title: Evidence for a ligation step in the DNA replication of the autonomous parvovirus minute virus of mice.
abstract: Newly replicated DNA of the autonomous parvovirus minute virus of mice was pulse-labeled with 32PO4 during the time of maximal viral DNA replication in highly synchronized A9 cells. The subsequent processing of viral DNA-protein complexes was monitored during a chase period with no label. Several distinct classes of duplex replicative-form and progeny single-stranded DNA molecules were characterized and found to accumulate at different times during infection. Analysis of the terminal structures associated with these various forms provided new insights into the mechanism by which viral DNA replicates and, in particular, suggested that interstrand ligation occurs during this process.
authors.affiliation: Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: S F
authors.initials: SF
authors.lastname: Cotmore
authors.forename: M
authors.initials: M
authors.lastname: Gunther
authors.forename: P
authors.initials: P
authors.lastname: Tattersall
mesh_terms: D000818:Animals; D011088:DNA Ligases / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis / Q000648:ultrastructure; D051379:Mice; D008915:Minute Virus of Mice / Q000502:physiology*; D010321:Parvoviridae / Q000502:physiology*; D011118:Polynucleotide Ligases / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D011088:DNA Ligases; D011118:Polynucleotide Ligases