Purification and characterization of two classes of neurotoxins from the funnel web spider, Agelenopsis aperta.
Two classes of paralytic toxins were isolated from the venom of Agelenopsis aperta and their chemical and larvicidal properties characterized. Five acylpolyamine toxins (alpha-agatoxins) of molecular masses 452, 488, 489, 504, and 505 Da produce immediate but reversible paralysis in Manduca sexta following injection. Six insecticidal peptides (mu-agatoxins) produce a gradual but irreversible paralysis. The complete amino acid sequences (36-38 residues) of the mu-agatoxins are presented. These peptides contain eight half-cystines and are quite similar in sequence. At least four of these toxins are amidated at the carboxyl terminus. The secondary structure of one of these toxins (mu-Aga V) was investigated.
Skinner
W S
WS
Zoecon Research Institute, Sandoz Crop Protection, Palo Alto, California 94304.
Adams
M E
ME
Quistad
G B
GB
Kataoka
H
H
Cesarin
B J
BJ
Enderlin
F E
FE
Schooley
D A
DA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001180:Arthropod Venoms / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009036:Moths; D009498:Neurotoxins; D011487:Protein Conformation; D013111:Spider Venoms / Q000145:classification / Q000302:isolation & purification* / Q000633:toxicity
D000596:Amino Acids; D001180:Arthropod Venoms; D009498:Neurotoxins; D013111:Spider Venoms