title: Toxicity and mutagenicity of plumbagin and the induction of a possible new DNA repair pathway in Escherichia coli.
abstract: Actively growing Escherichia coli cells exposed to plumbagin, a redox cycling quinone that increases the flux of O2- radicals in the cell, were mutagenized or killed by this treatment. The toxicity of plumbagin was not found to be mediated by membrane damage. Cells pretreated with plumbagin could partially reactivate lambda phage damaged by exposure to riboflavin plus light, a treatment that produces active oxygen species. The result suggested the induction of a DNA repair response. Lambda phage damaged by H2O2 treatment were not reactivated in plumbagin-pretreated cells, nor did H2O2-pretreated cells reactivate lambda damaged by treatment with riboflavin plus light. Plumbagin treatment did not induce lambda phage in a lysogen, nor did it cause an increase in beta-galactosidase production in a dinD::Mu d(lac Ap) promoter fusion strain. Cells pretreated with nonlethal doses of plumbagin showed enhanced survival upon exposure to high concentrations of plumbagin, but were unchanged in their susceptibility to far-UV irradiation. polA and recA mutants were not significantly more sensitive than wild type to killing by plumbagin. However, xth-1 mutants were partially resistant to plumbagin toxicity. It is proposed that E. coli has an inducible DNA repair response specific for the type of oxidative damage generated during incubation with plumbagin. Furthermore, this response appears to be qualitatively distinct from the SOS response and the repair response induced by H2O2.
authors.forename: S B
authors.initials: SB
authors.lastname: Farr
authors.forename: D O
authors.initials: DO
authors.lastname: Natvig
authors.forename: T
authors.initials: T
authors.lastname: Kogoma
mesh_terms: D010582:Bacteriophage lambda; D004260:DNA Repair / Q000187:drug effects*; D004926:Escherichia coli / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008027:Light; D008242:Lysogeny / Q000187:drug effects; D009152:Mutagenicity Tests; D009285:Naphthoquinones / Q000633:toxicity*; D009876:Operon; D012256:Riboflavin / Q000633:toxicity; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D009285:Naphthoquinones; D006861:Hydrogen Peroxide; D001616:beta-Galactosidase; D012256:Riboflavin; C014758:plumbagin