Thymidine suicide and hydroxyurea kill ratios accurately reflect the proliferative status of stem cells (CFU-S).
We have previously found that on average 30% of hematopoietic stem cells (CFU-S) are in the S phase, which is at least three times the value published by others. Therefore, it seemed desirable to investigate the reliability of the methods used to measure the percentage of CFU-S in S phase. Various modifications of the [3H]thymidine suicide were tested and it could be demonstrated that results were not affected by them. Furthermore, results obtained with the [3H]thymidine suicide were compared to those obtained with methods utilizing hydroxyurea to kill CFU-S in S phase. The [3H]thymidine- and hydroxyurea-based methods gave parallel results. The parallel behavior validated all of these methods as reliable indicators of the turnover of CFU-S and presumably other stem cell populations.
Necas
E
E
Department of Pathophysiology, Faculty of General Medicine, Charles University, Prague, Czechoslovakia.
Znojil
V
V
Frindel
E
E
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival; D004261:DNA Replication / Q000187:drug effects*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013936:Thymidine / Q000494:pharmacology*
D013936:Thymidine; D006918:Hydroxyurea