Modulation of amino acid and 2-oxo acid pools in Trichomonas vaginalis by aspartate aminotransferase inhibitors.
The amino acid pool sizes of Trichomonas vaginalis are reported. Alanine, glutamic acid, proline and leucine account for 72% of the measured amino acids. Growth of T. vaginalis was unaffected by gostatin, an irreversible inhibitor of aspartate aminotransferase, when the enzyme activity within the cell had been completely inhibited and a specific elevation of the aspartate pool had occurred. In media lacking aspartate and glutamate, the amino acid substrates of the aspartate aminotransferase reaction, gostatin caused a larger increase in the aspartate pool. During incubation of cells with or without gostatin, aspartate and glutamate were produced in the medium, presumably by proteolysis of medium proteins. Hence any requirement for the aspartate aminotransferase reaction might have been bypassed. Glutamate-gamma-hydroxamate and aminooxyacetate inhibited growth of T. vaginalis but caused large changes in the pool-sizes of aspartate, glutamate, pyruvate plus oxaloacetate and 2-oxoglutarate, suggesting a more general interference with amino acid metabolism.
D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000600:Amino Acids, Dicarboxylic / Q000494:pharmacology*; D000625:Aminooxyacetic Acid / Q000494:pharmacology; D000818:Animals; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors*; D003470:Culture Media; D005971:Glutamates / Q000378:metabolism / Q000494:pharmacology; D018698:Glutamic Acid; D006877:Hydroxamic Acids / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D014246:Trichomonas vaginalis / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
D000596:Amino Acids; D000600:Amino Acids, Dicarboxylic; D003470:Culture Media; D005971:Glutamates; D006877:Hydroxamic Acids; D000625:Aminooxyacetic Acid; C050614:glutamate-gamma-hydroxamic acid; D018698:Glutamic Acid; C042220:gostatin; D011392:Proline; D001219:Aspartate Aminotransferases; D007930:Leucine; D000409:Alanine