Isolation and partial characterization of keratan sulphate from human brain.
A sulphated glycoconjugate was isolated from adult human brain from a glycosaminoglycan fraction which was not precipitated with 1% cetylpyridinium chloride or ethanol below 50% concentration. It appeared heterogeneous on gel filtration, exhibiting a molecular weight range from about 7000 to over 10 000. Its main covalent structure was shown to contain sulphated, repeating disaccharide units of (beta-D-galactose-(1----4)-N-acetyl-D-glucosamine-(1----3)). In addition, it was susceptible to degradation by keratan sulphate endo-beta-galactosidase and thus was assumed to be keratan sulphate.
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D000328:Adult; D001923:Brain Chemistry; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D006025:Glycosaminoglycans / Q000302:isolation & purification*; D006801:Humans; D007632:Keratan Sulfate / Q000302:isolation & purification*; D008297:Male; D008970:Molecular Weight; D009005:Monosaccharides / Q000302:isolation & purification
D006025:Glycosaminoglycans; D009005:Monosaccharides; D007632:Keratan Sulfate