Enhanced levels of cathepsin B mRNA in murine tumors.
Relative amounts of mRNA for cathepsin B were measured in normal murine liver and three murine tumors, an invasive liver tumor (hepatoma, Hepa cl 9) and two melanoma variants (B16-F1 and B16 amelanotic melanoma, B16a). Using a human cDNA to the cathepsin B coding region as a hybridization probe, we detected two species of cathepsin B specific RNA transcripts (2.2 and 4.1 kb) in total RNA preparations of all four tissues. The concentrations of the 2.2 and 4.1 kb species were 3.6 and 2.7-fold greater in the highly metastatic B16a melanoma than in normal liver. The concentration of the 2.2 kb species in the invasive hepatoma was 1.7-fold greater than in normal liver. The increased levels of the 2.2 kb message were reflected in increases in activity of cathepsin B in both Hepa cl 9 and B16a.
Moin
K
K
Department of Pharmacology, Wayne State University, Detroit, MI 48201.
Rozhin
J
J
McKernan
T B
TB
Sanders
V J
VJ
Fong
D
D
Honn
K V
KV
Sloane
B F
BF
D000818:Animals; D002401:Cathepsin B / Q000235:genetics*; D015342:DNA Probes; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism*; D009362:Neoplasm Metastasis; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D015342:DNA Probes; D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S; D002401:Cathepsin B