Polyadenylylation of an mRNA precursor occurs independently of transcription by RNA polymerase II in vivo.
Most eukaryotic messenger RNAs are transcribed as precursor molecules that must be processed by capping, splicing, 3' cleavage, and polyadenylylation to yield mature mRNAs. An important, unresolved issue is whether any of these reactions are linked either to transcription by RNA polymerase II or to each other. To address one aspect of this question, we constructed a chimeric gene containing an RNA polymerase III promoter (the adenovirus VAI promoter) fused to the body and 3'-flanking sequences of a protein-coding gene (the herpesvirus tk gene). Here we show that this hybrid gene was transcribed from the RNA polymerase III promoter following transfection of human 293 cells and that the transcripts produced were stable and efficiently transported to the cytoplasm. Although a significant proportion of the transcripts were prematurely terminated at specific sites within the gene, a high percentage of the full-length RNA was accurately cleaved and polyadenylylated. These results demonstrate that cleavage and polyadenylylation of mRNA precursors are not obligatorily coupled to transcription by RNA polymerase II in vivo.
D004720:Endonucleases / Q000494:pharmacology; D006801:Humans; D009694:Nucleic Acid Precursors / Q000378:metabolism*; D009710:Nucleotide Mapping; D011061:Poly A / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000494:pharmacology*; D012320:RNA Polymerase III / Q000494:pharmacology; D012322:RNA Precursors; D012333:RNA, Messenger / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic
D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; D011061:Poly A; D013937:Thymidine Kinase; D012319:RNA Polymerase II; D012320:RNA Polymerase III; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases