Biosynthesis of the cancer-associated sialyl-Lex determinant in human amniotic fluid.
Biosynthesis of the sialyl-Lex determinant (NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)-GlcNAc beta 1-3-R) in human amniotic fluid has been shown to proceed via the same sequence of glycosylation steps established previously for lung carcinoma PC 9 cells (Holmes, E. H., Ostrander, G.K. & Hakomori, S. (1986) J. Biol. Chem. 261, 3737-3743): sialylation of type-2-chain-precursor substrates (paragloboside) by an amniotic alpha 2-3-sialyltransferase precedes fucosylation of sialylated intermediates (sialosyl paragloboside) by an organ-characteristic alpha 1-3-L-fucosyltransferase.
Mitsakos
A
A
Institut für Immunbiologie, Medizinische Universitätsklinik Köln.
Hanisch
F G
FG
Uhlenbruck
G
G
D000653:Amniotic Fluid / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006017:Glycolipids / Q000096:biosynthesis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016256:Lewis X Antigen; D011247:Pregnancy; D012799:Sialyltransferases / Q000378:metabolism*
D006017:Glycolipids; D016256:Lewis X Antigen; D012799:Sialyltransferases