Isolation of mutants of Schizosaccharomyces pombe unable to synthesize cadystin, small cadmium-binding peptides.
Schizosaccharomyces pombe synthesize small cadmium-binding peptides cadystin, structure of which is (gamma-Glu-Cys)n-Gly, in response to cadmium. Mutants unable to synthesize cadystin were found in the mutants hypersensitive to cadmium. Some of them lack activity of either gamma-glutamylcysteine synthetase (EC 6.3.2.2) or glutathione synthetase (EC 6.3.2.3), enzyme involved in glutathione biosynthesis. Some mutants have the same activity levels of these enzymes as wild type has. These results indicate that some steps of cadystin biosynthesis are catalyzed by the enzymes catalyzing glutathione biosynthesis.
D002104:Cadmium / Q000378:metabolism* / Q000494:pharmacology; D002352:Carrier Proteins / Q000096:biosynthesis*; D002850:Chromatography, Gel; D005656:Fungal Proteins / Q000096:biosynthesis*; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis; D005981:Glutathione Synthase / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008668:Metallothionein / Q000096:biosynthesis*; D009154:Mutation; D004718:Saccharomycetales / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins
D002352:Carrier Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D029702:Schizosaccharomyces pombe Proteins; C004203:cadmium-binding protein; D002104:Cadmium; C055088:cadystin protein, S pombe; D008668:Metallothionein; D005721:Glutamate-Cysteine Ligase; D005981:Glutathione Synthase; D005978:Glutathione