title: Sequence comparisons of complementary DNAs encoding aequorin isotypes.
abstract: Aequorin is the Ca2+-activated photoprotein which participates in the bioluminescence from the circumoral ring of the hydromedusa Aequorea victoria. The nucleotide sequences of five aequorin cDNAs have been compared and shown to code for three aequorin isoforms. The cDNA AEQ1 contains the entire protein coding region of 196 amino acids. The other four cDNAs contain only 70-90% of the coding region and apparently code for at least two other isoforms whose amino acid sequences differ significantly from that encoded by AEQ1. The nucleotide sequences coding for the three isotypes differ at a minimum of 54 positions out of a total of 588 nucleotides necessary to code for apoaequorin. Of these nucleotide differences, 24 account for 23 amino acid replacements, substantiating the microheterogeneity observed during sequencing of purified native aequorin [Charbonneau, H., Walsh, K.A., McCann, R.O., Prendergast, F.G., Cormier, M.J., & Vanaman, T.C. (1985) Biochemistry 24, 6762-6771]. Comparison of the deduced cDNA translations with the native protein sequences suggests the loss of seven residues from the amino terminus during purification of aequorin from Aequorea. Aequorin rapidly extracted from the jellyfish using conditions to minimize proteolysis is shown to have a larger molecular weight than that of purified native aequorin. Escherichia coli expressed aequorin encoded by AEQ1 is shown to have the same molecular weight and isoelectric point as those of one of the isotypes rapidly extracted from Aequorea.
mesh_terms: D000331:Aequorin / Q000235:genetics*; D001059:Apoproteins / Q000235:genetics; D001483:Base Sequence; D003063:Cnidaria / Q000235:genetics; D004247:DNA / Q000235:genetics; D004926:Escherichia coli; D007526:Isoelectric Point; D008164:Luminescent Proteins / Q000235:genetics*; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001059:Apoproteins; D008164:Luminescent Proteins; D000331:Aequorin; D004247:DNA