Transcriptional induction of cellular gene expression during lytic infection with herpes simplex virus.
Herpes simplex virus Type 2 causes a severe repression of host cell biosynthesis at a number of levels. We show that despite this, non-viral cDNA clones derived from cellular RNA species which accumulate to high levels after infection can be isolated using differential screening techniques. By using nuclear run-off assays, we have shown that this RNA accumulation is mediated by transcriptional induction of the corresponding cellular genes.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005796:Genes; D008647:Mesocricetus / Q000235:genetics; D011061:Poly A / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D018139:Simplexvirus / Q000502:physiology*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011061:Poly A; D004247:DNA