Urinary alanine aminopeptidase assay improved as result of multivariate response-surface analysis.
Optimization of determination of alanine aminopeptidase in urine by univariate study led to a method involving pretreatment of urine with Sephadex G50. Re-examination of the optimization by multivariate study led us to recommend higher optimal concentrations: 5.8 mmol/L for the substrate and 300 mmol/L for the Tris buffer. Under these new conditions, pretreatment of urine was no longer necessary and the assay could be completely automated.
D000626:Aminopeptidases / Q000652:urine*; D000704:Analysis of Variance; D002021:Buffers; D018826:CD13 Antigens; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods
D002021:Buffers; D000626:Aminopeptidases; D018826:CD13 Antigens