Effect of low-energy laser irradiation on colony formation capability in different human tumor cells in vitro.
Fibroblasts and lymphocytes are the most widely used cells for studying the so-called biostimulative effect of low-power laser in vitro. In contrast, stimulation of cancer cells by laser light has not been investigated extensively. The present study attempted to evaluate whether or not human tumor cells could exhibit an increase in colony-forming capability following low-watt laser irradiation. LoVo and HT29 (colon carcinoma), MCF7 (breast carcinoma), M14 and JR1 (malignant melanoma) cell lines were irradiated at different doses of light delivered from an argon or an argon-dye laser. Radiant exposures between 4.2 and 150 kJ/m2 at irradiances ranging from 35 to 500 W/m2 were delivered. Results were mixed. Of the 41 experiments performed, five showed a significant statistical increase in the number of colonies (P less than 0.05), whereas three showed a decrease (P less than 0.05). Nevertheless, the trend of most data was toward an increase in colony formation, and Wilcoxon's signed-ranks test suggested that light increases tumor cell culture growth (P less than 0.03).
Marchesini
R
R
Department of Health Physics, Istituto Nazionale Tumori di Milano, Italy.
Dasdia
T
T
Melloni
E
E
Rocca
E
E
D001943:Breast Neoplasms / Q000473:pathology; D002455:Cell Division / Q000528:radiation effects; D003110:Colonic Neoplasms / Q000473:pathology; D003114:Colony-Forming Units Assay; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers; D008545:Melanoma / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014410:Tumor Stem Cell Assay