In-vitro biotransformation of antipyrine, lignocaine and propranolol in the liver of rats with turpentine-induced inflammation.
In rats with inflammation induced by turpentine injection, changes in drug disposition occur in-vivo and in the perfused isolated liver. Therefore the biotransformation of a low extraction drug, antipyrine, and of two high extraction drugs, lignocaine and propranolol, has been evaluated in the 9000g supernatant fraction of the liver of turpentine-treated rats. Aminopyrine N-demethylase activity and cytochrome P450 content were also measured. Turpentine treatment significantly reduced the in-vitro breakdown of the three drugs; aminopyrine N-demethylase activity and cytochrome P450 content were also decreased. Similar results were found in the proadifen-treated rats, except that in those, the cytochrome P450 content was slightly increased. The changes in drug disposition seen after turpentine-induced inflammation, could therefore be due in part to a change in hepatic enzymatic activity.
D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D000983:Antipyrine / Q000493:pharmacokinetics*; D001711:Biotransformation; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006520:Hepatitis, Animal / Q000378:metabolism; D066298:In Vitro Techniques; D008012:Lidocaine / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011433:Propranolol / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains; D014425:Turpentine
D003577:Cytochrome P-450 Enzyme System; D008012:Lidocaine; D011433:Propranolol; D000633:Aminopyrine N-Demethylase; D000983:Antipyrine; D014425:Turpentine