Histamine-induced regulation of IL-2 synthesis in man: characterization of two pathways of inhibition.
Histamine at molar concentrations ranging from 10(-5) to 10(-7) exerted an inhibitory effect upon IL-2 synthesis by peripheral blood mononuclear cells in normal man; two pathways of inhibition are described. The first pathway was found to alter the T4 lymphocytes which, in the system used in this study, synthesized nearly 90% of the total IL-2 production and had no suppressive activity. This suggests that histamine can act at the level of IL-2-producing cells. The second pathway of inhibition was related to induction of suppressor cells. Peripheral blood lymphocytes pre-incubated for 1 h with histamine 10(-5)-10(-7) M inhibited the IL-2 synthesis of normal autologous lymphocytes in a co-culture system. This activity was radio-resistant (1200 r) and mediated by T8 lymphocytes. These two pathways of inhibition were mediated by the specific interaction of histamine with H1- and H2-receptor-bearing mononuclear cells.
Huchet
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INSERM U267, Hôpital Paul-Brousse, Villejuif, France.
Grandjon
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D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D016827:CD8 Antigens; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000145:classification / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008213:Lymphocyte Activation / Q000187:drug effects; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000187:drug effects / Q000378:metabolism
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D007376:Interleukin-2; D006632:Histamine; D007213:Indomethacin