Studies of mutagenesis and neoplastic transformation by bivalent metal ions and ionizing radiation.
We examined the influence of nontoxic concentrations of each of two essential (Zn++ and Mn++) and one nonessential (Ni++) bivalent metal ions on spontaneous and radiation-induced neoplastic transformation and specific gene mutations in mammalian cells. All three metals induced low levels of transformation in mouse BALB/3T3 cells but exerted no mutagenic effect in CHO cells (hprt locus) over a broad range of concentrations. Continuous incubation for 8 or 15 days with each of the metal ions did not enhance the frequency of cell killing, transformation, or mutations induced by acute exposure to x-rays. Zn++, however, had a small but consistent protective effect on the induction of all three endpoints by x-irradiation.
Little
J B
JB
Department of Cancer Biology, Harvard University School of Public Health, Boston, Massachusetts 02115.
Frenial
J M
JM
Coppey
J
J
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000528:radiation effects*; D002478:Cells, Cultured; D008274:Magnesium / Q000494:pharmacology*; D051379:Mice; D009154:Mutation; D009532:Nickel / Q000494:pharmacology*; D013997:Time Factors; D015032:Zinc / Q000494:pharmacology*
D009532:Nickel; D008274:Magnesium; D015032:Zinc