Comparison of CEA distribution in lesions and tumors of salivary glands as determined with monoclonal and polyclonal antibodies.
Immunohistochemical localization of carcinoembryonic antigen (CEA) with conventional antibody to CEA (anti-CEA), nonspecific crossreacting antigen (NCA)-absorbed polyclonal antibody to CEA (NCAa-CEA), and monoclonal antibody to CEA (Mono-CEA) have been compared in obstructive lesions and salivary gland tumors. Normal salivary glands gave strong staining of the luminal borders of acinar cells with anti-CEA, whereas no staining occurred with Mono-CEA. Obstructive lesions showed occasionally marked staining with anti-CEA in some acinar cells, but there was no reaction with Mono-CEA. Of 69 pleomorphic adenomas examined, 34 were positively stained with anti-CEA, 18 with NCAa-CEA and 8 with Mono-CEA along the luminal borders of the tumor cells. The frequency of positive staining of material within tubular lumina was similar with all three immunoreagents. Neoplastic cells were positive with Mono-CEA in only three cases, while eight cases were positive with NCAa-CEA and 11 cases with anti-CEA. In salivary gland tumors true CEA is be found mainly at the border of tumor cells, but the frequency of positive reactions is low.
D000235:Adenolymphoma / Q000032:analysis; D000236:Adenoma / Q000032:analysis; D000906:Antibodies / Q000276:immunology*; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D002277:Carcinoma / Q000032:analysis; D006801:Humans; D008199:Lymphadenitis / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000032:analysis*; D012469:Salivary Glands / Q000032:analysis*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen