Use of Chinese hamster ovary cells with altered glycosylation patterns to define the carbohydrate specificity of Entamoeba histolytica adhesion.
We compared the adherence of E. histolytica trophozoites with a panel of lectin-resistant CHO mutants with altered glycosylation patterns. Our results coupled with data from saccharide inhibition studies indicate that terminal N-acetyllactosamine units on Asn-linked complex type oligosaccharides provide the major determinants on the cellular receptor for E. histolytica adhesion.
D000818:Animals; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance; D004748:Entamoeba histolytica / Q000502:physiology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006031:Glycosylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000502:physiology; D010053:Ovary / Q000166:cytology; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
D009844:Oligosaccharides; D014909:Wheat Germ Agglutinins