Genetic analysis of autoimmune gld mice. I. Identification of a restriction fragment length polymorphism closely linked to the gld mutation within a conserved linkage group.
A linkage map of distal mouse chromosome 1 was generated using restriction fragment length polymorphism (RFLP) analysis of DNA prepared from 95 [C3H-gld/gld X Mus spretus)F1 X C3H-gld/gld] backcross mice. The gene order was: (centromere) C4bp, Ren-1,2, Ly-5, [At-3/gld], Apoa-2/Ly-17, Spna-1 (telomere). All mice expressing the phenotype of gld homozygotes were homozygous for the At-3 RFLP characteristic of C3H mice and none of the mice heterozygous for At-3 RFLPs had characteristics of gld homozygotes, demonstrating close linkage between these genes. The identification of an RFLP closely linked to the gld gene provides a starting point for the identification of a genetic defect that results in abnormal T cells and autoimmune disease.
D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D005260:Female; D005808:Genes, Recessive; D008040:Genetic Linkage; D008232:Lymphoproliferative Disorders / Q000235:genetics*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009154:Mutation; D010641:Phenotype; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic
D004262:DNA Restriction Enzymes