Chemoresponsiveness of intracellular nuclei neurones to L-aspartate, L-glutamate and related derivatives in rat cerebellar slices maintained in vitro.
The sensitivity of intracerebellar nuclei neurones to pulse applications of L-aspartate, L-glutamate, N-methyl-D,L-aspartate and quisqualate was tested in rat cerebellar slices maintained in vitro. The responses of the nuclear neurones to the four agonists consisted of a transient and dose-dependent increase in their firing of simple spikes. When suprathreshold currents were used, quisqualate induced the highest increase in the spike discharge frequency of the cells. Quisqualate mediated responses were unaffected by steady applications of 2-amino-5-phosphonovalerate, whereas the sensitivity of the responses induced by the three other agonists was in the order N-methyl-D,L-aspartate, L-aspartate, L-glutamate. When the superfusing solution was devoid of Mg2+ ions, N-methyl-D,L-aspartate and L-aspartate mediated responses were much potentiated, while quisqualate induced responses were not enhanced. In such a medium, L-glutamate elicited responses were more or less potentiated depending on cells. These results suggest that rat intracerebellar nuclei neurones bear both N-methyl-D-aspartate and non-N-methyl-D-aspartate, probably quisqualate, receptors, and that L-aspartate and L-glutamate have a mixed action upon both types. L-Aspartate preferentially activates N-methyl-D-aspartate receptors, whereas L-glutamate predominantly acts via non-N-methyl-D-aspartate receptors. Furthermore, the potency of L-glutamate in activating N-methyl-D-aspartate receptors appears to vary as a function of the cells.
D000818:Animals; D001224:Aspartic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D002529:Cerebellar Nuclei / Q000187:drug effects / Q000502:physiology*; D003292:Convulsants / Q000494:pharmacology; D005971:Glutamates / Q000008:administration & dosage / Q000494:pharmacology*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D007267:Injections; D008274:Magnesium / Q000494:pharmacology; D016202:N-Methylaspartate; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010069:Oxadiazoles / Q000494:pharmacology; D016318:Quisqualic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship
D003292:Convulsants; D005971:Glutamates; D010069:Oxadiazoles; D001224:Aspartic Acid; D018698:Glutamic Acid; D016202:N-Methylaspartate; D016318:Quisqualic Acid; D008274:Magnesium