Ramoplanin (A-16686), a new glycolipodepsipeptide antibiotic. IV. Complete sequence determination by homonuclear 2D NMR spectroscopy.
Homonuclear 2D NMR spectroscopy double quantum filter correlation spectroscopy (DQF-COSY), relayed-COSY, nuclear Overhauser enhancement spectroscopy (NOESY), and DQF-relayed-NOESY) allowed the complete determination of the core depsipeptide of antibiotic ramoplanin (A-16686). In particular, the DQF-relayed-NOESY experiments were essential in assigning the single signals close to the diagonal.
Kettenring
J K
JK
Merrell Dow Research Institute-Lepetit Research Center, Gerenzano, Italy.
Ciabatti
R
R
Winters
G
G
Tamborini
G
G
Cavalleri
B
B
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides; D006020:Glycopeptides / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000032:analysis; D010456:Peptides, Cyclic / Q000032:analysis; D013057:Spectrum Analysis / Q000379:methods
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D047630:Depsipeptides; D006020:Glycopeptides; D010446:Peptide Fragments; D010456:Peptides, Cyclic; C041438:ramoplanin