Electron probe micro-analysis for subsurface demineralization and remineralization of dental enamel.
A quantitative study of fluoride distribution profile changes in dental enamel was conducted by means of electron probe micro-analysis (EPMA). Fluoride-deposited hydroxyapatite powders were chosen as fluoride standards, and analytical conditions were optimized. The lower limit of detection for fluoride was estimated to be 270 ppm, with an accelerating voltage of 5 kV, a specimen current of 40 nA, and a counting time of 40 seconds. Fluoride profiles in fluoride-treated dental enamel, which exhibited intact surface layers and subsurface demineralization, were determined. The results were also compared with those of an acid-abrasion method, and reasonable consistency was found between these two methods, although the acid-abrasion procedure yielded a slightly lower fluoride content in the initial layers, followed by a higher content of fluoride in the deeper layers. The precision of fluoride profile data obtained from EPMA permits further studies to be conducted on the kinetics of subsurface demineralization and intact surface layer formation ("white spot" formation) which is observed during the acid challenge of dental enamel.
Chu
J S
JS
Department of Pharmaceutics, University of Utah, Salt Lake City 84112.
Fox
J L
JL
Higuchi
W I
WI
Nash
W P
WP
D000818:Animals; D002417:Cattle; D003731:Dental Caries / Q000378:metabolism*; D003743:Dental Enamel / Q000032:analysis* / Q000378:metabolism; D004577:Electron Probe Microanalysis / Q000379:methods; D005459:Fluorides / Q000493:pharmacokinetics*; D014089:Tooth Remineralization
D005459:Fluorides