Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells.
Comparison by subtractive hybridization of mRNAs revealed a moderately abundant message in highly tumorigenic CHEF/16 cells present at very low levels in closely related nontumorigenic CHEF/18 cells. After cloning and sequencing the corresponding cDNA, computer comparison showed closest homology with the human connective tissue-activating peptide III (CTAP III). The human tumor cell cDNA hybridizing with the Chinese hamster clone was isolated, sequenced, and found to have closer similarity to the Chinese hamster gene than to CTAP III. Thus, the cloned cDNAs from Chinese hamster and human cells represent a different gene, named gro. Studies of its transcriptional regulation have shown that expression is tightly regulated by growth status in normal Chinese hamster and human cells and relaxed in the tumorigenic cells so far examined.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D006224:Cricetinae / Q000235:genetics*; D003412:Cricetulus / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics; D011061:Poly A / Q000096:biosynthesis*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014407:Tumor Cells, Cultured / Q000378:metabolism*
C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D004273:DNA, Neoplasm; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011061:Poly A; C009862:connective tissue-activating peptide; D004247:DNA