Dynein isoforms in sea urchin eggs.
Biochemical and immunological analysis of unfertilized sea urchin eggs has revealed the presence of at least two distinct isoforms of cytoplasmic dyneins, one soluble and the other microtubule-associated. The soluble enzyme is a 20 S particle with a MgATPase activity that can be activated 5-fold by nonionic detergents. It contains heavy chain polypeptides that 1) comigrate with the dynein heavy chains of embryonic cilia; 2) cross-react with antibodies against flagellar dynein; and 3) are cleaved by UV irradiation in the presence of MgATP and sodium vanadate into specific peptide fragments. The soluble egg dynein is, therefore, closely related to axonemal dynein and may be a ciliary precursor. Egg microtubule preparations contain a distinct dynein-like polypeptide, previously designated HMr-3 (Scholey, J.M., Neighbors, B., McIntosh, J.R., and Salmon, E.D. (1984) J. Biol Chem. 259, 6516-6525). HMr-3 binds microtubules in an ATP-sensitive fashion; it sediments at 20 S on sucrose density gradients, and it is susceptible to vanadate-sensitized UV cleavage. However, HMr-3 can be distinguished from the soluble cytoplasmic dynein on the basis of its weak cross-reactivity with antiflagellar dynein antibodies, its heavy chain composition on high resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis, its low specific ATPase activity, and the molecular weight of its vanadate-induced UV cleavage fragments. HMr-3 may represent a dynein-like polypeptide that is distinct from the pool of ciliary dynein precursors.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification*; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D004398:Dyneins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008869:Microtubule-Associated Proteins / Q000276:immunology / Q000302:isolation & purification; D008870:Microtubules / Q000187:drug effects / Q000201:enzymology; D010063:Ovum / Q000201:enzymology*; D017239:Paclitaxel; D012617:Sea Urchins; D014638:Vanadates / Q000494:pharmacology
D000470:Alkaloids; D007527:Isoenzymes; D008869:Microtubule-Associated Proteins; D014638:Vanadates; D000251:Adenosine Triphosphatases; D004398:Dyneins; D017239:Paclitaxel