title: Eosinophilic spleen colonies are produced in rat-marrow-transplanted but not in murine-marrow-transplanted mice.
abstract: Differential counts of about 5000 splenic clusters and colonies developing in whole-body-irradiated mice and rats were made, using semi-serial histological sections prepared 9 to 12 d after transplantation with bone marrow haemopoietic cells. The investigated mouse and rat spleens were from syngeneically, allogeneically, or xenogeneically transplanted recipients. Splenic eosinophil clusters were always found when rat eosinophil-producing progenitors were present in the inoculum, whereas murine inocula failed to produce splenic eosinophilic clusters even in the syngeneic mouse. The limiting factor in the production of splenic eosinophilic clusters was the appropriate donor progenitor/committed stem cell itself. Changes in the percentages of eosinophil clusters with the number of injected cells and with increased doses of irradiation, as well as formation of rat eosinophil colonies in mice, as against mainly clusters in rats, themselves show that regulatory mechanisms of the recipients also play a role. These regulatory mechanisms cannot be attributed to the splenic microenvironment.
authors.affiliation: Department of Pathology, City Hospital, Hatvan, Hungary.
authors.forename: L G
authors.initials: LG
authors.lastname: Szabó
authors.forename: E
authors.initials: E
authors.lastname: Kelemen
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D004804:Eosinophils / Q000166:cytology*; D006085:Graft Survival; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology / Q000502:physiology*; D008808:Mice, Inbred CBA / Q000276:immunology / Q000502:physiology*; D011828:Radiation Chimera; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology / Q000502:physiology*; D013045:Species Specificity; D013154:Spleen / Q000166:cytology*; D014183:Transplantation, Heterologous; D014184:Transplantation, Homologous