Dopamine and apomorphine do not modulate the uptake of [3H]D-aspartate in the rat striatum in-vitro.
Sodium-dependent [3H]D-aspartate uptake was measured in rat striatal homogenates. The uptake was inhibited by both L- and D-glutamate, with IC50 values of 5.6 and 224 microM, respectively. Dopamine (10(-7)-10(-4) M), apomorphine (10(-7) M), sulpiride (10(-6) M) or a combination of dopamine and sulpiride were found not to affect the observed uptake of [3H]D-aspartate. Thus, the in-vitro dopaminergic modulation of high affinity glutamate uptake reported in the literature is not found when [3H]D-aspartate is used instead of [3H]L-glutamate.
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D001224:Aspartic Acid / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001224:Aspartic Acid; D001058:Apomorphine; D004298:Dopamine