Biosynthesis of the neurofilament heavy subunit in Xenopus oocytes microinjected with rat brain poly(A)+ RNA.
In order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.
D000818:Animals; D001923:Brain Chemistry; D005260:Female; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000235:genetics*; D008845:Microinjections; D016900:Neurofilament Proteins; D011061:Poly A / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011985:Receptors, Serotonin / Q000096:biosynthesis / Q000235:genetics; D014982:Xenopus laevis
D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D011061:Poly A