title: Solid-phase extraction and ion-pair reversed-phase HPLC of isometamidium in bovine serum and tissues.
abstract: An analytical method has been developed for the determination of isometamidium in bovine serum and tissues. Samples were enzymatically hydrolysed and cleaned up on a solid-phase system (C8 Bond Elut column). The drug was chromatographed by an ion-pair reversed-phase technique using heptane sulphonate as a pairing-ion and triethylamine as a counter-ion reagent. Detection was by fluorescence at 593 nm (excitation = 380 nm). The method is more sensitive and specific than existing methods and it is currently being used in evaluating the pharmacokinetics of isometamidium in cattle.
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D009132:Muscles / Q000032:analysis; D010617:Phenanthridines / Q000032:analysis* / Q000097:blood
chemical_list: D010617:Phenanthridines; C000702:isometamidium chloride