Molecular cloning of nit-2, a regulatory gene required for nitrogen metabolite repression in Neurospora crassa.
We used an efficient sib-selection procedure to isolate a cosmid clone that complemented a mutated nit-2 gene of Neurospora crassa. Restriction fragment length polymorphism mapping indicated that the cosmid DNA insert was derived from linkage group IL, between 5S rDNA locus 12 and mt, the region of the N. crassa genome that contains nit-2. We conclude that the cosmid carries the nit-2 gene.
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D005800:Genes, Fungal; D005809:Genes, Regulator; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length
D009584:Nitrogen