title: A rapid and efficient purification of poly(A)-mRNA by oligo(dT)30-Latex.
abstract: Latex particles were covalently linked to the 5'-proximal region of oligo(dT)30. The resultant oligo(dT)30-Latex was tested for its hybridizability to poly(A) containing mRNA. Several advantages were noted as compared to the conventional oligo(dT)30-cellulose column chromatography; (1) a highly efficient (approximately 95%) hybridization occurs in a short reaction period (10min), (2) more than 95% of poly(A) mRNA can be recovered from oligo(dT)30-Latex by a simple heating followed by brief centrifugation, (3) multiple samples can be handled simultaneously and moreover, (4) the poly(A)-mRNA on the oligo(dT)30-Latex can be directly transcribed by AMV reverse transcriptase to form the cDNA. These properties of oligo(dT)30-Latex promise an excellent reagent for nucleic acid technology.
authors.affiliation: Japan Synthetic Rubber Co., Ltd., Tokyo Research Laboratory, Kawasaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kuribayashi
authors.forename: M
authors.initials: M
authors.lastname: Hikata
authors.forename: O
authors.initials: O
authors.lastname: Hiraoka
authors.forename: C
authors.initials: C
authors.lastname: Miyamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Furuichi
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D004279:DNA, Viral / Q000235:genetics; D007202:Indicators and Reagents; D007840:Latex; D009838:Oligodeoxyribonucleotides; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D004279:DNA, Viral; D007202:Indicators and Reagents; D007840:Latex; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C027903:oligo (dT); D011061:Poly A