Localization of the ornithine aminotransferase gene and related sequences on two human chromosomes.
We have used a full length cDNA clone to determine the chromosomal location of the gene encoding human ornithine aminotransferase (OAT), a mitochondrial matrix enzyme. Southern blot analysis of Sca I-digested DNA from 34 human-mouse somatic cell hybrids revealed 11 human fragments. Three fragments mapped to chromosome 10q23-10qter, confirming the previous provisional assignment of the functional gene to this autosome by analysis of OAT expression in somatic cell hybrids (O'Donnell et al. 1985). The remaining eight fragments were assigned to the X chromosome, and regionally assigned to Xp21-Xp11 by use of an X-chromosome mapping panel. These X chromosome sequences could represent pseudogenes, or related members of a multigene family. Two of the X chromosome fragments are alternate alleles of a restriction fragment length polymorphism (RFLP) making this OAT-related locus an excellent genetic marker. The RFLP may now be used to determine any possible relationship between this locus and several X-linked eye defects.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D000637:Transaminases / Q000235:genetics*; D014960:X Chromosome
D005819:Genetic Markers; D004247:DNA; D000637:Transaminases; D009953:Ornithine-Oxo-Acid Transaminase; D004262:DNA Restriction Enzymes; C047020:endodeoxyribonuclease ScaI; D015252:Deoxyribonucleases, Type II Site-Specific