Studies of a glomerular permeability factor in patients with minimal-change nephrotic syndrome.
Peripheral blood mononuclear cells (PBMC) from patients with minimal-change nephrotic syndrome (MCNS) were tested for their ability to produce a factor which increases the urinary protein excretion levels of rats. It was shown that enhanced proteinuria can be produced in 8-hour urine specimens from rats by the injection of concentrated supernatants of cultured concanavalin-A-stimulated PBMC of patients with MCNS, but not from other nephrotics or normal subjects. The increase in urinary protein excretion was associated with a significant alteration of glomerular epithelial cells similar to that seen in MCNS. These results suggest that in MCNS, PBMC release a factor, which we termed a glomerular permeability factor (GPF), causing changes in glomerular permeability with resulting proteinuria.
Yoshizawa
N
N
Second Department of Internal Medicine, National Defense Medical College, Tokorozawa, Japan.
Kusumi
Y
Y
Matsumoto
K
K
Oshima
S
S
Takeuchi
A
A
Kawamura
O
O
Kubota
T
T
Kondo
S
S
Niwa
H
H
D000293:Adolescent; D000328:Adult; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006801:Humans; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000648:ultrastructure; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009402:Nephrosis, Lipoid / Q000097:blood*; D010539:Permeability; D011507:Proteinuria / Q000209:etiology / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D014556:Urine
D003208:Concanavalin A