Polymorphic Bgl II restriction sites of DR alpha demarcate a novel HLA-DR1 antigen.
Mechanisms to account for the unusual properties of a DR1 alpha beta complex (designated DRgp50) that is resistant to dissociation under normal conditions utilized were investigated. Expression of this DRgp50 complex is highly correlated with the failure of cells from certain DR1 individuals (DR1x) to stimulate specific DR1-restricted or alloreactive T-cell clones. Pulse/chase experiments demonstrated that this DRgp50 complex was not detectable until approximately 1 h of chase. The DR1 alpha and beta chains associated into the heterodimer in the absence of glycosylation and alterations in the number of oligosaccharides or sialylation of cell surface forms were not evident when compared with normal DR1 alpha and beta chains. Restriction fragment length polymorphism patterns of DR beta genes from normal (DR1n) and DR1x individuals were indistinguishable. However, a difference in the alpha chain genes between DR1n and DR1x individuals was revealed using Bgl II. This Bgl II restriction site mapped to the 3' untranslated region of DR alpha and represents a new genomic marker to distinguish this functional and biochemical variant of DR1.
D001426:Bacterial Proteins; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D005802:Genes, MHC Class II; D006026:Glycoside Hydrolases / Q000378:metabolism; D006681:HLA-D Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006239:Haplotypes; D006358:Hot Temperature; D006801:Humans; D046911:Macromolecular Substances; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D008970:Molecular Weight; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011499:Protein Processing, Post-Translational; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D012794:Sialic Acids / Q000502:physiology; D014415:Tunicamycin / Q000494:pharmacology
D001426:Bacterial Proteins; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D011948:Receptors, Antigen, T-Cell; D012794:Sialic Acids; D014415:Tunicamycin; D004262:DNA Restriction Enzymes; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific; D006026:Glycoside Hydrolases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase