Anti-IgM antibodies inhibit IgM expression in lipopolysaccharide-stimulated normal murine B-cells: study of RNA metabolism and translation.
Activation of resting mouse B-cells with anti-immunoglobulin M (IgM) antibodies and a mitogen such as bacterial lipopolysaccharide leads to a specific inhibition of IgM expression. [3H]Uridine pulse-chase experiments show that the inhibition of RNA metabolism by anti-IgM antibody treatment occurs in the nucleus at the mu-RNA processing level and results in low levels of mature mu-mRNA expression. By applying the RNA synthesis inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole, the half lives of various forms of Ig-mRNA are estimated to be 8-12 h. The fact that the residual mu-mRNA left in B-cells after anti-IgM antibody treatment can still be translated into polypeptides indicates that the mu-mRNA in these cells is still functional.
Chen
U
U
Basel Institute for Immunology, Switzerland.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D005803:Genes, Immunoglobulin; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics*; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012016:Reference Values; D014158:Transcription, Genetic
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D012333:RNA, Messenger; D011061:Poly A