Mid to late S-phase replication of the nucleolus in lymphoid human Molt-4 cells.
Indirect immunofluorescence staining of synchronized lymphoid human Molt-4 cells with proliferating cell nuclear antigen autoantibodies specific for cyclin revealed nucleolar staining only in cells in mid to late S-phase. These results together with similar earlier observations in epithelial and fibroblasts cells indicate that this organelle replicates in mid to late S-phase in cultured somatic cells.
D001323:Autoantibodies / Q000032:analysis; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D007399:Interphase; D008221:Lymphoid Tissue / Q000166:cytology*; D009687:Nuclear Proteins / Q000276:immunology; D018809:Proliferating Cell Nuclear Antigen
D001323:Autoantibodies; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen