title: Neuropeptide- and neurotransmitter-related immunoreactivities in the developing rat olfactory bulb.
abstract: The development of neuropeptide and neurotransmitter-related immunoreactivities in the rat olfactory bulb were investigated immunohistochemically by using antisera raised against substance P (SP), cholecystokinin-8 (CCK), neurotensin (NT), leucine-enkephalin or methionine-enkephalin-Arg6-Gly7-Leu8 (ENK), somatostatin (SOM), neuropeptide Y (NPY) and tyrosine hydroxylase (TH). Results obtained for the adult olfactory bulb confirmed previous observations, except for SP-like immunoreactive (SP-IR) granule cells in the main olfactory bulb (MOB) and NT-IR neurons around the modified glomerular complex (MGC) (Teicher et al., Brain Res. 194:530-535, 1980). SP-, CCK- and NT-IR neurons were observed in the MOB of the rat fetus. SP-IR neurons also appeared in the accessory olfactory bulb (AOB). Among them, NT-IR neurons in the MOB and SP-IR neurons in the AOB were observed on embryonic day 16. SP- and CCK-IR neurons in the MOB appeared on embryonic day 18. Most of these neurons were presumed to be projecting neurons. SOM-, NPY-, ENK- and TH-IR neurons appeared in the newborn rats. The number and intensity of immunostaining of these neurons continued to increase with age, producing the adult pattern, except for NT-IR neurons in the MGC and SP-IR neurons in the mitral cell layer of the AOB, which were more numerous and intensely stained in young animals.
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development* / Q000276:immunology; D005314:Embryonic and Fetal Development; D007150:Immunohistochemistry; D009479:Neuropeptides / Q000276:immunology*; D018377:Neurotransmitter Agents / Q000276:immunology*; D009830:Olfactory Bulb / Q000196:embryology / Q000276:immunology*; D051381:Rats / Q000196:embryology / Q000254:growth & development / Q000276:immunology*; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D009479:Neuropeptides; D018377:Neurotransmitter Agents