Studies on the pharmacokinetics of chlorambucil and prednimustine in patients using a new high-performance liquid chromatographic assay.
Following the oral administration of either chlorambucil/prednisolone or prednimustine to patients, the plasma levels of free chlorambucil and phenylacetic acid mustard, the beta-oxidation product of chlorambucil, were measured using a new high-performance liquid chromatographic (HPLC) assay. This assay permitted the simultaneous detection of the analyzed compounds with a lower limit of detection of 30 ng/ml. The pharmacokinetics of chlorambucil and phenylacetic acid mustard were found to be entirely different when prednimustine was administered as opposed to its components chlorambucil and prednisolone together. After the ingestion of the conjugate, the plasma concentration-time curves of chlorambucil and phenylacetic acid mustard showed a "delayed" pattern compared with those obtained after the administration of the components. The mean area under the concentration-time curves (AUCs) of prednimustine-derived chlorambucil and phenylacetic acid mustard were 25% and 40%, respectively, of the areas obtained after a stoichiometrically equivalent dose of chlorambucil. Free plasma prednimustine could not be detected at any time. This different pharmacokinetic behavior might offer an explanation for the superior therapeutic effects of prednimustine demonstrated by clinical studies.
Oppitz
M M
MM
Department of Internal Medicine, University of Bonn, Federal Republic of Germany.
Musch
E
E
Malek
M
M
Rüb
H P
HP
von Unruh
G E
GE
Loos
U
U
Mühlenbruch
B
B
D002699:Chlorambucil / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004338:Drug Combinations; D006801:Humans; D009588:Nitrogen Mustard Compounds / Q000097:blood; D011238:Prednimustine / Q000097:blood / Q000493:pharmacokinetics*; D011239:Prednisolone / Q000097:blood / Q000493:pharmacokinetics; D013997:Time Factors
D004338:Drug Combinations; D009588:Nitrogen Mustard Compounds; C008826:phenacid; D002699:Chlorambucil; D011238:Prednimustine; D011239:Prednisolone