title: Acrylamide-induced increases in deposition of axonally transported glycoproteins in rat sciatic nerve.
abstract: The axonal transport of proteins, glycoproteins, and gangliosides in sensory neurons of the sciatic nerve was examined in adult rats exposed to acrylamide via intraperitoneal injection (40 mg/kg of body weight/day for nine consecutive days). The L5 dorsal root ganglion was injected with either [35S]methionine to label proteins or [3H]glucosamine to label, more specifically, glycoproteins and gangliosides. At times ranging from 2 to 6 h later, the sciatic nerve and injected ganglion were excised and radioactivity in consecutive 5-mm segments determined. In both control and acrylamide-treated animals, outflow profiles of [35S]methionine-labeled proteins showed a well defined crest which moved down the nerve at a rate of approximately 340 mm/day. Similar outflow profiles and transport rates were seen for [3H]glucosamine-labeled glycoproteins in control animals. However, in animals treated with acrylamide, the crest of transported labeled glycoprotein was severely attenuated as it moved down the nerve. This finding suggests that in acrylamide-treated animals, axonally transported glycoproteins were preferentially transferred (unloaded or exchanged against unlabeled molecules) from the transport vector to stationary axonal structures. We also examined the clearance of axonally transported glycoproteins distal to a ligature on the nerve. The observed impairment of clearance in acrylamide-treated animals relative to controls is supportive of the above hypothesis. Acrylamide may directly affect the mechanism by which axonally transported material is unloaded from the transport vector. Alternatively, the increased rate of unloading might reflect an acrylamide-induced increase in the demand for axonally transported material.
authors.affiliation: Biological Sciences Research Center, University of North Carolina, Chapel Hill 27599.
authors.forename: G J
authors.initials: GJ
authors.lastname: Harry
authors.forename: J F
authors.initials: JF
authors.lastname: Goodrum
authors.forename: T W
authors.initials: TW
authors.lastname: Bouldin
authors.forename: A D
authors.initials: AD
authors.lastname: Toews
authors.forename: P
authors.initials: P
authors.lastname: Morell
mesh_terms: D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism*; D008026:Ligation; D046911:Macromolecular Substances; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000178:Acrylamides; D006023:Glycoproteins; D046911:Macromolecular Substances; D020106:Acrylamide