title: Developmental stage-specific antigens in the nervous system of the cockroach.
abstract: A specific effort was made to obtain monoclonal antibodies that bind to macromolecules that play a role in the development of the nervous system. It was considered that good candidates for such molecules were those that were only transiently present in the embryonic nervous system. Hybridomas were prepared from spleen cells taken from mice that had been immunized with nerve cords from cockroach embryos at the 43-50% stage of development. The hybridoma supernatants were screened for antibody binding to frozen sections of both embryonic and adult thoracic ganglia. Cell lines that produced monoclonal antibodies that transiently bound to the embryonic nervous system were saved and cloned. These developmental stage-specific monoclonal antibodies either did not bind to the adult nervous system or bound to it with a pattern very different from that in the embryonic nervous system. The developmental stage-specific antigens detected by these monoclonal antibodies were organized into four categories based on the part of the embryonic nervous system in which they were transiently localized. These include binding to the cell bodies of all neurons, cell bodies of subsets of neurons or neuroblasts, subsets of axons, and the neuropile. Preliminary biochemical characterization of the antigens showed that many of these antibodies were recognizing carbohydrate epitopes. Functions for these antigens, most of which are components of the cell surface, are tentatively proposed.
authors.affiliation: Biology Department, University of Iowa, Iowa City 52242.
authors.forename: J L
authors.initials: JL
authors.lastname: Denburg
authors.forename: B A
authors.initials: BA
authors.lastname: Norbeck
authors.forename: R T
authors.initials: RT
authors.lastname: Caldwell
authors.forename: J A
authors.initials: JA
authors.lastname: Marner
mesh_terms: D000367:Age Factors; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001369:Axons / Q000276:immunology; D015153:Blotting, Western; D003058:Cockroaches / Q000254:growth & development* / Q000276:immunology; D005724:Ganglia / Q000276:immunology; D006001:Glycoconjugates / Q000276:immunology; D008055:Lipids / Q000276:immunology; D008970:Molecular Weight; D009416:Nerve Regeneration; D009420:Nervous System / Q000254:growth & development / Q000276:immunology*; D009474:Neurons / Q000276:immunology; D011506:Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006001:Glycoconjugates; D008055:Lipids; D011506:Proteins