Reversal by interferon-gamma of inhibition of delayed-type hypersensitivity induction by anti-CD4 or anti-interleukin 2 receptor (CD25) monoclonal antibodies. Evidence for the physiological role of the CD4+ TH1+ subset in mice.
In mice, induction of delayed-type hypersensitivity (DTH) and of antibody formation to sheep red blood cells (SRBC) was inhibited by the anti-CD4 monoclonal antibody (mAb) GK1.5. However, anti-interleukin 2 mAb AMT-13 inhibited DTH response but not antibody formation. The inhibitory action of the mAb on DTH, but not an antibody formation, was reversed by concomitant injection of recombinant mouse interferon-gamma. These data provide the first in vivo evidence for the postulated physiological role of the CD4+ TH1 lymphocyte subset and indicate that the action of anti-interleukin 2 receptor mAb is restricted to this T helper subset.
Diamantstein
T
T
Institut für Immunologie, Klinikum Steglitz, Freie Universität Berlin.
Eckert
R
R
Volk
H D
HD
Kupier-Weglinski
J W
JW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology; D051379:Mice; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D007371:Interferon-gamma