title: Lack of effect of maternal age on UV-induced DNA repair in mouse oocytes.
abstract: Oocytes at the dictyate stage from young (8-14 weeks) and old (12-15 months) BALB/c mice were manually isolated and UV-irradiated. They were cultured for 1 h in medium containing tritiated thymidine and chased for a further hour in cold thymidine medium before being incubated for 18-20 h in medium with no added thymidine. Oocytes which had developed to metaphase II were analysed following autoradiography. Pooled results from 14 replicate experiments revealed no significant age-related difference between the mean corrected grain count per cell [159.2 +/- 8.5 (86 cells) for young mice and 164.6 +/- 9.8 (70 cells) for the old animals]. Thus in the female mouse the oocyte's capacity to repair UV-induced damage is apparently maintained at a high level throughout reproductive life.
authors.affiliation: Department of Genetics, Monash University, Clayton, Vic., Australia.
authors.forename: C L
authors.initials: CL
authors.lastname: Guli
authors.forename: D R
authors.initials: DR
authors.lastname: Smyth
mesh_terms: D000818:Animals; D004260:DNA Repair / Q000528:radiation effects*; D008423:Maternal Age; D008540:Meiosis / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009865:Oocytes / Q000528:radiation effects*; D014466:Ultraviolet Rays