A model for experimental asthma: provocation in guinea-pigs immunized with Bordetella pertussis.
Guinea-pigs were sensitized with killed Bordetella pertussis (3.85 X 10(11) cells . kg-1). The presence of the immediate type of immune response was verified by passive cutaneous anaphylaxis (PCA). In anaesthetized spontaneously-breathing guinea-pigs respiration rate, tidal volume, minute ventilation, dynamic lung resistance and dynamic lung elastance were estimated on the basis of airflow velocity and pressure in the oesophagus. After provocation by a single intravenous injection of the killed cells, respiratory frequency, elastance and resistance increased but tidal volume decreased. The preventive effects of aminophylline and clemastine (50 microns . kg-1, i.v.) were observed. B. pertussis not only alters adrenergic function but provocation in B. pertussis-sensitized guinea-pigs seems to be a good model for bronchial asthma.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001249:Asthma / Q000209:etiology / Q000503:physiopathology*; D001886:Bordetella pertussis / Q000276:immunology; D001980:Bronchi / Q000503:physiopathology*; D001985:Bronchial Provocation Tests; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007114:Immunization; D010323:Passive Cutaneous Anaphylaxis; D011233:Precipitin Tests; D012129:Respiratory Function Tests
D000907:Antibodies, Bacterial