title: Species pattern of phosphatidic acid, diacylglycerol, CDP-diacylglycerol and phosphatidylglycerol synthesized de novo in rat liver mitochondria.
abstract: Rat liver mitochondria were incubated with [3H]glycerol 3-phosphate, ATP, CTP and coenzyme A allowing acylatin of glycerophosphate with endogenous fatty acids and the further conversion of labelled phosphatidic acid (PA) to diacylglycerol (DG), CDP-diacylglycerol (CDP-DG) and phosphatidylglycerol (PG). In these glycerolipids, the distribution of label among the individual molecular species was found to be similar, with 16:0-18:1, 16:0-18:2 and 18:0-18:2/16:0-16:0 being the main species. It was concluded that mitochondrial enzymes involved in the de novo synthesis of these glycerolipids exhibited no acyl selectivity for their substrates. The pattern of molecular species of mitochondrial PA, DG and CDP-DG closely approached that of the same glycerolipids synthesized de novo in isolated rat liver microsomes.
authors.affiliation: Institute of Pathological and Clinical Biochemistry, Charite Hospital, Humboldt University, Berlin, G.D.R.
authors.forename: B
authors.initials: B
authors.lastname: Rüstow
authors.forename: M
authors.initials: M
authors.lastname: Schlame
authors.forename: H
authors.initials: H
authors.lastname: Rabe
authors.forename: G
authors.initials: G
authors.lastname: Reichmann
authors.forename: D
authors.initials: D
authors.lastname: Kunze
mesh_terms: D000818:Animals; D003567:Cytidine Diphosphate Diglycerides / Q000096:biosynthesis*; D004075:Diglycerides / Q000096:biosynthesis*; D005989:Glycerides / Q000096:biosynthesis*; D005994:Glycerophosphates / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology*; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D010712:Phosphatidic Acids / Q000096:biosynthesis*; D010715:Phosphatidylglycerols / Q000096:biosynthesis*; D051381:Rats
chemical_list: D003567:Cytidine Diphosphate Diglycerides; D004075:Diglycerides; D005989:Glycerides; D005994:Glycerophosphates; D009702:Nucleoside Diphosphate Sugars; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; C029620:alpha-glycerophosphoric acid