title: Active site studies on a narrow-specificity thyroliberin-hydrolysing pyroglutamate aminopeptidase purified from synaptosomal membrane of guinea-pig brain.
abstract: The effect of protein-modifying reagents on the activity of a purified preparation of a thyroliberin-hydrolysing pyroglutamate aminopeptidase, solubilised from synaptosomal membranes of guinea-pig brain by treatment with papain, was investigated. The results indicated that tyrosine, histidine, arginine, and possibly lysine residues were necessary for expression of catalytic activity and that these tyrosine, histidine, and arginine residues were probably located at the active site of the enzyme. Cysteine, serine, glutamate, and aspartate residues were not involved in the expression of catalytic activity.
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D006168:Guinea Pigs; D007093:Imidazoles / Q000494:pharmacology; D007700:Kinetics; D010658:Phenylglyoxal / Q000494:pharmacology; D011751:Pyroglutamyl-Peptidase I / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012395:Rose Bengal / Q000494:pharmacology; D013574:Synaptosomes / Q000201:enzymology*; D013774:Tetranitromethane / Q000494:pharmacology; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology
chemical_list: D007093:Imidazoles; D012395:Rose Bengal; D013973:Thyrotropin-Releasing Hormone; D014302:Trinitrobenzenesulfonic Acid; D000626:Aminopeptidases; D011751:Pyroglutamyl-Peptidase I; D013774:Tetranitromethane; D004047:Diethyl Pyrocarbonate; D010658:Phenylglyoxal; C012160:N-acetylimidazole