[Characteristics of the biological properties of a cell-free pertussis preparation].
The biological properties of Bordetella pertussis antigenic complex, obtained by a technologically simple method from the medium used for the cultivation of B. pertussis, were studied. The preparation was characterized by pronounced hemagglutinating activity, toxicity, histamine-sensitizing and leukocytosis-stimulating activity and produced a cytopathogenic effect on the culture of Chinese hamster ovary cells. The detoxified preparations showed pronounced protective activity in experiments on the active and passive protection of mice. The ED50 of the preparation was 0.146 microgram of protein. In the proposed human immunization dose containing 10 micrograms of protein the detoxified preparation showed no hemagglutinating, leukocytosis-stimulating or histamine-sensitizing activity and proved to be nontoxic in the weight loss test on mice.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D001886:Bordetella pertussis / Q000276:immunology; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D007114:Immunization; D007116:Immunization, Passive; D007928:Lethal Dose 50; D051379:Mice; D008808:Mice, Inbred CBA; D010567:Pertussis Vaccine / Q000276:immunology* / Q000302:isolation & purification / Q000633:toxicity; D010566:Virulence Factors, Bordetella / Q000633:toxicity
D000942:Antigens, Bacterial; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella
Kharakteristika biologicheskikh svoĭstv beskletochnogo kokliushnogo preparata.