Measurement of concentrations of the histamine H1 receptor antagonist temelastine in biological fluids.
A high-performance liquid chromatographic analysis of temelastine in biological fluids with UV detection at 229 nm is presented. The method involves ether extraction followed by re-extraction into phosphoric acid and chromatography on a reversed-phase column. The method has a lower limit of detection of 0.05 mg L-1 and is adequate for use in pharmacokinetic studies following oral administration.
D001646:Bile / Q000032:analysis; D001826:Body Fluids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006634:Histamine H1 Antagonists / Q000032:analysis* / Q000097:blood; D006801:Humans; D007700:Kinetics; D011744:Pyrimidinones / Q000032:analysis* / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
D006634:Histamine H1 Antagonists; D011744:Pyrimidinones; C049054:temelastine