Immunochemical characterisation of somatostatin in ruminants.
Following development and validation of a radioimmunoassay for somatostatin, the immunoreactivity of this peptide in the plasma of ruminants was measured and the levels in sheep were 9-31 pM (mean 18 +/- 7 pM, n = 48), in lambs 10-54 pM (mean 25 +/- 10 pM, n = 18) and in calves 5-35 pM (mean 12 +/- 6 pM, n = 22). Somatostatin-like immunoreactivity was present in sheep in high concentrations in the antrum (2342 +/- 280 pmol/g wet weight), duodenum (446 +/- 73 pmol/g) and pancreas (832 +/- 208 pmol/g). Lower concentrations (6-150 pmol/g) were found in other regions of the gastrointestinal tract. Molecular sieve chromatography on Bio-Gel P-10 showed that while most of the somatostatin in the antrum was somatostatin-14, in the duodenum about 30% of the total immunoreactivity was somatostatin-28.
D000818:Animals; D002417:Cattle; D004386:Duodenum / Q000378:metabolism; D007120:Immunochemistry; D010179:Pancreas / Q000378:metabolism; D010455:Peptides / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D011706:Pyloric Antrum / Q000378:metabolism; D011863:Radioimmunoassay; D012756:Sheep; D013004:Somatostatin / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D014018:Tissue Distribution
D010455:Peptides; C029993:somatostatin-like peptides; D013004:Somatostatin