Induction of protective immunity to experimental hepatic amoebic infection in hamsters.
Immunisation of hamsters with the Sephadex G-200 chromatographed Fraction-I of the crude amoebic extract afforded protection in 58% of the animals against an intrahepatic challenge with a virulent subline of axenic Entamoeba histolytica NIH-200 (V). The protected animals had variable levels of anti-FI antiamoebic antibodies, while none of the infected controls had such antibodies. Findings suggest that chromatographed FI of crude amoebic extract might function as a potent immunogen affording protection in amoebic infection.
D000818:Animals; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000276:immunology*; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000276:immunology* / Q000302:isolation & purification; D006386:Hemagglutination Tests; D007114:Immunization; D008099:Liver / Q000469:parasitology; D008101:Liver Abscess, Amebic / Q000276:immunology* / Q000469:parasitology; D008647:Mesocricetus
D000953:Antigens, Protozoan