[Protective potential of hematopoietic cells of the embryonic liver during long-term culture].
The influence of the duration of the organ cultivation on the protective potential of mouse fetal liver hemopoietic cells was investigated. The protective potential was evaluated according to a 3-week viability of the lethally irradiated (mean LD88/21) mice following syngeneic transplantation. During 20 days of cultivation the protective potential (calculated per number of injected cells) remained at the initial level. With more prolonged cultivation (24-62 days) the protective potential was retained only in part of cultures and the mean effect was reduced. No parallelism has been revealed between the totipotent hemopoietic precursors capable of replacing the hemopoietic cells of an irradiated recipient and the CFUs in long-cultivated fetal liver cultures.
D000818:Animals; D004622:Embryo, Mammalian; D005260:Female; D005720:Gamma Rays; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000528:radiation effects; D007621:Karyotyping; D008099:Liver / Q000166:cytology* / Q000528:radiation effects; D016031:Liver Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009924:Organ Culture Techniques; D013997:Time Factors; D014185:Transplantation, Isogeneic
Zashchitnyĭ potentsial krovetvornykh kletok émbrional'noĭ pecheni v protsesse dlitel'nogo kul'tivirovaniia.