Development of a solid phase RIA for human plasma somatostatin.
Somatostatin is widely distributed in tissues of biological fluids. In the present study we tested the validity of a new radioimmunoassay by a solid phase immunoextraction. Sensitivity was 1.8 pg/mL and the within-assay precision with two different pools of human plasma was 12.2% (CV) at a concentration of 19.2 +/- 2.34 pg/mL (mean +/- SD) and 11.9% at 8.53 +/- 1.02 pg/mL. The between assay precision was 14.2% (CV) at a concentration of 11.8 +/- 1.68 pg/mL. The accuracy was good as tested by the dilution test (slope: 0.956, Y-intercept 3.07 pg/mL, r = 0.997) and the recovery test (slope: 1.008, Y-intercept 2.22 pg/mL, r = 0.999). The method resulted in good correlation with a current extraction method (no. 65, y = 1.45 + 0.975 x, r = 0.862, P less than 0.001). Basal value of somatostatin in the plasma of 32 normal subjects was 11.5 +/- 5.80 pg/mL (mean +/- SD).
Brocchi
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A
Dipartimento di Fisiopatologia Clinica, Unità di Medicina Nucleare, Firenze, Italy.
Tommasi
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M
D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D012016:Reference Values; D013004:Somatostatin / Q000097:blood*
D013004:Somatostatin