title: An improved method for adrenalectomy of suckling rats. The influence of thrombin treatment and deoxycorticosterone substitution on survival and on hepatic and renal enzyme activities.
abstract: Adrenalectomy of suckling rats is complicated by a high mortality rate, caused by the loss of blood (early mortality) and by the disturbed sodium-potassium balance (late mortality). Treatment of the abdominal cavity with a thrombin solution and a daily administration of deoxycorticosterone glucoside (DOC) decrease the total mortality remarkably. DOC treatment has no influence on renal beta-glucosidase and beta-galactosidase as well as on hepatic tyrosine aminotransferase activity, whereas hepatic serine dehydratase activity exhibits a time- and dosage-dependent response to this hormone. The DOC effect is very likely a consequence of the glucocorticoid-like action of the synthetic hormone, which competes with the endogenous glucocorticoids for the hepatic receptor molecules.
mesh_terms: D000311:Adrenal Glands / Q000254:growth & development; D000315:Adrenalectomy / Q000379:methods*; D000375:Aging; D000818:Animals; D003900:Desoxycorticosterone / Q000494:pharmacology*; D005696:Galactosidases / Q000378:metabolism*; D005938:Glucocorticoids / Q000097:blood; D005959:Glucosidases / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D012695:L-Serine Dehydratase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013917:Thrombin / Q000494:pharmacology*; D014444:Tyrosine Transaminase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D005938:Glucocorticoids; D003900:Desoxycorticosterone; D014444:Tyrosine Transaminase; D005696:Galactosidases; D005959:Glucosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D013917:Thrombin; D012695:L-Serine Dehydratase