Protein-DNA conjugates produced by UV irradiation and their use as probes for hybridization.
Several proteins have been crosslinked to DNA by low dose uv irradiation. The principle of the method is based on an efficient and fast radiation induced reaction of amino acid residues with DNA at low pH. The method seems to be of general applicability for crosslinking proteins to DNA in a very simple one step procedure. Some of such DNA-protein conjugates have been used as probes for hybridization experiments. DNA-protein A probes were found to be most useful.
Czichos
J
J
Medizinisch Technisches Laboratorium, Denagen, Heidelberg, FRG.
Köhler
M
M
Reckmann
B
B
Renz
M
M
D015139:Blotting, Southern; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial / Q000528:radiation effects; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000528:radiation effects*; D011506:Proteins / Q000528:radiation effects*; D013205:Staphylococcal Protein A / Q000528:radiation effects; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D011506:Proteins; D013205:Staphylococcal Protein A