title: Cariogenicity of a lactate dehydrogenase-deficient mutant of Streptococcus mutans serotype c in gnotobiotic rats.
abstract: A lactate dehydrogenase-deficient (Ldh-) mutant of a human isolate of Streptococcus mutans serotype c was tested in a gnotobiotic rat caries model. Compared with the wild-type Ldh-positive (Ldh+) strains, it was significantly (alpha less than or equal to 0.005) less cariogenic in experiments with two different sublines of Sprague-Dawley rats. The Ldh- mutant strain 044 colonized the oral cavity of the test animals to the same extent as its parent strain 041, although its initial implantation was slightly but not significantly (P greater than or equal to 0.2) less. Multiple oral or fecal samples plated on 2,3,5-triphenyltetrazolium indicator medium revealed no evidence of back mutation from Ldh- to Ldh+ in vivo. Both Ldh+ strain 041 and Ldh- strain 044 demonstrated bacteriocinlike activity in vitro against a number of human strains of mutans streptococci representing serotype a (S. cricetus) and serotypes c and e (S. mutans). Serotypes b (S. rattus) and f (S. mutans) and strains of S. mitior, S. sanguis, and S. salivarius were not inhibited. Thus, Ldh mutant strain 044 possesses a number of desirable traits that suggest it should be investigated further as a possible effector strain for replacement therapy of dental caries. These traits include its stability and low cariogenicity in the sensitive gnotobiotic rat caries model, its bacteriocinlike activity against certain other cariogenic S. mutans (but not against more inocuous indigenous oral streptococci), and the fact that it is a member of the most prevalent human serotype of cariogenic streptococci.
authors.affiliation: Research Service, Veterans Administration Medical Center, Miami, Florida 33125.
authors.forename: R J
authors.initials: RJ
authors.lastname: Fitzgerald
authors.forename: B O
authors.initials: BO
authors.lastname: Adams
authors.forename: H J
authors.initials: HJ
authors.lastname: Sandham
authors.forename: S
authors.initials: S
authors.lastname: Abhyankar
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis; D001835:Body Weight; D003731:Dental Caries / Q000382:microbiology*; D005856:Germ-Free Life; D007770:L-Lactate Dehydrogenase / Q000172:deficiency / Q000502:physiology*; D009055:Mouth / Q000382:microbiology; D051381:Rats; D011919:Rats, Inbred Strains; D013295:Streptococcus mutans / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*
chemical_list: D001430:Bacteriocins; D007770:L-Lactate Dehydrogenase