Role of macrophages as modulators but not as autonomous accessory cells in primary antibody response.
The role of macrophages (M phi) in the in vitro primary antibody response of murine lymphocytes to sheep erythrocytes was investigated. Peritoneal M phi were activated to express Ia antigens either in vitro or in vivo. Nonactivated Ia- M phi were also examined. We observed that only Ia- M phi but also Ia+ M phi failed to trigger the antibody response, in contrast with splenic dendritic cells (DC) which served as potent and autonomous accessory cells, but that M phi modulated the level of response which was dependent primarily on the DC content of culture. The modulation appeared to incline to suppression rather than enhancement, when M phi were allowed to remain throughout the culture period for 4 days. A highly enhancing capacity of M phi, however, could be revealed by removing M phi 2 days after the initiation of culture, indicating that M phi exerted their suppressive effect more strongly in the late phase than in the early phase of in vitro antibody response. The modulatory activity seemed higher in Ia+ M phi than in Ia- M phi.
Komatsubara
S
S
Hirayama
Y
Y
Inaba
K
K
Naito
K
K
Yoshida
K
K
Kawai
J
J
Muramatsu
S
S
D000818:Animals; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000145:classification / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology
D001789:Blood Group Antigens