Control of follicular maturation in the mouse by a nonsteroidal regulator from sheep follicular fluid.
Follicular fluid from sheep ovaries was gel-chromatographed on Sephadex G-100, and the retarded active fraction (Fr-IV, Kav = 1) was rechromatographed on G-25 (GF2). When injected into pseudopregnant mice, it did not affect corpus luteum function, but at the time of the termination of pseudopregnancy ovulation was suppressed. A dose of 20 micrograms GF2 when injected into cycling mice inhibited follicular maturation and ovulation. Plasma progesterone levels were also reduced, indicating the role of a nonsteroidal factor in follicular maturation, ovulation, and luteinization.
D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis; D005496:Follicular Atresia; D006131:Growth Inhibitors / Q000302:isolation & purification*; D036341:Intercellular Signaling Peptides and Proteins; D008183:Luteal Phase; D051379:Mice; D009865:Oocytes / Q000187:drug effects; D006080:Ovarian Follicle / Q000502:physiology*; D010455:Peptides / Q000302:isolation & purification*; D011374:Progesterone / Q000032:analysis; D011555:Pseudopregnancy / Q000503:physiopathology; D012756:Sheep
D006131:Growth Inhibitors; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C018355:intraovarian peptides; D011374:Progesterone; D005640:Follicle Stimulating Hormone