title: On the glutamate transport through cell envelope vesicles of Halobacterium halobium.
abstract: Glutamate uptake by envelope vesicles of Halobacterium halobium was measured. Previous authors showed that the glutamate uptake needs the illumination as well as Na+ gradient across the membrane. The latter is considered to be the driving force for the uptake. No satisfactory explanation for the necessity of the illumination has not been given. We found that in the absence of Cl- in the medium, only Na+ gradient was enough to induce the glutamate uptake, i.e. no illumination was needed. Glutamate uptake was measured with various strains of H. halobium. We found that the envelope vesicles prepared from strains containing no bacteriorhodopsin showed the glutamate uptake in the dark and in the presence of Cl- in the medium provided only that Na+ gradient is imposed.
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D003624:Darkness; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006217:Halobacterium / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D009861:Onium Compounds / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D012176:Retinoids / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D014320:Triphenylmethyl Compounds / Q000494:pharmacology
chemical_list: D002712:Chlorides; D005971:Glutamates; D009861:Onium Compounds; D012176:Retinoids; D014320:Triphenylmethyl Compounds; C011561:triphenylmethylphosphonium; D018698:Glutamic Acid; D012964:Sodium