Haplotype analysis of the human beta-globin gene complex using multiple locus specific oligonucleotide probes.
Three oligonucleotide probes complementary to specific DNA sequences of the six human globin genes (epsilon, G gamma, A gamma, psi beta, delta, beta) were synthesized. The oligonucleotides were used either singly or in combination as hybridization probes to determine the haplotype of the human beta-globin gene cluster employing the four conventionally used restriction endonucleases HincII, HindIII, AvaII, and BamHI, in addition to HpaI. Polymorphism in the epsilon- and psi beta-genes (HincII) can be simultaneously determined with a single probe mixture. One of the probes complementary to both the psi beta- and gamma-genes is useful for determining both HindIII and HincII polymorphisms. The advantages of these probes relative to conventional cDNA probes are discussed.
D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D005914:Globins / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D006868:Hydrolysis; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D015345:Oligonucleotide Probes; D005914:Globins; D004262:DNA Restriction Enzymes