Different electrophoretypes of human rotaviruses in Hungary.
Polyacrylamide gel electrophoresis of rotaviral double-stranded ribonucleic acid (RNA) extracted directly from faecal specimens collected in three different parts of Hungary was applied to characterize and distinguish 21 randomly selected viral isolates. This technique made it possible to define 7 different electrophoretypes. Of the isolates 11 exhibited an identical "long" electrophoretic migration pattern. "Short" RNA pattern was found in two cases, and one atypical rotavirus was also revealed. This is the first description of rotavirus RNA electrophoretypes in Hungary.
D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005759:Gastroenteritis / Q000382:microbiology; D006801:Humans; D006814:Hungary; D007223:Infant; D007231:Infant, Newborn; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000382:microbiology
D012330:RNA, Double-Stranded; D012367:RNA, Viral