Contact smear cytologic preparations of organ culture explant tissue.
The effects of cytologic sampling by contact-smear on the viability and morphology of epithelial tissues maintained as explants in organ culture were studied. Tracheal and pancreatic tissues from Syrian golden hamsters were maintained for 30 days in culture, and sampled cytologically at weekly intervals. Analysis of sequential Papanicolaou-stained smears demonstrates that explant tissues can be evaluated cytologically over time in vitro without apparent effects on either the tissue viability, histochemistry or morphology. Microscopic examination further reveals that cellular samples retain diagnostically significant cytomorphologic features of the in vivo tissues. These observations indicate that this technique is useful for understanding the long-term response to carcinogens of epithelial tissue maintained in explant organ culture.
D000818:Animals; D006224:Cricetinae; D003584:Cytological Techniques; D006651:Histocytochemistry; D009924:Organ Culture Techniques; D010179:Pancreas / Q000166:cytology / Q000378:metabolism; D013048:Specimen Handling / Q000379:methods*; D014132:Trachea / Q000166:cytology / Q000378:metabolism