Immunohistochemical colocalization of GABA and insulin in beta-cells of rat islet.
gamma-Aminobutyric acid (GABA) is found in high concentrations in the pancreatic islet. In addition, enzymes regulating the level of GABA (L-glutamate decarboxylase and GABA-alpha-ketoglutarate transaminase) have been immunohistochemically localized in the medullary cells of the islet. In this study, an immunofluorescence and elution/restaining protocol is used to determine the distribution of GABA and either insulin, glucagon, or somatostatin in a tissue section. GABA was not detected within the islet alpha- or delta-cells but was determined to be localized within the insulin-containing beta-cells.
D000818:Animals; D005455:Fluorescent Antibody Technique; D005934:Glucagon / Q000032:analysis; D007328:Insulin / Q000032:analysis*; D007515:Islets of Langerhans / Q000166:cytology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D007328:Insulin; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid; D005934:Glucagon