Localization of binding sites for purified Escherichia coli P fimbriae in the human kidney.
Binding sites in the human kidney for purified P fimbriae of pyelonephritogenic Escherichia coli were determined. The purified KS71A (F7(1)) fimbriae bound only to epithelial elements of the kidney, i.e., to the apical aspect of proximal and distal tubular cells, as well as to the apical and cytoplasmic sites of collecting ducts. In addition, binding was seen at the vascular endothelium throughout the kidney and at the parietal epithelium of the glomeruli. The binding was specifically inhibited by the receptor analog of E. coli P fimbriae, globotriose. The binding sites identified suggested a possible pathogenetic mechanism for the invasion of P-fimbriated bacteria into the renal parenchyma, as well as for their subsequent spread into the circulatory system.
D000230:Adenocarcinoma / Q000473:pathology; D001422:Bacterial Adhesion; D004926:Escherichia coli / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D010861:Fimbriae, Bacterial / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D007668:Kidney / Q000382:microbiology* / Q000473:pathology; D007680:Kidney Neoplasms / Q000473:pathology; D008297:Male