Expression of the S-1 catalytic subunit of pertussis toxin in Escherichia coli.
The S-1 subunit of pertussis toxin was expressed as a fusion protein in a strain of Escherichia coli deficient in protein degradation. The fusion protein reacted with anti-pertussis toxin antibody, and, like authentic pertussis toxin, it ADP-ribosylated a 41,000-molecular-weight membrane protein from human erythrocytes.
D001886:Bordetella pertussis / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D037342:Pertussis Toxin; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D010566:Virulence Factors, Bordetella / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin