On the presence of opioid receptors in guinea-pig ventricular tissue.
The cardiac response to sympathetic nerve stimulation, induced by trains of field pulses, was studied in isolated guinea-pig ventricular strips. Dynorphin-(1-13) and [D-Ala2, D-Leu5]enkephalinamide, but not morphine, reduced, in a dose-dependent manner, the cardiac sympathetic response. The effect of the two opioid peptides was antagonized by naloxone. The opioid agonists did not affect the response to exogenous noradrenaline. Neither naloxone nor a mixture of peptidase inhibitors modified the cardiac response to sympathetic nerve stimulation.
D000818:Animals; D004399:Dynorphins / Q000494:pharmacology; D004558:Electric Stimulation; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives*; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology; D006892:Hydroxydopamines / Q000494:pharmacology; D066298:In Vitro Techniques; D009206:Myocardium / Q000032:analysis*; D009270:Naloxone / Q000494:pharmacology; D009294:Narcotics / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D016627:Oxidopamine; D010446:Peptide Fragments / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D011957:Receptors, Opioid / Q000032:analysis* / Q000187:drug effects; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology
D006892:Hydroxydopamines; D009294:Narcotics; D010446:Peptide Fragments; D011480:Protease Inhibitors; D011957:Receptors, Opioid; D009270:Naloxone; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; C030279:enkephalinamide-Leu, Ala(2)-; C026843:dynorphin (1-13); D004399:Dynorphins; D016627:Oxidopamine; D009638:Norepinephrine