The effect of an uptake inhibitor (dihydrokainate) on endogenous excitatory amino acids in the lamprey spinal cord as revealed by microdialysis.
Microdialysis was utilized to test the effects of the uptake inhibitor dihydrokainate (DHK) on endogenous amino acid levels in the lamprey spinal cord in vitro. The level of L-glutamate increased markedly (165%) in the presence of DHK, whereas the level of the glutamate precursor L-glutamine decreased (53%). Since DHK can potentiate or evoke fictive locomotion in the lamprey spinal cord, it is suggested that L-glutamate is released by neurons which take part in the activation of the spinal locomotor network.
Brodin
L
L
Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden.
Tossman
U
U
Ohta
Y
Y
Ungerstedt
U
U
Grillner
S
S
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism*; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D007798:Lampreys; D018377:Neurotransmitter Agents / Q000378:metabolism*; D012016:Reference Values; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005971:Glutamates; D018377:Neurotransmitter Agents; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; C020889:dihydrokainic acid; D005680:gamma-Aminobutyric Acid; D007608:Kainic Acid