Extensive co-existence of neuropeptides in the rat visual cortex.
The peroxidase-antiperoxidase immunohistochemical technique has been used to examine the co-existence of peptides within individual neurons of the rat visual cortex. Pairs of consecutive paraffin sections were stained alternately for 2 of the 4 peptides: somatostatin (SRIF), vasoactive intestinal polypeptide (VIP), cholecystokinin (CCK) and neuropeptide Y (NPY). Analysis revealed the co-existence of SRIF with VIP, CCK and NPY and between VIP and CCK. These results show that the co-localization of neuropeptides in cortical neurons is more widespread than previously demonstrated.
D000818:Animals; D002766:Cholecystokinin / Q000032:analysis / Q000502:physiology; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D009478:Neuropeptide Y / Q000032:analysis / Q000502:physiology; D009479:Neuropeptides / Q000032:analysis* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000032:analysis / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000032:analysis / Q000502:physiology; D014793:Visual Cortex / Q000032:analysis* / Q000166:cytology
D009478:Neuropeptide Y; D009479:Neuropeptides; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D002766:Cholecystokinin