title: Characterization of rabbit lactate dehydrogenase-M and lactate dehydrogenase-H cDNAs. Control of lactate dehydrogenase expression in rabbit muscle.
abstract: Two cDNA clones were isolated, one corresponding to the mRNA coding for lactate dehydrogenase-M (LDH-M), the other to the mRNA coding for lactate dehydrogenase-H (LDH-H). The cDNA inserts consist of the entire open reading frame for LDH-M and a partial sequence, from amino acid 117 to 332, for LDH-H. Using these two clones as probes we demonstrate that: (a) the abundance of mRNA is muscle-type dependent; (b) the ratio M/H subunit for protein and mRNA is well related in the muscles studied; and (c) the M + H mRNA level is not relative to the total LDH activity.
authors.affiliation: Laboratoire de Biochimie, Universite Blaise Pascal-Clermont-Ferrand, Aubiere, France.
authors.forename: C
authors.initials: C
authors.lastname: Sass
authors.forename: M
authors.initials: M
authors.lastname: Briand
authors.forename: S
authors.initials: S
authors.lastname: Benslimane
authors.forename: M
authors.initials: M
authors.lastname: Renaud
authors.forename: Y
authors.initials: Y
authors.lastname: Briand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D009132:Muscles / Q000502:physiology*; D011817:Rabbits / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004247:DNA; D007770:L-Lactate Dehydrogenase