Cell-free human amniotic fluid as culture medium for mouse and human embryos.
Differences between murine and human preimplantation embryonic growth in cell-free human amniotic fluid and medium F-10 supplemented with human fetal cord serum were assessed. Two-cell mouse embryos cultured in human amniotic fluid developed more consistently to the expanded blastocyst stage than those cultured in F-10. No significant difference in human preimplantation embryo cleavage was observed between the two culture conditions. However, amniotic fluid was significantly worse than F-10 + human serum in its ability to maintain embryos that could establish pregnancy after transfer.
Dorfmann
A D
AD
Genetics & IVF Institute, Fairfax, Virginia 22031.
Bender
S D
SD
Robinson
P
P
Fugger
E F
EF
Bustillo
M
M
Reed
G
G
Schulman
J D
JD
D000653:Amniotic Fluid / Q000502:physiology*; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D003470:Culture Media; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009924:Organ Culture Techniques
D003470:Culture Media