IL-2 production in human T lymphotropic virus I-infected leukemic T lymphocytes analyzed by in situ hybridization.
In situ hybridization studies were performed with 35S-labeled anti-sense RNA probes to study IL-2 mRNA expression in three human T lymphotropic virus I-infected T cell lines at the single cell level. In HuT 102, MT-2, and MT-4 cells, IL-2 mRNA-expressing cells were identified, occurring at frequencies of 2 x 10(-2), 8 x 10(-3), and 5 x 10(-3), respectively. In these cell lines, IL-2 mRNA was not detectable in RNA extracted from whole adult T cell leukemia cell populations because of dilution by other RNA species from the vast majority of cells that do not contain IL-2 mRNA. The data indicate the possibility of paracrine growth stimulation via IL-2 and its receptor even in those human T lymphotropic virus I-infected T cell populations that apparently lack IL-2 activity when analyzed by conventional assay procedures.
D002460:Cell Line; D017977:Deltaretrovirus; D006800:Deltaretrovirus Infections / Q000235:genetics / Q000378:metabolism*; D006680:HLA Antigens / Q000302:isolation & purification; D015793:HLA-B7 Antigen; D006801:Humans; D007118:Immunoassay; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000302:isolation & purification
D006680:HLA Antigens; D015793:HLA-B7 Antigen; D007376:Interleukin-2; D012333:RNA, Messenger