Creation of a chimeric oncogene: analysis of the biochemical and biological properties of v-erbB/src fusion polypeptide.
A novel gene was created that linked complementary portions of two different tyrosine kinase oncogenes: v-erB and v-src. The v-erbB/src chimera encoded a glycoprotein exhibiting the subcellular distribution of the v-erbB protein but containing the kinase catalytic domain of the v-src parent. Fibroblasts expressing the v-erbB/src gene product became transformed to an oncogenic state and closely resembled cells expressing the v-erbB parent oncogene. Our results indicated that v-erbB sequences can be functionally replaced by sequences derived from a different oncogene, v-src, and that important determinants of the transformed phenotype appear to be encoded in oncogene sequences distinct from those defining the kinase catalytic domain itself.
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D004900:Erythroblasts; D005347:Fibroblasts; D005796:Genes; D005813:Genes, Synthetic; D005814:Genes, Viral; D015688:Oncogene Protein pp60(v-src); D018739:Oncogene Proteins v-erbB; D009857:Oncogenes; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*; D012191:Retroviridae Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D018739:Oncogene Proteins v-erbB; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D012191:Retroviridae Proteins; D014764:Viral Proteins; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)