title: Influence of neurons on the occurrence of fibre types and myosin light chains in cultured presumptive slow and fast myoblasts.
abstract: Myoblasts from 9-day-old quail embryo slow anterior latissimus dorsi (ALD) and fast posterior and latissimus dorsi (PLD) muscles were co-cultured with neurons. The presence of neurons allowed ALD-derived muscle fibres to express characteristic features of a slow muscle (occurrence of alpha' and of beta' fibres and predominance of slow myosin light chains). On the contrary, PLD-derived fibres did not differentiate into normal fast fibres (occurrence of alpha'-like fibres and absence of LC3f). These results are compared with the differentiation of ALD and PLD myoblasts in aneural condition. It is suggested that neurons can modify some phenotypic expression of presumptive slow or fast myoblasts.
authors.forename: P H
authors.initials: PH
authors.lastname: Fremont
authors.forename: M F
authors.initials: MF
authors.lastname: Gardahaut
authors.forename: T
authors.initials: T
authors.lastname: Rouaud
authors.forename: D
authors.initials: D
authors.lastname: Renaud
authors.forename: G H
authors.initials: GH
authors.lastname: Le Douarin
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian; D009132:Muscles / Q000032:analysis / Q000166:cytology* / Q000201:enzymology / Q000294:innervation; D009210:Myofibrils / Q000201:enzymology; D009218:Myosins / Q000032:analysis*; D009246:NADH Tetrazolium Reductase / Q000032:analysis; D009474:Neurons / Q000502:physiology*; D011784:Quail
chemical_list: D009246:NADH Tetrazolium Reductase; D000251:Adenosine Triphosphatases; D009218:Myosins