Inhibition of myeloid differentiation by inhibitors of ADP-ribosylation.
Inhibitors of ADP-ribosylation inhibited the myeloid differentiation of murine myelomonocytic leukemia, WEHI-3BD+ cells induced by granulocyte colony-stimulating factor. Benzamide, at 2.0 mM, inhibited 50% of the WEHI-3BD+ cell differentiation but had no significant effect on the proliferation. However, benzylaminododecylguanine hydrochloride and p-methoxylbenzylaminodecamethylene guanidine sulfate at 2.0 and 2.2 microM, respectively, inhibited 50% of proliferation but had no effect at all on differentiation. The differential effects of inhibitors provide a model to study the role of ADP-ribosylation in myeloid differentiation.
Wu
M C
MC
Department of Biochemistry, Texas College of Osteopathic Medicine, University of North Texas, Denton 76203.
Zaun
M R
MR
Wu
F M
FM
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D001853:Bone Marrow; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D007942:Leukemia, Experimental; D051379:Mice
D001549:Benzamides; D000246:Adenosine Diphosphate Ribose