Evaluation of dust respirators for elimination of mouse aeroallergens.
The efficiency of various dust respirators for eliminating mouse allergens [mouse urine proteins (MUP), pelts proteins (MPP) and serum albumin (MSA)] were evaluated with use of low-volume air samplers and immunochemical methods. Three kinds of dust respirators from one manufacturer which have different efficacy in the exclusion of dust particles were put on the fiber glass filter in each air sampler. Then the air in a mouse housing room was sampled. The allergens passed through the respirators, were trapped in the fiber glass filters, and then extracted from the filters. The allergens of MUP and MPP in the extract were measured by an inhibition method of fluorometric enzyme-linked immunosorbent assay (ELISA) for IgE antibody and those of MSA measured by a fluorometric sandwich ELISA. The respirator with the lowest capability of exclusion was found to eliminate 65-86% of respective allergens. The other two respirators with higher powers eliminated 98% of MUP. MPP and MSA were eliminated to undetectable levels through these respirators. This study provided a means for the evaluation of dust respirators for animal aeroallergens.
Sakaguchi
M
M
Department of Microbiology, Institute of Public Health, Tokyo, Japan.
Inouye
S
S
Miyazawa
H
H
Kamimura
H
H
Kimura
M
M
Yamazaki
S
S
D000397:Air Pollution; D000485:Allergens; D000818:Animals; D004391:Dust; D004797:Enzyme-Linked Immunosorbent Assay; D006197:Hair; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011482:Protective Devices / Q000662:veterinary*; D012134:Respiratory Protective Devices / Q000592:standards / Q000662:veterinary*; D014556:Urine
D000485:Allergens; D004391:Dust