title: Immunoelectron microscopic demonstration of pancreatic polypeptide in midgut epithelium of hematophagous dipterans.
abstract: Midguts of mosquitoes, Aedes aegypti and Anopheles stephensi, and of the tsetse fly, Glossina morsitans morsitans, as well as guinea pig pancreas, were prepared for electron microscopy by using low-temperature embedding in Lowicryl K4M. Rabbit antiserum to bovine pancreatic polypeptide (PP) crossreacted with secretory granules of pancreatic PP-producing cells and of the clear cells in mosquito gut. Rabbit antiserum to human somatostatin crossreacted with the control tissue, guinea pig pancreas D-cells, but not with the mosquito clear cells. None of the antisera used showed a distinct reaction with the endocrine-like cells of tsetse fly midgut. Positive reactions were revealed by gold as electron-dense marker. The gold particles were coated with protein A-gold or goat antibodies to rabbit immunoglobulin.
mesh_terms: D000330:Aedes / Q000378:metabolism*; D000818:Animals; D000852:Anopheles / Q000378:metabolism*; D004702:Endocrine Glands / Q000648:ultrastructure; D004848:Epithelium / Q000378:metabolism; D006046:Gold; D007158:Immunologic Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D008854:Microscopy, Electron / Q000379:methods; D010191:Pancreatic Polypeptide / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism; D014370:Tsetse Flies / Q000378:metabolism*
chemical_list: D013004:Somatostatin; D010191:Pancreatic Polypeptide; D006046:Gold