Purification of Chironex fleckeri venom components using Chironex immunoaffinity chromatography.
A comparison of the purification of the nematocyst venom of Chironex fleckeri by affinity immunochromatography using 13 different monoclonal antibodies was made. Varying degrees of purification of mouse lethal factor, hemolysin and dermonecrotic factors, as well as antigen positive proteins were achieved with each of the monoclonal antibodies. Although the protein curves of the chromatography were similar, each of the monoclonal antibody columns had a distinctive pharmacological and SDS-PAGE profile. At least two hemolysins (120,000 and 70,000 molecular weight), two dermonecrotic principles (120,000, less than 120,000) and three lethal factors (120,000, 70,000 and 14,500 molecular weight) were detected. The degree to which aggregation and fragmentation affects the molecular weights of these proteins is not known. It appears that multiple pharmacological activities are present within the same molecule since it is only with great difficulty that a pharmacological activity can be assigned to a specific molecular weight.
D000818:Animals; D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms / Q000276:immunology / Q000302:isolation & purification* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006461:Hemolysis / Q000187:drug effects; D007120:Immunochemistry; D007928:Lethal Dose 50; D008297:Male; D008387:Marine Toxins / Q000276:immunology / Q000302:isolation & purification* / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009336:Necrosis / Q000139:chemically induced
D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms; D008387:Marine Toxins