Microtubule-associated protein-2 stimulates DNA synthesis catalyzed by the nuclear matrix.
Microtubule-associated protein-2 (MAP-2) isolated from porcine brains stimulated DNA synthesis catalyzed by the nuclear matrix isolated from Physarum polycephalum in the presence of activated DNA as exogenous templates. The degree of the stimulation depended on the amount of the nuclear matrix, but not on that of the template. MAP-2 also stimulated DNA polymerase alpha activity solubilized from nuclei, but not DNA polymerase beta activity. These results suggest that MAP-2 stimulates DNA synthesis by interacting with the putative DNA replication machinery including DNA polymerase alpha bound to the matrix. Similar stimulation occurred in the nuclear matrix isolated from HeLa and rat ascites hepatoma cells, which strongly suggests that MAP-2 is involved in the control of DNA replication in eukaryotic cells.
Shioda
M
M
Department of Physiological Chemistry and Nutrition, Faculty of Medicine, Tokyo, Japan.
Murofushi
H
H
Murakami-Murofushi
K
K
Sakai
H
H
D000818:Animals; D002384:Catalysis; D002467:Cell Nucleus / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis*; D004257:DNA Polymerase II / Q000378:metabolism; D004271:DNA, Fungal / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification / Q000502:physiology*; D010804:Physarum; D013552:Swine
D004271:DNA, Fungal; D008869:Microtubule-Associated Proteins; D004247:DNA; D004257:DNA Polymerase II