Differences between chick and turkey embryos in sensitivity to 3,3',4,4'-tetrachloro-biphenyl and in concentration/affinity of the hepatic receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin.
1. 3,3',4,4'-Tetrachlorobiphenyl (TCB) was 20-100 times more toxic in chick embryos than in turkey embryos when injected into eggs. 2. The ED50-value for induction of AHH activity by TCB in the liver of early chick and turkey embryos was estimated to be 0.6 and 6 micrograms/kg egg, respectively. 3. In both species alpha-naphthoflavone was more effective than metyrapone at inhibiting basal and TCB-induced AHH activities. 4. The TCDD receptor was detected in the liver of 7-day-old chick embryos, while it was not found in 9-day-old turkey embryo liver.
Brunström
B
B
Department of Zoophysiology, Uppsala University, Sweden.
Lund
J
J
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001571:Benzoflavones / Q000494:pharmacology; D002021:Buffers; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008797:Metyrapone / Q000494:pharmacology; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000187:drug effects*; D013045:Species Specificity; D014422:Turkeys / Q000196:embryology*
D001571:Benzoflavones; D002021:Buffers; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug; C011512:alpha-naphthoflavone; D011078:Polychlorinated Biphenyls; D001189:Aryl Hydrocarbon Hydroxylases; C028451:3,4,3',4'-tetrachlorobiphenyl; D008797:Metyrapone