title: Immunohistochemical visualization of glutamate- and aspartate-containing nerve terminal pools in the rat limbic structures.
abstract: Antisera raised against glutamate or aspartate bound to bovine serum albumin were purified by affinity chromatography, and their specificities were verified by immunoblotting and by enzyme-linked immunosorbent assay. Immunohistochemical investigation using materials perfusion-fixed after long flushing demonstrated distinct laminar terminals with glutamate- or aspartate-like immunoreactivity throughout the limbic structures. This technique may offer a valuable tool for revealing the distribution of glutamatergic or aspartatergic nerve terminals.
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000276:immunology / Q000378:metabolism*; D005971:Glutamates / Q000276:immunology / Q000378:metabolism*; D018698:Glutamic Acid; D006624:Hippocampus / Q000378:metabolism; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008032:Limbic System / Q000378:metabolism*; D008297:Male; D009830:Olfactory Bulb / Q000378:metabolism; D051381:Rats; D013569:Synapses / Q000378:metabolism
chemical_list: D005971:Glutamates; D007106:Immune Sera; D001224:Aspartic Acid; D018698:Glutamic Acid