title: Dual mechanisms in priming of the chemoattractant-induced respiratory burst in human granulocytes. A Ca2+-dependent and a Ca2+-independent route.
abstract: After interaction with so-called priming agents, the respiratory burst in human granulocytes does not become activated, but is enhanced upon subsequent stimulation with the chemoattractant FMLP. Investigating the mechanism of the priming reaction, we found that a transient rise in the cytosolic free calcium concentration [( Ca2+]i) suffices to irreversibly prime human granulocytes. Thus, platelet-activating factor (PAF) induced a transient increase in [Ca2+]i and primed the cells to an enhanced respiratory burst upon subsequent interaction with FMLP. Artificially, the transient rise in [Ca2+]i was mimicked by addition and subsequent removal of the Ca2+ ionophore ionomycin; this treatment too, primed the respiratory burst of the granulocytes. The priming induced by ionomycin was completely abolished when [Ca2+]i changes were buffered during exposure of the cells to the ionophore. The priming induced by PAF was only partially inhibited under [Ca2+]i-buffering conditions during priming, indicating that multiple pathways exist in the priming of granulocytes by PAF.
authors.affiliation: Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: L
authors.initials: L
authors.lastname: Koenderman
authors.forename: M
authors.initials: M
authors.lastname: Yazdanbakhsh
authors.forename: D
authors.initials: D
authors.lastname: Roos
authors.forename: A J
authors.initials: AJ
authors.lastname: Verhoeven
mesh_terms: D002021:Buffers; D002118:Calcium / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D004987:Ethers / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects; D006801:Humans; D015759:Ionomycin; D007476:Ionophores / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D010101:Oxygen Consumption / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000494:pharmacology
chemical_list: D002021:Buffers; D004987:Ethers; D007476:Ionophores; D010972:Platelet Activating Factor; D015759:Ionomycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium