Identification of a leukocyte alloantigen with a high-frequency expression in leukemia patients.
In this report we describe the production and characterization of a monoclonal antibody to the human promyelocytic leukemia cell line HL-60. The antibody, NC-2, is of the IgG1 subclass and precipitates a 50-Kd protein from 125I-labeled HL-60 cells. The antigen is insensitive to treatment with trypsin, papain, or neuraminidase. NC-2 did not react with a number of established human cell lines, including Daudi, Molt-4, K562, U937, KG-1, CEM, Raji, and Gash-P. Neutrophils and monocytelike cells derived from HL-60 cells that were induced to differentiate continued to express the antigen. NC-2 reacted with all peripheral-blood cells except erythrocytes from eight (5%) of 150 normal individuals tested. Bone marrow samples from patients with myelogenous leukemias were more frequently reactive with NC-2 than were those from normal individuals (12/33 v 1/10). Family studies indicated that the antigen was inherited in an autosomal-dominant manner. These findings suggest that the expression of the above alloantigen is associated with an increased incidence of leukemia.
O'Connor
R
R
Department of Biology, St. Patrick's College, Maynooth, Ireland.
Bradley
J G
JG
O'Meara
A
A
Cotter
T G
TG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D001666:Binding Sites, Antibody; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D006801:Humans; D007519:Isoantigens / Q000032:analysis* / Q000276:immunology; D007938:Leukemia / Q000097:blood / Q000235:genetics / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase; D010206:Papain; D014357:Trypsin
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D007519:Isoantigens; D009439:Neuraminidase; D014357:Trypsin; D010206:Papain