An agarose gel electrophoretic method for typing phosphoglucomutase-1, esterase D, or glyoxalase I.
A conventional agarose gel electrophoretic method was described for typing phosphoglucomutase-1, esterase D, or glyoxalase I as single systems. Bloodstain extracts were absorbed into 1-mm-thick agarose gels via an application mask. The electrode wick distance was 12 cm and electrophoresis was carried out at 400 V at 6 degrees C. The electrophoretic run times were 30 min for glyoxalase and 1 h for esterase D or phosphoglucomutase. This method is reliable and produces highly resolved band patterns. Additionally, the shorter separation times as a result of the increased voltage gradient permitted typing of more samples in a given time period compared with presently used methods. This technique requires little technical expertise and can be incorporated into the laboratory at a minimal cost.
Budowle
B
B
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001801:Blood Stains; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000235:genetics*; D008190:Lyases / Q000235:genetics*; D010641:Phenotype; D010733:Phosphoglucomutase / Q000235:genetics*
D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human; D008190:Lyases; D007791:Lactoylglutathione Lyase; D010733:Phosphoglucomutase