Safe, live Vibrio cholerae vaccines?
Mutants of Vibrio cholerae defective in intestinal colonization have been constructed. Characterization of these mutants has led to the identification of a gene cluster involved in the assembly of a pilus colonization factor called TCP. The tcp operon has been cloned and strains of V. cholerae have been constructed that overproduce this pilus and the B subunit of cholera toxin. Together these studies may contribute to the eventual construction of efficient live and killed, oral cholera vaccines.
D000284:Administration, Oral; D022121:Cholera Vaccines / Q000276:immunology / Q000302:isolation & purification*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D010861:Fimbriae, Bacterial / Q000276:immunology*; D005786:Gene Expression Regulation; D009154:Mutation; D014613:Vaccines, Attenuated / Q000276:immunology / Q000302:isolation & purification; D014734:Vibrio cholerae / Q000235:genetics*
D022121:Cholera Vaccines; D004251:DNA Transposable Elements; D014613:Vaccines, Attenuated