title: Techniques to measure DNA single-strand breaks in cells: a review.
abstract: Alkaline sucrose sedimentation was for a number of years the standard procedure for the measurement of single-strand breaks. Some years ago a number of new techniques with improved sensitivity were introduced. The following techniques are presented and discussed: alkaline unwinding, alkaline filter elution, nucleoid sedimentation, viscoelastometry, microelectrophoresis of single cells, DNA precipitation, pulse field gel electrophoresis, fluctuation spectroscopy and nick translation.
mesh_terms: D004249:DNA Damage; D004277:DNA, Single-Stranded; D005821:Genetic Techniques
chemical_list: D004277:DNA, Single-Stranded