Simultaneous existence of double minute chromosomes and a homogeneously staining region in a retinoblastoma cell line (Y79) and amplification of N-myc at HSR.
We observed double minute chromosomes (dmin) and a homogeneously staining region (HSR) in the same metaphase cells obtained from a retinoblastoma cell line, Y79. All of the 132 metaphases examined contained an HSR on the short arm of chromosome 1(1pHSR) and five cells (3.8%) had two to four dmin. To determine whether 1pHSR and dmin carried amplified N-myc sequences, we performed an in situ hybridization using an N-myc probe. Silver grains clustered on and along the 1pHSR, but not on the dmin. These findings indicate that the HSR on chromosome 1 is associated with amplification of N-myc in Y79 cells.
Inazawa
J
J
Department of Hygiene, Kyoto Prefectural University of Medicine, Japan.
Abe
T
T
Inoue
K
K
Nishigaki
H
H
Horiike
S
S
Taniwaki
M
M
Misawa
S
S
Takino
T
T
D002869:Chromosome Aberrations; D005134:Eye Neoplasms / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D007621:Karyotyping; D009857:Oncogenes; D012175:Retinoblastoma / Q000235:genetics*; D014407:Tumor Cells, Cultured