A new immunomodulator, LF 1695--I. In vitro effects on the T lymphocyte lineage in man.
A new immunomodulator, LF 1695, was analyzed in vitro for its capacity to induce T cell markers (HTLA and OKT3, 4 and 8 antigens), to enhance the mitogen-induced lymphocyte proliferation, and to increase the Con A-induced suppressor activity. The inductive activity was compared with that of thymic hormone (thymosin fraction V). LF 1695 was capable of significantly augmenting the percentages of HTLA+ cells (10-15%) and of OKT 3+,T4+, or T8+ cells (13-28%) in human bone marrow prothymocytes. Maximum induction was observed at the concentration of 0.5 microgram/ml. In addition, LF 1695 significantly augmented the proliferation of human peripheral blood lymphocytes stimulated by mitogens (Concanavalin A, phytohemagglutinin, pokeweed mitogen and phorbol myristate acetate). LF 1695 also increased the Con A-induced suppressor activity of human lymphocytes. These data indicate that LF 1695 is active both on T cell precursors and on more mature T cells.
Othmane
O
O
Touraine
J L
JL
Sanhadji
K
K
Pascal
M
M
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000096:biosynthesis; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D010641:Phenotype; D010880:Piperidines / Q000494:pharmacology*; D013234:Stem Cells / Q000276:immunology; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000276:Adjuvants, Immunologic; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D010880:Piperidines; C046743:LF 1695