title: Heavy water (D2O)-induced shape changes, movements and F-actin redistribution in human neutrophil granulocytes.
abstract: Heavy water (D2O) induces characteristic shape changes and a distinct type of movement in human neutrophil granulocytes. In contrast to front-tail polarity as evoked by chemotactic peptides and microtubule-disassembling agents, D2O-based media produce non-polar neutrophils with many small or long surface projections. This phenotype is similar to that elicited by both phorbol myristate acetate and diacylglycerols, but the surface projections are smaller and more densely placed and are often associated with a single large projection. D2O-induced non-polar cells with surface projections perform continuous shape changes without front-tail polarity and without the unidirectional movement and cytoplasmic streaming seen in cells with front-tail polarity. Some of the cells show circus movements of a large projection indicating circular polarity. In neutrophils suspended in D2O, F-actin is shifted to the cell periphery, mainly into the surface projections of activated cells. The D2O-induced effects are reversed in H2O-based medium. D2O is dominant over the chemotactic peptide, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), colchicine and taxol in that the combined action of D2O with any of these agents results in the D2O-induced phenotype. In contrast, cytochalasin B alone and in combination with fMLP induces a considerable decrease of non-polar cells and an increase of spherical cells similar to non-stimulated cells in H2O-based medium. Earlier studies indicated that D2O acts on microtubules. Our results suggest that D2O may act on the microfilament system. Neutrophils suspended in D2O-based medium may represent a useful model to study the relationship between shapes, movements, and particular functions of these cells.
authors.affiliation: Institute of Pathology, University of Bern, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Zimmermann
authors.forename: H U
authors.initials: HU
authors.lastname: Keller
authors.forename: H
authors.initials: H
authors.lastname: Cottier
mesh_terms: D000199:Actins / Q000097:blood*; D000470:Alkaloids / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects; D003903:Deuterium / Q000009:adverse effects*; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000032:analysis* / Q000166:cytology / Q000648:ultrastructure; D017239:Paclitaxel
chemical_list: D000199:Actins; D000470:Alkaloids; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003903:Deuterium; D017239:Paclitaxel; D003078:Colchicine