[Problems of selecting the reagent concentration in the solid-phase immunoenzyme method for determining antibody concentrations].
The problems of selecting the concentration of reagents with the aim of increasing the accuracy and sensitivity of reactions, as well as decreasing the consumption of reagents, are shown as exemplified by the antigen-antibody reaction of the first order. A new approach to the ELISA reaction is proposed, which makes it possible to present the totality of consecutive interactions of the reagents as block diagrams described by known mathematical expressions. The limitations of the linear dependence of the results of the reaction are shown, and the methodological recommendations for overcoming these limitations are given.
D000907:Antibodies, Bacterial / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D001886:Bordetella pertussis / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D007202:Indicators and Reagents; D008433:Mathematics
D000907:Antibodies, Bacterial; D007202:Indicators and Reagents
Problemy vybor kontsentratsii reagentov v tverdofaznom immunofermentnom metode pri opredelenii kontsentratsii antitel.