Analysis of antigens from membrane and soluble fractions of Entamoeba histolytica.
Axenic cultures of Entamoeba histolytica strains HK-9, HM-1, and Rahman were fractionated to provide plasma membranes, internal, vesiculated membranes, and a soluble cytosol. Each particulate fraction was solubilized and all fractions were analyzed by techniques designed to demonstrate molecular complexity and serologic reactivity. The cytosol contained more antigenic moieties than either membrane fraction; however, the antigens associated with the membranes had very high reactivity and lower nonspecific activity than the cytosol.
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D003600:Cytosol / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000276:immunology* / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay; D007158:Immunologic Techniques; D007425:Intracellular Membranes / Q000276:immunology
D000953:Antigens, Protozoan