Techniques for mitochondrial and ribosomal DNA analysis of anopheline mosquitoes.
Methods are described for the isolation of mitochondrial and total cellular DNA from mosquitoes. The mitochondrial and ribosomal DNA restriction patterns could be detected in the total DNA of an individual mosquito by the use of cloned probes. DNA restriction analysis may prove to be a useful alternative to isozyme electrophoresis for the study of insect population genetics.
Cockburn
A F
AF
USDA, ARS, Insects Affecting Man and Animals Research Laboratory, Gainesville, FL 32604.
Seawright
J A
JA
D000818:Animals; D000852:Anopheles / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D015342:DNA Probes; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal