title: The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase.
abstract: Recently we reported the sequence of a cDNA clone (pUOX-1), isolated from a lambda gt11 cDNA library, which encoded for rat liver urate oxidase (EC 1.7.3.3), but this clone lacked the nucleotide sequences encoding the N-terminal region for this enzyme. Using the cDNA insert from the pUOX-1 clone as a probe, we have now isolated a full length cDNA clone, pUOX-2, from a lambda gt10 library by plaque hybridization. Nucleotide sequence analysis of the pUOX-2 clone showed that it has 1379 base pairs with an open reading frame coding for 303 amino acid residues corresponding to a molecular mass of 34,931 daltons. In addition to the open reading frame the pUOX-2 contains 439 bp of 3'-untranslated and 41 bp of 5'-untranslated sequences. The consensus polyadenylation signal AATAAA precedes a stretch of poly(A)+ residues at the 3' end.
authors.affiliation: Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611.
authors.forename: K
authors.initials: K
authors.lastname: Alvares
authors.forename: M R
authors.initials: MR
authors.lastname: Nemali
authors.forename: P G
authors.initials: PG
authors.lastname: Reddy
authors.forename: X D
authors.initials: XD
authors.lastname: Wang
authors.forename: M S
authors.initials: MS
authors.lastname: Rao
authors.forename: J K
authors.initials: JK
authors.lastname: Reddy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D051381:Rats; D014503:Urate Oxidase / Q000235:genetics*
chemical_list: D003062:Codon; D004247:DNA; D014503:Urate Oxidase