Inhibition of N-acetylneuraminate lyase by N-acetyl-4-oxo-D-neuraminic acid.
We show that the 4-oxo analogue of N-acetyl-D-neuraminic acid strongly inhibits N-acetylneuraminate lyase (NeuAc aldolase, EC 4.1.3.3) from Clostridum perfringens (Ki = 0.025 mM) and Escherichia coli (Ki = 0.15 mM). In each case the inhibition was competitive. N-Acetyl-D-neuraminic acid; N-Acetylneuraminate lyase; N-Acetyl-D-neuraminic acid analog; 5-Acetamido-3,5-dideoxy-beta-D-manno-non-2,4-diulosonic acid; 2-Deoxy-2,3-didehydro-N-acetyl-4-oxo-neuraminic acid; Competitive inhibitor.
D001667:Binding, Competitive; D003016:Clostridium perfringens / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D009438:Neuraminic Acids / Q000494:pharmacology*; D007652:Oxo-Acid-Lyases / Q000037:antagonists & inhibitors*
C055828:N-acetyl-4-oxoneuraminic acid; D009438:Neuraminic Acids; D007652:Oxo-Acid-Lyases; C018728:N-acetylneuraminate lyase