In vitro release of somatostatin from cerebral cortical slices: characterization of electrically evoked release.
Calcium-dependent, tetrodotoxin (1 microM)-sensitive release of somatostatin-like immunoreactivity (SRIF-LI) could be evoked by electrical field stimulation of vibratome-cut cerebral cortical slices superfused in vitro. The release of SRIF-LI from cortical slices was frequency-dependent, and showed facilitation between 5 and 25 Hz. Release was also current-dependent and above a threshold of 10 mA increased to plateau at 80 mA. Addition of peptidase inhibitors did not improve the recovery of SRIF-LI, and HPLC analysis of the released material showed that over 95% of SRIF-LI released corresponded to SRIF-14 (SRIF-28).
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D004558:Electric Stimulation; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013003:Somatosensory Cortex / Q000502:physiology; D013004:Somatostatin / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D013779:Tetrodotoxin; D013004:Somatostatin; D002118:Calcium