Inhibition by retinoids of in vitro invasive ability of human lung carcinoma cells.
The effect of retinoid treatment of A549 human lung carcinoma cells on in vitro cell invasion using the human amnion basement membrane (BM) was investigated. A 2-day retinoid pretreatment of the cells resulted in a significant reduction in their invasive ability. The most effective retinoid, retinol acetate, inhibited cell migration through the BM and degradation of [3H] proline labeled BM components by 50% at noncytotoxic concentrations of 0.09, and 3 micrograms/ml, respectively. Inhibition by retinol acetate of A549 cell invasive potential was accompanied by a significant decrease in type IV collagenase activity and no change in transglutaminase activity.
Fazely
F
F
Department of Biology, University of Akron, Ohio 44325.
Ledinko
N
N
Smith
D J
DJ
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D004224:Diterpenes; D006801:Humans; D007700:Kinetics; D008175:Lung Neoplasms / Q000201:enzymology / Q000473:pathology*; D003012:Microbial Collagenase / Q000378:metabolism; D009361:Neoplasm Invasiveness; D000084562:Retinyl Esters; D011503:Transglutaminases / Q000378:metabolism; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology
D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C009166:retinol acetate; D011503:Transglutaminases; D003012:Microbial Collagenase