A simple and rapid method to screen for mutant mice lacking D-amino-acid oxidase activity.
A simple and rapid method to screen for mutant mice (Mus musculus) lacking D-amino-acid oxidase activity has been devised. Mice were given water containing small amounts (0.02%) of either D-methionine or D-phenylalanine. Urinary levels of the D-amino acid were examined using thin-layer chromatography. Some mice excreted substantial amounts of the D-amino acid through the urine. None of them had detectable D-amino-acid oxidase activity.
D000818:Animals; D002855:Chromatography, Thin Layer; D003605:D-Amino-Acid Oxidase / Q000172:deficiency*; D007668:Kidney / Q000201:enzymology; D008715:Methionine / Q000652:urine; D051379:Mice; D008817:Mice, Mutant Strains / Q000378:metabolism*; D010649:Phenylalanine / Q000652:urine
D010649:Phenylalanine; D008715:Methionine; D003605:D-Amino-Acid Oxidase