Measles virus editing provides an additional cysteine-rich protein.
The measles virus (MV) phosphoprotein (P) gene encodes two known proteins, P (Mr approximately 70,000), involved in viral transcription, and, in a different reading frame, C (Mr approximately 20,000). By a combination of cDNA cloning, cDNA and RNA sequencing, and in vitro translation, we demonstrate here that the MV P gene also expresses a third product (Mr approximately 46,000) containing the amino-terminal region of P but a different, cysteine-rich carboxy-terminal motif. This third protein is translated from mRNAs in which one G residue has been inserted after three genomically encoded Gs, a modification found in about 50% of the P mRNAs. A smaller fraction of transcripts contain several additional G residues.
Cattaneo
R
R
Institut für Molekularbiologie I, Universität Zürich, Switzerland.
Kaelin
K
K
Baczko
K
K
Billeter
M A
MA
D003545:Cysteine; D005786:Gene Expression Regulation; D005814:Genes, Viral; D008459:Measles virus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D012367:RNA, Viral; D014764:Viral Proteins; D003545:Cysteine