Degradation of oxidatively denatured proteins in Escherichia coli.
When exposed to oxidative stress, by oxygen radicals or H2O2, E. coli exhibited decreased growth, decreased protein synthesis, and dose-dependent increases in protein degradation. The quinone menadione induced proteolysis when cells were incubated in air, but was not effective when cells were incubated without oxygen. Anaerobically grown cells also exhibited significantly lower proteolytic capacity than did cells that were grown aerobically. Xanthine plus xanthine oxidase (which generate O2- and H2O2) caused a stimulation of proteolysis which was inhibitable by catalase, but not by superoxide dismutase: Indicating that H2O2 was responsible for the increased protein degradation. Indeed, H2O2 alone was effective in inducing increased intracellular proteolysis. Two-dimensional polyacrylamide gel electrophoresis of [3H]leucine labeled E. coli revealed greater than 50% decreases in the concentrations of 10-15 cell proteins following H2O2 or menadione exposure, while several other proteins were less severely affected. To test for the presence of soluble proteases, we prepared cell-free extracts of E. coli and incubated them with radio-labeled protein substrates. E. coli extracts degraded casein and globin polypeptides at rapid rates but showed little activity with native proteins such as superoxide dismutase, hemoglobin, bovine serum albumin, or catalase. When these same proteins were denatured by exposure to oxygen radicals or H2O2, however, they became excellent substrates for degradation in E. coli extracts. Studies with albumin revealed correlations greater than 0.95 between the degree of oxidative denaturation and proteolytic susceptibility. Pretreatment of E. coli with menadione or H2O2 did not increase the proteolytic capacity of cell extracts; indicating that neither protease activation, nor protease induction were required.(ABSTRACT TRUNCATED AT 250 WORDS)
Davies
K J
KJ
Department of Biochemistry, University of Southern California, Los Angeles 90033.
Lin
S W
SW
D001426:Bacterial Proteins / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004789:Enzyme Activation; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005609:Free Radicals; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000378:metabolism*; D011489:Protein Denaturation; D013482:Superoxide Dismutase / Q000378:metabolism; D014812:Vitamin K / Q000494:pharmacology
D001426:Bacterial Proteins; D005609:Free Radicals; D014812:Vitamin K; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D010447:Peptide Hydrolases; D005974:Glutamate-Ammonia Ligase; D010100:Oxygen