Corticotropin-releasing factor activates tyrosine hydroxylase in rat and mouse striatal homogenates.
Corticotropin-releasing factor (CRF) caused a concentration-dependent increase in dopamine (DA) synthesis measured in rat striatal synaptosomes. The concentrations of CRF producing half-maximal and maximal stimulation were 40 and 300 nM, respectively. The maximal effect corresponded to a 34% increase from the control level of DA synthesis. CRF 1 microM increased DA synthesis in synaptosomes of mouse striatum by 48%. Pretreatment of striatal synaptosomes with CRF produced a persistent activation of tyrosine hydroxylase assayed in extracts of lysed synaptosomes. These results suggest that CRF may play a modulatory role in striatal DA synthesis by acting on receptors located on DA terminals.
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000201:enzymology*; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D004298:Dopamine / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D014443:Tyrosine; D003346:Corticotropin-Releasing Hormone; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine