Myristic acid is the NH2-terminal blocking group of the 43-kDa protein of Torpedo nicotinic post-synaptic membranes.
The NH2-terminal blocking group of the 43-kDa peripheral membrane protein (43-kDa protein) of Torpedo post-synaptic membranes has been identified as myristic acid. To identify that blocking group pure 43-kDa protein was digested with trypsin and the blocked tryptic peptide was isolated by reverse phase HPLC. That peptide coeluted with and had the same amino acid composition as a synthetic peptide, myristoyl-Gly-Gln-Asp-Gln-Thr-Lys, the structure of the amino terminus predicted from the protein sequence deduced from a cDNA clone. The presence of myristate was confirmed by the precise molecular mass of the peptide, 886.5266, determined by fast atom bombardment mass spectroscopy.
Carr
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Department of Anatomy, Washington University School of Medicine, St. Louis, MO 63110.
Tyler
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AN
Cohen
J B
JB
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D006023:Glycoproteins / Q000302:isolation & purification; D013058:Mass Spectrometry; D008970:Molecular Weight; D019814:Myristic Acid; D009227:Myristic Acids / Q000032:analysis*; D009842:Oligopeptides / Q000138:chemical synthesis; D010446:Peptide Fragments / Q000032:analysis; D011978:Receptors, Nicotinic / Q000302:isolation & purification*; D013570:Synaptic Membranes / Q000378:metabolism*; D014101:Torpedo; D014357:Trypsin
D000596:Amino Acids; D006023:Glycoproteins; D009227:Myristic Acids; D009842:Oligopeptides; D010446:Peptide Fragments; D011978:Receptors, Nicotinic; D019814:Myristic Acid; D014357:Trypsin