Chromosomal localization of the mouse homolog of the Huntington's disease linked G8 (D4S10) marker.
The human DNA probe G8 defines D4S10, a polymorphic locus tightly linked to the Huntington's disease gene on human chromosome 4. A subclone of G8, pSC33, showed significant cross-hybridization to discrete restriction fragments in total genomic mouse DNA. The probe detected restriction fragment length polymorphisms (RFLPs) with the enzymes Taq I and Msp I, permitting chromosomal localization of the mouse G8 homolog by linkage analysis using three sets of recombinant inbred mouse strains: BXH, BXD, and AKXD. The mouse locus was mapped to the central region of chromosome 11 at 1 centiMorgan from the SPARC gene, a locus whose human counterpart is on human chromosome 5.
D000818:Animals; D002874:Chromosome Mapping; D006816:Huntington Disease / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid