title: Development of hypothalamic neurons in intraventricular grafts: expression of specific transmitter phenotypes.
abstract: The anlages of the medial-basal hypothalamus (MBH), septopreoptic area (POA), Rathke's pouch, and the parietal cortex (CC) of rats (at 12.5, 14.5 and 16.5 days of gestation) were transplanted singly or in combination into the third ventricle of adult female rats, and the development of neurons in the grafts was investigated immunohistochemically with the use of antisera to tyrosine hydroxylase (TH), somatostatin (SRIH), ACTH, methionine enkephalin-Arg6-Gly7-Leu8 (Enk-8), rat corticotropin-releasing factor (rCRF), rat hypothalamic growth hormone-releasing factor (rhGRF), and luteinizing hormone-releasing hormone (LHRH). TH and all the peptides examined except LHRH were detected in distinct neurons in MBH grafts and in cografts of MBH plus Rathke's pouch from 12.5-day-old embryos. SRIH, rCRF, Enk-8, and TH were found in POA grafts from embryos of the same age. Although immunoreactive LHRH was first detected in neurons in POA grafts from 16.5-day-old embryos, it appeared in cografts of POA and MBH from 12.5-day-old embryos. The immunoreactive fibers developed in the grafts expressed the same characteristic behaviors as in intact brain; the fibers containing hormonal substances formed complexes with the vasculature like in the organum vasculosum laminae terminalis (OVLT) or in the median eminence, while the fibers containing neurotropic signals formed fiber networks surrounding other nerve cell bodies as if they synaptically associate. In CC grafts, the neurons contained TH, SRIH, rCRF, or Enk-8, and their axonal processes formed fiber networks. These findings suggest that all the hypothalamic neurons examined are committed by 12.5 days of gestation to develop maintaining transmitter phenotype and target recognition capacity.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D002454:Cell Differentiation; D002552:Cerebral Ventricles / Q000254:growth & development; D004744:Enkephalin, Methionine / Q000032:analysis; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis; D007033:Hypothalamus, Middle / Q000254:growth & development* / Q000637:transplantation; D007150:Immunohistochemistry; D009474:Neurons / Q000032:analysis / Q000254:growth & development*; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D007987:Gonadotropin-Releasing Hormone; D013004:Somatostatin; D004744:Enkephalin, Methionine; D000324:Adrenocorticotropic Hormone; D014446:Tyrosine 3-Monooxygenase