Metabolic profiling of urinary organic acids by single and multicolumn capillary gas chromatography.
High-resolution gas chromatography (HRGC) and gas chromatography/mass spectrometry (GC/MS) are the techniques of choice to determine the retention indices of more than 200 organic acids as their trimethylsilyl (TMS) or oxime-trimethylsilyl derivatives. Several types of apolar and semipolar fused-silica capillary columns (OV-1, SE-52, and OV-1701), used to analyze and separate organic acids isolated from urine samples, are evaluated.
Lefevere
M F
MF
Laboratoria voor Medische Biochemie en voor Klinische Analyse, Gent, Belgium.
Verhaeghe
B J
BJ
Declerck
D H
DH
Van Bocxlaer
J F
JF
De Leenheer
A P
AP
De Sagher
R M
RM
D000143:Acids / Q000652:urine*; D000328:Adult; D002675:Child, Preschool; D002849:Chromatography, Gas; D005410:Flame Ionization; D006801:Humans; D007223:Infant
D000143:Acids