Rotation of the cell nucleus in living cells: a quantitative analysis.
Nuclear rotation is observed in a variety of cell types. However, few quantitative analyses are reported and the significance of this phenomenon is still unclear. To investigate this type of nuclear movement, we performed a quantitative analysis in mouse L-929 fibroblasts, a cell line chosen since it displays a high nuclear rotational activity. Analyses were performed using time-lapse microcinematography. The relationship between nuclear rotation and other cellular phenomena such as the cell cycle and locomotion were studied. Then, we investigated the rotation in a population of sister cells to study whether it is genetically determined. Finally, we performed a qualitative analysis of nuclear rotation in different cultured cell lines. Results show that nuclear rotations preferentially occur during the phases of the cell cycle which surround mitosis.
Bard
F
F
Bourgeois
C A
CA
Costagliola
D
D
Bouteille
M
M
D000818:Animals; D002452:Cell Count; D002465:Cell Movement; D002467:Cell Nucleus / Q000502:physiology*; D007399:Interphase; D007739:L Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008858:Microscopy, Phase-Contrast; D010784:Photomicrography; D013997:Time Factors