Active and latent forms of transforming growth factor beta activity in synovial effusions.
We have evaluated the possible involvement of TGF-beta in rheumatoid arthritis by assay of 16 cell-free synovial fluids for the presence of its active and "latent" forms. Evidence has been obtained for TGF-beta-like activity in synovial effusions by four criteria: (a) TGF-beta receptor competition, (b) soft-agar colony formation of AKR-2B and NRK-49F indicator cells, (c) immunological neutralization of the biological activity, and (d) biochemical activation of a latent form.
Fava
R
R
Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Olsen
N
N
Keski-Oja
J
J
Moses
H
H
Pincus
T
T
D000937:Antigen-Antibody Reactions; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D001792:Blood Platelets / Q000502:physiology; D006801:Humans; D007717:Knee; D011869:Radioligand Assay; D013582:Synovial Fluid / Q000378:metabolism*; D015291:Transforming Growth Factors / Q000378:metabolism*
D015291:Transforming Growth Factors