Photochemical decontamination of blood components containing hepatitis B and non-A, non-B virus.
Diluted plasma samples containing 10(2), 10(3), 10(4), and 10(5) chimpanzee infectious doses (CID) of a human non-A, non-B hepatitis virus (NANBV) were treated with a combination of two psoralen compounds, 4'-aminomethyl-4,5',8-trimethylpsoralen and 4,5',8-trimethylpsoralen, and exposed to long wavelength ultraviolet. Each dilution was then transfused into one of four chimpanzees. In a second experiment, three samples containing 10(4.5) CID of hepatitis B virus (HBV) and two samples containing 10(4) CID of NANBV were treated with 8-methoxypsoralen (8-MOP) and ultraviolet irradiation. Two chimpanzees were each transfused with both a treated HBV and a treated NANBV sample. The third chimpanzee was inoculated with a treated HBV sample alone. In the six months after inoculation none of the animals showed biochemical or histological evidence of hepatitis. In experiments involving NANBV inocula, the susceptibility of the animals was confirmed by subsequent challenge with untreated NANBV. Factor VIII concentrate containing virus and photochemically treated as in the first experiment retained an average of 91% of its activity while that in the second experiment retained 94% of its activity.
Alter
H J
HJ
Department of Transfusion Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health Bethesda, MD 20205.
Creagan
R P
RP
Morel
P A
PA
Wiesehahn
G P
GP
Dorman
B P
BP
Corash
L
L
Smith
G C
GC
Popper
H
H
Eichberg
J W
JW
D000818:Animals; D001769:Blood / Q000382:microbiology*; D003666:Decontamination / Q000379:methods; D005169:Factor VIII / Q000032:analysis; D006515:Hepatitis B virus / Q000187:drug effects* / Q000528:radiation effects; D006518:Hepatitis Viruses / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D011701:PUVA Therapy; D002679:Pan troglodytes; D014307:Trioxsalen / Q000031:analogs & derivatives / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects / Q000528:radiation effects
D005169:Factor VIII; C018822:aminomethyltrioxsalen; D014307:Trioxsalen