A simple method for measuring immunoglobulin synthesis in vivo in the spleen of chicks (Gallus domesticus).
1. Spleen immunoglobulin (IgG) synthesis was measured in vivo in chicks at 3 weeks of age by a large dose injection of labelled phenylalanine in combination with the isolation of IgG by immunological precipitation against anti-chicken IgG. 2. No appreciable amount of radioactivity was detected in serum IgG for the first 60 min of the isotope injection via the wing vein, indicating a minimum time lag necessary for the secretion of newly synthesized IgG into the circulation. 3. Synthesis rates of total spleen protein and spleen IgG were found to be 17.5 and 4.8 mg/day, respectively, suggesting that IgG synthesis would contribute to 27% of the total protein synthesis in the spleen of young chicks.
Muramatsu
T
T
Laboratory of Animal Nutrition, School of Agriculture, Nagoya University, Japan.
Kuzuyama
S
S
Aoyagi
Y
Y
Okumura
J
J
D000818:Animals; D002645:Chickens / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D014176:Protein Biosynthesis; D013154:Spleen / Q000276:immunology* / Q000378:metabolism
D007074:Immunoglobulin G