Influence of ochratoxin B on the ochratoxin A inhibition of phenylalanyl-tRNA formation in vitro and protein synthesis in hepatoma tissue culture cells.
Ochratoxin B (OTB), the dechloro-analogue of ochratoxin A (OTA), was studied separately and in combination with OTA on the aminoacylation of phenylalanine tRNA (tRNAPhe) catalysed by mice liver phenylalanyl-tRNA synthetase. OTB was neither a significant inhibitor of the reaction nor an antagonist of OTA. OTB was also assayed for its possible antagonistic effect on the in vivo protein synthesis inhibition caused by OTA in hepatoma tissue culture cells. No prevention of OTA inhibition could be found for OTB. It rather showed a slight additional inhibitory activity when mixed (100-180 microM) with low concentrations of OTA (40-60 microM). In conclusion, these results are not in favor of an antagonistic effect of OTB with respect to OTA action, at least on the level of cellular protein synthesis.
Roth
A
A
Institut de Biologie Moléculaire et Cellulaire du CNRS, Université Louis Pasteur, Strasbourg, France.
Creppy
E E
EE
Kane
A
A
Bacha
H
H
Steyn
P S
PS
Röschenthaler
R
R
Dirheimer
G
G
D000818:Animals; D007930:Leucine / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D009793:Ochratoxins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012345:RNA, Transfer, Amino Acid-Specific / Q000096:biosynthesis*; D012360:RNA, Transfer, Phe / Q000096:biosynthesis*; D051381:Rats; D014407:Tumor Cells, Cultured
D009793:Ochratoxins; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; C025589:ochratoxin A; C045354:ochratoxin B; D007930:Leucine