Experimental induction of abdominal tympany, abomasitis, and abomasal ulceration by intraruminal inoculation of Clostridium perfringens type A in neonatal calves.
The etiologic role of Clostridum perfringens type A in the acute abdominal syndrome characterized by abomasal and rumen tympany, abomasitis, and abomasal ulceration was investigated in neonatal calves. Eight calves, 4 to 12 days old, were inoculated intraruminally with toxigenic C perfringens type A. Before and after C perfringens inoculation, blood samples were collected from all calves for blood gas and serum biochemical analysis and for determination of serum copper concentration; ruminal fluid was obtained for isolation of C perfringens. Calves were monitored daily for clinical signs of the syndrome and, depending on the severity of clinical signs, they were either euthanatized or redosed within 4 to 7 days. After necropsy, specimens obtained from the abomasum and rumen for macroscopic and microscopic examination and for anaerobic bacteriologic culture were processed in routine manner. Intraruminal inoculation of C perfringens type A into healthy calves induced anorexia, depression, bloat, diarrhea, and in some calves, death. Serum copper concentration was within normal range. Necropsy revealed variable degrees of abomasitis, petechial and ecchymotic hemorrhages, and ulcers (ranging from pinpoint to nearly perforate) in the abomasum. Seven of those calves also had multiple trichobezoars in the rumen. These necropsy findings were not seen in calves (controls) given distilled H2O only. In affected calves, acute abdominal syndrome was unrelated to copper deficiency, and C perfringens type A given intraruminally was able to induce clinical signs similar to those of the naturally acquired disease.
D000006:Abdomen, Acute / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D000018:Abomasum; D000818:Animals; D001784:Blood Gas Analysis / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000473:pathology; D003015:Clostridium Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D003016:Clostridium perfringens; D003085:Colic / Q000209:etiology / Q000662:veterinary; D003300:Copper / Q000097:blood; D004064:Digestive System / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D008297:Male; D013272:Stomach Diseases / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D013276:Stomach Ulcer / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D013577:Syndrome / Q000662:veterinary
D003300:Copper