title: Renal epithelial cell lines (BSC-1, MDCK, LLC-PK1) express 11 beta-hydroxysteroid dehydrogenase activity.
abstract: Renal tissue of several species has been shown to express considerable 11 beta-hydroxysteroid dehydrogenase (11-HSD, EC 1.1.1.146) activity. However, it is uncertain as to which renal cell types exhibit 11-HSD activity. In the present study, we investigated corticosterone metabolism in BSC-1 cells, a continuous renal epithelial cell line derived from the African green monkey (Cercopithecus aethiops). In incubation experiments using 3H-labelled corticosterone and HPLC, we have demonstrated oxidative 11-HSD activity in intact monolayers of BSC-1 cells as well as in BSC-1 cell homogenates. 11-HSD activity in cell homogenates could be stimulated 7-9-fold by the addition of exogenous NADP+ (1 mM). In contrast, no reductive 11-HSD could be detected either in intact cells or in cell homogenates under various experimental conditions which were designed to favor reductive 11-HSD activity. Pilot experiments were performed in cell homogenates from two other renal epithelial cell lines derived from canine (MDCK) and porcine (LLC-PK1) kidney. They also revealed oxidative but no reductive 11-HSD activity. The data provide evidence for an epithelial localization of renal oxidative 11-HSD activity.
authors.affiliation: Institut für Klinische Physiologie, Klinikum Steglitz, Freie Universität Berlin, F.R.G.
authors.forename: C
authors.initials: C
authors.lastname: Korbmacher
authors.forename: W
authors.initials: W
authors.lastname: Schulz
authors.forename: M
authors.initials: M
authors.lastname: König
authors.forename: H
authors.initials: H
authors.lastname: Siebe
authors.forename: I
authors.initials: I
authors.lastname: Lichtenstein
authors.forename: K
authors.initials: K
authors.lastname: Hierholzer
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases; D000818:Animals; D002460:Cell Line; D004285:Dogs; D004848:Epithelium / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis*; D007668:Kidney / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D013552:Swine
chemical_list: D009243:NAD; D009249:NADP; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; D005954:Glucosephosphate Dehydrogenase