Two different preparations of subfragment-1 from scallop adductor myosin.
Chymotryptic digestion of scallop myosin yielded two different preparations of subfragment-1, having the following features. The major product from chymotryptic digestion of scallop myosin was subfragment-1 (S1) either in Ca-medium or in EDTA-medium. However, the S1 preparations obtained from the digestion in Ca-medium, abbreviated as Ca-S1(CT), had both types of light chain subunits (regulatory light chains (R-LC) and essential light chains (SH-LC], and 100 Kdaltons (Kd) heavy chain subfragments (HCs), whereas the S1 preparations obtained from the digestion in EDTA-medium, ED-S1(CT), had no R-LC, partially fragmented SH-LC (SH-LC), and 90 Kd HCs. On the other hand, Ca-S1(CT) and ED-S1(CT) were practically identical with each other in ATPase activity and in actin-binding ability. The two S1 preparations were also identical in that the Mg-ATPase activity of both S1 and acto-S1 was insensitive to calcium ions. Ca-S1(CT), which contained both R-LC and SH-LC in a stoichiometric amount, was further digested with trypsin, which is known to cleave rabbit skeletal myosin not only at the head-tail junction but also in the head. The tryptic digestion of Ca-S1(CT) appeared, in terms of the SDS-gel electrophoretic pattern, to occur at a much faster rate in Ca-medium than in EDTA-medium, and with a different digestion profile. It is therefore suggested that association of R-LC induces changes in the heavy chain conformation which result in an increase in the proteolytic digestibility of heavy chains and in an alteration of the site of proteolytic cleavage on heavy chains.
Konno
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K
Watanabe
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D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D046911:Macromolecular Substances; D008970:Molecular Weight; D008974:Mollusca / Q000032:analysis*; D009132:Muscles / Q000032:analysis*; D015879:Myosin Subfragments; D009218:Myosins / Q000302:isolation & purification* / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000378:metabolism; D013329:Structure-Activity Relationship
D000199:Actins; D046911:Macromolecular Substances; D015879:Myosin Subfragments; D010446:Peptide Fragments; D002918:Chymotrypsin; D000251:Adenosine Triphosphatases; D009218:Myosins; D002118:Calcium