title: Promoters and processing sites within the transforming region of bovine papillomavirus type 1.
abstract: The mRNAs present in bovine papillomavirus type 1 (BPV-1)-transformed C127 cells were studied by primer extension. The results show that two internal promoters are present in the E region of BPV-1 in addition to the previously identified promoter at coordinate 1 (H. Ahola, A. Stenlund, J. Moreno-López, and U. Pettersson, Nucleic Acids Res. 11:2639-2650, 1983). One, located at coordinate 31, generated a set of mRNAs with heterogeneous 5' ends, which may encode the major transforming protein of BPV-1, the E5 protein. The second promoter, which is located at coordinate 39, generates colinear mRNAs which encode either the E4 protein or a truncated form of the E2 protein. Unlike the cottontail rabbit papillomavirus (O. Danos, E. Georges, G. Orth, and M. Yaniv, J. Virol. 53:735-741, 1985), BPV-1 appears to lack a separate promoter for expression of the E7 protein. The major splice sites in the transforming region (E region) of the BPV-1 genome were also identified by nucleotide sequence analysis.
mesh_terms: D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D027383:Papillomaviridae / Q000235:genetics*; D011061:Poly A / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012315:RNA Caps / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D004247:DNA