title: Coated pits and vesicles in the osteoclast.
abstract: The osteoclast is a cell with a phagocytic ability not dissimilar to the macrophage. Nevertheless, the mechanisms by which it resorbs bone are poorly understood. The aim of this study was to examine the distribution of coated membrane structures in the osteoclast in order to gain further information about endocytosis in this cell. Osteoclasts around the developing tooth germs of young rats were examined using transmission electron microscopy, and immunocytochemistry. Results showed that previously described coated membrane structures within the ruffled border do not appear to be associated with coated pits or vesicles. Coated pits were, however, evident on the dorsal and lateral surfaces of the cell, particularly opposite the clear zone areas. Immunogold staining for clathrin confirmed that coated pits and vesicles are absent within both the clear zone and ruffled border areas, but present on the lateral and dorsal surfaces of the actively resorbing cell. It is suggested that clathrin-associated receptor-mediated endocytosis occurs along the lateral and dorsal surfaces of the osteoclast for the uptake of nutrients and macromolecules, while endocytosis of bone mineral by the ruffled border is mediated by a non-clathrin associated coated membrane structure.
mesh_terms: D000818:Animals; D002966:Clathrin / Q000032:analysis; D003034:Coated Pits, Cell-Membrane / Q000032:analysis / Q000648:ultrastructure*; D011992:Endosomes / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009940:Organoids / Q000032:analysis / Q000648:ultrastructure*; D010010:Osteoclasts / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002966:Clathrin