A CII-responsive promoter within the Q gene of bacteriophage lambda.
A site within the phage lambda Q gene shares homology with the CII-activated promoters, pE and pI, and is oriented in the direction opposite to that of Q gene transcription. A DNA fragment containing this site can serve as a template for CII-activated transcription in vitro. To ask if this presumptive CII control site functions as a CII-activated promoter in vivo, a restriction fragment containing this promoter has been cloned on a plasmid so that synthesis of beta-galactosidase will be under its control. When CII protein is supplied in trans from a compatible plasmid, this promoter, designated PaQ, is activated to produce beta-galactosidase. A promoter positioned within the Q gene which can be activated by CII protein to initiate transcription in an anti-sense direction should result in an interference with Q gene expression, enhancing CII regulation of late functions, and adding to the list of known CII controls on the lysogenic response.
Stephenson
F H
FH
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D005786:Gene Expression Regulation; D005814:Genes, Viral; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
D014157:Transcription Factors; D001616:beta-Galactosidase