title: The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
abstract: A component present in splicing extracts selectively binds the 3' splice site of a precursor messenger RNA (pre-mRNA) transcript of a human beta-globin gene. Since this component can be immunoprecipitated by either autoantibodies of the Sm class or antibodies specifically directed against trimethylguanosine, it is a small nuclear ribonucleoprotein (snRNP). Its interaction with the 3' splice site occurs rapidly even at 0 degrees C, does not require adenosine triphosphate, and is altered by certain mutations in the 3' splice site region. Binding is surprisingly insensitive to treatment of the extract with micrococcal nuclease. The U5 particle is the only abundant Sm snRNP with a capped 5' end that is equally resistant to micrococcal nuclease. This suggests that, in addition to the U1 and U2 snRNP's, U5 snRNP's participate in pre-mRNA splicing.
authors.forename: B
authors.initials: B
authors.lastname: Chabot
authors.forename: D L
authors.initials: DL
authors.lastname: Black
authors.forename: D M
authors.initials: DM
authors.lastname: LeMaster
authors.forename: J A
authors.initials: JA
authors.lastname: Steitz
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D005914:Globins / Q000235:genetics; D006801:Humans; D009690:Nucleic Acid Conformation; D009694:Nucleic Acid Precursors / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear; D014158:Transcription, Genetic
chemical_list: D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D005914:Globins