title: HLA class II genes polymorphism in DR4 giant cell arteritis patients.
abstract: We have previously reported a significant increase of HLA-DR4 antigen frequency in giant cell arteritis (GCA). This finding suggested an important role of immunogenetic factors in this syndrome. Recent data suggest that inherited susceptibility to several autoimmune diseases was associated with specific DR4 associated DQ beta alleles. DNAs from 27 DR4 positive patients with GCA were digested with Taq I and Bam HI, analysed on 0.7% agarose gel and hybridized with DR beta, DQ alpha and DQ beta probes. DR beta hybridization produced no variant detectable within DR4. DQ beta probe confirmed two clusters among DR4 associated DQW3 alleles: DQW 3.1 (Bam HI 360 Kb) and DQw 3.2 (Taq I 1.9 Kb and Bam HI 11 Kb). Among our 27 DR4 positive patients, 34% were DQW 3.1 and 66% were DQW 3.2. These frequencies are the same as those observed in healthy controls.
authors.affiliation: Laboratoire d'histocompatibilité, Centre Régional de Transfusion Sanguine, Nantes, France.
authors.forename: J D
authors.initials: JD
authors.lastname: Bignon
authors.forename: C
authors.initials: C
authors.lastname: Ferec
authors.forename: J
authors.initials: J
authors.lastname: Barrier
authors.forename: Y
authors.initials: Y
authors.lastname: Pennec
authors.forename: C
authors.initials: C
authors.lastname: Verlingue
authors.forename: M L
authors.initials: ML
authors.lastname: Cheneau
authors.forename: V
authors.initials: V
authors.lastname: Lucas
authors.forename: J Y
authors.initials: JY
authors.lastname: Muller
authors.forename: J P
authors.initials: JP
authors.lastname: Saleun
mesh_terms: D004247:DNA / Q000032:analysis; D013700:Giant Cell Arteritis / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics*; D015804:HLA-DR4 Antigen; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; D000949:Histocompatibility Antigens Class II; D004247:DNA