Diazepam binding inhibitor-like immunoreactivity(51-70): distribution in human brain, spinal cord and peripheral tissues.
We have used a specific radioimmunoassay to describe the distribution of diazepam binding inhibitor-like immunoreactivity (DBI-IR(51-70) in human post-mortem tissues. In brain, highest concentrations were found in the cerebellum, amygdala, hippocampus, hypothalamus and substantia nigra. In the spinal cord, DBI-IR(51-70) was evenly distributed. In peripheral tissues, highest concentrations were found in the liver and kidney. Chromatographic analysis revealed several molecular forms of DBI-IR(51-70) the major form being of greater molecular weight and hydrophobicity than the synthetic fragment peptide. In peripheral tissues, but not in the CNS, a small peak of immunoreactivity was indistinguishable from the synthetic peptide. DBI-IR(51-70) is therefore widespread, but tissue processing may be different.
Ball
J A
JA
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Ghatei
M A
MA
Sekiya
K
K
Krausz
T
T
Bloom
S R
SR
D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000378:metabolism*; D026261:Diazepam Binding Inhibitor; D005260:Female; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009479:Neuropeptides / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D013116:Spinal Cord / Q000378:metabolism*
D026261:Diazepam Binding Inhibitor; D009479:Neuropeptides; D010446:Peptide Fragments