Coprecipitation of enzymes with water soluble starch--an alternative to freeze-drying.
Krill proteases were prepared in solid form from a partially purified extract by coprecipitation of the enzymes with water-soluble starch in an organic solvent at 22 degrees C. The precipitation did not affect the activity of the enzymes. The recovery of proteolytic activity was 100%. The thermostability of the krill proteases increased when incorporated in the starch precipitate. No reduction in enzymatic activity could be seen after storage at +50 degrees C for 99 days. After milling the coprecipitate could be dispensed. The enzyme preparation consisted of irregular needle-shaped particles. This simple precipitation technique offers an alternative to freeze-drying or spray-drying.
Randén
L
L
Pharmacia-Leo Therapeutics AB, Uppsala, Sweden.
Nilson
J
J
Edman
P
P
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004800:Enzymes, Immobilized / Q000302:isolation & purification*; D005612:Freeze Drying; D006358:Hot Temperature; D010316:Particle Size; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D010933:Plankton / Q000201:enzymology*; D012995:Solubility; D013213:Starch
D004800:Enzymes, Immobilized; D013213:Starch; D010447:Peptide Hydrolases