title: Protection of turkey poults from Bordetella avium infection and disease by pili and bacterins.
abstract: The role of pili in protection against Bordetella avium infection in turkey poults was studied. An isolate that produced the largest number of pili under growth conditions developed in our laboratory was used for preparation of pili and bacterin and for challenge. The pili were isolated, purified, examined by electron microscopy, and tested for purity by gel electrophoresis. Poults were vaccinated with oil-adjuvant pili, formaldehyde- or merthiolate-inactivated bacterins, or a commercial bacterin. Poults were vaccinated once or twice subcutaneously at different ages and challenged intranasally with a pathogenic B. avium isolate 5 days following the last vaccination. A few vaccinated birds had very mild clinical signs. B. avium was isolated from the sinuses of a few vaccinated birds, and growth was scanty. The mean colony counts from tracheal sections was significantly higher (P less than 0.1) in unvaccinated challenged poults than in vaccinated challenged poults. It is postulated that B. avium pili are important immunogens in turkey poults.
authors.affiliation: Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
authors.forename: M A
authors.initials: MA
authors.lastname: Akeila
authors.forename: Y M
authors.initials: YM
authors.lastname: Saif
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D001885:Bordetella Infections / Q000517:prevention & control / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology*; D009296:Nasal Cavity / Q000382:microbiology; D011201:Poultry Diseases / Q000517:prevention & control*; D014132:Trachea / Q000382:microbiology; D014422:Turkeys; D015164:Vaccines, Inactivated / Q000008:administration & dosage
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D015164:Vaccines, Inactivated