title: The N-terminal domain of the human androgen receptor is encoded by one, large exon.
abstract: Using specific cDNA hybridization probes, the first coding exon of the human androgen receptor gene was isolated from a genomic library. The exon contained an open reading frame of 1586 bp, encoding an androgen receptor amino-terminal region of 529 amino acids. The deduced amino acid sequence was characterized by the presence of several poly-amino acid stretches of which the long poly-glycine stretch (16 residues) and the poly-glutamine stretch (20 residues) were most prominent. Androgen receptor cDNAs from different sources contained information for poly-glycine stretches of variable size (23 and 27 residues, respectively). The androgen receptor amino-terminal domain was found to be hydrophilic and have a net negative charge. Combined with the previously described, partially overlapping cDNA clone 7A2M27 (Trapman et al. (1988) Biochem. Biophys. Res. Commun. 153, 241-248), the complete human androgen receptor was deduced to have a size of 910 amino acids.
authors.affiliation: Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: P W
authors.initials: PW
authors.lastname: Faber
authors.forename: G G
authors.initials: GG
authors.lastname: Kuiper
authors.forename: H C
authors.initials: HC
authors.lastname: van Rooij
authors.forename: J A
authors.initials: JA
authors.lastname: van der Korput
authors.forename: A O
authors.initials: AO
authors.lastname: Brinkmann
authors.forename: J
authors.initials: J
authors.lastname: Trapman
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D011944:Receptors, Androgen / Q000032:analysis / Q000235:genetics*
chemical_list: D011944:Receptors, Androgen; D004247:DNA