Reperfusion injury in the lung preserved for 24 hours.
The left lower lobes of 28 canine lungs were isolated, preserved, and then reperfused for 150 minutes. Five groups of lobes were studied: group 1, control (n = 5); group 2, one hour of warm ischemia (n = 5); group 3, one hour of warm ischemia + oxygen free radical scavengers (n = 5); group 4, 24 hours of cold ischemia (n = 8); and group 5, 24 hours of cold ischemia + oxygen free radical scavengers (n = 5). Oxygen free radical scavengers consisted of superoxide dismutase and catalase (100 micrograms/mL) given at the moment of reflow. Extravascular lung water (grams per gram of blood-free dry lobe weight) after reperfusion was 2.75 +/- 0.19, 5.46 +/- 0.60, 4.08 +/- 0.37, 9.43 +/- 0.98, and 6.91 +/- 0.95 for groups 1 through 5, respectively (p less than 0.05, groups 2 through 5 versus group 1; p less than 0.05, group 2 versus group 3 and group 4 versus group 5). Lung tissue lipid peroxidation, measured as thiobarbituric acid reactive material, was 117 +/- 14, 314 +/- 19, and 163 +/- 25 nmol/g dry lobe weight for groups 1, 4, and 5, respectively (p less than 0.05, group 4 versus group 1 and group 4 versus group 5). The data suggest that oxygen free radical scavengers attenuate reperfusion injury after long-term hypothermic lung preservation.
Paull
D E
DE
Division of Cardiothoracic Surgery, University of North Carolina School of Medicine, Chapel Hill 27599-7065.
Keagy
B A
BA
Kron
E J
EJ
Wilcox
B R
BR
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D004285:Dogs; D005609:Free Radicals; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D009926:Organ Preservation / Q000379:methods; D009929:Organ Size / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D011652:Pulmonary Circulation / Q000187:drug effects; D011654:Pulmonary Edema / Q000209:etiology / Q000473:pathology; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013997:Time Factors
D005609:Free Radicals; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen