Molecular cloning and sequencing of cDNA encoding goat pre alpha-lactalbumin.
In poly(A)+RNA extracted from a lactating goat mammary gland, mRNA of about 750 nucleotides was shown to encode pre alpha-lactalbumin by using in vitro translation and immunoprecipitation. From the total poly(A)+RNA, the cDNA library was constructed using the Escherichia coli plasmid pUC18; it was screened with the oligodeoxyribonucleotide probe corresponding to the amino acid sequence of Trp60-Gln65 of goat alpha-lactalbumin. A plasmid containing almost full-length cDNA of goat pre alpha-lactalbumin, pGLA-1, was identified. The cDNA insert of pGLA-1 comprises 727 base pairs and contains the signal peptide and mature protein sequence.
D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005260:Female; D006041:Goats; D007120:Immunochemistry; D007768:Lactalbumin / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000032:analysis; D010957:Plasmids; D011061:Poly A / Q000302:isolation & purification; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000302:isolation & purification
D011498:Protein Precursors; D012333:RNA, Messenger; C034435:pre-alpha-lactalbumin; D011061:Poly A; D004247:DNA; D007768:Lactalbumin