Lineage-specific expression of a human beta-globin gene in murine bone marrow transplant recipients reconstituted with retrovirus-transduced stem cells.
Recombinant retroviral genomes encoding a chromosomal human beta-globin gene have been used to transduce murine haematopoietic stem cells in vitro. After permanent engraftment of lethally irradiated recipients with the transduced cells, the human beta-globin gene is expressed at significant levels only within the erythroid lineage. These results indicate that it is possible to obtain stable expression of exogenous chromosomal DNA sequences introduced into mature haematopoietic cells in vivo via stem cell infection, and that human disorders of haemoglobin production may be more feasible candidates for somatic cell gene therapy than previously suspected.
D000818:Animals; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D005914:Globins / Q000096:biosynthesis / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D011828:Radiation Chimera; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics
D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins; D005914:Globins