title: Changes in the secretion of ABP into testicular interstitial fluid with age and in situations of impaired spermatogenesis.
abstract: Androgen-binding protein (ABP) was measured in serum and testicular interstitial fluid (IF) from rats during sexual maturation or in adult rats in which impairment of spermatogenesis had been induced by (i) testosterone withdrawal following Leydig cell destruction, (ii) local heating (43 degrees C) of the testes for 30 min or (iii) induction of unilateral cryptorchidism (UCD). The changes observed were related to the IF levels of testosterone and, in most instances, to the serum levels of FSH. The levels of ABP in serum and IF decreased together with age, being highest at 30 days, falling steeply by 40 days and then slowly but progressively up to 100 days of age. A similar pattern was observed for serum FSH, except that the initial fall occurred beyond 40 days of age. Treatment with EDS or exposure to local heating caused comparable reductions in testicular weight (25-30% by 7 days after treatment, 50% by 21-28 days) and raised the serum levels of FSH. In both groups the levels of ABP in IF were increased by two- to three-fold while the levels of testosterone were either reduced markedly (EDS-treatment) or remained unchanged (local heating). In rats made UCD for 60 days, the weight of the abdominal testis was reduced by 75%, compared with the contralateral scrotal testis, while the IF levels of ABP and testosterone were significantly increased (55%) and decreased (90%), respectively. Short-term (3 days) deprivation of testosterone in adult rats, following immunoneutralization of LH, was without significant effect on IF levels of ABP. It is concluded that ABP secretion into IF is increased in situations of subnormal (or sub-adult) numbers of germ cells and this is usually associated with high levels of FSH. Measurement of ABP levels in IF should prove of value for the monitoring of Sertoli cell function in vivo and may be of diagnostic use for the detection of changes in germ cell numbers.
mesh_terms: D000375:Aging / Q000097:blood / Q000378:metabolism*; D000727:Androgen-Binding Protein / Q000097:blood / Q000378:metabolism*; D000818:Animals; D003456:Cryptorchidism / Q000097:blood; D005640:Follicle Stimulating Hormone / Q000097:blood; D006358:Hot Temperature; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D008698:Mesylates; D051381:Rats; D011919:Rats, Inbred Strains; D013091:Spermatogenesis / Q000187:drug effects; D013739:Testosterone / Q000097:blood / Q000378:metabolism
chemical_list: D000727:Androgen-Binding Protein; D008698:Mesylates; D013739:Testosterone; D005640:Follicle Stimulating Hormone; C002994:ethylene dimethanesulfonate