title: Role of BBM lipid composition and fluidity in impaired renal Pi transport in aged rat.
abstract: Renal tubular phosphate (Pi) transport is impaired in the aged rat. The decrement in Pi transport is manifested as a decrease in the Vmax of the luminal brush-border membrane (BBM) sodium-dependent Pi transport. In the present study we determined the potential role of alterations in cortical BBM lipid composition and fluidity in the age-related phosphaturia observed in rats. In the aged rat there are significant increases in the BBM cholesterol (Chol, 504 vs. 422 nmol/mg protein in adult, P less than 0.01) and sphingomyelin (Sph, 41.8 vs. 37.5 mol% in adult, P less than 0.01) and a decrease in the BBM fluidity [increase in fluorescence anisotropy of diphenylhexatriene (DPH), rDPH, 0.221 vs. 0.215 in adult, P less than 0.01]. The BBM lipid compositional and fluidity alterations may also play an important role in the impaired renal adaptation to a low-Pi diet in the aged rat. In the adult rat the renal adaptation to a low-Pi diet is associated with a decrease in BBM Chol (370 to 307 nmol/mg protein, P less than 0.01) and an increase in fluidity (decrease in rDPH 0.207 to 0.201, P less than 0.05). In the aged rat the renal adaptation to a low-Pi diet is incomplete and is associated with impaired ability to lower BBM Chol (441 to 429 nmol/mg protein, P = NS) and to increase fluidity (rDPH 0.211 to 0.211, P = NS). The results of this study therefore suggest that in the aged rat the increase in BBM Chol and Sph content, and the decrease in BBM fluidity play an important role in the impaired renal tubular Pi transport and adaptation to a low-Pi diet.
authors.affiliation: Department of Internal Medicine, Dallas Veterans Administration Medical Center, Texas.
authors.forename: M
authors.initials: M
authors.lastname: Levi
authors.forename: D M
authors.initials: DM
authors.lastname: Jameson
authors.forename: B W
authors.initials: BW
authors.lastname: van der Meer
mesh_terms: D000375:Aging; D000465:Algorithms; D000818:Animals; D001693:Biological Transport, Active; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis*; D008871:Microvilli / Q000032:analysis; D010710:Phosphates / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D008563:Membrane Lipids; D010710:Phosphates