Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells.
Recent evidence shows that mature Thy-1 glycoprotein lacks amino acids 113-143 predicted from the cDNA sequence and is anchored to the plasma membrane by a phosphatidylinositol-containing glycolipid attached to amino acid 112. Previously characterized Thy-1-deficient mutant lymphoma lines of complementation classes A and E were analysed. They make detergent binding Thy-1 precursors but, in contrast to wild-type, the detergent binding moiety cannot be removed by phospholipase C. Moreover, tryptophan which only occurs at position 124 is incorporated into mutant but not parental Thy-1. This suggests that the mutants make a Thy-1 precursor of 143 amino acids but fail to replace its C-terminal end by a glycolipid anchor.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004247:DNA / Q000032:analysis; D005260:Female; D008223:Lymphoma / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009363:Neoplasm Proteins / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D010716:Phosphatidylinositols; D018800:Thy-1 Antigens; D004247:DNA