Retrograde intrabiliary injection of amphipathic materials causes phospholipid secretion into bile. Taurocholate causes phosphatidylcholine secretion, 3-[(3-cholamidopropyl)dimethylammonio]-propane-1-sulphonate (CHAPS) causes mixed phospholipid secretion.
The control of biliary phospholipid and cholesterol secretions by bile acid was studied by using the technique of retrograde intrabiliary injection. Taurocholate (TC), a moderately hydrophobic bile acid, taurodehydrocholate (TDHC), a hydrophilic non-micelle-forming bile acid, and 3-[(3-cholamidopropyl)-dimethylammonio]propane-1-sulphonate (CHAPS), a detergent, were individually administered by retrograde intrabiliary injection (RII) into the biliary tree, and bile acids, phospholipids and cholesterol subsequently appearing in the bile were measured. TC (1.3 mumol; 45 microliters) injected retrogradely provoked a 3.5-fold increase in biliary phospholipid output for 40 min, as compared with the saline control. Injection of 2.7 mumol of TC (90 microliters) caused a 7.5-fold increase in phospholipid output, which reached a peak at 12 min after RII, and phospholipid output continued for 40 min. Cholesterol output was also elicited under these conditions, showing both dose-dependency and extended secretion. Injection of 1.8 mumol of TDHC caused very little increase in either biliary phospholipid or cholesterol. Injection of 0.9 mumol of CHAPS (45 microliters) provoked a single substantial peak of phospholipid output in the 3 min bile sample. T.l.c. analysis of the phospholipid extracts of the bile collected after each compound showed, for TC, a single compound which co-migrated with the phosphatidylcholine standard, whereas for CHAPS substantial amounts of other phospholipids were present.
Coleman
R
R
Department of Biochemistry, University of Birmingham, U.K.
Rahman
K
K
Kan
K S
KS
Parslow
R A
RA
D000818:Animals; D001646:Bile / Q000187:drug effects*; D001647:Bile Acids and Salts / Q000378:metabolism / Q000494:pharmacology*; D002793:Cholic Acids / Q000494:pharmacology; D008099:Liver / Q000187:drug effects; D008297:Male; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013656:Taurocholic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
D001647:Bile Acids and Salts; D002793:Cholic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; C019560:taurodehydrocholate; D013656:Taurocholic Acid; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate