A radiochemical test for phospholipase A2 catalytic activity.
A position-specifically labelled phosphatidylcholine is the substrate for the selective determination of Phospholipase A2 in serum, ascites and tissue samples. Optimal reaction conditions and simplifications of handling are discussed. A control group of human serum samples ranged up to 2.1 U/l. The maximum serum activity in samples of patients with acute pancreatitis was 126 U/l. In human ascites activities up to 380 U/l were measured. The method described here turned out to be a practicable instrument for the determination of phospholipase A2 activity using only commercially available reagents.
Schädlich
H R
HR
Abteilung für Allgemeine Chirurgie, Universität Ulm.
Büchler
M
M
Beger
H G
HG
D000208:Acute Disease; D001201:Ascites / Q000201:enzymology*; D006801:Humans; D008103:Liver Cirrhosis / Q000201:enzymology; D010195:Pancreatitis / Q000201:enzymology; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2; D011854:Radiochemistry
D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2