Attempt to detect recombination between B-F and B-L genes within the chicken B complex by serological typing, in vitro MLR, and RFLP analyses.
In search for recombinants within the chicken major histocompatibility B complex, 1155 animals from crosses between the congenic lines CB (B12) and CC (B4) were tested with alloantibodies and monoclonal antibodies for the B-F (class I), B-L (class II), and B-G (class IV) antigens and by mixed lymphocyte reaction. The absence of detectable recombination was confirmed by restriction fragment length polymorphism analysis with B-L beta and B-F probes. Together with previous reports, this indicates that the distance between the B-F and B-L loci is below 0.01 centimorgan.
D000818:Animals; D001788:Blood Grouping and Crossmatching; D002645:Chickens; D015342:DNA Probes; D005260:Female; D005802:Genes, MHC Class II; D008040:Genetic Linkage; D005838:Genotype; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000302:isolation & purification; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic
D015342:DNA Probes; D000949:Histocompatibility Antigens Class II; C041136:Ia-like antigens, avian