Rapid diagnosis of whooping cough using monoclonal antibody.
A counterimmunoelectrophoresis (CIE) method for antigen detection using monoclonal antibody was assessed for its ability to aid in the rapid diagnosis of Bordetella pertussis in 59 patients. A positive diagnosis from a combination of results from tests of serum and urine was obtained in 51 (87%) of cases. For sera, CIE had a sensitivity of 85% and a specificity of 94%; for urine samples the sensitivity was 81% and a specificity of 100%. Antigen detection by CIE is simple to perform and yields results on the same day, thus allowing treatment to begin at an early stage.
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis; D001886:Bordetella pertussis / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003378:Counterimmunoelectrophoresis; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D013997:Time Factors; D014917:Whooping Cough / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial