Protein kinase C mediates the hormonally regulated plasma membrane fusion of avian embryonic skeletal muscle.
Recent findings have demonstrated that prostanoid-generated calcium fluxes can trigger myoblast fusion and suggest inositol phospholipid turnover as part of the fusion mechanism. Here we demonstrate that a block imposed on myoblast fusion by antagonists of prostanoid biosynthesis can be overcome by either the membrane-permeable diacylglycerol, 1-oleoyl-2-acetyl-sn-glycerol (OAG), or 12-O-tetradecanoylphorbol 13-acetate (TPA). Both phorbol and the membrane-impermeable dioleoylglycerol were ineffective. These results implicate protein kinase C activation in prostaglandin E1-mediated myoblast fusion and add weight to the contention that inositol turnover is involved in the regulation of myoblast fusion.
Farzaneh
F
F
Department of Obstetrics and Gynaecology, King's College School of Medicine and Dentistry, London, United Kingdom.
Entwistle
A
A
Zalin
R J
RJ
D000527:Alprostadil / Q000378:metabolism; D000818:Animals; D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation; D007213:Indomethacin / Q000494:pharmacology; D009132:Muscles / Q000166:cytology* / Q000196:embryology / Q000201:enzymology; D009218:Myosins / Q000096:biosynthesis; D010704:Phorbols / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004075:Diglycerides; D010704:Phorbols; C038237:1-oleoyl-2-acetylglycerol; D004247:DNA; D011493:Protein Kinase C; D009218:Myosins; D000527:Alprostadil; D013755:Tetradecanoylphorbol Acetate; C033085:phorbol; D007213:Indomethacin; C013965:diolein