Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line.
A novel basic heparin-binding monocyte chemotactic factor (MCF) was purified to homogeneity from the conditioned media of human myelomonocytic cell line THP-1 based on its in vitro monocyte chemotactic activity. The purified MCF was homogenous and estimated to be 15 kD on SDS-PAGE. Purified MCF stimulated normal human monocytes to be growth inhibitory in vitro at 2-3 d for several human tumor cell lines. This represents the first report of the identification and purification of a chemoattractant cytokine that also activates monocytes but is distinct from interferons and other known cytokines.
Matsushima
K
K
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701.
Larsen
C G
CG
DuBois
G C
GC
Oppenheim
J J
JJ
D002460:Cell Line; D002630:Chemotactic Factors / Q000302:isolation & purification*; D002634:Chemotaxis, Leukocyte; D002851:Chromatography, High Pressure Liquid; D006493:Heparin / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation; D008970:Molecular Weight; D009000:Monocytes / Q000032:analysis*
D002630:Chemotactic Factors; D006493:Heparin