Studies on the energy metabolism of opossum Didelphis virginiana erythrocytes--III. Metabolic depletion with 2-deoxyglucose markedly accelerates methemoglobin reduction in opossum but not in human erythrocytes.
1. Glucose-depleted, nitrite-treated erythrocytes reduce ferriheme in vitro in an environment 100 mM to 2-deoxy-D-glucose at a rate of 2.4 microM/ml cells/hr (opossum) and 0.37 microM/ml cells/hr (human). 2. During the process of methemoglobin reduction the breakdown of adenine ribonucleotides is more rapid in opossum (0.9 microM/g hg/hr) than in human (0.36 microM/g hg/hr) erythrocytes. 3. Radiolabelled ribose from [U-14C] ATP is catabolized exclusively to [14C] lactate in opossum, and to [14C] pyruvate and [14C] lactate in human red cells.
D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D003837:Deoxy Sugars / Q000097:blood*; D003847:Deoxyglucose / Q000097:blood*; D004734:Energy Metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008706:Methemoglobin / Q000378:metabolism*; D009243:NAD / Q000097:blood; D009893:Opossums / Q000097:blood*; D010084:Oxidation-Reduction; D013045:Species Specificity
D003837:Deoxy Sugars; D009243:NAD; D000255:Adenosine Triphosphate; D008706:Methemoglobin; D003847:Deoxyglucose