title: Cerebrospinal fluid analysis of rabid and vaccinia-rabies glycoprotein recombinant, orally vaccinated raccoons (Procyon lotor).
abstract: Cerebrospinal fluid obtained from clinically normal free-ranging raccoons was analyzed and compared with CSF obtained from raccoons vaccinated orally with vaccinia-rabies glycoprotein (V-RG) recombinant virus vaccine and subsequently challenged peripherally with street rabies virus, and CSF from naive, rabies virus challenge-exposed control raccoons. Significant differences were not found in CSF of free-ranging or V-RG recombinant virus vaccine recipient raccoons, and there was no evidence of CNS invasion by V-RG virus. The CSF of naive, rabies challenge-exposed control raccoons contained high numbers of lymphocytes and monocytes, compatible with rabies virus encephalitis. Although V-RG orally vaccinated challenge-exposed raccoons were protected from lethal rabies virus infection, a mild lymphocytic pleocytosis was evident at 90 days after challenge exposure.
authors.affiliation: Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
authors.forename: C A
authors.initials: CA
authors.lastname: Hanlon
authors.forename: E L
authors.initials: EL
authors.lastname: Ziemer
authors.forename: A N
authors.initials: AN
authors.lastname: Hamir
authors.forename: C E
authors.initials: CE
authors.lastname: Rupprecht
mesh_terms: D000818:Animals; D006023:Glycoproteins / Q000276:immunology; D007958:Leukocyte Count / Q000662:veterinary; D011818:Rabies / Q000134:cerebrospinal fluid / Q000662:veterinary*; D011819:Rabies Vaccines / Q000276:immunology*; D011821:Raccoons / Q000134:cerebrospinal fluid*; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D006023:Glycoproteins; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic