A population of Thy-1 molecules associated with the cytoskeleton.
Using a monoclonal antibody, SB1 38.456, raised against chicken neuronal Thy-1, we have shown that a significant amount of Thy-1 is associated with the non-ionic detergent-insoluble fraction of chicken brain membranes. Furthermore, this population of Thy-1 is not released by any biochemical method which disrupts microfilaments, microtubules or intermediate filaments, but is released by raising the temperature to 40 degrees C in high ionic strength, cation-containing buffer. We propose, therefore, that a population of Thy-1 molecules is associated with the cytoskeleton either via a linker protein or via other cell surface glycoproteins which are directly associated with the cytoskeleton.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000302:isolation & purification*; D001921:Brain / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D002645:Chickens; D003599:Cytoskeleton / Q000276:immunology*; D009474:Neurons / Q000276:immunology*; D011485:Protein Binding; D013570:Synaptic Membranes / Q000276:immunology; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D018800:Thy-1 Antigens