[Subcellular localization of glutamine synthetase activity in carp muscle tissue and liver].
Subcellular distribution of the glutaminesynthetase activity (EC 6.3.1.2) is studied in muscle tissue and liver of carp. It is established that this activity is distributed by subcellular fractions analogously to the glutamatedehydrogenase activity--enzyme-marker activity of mitochondria. An additional evidence for the association of the two given enzymes is obtained during equilibrium density-gradient centrifugation of mitochondria on 30-60% gradient of sorbitol. Comparison of the obtained results with data from literature testifies to a coincidence of the subcellular localization of the processes of ammonia formation and binding into glutamine, thus confirming a view of the leading role of glutaminesynthetase in ammonia detoxication in carps.
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002347:Carps / Q000378:metabolism*; D003530:Cyprinidae / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000032:analysis; D005974:Glutamate-Ammonia Ligase / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
D000641:Ammonia; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase
Subkletochnaia lokalizatsiia glutaminsintetaznoĭ aktivnosti v myshechnoĭ tkani i pecheni karpa.