title: Fimbrial phase variation in Escherichia coli: dependence on integration host factor and homologies with other site-specific recombinases.
abstract: Expression of fimA, the structural gene for type 1 fimbriae of Escherichia coli, is phase variable. Significant homologies were identified between the recombinases which control fimbrial phase variation, FimB and FimE, and the integrase class of site-specific recombinases. Normal expression of fimA was shown to require the integration host factor (IHF). Mutations in either the himA-or the himD (hip) gene, which encode the alpha and beta subunits of IHF, respectively, prevented phase variation and locked expression of fimA in either the "on" or "off" phase. In addition, both himA and himD lesions caused a sevenfold reduction in expression of a phi(fimA-lacZ) operon fusion in strains in which fimA was locked in the on phase. Thus, IHF plays a dual role in controlling fimA expression: it is required both for inversion of the fimA control region and for efficient expression from the fimA promoter. A mechanism by which IHF may exert control over fimA expression is discussed.
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D010861:Fimbriae, Bacterial; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D034982:Integration Host Factors; D009154:Mutation; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D034982:Integration Host Factors; D004254:DNA Nucleotidyltransferases