Turbidimetric latex immunoassay of placental lactogen on microtiter plates.
In this latex immunoassay for human placental lactogen, microtiter plates are used as the reaction vessel and the absorbance at 405 nm is measured to quantify the reactions. This 30-min assay necessitates only one serum dilution and two pipetting steps. The calibration curve extends from 0.5 to 15 mg/L. CVs range from 4.2% to 7.0% for within-run determination and from 7.0% to 11.2% for between-run determinations. A correlation coefficient of 0.949 was obtained for 84 sera when the method was compared with a commercial radioimmunoassay.
Collet-Cassart
D
D
Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Limet
J N
JN
Van Krieken
L
L
De Hertogh
R
R
D005260:Female; D006801:Humans; D007118:Immunoassay; D007841:Latex Fixation Tests; D010928:Placental Lactogen / Q000097:blood*; D011247:Pregnancy; D011786:Quality Control; D011863:Radioimmunoassay; D012016:Reference Values; D013053:Spectrophotometry
D010928:Placental Lactogen