Occurrence and possible roles of acetoacetyl-CoA thiolase and 3-ketoacyl-CoA thiolase in peroxisomes of an n-alkane-grown yeast, Candida tropicalis.
Two kinds of 3-ketoacyl-CoA thiolases were found in the peroxisomes of Candida tropicalis cells grown on n-alkanes (C10-C13). One was a typical acetoacetyl-CoA thiolase specific only to acetoacetyl-CoA, while another was 3-ketoacyl-CoA thiolase showing high activities on the longer chain substrates. A high level of the latter thiolase activity in alkane-grown cells was similar to that of other enzymes constituting the fatty acid beta-oxidation system in yeast peroxisomes. These facts suggest that the complete degradation of fatty acids to acetyl-CoA is carried out in yeast peroxisomes by the cooperative contribution of acetoacetyl-CoA thiolase and 3-ketoacyl-CoA thiolase.
D000105:Acetyl Coenzyme A / Q000031:analogs & derivatives / Q000378:metabolism; D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism*; D000102:Acetyl-CoA C-Acyltransferase / Q000378:metabolism*; D000123:Acetyltransferases / Q000378:metabolism*; D000214:Acyl Coenzyme A; D000217:Acyltransferases / Q000378:metabolism*; D000473:Alkanes / Q000494:pharmacology*; D002175:Candida / Q000201:enzymology* / Q000648:ultrastructure; D005227:Fatty Acids / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology*; D010084:Oxidation-Reduction
D000214:Acyl Coenzyme A; D000473:Alkanes; D005227:Fatty Acids; D007527:Isoenzymes; C010667:acetoacetyl CoA; D000105:Acetyl Coenzyme A; D000217:Acyltransferases; D000123:Acetyltransferases; D000102:Acetyl-CoA C-Acyltransferase; D000101:Acetyl-CoA C-Acetyltransferase