A soluble-factor(s) secreted by a human skin cancer cell line supports clonal growth of adult T-cell leukemia cells.
Leukemic cells from four out of eight patients with adult T-cell leukemia (ATL) were successfully grown by cocultivation with HSC-I cells, a human skin cancer cell line, in the presence of interleukin-2. Three of these four cultures of growing cells showed rearrangement of the T-cell receptor beta-chain gene like the original leukemic cells in vivo, and also showed conservation of the patterns of HTLV-I integration of the original leukemic cells in vivo. Cell-to-cell contact between HSC-I cells and leukemic cells was not necessary for growth of the leukemic cells. The results indicate that some soluble growth factor secreted by HSC-I cells and interleukin-2 are required for the in vitro growth of leukemic cells from some patients with adult T-cell leukemia.
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006800:Deltaretrovirus Infections / Q000378:metabolism / Q000473:pathology*; D006133:Growth Substances / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism / Q000502:physiology*; D010455:Peptides / Q000378:metabolism / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000302:isolation & purification; D012878:Skin Neoplasms / Q000378:metabolism*; D015291:Transforming Growth Factors
D006133:Growth Substances; D009363:Neoplasm Proteins; D010455:Peptides; D011948:Receptors, Antigen, T-Cell; D015291:Transforming Growth Factors