title: The nonspecificity of the lead method for the histochemical demonstration of adenosine triphosphatases in human skeletal muscle fibres.
abstract: The lead method for the histochemical demonstration of presumptive mitochondrial adenosinetriphosphatase was applied to biopsy and autopsy samples of the human vastus lateralis muscle. The effect of p-chloromercuribenzoate and of Triton X-100 was tested microdensitometrically and the activity of 'mitochondrial' ATPase was compared to the activity of enzymes of the oxidative metabolism succinic dehydrogenase and NAD-tetrazolium reductase. It is concluded that the ATPase activity displayed is not mainly mitochondrial. In autopsy material, it seems to be predominantly myofibrillar.
authors.forename: I
authors.initials: I
authors.lastname: Erzen
authors.forename: A
authors.initials: A
authors.lastname: Sirca
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000037:antagonists & inhibitors; D000293:Adolescent; D000328:Adult; D001344:Autopsy; D001706:Biopsy; D002729:Chloromercuribenzoates / Q000494:pharmacology; D006651:Histocytochemistry; D006801:Humans; D007854:Lead; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000201:enzymology; D009132:Muscles / Q000033:anatomy & histology / Q000201:enzymology*; D009246:NADH Tetrazolium Reductase / Q000032:analysis; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D013385:Succinate Dehydrogenase / Q000032:analysis; D020245:p-Chloromercuribenzoic Acid
chemical_list: D002729:Chloromercuribenzoates; D007854:Lead; D011092:Polyethylene Glycols; D020245:p-Chloromercuribenzoic Acid; D017830:Octoxynol; D013385:Succinate Dehydrogenase; D009246:NADH Tetrazolium Reductase; D000251:Adenosine Triphosphatases