title: Cyclic changes in fluorescence polarization of a membrane probe during the cell cycle of an erythroid precursor cell line.
abstract: The membrane lipid bilayer of the K562 cell line undergoes marked changes during cell cycling. These changes can be detected by measuring the fluorescence polarization of the rod-like hydrophobic probe 1,6-diphenyl-1,3,5-hexatriene. Single-cell measurements were performed by flow microfluorometry on synchronized K562 cells. The fluorescence polarization of the probe increased after cell division and was maximal during the S phase. Concomitant with the transition from S to G2 and M phase was a decrease in fluorescence polarization. The data are interpreted as reflecting a minimal membrane lipid fluidity during the S phase and a maximal fluidity during the G2/M-phase of the above erythroid precursor cell line.
authors.affiliation: FACS Laboratory, University Medical Clinic II, Tübingen, Federal Republic of Germany.
authors.forename: C P
authors.initials: CP
authors.lastname: Muller
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D004920:Erythropoiesis; D005454:Fluorescence Polarization; D006412:Hematopoietic Stem Cells / Q000032:analysis*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000032:analysis
chemical_list: D008563:Membrane Lipids