title: High resolution chromosomes from first trimester trophoblast cultures.
abstract: A simple method for obtaining prometaphase chromosomes from cultured first trimester cells involves the addition of BrdU and FdU 11 h before harvest and ethidium bromide 1.5 h before harvest. High resolution R-banding is obtained by acridine orange staining.
authors.forename: F
authors.initials: F
authors.lastname: Søndergaard
authors.forename: M
authors.initials: M
authors.lastname: Kristensen
authors.forename: N
authors.initials: N
authors.lastname: Tommerup
mesh_terms: D001973:Bromodeoxyuridine; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D004996:Ethidium; D005260:Female; D005467:Floxuridine; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011296:Prenatal Diagnosis / Q000379:methods*; D014327:Trophoblasts / Q000648:ultrastructure*
chemical_list: D005467:Floxuridine; D004996:Ethidium; D001973:Bromodeoxyuridine