The mdr1 gene, responsible for multidrug-resistance, codes for P-glycoprotein.
The development of simultaneous resistance to multiple drugs in cultured cells occurs after selection for resistance to single agents. This multidrug-resistance phenotype is thought to mimic multidrug-resistance in human tumors treated with chemotherapy. Both the expression of a membrane protein, termed P170 or P-glycoprotein, and the expression of a cloned DNA fragment, termed mdr1, have been shown independently to be associated with multidrug-resistance in cultured cells. In this work, we show that human KB carcinoma cells which express the mdr1 gene also express P-glycoprotein, and that cDNAs encoding P-glycoprotein cross-hybridize with mdr1 cDNAs. Thus, the mdr1 gene codes for P-glycoprotein.
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002460:Cell Line; D003078:Colchicine; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D003609:Dactinomycin; D004317:Doxorubicin; D004351:Drug Resistance / Q000235:genetics*; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D009693:Nucleic Acid Hybridization
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004274:DNA, Recombinant; D006023:Glycoproteins; D003609:Dactinomycin; D004317:Doxorubicin; D004247:DNA; D003078:Colchicine