Evidence for a 2-oxoglutarate dehydrogenase complex activity in mitochondria of Neurospora crassa and compared localization with the pyruvate dehydrogenase complex.
A 2-oxoglutarate dehydrogenase complex activity is demonstrated in Neurospora crassa mitochondria. A submitochondrial fractionation by digitonin treatment followed by freeze-thawing enables measurement of a well preserved activity in the mitochondrial matrix. In contrast to other reports, the pyruvate dehydrogenase activity is also found to be localized in the matrix.
Delattre
P
P
Mareck
A
A
Foucher
B
B
D002458:Cell Fractionation; D003065:Coenzyme A / Q000494:pharmacology; D004072:Digitonin / Q000494:pharmacology; D005615:Freezing; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism*; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure; D009243:NAD / Q000378:metabolism; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D013835:Thiamine Pyrophosphate / Q000494:pharmacology
D011768:Pyruvate Dehydrogenase Complex; D009243:NAD; D007658:Ketone Oxidoreductases; D007655:Ketoglutarate Dehydrogenase Complex; D004072:Digitonin; D013835:Thiamine Pyrophosphate; D003065:Coenzyme A