Inducing effects of chronic administration of isoproterenol on 1-acyl-sn-glycero-3-phosphate and 1-acyl-sn-glycero-3-phosphocholine acyltransferases in rat parotid salivary gland.
1. In rat parotid gland, chronic administration of isoproterenol caused significant increase of linoleic acid and decrease of arachidonic acid at the sn-2 position of phosphatidylcholine. 2. The activities of 1-acyl-sn-glycero-3-phosphate and 1-acyl-sn-glycero-3-phosphocholine acyltransferases were increased 3-8-fold and 2-fold, respectively, in the parotid gland microsomes of isoproterenol-treated rat. 3. Furthermore, the specificity of these two enzymes for various acyl-CoAs was also changed by administration of isoproterenol. 4. The alteration of unsaturated fatty acid composition at the sn-2 position of phosphatidylcholine was at least in part due to the change of activity and substrate specificity of lysophospholipid acyltransferases.
D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; D008243:1-Acylglycerophosphocholine O-Acyltransferase; D000217:Acyltransferases / Q000096:biosynthesis*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D007545:Isoproterenol / Q000008:administration & dosage*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000201:enzymology; D010306:Parotid Gland / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D001095:Arachidonic Acids; D008041:Linoleic Acids; D016718:Arachidonic Acid; D019787:Linoleic Acid; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase; D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; D007545:Isoproterenol