Sulfated oligosaccharides in human lysosomal enzymes.
Cathepsin D, arylsulfatase A and the alpha-chain of beta-hexosaminidase are synthesized in human fibroblasts as sulfated polypeptides. The sulfate is added posttranslationally. Its half-life is less than one-tenth of that of the respective polypeptide chains. The sulfate residues were found on asparagine-linked oligosaccharides sensitive to endoglycosidase F and peptide: N-glycosidase F and resistant to endoglycosidase H. Inhibition of formation of complex type oligosaccharides by 1-deoxy-manno-nojirimycin prevented sulfation, indicating that the sulfate residues were added to complex type oligosaccharides.
D000118:Acetylglucosaminidase; D001216:Asparagine; D002402:Cathepsin D / Q000235:genetics*; D002478:Cells, Cultured; D002553:Cerebroside-Sulfatase / Q000235:genetics*; D003513:Cycloheximide / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology; D006207:Half-Life; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D009844:Oligosaccharides / Q000302:isolation & purification*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D013464:Sulfuric Acids / Q000302:isolation & purification; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics*
D009844:Oligosaccharides; D013464:Sulfuric Acids; D001216:Asparagine; D003513:Cycloheximide; D002553:Cerebroside-Sulfatase; D000118:Acetylglucosaminidase; D001619:beta-N-Acetylhexosaminidases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D002402:Cathepsin D