Microcycle conidiation in submerged cultures of Penicillium cyclopium attained without temperature changes.
Microcycle conidiation in shaken cultures of Penicillium cyclopium M 79 was induced at 24 degrees C without any shock treatment. The occurrence of a microcycle depended on the presence of an organic acid (especially glutamic acid) in combination with glucose, low phosphate concentration, light and sufficient aeration. Absence of glucose and/or lowered aeration evoked vegetative growth. A synchronous and homogeneous microcycle required a certain relationship between the number of inoculated conidia and the concentration of the organic acid in the medium; the optimum was at 0.08 nmol acid per conidium. Higher values stimulated normal vegetative growth. A shortage or absence of the organic acid led to an atypical growth. The effect of organic acid can be partially replaced by addition of 2% (w/v) CaCO3. Addition of NH+4 in concentrations higher than 6 mM to cultures with glutamate or glutamine evoked vegetative growth.
Pazout
J
J
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Schröder
P
P
D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006863:Hydrogen-Ion Concentration; D010407:Penicillium / Q000187:drug effects / Q000254:growth & development*; D010710:Phosphates; D013696:Temperature
D003470:Culture Media; D005971:Glutamates; D010710:Phosphates; D018698:Glutamic Acid; D005947:Glucose