Correlation of myosin light chain phosphorylation and gamma aminobutyric acid receptors in Ascaris suum muscle.
Four different muscle relaxants were compared as to their effects on tonic Ascaris suum muscle to: physiological activity, intracellular response to regulatory light chain phosphorylation, and receptor pharmacology. Perfusion of Ascaris suum muscle with each relaxant, gamma-aminobutyric acid, avermectin, piperazine and chlorpromazine resulted in a dose-dependent loss of muscle tone. Comparison of intracellular effects indicated that gamma-aminobutyric acid and avermectin perfusion initiated an increase in the levels of dephosphorylated regulatory light chain while piperazine increased first site phosphorylation and chlorpromazine increased second site phosphorylation. Receptor pharmacology studies were used to compare the actions of gamma-aminobutyric acid and piperazine. It was found that bicuculline-methiodide and picrotoxin inhibited the effects of gamma-aminobutyric acid in a dose-dependent manner, but these drugs had no effect on muscle relaxation stimulated with piperazine.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology; D001200:Ascaris; D002746:Chlorpromazine / Q000494:pharmacology; D005260:Female; D007559:Ivermectin / Q000031:analogs & derivatives / Q000494:pharmacology; D007700:Kinetics; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D015879:Myosin Subfragments; D009218:Myosins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D010766:Phosphorylation; D000077489:Piperazine; D010879:Piperazines / Q000494:pharmacology; D011963:Receptors, GABA-A / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D000871:Anthelmintics; D015879:Myosin Subfragments; D010446:Peptide Fragments; D010879:Piperazines; D011963:Receptors, GABA-A; D000077489:Piperazine; D005680:gamma-Aminobutyric Acid; D007559:Ivermectin; C019264:avermectin; D009218:Myosins; D000109:Acetylcholine; D002746:Chlorpromazine