Supplementation of Ham's F10 culture medium with three different sera in the culturing of baboon oocytes.
1. Ten female baboons (Papio ursinus) were stimulated for a total of 20 cycles with 3 ovulation induction agents. 2. Oocytes obtained were randomly allocated to Ham's F10 culture medium supplemented with human fetal cord serum, primate serum or commercial fetal bovine serum respectively. 3. Fertilization occurred (38.1-45.5%) in all 3 supplements, but cleavage and embryo development was more successful in culture medium supplemented with fetal bovine serum. 4. Eight embryos were cultured and 6 (75%) of these were cultured in fetal bovine serum supplemented medium.
D000818:Animals; D002478:Cells, Cultured; D002996:Clomiphene / Q000494:pharmacology; D003470:Culture Media; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D009865:Oocytes / Q000166:cytology*; D010215:Papio / Q000502:physiology*
D003470:Culture Media; D002996:Clomiphene