Enhanced induction by high-cholesterol diet of remnant gastric carcinogenesis by N-methyl-N'-nitro-N-nitrosoguanidine in rats.
The influence of bile acids on the development of remnant gastric carcinoma was examined by investigating the incidence of carcinogenesis in noninbred male Wistar rats treated orally with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG; CAS 70-25-7; 1-methyl-3-nitro-1-nitrosoguanidine) and fed a diet containing 1% cholesterol. The high-cholesterol diet did not influence the incidence of carcinoma in the nongastrectomized, MNNG-treated groups of rats. However, in the gastrectomized groups, the incidence of carcinoma was significantly higher in the group given the high-cholesterol diet (60.6%) than in the group given a normal diet (35.5%). Histologically undifferentiated adenocarcinoma was recognized more frequently in the high-cholesterol-diet group. Three gastrectomized rats not treated with MNNG but fed the high-cholesterol diet developed remnant gastric carcinoma (13%), whereas none of the rats given the normal diet did. Because the fecal excretion of bile acids increased significantly in the rats fed the high-cholesterol diet and the gastroduodenal reflux of bile acids was probably accelerated, the increase in the incidence of carcinogenesis in the remnant stomach was considered to be the result of the increase in the reflux of bile acids evoked by a high-cholesterol diet.
Makino
M
M
First Department of Surgery, Tottori University School of Medicine, Yonago, Japan.
Kaibara
N
N
Koga
S
S
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000009:adverse effects*; D003043:Cocarcinogenesis; D005260:Female; D005743:Gastrectomy / Q000009:adverse effects*; D005753:Gastric Mucosa / Q000473:pathology; D008297:Male; D008769:Methylnitronitrosoguanidine; D051381:Rats; D013274:Stomach Neoplasms / Q000209:etiology*
D001647:Bile Acids and Salts; D002791:Cholesterol, Dietary; D008769:Methylnitronitrosoguanidine