title: Secretagogues for pancreatic hormone release in the channel catfish (Ictalurus punctatus).
abstract: We investigated the effect of several potential carbohydrate secretagogues, amino acids, a ketoacid, and potassium chloride on insulin, glucagon, and somatostatin release from the in vitro perfused Brockmann body of channel catfish (Ictalurus punctatus). Mannose (15 mM) stimulated the release of insulin and somatostatin. Fructose (30 mM) induced only a small and transient release of somatostatin. Galactose (15 mM) was not a secretagogue. Likewise, glyceraldehyde failed to stimulate hormone release. Among the amino acids newly tested, alanine and leucine, and also alpha-ketoisocaproic acid were without effect. A high concentration of potassium (25 mEq/liter) induced a pronounced release of insulin and glucagon and a moderate release of somatostatin. In conclusion, a striking similarity exists between catfish and higher vertebrates in their pancreatic endocrine response to hexoses; on the other hand, the catfish Brockmann body appears to respond only to a few of the common stimuli of pancreatic hormone release in mammals.
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002397:Catfishes / Q000502:physiology*; D005632:Fructose / Q000494:pharmacology; D005934:Glucagon / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D005985:Glyceraldehyde / Q000494:pharmacology; D007059:Ictaluridae / Q000502:physiology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007651:Keto Acids / Q000494:pharmacology; D008358:Mannose / Q000494:pharmacology; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010187:Pancreatic Hormones / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D013004:Somatostatin / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D007328:Insulin; D007651:Keto Acids; D010187:Pancreatic Hormones; D005632:Fructose; D005985:Glyceraldehyde; D013004:Somatostatin; D011189:Potassium Chloride; C013082:alpha-ketoisocaproic acid; D005934:Glucagon; D005947:Glucose; D008358:Mannose