All CD2-positive human lymphocytes rosette with sheep erythrocytes in the presence of polyethylene glycol.
The efficiency of rosette formation between human peripheral resting lymphocytes and sheep erythrocytes (E) is remarkably increased in the presence of 1-4% polyethylene glycol of MW 10,000 (PEG). In fact, all CD2 ('sheep E receptor')-positive lymphocytes formed rosettes. CD2 positivity was assayed by immunofluorescence staining with the monoclonal antibody (mAb) Leu 5b. PEG also induced autologous E rosetting which otherwise did not take place under the usual conditions. The specificity of the rosettes was assessed by blocking CD2 with mAb Leu 5b and by blocking the CD2 target structure (T11TS) on the sheep E with the mAb L180/1. The mAb Leu 5b abolished all rosette formation, but mAb L180/1 only abolished the sheep E rosettes as this mAb is specific for sheep E and does not cross-react with human E. The results indicate that sheep E in the presence of PEG specifically rosette with all CD2-positive lymphocytes.
D000943:Antigens, Differentiation / Q000276:immunology*; D018801:CD2 Antigens; D002469:Cell Separation / Q000379:methods; D006801:Humans; D011092:Polyethylene Glycols; D011971:Receptors, Immunologic / Q000276:immunology*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000943:Antigens, Differentiation; D018801:CD2 Antigens; D011971:Receptors, Immunologic; D011092:Polyethylene Glycols