A new ultraviolet-light sensitive mutant of Neurospora crassa with unusual photoreactivation property.
A mutant, uvs-(SA3B), which shows high sensitivity to UV light segregated among the progeny in a back-cross of a presumptive MMS-sensitive mutant to a wild-type strain. At 37% survival, this mutant was approximately 5 times more sensitive to UV and also 6 times more sensitive to 4-NQO than the wild type. But it was only slightly sensitive to gamma-ray, MMS, MNNG, MTC and histidine. It showed an unusual photoreactivation response. Its time course of photorecovery was similar to the photoreactivation-defective strain upr-1 of Neurospora crassa. Mutation induction by UV at the ad-3 loci in this mutant strain was lower than that at the same loci in the wild-type strain. The uvs-(SA3B) mutant maps between met-1 and col-4 in linkage group IV, and it was not allelic with the mutagen-sensitive mutant mus-8 which is located in this area. We have concluded, therefore, that uvs-(SA3B) has resulted from mutation in a new DNA-repair gene. This new mutant was barren in homozygous crosses.
Inoue
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Ishii
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D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology*; D005720:Gamma Rays; D008040:Genetic Linkage; D005838:Genotype; D006639:Histidine / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009152:Mutagenicity Tests; D009154:Mutation; D009491:Neurospora / Q000528:radiation effects*; D009492:Neurospora crassa / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D013045:Species Specificity; D014466:Ultraviolet Rays
D002273:Carcinogens; D008937:Mitomycins; D008769:Methylnitronitrosoguanidine; D006639:Histidine; D016685:Mitomycin; D015112:4-Nitroquinoline-1-oxide; D008741:Methyl Methanesulfonate