Effects of lys-beta-urogastrone in vivo.
Lys-beta-urogastrone, an analogue of human beta-urogastrone with an additional N-terminal lysine, was shown to have similar effects in mice and sheep to mouse epidermal growth factor (mEGF). Lys-beta-urogastrone in doses of 0.18-3.24 micrograms g-1 body weight caused both precocious separation of eyelids and eruption of incisors in neonatal mice. In 17 sheep, intravenous infusion of the urogastrone analogue over c. 24 h led, towards the end of infusion, to erythema of the muzzle, caused reductions in voluntary food intake (with doses greater than or equal to 50 micrograms kg-1) and generally easier manual harvesting of the fleece (with infusions greater than or equal to 81 micrograms kg-1), with spontaneous shedding of the fleece (c. 14 days after infusions of greater than or equal to 116 micrograms kg-1). In five sheep infusions of 25, 38, 50, 83 and 118 micrograms kg-1 fleece-free body weight, plasma concentrations of lys-beta-urogastrone were near maximal 20 h after the infusions started and were, respectively, 1.1, 1.7, 5.5, 18 and 79 micrograms l-1 plasma. Plasma concentrations of gastrin, somatostatin and pancreatic polypeptide were determined in these five sheep. Plasma gastrin rose sixfold by the end of infusions of 25 micrograms kg-1 of the urogastrone analogue, and tenfold with the higher doses of infusion. Although plasma somatostatin concentrations were variable, a consistent trend was observed; lower levels were apparent during the lys-beta-urogastrone infusions. There was no discernible trend in pancreatic polypeptide concentrations.
Campbell
A J
AJ
ICI Australia Operations Pty Ltd, Research Group, Ascot Vale.
Adams
S S
SS
Davey
M W
MW
Titchen
D A
DA
D000818:Animals; D000833:Animals, Suckling / Q000254:growth & development; D004435:Eating / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D004890:Erythema / Q000139:chemically induced; D005143:Eyelids / Q000187:drug effects / Q000254:growth & development; D005260:Female; D005755:Gastrins / Q000097:blood; D007180:Incisor / Q000187:drug effects / Q000254:growth & development; D008297:Male; D051379:Mice; D010191:Pancreatic Polypeptide / Q000097:blood; D012756:Sheep / Q000502:physiology*; D013004:Somatostatin / Q000097:blood; D014935:Wool / Q000187:drug effects* / Q000254:growth & development
D005755:Gastrins; C062721:urogastrone, Lys-beta-; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D004815:Epidermal Growth Factor