title: Neuroendocrine gene expression in the hypothalamus: in situ hybridization histochemical studies.
abstract: 1. We have reviewed recent studies in which in situ hybridization histochemistry (ISHH) was used to investigate the regulation of expression of neurohypophysial peptides and hypothalamic releasing hormones. 2. ISHH is a technique in which the presence and quantity of a specific mRNA can be determined in tissue sections with a high degree of resolution and sensitivity. 3. ISHH has been used to measure changes in cellular levels of mRNAs encoding vasopressin, oxytocin, corticotropin-releasing factor, gonadotropin-releasing hormone, thyrotropin-releasing hormone and somatostatin in response to various physiological challenges. 4. A theme emerging from these studies is that changes in levels of mRNA encoding neuroendocrine peptides reflect changes in biosynthesis and secretion.
mesh_terms: D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D006651:Histocytochemistry; D007031:Hypothalamus / Q000378:metabolism* / Q000502:physiology; D008297:Male; D009693:Nucleic Acid Hybridization; D010906:Pituitary Hormone-Releasing Hormones / Q000378:metabolism* / Q000502:physiology; D010909:Pituitary Hormones, Posterior / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011910:Rats, Brattleboro; D013004:Somatostatin / Q000378:metabolism* / Q000502:physiology; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism / Q000502:physiology
chemical_list: D010906:Pituitary Hormone-Releasing Hormones; D010909:Pituitary Hormones, Posterior; D012333:RNA, Messenger; D013004:Somatostatin; D013973:Thyrotropin-Releasing Hormone; D003346:Corticotropin-Releasing Hormone