Biologically active, recombinant DNA in clathrin-coated vesicles isolated from rat livers after in vivo injection of liposome-encapsulated DNA.
DNA entrapped in liposomes containing lactosylceramide in the bilayers is found to be associated with clathrin-coated vesicles isolated from the rat livers after intravenous injection of these liposomes. The presence of the exogenous DNA in the coated vesicles was detected by Southern blotting. The amount of DNA present in the coated vesicles does not appear to vary up to 4 h after injection of the liposomes into the animals. The recognition of the lactosyl group present in the liposome by the galactose receptor present on the surface of the different liver cells may lead to their internalization in a way analogous to receptor-mediated endocytosis of various macromolecules. DNA present in the lumen of the coated vesicles is found to be biologically active as evidenced by its replication in bacterial cells and mouse fibroblasts.
D000818:Animals; D002460:Cell Line; D002966:Clathrin; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism*; D004247:DNA / Q000235:genetics*; D004261:DNA Replication; D004274:DNA, Recombinant / Q000302:isolation & purification / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D006028:Glycosphingolipids; D007790:Lactosylceramides; D008051:Lipid Bilayers / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D009857:Oncogenes; D010957:Plasmids; D051381:Rats
D002966:Clathrin; D004274:DNA, Recombinant; D006028:Glycosphingolipids; D007790:Lactosylceramides; D008051:Lipid Bilayers; D004247:DNA