Induction of the major inducible 68-kDa heat-shock protein after rapid changes of extracellular pH in cultured rat astrocytes.
Cultured rat astrocytes were exposed for 1 or 3 h to acidic medium (pH adjusted to 5.0, 5.5, or 6.0). Radioactive labeling for 3 h after exposure to acidic medium revealed increased synthesis of many proteins, including an inducible 68-kDa protein. Optimal extracellular (medium) pH for the induction of this 68-kDa protein was 5.5. Immunoblotting demonstrated that this 68-kDa protein induced by acidosis was the 68-kDa heat-shock protein previously described in cultured astrocytes.
Nishimura
R N
RN
Department of Neurology, Veterans Administration Hospital, Sepulveda, California 91343.
Dwyer
B E
BE
Cole
R
R
de Vellis
J
J
Picard
K
K
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D051381:Rats
D003470:Culture Media; D006360:Heat-Shock Proteins