Characterization of one phenotype of human periodontal granulation-tissue fibroblasts.
Human granulation-tissue fibroblasts were cultured from oral chronic inflammatory lesions and compared with fibroblasts of healthy gingival connective tissue with respect to cell-surface sialoglycoproteins, and the synthesis of extracellular matrix components. Granulation-tissue fibroblasts exhibited a slower growth rate and larger size than their controls. Their cell-surface sialoglycoproteins resembled those of the control cells, except that the relative amount of glycoproteins in the 140-kd region was lower. The ratio of mRNAs for pro alpha l (I) and pro alpha l (III) collagen chains was decreased in granulation-tissue fibroblasts, although electrophoretic fractionation of the proteins did not reveal consistent differences in type I/type III collagen ratio. Granulation-tissue fibroblasts secreted into the culture medium a dermatan sulfate proteoglycan with a lower molecular weight. After digestion with chondroitinase ABC, the molecular weight of the core protein appeared to be identical with that of the control fibroblasts, suggesting a difference in the glycosylation of the core protein. These results support the theory that granulation-tissue fibroblasts represent a distinct phenotype of fibroblastic cells.
Larjava
H
H
Departments of Periodontology, University of Turku, Finland.
Heino
J
J
Kähäri
V M
VM
Krusius
T
T
Vuorio
E
E
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis; D003094:Collagen / Q000032:analysis / Q000235:genetics*; D003871:Dermatan Sulfate / Q000032:analysis; D005347:Fibroblasts / Q000378:metabolism; D006097:Granulation Tissue / Q000166:cytology*; D006801:Humans; D008875:Middle Aged; D010519:Periodontium / Q000166:cytology*; D010641:Phenotype; D012333:RNA, Messenger / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis
D011508:Chondroitin Sulfate Proteoglycans; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C032649:dermatan sulfate proteoglycan; D003871:Dermatan Sulfate; D003094:Collagen