Energy-induced modulation of the kinetics of oxidative phosphorylation and reverse electron transfer.
The kinetics of ATP synthesis by bovine heart submitochondrial particles (SMP) are modulated by the rate of energy production by the respiratory chain between two fixed limits characterized by apparent KmADP = 2-4 microM and Vmax approximately 200 nmol of ATP min-1 (mg of SMP protein)-1 at low energy levels and apparent KmADP = 120-160 microM and Vmax = 11,000 nmol of ATP min-1 (mg of SMP protein)-1 at high energy levels. These data indicate that KmADP and Vmax increase approximately 50-fold each; therefore, there is essentially no change in the catalytic efficiency of the ATP synthase complex in going from one extreme to the other. At intermediate rates of energy production, the kinetic data required introduction of a third, intermediate KmADP. A KmADP of 10-15 microM fitted all the data reported here and previously [Matsuno-Yagi, A., & Hatefi, Y. (1986) J. Biol. Chem. 261, 14031-14038]. However, this is not meant to suggest that there is a fixed intermediate KmADP, as the transition from one fixed limit to the other may be fluid or involve more than one intermediate state. In addition, it has been shown that kinetic plots of SMP-catalyzed and ATP-driven reverse electron transfer from succinate to NAD are curvilinear and resolvable into a minimum of two apparent KmNAD values of about 20-30 and 200-300 microM. These results have been discussed in relation to the three potentially active catalytic sites of F1-ATPase and the structure of the NADH:ubiquinone oxidoreductase complex, the curvilinear kinetics of ATP hydrolysis, and changes in KmADP and KmPi in photophosphorylation as affected by the duration and intensity of light.
Hekman
C
C
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037.
Matsuno-Yagi
A
A
Hatefi
Y
Y
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002417:Cattle; D004579:Electron Transport; D004734:Energy Metabolism; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010085:Oxidative Phosphorylation; D006180:Proton-Translocating ATPases / Q000378:metabolism; D013367:Submitochondrial Particles / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D013386:Succinates; D009243:NAD; D000255:Adenosine Triphosphate; D019802:Succinic Acid; D006180:Proton-Translocating ATPases