Schistosoma mansoni: pairing of male worms with artificial surrogate females.
To determine whether male worms provide any specific polypeptides to females, we produced extruded alginate fibers that resembled the size and shape of mature female worms. Males clasped these surrogate "female worms" and remained "paired" with them for long periods. On polyacrylamide gel electrophoresis, fibers clasped for several days showed bands at approximately 40 and 46 kDa which were never found in fibers incubated in the same medium but not clasped by males. We believe that these may be substances transferred from male worms during normal pairing. Males biosynthetically labeled with [14C]leucine were permitted to pair with fibers, which took up a broad range of polypeptides visualized on long autoradiographic exposure to gels.
Basch
P F
PF
Department of Health Research and Policy, Stanford University School of Medicine, California 94305.
Nicolas
C
C
D000464:Alginates / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008297:Male; D010455:Peptides / Q000032:analysis; D012550:Schistosoma mansoni / Q000032:analysis / Q000502:physiology*
D000464:Alginates; D006603:Hexuronic Acids; D010455:Peptides; D020723:Glucuronic Acid