Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF).
cDNA clones having a nucleotide sequence encoding a human monocyte chemotactic and activating factor (MCAF) were isolated and sequenced. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. The amino acid sequence of MCAF showed 25-55% homology with other members of an inducible cytokine family, including macrophage inflammatory protein and some putative polypeptide mediators known as JE, LD78, RANTES and TCA-3. This suggests that MCAF is a member of family of factors involved in immune and inflammatory responses.
Furutani
Y
Y
Research Laboratories, Dainippon Pharmaceutical Co. Ltd., Suita/Osaka, Japan.
Nomura
H
H
Notake
M
M
Oyamada
Y
Y
Fukui
T
T
Yamada
M
M
Larsen
C G
CG
Oppenheim
J J
JJ
Matsushima
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D001685:Biological Factors; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000235:genetics*; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D003062:Codon; D003488:Cyanogen Bromide; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008262:Macrophage Activation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D011498:Protein Precursors; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D001685:Biological Factors; D018932:Chemokine CCL2; D002630:Chemotactic Factors; D003062:Codon; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D011498:Protein Precursors; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D004247:DNA; D003488:Cyanogen Bromide