Use of enzyme-linked synthetic DNA in diagnosis of falciparum malaria.
Plasmodium falciparum contains a family of 21-base-long repetitive DNA sequences in its genome. A 21-base synthetic DNA oligomer, formerly labelled with phosphorus-32 for autoradiographic detection of P falciparum DNA, was covalently coupled to alkaline phosphatase for histochemical detection. The conjugate (PFR1-AP) detected purified P falciparum DNA with a sensitivity and specificity equal to that of 32P-labelled probes after 2-day exposures. PFR1-AP did not detect host DNA or DNA of other Plasmodium species. In African blood specimens PFR1-AP specifically detected P falciparum infections of 100 parasites/microliter. This sensitive, rapid, nonisotopic probe will allow more widespread use of DNA hybridisation in the diagnosis of malaria.
D000469:Alkaline Phosphatase; D000953:Antigens, Protozoan; D002352:Carrier Proteins; D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D007630:Kenya; D008288:Malaria / Q000175:diagnosis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010963:Plasmodium falciparum / Q000235:genetics*
D000953:Antigens, Protozoan; D002352:Carrier Proteins; D004247:DNA; D000469:Alkaline Phosphatase