title: The effects of prostaglandin E1 on lung injury complicating hyperdynamic sepsis in sheep.
abstract: We examined the hypothesis that PGE1 would reduce the severity of lung injury in sheep rendered septic by cecal ligation and perforation (CLP). Twenty-four to 30 h after CLP, septic lung injury was documented in 37 sheep because pulmonary lymph flow (Qlym) was increased above the baseline, nonseptic study (delta = +7.38 +/- 5.1 ml/h; p less than 0.05), whereas the lymph-to-plasma total protein ratios remained unchanged. During a subsequent 24-h "septic treatment" study period, Qlym continued to increase in an untreated study group (septic treatment minus septic delta = +10.24 +/- 4.9 ml/h; p less than 0.05), but not in sheep treated with PGE1 by continuous infusion at two doses, 1 micrograms/kg/h ("low-dose": delta Qlym = -0.04 +/- 6.1 ml/h; p = NS) and 1 microgram/kg/min ("high-dose": delta Qlym = -0.04 +/- 6.1 ml/h; p = NS. Mean pulmonary artery pressures (Ppa) increased in the untreated group during the septic treatment period (delta = +3.74 +/- 4.8 mm Hg; p less than 0.01), but not during PGE1 infusion in either of the low-dose (delta Ppa = -4.1 +/- 5.7 mm Hg; p less than 0.04) or the high-dose (delta Ppa = -0.1 +/- 6.2 mm Hg; P = NS) groups. Unlike other study groups, the PaO2 fell in the high-dose PGE1 group during the septic treatment study (delta PaO2 = -15.0 +/- 9.6 mm Hg; p less than 0.01). During a study period of drug withdrawal 24 h after the septic-treatment period, Qlym again increased in the low-dose PGE1 group such that the untreated and PGE1 groups were no longer dissimilar.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Richard Ivey Critical Care Trauma Centre, Victoria Hospital, London, Ontario, Canada.
authors.forename: W J
authors.initials: WJ
authors.lastname: Sibbald
authors.forename: D
authors.initials: D
authors.lastname: Campbell
authors.forename: R R
authors.initials: RR
authors.lastname: Raper
authors.forename: F S
authors.initials: FS
authors.lastname: Rutledge
authors.forename: H
authors.initials: H
authors.lastname: Cheung
mesh_terms: D000527:Alprostadil / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D001424:Bacterial Infections / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D001794:Blood Pressure / Q000187:drug effects; D002302:Cardiac Output; D006439:Hemodynamics / Q000187:drug effects; D007958:Leukocyte Count; D008171:Lung Diseases / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D008196:Lymph / Q000502:physiology; D009504:Neutrophils / Q000473:pathology; D011651:Pulmonary Artery / Q000503:physiopathology; D012756:Sheep
chemical_list: D000527:Alprostadil