Peroxidase labelled mumps virus antigens and their application in IgM capture immunoassay: first experience.
Three types of mumps virus antigen have been prepared: virion antigen (MV); soluble nucleoprotein (NP) antigen; envelope glycoprotein (eGp) antigen. These antigens were coupled directly with horse-raddish peroxidase and tested by IgM-capture technique against selected human sera. The advantage of the suggested design, in addition to general superiority of the IgM-capture systems, lies in its selectivity achieved by introducing a monoclonal antibody for IgM binding and in time saving due to the use of labelled antigen without the loss of sensitivity and specificity. The assay fulfils the criteria for quick viral diagnostic. Our first experience on application of the labelled antigens in enzyme immunoassay (EIA) is described.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D002642:Chick Embryo; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*; D009109:Mumps virus / Q000276:immunology*; D009698:Nucleoproteins / Q000276:immunology; D011237:Predictive Value of Tests; D014759:Viral Envelope Proteins / Q000276:immunology; D014771:Virion / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M; D009698:Nucleoproteins; D014759:Viral Envelope Proteins