Alloreactive T cell responses between HLA-identical siblings. Detection of anti-minor histocompatibility T cell clones induced in vivo.
Cytotoxic T cell (CTL) clones were isolated from the peripheral blood of a patient 13 days following marrow transplantation from her HLA-identical brother. Nineteen of the clones were specifically reactive with host--but not donor--cells, but one clone was reactive with donor but not host cells. Family studies using the 19 clones showed reactivity patterns consistent with three non-HLA, minor histocompatibility antigens (minor-HA). The frequency of reactivity on a large panel of unrelated cells indicated a relatively limited degree of polymorphism. Each of the clones was restricted in its activity by a class I epitope common to HLA-B7, B27, and B40. These data demonstrate that by clonal analysis it is possible to identify in vivo antidonor and antihost alloreactive CTL clones directed against HLA determinants following marrow transplantation.
Irle
C
C
Beatty
P G
PG
Mickelson
E
E
Thomas
E D
ED
Hansen
J A
JA
D000911:Antibodies, Monoclonal; D016026:Bone Marrow Transplantation; D006680:HLA Antigens / Q000276:immunology; D006649:Histocompatibility Antigens / Q000235:genetics*; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008912:Minor Histocompatibility Loci; D010375:Pedigree; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006649:Histocompatibility Antigens