title: Effect of vasoactive intestinal peptide, somatostatin, neurotensin, cholecystokinin octapeptide, and secretin on intestinal absorption of amino acid in rat.
abstract: The effects of vasoactive intestinal peptide (VIP), somatostatin (SRIF), neurotensin (NT), cholecystokinin octapeptide (CCK-8), and secretin (SEC) on the intestinal absorption of amino acid were investigated. Six groups of Wistar rats were studied: (1) controls; (2) VIP treated; (3) SRIF treated; (4) NT treated; (5) CCK-8 treated; (6) SEC treated. [3H]Leucine was given intraluminally through a cannula at the ligament of Treitz, a number of blood samples were obtained through a superior mesenteric vein catheter 1-60 min after administration of [3H]leucine, and the radioactivity of plasma was measured to evaluate the absorption of [3H]leucine. It was shown that VIP and SRIF significantly inhibited the absorption of [3H]leucine (by 59.1% and 38.7%, respectively), whereas NT, CCK-8, and SEC significantly enhanced absorption (by 44.2%, 49.6%, and 39.1%, respectively). Radioimmunoassays of VIP, SRIF, and NT showed that at least some of the hormones or peptides exerted their effects on absorption of leucine at or near their physiological concentrations.
mesh_terms: D000818:Animals; D005260:Female; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects*; D007930:Leucine / Q000378:metabolism*; D008297:Male; D009496:Neurotensin / Q000097:blood / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000494:pharmacology; D012844:Sincalide / Q000494:pharmacology; D013004:Somatostatin / Q000097:blood / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000097:blood / Q000494:pharmacology
chemical_list: D005768:Gastrointestinal Hormones; D010455:Peptides; D012633:Secretin; D014660:Vasoactive Intestinal Peptide; D009496:Neurotensin; D013004:Somatostatin; D007930:Leucine; D012844:Sincalide