title: The electrophoretic isoenzyme patterns of strains of Entamoeba histolytica isolated in two major cities in Canada.
abstract: The isoenzyme patterns of 92 isolates of Entamoeba histolytica from British Columbia and 28 from Ontario were determined. Seropositivity for E. histolytica was assessed by indirect hemagglutination and enzyme-linked immunosorbent assay in the one center and by ELISA and amebic gel diffusion in the other. In both British Columbia and Ontario nonpathogenic zymodemes I and III were most common. A newly described isoenzyme pattern was identified in Ontario. Only 9 of 120 zymodeme patterns identified were found to be pathogenic strains of E. histolytica. Pathogenic isolates were strongly correlated with clinical symptoms and seropositivity.
mesh_terms: D000818:Animals; D001955:British Columbia; D004748:Entamoeba histolytica / Q000201:enzymology*; D004749:Entamoebiasis / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D009864:Ontario
chemical_list: D007527:Isoenzymes