Growth hormone stimulates insulin-like growth factor I actions on adult articular chondrocytes.
We report effects of adding insulin-like growth factor I (IGF-I) and methionyl human growth hormone (GH), alone or in combination, to adult bovine articular chondrocytes plated at high density. Purified human and synthetic IGF-I stimulated chondrocyte DNA and proteoglycan synthesis. GH had no effect on either process. However, GH added in combination with IGF-I increased proteoglycan, cell-associated proteoglycan, and keratan sulfate synthesis over levels observed with IGF-I alone. IGF-I and GH did not alter the hydrodynamic size of proteoglycans or synthesis of collagen. Our results show that GH and IGF-I act together to stimulate adult chondrocyte extracellular matrix synthesis.
Smith
R L
RL
Orthopedic Research Laboratory, Stanford University School of Medicine.
Palathumpat
M V
MV
Ku
C W
CW
Hintz
R L
RL
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000096:biosynthesis; D013006:Growth Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D019382:Human Growth Hormone; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007632:Keratan Sulfate / Q000096:biosynthesis; D011392:Proline / Q000378:metabolism; D011509:Proteoglycans / Q000096:biosynthesis; D013002:Somatomedins / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D006025:Glycosaminoglycans; D006728:Hormones; D011509:Proteoglycans; D013002:Somatomedins; D019382:Human Growth Hormone; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D004247:DNA; D007632:Keratan Sulfate; D011392:Proline; D013936:Thymidine