Monoclonal antibodies neutralizing the antiviral and the antiproliferative activities of human interferon gamma.
Spleen cells from BALB/c mice immunized with human leukocyte interferon gamma (HuIFN-gamma) were fused with mouse NSO myeloma cells. Nine hybridoma lines were found secreting monoclonal antibodies (MoAb) which were able to neutralize the antiviral activity of HuIFN-gamma. All nine MoAb inhibited also the antiproliferative activity of HuIFN-gamma. The ability of tested MoAb to inhibit both the antiviral and antiproliferative activities of HuIFN-gamma supports the suggestion that a common domain on HuIFN-gamma molecule might be responsible for both biological activities. However, ELISA and/or RIA proved unsuitable for detection of these specific MoAb.
D000818:Animals; D000911:Antibodies, Monoclonal; D000998:Antiviral Agents / Q000037:antagonists & inhibitors; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D007371:Interferon-gamma