Effect of high doses of somatostatin on adenylate cyclase activity in peripheral mononuclear leukocytes from normal subjects and from acute leukemia patients.
In normal lymphocytes somatostatin non-competitively inhibited basal (ID50 5 x 10(-4) M) and isoproterenol- and forskolin-stimulated adenylate cyclase activity (Ac). In acute leukemia blasts, non-responsive to isoproterenol, forskolin, which activates the catalytic subunit, stimulated and somatostatin inhibited Ac, thus indicating the leukemic enzyme, though defective, retains the inhibitory pathway and catalyst function.
D000208:Acute Disease; D000262:Adenylyl Cyclases / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D007938:Leukemia / Q000201:enzymology*; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D013004:Somatostatin / Q000008:administration & dosage*
D005576:Colforsin; D013004:Somatostatin; D000262:Adenylyl Cyclases; D007545:Isoproterenol