A vasoactive intestinal polypeptide system in retinal cell cultures: immunocytochemistry and physiology.
The purpose of this study was to search for vasoactive intestinal polypeptide (VIP)-like immunoreactivity and VIP-mediated effects in cultures containing cells from the mammalian retina. VIP-like immunoreactivity was detected by indirect immunocytochemistry within 6 days after plating dissociated retinal cells from embryonic day-19 rats. In electrophysiological experiments, VIP was found to facilitate evoked transmission at cholinergic synapses formed by retinal neurons in culture.
D000109:Acetylcholine / Q000378:metabolism; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006651:Histocytochemistry; D009132:Muscles / Q000166:cytology / Q000502:physiology; D051381:Rats; D012160:Retina / Q000032:analysis* / Q000166:cytology / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000032:analysis* / Q000502:physiology
D005971:Glutamates; D014660:Vasoactive Intestinal Peptide; D018698:Glutamic Acid; D000109:Acetylcholine