Survival of Saccharomyces cerevisiae after treatment with the restriction endonuclease Alu I.
Treatment of yeast cells proficient in the repair of radiation damage (Saccharomyces cerevisiae) with the restriction endonuclease Alu I leads to a positive dose-effect relationship between inactivation level and enzyme concentration. The data suggest an uptake of the active restriction enzyme into the cells and a relationship between induction of DNA double-strand breaks and cell killing.
D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004271:DNA, Fungal / Q000187:drug effects*; D015252:Deoxyribonucleases, Type II Site-Specific; D004305:Dose-Response Relationship, Drug; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects
D004271:DNA, Fungal; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific