Expression of E10 antigen on functionally distinct human T-cell subpopulations: comparison with 3A1 defined subsets.
Using PWM-driven immunoglobulin synthesis, we studied the regulatory effects of the peripheral blood T-lymphocyte subpopulations defined by the E10 antigen. This previously described antigen (E10) is present on 60% of TPBL, of T4+, and of T8+ cells. The helper activity on PWM-driven B-cell differentiation appears to be highly increased in E10- T cells. This higher capacity does not apparently reflect a different susceptibility to suppressor influences as comparable results were obtained when such suppressor influences are minimized either by removal of T8+ cells from E10- and E10+ T cells, or by removal of monocytes from responding B-cell populations. In contrast, the relative function of T-cell subsets defined by the related antigen 3A1 are influenced by the presence of suppressor cells. It is only in the presence of both T8+ cells and monocytes that 3A1+ cells exhibit a higher inducer effect. Our results suggest that E10 and 3A1 antigens--although showing strong distribution homologies--define different regulatory T-cell populations.
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D008819:Mice, Nude; D010641:Phenotype; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D011043:Pokeweed Mitogens