title: Cholinergic modulation of the release of serotonin in the gastric interstitial fluid. An in vivo study in rabbits.
abstract: Dialysis fibers chronically implanted into the gastric submucosa of rabbits allowed us to simultaneously (a) collect an interstitial fluid dialysate in which 5-hydroxytryptamine concentrations were measured, and (b) locally perfuse drugs such as acetylcholine, neostigmine, and atropine, which stimulated the release of 5-hydroxytryptamine. The effect of acetylcholine was not blocked by atropine but was blocked by hexamethonium. Furthermore, 5-hydroxytryptamine concentrations in the interstitial fluid were lower when acetylcholine and hexamethonium were injected together than when hexamethonium was injected alone. We conclude that acetylcholine stimulates the release of 5-hydroxytryptamine into the gastric interstitial fluid by acting on nicotinic receptors, and has inhibitory effects by stimulating the muscarinic receptors.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002799:Cholinergic Fibers / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D009388:Neostigmine / Q000494:pharmacology; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism*
chemical_list: D006584:Hexamethonium Compounds; D012701:Serotonin; D009388:Neostigmine; D018738:Hexamethonium; D001285:Atropine; D000109:Acetylcholine