Differential expression of influenza N protein and neuraminidase antigenic determinants in Escherichia coli.
Two influenza gene products of similar size and codon usage have been expressed in Escherichia coli under control of the phage lambda pR promoter. The influenza N protein (NP) was expressed in its entirety after fusion to a short (12 amino acid) segment of the lambda cro gene product and constituted about 1-2% of total soluble cell protein after induction. By contrast, constructions using the full length neuraminidase (NA) gene failed to give rise to detectable amounts of NA antigen after fusion to either the 12 amino acid Cro peptide or after fusion to bacterial beta-galactosidase (beta gal). Rather, expression of NA antigenic determinants was only achieved after deletion of coding sequences at the 3' end of the beta gal-NA fusion construct such that the encoded protein precipitated within the cell.
Jones
I M
IM
Brownlee
G G
GG
D000956:Antigens, Viral / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D008970:Molecular Weight; D009439:Neuraminidase / Q000235:genetics* / Q000276:immunology; D010447:Peptide Hydrolases / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014764:Viral Proteins / Q000235:genetics*
D000956:Antigens, Viral; D004274:DNA, Recombinant; D014764:Viral Proteins; D009439:Neuraminidase; D010447:Peptide Hydrolases