Determination of alpidem and its metabolites in human plasma by high-performance liquid chromatography and fluorimetric detection.
A high-performance liquid chromatographic method has been developed for the simultaneous determination of alpidem and its metabolites in human plasma. The method involved a single extraction of the parent drug and metabolites into diethyl ether from alkalinized plasma, evaporation of the organic solution and chromatography of the extracts on a C18 column coupled to a fluorimetric detector. An internal standard was used for the quantitative determination of the compounds. The method was selective for alpidem and three of its metabolites and has a limit of detection of less than 1 ng ml-1 for all the compounds. Since the chromatographic run took more than 20 min, the chromatographic process was fully automated and performed overnight.
D014151:Anti-Anxiety Agents / Q000097:blood* / Q000652:urine; D000927:Anticonvulsants / Q000097:blood* / Q000652:urine; D001711:Biotransformation; D006801:Humans; D007093:Imidazoles / Q000097:blood* / Q000652:urine; D007202:Indicators and Reagents; D007700:Kinetics; D011725:Pyridines / Q000097:blood* / Q000652:urine; D013050:Spectrometry, Fluorescence
D014151:Anti-Anxiety Agents; D000927:Anticonvulsants; D007093:Imidazoles; D007202:Indicators and Reagents; D011725:Pyridines; C052036:alpidem