A gamma 3 hinge region probe: first specific human immunoglobulin subclass probe.
We report the first specific human immunoglobulin subclass probe which was obtained by subcloning the gamma 3 hinge region. This specific gamma 3 probe allowed us to identify with certainty the C gamma 3 gene on Southern genomic blots, to describe the first C gamma 3 restriction fragment length polymorphism (EZZ gamma 3 RF) and to show that an IgG3 selective deficiency, previously described serologically, was not due to a deletion of the C gamma 3 gene. Such a probe should be particularly useful for screening libraries from individuals with IgG3 immunodeficiencies or presenting unusual C gamma 3 genes and, consequently, for studying the C gamma gene evolution.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D052058:Hinge Exons; D006801:Humans; D007126:Immunoglobulin Allotypes; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007132:Immunoglobulin Isotypes; D007142:Immunoglobulin gamma-Chains / Q000172:deficiency / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics*; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length
D007126:Immunoglobulin Allotypes; D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes; D007142:Immunoglobulin gamma-Chains; D007136:Immunoglobulins