Comparison of synexin isotypes in secretory and non-secretory tissues.
Three synexin isotypes were identified in bovine liver or adrenal medullary tissues by immune blotting of one- or two-dimensional SDS gels and by two-dimensional tryptic peptide mapping of gel bands or spots. These isotypes were: alpha-synexin, mass 47 kDa, pI 6.9; beta-synexin, mass 47 kDa, pI 6.5; and mu-synexin, mass 51 kDa, pI 6.1. A non-secretory tissue, bovine skeletal muscle, was found to contain only mu-synexin. The absence of alpha- and beta-synexins in a non-secretory tissue suggests these proteins may perform specific roles in the process of exocytosis.
Dowling
L G
LG
Creutz
C E
CE
D000313:Adrenal Medulla / Q000032:analysis*; D000818:Animals; D017310:Annexin A7; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002837:Chromaffin Granules / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D007163:Immunosorbent Techniques; D007526:Isoelectric Point; D008099:Liver / Q000032:analysis*; D009132:Muscles / Q000032:analysis*; D009391:Nephelometry and Turbidimetry; D011506:Proteins / Q000032:analysis* / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism
D017310:Annexin A7; D011506:Proteins; D014357:Trypsin; D002118:Calcium