Atypical acinar cell lesions of the pancreas in mice induced by 4-hydroxyaminoquinoline-1-oxide.
The carcinogenic effect of 4-hydroxyaminoquinoline-1-oxide (4-HAQO) on exocrine pancreas of mice after a single intravenous (i.v.) injection was investigated. At a dose of 24 mg/kg body weight, 100% of the mice killed at the end of 40 weeks had developed atypical acinar cell foci (AAF) in the pancreas. Each pancreas contained an average of 18.3 +/- 2.7 AAF. Cells in AAF were arranged as acini and their cytoplasm contained eosinophilic zymogen granules. The nuclei were large, round to oval, basally located and contained 1-2 prominent nucleoli. Cells in AAF showed a markedly increased mitotic activity with an index of 4 +/- 0.7/1000 cells as compared to a value of 0.25 +/- 0.25 in the acinar cell population of control pancreas. Autoradiographic studies showed a labeling index of 23 +/- 3 in AAF as compared to only 1.5 +/- 0.29 in the pancreatic acinar cells of controls. These findings indicate that 4-HAQO induces pancreatic lesions in mice which are quite similar to the acidophilic foci observed in rat pancreas and may serve as another useful model for studies of pancreatic carcinogenesis.
D015108:4-Hydroxyaminoquinoline-1-oxide / Q000633:toxicity*; D000634:Aminoquinolines / Q000633:toxicity*; D000818:Animals; D002467:Cell Nucleus / Q000473:pathology; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D010182:Pancreatic Diseases / Q000139:chemically induced* / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000032:analysis
D000634:Aminoquinolines; D015108:4-Hydroxyaminoquinoline-1-oxide; D005723:gamma-Glutamyltransferase