A surface component on GH3 pituitary cells that recognizes transforming growth factor-beta, activin, and inhibin.
We have examined the ability of various forms of activin and inhibin, which are structurally related to transforming growth factor-beta (TGF-beta), to interact with various types of cell surface TGF-beta binding sites. Activin AB, inhibin A, and inhibin B were unable to compete with 125I-TGF-beta 1 for binding to the TGF-beta receptor types I, II, or III that coexist in human skin fibroblasts, rat liver epithelial cells, and mink lung epithelial cells. In contrast, activins and inhibins effectively competed for TGF-beta 1 binding to GH3 rat pituitary tumor cells. Binding of TGF-beta 1 to GH3 cells was mediated by about 2700 sites/cell with a Kd = 90 pM. Affinity labeling of these GH3 binding sites by cross-linking to 125I-TGF-beta 1 yielded 70-74-kDa labeled complexes distinct from previously identified TGF-beta binding components. Labeling of these 70-74-kDa components with 125I-TGF-beta 1 was inhibited by TGF-beta 1, TGF-beta 2, activin AB, and inhibin B at concentrations in the high picomolar to low nanomolar range, but it was not significantly affected by other polypeptide hormones and growth factors tested. The 70-74-kDa labeled GH3 components represent a novel type of cell surface TGF-beta binding protein that is unique in its ability to recognize various other members of the TGF-beta family of bioactive polypeptides.
D029404:Activin Receptors; D028341:Activins; D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D007265:Inhibins / Q000378:metabolism*; D007700:Kinetics; D009842:Oligopeptides; D010455:Peptides / Q000378:metabolism*; D010911:Pituitary Neoplasms; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; D015291:Transforming Growth Factors / Q000378:metabolism*
D009842:Oligopeptides; D010455:Peptides; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; C044147:activin B; D028341:Activins; C048617:myelopeptides; D007265:Inhibins; D015291:Transforming Growth Factors; D029404:Activin Receptors