Antibodies raised against denatured DNA bind to double-stranded DNA.
Antibodies raised against denatured DNA complexed with methylated bovine serum albumin have been reported to react with ssDNA but not with dsDNA. Using a highly sensitive avidin-biotin microELISA, we report that such antibodies also bind to dsDNA. Antibodies which reacted with ssDNA and dsDNA were found to be of IgG type. The antibodies did not react with tRNA and rRNA. The binding of antibodies to dsDNA was partially inhibited by individual deoxyribonucleotides. ssDNA as well as dsDNA inhibited the binding of antibodies to dsDNA. The binding of these antibodies to supercoiled and relaxed forms of pBR322 DNA was demonstrated by gel retardation assay. The cross-reaction with ssDNA was observed even after affinity purification on native DNA-cellulose. The antibodies were also shown to bind to poly(dA-dT).poly(dA-dT).
Vaishnav
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YN
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Antony
A
A
D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D004247:DNA / Q000276:immunology* / Q000648:ultrastructure; D004278:DNA, Superhelical / Q000276:immunology; D003854:Deoxyribonucleotides / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D009691:Nucleic Acid Denaturation; D010957:Plasmids; D011817:Rabbits
D004278:DNA, Superhelical; D003854:Deoxyribonucleotides; D004247:DNA