Membrane potential measurements in isolated rat liver plasma membrane vesicles: effect of transmembrane ion concentration gradients.
In isolated basolateral and canalicular rat liver plasma membrane vesicles the membrane potential (measured with DiS-C2 (5] varied with transmembrane concentration gradients of Na+, K+ and Cl- revealing the following ion permeabilities: basolateral vesicles: PNa/PK: 0.76, PCl/PK: 0.45 and canalicular vesicles: PNa/PK: 0.69, PCl/PK: 0.56. The data indicate a permselectivity of PK greater than PNa greater than PCl for both membranes.
Thalhammer
T
T
Institute of General and Experimental Pathology, University of Vienna, Austria.
Peterlik
M
M
Graf
J
J
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability; D002712:Chlorides / Q000378:metabolism*; D004396:Coloring Agents; D004225:Dithiazanine; D008099:Liver / Q000502:physiology*; D008564:Membrane Potentials; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D002712:Chlorides; D004396:Coloring Agents; D004225:Dithiazanine; D012964:Sodium; D011188:Potassium