Behaviour of several enzymes of fetal chick intestine in tissue culture.
The behaviour of several enzymes is described of the fetal chick duodenum in tissue culture in a defined medium free of serum and hormones. During culture the activity of sucrase, maltase, alanine aminopeptidase, and gamma-glutamyltransferase is raised in tissue explants, whereas the activity of other enzymes (dipeptidyl peptidase IV, leucine amino-peptidase, alkaline phosphatase) remains constant. After culture, depending on the enzyme, a varying amount of activity is found in the medium, a part of which can be sedimented by ultracentrifugation. Sucrase is subject to the strongest increase in activity during culture and thus should represent a sensitive marker for investigating maturation processes in the fetal intestine and their disturbances.
Mothes
T
T
Institute of Pathological Biochemistry, Karl Marx University, Leipzig.
Rüdiger
C
C
Mühle
W
W
Hütter
H J
HJ
D000818:Animals; D002642:Chick Embryo; D004386:Duodenum / Q000196:embryology / Q000201:enzymology*; D006867:Hydrolases / Q000378:metabolism*; D009924:Organ Culture Techniques; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D005723:gamma-Glutamyltransferase; D006867:Hydrolases