Formation of 8-hydroxydeoxyguanosine in DNA treated with fecapentaene-12 and -14.
Fecapentaene-12 and -14, direct-acting mutagens in human feces, were found to hydroxylate the C-8 position of guanine residues in DNA in vitro. Fecapentaene-12 or -14 was incubated with 0.5 mg of calf thymus DNA in 1 ml of reaction mixture at pH 7.4 for 2 h at 37 degrees C in the dark, and then 8-hydroxydeoxyguanosine (8-OH-dG) was analyzed. In these conditions 8-OH-dG was formed dose-dependently at levels of 1.1-4.6 residues/10(4) dG with concentrations of 0.5-3.0 mM of fecapentaene-12. Similar results were obtained with fecapentaene-14. The amount of 8-OH-dG in untreated DNA was 0.2-0.3 residue/10(4) dG.
Shioya
M
M
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Wakabayashi
K
K
Yamashita
K
K
Nagao
M
M
Sugimura
T
T
D000080242:8-Hydroxy-2'-Deoxyguanosine; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives*; D005609:Free Radicals; D066298:In Vitro Techniques; D011090:Polyenes / Q000633:toxicity*
D005609:Free Radicals; D011090:Polyenes; C037358:1-(1-glycero)tetradeca-1,3,5,7,9-pentaene; C037357:1-(1-glycero)dodeca-1,3,5,7,9-pentaene; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine