Detection of a new Msp I restriction fragment length polymorphism in the apolipoprotein A-I gene.
We report the existence of a Msp I restriction fragment length polymorphism in the first intron of the apolipoprotein A-I gene that is different from the one described by Seilhamer et al. (DNA 3, 309 (1984)). Size comparison of the newly discovered Msp I fragment with a restriction map of the apolipoprotein A-I gene revealed that most likely the cutting site at the 5'-end of the normally seen 673 bp fragment is lost giving rise to the observed 719 bp Msp I fragment. Based on analyses of 136 DNAs of healthy and non-related caucasians the allelic frequency was determined to be 0.06. The observed Msp I genotype frequencies are in Hardy-Weinberg equilibrium.
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood / Q000235:genetics*; D004247:DNA / Q000032:analysis; D005260:Female; D005796:Genes; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D008075:Lipoproteins, HDL / Q000235:genetics*; D008297:Male; D010375:Pedigree; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL; D004247:DNA