Rapid detection of cutaneous herpes simplex virus infection with the polymerase chain reaction.
A simple and specific method for detecting herpes simplex virus infection in routinely processed paraffin-embedded biopsy specimens is described. DNA is extracted from paraffin blocks, and subjected to DNA amplification with the polymerase chain reaction. After 40 rounds, an amplified band can be detected after agarose gel electrophoresis and ethidium bromide staining. This band is specific for herpes simplex virus, because tissues infected with related viruses do not give this amplified band. We have been able to detect viral DNA in small punch skin biopsies with this procedure, which can take as little as 6 h.
Cao
M
M
Department of Pathology, University of California School of Medicine, San Francisco 94143-0506.
Xiao
X
X
Egbert
B
B
Darragh
T M
TM
Yen
T S
TS
D004279:DNA, Viral / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D004587:Electrophoresis, Agar Gel; D006561:Herpes Simplex / Q000175:diagnosis*; D006801:Humans; D008168:Lung / Q000382:microbiology; D012867:Skin / Q000382:microbiology
D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase