Histidine modulates the clastogenic effect of oxidative stress.
The kinetics of the formation of single-strand breaks induced by H2O2 in DNA is more rapid in Eagle's Modified Minimal Essential Medium (MEM) than in phosphate-buffered saline (PBS). Among the components of MEM, we found that histidine increases the rate of degradation of DNA by H2O2 in PBS dose-dependently. In hamster lung fibroblasts histidine increases the cytotoxicity of H2O2, as well as the number of sister-chromatid exchanges and the frequency of micronuclei induced by hydrogen peroxide.
Tachon
P
P
Laboratoires de Recherche Fondamentale de L'Oréal, Aulnay sous Bois, France.
Giacomoni
P U
PU
D000818:Animals; D002021:Buffers; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004278:DNA, Superhelical / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006639:Histidine / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007700:Kinetics; D008168:Lung; D015162:Micronucleus Tests; D012854:Sister Chromatid Exchange / Q000187:drug effects
D002021:Buffers; D004278:DNA, Superhelical; D006639:Histidine; D006861:Hydrogen Peroxide