Biochemical changes in the rat after chronic thioacetamide intoxication.
Male Wistar rats were given drinking water ad libitum with 0.075% thioacetamide (TAA) for 4 weeks. TAA treatment did not affect serum aminotransferase activities and total bilirubin content. The activities of 5'-nucleotidase, K+, Na+- and Mg2+-adenosine triphosphatases in liver plasma membrane preparations were strongly depressed, while that of gamma-glutamyl transferase was considerably increased. A decline in liver microsomal cytochrome P-450 and cytochrome b5 concentrations was also recorded. In contrast, the content of reduced glutathione in liver homogenate supernatant (9000 g) increased about 2-fold. As plasma membrane associated enzymes seem to be exclusively affected, the liver plasma membrane could be involved in the pathogenesis of the TAA-induced precirrhotic liver changes.
D000081:Acetamides / Q000633:toxicity*; D000284:Administration, Oral; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D056486:Chemical and Drug Induced Liver Injury; D008107:Liver Diseases / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D009708:Nucleotidases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013853:Thioacetamide / Q000633:toxicity*; D000637:Transaminases / Q000097:blood / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D000081:Acetamides; D013853:Thioacetamide; D005723:gamma-Glutamyltransferase; D000637:Transaminases; D009708:Nucleotidases; D000254:Sodium-Potassium-Exchanging ATPase