Production of human influenza virus in a stabile line of guinea pig tongue cells expressing endogenous oncovirus: an electron microscopic study.
Guinea pig tongue (GPT) cells represent a highly sensitive host system for influenza A/WSN (H1N1) infection as evidenced by numerous ultrastructural changes, considerable production of NS1 protein and widespread budding of viral particles at the cytoplasmic membrane. Vesicles of smooth endoplasmic reticulum and of the Golgi complex were transported to the apical area of cell membrane, where the budding of virions took place. Numerous microtubules were directed vertically to these portions of plasma membrane. In contrast, maturation of the endogenous oncovirus particles occurred at the lateral cytoplasmic membrane. Beneath the area of oncovirus maturation and release, a network was seen of microfilaments oriented towards the plasma membrane. The cytoplasm of GPT cells contained numerous nonstructural protein inclusions, which evidently accumulated at the periphery of nucleoli and were seen to reach the cytoplasm crossing the pores of nuclear membrane.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003588:Cytopathogenic Effect, Viral; D003599:Cytoskeleton / Q000502:physiology / Q000648:ultrastructure; D006168:Guinea Pigs; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development*; D009858:Oncogenic Viruses / Q000254:growth & development*; D014059:Tongue / Q000166:cytology / Q000382:microbiology; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000096:biosynthesis*; D014776:Virus Cultivation; D014779:Virus Replication
D017361:Viral Nonstructural Proteins; D014764:Viral Proteins