was found in an in vitro-derived myeloid cell line. The retroviral insertion occurred at precisely the same nucleotide at which another murine leukemia virus insertion occurred in an in vivo-induced myeloid leukemia. These findings suggest that comparable events may be required for the derivation of myeloid cell lines in vitro and for induction of myeloid leukemia in vivo.
D001483:Base Sequence; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000032:analysis / Q000382:microbiology / Q000473:pathology; D006378:Helper Viruses / Q000302:isolation & purification; D006412:Hematopoietic Stem Cells / Q000032:analysis / Q000382:microbiology / Q000473:pathology*; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000502:physiology*; D008979:Moloney murine leukemia virus / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020598:Proto-Oncogene Proteins c-myb; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic
D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger