Diagnosis of renal allograft rejection by analysis of fine-needle aspiration biopsy specimens with immunostains and simple cytology.
Fine-needle aspiration biopsy specimens of renal transplants were analysed by means of commercially available monoclonal antibodies and an immunoperoxidase stain. Three cellular features associated with acute cellular rejection were identified--heavy infiltrates of activated T cells or large mononuclear cells strongly expressing HLA-DR antigens, and HLA-DR expression by renal tubular cells. A combination of semiquantitative scores for these features correctly identified rejection in 32 of 34 cases, with no false positives in cases of cyclosporin nephrotoxicity or stable graft function.
D000911:Antibodies, Monoclonal; D001379:Azathioprine / Q000627:therapeutic use; D001707:Biopsy, Needle; D003404:Creatinine / Q000097:blood; D003524:Cyclosporins / Q000627:therapeutic use; D006084:Graft Rejection; D006684:HLA-DR Antigens / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D016030:Kidney Transplantation; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D003524:Cyclosporins; D006684:HLA-DR Antigens; D003404:Creatinine; D001379:Azathioprine