Transamidating activities of factor XIIIa and of transglutaminases, measured by an ELISA procedure.
The dansyl hapten in dansylcadaverine offers unique possibilities for measuring the incorporation of the monoamine into proteins (e.g. N,N'-dimethylcasein) by transamidating enzymes such as factor XIIIa and the transglutaminases. The protein-bound dansylcadaverine was assayed by an ELISA procedure based on a monoclonal antibody to the dansyl moiety.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D002103:Cadaverine / Q000031:analogs & derivatives / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000201:enzymology; D005176:Factor XIII / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D008099:Liver / Q000201:enzymology; D013379:Substrate Specificity; D011503:Transglutaminases / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005176:Factor XIII; D000217:Acyltransferases; D011503:Transglutaminases; C008542:monodansylcadaverine; D002103:Cadaverine