Peroxidation of mullet and rat liver lipids in vitro: effects of pyridine nucleotides, iron, incubation buffer, and xenobiotics.
1. Lipid peroxidation was observed in homogenates of mullet and rat livers and was affected by the specific conditions used for the in vitro assays. 2. Lipid peroxidation was altered by the buffer used in the assays and was dependent on the amount of Fe added to the assay. 3. Lipid peroxidation was stimulated by both NADH and NADPH and by the xenobiotics, CCL4 and DEM. 4. Lipid peroxidation was observed in microsomes of mullet livers and was inhibited by addition of cytosol.
D000818:Animals; D002021:Buffers; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism; D005399:Fishes / Q000378:metabolism*; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats
D002021:Buffers; D008054:Lipid Peroxides; D011725:Pyridines; D009249:NADP; D007501:Iron