Chloride transport blockers inhibit the chloride-dependent glutamate binding to rat brain membranes.
The effects of a series of chloride transport blockers (ethacrynate, furosemide, torasemide, 4,4'-diisothiocyano-2,2'-disulfonic acid stilbene and diphenylcarboxylate) on Cl(-)-dependent L-[3H]glutamate (Glu) binding were tested in rat brain membranes, Cl-transport blockers inhibit the Ca2+/Cl(-)-induced increase in L-[3H]Glu binding, some of them without affecting the Ca2+/Cl(-)-independent L-[3H]Glu binding. Increasing the medium osmolarity by augmenting the sucrose concentration also inhibited the Ca2+/Cl(-)-induced increase in L-[3H]Glu binding. The effects of both sucrose and Cl-transport blockers were not additive, suggesting that they acted on the same type of mechanism. We recently suggested that L-[3H]Glu binding to brain membranes corresponds to Glu uptake in membrane vesicles. Therefore we propose that the Cl-transport blockers inhibit a Cl(-)-dependent Glu accumulation into these vesicles.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D002712:Chlorides / Q000502:physiology*; D004976:Ethacrynic Acid / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008566:Membranes / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013449:Sulfonamides / Q000494:pharmacology; D000077786:Torsemide
D001713:Biphenyl Compounds; D002712:Chlorides; D005971:Glutamates; D013449:Sulfonamides; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D018698:Glutamic Acid; D005665:Furosemide; D004976:Ethacrynic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D000077786:Torsemide