Protection of sheep against footrot with a recombinant DNA-based fimbrial vaccine.
Recombinant Pseudomonas aeruginosa cells containing the Bacteroides nodosus fimbrial subunit gene under the transcriptional control of a strong promoter produce large amounts of B. nodosus-type fimbriae. We have carried out vaccination trials which show that these fimbriae are just as effective as either natural fimbriae or whole cell preparations of B. nodosus in inducing protective immunity against homologous footrot challenge. The recombinant-produced fimbriae are also effective therapeutically in accelerating the rate of healing of pre-existing footrot lesions. These results confirm that the structural subunit of the fimbrial strand is a primary protective antigen against footrot, and demonstrate the practicality and potential of recombinant DNA approaches to the development of new vaccines against B. nodosus and other Type 4 fimbriate pathogens.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001428:Bacterial Vaccines; D001439:Bacteroides / Q000235:genetics / Q000276:immunology*; D004274:DNA, Recombinant; D010861:Fimbriae, Bacterial / Q000276:immunology*; D005535:Foot Rot / Q000517:prevention & control*; D005798:Genes, Bacterial; D008297:Male; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011897:Random Allocation; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary; D014614:Vaccines, Synthetic
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D004274:DNA, Recombinant; D014614:Vaccines, Synthetic