Simple non-invasive method to obtain DNA for gene analysis.
A technique is described which demonstrates that sufficient human DNA for direct gene analysis can be isolated from buccal epithelial cells obtained by mouthwash. This procedure is much simpler and cheaper than existing methods and, combined with DNA amplification by polymerase chain reaction, should allow community screening for carrier status for single gene defects such as cystic fibrosis.
D002610:Cheek; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D004259:DNA-Directed DNA Polymerase; D005820:Genetic Testing / Q000379:methods; D006197:Hair / Q000032:analysis; D006239:Haplotypes; D006801:Humans; D009061:Mouth Mucosa / Q000032:analysis; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic
D004247:DNA; D004259:DNA-Directed DNA Polymerase