DNA restriction fragment length polymorphisms at either end of the c-raf-1 locus at 3p25.
We report the identification and characterization of two restriction fragment polymorphisms (RFLPs) associated with the human c-raf-1 protooncogene located on chromosome 3 (3p25). EcoRI digestion produces an RFLP derived from the 5'flanking sequences with a heterozygosity of 28%, and TaqI produces an RFLP representing the 3' flanking sequences with a heterozygosity of 25%. The finding of RFLPs associated with a potential oncogene may provide a method of analysis for determining genetic susceptibilities to certain cancers.
Graziano
S L
SL
Department of Medicine, SUNY-Health Science Center, Syracuse 13210.
Mark
G E
GE
Murray
C
C
Mann
D L
DL
Ehrlich
G D
GD
Poiesz
B J
BJ
Weston
A
A
D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D006801:Humans; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf
D011518:Proto-Oncogene Proteins; D019908:Proto-Oncogene Proteins c-raf