Disassembly of the nuclear envelope of spisula oocytes in a cell-free system.
Nuclei isolated from oocytes of the surf clam Spisula solidissima are disassembled when exposed to extracts from maturing oocytes. In the course of this process the nuclear lamina undergoes a marked reduction in size and the nuclear membrane appears to be fragmented into vesicles. These events are accompanied by extensive phosphorylation of the oocyte 67-kDa lamin and its solubilization. The changes observed are similar to those which occur in vivo in activated Spisula oocytes. Nuclear envelope breakdown in vitro requires ATP and Mg2+, but not Ca2+. It is not affected by protease inhibitors and is inhibited by alkaline phosphatase.
Dessev
G
G
Department of Cell Biology and Anatomy, Northwestern University Medical School, Chicago, Illinois 60611.
Palazzo
R
R
Rebhun
L
L
Goldman
R
R
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000494:pharmacology; D000818:Animals; D049872:Bivalvia / Q000648:ultrastructure*; D002474:Cell-Free System; D002850:Chromatography, Gel; D034882:Lamins; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism; D017830:Octoxynol; D009865:Oocytes / Q000648:ultrastructure*; D010766:Phosphorylation; D011092:Polyethylene Glycols / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology
D034882:Lamins; D009687:Nuclear Proteins; D011480:Protease Inhibitors; D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D017830:Octoxynol; C010615:Nonidet P-40; D000469:Alkaline Phosphatase; D008274:Magnesium