Aging: effect on ex-vivo benzodiazepine binding after a diazepam injection.
Ex vivo [3H]flunitrazepam receptor occupation was determined in the brain of young, mature and old male Fischer 344 rats after a single intravenous injection of a low dose of diazepam. The two benzodiazepine receptor subtypes or conformations (BZ1 and BZ2) were differentiated by the displacement of [3H]flunitrazepam specific binding with the triazolopyridazine, CL 218,872. The acute diazepam injection decreased ex vivo [3H]flunitrazepam binding in only the senescent rats. The [3H]flunitrazepam binding at both the BZ1 and BZ2 receptor or receptor conformation was significantly reduced in the old rats.
D000375:Aging; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D001667:Binding, Competitive; D001921:Brain / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D003975:Diazepam / Q000494:pharmacology*; D005445:Flunitrazepam / Q000378:metabolism; D007700:Kinetics; D008297:Male; D011724:Pyridazines / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*
D014151:Anti-Anxiety Agents; D011724:Pyridazines; D011963:Receptors, GABA-A; D005445:Flunitrazepam; C021604:CL 218872; D003975:Diazepam