A unique T-cell line derived from an HTLV-1-negative adult T-cell leukemia patient.
A unique T-cell line, designated ATL-5T, was established from lymphoma cells in pericardial effusion of an adult T-cell leukemia (ATL) patient not carrying HTLV-1 provirus. The cell line is OKT4 and/or Leu3a+ and OKT8 and/or Leu2a+, but interleukin 2 receptor (IL2R)- and HTLV-1 provirus genome negative, and has cytogenetically abnormal karyotypes. The cell line contains rearranged T-cell receptor beta-chain gene, which was identical in rearrangement pattern to the T-cell receptor beta-chain gene in primary cells. These results suggest that factors other than HTLV-1 may sometimes be associated with HTLV-1-negative ATL. The ATL-5T cell line we describe here is unique, and should contribute to further elucidation of the mechanisms involved in the pathogenesis of HTLV-1-negative ATL and HTLV-1-positive ATL.
D004279:DNA, Viral / Q000032:analysis; D017977:Deltaretrovirus / Q000032:analysis* / Q000235:genetics; D006800:Deltaretrovirus Infections / Q000382:microbiology / Q000473:pathology*; D005796:Genes; D005814:Genes, Viral; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D013601:T-Lymphocytes / Q000382:microbiology / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000382:microbiology
D004279:DNA, Viral; D011948:Receptors, Antigen, T-Cell