Identification of the tridecapeptide dynorphin B (rimorphin) within perikarya of rat duodenum.
Using an immunofluorescence microscopic staining technique, the opioid peptide dynorphin B (rimorphin) was revealed within neuronal cell bodies of the rat duodenum. Dynorphin B immunoreactive perikarya were revealed in the myenteric and submucousal plexus as well as in the longitudinal muscle layer. They were large in diameter and round in shape and they contained a large round nucleus. Because no dynorphin B immunofluorescent nerve fibre and terminal could be noted it might be that dynorphin B is further cleaved by proteases into the bioactive opioid pentapeptide Leu-enkephalin and dynorphin B(6-13). These findings might also indicate that dynorphin B is processed within duodenal perikarya and that it has important physiological roles in the rat duodenum.
D000818:Animals; D004386:Duodenum / Q000294:innervation*; D004399:Dynorphins / Q000031:analogs & derivatives* / Q000032:analysis; D004723:Endorphins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D008297:Male; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D004723:Endorphins; D004399:Dynorphins; C036200:rimorphin