title: Cytotoxicities of sodium benzoate in primary culture of hepatocytes from adult rat liver.
abstract: The cytotoxicities of sodium benzoate was studied using primary culture of hepatocytes established from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in serum-free culture medium. The activities of ornithine transcarbamylase (as a marker of mitochondria) and tyrosine aminotransferase (as a marker of cytosol) were clearly suppressed by sodium benzoate at concentration in excess of 500 micrograms/ml. Intracellular protein synthesis and DNA synthesis were also suppressed, and the suppression of DNA synthesis was observed even with a lower concentration of benzoate (100 micrograms/ml).
mesh_terms: D000818:Animals; D001565:Benzoates / Q000633:toxicity*; D019817:Benzoic Acid; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D014444:Tyrosine Transaminase / Q000378:metabolism
chemical_list: D001565:Benzoates; D019817:Benzoic Acid; D004247:DNA; D009954:Ornithine Carbamoyltransferase; D014444:Tyrosine Transaminase