title: Antigen-triggered membrane potential changes in IgE-sensitized rat basophilic leukemia cells: evidence for a repolarizing response that is important in the stimulation of cellular degranulation.
abstract: We have studied Ag-induced membrane potential changes of rat basophilic leukemia cells by using the potential-sensitive dye, bis-(1,3-diethylthiobarbiturate)trimethineoxonol. A rapid membrane depolarization is triggered by a multivalent Ag, and it has a bell-shaped dose dependence that parallels the degranulation response but not the extent of cross-linking of the IgE-receptor complexes. As the temperature is reduced from 37 degrees C, this depolarization response slows and decreases in magnitude until complete inhibition is observed at 15 degrees C, similar to the temperature dependence previously observed for the Ag-stimulated rise in cytoplasmic Ca2+ and for degranulation. The results imply that a highly temperature-dependent step subsequent to Ag binding and cross-linking is necessary for the depolarization response. A partial return to the resting potential is seen to follow the depolarization response to Ag. This repolarization process is inhibited by quinidine.HCl and Ba2+ in parallel with an inhibition of the degranulation response. Repolarization is not affected by 4-aminopyridine or by the absence of K+ in the external buffer. These data suggest that the repolarization is caused by a previously uncharacterized K+ channel.
authors.affiliation: Department of Chemistry, Cornell University, Ithaca, NY 14853.
authors.forename: G F
authors.initials: GF
authors.lastname: Labrecque
authors.forename: D
authors.initials: D
authors.lastname: Holowka
authors.forename: B
authors.initials: B
authors.lastname: Baird
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001464:Barium; D001491:Basophils / Q000276:immunology / Q000502:physiology*; D002460:Cell Line; D003594:Cytoplasmic Granules / Q000276:immunology / Q000502:physiology*; D005456:Fluorescent Dyes; D007073:Immunoglobulin E / Q000502:physiology*; D007700:Kinetics; D007938:Leukemia / Q000276:immunology* / Q000503:physiopathology; D008564:Membrane Potentials; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D011802:Quinidine; D051381:Rats; D013696:Temperature; D013858:Thiobarbiturates
chemical_list: D000941:Antigens; D005456:Fluorescent Dyes; D015221:Potassium Channels; D013858:Thiobarbiturates; D001464:Barium; D007073:Immunoglobulin E; C025284:bis(1,3-diethylthiobarbiturate)trimethineoxonol; D011802:Quinidine