title: Specific binding of nuclear proteins to the promoter region of a maize nuclear rRNA gene unit.
abstract: The external spacer region of a nuclear rRNA gene unit of maize was analyzed for binding of nuclear proteins and revealed binding to the promoter region and to the 200-base pair repeat elements upstream of the promoter. The promoter binding site was further characterized by DNase I and exonuclease III foot-printing as well as by gel shift and South-Western experiments, revealing the binding of several proteins. Competition experiments indicate that the protein which interacts with the repeat units also binds to the promoter region. A model for the combined action of both binding regions in the regulation of transcription is presented.
authors.affiliation: Institut für Biologie III, Albert-Ludwigs-Universität, Freiburg, Federal Republic of Germany.
authors.forename: M L
authors.initials: ML
authors.lastname: Schmitz
authors.forename: U G
authors.initials: UG
authors.lastname: Maier
authors.forename: J W
authors.initials: JW
authors.lastname: Brown
authors.forename: G
authors.initials: G
authors.lastname: Feix
mesh_terms: D001667:Binding, Competitive; D003850:Deoxyribonuclease I / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D011401:Promoter Regions, Genetic; D012335:RNA, Ribosomal / Q000032:analysis*; D012337:RNA, Ribosomal, 18S / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D009687:Nuclear Proteins; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D003850:Deoxyribonuclease I