A flexible peptide spacer increases the efficacy of holoricin anti-T cell immunotoxins.
Immunotoxins, constructed by chemically cross-linking an antibody and protein toxin, do not possess the high efficacy of the native toxin. Decreases in toxicity are due in part to the steric constraints imposed on the two macromolecules, which result in both decreased antibody binding and toxin function. In examining the structural features that influence efficacy in holotoxin-antibody conjugates, it was found that the incorporation of a 29-residue polypeptide, derived from the insulin B chain between the antibody and ricin moiety, resulted in an increase in both potency and efficacy. In a murine model system, potency of the peptide spacer conjugate was increased nearly 10-fold; however, when examined by the procedure used to purge bone marrow, the peptide spacer conjugate was not demonstrably more toxic to nontarget cells than the nonspacer conjugate. Thus, in addition to increases of efficacy and potency, this novel immunotoxin demonstrated increased specificity by approximately 10-fold. To test the general utility of peptide spacer inclusion, a T101-ricin conjugate was constructed with the peptide spacer. It yielded a protein synthesis inhibition rate of -0.6 log/h on MOLT-3 cells, greater than 10-fold more efficacious than a previously constructed nonspacer T101-ricin conjugate examined under similar conditions.
D000911:Antibodies, Monoclonal / Q000633:toxicity*; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D000922:Immunotoxins / Q000633:toxicity*; D007700:Kinetics; D010455:Peptides / Q000276:immunology*; D011487:Protein Conformation; D012276:Ricin / Q000276:immunology* / Q000633:toxicity; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000922:Immunotoxins; D010455:Peptides; D018800:Thy-1 Antigens; D012276:Ricin