title: Effects of a triazine antifolate (NSC 127755) on serine hydroxymethyltransferase in myeloma cells in culture.
abstract: The effect of the triazine antifolate NSC 127755 on serine hydroxymethyltransferase activity in mouse myeloma X63 cells in culture was determined. The enzyme was inhibited, apparently irreversibly, with IC50 approximately 5 X 10(-8) M. A similar concentration-dependency for inhibition of [6-3H]deoxyuridine incorporation into DNA was observed in these cells in culture. Long-term culture of myeloma cells with NSC 127755 resulted in a progressive decrease in the number of viable cells. The study emphasises the significance of serine hydroxymethyltransferase as a target for anticancer chemotherapy.
authors.affiliation: Department of Biochemistry, University of Surrey, Guildford, U.K.
authors.forename: K
authors.initials: K
authors.lastname: Snell
authors.forename: D
authors.initials: D
authors.lastname: Riches
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005493:Folic Acid Antagonists / Q000494:pharmacology*; D012696:Glycine Hydroxymethyltransferase / Q000037:antagonists & inhibitors*; D051379:Mice; D009101:Multiple Myeloma / Q000201:enzymology*; D014166:Transferases / Q000037:antagonists & inhibitors*; D014227:Triazines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D005493:Folic Acid Antagonists; D014227:Triazines; C031776:NSC 127755; D014166:Transferases; D012696:Glycine Hydroxymethyltransferase