title: Differential assemblage of the basal membrane-cytoskeleton complex in bovine epithelial lens cells.
abstract: We have investigated the membrane-cytoskeleton complex involved in interactions between the epithelial cells and the capsule of the bovine eye lens. The organization of the molecular complex was determined from cell extraction, immunoprecipitation and immunoblotting experiments and from ultrastructural studies by scanning electron microscopy. We show that marked differences exist in the organization of this basal complex between the central epithelium (mitotic quiescent) and the peripheral epithelium which initiates lenticular differentiation into fibres. Our results support the view that: (a) the organization of several major membrane components in the peripheral epithelium differs from that of the central epithelium; (b) microfilaments and vimentin filaments are independent of each other in the peripheral epithelium, whereas microfilaments are involved in an inter-relationship with vimentin filaments in the central epithelium; (c) two surface proteins of 24 and 27 kD and two surface glycoproteins of 46 and 220 kD appear to be bound to vimentin filaments in the peripheral region, whereas the intermediate filaments appear to be solely in close association with the 46 and 220 kD glycoproteins in the central zone.
authors.affiliation: INSERM U.118, CNRS UA 630, Paris, France.
authors.forename: P R
authors.initials: PR
authors.lastname: Blanquet
authors.forename: Y
authors.initials: Y
authors.lastname: Courtois
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000199:Actins / Q000032:analysis; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002462:Cell Membrane / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D004848:Epithelium / Q000648:ultrastructure; D006023:Glycoproteins / Q000032:analysis; D015151:Immunoblotting; D007903:Lens Capsule, Crystalline / Q000648:ultrastructure; D007908:Lens, Crystalline / Q000032:analysis / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D014746:Vimentin / Q000032:analysis
chemical_list: D000199:Actins; D006023:Glycoproteins; D008565:Membrane Proteins; D014746:Vimentin