Macrophage precursors as natural killer cells against tumor cells and microorganisms.
Macrophage precursors cells have been isolated from spleen and liver of mice and have characterized using F4/80 antibody, their proliferative response to CSF-1 and their maturation to macrophages. These nonadherent and nonphagocytic cells exert strong killing of Yac-1 tumor cells and of various microorganisms. Transplantation of these macrophage precursors into lethally irradiated allogenic hosts restores natural killer (NK) activity within 14 days. Macrophage precursors show enhanced NK activity when activated with interleukin 2. FACS analysis of F 4/80 presorted macrophage precursors reveals about 30% of the cells coexpressing NK 1.1. and F 4/80. These data support the assumption that at least a part of the NK cell compartment is derived from the myeloid lineage.
Baccarini
M
M
Department of Immunobiology, Fraunhofer-Institut, Hannover, FRG.
Hao
L
L
Decker
T
T
Lohmann-Matthes
M L
ML
D000818:Animals; D002176:Candida albicans / Q000276:immunology; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D008099:Liver / Q000166:cytology / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011828:Radiation Chimera; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014184:Transplantation, Homologous; D014407:Tumor Cells, Cultured / Q000276:immunology
D003115:Colony-Stimulating Factors; D007376:Interleukin-2