Screening for microalbuminuria by use of a rapid, low-cost colorimetric assay.
We evaluated the pyrogallol red-molybdate(IV) method for quantification of urinary protein as a screening procedure for microalbuminuria by determining the assay's sensitivity and specificity at different concentrations of urinary albumin as measured by a comparison laser-nephelometric immunoassay. The pyrogallol red-molybdate(IV) method has sensitivity and specificity similar to that for other semiquantitative assays, but it is less expensive and sample throughput can be high if microtiter plate techniques are used.
Phillipou
G
G
Endocrine and Diabetes Laboratory, Queen Elizabeth Hospital, Woodville, South Australia.
James
S K
SK
Seaborn
C J
CJ
Phillips
P J
PJ
D000419:Albuminuria / Q000652:urine*; D003124:Colorimetry; D004396:Coloring Agents; D003920:Diabetes Mellitus / Q000652:urine*; D006801:Humans; D007118:Immunoassay; D008982:Molybdenum; D009391:Nephelometry and Turbidimetry; D011748:Pyrogallol / Q000031:analogs & derivatives; D012016:Reference Values
D004396:Coloring Agents; D011748:Pyrogallol; C044659:molybdate; C058731:pyrogallol sulfonphthalein; D008982:Molybdenum