The use of gold-substituted silver-intensified diaminobenzidine (DAB) and non-intensified DAB for simultaneous electron microscopic immunoperoxidase labeling of tyrosine hydroxylase and glutamic acid decarboxylase immunoreactivity in the rat medial preoptic area.
An improved gold-substituted silver intensification procedure for the peroxidase-diaminobenzidine (DAB) reaction product was developed. The method was applied in the rat medial preoptic area to label tyrosine hydroxylase (TH)-immunoreactive profiles. Following the gold toning, the same sections were immunostained for glutamic acid decarboxylase (GAD) immunoreactivity with non-intensified peroxidase-DAB. Single DAB-labeled GAD axons were found in symmetric synaptic connection with unlabeled dendrites as well as with gold-toned immunoperoxidase-containing TH neurons.
D015100:3,3'-Diaminobenzidine; D000818:Animals; D001560:Benzidines; D005260:Female; D005968:Glutamate Decarboxylase / Q000032:analysis* / Q000276:immunology; D006046:Gold; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D011301:Preoptic Area / Q000201:enzymology*; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012834:Silver; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis* / Q000276:immunology
D001560:Benzidines; D015100:3,3'-Diaminobenzidine; D012834:Silver; D006046:Gold; D011388:Prolactin; D014446:Tyrosine 3-Monooxygenase; D005968:Glutamate Decarboxylase