title: Age-related changes in retinal sensitivity, rhodopsin content and rod outer segment length in hooded rats following low-level lead exposure during development.
abstract: Electroretinographic, morphometric and cyclic nucleotide metabolism studies in adult hooded rats have established that low-level lead exposure during early postnatal development (postnatal days 0-21) causes long-term selective rod deficits and degeneration. To determine if this same low-level lead exposure during early postnatal development produces immediate and/or long-term alterations in retinal sensitivity we examined ERG b-wave threshold responses in dark-adapted control and lead-exposed rats at 1-, 3- and 12 months of age. In addition, to determine possible sites and mechanisms of action responsible for the observed decreases in retinal sensitivity we analyzed the rhodopsin content per eye, the lambda max of rhodopsin and rod outer segment (ROS) length in superior and inferior posterior retina at 1-, 3- and 12 months of age. Relative to adult (3-month-old) controls whose log threshold was arbitrarily set at 0 log units, the mean log relative threshold in control rats was 0.4 log units at 1 month of age and 0.2 log units at 1 yr of age. In contrast, the mean log relative threshold in lead-exposed rats was 1.3 log units at 1 month of age and 1.1-1.2 log units at 3- and 12 months of age. Thus, compared with controls, retinal sensitivity in lead-exposed rats was decreased approx. 1 log unit at all ages examined. The rhodopsin content per eye in control rats increased 13% between 1- and 3 months of age, reaching an adult value of 1.99 nmol per eye, and then decreased 8% by 1 yr of age. In contrast, the eyes from lead-exposed rats contained 30-34% less rhodopsin at all ages examined. No change in the lambda max of rhodopsin was observed in the retinas from the lead-exposed rats. In both controls and lead-exposed rats, the developmental changes in log b-wave relative threshold were paralleled by linear increases and decreases in rhodopsin content per eye such that a log-linear relation between retinal sensitivity and rhodopsin content per eye existed between 1- and 12 months of age. The developmental changes in the superior and inferior retinal ROS length were similar in control and lead-exposed rats: ROSs were at their adult length at 1 month of age and then slightly decreased by 1 yr of age.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: College of Optometry, University of Houston, TX 77204.
authors.forename: D A
authors.initials: DA
authors.lastname: Fox
authors.forename: S D
authors.initials: SD
authors.lastname: Rubinstein
mesh_terms: D000375:Aging; D000818:Animals; D004596:Electroretinography; D005260:Female; D007854:Lead / Q000097:blood / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000187:drug effects* / Q000378:metabolism; D012168:Retinal Pigments / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism*; D012374:Rod Cell Outer Segment / Q000166:cytology / Q000187:drug effects
chemical_list: D012168:Retinal Pigments; D007854:Lead; D012243:Rhodopsin