Identification of 20 alpha-hydroxy-5 alpha-pregnan-3-one and 20 beta-hydroxy-5 alpha-pregnan-3-one in human pregnancy urine.
20 beta-Hydroxy-5 alpha-pregnan-3-one and 20 alpha-hydroxy-5 alpha-pregnan-3-one were isolated and identified from a pool of urine collected from women in the third trimester of pregnancy. Following isolation by Sephadex LH-20 and HPLC, the identity of each compound was established by comparison of GC-MS data for the methyloxime-trimethylsilyl ethers with those for authentic standards.
Philip
A
A
Reproductive Physiology Unit, Montreal General Hospital, Canada.
Pike
A W
AW
Fennessey
P V
PV
Murphy
B E
BE
D002851:Chromatography, High Pressure Liquid; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011247:Pregnancy / Q000652:urine*; D011278:Pregnanes / Q000652:urine*; D011280:Pregnanolone / Q000652:urine*; D012016:Reference Values
D011278:Pregnanes; D011280:Pregnanolone