A highly conserved sequence on the short arm of chromosome 7 detects multiple polymorphisms.
We have isolated a human DNA fragment (laboratory acronym G98) that detects related sequences in mammals, chicken and Drosophila DNAs. This sequence has been mapped to human chromosome 7 p14-p15 by in situ hybridization. Probe G98 recognizes an insertion-deletion type polymorphism, with allelic frequencies of about 0.5, which can be detected with at least six different restriction enzymes. A second polymorphism, which can be detected in human DNA digested with TaqI, is in non-complete linkage disequilibrium with the first polymorphism. About 70% of the individuals analysed have been found to be heterozygous at this locus.
Bardoni
B
B
Biologia Generale e Genetica Medica, Università di Pavia, Italy.
Guioli
S
S
Maserati
E
E
Maraschio
P
P
Camerino
G
G
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002871:Chromosome Banding; D002897:Chromosomes, Human, Pair 7; D015342:DNA Probes; D004330:Drosophila; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008297:Male; D010375:Pedigree; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D005819:Genetic Markers