Voltage-dependent introduction of a d[alpha]octothymidylate into electropermeabilized cells.
DC-3F cells were submitted to electric square wave pulses in the presence of a d[alpha]octothymidylate 32P labelled in the 5' position. Radioactivity was incorporated in a voltage-dependent manner and reached a maximum for a field intensity of 1300-1400 V.cm-1. Growth curves and parallel cloning efficiency experiments indicated that cell viability was not altered by electric pulses, alone or in the presence of the oligothymidylate, below a field intensity of 1300 V.cm-1. Using affinity chromatography we extracted the incorporated oligonucleotide and showed that it was not degraded during the electropermeabilization experiment time.
Bazile
D
D
INSERM U 140, Institut Gustave Roussy, Villejuif, France.
Mir
L M
LM
Paoletti
C
C
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects / Q000502:physiology; D006224:Cricetinae; D003412:Cricetulus; D004558:Electric Stimulation; D004560:Electricity; D008168:Lung; D013938:Thymidine Monophosphate / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D013942:Thymine Nucleotides / Q000378:metabolism*
D013942:Thymine Nucleotides; C058833:3'-thymidylic acid; D013938:Thymidine Monophosphate