Alanine transport systems in isolated basal plasma membrane of human placenta.
Concentrative transfer of amino acids from mother to fetus is affected by transport across both microvillous (maternal-facing) and basal (fetal-facing) plasma membranes of the human placental syncytiotrophoblast. Isolated basal plasma membrane vesicles were used to elucidate transport systems for neutral amino acids across this membrane. The concentration dependence and inhibition of zero-trans-alanine uptake were studied and four pathways for alanine uptake were defined as follows: 1) a sodium-dependent system shared by methylaminoisobutyric acid, which has the characteristics of an A system; 2) a sodium-dependent system resistant to inhibition by methylaminoisobutyric acid, which has the characteristics of an ASC system; 3) a sodium-independent system which may resemble an L system; 4) nonsaturable uptake. The microvillous membrane of the syncytiotrophoblast possesses systems similar to 1 and 3, but system 2 is unique to the basal plasma membrane. Active and passive transport of amino acids across both microvillous and basal plasma membranes may contribute to trophoblast amino acid uptake and nutrition and to the transfer of amino acids to the fetus.
Hoeltzli
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Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, Children's Hospital, St. Louis, Missouri 63110.
Smith
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D000409:Alanine / Q000378:metabolism* / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D000621:Aminoisobutyric Acids / Q000378:metabolism / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism; D004082:Dihydroalprenolol / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy
D000596:Amino Acids; D000621:Aminoisobutyric Acids; C017911:2-(methylamino)isobutyric acid; D004082:Dihydroalprenolol; D003545:Cysteine; D000409:Alanine