title: Xenobiotic metabolizing enzymes in spawning English sole (Parophrys vetulus) exposed to organic-solvent extracts of marine sediments from contaminated and reference areas.
abstract: Female English sole (Parophrys vetulus) within 1-2 days of spawning were exposed by i.m. injection to organic-solvent extracts of marine sediments at the following doses: Eagle Harbor (EHSE, contaminated site)--6.8 mg aromatic hydrocarbons (AHs)/kg body wt; Duwamish Waterway (DSE, contaminated site)--0.52 mg AHs and 0.040 mg chlorinated hydrocarbons (CHs)/kg body wt; Hood Canal (HCSE, reference site)--0.00090 mg AHs/kg body wt. Hepatic aryl hydrocarbon hydroxylase (AHH) activity, measured at spawning, was induced 10-, 23-and 2-fold by EHSE, DSE and HCSE, respectively, compared to sham and vehicle controls. Hepatic glutathione-S-transferase and epoxide hydrolase activities were not affected by any treatment. EHSE, but not DSE or HCSE, inhibited spawning (P less than 0.01) in 36% of the exposed fish and hepatic AHH activity in the non-spawning fish was significantly (P less than 0.05) higher than in the fish that did spawn. These results suggest a potential for reproductive toxicity in benthic fish after exposure to sediment-associated contaminants.
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis / Q000378:metabolism*; D004790:Enzyme Induction; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005260:Female; D005399:Fishes / Q000502:physiology*; D005982:Glutathione Transferase / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D012098:Reproduction; D012623:Seawater; D012997:Solvents; D014877:Water Pollution, Chemical
chemical_list: D006843:Hydrocarbons, Chlorinated; D011083:Polycyclic Compounds; D012997:Solvents; D001189:Aryl Hydrocarbon Hydroxylases; D005982:Glutathione Transferase; D004851:Epoxide Hydrolases