Structure of the ARO3 gene of Saccharomyces cerevisiae.
In Saccharomyces cerevisiae, the genes ARO3 and ARO4 encode isoenzymes of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase. Both genes are derepressed seven-fold under the general control of amino acid biosynthesis. A previously isolated 1.7 kb fragment containing the ARO3 gene and the 5'- and 3'-flanking regions was sequenced. The endpoints of the ARO3 transcript coding for a 370 amino acid protein were mapped by primer extension experiments and S1 nuclease digestion. Promoter elements involved in transcription initiation and responsible for the strong general control derepression response are discussed.
Paravicini
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Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, Zürich, Switzerland.
Braus
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Hütter
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D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000235:genetics*; D000446:Aldehyde-Lyases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D005796:Genes; D005800:Genes, Fungal; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D010649:Phenylalanine / Q000502:physiology; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases
D004271:DNA, Fungal; D007527:Isoenzymes; D010649:Phenylalanine; D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D000446:Aldehyde-Lyases