High-performance liquid chromatographic assay for azatadine in human urine.
A high-performance liquid chromatographic assay for the quantitative determination of azatadine and a base (1 M sodium hydroxide) hydrolyzable conjugate of azatadine in human urine has been developed. Reversed-phase separation of azatadine and the internal standard, 8-chloroazatadine, was accomplished on a 300 X 3.9 mm I.D. mu Bondapak CN column. Following liquid-liquid extraction from urine, azatadine was quantitatively determined by UV detection at 214 nm. No interferences were observed in the extracts obtained from drug-free urine. Detector response (peak area ratio) was linear from 10 to 2500 ng/ml. This method has been shown to provide accurate and precise determinations of the unchanged and hydrolyzed drug in human urine, following the twice daily oral administration (1-2 mg) of azatadine maleate.
D002851:Chromatography, High Pressure Liquid; D003533:Cyproheptadine / Q000031:analogs & derivatives* / Q000652:urine; D006634:Histamine H1 Antagonists / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D013696:Temperature
D006634:Histamine H1 Antagonists; D007202:Indicators and Reagents; D003533:Cyproheptadine; C006656:azatadine