Cerebrospinal fluid analysis of rabid and vaccinia-rabies glycoprotein recombinant, orally vaccinated raccoons (Procyon lotor).
Cerebrospinal fluid obtained from clinically normal free-ranging raccoons was analyzed and compared with CSF obtained from raccoons vaccinated orally with vaccinia-rabies glycoprotein (V-RG) recombinant virus vaccine and subsequently challenged peripherally with street rabies virus, and CSF from naive, rabies virus challenge-exposed control raccoons. Significant differences were not found in CSF of free-ranging or V-RG recombinant virus vaccine recipient raccoons, and there was no evidence of CNS invasion by V-RG virus. The CSF of naive, rabies challenge-exposed control raccoons contained high numbers of lymphocytes and monocytes, compatible with rabies virus encephalitis. Although V-RG orally vaccinated challenge-exposed raccoons were protected from lethal rabies virus infection, a mild lymphocytic pleocytosis was evident at 90 days after challenge exposure.
Hanlon
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Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Ziemer
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Hamir
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Rupprecht
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D000818:Animals; D006023:Glycoproteins / Q000276:immunology; D007958:Leukocyte Count / Q000662:veterinary; D011818:Rabies / Q000134:cerebrospinal fluid / Q000662:veterinary*; D011819:Rabies Vaccines / Q000276:immunology*; D011821:Raccoons / Q000134:cerebrospinal fluid*; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000276:immunology
D006023:Glycoproteins; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic