Reduced DNase I sensitivity of the rearranged c-myc gene in somatic cell hybrids between murine plasmacytoma cells and fibroblasts.
In mouse plasmacytoma (MPC) S194, the rearranged c-myc gene was much more sensitive to DNase I digestion than the nonrearranged gene. The sensitivity of the rearranged c-myc was markedly reduced to the same extent as that of the nonrearranged one in hybrids between the MPC cells and the fibroblasts, but not in a hybrid between the MPC and the spleen cells. These results suggest that trans-acting factors in fibroblasts alter the DNase I-sensitive structure of the rearranged c-myc gene.
Kondoh
N
N
Laboratory of Molecular Genetics, Hokkaido University School of Medicine, Japan.
Oikawa
T
T
Yuhki
Y
Y
Ogiso
Y
Y
Satoh
C
C
Kuzumaki
N
N
D000818:Animals; D002843:Chromatin / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005347:Fibroblasts; D015321:Gene Rearrangement; D006822:Hybrid Cells; D051379:Mice; D010954:Plasmacytoma; D011519:Proto-Oncogenes
D002843:Chromatin; D003850:Deoxyribonuclease I