Thermal characterization and transmitter analysis of single units in the preoptic and anterior hypothalamus of conscious ducks.
With a multibarrel assembly combining one carbon fiber micropipette as recording electrode and 6 pipettes for microiontophoretic application of drugs, the activity of neurons in the preoptic and anterior hypothalamic (POAH) region was extracellularly recorded in situ in conscious ducks implanted chronically with a device permitting hypothalamic thermal stimulation. Among 355 neurons 17% were identified as warm-responsive (warm units) and 20% as cold-responsive (cold units). In 58 warm and 56 cold units control discharge rates at 40 degrees C local temperature (F40) and temperature coefficients (delta F/delta T) were determined and presented as means +/- SEM. The F40 values of warm units (35.2 +/- 2.3 Imp . s-1) were significantly higher than of cold units (16.3 +/- 1.8 Imp . s-1). The delta F/delta T values (+1.77 +/- 0.15 and -1.77 +/- 0.19 Imp . s-1 . degree C-1) of warm and cold units were not different in absolute terms. In pilot experiments either activation or inhibition by lowering whole-body temperature was observed in both warm and cold units. Microiontophoretic application of one or more of the amines acetylcholine (ACh), 5-hydroxytryptamine (5-HT), and noradrenaline (NA) to warm and cold units revealed differences in their responsiveness to ACh, which more consistently stimulated cold units. NA inhibited the majority of warm units; 5-HT stimulated the majority of cold units. In both warm and cold units NA and ACh differed in their actions, with the latter amine more consistently producing activation.
D000818:Animals; D007025:Anterior Hypothalamic Nucleus / Q000033:anatomy & histology*; D001341:Autonomic Nervous System / Q000187:drug effects; D001679:Biogenic Amines / Q000494:pharmacology; D003243:Consciousness; D004372:Ducks / Q000502:physiology*; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology; D018377:Neurotransmitter Agents / Q000502:physiology*; D011301:Preoptic Area / Q000033:anatomy & histology*; D013696:Temperature; D013814:Thermal Conductivity
D001679:Biogenic Amines; D018377:Neurotransmitter Agents