Conductometric monitoring of the amino acid oxidase reaction as a detector for high-performance liquid chromatography.
The deamination of L-amino acids by L-amino acid oxidase, which creates a change in the ionic strength of an HPLC eluent, is used to allow conductometric detection of L-amino acids. An example separation is given, and the conductometric method is compared to other methods of amino acid detection in the area of detection limits. Suggestions are also given for using this principle to create detectors based on other class selective enzymes.
D000596:Amino Acids / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003605:D-Amino-Acid Oxidase / Q000032:analysis*; D004553:Electric Conductivity; D004800:Enzymes, Immobilized; D006801:Humans
D000596:Amino Acids; D004800:Enzymes, Immobilized; D003605:D-Amino-Acid Oxidase