title: Persistence at low temperature of the P beta' ripple in dipalmitoylphosphatidylcholine multilamellar vesicles containing either glycosphingolipids or cholesterol.
abstract: The disappearance and reappearance of the P beta' ripple in multilamellar liposomes of dipalmitoylphosphatidylcholine (DPPC) has been examined by freeze-etch electron microscopy. The presence of less than 10 mol% of various glycosphingolipids or cholesterol in the liposomes markedly increases the time required for ripple disappearance when the vesicles are cooled from 38 degrees C to 30 degrees C, as compared to the pure phospholipid. Once the ripples have begun to disappear in the two-component vesicles, they do not uniformly reappear until the system is heated above the main transition of DPPC and allowed to cool into the pretransition region. These results suggest that the long time for ripple disappearance in the two-component systems reflects a slow molecular reorganization process which occurs when the systems are forced to change from the P beta' gel to the L beta' gel by a temperature downshift.
authors.affiliation: Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908.
authors.forename: P
authors.initials: P
authors.lastname: Rock
authors.forename: T E
authors.initials: TE
authors.lastname: Thompson
authors.forename: T W
authors.initials: TW
authors.lastname: Tillack
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol / Q000494:pharmacology*; D003080:Cold Temperature; D005613:Freeze Etching; D005677:G(M1) Ganglioside / Q000494:pharmacology; D006028:Glycosphingolipids / Q000494:pharmacology*; D008081:Liposomes; D008854:Microscopy, Electron
chemical_list: D006028:Glycosphingolipids; D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D002784:Cholesterol