title: Solution conformation of sialosylcerebroside (GM4) and its NeuAc(alpha 2----3)Gal beta sugar component.
abstract: The solution conformations of GM4 ganglioside [NeuAc(alpha 2----3)Gal (beta 1----1)Cer] in (2H3C)2SO and its component disaccharide in 2H2O were investigated with the aid of 1H-nuclear magnetic resonance spectroscopy (nuclear Overhauser effect, analysis of coupling constants) and by energy-minimum calculations. The existence of three low-energy conformers obtained by theoretical calculations was supported by experimental findings in the case of GM4, whereas the disaccharide appears to exist as a mixture of two conformers.
authors.affiliation: Max-Planck-Institut für Medizinische Forschung, Heidelberg, Federal Republic of Germany.
authors.forename: L
authors.initials: L
authors.lastname: Poppe
authors.forename: J
authors.initials: J
authors.lastname: Dabrowski
authors.forename: C W
authors.initials: CW
authors.lastname: von der Lieth
authors.forename: M
authors.initials: M
authors.lastname: Numata
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
mesh_terms: D002236:Carbohydrate Conformation; D004187:Disaccharides; D005690:Galactose; D005732:Gangliosides; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008968:Molecular Conformation; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids; D012996:Solutions; D013816:Thermodynamics
chemical_list: D004187:Disaccharides; D005732:Gangliosides; D012794:Sialic Acids; D012996:Solutions; C028253:ganglioside, GM4; D006859:Hydrogen; D019158:N-Acetylneuraminic Acid; D005690:Galactose