A second transport ATPase gene in Saccharomyces cerevisiae.
A second transport ATPase gene from Saccharomyces cerevisiae has been identified by hybridization to a PMA1 probe and sequenced. The gene called PMA2 encodes a polypeptide of Mr = 102,157, which, with the exception of the 144 amino-terminal residues, is highly homologous to the structural gene PMA1 for the H+-ATPase. It is localized on the chromosome XVI at 16.7 centimorgan from gal4 and is not essential for haploid growth. Comparison between the upstream, noncoding DNA regions of PMA1 and PMA2 indicates that the two genes are controlled differently. The extensive amino acid sequence homology with the fungal H+-ATPases described so far indicates that the PMA2-encoded protein is also able to function as a H+ pump. This is supported by the observation that in pma1 mutants with reduced plasma membrane ATPase activity, disruption of the PMA2 gene confers the ability to grow under alkaline pH conditions. Slower development of diploids is also observed on normal minimal medium after bilateral disruption of PMA2 in the two parents.
Schlesser
A
A
Unité de Biochimie Physiologique, Université de Louvain, Louvain-la-Neuve, Belgium.
Ulaszewski
S
S
Ghislain
M
M
Goffeau
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D005810:Multigene Family; D006180:Proton-Translocating ATPases / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
D004251:DNA Transposable Elements; D006180:Proton-Translocating ATPases