raf/myc-infected erythroid cells are restricted in their ability to terminally differentiate.
A comparison was made of the in vitro erythroid colony-forming abilities of v-raf-, v-myc-, and v-raf/v-myc-containing retroviruses. In methylcellulose, v-raf efficiently produced colonies of well-differentiated hemoglobin-synthesizing erythroid cells, whereas v-raf/v-myc-infected erythroid cells were inhibited from terminally differentiating but retained the ability to replicate extensively. In contrast, v-myc was unable to stimulate the formation of erythroid colonies.
Klinken
S P
SP
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Rapp
U R
UR
Morse
H C
HC
D000818:Animals; D002454:Cell Differentiation; D002472:Cell Transformation, Viral; D004920:Erythropoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000382:microbiology*; D066298:In Vitro Techniques; D051379:Mice; D009857:Oncogenes