title: Localization of pancreastatin immunoreactivity in porcine endocrine cells.
abstract: Pancreastatin is a peptide isolated from the porcine pancreas and shown to inhibit insulin release. We have studied the immunocytochemical distribution of pancreastatin in three porcine endocrine tissues: pancreas, gut, and adenohypophysis. Pancreastatin-specific immunoreactivity was found in all three locations and distributed to numerous cells. In the pancreas, we performed the alternate labeling of consecutive thick (immunofluorescence) or thin (protein A-gold) sections and we observed that pancreastatin colocalizes to secretory granules of insulin and somatostatin-containing cells. The relationship of pancreastatin to chromogranin A is discussed.
mesh_terms: D001078:APUD Cells / Q000032:analysis*; D000818:Animals; D053379:Chromogranin A; D004064:Digestive System / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D010179:Pancreas / Q000032:analysis*; D010187:Pancreatic Hormones / Q000032:analysis* / Q000276:immunology; D010902:Pituitary Gland / Q000032:analysis; D013552:Swine
chemical_list: D053379:Chromogranin A; D007106:Immune Sera; D010187:Pancreatic Hormones; C050836:pancreastatin