Removal of lipopolysaccharide from acellular Bordetella pertussis vaccine by detergent treatment.
Protective antigen was extracted from Bordetella pertussis cells with 1.0 M NaCl and precipitated with ammonium sulfate, 20-40% saturation (designated fraction 15A-1B). The protective antigen was purified further by detergent (Emulphogene BC720) treatment and adsorption to aluminum hydroxide gel (designated fraction 15A-108A). Compared with B. pertussis vaccine and fraction 15A-1B, fraction 15A-108A retained protective activity as assessed by the mouse protection test, but had reduced protein and markedly reduced endotoxin content. Fraction 15A-108A also had reduced leukocytosis-promoting, histamine sensitizing splenomegaly-inducing, and adjuvant activities. Emulphogene treatment provided a relatively simple method for removing endotoxin from a potential acellular B. pertussis vaccine.
D000276:Adjuvants, Immunologic / Q000502:physiology; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001886:Bordetella pertussis / Q000187:drug effects / Q000276:immunology; D002458:Cell Fractionation; D003902:Detergents / Q000494:pharmacology*; D006632:Histamine / Q000008:administration & dosage / Q000276:immunology; D007114:Immunization; D007964:Leukocytosis / Q000209:etiology; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size; D010567:Pertussis Vaccine / Q000032:analysis*; D013154:Spleen / Q000473:pathology; D013501:Surface-Active Agents / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D003902:Detergents; D008070:Lipopolysaccharides; D010567:Pertussis Vaccine; D013501:Surface-Active Agents; D006632:Histamine