title: Sequestration of environmental cadmium by metallothionein in the roach (Rutilus rutilus) and the stone loach (Noemacheilus barbatulus).
abstract: Two species of coarse fish that are relatively resistant to cadmium poisoning were exposed to sub-lethal concentrations of the metal in their aquarium water. Thus, roach were exposed to cadmium concentrations between 30 and 500 micrograms/l for periods of 14-70 days whereas stone loach were exposed to 1250 micrograms Cd/l for 21-77 days. Under all conditions of exposure, it was found upon analysis of the major organs of accumulation of cadmium in the two species that the toxic metal was sequestered by a single isoform of metallothionein. The amino acid compositions of roach and stone loach metallothionein were determined and found to be similar to those reported for other piscine metallothioneins. The two proteins were found to contain Cd:Zn:Cu in approximate ratios of 4:1:2 per mole of protein. The sequestration of Cd by metallothionein in the two resistant species of fish is contrasted with the situation observed previously in a cadmium-sensitive species, the rainbow trout.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002104:Cadmium / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D003530:Cyprinidae / Q000378:metabolism*; D003531:Cypriniformes / Q000378:metabolism*; D005412:Flatfishes / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008668:Metallothionein / Q000302:isolation & purification / Q000378:metabolism*; D014337:Trout / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002104:Cadmium; D003300:Copper; D008668:Metallothionein; D015032:Zinc