Distribution of contractile proteins in single isolated smooth muscle cells from the ascidian body-wall muscle.
1. Relaxed cells isolated from ascidian body-wall muscle were morphologically very similar to relaxed common smooth muscle cells. 2. The contracted cells, however, possessed striations which were resolved into a repeating pattern of light and dark bands using phase contrast microscope. 3. The relaxed ascidian cells treated with Triton X-100 were contracted and showed the striations by adding Ca2+. 4. By an indirect immunofluorescence method, it was clearly seen that antiactin spread uniformly in the relaxed cells, while this antibody was concentrated on the dark bands of striations in the contracted cells.
D000199:Actins / Q000032:analysis; D000818:Animals; D003285:Contractile Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D009130:Muscle, Smooth / Q000032:analysis*; D012016:Reference Values; D014561:Urochordata
D000199:Actins; D003285:Contractile Proteins