title: Complete nucleotide sequence and genetic organization of the bacteriocinogenic plasmid, pIP404, from Clostridium perfringens.
abstract: The complete nucleotide sequence of the bacteriocinogenic plasmid, pIP404, from Clostridium perfringens has been determined. The plasmid genome comprises 10,207 bp and has a dA + dT content of 75%. Functions have been tentatively assigned to 6 of the 10 open reading frames and an origin-like region of repeated sequence identified. The codon usage of this extremely dA + dT rich plasmid is highly unusual and displays a pronounced preference for codons with the lowest dG + dC content. Only one of the genes from pIP404 was expressed at a significant level in Escherichia coli, suggesting that the atypical codon usage could represent a major obstacle to heterologous gene expression.
mesh_terms: D001483:Base Sequence; D003016:Clostridium perfringens / Q000235:genetics*; D003062:Codon; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
chemical_list: D003062:Codon