title: [Determination of serum and plasma erythropoietin in mice with phenylhydrazine-induced anemia].
abstract: The simple, specific and sensitive erythropoietin bioassay in serum and plasma from phenylhydrazine treated mice is described, based on H3-thymidine incorporation into divided hemopoietic cells. Spleen cells taken from mice on the third day following the second of 2 daily injections of phenylhydrazine were cultured in 24 hours in the presence of test material. Following incubation for 2 hours with H3-thymidine solution radioactivity was measured.
authors.forename: T E
authors.initials: TE
authors.lastname: Manakova
authors.forename: N A
authors.initials: NA
authors.lastname: Setkov
mesh_terms: D000740:Anemia / Q000097:blood* / Q000139:chemically induced; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002478:Cells, Cultured; D004921:Erythropoietin / Q000097:blood*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010659:Phenylhydrazines; D013154:Spleen / Q000166:cytology
chemical_list: D010659:Phenylhydrazines; C030299:phenylhydrazine; D004921:Erythropoietin
vernacular_title: Opredelenie aktivnosti éritropoétina v syvorotke i plazme mysheĭ pri anemii, vyzvannoĭ fenilgidrazinom.