Comparison of modified Bordet-Gengou and modified Regan-Lowe media for the isolation of Bordetella pertussis and Bordetella parapertussis.
Culture and fluorescent-antibody methods for detection of Bordetella species were evaluated by two state public health laboratories. Field-inoculated plates of Regan-Lowe agar medium were most useful if incubation was initiated on the day of collection. Regan-Lowe and Bordet-Gengou media were comparable for subculturing nasopharyngeal specimens that were transported and enriched in half-strength Regan-Lowe agar. Maximum sensitivity was achieved when the media were used in parallel. Fluorescent-antibody-stained smears of nasopharyngeal specimens were more sensitive for detection of Bordetella pertussis than for detection of Bordetella parapertussis. The fluorescent-antibody method, however, was too insensitive for use without culture.
Kurzynski
T A
TA
Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison 53706.
Boehm
D M
DM
Rott-Petri
J A
JA
Schell
R F
RF
Allison
P E
PE
D001884:Bordetella / Q000254:growth & development / Q000302:isolation & purification*; D001886:Bordetella pertussis / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D003581:Cytodiagnosis / Q000379:methods; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology; D014917:Whooping Cough / Q000175:diagnosis*
D003470:Culture Media