title: Nucleotide sequence of the Azospirillum brasilense Sp7 glutamine synthetase structural gene.
abstract: The complete nucleotide sequence of the glnA gene, encoding the glutamine synthetase subunit of Azospirillum brasilense Sp7, was established. This is the first Azospirillum gene sequenced. The gene encodes a 468 residue polypeptide of MW 51,917. The similarity coefficient (SAB) between the polypeptidic sequence of Azospirillum and Anabaena 7120, which is the only other glnA sequence available, is 58%. No significant homology with E. coli canonical and ntr promoters, or with the promoter region of the Anabaena glnA gene was found. When fused to an E. coli promoter, the gene could be translated in E. coli, despite a very biased codon usage and an atypical Shine-Dalgarno sequence.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D000458:Cyanobacteria / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D005796:Genes; D005798:Genes, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000235:genetics; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D005974:Glutamate-Ammonia Ligase