Correlation between intracellular pH and lactate levels in the rat brain during potassium cyanide induced metabolism blockade: a combined 31P-1H in vivo nuclear magnetic spectroscopy study.
Female Sprague-Dawley rats were chronically implanted with nuclear magnetic resonance (NMR) surface coils tuned to both 31P and 1H NMR frequencies. Alternated 31P and 1H NMR spectra were recorded at 2.5 min intervals in waking rats or rats under pentobarbital or chloral hydrate anesthesia. After a reference period, the metabolic changes were observed following intraperitoneal injection of potassium cyanide (KCN, 5 mg/kg). Among previously observed changes typical of cellular anoxia, attention was specifically paid to the relationship between the intracellular pH values and the lactate levels. The results show a strong lactate-pH correlation in waking rats, a partial decoupling under nembutal anesthesia and a complete decoupling under chloral hydrate anesthesia.
Lotito
S
S
Département de Biophysique, Faculté de Médecine, Université Joseph Fourier de Grenoble, la Tronche, France.
Blondet
P
P
François
A
A
von Kienlin
M
M
Rémy
C
C
Albrand
J P
JP
Décorps
M
M
Benabid
A L
AL
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology*; D005260:Female; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D010758:Phosphorus; D011190:Potassium Cyanide / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D003486:Cyanides; D007773:Lactates; D010758:Phosphorus; D019344:Lactic Acid; D006859:Hydrogen; D011190:Potassium Cyanide