title: Structure of T cell antigen receptor beta chain in synovial fluid cells from patients with rheumatoid arthritis.
abstract: We attempted to identify a clonal proliferation of T cells from synovial fluid samples from patients with rheumatoid arthritis, using techniques of restriction fragment length polymorphism. We used probes for the beta chain of the T cell receptor to analyze restriction fragments prepared from the genomic DNA of synovial fluid mononuclear cells from 10 patients and synovial fluid T cell preparations from 5 additional patients. The results demonstrated unarranged (germline) T cell receptor gene fragments of DNA in all cell preparations, indicating the lack of clonality of rheumatoid arthritis synovial fluid T cells.
authors.affiliation: Department of Medicine, Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Canada.
authors.forename: E C
authors.initials: EC
authors.lastname: Keystone
authors.forename: M
authors.initials: M
authors.lastname: Minden
authors.forename: R
authors.initials: R
authors.lastname: Klock
authors.forename: L
authors.initials: L
authors.lastname: Poplonski
authors.forename: J
authors.initials: J
authors.lastname: Zalcberg
authors.forename: T
authors.initials: T
authors.lastname: Takadera
authors.forename: T W
authors.initials: TW
authors.lastname: Mak
mesh_terms: D001172:Arthritis, Rheumatoid / Q000276:immunology*; D002999:Clone Cells; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D013582:Synovial Fluid / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology