Expression of K channels in Xenopus laevis oocytes injected with poly(A+) mRNA from the insulin-secreting beta-cell line, HIT T15.
Two types of exogenous K channel were identified in Xenopus laevis oocytes injected with poly(A+) mRNA from the insulin-secreting cell line HIT T15. One of these was the ATP-regulated K channel (G channel) as evidenced by its conductance and inhibition by tolbutamide. The other resembled the Ca-activated K channel from beta-cells.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D005260:Female; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000502:physiology*; D011061:Poly A / Q000235:genetics*; D015221:Potassium Channels / Q000502:physiology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D014044:Tolbutamide / Q000494:pharmacology; D014982:Xenopus laevis
D007328:Insulin; D015221:Potassium Channels; D012333:RNA, Messenger; D011061:Poly A; D014044:Tolbutamide; D002118:Calcium