Co-localization of tyrosine hydroxylase (TH)- and serotonin (5-HT)-immunoreactive innervation in the rat pituitary gland.
Adult male Sprague-Dawley rats were examined for possible co-localization of tyrosine hydroxylase (TH) and serotonin (5-HT) within innervation of the pituitary neurointermediate lobe. Use of sheep antiserum to TH and a secondary antibody coupled to fluorescein isothiocyanate (FITC), followed by rabbit anti-5-HT, then rhodamine-coupled second antibody, produced co-existent staining in some, but not all fibers of both the neural and intermediate lobes, using paraffin embedded tissues. Application of a combination of the two primary antibodies followed by sequential application of secondary antibody also resulted in co-localized antigens in selected fibers. Multiple 'classic' neurotransmitters within the same nerve terminals may modulate selected areas of endocrine tissue to control hormone release.
Saland
L C
LC
Department of Anatomy, University of New Mexico School of Medicine, Albuquerque 87131.
Wallace
J A
JA
Samora
A
A
Gutierrez
L
L
D000818:Animals; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D008297:Male; D010902:Pituitary Gland / Q000166:cytology / Q000201:enzymology / Q000294:innervation*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis*; D013861:Thiocyanates; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D012701:Serotonin; D014446:Tyrosine 3-Monooxygenase; D016650:Fluorescein-5-isothiocyanate