Occurrence of mannose resistant hemagglutinins in Escherichia coli strains isolated from porcine colibacillosis.
Three-hundred and fifty-eight E. coli strains isolated from piglets were tested for the presence of hemagglutinins by the use of the active hemagglutination test with or without mannose. Additionally 86 strains from the mentioned number of strains were investigated for the presence of common fimbriae using the same method but growing the strains in media especially suited for the development of this kind of fimbriae. These 358 strains and additionally 202 E. coli strains were tested using antisera for 987P and K88 antigens. It was found, using the active hemagglutination test, that 51.4% of the strains were hemagglutinating. The hemagglutinating strains carried the K88 antigen. All these strains were isolated from new-born and weaned piglets with enterotoxic form of colibacillosis, called also E. coli diarrhea. From cases of this form of colibacillosis originated also 26.7% of the strains in which common fimbriae (type 1) were detected. This result was obtained when the BHI medium was used for cultivation. In case of TSA medium only 2.3% of strains were positive. No specific or common fimbriae were found in strains recovered from septic form of colibacillosis and oedema disease (called also enterotoxaemic form of colibacillosis). No strain of 560 examined showed the presence of fimbrial 987P antigen.
D000372:Agglutination Tests; D000818:Animals; D000833:Animals, Suckling; D000942:Antigens, Bacterial / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D003470:Culture Media; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000276:immunology / Q000472:pathogenicity* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000032:analysis / Q000276:immunology; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000032:analysis*; D008358:Mannose / Q000494:pharmacology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D003470:Culture Media; D029968:Escherichia coli Proteins; D006388:Hemagglutinins; C026888:K88 antigen, E coli; D036781:Fimbriae Proteins; D008358:Mannose