Identification of HIV-infected seronegative individuals by a direct diagnostic test based on hybridisation to amplified viral DNA.
There is a need for direct detection of the virus in people infected with human immunodeficiency virus (HIV), independently of a serological response. In this study, after enzymic amplification of a specific segment of the HIV genome, a simple slot-blot hybridisation procedure allowed unequivocal identification of HIV DNA in all seropositive subjects tested. More importantly, the hybridisation test allowed the detection of HIV DNA in several seronegative subjects from very high risk groups. This new direct approach towards the diagnosis of HIV infection, which can easily be carried out on a large scale, is therefore capable of identifying HIV-infected individuals before the development of antibodies.
D004279:DNA, Viral / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D005814:Genes, Viral; D006678:HIV / Q000235:genetics / Q000302:isolation & purification*; D006679:HIV Seropositivity / Q000175:diagnosis*; D006801:Humans; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase