title: Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp.
abstract: The nucleotide sequence of a 2.1-kilobase-pair fragment containing the Streptomyces choA gene, which codes a secreted cholesterol oxidase, was determined. A single open reading frame encodes a mature cholesterol oxidase of 504 amino acids, with a calculated Mr of 54,913. The leader peptides extend over 42 amino acids and have the characteristics of a signal sequence, including basic amino acids near the amino terminus and a hydrophobic core near the signal cleavage site. Analyses of the total amino acid composition and amino acid sequencing of the first 21 amino acids from the N terminus of the purified extracellular enzyme agree with the values deduced from nucleotide sequencing data.
authors.affiliation: Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishizaki
authors.forename: N
authors.initials: N
authors.lastname: Hirayama
authors.forename: H
authors.initials: H
authors.lastname: Shinkawa
authors.forename: O
authors.initials: O
authors.lastname: Nimi
authors.forename: Y
authors.initials: Y
authors.lastname: Murooka
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002789:Cholesterol Oxidase / Q000235:genetics*; D003062:Codon; D005796:Genes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
chemical_list: D003062:Codon; D015096:3-Hydroxysteroid Dehydrogenases; D002789:Cholesterol Oxidase