title: Modulation of alcohol dehydrogenase isoenzyme levels in Zymomonas mobilis by iron and zinc.
abstract: Zymomonas mobilis is an unusual microorganism which utilizes both iron-containing alcohol dehydrogenase (ADHII) and zinc-containing alcohol dehydrogenase (ADHI) isoenzymes during fermentative growth. This organism is obligately ethanologenic, and alcohol dehydrogenase activity is essential. The activities of ADHI and ADHII were altered by supplementing growth medium with iron or zinc salts and by iron starvation. Growth under iron-limiting conditions (chelators, minimal medium) reduced ADHII activity but did not prevent the synthesis of the ADHII protein. The inactive form of this enzyme appeared quite stable, was not renatured by iron addition, and persisted in the cell. The iron-induced increase in ADHII activity required de novo synthesis which was blocked by antibiotic additions. The ability of Z. mobilis to synthesize ADHII and ADHI may be advantageous in nature.
authors.affiliation: Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
authors.forename: K F
authors.initials: KF
authors.lastname: Mackenzie
authors.forename: C K
authors.initials: CK
authors.lastname: Eddy
authors.forename: L O
authors.initials: LO
authors.lastname: Ingram
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D005296:Ferrous Compounds / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D013431:Sulfates / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology*; D019287:Zinc Sulfate
chemical_list: D005296:Ferrous Compounds; D007527:Isoenzymes; D013431:Sulfates; C020748:ferrous sulfate; D019287:Zinc Sulfate; D000426:Alcohol Dehydrogenase; D015032:Zinc