Tetanic stimulation-induced changes in [3H]glutamate binding and uptake in rat hippocampus.
Tetanus-induced (400 Hz, 200 pulses) long-lasting potentiation of the stratum radiatum-evoked CA1 population spike in hippocampal slices is not accompanied by any change in Na+-independent [3H]glutamate binding sites. Homosynaptic depression that occurs subsequent to either a low frequency tetanus (20 Hz, 600 pulses) or a transient exposure to Cl(-)-free (containing NO3-) medium is associated with an elevation in the amino acid binding. [3H]Glutamate uptake into slices was decreased following a high frequency (400 Hz, 200 pulses) tetanus but in the majority of cases was increased following a low frequency (20 Hz, 600 pulses) tetanus to stratum radiatum. When the high frequency tetanus was given in the absence of extracellular Ca2+, there was a further reduction in [3H]glutamate uptake.
D000818:Animals; D001665:Binding Sites; D001693:Biological Transport, Active; D004558:Electric Stimulation; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000378:metabolism
D005971:Glutamates; D018698:Glutamic Acid