Beta-endorphin modification by transglutaminase in vitro: identification by FAB/MS of glutamine-11 and lysine-29 as acyl donor and acceptor sites.
FAB-Mapping strategy was successfully exploited to characterize the reaction products of the transglutaminase-mediated modifications of human beta-endorphin in vitro. The GLN-11 residue of the neuropeptide was shown to be an effective acyl donor site for the enzyme, being able to bind spermine in the presence of Ca2+. Moreover, only one out of five lysyl residues (LYS-29) was demonstrated to act as acyl acceptor crosslinking with GLN-11.
D000215:Acylation; D000595:Amino Acid Sequence; D005973:Glutamine / Q000032:analysis*; D006801:Humans; D008239:Lysine / Q000032:analysis*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011503:Transglutaminases / Q000378:metabolism*; D001615:beta-Endorphin / Q000494:pharmacology*
D005973:Glutamine; D001615:beta-Endorphin; D011503:Transglutaminases; D008239:Lysine