A new locus (NIA 1) in Arabidopsis thaliana encoding nitrate reductase.
We have isolated two nitrate reductase genes and their corresponding cDNAs from Arabidopsis thaliana. Sequences of the two cDNAs, when compared to a sequence of a barley cDNA clone, confirm their identity as nitrate reductase clones and show that they are closely related. The two genes have been mapped using restriction fragment length polymorphisms; gNR2 is close to the previously identified chl-3 locus and is probably identical to it, while gNR1 maps to a new locus (NIA1) on chromosome 1, near gl-2.
Cheng
C L
CL
Department of Genetics, Harvard Medical School, Boston, MA.
Dewdney
J
J
Nam
H G
HG
den Boer
B G
BG
Goodman
H M
HM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011233:Precipitin Tests; D015183:Restriction Mapping
D004247:DNA; D009565:Nitrate Reductases; D050901:Nitrate Reductase