The development of GABA-like immunoreactivity in the thoracic ganglia of the locust Schistocerca gregaria.
The development of GABA-like immunoreactivity was investigated in embryonic and juvenile locusts using an antibody raised against GABA-protein conjugates. GABA-like immunoreactivity was first detectable in the neuropile of embryonic ganglia at 55% development, and in neuronal somata at 62% development. The total number of immunoreactive somata increased between 62% and 85% embryonic development, and followed an anterio-posterior pattern of expression. At 85% development, the number of immunoreactive somata reached adult levels and no change in number was then seen. In embryonic stages and first and second juvenile instars two dorsal and four ventral groups of somata were labeled in all three thoracic ganglia, whilst in later juvenile instars one of the dorsal groups was visible as a separate entity only in the metathoracic ganglion. These early patterns were modified by alterations in the positions of some of the groups during late embryogenesis and during juvenile development to produce the adult pattern. The results show that the development of GABA expression is similar to that of other neurotransmitters. The characteristics of the development of immunoreactivity indicate that some of these immunoreactive clusters may be derived from clonally related neurones. Finally, we demonstrate the presence of immunoreactive somata and processes in embryos, which correspond to those of identified local and intersegmental interneurones studied in the adult.
O'Dell
D A
DA
Department of Zoology, University of Cambridge, United Kingdom.
Watkins
B L
BL
D000375:Aging / Q000276:immunology*; D000818:Animals; D006110:Grasshoppers / Q000378:metabolism*; D007150:Immunohistochemistry; D018377:Neurotransmitter Agents / Q000378:metabolism; D013900:Thoracic Nerves / Q000196:embryology / Q000276:immunology* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000276:immunology* / Q000378:metabolism
D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid