Developmentally regulated late mRNAs in the encystment of Physarum polycephalum plasmodia.
Physarum polycephalum plasmodia survive adverse conditions by transforming into encysted cells called spherules. In this work we analysed the developmentally regulated mRNAs from the late stages of spherulation. A cDNA library was constructed and four abundant mRNAs were identified. One of the mRNAs was present in trace amounts in early spherules, while the other three were found only in late spherules. A cDNA clone for one of the late spherulation specific mRNAs was sequenced. It codes for a 332-amino-acid protein that did not show significant similarities with any known protein. Since the mRNA for this protein accumulates during spherulation, the protein was called spherulin 4. This protein has many features of a plasma membrane protein; it contains a signal peptide and a long hydrophobic region, which could serve as a transmembrane anchor. Another interesting feature is the presence of seven consecutive glycine residues in the N-terminal region. This is even more remarkable since the protein is not rich in glycine.
Savard
L
L
Département de biologie, Faculté des Sciences, Université Laval, Québec, Canada.
Laroche
A
A
Lemieux
G
G
Pallotta
D
D
D000595:Amino Acid Sequence; D000946:Antigens, Fungal / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003046:Coccidioidin / Q000235:genetics*; D003062:Codon; D004247:DNA / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010804:Physarum / Q000502:physiology*; D012333:RNA, Messenger / Q000502:physiology*; D015183:Restriction Mapping; D012995:Solubility
D000946:Antigens, Fungal; D003062:Codon; D005656:Fungal Proteins; D012333:RNA, Messenger; C009303:spherulin; D003046:Coccidioidin; D004247:DNA