title: Alterations in the sialylation and sulfation of secreted mouse thyrotropin in primary hypothyroidism.
abstract: We investigated the effect of in vivo hypothyroidism on the sialylation and sulfation of thyroid-stimulating hormone (TSH) secreted by mouse pituitary explants. Oligosaccharides from secreted thyroid-stimulating hormone from hypothyroid animals contained greater sialic acid relative to sulfate in both alpha and beta subunits. Aging per se had little effect on thyroid-stimulating hormone sialylation or sulfation. Variable sialylation and sulfation demonstrates a mechanism for charge microheterogeneity of thyroid-stimulating hormone, and the increasing sialylation observed with hypothyroidism may functionally mediate the prolonged metabolic clearance that has been noted previously.
authors.affiliation: Molecular, Cellular, and Nutritional Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
authors.forename: G S
authors.initials: GS
authors.lastname: DeCherney
authors.forename: N
authors.initials: N
authors.lastname: Gesundheit
authors.forename: P W
authors.initials: PW
authors.lastname: Gyves
authors.forename: C R
authors.initials: CR
authors.lastname: Showalter
authors.forename: B D
authors.initials: BD
authors.lastname: Weintraub
mesh_terms: D000375:Aging; D000818:Animals; D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D007037:Hypothyroidism / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009844:Oligosaccharides / Q000378:metabolism; D012794:Sialic Acids / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*; D013972:Thyrotropin / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D012794:Sialic Acids; D013431:Sulfates; D013972:Thyrotropin