Repair of potentially mutagenic damage, sensitive to anisotonic treatment, in actively growing Chinese hamster cells.
Using exponentially growing Chinese hamster V79 cells we studied the effect of anisotonic phosphate-buffered saline (PBS) treatment on the frequency of mutation following X-irradiation. Induction of mutants was studied at the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT). When cells were X-irradiated and immediately followed by 0.5 M NaCl/PBS treatment for 20 min at 37 degrees C, cell survival was significantly decreased and a concomitant increase in the number of mutants was observed, indicating that postirradiation treatment with anisotonic salt solution caused conversions of potentially lethal damage and potentially mutagenic damage to lethal and mutagenic damage, respectively. The repair kinetics of potentially mutagenic damage expressible by 0.5 M NaCl/PBS after 5.86 Gy of X-rays were similar to those of potentially lethal damage. The repair of both types of damage was essentially complete within 40 min after X-irradiation.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004260:DNA Repair; D066298:In Vitro Techniques; D009154:Mutation; D011831:Radiation Genetics
D003470:Culture Media