Solid phase synthesis of somatostatin-28 II. A new biologically active octacosapeptide from anglerfish pancreatic islets.
Somatostatin-28 II, an octacosapeptide recently isolated from anglerfish pancreatic islets, was synthetized by the solid phase method along with its somatostatin-14 II and somatostatin-28 II-(1-12) corresponding domains. Homogeneity of the synthetic peptides was demonstrated by analytical RP-HPLC, thin layer chromatography and electrophoresis. The peptides were further characterized by amino acids analysis, fast atomic bombarding mass spectrometry and/or 252Cf plasma desorption mass spectrometry. Synthetic somatostatin-28 II and somatostatin-14 II displace equally well the potent agonist (Tyr0,D-Trp8)-somatostatin-14 from its specific binding sites on anterior pituitary cells membranes. Both peptides activate adenylate cyclase from dispersed rat anterior pituitary cells.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D053561:Somatostatin-28 / Q000031:analogs & derivatives
D000596:Amino Acids; D013004:Somatostatin; D053561:Somatostatin-28; C043024:somatostatin 14, Tyr(7)-Gly(10)-; C043025:somatostatin 28, Tyr(7)-Gly(10)-