title: Interaction between anti-influenza viral polymerase antibodies and RNP particles using the in vitro transcription process and an immunogold labelling technique.
abstract: Immunogold labelling and in vitro transcription of influenza virus vRNA have been used to analyse the interaction of anti-influenza polymerase antibodies with influenza-ribonucleoprotein (RNP) complexes. The polymerase proteins (P proteins) were localized exclusively at one end of the RNP segments. In the course of transcription the amount of P protein decreased significantly. The in vitro transcriptase activity y of influenza A virus RNP complexes in the presence of anti-polymerase antibodies to the strain A/PR/8/34 was inhibited by 60%. In contrast, RNP transcriptase activity of influenza B virus was not inhibited by these antibodies.
authors.affiliation: Department of Molecular Biology, Central Institute of Hygiene, Microbiology and Epidemiology, Berlin, G.D.R.
authors.forename: E
authors.initials: E
authors.lastname: Schreier
authors.forename: A M
authors.initials: AM
authors.lastname: Ladhoff
authors.forename: S
authors.initials: S
authors.lastname: Stompor
authors.forename: S
authors.initials: S
authors.lastname: Michel
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D002642:Chick Embryo; D007150:Immunohistochemistry; D009980:Influenza A virus / Q000254:growth & development / Q000276:immunology*; D008854:Microscopy, Electron; D012316:RNA Nucleotidyltransferases / Q000276:immunology*; D012367:RNA, Viral / Q000096:biosynthesis; D012324:RNA-Dependent RNA Polymerase / Q000276:immunology*; D012261:Ribonucleoproteins / Q000276:immunology*; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal; D012367:RNA, Viral; D012261:Ribonucleoproteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase