Oxygen toxicity in the infant rhesus monkey: effects on regulatory peptides in lung and blood.
A total of ten 6-month-old male rhesus monkey (Macaca mulatta) infants, born full-term, were positive-pressure ventilated with greater than 95% oxygen or room air (controls). A protocol was used which closely simulated pediatric intensive care. To test if regulatory peptides were affected by the oxygen treatment, and to search for an early marker of oxygen toxicity, lung tissue samples and systemic mixed venous blood were collected at 6, 12 and 24 hours after onset of treatment. The peptides, gastrin releasing peptide (GRP), calcitonin gene-related peptide (CGRP), peptide YY (PYY), vasoactive intestinal peptide (VIP) and somatostatin (SOM), were quantitated in lung tissue extracts and plasma using radioimmunoassay. Lung tissue GRP, CGRP, and PYY levels appeared to decrease gradually with time, perhaps as a result of the positive pressure ventilation procedure. GRP and CGRP levels decreased less among monkey infants ventilated with oxygen, thus they were significantly higher at 24 hours than in air ventilated controls. VIP levels were significantly lower among tests compared to controls at that time. Blood peptide levels did not change with oxygen treatment. These results suggest that tissue concentrations of certain pulmonary regulatory peptides can become altered by ventilation with greater than 95% oxygen. A blood borne peptide marker was not identified.
D000818:Animals; D000831:Animals, Newborn; D001997:Bronchopulmonary Dysplasia / Q000097:blood*; D019886:Gastrin-Releasing Peptide; D006801:Humans; D007231:Infant, Newborn; D008168:Lung / Q000378:metabolism*; D008253:Macaca mulatta; D008297:Male; D010102:Oxygen Inhalation Therapy / Q000009:adverse effects*; D019894:Peptide YY; D010455:Peptides / Q000097:blood*; D011863:Radioimmunoassay; D013004:Somatostatin / Q000097:blood; D014660:Vasoactive Intestinal Peptide / Q000097:blood
D010455:Peptides; D019894:Peptide YY; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D019886:Gastrin-Releasing Peptide