Amino acid sequence of locust neuroparsins.
Neuroparsins A and B were isolated from the nervous part of the corpus cardiaca of Locusta migratoria via a two-step purification procedure. Both consist of two polypeptide chains linked by disulfide bridges. The N-terminal sequence of both native neuroparsins was determined: the N-terminal end of neuroparsin B was unique while that of neuroparsin A showed three different sequences. These sequences were that of neuroparsin B and two others having five and two extra N-terminal residues. Neuroparsin B was found as a homodimer and the complete sequence of the monomer, determined from peptide fragments generated by treatment with cyanogen bromide and endoprotease Glu-C, comprises 78 residues.
Girardie
J
J
Laboratoire de Neuroendocrinologie, UA CNRS 1138, Université de Bordeaux I, Talence, France.
Girardie
A
A
Huet
J C
JC
Pernollet
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D005260:Female; D006110:Grasshoppers / Q000032:analysis*; D007301:Insect Hormones / Q000302:isolation & purification; D008297:Male; D008969:Molecular Sequence Data; D009490:Neurosecretory Systems / Q000032:analysis*; D011487:Protein Conformation
D007301:Insect Hormones; C058919:neuroparsin A; C058920:neuroparsin B; D003488:Cyanogen Bromide