Structure of a methanofuran derivative found in cell extracts of Methanosarcina barkeri.
Cell extracts prepared from cells of Methanosarcina barkeri grown on hydrogen and carbon dioxide, acetate, or methanol contain a coenzyme structurally related to methanofuran. This modified coenzyme was highly purified and its structure assigned as 4-[N-(gamma-L-glutamyl-gamma-L-glutamyl-gamma-L-glutamyl-gamma-L-glutamy l)-p- (beta-amino-ethyl)phenoxymethyl]-2-(aminomethyl)furan. The key structural evidence was obtained by high-resolution fast atom bombardment-mass spectrometry and 1H NMR spectroscopy. Quantitative analysis of the hydrolytic fragments of the coenzyme supported the assigned structure. We propose that this coenzyme be called methanofuran-b.
D055598:Chemical Phenomena; D002621:Chemistry; D019605:Euryarchaeota / Q000032:analysis*; D005663:Furans / Q000302:isolation & purification*; D005971:Glutamates; D018698:Glutamic Acid; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D008970:Molecular Weight
D005663:Furans; D005971:Glutamates; D018698:Glutamic Acid; C044289:carbon dioxide reduction factor