Attempts at the detection of antibodies to Newcastle disease virus by haemofusion-inhibition test.
Two modifications of a haemofusion-inhibition test (HFI-1 and HFI-2) were applied for the titration of antibodies to Newcastle disease virus (NDV) in chicken sera. Statistical analysis revealed a positive correlation of the HFI-1 antibody titres with those measured by the standard haemagglutination-inhibition (HI), virus neutralization (VN) and haemolysis-inhibition (HLI) tests. The same appeared true when the HFI-2 antibody titres were compared with the HI, VN, and HLI tests. Except for the several sera collected from birds immunized with formalin-inactivated vaccine, the HFI-2 antibody titres of individual serum samples were usually lower than those determined by HFI-1. The interpretation of these differences as well as some advantages and disadvantages of the proposed test are discussed.
Trybala
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Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Academy of Agriculture and Technology in Olsztyn, Poland.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002459:Cell Fusion; D002645:Chickens / Q000276:immunology; D004912:Erythrocytes; D007158:Immunologic Techniques; D009522:Newcastle disease virus / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D014760:Viral Fusion Proteins