A simple and rapid characterization of influenza virus isolates by monoclonal antibodies in radioimmunoassay.
Radioimmunoassay (RIA) with infectious allantoic fluid directly bound to solid phase, suitable for detection and further characterization of influenza virus isolates, is described. This simple and rapid method was applied for description of isolates obtained from different regions of Czechoslovakia during influenza epidemic in 1983. The results confirmed that all 13 examined isolates represent influenza A viruses possessing H3 subtype haemagglutinin very similar to haemagglutinin of influenza viruses A/Bangkok/1/79 (H3N2), A/Belgium/2/81 (H3N2) and A/Philippines/2/82 (H3N2), respectively.
D000482:Allantois / Q000382:microbiology; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D002642:Chick Embryo; D003604:Czechoslovakia; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000145:classification / Q000276:immunology*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006389:Hemagglutinins, Viral