Synthesis of platelet activating factor by cholinephosphotransferase in developing fetal rabbit lung.
Developing fetal lung is a possible source of the platelet activating factor (PAF, 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) present in amniotic fluid of women in labor. We have assayed the microsomal activities of a specific enzyme for the de novo synthesis of PAF in developing fetal and neonatal rabbit lung, 1-alkyl-2-acetyl-glycerol-dependent dithiothreitol-insensitive cholinephosphotransferase. The specific activity of this enzyme increased from 0.92 to 3.60 nmol X min-1 X mg-1 protein between day 21 and day 31 of gestation. In constrast, during this same period the activity of the PAF-biosynthetic cholinephosphotransferase in developing rabbit kidney did not change significantly. The specific activity of the diacyl-glycerol-dependent, dithiothreitol-sensitive cholinephosphotransferase that catalyzes the final step in phosphatidylcholine biosynthesis was not altered during late gestation in either fetal lung or kidney. Previously, increased amounts of pulmonary PAF had been found during the latter stages of gestation (Hoffman, Truong and Johnston (1986) Biochim. Biophys. Acta 879, 88-96) and may be attributed to increased activity of the PAF biosynthetic enzymes found in this investigation. This elevated level of PAF in fetal lung may serve to facilitate breakdown of glycogen that provides, in part, the carbon and energy source for surfactant biosynthesis. In addition, PAF may be secreted in association with surfactant into amniotic fluid in which it may interact with amnion tissue and subsequently participate in the events associated with the initiation of parturition.
D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism / Q000502:physiology*; D004229:Dithiothreitol / Q000494:pharmacology; D005260:Female; D007668:Kidney / Q000196:embryology / Q000201:enzymology; D008168:Lung / Q000196:embryology / Q000201:enzymology*; D010770:Phosphotransferases / Q000502:physiology*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000378:metabolism; D011247:Pregnancy; D011817:Rabbits
C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D000101:Acetyl-CoA C-Acetyltransferase; D010770:Phosphotransferases; D002798:Diacylglycerol Cholinephosphotransferase; D004229:Dithiothreitol