[Biochemical characteristics of a muscle perfusate and its effects on mediator release in the neuromuscular synapse].
Histidine-containing compounds (HCC) were found, using diazoreaction and reaction with diethylpyrocarbonate, a specific histidine reagent, in the perfusate of the frog hindlimb vasculature. After gel filtration on sephadex G-25 about 80% of HCC were eluted with a fraction with molecular mass less than 5000 kD. The studies of the perfusate influence on the characteristics of quantum secretion of transmitter in the preparation of frog cutaneopectoral muscle have shown that the perfusate increased the quantum content of end-plate potentials (EPP) due to increasing binominal parameter and decreasing frequency of miniature EPP. The characteristics of presynaptic action of the perfusate were similar to those of exogenous histidine.
D000818:Animals; D009132:Muscles / Q000098:blood supply / Q000502:physiology*; D009469:Neuromuscular Junction / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010477:Perfusion; D011898:Ranidae
D018377:Neurotransmitter Agents
Nekotorye biokhimicheskie kharakteristiki myshechnogo perfuzata i ego vliianie na osvobozhdenie mediatora v nervno-myshechnom sinapse.