title: Studies of a glomerular permeability factor in patients with minimal-change nephrotic syndrome.
abstract: Peripheral blood mononuclear cells (PBMC) from patients with minimal-change nephrotic syndrome (MCNS) were tested for their ability to produce a factor which increases the urinary protein excretion levels of rats. It was shown that enhanced proteinuria can be produced in 8-hour urine specimens from rats by the injection of concentrated supernatants of cultured concanavalin-A-stimulated PBMC of patients with MCNS, but not from other nephrotics or normal subjects. The increase in urinary protein excretion was associated with a significant alteration of glomerular epithelial cells similar to that seen in MCNS. These results suggest that in MCNS, PBMC release a factor, which we termed a glomerular permeability factor (GPF), causing changes in glomerular permeability with resulting proteinuria.
authors.affiliation: Second Department of Internal Medicine, National Defense Medical College, Tokorozawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yoshizawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kusumi
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: S
authors.initials: S
authors.lastname: Oshima
authors.forename: A
authors.initials: A
authors.lastname: Takeuchi
authors.forename: O
authors.initials: O
authors.lastname: Kawamura
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Kondo
authors.forename: H
authors.initials: H
authors.lastname: Niwa
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006801:Humans; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000648:ultrastructure; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009402:Nephrosis, Lipoid / Q000097:blood*; D010539:Permeability; D011507:Proteinuria / Q000209:etiology / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D014556:Urine
chemical_list: D003208:Concanavalin A