State-dependent hypotonia in posterior cricoarytenoid muscles of the larynx caused by cholinoceptive reticular mechanisms.
The neural control of the accessory respiratory muscles regulating upper airway patency is poorly understood. This is particularly true with regard to the declines in electromyographic (EMG) activity of upper airway muscles during sleep. To specify the cellular mechanisms causing decreased upper airway muscle tone during sleep, we used an established pharmacological model of rapid eye movement (REM) sleep. With this model, a REM sleep-like state was reliably produced by microinjecting the cholinergic agonist carbachol directly into the pontine reticular formation of the cat. EMG recording were taken from the posterior cricoarytenoid (PCA) muscles of the larynx during wakefulness and the carbachol-induced, REM sleep-like state. This experimental model had not been previously used to study the neuropharmacological control of the upper airway. The results revealed a dose-dependent decrease in PCA muscle tone caused by pontine microinjections of carbachol. To investigate the cholinergic specificity of these effects, the muscarinic cholinergic antagonist pirenzepine was centrally administered before carbachol. Pirenzepine pretreatment effectively blocked the carbachol-induced, REM sleep-like state and attendant changes in muscle tone. These results specify for the first time that muscarinic cholinergic mechanisms within the pontine reticular formation can causally mediate state-dependent hypotonia in accessory respiratory muscles of the upper airway.
Lydic
R
R
Department of Physiology, Pennsylvania State University, College of Medicine, Hershey 17033.
Baghdoyan
H A
HA
Zwillich
C W
CW
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002415:Cats; D004576:Electromyography; D007830:Larynx / Q000502:physiology*; D008297:Male; D010890:Pirenzepine / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000502:physiology*; D012119:Respiration; D012132:Respiratory Muscles / Q000502:physiology*; D012895:Sleep, REM / Q000187:drug effects / Q000502:physiology*
D011950:Receptors, Cholinergic; D010890:Pirenzepine; D002217:Carbachol