title: Dilute estradiol implants and progestin receptor induction in the ventromedial nucleus of the hypothalamus: correlation with receptive behavior in female rats.
abstract: The ventromedial nucleus of the hypothalamus (VMN) is thought to constitute an essential neural substrate for hormonal induction of female sexual behavior. In previous work, implants of dilute (0.4%) estradiol (E2) have been found to prime progesterone-facilitated female sexual behavior in rats only when they are within or very close to the VMN. In other studies, induction of cytosolic progestin receptors (PRc) in the hypothalamus has been correlated with systemic E2 priming of receptive behavior. We combined dilute E2 implants with a Palkovits punch microassay for PRc in the an effort to examine the relationship between estrous behavior and estrogen-induced PRc in the VMN of individual female rats. Ovariectomized rats were given bilateral guide cannulae aimed at the VMN and were primed for 3 days with 1) blank implants plus oil sc, 2) blank implants plus 0.5 micrograms E2 benzoate sc, 3) implants of 0.4% E2 in cholesterol, or 4) implants of 2.0% E2. On day 4 all rats received progesterone (P) sc and were tested for receptive behavior. The following week all subjects received the same estrogen-priming treatment but were killed on day 4 without P treatment. The VMN was punch dissected and assayed for PRc. High levels of lordosis were seen in the E2 benzoate-treated and 2.0% E2-implanted animals, while little behavior was seen in the oil-treated animals. Among 0.4% E2-implanted animals, higher levels of lordosis were observed in subjects with implant placements in the rostral than in the caudal VMN; however, lordosis quotients were not correlated with PRc induction measured in the entire VMN of individual animals. In general, our findings suggest that local estrogenic stimulation of target cells in the VMN is capable of both priming the estrous response and induction of P receptors. Whether these two effects are causally related awaits future study.
authors.affiliation: Department of Biological Sciences, Rutgers University, New Brunswick, New Jersey 08903.
authors.forename: E T
authors.initials: ET
authors.lastname: Pleim
authors.forename: T J
authors.initials: TJ
authors.lastname: Brown
authors.forename: N J
authors.initials: NJ
authors.lastname: MacLusky
authors.forename: A M
authors.initials: AM
authors.lastname: Etgen
authors.forename: R J
authors.initials: RJ
authors.lastname: Barfield
mesh_terms: D000818:Animals; D004343:Drug Implants; D004958:Estradiol / Q000008:administration & dosage* / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D010052:Ovariectomy; D051381:Rats; D011919:Rats, Inbred Strains; D011980:Receptors, Progesterone / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D014697:Ventromedial Hypothalamic Nucleus / Q000032:analysis / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D004343:Drug Implants; D011980:Receptors, Progesterone; D004958:Estradiol