Immune serum from both susceptible and resistant strains of mice increases phagocytosis of Leishmania mexicana amazonensis by macrophages.
Immune sera obtained from either BALB/c mice (susceptible) at 7 weeks, or C57BL/6 mice (resistant), at 7 weeks after infection with L. m. amazonensis, were effective in increasing internalization of homologous promastigotes into starch-induced peritoneal macrophages (from both mouse strains). Both the internalization enhancing effect and the levels of anti-leishmanial antibody (ELISA) were removed from sera by absorption with heat-killed promastigotes. Sera at 1/200 dilution obtained from either mouse strain at 2 weeks after infection did not enhance parasite internalization into macrophages. The factors leading to susceptibility or resistance during leishmaniasis do not appear to be related to differences in antibody-mediated opsonic activity.
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D004198:Disease Susceptibility; D007106:Immune Sera / Q000276:immunology; D007113:Immunity, Innate; D007116:Immunization, Passive; D007894:Leishmania mexicana / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009895:Opsonin Proteins / Q000276:immunology; D010587:Phagocytosis
D000913:Antibodies, Protozoan; D007106:Immune Sera; D009895:Opsonin Proteins