title: Selective mutagenic activation by cytochrome P3-450 of carcinogenic arylamines found in foods.
abstract: Heterocyclic arylamines found in cooked foods including fish and beef are potent mutagens and carcinogens. The purpose of this investigation was to determine the specificity of cytochromes P1-450 and P3-450 toward the metabolic activation of these arylamines. We used a novel mutagenicity test system which combined human cells expressing either recombinant cytochrome P1-450 or P3-450 with Salmonella typhimurium to score mutants. Cytochrome P3-450, a single isoform of the cytochrome P-450 supergene family, bioactivated these food mutagens. Cytochrome P1-450 showed little or no activation of these arylamines but was the isoform predominantly responsible for the activation of the aromatic hydrocarbon benzo[a]pyrene-7,8-diol. This assay system should serve to define the specificities of individual cytochromes P-450 in the metabolic activation of carcinogens.
authors.affiliation: Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, MD 20892.
authors.forename: E G
authors.initials: EG
authors.lastname: Snyderwine
authors.forename: N
authors.initials: N
authors.lastname: Battula
mesh_terms: D000588:Amines / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005504:Food Analysis; D006367:HeLa Cells; D006801:Humans; D007093:Imidazoles / Q000378:metabolism*; D066298:In Vitro Techniques; D009152:Mutagenicity Tests
chemical_list: D000588:Amines; D000911:Antibodies, Monoclonal; D007093:Imidazoles; D003577:Cytochrome P-450 Enzyme System