Incorporation and disappearance of oxygen-18 in lung from mice exposed to 1 ppm 18O3.
In this study, 18O3 was used as a tracer for inhaled ozone in mice. The amount of ozone-derived oxygen (ODO) in the lungs was determined by measuring the amount of oxygen-18 in excess of the natural abundance level which remained covalently bound to organic constituents of lung following exposure to 1 ppm 18O3 for less than or equal to 60 min. The rate of disappearance of ODO from the lungs was determined by quantifying the rate of decrease of oxygen-18 in excess of the natural abundance level in lung from mice exposed to 1 ppm 18O3 for 45 min. With exposure to 1 ppm 18O3. ODO accumulated in lung at a rate of 4.38 pmol/mg dry weight/min. Ozone-derived oxygen had a half-life in lung of approximately 6 hr. We estimate that a minimum of 44 pmol of O3 reacted with lung every minute of exposure to 1 ppm O3.
Santrock
J
J
Department of Chemistry, Indiana University, Bloomington 47405.
Hatch
G E
GE
Slade
R
R
Hayes
J M
JM
D000818:Animals; D001273:Atmosphere Exposure Chambers; D005260:Female; D008168:Lung / Q000378:metabolism*; D051379:Mice; D010100:Oxygen / Q000378:metabolism* / Q000493:pharmacokinetics; D010103:Oxygen Isotopes; D010126:Ozone / Q000008:administration & dosage / Q000378:metabolism*; D013997:Time Factors
D010103:Oxygen Isotopes; D010126:Ozone; D010100:Oxygen