Characterization of kappa opioid receptors in the rabbit ear artery.
Quantitative characterization of the kappa opioid receptor in the rabbit ear artery was carried out using three kappa-selective agonist compounds, dynorphin-(1-13), U-69593 and ethylketocyclazocine. Kinetic analysis was performed using the antagonist, MR 2266. Two other in vitro preparations were studied for comparison: the mouse was deferens and rabbit was deferens. To avoid mu receptor action in the mouse was deferens the irreversible mu receptor antagonist, beta-funaltrexamine, was used. It was demonstrated that, using the highly selective kappa agonist compound U-69593, Ke values for MR 2266 obtained in the three assay systems were not significantly different. These results suggest that kappa receptors present in these three tissues share identical properties.
Berzetei
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IP
Department of Pharmacology, College of Medicine, University of California, Irvine 92717.
Fong
A
A
Yamamura
H I
HI
Duckles
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SP
D000818:Animals; D044482:Benzeneacetamides; D001575:Benzomorphans / Q000494:pharmacology; D003496:Cyclazocine / Q000031:analogs & derivatives / Q000494:pharmacology; D004399:Dynorphins / Q000494:pharmacology; D016314:Ethylketocyclazocine; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009292:Narcotic Antagonists / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology; D011817:Rabbits; D011957:Receptors, Opioid / Q000378:metabolism*; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu
D044482:Benzeneacetamides; D001575:Benzomorphans; D009292:Narcotic Antagonists; D010446:Peptide Fragments; D011759:Pyrrolidines; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; C027247:MR 2266; D016314:Ethylketocyclazocine; C026843:dynorphin (1-13); D004399:Dynorphins; C045444:U 69593; D003496:Cyclazocine