title: Clostridium spiroforme toxin is a binary toxin which ADP-ribosylates cellular actin.
abstract: We have purified from Clostridium spiroforme strain 246 an heterogeneous population of proteins (Sa) ranging from 43 to 47 kilodaltons exhibiting ADP-ribosyl transferase activity as do C. botulinum C2 toxin component I or the ia chain of C. perfringens E iota toxin. C. spiriforme Sa had alone no activity upon injection in mice or inoculated to Vero cells. When spiroforme ADP ribosyl transferase were mixed with a trypsin activated protein (Sb) separated from C. spiroforme bacterial supernatant, a lethal effect in mice and cytotoxicity on Vero cells were recorded. The Sa cross-reacted immunologically with either the light chain of C. perfringens E iota toxin or the ADP-ribosyl transferase from C. difficile 196 strain. No immunological relatedness was observed between Sa and C2 toxin component I. C. spiroforme toxin is thus another binary toxin close to iota.
mesh_terms: D036002:ADP Ribose Transferases; D000199:Actins / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis / Q000494:pharmacology*; D002460:Cell Line; D002846:Chromatography, Affinity; D003016:Clostridium perfringens / Q000201:enzymology; D003429:Cross Reactions; D007525:Isoelectric Focusing; D051379:Mice; D010430:Pentosyltransferases / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D000199:Actins; D001427:Bacterial Toxins; C050737:iota toxin, Clostridium perfringens; C054948:iota toxin, Clostridium spiroforme; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; D010430:Pentosyltransferases