Immunoreactive dynorphin-A in ovine anterior pituitary and effects of estradiol-17 beta administration.
Anterior lobe (AL) tissue of the ovine pituitary gland contained a form of immunoreactive dynorphin-A (ir-DYN-A) larger than that found in pituitary neurointermediate lobe. Administration of estradiol-17 beta or estradiol-17 beta plus progesterone to ovariectomized sheep decreased AL tissue concentrations of ir-DYN-A but did not affect any LH parameter. Enzymatically dispersed AL cells also contained ir-DYN-A, but specific release during in vitro incubation was too low to be detected even when cells were exposed to gonadotropin-releasing hormone.
D000818:Animals; D002478:Cells, Cultured; D004399:Dynorphins / Q000032:analysis / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D010052:Ovariectomy; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods; D012756:Sheep
D004958:Estradiol; D004399:Dynorphins