Cell cycle dependent distribution of the proliferation-associated Ki-67 antigen in human embryonic lung cells.
The cell cycle-dependent distribution of the proliferation-associated Ki-67 antigen has been evaluated immunocytochemically in L-132 human fetal lung cells. The cells were synchronized and cell cycle phases were determined: G1 = 6.7 h, S = 5.4 h, G2 = 8.5 h and mitosis = 1.3 h. The Ki-67 patterns were strictly correlated with the cell cycle phases. In late G1-phase, Ki-67 antigen was present only in the perinucleolar region. In the S-phase, Ki-67 staining was found homogeneously in the karyoplasm and in the perinucleolar region. G2-phase cells contained a finely granular Ki-67 staining in the karyoplasm with Ki-67-positive specks and perinucleolar staining. In early mitotic cells (pro- and metaphase) an intense perichromosomal Ki-67 staining was observed in addition to a homogeneously stained karyoplasm in prophase, and cytoplasm in metaphase. During ana- and telophase the Ki-67 antigen disappeared rapidly. In resting cells there was no Ki-67 staining.
Braun
N
N
Institute of Pathology, Julius-Maximilians Universität, Würzburg, Federal Republic of Germany.
Papadopoulos
T
T
Müller-Hermelink
H K
HK
D000941:Antigens / Q000276:immunology*; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D004622:Embryo, Mammalian; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung
D000941:Antigens