title: Nuclear DNA synthesis is blocked by UV irradiation in Dictyostelium discoideum.
abstract: We have used a thymidine auxotroph of the simple eukaryote, Dictyostelium discoideum and alkaline sucrose gradients of isolated nuclei to study alterations in DNA synthesis following irradiation of replicating haploid cells with 254 nm UV light. Three responses were characterized using pulse-chase protocols: (1) Lags in DNA synthesis as measured by the amount of label incorporated were 4, 9, and 20 h after 10, 50, and 200 J/m2. (2) The DNA synthesized during a 15-min pulse immediately after irradiation was of lower single strand molecular weight: 7, 3.5, and 3 x 10(6) dalton after 0, 50, and 200 J/m2. (3) The time required for maturation of the nascent DNA to full-sized single strands of about 2 x 10(8) dalton was 45-50 min for unirradiated cells, 3 h after 10 J/m2, and 20 h after 200 J/m2. The DNA of the irradiated cells did not mature uniformly during these delays; instead, a period of no increase in size was followed by a rapid, nearly control rate of maturation. We conclude: (a) at least some UV lesions block elongation of replicons; (b) the elongation of the replicons and their subsequent joining to yield mature high molecular weight DNA occurs after most of the lesions are repaired; (c) the timing of the different aspects of recovery suggest that initiation of replication is also inhibited.
authors.affiliation: Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
authors.forename: D L
authors.initials: DL
authors.lastname: Hurley
authors.forename: A M
authors.initials: AM
authors.lastname: Skantar
authors.forename: R A
authors.initials: RA
authors.lastname: Deering
authors.forename: A M
authors.initials: AM
authors.lastname: Skantarz
mesh_terms: D002467:Cell Nucleus / Q000648:ultrastructure; D004260:DNA Repair; D004261:DNA Replication; D004023:Dictyostelium / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays