Dominance of resistance to the alkylating agent 1,2:5,6-dianhydrogalactitol in P388 mouse lymphoma hybrid cells.
Cultured P388/S mouse lymphoma cells resistant to 5-bromodeoxyuridine (BUdR) and deficient in thymidine kinase (TK-) were fused with P388/DAG cells resistant to 1,2:5,6-dianhydrogalactitol (DAG), an anticancer alkylating agent, and to 6-thioguanine (6-TG) and deficient in hypoxanthine phosphoribosyl-transferase (HPRT-). Sensitivity to DAG in the hybrid line was very close to that in the P388/DAG line, which means that resistance to DAG was inherited in a quasi-dominant manner. Hybrid cells showed cross-resistance, similar to that of the DAG-resistant line, to two other hexitols, dibromodulcitol (DBD) and disuccinyldianhydrogalactitol (DisuDAG).
Pályi
I
I
Research Institute of Oncopathology, National Institute of Oncology, Budapest, Hungary.
Bence
J
J
Szikla
K
K
Hullán
L
L
D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003961:Dianhydrogalactitol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D006822:Hybrid Cells; D008223:Lymphoma / Q000235:genetics / Q000473:pathology; D051379:Mice; D008811:Mice, Inbred DBA; D013402:Sugar Alcohols / Q000494:pharmacology*; D013937:Thymidine Kinase / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000477:Alkylating Agents; D013402:Sugar Alcohols; D003961:Dianhydrogalactitol; D013937:Thymidine Kinase