Translational regulation of the differential synthesis of nonmuscle tropomyosin isoforms.
Regulation of the differential synthesis of tropomyosin (TM) isoforms in chicken embryo fibroblasts was studied. Cell-free translation experiments with total RNA and Northern blot analyses of TM mRNAs from fractionated polysomes were performed. Unlike the major isoforms, mRNAs encoding at least one of the minor isoforms were only partially associated with the heavy polysomes. Thus, these experiments revealed that translational regulation plays a role in controlling the relative amounts of major and minor isoforms in the cell.
Hendricks
M B
MB
Hutchinson Cancer Research Center, Seattle, Washington 98104.
D000818:Animals; D002642:Chick Embryo; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D008970:Molecular Weight; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification; D014335:Tropomyosin / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D014335:Tropomyosin