Change in chromatin organization of the 3'-flanking region of the rat apoprotein E gene in neonatal rats after an increase in transcriptional activity.
To investigate if a change in chromatin organization is associated with the increased transcriptional activity of the apoprotein E (apo E) gene, a study was done in hepatic nuclei isolated after birth to identify areas in or flanking the rat apo E gene with increased sensitivity to DNase I. An area of preferential digestion in the 3'-flanking region of the apo E gene was identified in liver nuclei from 3-day-old pups but not in nuclei from fetal livers collected at day 20 of gestation. This hypersensitive area may contain information important for the regulated expression of the rat apo E gene.
Fisher
E A
EA
Department of Physiology and Biochemistry, Medical College of Pennsylvania, Philadelphia 19129.
D000818:Animals; D000831:Animals, Newborn / Q000235:genetics* / Q000378:metabolism; D001057:Apolipoproteins E / Q000235:genetics* / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005333:Fetus / Q000378:metabolism; D005786:Gene Expression Regulation; D008099:Liver / Q000196:embryology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D014158:Transcription, Genetic
D001057:Apolipoproteins E; D002843:Chromatin; D003850:Deoxyribonuclease I