Rapid DNA purification for restriction fragment length polymorphism analysis.
This paper describes a method for isolation of DNA from blood samples involving a rapid chemical disintegration of proteins with 8 M urea and with a minimum of exposure to phenol. The DNA is further desalted and purified on Sephadex G-25 prepacked disposable columns. DNA isolated in this way was pure enough to be immediately cleaved by restriction enzymes.
D001345:Autoradiography; D015139:Blotting, Southern; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D006801:Humans; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA; D004262:DNA Restriction Enzymes