title: Collagen degradation in the rabbit skin during short-term tissue culture.
abstract: Full thickness rabbit skin explants were cultured on plastic dish for 1 week and the sequential morphological changes were examined daily by light and electron microscopy. During the cultured period, bundles of dermal collagen fibres gradually loosened and were removed from the upper dermis and from the cut margin of the explant, which was covered by a sheet of migrating epidermal cells. In these areas, cells containing phagocytosed collagen fibrils were observed from the 3rd day to the end of the culture period. These cells containing phagocytosed collagen fibrils included dermal fibroblasts and macrophages, epidermal keratinocytes and endothelial cells lining blood vessels. The presence of acid phosphatase activity in vacuoles containing the collagen fibrils suggested that intracellular degradation of collagen was occurring. In addition, extracellular collagen degradation was recognized around fibroblasts and beneath the migrating epidermis by the high collagenolytic activity at these sites. These findings suggest that both intra- and extracellular collagen degradation may participate in collagen removal from dermal connective tissue in cultured skin explants.
authors.affiliation: Department of Pathology Tokyo Medical College, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Asuwa
mesh_terms: D000818:Animals; D002465:Cell Movement; D003094:Collagen / Q000378:metabolism*; D046508:Culture Techniques; D004730:Endothelium, Vascular / Q000166:cytology / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D008264:Macrophages / Q000648:ultrastructure; D008854:Microscopy, Electron; D010587:Phagocytosis; D011817:Rabbits; D012867:Skin / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D003094:Collagen