A rapid and efficient purification of poly(A)-mRNA by oligo(dT)30-Latex.
Latex particles were covalently linked to the 5'-proximal region of oligo(dT)30. The resultant oligo(dT)30-Latex was tested for its hybridizability to poly(A) containing mRNA. Several advantages were noted as compared to the conventional oligo(dT)30-cellulose column chromatography; (1) a highly efficient (approximately 95%) hybridization occurs in a short reaction period (10min), (2) more than 95% of poly(A) mRNA can be recovered from oligo(dT)30-Latex by a simple heating followed by brief centrifugation, (3) multiple samples can be handled simultaneously and moreover, (4) the poly(A)-mRNA on the oligo(dT)30-Latex can be directly transcribed by AMV reverse transcriptase to form the cDNA. These properties of oligo(dT)30-Latex promise an excellent reagent for nucleic acid technology.
Kuribayashi
K
K
Japan Synthetic Rubber Co., Ltd., Tokyo Research Laboratory, Kawasaki, Japan.
Hikata
M
M
Hiraoka
O
O
Miyamoto
C
C
Furuichi
Y
Y
D002846:Chromatography, Affinity / Q000379:methods; D004279:DNA, Viral / Q000235:genetics; D007202:Indicators and Reagents; D007840:Latex; D009838:Oligodeoxyribonucleotides; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D014616:Vaccinia virus / Q000235:genetics
D004279:DNA, Viral; D007202:Indicators and Reagents; D007840:Latex; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C027903:oligo (dT); D011061:Poly A