[Effect of Argiope lobata venom and its components on L[3H)glutamate binding with locust muscle membranes].
The Argiope lobata venom is shown to block synaptic potential at locust neuromuscular junctions and inhibit the high-affinity sodium independent L[3H]glutamate binding site in locust muscle membranes. The data obtained due to fractionation of venom evidence that it contains components which block synaptic potential and inhibit the binding of L[3H]glutamate (5 kDa and more) as well as components which block synaptic potential but do not inhibit the binding of L[3H]glutamate less than 5 kDa. These observations indicate that spider venom contains at least two components with different mechanism of action.
D000818:Animals; D001180:Arthropod Venoms / Q000494:pharmacology*; D001667:Binding, Competitive; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006110:Grasshoppers; D066298:In Vitro Techniques; D008970:Molecular Weight; D009469:Neuromuscular Junction / Q000187:drug effects / Q000378:metabolism*; D013111:Spider Venoms / Q000302:isolation & purification / Q000494:pharmacology*
D001180:Arthropod Venoms; D005971:Glutamates; D013111:Spider Venoms; D018698:Glutamic Acid
Deĭstvie iada pauka Argiope lobata i ego komponentov na sviazyvanie L[3H]glutamata s membranami myshts saranchi.