Constitutive and Aroclor 1254-induced hepatic glutathione S-transferase, peroxidase and reductase activities in genetically inbred mice.
1. Constitutive and Aroclor 1254-induced hepatic glutathione (GSH) S-transferases, GSH peroxidase and GSH reductase activities were determined in 12 strains of 8-10 week-old inbred male mice. 2. The constitutive GSH S-transferase activity varied from 2.5 (SJL/JCR) to 8.9 (C57BL/6N) mumol/min/mg protein and the corresponding values for the Aroclor 1254-treated mice were in the range of 7.1-23.0 mumol/min/mg protein. Aroclor 1254 significantly induced GSH S-transferase activity in all mice, however, significant interstrain differences were found in inducibility. 3. Aroclor 1254-treatment caused a 4.2-fold induction of GSH S-transferase in NFS/NCR but only a 1.4-fold increase in AKR/NCR mice. Aroclor 1254 significantly induced GSH reductase in all strains studied while GSH peroxidase activity decreased in these mice. 4. The range of hepatic GSH levels in control and Aroclor 1254-treated mice was relatively narrow for both groups (6.59-11.25 microM/g wet tissue).
Makary
M
M
Veterinary Physiology and Pharmacology and Pathology, Texas A&M University, College Station 77843.
Kim
H L
HL
Safe
S
S
Womack
J
J
Ivie
G W
GW
D000818:Animals; D001140:Aroclors / Q000494:pharmacology*; D020111:Chlorodiphenyl (54% Chlorine); D004790:Enzyme Induction; D005979:Glutathione Peroxidase / Q000096:biosynthesis*; D005980:Glutathione Reductase / Q000096:biosynthesis*; D005982:Glutathione Transferase / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*
D001140:Aroclors; D020111:Chlorodiphenyl (54% Chlorine); D011078:Polychlorinated Biphenyls; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase