Functional reconstitution of an isolated sodium channel from bovine trachea.
An amiloride-sensitive Na+ channel from bovine trachea was isolated using an affinity gel and reconstituted into a planar lipid bilayer. This channel exhibited: 1. Fluctuations with long duration opening and closing times, weak voltage dependence, and a conductance of 6 pS. 2. Selectivity of at least 100-fold for Na+ over K+. 3. Saturates at a Na+ concentration of 90 mM. 4. Blocked by amiloride, 50% inhibition at 0.1 microM.
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology*; D014132:Trachea / Q000502:physiology*
D015222:Sodium Channels; D000584:Amiloride; D012964:Sodium; D011188:Potassium