title: Expression of multiple types of N-methyl Phe pili in Pseudomonas aeruginosa.
abstract: The nature of pili synthesized by Pseudomonas aeruginosa when plasmid-borne genes of homologous pilins from Bacteroides nodosus are introduced as thermoregulated expression systems has been ascertained. Expression of B. nodosus pili inhibited the production of indigenous P. aeruginosa pili, and an organism harbouring pilin genes from two strains of B. nodosus produced two serologically distinct populations of pili on each cell. Simultaneous production of both indigenous and foreign pili was achieved by partial induction of expression. Homogeneity in pilus structure suggests either that there is an exclusive specificity of interaction between identical pilin subunits in pilus assembly, or that each pilus is produced from the translation products of a single messenger RNA molecule, with translation and pilus assembly closely coupled.
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000378:metabolism* / Q000648:ultrastructure; D005796:Genes; D005798:Genes, Bacterial; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D001425:Bacterial Outer Membrane Proteins; D036781:Fimbriae Proteins