Cloning, sequencing and analysis of the yeast S. uvarum ERG10 gene encoding acetoacetyl CoA thiolase.
The ERG10 gene specific to S. uvarum, a brewing yeast, has been cloned by complementation of an S. cerevisiae erg10 mutant. S. uvarum contains two different ERG10 genes. One of these is similar to the S. cerevisiae ERG10 gene; they are structurally different, but functionally homologous. The cloned ERG10 gene has been located on chromosome XVI, and we have shown that it is allelic to the previously isolated tsm0115 mutants. Northern blot and sequence analysis indicate that the ERG10 gene is highly expressed, and biochemical and genetic evidence show that it encodes the cytoplasmic acetoacetyl CoA thiolase.
D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics*; D000123:Acetyltransferases / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D002875:Chromosomes; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006358:Hot Temperature; D007158:Immunologic Techniques; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012331:RNA, Fungal / Q000302:isolation & purification; D012440:Saccharomyces / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic
D004271:DNA, Fungal; D007527:Isoenzymes; D012331:RNA, Fungal; D000123:Acetyltransferases; D000101:Acetyl-CoA C-Acetyltransferase