Biotinylated DNA probes for exotoxin A and pilin genes in the differentiation of Pseudomonas aeruginosa strains.
Biotin-labeled DNA probes derived from Pseudomonas aeruginosa exotoxin A and pilin genes were tested for their ability to distinguish strains among a selected group of P. aeruginosa isolates. Probing of Southern blots of restriction digests of DNA from test strains with the exotoxin A probe demonstrated a unique hybridization pattern for each independently isolated strain containing the exotoxin A gene. Two phenotypically distinct strains isolated from the same patient were found to be identical in their DNA hybridization patterns. By using a pilin gene probe, similar distinction was made between independent strains, while strains from the same source were confirmed to be identical. Furthermore, DNA from a strain of P. aeruginosa lacking the exotoxin A gene yielded a unique pattern of restriction fragments which hybridized to the pilin gene probe. The exotoxin A and the pilin probes may together prove to be useful tools in epidemiological surveys during outbreaks of P. aeruginosa infection.
Samadpour
M
M
Department of Microbiology, School of Medicine, University of Washington, Seattle 98195.
Moseley
S L
SL
Lory
S
S
D036002:ADP Ribose Transferases; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D001710:Biotin; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005098:Exotoxins / Q000235:genetics*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial; D005796:Genes; D005798:Genes, Bacterial; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics*; D015183:Restriction Mapping; D013045:Species Specificity; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D001710:Biotin; D015342:DNA Probes; D004269:DNA, Bacterial; D005098:Exotoxins; D036781:Fimbriae Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A