Analysis of electrophoretypes of rotavirus from diarrhoeic faeces of neonatal buffalo calves in India.
Two distinct rotavirus RNA electrophoretypes were revealed by polyacrylamide gel electrophoresis followed by silver staining (PAGE-SS). The rotavirus was sampled from the faeces of buffalo calves in India from October, 1986 to January, 1987. In the buffalo farm under study one electrophoretype was prevalent first and then the second appeared after the disappearance of the first electrophoretype. For the detection of rotavirus in the diarrhoeic faeces of buffalo calves PAGE-SS was found more sensitive when compared with agar gel immunodiffusion and discontinuous counter immunoelectrophoresis tests.
D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology; D002020:Buffaloes / Q000382:microbiology*; D003378:Counterimmunoelectrophoresis; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D005779:Immunodiffusion; D007194:India; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000382:microbiology / Q000662:veterinary*
D012367:RNA, Viral