Molecular cloning and nucleotide sequence of human pancreatic prechymotrypsinogen cDNA.
The cDNA clone encoding human prechymotrypsinogen was isolated from a human pancreas cDNA library and its nucleotide sequence was determined. The sequence consists of a 16 bp 5' non-coding region, a 789 bp amino acid coding region and a 60 bp 3' non-coding region. The predicted product consists of 263 amino acids, including 18 amino acids for a signal peptide and 15 amino acids possible for an activation peptide. Southern blot analyses using the cloned cDNA as a probe revealed that human genomic DNA carries at least two genes that are related to chymotrypsinogen.
Tomita
N
N
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Izumoto
Y
Y
Horii
A
A
Doi
S
S
Yokouchi
H
H
Ogawa
M
M
Mori
T
T
Matsubara
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002919:Chymotrypsinogen / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004247:DNA; D002919:Chymotrypsinogen