Application of column switching in high-performance liquid chromatographic analysis of medroxalol in plasma.
An automated high-performance liquid chromatographic (HPLC) column-switching system is described for the analysis of medroxalol, a potential antihypertensive agent, in plasma. The HPLC system uses two six-port switching valves with a Corasil C18 short pre-column for an on-line sample clean-up and an SGE ODS analytical column for separation. Plasma samples were diluted with a phosphate buffer (pH 7.2) containing an internal standard and aliquots were injected directly on the HPLC system. The column-switching system was applicable to continuous analysis of hundreds of plasma samples since this technique provided very efficient on-line sample clean-up and regenerated the pre-column effectively. Results were in good agreement and the total analysis time was one third that of an alternative method.
D000319:Adrenergic beta-Antagonists / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004983:Ethanolamines / Q000097:blood*; D006801:Humans; D007700:Kinetics
D000319:Adrenergic beta-Antagonists; D004983:Ethanolamines; C024892:medroxalol