Campylobacter pylori detected noninvasively by the 13C-urea breath test.
The high endogenous urease activity of Campylobacter pylori was exploited in a non-invasive test for the presence of this organism in the stomach. When 13C-urea was administered orally after a test meal, urea-derived 13CO2 appeared in the respiratory CO2 of infected individuals at a constant rate for greater than 100 min. The test was validated in 26 individuals who underwent both the 13C-urea breath test and endoscopic biopsy of the antral mucosa for culture and histological examination. Each positive breath test proved to be correlated with a positive culture or Warthin-Starry silver stain of a mucosal biopsy specimen, or both.
D000328:Adult; D001944:Breath Tests / Q000379:methods*; D002167:Campylobacter / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000175:diagnosis*; D002245:Carbon Dioxide / Q000032:analysis; D002247:Carbon Isotopes; D005069:Evaluation Studies as Topic; D005260:Female; D005756:Gastritis / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011706:Pyloric Antrum / Q000382:microbiology; D014508:Urea; D014510:Urease / Q000032:analysis
D002247:Carbon Isotopes; D002245:Carbon Dioxide; D014508:Urea; D014510:Urease