Detection of human lymphocyte subpopulations by using monoclonal antibodies and protein A-coated erythrocytes.
The results obtained by using the protein A-rosette assay for human peripheral blood lymphocytes treated with a series of monoclonal antibodies were very close to the estimates obtained by indirect fluorescence. The percentages of T3-, T4-, T8- and DR-positive cells (T3 = T lymphocytes, T4 = helper T lymphocytes, T8 = suppressor T lymphocytes, DR = B, L and null cells) in normal and pathological peripheral blood were found to vary within the limits reported by many other authors using different techniques for the identification of cells binding the specific monoclonal antibody.
Roman
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Moraru
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Ghetie
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D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000145:classification; D004912:Erythrocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006689:Hodgkin Disease / Q000097:blood; D006801:Humans; D007938:Leukemia / Q000097:blood; D008214:Lymphocytes / Q000145:classification* / Q000276:immunology; D008223:Lymphoma / Q000097:blood; D051379:Mice; D012397:Rosette Formation; D013205:Staphylococcal Protein A / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification; D050378:T-Lymphocytes, Regulatory / Q000145:classification
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D013205:Staphylococcal Protein A