Increased dolichol content in glucocorticoid-sensitive human T-cell leukemia line grown in the presence of dexamethasone.
Two cell lines, derived from human T-cell acute leukemia, one glucocorticoid sensitive (CEM-C7) and the other glucocorticoid resistant (CEM-C1) were grown in the presence of 1 x 10-7 M dexamethasone and were analyzed for their dolichol content. After 24 hrs of incubation, dolichols became significantly elevated in the sensitive but not in the resistant line. Lovastatin, the specific inhibitor of cholesterol synthesis did not affect dolichol levels in either of the two cell lines. The results raise the possibility that dolichol accumulation might be involved in the early stages of the glucocorticoid-induced apoptosis (directed cell killing).
Dutta
A
A
Department of Microbiology, University of Kansas Medical Center, Kansas City, KS 66103
Parikh
B
B
Maben
R
R
Melnykovych
G
G
D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000494:pharmacology*; D004286:Dolichols / Q000096:biosynthesis*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000378:metabolism*; D008148:Lovastatin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004286:Dolichols; D003907:Dexamethasone; D008148:Lovastatin