Improved procedure for measuring gamma-glutamyltransferase isoenzymes in serum.
In this electrophoretic procedure for measuring isoenzymes of gamma-glutamyltransferase, patterns obtained are highly reproducible and the analytical imprecision (CV) ranges from 1.10% to 6.17%. A cellulose acetate support is used, at 220 V for 40 min. Sharply resolved isoenzyme bands were made visible by fluorescence scattered light, formed by use of a coumarin derivative as donor substrate. Two bands were observed for sera from normal subjects; four bands were variably present in sera from patients with different hepatobiliary diseases. Detection of the latter was satisfactory, down to a total activity in serum of 8-10 U/L. Three of the pathological bands were associated with low- and (or) very-low-density lipoproteins, whereas a major fraction of one of the normal bands in cirrhotic sera precipitated with high-density lipoprotein. The bands in normal sera, and one of the abnormal bands, did not.
D001660:Biliary Tract Diseases / Q000201:enzymology*; D011232:Chemical Precipitation; D002779:Cholestasis / Q000201:enzymology; D003374:Coumarins / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008103:Liver Cirrhosis / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology*; D008113:Liver Neoplasms / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000097:blood*
D003374:Coumarins; D007527:Isoenzymes; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; C027245:7-(gamma-glutamyl)-4-methylcoumarylamide; D005723:gamma-Glutamyltransferase