Clostridium perfringens iota toxin ADP-ribosylates skeletal muscle actin in Arg-177.
Clostridium perfringens iota toxin ADP-ribosylates actin. Substrates of C. perfringens toxin are both non-muscle beta/gamma-actin and skeletal muscle actin. This finding suggests that C. perfringens iota ADP-ribosylates the same amino acid in skeletal muscle and non-muscle actin as does C. botulinum C2 toxin in non-muscle actin. Protein chemical analysis involving thermolysin cleavage on [32P]ADP-ribosylated actin or tryptic digestion followed by a secondary thermolysin cleavage of the radiolabelled fragments showed one major site of ADP-ribosylation. From its amino acid composition and sequence, the radiolabelled peptide was identified as peptide 175-177, locating the acceptor ADP-ribosyl amino acid as Arg-177.
D036002:ADP Ribose Transferases; D000199:Actins / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism*; D001792:Blood Platelets / Q000032:analysis; D001905:Botulinum Toxins / Q000378:metabolism; D003016:Clostridium perfringens / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000032:analysis*; D010446:Peptide Fragments / Q000378:metabolism; D011817:Rabbits; D013820:Thermolysin
D000199:Actins; D001427:Bacterial Toxins; D010446:Peptide Fragments; C050737:iota toxin, Clostridium perfringens; D000246:Adenosine Diphosphate Ribose; D001120:Arginine; D036002:ADP Ribose Transferases; D013820:Thermolysin; D001905:Botulinum Toxins