Cloning and sequence of a secretory protein induced by growth factors in mouse fibroblasts.
From a collection of 80 cDNAs representing genes that are induced by serum growth factors in quiescent mouse fibroblasts we have characterized a clone, N51, and demonstrated that it codes for a precursor of a secretory protein. N51 mRNA is rapidly induced by serum, including in the presence of cycloheximide, demonstrating that its induction does not require de novo protein synthesis. The mRNA has a short half-life of approximately 10-15 min and is dramatically prolonged in the presence of protein synthesis inhibitors. Purified growth factors such as platelet-derived growth factor and bombesin are strong inducers of N51 expression. However, epidermal growth factor, fibroblast growth factor, and the tumor promoter tetradecanoyl phorbol acetate have a negligible effect. Transcriptional analysis demonstrates that the increase in N51 mRNA levels after serum stimulation is in part due to the activation of gene transcription.
Ryseck
R P
RP
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
MacDonald-Bravo
H
H
Mattei
M G
MG
Bravo
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001769:Blood; D001839:Bombesin / Q000494:pharmacology; D002460:Cell Line; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003470:Culture Media; D004247:DNA; D005786:Gene Expression Regulation / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D007399:Interphase; D051379:Mice; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D003470:Culture Media; C475895:Cxcl1 protein, mouse; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor; D011498:Protein Precursors; D012333:RNA, Messenger; D004247:DNA; D001839:Bombesin