title: Localization of dynorphin gene product-immunoreactivity in neurons from spinal cord and dorsal root ganglia.
abstract: Using spinal cord and dorsal root ganglion cell cultures, we have studied the immuno-histochemical distribution of several peptide products of the dynorphin gene. With antibody directed toward the midregion of dynorphin A, peptide-immunoreactivity was found exclusively in the cell bodies of spinal cord neurons. Antibody directed toward the amino- or carboxy-terminus of dynorphin A revealed peptide-immunoreactivity in the neurites, as well as perikarya. Spinal cord neurons also expressed dynorphin B- and alpha-neo-endorphin-immunoreactivities in both cell bodies and neurites. Dorsal root ganglion neurons cultured from embryonic tissue expressed dynorphin A-(1-13)-, dynorphin A-(9-17)- and dynorphin B-immunoreactivities in their perikarya. Sensory neurons obtained from dissociated adult ganglia similarly expressed dynorphin-immunoreactivity immediately upon inoculation into culture. Embryonic and adult murine sensory ganglia from the sacral region more frequently expressed dynorphin than did cells obtained from other spinal levels. Expression of dynorphin-immunoreactivity by sensory neurons was not influenced by elevated levels of Nerve Growth Factor or spinal cord conditioned medium. These data indicate that intrinsic spinal cord neurons may modulate sensory and spinal function in rather subtle ways via the expression of several different opioid peptide products of the dynorphin gene, in addition to the opioid peptides produced by the proenkephalin A gene. Beyond this, the observation of dynorphin-related peptides in dorsal root ganglion neurons suggests that these opioid peptides may have a specialized role in primary afferent neurotransmission.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002478:Cells, Cultured; D004399:Dynorphins / Q000031:analogs & derivatives / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D004723:Endorphins / Q000032:analysis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000032:analysis / Q000166:cytology*; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D013116:Spinal Cord / Q000032:analysis / Q000166:cytology*
chemical_list: D000906:Antibodies; D004723:Endorphins; D004399:Dynorphins; C036200:rimorphin