Specific binding of nuclear proteins to the promoter region of a maize nuclear rRNA gene unit.
The external spacer region of a nuclear rRNA gene unit of maize was analyzed for binding of nuclear proteins and revealed binding to the promoter region and to the 200-base pair repeat elements upstream of the promoter. The promoter binding site was further characterized by DNase I and exonuclease III foot-printing as well as by gel shift and South-Western experiments, revealing the binding of several proteins. Competition experiments indicate that the protein which interacts with the repeat units also binds to the promoter region. A model for the combined action of both binding regions in the regulation of transcription is presented.
Schmitz
M L
ML
Institut für Biologie III, Albert-Ludwigs-Universität, Freiburg, Federal Republic of Germany.
Maier
U G
UG
Brown
J W
JW
Feix
G
G
D001667:Binding, Competitive; D003850:Deoxyribonuclease I / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D011401:Promoter Regions, Genetic; D012335:RNA, Ribosomal / Q000032:analysis*; D012337:RNA, Ribosomal, 18S / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid
D009687:Nuclear Proteins; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D003850:Deoxyribonuclease I