Adriamycin resistance in HL60 cells in the absence of detectable P-glycoprotein.
Previous studies have shown that the development of multi-drug resistance in cell lines treated with chemotherapeutic agents is closely associated with the overexpression of a 170-180 kilodalton surface membrane glycoprotein (P-glycoprotein). In the present study a monoclonal antibody against the P-glycoprotein was used to determine if this protein is overexpressed in multi-drug resistant HL60 cells. Using either indirect immunofluorescent staining or immunoblot analysis P-glycoprotein could not be detected in HL60 cells isolated for resistance to adriamycin. In contrast HL60 cells isolated for resistance to vincristine contain the P-glycoprotein and the amount of this material increases with increasing levels of resistance. These studies thus demonstrate adriamycin resistance in P-glycoprotein negative HL60 cells. Furthermore adriamycin and vincristine are found to have distinct effects in inducing overexpression of P-glycoprotein in the HL60 cell line. This information could be useful in the development of therapeutic strategies for the treatment of certain forms of cancer.
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D015470:Leukemia, Myeloid, Acute
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D006023:Glycoproteins; D004317:Doxorubicin