title: HIV-specific T lymphocyte immunity in mice immunized with a recombinant vaccinia virus.
abstract: Infection by the human immunodeficiency virus (HIV) induces T cell immunity in humans, chimpanzees and macaques. The protective value of this immune response is not clear. We have consequently developed a murine experimental system to study HIV-specific CD4 and CD8 T lymphocyte immunity in vitro and in vivo. BALB/c, DBA/2 and C3H/He mice were immunized with vaccinia virus (VV) recombinant VV-11.39 which expresses the gp160 glycoprotein of HIV-1. Primary and secondary cytotoxic T lymphocyte response to HIV were detected with histocompatible mouse target cells transfected with the HIV-1 env gene. Killer cells were positive for the Thy-1 and Ly-2 (CD8) T cell markers, and were restricted by class I H-2 histocompatibility antigens. Immunological memory specific for HIV-1 envelope antigens was clearly induced by vaccination with VV-11.39:spleen cells from mice vaccinated 4 weeks or more prior to assay generated CD4 and CD8 T lymphocyte responses following stimulation in vitro with HIV envelope antigens. The intensity of these responses increased with consecutive vaccinations, indicating that HIV-specific precursor T cell pools were progressively amplified. Finally, DBA/2 mice vaccinated with VV-11.39 developed protective immunity against a syngeneic tumor which expresses HIV-1 env antigens, leading to accelerated tumor rejection and increased survival.
authors.affiliation: Laboratoire de Biologie et d'Immunologie Moléculaires des Rétrovirus, Institut Pasteur, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Michel
authors.forename: A
authors.initials: A
authors.lastname: Hoffenbach
authors.forename: P
authors.initials: P
authors.lastname: Langlade-Demoyen
authors.forename: B
authors.initials: B
authors.lastname: Guy
authors.forename: M
authors.initials: M
authors.lastname: Girard
authors.forename: J P
authors.initials: JP
authors.lastname: Lecocq
authors.forename: S
authors.initials: S
authors.lastname: Wain-Hobson
authors.forename: M P
authors.initials: MP
authors.lastname: Kieny
authors.forename: F
authors.initials: F
authors.lastname: Plata
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D006678:HIV / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology*; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D051379:Mice; D012191:Retroviridae Proteins / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014616:Vaccinia virus; D014759:Viral Envelope Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D015488:HIV Antigens; D012191:Retroviridae Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines