Activation of protein kinase C induces a long-term depression of glutamate sensitivity of cerebellar Purkinje cells. An in vitro study.
In immature rat cerebellar slices in vitro, a long term depression (LTD) of the responses of Purkinje cells (PCs) to L-glutamate (Glu) was achieved in 30% of the recorded cells by simultaneous stimulation of the neurones by Glu and by climbing fibres (CFs). This effect was not observed for L-aspartate (Asp)-induced responses. Similarly, selective LTD of Glu-induced responses was obtained in 22% of the cells by pairing Glu applications with direct stimulation of the cells which elicited calcium spikes in these neurones. Finally, bath application of phorbol esters also induced a selective LTD of Glu-induced responses in all cells tested. These results suggest that protein kinase C is involved in cerebellar synaptic plasticity.
Crepel
F
F
Laboratoire de Neurobiologie et Neuropharmacologie du Développement, Université Paris-Sud, Orsay, France.
Krupa
M
M
D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D002531:Cerebellum / Q000502:physiology; D004553:Electric Conductivity; D004789:Enzyme Activation; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D009412:Nerve Fibers / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism*; D011689:Purkinje Cells / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D005971:Glutamates; D001224:Aspartic Acid; D018698:Glutamic Acid; D011493:Protein Kinase C