Rapid drug haptenization procedure: application to gentamicin and quinidine.
A rapid and simple drug conjugation procedure which utilizes the heterobifunctional reagent N-hydroxysuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate is described. With this protocol two drugs of diverse structure and solubility have been successfully haptenized. Rabbits immunized with these conjugates yielded drug-specific antibodies as judged by a solid phase radioimmunoassay. Furthermore, using this technique, no cross-reactivity could be detected. Such haptens also elicited an immune response in mice thus further extending their potential for the production of drug specific monoclonal antibodies.
D000818:Animals; D002417:Cattle; D005839:Gentamicins / Q000032:analysis*; D006241:Haptens / Q000138:chemical synthesis; D007106:Immune Sera; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011802:Quinidine / Q000032:analysis*; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000032:analysis
D005839:Gentamicins; D006241:Haptens; D007106:Immune Sera; D004364:Pharmaceutical Preparations; D012710:Serum Albumin, Bovine; D011802:Quinidine