Cellular location of the cleavage event of the polymeric immunoglobulin receptor and fate of its anchoring domain in the rat hepatocyte.
Transcytosis of polymeric immunoglobulin (pIg) across glandular and mucosal epithelia is mediated by a member of the immunoglobulin supergene family, the pIg receptor. During transcellular routing, the receptor is cleaved and its ectoplasmic domain, known as secretory component (SC), is released into secretions bound to pIg. Using receptor-domain-specific antibodies, we have combined cell fractionation and immunoblotting of rat liver to examine the cellular routing of the receptor, the cellular location of the cleavage event and the fate of the anchor domain. Cleavage is a late event in receptor processing. It appears to occur at the canalicular plasma membrane, since intact receptor is present in this membrane domain and no SC is detected in whole liver homogenate or in cell fractions. The membrane anchor remaining after cleavage can be recovered in bile, as well as in a low-density fraction obtained after equilibrium centrifugation of liver (microsomal fractions) on sucrose density gradients. These data suggest that the membrane-anchor domain may be internalized as well as secreted together with SC into bile.
Solari
R
R
Swiss Institute for Experimental Cancer Research, University of Lausanne, Epalinges.
Schaerer
E
E
Tallichet
C
C
Braiterman
L T
LT
Hubbard
A L
AL
Kraehenbuhl
J P
JP
D000818:Animals; D001646:Bile / Q000032:analysis; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D007070:Immunoglobulin A / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000276:immunology*; D008297:Male; D011108:Polymers; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000032:analysis* / Q000378:metabolism; D012635:Secretory Component / Q000032:analysis
D007070:Immunoglobulin A; D011108:Polymers; D011971:Receptors, Immunologic; D012635:Secretory Component; C034639:polymeric IgA