3-Methylcholanthrene does induce mixed function oxidase activity in hepatopancreas of spiny crab Maja crispata.
1. Type II inducers (7,8-benzoflavone, benzo(a)-pyrene and 3-methylcholanthrene) as well as Aroclor 1254, significantly increase benzo(a)pyrene monooxygenase activity in crab hepatopancreas while type I inducer (phenobarbital) does not enhance benzo(a)pyrene monooxygenase activity. 2. 3-methylcholanthrene and benzo(a)pyrene treatment of crabs significantly increase cytochrome P-450 content. 3. Benzo(a)pyrene monooxygenase induction in hepatopancreas of 3-methylcholanthrene treated crabs was inhibited by simultaneous treatment with cycloheximide but not by actinomycin D. 4. Actinomycin D insensitivity can be explained involving a regulatory pattern of induction on the posttranscriptional and/or translational, rather than transcriptional level.
D000818:Animals; D003386:Brachyura / Q000201:enzymology*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction; D008099:Liver / Q000201:enzymology; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D010179:Pancreas / Q000201:enzymology; D014176:Protein Biosynthesis / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects
D003609:Dactinomycin; D008748:Methylcholanthrene; D003513:Cycloheximide; D006899:Mixed Function Oxygenases