Regulation by insulin of glucose metabolism in mammary gland of anaesthetized lactating rats. Stimulation of phosphofructokinase-1 by fructose 2,6-bisphosphate and activation of acetyl-CoA carboxylase.
The effect of insulin on glucose metabolism in mammary gland was studied by the euglycaemic/hyperinsulinaemic-clamp technique. Measurement of metabolite concentrations and enzyme activities in the mammary gland suggests two sites of action of insulin: phosphofructokinase-1 and acetyl-coA carboxylase. The increase in phosphofructokinase-1 activity could be linked to the 2-fold increase in fructose 2,6-bisphosphate concentration, since no change in maximal activity and in sensitivity of the enzyme toward fructose 6-phosphate was detected in vitro.
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D005635:Fructosediphosphates / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D005635:Fructosediphosphates; D007328:Insulin; D007527:Isoenzymes; C027652:fructose 2,6-diphosphate; D010732:Phosphofructokinase-1; D000103:Acetyl-CoA Carboxylase; D005947:Glucose