Induction of chromosomal aberrations in human cells by a temperature-sensitive mutant of herpes simplex virus type 2 and its revertants.
The induction of chromosomal aberrations by a temperature-sensitive (ts) mutant of herpes simplex virus type 2 (HSV-2) strain Hg52 (ts 13), its revertants 4-8 and 5-8 and by etalon strains HSV-1 17 syn+ and HSV-2 Hg52 was studied in human fibroblast and lymphocyte cultures. The effect on chromosomes of the revertants was tested at permissive (31 degrees C) and non-permissive (38 degrees C) temperatures. At 38 degrees C the revertants could not induce DNase activity. The present results contribute to the possible role of a herpes-coded nuclease in induction of chromosomal aberrations.
D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009154:Mutation; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013696:Temperature
D003851:Deoxyribonucleases