Detection of sequences that hybridize to human cytomegalovirus DNA in cervical neoplastic tissue.
Punch biopsies were taken from patients with cervical intraepithelial neoplasia and the DNA was extracted and examined for sequences which hybridized to human cytomegalovirus DNA by Southern blotting analysis. Two biopsies out of 43 were found to contain DNA which hybridized to human cytomegalovirus DNA. In one biopsy DNA sequences were detected which contained four restriction sites colinear with those of the prototype strain AD169. Evidence of rearranged viral DNA sequences was also found.
D002278:Carcinoma in Situ / Q000032:analysis* / Q000382:microbiology; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D002583:Uterine Cervical Neoplasms / Q000032:analysis* / Q000382:microbiology
D004273:DNA, Neoplasm; D004279:DNA, Viral