[Use of the coagglutination reaction of yeast-like Candida maltosa fungi for detecting fimbriae in intestinal bacteria].
The capacity of nonpathogenic yeast-like C. maltosa strains to coagglutinate Escherichia coli has been studied. C. maltosa cells have also been shown to coagglutinate E. coli possessing mannose-sensitive adhesins in a wide range of their concentrations (5-140 bacterial cells per C. maltosa cell). Strains belonging to types CFA/I and CFA/II with fimbriae, similarly to their corresponding paired genetically related strains without these adhesins, are practically incapable of agglutinating C. maltosa cells, while strains K88 and B41 react with them. The reaction occurs at a concentration of 9.5-37.0 and 38.0-55.5 bacteria respectively per C. maltosa cell and is not inhibited by 1% d-mannose. The suggestion that C. maltosa cell surface glycoproteins contain not only receptors for E. coli fimbriae, type I, but also components similar in their structure to receptors specific to the mannose-resistant adhesins of strains K88, K99 and 41, has been confirmed by hemagglutination inhibition with C. maltosa surface antigens as inhibiting agents.
D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002175:Candida / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests / Q000379:methods; D008358:Mannose / Q000276:immunology
D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D008358:Mannose
Ispol'zovanie reaktsii koaggliutinatsii drozhzhepodobnykh gribov Candida maltosa dlia vyiavleniia fimbriĭ u kishechnykh bakteriĭ.