Structural similarity of chymopapain forms as indicated by circular dichroism.
Four chymopapain forms were isolated by high-resolution liquid chromatography on a cation-exchange column. The three major forms possess nearly identical secondary and tertiary structures, as judged from their c.d. spectra; these components showed similar proteolytic activity and Mr values close to that of papain. The fourth isolated component seems to be a mixture of modified proteins.
Solis-Mendiola
S
S
Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, México D.F., México.
Zubillaga-Luna
R
R
Rojo-Dominguez
A
A
Hernandez-Arana
A
A
D055598:Chemical Phenomena; D002621:Chemistry; D002917:Chymopapain; D002942:Circular Dichroism; D008970:Molecular Weight
D002917:Chymopapain