Identification of bovine kappa-casein genotypes at the DNA level.
By using a bovine kappa-Cn cDNA as probe and the PstI endonuclease we demonstrate that the DNA restriction patterns of kappa-Cn AA and kappa-Cn BB cows are different. Besides two invariant fragments (about 6.8kb and 1.1kb) the former shows two fragments of about 4.3 kb and 0.3 kb and the latter one fragment of about 4.6 kb. kappa-Cn AB cows show intermediate pattern. Therefore, it is possible to determine the bovine kappa-Cn genotypes even in absence of gene product.
D000818:Animals; D002364:Caseins / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005260:Female; D005838:Genotype; D012150:Polymorphism, Restriction Fragment Length
D002364:Caseins; D004247:DNA; D004262:DNA Restriction Enzymes