[Semisyngeneic transplantation of hematopoietic cells of native and cultured embryonic liver].
The protective ability and graft-versus-host (GVH) activity in parental strain hematopoietic fetal liver cells (FLC) transplanted to irradiated F1 hybrids were evaluated quantitatively. A 21-day survival of more than 80% of semi-syngeneic mouse recipients required the injection of 2-5 X 10(6) nucleated fetal liver cells (FLC). The same effect could be obtained with FLC cultivated for 4-15 days. 5 to 25 X 10(6) parental FLC were necessary to induce a considerable GVH mortality within 2-3 months after transplantation. Thus, the minimal cell doses of both native and cultivated FLC enough for the maximal protective effect have proved ineffective for the provocation of the fetal GVH disease. Hematopoietic cells from long-term FLC cultures had a low protective potential though they could contain high CFUs concentration. This discrepancy shows clearly that such polypotent precursors as CFUs have no ability to restore hematopoiesis, in other words they cannot be totipotent stem cells.
D000818:Animals; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004306:Dose-Response Relationship, Immunologic; D004622:Embryo, Mammalian; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology / Q000401:mortality; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology; D008099:Liver / Q000276:immunology; D016031:Liver Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011828:Radiation Chimera; D051381:Rats; D013997:Time Factors; D014181:Transplantation Immunology / Q000528:radiation effects; D014185:Transplantation, Isogeneic
Semisingennaia transplantatsiia krovetvornykh kletok nativnoĭ i kul'tivirovannoĭ émbrional'noĭ pecheni.