title: Characterization of vascular smooth muscle cell phenotype in long-term culture.
abstract: Studies of bovine carotid artery smooth muscle cells, during long-term in vitro subcultivation (up to 100 population doublings), have revealed phenotypic heterogeneity among cells, as characterized by differences in proliferative behavior, cell morphology, and contractile-cytoskeletal protein profiles. In vivo, smooth muscle cells were spindle-shaped and expressed desmin and alpha-smooth muscle actin (50% of total actin) as their predominant cytoskeletal and contractile proteins. Within 24 h of culture, vimentin rather than desmin was the predominant intermediate filament protein, with little change in alpha-actin content. Upon initial subcultivation, all cells were flattened and fibroblastic in appearance with a concomitant fivefold reduction in alpha-actin content, whereas the beta and gamma nonmuscle actins predominated. In three out of four cell lines studied, fluctuations in proliferative activity were observed during the life span of the culture. These spontaneous fluctuations in proliferation were accompanied by coordinated changes in morphology and contractile-cytoskeletal protein profiles. During periods of enhanced proliferation a significant proportion of cells reverted to their original spindle-shaped morphology with a simultaneous increase in alpha-actin content (20 to 30% of total actin). These results suggest that in long-term culture smooth muscle cells undergo spontaneous modulations in cell phenotype and may serve as a useful model for studying the regulation of intracellular protein expression.
authors.affiliation: University of Vermont, College of Medicine, Department of Physiology and Biophysics, Burlington 05405.
authors.forename: M
authors.initials: M
authors.lastname: Absher
authors.forename: J
authors.initials: J
authors.lastname: Woodcock-Mitchell
authors.forename: J
authors.initials: J
authors.lastname: Mitchell
authors.forename: L
authors.initials: L
authors.lastname: Baldor
authors.forename: R
authors.initials: R
authors.lastname: Low
authors.forename: D
authors.initials: D
authors.lastname: Warshaw
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000818:Animals; D002417:Cattle; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003893:Desmin / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D013997:Time Factors; D014746:Vimentin / Q000096:biosynthesis
chemical_list: D000199:Actins; D003893:Desmin; D014746:Vimentin