Inhibition of lyso-PAF: acetyl-CoA acetyltransferase by salicylates and other compounds.
Diflunisal and benoxaprofen (20-100 microM) produced dose-dependent inhibitions of lyso-platelet activating factor: acetyl-CoA acetyltransferase in a lysate of rat pleural neutrophils. Salicylate and aspirin were inhibitory at concentrations of 1 mM and above. Nordihydroguaiaretic acid was a relatively potent inhibitor (I50 = 6 microM). Other compounds, including anti-inflammatory steroids, cyclooxygenase and 5-lipoxygenase inhibitors, appeared ineffective at relevant concentrations. Inhibitions by diflunisal and salicylate occurred at concentrations similar to expected plasma levels in humans at therapeutic doses. An inhibition of platelet-activating factor synthesis may contribute to the antiinflammatory, analgesic, or antipyretic actions of these compounds.
D000101:Acetyl-CoA C-Acetyltransferase / Q000037:antagonists & inhibitors*; D000123:Acetyltransferases / Q000037:antagonists & inhibitors*; D000818:Animals; D008297:Male; D009504:Neutrophils; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012459:Salicylates / Q000494:pharmacology*
C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D012459:Salicylates; D000123:Acetyltransferases; D000101:Acetyl-CoA C-Acetyltransferase