Carrier-bound synthetic peptides. Use as antigen in HIV-1 ELISA tests and in antiserum production.
Chemically synthesize carrier-bound peptides have been used as antigens in diagnostic test systems (ELISA) and for raising antipeptide-specific antisera. The method does not require prior cleavage of the peptides from the support used for the solid-phase synthesis. Using the same resin for both the synthesis and the subsequent applications it was possible to avoid expensive and time-consuming purification procedures and artificial recoupling to solid supports. A quick and specific ELISA-based diagnostic test system for HIV-specific antipeptide antibodies in human sera was established. In addition the carrier-bound peptides were shown to be potent antigens for raising antibodies in animals.
Modrow
S
S
Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, F.R.G.
Höflacher
B
B
Mertz
R
R
Wolf
H
H
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000138:chemical synthesis*; D055598:Chemical Phenomena; D002621:Chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006679:HIV Seropositivity / Q000175:diagnosis; D006801:Humans; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011137:Polystyrenes; D011817:Rabbits
D000941:Antigens; D010455:Peptides; D011137:Polystyrenes