2-Oxo-[14C]glutarate is taken up by glutamatergic nerve terminals in the rat striatum.
High affinity uptake of [14C]glutamate into rat striatal synaptosomes was reduced by 33% after bilateral cortical ablation. The lesion had no effect on striatal [14C]GABA uptake, but reduced 2-oxo-[14C]glutarate uptake by 67%. The results demonstrate the existence of a high-affinity uptake site for 2-oxoglutarate on glutamatergic nerve terminals and support the contention that this Krebs cycle intermediate may be used to replenish the neuronal pool of neurotransmitter glutamate. 2-Oxo-[14C]glutarate uptake may serve as a selective marker for glutamatergic neurones.
D000818:Animals; D002540:Cerebral Cortex / Q000502:physiology; D003342:Corpus Striatum / Q000378:metabolism*; D005971:Glutamates / Q000502:physiology*; D018698:Glutamic Acid; D007656:Ketoglutaric Acids / Q000378:metabolism*; D008297:Male; D009434:Neural Pathways / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism
D005971:Glutamates; D007656:Ketoglutaric Acids; D018698:Glutamic Acid