[Compartmentalization of Ca2+ in subcellular structures of the rat liver in chemical injury of hepatocyte membranes].
Compartmentalization of Ca2+ was studied in homogenates and subcellular fractions (nuclei, mitochondria, microsomes and cytosol fraction) of rat liver tissue under conditions of chemical lesion of hepatocyte membranes caused by CCl4. The studies were carried out within 2, 24, 48, 96 and 168 hrs after the CCl4 administration. The chemical lesion of hepatocyte membranes led to considerable increase in Ca2+ concentration in the subcellular fractions studied, especially in cytosol fraction, where it reached about 424% as compared with the control level. The drastic increase of calcium concentration in cytosol appears to be due to disturbance of both Ca2+ elimination through plasmatic membrane and its mitochondrial sequestration.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism; D008933:Mitochondrial Swelling; D006180:Proton-Translocating ATPases / Q000378:metabolism; D051381:Rats
D004533:Egtazic Acid; D002251:Carbon Tetrachloride; D006180:Proton-Translocating ATPases; D002118:Calcium
Kompartmentalizatsiia Ca2+ v subkletochnykh strukturakh pecheni pri khimicheskom povrezhdenii membran gepatotsitov.