title: Tissue activities of enzymes of diagnostic interest in the marmoset and rat.
abstract: The activities of enzymes of diagnostic interest were investigated in the liver, heart, kidney and muscle of the marmoset (Callithrix jacchus) and the rat. Methods of tissue extraction which gave maximal enzyme activity were used and comparison between the species showed some major differences. AST, LDH and GDH showed a similar distribution in both species but ICDH activity was much higher in the rat heart than in any other rat or marmoset organ. ALP, LAP and GGT were present in much higher activities in the rat kidney than in the marmoset kidney, a finding which was reversed in the liver of these animals. The major ALT-containing organ in the rat was the liver but, in the marmoset, this enzyme was found in relatively large quantities in the heart and muscle also. These differences can be of importance when plasma enzyme activities are measured following tissue damage.
authors.affiliation: Drug Safety Laboratory, Roche Products Limited, Welwyn Garden City, U.K.
authors.forename: C W
authors.initials: CW
authors.lastname: Davy
authors.forename: A
authors.initials: A
authors.lastname: Brock
authors.forename: J M
authors.initials: JM
authors.lastname: Walker
authors.forename: D A
authors.initials: DA
authors.lastname: Eichler
mesh_terms: D000410:Alanine Transaminase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D002143:Callitrichinae / Q000378:metabolism*; D004796:Clinical Enzyme Tests; D005260:Female; D005969:Glutamate Dehydrogenase / Q000032:analysis; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007668:Kidney / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D051381:Rats / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000032:analysis
chemical_list: D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005969:Glutamate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D007931:Leucyl Aminopeptidase