Tyrosine aminotransferase of chicken liver: purification and properties.
Tyrosine aminotransferase has been purified from chicken liver to homogeneity by a 5-step procedure. The resultant enzyme preparation has a specific activity (256 units activity/mg protein) comparable to results published for the enzyme purified from rat liver and represented an overall recovery of 35-40%. In terms of structure (native and subunit molecular weights, immunological reactivity, and kinetic parameters) (apparent Michaelis constants for L-tyrosine and 2-oxoglutarate, oxoacid specificity, pH optimum) the purified enzyme from chicken liver exhibits remarkable similarities to tyrosine amino-transferase from rat liver.
D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011487:Protein Conformation; D051381:Rats; D013045:Species Specificity; D014444:Tyrosine Transaminase / Q000302:isolation & purification* / Q000378:metabolism
D014444:Tyrosine Transaminase