Human T cell recognition of cloned HLA class I gene products expressed on DNA transfectants of mouse mastocytoma P815.
Cloned genes for human major histocompatibility complex (MHC) class I antigens were introduced by DNA-mediated gene transfer into a high-efficiency transfection recipient (HTR) cell line previously derived from mouse mastocytoma P815. Cell surface expression of HLA-A3, AW24 and CW3 gene products on P815 transfectants was demonstrated by radioimmune assay and by flow cytometry. The human MHC class I gene products were apparently expressed on P815 transfectants in a form recognized by human cytolytic T lymphocytes (CTL). Human CTL generated in unidirectional mixed lymphocyte culture against AW24+ donor lymphocytes clearly lysed P815-HLA-AW24+ transfectant target cells, but not untransfected P815(HTR) controls. Moreover, P815-HLA transfectants could stimulate in vitro a significant alloreactive human CTL response. Lysis of P815-HLA transfectant target cells by human CTL was inhibited by a monoclonal antibody directed against human MHC class I gene products. These mouse cell transfectants may be useful for the study of human T cell responses.
Maryanski
J L
JL
Moretta
A
A
Jordan
B
B
De Plaen
E
E
Van Pel
A
A
Boon
T
T
Cerottini
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001667:Binding, Competitive; D003001:Cloning, Molecular; D003602:Cytotoxicity, Immunologic; D004247:DNA / Q000378:metabolism*; D005802:Genes, MHC Class II; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D012515:Mast-Cell Sarcoma / Q000235:genetics* / Q000276:immunology; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism*; D014162:Transfection
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D004247:DNA