title: Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry.
abstract: Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, green/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/green morula cells (MRs), whereas free TC was detected mainly in MRs.
authors.affiliation: Department of Chemistry, Columbia University, New York, New York 10027.
authors.forename: E M
authors.initials: EM
authors.lastname: Oltz
authors.forename: S
authors.initials: S
authors.lastname: Pollack
authors.forename: T
authors.initials: T
authors.lastname: Delohery
authors.forename: M J
authors.initials: MJ
authors.lastname: Smith
authors.forename: M
authors.initials: M
authors.lastname: Ojika
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: Kustin
authors.forename: K
authors.initials: K
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000032:analysis; D002469:Cell Separation; D005434:Flow Cytometry; D009930:Organic Chemicals; D010860:Pigments, Biological / Q000032:analysis*; D014561:Urochordata / Q000032:analysis*; D014639:Vanadium / Q000032:analysis*
chemical_list: D009930:Organic Chemicals; D010860:Pigments, Biological; D014639:Vanadium; C021765:tunichrome