A bioassay method for studying factors stimulating erythropoiesis using as recipient the mouse with a functionally active erythron.
This novel bioassay method basically differs from the conventional plethoric mouse assay in the timing of the onset of induced polycythemia relative to the administration of the substances that are being tested. While the latter measures the regenerating action of erythropoietin at a time when erythropoiesis is virtually absent, the method here described evaluates the stimulatory action(s) required to maintain the normality of the process. This fact opens new approaches for studying factors involved in the quantitative govern of erythropoiesis in the steady state.
Scaro
J L
JL
Carrera
M
M
Miranda
C
C
Martín
B
B
Torrejón
I
I
D000818:Animals; D001681:Biological Assay; D001854:Bone Marrow Cells; D004305:Dose-Response Relationship, Drug; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D011086:Polycythemia / Q000097:blood*
D004921:Erythropoietin