Stainless steel mesh supports high density cell growth and production of recombinant müllerian inhibiting substances.
Stainless steel mesh supported the high density growth of anchorage dependent CHO fibroblasts without the use of a special culture system. CHO cells, designated B-9, containing an amplified genomic construct of the human gene for Müllerian Inhibiting Substance (MIS), grew to a high confluent density on stainless steel meshwork while producing substantial amounts of human recombinant MIS over a long period of time. The mesh could be easily coated with various extracellular matrix proteins, such as Laminin, Fibronectin, Collagen or Matrigel, which permitted the testing of the effects of surface modifications on cell yield and recombinant protein production. Since the amount of medium per surface area required for optimal cell growth is lower than for some large volume cell culture methods, media costs can be reduced using mesh. In addition, no special cell culture equipment or complex manipulations are required. Thus, the use of meshwork for anchorage-dependent cells can increase the efficiency of growth and decrease the cost of recombinant protein production.
Epstein
J
J
Pediatric Surgical Research Laboratory, Massachusetts General Hospital, Boston 02114.
Desjardins
E J
EJ
Hudson
P L
PL
Donahoe
P K
PK
D000818:Animals; D054304:Anti-Mullerian Hormone; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line / Q000166:cytology*; D003094:Collagen / Q000494:pharmacology; D005109:Extracellular Matrix; D006023:Glycoproteins; D006131:Growth Inhibitors; D008562:Membrane Glycoproteins / Q000494:pharmacology; D008967:Molecular Biology / Q000295:instrumentation; D011994:Recombinant Proteins / Q000096:biosynthesis; D013193:Stainless Steel; D013499:Surface Properties; D013735:Testicular Hormones / Q000096:biosynthesis*
D006023:Glycoproteins; D006131:Growth Inhibitors; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D013735:Testicular Hormones; D013193:Stainless Steel; D054304:Anti-Mullerian Hormone; D003094:Collagen