Isolation and characterization of 4'-hydroxy T-2 toxin, a new metabolite of the trichothecene mycotoxin T-2.
Biotransformation of the trichothecene mycotoxin T-2 by the hepatic S-9 fraction prepared from phenobarbital-treated rats yielded a new metabolic product designated RLM-3. The metabolite was purified from the hepatic preparation using preparative HPLC. The structural analysis of RLM-3 was carried out using gas chromatography/mass spectrometry and 1H and 13C NMR. RLM-3 was identified as 4'-hydroxy T-2. The toxicity of RLM-3 in comparison to T-2 toxin and 3'-hydroxy T-2 was assessed using a rat skin bioassay technique. The metabolite 4'-hydroxy T-2 was shown to be deacylated at the C-4 position to yield 4'-hydroxy HT-2 when incubated with rat hepatic S-9 preparations.
D000818:Animals; D001711:Biotransformation; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012717:Sesquiterpenes / Q000302:isolation & purification* / Q000378:metabolism*; D012871:Skin Diseases / Q000139:chemically induced; D013347:Subcellular Fractions / Q000378:metabolism; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism* / Q000633:toxicity
D012717:Sesquiterpenes; C055008:4'-hydroxy T-2 toxin; D013605:T-2 Toxin