In vitro degradation of opioid peptides by human placental aminopeptidase M.
The degradation of opioid peptides by human placental aminopeptidase M was studied by measuring liberated amino acids by high performance liquid chromatography. The purified placental aminopeptidase M actively degraded Met-enkephalin (Met-Enk) and Leu-enkephalin (Leu-Enk) completely into constituent 5 amino acids. The activities as a function of tyrosine liberation were observed for Met-Enk followed by Leu-Enk, beta-neoendorphin, dynorphin, and beta-endorphin.
Furuhashi
M
M
Department of Obstetrics/Gynaecology, Nagova University School of Medicine, Japan.
Mizutani
S
S
Kurauchi
O
O
Kasugai
M
M
Narita
O
O
Tomoda
Y
Y
D000626:Aminopeptidases / Q000494:pharmacology*; D018826:CD13 Antigens; D004723:Endorphins / Q000378:metabolism*; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D004723:Endorphins; D000626:Aminopeptidases; D018826:CD13 Antigens