Bradykinin activates tyrosine hydroxylase in rat pheochromocytoma PC-12 cells.
Tyrosine hydroxylase is activated in PC-12 cells by bradykinin in a concentration-dependent manner with maximal stimulation occurring at 1 microM. This stimulatory effect occurs within 15 s and is maximal at 5 min. This stimulation is due to an increase in the affinity of tyrosine hydroxylase for its pterin cofactor, and can be blocked by a specific bradykinin receptor antagonist. These data indicate that bradykinin can regulate the activity of tyrosine hydroxylase in PC-12 cells.
D000310:Adrenal Gland Neoplasms / Q000201:enzymology*; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D010673:Pheochromocytoma / Q000201:enzymology*; D051381:Rats; D014407:Tumor Cells, Cultured; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase; D001920:Bradykinin