An electrophoretic method for the determination of the proportion of gamma-aminobutyric acid in a mixture of labeled neurotransmitter and its catabolites.
An electrophoretic method for the separation of gamma-aminobutyric acid (GABA) from its metabolites after GABA-transaminase attack is presented. The method is based on the fact that at neutral pH GABA has no net electrical charge, whereas its major metabolites, succinic acid and Krebs cycle intermediates, are negatively charged. The method appears to be especially suitable for evaluation of true-labeled neurotransmitter within the radioactivity which is found in synaptosomes after labeled GABA-uptake studies.
D000612:4-Aminobutyrate Transaminase; D000818:Animals; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000379:methods; D018377:Neurotransmitter Agents / Q000032:analysis*; D011865:Radioisotope Dilution Technique; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000378:metabolism
D002250:Carbon Radioisotopes; D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid; D000612:4-Aminobutyrate Transaminase