title: Rapid prenatal diagnosis of beta thalassemia using DNA amplification and nonradioactive probes.
abstract: We used in vitro DNA amplification by the polymerase chain reaction and nonradioactive probes for prenatal diagnosis of beta thalassemia in Chinese from the Guangdong province. Exact molecular diagnoses were made in all 20 fetuses studied over a 6-month period. We conclude that this method of prenatal diagnosis for beta thalassemia is a viable approach in many parts of the world where this disease is common.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143-0724.
authors.forename: S P
authors.initials: SP
authors.lastname: Cai
authors.forename: C A
authors.initials: CA
authors.lastname: Chang
authors.forename: J Z
authors.initials: JZ
authors.lastname: Zhang
authors.forename: R K
authors.initials: RK
authors.lastname: Saiki
authors.forename: H A
authors.initials: HA
authors.lastname: Erlich
authors.forename: Y W
authors.initials: YW
authors.lastname: Kan
mesh_terms: D001483:Base Sequence; D005260:Female; D005784:Gene Amplification; D006580:Genetic Carrier Screening / Q000379:methods; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D013789:Thalassemia / Q000235:genetics*
chemical_list: D015345:Oligonucleotide Probes