Different effects of 2,4-dinitrophenol on rat liver mitochondrial oxidation of various substrates: succinate and glutamate vs 3-hydroxybutyrate and glycerol 3-phosphate.
In homogenate and isolated mitochondria from the liver of male and female rats of Wistar and August strains, a higher uncoupled vs State 3 oxidation of succinate and glutamate and lower that of 3-hydroxybutyrate and glycerol 3-phosphate were observed. The optimal concentration of 2,4-dinitrophenol (DNP) was in the range 7-12 microM for 3-hydroxybutyrate oxidation and 50-100 microM for succinate or glutamate oxidation.
D019297:2,4-Dinitrophenol; D020155:3-Hydroxybutyric Acid; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D004140:Dinitrophenols / Q000494:pharmacology*; D005260:Female; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D005994:Glycerophosphates / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013386:Succinates / Q000378:metabolism*; D019802:Succinic Acid
D004140:Dinitrophenols; D005971:Glutamates; D005994:Glycerophosphates; D006885:Hydroxybutyrates; D013386:Succinates; D018698:Glutamic Acid; D000244:Adenosine Diphosphate; C029620:alpha-glycerophosphoric acid; D019802:Succinic Acid; D019297:2,4-Dinitrophenol; D020155:3-Hydroxybutyric Acid