Pre- and postweaning development of drug-metabolizing enzyme activities in small intestine and liver of rats.
The postnatal development of NADPH-cytochrome c reductase, UDP-glucuronosyltransferase, selenium-dependent glutathione peroxidase, glutathione-S-transferase activities, total non-protein sulfhydryls, and cytochrome P-450 contents was compared in small intestine and liver of male Wistar rats from 18 to 60 days of life. In the intestine, main age-related changes affected conjugation enzymes which were increased rapidly by a factor of 4 during the weaning time and remained unchanged thereafter. Development in the rat was accompanied by changes in cytochrome P-450 content and NADPH-cytochrome c reductase activity only in the liver. In both organs, glutathione concentration and selenium-dependent glutathione peroxidase activity were not significantly modified between 18 and 60 days. In the liver, we observed discontinuities in the developmental pattern of UDP-glucuronosyltransferase, and the ontogenesis of glutathione-S-transferase activities differed according to the substrate metabolized.
D000375:Aging / Q000378:metabolism; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007421:Intestine, Small / Q000201:enzymology* / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013438:Sulfhydryl Compounds / Q000378:metabolism
D004364:Pharmaceutical Preparations; D013438:Sulfhydryl Compounds; D005979:Glutathione Peroxidase; D009251:NADPH-Ferrihemoprotein Reductase; D014453:Glucuronosyltransferase