A simple method for direct cloning and sequencing cDNA by the use of a single specific oligonucleotide and oligo(dT) in a polymerase chain reaction (PCR).
Berchtold
M W
MW
Institute of Pharmacology and Biochemistry, University Zürich-Irchel, Switzerland.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D005796:Genes; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D010320:Parvalbumins / Q000235:genetics; D051381:Rats
D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D010320:Parvalbumins; C027903:oligo (dT); D004247:DNA