Calcium homeostasis of epithelial cells.
1. Cytosolic free Ca2+ is an important regulator of ion transport processes in epithelial cells. 2. Free Ca2+ concentration is regulated by a concerted action of Ca2+ transport systems in plasma membranes and intracellular organelles. 3. These transport systems were studied in intestinal and renal cortical cells with emphasis on the transport capacities and Ca2+ affinities. 4. Ca2+ accumulation by permeabilized cells was compared to Ca2+ uptake by isolated organelles and membrane fractions. 5. Effects induced by cell or organelle isolation methods and the influence of temperature and pH on Ca2+ transport capacities were studied.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D004848:Epithelium; D006706:Homeostasis; D007413:Intestinal Mucosa / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D007672:Kidney Cortex / Q000166:cytology; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000255:Adenosine Triphosphate; D002118:Calcium