Somatic cell mapping and restriction fragment analysis of bovine genes for fibronectin and gamma crystallin.
DNAs from cow-hamster and cow-mouse somatic hybrid cells segregating bovine chromosomes have been analyzed by Southern blotting and hybridization with human fibronectin and gamma crystallin probes. Concordancy of retention of these bovine genes was compared to cattle isozyme loci representing previously described syntenic groups. Bovine fibronectin (FNI) and gamma crystallin (CRYG) fragments were concordant with each other and with isocitrate dehydrogenase 1 (IDH1), representing the bovine syntenic group U17. The syntenic relationship of these genes is conserved on human chromosome 2q and also on mouse chromosome 1. In addition, bovine RFLPs were identified with both fibronectin and gamma crystallin probes. These polymorphisms will be used to study recombination between the syntenic loci in pedigreed herds and to mark a segment of the bovine genome that is likely homologous to the Lsh region of mouse chromosome 1, which confers resistance in mice to several intracellular parasites.
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D003459:Crystallins / Q000235:genetics*; D005353:Fibronectins / Q000235:genetics*; D005819:Genetic Markers; D006822:Hybrid Cells; D051379:Mice; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D003459:Crystallins; D005353:Fibronectins; D005819:Genetic Markers