Detection of multidrug resistance markers, P-glycoprotein and mdr1 mRNA, in human leukemia cells.
We have examined the expression of P-glycoprotein in clinical leukemic cell samples by using a monoclonal antibody (MRK16) against P-glycoprotein. We found that leukemia cells isolated from 3 out of 6 patients with blast crisis of chronic myelogenous leukemia were reactive to MRK16. These 3 cell lines expressed high levels of mdr1 mRNA, which codes for P-glycoprotein. The present result indicates that the clinically refractory state of the tumor may be predicted in part by determining P-glycoprotein expression using the monoclonal antibody against P-glycoprotein, and the mdr1 probe.
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001752:Blast Crisis; D002460:Cell Line; D004351:Drug Resistance; D005784:Gene Amplification; D005796:Genes; D006801:Humans; D007951:Leukemia, Myeloid / Q000188:drug therapy / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins; D012333:RNA, Messenger