title: cDNA cloning and nucleotide sequence of rat muscle-specific enolase (beta beta enolase).
abstract: The nucleotide sequence of rat muscle-specific enolase cDNA was determined by sequencing three cDNA clones encoding this enolase isozyme. The nearly full-length cDNA consists of 13-bp 5'- and 84-bp 3'-noncoding regions and a poly(A) tail in addition to a 1302-bp coding region encoding a polypeptide composed of 434 amino acid residues. The deduced primary structure of this enolase isozyme is about 80% similar to those determined previously for rat neuron-specific and non-neuronal enolase isozymes. Southern blot analysis suggested strongly the existence of a single copy of the muscle-specific enolase gene per haploid genome. The mRNA for this enolase isozyme was detected in rat skeletal muscle on day 1 after birth and its level increased rapidly during 10-30 days after birth without any change in its size (1500 bases).
authors.affiliation: Department of Biology, Faculty of Science, Niigata University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohshima
authors.forename: H
authors.initials: H
authors.lastname: Mitsui
authors.forename: Y
authors.initials: Y
authors.lastname: Takayama
authors.forename: E
authors.initials: E
authors.lastname: Kushiya
authors.forename: K
authors.initials: K
authors.lastname: Sakimura
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology*; D010751:Phosphopyruvate Hydratase / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping
chemical_list: D004247:DNA; D010751:Phosphopyruvate Hydratase