Identification of 3H-bradykinin binding sites in PC-12 cells and brain.
3H-Bradykinin binding sites with the characteristics of receptors were identified in homogenates of bovine hippocampus and PC-12 cells. The characteristics of the binding were similar in both types of tissue and paralleled those of the B2 bradykinin receptor. Following exposure to nerve growth factor for 72 hours, the number of BK binding sites in PC-12 cells significantly increased, but other characteristics of the binding remained unchanged.
Kozlowski
M R
MR
Department of Screening and Biochemical Research, Bristol-Myers Company, Wallingford, CT 06492-7660.
Rosser
M P
MP
Hall
E
E
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001920:Bradykinin / Q000378:metabolism*; D002417:Cattle; D006624:Hippocampus / Q000378:metabolism*; D010673:Pheochromocytoma / Q000378:metabolism*; D051381:Rats; D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter / Q000378:metabolism / Q000502:physiology*; D014316:Tritium; D014407:Tumor Cells, Cultured
D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter; D014316:Tritium; D001920:Bradykinin