The one-cell mouse embryo: cell cycle-dependent radiosensitivity and development of chromosomal anomalies in postradiation cell cycles.
One-cell mouse embryos were irradiated with X-rays at different cell cycle stages. Examination of structural chromosomal anomalies and of micronucleus formation in postradiation mitoses and interphases demonstrated cell cycle-dependent radiosensitivities in the order: late G2 phase greater than G1 phase greater than S phase greater than early G2 phase greater than stage of decondensing nuclei. Comparison of the quality and quantity of chromosomal aberrations from the first to the third mitosis led to the conclusion that new chromosomal anomalies were formed in the course of postirradiation cell cycles. This hypothesis was supported by an increasing number of micronuclei from 24 to 48 h post-conception. In addition to structural chromosomal aberrations, radiation-induced chromosome loss was observed with a frequency that was obviously independent of the exposed cell cycle phase. Loss of acentric chromosome fragments and of single chromosomes contributed to the micronucleus formation.
D000818:Animals; D002453:Cell Cycle; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry; D007399:Interphase; D008677:Metaphase; D051379:Mice; D015162:Micronucleus Tests; D008938:Mitosis; D008940:Mitotic Index; D011836:Radiation Tolerance; D015053:Zygote / Q000528:radiation effects*
D004247:DNA