title: Growth and survival of reovirus in intestinal tissue: role of the L2 and S1 genes.
abstract: Reovirus serotype 1 Lang can be recovered in high titer from the intestines of neonatal mice up to day 8 after peroral inoculation. By contrast, reovirus serotype 3 Dearing cannot be recovered from intestinal tissue past day 4 after peroral inoculation. This difference between the two reoviruses was mapped by using reassortants generated from nonmutagenized laboratory stocks. When the L2 and S1 genes of reovirus serotype 3 Dearing were present in reassortants, the reassortants behaved like serotype 3 Dearing in exhibiting a decreased capacity to be recovered from intestinal tissue. Likewise, viruses which contained the L2 and S2 genes from serotype 1 Lang exhibited an enhanced capacity to grow and survive, which is characteristic of serotype 1 Lang. Thus, the capacity of reovirus to survive in intestinal tissue was determined by the L2 and S1 genes.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Masschusetts.
authors.forename: D K
authors.initials: DK
authors.lastname: Bodkin
authors.forename: B N
authors.initials: BN
authors.lastname: Fields
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology*; D005814:Genes, Viral; D007422:Intestines / Q000382:microbiology*; D051379:Mice; D008815:Mice, Inbred Strains; D012087:Reoviridae / Q000235:genetics / Q000254:growth & development*