Nocturnal increase of type II thyroxine 5'-deiodinase activity in the Syrian hamster harderian gland is abolished by light exposure and induced by isoproterenol.
The presence of type II 5'-deiodinase activity in the Syrian hamster Harderian gland was investigated. This enzyme exhibited an increase of its activity after animals entered the normal dark phase, with maximal activity occurring at 04.00 hr (8 hr after lights off). The nocturnal increase was prevented by maintaining the animals in light during the night. Isoproterenol subcutaneously injected every 2 hr (1.0 mg/kg body wt) from 20.00 hr through 0.400 hr to animals exposed to light during the normal dark period mimicked the effect of darkness, i.e., with this treatment an increase in 5'-deiodinase activity with maximal peak values at 02.00 hr was observed. The results show that 5'-deiodinase activity in the Syrian hamster Harderian gland exhibits a nyctohemeral profile dependent on beta-adrenergic activation of the gland.
Guerrero
J M
JM
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Gonzalez-Brito
A
A
Santana
C
C
Reiter
R J
RJ
D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003624:Darkness; D006243:Harderian Gland / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D007453:Iodide Peroxidase / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D007765:Lacrimal Apparatus / Q000201:enzymology*; D008027:Light; D008297:Male; D008647:Mesocricetus
D007453:Iodide Peroxidase; D007545:Isoproterenol