Tenebrosin-A, a new cardiostimulant protein from the Australian sea anemone Actinia tenebrosa.
A new cardiac stimulatory protein, tenebrosin-A, has been isolated from the Australian sea anemone Actinia tenebrosa by gel filtration and cation-exchange chromatography, followed by cation-exchange HPLC. Its purity is established by analytical reversed-phase HPLC and N-terminal sequence analysis. According to SDS-PAGE, its apparent Mr is 20,000 daltons. Amino acid analysis indicates that it contains 186 residues, and is devoid of cysteine or cystine. Tenebrosin-A exerts a strong positive inotropic effect on isolated guinea pig atria at a concentration of 1.4 nM, with little chronotropic activity.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003063:Cnidaria / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007447:Invertebrate Hormones / Q000494:pharmacology*; D009200:Myocardial Contraction / Q000187:drug effects; D012615:Sea Anemones / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D002316:Cardiotonic Agents; D007447:Invertebrate Hormones; C054800:tenebrosin A