Spermidine/spermine-N1-acetyltransferase activity in isoprenaline-stimulated rat salivary glands.
In parotid, sublingual and submaxillary glands stimulated by continuous intravenous infusion of isoprenaline at various doses for 3 h, the concentrations of the polyamines putrescine, spermidine, spermine and N1-acetylspermidine as well as the activities of ornithine decarboxylase and spermidine/spermine-N1-acetyltransferase were determined. Ornithine decarboxylase is the enzyme that catalyses the formation of putrescine from ornithine. The most striking effect was observed in parotid glands. Here, the beta-adrenergic agonist increased the activity of spermidine/spermine-N1-acetyltransferase markedly, but only slightly (if at all) that of ornithine decarboxylase. It also increased the concentrations of N1-acetylspermidine and putrescine markedly. The result obtained in this gland indicates interconversion of higher polyamines to putrescine.
Ekström
J
J
Department of Physiology and Biophysics, Medical Faculty, Lund University, Sweden.
Månsson
B
B
Nilsson
B O
BO
Rosengren
E
E
Tobin
G
G
D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D007262:Infusions, Intravenous; D007545:Isoproterenol / Q000008:administration & dosage*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000201:enzymology*; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D007545:Isoproterenol; D009955:Ornithine Decarboxylase; D011700:Putrescine; D013095:Spermidine; D013096:Spermine; D000099228:Diamine N-Acetyltransferase