Pulse-field linkage of the P3, G6pd and Cf-8 genes on the mouse X chromosome: demonstration of synteny at the physical level.
Utilising pulse-field gel electrophoresis physical linkage between three mouse X-linked genes has been demonstrated. The three genes, P3, G6pd and Cf-8 all lie within 400 Kb of DNA. This physical linkage mirrors the situation on the human X chromosome, representing the first demonstration of mouse/human synteny at the physical level. A detailed physical map encompassing 1.6 Mbp of this region is presented. A number of the rare cutter restriction enzyme sites within this map are partially blocked on the inactive X chromosome, presumably due to the methylation of CpG rich islands. Pulsed field gel electrophoresis therefore provides a useful tool for the study of X-inactivation over large regions of the X chromosome.
Brockdorff
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N
Clinical Research Centre, Comparative Biology Section, Harrow, Middlesex, UK.
Amar
L C
LC
Brown
S D
SD
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D005819:Genetic Markers / Q000032:analysis; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015183:Restriction Mapping; D013950:Thymus Gland; D014960:X Chromosome
D005819:Genetic Markers; D007202:Indicators and Reagents; D005954:Glucosephosphate Dehydrogenase