Direct immunization with synthetic peptidyl-polyamide resin. Comparison with antibody production from free peptide and conjugates with carrier proteins.
An 11-amino acid residue peptidyl-linkage agent-polyamide resin complex was synthesized by the fluorenylmethyloxycarbonyl (Fmoc)-polyamide solid-phase system. Mice were immunized with the free peptide, peptidyl-resin and peptide coupled to the carrier proteins ovalbumin (Ova) and keyhole limpet haemocyanin (KLH). The immunogenicity of these materials was assessed by measurement of the capacity of the various antisera to bind the peptide in an enzyme-linked immunosorbent assay (ELISA). The peptidyl-resin exhibited enhanced immunogenicity compared to the free peptide. It is suggested that the time needed for screening for immunogenicity of large numbers of synthetic peptides thus be greatly shortened by using peptidyl-resins for immunization. This method eliminates laborious cleavage of peptide from resin, purification, coupling to carrier and the difficulties of handling peptides of low solubility.
Fischer
P M
PM
Division of Biological and Health Sciences, Deakin University, Victoria, Australia.
Comis
A
A
Howden
M E
ME
D000818:Animals; D000917:Antibody Formation; D006433:Hemocyanins / Q000276:immunology; D007114:Immunization; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D012116:Resins, Plant; D013329:Structure-Activity Relationship; D013744:Tetanus Toxin / Q000276:immunology*
D010455:Peptides; D012116:Resins, Plant; D013744:Tetanus Toxin; D010047:Ovalbumin; D006433:Hemocyanins