Isolation and characterization of a tumor necrosis factor binding protein from urine.
Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF-BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF-BP was purified 1,000,000-fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF-Sepharose and reverse phase chromatography. The purified protein contained only one chain with an apparent Mr on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 30,000. The aminoterminal amino acid sequence D-S-V-X-P-Q-G-K-Y-I-H-P-Q-V-N-S-I-X-K-T revealed no significant homologies with previously described protein sequences. TNF-BP may act as a regulator of the bioactivities of TNF/cachectin.
Olsson
I
I
Department of Medicine, Lund Hospital, Sweden.
Lantz
M
M
Nilsson
E
E
Peetre
C
C
Thysell
H
H
Grubb
A
A
Adolf
G
G
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000652:urine; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007676:Kidney Failure, Chronic / Q000652:urine*; D008969:Molecular Sequence Data; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism* / Q000652:urine
D002352:Carrier Proteins; D014409:Tumor Necrosis Factor-alpha