title: Axolinin localization in the nervous tissue of squid revealed by monoclonal antibodies specific for axolinin: cellular and subcellular localization of axolinin in the squid neuron.
abstract: Cellular and subcellular distributions of axolinin, the 260-kilodalton (kD) microtubule-associated glycoprotein originally purified from squid axons, in various squid tissues such as optical lobes, bundles of small nerve fibers (fin nerves), giant stellate ganglia, skin, muscle, liver, and gill, were immunologically studied using monoclonal antibodies specifically recognizing the polypeptide chain of axolinin. The following results were obtained: (1) Axolinin is confined to squid neurons and skin; (2) axolinin is localized in the axon whereas another 260-kD microtubule-associated protein, MAP B, is localized in the cell bodies; and (3) axolinin is localized mainly in the peripheral part of the axoplasm of the squid giant axon. The last result has confirmed our previous conclusion obtained using polyclonal antisera against axolinin, which contain antibodies recognizing not only axolinin-specific epitopes but also nonspecific epitopes. The physiological importance of the localization of axolinin in axons and the skin is discussed based on its possible relationship to excitability function.
authors.affiliation: Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Arai
authors.forename: G
authors.initials: G
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D049832:Decapodiformes; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000032:analysis*; D012867:Skin / Q000032:analysis; D013347:Subcellular Fractions / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C048691:axolinin