title: The isolation and characterisation of a putative adipocyte precursor cell type from the white adipose tissue of the chicken (Gallus domesticus).
abstract: The conditions of isolation and culture of a chicken adipose stromal-derived cell strain are described and compared with chicken lung fibroblasts in vitro. The stromal cells accumulated intracellular lipid during the post-confluency culture period, this being by contrast with lung fibroblasts. Much higher levels of intracellular lipid were accumulated by the stromal cells when whole chicken serum or chicken plasma lipoproteins were added to the culture medium. Lipoprotein lipase activity emerged in stromal cells maintained post confluency. This activity was absent from pre-confluent stromal cells and pre- and post-confluent fibroblasts. The incorporation of 14C-acetate, 3H-oleic acid and 14C-glucose into lipids by the stromal cells exhibited a pattern compatible with a concerted shift in the metabolism of the cells towards lipid storage, particularly in the form of triacylglycerols derived from exogenous fatty acids. It is proposed that, in common with the mammalian species previously studied, the white adipose tissue of the chicken (Gallus domesticus) contains a cell type with properties which allow its preliminary identification as an adipocyte precursor cell capable of adipose conversion in vitro. The confirmation of this proposition is amenable to further investigation.
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D002645:Chickens / Q000033:anatomy & histology*; D005260:Female; D005947:Glucose / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D000085:Acetates; D008055:Lipids; D009829:Oleic Acids; D019301:Oleic Acid; D008071:Lipoprotein Lipase; D005947:Glucose; D019342:Acetic Acid