title: ATP synthase complex from beef heart mitochondria. Separation of protein bands in the region of 28-31 kDa.
abstract: ATPase/ATP synthase preparations originally contain protein bands in the 28-31-kDa region. The present study demonstrates separation of the band at 29 kDa (adenine nucleotide translocator) from a band at approximately 31 kDa. In cholate/ammonium sulfate-extracted ATP synthases removal of the 31-kDa band results in decrease of ATP-Pi exchange and oligomycin sensitivity of the ATPase activity. It is suggested that the protein band at 31 kDa is heterogeneous, containing diverse activities, the identities of which are yet to be determined.
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D002417:Cattle; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D008929:Mitochondria, Heart / Q000201:enzymology*; D000226:Mitochondrial ADP, ATP Translocases / Q000032:analysis; D008970:Molecular Weight; D009840:Oligomycins / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000302:isolation & purification*
chemical_list: D009840:Oligomycins; D000226:Mitochondrial ADP, ATP Translocases; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases