Identification of a restriction fragment length polymorphism involving the oncogene ETS-1 on chromosome 11q23.
Twenty four samples of DNA from 23 unrelated individuals were analyzed for the presence of a novel restriction fragment length polymorphism (RFLP) involving the proto-oncogene ETS-1 at an Xba I site. Four samples from unrelated individuals lacked an Xba I site, giving rise to a longer restriction fragment detectable by Southern analysis; two samples were from normal tissue, and two were from acute myelogenous leukemic blasts. Thus, no association could be found between the RFLP and disease among the individuals studied. Pedigree analysis of another cohort demonstrated Mendelian inheritance consistent with a somatic polymorphism. The practical applications of RFLP analysis in clinical and research settings, and the usefulness of this Xba I RFLP in the study of hematologic malignancies because of its location in 11q23, are discussed.
D000208:Acute Disease; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000648:ultrastructure*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007938:Leukemia / Q000235:genetics; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D000090063:Proto-Oncogene Mas; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets; D011519:Proto-Oncogenes; D014157:Transcription Factors; D014178:Translocation, Genetic
C494041:ETS1 protein, human; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors; D003101:Collodion