Enkephalin is liberated from metorphamide and dynorphin A1-8 by endo-oligopeptidase A, but not by metalloendopeptidase EC 3.4.24.15.
It has been previously reported that both the cysteinyl-endo-oligopeptidase A and the metalloendopeptidase EC 3.4.24.15 are able to generate enkephalin from a number of enkephalin-containing peptides, including dynorphin A1-8. The present study shows that only endo-oligopeptidase A is able to generate [Leu5]enkephalin and [Met5]enkephalin from dynorphin A1-8 and from metorphamide respectively. It is also shown that endo-oligopeptidase A neither hydrolyses the specific EC 3.4.24.15 substrate alpha-N-benzoyl-Gly-Ala-Ala-Phe p-aminobenzoate, nor is inhibited by the specific EC 3.4.24.15 inhibitor N-[1(RS)-carboxy-2-phenylethyl]-alpha-Ala-Ala-Phe p-aminobenzoate.
D011232:Chemical Precipitation; D003546:Cysteine Endopeptidases / Q000276:immunology / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors; D004399:Dynorphins / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*
D015853:Cysteine Proteinase Inhibitors; D004745:Enkephalins; D010446:Peptide Fragments; D004744:Enkephalin, Methionine; D004399:Dynorphins; C026770:dynorphin (1-8); C039700:adrenorphin; D003546:Cysteine Endopeptidases; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase