The quantitative assay of the clustering activity of the lymphocytosis-promoting factor (pertussis toxin) of Bordetella pertussis on Chinese hamster ovary (CHO) cells.
An experimental design and a statistical method for the estimation of the clustering-response activity of lymphocytosis-promoting factor (LPF) in Chinese hamster ovary cells growing in wells on a microplate were investigated. The scoring method introduced by Ipsen was adopted to express the grade of the clustering response rather than the end-point method generally used. The scoring method was validated by statistical analyses. The grade of response varied with the location of the wells on a microplate, and thus the expression of the clustering activity of a test sample in terms of the end-point may be inadequate in terms of accuracy and reproducibility. It was shown that the allocation of test samples to individual wells according to a Latin square design minimized the effect of the location of wells on the clustering response. Under such experimental conditions, a fairly precise and reproducible method for the quantification of the clustering activity was developed.
Fujiwara
H
H
Department of General Biologics Control, National Institute of Health, Tokyo, Japan.
Iwasa
S
S
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D005260:Female; D010053:Ovary; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000032:analysis* / Q000633:toxicity
D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin