Free 3-methoxy-4-hydroxyphenylglycol determined in plasma by liquid chromatography with coulometric detection.
We describe a simple "high-performance" liquid-chromatographic method for determining free 3-methoxy-4-hydroxyphenylglycol (MHPG) in plasma, with coulometric detection and with 4-methoxy-3-hydroxyphenylglycol (iso-MHPG) as the internal standard. MHPG and iso-MHPG are extracted from plasma into ethyl acetate and the extract is washed with a sodium bicarbonate solution, evaporated, reconstituted, and injected into a 25 x 0.4 cm column packed with 3-micron particles of C18 material. We used a mobile phase of acetate buffer (100 mmol/L, pH 5.0) and methanol (92:8 by vol) and an oxidation voltage of 0.5 V. The detection limit of the assay is 0.1 micrograms/L. The interassay CV for a sample with a mean MHPG concentration of 3.84 micrograms/L was 5.2%. The average absolute recovery for the method was 37%.
Hariharan
M
M
Department of Psychiatry, University of Michigan Medical School, Ann Arbor 48109-0656.
VanNoord
T
T
Cameron
O G
OG
Curtis
G C
GC
Ostrow
D G
DG
D000328:Adult; D001008:Anxiety Disorders / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003866:Depressive Disorder / Q000097:blood; D004563:Electrochemistry; D005260:Female; D006018:Glycols / Q000097:blood*; D006801:Humans; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000097:blood*
D006018:Glycols; D008734:Methoxyhydroxyphenylglycol