Inhibition of cellular proliferation by antisense oligodeoxynucleotides to PCNA cyclin.
The proliferating cell nuclear antigen (PCNA or cyclin) is a nuclear protein recently identified as a cofactor of DNA polymerase delta. When exponentially growing Balb/c3T3 cells are exposed to antisense oligodeoxynucleotides to PCNA, both DNA synthesis and mitosis are completely suppressed. A corresponding sense oligodeoxynucleotide has no inhibitory effects. These experiments indicate that PCNA (cyclin) is important in cellular DNA synthesis and in cell cycle progression.
D000818:Animals; D001324:Autoantigens / Q000235:genetics*; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003062:Codon; D004261:DNA Replication / Q000187:drug effects*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008938:Mitosis / Q000187:drug effects*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen
D001324:Autoantigens; D003062:Codon; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D018809:Proliferating Cell Nuclear Antigen