Immunocytochemical and ultrastructural characterization of human T-lymphotropic virus type I (HTLV-I)-producing rabbit lymphoid cell lines.
Fine structural and immunocytochemical characterization of rabbit lymphoid cell lines transformed by human T-lymphotropic virus type I (HTLV-I) was carried out. All nine cell lines tested were reactive with anti-HTLV-I-positive human, monkey, and rabbit sera and monoclonal antibody to HTLV-Ip 19, but not with anti-HTLV-I-negative sera and monoclonal antibodies to human Ia and pan-T antigens. All cell lines were strongly positive for monoclonal antibodies to rabbit Ia and pan-T antigens. Ultrastructurally, each cell line contained C-type virus particles in varying numbers in the extracellular space. These particles showed replication patterns similar to those in HTLV-I or simian T-lymphotropic virus type I (STLV-I)-producing human or monkey cells. In addition, anti-HTLV-I-positive rabbit serum gave positive immunoreactivity to HTLV-I or STLV-I by indirect immunoferritin method. These results indicate that the ultramorphology and replication patterns of HTLV-I in rabbit cell lines are indistinguishable from those of HTLV-I in human and monkey cell lines, HTLV-I in rabbit cells shares the common surface antigenic determinants with HTLV-I or STLV-I in human or monkey cells, and that these cells are definitely rabbit T cells bearing their own Ia antigens.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D017977:Deltaretrovirus / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D015480:Deltaretrovirus Antibodies; D000882:Haplorhini; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D009940:Organoids / Q000648:ultrastructure; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D014779:Virus Replication
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral; D015480:Deltaretrovirus Antibodies; D000949:Histocompatibility Antigens Class II