title: Isolation of a cDNA for the rat heavy neurofilament polypeptide (NF-H).
abstract: We have isolated from a rat brain lambda gt11 expression library two overlapping cDNA clones of sizes 2.5 and 3.0 kb corresponding to the heavy neurofilament polypeptide (NF-H). The 2.5 kb insert apparently represents virtually the whole of the C-terminal tail, the 3.0 kb insert also encodes the conserved epitope for the monoclonal antibody, anti-IFA. The identity of the cDNAs was established by comparison of the predicted amino acid sequence with the known partial amino acid sequence of porcine NF-H. A repeat peptide sequence that may be a multiphosphorylation site was identified in the C-terminal non-helical tail.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D007381:Intermediate Filament Proteins / Q000235:genetics*; D016900:Neurofilament Proteins; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
chemical_list: D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA