An HPLC assay procedure of sensitivity and stability for measurement of acetylcholine and choline in neuronal tissue.
A method is presented for the sensitive and specific determination of acetylcholine and choline in neuronal tissue. The method is based on the separation of acetylcholine and choline by reversed-phase HPLC, passing the eluent into a post-column reactor containing choline oxidase and acetylcholinesterase covalently bound to vinyl sulphone bonded onto a hydroxyethyl methacrylate support, and electrochemical detection of the hydrogen peroxide formed. The limit of detection of the procedure is 1 pmol for acetylcholine and 500 fmol for choline. The excellent baseline stability of the method ensures the rapid and reliable processing of a large number of samples.
D000109:Acetylcholine / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002794:Choline / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D008297:Male; D009474:Neurons / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D002794:Choline; D000109:Acetylcholine