Is there any correlation between platelet aggregation, plasma lipoproteins, apoproteins and membrane fluidity of human blood platelets?
Fluorescence anisotropy (which is inversely related to membrane fluidity) of gel filtered platelets of 18 normolipemic subjects (20-26 years) was measured after incubation with three different fluorescent probes (DPH, TMA-DPH, and 6-As). These values were correlated to both platelet aggregation parameters after stimulation with ADP (4 microM), epinephrine (10 microM) or collagen (2 micrograms/ml PRP) and to plasma lipids, lipoproteins and apoproteins. Fluorescence anisotropy values after DPH-labeling of platelets were only negatively correlated to TG (p less than 0.05). No correlation was found between fluorescence anisotropy values of DPH, TMA-DPH and 6-As to LDL-C, Lp(a), HDL-C and HDL3-C (p less than 0.01). However, fluorescence anisotropy values of DPH and TMA-DPH were negatively correlated to apoproteins A2 and B (p less than 0.05). No correlations were found between fluorescence anisotropy after DPH labeling and different aggregation parameters. TMA-DPH and 6-As fluorescence anisotropy values are correlated to epinephrine induced stimulation (p less than 0.01).
Malle
E
E
Institute of Medical Biochemistry, University of Graz, Austria.
Gries
A
A
Kostner
G M
GM
Pfeiffer
K
K
Nimpf
J
J
Hermetter
A
A
D000328:Adult; D001059:Apoproteins / Q000097:blood*; D005454:Fluorescence Polarization; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008560:Membrane Fluidity; D010974:Platelet Aggregation
D001059:Apoproteins; D008074:Lipoproteins