title: Regional assignment of the erythropoietin gene to human chromosome region 7pter----q22.
abstract: The chromosomal location of the human gene for erythropoietin (EPO) was determined by Southern blot hybridization analysis of a panel of human-mouse somatic hybrid cell DNAs. DNAs from cell hybrids containing reduced numbers of human chromosomes were treated with the restriction enzyme PstI and screened with a cloned human EPO cDNA probe. EPO is assigned to human chromosome 7 based on the complete cosegregation of EPO with this chromosome in all 45 cell hybrids tested. A cell hybrid containing a translocated derivative of chromosome 7 localizes EPO to 7pter----q22. A HindIII restriction fragment length polymorphism is detected by hybridization of the EPO cDNA probe to human genomic DNA.
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004921:Erythropoietin / Q000235:genetics*; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004921:Erythropoietin; D004247:DNA; D004262:DNA Restriction Enzymes