title: On the structure of alpha-crystallin: construction of hybrid molecules and homopolymers.
abstract: The alpha A2 and alpha B2 subunits of bovine alpha-crystallin were purified by chromatofocussing in urea and assembled into homopolymers. Light-scattering measurements indicated their molecular masses were 360 and 420 kDa. The alpha A2 and alpha B2 polypeptides were also used to construct a series of hybrid molecules with alpha A/alpha B ratios ranging from 7:1 to 1:7. Sedimentation velocity analyses, isoelectric focussing under non-deaggregating conditions, circular dichroism spectroscopy and immunochemical analysis indicated that all of the subunits had copolymerized to alpha-crystallin-like aggregates with complete regeneration of the native structure. The polymers could be distinguished on the basis of their differing affinities for the antiserum. This was directly related to the proportion of alpha A2 subunits in each polymer. It was concluded that the alpha A2 and alpha B2 subunits are structurally equivalent and occupy equivalent site in the alpha-crystallin aggregates. It was also concluded that a micellar-like quaternary structure was consistent with most previous observations on the protein.
authors.affiliation: Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: J A
authors.initials: JA
authors.lastname: Thomson
authors.forename: R C
authors.initials: RC
authors.lastname: Augusteyn
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003459:Crystallins / Q000302:isolation & purification; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D008970:Molecular Weight; D011487:Protein Conformation; D055503:Protein Multimerization; D011863:Radioimmunoassay; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation
chemical_list: D003459:Crystallins; D046911:Macromolecular Substances