Development and preliminary evaluation of a slide latex agglutination assay for detection of Clostridium perfringens type A enterotoxin.
A slide latex agglutination (SLA) assay was developed for rapid screening for Clostridium perfringens type A enterotoxin (CPE). SLA specifically detected CPE added to buffer or normal feces (sensitivity limit of 1 microgram CPE/g feces). Using clinical fecal samples from C. perfringens food poisoning cases, a strong correlation was shown between SLA results and results from other CPE assays and between SLA results and illness status.
D003016:Clostridium perfringens; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000032:analysis; D005517:Foodborne Diseases / Q000175:diagnosis; D006801:Humans; D007841:Latex Fixation Tests
D004768:Enterotoxins; C035598:enterotoxin, Clostridium