Mlsa determinants: relationship to Fc gamma receptor and tissue distribution.
We have analysed the genetic relationship between Mlsa and Fc gamma R in mice. Using the Fc gamma R-specific DNA probes, we were unable to detect a restriction fragment length polymorphism (RFLP) which is consistent in DNA derived from Mlsa strains and which differed from that of Mlsb strains, while we could see a polymorphism that distinguishes Ly17.1 from Ly17.2, alleles of the Fc gamma R. These results strongly suggest that Mlsa is neither a product of the alpha Fc gamma R nor of the beta Fc gamma R gene. Furthermore, we have re-examined the tissue distribution of Mlsa determinants using a major histocompatibility complex (MHC) class II antigen-positive T-cell tumour as well as a pure population of bone marrow derived macrophages of Mlsa genotype. Both these cell types were recognized to varying degrees by alloreactive cells; however, none of them expressed functionally detectable Mlsa determinants. We conclude from our studies that Mlsa is a highly stimulatory self peptide that is exclusively expressed in B cells.
Molina
I J
IJ
Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Huber
B T
BT
D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics* / Q000302:isolation & purification; D001402:B-Lymphocytes / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D016747:Minor Lymphocyte Stimulatory Antigens; D012150:Polymorphism, Restriction Fragment Length; D011961:Receptors, Fc / Q000235:genetics*; D017452:Receptors, IgG; D013601:T-Lymphocytes / Q000276:immunology; D014018:Tissue Distribution
D000943:Antigens, Differentiation; D000954:Antigens, Surface; D016747:Minor Lymphocyte Stimulatory Antigens; D011961:Receptors, Fc; D017452:Receptors, IgG