Taxol induces the formation of unusual arrays of cellular microtubules in colchicine-pretreated J774.2 cells.
Taxol is an antimitotic agent with the unique ability to induce the formation of parallel arrays of microtubules in cells. We have studied the effects of taxol on microtubule organization in the cultured macrophage-like cell line, J774.2, and shown that this novel reorganization of cellular microtubules is both a concentration-dependent and time-dependent phenomenon. In this paper, we have examined in detail the unusual microtubule arrays induced by taxol in colchicine-pretreated cells. Interphase cells which are pretreated with the irreversible inhibitor, colchicine, and then treated with taxol form a single microtubule aster associated with the nucleus and numerous discrete sites of apparent microtubule nucleation scattered throughout the cytoplasm. One interesting possibility is that these structures represent nucleation sites for taxol-induced bundles, a result supporting the notion that taxol-induced microtubule arrays are organized assemblies at what are perhaps secondary organizing sites.
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D008264:Macrophages / Q000648:ultrastructure; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure*; D017239:Paclitaxel; D014404:Tubulin / Q000032:analysis
D000470:Alkaloids; D014404:Tubulin; D017239:Paclitaxel; D003078:Colchicine