Stimulation of superoxide anion formation by the non-TPA type tumor promoters palytoxin and thapsigargin in porcine and human neutrophils.
The non-12-O-tetradecanoylphorbol-13-acetate (TPA) type tumor promoters palytoxin and thapsigargin provoked a respiratory burst in porcine and human neutrophils. The amounts of oxygen consumed and superoxide anion (O2-) produced were found to be stoichiometric. Concentrations of 6.5 X 10(-8) M palytoxin and 1.2 X 10(-6) M thapsigargin were required for half-maximal stimulation to 3 X 10(6) porcine cells/ml in Hanks' solution. Combinations of palytoxin and thapsigargin, and of one non-TPA type and one TPA-type tumor promoter, had synergistic effects in stimulating O2- formation in neutrophils, suggesting that these compounds activate the cells by different signal transduction mechanisms.
D000178:Acrylamides; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D003064:Cnidarian Venoms / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008235:Lyngbya Toxins / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D019255:NADPH Oxidases; D010101:Oxygen Consumption / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D019284:Thapsigargin; D000095702:Polyether Toxins
D000178:Acrylamides; D002273:Carcinogens; D003064:Cnidarian Venoms; D008235:Lyngbya Toxins; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D010936:Plant Extracts; D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate; D019284:Thapsigargin; C022176:teleocidins; C010272:palytoxin; D000095702:Polyether Toxins