title: The primary structure of human apolipoprotein A-IV.
abstract: Human apolipoprotein (apo) A-IV was purified from chylous ascites fluid. Proteolytic peptides produced by trypsin and Staphylococcus aureus V8 proteinase digestions were purified by high-performance liquid chromatography and sequenced. Human apoA-IV contains 376 amino acid residues. The peptide-derived sequence generally matches two previously reported DNA-derived amino acid sequences except for discrepancies in five positions. In order to examine these discrepancies further, one complete apoA-IV cDNA clone and another partial clone were sequenced. Comparison of all the available information indicates that the peptide-derived sequence reported here is accurate. Sequencing errors probably account for some of the discrepancies between the two primary sequences predicted by earlier nucleotide analyses. In certain positions, however, bona fide sequence heterogeneity or cloning artifact cannot be excluded.
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, TX 77030.
authors.forename: C Y
authors.initials: CY
authors.lastname: Yang
authors.forename: Z W
authors.initials: ZW
authors.lastname: Gu
authors.forename: I S
authors.initials: IS
authors.lastname: Chong
authors.forename: W J
authors.initials: WJ
authors.lastname: Xiong
authors.forename: M
authors.initials: M
authors.lastname: Rosseneu
authors.forename: H X
authors.initials: HX
authors.lastname: Yang
authors.forename: B R
authors.initials: BR
authors.lastname: Lee
authors.forename: A M
authors.initials: AM
authors.lastname: Gotto
authors.forename: L
authors.initials: L
authors.lastname: Chan
mesh_terms: D000595:Amino Acid Sequence; D001054:Apolipoproteins A / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D012697:Serine Endopeptidases; D014357:Trypsin
chemical_list: D001054:Apolipoproteins A; D003062:Codon; D010446:Peptide Fragments; C022205:apolipoprotein A-IV; D004247:DNA; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin