The S-100 protein in cerebrospinal fluid: a simple ELISA method.
A simple ELISA method is described for determinations of S-100 protein concentrations in CSF. The assay has a useful range of 200-3200 pmol/l. The precision of the ELISA was estimated using a pool of CSF. The coefficient of variation was 0.18 within assay, 0.17 between assay and 0.17 between day. The S-100 protein is stable in the CSF as no measurable differences in S-100 concentrations were observed in samples stored at room temperature for 2 days. No correlation between age and S-100 concentration was found when determinations were performed in CSF from neurologically healthy males. Furthermore, no changes of S-100 was observed in a lumbocisternal CSF gradient from patients with normal pressure hydrocephalus. Thus, the described ELISA represents an easy to handle and reliable method, well suited for routine determinations of S-100 protein concentration in the CSF.
Aurell
A
A
Institute of Neurobiology, University of Göteborg, Sweden.
Rosengren
L E
LE
Wikkelsø
C
C
Nordberg
G
G
Haglid
K G
KG
D000328:Adult; D000368:Aged; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009418:S100 Proteins / Q000134:cerebrospinal fluid*
D009418:S100 Proteins