Isolation of the Xenopus homolog of int-1/wingless and expression during neurula stages of early development.
We have isolated the Xenopus homolog (Xint-1) of the mouse protooncogene int-1 from a neurula stage 17 cDNA library. The deduced protein sequence of Xint-1 includes 371 amino acids. The Xint-1 protein is more similar to the mammalian int-1 product (69%), than to the Drosophila counterpart of int-1, wingless (50%). Xint-1 shares several characteristics of secreted proteins with the other int-1 homologs: it has a hydrophobic leader, multiple conserved potential N-linked glycosylation sites and is rich in cysteine residues. All 23 cysteines are conserved in the three proteins. Xint-1 is transiently expressed during the neurula stages of early Xenopus development.
Noordermeer
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Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
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Verrijzer
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Rijsewijk
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D005796:Genes; D051379:Mice; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology*; D011519:Proto-Oncogenes; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*
D004247:DNA