Yeast alpha-factor and somatostatin enhance binding of [3H]estradiol to proteins in rat pancreas and Saccharomyces cerevisiae.
Pancreatic tissue contains an [3H]estradiol-binding protein that requires a coligand in the steroid-binding reaction. The endogenous coligand appears to be the tetradecapeptide somatostatin. Yeast alpha-factor, a tridecapeptide pheromone that induces conjugation between haploid cells of opposite mating type, was found to be as effective as somatostatin in enhancing specific binding of [3H]estradiol to partially purified pancreatic protein. Supernatant fractions from yeast cells also contain an [3H]estradiol-binding protein. alpha-Factor can enhance specific binding of [3H]estradiol to such yeast fractions. Somatostatin, somatostatin analogues, and an analogue of alpha-factor enhanced binding of [3H]estradiol but did not inhibit cell growth or induce morphological changes in S. cerevisiae. Thus, it appears that coligand-requiring [3H]estradiol-binding activity and mating in yeast are not directly related.
D000595:Amino Acid Sequence; D000818:Animals; D004958:Estradiol / Q000378:metabolism*; D007700:Kinetics; D000072235:Mating Factor; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011959:Receptors, Estradiol / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013004:Somatostatin / Q000494:pharmacology*
D010455:Peptides; D011506:Proteins; D011959:Receptors, Estradiol; D004958:Estradiol; D013004:Somatostatin; D000072235:Mating Factor