Radiation inactivation probe of membrane-bound enzymes: gamma-glutamyltranspeptidase, aminopeptidase N, and sucrase.
gamma-Glutamyltranspeptidase (GGT), aminopeptidase N (AP-N), and sucrase in purified rabbit intestinal brush border membrane vesicles were irradiated in situ at -135 degrees C using high energy electrons. Surviving activities of the enzymes were measured as a function of radiation dose, and the functional unit target sizes (corresponding to carbohydrate-free polypeptides) were determined using target analysis. The in situ functional unit sizes were GGT 59 kDa, AP-N 59 kDa, and sucrase 63 kDa. Together with biochemical data determined previously, it is concluded that the noncovalently attached large (approximately 40 kDa) and small (approximately 25 kDa) subunits of GGT are both required for catalytic activity. Furthermore, these data suggest that (i) the membrane-bound form of AP-N consists of one or more noncovalently attached subunits of 59 kDa, each of which is enzymatically active; and (ii) in situ sucrase activity is associated with a subunit of 63 kDa which is noncovalently attached within the sucrase-isomaltase complex.
D000626:Aminopeptidases / Q000528:radiation effects*; D000818:Animals; D018826:CD13 Antigens; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000528:radiation effects; D008871:Microvilli / Q000201:enzymology / Q000528:radiation effects; D011487:Protein Conformation; D011817:Rabbits; D013393:Sucrase / Q000528:radiation effects*; D005723:gamma-Glutamyltransferase / Q000528:radiation effects*
D005723:gamma-Glutamyltransferase; D013393:Sucrase; D000626:Aminopeptidases; D018826:CD13 Antigens