[A new method for the determination of the multiple molecular forms of gamma-glutamyltransferases after electrophoresis on cellulose acetate. Preliminary data].
What is described is a new, sensitive, simple and precise method for the visualization of the multiple molecular forms of the gamma-glutamyltransferase by means of electrophoresis on cellulose acetate in parallel with the serum protein pattern. The isoenzymatic pattern in 100 cases with a normal catalytic activity was mainly represented by two bands, one in the alpha 1 region and one in the alpha 2. Also examined was the serum of 103 cases having various hepatic biliary pathologies and a catalytic activity out of the reference interval, in an attempt to evaluate the diagnostic usefulness of the various isoenzyme patterns.
D004586:Electrophoresis / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008107:Liver Diseases / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D007527:Isoenzymes; D005723:gamma-Glutamyltransferase
Un nuovo metodo per la evidenziazione delle forme molecolari multiple della gamma-glutammiltransferasi (GGT) dopo elettroforesi su acetato di cellulosa. Dati preliminari.