title: Evaluation of the new amniostat-FLM test for the detection of phosphatidylglycerol in contaminated fluids.
abstract: The Amniostat-FLM rapid slide test (Hana Biologics, Inc., Alameda, Calif.) for detection of phosphatidylglycerol has previously been evaluated and has shown good correlation with the more sophisticated two-dimensional thin-layer chromatographic method. A new, ultrasensitive kit has now been released with a lower level of detection of 0.5 microgram of phosphatidylglycerol per milliliter of fluid. This is the first report of this new kit, which we used with vaginal pool samples and with contaminated amniocentesis samples. We evaluated this kit for concordance with thin-layer chromatography results as well as fetal outcome. Of 48 vaginal pool samples, 41 (85%) showed concordance, whereas 39 of 42 (93%) contaminated amniocentesis samples were concordant, for an overall concordance of 89% (80 of 90 samples). Sixty-seven infants were delivered within 72 hours of the test and there were no cases of hyaline membrane disease in the presence of a positive test result. We conclude that this new, ultrasensitive kit is a good, time-saving, and reliable test for the detection of phosphatidylglycerol without the development of false-positive results even when tested on the worst possible fluid samples. A review of clinical studies involving the Amniostat-FLM is also presented.
authors.affiliation: Department of Obstetrics and Gynecology, Long Beach Memorial Womens Hospital, University of California, Irvine 90801-1428.
authors.forename: C V
authors.initials: CV
authors.lastname: Towers
authors.forename: T J
authors.initials: TJ
authors.lastname: Garite
mesh_terms: D000649:Amniocentesis; D000653:Amniotic Fluid / Q000032:analysis*; D001769:Blood; D002855:Chromatography, Thin Layer; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D006819:Hyaline Membrane Disease / Q000175:diagnosis; D007231:Infant, Newborn; D008470:Meconium; D010715:Phosphatidylglycerols / Q000032:analysis*; D011247:Pregnancy; D011933:Reagent Kits, Diagnostic; D012127:Respiratory Distress Syndrome, Newborn / Q000175:diagnosis
chemical_list: D010715:Phosphatidylglycerols; D011933:Reagent Kits, Diagnostic