Presence of a MgATP/ADP-dependent pp50 phosphatase in bovine brain coated vesicles.
Coated vesicles are involved in the intracellular transport of membrane proteins between a variety of membrane compartments in which they must be able to undergo repeated membrane fusion and fission. We previously described the presence of cyclic nucleotide- and Ca2+-independent protein kinase activity in bovine brain coated vesicles which specifically phosphorylated a unique Mr = 50,000 coated vesicle integral protein (pp50) on a threonine residue. We describe now the presence in bovine brain coated vesicles of the antagonistic enzymatic activity which dephosphorylates pp50. This phosphoprotein phosphatase occurs under two interconvertible active and inactive forms. The activation process needs the simultaneous presence of Mg2+ and ATP or ADP. Unchelated ATP, but not unchelated ADP, inactivates the pp50 phosphatase. The latter is associated with the vesicular core. MgADP activation of the pp50 phosphatase implicates a different mechanism which does not need a phosphorylated intermediate. Thus, the pp50 phosphatase might belong to a new phosphatase type distinct from the four other classes of well known protein phosphatases.
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000648:ultrastructure*; D002417:Cattle; D002746:Chlorpromazine / Q000494:pharmacology; D003034:Coated Pits, Cell-Membrane / Q000201:enzymology*; D003992:Dibucaine / Q000494:pharmacology; D011992:Endosomes / Q000201:enzymology*; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology; D013997:Time Factors; D014268:Trifluoperazine / Q000494:pharmacology; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D010710:Phosphates; D010750:Phosphoproteins; D014639:Vanadium; D014268:Trifluoperazine; D014638:Vanadates; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D012969:Sodium Fluoride; D010744:Phosphoric Monoester Hydrolases; D003992:Dibucaine; D002746:Chlorpromazine