title: Biosynthesis and secretion of proatrial natriuretic factor by cultured rat cardiocytes.
abstract: Rat atrial natriuretic factor (ANF) is translated as a 152-amino acid precursor preproANF. PreproANF is converted to the 126-amino acid proANF, the storage form of ANF in the atria. ANF isolated from the blood is approximately 25 amino acids long. It is demonstrated here that rat cardiocytes in culture store and secrete proANF. Incubation of proANF with serum produced a smaller ANF peptide. PreproANF seems to be processed to proANF in the atria, and proANF appears to be released into the blood, where it is converted by a protease to a smaller peptide.
authors.forename: K D
authors.initials: KD
authors.lastname: Bloch
authors.forename: J A
authors.initials: JA
authors.lastname: Scott
authors.forename: J B
authors.initials: JB
authors.lastname: Zisfein
authors.forename: J T
authors.initials: JT
authors.lastname: Fallon
authors.forename: M N
authors.initials: MN
authors.lastname: Margolies
authors.forename: C E
authors.initials: CE
authors.lastname: Seidman
authors.forename: G R
authors.initials: GR
authors.lastname: Matsueda
authors.forename: C J
authors.initials: CJ
authors.lastname: Homcy
authors.forename: R M
authors.initials: RM
authors.lastname: Graham
authors.forename: J G
authors.initials: JG
authors.lastname: Seidman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001345:Autoradiography; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006321:Heart / Q000502:physiology; D007106:Immune Sera / Q000276:immunology; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits / Q000276:immunology; D051381:Rats
chemical_list: D007106:Immune Sera; D011498:Protein Precursors; D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor