title: Anti-bovine beta-lactoglobulin antibodies react with a human lactoferrin fragment and bovine beta-lactoglobulin present in human milk.
abstract: Human milk samples react against anti-bovine beta-lactoglobulin rabbit antibodies, as measured by a competitive radioimmunoassay. Immunoreactivity was positive even in milk from mothers consuming a diet free of cow's milk. An increase with a diet rich in cow's milk proteins was detected by immunoelectrophoresis. The human milk fraction cross-reacting with anti-bovine beta-lactoglobulin antibodies corresponds to the 20 kDa fragment from the N-terminal end of human lactoferrin. Three regions of this fragment exhibit sequence homology with a sequence contained in cow's beta-lactoglobulin (between residues 124 and 141).
authors.affiliation: Nestlé Research Centre, Nestec Ltd., Lausanne, Switzerland.
authors.forename: J C
authors.initials: JC
authors.lastname: Monti
authors.forename: A F
authors.initials: AF
authors.lastname: Mermoud
authors.forename: P
authors.initials: P
authors.lastname: Jollès
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007122:Immunoelectrophoresis; D007781:Lactoferrin / Q000032:analysis* / Q000276:immunology; D007782:Lactoglobulins / Q000032:analysis* / Q000276:immunology; D008892:Milk / Q000032:analysis; D008895:Milk, Human / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments; D011863:Radioimmunoassay; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007782:Lactoglobulins; D010446:Peptide Fragments; D007781:Lactoferrin