Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate buffer.
A single buffer consisting primarily of potassium and glutamate is shown to be suitable for activity of almost all restriction endonucleases, DNA methylases, and many other DNA-modifying enzymes. The composition of this buffer differs substantially from buffers currently in use for these enzymes.
D002021:Buffers; D015254:DNA Modification Methylases / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000032:analysis*; D005971:Glutamates; D018698:Glutamic Acid; D013095:Spermidine
D002021:Buffers; D005971:Glutamates; D018698:Glutamic Acid; D015254:DNA Modification Methylases; D004262:DNA Restriction Enzymes; D013095:Spermidine