title: The high molecular weight multicatalytic proteinase, macropain, exists in a latent form in human erythrocytes.
abstract: The high molecular weight multicatalytic proteinase, macropain, has been purified from human erythrocytes in two forms that differ in caseinolytic activity up to 100-fold. Each form has a native molecular weight of 600,000 and is composed of a number of subunits ranging in molecular weights from 35,000 to 21,000. Although the two proteinase forms share a number of electrophoretically indistinguishable subunits, there are also subunits unique to the respective forms. The less active proteinase represents a latent enzyme because it was fully activated by two procedures including dialysis against water and pretreatment with low concentrations of sodium dodecyl sulfate. These procedures caused differential changes in the caseinolytic and two peptidase activities of the proteinase. An Mr 35,000 subunit, characteristic of latent macropain, is immunologically related to at least one of the other components of active macropain and disappeared after proteinase activation by dialysis. Nevertheless, loss of this subunit was not the cause of the increased activity. These results suggest that the proteolytic activity of cells may be regulated by the activation of the latent form of macropain.
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: M J
authors.initials: MJ
authors.lastname: McGuire
authors.forename: M L
authors.initials: ML
authors.lastname: McCullough
authors.forename: D E
authors.initials: DE
authors.lastname: Croall
authors.forename: G N
authors.initials: GN
authors.lastname: DeMartino
mesh_terms: D002364:Caseins / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000097:blood* / Q000378:metabolism*; D003956:Dialysis; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D002364:Caseins; D003374:Coumarins; D009097:Multienzyme Complexes; D009842:Oligopeptides; C059197:succinyl-leucyl-leucyl-valyl-tyrosyl-4-trifluoromethylcoumarin-7-amide; D012967:Sodium Dodecyl Sulfate; C038043:valyl-leucyl-arginine-4-methoxy-2-naphthylamide; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex