A comparison of polyclonal antisera to pregnanediol 3 alpha glucuronide obtained from three different rabbits and their use in ELISA.
The variation in the estimates of urinary pregnanediol glucuronide obtained by ELISA with 12 polyclonal antisera has been assessed. Of the 12 antisera, eight gave values comparable with each other and could be used interchangeably in the ELISA. Thus a large stock of polyclonal antibody which gave consistent values was generated and can now be used in diagnostic ELISA over a long period of time. This procedure is more economical than the development of monoclonal antibodies for the same purpose.
Sankolli
G M
GM
Institute for Research in Reproduction, Bombay, India.
Joshi
U M
UM
D000818:Animals; D000906:Antibodies / Q000592:standards*; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D011276:Pregnanediol / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D011817:Rabbits
D000906:Antibodies; C022270:pregnanediol-3 alpha-glucuronide; D011276:Pregnanediol