Isolation of dengue viruses by intracerebral inoculation of mosquito larvae.
The isolation of dengue viruses from clinical specimens has always posed a particularly difficult problem. The use of invertebrate cell cultures such as AP-61 and C6/36 has reduced the time required for definitive diagnosis to within a week. More recently, inoculation of adult mosquitoes has been used but it requires more than a week to reach a confirmed laboratory diagnosis. We describe a method using intracerebral inoculation of immobilized fourth instar of Toxorhynchites splendens larvae for the isolation of dengue viruses from clinical specimens which yields results within a few days following incubation at 32 degrees C.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D009033:Culicidae / Q000382:microbiology*; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007814:Larva; D013696:Temperature
D000956:Antigens, Viral