Glutamate-elicited stimulation of acetylcholinesterase activity in cerebellar slices from newborn rats.
Bath application of glutamate at two concentration ranges, 10(-6)-10(-8) and 1-3 X 10(-3) M, effectively increased acetylcholinesterase activity in cerebellar slices obtained from 8-day-old rats. No such effect was seen in cerebellar slices of 7-week-old rats or cerebral slices of either 7-week or 8-day-old rats. Glutamic acid diethyl ester blocked the glutamate effect at both of these concentration ranges, suggesting that quisqualate-sensitive glutamate receptors are involved in regulation of acetylcholinesterase activity in early postnatal cerebellum. Since bath application of cyclic GMP at 10(-7)-10(-9) M increased the acetylcholinesterase activity in cerebellar slices of 8-day-old rats, it is possible that glutamate-dependent regulation of acetylcholinesterase activity is mediated by cyclic GMP. The observation that adenosine deaminase blocked the effect of glutamate completely at 10(-6)-10(-8) M and partially at 1-5 X 10(-3) M further suggests that release of adenosine is a link from enhanced cyclic GMP activity to activation of acetylcholinesterase.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002531:Cerebellum / Q000201:enzymology* / Q000254:growth & development; D002540:Cerebral Cortex / Q000201:enzymology* / Q000254:growth & development; D006152:Cyclic GMP / Q000494:pharmacology; D005260:Female; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D051381:Rats; D011919:Rats, Inbred Strains
D005971:Glutamates; D018698:Glutamic Acid; D000110:Acetylcholinesterase; D006152:Cyclic GMP