Three industrial solvents investigated for tumor promoting activity in the rat liver.
Dioxane, perchloroethylene, and trichloroethylene were investigated as rat liver altered foci promoters in an initiation/promotion protocol. Animals were initiated with diethylnitrosamine, 30 mg/kg, injected i.p. 24 h after 2/3 partial hepatectomy. The chemical under study was administered by gavage once a day, 5 times a week for 7 weeks. Ten days after the last administration the animals were killed. Liver sections were stained for gamma-glutamyl-transpeptidase (GGT) and the number and total volume of GGT-positive foci was studied. Dioxane (1000 mg/kg) significantly increased the number and total volume of foci while a marginal effect was noted for the high dose of trichloroethylene (1100 mg/kg). A high dose of perchloroethylene (1100 mg/kg) had no effect.
D000284:Administration, Oral; D000818:Animals; D002273:Carcinogens; D004052:Diethylnitrosamine / Q000633:toxicity; D004146:Dioxanes / Q000633:toxicity*; D004147:Dioxins / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013750:Tetrachloroethylene / Q000633:toxicity*; D014241:Trichloroethylene / Q000633:toxicity*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D002273:Carcinogens; D004146:Dioxanes; D004147:Dioxins; D014241:Trichloroethylene; D004052:Diethylnitrosamine; D005723:gamma-Glutamyltransferase; C025223:1,4-dioxane; D013750:Tetrachloroethylene