title: The conditioning effect of optic nerve injury upon axonal regrowth from adult rat retinal ganglion cells explanted in vitro.
abstract: An in vitro assay was used to determine the effects of conditioning nerve lesions on the regeneration of adult rat retinal ganglion cell (RGC) axons from retinal explants. Following the conditioning lesion (CL) of unilateral optic nerve transection, maximal regrowth was seen from RGC explanted from ipsilateral retinae 10 days post-CL. Explants from this group initiated axonal regrowth earlier and a greater percentage regrew axons when compared with explants from normal rats. Axonal regrowth from explants of retinae contralateral to CL was also seen earlier than normal. In further experiments, the effects of both exposure of the optic nerve sheath in the orbit and the incision of the dura without injury to optic nerve axons were studied. The conditioning effect of a dural incision was found to be the same as that of optic nerve transection, whilst exposure of the optic nerve sheath had no conditioning effect on RGC axonal regrowth in vitro.
authors.affiliation: Anatomy Department, United Medical School (Guy's Campus), London, U.K.
authors.forename: A R
authors.initials: AR
authors.lastname: Johnson
authors.forename: N A
authors.initials: NA
authors.lastname: Gregson
authors.forename: C B
authors.initials: CB
authors.lastname: Wigley
authors.forename: M
authors.initials: M
authors.lastname: Berry
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D002478:Cells, Cultured; D009416:Nerve Regeneration; D009900:Optic Nerve / Q000166:cytology; D020221:Optic Nerve Injuries; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*; D013997:Time Factors