title: Formation of rat copulatory plug: purified seminal vesicle secretory proteins serve as transglutaminase substrates.
abstract: An in vitro system has been used to study the role of purified rat seminal vesicle proteins in the formation of the copulatory vaginal plug. Proteins II, IV (or S) and V (or F) were each separately coagulated using the transglutaminase in coagulating gland extracts. In each case the coagulum required Ca2+ ions for its formation and was insoluble in denaturing solvents. In experiments with [3H]lysine, proteins II and S incorporated [3H]lysine into glu-lys dipeptide with similar kinetics. Both the N-terminal and C-terminal glutamine residues of protein S participated in the reaction.
mesh_terms: D000818:Animals; D003307:Copulation; D005260:Female; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology*; D051381:Rats; D012669:Seminal Vesicles / Q000378:metabolism*; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D011506:Proteins; D011503:Transglutaminases; D008239:Lysine