title: Alpha-neo-endorphin coexists with dynorphin-A(1-8) within intramurally lying perikarya of rat duodenum.
abstract: By the use of the immunofluorescent microscopic staining technique, adjacent serial sections through the rat duodenum were alternately stained with specific antisera directed to the opioid peptides alpha-neo-endorphin and dynorphin-A(1-8). alpha-Neo-endorphin immunoreactivity has been revealed exclusively within perikarya lying intramurally in the longitudinal muscle layer. These alpha-neo-endorphin and dynorphin-A(1-8) immunoreactive perikarya were large in diameter, round in shape, contained a large and round nucleus, and were recognized only occasionally there. alpha-Neo-endorphin immunoreactivity was coexistent with dynorphin-A(1-8)-positive material within these perikarya. Since no alpha-neo-endorphin material was detected within duodenal nerve fibres and terminals, it might be concluded that this peptide is further enzymatically cleaved to the opioid pentapeptide Leu-enkephalin during its axonal transport from intramural perikarya to nerve terminals and during its storage there.
mesh_terms: D000818:Animals; D004386:Duodenum / Q000166:cytology*; D004399:Dynorphins / Q000032:analysis*; D004723:Endorphins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008297:Male; D010446:Peptide Fragments / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004723:Endorphins; D007106:Immune Sera; D010446:Peptide Fragments; D011498:Protein Precursors; C025681:alpha-neoendorphin; D004399:Dynorphins; C026770:dynorphin (1-8)