title: A new heparin-inhibited and polyamine-activated protein kinase from bovine kidney.
abstract: Two casein kinases, casein kinase-1 (CK-1) and casein kinase-2 (CK-2), have been characterized from many sources. In this study we describe the properties of a third casein kinase, designated casein kinase-3 (CK-3). CK-3 (Mr 32,000) is readily separated from CK-2 by gel filtration and from CK-1 by hydroxyapatite chromatography. CK-3 phosphorylates several proteins, including phosphorylase kinase. Phosphorylation of phosphorylase kinase by CK-3 results in a 10-fold enzyme activation. CK-3 is activated by spermine and inhibited by heparin, ADP, and divalent metal ions (Mn2+, Zn2+). Heparin inhibition of the kinase is reversed by spermine. The physical and regulatory properties of CK-3 are very similar to CK-1, suggesting that these kinases may be closely related.
authors.affiliation: Department of Biology, University of Waterloo, Ontario, Canada.
authors.forename: T J
authors.initials: TJ
authors.lastname: Singh
mesh_terms: D000818:Animals; D047388:Casein Kinases; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology*; D011073:Polyamines / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D013096:Spermine / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D011073:Polyamines; D047428:Protein Kinase Inhibitors; D013096:Spermine; D006493:Heparin; D011494:Protein Kinases; D047388:Casein Kinases