Metabolic correction of defects in the lipid anchoring of Thy-1 in lymphoma mutants.
Many plasma membrane proteins, including Thy-1, are anchored by a carboxyl terminal glycophospholipid. This unit is absent from the Thy-1 of several lymphoma mutants that synthesize the Thy-1 polypeptide but fail to express it at the cell surface. Recessive mutants of complementation groups A to C, E, and F contain Thy-1 mRNA of normal size, which suggests that their Thy-1 polypeptide is normal. To identify possible metabolic lesions, each mutant was grown with various supplements. The class F and B mutants exhibited a reversible induction of surface lipid anchored Thy-1 when grown with the aminoglycoside G418. Other aminoglycosides, sugars, and ethanolamine were inactive. These unexpected observations are discussed in the context of lipid anchor biosynthesis.
Gupta
D
D
Department of Pathology, Case Western Reserve University, Cleveland, OH 44106.
Tartakoff
A
A
Tisdale
E
E
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000954:Antigens, Surface / Q000235:genetics*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology; D005839:Gentamicins / Q000494:pharmacology; D006031:Glycosylation; D008223:Lymphoma / Q000235:genetics* / Q000276:immunology; D008563:Membrane Lipids / Q000502:physiology*; D009154:Mutation; D010743:Phospholipids / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D018800:Thy-1 Antigens
D000900:Anti-Bacterial Agents; D000954:Antigens, Surface; D005839:Gentamicins; D008563:Membrane Lipids; D010743:Phospholipids; D012333:RNA, Messenger; D018800:Thy-1 Antigens; C010680:antibiotic G 418