Tumor imaging of Dalton's lymphoma using antibody-desferal-Ga-67 complex.
Using desferal, a bifunctional chelating agent, Ga-67 was conjugated to the antibody, anti DLAA, specific for a 80 KD antigen associated with Dalton's lymphoma (DLAA) and was used for imaging of murine lymphomas induced by an 'air-pouch' technique. Scintiscan studies were carried out at 24 hr, 48 hr, 72 hr, after i.v. administration of Ga-67-anti DLAA-DF complex and compared with scintigrams obtained after administration of free Ga-67 citrate and suspension of Ga-67, DF, anti DLAA to different batches of tumor bearing mice. The study was also carried out using mouse IgG, a non-specific antibody, instead of anti DLAA. The results were compared with biodistribution studies. Scintigrams demonstrated that with anti DLAA there was maximum uptake of Ga-67 by the tumor providing the best images.
Udayachander
M
M
Biochemistry Department, Cancer Institute, Madras, India.
Meenakshi
A
A
Sivanandham
M
M
D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D002951:Citrates; D019343:Citric Acid; D003676:Deferoxamine; D007553:Isotope Labeling; D008223:Lymphoma / Q000000981:diagnostic imaging* / Q000276:immunology; D051379:Mice; D011877:Radionuclide Imaging
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D002951:Citrates; D019343:Citric Acid; D003676:Deferoxamine