title: Schwann cells and collagen synthesis in taxol-treated nerve crush. An electron microscopic study.
abstract: The effect of nerve crush on collagen synthesis in rat sciatic nerve was studied by electron microscope. The crushed nerves were treated with taxol which is known to increase the amount of cytoplasmic microtubules at the expense of other cell organelles such as rough endoplasmic reticulum and Golgi complexes. The results were compared to those seen in crushed nerves without taxol treatment. After the injury the amount of collagen fibrils increased at the site of the trauma in both groups when compared to intact controls. Thin (30 mn in diameter) collagen fibrils were often arranged closely to the Schwann cell surface and were connected to deep invaginations in areas where the basal lamina had lost its typical integrity. This was concluded to indicate a probable site of collagen secretion and it provides further evidence that an adult injured nerve Schwann cell is capable of synthesizing fibrous collagen. In taxol-treated nerves additional, abnormally close connection between thin microfibrils of about 10 nm and thin 20-30 nm collagen fibrils appeared in an end-to-end fashion. The microfibrils showed occasional collagenous transverse band like structures. The rough endoplasmic reticulum and Golgi complex play an important role in the posttranslational modifications of the procollagen molecule. Taxol-induced degeneration of cell organelles such as the Golgi complex, which is also essential in the secretion of proteins may thus lead to defective maturation of collagen and may explain partly the altered collagen fibril formation.
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D003094:Collagen / Q000096:biosynthesis*; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000648:ultrastructure; D009409:Nerve Crush; D017239:Paclitaxel; D010587:Phagocytosis; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000470:Alkaloids; D003094:Collagen; D017239:Paclitaxel