Biotin-labelled antigen screening test for toxoplasma IgM antibody.
A method for the simple preparation of biotin-labelled toxoplasma antigen was used with avidin peroxidase in an IgM-capture enzyme linked immunosorbent assay (BAM-ELISA). Although the overall predictive value of a positive result was only 38%, its low cost and 100% sensitivity makes it a very suitable screening test. Positive results can be confirmed by an alternative assay, thus providing a more economical and effective diagnostic service than either screening all sera by a commercial test or selecting sera for IgM testing.
Joss
A W
AW
Scottish Toxoplasma Reference Laboratory, Raigmore Hospital, Inverness, Scotland.
Skinner
L J
LJ
Moir
I L
IL
Chatterton
J M
JM
Williams
H
H
Ho-Yen
D O
DO
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D000953:Antigens, Protozoan / Q000276:immunology*; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D011237:Predictive Value of Tests; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007075:Immunoglobulin M; D001710:Biotin