Preparation and evaluation of anti-species and antiviral peroxidase conjugates.
Several antispecies peroxidase conjugates from human and rabbit immunoglobulins G (IgG) and a conjugate from IgG fraction of hyperimmune rabbit serum against the membranes of herpes simplex virus type 1 (HSV-1) infected cells have been prepared and characterized. The conjugates when tested in enzyme immunoassay for detection of antiherpetic antibodies in human and hyperimmune rabbit sera, as well as for detection of HSV-1 antigen in infected Vero cells appeared active and highly specific. Comparison with the peroxidase conjugates from antiherpetic IgGs prepared by means of ion- exchange and affinity chromatography has shown similar activities.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D018139:Simplexvirus / Q000276:immunology*; D014709:Vero Cells
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D006735:Horseradish Peroxidase