Structure of T cell antigen receptor beta chain in synovial fluid cells from patients with rheumatoid arthritis.
We attempted to identify a clonal proliferation of T cells from synovial fluid samples from patients with rheumatoid arthritis, using techniques of restriction fragment length polymorphism. We used probes for the beta chain of the T cell receptor to analyze restriction fragments prepared from the genomic DNA of synovial fluid mononuclear cells from 10 patients and synovial fluid T cell preparations from 5 additional patients. The results demonstrated unarranged (germline) T cell receptor gene fragments of DNA in all cell preparations, indicating the lack of clonality of rheumatoid arthritis synovial fluid T cells.
Keystone
E C
EC
Department of Medicine, Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Canada.
Minden
M
M
Klock
R
R
Poplonski
L
L
Zalcberg
J
J
Takadera
T
T
Mak
T W
TW
D001172:Arthritis, Rheumatoid / Q000276:immunology*; D002999:Clone Cells; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D013582:Synovial Fluid / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology