title: Immunohistochemical characterization of osteoclasts and osteoclast-like cells with monoclonal antibody MB1 on paraffin-embedded tissues.
abstract: In this study we provide evidence that MB1, a newly developed monoclonal antibody which reacts with B lymphocytes and a proportion of T cells and monocytes, can be successfully used for the direct immunohistochemical identification of osteoclasts on paraffin-embedded surgical specimens. The antigen(s) recognized by MB1 is present at high density in the cytoplasm of osteoclasts of fetal bone and in the multinucleated cells of human giant cell tumour of bone (osteoclastoma), but is weakly expressed or absent in the giant cells of granulomas. MB1 is thus proposed as a new immunohistochemical marker for osteoclasts on paraffin-embedded material.
authors.affiliation: Istituto di Anatomia Patologica, University of Verona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Chilosi
authors.forename: E
authors.initials: E
authors.lastname: Gilioli
authors.forename: M
authors.initials: M
authors.lastname: Lestani
authors.forename: F
authors.initials: F
authors.lastname: Menestrina
authors.forename: L
authors.initials: L
authors.lastname: Fiore-Donati
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000911:Antibodies, Monoclonal; D001859:Bone Neoplasms / Q000473:pathology*; D001842:Bone and Bones / Q000166:cytology / Q000196:embryology; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism; D005870:Giant Cell Tumors / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D010010:Osteoclasts / Q000166:cytology* / Q000201:enzymology / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D000135:Acid Phosphatase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4