title: Derivatization of ketosteroids for fast atom bombardment mass spectrometry.
abstract: Girard's reagents were used to derivatize ketosteroids and conjugates for analysis by positive ion fast atom bombardment mass spectrometry. Spectra contain an abundant ion corresponding to the cation (C+) of the newly formed ionic derivative (C+A-) and relatively little fragmentation. With derivatization, detection of ketosteroids at a concentration of 1 microgram microliter-1 in glycerol was straightforward. Such derivatization schemes may prove useful in the analysis of ketosteroids in complex biological mixtures.
authors.forename: G C
authors.initials: GC
authors.lastname: DiDonato
authors.forename: K L
authors.initials: KL
authors.lastname: Busch
mesh_terms: D007202:Indicators and Reagents; D007664:Ketosteroids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D008832:Microchemistry
chemical_list: D007202:Indicators and Reagents; D007664:Ketosteroids