Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis.
We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.
D000682:Amyloid / Q000302:isolation & purification*; D000686:Amyloidosis / Q000378:metabolism*; D001927:Brain Diseases / Q000378:metabolism*; D019286:Endopeptidase K; D010450:Endopeptidases; D006801:Humans; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D018622:PrP 27-30 Protein
D000682:Amyloid; D009419:Nerve Tissue Proteins; D018622:PrP 27-30 Protein; D010450:Endopeptidases; D019286:Endopeptidase K