Somatostatin inhibits insulin-stimulated amino acid uptake into cultured rat myoblasts.
The effects of somatostatin on the acute metabolic actions of insulin on newborn rat myoblasts in culture has been examined during monolayer culture. Somatostatin significantly inhibited the insulin-stimulated uptake of [3H]leucine and [3H]amino-isobutyric acid into myoblasts but had no effect on basal (unstimulated) uptake of these two substances. The lowest concentration of somatostatin to have a significant effect was 10 micrograms/l, and this was apparent in all the experiments undertaken. The inhibitory effect of somatostatin was seen at all effective concentrations of insulin used (0.3-1 U/l). These findings lend support to the concept of an endocrine role for somatostatin in vivo and suggest that a peripheral antagonism may exist between circulating insulin and somatostatin on anabolic processes such as nutrient uptake into cells.
D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D007328:Insulin / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000494:pharmacology*
D000621:Aminoisobutyric Acids; D007328:Insulin; D013004:Somatostatin; D007930:Leucine