title: Inhibitors of inosinate dehydrogenase.
abstract: Inosinate dehydrogenase (IMP-dehydrogenase) from Chinese Hamster Ovary cells was found to be inhibited by two types of inhibitors. One type of inhibitor binds competitively at IMP-binding site and other type at NAD-binding site of the enzyme. Some new inhibitors of both classes have been illustrated here and their inhibition constants and type of inhibition determined. NAD-analog of tiazofurin was found to bind with both the sites and this type of inhibitors were found to be non-competitive with IMP and NAD.
mesh_terms: D000495:Allosteric Site; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D007168:IMP Dehydrogenase / Q000037:antagonists & inhibitors*; D007658:Ketone Oxidoreductases / Q000037:antagonists & inhibitors*; D009243:NAD / Q000031:analogs & derivatives / Q000378:metabolism; D010053:Ovary; D011485:Protein Binding; D012254:Ribavirin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D012265:Ribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology
chemical_list: D012265:Ribonucleotides; D009243:NAD; D012254:Ribavirin; D007168:IMP Dehydrogenase; D007658:Ketone Oxidoreductases; C033706:tiazofurin