Localisation of the hydrophilic C terminal part of the ATP synthase subunit 8 of Saccharomyces cerevisiae.
The hydrophobic subunit 8 of the yeast ATP synthase was modified using the non-penetrating amino reactive specific reagent: isethionylacetimidate. The polypeptide was modified when using the isolated ATP synthase and sodium bromide-treated submitochondrial particles. It is shown that the only lysine of the protein was modified by the reagent. It is concluded that the hydrophilic C terminal part of the protein containing lysine 47 is located on the inner side of the inner mitochondrial membrane.
D000595:Amino Acid Sequence; D001965:Bromides / Q000494:pharmacology; D007096:Imidoesters / Q000494:pharmacology; D007425:Intracellular Membranes / Q000201:enzymology*; D007513:Isethionic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000032:analysis; D011510:Proteolipids / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012964:Sodium / Q000494:pharmacology; D017670:Sodium Compounds; D013367:Submitochondrial Particles / Q000201:enzymology
D001965:Bromides; D007096:Imidoesters; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011510:Proteolipids; D017670:Sodium Compounds; C020761:isethionyl acetimidate; D007513:Isethionic Acid; D012964:Sodium; D006180:Proton-Translocating ATPases; C027938:sodium bromide