Identification of somatostatin-containing neurons in primary cultures of rat cerebral cortex.
The expression of somatostatin-like immunoreactivity was investigated in primary cultures of dissociated cerebral cortical tissue from the rat. A subpopulation of neurons in the cortical cultures exhibited intense staining for somatostatin. These somatostatin-immunoreactive neurons corresponded to 1.25% of the total neuronal population. Stained neurons were typically small with a soma size of 10-20 micron. The majority of somatostatin-containing cells had stellate and bipolar morphology, with the bipolar class predominating.
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D046508:Culture Techniques; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D013004:Somatostatin / Q000378:metabolism*
D010455:Peptides; C029993:somatostatin-like peptides; D013004:Somatostatin