Alanine:glyoxylate aminotransferase activities in liver of Suncus murinus (insectivora).
1. The distribution of L-alanine:glyoxylate aminotransferase (AGT) activities were found in Suncus liver, 55% in particulate fraction and 45% in supernatant. 2. 65% of AGT activities in particulate were dependent on AGT isoenzyme 2 (AGT 2) having molecular weight 210,000, the remainder (35%) of AGT activities were dependent on AGT isoenzyme 1 (AGT 1) which have aminotransferase activity for serine. AGT activities in supernatant were dependent on AGT 1, AGT 2 and alanine:2-oxoglutarate aminotransferase (GPT), and their activity ratios were 10, 15 and 75%, respectively. 3. Km values for alanine were 0.52 mM; AGT 1, 3.3 mM; AGT 2, 0.88 mM; GPT measuring with AGT activity. AGT activity of GPT was inhibited by addition of glutamate and its Ki value was 1.8 mM. 4. Some other properties of AGT 1, AGT 2 and GPT are described.
Okuno
E
E
Department of Biochemistry, Wakayama Medical College, Japan.
Ishikawa
T
T
Kawai
J
J
Kido
R
R
D000410:Alanine Transaminase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D007312:Eulipotyphla / Q000378:metabolism*; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007526:Isoelectric Point; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity; D000637:Transaminases
D005971:Glutamates; D007527:Isoenzymes; D018698:Glutamic Acid; D000637:Transaminases; D000410:Alanine Transaminase; C019635:Alanine-glyoxylate transaminase