Localization and identification of an Entamoeba histolytica adhesin.
The adherence of Entamoeba histolytica trophozoites to target cells was studied by using monoclonal antibodies (MAbs) and adhesion-deficient mutants of the parasite. MAbs Adh-1 and Adh-2 reacted with a surface protein of approximately 112 kDa of the total proteins of trophozoites from the wild type strain, clone A, strain HM1:IMSS. Both MAbs reacted weakly with the adhesion-deficient mutant clones, C-98, C-919 and C-923, all derived from HM1:IMSS. MAbs Adh-1 and Adh-2 incubated with trophozoites from clone A inhibited adherence to red blood cells, erythrophagocytosis and cytopathic effect on cell culture monolayers. Antibodies against a approximately 112 kDa polypeptide were found in the sera from patients with hepatic abscess. These results demonstrate that the adherence of trophozoites to target cells is a necessary event in order for cytopathogenicity to occur.
D000268:Adhesiveness; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000502:physiology; D000953:Antigens, Protozoan / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects*; D004748:Entamoeba histolytica / Q000472:pathogenicity / Q000502:physiology; D004912:Erythrocytes / Q000502:physiology; D006801:Humans; D037102:Lectins; D008101:Liver Abscess, Amebic / Q000097:blood / Q000276:immunology; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D009154:Mutation; D010587:Phagocytosis; D015800:Protozoan Proteins
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D037102:Lectins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; C052452:galactose inhibitable adherence protein, Entamoeba histolytica