Dibutyryl cAMP induction of type II 5'deiodinase activity in rat brain astrocytes in culture.
Dibutyryl cAMP treatment of cultured rat astrocytes results in the rapid appearance of T4 to T3 conversion catalyzed by type II iodothyronine 5'deiodinase, without altering other deiodinating pathways. Induction of enzyme activity was time-dependent with a lag period of 60 min, reaching plateau levels after 6-8 hours, and required continued synthesis of mRNA and new protein. Isoproterenol also induced T4 to T3 converting activity through beta-adrenergic receptor mediated interactions. These data suggest that dibutyryl cAMP stimulated astrocytes provide an excellent model for the study of the molecular and cellular events modulating T4 to T3 conversion in the brain.
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D001921:Brain / Q000201:enzymology*; D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D004790:Enzyme Induction; D007453:Iodide Peroxidase / Q000096:biosynthesis*; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors; D051381:Rats; D013974:Thyroxine / Q000378:metabolism; D013997:Time Factors; D014284:Triiodothyronine / Q000378:metabolism
D000318:Adrenergic beta-Agonists; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D014284:Triiodothyronine; D003994:Bucladesine; D007453:Iodide Peroxidase; D007545:Isoproterenol; D013974:Thyroxine