Binding and internalization of rat colonic mucins by the galactose/N-acetyl-D-galactosamine adherence lectin of Entamoeba histolytica.
Purified rat colonic mucins inhibit Entamoeba histolytica in vitro adherence to and lysis of colonic epithelial cells by binding to the amoebic galactose/N-acetyl-D-galactosamine (Gal/GalNAc)-inhibitable adherence lectin. We found that 125I-labeled mucins demonstrated saturable Gal-specific binding to E. histolytica trophozoites (strain HM1:IMSS), with 2.8 x 10(3) binding sites per amoeba and a dissociation constant of 8.20 x 10(-11) M-1. Inhibition of parasite protein synthesis completely abrogated mucin binding; elevation of amoebic vesicle pH with ammonium chloride (10 mM) had no effect. Surface-bound 125I-labeled mucins were rapidly internalized and released from amoebae without evidence of proteolytic degradation. Three avirulent HM1 clones contained immunoreactive Gal/GalNAc lectin molecules with high-affinity binding of 125I-labeled mucins but exhibited markedly reduced rates of uptake and exocytosis of bound mucins. High-affinity binding by the Gal/GalNAc adherence lectin was followed by rapid internalization and eventual release of the colonic mucins. Additionally, defects in lectin surface expression and endocytosis were found in the avirulent clones.
D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004748:Entamoeba histolytica / Q000378:metabolism* / Q000472:pathogenicity; D005089:Exocytosis; D037161:Galectins; D006388:Hemagglutinins / Q000378:metabolism*; D008297:Male; D009077:Mucins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D037161:Galectins; D006388:Hemagglutinins; D009077:Mucins; D003513:Cycloheximide