title: Simultaneous recording of dorsal raphe unit activity and serotonin release in the striatum using voltammetry in awake, behaving cats.
abstract: Simultaneous recordings of unit activity in the dorsal raphe nucleus (RD) and serotonin (5HT) release in the striatum were made in the cat. Unit recordings were made using Formvar-coated 32 microns diameter nichrome wires. 5HT release was measured using linear sweep voltammetry with semi-differentiation using electrodes prepared from Teflon-coated 32-gauge stainless steel wire filled with carbon paste and Ag/AgCl electrodes and 27-gauge stainless steel needles as reference and auxiliary electrodes, respectively. The working electrodes were scanned at a rate of 10 mV/s over the range of -0.1 to +0.5 V every 5 minutes using a BAS CV37 voltammograph. During REM sleep RD unit activity was decreased 94% from quiet waking (QW) baseline, while the voltammetric response was decreased by only 57%. Chloral hydrate anesthesia decreased RD unit activity by 18% from QW while the voltammetric response was decreased by 39%. LSD decreased RD unit activity by 50% from QW, but the voltammetric response was decreased by 88%. P-chlorophenylalanine produced no significant change in RD unit activity but decreased the voltammetric response by 82%. These data suggest that RD unit activity and 5HT release often differed dramatically.
authors.forename: M E
authors.initials: ME
authors.lastname: Trulson
mesh_terms: D000818:Animals; D002415:Cats; D002697:Chloral Hydrate / Q000494:pharmacology; D002940:Circadian Rhythm; D003342:Corpus Striatum / Q000378:metabolism*; D004594:Electrophysiology / Q000379:methods; D010134:Fenclonine / Q000494:pharmacology; D008238:Lysergic Acid Diethylamide / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D011903:Raphe Nuclei / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism*
chemical_list: D012701:Serotonin; D002697:Chloral Hydrate; D008238:Lysergic Acid Diethylamide; D010134:Fenclonine