title: In vitro degradation of opioid peptides by human placental aminopeptidase M.
abstract: The degradation of opioid peptides by human placental aminopeptidase M was studied by measuring liberated amino acids by high performance liquid chromatography. The purified placental aminopeptidase M actively degraded Met-enkephalin (Met-Enk) and Leu-enkephalin (Leu-Enk) completely into constituent 5 amino acids. The activities as a function of tyrosine liberation were observed for Met-Enk followed by Leu-Enk, beta-neoendorphin, dynorphin, and beta-endorphin.
authors.affiliation: Department of Obstetrics/Gynaecology, Nagova University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Furuhashi
authors.forename: S
authors.initials: S
authors.lastname: Mizutani
authors.forename: O
authors.initials: O
authors.lastname: Kurauchi
authors.forename: M
authors.initials: M
authors.lastname: Kasugai
authors.forename: O
authors.initials: O
authors.lastname: Narita
authors.forename: Y
authors.initials: Y
authors.lastname: Tomoda
mesh_terms: D000626:Aminopeptidases / Q000494:pharmacology*; D018826:CD13 Antigens; D004723:Endorphins / Q000378:metabolism*; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
chemical_list: D004723:Endorphins; D000626:Aminopeptidases; D018826:CD13 Antigens