Analysis of amino acids: neurochemical application.
For high sensitivity analysis of neuroactive amino acids, liquid chromatography employing precolumn derivatisation with o-phthalaldehyde (OPA) is suitable for several reasons. The OPA reagent is non-fluorescent per se, the reaction occurs rapidly in alkaline aqueous solutions and forms highly fluorescent derivatives with primary amines.
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents; D008832:Microchemistry; D018377:Neurotransmitter Agents / Q000032:analysis*; D009764:o-Phthalaldehyde
D000596:Amino Acids; D007202:Indicators and Reagents; D018377:Neurotransmitter Agents; D009764:o-Phthalaldehyde