A mutation of the c subunit of the Escherichia coli proton-translocating ATPase that suppresses the effects of a mutant b subunit.
A mutation of the b subunit of the Escherichia coli proton-translocating ATPase and mutations in the gene for the a subunit that suppress its effects have been previously described (Kumamoto, C., and Simoni, R. D. (1986) J. Biol. Chem. 261, 10037-10042). In this paper, we describe the characterization of a new mutation that partially suppresses the effects of the original b mutation. The new suppressor mutation causes the substitution of serine for alanine at position 62 of the c subunit. Biochemical studies of double mutants, carrying both b and c mutations, demonstrate that the c mutation partially restores the function of the enzyme complex.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D025243:Bacterial Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D009154:Mutation; D009243:NAD / Q000494:pharmacology; D010957:Plasmids; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D013489:Suppression, Genetic; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D009243:NAD; D004024:Dicyclohexylcarbodiimide; D000255:Adenosine Triphosphate; C523987:ATP synthase subunit c, E coli; D025243:Bacterial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases