Restriction fragment length polymorphisms of horse class II MHC genes observed using various human alpha- and beta-chain cDNA probes.
Genomic DNA isolated from 20 horses was digested with up to six restriction endonucleases and subjected to southern blot hybridization analysis using various human class II alpha- and beta-chain cDNA probes. A high degree of restriction fragment length polymorphism (RFLP) was found for the DQ alpha, DP beta, DQ beta and DR beta probes, about 20 polymorphic bands being detected for each. DR alpha showed 2-4 polymorphic bands, whereas no evidence for DP alpha-like genes was found. A number of correlations of RFLPs with individual alloantisera were apparent.
Hänni
K
K
Klinik fur Nutztiere und Pferde, Institute of Animal Husbandry, University of Berne, Switzerland.
Hesford
F
F
Lazary
S
S
Gerber
H
H
D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D004247:DNA; D015342:DNA Probes; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006736:Horses / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D000949:Histocompatibility Antigens Class II; D004247:DNA