Time-resolved fluoroimmunoassay of pancreatic phospholipase A2.
We have developed an immunoassay for human pancreatic secretory phospholipase A2 based on time-resolved fluorescence. The labeled antibody technique in combination with the time-resolved fluorometric detection of the Europium label, which essentially eliminates all background fluorescence, resulted in a high sensitivity and a wide linear range. Our assay is a one-incubation, multi-site, solid-phase assay on polystyrene microtiter strips, even though a polyclonal antibody was used. Increased levels of immunoreactive phospholipase A2 are found by this assay in sera of patients suffering from acute pancreatitis.
Nevalainen
T J
TJ
Department of Pathology, University of Turku, Finland.
Eskola
J U
JU
D000208:Acute Disease; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D010182:Pancreatic Diseases / Q000175:diagnosis* / Q000201:enzymology; D010195:Pancreatitis / Q000175:diagnosis; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2
D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2