Evolution of neurotransmitter-related markers in the vertebrate telencephalon. Comparative microchemical study in discrete brain regions of a frog and a turtle.
1. Neurochemical markers related to cholinergic, GABAergic and glutamatergic/aspartatergic neurotransmission have been measured in telencephalic areas obtained by microdissection from frog (Rana esculenta) and turtle (Pseudemys scripta elegans) brain. 2. In both species, pallial areas showed remarkably higher levels of synaptosomal D-3H-aspartate high affinity uptake than basal regions. Conversely, striatal and septal areas possessed higher levels of the GABAergic marker glutamate decarboxylase (GAD) than the pallium. 3. A differential distribution of GAD was noticed in striatal regions, highest levels of the enzyme being present in the ventral striatum, followed by the nucleus accumbens and the dorsal striatum. 4. Cholinergic markers choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) were rather uniformly distributed in the frog telencephalon, while, in the turtle, cholinergic markers were several-fold higher in the basal telencephalon, particularly in the striatum, than in the pallium. 5. The turtle dorsal ventricular ridge possessed ChAT levels more similar to the striatal than to the cortical ones. On the contrary, D-3H-aspartate uptake in the dorsal ventricular ridge was close to the highest levels found in cortical areas. 6. The quantitative neurochemical approach adopted for the present study appears to be a useful tool to investigate the problem of homologies and to gain new information on the evolution of neuron populations and neuronal connections in the vertebrate telencephalon.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism / Q000502:physiology; D005075:Biological Evolution; D001923:Brain Chemistry; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism / Q000502:physiology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010275:Parasympathetic Nervous System / Q000378:metabolism; D011893:Rana esculenta; D013687:Telencephalon / Q000033:anatomy & histology / Q000378:metabolism*; D014426:Turtles / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000502:physiology
D005971:Glutamates; D018377:Neurotransmitter Agents; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; D005968:Glutamate Decarboxylase