title: Observation of the glycocalyx of the organ of Corti: an investigation by electron microscopy in the normal and gentamicin-treated guinea pig.
abstract: The ultrastructure of the glycocalyx of the organ of Corti in the normal and gentamicin-treated guinea pig was investigated at the electron microscopic level by using the ruthenium red staining technique. The glycocalyx was evident over the whole apical surface of both sensory and supporting cells. The entire length of the stereocilia of both inner and outer hair cells was seen to be covered by a glycocalyx, which interconnected the neighbouring stereocilia. In the gentamicin-treated animals, the fusion process between the stereocilia was revealed and a decrease of the glycocalyx was noticed at the point where fusion started. These findings suggest that the glycocalyx may play an important role in holding the stereocilia together in a bundle, yet prevent their close contact by means of its strong negative charge.
authors.affiliation: Department of Otolaryngology, Karolinska Institutet, Karolinska Sjukhuset, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Takumida
authors.forename: J
authors.initials: J
authors.lastname: Wersäll
authors.forename: D
authors.initials: D
authors.lastname: Bagger-Sjöbäck
authors.forename: Y
authors.initials: Y
authors.lastname: Harada
mesh_terms: D000818:Animals; D005839:Gentamicins / Q000494:pharmacology; D006168:Guinea Pigs; D008854:Microscopy, Electron; D009925:Organ of Corti / Q000187:drug effects / Q000648:ultrastructure*
chemical_list: D005839:Gentamicins