title: Increase in K+-stimulated, Ca2+-dependent release of [3H]glutamate from rat dentate gyrus three days after induction of long-term potentiation.
abstract: Long-term potentiation (LTP) was induced by unilateral tetanic stimulation of the perforant path in the dentate gyrus of rats with implanted electrodes. Evoked potentials were monitored for the subsequent 3 days in one group, and for 23 days in another. The dentate gyrus was removed bilaterally and slices prepared and stored in 10% DMSO/Krebs for subsequent analysis of K+-stimulated, Ca2+-dependent release of [3H]glutamate. In the 3-day group, in which the mean increase in the population EPSP was 35% at the time of sacrifice, release from the tetanized side was significantly greater than from the unstimulated side. In the 23-day group, both the increase in the EPSP and the increase in release from the tetanized side had declined to statistically insignificant levels. These results extend to a period of several days the previously observed association between LTP and increased K+-stimulated, Ca2+-dependent release of transmitter.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004558:Electric Stimulation; D004594:Electrophysiology; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006624:Hippocampus / Q000378:metabolism* / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013997:Time Factors; D014316:Tritium
chemical_list: D005971:Glutamates; D014316:Tritium; D018698:Glutamic Acid; D011188:Potassium; D002118:Calcium