Iron exerts a specific inhibitory effect on CD2 expression of human PBL.
The effect of in vitro exposure to ferric citrate (Fe-citrate) on the expression of human lymphocyte surface markers was studied. The following markers were examined: E-rosette formation, CD2, CD3, CD4, CD8, CD1, CD22, CD10 and HLA-DR. Pretreatment of peripheral blood lymphocytes (PBL) with Fe-citrate at concentrations ranging from 10(-2) M to 10(-5) M resulted in the exclusive inhibition of E-rosette formation and CD2 expression. None of the other surface antigens examined appeared to be sensitive to the Fe-citrate treatment. Competition experiments further indicated that iron interacts specifically with CD2 on T lymphocytes.
Carvalho
G S
GS
Department of Biology, University of Aveiro, Portugal.
de Sousa
M
M
D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D004912:Erythrocytes / Q000276:immunology; D005290:Ferric Compounds / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D011971:Receptors, Immunologic; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D005290:Ferric Compounds; D011971:Receptors, Immunologic; C025314:ferric citrate; D007501:Iron