mec-3, a homeobox-containing gene that specifies differentiation of the touch receptor neurons in C. elegans.
The mec-3 gene is essential for proper differentiation of the set of six touch receptor neurons in C. elegans. In mutants lacking mec-3 activity, the touch receptors express none of their unique differentiated features and appear to be transformed into other types of neurons. We cloned the mec-3 gene by transposon tagging and showed that a mec-3 mutant can be rescued by germ line transformation using a 5.6 kb genomic DNA fragment. In a strain in which transforming mec-3 DNA is present in about 50 copies per haploid genome, additional cells express a mec-3-dependent phenotype. The putative coding sequence of mec-3 contains a homeobox, suggesting that the mec-3 protein specifies the expression of touch cell differentiation by binding to DNA and regulating transcription of genes that encode the differentiated features of these cells.
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002107:Caenorhabditis / Q000235:genetics*; D002454:Cell Differentiation; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005091:Exons; D005801:Genes, Homeobox; D007438:Introns; D008465:Mechanoreceptors / Q000166:cytology*; D008969:Molecular Sequence Data; D009154:Mutation; D009475:Neurons, Afferent / Q000166:cytology*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D014170:Transformation, Genetic
D004247:DNA