Cadmium sensitivity differences between liver microsomal drug metabolizing enzyme systems of guinea-pig and rat.
1. Male guinea-pigs (400-500 g) and rats (225-275 g) were given a single dose of cadmium chloride (CdCl2) (2 mg Cd2+/kg i.p.) and 72 hr later the liver microsomal drug metabolizing enzyme activities and Cd levels of tissues and microsomes were determined. 2. No significant differences were noted between Cd treated and control animal tissue weights of microsomal protein contents in either guinea-pigs or rats. 3. Cd treatment exhibited significant inhibition of the activities of aniline 4-hydroxylase and ethylmorphine N-demethylase and on the levels of cytochrome P-450 and cytochrome b5 of liver of both species but the degree of inhibition were not the same in the species; they were 23, 34, 16 and 10% in guinea-pigs and 58, 57, 25 and 13% in rats, respectively. 4. No activity changes were observed in liver NADPH-cytochrome c reductase of the species by Cd treatment. 5. The duration of hexobarbital sleeping time was significantly prolonged in both species. However, the prolongation was 1.6 fold in guinea-pigs but 3.4 fold in rats. 6. No significant differences were found between either tissue or microsomal Cd levels of guinea-pigs and rats.
Işcan
M
M
Department of Toxicology, Faculty of Pharmacy, Ankara University, Turkey.
Karakaya
A
A
D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003573:Cytochrome b Group / Q000378:metabolism; D015786:Cytochromes b5; D004347:Drug Interactions; D005037:Ethylmorphine-N-Demethylase / Q000378:metabolism; D006168:Guinea Pigs; D006591:Hexobarbital / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D051381:Rats; D012890:Sleep / Q000187:drug effects; D013045:Species Specificity; D014018:Tissue Distribution
D003573:Cytochrome b Group; D002104:Cadmium; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D006591:Hexobarbital; D000815:Aniline Hydroxylase; D005037:Ethylmorphine-N-Demethylase; D009251:NADPH-Ferrihemoprotein Reductase