The interactions of bilirubin with model and biological membranes.
The partitioning of bilirubin between albumin and model and biological membranes and the differential partitioning of bilirubin between membranes with different lipid and protein compositions were measured. Partition coefficients were independent of the concentration of bilirubin in membranes up to at least 7 mol of bilirubin/mol of phospholipid. The avidity of albumin for bilirubin was greater than that of membranes, but the avidity of the latter for bilirubin depended on the composition of the membrane. Bilirubin partitioned preferentially into model membranes comprised of microsomal lipids greater than dioleoylphosphatidylcholine = plasma membrane lipids much greater than egg phosphatidylcholine = dimyristoylphosphatidylcholine. Partitioning into membranes was increased if these contained proteins, but the effect of proteins could not be attributed to specific binding to sites on proteins, as reflected by the temperature independence of partition coefficients. Differential partitioning of bilirubin into different membranes of pure lipids also was independent of temperature. Differences in the bulk phase fluidity of membranes does not appear to account for the preferential partitioning of bilirubin into some membranes. It appears that bilirubin partitions into elements of free volume of differing sizes in membranes with variable lipid compositions and that the size of these elements can be increased by adding proteins to membranes.
Leonard
M
M
Department of Medicine, Cornell University Medical College, New York, New York 10021.
Noy
N
N
Zakim
D
D
D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008567:Membranes, Artificial; D008861:Microsomes / Q000378:metabolism; D010713:Phosphatidylcholines; D051381:Rats; D013329:Structure-Activity Relationship
D008563:Membrane Lipids; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D001663:Bilirubin