[Enzyme histochemical demonstration of sodium-dependent glutamate uptake in glutamatergic brain structures in the rat].
Using a special tetrazolium salt technique, a striking correlation was observed between Na+ concentration of the incubation medium and the activity of glutamate dehydrogenase (GDH) in glutamatergic neuropil areas of the hippocampal formation, cerebellum, and other brain regions. Na+ concentration of 130 to 150 mmol/l caused maximal formazan production. The histochemical enzyme reaction in neuronal perikarya as well as biochemically estimated GDH activity were found to be rather inhibited by such Na+ concentrations. The GDH catalyzed sodium dependent increase in formazan production is discussed to be a consequence of the sodium dependence of glutamate uptake in glutamatergic brain structures supplying the enzyme with substrate.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006651:Histocytochemistry; D008297:Male; D051381:Rats
D005971:Glutamates; D018698:Glutamic Acid
Enzymhistochemischer Nachweis der Na+-abhängigen Glutamat-Aufnahme in glutamatergen Hirnstrukturen der Ratte.