title: Evolution of the sensitivity of isolated adipocytes of ewes to the antilipolytic action of adenosine during pregnancy and lactation.
abstract: Four successive biopsies of omental adipose tissue were performed on the 43rd, 100th, 140th days of pregnancy and during the 3rd week of lactation in 6 "Pré-Alpes" ewes. Using incubated isolated adipocytes, we studied the evolution of the antilipolytic effect of adenosine. Adenosine presented a significant inhibitory effect on basal lipolysis only at the end of pregnancy. The antilipolytic effect of adenosine on the stimulated lipolysis by a beta-agonist (isoproterenol 400 nM) increased during pregnancy. The maximal effect was observed 1 week before parturition. The level of inhibition remained high during lactation. The lipolytic effect of adenosine-deaminase was enhanced during pregnancy and lactation and evolved similar to the observed action of adenosine. Results suggest that the antilipolytic action of adenosine may have a physiologic importance.
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D007774:Lactation; D008066:Lipolysis / Q000187:drug effects*; D011247:Pregnancy; D012756:Sheep
chemical_list: D000241:Adenosine