Differential regulation of a Thy-1 gene in transgenic mice.
We have generated Thy-1.1-transgenic Thy-1.2 mice to study the developmental expression of the Thy-1 gene in detail by transcriptional and immunological methods. In brain, the expression of the injected gene was identical to that of the endogenous gene in a tissue- and development-specific manner. In lymphoid tissue, the transferred gene was also expressed correctly in the early phases of T-cell lineage development; however, as the T cells matured, the transcription of the transferred gene, but not the endogenous gene, was suppressed. This result shows that different regulatory elements are used to express the Thy-1 gene in early and late lymphoid development.
D000367:Age Factors; D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics*; D001921:Brain / Q000276:immunology; D003433:Crosses, Genetic; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology; D018800:Thy-1 Antigens; D014158:Transcription, Genetic
D000954:Antigens, Surface; D012333:RNA, Messenger; D018800:Thy-1 Antigens