Intracellular distribution of mammalian stress proteins. Effects of cytoskeletal-specific agents.
Following a brief period of heat stress, the two highly conserved mammalian stress proteins, hsp68 and 70, were examined with respect to their intracellular locations. In four independent cell lines, hsp68 and 70 were found to partition into both Triton X-100-soluble and insoluble fractions as assessed by two-dimensional gel analysis of newly synthesized polypeptides, whereas a fifth cell line showed these proteins only in the Triton X-100-insoluble fraction. In addition, a previously described cell fractionation technique was utilized to gain information regarding the segregation of the two major mammalian stress proteins, hsp68 and 70, into distinct biochemically and morphologically characterized subcellular compartments of PtK2-epithelial cells. Two cytoskeletal-specific agents, taxol and colchicine, were also probed for their effects on the disposition of these polypeptides. Under our conditions of acute heat exposure, hsp68, 70 and their isoforms were globally distributed in all subcellular fractions examined, with a few notable exceptions in drug-treated cells. Colchicine, a microtubule-depolymerizing drug, inhibited the association of hsp68 and its variants with the double-detergent-extractable labile "cytoskeleton," whereas taxol, a microtubule-stabilizing agent, in some manner, facilitated the transit of hsp68 and its isovariants from a cytoplasmic to nuclear domain. Degree of cell density is a factor which influences the synthesis of various cytoskeletal proteins; therefore, we studied the effect of cell confluency on the disposition of mammalian stress proteins hsp68 and 70 in human FS-4 fibroblasts. In confluent cultures, where cell-cell contact was maximal, we observed the appearance of a previously undetected polypeptide which was not found in sparsely populated cultures. This protein may represent a post-translationally modified isoform of a preexisting heat shock protein, or perhaps, a novel stress protein.
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002452:Cell Count; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D005470:Fluorometry; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008322:Mammals; D017830:Octoxynol; D017239:Paclitaxel; D011092:Polyethylene Glycols; D012995:Solubility; D014018:Tissue Distribution
D000470:Alkaloids; D006360:Heat-Shock Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; D017239:Paclitaxel; D003078:Colchicine