Use of the inhibition of enzymatic antioxidant systems in order to evaluate their physiological importance.
Chemical inhibitors of the different antioxidant enzymes were systematically testet either on purified enzymes of after incubation with human fibroblasts in culture. Inhibition values were obtained for catalase with aminotriazole, for superoxide dismutase with diethyldithiocarbamate, for glutathione peroxidase with mercaptosuccinate, for glutathione reductase with bischloroethylnitrosourea and for glutathione synthesis with buthionine sulfoximine. Viability of cells incubated with these inhibitors was then tested under normal conditions and under high oxygen pressure; the data were correlated with the above-mentioned inhibitory values. Cell viability was particularly affected when the glutathione-related enzymes, especially glutathione peroxidase, were inhibited.
D000640:Amitrole / Q000494:pharmacology; D019328:Buthionine Sulfoximine; D002330:Carmustine / Q000494:pharmacology; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D004050:Ditiocarb / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology*; D005721:Glutamate-Cysteine Ligase / Q000037:antagonists & inhibitors; D005978:Glutathione / Q000096:biosynthesis*; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors* / Q000502:physiology; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors* / Q000502:physiology; D006801:Humans; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors* / Q000502:physiology; D013870:Thiomalates / Q000494:pharmacology
D013870:Thiomalates; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; C046062:2-thiomalic acid; D004050:Ditiocarb; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; D002330:Carmustine; D000640:Amitrole