title: Remarkably AT-rich genomic DNA from the anaerobic fungus Neocallimastix.
abstract: The genomic DNA of an anaerobic rumen phycomycete of the genus Neocallimastix has been purified and characterized. The non-repetitive fraction of the DNA has a G.C content of only 13%. The ribosomal RNA genes are highly reiterated, making up about 30% of the total DNA, and are evident as a more G.C-rich satellite with a repeating unit of about 9.4 kilobases (Kb). A.T-rich regions of DNA are highly dispersed and possess some sequence complexity. Chemical analysis of the DNA constituents reveals no evidence of modified bases. The genome of this anaerobic fungus has the highest A.T content of any organism so far described.
authors.affiliation: CSIRO, Division of Animal Production, Blacktown, NSW, Australia.
authors.forename: A G
authors.initials: AG
authors.lastname: Brownlee
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D008411:Chytridiomycota / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D004276:DNA, Satellite / Q000235:genetics; D008969:Molecular Sequence Data
chemical_list: D004271:DNA, Fungal; D004276:DNA, Satellite