Production of toxic shock syndrome toxin 1 in a mouse model of Staphylococcus aureus abscess formation.
A mouse model of abscess formation is described in which toxic shock syndrome toxin 1 (TSST-1) is produced by Staphylococcus aureus in vivo. Mice injected intravenously with S. aureus developed renal abscesses within 4-7 days. Kidneys excised from infected mice were cultured quantitatively, and extracts from the kidneys were assayed for TSST-1 with use of a competitive enzyme-linked immunosorbent assay. Animals with less than 10(7) S. aureus/g of kidney had less than 6 ng of TSST-1/mL of extract. Toxin levels ranged from less than 6 ng/mL to 271 ng/mL in kidney extracts from mice with greater than 10(7) S. aureus/g of kidney. Urine from infected mice also contained measurable levels of TSST-1 (range, less than 6-728 ng/mL). Mice developed serum antibodies to TSST-1 by 2 weeks after challenge. Serum samples collected 5-7 days after bacterial challenge did not show biochemical changes typical of a toxic shock-like illness in these animals.
Lee
J C
JC
Channing Laboratory, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Perez
N E
NE
Hopkins
C A
CA
D000038:Abscess / Q000382:microbiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001427:Bacterial Toxins; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007668:Kidney / Q000032:analysis; D007674:Kidney Diseases / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000276:immunology / Q000378:metabolism*; D018089:Superantigens
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal