Perfluorinated acids as ion-pairing agents in the determination of monoamine transmitters and some prominent metabolites in rat brain by high-performance liquid chromatography with amperometric detection.
The behaviour of trifluoroacetate and heptafluorobutyrate as pairing ions for the reversed-phase ion-pair separation of monoamine transmitters and related metabolites was studied. The performance of systems with the perfluorinated acids was compared with that of systems containing sodium octyl sulphonate and was found to be better in terms of peak resolution combined with total analysis time, day-to-day reproducibility and the time required for attaining initial chromatographic equilibrium. Rat brain samples were deproteinized in the acidified mobile phase, injected directly on to a high-performance liquid chromatographic column and quantitated using an amperometric detector. Sample run times were 6-8 min, at a relatively low flow-rate. The detection limits achieved are fairly uncommon with conventional bore columns. The two perfluorinated acids studied differ in the dominant mechanisms of ion-pair formation and show selectivity differences as a result.
Patthy
M
M
Institute for Drug Research, Budapest, Hungary.
Gyenge
R
R
D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005466:Fluorocarbons; D006863:Hydrogen-Ion Concentration; D008297:Male; D018377:Neurotransmitter Agents / Q000032:analysis*; D051381:Rats; D014269:Trifluoroacetic Acid
D015306:Biogenic Monoamines; D005466:Fluorocarbons; D018377:Neurotransmitter Agents; C033094:perfluorobutyric acid; D014269:Trifluoroacetic Acid