Epithelial cell surfaces induce Salmonella proteins required for bacterial adherence and invasion.
Salmonella bacteria are capable of entering (invading) and multiplying within eukaryotic cells. Stable adherence to and invasion of epithelial cells by S. choleraesuis and S. typhimurium were found to require de novo synthesis of several new bacterial proteins. This inducible event appears to be a coordinately regulated system dependent on trypsin- and neuraminidase-sensitive structures present on the epithelial cell surface. Mutants of S. choleraesuis and S. typhimurium were unable to synthesize these proteins and did not stably adhere to nor invade eukaryotic cells. Two such S. typhimurium mutants were avirulent in mice, an indication that these proteins are required for Salmonella virulence.
Finlay
B B
BB
Department of Medical Microbiology, Stanford University School of Medicine, CA 94305.
Heffron
F
F
Falkow
S
S
D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002460:Cell Line; D004848:Epithelium / Q000502:physiology; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D012475:Salmonella / Q000472:pathogenicity / Q000502:physiology*; D013462:Sulfur Radioisotopes
D001426:Bacterial Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine