title: Cloning and sequence of the gene encoding the major structural component of mannose-resistant fimbriae of Serratia marcescens.
abstract: Serratia marcescens US46, a human urinary tract isolate, exhibits mannose-resistant hemagglutination and agglutinates yeast cells, thereby indicating that it has two types of adhesins. We constructed a cosmid library for the DNA of this organism and isolated DNA clones carrying genes for mannose-sensitive (MS) and mannose-resistant (MR) fimbriae. On introduction of the cloned genes into Escherichia coli K-12, MS and MR fimbriae were formed. These fimbriae were functionally and morphologically indistinguishable from those of S. marcescens. Subcloning of these gene clusters revealed that the genes encoding MS fimbriae reside on a 9-kilobase (kb) DNA fragment, while those encoding MR fimbriae are present on a 12-kb fragment. Transposon insertion and maxicell analyses revealed that formation of MR fimbriae is controlled by several genes which reside on the 9-kb fragment. The nucleotide sequence of smfA, the gene encoding the major structural component of MR fimbriae, revealed that this gene encodes a 174-amino-acid polypeptide with a typical procaryotic signal peptide. The primary structure of the smfA product showed significant homology with the primary structure of the E. coli fimbrial subunit.
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D010861:Fimbriae, Bacterial; D005798:Genes, Bacterial; D006388:Hemagglutinins / Q000235:genetics*; D008358:Mannose / Q000494:pharmacology; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D005810:Multigene Family; D010455:Peptides / Q000032:analysis / Q000235:genetics; D012706:Serratia marcescens / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006388:Hemagglutinins; D010455:Peptides; D008358:Mannose