Detection of Thy-1+ cells in the developing thymus of the lizard, Chalcides ocellatus.
The ontogenic appearance of Thy-1+ cells was studied in the thymus of the lizard, Chalcides ocellatus, by indirect immunofluorescence using a rabbit antibrain antiserum produced against surface determinants shared by both the thymus and the brain lizards. Thymic Thy-1+ cells were first detected at stage 37. They increased rapidly reaching adult numbers by stage 41. Apparently Thy-1+ cells differentiate from lymphoid precursors arriving at the thymus analage before stage 37. The results are compared to those found during the differentiation of Thy-1+ thymocyte in murine thymus and agree with former reports on the thymic development in Chalcides using rabbit antisera against adult thymocyte marker(s).
el Deeb
S
S
Zoology Department, Faculty of Science, Cairo University, Egypt.
Saad
A H
AH
Zapata
A
A
D000818:Animals; D000831:Animals, Newborn; D000954:Antigens, Surface / Q000032:analysis*; D001921:Brain / Q000276:immunology; D003429:Cross Reactions; D005260:Female; D005455:Fluorescent Antibody Technique; D008116:Lizards / Q000196:embryology / Q000254:growth & development / Q000276:immunology*; D011247:Pregnancy; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000196:embryology / Q000254:growth & development / Q000276:immunology*
D000954:Antigens, Surface; D018800:Thy-1 Antigens