Specific recognition of cruciform DNA by nuclear protein HMG1.
Cruciform DNA, a non-double helix form of DNA, can be generated as an intermediate in genetic recombination as well as from palindromic sequences under the effect of supercoiling. Eukaryotic cells are equipped with a DNA-binding protein that selectively recognizes cruciform DNA. Biochemical and immunological data showed that this protein is HMG1, an evolutionarily conserved, essential, and abundant component of the nucleus. The interaction with a ubiquitous protein points to a critical role for cruciform DNA conformations.
Bianchi
M E
ME
European Molecular Biology Laboratory, Heidleberg, Federal Republic of Germany.
Beltrame
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M
Paonessa
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G
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006609:High Mobility Group Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007118:Immunoassay; D015151:Immunoblotting; D008099:Liver / Q000032:analysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D010446:Peptide Fragments / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis; D051381:Rats; D014158:Transcription, Genetic
D006609:High Mobility Group Proteins; D010446:Peptide Fragments; D004247:DNA