T cell independent Thy-1 allo-antibody response with the use of transgenic mice.
We have introduced a mouse Thy-1.1 gene into the germline of Thy-1.2 mice. The introduced gene was shown to be expressed at very high levels in thymocytes when compared with the endogenous gene. Transgenic thymocytes were shown to evoke a higher than normal primary anti-Thy-1.1 antibody response in plaque-forming cell (PFC) assays. This result suggests that a direct quantitative interaction of the Thy-1 antigen activates the B cell response.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D000955:Antigens, T-Independent / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D007518:Isoantibodies / Q000096:biosynthesis; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000276:immunology; D014018:Tissue Distribution; D014162:Transfection
D000954:Antigens, Surface; D000955:Antigens, T-Independent; D007518:Isoantibodies; D018800:Thy-1 Antigens