[Spectrophotometric method for determining the stability of lysosomes from animal tissues depending on the hydrogen ion concentration].
Rapid spectrophotometric method of lysosome stability determination depending on hydrogen ion concentration is described. The time of analysis is decreased by 5-6 h in comparison with enzymic method. The process of lysosome degradation was linear at pH 6. The incubation mixture acidity dependence curve of lysosome lysis extend was complex. The lysosome lysis rate rapidly increased at pH much less than 6 less than pH. Lysosome incubation at 0-4 degrees C during 24 h decreased its sensitivity to incubation mixture acidity within the whole investigated pH range. Isolated lysosome acid resistance may be used as an index of its stability and lability in vivo and in vitro by various physicochemical factors. Percentage of initial absorbtion (A520) and initial lysosome lysis rate (delta A520/min) may be index of such effect.
Siatkin
S P
SP
Frolov
V A
VA
D000042:Absorption; D000818:Animals; D002458:Cell Fractionation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000032:analysis; D008247:Lysosomes / Q000032:analysis*; D008297:Male; D051381:Rats; D013053:Spectrophotometry / Q000379:methods*; D013535:Suspensions; D013997:Time Factors
D013535:Suspensions
Spektrofotometricheskiĭ metod opredeleniia stabil'nosti lizosom iz tkaneĭ zhivotnykh v zavisimosti ot kontsentratsii ionov vodoroda.