Structural characterization of peptides derived from prosomatostatins I and II isolated from the pancreatic islets of two species of teleostean fish: the daddy sculpin and the flounder.
The primary structures of three peptides from extracts from the pancreatic islets of the daddy sculpin (Cottus scorpius) and three analogous peptides from the islets of the flounder (Platichthys flesus), two species of teleostean fish, have been determined by automated Edman degradation. The structures of the flounder peptides were confirmed by fast-atom bombardment mass spectrometry. The peptides show strong homology to residues (49-60), (63-96) and (98-125) of the predicted sequence of preprosomatostatin II from the anglerfish (Lophius americanus). The amino acid sequences of the peptides suggest that, in the sculpin, prosomatostatin II is cleaved at a dibasic amino acid residue processing site (corresponding to Lys61-Arg62 in anglerfish preprosomatostatin II). The resulting fragments are further cleaved at monobasic residue processing sites (corresponding to Arg48 and Arg97 in anglerfish preprosomatostatin II). In the flounder the same dibasic residue processing site is utilised but cleavage at different monobasic sites takes place (corresponding to Arg50 and Arg97 in anglerfish preprosomatostatin II). A peptide identical to mammalian somatostatin-14 was also isolated from the islets of both species and is presumed to represent a cleavage product of prosomatostatin I.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000378:metabolism*; D005412:Flatfishes / Q000378:metabolism*; D005432:Flounder / Q000378:metabolism*; D007515:Islets of Langerhans / Q000032:analysis*; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000302:isolation & purification*; D011487:Protein Conformation; D011498:Protein Precursors / Q000032:analysis*; D013004:Somatostatin / Q000032:analysis*; D013045:Species Specificity
D000596:Amino Acids; D010446:Peptide Fragments; D011498:Protein Precursors; D013004:Somatostatin; C028456:prosomatostatin