Identification of HLA-DP polymorphism with DP alpha and DP beta probes and monoclonal antibodies: correlation with primed lymphocyte typing.
Thirty-four lymphoblastoid cell lines that had been previously typed for HLA-DP antigens by primed lymphocyte typing (PLT) were tested by Southern blotting and by ELISA. Using two DP beta probes and a DP alpha probe with a series of enzymes, it is possible to identify restriction fragment length polymorphism (RFLP) patterns characteristic of DPw1, -2, -3, -4, and possibly -5. ELISA typing results, based on two polymorphic DP antibodies DP11.1 and ILR1, were compared with PLT-defined and RFLP-defined types. Thus, using a range of probes and enzymes it is possible to identify DP polymorphism. The value of monoclonal antibodies for such studies is demonstrated, and the molecular data can, in some cases, pinpoint the amino acids responsible for the specificity of the monoclonal antibodies.
D000483:Alleles; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D004797:Enzyme-Linked Immunosorbent Assay; D005819:Genetic Markers; D006681:HLA-D Antigens / Q000235:genetics*; D006682:HLA-DP Antigens / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D000911:Antibodies, Monoclonal; D004274:DNA, Recombinant; D005819:Genetic Markers; D006681:HLA-D Antigens; D006682:HLA-DP Antigens; D004247:DNA