Localization of [3H]glutamate binding sites in rat adrenal medulla.
A significant activity of L-[3H]glutamic acid binding was detected in the membranous particulate preparations obtained from the rat adrenal glands. In vitro addition of sodium acetate (100 mM) resulted in a drastic enhancement of the binding to cerebral preparations, while inducing a significant inhibition of the adrenal binding. Quisqualic acid elicited a prominent suppression of the adrenal binding in a concentration-dependent manner to a slightly greater extent than the inhibition of cerebral binding. N-Methyl-D-aspartic acid exerted a significant stimulatory action on the adrenal binding without affecting the cerebral binding. The adrenal medullary part possessed more than 5-fold higher binding activity than that in cortical part. These results suggest the possible existence of [3H]glutamate binding sites in rat adrenal glands which are distinctly different from those in the central structures.
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002540:Cerebral Cortex / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D008297:Male; D016202:N-Methylaspartate; D009928:Organ Specificity; D010069:Oxadiazoles / Q000378:metabolism; D016318:Quisqualic Acid; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D005971:Glutamates; D010069:Oxadiazoles; D001224:Aspartic Acid; D018698:Glutamic Acid; D016202:N-Methylaspartate; D016318:Quisqualic Acid; D019342:Acetic Acid