title: Effects of organic solvents and orthophosphate on the ATPase activity of F1 ATPase.
abstract: The ATPase activity of soluble F1 ATPase of mitochondria is activated by Pi. The concentration of Pi required for half-maximal activation decreases from a value higher than 50 mM to about 1 mM Pi when one of the organic solvents dimethyl sulfoxide (15 to 30%), methanol (7.5 to 15%) or ethylene glycol (10 to 30%) is added to the assay medium. This effect is observed in the presence of MgCl2 but not in the presence of CaCl2.
mesh_terms: D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002417:Cattle; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D000432:Methanol / Q000494:pharmacology; D008929:Mitochondria, Heart / Q000201:enzymology*; D010710:Phosphates / Q000494:pharmacology*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology*
chemical_list: D005026:Ethylene Glycols; D010710:Phosphates; D012997:Solvents; D015636:Magnesium Chloride; D006180:Proton-Translocating ATPases; D019855:Ethylene Glycol; D008274:Magnesium; D002122:Calcium Chloride; D000432:Methanol; D004121:Dimethyl Sulfoxide