title: [Detection of dipeptidyl peptidase IV in follicular fluid--initial report].
abstract: Aspirates of ovarian follicles were obtained from patients subjected to gynaecologic surgeries. This is the first report of dipeptidyl-peptidase IV (DP IV) activity in human follicular fluid. This enzyme specifically cleaves regulatory important oligo- and polypeptides. Only from peptides with proline or alanine in position P1 of the N-terminal sequence a dipeptide is split off. DP IV activity was measured with glycyl-L-proline p-Nitroanilide as substrate. Levels in a range of 209.8 +/- 57.4 nmol l-1 s-1 were achieved.
authors.affiliation: Klinik und Poliklinik für Gynäkologie und Geburtshilfe, Martin-Luther-Universität Halle-Wittenberg.
authors.forename: E
authors.initials: E
authors.lastname: Seliger
authors.forename: V
authors.initials: V
authors.lastname: Blumenauer
authors.forename: J
authors.initials: J
authors.lastname: Heins
authors.forename: K
authors.initials: K
authors.lastname: Neubert
authors.forename: P
authors.initials: P
authors.lastname: Kaltwasser
authors.forename: C
authors.initials: C
authors.lastname: Schöneich
authors.forename: R
authors.initials: R
authors.lastname: Bergleiter
mesh_terms: D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism*; D005260:Female; D006801:Humans; D006080:Ovarian Follicle / Q000201:enzymology*; D010455:Peptides / Q000378:metabolism
chemical_list: D010455:Peptides; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4
vernacular_title: Der Nachweis der Dipeptidyl-Peptidase IV in der Follikelflüssigkeit--ein erster Bericht.