Growth curves of Clostridium perfringens in Schaedler and brain heart infusion broths.
Four Clostridium perfringens strains recovered from clinical specimens, four strains obtained from faecal specimens of healthy adults, and control strain ATCC 13124 were inoculated into Schaedler and Brain Heart Infusion broths, respectively, and incubated anaerobically at 35 degrees C for 24 h. The exponential growth phase usually lasted from 2 to 6 h following inoculation; the generation times averaged 23 min at 2 to 4 h and 27 min between 4 and 6 h of incubation. The early stationary growth phase (generation time between 6 and 8 h = 89 min) was reached at 8 h following inoculation. All strains displayed essentially identical in vitro growth rates, even though they differed in murine virulence.
D000818:Animals; D003016:Clostridium perfringens / Q000254:growth & development* / Q000472:pathogenicity; D003470:Culture Media; D006801:Humans; D051379:Mice; D014774:Virulence
D003470:Culture Media