Physiopathological studies on granulocyte-macrophage colony stimulating factor and multi colony stimulating factor producing leukemia, L8313, induced by irradiation of C3H mice.
Radiation-induced L8313 leukemia bearing mice (L8313 mice) had marked granulocytosis with splenomegaly. Hemopoietic stem cells and progenitors increased in the spleen but not in the bone marrow. Spleen conditioned-medium and serum from L8313 mice induced the formation of granulocyte-macrophage colonies (CFU-GM), erythroid bursts (BFU-E) and mixed colonies (CFU-Mix). Bone marrow conditioned medium did not show such activity. A cell line (STIL-3) was established from the spleen cells of L8313 mice. Surface marker analysis showed that the established cells were suppressor T cell. The cells produced IL-3 and GM-CSF in vitro, and induce essentially the same "leukemic" response in recipient mice. Inoculation of STIL-3 in diffusion chamber also induced leukemoid reaction, i.e. a marked granulocytosis with splenomegaly. Therefore, L8313 leukemia may be linked to an abnormality of growth and production of hemopoietic factors in hemopoietic regulatory cells.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000502:physiology; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000473:pathology; D007377:Interleukin-3 / Q000096:biosynthesis*; D007942:Leukemia, Experimental / Q000378:metabolism* / Q000473:pathology; D007953:Leukemia, Radiation-Induced / Q000378:metabolism* / Q000473:pathology; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013154:Spleen / Q000502:physiology; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D003470:Culture Media; D007377:Interleukin-3; D018800:Thy-1 Antigens