title: Effect of plasma amino acid and hormone concentrations on renal plasma flow and glomerular filtration rate.
abstract: The effect of amino acid infusion on renal hemodynamics was examined in 13 healthy subjects. Seven subjects participated in the first experimental protocol: study 1 - amino acids were infused for 3 h to increase plasma amino acid levels 2- to 3-fold above baseline; study 2 - amino acids were infused with somatostatin and peripheral replacement of insulin, glucagon and growth hormone; and study 3 - somatostatin and hormones were infused as in study 2, but no amino acids were infused. During study 1 both glomerular filtration rate (GFR) and renal plasma flow (RPF) increased by 20%. In contrast, when amino acids were infused with somatostatin (study 2), neither GFR nor RPF changed from baseline. In study 3, somatostatin alone had no effect on GFR and RPF. In a second experimental protocol, 6 subjects were maintained for 7 days on a low protein diet (40 g/day); amino acids were infused as in study 1 before and after the protein restriction. Baseline GFR and RPF both decreased significantly after protein restriction. The response to amino acid infusion was not changed. These results indicate that: (1) hyperaminoacidemia causes a rise in both GFR and RPF; (2) the increase is likely to be mediated by some hormones whose secretion is inhibited by somatostatin, and (3) protein restriction causes a decline in GFR and RPF but fails to alter the renal hemodynamic response to amino acid infusion.
authors.affiliation: Department of Medicine, Yale University School of Medicine, New Haven, Conn.
authors.forename: P
authors.initials: P
authors.lastname: Castellino
authors.forename: R A
authors.initials: RA
authors.lastname: DeFronzo
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000097:blood* / Q000494:pharmacology; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D004347:Drug Interactions; D005919:Glomerular Filtration Rate / Q000187:drug effects*; D005934:Glucagon / Q000097:blood* / Q000494:pharmacology; D013006:Growth Hormone / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000097:blood* / Q000494:pharmacology; D012039:Regional Blood Flow / Q000187:drug effects; D012079:Renal Circulation / Q000187:drug effects*; D013004:Somatostatin / Q000097:blood* / Q000494:pharmacology; D013268:Stimulation, Chemical
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins; D007328:Insulin; D013004:Somatostatin; D013006:Growth Hormone; D005934:Glucagon