title: The frayed N-terminal of the inhibitor protein of bovine mitochondrial F1-ATPase.
abstract: The N-terminal region of the bovine mitochondrial F1-ATPase inhibitor protein is frayed. In three independently isolated samples about 62% of chains start at glycine-1. A further 22% of chains start at residue 2, serine, and the remainder at residue 3, glutamic acid. No evidence can be found for alpha-N-formylglycine reported previously. The fraying may be a consequence of proteolytic processing of the precursor of the inhibitor protein during entry into the mitochondrion.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D013058:Mass Spectrometry; D008929:Mitochondria, Heart / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D000097795:ATPase Inhibitory Protein
chemical_list: D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D010446:Peptide Fragments; D011506:Proteins; D006180:Proton-Translocating ATPases; D000097795:ATPase Inhibitory Protein; C019913:clostripain