Expression of four types of human tyrosine hydroxylase in COS cells.
Alternative splicing from a single gene produces four kinds of human tyrosine hydroxylase (types 1-4), which have structural diversity only in the N-terminal region. We attempted expression of the type 1-4 enzymes in COS cells and performed kinetic analyses. All had enzymatic activities. The Km values of the four types for L-tyrosine and 6-methyl-5,6,7,8-tetrahydropteridine were similar, although their relative homospecific activities were clearly different. The type 1 enzyme displayed the highest activity.
D015153:Blotting, Western; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006801:Humans; D007700:Kinetics; D011622:Pterins / Q000378:metabolism; D014162:Transfection; D014443:Tyrosine / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics* / Q000378:metabolism
D011622:Pterins; D014443:Tyrosine; D004247:DNA; C017523:6-methyltetrahydropterin; D014446:Tyrosine 3-Monooxygenase