Clonal selection by fluorescence redistribution after photobleaching (FRAP)--a "fast" lateral mobility fibroblast mutant (E7G1).
Fluorescence redistribution after photobleaching (FRAP) was utilized to select a "fast" lateral mobility clone from Kirsten murine sarcoma virus-transformed 3T3 (KMSV-3T3) fibroblasts. The clone, E7G1, demonstrated a lateral mobility for membrane wheat germ agglutinin (WGA) and succinylated concanavalin A (sCon A) receptors of (2.1 +/- 1.6) X 10(-9) cm2/s and (2.7 +/- 2.3) X 10(-9) cm2/s, respectively. These mobilities were approximately equivalent to phospholipid mobility (2.8 +/- 1.9 X 10(-9) cm2/s). The fast mobility phenotype is observed when the cells are unattached and spherical. Upon attachment, the mobility decreases to (0.19 +/- 0.19) X 10(-9) cm2/s. In addition, the ability of Con A to initiate global modulation was completely lost in spread as well as spherical cells in the E7G1 fast mobility clone. A comparison of F-actin patterns between untransformed Balb/c fibroblasts and the E7G1-transformed line suggests a correlation between well-developed stress fiber assemblies and the ability to induce global modulation. The fast mobility clone was stable for at least 23 passages.
Swaisgood
M
M
Department of Biochemistry, Michigan State University, East Lansing 48824.
Schindler
M
M
D000199:Actins / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002461:Cell Line, Transformed; D002465:Cell Movement; D002999:Clone Cells / Q000502:physiology; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D005452:Fluoresceins; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D010743:Phospholipids / Q000502:physiology; D010777:Photochemistry; D011952:Receptors, Concanavalin A / Q000502:physiology; D011975:Receptors, Mitogen / Q000032:analysis / Q000502:physiology; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D000199:Actins; D005353:Fibronectins; D005452:Fluoresceins; D010743:Phospholipids; D011952:Receptors, Concanavalin A; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; C027503:wheat germ agglutinin receptor