DNA bending and binding factors of the human beta-actin promoter.
Transcription of the beta-actin gene is rapidly inducible in response to serum stimulation. To determine the regions responsible for serum inducible and basal level expression, the human beta-actin promoter was subjected to mutational analysis. Two distinct elements, the CCAAT homology and the beta-actin specific conserved sequences, were found by a chloramphenicol acetyltransferase expression assay and sequence comparisons, and then analyzed for possible functions. Using a DNA bend assay, it was shown that the conserved sequences included the core of a sequence-directed bend of DNA. Gel mobility shift and DNase I protection assays revealed that the conserved sequences and the CCAAT homology were recognized by binding factors in HeLa cell extracts.
Kawamoto
T
T
Department of Oncogene Research, Osaka University, Japan.
Makino
K
K
Orita
S
S
Nakata
A
A
Kakunaga
T
T
D000199:Actins / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002645:Chickens; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D000199:Actins; D004268:DNA-Binding Proteins