Hepatic biotransformation in lean and obese Wistar Kyoto rats: comparison to that in streptozotocin-pretreated Sprague-Dawley rats.
1. Phase I and phase II biotransformation was compared in streptozotocin-induced hypoinsulinemic (STZ) and genetic hyperinsulinemic (WKY-fatty) rats. 2. Total cytochrome P-450 concentrations were reduced in both STZ and WKY, whereas styrene oxide hydrolase and benzphetamine N-demethylase activities were normal in STZ and reduced in WKY. 3. UDP-glucuronosyltransferase activity was decreased toward testosterone and 1-naphthol in STZ and WKY, and was increased toward estrone in the obese female WKY. 4. Glutathione S-transferase activity was decreased in STZ toward 1-chloro-2,4-dinitrobenzene, ethacrynic acid and sulfobromophthalein, but was similar to that in normal rats for WKY.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003600:Cytosol; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004851:Epoxide Hydrolases / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D007700:Kinetics; D008024:Ligands / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009765:Obesity / Q000201:enzymology*; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D013045:Species Specificity
D008024:Ligands; D003577:Cytochrome P-450 Enzyme System; C019781:benzphetamine N-demethylase; D010089:Oxidoreductases, N-Demethylating; D014453:Glucuronosyltransferase; D004851:Epoxide Hydrolases; C022686:styrene oxide hydratase