Quantitation of strand breaks in human DNA using 32P-Alu hybridization: application to exponential and plateau-phase cells.
A sensitive quantitation of DNA (0.2 to 10 ng) can be achieved using a 32P-labeled Alu probe to hybridize human DNA spotted onto nylon membrane. This allows the determination of radiation-induced single-strand breaks without the use of [3H]thymidine prelabeling of cells in culture. The sensitivity of this technique in HeLa cells is comparable to results obtained using the alkaline unwinding technique. The method is applicable to cells in both exponential and plateau phases of growth.
Prasad
S C
SC
Department of Radiation Medicine, Vincent T. Lombardi Comprehensive Cancer Research Center, Georgetown University Medical Center, Washington, D.C. 20007.
Boyle
J
J
Dritschilo
A
A
D002588:Cesium Radioisotopes; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D015342:DNA Probes; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D005347:Fibroblasts; D006367:HeLa Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D012091:Repetitive Sequences, Nucleic Acid
D002588:Cesium Radioisotopes; D015342:DNA Probes; D004277:DNA, Single-Stranded; D010761:Phosphorus Radioisotopes; D004247:DNA