Antibody-antigen binding in organic solvents.
We describe, for the first time, the action of antibodies in anhydrous organic solvents. It has been demonstrated that the binding of a hapten, 4-aminobiphenyl, to the immobilized monoclonal antibody 2E11 is strong and specific not only in water but also in a variety of non-aqueous media. Further, the strength of interaction between antibody and hapten has been related to the hydrophobicity of the solvent: the more hydrophobic the solvent, the weaker the protein-ligand interaction.
Russell
A J
AJ
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
Trudel
L J
LJ
Skipper
P L
PL
Groopman
J D
JD
Tannenbaum
S R
SR
Klibanov
A M
AM
D000611:Aminobiphenyl Compounds; D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D005260:Female; D006241:Haptens; D051379:Mice; D008815:Mice, Inbred Strains; D011863:Radioimmunoassay; D012997:Solvents
D000611:Aminobiphenyl Compounds; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006241:Haptens; D012997:Solvents; C006757:4-biphenylamine