title: Common site of mutation in the erbB gene of avian erythroblastosis virus mutants that are temperature sensitive for transformation.
abstract: The genome of the avian erythroblastosis virus temperature sensitive mutant ts34 was cloned from a cell line that was shown to contain a single integrated copy of the virus. The mutation was localized to the v-erbB gene by making chimeric viruses between the mutant genome and that of wildtype. Sequencing of the mutant v-erbB gene revealed a single amino acid change of a histidine to an aspartate residue at a position equivalent to amino-acid 826 of the human epidermal growth factor receptor. Interestingly this is the identical mutation to that recently reported for another temperature sensitive mutant ts167, indicating that this may be a hot spot for mutations in the v-erbB gene that give rise to ts transformation mutants. In addition, the different biological phenotypes of ts34 and ts167 are suggested to be due to an additional mutation in v-erbA in ts167.
mesh_terms: D001355:Alpharetrovirus / Q000235:genetics*; D000595:Amino Acid Sequence; D001354:Avian Leukosis Virus / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D018739:Oncogene Proteins v-erbB; D009856:Oncogene Proteins, Viral; D010957:Plasmids; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D018739:Oncogene Proteins v-erbB; D009856:Oncogene Proteins, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes