beta-Methyl-15-p-iodophenylpentadecanoic acid metabolism and kinetics in the isolated rat heart.
The use of 15-p-iodophenyl-beta-methyl-pentadecanoic acid (beta Me-IPPA) as an indicator of long chain fatty acid (LCFA) utilization in nuclear medicine studies was evaluated in the isolated, perfused, working rat heart. Time courses of radioactivity (residue curves) were obtained following bolus injections of both beta Me-IPPA and its straight chain counterpart 15-p-iodophenyl-pentadecanoic acid (IPPA). IPPA kinetics clearly indicated flow independent impairment of fatty acid oxidation caused by the carnitine palmitoyltransferase I inhibitor 2[5(4-chlorophenyl)pentyl]oxirane-2-carboxylate (POCA). In contrast, beta Me-IPPA kinetics were insensitive to changes in fatty acid oxidation rate and net utilization of long chain fatty acid. Analysis of radiolabeled species in coronary effluent and heart homogenates showed the methylated fatty acid to be readily incorporated into complex lipids but a poor substrate for oxidation. POCA did not significantly alter metabolism of the tracer, suggesting that the tracer is poorly metabolized beyond beta Me-IPPA-CoA in the oxidative pathway.
DeGrado
T R
TR
Department of Medical Physics, University of Wisconsin, Madison 53706.
Holden
J E
JE
Ng
C K
CK
Raffel
D M
DM
Gatley
S J
SJ
D000818:Animals; D005227:Fatty Acids / Q000378:metabolism; D006321:Heart / Q000000981:diagnostic imaging*; D007457:Iodine Radioisotopes; D007462:Iodobenzenes / Q000493:pharmacokinetics; D009206:Myocardium / Q000378:metabolism; D011877:Radionuclide Imaging; D051381:Rats; D013329:Structure-Activity Relationship
D005227:Fatty Acids; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; C025391:omega-(4-iodophenyl)pentadecanoic acid