Comparative in vivo and in vitro sister chromatid exchange studies in Chinese hamster bone marrow and spleen cells.
The sister chromatid exchange (SCE) assay in bone marrow and spleen cells of Chinese hamsters was used to evaluate the differences between in vivo and in vivo/in vitro (exposure of animals to chemical followed by culturing of cells) conditions. Cyclophosphamide, a mutagenic carcinogen, caused dose-related SCEs both in vivo and in vivo/in vitro. In the in vivo group, both bone marrow and spleen cells showed approximately a five-fold increase in SCEs over controls following 40 mg cyclophosphamide/kg treatment. The same dose, under in vivo/in vitro conditions, caused about three- and six-fold increases in SCEs over controls in bone marrow and spleen cells, respectively. While the extent of cyclophosphamide-induced SCEs (after subtraction of baseline level) in bone marrow is approximately the same under both conditions, the response was significantly higher in spleen cells in vivo/in vitro than in vivo. Under in vitro conditions, treatment of bone marrow and spleen primary cell cultures with a direct acting mutagen, trinitrofluorenone, caused significant dose-related increases in SCEs in both cell types in an equivalent manner. The replicative indices under these experimental conditions remained almost the same. Thus, this study indicates the potential usefulness of Chinese hamster bone marrow and spleen cells for in vivo and in vitro comparative studies with the same tissue to better assess the genotoxic hazard of chemicals.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005449:Fluorenes / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D008297:Male; D009152:Mutagenicity Tests; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
D005449:Fluorenes; D003520:Cyclophosphamide; C005644:2,4,7-trinitrofluorenone