Dopaminergic cells in the superior cervical ganglion of the rat: light and electron microscopic study using an antibody against dopamine.
So-called small intensely fluorescent (SIF) cells were visualized immunohistochemically using a newly developed anti-dopamine (DA) serum in the rat superior cervical ganglia (SCG), both light and electron microscopically. Specificity of the anti-DA serum was tested in a control test and enzyme-linked immunosorbent assay (ELISA). DA-immunoreactivity was found in a subpopulation of SIF cells, but not in principal neurons. Ultrastructurally, DA-immunoreactivity was noticed in the cytoplasmic matrix and vesicles of perikarya, dendritic processes and terminals of SIF cells. DA-labeled terminals made symmetrical synaptic contacts with unlabeled principal neurons. These findings provide a morphological basis for the role of dopaminergic SIF cells as interneurons.
Sakai
M
M
Department of Anatomy, School of Medicine, Fujita-Gakuen Health University, Toyoake, Japan.
Kani
K
K
Yoshida
M
M
Nagatsu
I
I
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D004298:Dopamine / Q000378:metabolism*; D005728:Ganglia, Sympathetic / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D004298:Dopamine