On thymidine incorporation by the cultured chick pineal gland.
Explanted chick pineal glands exhibited a cycle in thymidine incorporation when cultured either under a cycle of illumination or in constant darkness. This cycle appeared to be entrained to the light cycle under which the birds were maintained. The incorporation reflected gene replication in a small fraction of the cell population that was largely, but not entirely, located in the stroma of the gland. Glands cultured with colchicine for 28 h contained a very small number of cells showing metaphase chromosomes.
Wainwright
S D
SD
Biochemistry Department, Dalhousie University, Halifax, N.S., Canada.
Wainwright
L K
LK
Lucarotti
C J
CJ
D000818:Animals; D002645:Chickens; D002940:Circadian Rhythm; D003078:Colchicine / Q000494:pharmacology; D004261:DNA Replication; D008027:Light; D009924:Organ Culture Techniques; D010870:Pineal Gland / Q000187:drug effects / Q000378:metabolism*; D013936:Thymidine / Q000493:pharmacokinetics*
D003078:Colchicine; D013936:Thymidine