pH-dependent uptake and macrofilaricidal effects of chloroquine on adult filarial parasites in vitro.
Uptake and macrofilaricidal effects of chloroquine (CQ) and other aminoquinolines were found to be highly pH dependent in Brugia pahangi, Acanthocheilonema viteae, Onchocerca volvulus, and Dirofilaria immitis. Using [3H]CQ, it was found that all of the parasites took up more drug under alkaline conditions (RPMI 1640 at pH 8.4) than in neutral (pH 7.4) or acidic (pH 6.8) media. Differences were seen in the amount of drug taken up among the filariae studied. B. pahangi and A. viteae took up 7 times more chloroquine per milligram of tissue than did O. volvulus, and 30 times more than D. immitis during a 60-min incubation period at pH 8.4. Sensitivity to the aminoquinolines also increased with increasing media pH, and was measured using parasite motility as an indicator of drug efficacy. Potency of chloroquine against B. pahangi increased 100-fold at pH 8.4 compared to pH 7.4. A. viteae and O. volvulus showed similar sensitivity to chloroquine compared to B. pahangi; D. immitis was less sensitive. While uptake of chloroquine was linear from pH 6.8 to 8.4, B. pahangi was unaffected by 32 microM of the drug below pH 7.6; at any pH above this, motility of this parasite was completely inhibited. Calculations of the internal pH of this parasite indicated that it shifted upwards significantly with changes in media pH. It was concluded that these shifts in internal pH may render parasites more sensitive to the effects of chloroquine.
VandeWaa
E A
EA
Department of Pharmacology and Toxicology, Michigan State University, East Lansing 48824.
Williams
J F
JF
Geary
T G
TG
D000818:Animals; D000871:Anthelmintics / Q000493:pharmacokinetics*; D002009:Brugia / Q000187:drug effects / Q000378:metabolism; D002738:Chloroquine / Q000493:pharmacokinetics / Q000494:pharmacology*; D004153:Dipetalonema / Q000187:drug effects / Q000378:metabolism; D004183:Dirofilaria immitis / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005369:Filaricides / Q000493:pharmacokinetics*; D005370:Filarioidea / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009854:Onchocerca / Q000187:drug effects / Q000378:metabolism
D000871:Anthelmintics; D005369:Filaricides; D002738:Chloroquine