Existence and expression of photoreactivation repair genes in various yeast species.
Photoreactivation repair (Phr) activities in cell extracts of 13 different yeast species were measured by the Haemophilus influenzae transformation assay. Five species including Schizosaccharomyces pombe showed no or low enzymatic activity. In contrast to the other species, chromosomal DNAs of these 5 species did not show detectable hybridization using a DNA fragment of the photolyase PHR1 gene of Saccharomyces cervisiae as a probe even at a low stringency condition. When the PHR1 gene was attached to the 5'-flanking sequence of the iso-1-cytochrome c (CYC-1) gene of S. cerevisiae and introduced into S. pombe cells, the transformants acquired a high Phr activity, indicating that the PHR1 gene alone can provide a Phr-negative species with this repair activity and the light-absorbing cofactor(s) must be present in S. pombe. Our results also demonstrated that the 5'-flanking sequence of the S. cerevisiae CYC-1 gene works in S. pombe as a regulatory element.
Yasui
A
A
Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan.
Eker
A P
AP
Koken
M
M
D015342:DNA Probes; D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics*; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D008027:Light; D008190:Lyases / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014170:Transformation, Genetic; D015003:Yeasts
D015342:DNA Probes; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase