title: Corticosterone decreases 3H-glutamate binding in rat hippocampal formation.
abstract: The effect of in vivo corticosterone manipulation on binding sites for the excitatory neurotransmitter glutamate was determined using radioligand binding to rat brain cryostat sections and crude synaptic membranes. 3H-glutamate binding to regions of the dorsal hippocampal formation was significantly decreased in adrenalectomized animals following 5-10 days of corticosterone treatment. Corticosterone did not alter 3H-glutamate binding in several other brain regions. The loss in 3H-glutamate binding appeared to be due to a decrease in the maximal number of binding sites, with little change in binding affinity. Both chloride-dependent and chloride-independent 3H-glutamate binding sites were decreased by corticosterone treatment. results indicate that corticosterone can selectively alter binding sites for excitatory amino acids in hippocampal tissue.
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001345:Autoradiography; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D003345:Corticosterone / Q000494:pharmacology*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism; D014316:Tritium
chemical_list: D002712:Chlorides; D005971:Glutamates; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D014316:Tritium; D018698:Glutamic Acid; D003345:Corticosterone