Effect of indomethacin on plasma levels of vitamin D metabolites, oestradiol and progesterone in rabbits during early pregnancy.
1. Inhibition of prostaglandin (PG) synthesis by indomethacin (Id) during early pregnancy in rabbits apparently disrupts the process of sex steroid production by the ovaries. 2. The role of PGs as mediators in steroidogenesis was tested by investigating the effect of Id alone or in combination with progesterone, with oestradiol and progesterone, or with a mixture of PGs, on plasma levels of 25-hydroxyvitamin D (25(OH)D) and 1,25-dihydroxyvitamin D (1,25(OH)2D) in rabbits at an early stage of pregnancy. 3. Id alone significantly reduced plasma levels of both 25(OH) D and 1,25(OH)2D. Treatment with Id in combination with either oestradiol and progesterone, or with a mixture of PGs, resulted in the restoration of plasma levels of both vitamin D metabolites as well as the restoration of plasma levels of progesterone, to their control values.
D000818:Animals; D001667:Binding, Competitive; D002112:Calcifediol / Q000096:biosynthesis / Q000097:blood; D002117:Calcitriol / Q000096:biosynthesis / Q000097:blood; D002118:Calcium / Q000097:blood; D004958:Estradiol / Q000096:biosynthesis / Q000097:blood; D005260:Female; D006887:Hydroxycholecalciferols / Q000096:biosynthesis / Q000097:blood*; D007213:Indomethacin / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D010758:Phosphorus / Q000097:blood; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis / Q000097:blood; D011453:Prostaglandins / Q000096:biosynthesis; D011485:Protein Binding; D011817:Rabbits; D011863:Radioimmunoassay; D013256:Steroids / Q000096:biosynthesis / Q000097:blood*
D006887:Hydroxycholecalciferols; D011453:Prostaglandins; D013256:Steroids; D010758:Phosphorus; D011374:Progesterone; D004958:Estradiol; D002117:Calcitriol; D002112:Calcifediol; D002118:Calcium; D007213:Indomethacin