Phospholipase D activity of Vibrio damsela cytolysin and its interaction with sheep erythrocytes.
Exposure of sheep erythrocytes to sublytic amounts of Vibrio damsela cytolysin markedly reduced their membrane sphingomyelin content and their sensitivity to lysis by the sphingomyelin-dependent cytolysins staphylococcal sphingomyelinase C (beta-toxin) and helianthin. The toxin was found to be a phospholipase D active against sphingomyelin.
D000818:Animals; D003064:Cnidarian Venoms / Q000378:metabolism; D003603:Cytotoxins / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006461:Hemolysis; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D013109:Sphingomyelins / Q000378:metabolism; D013210:Staphylococcus / Q000201:enzymology; D014733:Vibrio / Q000201:enzymology*
D003064:Cnidarian Venoms; D003603:Cytotoxins; D013109:Sphingomyelins; C038137:cytolysin III (Stoichactis helianthus); D010740:Phospholipases; D010739:Phospholipase D