Vitellogenin induction by estradiol in channel catfish, Ictalurus punctatus.
In oviparous vertebrates estrogens induce hepatic synthesis of vitellogenin (VG), a blood protein sequestered in vitellogenic oocytes and from which lipovitellin (LV) and phosvitin are derived. Our objective was to identify VG in the channel catfish, Ictalurus punctatus. An intraperitoneal injection of estradiol-17 beta into adult male fish induced a dose-dependent accumulation of a 150 kDa protein (EP) in the plasma. EP was detectable in Coomassie blue-stained polyacrylamide gels within 24 hr after injection of 2 mg hormone/100 g body weight. During the next 4 days, EP increased from 5 to about 25% of the total plasma protein. Electrophoretic mobility, peptide mapping, and immunological crossreactivity showed EP to be indistinguishable from a plasma protein in adult females with vitellogenic ovaries. Two major yolk polypeptides, YP1 (120 kDa) and YP2 (29.6 kDa), were precipitated by (NH4)2SO4 from a yolk protein extract. YP1 but not YP2 reacted with an anti-EP polyclonal antiserum in Western blots. Peptide mapping after proteolysis with trypsin showed YPs 1 and 2 to be unique and revealed structural homologies between YP1 and EP. Liver but not pancreatic explants from an estradiol-treated male synthesized and secreted a [35S]methionine-labeled, 150 kDa protein beginning about 2 hr after initial exposure to the label. We tentatively conclude that EP and YP1 represent VG and LV, respectively. YP2 remains unidentified.
Bradley
J T
JT
Department of Zoology and Wildlife Science, Aquacultures, Auburn University, Alabama.
Grizzle
J M
JM
D000818:Animals; D002397:Catfishes / Q000502:physiology*; D003429:Cross Reactions; D004527:Egg Proteins / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007059:Ictaluridae / Q000502:physiology*; D008297:Male; D012967:Sodium Dodecyl Sulfate; D014819:Vitellogenins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification
D004527:Egg Proteins; D014819:Vitellogenins; D012967:Sodium Dodecyl Sulfate; D004958:Estradiol