Release of i-r metenkephalin from rat amygdala slices in vitro.
Release of immuno-reactive metenkephalin has been demonstrated from slices of rat amygdala in vitro. This release was calcium dependent and the amount released was related to the concentration of potassium ions in the releasing stimulus. The effect of drugs such as clonidine, an alpha 2 adrenoceptor agonist, and idazoxan, an antagonist, on this release was measured. Neither drug had a significant effect on the amount of ir-metenkephalin released. Thus although release of noradrenaline can be affected by opioids it seems that release of endogenous opioids is not affected by drugs which interact with noradrenaline.
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000679:Amygdala / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003000:Clonidine / Q000494:pharmacology; D004146:Dioxanes / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000378:metabolism*; D019329:Idazoxan; D066298:In Vitro Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D000319:Adrenergic beta-Antagonists; D004146:Dioxanes; D004744:Enkephalin, Methionine; D003000:Clonidine; D011188:Potassium; D002118:Calcium; D019329:Idazoxan