title: Activation of macrophages by Entamoeba histolytica extracts in mice.
abstract: The effect of Entamoeba histolytica extracts on the production of inflammatory macrophages and the release of hydrogen peroxide (H2O2) and superoxide (O2-) from these cells was examined in C57BL/6 mice. Two different strains of E. histolytica, either virulent (IP:0682:1) or nonvirulent (DKB), were used in this study. The number of macrophages recovered from the peritoneal cavity of mice treated 5 days previously with 150 micrograms of either strain of amoebic extracts was significantly higher than in the saline-treated groups. Macrophages from mice treated with 150 micrograms of the IP:0682:1 strain of amoebic extracts exhibited a powerful burst of oxidative metabolis when triggered with phorbol myristate acetate (PMA). Extract from the non-virulent strain was much less effective in activating macrophages for the production of oxidative metabolites. Thus, a crude extract preparation from E. histolytica, particularly the virulent strain, induces a strong macrophage inflammatory response in which the cells also produce high levels of H2O2 and O2-.
authors.affiliation: Montreal General Hospital Research Institute, Quebec, Canada.
authors.forename: E
authors.initials: E
authors.lastname: Ghadirian
authors.forename: P A
authors.initials: PA
authors.lastname: Kongshavn
mesh_terms: D000818:Animals; D004748:Entamoeba histolytica / Q000472:pathogenicity*; D006861:Hydrogen Peroxide / Q000096:biosynthesis; D007700:Kinetics; D008262:Macrophage Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D013481:Superoxides / Q000096:biosynthesis; D014774:Virulence
chemical_list: D013481:Superoxides; D006861:Hydrogen Peroxide