Demonstration of immunoreactive sites on cartilage after in vivo administration of biotinylated anti-type II collagen antibodies.
Administration of biotinylated monoclonal antibodies provides the basis of a simple technique for identifying immunoreactive sites in vivo. Biotinylated anti-type II collagen antibodies were injected intraperitoneally into normal DBA/1 mice. The mice were sacrificed after 96 hr and the front paws removed and decalcified to allow tissue sectioning before snap-freezing. Binding of antibodies in vivo was visualized with affinity cytochemical staining using avidin-biotin-peroxidase complexes. Specific binding of antibodies to cartilaginous structures was seen after injection of 20-500 micrograms biotinylated monoclonal or polyclonal anti-type II collagen antibodies, but not after injection of a biotinylated control antibody. This technique should further the detection and localization studies of tissue components involved in the dynamics of physiological and pathological processes.
Jonsson
R
R
Department of Oral Pathology, University of Göteborg, Sweden.
Karlsson
A L
AL
Holmdahl
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D001710:Biotin; D002356:Cartilage / Q000378:metabolism*; D003094:Collagen / Q000276:immunology / Q000378:metabolism*; D005260:Female; D051379:Mice; D008811:Mice, Inbred DBA
D000911:Antibodies, Monoclonal; D001710:Biotin; D003094:Collagen