Liquid chromatographic determination of 9-methyl-3-(1H-tetrazol-5-yl)-4H-pyrido[1,2-a]pyrimidin-4-one in human plasma with fluorescence detection.
A simple and sensitive assay for quantitating 9-methyl-3-(1H-tetrazol-5-yl)-4H-pyrido[1,2-a]pyrimidin-4-one (1; BMY 26517) in human plasma was developed using high-performance liquid chromatography with fluorescence detection. The method involves precipitation of protein and reversed-phase chromatography. The method is linear in the range of 4.3-429 ng/mL of 1, and the limit of detection is 0.4 ng/mL. The day-to-day precision values of this method at 25.7 and 386 ng/mL are 2.1 and 2.6%, respectively. The day-to-day accuracy values at these concentrations are 99.7 and 99.8%, respectively. The recovery of 1 is 98.3%.
D000897:Anti-Ulcer Agents / Q000097:blood*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006635:Histamine H2 Antagonists / Q000097:blood*; D006801:Humans; D011725:Pyridines; D011728:Pyridones / Q000097:blood*; D011744:Pyrimidinones / Q000097:blood*; D013050:Spectrometry, Fluorescence
D000897:Anti-Ulcer Agents; D006635:Histamine H2 Antagonists; D011725:Pyridines; D011728:Pyridones; D011744:Pyrimidinones; C055139:pemirolast