title: Tryptophan 2,3-dioxygenase activity in rat skin.
abstract: We have found an enzyme system that catalyzes the conversion of L-tryptophan to L-kynurenine, presumably via L-formylkynurenine, in soluble and insoluble fractions of rat skin. The enzymatic activity was stimulated by hematin, ascorbate, and catalase, but not by methylene blue. Highest activity was located in the skin of the dorsal posterior region and lowest activity in the abdominal region. The activity in plucked (depilated) skin was only about 25% of that obtained from unplucked (depilated) tissue of the same region. D-Tryptophan, 5-hydroxytryptophan, and tryptamine were not degraded by the skin enzyme and the Km for L-tryptophan determined with the crude enzyme was 1 microM. The decycling activity of rat skin and liver for L-tryptophan began to be stimulated after birth and reached the highest level at 6 weeks. But, 1 week later, most of the skin activity suddenly disappeared and the low level continued at least until 12 weeks. In contrast, the hepatic enzyme did not change so drastically. These findings suggest that an enzyme that catalyzes L-tryptophan to L-kynurenine via L-formylkynurenine is present in rat skin.
authors.affiliation: Department of Biochemistry, School of Hygiene, Fujita-Gakuen Health University, Aichi, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Naito
authors.forename: I
authors.initials: I
authors.lastname: Ishiguro
authors.forename: Y
authors.initials: Y
authors.lastname: Nagamura
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D000367:Age Factors; D000818:Animals; D007737:Kynurenine / Q000096:biosynthesis; D008099:Liver / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000032:analysis / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity; D014364:Tryptophan / Q000378:metabolism; D014366:Tryptophan Oxygenase / Q000378:metabolism*
chemical_list: D007737:Kynurenine; D014364:Tryptophan; D014366:Tryptophan Oxygenase