5-Aminosalicylic acid protects against ischemia/reperfusion-induced gastric bleeding in the rat.
The aim of the present study was to determine whether the split products of sulfasalazine, sulfapyridine, and 5-aminosalicylic acid can ameliorate ischemia/reperfusion-induced injury to the gastric mucosa. Gastric mucosal damage was assessed by measuring (a) 51Cr-labeled red blood cell leakage into the gastric lumen, (b) the area of gross mucosal lesions, and (c) the extent of histologically demonstrable mucosal damage. In rats treated with 5-aminosalicylic acid, but not in those treated with sulfapyridine, the leakage of 51Cr-labeled red blood cells and the area of gross mucosal lesions after ischemia/reperfusion were significantly reduced as compared with untreated (control) rats. Inasmuch as 5-aminosalicylic acid (the therapeutic moiety of sulfasalazine) has been reported to be a hydroxyl radical scavenger, we also assessed the effects of dimethylsulfoxide (another hydroxyl radical scavenger) on ischemia/reperfusion-induced gastric mucosal injury. In rats treated with dimethylsulfoxide, leakage of 51Cr-labeled red blood cells and the area of gross mucosal lesions after ischemia/reperfusion were significantly reduced as compared with control rats. The results of this study support the contention that ischemia/reperfusion-induced gastric bleeding involves the hydroxyl radical and indicate that 5-aminosalicylic acid significantly attenuates this vascular injury.
D000636:Aminosalicylic Acids / Q000627:therapeutic use*; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000627:therapeutic use; D006471:Gastrointestinal Hemorrhage / Q000209:etiology / Q000517:prevention & control*; D006878:Hydroxides; D017665:Hydroxyl Radical; D007511:Ischemia / Q000150:complications / Q000188:drug therapy*; D008297:Male; D019804:Mesalamine; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000098:blood supply*; D013272:Stomach Diseases / Q000209:etiology / Q000517:prevention & control*; D012460:Sulfasalazine / Q000627:therapeutic use
D000636:Aminosalicylic Acids; D006878:Hydroxides; D017665:Hydroxyl Radical; D012460:Sulfasalazine; D019804:Mesalamine; D004121:Dimethyl Sulfoxide