Morphologic plasticity of rapid-onset neurites in NG108-15 cells stimulated by substratum-bound laminin.
Undifferentiated NG108-15 cells, when replated onto laminin-coated substrata, extend multipolar, highly branched neurite-like extensions up to 200 microns in length within 4 h; morphologic and pharmacologic properties of these 'rapid-onset neurites' have been described recently. The present study has extended these observations, using time lapse video recordings of their dynamic behavior and additional pharmacologic studies. Rapid-onset neurites and neuronal growth cones were shown to be regulated in an identical manner in all respects examined, including inhibition of outgrowth by cytochalasin B. Of particular interest was the observation that rapid-onset neurites in contact with laminin exhibited an extremely high rate of turnover, which was inhibited by 5'-deoxy-5'-methylthioadenosine (MTA). This system provides a uniquely favorable in vitro preparation in which neuritic plasticity can be elicited, directly observed and experimentally modulated under controlled conditions.
Smalheiser
N R
NR
Department of Pediatrics, University of Chicago, IL 60637.
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism*; D003839:Deoxyadenosines; D005910:Glioma; D007797:Laminin / Q000378:metabolism*; D009447:Neuroblastoma; D013872:Thionucleosides / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D003839:Deoxyadenosines; D007797:Laminin; D013872:Thionucleosides; D003571:Cytochalasin B; C008500:5'-methylthioadenosine; D000241:Adenosine