[Mitogenic action of the liquid phase of a microbial suspension of Bordetella pertussis].
The suspension of B. pertussis cells in 0.15 M NaC1 solution, used for the preparation of corpuscular pertussis vaccine contains components loosely bound to microbial cells and producing pronounced mitogenic effect on mouse splenocytes at a concentration of 10 micrograms/ml. The mitogenic activity of B. pertussis is due to complex substances (lipopolysaccharide, protein, nucleic acids) with a wide range of molecular weights (70,000 to greater than 400,000). The mitogenic factor showing no leukocyte-stimulating and protective activity has been isolated by sedimentation with ammonium sulfate and gel filtration on Sephadex G-200. The mitogenic activity of B. pertussis lipopolysaccharide in the blast transformation test has been confirmed.
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology / Q000494:pharmacology; D001886:Bordetella pertussis / Q000276:immunology / Q000472:pathogenicity*; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D008934:Mitogens; D008970:Molecular Weight; D013535:Suspensions
D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D008934:Mitogens; D013535:Suspensions
Mitogennaia aktivnost' zhidkoĭ fazy mikrobnoĭ vzvesi Bordetella pertussis.