title: A zonal pattern of neuroglial cells during the development of the intermediate lobe of the rat pituitary.
abstract: In this paper the development of the intermediate lobe (IL) of the pituitary of the rat is described using alkaline phosphatase (AP) (EC 3.1.3.1.) enzyme histochemistry and stage-specific embryonic antigen 1 (SSEA-1) immunocytochemistry. SSEA-1 and AP are co-localized during late development and reveal the existence of two cytochemically different cell types within the IL, i.e. SSEA-1/AP-positive and SSEA-1/AP-negative cells. The SSEA-1/AP-positive cells are initially arranged along the hypophyseal lumen, in a number of longitudinally oriented zones. alpha-Melanocyte-stimulating hormone (alpha-MSH) immunoreactivity is expressed in the SSEA-1/AP-negative cells from E20 onwards. Eventually the SSEA-1/AP-positive cells develop into a layer of cells covering the luminal surface of the IL lobules. These cells represent the glio-epithelial or neuroglial cells of the IL.
authors.affiliation: Laboratory of Anatomy and Embryology, University of Leyden, The Netherlands.
authors.forename: E A
authors.initials: EA
authors.lastname: Lakke
authors.forename: E
authors.initials: E
authors.lastname: Marani
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D006017:Glycolipids / Q000032:analysis*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D016256:Lewis X Antigen; D009457:Neuroglia / Q000032:analysis* / Q000166:cytology; D010902:Pituitary Gland / Q000196:embryology*; D051381:Rats; D000521:alpha-MSH / Q000032:analysis*
chemical_list: D006017:Glycolipids; D016256:Lewis X Antigen; D000521:alpha-MSH; D000469:Alkaline Phosphatase