title: Influence of thyroid status on the membranes of rat liver mitochondria. Unique localization of L-glycerol-3-phosphate dehydrogenase.
abstract: The effect of thyroid status on the physical properties of rat liver mitochondrial membranes and on the lipid microenvironment of proteins was investigated. The steady-state fluorescence anisotropy of diphenyl-1,3,5-triene and 1-[4-(trimethylaminophenyl)phenyl]-6-phenylhexa-1,3,5-triene revealed an increase of the order of the membranes with the increase of hormone level. Protein arrangement in the inner mitochondrial membrane altered with the thyroid status, which was reflected by digitonin subfractionation of mitochondria. The microenvironment of FAD-linked L-glycerol-3-phosphate dehydrogenase was dramatically influenced by thyroxine.
authors.affiliation: Institute of Enzymology, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Beleznai
authors.forename: E
authors.initials: E
authors.lastname: Amler
authors.forename: H
authors.initials: H
authors.lastname: Rauchová
authors.forename: Z
authors.initials: Z
authors.lastname: Drahota
authors.forename: V
authors.initials: V
authors.lastname: Jancsik
mesh_terms: D000818:Animals; D004072:Digitonin; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats; D012995:Solubility; D013959:Thyroid Diseases / Q000378:metabolism*; D013963:Thyroid Hormones / Q000502:physiology*
chemical_list: D005456:Fluorescent Dyes; D013963:Thyroid Hormones; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; D005993:Glycerolphosphate Dehydrogenase; D004072:Digitonin