Fluid volumes in rainbow trout, Salmo gairdneri: application of compartmental analysis.
1. The measurement of fluid volumes by the indicator dilution technique and compartmental analysis was re-evaluated in free-swimming, undisturbed rainbow trout. 2. Plasma (33.5 ml/kg body wt) and blood (41.3 ml/kg body wt) volumes estimated by compartmental analysis from blood samples taken early (less than 5 min) after dye injection were 40% lower than volumes calculated by sampling late (greater than or equal to 80 min). 3. The rate of exchange of dye between plasma and interstitial fluid was high (48%/hr) compared to mammals (5%/hr) which supports the hypothesis that teleost capillaries have high protein permeability. 4. Total extracellular volume estimated using a single pool model (210.5 ml/kg body wt) of inulin kinetics was 20% higher than that calculated by a three pool model (172.8 ml/kg body wt).
D000818:Animals; D005070:Evans Blue; D005110:Extracellular Space / Q000378:metabolism*; D005260:Female; D007444:Inulin; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D010953:Plasma Volume; D012487:Salmonidae / Q000502:physiology*; D014337:Trout / Q000502:physiology*
D005070:Evans Blue; D007444:Inulin