Evidence for the appearance of a reticulocyte population low in lipoxygenase mRNA during the recovery from a phenylhydrazine-induced anemia in rabbits.
It is shown that during recovery from a phenylhydrazine-induced anemia in rabbits a selective decrease in lipoxygenase mRNA takes place with a corresponding shut-off of the synthesis of the enzyme. It is suggested that a new population, 'recovery'-reticulocytes, makes its appearance in the peripheral blood. Their cells are more mature than the stress macroreticulocytes. A cell-free system prepared from the recovery-reticulocytes exhibits low endogenous synthesis of non-globin polypeptides, even without nuclease treatment, but retains full capacity to be stimulated by exogenous mRNA.
Minich
W B
WB
Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region.
Evdokimova
V M
VM
Oleinikov
A V
AV
Höhne
M
M
Thiele
B J
BJ
Rapoport
S M
SM
D000740:Anemia / Q000201:enzymology*; D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000097:blood / Q000235:genetics*; D001091:Arachidonate Lipoxygenases / Q000235:genetics*; D001798:Blood Proteins / Q000096:biosynthesis; D002474:Cell-Free System; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010659:Phenylhydrazines; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000097:blood*; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*
D001798:Blood Proteins; D015342:DNA Probes; D010659:Phenylhydrazines; D012333:RNA, Messenger; C030299:phenylhydrazine; D001091:Arachidonate Lipoxygenases; D001093:Arachidonate 15-Lipoxygenase