Effects of amino-acid composition of the medium and addition of fatty-acid derivatives on the induction of lipogenic enzymes in cultured hepatocytes.
Effects of essential and non-essential amino acids on induction of lipogenic enzymes were investigated in cultured rat hepatocytes. Glucose-6-phosphate dehydrogenase was markedly induced by the addition of essential amino acids alone to the cultured medium, but was not induced by non-essential amino acids. Fatty-acid synthetase was also markedly induced by a combination of both amino-acid types (more than by either type of amino acid alone). However, acetyl-CoA carboxylase and malic enzyme were slightly induced by the addition of essential and/or non-essential amino acids. When various kinds of fatty acids were individually added to the medium, the lipid-dependent decreases in lipogenic enzyme inductions were in the following order: 18:2 greater than 20:4 greater than 18:1 greater than 16:0. When either linoleic acid, linoleoyl-CoA or trilinolein was added to the medium, linoleic acid was more effective as an inhibitor of the induction, without impairing the viability of cells.
D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis; D000214:Acyl Coenzyme A / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis*; D004790:Enzyme Induction; D064429:Fatty Acid Synthases / Q000096:biosynthesis; D005227:Fatty Acids / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D008099:Liver / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000096:biosynthesis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000494:pharmacology
D000214:Acyl Coenzyme A; D000596:Amino Acids; D003470:Culture Media; D005227:Fatty Acids; D014280:Triglycerides; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase