Failure of antioxidant therapy (polyethylene glycol-conjugated catalase) in acute pancreatitis.
Previous studies have demonstrated that intravenous catalase infusion protects against the formation of pancreatic edema in cerulein-induced acute pancreatitis; however, polyethylene glycol (PEG)-conjugated catalase given as a bolus was not protective. Using radiolabeled catalase and PEG-catalase in subtherapeutic tracer doses, the pancreas tissue distributions of each were determined in rats with and without pancreatitis. Rats with cerulein-induced pancreatitis developed tissue concentrations of catalase within the pancreas that were three times those of PEG-catalase. The relatively low levels of PEG-catalase in the pancreas outside of the vascular compartment suggest that the failure to prevent edema formation may result from inability of PEG-catalase to reach extravascular sites of injury because of the large molecular size.
Guice
K S
KS
Department of Surgery, University of Michigan Medical School, Ann Arbor 48109.
Oldham
K T
KT
Johnson
K J
KJ
D000208:Acute Disease; D000818:Animals; D002374:Catalase / Q000378:metabolism / Q000627:therapeutic use*; D002108:Ceruletide; D004338:Drug Combinations; D004487:Edema / Q000517:prevention & control*; D008297:Male; D010179:Pancreas / Q000378:metabolism; D010182:Pancreatic Diseases / Q000517:prevention & control*; D010195:Pancreatitis / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000378:metabolism / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains
D004338:Drug Combinations; D011092:Polyethylene Glycols; D002108:Ceruletide; D002374:Catalase