The irritancy and allergenicity of 2-n-octyl-4-isothiazolin-3-one (Skane M-8), with recommendations for patch test concentration.
Preparations of 2-n-octyl-4-isothiazolin-3-one (Skane M-8) at 1000 p.p.m. a.i. and 500 p.p.m. a.i. in pet. induced allergy in a cumulative insult human patch test. Challenge patch testing using 250 p.p.m. a.i. Skane M-8 elicited allergy in 6 out of 6 sensitized individuals. Sensitized individuals did not cross react with Kathon biocide, (Kathon CG), a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one. All pretrolatum preparations to which Tween 85 was added at 2.5% produced mild irritation in cumulative insult patch tests, but no irritation was observed with Tween 85 at 0.625%. We conclude that Skane M-8, 250 p.p.m. a.i. with Tween 85 0.625% in petrolatum is appropriate for patch testing for Skane M-8 allergy.
Emmett
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EA
Centre for Occupational Environmental Health, School of Hygiene and Public Health, John Hopkins University.
Ng
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SK
Levy
M A
MA
Moss
J N
JN
Morici
I J
IJ
D000328:Adult; D000818:Animals; D003877:Dermatitis, Contact / Q000175:diagnosis / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006801:Humans; D010328:Patch Tests / Q000379:methods*; D011136:Polysorbates / Q000276:immunology; D012882:Skin Tests / Q000379:methods*; D013844:Thiazoles / Q000008:administration & dosage / Q000276:immunology*; D013997:Time Factors
D011136:Polysorbates; D013844:Thiazoles; C035331:2-n-octyl-4-isothiazolin-3-one; C037057:Kathon 886