title: Characterization of type 1 and mannose-resistant fimbriae of Erwinia spp.
abstract: Type 1 fimbriae from Erwinia carotovora subsp. carotovora and mannose-resistant fimbriae from Erwinia rhapontici were purified and characterized. The type 1 fimbrillin had an apparent molecular weight of 16,500; that of the mannose-resistant fimbrillin was 18,000. The amino-terminal amino acid sequences of the two fimbrillins were related, but tryptic peptide maps showed significant differences between the proteins. No serological cross-reaction was found between the two fimbrial filaments, nor did they cross-react with type 1 or type 3 fimbriae purified from other enterobacterial species. Immunofluorescent staining of bacterial populations revealed that they were heterogeneous with respect to fimbriation.
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004885:Erwinia / Q000032:analysis* / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000032:analysis* / Q000276:immunology; D008358:Mannose / Q000494:pharmacology; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D010446:Peptide Fragments; D008358:Mannose