Antibodies to polymerase proteins of influenza virus A/PR/8/34 (H1N1): comparison on the immunoreactivity with polymerase proteins of other influenza A and B virus strains.
The polymerase proteins (PB1, PB2, PA) of the influenza virus strain A/PR/8/34 (H1N1) were isolated from whole virion or ribonucleoprotein (RNP) fractions by electrophoresis on polyacrylamide gel and electroelution or Sepharose CL-6B chromatography in the presence of SDS. Antisera to polymerase proteins (P proteins) were raised in rabbits; the immunoglobulins (Ig) were purified by affinity chromatography. Characterization of the antibody fraction by Western blot analysis showed a highly monospecific reaction with the three polymerase proteins. Spot immunobinding assay was used to compare the immunoreactivity of the monospecific polymerase antibodies with the P proteins of other influenza A subtypes and influenza B strains, revealing high immunoreactivity with the components of all influenza A strains and only insignificant reactivity with the components of the influenza B strains tested.
D000818:Animals; D000914:Antibodies, Viral; D007120:Immunochemistry; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000145:classification / Q000201:enzymology* / Q000276:immunology; D012316:RNA Nucleotidyltransferases / Q000276:immunology*; D012324:RNA-Dependent RNA Polymerase / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D013045:Species Specificity; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral; D014764:Viral Proteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase