Corticotropin-like material in pigeon brains.
1. Pigeon brain acetone powder was extracted with a mixture of acetone, water and hydrochloric acid (40:21:1 v/v/v). The extract was added to a large volume of chilled acetone. 2. The resultant precipitate was washed with cold acetone and then chromatographed on Sephadex G-25. The void volume peak constituted fraction A. The trailing peak immediately following fraction A was divided into two fractions designated B and C. 3. Fraction B (mol. wt less than 5,000) stimulated corticosterone production in isolated rat adrenal cells while the other fractions were devoid of similar ACTH-like bioactivity. 4. None of the fractions showed activity in the opiate radioreceptor assay or hot plate test, implying the absence or presence of only trace amounts of opiate-like materials. Other possibilities that cannot be ruled out include the presence of molecules with substitutions in the sequence by amino acids that result in little or no activity.
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000818:Animals; D001681:Biological Assay; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D010856:Columbidae / Q000378:metabolism*; D008297:Male; D008566:Membranes / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000378:metabolism
D011957:Receptors, Opioid; D000324:Adrenocorticotropic Hormone