title: A new locus (NIA 1) in Arabidopsis thaliana encoding nitrate reductase.
abstract: We have isolated two nitrate reductase genes and their corresponding cDNAs from Arabidopsis thaliana. Sequences of the two cDNAs, when compared to a sequence of a barley cDNA clone, confirm their identity as nitrate reductase clones and show that they are closely related. The two genes have been mapped using restriction fragment length polymorphisms; gNR2 is close to the previously identified chl-3 locus and is probably identical to it, while gNR1 maps to a new locus (NIA1) on chromosome 1, near gl-2.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA.
authors.forename: C L
authors.initials: CL
authors.lastname: Cheng
authors.forename: J
authors.initials: J
authors.lastname: Dewdney
authors.forename: H G
authors.initials: HG
authors.lastname: Nam
authors.forename: B G
authors.initials: BG
authors.lastname: den Boer
authors.forename: H M
authors.initials: HM
authors.lastname: Goodman
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011233:Precipitin Tests; D015183:Restriction Mapping
chemical_list: D004247:DNA; D009565:Nitrate Reductases; D050901:Nitrate Reductase