Induction and characterization of mitochondrial DNA mutants in Chlamydomonas reinhardtii.
In addition to lethal minute colony mutations which correspond to loss of mitochondrial DNA, acriflavin induces in Chlamydomonas reinhardtii a low percentage of cells that grow in the light but do not divide under heterotrophic conditions. Two such obligate photoautotrophic mutants were shown to lack the cyanide-sensitive cytochrome pathway of the respiration and to have a reduced cytochrome c oxidase activity. In crosses to wild type, the mutations are transmitted almost exclusively from the mating type minus parent. A same pattern of inheritance is seen for the mitochondrial DNA in crosses between the two interfertile species C. reinhardtii and Chlamydomonas smithii. Both mutants have a deletion in the region of the mitochondrial DNA containing the apocytochrome b gene and possibly the unidentified URFx gene.
Matagne
R F
RF
Département de Botanique, Université de Liège, Sart Tilman, Belgium.
Michel-Wolwertz
M R
MR
Munaut
C
C
Duyckaerts
C
C
Sluse
F
F
D000167:Acriflavine / Q000494:pharmacology; D002696:Chlamydomonas / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D003433:Crosses, Genetic; D004272:DNA, Mitochondrial / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D010101:Oxygen Consumption; D015183:Restriction Mapping
D004272:DNA, Mitochondrial; D000167:Acriflavine