[Effect of phosphaden and unithiol on the cyclase system of the small intestine mucosa in rabbits in experimental salmonellosis].
The study was made on 59 chinchilla rabbits. S. typhimurium 1847 live culture was introduced into the lumen of an isolated loop of the thin intestine. The activity of adenylate cyclase (AC), guanylate cyclase (GC), the levels of cyclic adenosine 3,5-monophosphate (cAMP), cyclic guanosine 3,5-monophosphate (cGMP), the activity of cAMP- and cGMP-phosphodiesterases were determined in the mucous membrane of the ligated part of the intestine. Considerable fluid accumulation in the loop, activation of AC and cGMP-phosphodiesterase, a rise in the level of cAMP and a drop in the level of cGMP in the mucosa of the ligated part of the intestine were registered. In one group of the animals phosphadene and in the other group unitiol were introduced into the infected intestinal loop; as a result, a decrease in the accumulation of fluid in the loop, on the average, by 40% and a tendency to an increase in the level of cAMP and a drop in the level of cGMP in the mucous membrane of the ligated part of the intestine were observed. Changes in the level of cGMP play, seemingly, a more important role in the development of diarrhea in salmonellosis.
D000249:Adenosine Monophosphate / Q000627:therapeutic use*; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D003967:Diarrhea / Q000188:drug therapy / Q000201:enzymology; D004112:Dimercaprol / Q000031:analogs & derivatives*; D004353:Drug Evaluation, Preclinical; D006162:Guanylate Cyclase / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000201:enzymology; D007421:Intestine, Small / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D011817:Rabbits; D012481:Salmonella Infections, Animal / Q000188:drug therapy* / Q000201:enzymology; D012486:Salmonella typhimurium; D014494:Unithiol / Q000627:therapeutic use*
D004112:Dimercaprol; D014494:Unithiol; D000249:Adenosine Monophosphate; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase; D006152:Cyclic GMP
Vliianie fosfadena i unitiola na tsiklaznuiu sistemu slizistoĭ tonkoĭ kishki krolika pri éksperimental'nom sal'monelleze.