Immunoglobulin heavy chain switch region polymorphisms are not associated with type 1 diabetes.
In order to ascertain whether the immunoglobulin heavy chain genes are important in the aetiology of Type 1 diabetes, we have used restriction fragment length polymorphism (RFLP) analysis of genomic DNA to study 148 Caucasoid subjects with Type 1 diabetes and 146 normal Caucasoid subjects. A DNA probe homologous to the switch regions for the IgM (S mu) and IgA1 (S alpha 1) genes when used in conjunction with the restriction endonuclease Sst I detects RFLPs at both these loci. There were no significant differences in phenotype or gene frequencies for the alleles of S mu or S alpha 1 in the patients when compared with control subjects; nor were there significant associations of S mu or S alpha 1 with HLA-DR type or gender.
Millward
B A
BA
Department of Molecular Immunogenetics, Guy's Hospital, London, UK.
Hitman
G A
GA
Cassell
P G
PG
Sachs
J A
JA
Welsh
K I
KI
Demaine
A G
AG
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000276:immunology*; D005803:Genes, Immunoglobulin; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007134:Immunoglobulin Switch Region / Q000235:genetics*; D007223:Infant; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012016:Reference Values
D006680:HLA Antigens; D007143:Immunoglobulin Heavy Chains