title: cDNA cloning of esterase 1, the major esterase activity in mouse plasma.
abstract: We report here the cloning of a partial cDNA for Esterase 1, the major esterase activity in mouse plasma. A 470 base pair insert was isolated from a lambda gt11 cDNA library constructed from mouse liver poly A+ RNA, and identified by hybrid selected translation. We show that the sexual dimorphism displayed in the plasma levels of this protein is caused by a difference at the level of transcription. In addition, RFLP data using mouse recombinant inbred strains mapped this clone at the Es-1 locus on mouse chromosome 8.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000097:blood / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D004247:DNA; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C469486:Ces1c protein, mouse