Sequence determination in oligosaccharides by relayed NOE experiments in the rotating frame.
An hybrid experiment, composed of 1H-NMR total correlation spectroscopy (TOCSY) and rotating frame nuclear Overhauser enhancement spectroscopy (ROESY) steps, makes it possible to determine both the partial (disaccharide) sequences, and the sequential order, of whole linear and branched oligosaccharide chains.
Poppe
L
L
Max-Planck-Institut für Medizinische Forschung, Heidelberg, Germany.
Dabrowski
J
J
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005577:Forssman Antigen; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides
D009844:Oligosaccharides; D005577:Forssman Antigen