title: Evidence against VIP as the inhibitory transmitter in non-adrenergic, non-cholinergic nerves supplying the longitudinal muscle of the mouse colon.
abstract: Vasoactive intestinal peptide (VIP) had two types of effects on the longitudinal muscle of the mouse distal colon. At low concentrations (10(-8) M) VIP induced a contraction which seemed to be related to the production of prostaglandins as it was abolished after preincubation with indomethacin (10(-6) M). At higher concentrations (3 X 10(-8) and 10(-7) M) VIP induced relaxations which developed slowly and were related to stimulation of the adenylate cyclase activity of the smooth muscle cells. There is no evidence that VIP is the non-adrenergic, non-cholinergic transmitter released by electrical stimulation in this preparation and responsible for rapid relaxation of the smooth muscle.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D003106:Colon / Q000187:drug effects / Q000201:enzymology / Q000294:innervation*; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology; D009433:Neural Inhibition; D018377:Neurotransmitter Agents; D014660:Vasoactive Intestinal Peptide / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D018377:Neurotransmitter Agents; D014660:Vasoactive Intestinal Peptide; D000262:Adenylyl Cyclases; D007213:Indomethacin