Rapid localization of carbon 14-labeled molecules in biological samples by ion mass microscopy.
We report here on the ability of secondary ion mass spectrometry (SIMS) to provide rapid imaging of the intracellular distribution of 14C-labeled molecules. The validity of this method, using mass discrimination of carbon 14 atoms, was assessed by imaging the distribution of two molecules of well-known metabolism, [14C]-thymidine and [14C]-uridine, incorporated by human fibroblasts in culture. As expected, 14C ion images showed the presence of [14C]-thymidine in the nucleus of dividing cells, whereas [14C]-uridine was present in the cytoplasm as well as the nucleus of all cells, with a large concentration in the nucleoli. The time required to obtain the distribution images with the SMI 300 microscope was less than 6 min, whereas microautoradiography, the classical method for mapping the tissue distribution of 14C-labeled molecules, usually requires exposure times of several months. Secondary ion mass spectrometry using in situ mass discrimination is proposed here as a very sensitive method which permits rapid imaging of the subcellular distribution of molecules labeled with carbon 14.
Hindie
E
E
Laboratoire de Biophysique, SC 27 de l'INSERM, Faculté de Médecine, Créteil, France.
Escaig
F
F
Coulomb
B
B
Lebreton
C
C
Galle
P
P
D002250:Carbon Radioisotopes / Q000032:analysis*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008853:Microscopy / Q000379:methods*
D002250:Carbon Radioisotopes