Regulation of insulin mRNA abundance and adenylation: dependence on hormones and matrix substrata.
The insulin mRNA levels of rat insulinoma cell lines increased six- to eightfold, and the cells entered a transient state of growth arrest when they were cultured in serum-free, hormonally defined medium and on an extract of extracellular matrix derived from a basement membrane-secreting tumor line, EHS. Insulinoma cultures in growth arrest responded to glucose with a two- to threefold increase in insulin secretion associated with an insulin mRNA that contained a poly(A) tail that was 120 to 140 bases longer than normal.
D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D002460:Cell Line; D003470:Culture Media; D005109:Extracellular Matrix / Q000502:physiology; D005796:Genes; D005809:Genes, Regulator; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000235:genetics* / Q000378:metabolism; D000078790:Insulin Secretion; D007340:Insulinoma / Q000378:metabolism*; D007700:Kinetics; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects
D003470:Culture Media; D007328:Insulin; D012333:RNA, Messenger; D011061:Poly A; D005947:Glucose