Antibody-mediated targeting of differentiation inducers to tumor cells: inhibition of colonic cancer cell growth in vitro and in vivo. A preliminary note.
A differentiation inducer (sodium butyrate) encapsulated in liposomes that are in turn covalently linked to anti-Lex monoclonal antibody, SH1 (IgG3 isotype), was successfully targeted to human colonic adenocarcinoma HRT-18 and HT29 cells expressing Lex antigen in vitro as well as in vivo in athymic nu/nu mice. Tumor cell growth was significantly inhibited and was associated with changes in cell morphology and increases in membrane-bound alkaline phosphatase and gamma-glutamyltranspeptidase, indicating the occurrence of butyrate-induced differentiation.
Otaka
M
M
Biomembrane Institute and University of Washington, Seattle 98119.
Singhal
A
A
Hakomori
S
S
D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology* / Q000628:therapy; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002087:Butyrates / Q000494:pharmacology / Q000627:therapeutic use; D020148:Butyric Acid; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology* / Q000628:therapy; D006801:Humans; D008081:Liposomes / Q000008:administration & dosage; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D002087:Butyrates; D008081:Liposomes; D020148:Butyric Acid