L-antigen and active potassium transport in HK and LK red cells of Barbary sheep (Ammotragus lervia).
1. The potassium concentration in red cells of 21 Barbary sheep showed a bimodal distribution, with five animals of LK type (K+ conc. 30-45 mM) and 16 of HK type (K+ conc. 80-95 mM). 2. Evidence is presented that both Lp and Ll antigens are present on LK Barbary sheep red cells. 3. Active K+ transport in LK Barbary sheep red cells was stimulated 3-5 fold by sheep and goat anti-L. 4. Active K+ transport in HK Barbary sheep red cells was higher than in LK red cells. Five out of six HK animals tested showed no stimulation of active K+ transport with anti-L. One HK animal (2BA2) showed some stimulation of active K+ transport, and also absorbed some anti-L from antisera, suggesting that Lp antigen is present on these red cells. 5. Ouabain-sensitive ATPase in membranes from HK and LK Barbary sheep red cells showed kinetics characteristic of HK and LK membranes of domestic goats and sheep; the ATPase of LK Barbary sheep membranes sensitized with anti-L was stimulated 2-fold due to an alteration in the internal sodium and potassium affinities in favour of sodium.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D001789:Blood Group Antigens; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D007700:Kinetics; D010042:Ouabain / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D012418:Ruminants / Q000097:blood* / Q000235:genetics
D001789:Blood Group Antigens; D010042:Ouabain; D000251:Adenosine Triphosphatases; D011188:Potassium