Phenotypical and functional characterization of the idiotype-positive blood B cells in multiple myeloma.
In this study the idiotype-positive B cells of one patient with smouldering multiple myeloma (IgG kappa) and of one patient with multiple myeloma (IgG lambda) were analysed phenotypically and functionally. As regards the expression of B cell-associated differentiation antigens and size distribution, the idiotype-positive B cells resembled normal IgG-bearing blood B cells. In functional studies the lymphocytes were cultured in vitro with Staphylococcus aureus, pokeweed mitogen, T-cell factors, or combinations of these. After culture, proliferation and differentiation of the idiotype-positive B cells were measured by autoradiography, an idiotype-specific ELISA, and a spot ELISA. The results show that idiotype-positive B cells of both patients are able to proliferate after stimulation in vitro. In contrast to their normal counterparts, however, almost no increase in the amount of secreted idiotype IgG could be induced. This suggests that the idiotype-positive blood B cells have lost some of their ability to respond to exogenous stimuli.
Bloem
A C
AC
Department of Clinical Immunology, University Hospital, Utrecht, The Netherlands.
Chand
M A
MA
van Camp
B
B
Bast
E J
EJ
Ballieux
R E
RE
D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D018801:CD2 Antigens; D017252:CD3 Complex; D003593:Cytoplasm / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D008213:Lymphocyte Activation; D009101:Multiple Myeloma / Q000097:blood* / Q000276:immunology; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis
D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D017252:CD3 Complex; D006684:HLA-DR Antigens; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic