Purification and characterization of acylation stimulating protein.
We have purified to homogeneity and analyzed the amino acid composition of a small (Mr 14,000), basic (pI 9.0) protein from human plasma. This has been named acylation stimulating protein (ASP) because it markedly stimulates triacylglycerol synthesis in human adipocytes. As well, it stimulates triacylglycerol synthesis in human skin fibroblasts cultured from normal individuals. Characteristic saturation curves for the cell metabolic responses to ASP were observed in both cell types with higher stimulation of oleate incorporation into triacylglycerol being observed in adipocytes. The stimulation of triacylglycerol synthesis was much greater with ASP than with insulin. Neither fatty acid binding protein nor albumin was able to mimic the ASP effect.
Cianflone
K M
KM
Cardiovascular Research Unit, Royal Victoria Hospital, Montreal, Quebec, Canada.
Sniderman
A D
AD
Walsh
M J
MJ
Vu
H T
HT
Gagnon
J
J
Rodriguez
M A
MA
D000273:Adipose Tissue / Q000378:metabolism; D001798:Blood Proteins / Q000302:isolation & purification*; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D015926:Complement C3a / Q000031:analogs & derivatives*; D005347:Fibroblasts / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis; D006801:Humans; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D012867:Skin / Q000378:metabolism
D001798:Blood Proteins; D005989:Glycerides; D009829:Oleic Acids; D010743:Phospholipids; C044541:complement C3a, des-Arg-(77)-; D019301:Oleic Acid; D015926:Complement C3a