title: Structure and properties of a human non-pancreatic phospholipase A2.
abstract: We have purified a human non-pancreatic phospholipase A2 that is present in platelets and is enriched in rheumatoid synovial fluid. The enzyme is calcium-dependent, has a pH optimum of 8-10, and shows a striking preference for substrate presented in the form of Escherichia coli membranes. In the E. coli phospholipase A2 assay the phospholipase exhibits an apparent specific activity of 300 mumol/mg/min. Using oligonucleotide probes based on amino-terminal sequence data, we cloned the corresponding human gene from a genomic DNA library and expressed the gene in animal cells. The protein was secreted from the cells in an active form. The deduced amino acid sequence of the human protein consists of 124 amino acids, contains structural features common to all known phospholipase A2s, and has a half-cystine pattern that is characteristic of the snake venom group II enzymes.
authors.affiliation: Biogen Inc., Cambridge, Massachusetts 02142.
authors.forename: R M
authors.initials: RM
authors.lastname: Kramer
authors.forename: C
authors.initials: C
authors.lastname: Hession
authors.forename: B
authors.initials: B
authors.lastname: Johansen
authors.forename: G
authors.initials: G
authors.lastname: Hayes
authors.forename: P
authors.initials: P
authors.lastname: McGray
authors.forename: E P
authors.initials: EP
authors.lastname: Chow
authors.forename: R
authors.initials: R
authors.lastname: Tizard
authors.forename: R B
authors.initials: RB
authors.lastname: Pepinsky
mesh_terms: D000595:Amino Acid Sequence; D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D001483:Base Sequence; D001792:Blood Platelets / Q000201:enzymology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005796:Genes; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D010179:Pancreas / Q000201:enzymology; D010740:Phospholipases / Q000235:genetics*; D010741:Phospholipases A / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D054467:Phospholipases A2; D015183:Restriction Mapping; D013582:Synovial Fluid / Q000201:enzymology*
chemical_list: D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2