Application of ELISA in the detection of goat pox antigen and antibodies.
Enzyme linked immunosorbent assay (ELISA) was standardized for detection of goat pox virus (GPV) antibodies and antigen using live and inactivated antigens and hyperimmune serum (HIS), convalescent, post-vaccinal, as also post-challenge sera. The ELISA was most sensitive in detection of antibody when compared with agar gel precipitation (AGP) and counterimmunoelectrophoresis (CIE) tests. There was no complete correlation between the antibody status of vaccinated goats and protective immunity as animals having detectable seroconversion were also solidly immune to virulent challenge. The application of ELISA in pox infections of goats has been discussed.
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006041:Goats; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D011212:Poxviridae / Q000276:immunology*; D011213:Poxviridae Infections / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral