title: Polyadenylation of histone H3 and H4 mRNAs in dicotyledonous plants.
abstract: The histone H3 and H4 genes are shown to be expressed in both Arabidopsis plantlets and transitory multicellular suspension. The 5'- and 3'-ends of the H4 mRNAs have been localized on two H4 genes previously sequenced, H4A748 and H4A777. S1-nuclease mapping and reverse-transcriptase-primer-elongation experiments revealed the existence of two start points for transcription, located 31 and 37 nucleotides downstream from the TATA-box. The 3'-end of the mRNA corresponding to H4A748 was localized at 177 nt after the stop codon. The other gene, H4A777, most probably is not expressed. In addition to a long 3'-untranslated region, the H4 mRNA was shown to be polyadenylated in both plantlets and cell-suspension. This observation was extended to the H3 mRNAs of Arabidopsis and of two other dicots, tobacco and sunflower. Previous results on maize H3 and H4 mRNAs suggest that polyadenylation is a common feature for histone mRNAs in higher plants.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg, France.
authors.forename: M E
authors.initials: ME
authors.lastname: Chaboute
authors.forename: N
authors.initials: N
authors.lastname: Chaubet
authors.forename: B
authors.initials: B
authors.lastname: Clement
authors.forename: C
authors.initials: C
authors.lastname: Gigot
authors.forename: G
authors.initials: G
authors.lastname: Philipps
mesh_terms: D001483:Base Sequence; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D006657:Histones; D012333:RNA, Messenger; D011061:Poly A