Loss of polymorphic restriction fragments of class I and class II MHC genes in a malignant melanoma.
DNAs from human malignant melanoma cells and autologous peripheral blood lymphocytes were evaluated by Southern blot analysis with probes for class I and II HLA genes. DNA of melanoma cells digested with PvuII, EcoRI and BglI and hybridized with a DR beta probe showed a loss of several fragments when compared with DNA from lymphocytes. The same DNAs were not distinguishable when hybridized with a DQ beta probe. Analysis of melanoma and autologous lymphocyte DNAs from the same patient with a class I cDNA, after digestion with several endonucleases, revealed a further loss of fragments in melanoma cells. Comparison of restriction fragment patterns of melanoma and lymphocytes with those of homozygous, serologically-typed cell lines indicated that melanoma cells have lost fragments diagnostic of DR2 and A1 antigens. A densitometric analysis of signals of several oncogenes in comparison with that of DR indicated that a duplication of the remaining DR allele had occurred in melanoma cells.
D004273:DNA, Neoplasm / Q000235:genetics; D006680:HLA Antigens / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008545:Melanoma / Q000235:genetics / Q000276:immunology*; D012150:Polymorphism, Restriction Fragment Length
D004273:DNA, Neoplasm; D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II