Separation of the immune response genes for LDH-B and MOPC-173. III. Evidence that the failure of B10.BASR1 to respond to LDH-B is due to an antigen-presenting cell defect.
Analysis of the immune response of a panel of intra-I-region recombinant mouse strains to LDH-B and MOPC-173 demonstrated that B10.ASR7 (H-2as3) and B10.BASR1 (H-2as4) failed to mount T-cell-proliferative responses to MOPC-173 and LDH-B, respectively. To localize the level of the immune response defect in the B10.BASR1 strain, B10.BASR1 macrophages were shown to be incapable of presenting LDH-B to immune responder B10.ASR7 T cells. These results were confirmed using alloreactivity-depleted and (B10.ASR7 X B10.BASR1)F1 immune T cells. Failure of these strains to respond was shown not to be the result of T cell suppression, because cyclophosphamide and anti-Lyt-2.2-plus-complement treatments did not restore responsiveness. Furthermore, B10.BASR1 macrophages were incapable of educating naive responder T cells in vitro to LDH-B--however, naive nonresponder B10.BASR1 T cells could be educated by responder macrophages to LDH-B in vitro. These results suggest that the failure of B10.BASR1 to respond to LDH-B reflects a defect at the macrophage-T cell interaction level, perhaps related to expression of unique I-A molecules created by intra-I-region recombinatorial events.
Gutmann
D H
DH
Niederhuber
J E
JE
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D005260:Female; D005802:Genes, MHC Class II; D007111:Immunity, Cellular; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase