Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones.
Presumptive full-length cDNA clones of 9 of the 12 wound tumor virus double-stranded RNA genome segments were tailored for efficient in vitro expression by a recently described strategy [Z. Xu, J.V. Anzola, and D.L. Nuss (1987) DNA6, 505-513]. In vitro synthesized polypeptides specified by synthetic transcripts corresponding to the tailored cDNAs comigrated in polyacrylamide gels with in vivo synthesized viral-specific polypeptides. This analysis confirmed the functional integrity of the tailored cDNA clones and identified cognate genome segments which encode all five viral non-structural polypeptides as well as four structural polypeptides; two which comprise the capsid, one located in the viral core and one associated with the outer protein coat.
Xu
Z K
ZK
Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
Anzola
J V
JV
Nuss
D L
DL
D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010941:Plant Tumors / Q000382:microbiology*; D010942:Plant Viruses / Q000235:genetics*; D010944:Plants; D010957:Plasmids; D014176:Protein Biosynthesis; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014758:Viral Core Proteins; D014764:Viral Proteins