Kinetic parameters of tyrosine hydroxylase in the rat's preoptic region during sleep deprivation and recovery induced by ambient temperature.
The role of catecholaminergic mechanisms in determining the changes in the rat's preoptic cyclic adenosine monophosphate (cAMP) concentration during sleep deprivation and recovery induced by ambient temperature was investigated in the present study. To this end, the activity of tyrosine hydroxylase, the rate-limiting enzyme in catecholamine biosynthesis, was measured in the preoptic region of rats maintained in: (a) control (22 degrees C for 52 h), (b) deprivation (-10 degrees C for 52 h), and (c) recovery (22 degrees C for 4 h after 48 h at -10 degrees C) conditions. The enzyme followed a Michaelis-Menten kinetic. The analysis of substrate-related kinetic parameters (Km and Vmax) did not show any clear-cut difference between experimental conditions, which, as already known, induce both sleep deprivation and recovery in relation to significant cAMP changes.
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D011301:Preoptic Area / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012892:Sleep Deprivation / Q000502:physiology*; D013696:Temperature; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D000242:Cyclic AMP; D014446:Tyrosine 3-Monooxygenase