[Enzyme systems of the substrate and cofactor supply of hyperlipogenesis in non-insulin-dependent diabetes].
Enzymatic systems of hepatic hyperlipogenesis supply by substrate (acetyl-CoA) and cofactors (NADPH and ATP) were studied in experiments on diabetic C57Bl/Ks J mice (db/db) that served as a model of non-insulin dependent diabetes. The rise in acetyl-CoA synthetase activity catalyzing the primary step of lipogenesis from acetate has been found, while pyruvate dehydrogenase complex activity did not differ from the control and ATP-citrate lyase activity was lowered. Hyperlipogenesis in non-insulin dependent diabetes was induced by the activation of cellular energy supply revealed in enhanced 2-oxoglutarate dehydrogenase activity and elevated ATP level, as well as changes in the activity ratio of NADPH supply and utilization and the rise in fructose-1,6-diphosphate, allosteric effector of fatty acid synthetase, which resulted in the increase of the enzyme activity and created wider potentials of NADPH utilization as a reducing equivalent in lipogenesis.
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000106:Acetate-CoA Ligase / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009249:NADP / Q000378:metabolism
D008055:Lipids; D009249:NADP; D000255:Adenosine Triphosphate; D007655:Ketoglutarate Dehydrogenase Complex; D001275:ATP Citrate (pro-S)-Lyase; D000106:Acetate-CoA Ligase
Fermentnye sistemy substratnogo i kofaktornogo obespecheniia giperlipogeneza pri insulinnezavisimom diabete.