Identification and characterization of a human cell line with dendritic cell features.
CORP-4 is a cell line obtained in our laboratory from an explanted human bladder carcinoma. This cell line shows certain dendritic cell features such as adherence to the culture plate surface, a doubling time of 24 h and an enzymatic profile typical of cells involved in antigen presentation (non-specific esterases, lysozyme and alpha-1-antitrypsin). Its phenotypic analysis revealed CD 15 and Fc receptor expression, S-100 surface protein and the presence of positive reactivity to different lectins such as Concanavalin A (Con A) and Peanut agglutinin (PNA). CORP-4 was found to be a non-phagocytic cell line after it was assayed with latex, and FcR- and C3bR-mediated phagocytosis. Furthermore, CORP-4 produced interleukin-1 (IL-1) as determined by thymocyte proliferation assays and also fixes immune complexes in a non-complement dependent fashion. HLA class I and class II antigens were inducible by both 5 azacytidine and gamma interferon.
Santamaria
M
M
Department of Biochemistry, School of Medicine, Reina Sofia Hospital, University of Cordoba Menendez Pidal, Spain.
Molina
I
I
Muñoz
E
E
Lopez
A
A
Toro
M
M
Peña
J
J
D000372:Agglutination Tests; D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000032:analysis / Q000166:cytology* / Q000276:immunology; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D051379:Mice; D008809:Mice, Inbred C3H; D010587:Phagocytosis; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology
D006649:Histocompatibility Antigens; D007375:Interleukin-1