Isoenzyme patterns of protein kinase C and a phospholipid-dependent but Ca2+-inhibited enzyme fraction in the crude extracts of different tissues.
We compared the protein kinase C isoenzyme patterns of crude extracts of rabbit brain, cerebellum, spleen, thymus and human and pig granulocytes. The isoenzymes were fractionated by hydroxyapatite chromatography and the protein kinase C activity was determined with a synthetic oligopeptide substrate. In the extracts of several tissues we also observed an enzyme fraction which was activated by phosphatidylserine + diacylglycerol but inhibited by Ca2+.
Faragó
A
A
1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Farkas
G
G
Mészáros
G
G
Buday
L
L
Antoni
F
F
Seprödi
J
J
D000818:Animals; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D002845:Chromatography / Q000379:methods; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000037:antagonists & inhibitors; D010718:Phosphatidylserines / Q000494:pharmacology; D011493:Protein Kinase C / Q000032:analysis* / Q000037:antagonists & inhibitors; D011817:Rabbits; D013154:Spleen / Q000201:enzymology; D013552:Swine; D013950:Thymus Gland / Q000201:enzymology; D014020:Tissue Extracts / Q000032:analysis
D004075:Diglycerides; D007527:Isoenzymes; D010718:Phosphatidylserines; D014020:Tissue Extracts; D011493:Protein Kinase C; D002118:Calcium