[Elevated immunoreactive-somatostatin levels in the brain of ataxic mutant mice].
Immunoreactive-somatostatin (IR-SRIF) levels were investigated in the brain of 4 types of ataxic mice (Rolling Mouse Nagoya, Weaver, PCD, Staggerer) with different cerebellar pathologies. IR-SRIF concentrations (ng/mg) were found to be significantly elevated in both cerebellum and cerebrum of all ataxic mutant mice, IR-SRIF (ng/organ) was found to be increased in the cerebellum and cerebrum in Rolling Mouse Nagoya and PCD compared with control mice. The gel-filtration profile (Sephadex G-50) in the cerebellar extracts of Rolling Mouse Nagoya proved to be identical to that of control mice. Three peaks of IR-SRIF were found to be uniformly elevated in Rolling Mouse Nagoya, with the highest peak coinciding with authentic somatostatin-14. The present results suggest that elevated levels of IR-SRIF in the brain may play a role in the mechanism underlying the manifestation of ataxia in ataxic mutant mice, especially in Rolling Mouse Nagoya and PCD.
D000818:Animals; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry; D002524:Cerebellar Ataxia / Q000378:metabolism / Q000662:veterinary*; D002531:Cerebellum / Q000473:pathology; D066298:In Vitro Techniques; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000378:metabolism*; D009929:Organ Size; D013004:Somatostatin / Q000032:analysis* / Q000276:immunology
D013004:Somatostatin