Increased expression of the putative growth factor receptor p185HER2 causes transformation and tumorigenesis of NIH 3T3 cells.
The HER2 gene encodes a cell-surface glycoprotein with extensive homology to the epidermal growth factor receptor. Recently it was found to be amplified in about 30% of primary human breast malignancies. In experiments designed to assess the role of the HER2 gene in oncogenesis, we found that overexpression of unaltered HER2 coding sequences in NIH 3T3 cells resulted in cellular transformation and tumorigenesis.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000209:etiology* / Q000235:genetics; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D005347:Fibroblasts / Q000473:pathology / Q000637:transplantation; D005786:Gene Expression Regulation; D006133:Growth Substances / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000209:etiology* / Q000235:genetics; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000502:physiology*; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000502:physiology; D014170:Transformation, Genetic; D014410:Tumor Stem Cell Assay
D004247:DNA; D004274:DNA, Recombinant; D006133:Growth Substances; D009856:Oncogene Proteins, Viral; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins