Isolation and characterization of two repetitive DNA fragments located near the centromere of the mouse X chromosome.
Two repetitive DNA fragments located on the mouse X chromosome are described. The fragments were isolated from a lambda phage library enriched in X-chromosomal sequences by flow sorting. Both fragments, which are repeated 20 to 50 times in the genome, were mapped to the mouse X chromosome by Southern blot hybridization to DNA from hybrid cells retaining the mouse X chromosome, by dosage analysis, and by in situ hybridization to mouse chromosomes. In mouse strain C57BL/10BK, one fragment appeared to be located only on the X chromosome, while the other fragment had homologous sequences on chromosome 11 in addition to the X chromosome. The latter fragment showed DNA variants between mouse strains, which are potentially useful for mapping. Both fragments cross-hybridized to another mouse species: Mus caroli. In this species, each fragment appeared to be located on the X chromosome, indicating that some X-chromosome repetitive sequences are partially conserved. In addition, one fragment cross-hybridized to human DNA.
Disteche
C M
CM
Tantravahi
U
U
Gandy
S
S
Eisenhard
M
M
Adler
D
D
Kunkel
L M
LM
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002503:Centromere; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D007621:Karyotyping; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D014960:X Chromosome
D004247:DNA