Isolation of the MHC genes encoding the tumour-specific class I antigens expressed on a murine fibrosarcoma.
The C3H UV-induced fibrosarcoma, 1591, is highly immunogenic and, therefore, is readily rejected when transplanted into immunocompetent syngeneic recipients. Previous analysis of 1591 with tumour-specific or H-2-reactive monoclonal antibodies revealed that this antigenicity might be due to the expression of two novel class I major histocompatibility complex (MHC) antigens. In this report we describe the molecular cloning and initial characterization of three genes which account for all of the unique serological class I reactivities observed on this tumour. These include two distinct, but highly conserved, H-2L-like genes, and a third gene the product of which bears determinants which are characteristic of both the tumour and of class I products of the H-2k haplotype. Moreover, each of these genes contains a polymorphic restriction enzyme fragment which is detected in the class I sequences of 1591 relative to normal C3H tissue. Since the expression of these polymorphic class I sequences is relevant to the immunogenicity of 1591, the mutational events by which these genes were generated may be significant to the immunobiology of this tumour.
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D005354:Fibrosarcoma / Q000235:genetics / Q000276:immunology*; D006183:H-2 Antigens / Q000235:genetics*; D006239:Haplotypes; D008285:Major Histocompatibility Complex; D051379:Mice; D008809:Mice, Inbred C3H; D009381:Neoplasms, Radiation-Induced / Q000235:genetics / Q000276:immunology; D012150:Polymorphism, Restriction Fragment Length; D014466:Ultraviolet Rays
D000951:Antigens, Neoplasm; D006183:H-2 Antigens