title: Atrial natriuretic peptide in bovine corpus luteum.
abstract: Atrial natriuretic peptide (ANP) has been demonstrated to exert endocrine functions, including the modulation of steroid synthesis. This prompted investigations to search for ANP receptors in the corpus luteum, a tissue that produces progesterone. The studies revealed a single binding site for [125I] ANP with similar characteristics (Kd, 122 pM; maximum binding, 18 fmol/mg protein) in all four stages of corpus luteum development. These receptors were demonstrated to stimulate cGMP production upon activation with synthetic ANP. Maximal cGMP synthesis was observed at 10(-7) M ANP, 5 min after activation of receptors. An acidic extract of corpus luteum contained immunoreactive ANP (approximately 220 fmol/g tissue), as indicated by gel chromatography, HPLC, and identification by means of a highly specific ANP antibody. The data do not permit definition of a specific endocrine role of ANP in the corpus luteum.
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003338:Corpus Luteum / Q000032:analysis / Q000378:metabolism*; D006152:Cyclic GMP / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006162:Guanylate Cyclase / Q000378:metabolism; D007700:Kinetics; D011863:Radioimmunoassay; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP