title: Partial purification of the acyl-CoA elongase of Allium porrum leaves.
abstract: Acyl-CoA elongase has been partially purified from leek (Allium porrum L.) epidermal cells. The microsomal elongase is first solubilized by Triton X-100. The solubilized proteins are then submitted to anion exchange chromatography on DEAE-cellulose and, finally, to gel filtration on Ultrogel 34 AcA. The purification of the elongase activity is accompanied by the enrichment in three major protein bands of 59, 61, and 65 kDa. The partially purified elongase is highly delipidated (about 10 mol lipid/mol of 60- to 65-kDa protein) and phosphatidylserine and phosphatidylethanolamine account respectively for 60 and 40% of the remaining phospholipids. The partially purified elongase retains some activities associated with fatty acid biosynthesis. The overall activity is strongly stimulated by the addition of exogenous lipids. In the presence of a mixture of PS, PE, and PC the C18-CoA elongase activity is increased more than sixfold. The Km value of stearoyl-CoA, in the presence of lipid vesicles, was determined to be 1.7 microM.
authors.affiliation: Institut de Biochimie Cellulaire et Neurochimie, Bordeaux, France.
authors.forename: J J
authors.initials: JJ
authors.lastname: Bessoule
authors.forename: R
authors.initials: R
authors.lastname: Lessire
authors.forename: C
authors.initials: C
authors.lastname: Cassagne
mesh_terms: D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D000490:Allium / Q000201:enzymology*; D002845:Chromatography; D003066:Coenzyme A Ligases / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D007651:Keto Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008861:Microsomes / Q000201:enzymology; D009097:Multienzyme Complexes / Q000302:isolation & purification* / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis
chemical_list: D005227:Fatty Acids; D007651:Keto Acids; D009097:Multienzyme Complexes; D010743:Phospholipids; D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase; D003066:Coenzyme A Ligases