Facilitation of the release of noradrenaline and neuropeptide Y by the alpha 2-adrenoceptor blocking agents idazoxan and hydergine in the dog spleen.
The release of noradrenaline (NA) together with its possible cotransmitter neuropeptide Y (NPY) was investigated in the perfused dog spleen. The splenic nerve was stimulated electrically at high frequency with bursts, which evoked a simultaneous release of both substances. Infusion of the alpha 2-adrenergic blocking agents idazoxan or hydergine enhanced the amount of NA and NPY in the venous effluent. The present results demonstrate the concomitant release of a classical neurotransmitter and a neuropeptide, and suggest that at high frequency stimulation, regulation of their release operates very similarly.
D000317:Adrenergic alpha-Antagonists; D000818:Animals; D004088:Dihydroergotoxine / Q000494:pharmacology*; D004146:Dioxanes / Q000494:pharmacology*; D004147:Dioxins / Q000494:pharmacology*; D004285:Dogs; D004558:Electric Stimulation; D019329:Idazoxan; D009478:Neuropeptide Y / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D013154:Spleen / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*
D000317:Adrenergic alpha-Antagonists; D004146:Dioxanes; D004147:Dioxins; D009478:Neuropeptide Y; D004088:Dihydroergotoxine; D009638:Norepinephrine; D019329:Idazoxan