Automated selective dissociation of cells from different regions of multicellular spheroids.
In this report we describe a new apparatus which has been developed for the automated selective dissociation of multicellular spheroids into fractions of viable cells from different locations in the spheroid. This device is based on the exposure of spheroids to a 0.25% solution of trypsin under carefully controlled conditions, such that the cells are released from the outer spheroid surface in successive layers. Study of the spheroid size, number of cells per spheroid, and sections through the spheroid with increasing exposure to trypsin demonstrate the effectiveness of this technique. The technique has been successfully used on spheroids from five different cell lines over a wide range of spheroid diameters. We also present data detailing the effect of varying the dissociation temperature, the mixing speed, the trypsin concentration, and the number of spheroids being dissociated. The new apparatus has several advantages over previous selective dissociation methods and other techniques for isolating cells from different regions in spheroids, including: a) precise control over dissociation conditions, improving reproducibility; b) short time to recover cell fractions; c) ability to isolate large numbers of cells from many different spheroid locations; d) use of common, inexpensive laboratory equipment; and e) easy adaptability to new cell lines or various spheroid sizes. Applications of this method are demonstrated, including the measurement of nutrient consumption rates, regrowth kinetics, and radiation survivals of cells from different spheroid regions.
Freyer
J P
JP
Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Schor
P L
PL
D002448:Cell Adhesion; D002460:Cell Line; D002478:Cells, Cultured / Q000528:radiation effects; D046508:Culture Techniques / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis; D005720:Gamma Rays; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007773:Lactates / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000473:pathology*; D014357:Trypsin / Q000378:metabolism
D004273:DNA, Neoplasm; D007773:Lactates; D014357:Trypsin; D005947:Glucose