A new method for screening anxiolytic drugs in rats.
In order to evaluate the anxiolytic action of drugs, a simple experimental procedure using a corridor-type runway was designed. In this apparatus, five food pellets were set in a row on a plastic platform. Rats with one day food-deprivation take a food pellet and then usually return to the start box. The time required to take 5 pellets (total time) and the number of returns were recorded. Diazepam (DZP) at 1-3.2 mg/kg and zopiclone (ZOP) at 10 mg/kg caused decreases in both parameters. These effects were blocked by the benzodiazepine receptor blocker, Ro15-1788, at 10 mg/kg. However, tracazolate failed to produce any change in both parameters. Haloperidol and imipramine prolonged the total time while reducing the number of returns. In contrast to DZP and ZOP, pentetrazol, well known to possess an anxiogenic effect, prolonged the total time. These results suggest that decreases in both the total time and the number of returns produced by DZP and ZOP may be related to their anxiolytic action which is mediated by a benzodiazepine receptor. Therefore, this procedure would be a simple and selective method for detecting benzodiazepine-type anxiolytics.
D000818:Animals; D014151:Anti-Anxiety Agents; D001007:Anxiety / Q000187:drug effects*; D053961:Azabicyclo Compounds; D003975:Diazepam / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005442:Flumazenil / Q000494:pharmacology; D008297:Male; D008960:Models, Psychological; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY
D014151:Anti-Anxiety Agents; D053961:Azabicyclo Compounds; D010879:Piperazines; C515050:zopiclone; D005442:Flumazenil; D003975:Diazepam