title: The purification of an erythroid protein which binds to enhancer and promoter elements of haemoglobin genes.
abstract: An erythroid nuclear protein (EF1), originally detected as a protein binding within the nuclease hypersensitive site upstream of the chicken beta H-globin gene, has been purified. This protein of 37,000-39,000 molecular weight binds to three sites within the hypersensitive region: one between the CCAAT and TATA boxes, the second (further upstream) next to a NF1 binding site, and the third adjacent to a regulatory element found in a number of beta-globin genes. The EF1 protein also binds to an erythroid-specific promoter element of the mouse alpha-globin gene and to two sites within the chicken beta A-globin enhancer. These six EF1-binding sites are related by the consensus sequence A/TGATAA/GG/C. A minor protein of molecular weight 72,000 which co-purifies with EF1 also binds to the same sequences.
authors.affiliation: Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
authors.forename: N D
authors.initials: ND
authors.lastname: Perkins
authors.forename: R H
authors.initials: RH
authors.lastname: Nicolas
authors.forename: M A
authors.initials: MA
authors.lastname: Plumb
authors.forename: G H
authors.initials: GH
authors.lastname: Goodwin
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002645:Chickens; D002846:Chromatography, Affinity; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D004912:Erythrocytes / Q000378:metabolism; D005796:Genes; D005914:Globins / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000097:blood*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
chemical_list: D009687:Nuclear Proteins; D005914:Globins; D003850:Deoxyribonuclease I