Prevalence of K88, K99, and 987P pili of Escherichia coli in neonatal pigs with enteric colibacillosis.
One hundred nineteen live neonatal pigs with diarrhea less than or equal to 2 weeks old were euthanatized, and frozen sections of their ilea were submitted to an indirect fluorescent antibody technique to identify K88, K99, and 987P pili (also referred to as F-4, F-5, and F-6 pili, respectively) in Escherichia coli. Ten-centimeter ileal sections were used to determine numbers of lactose-fermenting bacteria. Of 52 pigs in which E coli pili were found, 14 had K88 (27%), 23 had K99 (44%), 13 had 987P (25%), and 2 had K88 and K99 simultaneously (4%). Numbers of lactose-fermenting bacteria were significantly (P less than or equal to 0.05) higher in pigs with piliated E coli than in pigs without piliated E coli. Results of this study indicated that piliated E coli are a major cause of enteric disease in neonatal swine in Michigan, and that in pigs less than or equal to 2 weeks of age, K99 was the most frequently encountered pilus antigen.
D000818:Animals; D000831:Animals, Newborn; D000942:Antigens, Bacterial / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001427:Bacterial Toxins; D003332:Coronaviridae / Q000302:isolation & purification; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000276:immunology / Q000648:ultrastructure*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007082:Ileum / Q000382:microbiology; D012401:Rotavirus / Q000302:isolation & purification; D012475:Salmonella / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; C031429:K99 antigen; D036781:Fimbriae Proteins