Determination of the HLA-DR profile of an HLA class II negative carcinoma cell line by restriction fragment length polymorphism (RFLP) analysis.
The human colonic adenocarcinoma cell line HT-29 does not normally express HLA class II molecules. By restriction fragment length polymorphism (RFLP) analysis of DNA with a DR beta-probe, we analysed the genomic DR beta polymorphism of this cell-line, and compared it with the RFLP patterns seen in DNA from a reference panel of different DR homozygous and heterozygous cells. The HT-29 cell-line expressed DR beta fragments similar to the sum of the fragments expressed by DR4 and DR7 homozygous cells. The DR4 and DR7 haplotypes of HT-29 was further confirmed by comparing the RFLP patterns of four DR4/7 heterozygotes with that of HT-29. Furthermore, the HT-29 cell line expressed a Hind III 9.3 kb fragment previously found to be strongly associated to DRw53. Following treatment with gamma-interferon, the HT-29 cells could be induced to express class II molecules. Serological typing revealed the presence of the DR4, DR7 and DRw53 antigenic determinants.
D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics / Q000276:immunology*; D004273:DNA, Neoplasm / Q000032:analysis; D006681:HLA-D Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D012150:Polymorphism, Restriction Fragment Length
D004273:DNA, Neoplasm; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D007371:Interferon-gamma