Isolation and characterization of an amphiphilic form of human intestinal aminopeptidase N.
An amphiphilic detergent-solubilized human adult intestinal microvillous aminopeptidase N (EC 3.4.11.2) was purified by immunoadsorbent technique using meconial aminopeptidase N antibodies. Its molecular and kinetic properties were investigated to confirm the differences previously observed from the meconial enzyme.
D000596:Amino Acids / Q000032:analysis; D000626:Aminopeptidases / Q000302:isolation & purification*; D018826:CD13 Antigens; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D007700:Kinetics; D008871:Microvilli / Q000201:enzymology; D008970:Molecular Weight
D000596:Amino Acids; D000626:Aminopeptidases; D018826:CD13 Antigens