title: Characterization of a 280-kD protein restricted to the coated pits of the renal brush border and the epithelial cells of the yolk sac. Teratogenic effect of the specific monoclonal antibodies.
abstract: Intermicrovillar areas and apical vesicles characterized by an extensive clathrin coat can be identified in some epithelial cell types. We describe a 280-kD protein, characteristic of these areas in the proximal tubule brush border and epithelial cells of the visceral yolk sac. When injected to 9-d pregnant rats, mAbs to the 280-kD protein regularly induced fetal resorption and/or malformations. Antibodies to a 330-kD protein that is also coated-pit-restricted had no effect. Our observations point to a key function for p280 and suggest that immunity to specific constituents of the receptor-mediated endocytotic system may be involved in the induction of fetal abnormalities.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000633:toxicity; D003034:Coated Pits, Cell-Membrane / Q000032:analysis*; D000013:Congenital Abnormalities / Q000209:etiology; D004705:Endocytosis; D011992:Endosomes / Q000032:analysis*; D004848:Epithelium / Q000032:analysis; D005260:Female; D005327:Fetal Resorption / Q000209:etiology; D007687:Kidney Tubules, Proximal / Q000032:analysis*; D008871:Microvilli / Q000032:analysis*; D011247:Pregnancy; D051381:Rats; D015017:Yolk Sac / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal