Identification by heteroduplex analysis of an invertible element (Min) common among IncM group plasmids.
The analysis of heteroduplexes between EcoRI digested DNA derived from IncM plasmids in the transfer-off and in the transfer-on state revealed an internal loop of about 1 kbp on a distinct large EcoRI fragment. The presence of an invertible element (Min) is suggested which enables the formation of conjugative pili at 30 degrees C, but switches off the pilus formation at 37 degrees C incubation temperature.
Karste
G
G
Institut für Experimentelle Epidemiologie, Wernigerode, DDR.
Adler
K
K
Klaus
S
S
Tschäpe
H
H
D004269:DNA, Bacterial / Q000032:analysis* / Q000648:ultrastructure; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D010861:Fimbriae, Bacterial / Q000502:physiology*; D008854:Microscopy, Electron; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis* / Q000648:ultrastructure; D010957:Plasmids; D013696:Temperature
D004269:DNA, Bacterial; D009692:Nucleic Acid Heteroduplexes; D015246:Deoxyribonuclease EcoRI