Parathyroid hormone-related protein purified from a human lung cancer cell line.
A protein with biological activities similar to parathyroid hormone (PTH) has been purified from serum-free culture medium obtained from a human lung cancer cell line (BEN). A major protein band of 18 kDa was obtained on NaDodSO4/polyacrylamide gels, with faint bands at 35 kDa and 67 kDa. Biological activity was associated only with the 18-kDa band. Amino acid sequence analysis of the material purified by HPLC revealed that 8 of the 16 residues were identical with those of human PTH. Antibody raised to a corresponding synthetic peptide recognized the PTH-related material but showed less than 1% cross-reactivity with human PTH amino-terminal peptides. BEN cells contained PTH DNA, but not PTH messenger RNA, indicating involvement of another gene. The purified PTH-related protein had a specific biological activity approximately equal to 6 times greater than that of bovine PTH(1-34). The PTH-related protein may have a role in the syndrome of humoral hypercalcemia of malignancy.
D000595:Amino Acid Sequence; D002294:Carcinoma, Squamous Cell / Q000032:analysis* / Q000150:complications; D002460:Cell Line; D003429:Cross Reactions; D000242:Cyclic AMP / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000032:analysis; D006729:Hormones, Ectopic / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D006934:Hypercalcemia / Q000209:etiology; D008175:Lung Neoplasms / Q000032:analysis* / Q000150:complications; D009363:Neoplasm Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D009384:Paraneoplastic Endocrine Syndromes / Q000209:etiology; D010281:Parathyroid Hormone / Q000276:immunology / Q000494:pharmacology; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments / Q000276:immunology / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011061:Poly A / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D019379:Teriparatide
D004273:DNA, Neoplasm; D006729:Hormones, Ectopic; D009363:Neoplasm Proteins; C470121:PTHLH protein, human; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D010455:Peptides; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D019379:Teriparatide; D011061:Poly A; D000242:Cyclic AMP