The role of sulfhydryl groups in sulfobromophthalein transport in rat liver plasma membrane vesicles.
Sulfobromophthalein (BSP) electrogenic transport activity in a plasma membrane vesicle preparation from rat liver is shown to depend on free sulfhydryl groups. These are organized in two classes, one of which does not react with the sulfhydryl group reagent 5,5'-dithiobis(2-nitrobenzoate). The two classes appear to be involved in BSP transport independently. However, reactivity of one class can be shown to be affected by alkylation of the other. Hence, it is concluded that both classes are located on the same carrier system, which previous research has established to be the integral sinusoidal membrane protein bilitranslocase.
Passamonti
S
S
Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole, Università degli Studi di Trieste, Italy.
Sottocasa
G L
GL
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology; D019327:Copper Sulfate; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008623:Mercaptoethanol / Q000494:pharmacology; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013448:Sulfobromophthalein / Q000378:metabolism*
D006902:Hydroxymercuribenzoates; D013438:Sulfhydryl Compounds; D013448:Sulfobromophthalein; C030601:4-hydroxymercuribenzoate; D008623:Mercaptoethanol; D003300:Copper; D004228:Dithionitrobenzoic Acid; D019327:Copper Sulfate; D005033:Ethylmaleimide