The discrimination between human, porcine and bovine insulin with 1H NMR spectroscopy.
The 1H- and 1H-1H correlation (COSY) NMR spectra of human, porcine and bovine insulin have been recorded. The 1.1-1.5 ppm regions of these spectra show the methyl signals of the alanine and threonine residues. The number of alanines and threonines differs for the three insulins and thus allows an easy discrimination with NMR.
D000818:Animals; D002417:Cattle; D006801:Humans; D007328:Insulin / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013045:Species Specificity; D013552:Swine
D007328:Insulin