title: Translation and processing of Bacillus amyloliquefaciens extracellular RNase.
abstract: Bacillus amyloliquefaciens extracellular RNase has been previously cloned and expressed in Bacillus subtilis. Site-specific mutagenesis experiments have identified codon -39 as the start site of translation. We have determined the primary signal peptide cleavage site of preprobarnase and propose a pathway for the conversion of probarnase to mature barnase.
authors.affiliation: Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
authors.forename: C J
authors.initials: CJ
authors.lastname: Paddon
authors.forename: N
authors.initials: N
authors.lastname: Vasantha
authors.forename: R W
authors.initials: RW
authors.lastname: Hartley
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics*
chemical_list: D003062:Codon; D021382:Protein Sorting Signals; D012260:Ribonucleases