Myeloproliferative disorder due to abnormal production of hematopoietic stimulators.
A new kind of myeloproliferative disorder (L-8313) has been discovered. It was transplantable into syngeneic mice with spleen cells. The mice showed hepato-splenomegaly with a marked leukocytosis and anemia 3 weeks after transplantation of L-8313 cells. The number of GM-CFU and CFU-S per spleen increased to more than 40 times normal. The results of chromosomal and PGK analysis demonstrated that these increased stem cells were of host origin. Both the culture medium of the spleen cells and the serum from L-8313 bearing mice showed high levels of IL-3, BPA and CSF. Consequently, hematopoietic cells of the host mice underwent remarkable proliferation in response to these stimulating factors when L-8313 cells were transplanted. We also have been successful in establishing an in-vitro cell line and have maintained it for over one year. The phenotype of L-8313 cells was Thy 1.2 positive. Some L-8313 cells showed a positive acid phosphatase reaction but the cytochemical character of myeloid lineage was not observed. Therefore, L-8313 is considered to be a T-cell derived hematopoietic regulatory cell neoplasm with the ability to produce several hematopoietic stimulating factors.
D000740:Anemia / Q000209:etiology; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D005260:Female; D006133:Growth Substances / Q000096:biosynthesis*; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000473:pathology; D007377:Interleukin-3 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009196:Myeloproliferative Disorders / Q000097:blood / Q000209:etiology* / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism; D009368:Neoplasm Transplantation; D010641:Phenotype; D010735:Phosphoglycerate Kinase / Q000032:analysis; D013154:Spleen / Q000473:pathology; D013163:Splenomegaly / Q000209:etiology; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D009363:Neoplasm Proteins; D018800:Thy-1 Antigens; D010735:Phosphoglycerate Kinase