title: Histamine is an intracellular messenger mediating platelet aggregation.
abstract: Inhibition of human platelet aggregation by N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine-HCl (DPPE), a novel antagonist of histamine binding, suggested that histamine might serve a critical role in cell function. Phorbol-12-myristate-13-acetate (PMA) or collagen was found to increase platelet histamine content in parallel with promotion of aggregation. Inhibitors of histidine decarboxylase (HDC) suppressed both aggregation and the elevation of histamine content, whereas DPPE inhibited aggregation only. In saponin-permeabilized platelets, added histamine reversed the inhibition by DPPE or HDC inhibitors on aggregation induced by PMA or collagen. The results indicate a role for histamine as an intracellular messenger, which in platelets promotes aggregation.
authors.affiliation: Department of Pediatrics, University of Manitoba, Winnipeg, Canada.
authors.forename: S P
authors.initials: SP
authors.lastname: Saxena
authors.forename: L J
authors.initials: LJ
authors.lastname: Brandes
authors.forename: A B
authors.initials: AB
authors.lastname: Becker
authors.forename: K J
authors.initials: KJ
authors.lastname: Simons
authors.forename: F S
authors.initials: FS
authors.lastname: LaBella
authors.forename: J M
authors.initials: JM
authors.lastname: Gerrard
mesh_terms: D001792:Blood Platelets / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000494:pharmacology; D003593:Cytoplasm / Q000502:physiology; D006632:Histamine / Q000502:physiology*; D006640:Histidine Decarboxylase / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D006632:Histamine; D003094:Collagen; D006640:Histidine Decarboxylase; D013755:Tetradecanoylphorbol Acetate