Free fatty acid uptake is increased in doxorubicin-resistant rat glioblastoma cells.
We have investigated the mechanism by which the rat glioblastoma C6 cell line resistant to doxorubicin presented an increase of free fatty acid utilization from the nutrient source as compared to the wild sensitive strain. We have shown that this was not due to an accelerated turnover of the phospholipid hydrophobic or hydrophilic moieties, but to an important increase of the uptake of the free fatty acids. This was demonstrated by studies performed with linoleic and linolenic acids during very short incubations, at low temperatures, and in the absence of albumin in the medium. This enhancement of free fatty acid uptake may explain the differences which exist in the acyl group composition of membrane lipids between doxorubicin-sensitive and -resistant C6 cells, because of the suppression of the essential fatty acid-deficient status of the cells.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism*; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017962:alpha-Linolenic Acid
D000085:Acetates; D005230:Fatty Acids, Nonesterified; D008041:Linoleic Acids; D008042:Linolenic Acids; D010710:Phosphates; D010743:Phospholipids; D017962:alpha-Linolenic Acid; D004317:Doxorubicin; D019787:Linoleic Acid; D019342:Acetic Acid