Antigenic proteins of Eimeria maxima gametocytes: cell-free translation and detection with recovered chicken serum.
RNA was extracted from isolated Eimeria maxima gametocytes and translated in a rabbit reticulocyte cell-free protein synthesis system. The major cell-free translation products from E. maxima gametocyte RNA ranged from 225 to 50 kDa, distinct and different from uninfected chicken intestine cell-free translation products. Rabbit antiserum to E. maxima gametocytes as well as recovered chicken sera specifically precipitated some of the major gametocyte cell-free products. A time course of infected intestine RNA indicated that these cell-free synthesized gametocyte antigens appear at 130 to 138 hr postinfection.
Mencher
D
D
Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Pugatsch
T
T
Wallach
M
M
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000235:genetics*; D015153:Blotting, Western / Q000662:veterinary; D002474:Cell-Free System; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary; D004539:Eimeria / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D007411:Intestinal Diseases, Parasitic / Q000276:immunology / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology; D011233:Precipitin Tests / Q000662:veterinary; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics
D000953:Antigens, Protozoan; D012333:RNA, Messenger