Identification of a U5-specific sequence required for efficient polyadenylation within the human immunodeficiency virus long terminal repeat.
Retrovirus mRNAs are normally polyadenylated within the proviral 3' long terminal repeat (LTR). The site of retrovirus transcript polyadenylation is flanked 3' by an LTR-specific sequence termed the U5 region, but the role of U5 in the determination of polyadenylation efficiency has not been addressed. We have used site-directed mutagenesis of a human immunodeficiency virus LTR to map U5 sequences which are required for efficient polyadenylation within the LTR. These LTR U5 region sequences display homology to a motif termed the G-T cluster, which is known to facilitate the efficient polyadenylation of mRNAs encoded by several cellular and viral genes. These results suggest that the LTR U5 region functions in vivo to permit efficient polyadenylation within the proviral 3' LTR.
Böhnlein
S
S
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
Hauber
J
J
Cullen
B R
BR
D001483:Base Sequence; D002460:Cell Line; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D011061:Poly A / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D012367:RNA, Viral; D011061:Poly A