Stimulation of soluble guanylate cyclase by endothelium-derived relaxing factor from cultured endothelial cells.
Bovine endothelial cells, grown on microcarrier beads and superfused with a saline solution, were stimulated with thimerosal or bradykinin to release endothelium-derived relaxing factor (EDRF). EDRF activity in the effluent was assayed in endothelium-denuded rabbit aorta. The stimulation of purified soluble guanylate cyclase in test tubes by the EDRF-containing effluent amounted to 90-fold of basal activity and its time course correlated with that of the dilator response of the aorta. After preincubation of endothelial cells with gossypol the EDRF-induced dilator response as well as the stimulation of guanylate cyclase was suppressed.
D000818:Animals; D001013:Aorta, Thoracic / Q000201:enzymology; D001920:Bradykinin / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004727:Endothelium / Q000502:physiology; D004789:Enzyme Activation / Q000187:drug effects; D006072:Gossypol / Q000494:pharmacology; D006162:Guanylate Cyclase / Q000378:metabolism*; D009569:Nitric Oxide; D013849:Thimerosal / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology*
D014665:Vasodilator Agents; D013849:Thimerosal; D009569:Nitric Oxide; D006162:Guanylate Cyclase; D006072:Gossypol; D001920:Bradykinin