Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe.
Specific messenger RNA for glutamate dehydrogenase was partially purified from a calf liver polysomal poly(A)-mRNA fraction by sucrose density gradient centrifugation. Enzyme activity in the translational incubation mixture was detected by measuring NADH oxidation in the presence of alpha-ketoglutarate and ammonia as a decrease in absorbency 340-442 nm in a dual wavelength Aminco DW-2 spectrophotometer.
D000818:Animals; D002417:Cattle; D005969:Glutamate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D011061:Poly A / Q000302:isolation & purification; D011132:Polyribosomes / Q000201:enzymology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D013053:Spectrophotometry / Q000379:methods
D012333:RNA, Messenger; D011061:Poly A; D005969:Glutamate Dehydrogenase