title: Enhancement of thyroid allograft survival following organ culture. Alteration of tissue immunogenicity.
abstract: Hyperbaric oxygen (95%, O2, 25 psi, 48-hr culture) resulted in prolonged thyroid allograft (B10.A) survival in both primary and sensitized recipients (B10.AQR). Recipients receiving noncultured thyroid allografts uniformly rejected the graft by 35 days, while 100% of cultured grafts survived. Noncultured thyroid grafts transplanted to skin-graft-primed recipients were rejected by 21 days. In contrast, 86% of cultured grafts transplanted to primed recipients were still functioning at 35 days. Donor spleen cells or peritoneal exudate cells transferred at the time of thyroid transplant were unable to stimulate cultured allograft rejection. Allografts histologically examined 35 days after transplant revealed, in some grafts, focal cellular infiltrates adjacent to normal, uninfiltrated tissue. To determine if tissue modification was the mechanism of prolonged allograft survival, hyperbaric-oxygen-cultured thyroids were examined for MHC class I expression. Immunoperoxidase staining with monoclonal antibody to MHC class I molecules showed that cultured thyroids were unstained in contrast to fresh thyroids that were uniformly stained. Cytotoxic T lymphocytes specific for H-2Kk administered 10 days following thyroid transplant were unable to eliminate cultured grafts (80% survival) but completely destroyed noncultured grafts. These results indicate that hyperbaric oxygen culture altered MHC class I expression such that it was no longer detectable by monoclonal antibody or cytotoxic T lymphocytes. Thus, the mechanism, explaining graft prolongation after hyperbaric culture in addition to passenger cell depletion, may be alteration of graft antigenicity such that the graft is no longer perceived as foreign.
authors.affiliation: Department of Surgery, University of Wisconsin Hospital, Madison 53792.
authors.forename: D A
authors.initials: DA
authors.lastname: Hullett
authors.forename: A S
authors.initials: AS
authors.lastname: Landry
authors.forename: D K
authors.initials: DK
authors.lastname: Leonard
authors.forename: H W
authors.initials: HW
authors.lastname: Sollinger
mesh_terms: D000818:Animals; D006085:Graft Survival; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006931:Hyperbaric Oxygenation; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D009926:Organ Preservation; D013961:Thyroid Gland / Q000276:immunology / Q000637:transplantation*
chemical_list: D015395:Histocompatibility Antigens Class I