title: The human 32-kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase.
abstract: Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS-polyacrylamide gel electrophoresis of [35S]methionine-labeled cellular proteins indicated that these treatments also resulted in the induction of a 32-kDa protein. Immunoblot analysis further showed that the 32-kDa protein reacted with anti-bovine heme oxygenase antibodies. Treatment of the cells with cobaltic chloride or heat induced neither the 32-kDa protein nor heme oxygenase activity. It is concluded that the 32-kDa stress protein induced by arsenite and cadmium ions in these human cells is heme oxygenase.
authors.affiliation: Department of Hygiene, Kansai Medical University, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Taketani
authors.forename: H
authors.initials: H
authors.lastname: Kohno
authors.forename: T
authors.initials: T
authors.lastname: Yoshinaga
authors.forename: R
authors.initials: R
authors.lastname: Tokunaga
mesh_terms: D000838:Anions; D001151:Arsenic / Q000494:pharmacology*; D018053:Arsenites; D002104:Cadmium / Q000494:pharmacology*; D002412:Cations; D003035:Cobalt / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D007938:Leukemia; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D008970:Molecular Weight; D014407:Tumor Cells, Cultured
chemical_list: D000838:Anions; D018053:Arsenites; D002412:Cations; D006360:Heat-Shock Proteins; D002104:Cadmium; D003035:Cobalt; D006899:Mixed Function Oxygenases; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C018021:cobaltous chloride; C015001:arsenite; D001151:Arsenic