An HPLC assay for sulphapyridine in plasma and its use to assess small bowel transit time after the administration of sulphasalazine.
An HPLC assay for sulphapyridine is described. The use of the assay to measure sulphapyridine produced by the action of large bowel flora on 2 g of orally administered sulphasalazine as a means of assessing oro-colonic transit time is discussed. The assay is applied to show the action on oro-colonic transit time of a novel gastrokinetic agent BRL 24924 which brought about a 62.8% median reduction in transit time at a dose of 5 mg.
D000328:Adult; D001549:Benzamides / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002851:Chromatography, High Pressure Liquid; D005769:Gastrointestinal Motility / Q000187:drug effects*; D006801:Humans; D008297:Male; D012460:Sulfasalazine / Q000097:blood*
D001549:Benzamides; D019086:Bridged Bicyclo Compounds, Heterocyclic; D012460:Sulfasalazine; C053006:renzapride