Effect of colchicine on some electrical properties of rat small intestine.
The effect of colchicine on the short circuit current (s.c.c.), potential difference (p.d.) and tissue resistance was investigated in vitro using rat jejunum. The electrogenic transfer of glucose, galactose, glycine and valine was also measured in the presence and absence of the drug. Colchicine (0.05 mM and 0.1 mM) caused a dose-dependent decrease in s.c.c. and p.d. in the presence of glucose but had no significant effect on the tissue resistance. Colchicine at (0.1 mM) increased the "apparent Km" of glucose (140% P less than 0.001) galactose (135% P less than 0.001) glycine (43% P less than 0.001) and valine (47% P less than 0.001) but had no significant effect on the p.d.max of these substrates.
D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D004553:Electric Conductivity; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D066298:In Vitro Techniques; D007583:Jejunum / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014633:Valine / Q000378:metabolism
D014633:Valine; D005947:Glucose; D003078:Colchicine; D005998:Glycine; D005690:Galactose