title: Development of adult T-cell leukemia-like disease in African green monkey associated with clonal integration of simian T-cell leukemia virus type I.
abstract: Proviral integration of a simian retrovirus highly homologous to human T-cell leukemia virus type I was examined in cellular DNAs extracted from primary peripheral blood lymphocytes of 31 adult African green monkeys (Cercopithecus aethiops) that were seropositive for simian T-cell leukemia virus type I (STLV-I). Among these monkeys, one case with overt leukemia, showing pleomorphic leukemia cells similar to those in human adult T-cell leukemia (ATL), and five cases in a preleukemic state of ATL-like disease were found. Judging from the integration site of the provirus genome, primary lymphocytes of these leukemic or preleukemic cases contained monoclonally proliferated STLV-I-infected cells, whereas lymphocytes of other seropositive monkeys without hematological abnormalities were polyclonal, and those of seronegative monkeys did not contain the provirus. The restriction patterns with PstI ans SstI of most STLV-I proviruses were identical to those of the previous isolate from this species, but in three monkeys there was a deletion of one PstI site. From the correlation of the development of simian ATL-like disease with the monoclonal integration of the STLV-I provirus genome, it should be indicated that STLV-I has similar leukemogenicity to human T-cell leukemia virus type I, and so STLV-I infection in African green monkeys will be useful as an animal model of human ATL.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002521:Cercopithecus; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000032:analysis; D017977:Deltaretrovirus / Q000235:genetics; D006800:Deltaretrovirus Infections / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D005814:Genes, Viral; D008214:Lymphocytes / Q000382:microbiology; D008992:Monkey Diseases / Q000097:blood / Q000209:etiology*; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral