DR beta Taq I restriction fragment length polymorphism (RFLP) associated with insulin dependent diabetes mellitus (IDDM).
DNA from insulin-dependent diabetes mellitus patients (IDDM) and healthy control individuals was evaluated using Southern blotting to determine if a disease-associated restriction fragment length polymorphism could be found. Using a DR beta-cDNA probe hybridized to Taq I digested genomic DNA, a 3.7 kb fragment was found in all IDDM patients examined (n = 33). Although a high percentage (47 per cent) of the control population also carried the fragment, nearly one-third of those persons were serotyped as DR2. The possible role of gene regulation in the development of IDDM is discussed.
D015139:Blotting, Southern; D015343:DNA Probes, HLA; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D010641:Phenotype; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D015343:DNA Probes, HLA; D006684:HLA-DR Antigens