Isolation, characterization, and synthesis of chrysobactin, a compound with siderophore activity from Erwinia chrysanthemi.
A catechcol-type siderophore, assigned the trivial name chrysobactin, was isolated from the phytopathogenic bacterium Erwinia chrysanthemi and characterized by degradation and spectroscopic techniques as N-[N2-(2,3-dihydroxybenzoyl)-D-lysyl]-L-serine. Chrysobactin, which was also obtained by chemical synthesis, was shown to be active in supplying iron to a group of mutants of E. chrysanthemi defective in biosynthesis of the siderophore.
Persmark
M
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Biochemistry Department, University of California, Berkeley 94720.
Expert
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D
Neilands
J B
JB
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004885:Erwinia / Q000032:analysis* / Q000235:genetics / Q000254:growth & development; D005029:Ethylenediamines; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009154:Mutation; D013053:Spectrophotometry
D000596:Amino Acids; D005029:Ethylenediamines; C011813:ethylenediamine-N,N'-bis(2-hydroxyphenylacetic acid); D007501:Iron