A technique for simultaneous microangiographic and cytochemical investigation of resin-embedded tissues.
A method for simultaneous acquisition of information on cytochemical and microangiographic characteristics of tissue after perfusion with contrast medium was developed. The technique is based on the use of hydrosoluble resin as embedding material and is illustrated by the demonstration of gamma-glutamyltranspeptidase activity and of the microcirculation in rat liver during chemically induced hepatocarcinogenesis and experimental metastasis. The method is applicable to investigations aimed at correlating microangiographic and cytochemical characteristics of normal or altered tissues.
D000792:Angiography / Q000379:methods*; D000818:Animals; D003287:Contrast Media; D006651:Histocytochemistry / Q000379:methods*; D008099:Liver / Q000000981:diagnostic imaging / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000201:enzymology; D008833:Microcirculation / Q000033:anatomy & histology; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012116:Resins, Plant; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D003287:Contrast Media; D012116:Resins, Plant; D005723:gamma-Glutamyltransferase