title: Passive protection of rabbits infected with toxic shock syndrome-associated strains of Staphylococcus aureus by monoclonal antibody to toxic shock syndrome toxin 1.
abstract: The effectiveness of passive immunization was assessed in an infection model of toxic shock syndrome (TSS) in which monoclonal antibody to TSS toxin 1 (TSST-1) was administered intravenously to rabbits. Previously implanted infection chambers were inoculated with Staphylococcus aureus strains RN4710 and D4508. The former strain carries the TSST-1 gene on plasmid pRN6201; the latter is a TSST-1-negative clinical isolate obtained from a patient with nonmenstrual TSS. Purified monoclonal antibody, MAb 8-5-7 (IgG), was administered in two doses of approximately 1.25 mg each 24 hours before and 24 hours after infection. MAb 8-5-7 provided complete protection against both the TSS-like syndrome and the mortality that occurred in unprotected rabbits infected with strain RN4710 but did not provide complete protection in rabbits infected with strain D4508; three of the five rabbits either displayed signs of illness or died despite treatment. Western-blot analyses of the extracellular proteins produced by strains RN4710 and D4508 that used MAb 8-5-7 as a probe revealed that only TSST-1 produced by RN4710 reacted with the antibody. Thus, if MAb 8-5-7 partially protected animals against infections with strain D4508, the protection appears to have been nonspecific.
authors.affiliation: Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912.
authors.forename: D F
authors.initials: DF
authors.lastname: Scott
authors.forename: G K
authors.initials: GK
authors.lastname: Best
authors.forename: J M
authors.initials: JM
authors.lastname: Kling
authors.forename: M R
authors.initials: MR
authors.lastname: Thompson
authors.forename: L E
authors.initials: LE
authors.lastname: Adinolfi
authors.forename: P F
authors.initials: PF
authors.lastname: Bonventre
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001427:Bacterial Toxins; D015153:Blotting, Western; D004768:Enterotoxins / Q000276:immunology*; D007116:Immunization, Passive; D008297:Male; D010957:Plasmids; D011817:Rabbits; D012772:Shock, Septic / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology*; D018089:Superantigens
chemical_list: D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal