Adrenergic, serotoninergic, histaminergic, and imipramine binding sites in post-mortal human cerebral microvessel preparations.
Cerebral microvessels were prepared from fresh and frozen human brain samples obtained from autopsy cases. Structural integrity and purity of the microvessels were confirmed by light and electron microscopy, and by measurement of the enzymatic marker gamma-glutamyltranspeptidase. Similar morphological and enzymatic characteristics were found for the microvessels prepared from fresh and frozen brain samples. Radioligand binding experiments indicated the presence both in the "fresh" and "frozen" microvessel preparations of specific alpha 1-, alpha 2-, and beta-adrenergic, histamine H1, serotonin S1 and imipramine binding sites, although the density of beta-adrenergic and histamine H1 specific binding sites were lower in the frozen samples than in the fresh samples. Low levels of specific binding to muscarinic, GABAergic and serotonin S2 sites (with respect to the specific binding densities in the crude homogenates) were found in the microvessel preparations.
O'Neill
C
C
Department of Geriatric Medicine, Karolinska Institutet, Huddinge, Sweden.
Fowler
C J
CJ
Marcusson
J O
JO
Winblad
B
B
D000328:Adult; D000368:Aged; D002196:Capillaries / Q000378:metabolism; D002352:Carrier Proteins; D002540:Cerebral Cortex / Q000098:blood supply*; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D007099:Imipramine / Q000378:metabolism*; D008833:Microcirculation / Q000378:metabolism; D008875:Middle Aged; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D011955:Receptors, Drug; D011969:Receptors, Histamine H1 / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D002352:Carrier Proteins; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D011955:Receptors, Drug; D011969:Receptors, Histamine H1; D017981:Receptors, Neurotransmitter; D011985:Receptors, Serotonin; C027775:imipramine receptor; D005723:gamma-Glutamyltransferase; D007099:Imipramine