A direct evidence for the involvement of poly(A) in protein synthesis.
A radioactive polyadenylated globin mRNA was translated in either rabbit reticulocyte lysate or wheat germ extract under various conditions. When globin mRNA was translated, globin synthesis was directly proportional to the rate of loss in A units from the poly(A) tail. On the other hand, when globin poly(A) mRNA was incubated under non-translated conditions, no loss of A units was detected. The presence of ribonuclease inhibitor in the reaction mixture did not alter either the rate of globin synthesis or the loss in A units from the poly(A) tail. The present data suggests a correlation between protein synthesis and loss in A units from the poly(A) tail.
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D005914:Globins / Q000235:genetics*; D007700:Kinetics; D010944:Plants / Q000378:metabolism; D011061:Poly A / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D013816:Thermodynamics; D014908:Triticum / Q000378:metabolism
D012333:RNA, Messenger; D011061:Poly A; D005914:Globins; D003513:Cycloheximide