A zebrafish homeobox-containing gene with embryonic transcription.
A genomic library of zebrafish (Brachydanio rerio) was constructed and screened with homeobox-containing probes. One of the strongly cross-hybridizing clones was characterized by DNA sequencing. The deduced amino acid sequence exhibits extensive homology (greater than 80%) relative to the Antennapedia-class of Drosophila homeobox sequences. Characterization of the gene with respect to expression demonstrated that two transcripts of 2.1 and 1.4 kb, respectively, are present in embryonic poly (A+) RNA. The highest concentration of the two RNA species was observed in embryos which have terminated the process of somite formation.
D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000032:analysis; D005314:Embryonic and Fetal Development; D005399:Fishes / Q000196:embryology / Q000235:genetics*; D005801:Genes, Homeobox; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant; D012333:RNA, Messenger; D011061:Poly A