Association of endogenous synenkephalin containing peptides with intracellular membranes of bovine adrenal medulla.
The association of endogenous synenkephalin and met-enkephalin containing peptides with the membrane of bovine chromaffin granules and physicochemical characteristics of this association were studied. The associated materials were only released at a non physiological pH range and this effect was enhanced with growing salt concentrations (0.5, 1.0 and 2.0 M KSCN). A higher peptide dissociation occurred with membrane solubilizing agents (SDS greater than Triton X-100 greater than digitonin). In microsomes the materials dissociated with 2 M KSCN (pH 7.4) corresponded to peptides larger than 12.0 kDa, while in granules corresponded to molecules smaller than 8.5 kDa, displaying synenkephalin and met-enkephalin immunoreactivities. These data suggest that some sequence of the C-terminal portion of synenkephalin may be responsible for the association of proenkephalin derived peptides with microsome and granule membranes.
Ase
A
A
Instituto de Investigaciones Médicas Sección Sustancias Vasoactivas, Buenos Aires, Argentina.
Vindrola
O
O
Aloyz
R
R
Inés
M
M
Vida
R
R
Finkielman
S
S
Nahmod
V
V
D000313:Adrenal Medulla / Q000648:ultrastructure*; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002837:Chromaffin Granules / Q000648:ultrastructure; D002850:Chromatography, Gel; D004072:Digitonin / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000378:metabolism*; D004745:Enkephalins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D008861:Microsomes / Q000648:ultrastructure; D008970:Molecular Weight; D017830:Octoxynol; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000494:pharmacology; D011498:Protein Precursors / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology
D004745:Enkephalins; D011498:Protein Precursors; D013861:Thiocyanates; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D004744:Enkephalin, Methionine; C036693:synenkephalin; D017830:Octoxynol; D004072:Digitonin; C009941:potassium thiocyanate