A three-dimensional reconstruction of high endothelial venules in the mouse lymph node: an enzyme-histochemical study.
A three-dimensional reconstruction of high endothelial venules (HE-venules) of an entire mouse lymph node is presented. The reconstruction has been made by means of the histochemical technique for alpha-naphthylacetate esterase. The course of the HE-venules is shown in coherence with lymphocytic aggregates (follicles and unit), which were concomitantly reconstructed. Carbonic anhydrase, glutamate-, and alpha-glycerophosphate dehydrogenase were found in the high endothelia, while calcium-stimulated NA+, K+ ATP-ase and the acetylcholinesterase were localized to the endothelia and/or to the perivascular sheath of the HE-venules and submarginal capillaries.
Müller
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E
Stempfel
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D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D004727:Endothelium / Q000033:anatomy & histology / Q000201:enzymology; D006651:Histocytochemistry; D006801:Humans; D008198:Lymph Nodes / Q000033:anatomy & histology* / Q000201:enzymology; D051379:Mice; D008815:Mice, Inbred Strains; D009283:Naphthol AS D Esterase / Q000378:metabolism
D009283:Naphthol AS D Esterase; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases