Dynorphin A immunoreactivity in human cerebrospinal fluid.
Dynorphin A immunoreactivity in human cerebrospinal fluid has been characterized. Large quantities of the fluid were fractionated by molecular sieving on a Sephadex G-50 column and analyzed by radioimmunoassay. Active fractions were further analyzed by means of HPLC and an enzyme radioimmunoassay procedure for identification of Leu-enkephalin-Arg6 sequences. Several dynorphin A-active components of varying sizes were identified. However, most immunoreactive material derived from species of higher molecular weight (Mr 3000 and 5000) than authentic dynorphin A (Mr 2000). The Leu-enkephalin core was found to reside in both these large structures.
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins / Q000134:cerebrospinal fluid*; D006801:Humans; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D011863:Radioimmunoassay
D004399:Dynorphins