Microdissection and microcloning of the long arm of human chromosome 7.
DNA-fragments from the region of the long arm of human chromosome 7 to which the CF-locus has been mapped recently were isolated by microdissection and microcloning. We developed a new fixation procedure resulting in inserts of 1.0-7.0 kb in length with a mean value of 2.9 kb. Regional mapping of three clones on 7q was carried out by the use of different hybrid cell lines containing fragments of human chromosome 7.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA