title: c-K-ras codon 12 GGT-CGT point mutation. An infrequent event in human lung cancer.
abstract: Hu-c-ras represent a family of oncogenes which are capable of inducing malignant transformation in the NIH/3T3 mouse cell line. Associated with this transformation are specific point mutations observed in the 12th and 61st codon of c-K-ras and N-ras and c-Ha-ras, respectively. These base changes generate, in some instances, a new restriction enzyme cleavage site and a restriction fragment length polymorphism (RFLP). One such RFLP has recently been reported for the mutation GGT-CGT at codon 12 of c-K-ras. Our data suggest that this point mutation is rarely present in human lung cancer and therefore is not likely to play a major role in cancer development.
mesh_terms: D002471:Cell Transformation, Neoplastic / Q000032:analysis*; D003062:Codon / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D009154:Mutation; D009857:Oncogenes; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000302:isolation & purification*
chemical_list: D003062:Codon; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes