title: N-terminal amino acid sequence analysis indicates that isolated atrial amyloid is derived from atrial natriuretic peptide.
abstract: Isolated atrial amyloid, the most frequent senile cardiac amyloid type, was chemically analysed. Amyloid fibrils obtained from a patient (NIP) were extracted and the predominant low-molecular-weight polypeptide (approximately 3.5 kDa, designated ASc2 NIP) was isolated by size exclusion high performance liquid chromatography in 60% formic acid. N-Terminal amino acid sequence analysis of this polypeptide was identical to that of the atrial natriuretic peptide alpha-hANP for the first 12 residues determined.
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000682:Amyloid / Q000032:analysis* / Q000302:isolation & purification; D009320:Atrial Natriuretic Factor / Q000032:analysis*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D000682:Amyloid; C056928:amyloid fibril protein ASc2; D009320:Atrial Natriuretic Factor