title: The biotransformed metabolite profiles in blood after intravenous administration of dehydrocholic acid.
abstract: One gram of dehydrocholic acid was injected intravenously into two patients with percutaneous transhepatic cholangial drainage, and the biotransformed metabolites profiles over a 120-min period in serum and bile were analyzed. In serum unconjugated 3 alpha-hydroxy-7,12-dioxo-cholanoic acid (3 alpha-OH-7,12-OXO) acid was markedly increased after the injection of dehydrocholic acid, and reached about 80 microM within 30 min. On the other hand, conjugated 3 alpha, 7 alpha-dihydroxy-12-oxo-cholanoic acid (3 alpha,7 alpha-OH-12-OXO) was consecutively increased with lag time to an elevation of 3 alpha-OH-7,12-OXO. In bile, the major constituent of metabolites was conjugated 3 alpha,7 alpha-OH-12-OXO (more than 90% of the excreted metabolites), while conjugated 3 alpha-OH-7,12-OXO was detected as minor constituent. Therefore, between bile and serum, there was great difference in biotransformed metabolites profiles. The mechanism of elevation of unconjugated 3 alpha-OH-7,12-OXO in serum remains obscure, but this new finding raised a question as to whether organ-reducing 3-keto group of dehydrocholic acid is restricted to the liver.
authors.affiliation: Third Department of Internal Medicine, Hirosaki University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yoneda
authors.forename: I
authors.initials: I
authors.lastname: Makino
authors.forename: N
authors.initials: N
authors.lastname: Tamasawa
authors.forename: K
authors.initials: K
authors.lastname: Takebe
authors.forename: K
authors.initials: K
authors.lastname: Sakuraba
authors.forename: J
authors.initials: J
authors.lastname: Goto
authors.forename: T
authors.initials: T
authors.lastname: Nambara
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001646:Bile / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000032:analysis / Q000097:blood*; D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D003138:Common Bile Duct Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D003685:Dehydrocholic Acid / Q000008:administration & dosage* / Q000097:blood; D004322:Drainage; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D008875:Middle Aged
chemical_list: D001647:Bile Acids and Salts; D002635:Chenodeoxycholic Acid; C025576:3,7-dihydroxy-12-oxocholanoic acid; D003685:Dehydrocholic Acid