[Swine serum increases hybridoma stability].
Three hybridoma cell lines producing monoclonal antibodies to lambda phage were cultured for 9 months in Dulbecco's modified Eagl's medium supplemented with 10% fetal calf serum or with 5% swine serum. Under these conditions all the clones had the same proliferation rates and maximum population densities. The cultuvation in the medium with swine serum enhanced the stability of hybridomas: all clones retained the ability to produce antibodies throughout the experiment; one of the clones examined enhanced the activity by 10(3)-10(4) times. When the same three clones were cultured in the medium with the fetal calf serum, two of them lost their ability to produce antibodies 3-15 weeks after the beginning of the experiment.
Mantsygin
Iu A
IuA
Sviatukhina
N V
NV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D010582:Bacteriophage lambda / Q000276:immunology; D002417:Cattle; D006825:Hybridomas / Q000187:drug effects* / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine / Q000276:immunology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D007106:Immune Sera
Svinaia syvorotka povyshaet stabil'nost' gibridom.