Equilibrium binding of spermine and histamine to salmon sperm DNA and poly(dGdC).
Studies using the solute-enhanced phase partition technique demonstrate that the endogenous amines histamine and spermine bind to both salmon sperm DNA and poly(dGdC) in a markedly cooperative fashion. Both compounds exhibited DNA sequence-dependence effects in their mode of binding. The linear aliphatic spermine molecule binds more strongly than histamine to both DNA types.
Winkle
S A
SA
Department of Chemistry, Rutgers University, Piscataway, NJ 08855.
Crooks
P A
PA
D000818:Animals; D004247:DNA / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D012474:Salmon / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*; D013096:Spermine / Q000378:metabolism*
D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); D013096:Spermine; D006632:Histamine; D004247:DNA