title: H-2-incompatible bone marrow chimeras produce donor-H-2-restricted Ly-2 suppressor T-cell factor(s).
abstract: To study adaptive-differentiation phenomena of T lymphocytes, suppressor T-cell factors (TsF) produced by Ly-2+ splenic T cells from fully allogeneic mouse bone marrow chimeras were analyzed. AKR mice irradiated and reconstituted with B10 marrow cells (B10----AKR chimeras) produced an Ly-2+ TsF after hyperimmunization with sheep erythrocytes. The TsF suppressed primary antibody responses (to sheep erythrocytes) generated with spleen cells of mice of H-2b haplotype but not those of H-2k haplotype. Thus, this suppressor factor was donor-H-2-restricted. The immunoglobulin heavy chain variable region gene (Igh-V)-restricting element was not involved in this form of suppression. Similar results were obtained when TsF from B6----BALB/c and BALB/c----B6 chimeras were analyzed. The TsF from B10----AKR chimeras suppressed responses of B10.A(3R) and B10.A(5R) mice but not those of B10.A(4R). This finding showed that identity between the factor-producing cells and target spleen cells is required on the left-hand side of the E beta locus of the H-2 region and that the putative I-Jb locus is not involved in this form of suppression. The present results support the postulate that post-thymic differentiation in the presence of continued or repeated stimulation with antigen and donor-derived antigen-presenting cells generates donor-H-2-restricted T-cell clones that may predominate within the repertoire of the specific antigen being presented.
authors.forename: M
authors.initials: M
authors.lastname: Noguchi
authors.forename: K
authors.initials: K
authors.lastname: Onoé
authors.forename: M
authors.initials: M
authors.lastname: Ogasawara
authors.forename: K
authors.initials: K
authors.lastname: Iwabuchi
authors.forename: L
authors.initials: L
authors.lastname: Geng
authors.forename: K
authors.initials: K
authors.lastname: Ogasawara
authors.forename: R A
authors.initials: RA
authors.lastname: Good
authors.forename: K
authors.initials: K
authors.lastname: Morikawa
mesh_terms: D000483:Alleles; D000818:Animals; D000950:Antigens, Ly / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology* / Q000528:radiation effects; D002678:Chimera; D004912:Erythrocytes / Q000276:immunology; D008040:Genetic Linkage; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006736:Horses; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D000950:Antigens, Ly; D006183:H-2 Antigens