Pressure wave injuries to rat dorsal root ganglion cells in culture caused by high-energy missiles.
A high-energy missile impact in an extremity of an animal creates a shock wave which is rapidly dispersed as a burst of oscillating pressure waves that traverses the entire body causing local, regional, and distant injuries. The present study was performed on dorsal root ganglion (DRG) cells, cultured for 3 weeks, to elucidate the cellular mechanism for damage of nerve cells, using a simplified test system. A model system was developed allowing exposure of DRG cultures to a burst of high-frequency oscillating pressure waves, comparable to those recorded in animals after high-energy missile extremity impact. The pressure waves were induced by impact of a high-energy missile in a rubber tube filled with water, in which nerve cell cultures were kept in a closed rubber glove filled with tissue culture medium. The pressure waves had a duration of 0.5-1.5 ms and a frequency spectrum ranging from 0-250 kHz. Within minutes the neurites showed changes in their microtubules. In addition, varicosities, enriched with tubulin immunoreactive material, became irregularly studded along the nerve cell processes. Scattered DRG cells were initially permeable to the marker complex Evans-blue albumin (EBA), used as an indicator of the ability of the plasma membranes to exclude proteins. After 6 hr, however, almost every DRG neuron was intensely stained by EBA. Concomitantly, there was swelling of the nerve cell cytoplasm and organelles, and, to a variable extent, neurofilament tangles were observed.(ABSTRACT TRUNCATED AT 250 WORDS)
Suneson
A
A
Department of Neurosurgery, University of Gothenburg, Sweden.
Hansson
H A
HA
Lycke
E
E
Seeman
T
T
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004735:Energy Transfer; D005727:Ganglia, Spinal / Q000648:ultrastructure*; D007382:Intermediate Filaments / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure; D009991:Oscillometry; D011312:Pressure; D051381:Rats; D011919:Rats, Inbred Strains; D014948:Wounds, Gunshot / Q000473:pathology*