Microbial degradation of beta-chlorinated four-carbon aliphatic acids.
Alcaligenes sp. strain CC1 is able to grow on several alpha-chlorinated aliphatic acids (2-chlorobutyrate, 2-chloropropionate, and chloroacetate), as well as on the beta-chlorinated four-carbon aliphatic acids trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate as sole carbon and energy sources. Dehalogenation of alpha-chlorinated acids could be measured by using resting cells grown on all the different carbon sources, whereas dehalogenation of beta-chlorinated four-carbon acids could be detected only by using resting cells grown on four-carbon compounds. A constitutive 2-haloacid dehalogenase, which did not show any activity with beta-chlorinated four-carbon acids, was detected in cell extracts. Cell extracts of crotonate-grown cells additionally contained a beta-haloacid dechlorination activity, which acted on trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate and was strictly dependent on coenzyme A, ATP, and Mg2+. Dechlorination of beta-chlorinated four-carbon acids takes place after activation of the acids to their coenzyme A derivatives and seems to be independent of the constitutive 2-haloacid dehalogenase.
Kohler-Staub
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Department of Soil and Environmental Sciences, University of California, Riverside 92521.
Kohler
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D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism*; D000421:Alcaligenes / Q000254:growth & development / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism*; D003437:Crotonates / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D013379:Substrate Specificity
D002087:Butyrates; D003437:Crotonates; D005229:Fatty Acids, Monounsaturated; D006843:Hydrocarbons, Chlorinated; D015094:3-Hydroxyacyl CoA Dehydrogenases; D006867:Hydrolases; C034507:2-haloacid dehalogenase