Failure of zinc to prevent dysmorphogenesis of cultured rat conceptuses by anti-yolk sac antiserum.
Day 10 rat conceptuses were cultured for 48h in the presence of either cadmium or anti-visceral yolk sac antiserum (AVYS). Cadmium was embryotoxic at concentrations exceeding 0.25 micrograms/ml whilst AVYS caused embryonic dysmorphogenesis, particularly affecting the optic vesicles, at concentrations of 2 microliters/ml and above. The effect of pretreatment with zinc on embryotoxicity caused by cadmium or AVYS was studied. Zinc ameliorated the effects of cadmium but had no effect on AVYS-induced embryonic abnormalities. In a second set of experiments inhibition of 125I-labelled PVP uptake by the yolk sac of cultured whole conceptuses was studied. Cadmium and AVYS both inhibited uptake compared to control cultures. Zinc again ameliorated the effect of cadmium but had no action against AVYS-induced inhibition. These results are in contrast to our previous findings using isolated cultured yolk sacs in which zinc ameliorated the inhibitory effects on 125I-labelled PVP uptake of both cadmium and AVYS. These data show that in experiments using the isolated cultured yolk sac and the intact cultured conceptus, a qualitatively different response in yolk sac behaviour is observed under similar experimental conditions.
Marlow
R
R
Department of Toxicology, Smith Kline & French Research Limited, Hertfordshire, U.K.
Freeman
S J
SJ
D000818:Animals; D002104:Cadmium / Q000037:antagonists & inhibitors / Q000633:toxicity; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques; D007106:Immune Sera / Q000494:pharmacology; D009024:Morphogenesis / Q000187:drug effects*; D010873:Pinocytosis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D015017:Yolk Sac / Q000276:immunology / Q000502:physiology*; D015032:Zinc / Q000494:pharmacology*
D003470:Culture Media; D007106:Immune Sera; D002104:Cadmium; D015032:Zinc