Aggregated human colostral sIgA stimulates delayed, non-complement-dependent, NBT reduction by human neutrophils.
Antibodies often alert polymorphonuclear neutrophils (PMNs) to the presence of pathogens. In a study to learn if secretory immunoglobulins can carry out this function, we observed that as little as 4 micrograms/ml of secreted human immunoglobulin A from colostrum (sIgA), in the absence of antigen, stimulated human PMNs to reduce nitroblue tetrazolium (NBT). NBT reduction was inhibited 71% by superoxide dismutase. Active complement pathways were not required since comparable activity was obtained in the presence of heat-inactivated serum. Aggregated forms of sIgA were much more stimulatory than nonaggregated dimeric sIgA. Such interaction between PMNs and sIgA could act in situ to enhance protection against infections of exposed body sites or could initiate inflammatory tissue damage.
Bullock
W W
WW
Department of Oral Microbiology/Immunology, Oregon Health Sciences University, Portland 97201.
Wang
Y Z
YZ
Gabler
W L
WL
Creamer
H R
HR
D003167:Complement Activation; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D011247:Pregnancy
D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D009580:Nitroblue Tetrazolium; D009240:N-Formylmethionine Leucyl-Phenylalanine