Isolation and characterization of brush border membrane vesicles from pig small intestine.
1. Brush border membrane vesicles (BBMV) were isolated from swine mid-intestine by a MgCl2 precipitation and sucrose density gradient centrifugation. 2. Transport of D-glucose and L-alanine were Na+-stimulated and into an osmotically sensitive space. 3. Estimates of kinetic parameters for Na+-dependent D-glucose transport were: apparent Kt = 1.8 mM and Jmax = 16.8 nmol/mg protein/min. 4. Results of experiments with the delta pH sensitive fluorescent probe 9-aminoacridine indicated independent mechanisms for Na+-dependent glucose transport and Na+/H+ exchange. 5. This study demonstrates that pig BBMV provide a useful model for investigating intestinal membrane transport.
D000409:Alanine / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient / Q000379:methods; D005947:Glucose / Q000378:metabolism; D007421:Intestine, Small / Q000648:ultrastructure*; D007700:Kinetics; D008564:Membrane Potentials; D008871:Microvilli / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D013552:Swine
D005947:Glucose; D000409:Alanine