title: XANES spectroscopy sensitivity to small electronic changes. Case of carp azidomethemoglobin.
abstract: Spin states equilibrium of hemoglobin-iron varies with external conditions: pH, allosteric effectors, temperature. The small electronic reorganization of the iron caused by the spin state changes has been detected by X-ray absorption near edge structure (XANES) spectroscopy at room temperature. The iron K-edge region which is sensitive to spin state is located in 7110-7130 eV. Here are presented the 100% high spin and 100% low spin XANES spectra of carp azido ferric hemoglobin.
authors.affiliation: Laboratoire de Biologie Physico-Chimique, Université Paris VII, France.
authors.forename: S
authors.initials: S
authors.lastname: Pin
authors.forename: V
authors.initials: V
authors.lastname: Le Tilly
authors.forename: B
authors.initials: B
authors.lastname: Alpert
authors.forename: R
authors.initials: R
authors.lastname: Cortes
mesh_terms: D000818:Animals; D002347:Carps; D008706:Methemoglobin / Q000031:analogs & derivatives*; D013052:Spectrometry, X-Ray Emission
chemical_list: C029387:methemoglobin azide; D008706:Methemoglobin