Demonstration of specific E-type prostaglandin receptors using enriched preparations of canine parietal cells and [3H]misoprostol free acid.
High-affinity, E-type prostaglandin binding sites in enriched canine parietal cell preparations were identified with [3H] misoprostol free acid, a prostaglandin E1 analogue. Saturable, reversible, and highly stereospecific binding was identified, with approximately 8,000 binding sites per cell. Prostaglandin I and F bound weakly, and cimetidine and histamine did not bind. The results indicate that [3H] misoprostol free acid binds to E-type prostaglandin receptors, which suggests that the ulcer-healing inhibition of gastric acid secretion by misoprostol results from its interaction with a specific E-type prostaglandin receptor.
D000527:Alprostadil / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000378:metabolism*; D004285:Dogs; D016595:Misoprostol; D010295:Parietal Cells, Gastric / Q000032:analysis*; D011458:Prostaglandins E / Q000494:pharmacology; D011982:Receptors, Prostaglandin / Q000032:analysis* / Q000187:drug effects; D018078:Receptors, Prostaglandin E; D014316:Tritium
D000897:Anti-Ulcer Agents; D011458:Prostaglandins E; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D016595:Misoprostol; D014316:Tritium; D000527:Alprostadil