In vitro study of adrenergic stimulation of 32P incorporation into phospholipids of brown adipose tissue of control and cold-acclimated rats.
1. 32P-labelled inorganic phosphate incorporation into total and mitochondrial phospholipids was studied, in vitro, on brown adipose tissue (BAT) of control and cold-acclimated rats. 2. It was found that norepinephrine acts as in vivo, on BAT phospholipid metabolism via alpha 1 adrenergic receptors specifically increasing phosphatidic acid and phosphatidylinositol turnover with the same magnitude in both groups. 3. Cold-induced alpha 1 adrenergic desensitization is not as important as cold-induced beta adrenergic desensitization. 4. No specific effect of norepinephrine was seen in mitochondrial phospholipid turnover.
Senault
C
C
Laboratoire d'Adaptation Energétique à l'Environnement, E.P.H.E., Collège de France.
Meister
R
R
Portet
R
R
D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003081:Cold Climate; D066298:In Vitro Techniques; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D051381:Rats
D010710:Phosphates; D010743:Phospholipids; D010656:Phenylephrine; D009638:Norepinephrine