Detection of Clostridium perfringens epsilon toxin by ELISA.
An enzyme-linked immunosorbent assay (ELISA) has been developed as an alternative to neutralisation tests in mice to detect Clostridium perfringens type D epsilon toxin in the intestinal contents of animals which have died from suspected enterotoxaemia. The test was sensitive and quantitative and gave excellent agreement with the mouse protection test.
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D003015:Clostridium Infections / Q000662:veterinary*; D003016:Clostridium perfringens; D004767:Enterotoxemia / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D051379:Mice; D009500:Neutralization Tests; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D001427:Bacterial Toxins; C039811:Clostridium perfringens epsilon-toxin