T11TS, the cell surface molecule binding to the "erythrocyte receptor" of T lymphocytes: cellular distribution, purification to homogeneity and biochemical properties.
T11 target structure (T11TS) is a sheep cell surface glycoprotein that binds to the E receptor of human and sheep T lymphocytes. Here we report that T11TS has a broad tissue distribution, including mature and immature hematopoietic cells, vascular endothelium and smooth muscle. The density of T11TS expression was determined by Scatchard analysis with radiolabeled anti-T11TS monoclonal antibody. Red blood cells bound 10,000, and leukocytes bound 4000 to 23,000 antibody molecules per cell. T11TS was purified to homogeneity by immune-affinity and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and some of its biochemical properties were determined. T11TS is an acidic (pI 4.5) membrane glycoprotein that binds to concanavalin A. It has 2 or 3 N-glycosidically linked carbohydrate side chains of the mature phenotype, no O-linked sugars, and an apparent mol. mass of 42 kDa (glycosylated) and 32 kDa (deglycosylated). The anti-T11TS monoclonal antibody L180/1, which blocks binding of sheep red blood cells to CD2, recognizes a protein determinant on T11TS. These findings are discussed with respect to the possible function of the CD2-T11TS system as a set of complementary cell interaction molecules involved in T cell activation.
D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000378:metabolism; D018801:CD2 Antigens; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002448:Cell Adhesion; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000494:pharmacology; D004912:Erythrocytes / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007962:Leukocytes / Q000032:analysis; D016169:Lymphocyte Function-Associated Antigen-1; D008565:Membrane Proteins / Q000032:analysis*; D011869:Radioligand Assay; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D018801:CD2 Antigens; D002352:Carrier Proteins; D006023:Glycoproteins; D016169:Lymphocyte Function-Associated Antigen-1; D008565:Membrane Proteins; D003208:Concanavalin A