Plasma catecholamine concentrations in rainbow trout (Salmo gairdneri) at rest and after anesthesia and surgery.
The effects of surgery and anesthesia on concentrations of plasma epinephrine (E), norepinephrine (NE), and dopamine (DA) were investigated in rainbow trout fitted with dorsal aorta cannulae. Baseline catecholamines (CA) concentrations, established in resting rainbow trout, were 1.55 +/- 0.90 pmol/ml (mean +/- SD) for E, 2.07 +/- 1.26 for NE, and 1.33 +/- 0.87 for DA. These values were based on the pooled analyses of five individual fish taken over seven different sampling periods. The E:NE ratio in resting fish was always less than 1.0. In a second experiment, fish were subjected to dorsal aorta cannulation and sequential blood samples were taken immediately after surgery, and 6, 24, and 48 hr later. Plasma E concentrations were 36 times greater than baseline values in the first sample; NE was 15 times greater and DA was 41 times greater. After surgery, plasma concentrations of all CAs fell rapidly but values were still higher than baseline 6 hr after surgery, then were near baseline at 24 and 48 hr after surgery. The E:NE ratio was about 3.0 immediately after surgery, dropped to 1.8 at 6 hr, and was about 1.0 at 24 and 48 hr. In a third experiment, plasma CAs were determined in a group of five animals anesthetized with tricaine methanesulfonate (100 mg/ml) to advanced anesthesia, and then allowed to recover in flowing well water over a 12-hr observation period. Plasma E and NE concentrations in the fish during early anesthesia (1.14 +/- 0.14 min) were not significantly different from preanesthesia values.(ABSTRACT TRUNCATED AT 250 WORDS)
Gingerich
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United States Fish and Wildlife Service, National Fisheries Research Center, La Crosse, Wisconsin 54602.
Drottar
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D062365:Aminobenzoates / Q000494:pharmacology*; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D002395:Catecholamines / Q000097:blood*; D004298:Dopamine / Q000097:blood; D004837:Epinephrine / Q000097:blood; D009638:Norepinephrine / Q000097:blood; D012146:Rest; D012487:Salmonidae / Q000097:blood*; D013312:Stress, Physiological / Q000097:blood*; D013514:Surgical Procedures, Operative / Q000009:adverse effects*; D014337:Trout / Q000097:blood*
D062365:Aminobenzoates; D000777:Anesthetics; D002395:Catecholamines; C003636:tricaine; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine