Production of antibodies to palytoxin: neutralization of several biological properties of palytoxin.
Palytoxin stimulated arachidonic acid metabolism (in bovine aorta endothelial and smooth muscle cells, rat keratinocytes, porcine aorta endothelial cells and rat liver cells), hemolyzed rat erythrocytes and was lethal to mice when administered intraperitoneally. Serum from rabbits immunized with a conjugate in which palytoxin was covalently bound to bovine albumin through its free amino group neutralized these biologic activities of palytoxin. Ninety-nine per cent of the neutralizing activity of the immunized rabbit serum was removed after precipitation of the rabbit IgG with a goat anti-rabbit IgG.
D000178:Acrylamides; D000818:Animals; D000917:Antibody Formation; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002460:Cell Line; D003064:Cnidarian Venoms / Q000276:immunology* / Q000633:toxicity; D011464:Epoprostenol / Q000096:biosynthesis; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D011453:Prostaglandins / Q000096:biosynthesis; D011863:Radioimmunoassay; D051381:Rats; D000095702:Polyether Toxins
D000178:Acrylamides; D016718:Arachidonic Acid; D001095:Arachidonic Acids; D003064:Cnidarian Venoms; D011464:Epoprostenol; D011453:Prostaglandins; C010272:palytoxin; D000095702:Polyether Toxins