New sensitive high-performance liquid chromatographic method for p-tyrosine aminotransferase assay.
A rapid, sensitive and specific procedure has been developed for the determination of p-tyrosine aminotransaminase activity. The assay is based on high-performance liquid chromatography (HPLC) separation and electrochemical detection of the pyruvate product, which has been derivatized with hydroxylamine to form a stable oxime. Using this method the product at the low pmol level can be measured. A comparison of the kinetic parameters of the rat liver tyrosine aminotransferase and rat brain non-specific aspartate aminotransferase towards p-tyrosine has been made.
D000818:Animals; D001921:Brain / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D010667:Phenylpyruvic Acids / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D014444:Tyrosine Transaminase / Q000032:analysis*
D010667:Phenylpyruvic Acids; C010590:4-hydroxyphenylpyruvic acid; D014444:Tyrosine Transaminase