Regional mapping of unique DNA sequences from human chromosome 3 derived from a flow-sorted chromosome library.
Eight single-copy DNA probes specific for human chromosome 3 were isolated by screening a human chromosome 3-derived genomic library. Southern blot analyses of DNAs isolated from a panel of somatic cell hybrids allowed us to regionally assign all probes to subregions on chromosome 3. Three clones were localized to the short arm of chromosome 3 (3p21----pter), two to the long arm (3q21----qter), and three to the 3q21----3p21 subregion. Six of these DNA sequences map to regions overlapping a segment of chromosome 3 (3p14----p23) frequently deleted in small cell lung cancer cells. Restriction fragment length polymorphism analyses indicate that at least three of the eight single-copy probes studies show MspI or BglII polymorphisms. This library is a useful source of chromosome 3-specific probes.
Atchison
L M
LM
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111.
Naylor
S L
SL
Freed
J J
JJ
Comis
R L
RL
D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D015342:DNA Probes; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes