P-fimbriae receptors in patients with chronic pyelonephritis.
The adherence of fluorescein isothiocyanate-labeled P-fimbriated Escherichia coli to uroepithelial cells from 19 women with chronic pyelonephritis was determined with the fluorescence-activated cell sorting technique. The application of this method has made it possible to study bacterial binding to a large number of cells. Renal function was determined in all patients and the recurrences of P-fimbriated Escherichia coli bacteriuria, cystitis and acute pyelonephritis during a 3-year followup were studied. We found a significant correlation between the P-fimbriae receptor accessibility on uroepithelial cells and glomerular filtration rate (r equals -0.75, p less than 0.001). Uroepithelial cells from the patients with chronic pyelonephritis and renal insufficiency had a higher binding capacity of P-fimbriated Escherichia coli than uroepithelial cells from patients with a normal glomerular filtration rate. There was no correlation between kidney function and the availability of P-fimbriae receptors in a control group of patients with polycystic kidney disease.
D000328:Adult; D001422:Bacterial Adhesion; D002469:Cell Separation; D004848:Epithelium / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D004927:Escherichia coli Infections / Q000276:immunology* / Q000503:physiopathology; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology*; D005434:Flow Cytometry; D005919:Glomerular Filtration Rate; D006801:Humans; D007668:Kidney / Q000503:physiopathology; D008875:Middle Aged; D011704:Pyelonephritis / Q000276:immunology* / Q000503:physiopathology; D011971:Receptors, Immunologic / Q000276:immunology*
D011971:Receptors, Immunologic; C037872:pili, bacterial receptor