Identification of dynorphin 1-8 in human placenta by mass spectrometry.
The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M + H) ion in the fast atom bombardment mode. This octa-peptide could be the natural ligand of the kappa opioid receptors present in the human placental villus tissue.
D002824:Chorionic Villi / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins / Q000032:analysis* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000032:analysis* / Q000302:isolation & purification; D010920:Placenta / Q000032:analysis*
D010446:Peptide Fragments; D004399:Dynorphins; C026770:dynorphin (1-8)