title: Hemodynamic effects on endothelial cell monolayer detachment from vascular prostheses.
abstract: The establishment of an early blood-contacting endothelialized surface may improve the graft-host relationship. This study evaluated the adherence of indium 111-radiolabeled endothelial cells that were cultured to confluence on fibronectin-treated polyester elastomer (Hytrel) grafts that were perfused for two hours on a pulse duplicator apparatus under high- and low-shear conditions. Perfusate samples were serially assayed for radioactivity. After perfusion, grafts were sectioned into four segments and assayed for retained radioactivity. All graft segments were hematoxylin stained and examined under light microscopy for evaluation of cell density. Excellent endothelial cell adherence (90%) was observed under both hemodynamic conditions at 120 minutes, with most losses occurring within the first 15 minutes. No differences were seen between high- and low-shear conditions or proximal vs distal graft segments.
authors.affiliation: Department of Surgery, Loyola University Medical Center, Maywood Ill 60153.
authors.forename: H P
authors.initials: HP
authors.lastname: Greisler
authors.forename: E D
authors.initials: ED
authors.lastname: Endean
authors.forename: J J
authors.initials: JJ
authors.lastname: Klosak
authors.forename: J
authors.initials: J
authors.lastname: Ellinger
authors.forename: S C
authors.initials: SC
authors.lastname: Henderson
authors.forename: S M
authors.initials: SM
authors.lastname: Pham
authors.forename: S J
authors.initials: SJ
authors.lastname: Durham
authors.forename: D P
authors.initials: DP
authors.lastname: Showalter
authors.forename: J
authors.initials: J
authors.lastname: Levine
authors.forename: H S
authors.initials: HS
authors.lastname: Borovetz
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D002478:Cells, Cultured; D003470:Culture Media; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005353:Fibronectins; D006439:Hemodynamics; D007205:Indium Radioisotopes; D007601:Jugular Veins; D008954:Models, Biological; D011091:Polyesters; D012212:Rheology
chemical_list: D003470:Culture Media; D005353:Fibronectins; C059046:Hytrel; D007205:Indium Radioisotopes; D011091:Polyesters