title: The effect of an uptake inhibitor (dihydrokainate) on endogenous excitatory amino acids in the lamprey spinal cord as revealed by microdialysis.
abstract: Microdialysis was utilized to test the effects of the uptake inhibitor dihydrokainate (DHK) on endogenous amino acid levels in the lamprey spinal cord in vitro. The level of L-glutamate increased markedly (165%) in the presence of DHK, whereas the level of the glutamate precursor L-glutamine decreased (53%). Since DHK can potentiate or evoke fictive locomotion in the lamprey spinal cord, it is suggested that L-glutamate is released by neurons which take part in the activation of the spinal locomotor network.
authors.affiliation: Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Brodin
authors.forename: U
authors.initials: U
authors.lastname: Tossman
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
authors.forename: U
authors.initials: U
authors.lastname: Ungerstedt
authors.forename: S
authors.initials: S
authors.lastname: Grillner
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism*; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D007798:Lampreys; D018377:Neurotransmitter Agents / Q000378:metabolism*; D012016:Reference Values; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005971:Glutamates; D018377:Neurotransmitter Agents; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; C020889:dihydrokainic acid; D005680:gamma-Aminobutyric Acid; D007608:Kainic Acid