title: High sensitivity assay for the alpha 2 antagonist 6-chloro-3-methyl-2,3,4,5-tetrahydro-1H-3-benzazepine and its desmethyl metabolite in plasma using gas chromatography mass spectrometry with ammonia/CCl4 chemical ionization.
abstract: A rapid and highly sensitive assay for quantifying the alpha 2-adrenoceptor antagonist SK&F 86466 and a desmethyl metabolite has been developed, using capillary column gas chromatography/chemical ionization mass spectrometry. This assay uses deuterium-labeled analogs of the analytes as internal standards, and has a lower limit of quantification of 100 pg ml-1. A novel reagent gas, consisting of carbon tetrachloride in anhydrous ammonia, was used to achieve maximal sensitivity.
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000097:blood*; D000641:Ammonia; D001552:Benzazepines / Q000097:blood*; D002251:Carbon Tetrachloride; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D008832:Microchemistry
chemical_list: D000317:Adrenergic alpha-Antagonists; D001552:Benzazepines; D007202:Indicators and Reagents; C051051:SK&F 101055; D000641:Ammonia; D002251:Carbon Tetrachloride; C039139:benalfocin