Messenger RNA from insect nervous tissue induces expression of neuronal acetylcholine receptors in Xenopus oocytes.
mRNA isolated from the nervous tissue of young insects microinjected in Xenopus oocytes induced the expression of alpha-toxin binding sites which were inserted into the surface membrane. Immunoprecipitation experiments revealed that the coded receptor polypeptides were processed to the authentic size, and ion flux studies demonstrated that functional nicotinic acetylcholine receptors were produced and inserted into the oocyte membrane.
D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D011232:Chemical Precipitation; D005260:Female; D006110:Grasshoppers / Q000235:genetics*; D007158:Immunologic Techniques; D009417:Nerve Tissue / Q000032:analysis; D009865:Oocytes / Q000378:metabolism; D011061:Poly A / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D011868:Radioisotopes; D011978:Receptors, Nicotinic / Q000096:biosynthesis / Q000235:genetics*; D012413:Rubidium; D014982:Xenopus laevis
D002038:Bungarotoxins; D012333:RNA, Messenger; D011868:Radioisotopes; D011978:Receptors, Nicotinic; D011061:Poly A; D012413:Rubidium