Rubredoxin as an intermediary electron carrier for nitrate reduction by NAD(P)H in Clostridium perfringens.
The NAD(P)H-dependent nitrate reductase system in Clostridium perfringens was reconstituted with rubredoxin (Rd), nitrate reductase (NaR), and an unadsorbed fraction, on a DEAE-cellulose column, of the extract (designated as fraction A), under nitrogen gas. Ferredoxin in place of Rd was not effective as an electron carrier in this reconstituted system. NAD(P)H-dependent nitrate reducing activity was also obtained by replacing fraction A with ferredoxin-NADP+ reductase from spinach. We propose the following scheme for the electron transfer in this NAD(P)H dependent nitrate reduction system. NAD(P)H----NAD(P)H-Rd reductase----Rd----NaR----NO3-.
D003016:Clostridium perfringens / Q000378:metabolism*; D004579:Electron Transport; D005288:Ferredoxins / Q000502:physiology*; D009249:NADP / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D012416:Rubredoxins / Q000502:physiology*
D005288:Ferredoxins; D009566:Nitrates; D012416:Rubredoxins; D009249:NADP