title: Corneal inoculation of murine herpesvirus in mice: the absence of neural spread.
abstract: Mouse herpesvirus (MHV) - a recently isolated herpesvirus - when inoculated into the right scarified cornea spread to lungs and liver by haematogenous route. Later on, the virus was recovered from lungs, spleen and trigeminal ganglia at intervals up to 8 months post-infection (p.i.). Another mice were infected by intranasal route and examined up to 264 days p.i. In latter case, MHV was recovered from lungs, spleen, kidneys, trigeminal ganglia and brain stem. At late intervals, direct isolation from lungs and spleen was as frequent as recovery of MHV from cultured explants; kidney fragments in culture yielded virus at a slightly enhanced rate, while positive MHV isolations from trigeminal ganglia and brain stem increased considerably after explantation. It is concluded that the persistence of MHV in lungs and spleen was productive with continuous presence of small but detectable amounts of infectious virus.
mesh_terms: D000818:Animals; D003411:Arvicolinae; D001933:Brain Stem / Q000382:microbiology; D003315:Cornea / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000502:physiology*; D006566:Herpesviridae Infections / Q000382:microbiology*; D007634:Keratitis / Q000382:microbiology*; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D051379:Mice; D009924:Organ Culture Techniques; D013154:Spleen / Q000382:microbiology; D012668:Trigeminal Ganglion / Q000382:microbiology*; D014276:Trigeminal Nerve / Q000382:microbiology*