title: Hox-1.6: a mouse homeo-box-containing gene member of the Hox-1 complex.
abstract: Hox-1.6, a mouse homeo-box-containing gene member of the Hox-1 complex, is described. The Hox-1.6 homeo-box shows more divergence than the other members of the complex with the Drosophila Antennapedia-like homeo-box class. This previously undescribed gene was studied with respect to its transcription pattern and was found to be expressed during mouse fetal development in an intestine-specific manner in adults, and in tumours or cell types exhibiting early endodermal-like differentiation. The study of embryonic partial Hox-1.6 cDNA clones revealed structural features common to other Drosophila and vertebrate homeo-box-containing genes, but also indicated that Hox-1.6 transcripts might display splicing patterns more complex than those known for other vertebrate homeo-genes. One of these cDNA clones contains a rather short open reading frame which would encode a protein of approximately 14.5 kd. The use of this clone as a probe for S1 nuclease mapping confirmed that different Hox-1.6 transcripts were present both in embryonic total RNA and in embryonal carcinoma cell cytoplasmic RNA. These various transcripts are probably generated by an alternative splicing mechanism and may thus encode a set of related proteins.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003360:Cosmids; D004247:DNA / Q000032:analysis; D004330:Drosophila / Q000235:genetics; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005801:Genes, Homeobox; D014644:Genetic Variation; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D012326:RNA Splicing; D014158:Transcription, Genetic
chemical_list: D004247:DNA