The distribution of cholinergic enzymes and 3H-QNB binding in smooth muscles of the cow stomach (Bos taurus).
1. The activity of choline acetyltransferase (CAT) and cholinesterases (ChEs), and the binding of 3H-quinuclidinyl benzilate (3H-QNB) were measured in the longitudinal and circular muscle layers from the four compartments of the cow stomach. 2. CAT and ChEs activity were unevenly distributed in the various regions but distributional pattern of activity of both enzymes was very similar. 3. The maximum binding sites of 3H-QNB were also unequal in the various regions and the distribution of 3H-QNB binding was also similar to that of the activity of two enzymes. 4. These results suggest that cholinergic fibers and muscarinic receptors are not distributed symmetrically throughout the smooth muscle layers of the four compartments of the cow stomach. There may be a positive correlation between the innervation and the density of the receptors on the smooth muscle.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002794:Choline / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002799:Cholinergic Fibers / Q000378:metabolism; D002802:Cholinesterases / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D009130:Muscle, Smooth / Q000294:innervation / Q000378:metabolism*; D011812:Quinuclidines / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism; D013270:Stomach / Q000294:innervation; D014018:Tissue Distribution
D011812:Quinuclidines; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; D002802:Cholinesterases; D002794:Choline