Immunocytochemical localizations of cytosolic and mitochondrial glutamic oxaloacetic transaminase isozymes in rat primary sensory neurons as a marker for the glutamate neuronal system.
The localization of cytosolic (s-) and mitochondrial (m-) glutamic oxaloacetic transaminase (GOT) was examined in the rat trigeminal, jugular and dorsal root ganglia by means of an indirect immunofluorescence method using antibodies specific for s- and m-GOT. Staining of s-GOT-like immunoreactivity was seen in giant, large, medium and small cells in these ganglia. On the other hand, m-GOT-like immunoreactivity was not seen in them. The distribution of GOT suggests that glutamate may be a transmitter released from primary sensory neurons.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D005724:Ganglia / Q000201:enzymology*; D005727:Ganglia, Spinal / Q000201:enzymology; D005971:Glutamates / Q000502:physiology*; D018698:Glutamic Acid; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D009475:Neurons, Afferent / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D009435:Synaptic Transmission; D012668:Trigeminal Ganglion / Q000201:enzymology
D005971:Glutamates; D007527:Isoenzymes; D018698:Glutamic Acid; D001219:Aspartate Aminotransferases