Antibody-complement-mediated cytotoxicity is enhanced by ribosome-inactivating proteins.
The antibody-mediated cytotoxicity of complement can be increased by the ribosome-inactivating proteins gelonin and PAP-S. Treatment of human lymphoid cells that express CALLA with an anti-CALLA monoclonal antibody, J5, and then with rabbit complement, leaves about 6% of the cells alive. The same treatment in the presence of a sublethal concentration of gelonin or PAP-S leaves only about 0.02 and 0.3% of the cells alive respectively. This synergistic effect has potential implications for the in vitro elimination of malignant cells or of immunocompetent cells from bone marrow before its transplantation.
Goldmacher
V S
VS
Anderson
J
J
Blättler
W A
WA
Lambert
J M
JM
Senter
P D
PD
D000920:Antibody-Dependent Cell Cytotoxicity; D000951:Antigens, Neoplasm / Q000276:immunology; D000998:Antiviral Agents / Q000494:pharmacology*; D002051:Burkitt Lymphoma / Q000276:immunology; D002460:Cell Line; D003165:Complement System Proteins / Q000502:physiology*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology; D009699:N-Glycosyl Hydrolases; D015260:Neprilysin; D010940:Plant Proteins / Q000494:pharmacology*; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors; D054789:Ribosome Inactivating Proteins, Type 1
D000951:Antigens, Neoplasm; D000998:Antiviral Agents; D010940:Plant Proteins; D012269:Ribosomal Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum; D003165:Complement System Proteins; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein; D015260:Neprilysin