Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants.
In this report, we provide a new method for selection of vaccinia virus recombinants expressing foreign genes. The method is based on the use of the gene encoding the viral 14,000-molecular-weight envelope protein that rescues the small-plaque-size phenotype of a vaccinia virus variant to large-plaque-size virus. Selection of recombinants is easily obtained after visual inspection of large viral plaques.
Rodriguez
J F
JF
Department of Biochemistry, Health Science Center, State University of New York, Brooklyn 11203.
Esteban
M
M
D005819:Genetic Markers; D010641:Phenotype; D011995:Recombination, Genetic; D012641:Selection, Genetic; D014616:Vaccinia virus / Q000235:genetics* / Q000502:physiology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000502:physiology; D010948:Viral Plaque Assay
D005819:Genetic Markers; D014759:Viral Envelope Proteins