An in vitro radiolabel uptake viability assay for Onchocerca microfilariae.
A radiolabel uptake viability assay for Onchocerca cervicalis using [3H]2-deoxy-D-glucose in Hanks' balanced salt solution, pH 7.5, at 30 C is described and compared to the traditional visual motility assay. A correlation of r = 0.92 between the assays was found, with the radiolabel uptake method apparently a more sensitive indicator of microfilarial viability.
Callahan
H L
HL
Department of Ophthalmology, Medical University of South Carolina, Charleston 29425.
Wakeman
J M
JM
Crouch
R K
RK
James
E R
ER
D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008842:Microfilariae / Q000378:metabolism; D009068:Movement; D009854:Onchocerca / Q000378:metabolism*; D013696:Temperature; D014316:Tritium
D014316:Tritium; D003847:Deoxyglucose