Effect of human leukocyte interferon on HBsAg production in PLC/PRF/5 human hepatoma cell line.
We describe the effect of the natural human interferon alfa (HuIFN alfa) on HBsAg production in PLC/PRF/5 cells. Decreased HBsAg production was regularly demonstrated 48 hr after the treatment of cells with 3-300 IU of HuIFN alfa/ml; it lasted for further 24-48 hr depending from the amount of interferon (IFN) added. We found no differences in the sensitivity to HuIFN alfa of non-confluent cells as compared to cells which had reached confluency. The decrease of the extracellular HBsAg excretion seems to be due to inhibition of intracellular HBsAg formation.
D000998:Antiviral Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000382:microbiology / Q000473:pathology; D002460:Cell Line; D003864:Depression, Chemical; D005786:Gene Expression Regulation / Q000187:drug effects; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006515:Hepatitis B virus / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000382:microbiology / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology
D000998:Antiviral Agents; D006514:Hepatitis B Surface Antigens; D007370:Interferon Type I