Depression of afferent arc of the in vivo cytotoxic T-cell immunity by bacterial lipopolysaccharides.
The afferent arc of the in vivo cytotoxic T-cell immunity assessed by second set rejection of ascitic allogeneic tumors was shown to be depressed by bacterial lipopolysaccharide (LPS) that was administered simultaneously with or 1 day before injection of allogeneic spleen cells as stimulators. Two different LPSs from Escherichia coli O55 and Klebsiella O3 displayed similar activities whereas dextran sulfate, concanavalin A, or poly A:U was not effective. Stimulator activities of allogeneic cells was not directly modified by LPS. Any definite suppressor activity on afferent or efferent arc of the T-cell response was not demonstrable in mice receiving LPS and allogeneic cells. Further, the LPS effect for immune depression was not diminished by whole body X-ray irradiation to the recipient at 300 R, which ablated the B-cell reactivity to LPS for polyclonal activation, or by treatment of the recipient with carrageenan, a known toxic agent to macrophages. It was suggested from these results that LPS suppresses the cytotoxic T-cell immunity by modulating responder T cells to be temporarily refractory to the allogeneic stimulus rather than by activating suppressor cells such as radiation-sensitive lymphocytes and carrageenan-sensitive macrophages.
Mizoguchi
K
K
Nakashima
I
I
Hasegawa
Y
Y
Isobe
K
K
Kato
N
N
Shimokata
K
K
Kawashima
K
K
Nagase
F
F
Ando
K
K
Yoshida
T
T
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects* / Q000528:radiation effects; D006084:Graft Rejection / Q000187:drug effects; D007116:Immunization, Passive; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D008223:Lymphoma / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008817:Mice, Mutant Strains; D011828:Radiation Chimera; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D002351:Carrageenan