Circulating and liver-bound salt-resistant hepatic lipases in the golden hamster.
The serum of male golden hamsters was found to contain a circulating triacylglycerol hydrolase activity (serum lipase). In vitro, the enzyme activity was slightly activated by 1 M NaCl (+20%) and inhibited by rat serum (-29%). The hamster liver contained an enzyme with similar characteristics (liver lipase). This enzyme was released into the circulation after intravenous administration of heparin. Both lipase activities were further characterized and compared. The serum lipase had a pH optimum of 9, which was higher than that of the liver enzyme (pH 8.0). The serum enzyme did not bind to Sepharose-heparin columns in contrast to the liver lipase, which could be eluted from the column with 0.75 M NaCl. A polyclonal antibody preparation raised against the heparin-releasable salt-resistant lipase from rat liver inhibited both the hamster serum enzyme and the liver enzyme completely. The affinity of the antibodies towards the hamster enzymes was lower than the affinity towards the rat liver enzyme, but similar with that towards the hamster enzymes in the serum and the liver. A panel of five monoclonal antibodies raised against the rat enzyme did not bind either of the hamster enzymes. If the hamsters were fed a normal lab chow, the lipase activity in the serum amounted up to 110 +/- 20 mU (mean +/- S.D., n = 16) per ml serum (about 600 mU per animal), the liver contained 200 +/- 41 mU per g tissue (total about 800 mU per animal). In animals fed a cholesterol-enriched diet, the serum activity increased by 82% and the liver activity by 27%.
Jansen
H
H
Department of Internal Medicine III, Erasmus University Rotterdam, The Netherlands.
Lammers
R
R
Baggen
M G
MG
Wouters
N M
NM
Birkenhäger
J C
JC
D000818:Animals; D001769:Blood; D002846:Chromatography, Affinity; D006224:Cricetinae; D006498:Hepatectomy; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008647:Mesocricetus; D012965:Sodium Chloride / Q000494:pharmacology
D012965:Sodium Chloride; D008049:Lipase