Isolation of calelectrin-like proteins associated with smooth muscle plasma membranes.
Plasma membranes prepared from pig stomach smooth muscle (antral part) were extracted with Triton X-100 to isolate insoluble cytoskeletal components. Reextraction of the insoluble material in EGTA yielded a protein complex which resembled the family of proteins that has been designated as 'mammalian calelectrins' [Südhof, T.C. et al. (1984) Biochemistry 23, 1103-1109]. Plasma membranes prepared in the presence and in the absence of 0.6 M KCl differed by the amount of these proteins, but in both preparations the EGTA-extractable proteins were quantitatively important constituents. Two of these proteins were further purified by means of ion exchange chromatography to apparent homogeneity as judged from sodium dodecyl sulfate gel electrophoresis.
Raeymaekers
L
L
Wuytack
F
F
Casteels
R
R
D000818:Animals; D017302:Annexins; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis*; D003598:Cytoskeletal Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000032:analysis*; D017830:Octoxynol; D011092:Polyethylene Glycols; D011706:Pyloric Antrum / Q000032:analysis; D013552:Swine
D017302:Annexins; D002135:Calcium-Binding Proteins; D003598:Cytoskeletal Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; D002118:Calcium