Sulfasalazine and its metabolites attenuate respiratory burst of leukocytes--a possible mechanism of anti-inflammatory effects.
The effects of sulfasalazine and its metabolites, 5-amino salicylic acid and sulfapyridine on respiratory burst of polymorphonuclear leukocytes were investigated by determining not only luminol-dependent chemiluminescence for the detection of myeloperoxidase-mediated active oxidants, but also lucigenin-dependent chemiluminescence for the detection of superoxide anion. The present study showed that sulfasalazine, 5-amino salicylic acid and sulfapyridine attenuated active oxygen species from polymorphonuclear leukocytes at the concentrations comparable to clinical doses. Therefore, it was concluded that anti-inflammatory effects of sulfasalazine can be explained by the ability of sulfasalazine and its degraded compounds to scavenge active oxidants from polymorphonuclear leukocytes.
D000636:Aminosalicylic Acids / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D005609:Free Radicals; D006801:Humans; D008163:Luminescent Measurements; D019804:Mesalamine; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000097:blood*; D013427:Sulfapyridine / Q000494:pharmacology; D012460:Sulfasalazine / Q000494:pharmacology*
D000636:Aminosalicylic Acids; D000893:Anti-Inflammatory Agents; D005609:Free Radicals; D012460:Sulfasalazine; D019804:Mesalamine; D010100:Oxygen; D013427:Sulfapyridine