Sodium regulation of agonist and antagonist binding to beta-adrenoceptors in intact and Ns-deficient membranes.
Agonist binding to various hormone receptors mediating adenylate cyclase inhibition is decreased by sodium ions. We studied the influence of Na+ on agonist and antagonist binding to beta-adrenoceptors in membrane preparations of guinea pig lung, S49 lymphoma wild-type cells (WT) and their Ns-deficient cyc- variants by measuring binding of the antagonist, [125I]iodocyanopindolol [( 125I]CYP). At 37 degrees C, sodium decreased the receptor affinity for the agonist, isoproterenol, in all three membrane preparations. In lung and WT membranes, Na+ steepened the shallow agonist competition curves in a manner similar to and synergistic with guanine nucleotides. When binding was performed at 4 degrees C, sodium regulation but not guanine nucleotide regulation of agonist binding was preserved. At the low temperature, [125I]CYP affinity was reduced, and sodium increased [125I]CYP binding in both Ns-containing and Ns-deficient membranes by increasing the antagonist affinity without significant change in total receptor number. Compared to Na+, Li+ and K+ were much less potent and efficient in decreasing agonist and increasing antagonist binding. Na+ and Mg2+ had opposite effects on agonist binding in the Ns-containing lung and WT membranes but not in the Ns-deficient cyc- membranes. The data indicate that sodium not only regulates binding of inhibitory hormone receptors but also agonist and antagonist binding to the adenylate cyclase stimulatory beta-adrenoceptor. The finding that sodium regulation of beta-adrenoceptor binding is also observed in the Ns (alpha s)-deficient cyc- membranes, furthermore, indicates that the target of sodium is not the alpha-subunit of Ns but possibly a component common to both types of receptor systems regulating adenylate cyclase activity.
D000318:Adrenergic beta-Agonists / Q000378:metabolism*; D000319:Adrenergic beta-Antagonists / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004347:Drug Interactions; D019204:GTP-Binding Proteins / Q000172:deficiency*; D006150:Guanine Nucleotides / Q000378:metabolism; D006168:Guinea Pigs; D008168:Lung / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000032:analysis / Q000187:drug effects*; D012964:Sodium / Q000494:pharmacology*
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D006150:Guanine Nucleotides; D011943:Receptors, Adrenergic, beta; D012964:Sodium; D019204:GTP-Binding Proteins; D008274:Magnesium