title: Cryopreservation of human fetal adrenal medullary cells.
abstract: Cryopreservation of human fetal adrenomedullary cells was performed, after dissociation of the gland and purification of chromaffin aggregates, using 10% dymethyl sulfoxide as cryoprotectant. More than 90% of thawed cultured clusters appear morphologically intact, showing extensive neurite outgrowth and absence of appreciable cellular loss. Immunoreactivity for tyrosine hydroxylase and response to nerve growth factor were demonstrated. These findings indicate that human medullary cells maintain their distinctive functional characteristics after cryopreservation.
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology; D004121:Dimethyl Sulfoxide; D005333:Fetus; D005615:Freezing; D005865:Gestational Age; D006801:Humans; D009414:Nerve Growth Factors / Q000494:pharmacology; D014021:Tissue Preservation / Q000379:methods*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D009414:Nerve Growth Factors; D014446:Tyrosine 3-Monooxygenase; D004121:Dimethyl Sulfoxide