title: Somatostatin effects on gastric electrolytes and pepsin in dogs with various secretory stimuli.
abstract: To determine possible sites and mechanisms of action of somatostatin (SS) in gastric secretory mucosa, secretion of pepsin, H+, Cl-, Na+ and K+ was stimulated in conscious fistula dogs by i.v. infusion of bethanechol, pentagastrin and histamine in the absence and presence of SS-14. At low dose (0.5 micrograms or 300 pmol/kg/h), SS-14 potently inhibited H+ and pepsin stimulated by bethanechol (80 micrograms/kg/h) to less than 5% of control; it required 2 micrograms or 1200 pmol/kg/h of SS-14 to achieve similar inhibition of pentagastrin (1.5 micrograms/kg/h)-stimulated secretion. In both cases, gastric [K+] was depressed by SS-14 infusion and recovered before H+ and pepsin. Similar sensitivity to SS suggests a Ca++-dependent mechanism or pathway of stimulation by gastrin similar to that by cholinergic agonists. By contrast, histamine, which acts via cyclic AMP pathways, was not inhibited by a large dose of SS-14 (20 micrograms/kg/h). SS inhibition is thus agonist (or pathway)- rather than organ- or cell-specific.
mesh_terms: D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D018723:Bethanechol; D001626:Bethanechol Compounds / Q000494:pharmacology; D004285:Dogs; D004573:Electrolytes / Q000378:metabolism*; D005750:Gastric Juice / Q000032:analysis; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D005755:Gastrins / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D010418:Pentagastrin / Q000494:pharmacology; D010434:Pepsin A / Q000378:metabolism*; D013004:Somatostatin / Q000494:pharmacology*
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D001626:Bethanechol Compounds; D004573:Electrolytes; D005755:Gastrins; D018723:Bethanechol; D013004:Somatostatin; D006632:Histamine; D010434:Pepsin A; D010418:Pentagastrin