Involvement of perivascular neuropeptide Y nerve fibres in uterine arterial vasoconstriction in conjunction with pregnancy.
The perivascular neuropeptide Y (NPY) innervation and its relation to adrenergic nerves of uterine arteries from non-pregnant and pregnant guinea pigs was analyzed immunocytochemically. The NPY content of the uterine artery was, in addition, measured radioimmunologically (RIA). Vasomotor effects of NPY per se and in combination with other vasoconstrictors were examined using a sensitive in vitro method. Pregnancy did not visibly affect density and distribution of NPY-immunoreactive fibres. The NPY fibres contained in addition immunoreactivity to dopamine-beta-hydroxylase (marker for noradrenergic neurons). RIA revealed a slight decrease of NPY content during pregnancy, probably due to the increased smooth muscle volume of uterine arteries. The contractile effect of NPY on uterine arteries was weak, while vasoconstriction induced by various agonists was potentiated by NPY, particularly during pregnancy. It is concluded that perivascular NPY-containing nerve fibres may be involved in the dramatic blood flow alterations that occur in the uterine circulation in connection with pregnancy and partus.
Fallgren
B
B
Department of Zoophysiology, University of Lund, Sweden.
Edvinsson
L
L
Ekblad
E
E
Ekman
R
R
D000818:Animals; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009412:Nerve Fibers / Q000502:physiology*; D009478:Neuropeptide Y / Q000502:physiology*; D011247:Pregnancy; D011863:Radioimmunoassay; D014599:Uterus / Q000098:blood supply* / Q000294:innervation; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D009478:Neuropeptide Y; D014662:Vasoconstrictor Agents