title: Significance of the transmural diminution in regional hydrogen ion production after repeated coronary artery occlusions.
abstract: Previous studies have revealed that the regional accumulation of ischemic metabolites including hydrogen ion (H+) and PCO2 diminish after repeated occlusions. We postulated that this diminution reflects a blunted metabolic response that is related to the severity of ischemic injury and, hence, may be most pronounced in subendocardial (ENDO) regions. To investigate this hypothesis, the left anterior descending coronary artery was serially occluded three times in 51 dogs for a period of either 3 minutes (n = 15), 5 minutes (n = 18), or 15 minutes (n = 18). Each occlusion was separated by 45 minutes of reperfusion. Myocardial [H+] was measured in the endomyocardium and in the epimyocardium of the ischemic anterior wall by use of miniature pH glass electrodes. Accumulation of H+ during occlusion (delta [H+]) in the ENDO region was significantly less during the second occlusion when compared with the first occlusion (3-minute occlusions: 28.2 +/- 3.7 nM/l vs. 39.4 +/- 5.4 nM/l, p less than 0.002; 5-minute occlusions: 49.8 +/- 5.0 nM/l vs. 72.1 +/- 6.5 nM/l, p less than 0.0002; 15-minute occlusions: 132.3 +/- 14.6 nM/l vs. 225.6 +/- 27.7 nM/l, p less than 0.0003). A similar trend was noted for delta [H+] in the subepicardial (EPI) regions. During occlusion, the rise in [H+] occurred sooner, and delta [H+] was consistently greater in the ENDO when compared with the EPI regions (p less than 0.05). Regional myocardial blood flow did not change during the three occlusions, indicating that the diminution in H+ accumulation stemmed from a decrease in H+ production and not from an increase in collateral flow. The decrement in H+ accumulation between the first and second occlusions (delta [H+]1-delta [H+]2) 1) was greater in the ENDO than in the EPI regions (p less than 0.05); 2) correlated with the duration of occlusion (ENDO: r = 0.66, p less than 0.001; EPI: r = 0.82, p less than 0.0001); and 3) was related to the impairment of anterior wall systolic shortening after the first reperfusion period. These findings suggest that the diminution in H+ production that follows serial coronary occlusions reflects a blunted metabolic response that is related to both the duration of ischemia and the degree of systolic dysfunction. Moreover, though attenuation of ischemic metabolite production occurs transmurally, it is most pronounced in the deep ENDO regions.
authors.affiliation: Brockton and West Roxbury Veterans Administration Medical Center, MA 02132.
authors.forename: K G
authors.initials: KG
authors.lastname: Warner
authors.forename: S F
authors.initials: SF
authors.lastname: Khuri
authors.forename: W
authors.initials: W
authors.lastname: Marston
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.forename: M D
authors.initials: MD
authors.lastname: Butler
authors.forename: S N
authors.initials: SN
authors.lastname: Assousa
authors.forename: A J
authors.initials: AJ
authors.lastname: Saad
authors.forename: S Y
authors.initials: SY
authors.lastname: Siouffi
authors.forename: P T
authors.initials: PT
authors.lastname: Lavin
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D003326:Coronary Circulation; D003327:Coronary Disease / Q000378:metabolism* / Q000503:physiopathology; D004285:Dogs; D004699:Endocardium / Q000378:metabolism; D005898:Glass; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008839:Microelectrodes; D009206:Myocardium / Q000378:metabolism*; D012008:Recurrence; D013599:Systole; D013997:Time Factors
chemical_list: D006859:Hydrogen