Influence of adrenergic agonists and antagonists on lymphokine secretion in vitro.
Spleen cells of mice and lymph node lymphocytes of rats were cultured with epinephrine, norepinephrine and alpha- and beta-adrenergic drugs to determine effects on lymphokine secretion. After an incubation of 4.5 h it was found that the naturally occurring catecholamines and adrenergic drugs (isoprenaline, orciprenaline, terbutaline, norfenephrine, phenylephrine, clonidine, dobutamine) could induce the secretion of charge-changing lymphokines and lymphokines that stimulate the migration of lymphocytes. The beta-blocker propranolol inhibited the lymphokine secretion evoked by epinephrine and by beta-adrenergic agonists, whereas the alpha-blocker phentolamine antagonized the effects elicited by alpha-adrenergic drugs. Phenylethylamine also induced a significant secretion of lymphokines. Two non-phenylethylamines had no effect. The stimulatory capacity of adrenergic agonists, the inhibitory activity of the appropriate antagonists and the comparison with two non-phenylethylamines support the hypothesis on specific influence of catecholamines on the lymphocyte function.
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010627:Phenethylamines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000187:drug effects / Q000378:metabolism
D000317:Adrenergic alpha-Antagonists; D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D008222:Lymphokines; D010627:Phenethylamines