Experimental inoculation of monkeys with autologous lymphoid cell lines immortalized by and producing human T-cell leukemia virus type-I.
Cynomolgus monkeys and squirrel monkeys were inoculated with autologous lymphoid cell lines immortalized by and producing human T-cell leukemia virus type-I (HTLV-I) in order to serve as an animal model of adult T-cell leukemia (ATL). The autologous cell lines were established from peripheral blood mononuclear cells (PBMC) from each monkey by co-cultivation with lethally irradiated MT-2 cells producing HTLV-I. All of these cell lines, which had monkey karyotypes, grew continuously without addition of interleukin-2 (IL-2) and expressed virus-specific proteins of HTLV-I and IL-2 receptor. After inoculation with the autologous cell lines, specific antibodies against HTLV-I proteins could be detected in their plasma, and transformed HTLV-I-infected cells could be recovered from their peripheral blood for at least 6 months. However, no signs of ATL have been observed to data, i.e. 2 years after inoculation.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D002460:Cell Line; D002472:Cell Transformation, Viral; D006800:Deltaretrovirus Infections / Q000276:immunology*; D015488:HIV Antigens; D008214:Lymphocytes / Q000382:microbiology*; D008252:Macaca fascicularis; D008297:Male; D011971:Receptors, Immunologic / Q000032:analysis; D015375:Receptors, Interleukin-2; D012453:Saimiri
D000956:Antigens, Viral; D015488:HIV Antigens; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2