title: Quisqualate and N-methyl-D-aspartate (NMDA) receptors in induction of hippocampal long-term facilitation using conditioning solution.
abstract: Brief electrical high-frequency stimulation (tetanus) via the synapses induces long-term potentiation (LTP) in hippocampal neurons. In order to elucidate how LTP is produced, we attempted to induce long-term facilitation (LTF) by perfusing a conditioning solution (CS) instead of the tetanus. A 5 min perfusion of hippocampal slices with a CS containing glutamate, K+ at high concentration and no Mg2+ resulted in the generation of LTF in CAl and dentate neurons. CS lacking one of these 3 factors failed to produce LTF. When the glutamate of the CS was substituted by both quisqualate and N-methyl-D-aspartate (NMDA), LTF was initiated. This indicates that both quisqualate and NMDA receptors play an important role in the induction of LTP.
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D006168:Guinea Pigs; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D009119:Muscle Contraction; D009994:Osmolar Concentration; D010477:Perfusion; D011188:Potassium / Q000494:pharmacology*; D018091:Receptors, AMPA; D011955:Receptors, Drug / Q000502:physiology*; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000502:physiology*; D012996:Solutions; D013997:Time Factors
chemical_list: D005971:Glutamates; D018091:Receptors, AMPA; D011955:Receptors, Drug; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D012996:Solutions; D018698:Glutamic Acid; D011188:Potassium