Inhibition of trout (Salmo gairdneri R.) PBG-synthase by some metal ions (Mg2+, Pb2+, Zn2+).
The effect of metal ions on the activity of trout kidney and liver PBG-synthase was investigated. Heavy metals inhibited the kidney enzyme in a complex manner. Kinetic analysis of the inhibition of liver activity by Pb2+ (Ki = 1.3 mM) was consistent with non-competitive inhibition, whereas Zn2+ (Ki = 1.3 mM) and Mg2+ (Ki = 3.5 mM) were competitive inhibitors.
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D007854:Lead / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors*; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D008670:Metals; D007854:Lead; D000623:Porphobilinogen Synthase; D008274:Magnesium; D015032:Zinc