Comparison of basal glutathione S-transferase activities and of the influence of phenobarbital, butylated hydroxy-anisole or 5,5'-diphenylhydantoin on enzyme activity in male rodents.
1. Hepatic cytosolic glutathione S-transferase (GST) activities, toward five substrates, were shown to vary markedly among three laboratory rodent species. 2. Basal GST activities for 1-chloro-2,4-dinitrobenzene (hamster greater than mouse greater than rat), 1,2-dichloro-4-nitrobenzene (mouse greater than rat greater than hamster), p-nitrobenzyl chloride (rat = mouse = hamster), bromosulfophthalein (rat greater than mouse greater than hamster) and 1,2-epoxy-3-(p-nitrophenoxy)propane (mouse greater than rat = hamster) differed with respect to magnitude and distribution among species. 3. GST substrate activities in response to phenobarbital, butylated hydroxy-anisole or 5,5'-diphenylhydantoin treatment were increased more often in mouse and rat as compared to the hamster. 4. These results suggest that basal GST activity, as well as inducibility, differ among rodent species. Since GST are involved in detoxication processes, differences in GST properties may underlie variability in species sensitivity to toxicants.
D000818:Animals; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D006224:Cricetinae / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008647:Mesocricetus / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D010672:Phenytoin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains / Q000378:metabolism*; D013045:Species Specificity
D002083:Butylated Hydroxyanisole; D010672:Phenytoin; D005982:Glutathione Transferase; D010634:Phenobarbital