title: The effect of eye-derived-growth-factor (EDGFs) on methionine incorporation in the different cell populations of bovine adult lens in organ culture.
abstract: When adult bovine lenses were cultured in vitro, the purified retina-derived growth factors EDGF I or EDGF II, as well as the soluble fraction of the retina RE, increased the rate of incorporation of [35S]methionine into protein in cells belonging to different populations in the anterior epithelium as well as in fibers from the most superficial region of the cortex. These fiber cells were the most sensitive to stimulation by the retinal factors as they exhibit a significant increase of total protein synthesis 24 hr after addition of the factors to the culture medium. The epithelial cells studied--central epithelial cells and germinative cells--appeared stimulated only 1 day later. The stimulation of incorporation was not directed towards a particular subset of proteins but to all major polypeptides constituting the electrophoretic pattern of each cell population. It is suggested that this type of ocular signal, which stimulates the expression of a definite program, may act as a permissive signal.
authors.affiliation: Unité de Recherches Gérontologiques, U. 118 INSERM, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Mascarelli
authors.forename: Y
authors.initials: Y
authors.lastname: Courtois
authors.forename: C
authors.initials: C
authors.lastname: Arruti
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D003459:Crystallins / Q000096:biosynthesis; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D009799:Ocular Physiological Phenomena; D009924:Organ Culture Techniques; D010452:Peptide Biosynthesis; D013268:Stimulation, Chemical
chemical_list: D003459:Crystallins; D006133:Growth Substances; C039825:eye-derived growth factor; D005346:Fibroblast Growth Factors; D008715:Methionine