title: Caprine acrosin. Purification, characterization and proteolysis of the porcine zona pellucida.
abstract: Acrosin purified from an acidic extract of ejaculated goat spermatozoa migrated as a single 42,000-Mr band in SDS/polyacrylamide-gel electrophoresis. Reduction and alkylation of caprine acrosin produced two polypeptides, one of Mr 40,000 (heavy chain) and the other of Mr 3700 (light chain). The light chain purified by reversed-phase h.p.l.c. was a glycosylated octadecapeptide with an amino acid sequence similar to that of the N-terminal 18 residues of porcine acrosin light chain (78% positional identity). The sequence of the N-terminal 37 amino acids of purified caprine acrosin heavy chain is similar to that of porcine acrosin heavy chain (70% positional identity through 37 residues). Studies with synthetic substrates and synthetic and natural proteinase inhibitors confirmed both the specificity of the purified proteinase for Arg-Xaa and Lys-Xaa bonds and a serine-proteinase mechanism. Purified caprine acrosin hydrolysed the 90 kDa and 65 kDa components, but did not hydrolyse the 55 kDa component of the porcine zona pellucida. The action of the enzyme on the porcine zona pellucida was indistinguishable from that previously reported for porcine acrosin.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, Davis 95616.
authors.forename: D M
authors.initials: DM
authors.lastname: Hardy
authors.forename: A F
authors.initials: AF
authors.lastname: Schoots
authors.forename: J L
authors.initials: JL
authors.lastname: Hedrick
mesh_terms: D000176:Acrosin / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D012697:Serine Endopeptidases / Q000302:isolation & purification*; D013552:Swine; D015044:Zona Pellucida / Q000378:metabolism
chemical_list: D010455:Peptides; D012697:Serine Endopeptidases; D000176:Acrosin