title: [W-mutagenesis in the bisulfite-treated lambda phage].
abstract: Survival of phage lambda cI857 inactivated by bisulfite (pH 5.6, 37 degrees C) is higher (the dose modification factor approx. 1.2) and frequency of bisulfite-induced c-mutations 2-4-fold lower on the lawn of the wild-type strain ung+, as compared to ung-1 mutant deficient in uracil-DNA glycosylase. Irradiation of host cells by a moderate UV dose inducing SOS repair system enhances the frequency of bisulfite-induced c-mutations 2-3-fold in the wild-type (ung+) host, but not in the ung-1 mutant. It is suggested that W-mutagenesis in bisulfite-treated lambda phage in the ung+ cells is due to SOS repair of apyrimidinic sites which are produced during excision of uracil residues, the products of cytosine deamination.
authors.forename: L V
authors.initials: LV
authors.lastname: Konevega
authors.forename: V L
authors.initials: VL
authors.lastname: Kalinin
mesh_terms: D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D045647:DNA Glycosylases; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004279:DNA, Viral / Q000235:genetics; D009154:Mutation; D009699:N-Glycosyl Hydrolases / Q000235:genetics; D013447:Sulfites / Q000494:pharmacology*; D014466:Ultraviolet Rays; D051981:Uracil-DNA Glycosidase
chemical_list: D004279:DNA, Viral; D013447:Sulfites; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; C042345:hydrogen sulfite
vernacular_title: W-mutagenez u faga liambda, obrabotannogo bisul'fitom.