An investigation of allogeneic pregnancy in multiparous mice subjected to in vivo depletion of CD8 (Ly2)-positive lymphocytes by monoclonal antibody treatment.
Adult thymectomized C57/Bl (H-2b) and DBA/1 (H-2q) female mice were subjected to treatment with rat anti-mouse CD8 and mouse anti-rat Ig (kappa) prior to entering their third pregnancy with CBA/Ca (H-2k) males. The treatment protocol drastically reduced the number of CD8 (Ly2)-carrying lymphocytes (T-cytotoxic/suppressor phenotype) in the spleen and para-aortic lymph nodes, as assessed by immuno-staining. All mice were investigated on day 18 of their third gestation. The following data were collected from experimental and control groups: (1) resorption frequency, (2) weight of the placenta, fetuses, spleen and para-aortic lymph nodes, (3) immunohistochemical analysis of maternal lymphoid tissues, (4) level of anti-paternal IgG serum antibodies, (5) content of "background" IgM and IgG-secreting cells in spleen and para-aortic lymph nodes. Neither the resorption frequency nor placental/fetal weight was affected by anti-CD8 treatment. However, the formation of anti-paternal antibodies was enhanced in anti-CD8 treated C57/Bl mice.
Sulila
P
P
Department of Zoophysiology, University of Uppsala, Sweden.
Holmdahl
R
R
Hansson
I
I
Bernadotte
F
F
Mattsson
A
A
Mattsson
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D000921:Antibody-Producing Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D016827:CD8 Antigens; D005260:Female; D007518:Isoantibodies / Q000096:biosynthesis; D008212:Lymphocyte Depletion; D008221:Lymphoid Tissue / Q000033:anatomy & histology; D051379:Mice; D008815:Mice, Inbred Strains; D010298:Parity; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D016827:CD8 Antigens; D007518:Isoantibodies