title: Exocytotic release of neurotransmitter substances from nerve endings in the taste buds of rat circumvallate papillae.
abstract: Exocytotic release of neurotransmitters from nerve endings was demonstrated ultrastructurally in the taste buds of rat circumvallate papillae by stimulation of high K+ and Ca2+ Ringer perfusion and application of tannic acid-Ringer incubation (TARI) method. Omega-shaped images of large cored vesicles and small clear vesicles, indicating exocytotic release of their contents, were found only in the non-synaptic sites. Occasionally exocytosis occurred at sites facing other nerve fibers. Many coated pits were also seen, which presumably represent membrane retrieval at a later stage of exocytosis. It is likely that the taste buds receive more than one type of innervation.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Endo
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003034:Coated Pits, Cell-Membrane / Q000502:physiology / Q000648:ultrastructure; D005089:Exocytosis / Q000187:drug effects; D047348:Hydrolyzable Tannins; D007552:Isotonic Solutions; D008297:Male; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000378:metabolism* / Q000648:ultrastructure; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D000077331:Ringer's Solution; D013569:Synapses / Q000648:ultrastructure; D013650:Taste Buds / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D047348:Hydrolyzable Tannins; D007552:Isotonic Solutions; D018377:Neurotransmitter Agents; D000077331:Ringer's Solution; D011188:Potassium; D002118:Calcium