Comparison of partial amino acid sequences of two protamine 2 variants from stallion sperm. Structural evidence that the variants are products of different genes.
Protamine 1 and two protamine 2 variants were isolated from stallion sperm and separated by acetic acid-urea gel electrophoresis. After electroblotting onto polyvinyldifluoride filters, their amino-terminal amino acid sequences were determined by pulse-liquid peptide sequencing. The sequences of the two protamine 2 variants are homologous but slightly different in length and amino acid composition and indicate for the first time the existence of two different genes for this protamine species.
Pirhonen
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Department of Biochemistry, University of Kuopio, Finland.
Linnala-Kankkunen
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Mäenpää
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PH
D000595:Amino Acid Sequence; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006736:Horses / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011479:Protamines / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013094:Spermatozoa / Q000032:analysis*
D011479:Protamines; C048529:protamine P2