Squalene epoxide cyclase and microemulsion.
Squalene epoxide cyclase was extracted from microsomal preparations of rat liver using anionic, cationic and non-ionic microemulsions. The anionic microemulsion was the best with respect to protein solubilisation, extracted cyclase activity and stability of this activity over time. The activity assay showed cyclase activity to be higher in anionic microemulsion than in buffer in the presence of surfactant. Calcium chloride in the anionic microemulsion had a stabilising effect and less total protein seemed to be extracted.
Lee
K M
KM
UA-31, Institut de Chimie, Université Louis Pasteur, Strasbourg, France.
Duriatti
A
A
Schuber
F
F
Biellmann
J F
JF
D000818:Animals; D002021:Buffers; D002122:Calcium Chloride / Q000494:pharmacology; D004655:Emulsions; D007535:Isomerases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology*; D010105:Oxygenases / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats
D002021:Buffers; D004655:Emulsions; C022663:squalene epoxidase-cyclase; D010105:Oxygenases; D007535:Isomerases; D002122:Calcium Chloride