title: Purification of carp growth hormone and cloning of the complementary DNA.
abstract: The growth hormone (GH) was isolated and purified from common carp (Cyprinus carpio) pituitary glands by salt precipitation and HPLC on reverse-phase C18 columns. The carp GH cDNA was synthesized and cloned in Escherichia coli using EcoRI linkers and pBR322 as vector. The positive clones were selected and sequenced. The full-length carp GH cDNA contains 1187 nucleotide basepairs with an open reading frame coding for the precursor form carp GH of 210 amino-acid residues. The partial amino-acid sequence from the protein completely agrees with that derived from the cDNA, with serine as the first residue in mature carp GH preceded by a 22-residue hydrophobic signal peptide. Comparison of the amino-acid sequence of carp GH with those of various species reveals positional identity at 32.4%, 38.8%, 42.0%, 37.2%, 66%, 55% and 49% with GHs of man, rat, duck, bullfrog, salmon, tuna and yellow tail, respectively.
authors.affiliation: Institute of Biochemical Sciences, National Taiwan University, Taipei, China.
authors.forename: S C
authors.initials: SC
authors.lastname: Chao
authors.forename: F M
authors.initials: FM
authors.lastname: Pan
authors.forename: W C
authors.initials: WC
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics*; D004247:DNA / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping
chemical_list: D013006:Growth Hormone; D004247:DNA