title: Biochemical studies on acetate non-utilizing mutants of Aspergillus terreus IRRL 16043.
abstract: The strain Aspergillus terreus IRRL 16043 can utilize glucose as well as acetate as a sole carbon source. Thirty-nine mutants were isolated from the wild-type by treatment with a chemical mutagen, N-methyl-N'-nitro-N-nitrosoguanidine (MNTG) which could not utilize acetate as a sole carbon source, and were designated as acetate non-utilizing (acu). By complementation and biochemical analyses they were divided into three functional groups, acu A, acu B and acu C lacking isocitrate lyase, malate synthase and acetyl-CoA synthetase activity, respectively.
mesh_terms: D000106:Acetate-CoA Ligase / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D001230:Aspergillus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005816:Genetic Complementation Test; D007522:Isocitrate Lyase / Q000378:metabolism; D008292:Malate Synthase / Q000378:metabolism; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation
chemical_list: D000085:Acetates; D008769:Methylnitronitrosoguanidine; D008292:Malate Synthase; D007522:Isocitrate Lyase; D000106:Acetate-CoA Ligase