Evaluation of anaerobic microdilution MIC results with the Prompt Inoculation System.
The Prompt Inoculation System (3M Co.) was compared with the overnight suspension inoculation procedure used with a broth microdilution anaerobic commercial system (Micro-Media Systems) for differences in MIC. MIC results from both suspension methods using six National Committee for Clinical Laboratory Standards-recommended quality control organisms were identical in 18 instances (75%) and within +/- 1 log2 dilution in 96% of the comparisons. Results with 45 anaerobic clinical isolates also compared satisfactorily; 83% of the results were identical and 97% were within +/- 1 log2 dilution. In addition, the direct (Prompt)-inoculated microdilution trays produced better growth and more valid MIC results; 92% of the clinical isolates produced MIC results versus 79% by the overnight suspension procedure.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D001439:Bacteroides / Q000187:drug effects; D002228:Carbenicillin / Q000494:pharmacology; D002440:Cefoxitin / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology; D003016:Clostridium perfringens / Q000187:drug effects; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010406:Penicillins / Q000494:pharmacology; D010460:Peptococcus / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D010406:Penicillins; D008795:Metronidazole; D002981:Clindamycin; D002701:Chloramphenicol; D002440:Cefoxitin; D013752:Tetracycline; D002228:Carbenicillin