Evidence for the monomeric nature of thymosins.
According to gel-filtration experiments, alpha- and beta-thymosins appear to form oligomers, which are 4-5-fold larger than the corresponding polypeptides. However, on analysis by sedimentation equilibrium ultracentrifugation, prothymosin alpha and thymosin beta 4 showed relative molecular masses of 12,800 and 4600, which are close to the values calculated from their amino acid sequences, confirming their existence in solution as discrete monomeric entities.
Haritos
A A
AA
Zoological Laboratory, Faculty of Sciences, University of Athens, Greece.
Yialouris
P P
PP
Heimer
E P
EP
Felix
A M
AM
Hannappel
E
E
Rosemeyer
M A
MA
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D046911:Macromolecular Substances; D008970:Molecular Weight; D011498:Protein Precursors; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives / Q000302:isolation & purification; D013950:Thymus Gland / Q000032:analysis; D000099201:Thymosin beta(4)
D046911:Macromolecular Substances; D011498:Protein Precursors; D000077596:Thymalfasin; D013947:Thymosin; D000099201:Thymosin beta(4); C040782:prothymosin alpha; C044498:parathymosin alpha