title: Thymocyte clones from 14-day mouse embryos. II. Transcription of T3 gamma gene may precede rearrangement of TcR delta and expression of T3 delta, T3 epsilon and T11 genes.
abstract: We have studied the state of TcR delta gene and the expression of T3 delta, T3 epsilon, T3 gamma and T11 (CD2) genes in the fetal thymocyte (FT) clones A2, G15, H5, E10, D5, H12, F1 and D11 obtained from a 14-day B10.BR mouse fetal thymus. The eight FT clones contain the TcR delta gene in the germ-line configuration as determined by Southern blot analysis. With the exception of clones E10 and D5, the other six FT clones express normal sized transcripts for T3 gamma gene and none of the eight FT clones produced detectable RNA transcripts for T3 delta, T3 epsilon and T11 genes as assessed by Northern blot analysis. Together with our previous work showing that all eight FT clones contain the TcR gamma and the TcR beta gene clusters in the germ-line configuration, the data indicate that the FT clones represent the earliest stage of T cell development identified within the thymus. Our results provide evidence that (a) the T3 gamma gene is the first of the genes that encode components of a TcR/T3 complex to be expressed in ontogeny within the thymus; (b) the T3 (delta, epsilon, gamma) genes are switched on asynchronously and their expression must be differentially regulated, and (c) the T11 gene product may not be involved in early stages of T cell development within the thymus.
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D018801:CD2 Antigens; D017252:CD3 Complex; D002999:Clone Cells; D015329:Gene Rearrangement, T-Lymphocyte; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D011971:Receptors, Immunologic / Q000235:genetics; D013601:T-Lymphocytes / Q000502:physiology*; D013950:Thymus Gland / Q000196:embryology*; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic