title: Turbidimetric latex immunoassay of placental lactogen on microtiter plates.
abstract: In this latex immunoassay for human placental lactogen, microtiter plates are used as the reaction vessel and the absorbance at 405 nm is measured to quantify the reactions. This 30-min assay necessitates only one serum dilution and two pipetting steps. The calibration curve extends from 0.5 to 15 mg/L. CVs range from 4.2% to 7.0% for within-run determination and from 7.0% to 11.2% for between-run determinations. A correlation coefficient of 0.949 was obtained for 84 sera when the method was compared with a commercial radioimmunoassay.
authors.affiliation: Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Collet-Cassart
authors.forename: J N
authors.initials: JN
authors.lastname: Limet
authors.forename: L
authors.initials: L
authors.lastname: Van Krieken
authors.forename: R
authors.initials: R
authors.lastname: De Hertogh
mesh_terms: D005260:Female; D006801:Humans; D007118:Immunoassay; D007841:Latex Fixation Tests; D010928:Placental Lactogen / Q000097:blood*; D011247:Pregnancy; D011786:Quality Control; D011863:Radioimmunoassay; D012016:Reference Values; D013053:Spectrophotometry
chemical_list: D010928:Placental Lactogen