title: The penetration of antibiotics into human pancreas.
abstract: In order to analyse the penetration of two antibiotics (mezlocillin and metronidazole) which cover the spectrum of microorganisms involved in pancreatic infection, we determined their concentration in pancreatic tissue, juice and cyst fluid in 16 patients undergoing pancreatic surgery. In addition, the external pancreatic fistula fluid of one patient was analysed for antibiotic concentration and bacterial counts during a seven-day treatment with mezlocillin, metronidazole and netilmicin (i.v.). Antibiotic concentrations were determined by HPLC between 16 and 210 (median 74) min after i.v. administration of 4 g mezlocillin and 500 mg metronidazole, respectively. The median concentration of mezlocillin was 23.2 (range: 3.1-37.4) mg/kg, 15.9 (range: 4.2-55.0) mg/l and 9.9 (range: 5.2-14.8) mg/l in pancreatic tissue, juice and cyst fluid, respectively. The median concentration of metronidazole was 5.1 (range: 1.8-13.0) mg/kg, 8.5 (range: 3.6-16.2) mg/l and 1.2 (0.9-1.4) mg/l in pancreatic tissue, juice and cyst fluid, respectively. From the fistula patient, seven different bacteria were cultured (five aerobic and two anaerobic isolates); their concentration in fistula fluid ranged from 10(5) to 10(7) CFU/ml. The bacteria sensitive for mezlocillin and metronidazole disappeared after four days of i.v. treatment, whereas the two isolates sensitive for netilmicin showed continuous growth seven days after i.v. treatment. The peak concentrations for mezlocillin, metronidazole and netilmicin in the fistula fluid were 6.8 mg/l, 5.6 mg/l and less than 0.1 mg/l, respectively.
authors.affiliation: Department of General Surgery, University of Ulm.
authors.forename: M
authors.initials: M
authors.lastname: Büchler
authors.forename: P
authors.initials: P
authors.lastname: Malfertheiner
authors.forename: H
authors.initials: H
authors.lastname: Friess
authors.forename: R
authors.initials: R
authors.lastname: Bittner
authors.forename: E
authors.initials: E
authors.lastname: Vanek
authors.forename: P
authors.initials: P
authors.lastname: Schlegel
authors.forename: H G
authors.initials: HG
authors.lastname: Beger
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000493:pharmacokinetics* / Q000627:therapeutic use; D008802:Mezlocillin / Q000493:pharmacokinetics* / Q000627:therapeutic use; D008875:Middle Aged; D010179:Pancreas / Q000032:analysis* / Q000601:surgery; D010181:Pancreatic Cyst / Q000378:metabolism; D010185:Pancreatic Fistula / Q000378:metabolism; D010189:Pancreatic Juice / Q000032:analysis; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010195:Pancreatitis / Q000378:metabolism*
chemical_list: D008795:Metronidazole; D008802:Mezlocillin