title: Serotonin (5-hydroxytryptamine) turnover in adult female Ascaris suum tissue.
abstract: 1. The 5-hydroxytryptamine (5-HT, serotonin) turnover was examined in the tissues of adult female Ascaris suum. The 5-HT turnover was highest in the intestine at 34.7 ng 5-HT produced/mg protein/hr and 13.8 ng 5-HT produced/mg protein/hr in muscle tissue. 2. The levels of 5-HT metabolites namely tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine, 5-hydroxyindole acetic acid and 5-hydroxytryptophol were measured in muscle and intestinal tissue of adult A. suum. 3. Parachlorophenylalanine inhibited 5-HT production in muscle and intestinal tissue providing in situ evidence for the presence of tryptophan hydroxylase in this tissue. 4. Pargyline increased 5-HT production in muscle and intestinal tissue providing in situ evidence for the presence of monoamine oxidase in this tissue.
authors.affiliation: Department of Biological Sciences, University of North Texas, Denton 76203.
authors.forename: R E
authors.initials: RE
authors.lastname: Martin
authors.forename: J
authors.initials: J
authors.lastname: Chaudhuri
authors.forename: M J
authors.initials: MJ
authors.lastname: Donahue
mesh_terms: D000818:Animals; D001200:Ascaris / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D010477:Perfusion; D012701:Serotonin / Q000378:metabolism*
chemical_list: D012701:Serotonin