Role of fibronectin and fibrinogen in healing of corneal epithelial scrape wounds.
A provisional fibronectin (Fn)-fibrinogen (Fg) matrix forms de novo on the bare basement membrane (BM) surface of superficial epithelial scrape wounds in rabbit and guinea pig (GP) corneas. We determined whether such a substrate is essential for epithelial cell adhesion and migration, using three different approaches. (1) Polyclonal and monoclonal IgG reactive against GP Fn were topically administered to inhibit Fn formation in a GP epithelial scrape wound model. Immunofluorescence studies showed that deposition of both Fn and Fg was inhibited in antibody-treated corneas. During the first 38 hr after wounding, the healing rates were 0.46 +/- 0.06 mm2/hr in control eyes, 0.43 +/- 0.05 mm2/hr in those treated with rabbit polyclonal IgG anti-GP Fn and 0.45 +/- 0.09 mm2/hr in those treated with murine monoclonal IgG anti-GP Fn (P greater than 0.4). (2) In a rabbit epithelial scrape wound model, ancrod was administered intravenously to induce systemic Fg depletion. Fg deposition was completely inhibited on the wound surface, but Fn deposition was not suppressed. The healing rate was 1.24 +/- 0.41 mm2/hr in ancrod-treated corneal wounds and 1.19 +/- 0.29 mm2/hr in control eyes during the first 48 hr after wounding (P greater than 0.5). These data from the antibody and ancrod inhibition indicate that Fg binds to Fn and that Fn binds to components other than Fg.(ABSTRACT TRUNCATED AT 250 WORDS)
Phan
T M
TM
Department of Pathology, Massachusetts General Hospital, Boston 02114.
Foster
C S
CS
Wasson
P J
PJ
Fujikawa
L S
LS
Zagachin
L M
LM
Colvin
R B
RB
D000721:Ancrod / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003315:Cornea / Q000473:pathology / Q000503:physiopathology; D065306:Corneal Injuries; D004848:Epithelium / Q000503:physiopathology; D005340:Fibrinogen / Q000037:antagonists & inhibitors / Q000502:physiology*; D005353:Fibronectins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000276:immunology; D007150:Immunohistochemistry; D009924:Organ Culture Techniques; D014945:Wound Healing; D014950:Wounds, Penetrating / Q000473:pathology / Q000503:physiopathology*
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007074:Immunoglobulin G; D005340:Fibrinogen; D000721:Ancrod