title: Trypanosoma brucei: frequent loss of a telomeric variant surface glycoprotein gene.
abstract: We have observed the loss of an inactive telomeric variant surface glycoprotein (VSG) gene that is located on a minichromosome in Trypanosoma brucei. If this is due to gene conversion, it is the third "silent" gene conversion (i.e., one that does not produce an antigenic switch) detected in 19 antigenic switches of the IsTaR 1 serodeme. This is surprisingly frequent since the immune response cannot select against the inactive gene. We estimate that 10(-1) to 10(-3) telomeric VSG gene conversions occur per generation, which is at least 100 times more frequent than antigenic switching. Since all three "silent" gene conversions involved an IsTat 5 VSG gene, the frequency may vary among telomeric VSG genes. However, the high gene conversion frequency for the 5 VSG gene does not ensure a higher antigenic switch frequency than other telomeric VSG genes for which we have probes. These results suggest that gene conversion rapidly alters the repertoire of telomeric VSG genes, possibly including those on minichromosomes, producing a continual variation in the VSG genes that are more likely to be expressed.
authors.affiliation: Seattle Biomedical Research Institute, Washington 98109-1651.
authors.forename: R F
authors.initials: RF
authors.lastname: Aline
authors.forename: P J
authors.initials: PJ
authors.lastname: Myler
authors.forename: K D
authors.initials: KD
authors.lastname: Stuart
mesh_terms: D000818:Animals; D000940:Antigenic Variation; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology; D002875:Chromosomes; D002999:Clone Cells; D005785:Gene Conversion; D005796:Genes; D051379:Mice; D015183:Restriction Mapping; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000276:immunology; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D014643:Variant Surface Glycoproteins, Trypanosoma