Loss of one chromosome #13 during development of a polyposis tumor.
A cell panel from six different familial cancers, where both normal and tumor tissues were available, was examined for genotypic changes with polymorphic DNA probes. Seventeen probes were tested, representing chromosomes #1, #2, #5, #6, #13, #14, #17, and #19. One probe, p7F12 (D13S1, at 13q12-q14) revealed loss of heterozygosity in two tumors: an osteosarcoma from a patient with retinoblastoma that had been included as a control, and one polyposis tumor that had been established in nude mice from a duodenal carcinoma biopsy. Loss of heterozygosity was observed in the first passage of the mouse tumor. Chromosome analysis in later passages revealed loss of one whole chromosome #13 as the single consistent karyotypic change.
D011125:Adenomatous Polyposis Coli / Q000235:genetics; D000818:Animals; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13; D004273:DNA, Neoplasm / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009386:Neoplastic Syndromes, Hereditary / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014183:Transplantation, Heterologous
D004273:DNA, Neoplasm; D005819:Genetic Markers