A new method for producing a specific and high titre antibody against glutamate using colloidal gold as a carrier.
Antiserum against the glutamate was developed using colloidal gold as a carrier for the antigen. Glutamate was bound directly to colloidal gold without using cross-linking reagents such as glutaraldehyde, formaldehyde, or carbodiimide. Repeated injection of the colloidal gold-glutamate complex into the rabbit yielded a specific, high titre antiserum. Specificity was verified by radioimmunoassay and histochemically with absorption controls. The antiserum immunohistochemically detected numerous glutamate-containing neurons in the rat brain.
D000818:Animals; D000917:Antibody Formation; D001921:Brain / Q000166:cytology*; D003429:Cross Reactions; D003432:Cross-Linking Reagents; D005971:Glutamates / Q000032:analysis* / Q000276:immunology; D018698:Glutamic Acid; D006046:Gold; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D008297:Male; D011817:Rabbits; D051381:Rats; D014316:Tritium
D003432:Cross-Linking Reagents; D005971:Glutamates; D007106:Immune Sera; D007202:Indicators and Reagents; D014316:Tritium; D018698:Glutamic Acid; D006046:Gold