title: Somatostatin modulation of neurally mediated pepsinogen secretion from frog esophageal mucosa.
abstract: Frog esophageal mucosa contains peptic glands which are innervated by cholinergic neurons. When incubated in a medium containing 1.5 mM CaCl2, pepsinogen release from esophageal mucosa was increased by a high potassium concentration (55 mM KCl), 1,1-dimethyl-4-phenylpiperazinium (DMPP) or bethanechol. Whereas the response to bethanechol remained little changed, the response to high KCl concentrations or DMPP was abolished in the absence of Ca2+. The stimulatory effects of high KCl concentrations and DMPP were also eliminated by the presence of atropine or somatostatin. Furthermore, pepsinogen release in response to bethanechol was dose-dependently inhibited by somatostatin. Frog esophagus was found to contain somatostatin-like immunoreactivity, with a higher density at the end adjacent to the stomach. Chromatography of mucosa extract on Sephadex G-50 revealed a single peak of somatostatin-like immunoreactivity that coeluted with somatostatin-14. Immunohistochemical staining of the mucosa with peroxidase antiperoxidase technique demonstrated the presence of two varieties of somatostatin-like immunoreactivity-containing cells, one individually dispersed within the intercalated septa and the other in groups within the interlobular septa of the peptic glands. These results seem to indicate that somatostatin or somatostatin-like immunoreactivity may play a modulatory role in neurally mediated pepsinogen secretion in the frog esophagus.
mesh_terms: D000818:Animals; D001626:Bethanechol Compounds / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D004246:Dimethylphenylpiperazinium Iodide / Q000494:pharmacology; D004947:Esophagus / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009092:Mucous Membrane / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D010435:Pepsinogens / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011898:Ranidae; D013004:Somatostatin / Q000032:analysis / Q000494:pharmacology*
chemical_list: D001626:Bethanechol Compounds; D010435:Pepsinogens; D013004:Somatostatin; D004246:Dimethylphenylpiperazinium Iodide; D011188:Potassium; D002118:Calcium