Morphological and enzymatic heterogeneity of suramin-induced lysosomal storage disease in some tissues of mice and rats.
Suramin-induced lysosomal storage disease reproduced in the rat was extended to the mouse with the attempt to characterize enzymatically and morphologically heterogeneous responses of various organs to the drug. Suramin administration strikingly decreased (3-6 days afterward) the activity of beta-glucuronidase in all tissues studied (kidney, liver, heart, and skeletal muscle). The enzymatic responses were small in the activities of beta-N-acetyl-glucosaminidase. The activity of arylsulfatase A decreased to a varying degree in mouse tissues, but in rats the activity increased in liver and skeletal muscle. The activity of cathepsin D increased in rat tissues. Suramin induced morphological changes characteristic to lysosomal storage diseases in kidney and liver but not in heart and skeletal muscle of both mice and rats. Kidney was appreciably more susceptible to suramin than liver. The occurrence of lysosomal accumulations, membranous lamellar inclusions, and granular material were most prominent in tubular cells of kidney and in Kupffer cells of liver. These cells also presented intensive Alcian blue staining. Interestingly, the enzymatic and morphological responses did not correlate with each other, which may reflect differences in the regulation of lysosomal functions in various cell types.
D000118:Acetylglucosaminidase / Q000032:analysis; D000818:Animals; D002239:Carbohydrate Metabolism, Inborn Errors / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D002402:Cathepsin D / Q000032:analysis; D002553:Cerebroside-Sulfatase / Q000032:analysis; D004195:Disease Models, Animal; D005966:Glucuronidase / Q000032:analysis; D007668:Kidney / Q000201:enzymology / Q000648:ultrastructure; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009132:Muscles / Q000201:enzymology / Q000648:ultrastructure; D009206:Myocardium / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D013498:Suramin; D013997:Time Factors
D013498:Suramin; D002553:Cerebroside-Sulfatase; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D002402:Cathepsin D