Autoradiographic localization of cerebellar excitatory amino acid binding sites in the mouse.
We have investigated the cellular localization of cerebellar excitatory amino acid binding sites in normal mice, in mice deficient in granule cells and, perhaps, stellate, basket and Golgi cells (granuloprival mice) and in mice lacking Purkinje cells. In the molecular layer of normal mouse cerebellum, the quisqualate-sensitive binding sites were the predominant type of excitatory amino acid receptor and there were relatively few N-methyl-D-aspartate or kainate-sensitive binding sites. The granule cell layer of normal mice contained a mixture of all 3 types, the N-methyl-D-aspartate-sensitive binding sites being predominant. In the molecular layer of granuloprival mice, the number of quisqualate-sensitive binding sites was increased to 214% of control (P less than 0.01), whereas N-methyl-D-aspartate-sensitive binding sites were decreased to 62% of control (P less than 0.001) and kainate-sensitive binding sites were unchanged. In the granule cell layer of these mice, quisqualate-sensitive binding sites were increased to 200% (P less than 0.01), N-methyl-D-aspartate-sensitive binding sites were decreased to 47% (P less than 0.001) and kainate-sensitive binding sites were decreased to 49% (P less than 0.01 of their respective control values. In the molecular layer of mice lacking Purkinje cells, quisqualate-sensitive binding sites were reduced to 29% (P less than 0.001) of control and N-methyl-D-aspartate-sensitive binding sites were unchanged. In the granule cell layer of these mice, neither quisqualate nor N-methyl-D-aspartate-sensitive binding sites were changed. These results suggest that (1) quisqualate-sensitive binding sites are located principally on dendrites of Purkinje cells and that they up-regulate after deafferentation; (2) N-methyl-D-aspartate-sensitive binding sites are located on granule cells and, perhaps, stellate, basket and Golgi cells, and (3) kainate binding sites are located on cell bodies of granule and, perhaps, Golgi cells.
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D001345:Autoradiography; D001665:Binding Sites; D002531:Cerebellum / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D007608:Kainic Acid / Q000378:metabolism*; D051379:Mice; D016202:N-Methylaspartate; D010069:Oxadiazoles / Q000378:metabolism*; D016318:Quisqualic Acid; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000378:metabolism*
D005971:Glutamates; D010069:Oxadiazoles; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D001224:Aspartic Acid; D018698:Glutamic Acid; D016202:N-Methylaspartate; D016318:Quisqualic Acid; D007608:Kainic Acid