Cell-mediated lympholysis (CML) to allogeneic and trinitrophenyl (TNP)-modified cells: re-evaluation of the role of the thymus in CML.
The role of the thymus in cell-mediated lympholysis (CML) to allogeneic and trinitrophenyl (TNP)-modified cells was re-evaluated using adult thymectomized (ATx) and neonatal thymectomized (NTx) mice. CML to TNP-modified cells was used as the model of the major histocompatibility complex (MHC)-restricted CML. CML to TNP-modified cells, but not to allogeneic cells was reduced at late stages after ATx and remained low even in NTx-11 (thymectomy at 11 days of age) mice. Interleukin-2 (IL-2) producing activity in such mice was lower than that in the normal controls, while allogeneic CML was maintained at the same level as seen in the controls. The addition of exogenous IL-2 to in vitro culture led to a restoration of the generation of CML to TNP-modified cells, in neonatal thymectomized mice. These results suggested that thymus-dependency differs in the 2 forms of CML and that the CML to TNP-modified cells showed a higher thymus-dependency than did the allogeneic CML. Moreover, the activity of helper T cells may exert a direct influence on CML to TNP-modified cells, as compared to allogeneic cells.
Gondo
H
H
Tokuda
N
N
Taniguchi
K
K
Nomoto
K
K
D000818:Animals; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D007376:Interleukin-2 / Q000096:biosynthesis; D007519:Isoantigens / Q000276:immunology; D008211:Lymphocyte Cooperation; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D013934:Thymectomy; D013950:Thymus Gland / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D007376:Interleukin-2; D007519:Isoantigens; D014301:Trinitrobenzenes