[Genetic transformation of somatic cells. XII. Mutations in human satellite DNA introduced into Chinese hamster cells].
Data are presented about detailed restriction mapping, hybridization and sequence analysis of plasmid pRT301 rescued into E. coli 25-30 cell divisions after transfection of Chinese hamster cells. This plasmid contains an insert of human satellite III fragment (HS3), which was replicated many times in mammalian cells, and we found multiple point mutations in this non-selectable fragment, mainly single inserts of GC base pair. Specificity of mutagenesis of HS3 induced by transfection and replication in Chinese hamster cells differs from mutagenesis specificity found by other authors for selectable genes.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004261:DNA Replication; D004276:DNA, Satellite / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length
D004276:DNA, Satellite
Geneticheskaia transformatsiia somaticheskikh kletok. XII. Mutatsii v satellitnoĭ DNK cheloveka, vvedennoĭ v kletki kitaĭskogo khomiachka.