Bovine lactogenic immunity against cholera toxin-related enterotoxins and Vibrio cholerae outer membranes.
The newly parturient cow secretes large quantities of immunoglobulin G1, a relatively protease- and heat-resistant immunoglobulin, in its colostrum and milk. This study establishes the feasibility of producing protective colostral immunoglobulins by immunizing pregnant cows with cholera toxin (CT), a CT-related enterotoxin from Escherichia coli, and Vibrio cholerae outer membranes (OMs). The OMs were prepared from bacteria grown under iron-replete or iron-deficient (to simulate the in vivo environment) conditions. Immunoglobulins were purified from the colostrum of newly parturient control and immunized cows. The bovine anti-CT and anti-H-LT (CT-related heat-labile enterotoxin produced by diarrheogenic E. coli strains of human origin) antibodies were quantitated by enzyme-linked immunosorbent assays and by neutralization of toxin activity in both Y-1 adrenal cell and infant rabbit assays. The bovine anti-OM antibodies from both high-iron-grown and low-iron-grown vibrios were assessed by bacterial agglutination and by Western blot (immunoblot) analysis of polyacrylamide gel electrophoresis of high-iron-grown and low-iron-grown OMs. To test their protective effect, immunoglobulin preparations were administered orally in infant feeding formula to 6-day-old rabbits. Anti-CT and anti-OM immunoglobulins elicited statistically significant protection against diarrhea in infant rabbits challenged intraintestinally with virulent cholera vibrios.
Boesman-Finkelstein
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M
Department of Microbiology, School of Medicine, University of Missouri-Columbia 65212.
Walton
N E
NE
Finkelstein
R A
RA
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage* / Q000276:immunology; D015153:Blotting, Western; D002417:Cattle; D002772:Cholera Toxin / Q000276:immunology*; D003967:Diarrhea / Q000517:prevention & control; D004768:Enterotoxins / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D006358:Hot Temperature; D007116:Immunization, Passive; D007743:Labor, Obstetric / Q000276:immunology; D007774:Lactation / Q000276:immunology*; D009500:Neutralization Tests; D011247:Pregnancy; D011817:Rabbits; D014734:Vibrio cholerae / Q000276:immunology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004768:Enterotoxins; D002772:Cholera Toxin