title: Characterization of very low density lipoprotein from Watanabe heritable hyperlipidemic rabbits.
abstract: We investigated the properties of very low density lipoprotein (VLDL) from two types of Watanabe heritable hyperlipidemic (WHHL) rabbits: one with a high incidence of coronary atherosclerosis (type 1), and the other with a low incidence (type 2). When incubated with mouse peritoneal macrophages, VLDL from type 1 WHHL rabbit (type 1-VLDL) stimulated cholesteryl ester synthesis 10.5-fold more than VLDL from the type 2 WHHL rabbit (type 2-VLDL) did. Moreover, a similar difference was seen in the stimulation of cholesteryl ester synthesis in peritoneal macrophages isolated from the WHHL rabbits. The mass ratios of cholesterol to protein in type 1- and type 2-VLDL were 5.69 and 2.05, respectively. Agarose gel electrophoresis of type 1-VLDL showed beta mobility, and that of type 2-VLDL showed pre-beta mobility. No difference was seen between the sizes of VLDL particles of the two types. The amount of apolipoprotein E in type 1-VLDL was greater than that in type 2-VLDL. In conclusion, the difference between type 1 and type 2 WHHL rabbits is at least partly due to the presence in type 1 animals of VLDL particles rich in cholesteryl esters and apolipoprotein E, particles which are very similar to beta-VLDL in conformation.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ishii
authors.forename: T
authors.initials: T
authors.lastname: Kita
authors.forename: M
authors.initials: M
authors.lastname: Yokode
authors.forename: N
authors.initials: N
authors.lastname: Kume
authors.forename: Y
authors.initials: Y
authors.lastname: Nagano
authors.forename: H
authors.initials: H
authors.lastname: Otani
authors.forename: T
authors.initials: T
authors.lastname: Yamamura
authors.forename: S
authors.initials: S
authors.lastname: Murayama
authors.forename: Y
authors.initials: Y
authors.lastname: Morimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Teranishi
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000032:analysis; D002788:Cholesterol Esters / Q000096:biosynthesis*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004587:Electrophoresis, Agar Gel; D005260:Female; D006949:Hyperlipidemias / Q000097:blood*; D007525:Isoelectric Focusing; D008079:Lipoproteins, VLDL / Q000097:blood*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D011817:Rabbits
chemical_list: D001057:Apolipoproteins E; D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; D008079:Lipoproteins, VLDL