[Detection of dipeptidyl peptidase IV in follicular fluid--initial report].
Aspirates of ovarian follicles were obtained from patients subjected to gynaecologic surgeries. This is the first report of dipeptidyl-peptidase IV (DP IV) activity in human follicular fluid. This enzyme specifically cleaves regulatory important oligo- and polypeptides. Only from peptides with proline or alanine in position P1 of the N-terminal sequence a dipeptide is split off. DP IV activity was measured with glycyl-L-proline p-Nitroanilide as substrate. Levels in a range of 209.8 +/- 57.4 nmol l-1 s-1 were achieved.
Seliger
E
E
Klinik und Poliklinik für Gynäkologie und Geburtshilfe, Martin-Luther-Universität Halle-Wittenberg.
Blumenauer
V
V
Heins
J
J
Neubert
K
K
Kaltwasser
P
P
Schöneich
C
C
Bergleiter
R
R
D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism*; D005260:Female; D006801:Humans; D006080:Ovarian Follicle / Q000201:enzymology*; D010455:Peptides / Q000378:metabolism
D010455:Peptides; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4
Der Nachweis der Dipeptidyl-Peptidase IV in der Follikelflüssigkeit--ein erster Bericht.