title: Increased regional vascular albumin permeation in the rat during anaphylaxis.
abstract: The changes in vascular albumin permeation induced by systemic anaphylaxis were studied simultaneously in 21 different tissues of the same animal. Before Ag challenge sensitized rats were injected i.v. with 125I-albumin (test tracer), 51Cr-RBC (vascular space marker) and 57Co-EDTA (extravascular space marker). The index of vascular permeation used was the tissue to blood isotope ratio (tbir), which was obtained by dividing the ratio of 125I/51Cr counts in each tissue by the ratio of the same isotopes in the arterial blood sample. After Ag challenge, the increase in the tbir varied considerably among the different tissues. The most pronounced increase was noted in the lymph node (ninefold) followed by the aorta and mesentery (six- to sevenfold) and the various parts of the gastrointestinal tract (four- to sixfold). In the skin less than skeletal muscle less than lung less than liver and eye two- to fourfold increases occurred. Relatively minor increases in albumin permeation (less than twofold) were observed in the brain less than kidney less than heart and less than spleen. The testis was the only organ in which no significant change occurred. For some of the tissues there was also an increase in the tbir for 57Co/51Cr (an index of the extracellular fluid space) suggesting edema formation. The highest increase was noted in the aorta (fourfold). Minor increases occurred in the atrium of the heart, stomach, duodenum, and lymph nodes. There was also a 36% increase in hematocrit. Therefore, systemic anaphylaxis caused extensive extravasation of albumin and hemoconcentration.
authors.affiliation: Department of Pharmacology, Washington University School of Medicine, St. Louis, MO 63110.
authors.forename: W
authors.initials: W
authors.lastname: Leng
authors.forename: K
authors.initials: K
authors.lastname: Chang
authors.forename: J R
authors.initials: JR
authors.lastname: Williamson
authors.forename: B A
authors.initials: BA
authors.lastname: Jakschik
mesh_terms: D000707:Anaphylaxis / Q000097:blood / Q000503:physiopathology*; D000818:Animals; D001794:Blood Pressure; D002199:Capillary Permeability; D002860:Chromium Radioisotopes / Q000493:pharmacokinetics; D003037:Cobalt Radioisotopes / Q000493:pharmacokinetics; D006400:Hematocrit; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D002860:Chromium Radioisotopes; D003037:Cobalt Radioisotopes; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine