title: The role of long-chain acyl-CoA in the damage of oxidative phosphorylation in heart mitochondria.
abstract: The aim of this investigation was to study the effect of intramitochondrial acyl-CoA on the respiration of rabbit heart mitochondria over the whole range of stationary respiratory rates between States 4 and 3. The creatine phosphokinase system was used for stabilization of extramitochondrial adenine nucleotide concentration. It was shown that acyl-CoA depressed respiration more effectively in the intermediate range of respiration between States 4 and 3. The effect of acyl-CoA was negligible near State 4 and in State 3. These data are in line with our previous results concerning the dependence of the adenine nucleotide translocator control coefficient on the rate of mitochondrial respiration. Thus, our data suggest that long-chain acyl-CoA may regulate oxidative phosphorylation in heart mitochondria in vivo.
authors.affiliation: Central Research Laboratory, Kaunas Medical Institute, USSR.
authors.forename: V
authors.initials: V
authors.lastname: Borutaite
authors.forename: V
authors.initials: V
authors.lastname: Mildaziene
authors.forename: L
authors.initials: L
authors.lastname: Ivanoviene
authors.forename: B
authors.initials: B
authors.lastname: Kholodenko
authors.forename: A
authors.initials: A
authors.lastname: Toleikis
authors.forename: A
authors.initials: A
authors.lastname: Praskevicius
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000201:enzymology* / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000187:drug effects; D010172:Palmitoylcarnitine / Q000494:pharmacology; D011817:Rabbits; D012402:Rotenone / Q000494:pharmacology
chemical_list: D000214:Acyl Coenzyme A; D012402:Rotenone; D010172:Palmitoylcarnitine; D000226:Mitochondrial ADP, ATP Translocases