title: Sea urchin actin gene linkages determined by genetic segregation.
abstract: Genetic linkage between the actin genes of Strongylocentrotus purpuratus was investigated by observing the segregation of restriction fragment length polymorphisms (RFLPs). Specific RFLPs of actin gene pairs CyI/CyIIa and CyIIIa/CyIIIb always cosegregated, confirming the linkage groups CyI-CyIIa-CyIIb previously previously determined by molecular cloning. In contrast, RFLPs of actin genes CyI/CyIIa, CyIIIa/CyIIIb, and M all segregated at random with respect to one another. This demonstrates that the known actin gene clusters CyI-CyIIa-CyIIb, CyIIIa-CyIIIb, and the M actin gene are not closely linked.
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D004247:DNA / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D006579:Heterozygote; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D012617:Sea Urchins / Q000235:genetics*
chemical_list: D000199:Actins; D004247:DNA