Regulation of colony formation of differentiated chondrocytes in soft agar by transforming growth factor-beta.
Fibroblast growth factor (FGF) induces colony formation by chondrocytes in soft agar (Y. Kato et al., J. Cell. Physiol., 1987), and the present study revealed that transforming growth factor-beta(TGF-beta) does not induce the same effect. TGF-beta did, however, increase the efficiency of colony formation by chondrocytes 3- to 4-fold in the presence of a maximal dose of FGF. Furthermore, TGF-beta decreased the concentrations of FGF needed for the induction of cell growth in soft agar by 40- to 100-fold. These results suggest that TGF-beta is involved in the control of cartilage growth possibly by increasing the responsiveness of chondrocytes to FGF.
Iwamoto
M
M
Department of Biochemistry, Faculty of Denistry, Osaka University, Japan.
Sato
K
K
Nakashima
K
K
Fuchihata
H
H
Suzuki
F
F
Kato
Y
Y
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003114:Colony-Forming Units Assay; D004247:DNA / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D007700:Kinetics; D011817:Rabbits; D015291:Transforming Growth Factors / Q000494:pharmacology*
D005346:Fibroblast Growth Factors; D015291:Transforming Growth Factors; D003094:Collagen; D004247:DNA