title: An in vitro radiolabel uptake viability assay for Onchocerca microfilariae.
abstract: A radiolabel uptake viability assay for Onchocerca cervicalis using [3H]2-deoxy-D-glucose in Hanks' balanced salt solution, pH 7.5, at 30 C is described and compared to the traditional visual motility assay. A correlation of r = 0.92 between the assays was found, with the radiolabel uptake method apparently a more sensitive indicator of microfilarial viability.
authors.affiliation: Department of Ophthalmology, Medical University of South Carolina, Charleston 29425.
authors.forename: H L
authors.initials: HL
authors.lastname: Callahan
authors.forename: J M
authors.initials: JM
authors.lastname: Wakeman
authors.forename: R K
authors.initials: RK
authors.lastname: Crouch
authors.forename: E R
authors.initials: ER
authors.lastname: James
mesh_terms: D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008842:Microfilariae / Q000378:metabolism; D009068:Movement; D009854:Onchocerca / Q000378:metabolism*; D013696:Temperature; D014316:Tritium
chemical_list: D014316:Tritium; D003847:Deoxyglucose