Automated high-performance liquid chromatographic method with column switching for the determination of neurotransmitters and related compounds, ascorbic acid and uric acid in tissue extracts.
An automated high-performance liquid chromatographic method with electrochemical and fluorimetric detection and on-line data evaluation is described for the simultaneous measurement of indoleaminergic and catecholaminergic neurotransmitters, some of their metabolites and precursors and ascorbic and uric acids. Deproteinized tissue extracts from the central nervous system or peripheral organs are injected without prior purification (recovery greater than 90%). A switching system enables the compounds to be passed as necessary through one, two or three reversed-phase columns, which are then eluted simultaneously (analysis time 25 min). Fifty samples per day can be analysed with a precision of 95% for neurotransmitters and about 90% for ascorbic and uric acids.
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis*; D001322:Autoanalysis; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D018377:Neurotransmitter Agents / Q000032:analysis*; D051381:Rats; D014020:Tissue Extracts / Q000032:analysis*; D014527:Uric Acid / Q000032:analysis*
D002395:Catecholamines; D018377:Neurotransmitter Agents; D014020:Tissue Extracts; D014527:Uric Acid; D001205:Ascorbic Acid