Unusual alkaline elution pattern induced in mammalian cell DNA by fast neutrons p(34) + Be.
Syrian hamster fibroblasts (cell line BHK 21/13) were exposed to p(34) + Be fast neutron irradiation and their DNA analysed by the alkaline elution technique. The elution profiles showed an unusual tailing off, characteristic of neutron-irradiated samples, suggesting the presence of a modification in DNA induced by the neutrons. This was not seen with 60Co gamma-irradiation. In neutron-irradiated samples the alteration of DNA appeared to persist even after 2 h of post-treatment incubation (37 degrees C) indicating the absence of repair. The modification of DNA induced by neutrons provides a possible explanation for the reduction of the shoulders in survival curves obtained with neutrons, and the high RBE of neutrons.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D005214:Fast Neutrons; D066298:In Vitro Techniques; D008647:Mesocricetus; D009502:Neutrons
D004247:DNA