Coordinate expression of MnSOD and CuZnSOD in human fibroblasts.
The amount of manganese superoxide dismutase (MnSOD) and the activity of copper-zinc superoxide dismutase (CuZnSOD) have been studied in five karyotypically normal human fibroblast strains, using nuclear magnetic resonance (NMR) and polarographic methods. A significant correlation between the two enzyme activities, and a linear increase of MnSOD with the increase of CuZnSOD have been demonstrated. Both enzymes are present in nuclei, mitochondria, lysosome-microsome fraction and cytosol. These findings suggest that the two enzymes dismutate the O-2 cooperatively and that a common genetic control maintains the relative amounts of the two enzymes constant.
Crosti
N
N
Bajer
J
J
Serra
A
A
Rigo
A
A
Scarpa
M
M
Viglino
P
P
D000293:Adolescent; D000328:Adult; D002451:Cell Compartmentation; D002460:Cell Line; D002648:Child; D003300:Copper / Q000378:metabolism*; D004314:Down Syndrome / Q000201:enzymology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008345:Manganese / Q000378:metabolism*; D008433:Mathematics; D011048:Polarography; D012867:Skin / Q000166:cytology* / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
D008345:Manganese; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc