title: Coproporphyrinogen oxidase, protoporphyrinogen oxidase and ferrochelatase activities in human liver biopsies with special reference to alcoholic liver disease.
abstract: The activities of coproporphyrinogen oxidase, protoporphyrinogen oxidase and ferrochelatase have been assayed in human liver biopsies using recently developed highly sensitive specific enzyme assays. The specific activities (nmol/min/mg protein) in controls were 0.010 +/- 0.003 (mean +/- S.D., n = 11), 0.18 +/- 0.07 (n = 9) and 0.062 +/- 0.022 (n = 8), respectively. The total activities (mumol/min/liver) were determined, using ultrasound to determine liver volumes, and were 2.6 +/- 0.6 (n = 5), 36.6 +/- 13.9 (n = 6) and 14.2 +/- 5.4 (n = 3), respectively. Both specific and total enzyme activities in alcoholics with fatty liver were not significantly different from normal controls. Decreased protoporphyrinogen oxidase activity (0.08 nmol/min/mg protein or 20.2 mumol/min/liver) was found in two patients with variegate porphyria. In a patient with erythrohepatic protoporphyria a reduction of the ferrochelatase activity (0.01 nmol/min/mg protein) was demonstrated.
authors.affiliation: Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, U.K.
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.forename: C K
authors.initials: CK
authors.lastname: Lim
authors.forename: K J
authors.initials: KJ
authors.lastname: Simpson
authors.forename: T J
authors.initials: TJ
authors.lastname: Peters
mesh_terms: D001706:Biopsy; D003304:Coproporphyrinogen Oxidase / Q000378:metabolism*; D005294:Ferrochelatase / Q000378:metabolism*; D005420:Flavoproteins; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000201:enzymology* / Q000473:pathology; D008190:Lyases / Q000378:metabolism*; D024101:Mitochondrial Proteins; D010088:Oxidoreductases / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D050768:Protoporphyrinogen Oxidase; D012016:Reference Values
chemical_list: D005420:Flavoproteins; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D003304:Coproporphyrinogen Oxidase; C493978:PPOX protein, human; D050768:Protoporphyrinogen Oxidase; D008190:Lyases; D005294:Ferrochelatase