title: Lymphoma-specific T- and B-cell responses suggest the involvement of HTLV-I in virus-non-productive lymphomas of a married couple.
abstract: In a married couple, a T-cell and a B-cell lymphoma occurred at the same time in the husband (FR) and wife (FE), respectively.  Serum antibodies of patient FE with less advanced tumor progression specifically recognized HTLV-I-related envelope precursor molecules of 66-68 kDa molecular mass on HTLV-I-infected T- and B-cell lines, but not on HTLV-II or HIV-infected cells.  In addition, in vivo-activated CD8+ T-cell lines (TCL) from this patient specifically lysed autologous B-lymphoma cells, T-lymphoma cells from the husband (FR), as well as the HTLV-I-transformed MT2 T-cell line.  All positive target cells shared an HLA-class-I cross-reactive determinant identified by the alloantiserum WER127. On a clonal level, the specificity of the cytotoxic T-cell response was unequivocally distinguishable from classical natural-killer-like cytotoxicity.  Results imply the involvement of a common inductive agent in the manifestation of malignant lymphoma in both patients (FR and FE).  Since antibodies from cases with classical HTLV-I-induced adult T-cell leukemias (ATLL) did not bind antigens on cells of either lymphoma (FR or FE) and active virus production was not demonstrable under various different conditions, these results argue against HTLV-1 itself being the transforming agent. However, humoral and cellular immune responses of one patient (FE), in addition to de novo HLA-class-1 antigen expression of both patients, are nonetheless consistent with the involvement of viral infections(s). These were responsble for the expression of HTLV-1-characteristic envelope determinants of the malignant progeny of respective T- and B-cell origin.
mesh_terms: D000368:Aged; D000914:Antibodies, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D015480:Deltaretrovirus Antibodies; D005260:Female; D006680:HLA Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D015490:HTLV-I Infections / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008297:Male; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology
chemical_list: D000914:Antibodies, Viral; D015480:Deltaretrovirus Antibodies; D006680:HLA Antigens; D006684:HLA-DR Antigens