Organization and operation of a flow cytometric immunophenotyping laboratory.
Technical advances in the field of flow cytometry have made it feasible for many academic and private hospital laboratories to purchase relatively inexpensive "user friendly" flow cytometers that do not require dedicated flow cytometer operators, special rooms, or a significant amount of laboratory space. Because the financial and physical constraints in operating a flow cytometer have been substantially reduced, many pathologists may now be considering such a purchase. By chronicling the clinical activities of a single flow cytometric immunophenotyping laboratory, this report will answer a variety of questions that may be asked by pathologists regarding both the utility of flow cytometers in clinical diagnosis and the mechanics of operating an immunophenotyping laboratory. The types of tissues that can be evaluated by flow cytometry will be detailed, and we will summarize the number and type of flow cytometric clinical studies performed in our laboratory since its development in 1983. Practical aspects of laboratory operation including technical staff requirements, specimen handling and processing procedures, monoclonal antibody selection, and quality control procedures will be presented. In addition, a comprehensive review of flow cytometric immunophenotyping studies as applied to the diagnosis of leukemias, lymphomas, and immunodeficiency disorders will be presented along with case examples that illustrate our approach to the interpretation of immunophenotyping results.
Foucar
K
K
University of New Mexico, School of Medicine, Department of Pathology, Albuquerque 87131.
Chen
I M
IM
Crago
S
S
D000328:Adult; D000911:Antibodies, Monoclonal; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007159:Immunologic Tests; D007755:Laboratories, Hospital / Q000458:organization & administration* / Q000592:standards; D007938:Leukemia / Q000175:diagnosis; D008223:Lymphoma / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011785:Quality Assurance, Health Care; D011786:Quality Control
D000911:Antibodies, Monoclonal