Alterations by peroxisome proliferators of acyl composition of hepatic phosphatidylcholine in rats, mice and guinea-pigs. Role of stearoyl-CoA desaturase.
Rats, mice and guinea-pigs were administered p-chlorophenoxyisobutyric acid (clofibric acid) or 2,2'-(decamethylenedithio)diethanol (tiadenol). The treatments of rats and mice with either clofibric acid or tiadenol increased markedly the activities of stearoyl-CoA desaturase, palmitoyl-CoA chain elongation, 1-acylglycerophosphate (1-acyl-GP) acyltransferase and 1-acylglycerophosphocholine (1-acyl-GPC) acyltransferase, but not 2-acylglycerophosphocholine (2-acyl-GPC) acyltransferase in liver microsomes. The treatment of guinea-pigs with clofibric acid did not cause any change in the activities of these enzymes. The treatment of guinea-pigs with tiadenol caused a slight, but significant, increase in the activities of 1-acyl-GP acyltransferase and 1-acyl-GPC acyltransferase. The treatment of rats and mice with either clofibric acid or tiadenol increased markedly the proportion of 18:1 and decreased greatly the proportion of 18:0 in liver microsomal phosphatidylcholine. However, there is a considerable difference in the effects of the two peroxisome proliferators on the composition of polyunsaturated fatty acids in phosphatidylcholine between rats and mice. The treatment of guinea-pigs with either of the two peroxisome proliferators caused no change in acyl composition of phosphatidylcholine. The possible role of stearoyl-CoA desaturation in the regulation of acyl composition of phosphatidylcholine was discussed.
D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D002994:Clofibrate / Q000031:analogs & derivatives*; D002995:Clofibric Acid / Q000494:pharmacology*; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005233:Fatty Alcohols / Q000494:pharmacology*; D006168:Guinea Pigs; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008830:Microbodies / Q000187:drug effects; D008862:Microsomes, Liver / Q000201:enzymology; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*
D000214:Acyl Coenzyme A; D005233:Fatty Alcohols; D010713:Phosphatidylcholines; D010171:Palmitoyl Coenzyme A; C004568:tiadenol; C017725:stearoyl-coenzyme A; D002995:Clofibric Acid; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; D000217:Acyltransferases; D002994:Clofibrate