title: Sustained nonoxidative glucose utilization and depletion of glycogen in reperfused canine myocardium.
abstract: Ischemically injured reperfused myocardium is characterized by increased 18F-fluorodeoxyglucose uptake as demonstrated by positron emission tomography. To elucidate the metabolic fate of exogenous glucose entering reperfused myocardium, D-[6-14C] glucose and L-[U-13C] lactate were used to determine glucose uptake, glucose oxidation and the contribution of exogenous glucose to lactate production. The pathologic model under investigation consisted of a 3 h balloon occlusion of the left anterior descending coronary artery followed by 24 h of reperfusion in canine myocardium. The extent and severity of myocardial injury after the ischemia and reperfusion were assessed by histochemical evaluation (triphenyltetrazolium chloride and periodic acid-Schiff stains). Thirteen intervention and four control dogs were studied. The glucose uptake in the occluded/reperfused area was significantly enhanced compared with that in control dogs (0.40 +/- 0.14 versus 0.15 +/- 0.10 mumol/ml, respectively). In addition, a significantly greater portion of the glucose extracted immediately entered glycolysis in the intervention group (75%) than in the control dogs (33%). The activity of the nonoxidative glycolytic pathway was markedly increased in the ischemically injured reperfused area, as evidenced by the four times greater lactate release in this area compared with the control value. The dual carbon-labeled isotopes showed that 57% of the exogenous glucose entering glycolysis was being converted to lactate. Exogenous glucose contributed to greater than 90% of the observed lactate production. This finding was confirmed by the histochemical finding of sustained glycogen depletion in the occlusion/reperfusion area. The average area of glycogen depletion (37%) significantly exceeded the average area of necrosis (17%). These data demonstrate enhanced and sustained activity of the nonoxidative glycolytic pathway after a prolonged occlusion with reperfusion in canine myocardium. Because glycogen stores remain depleted, exogenous glucose becomes an important myocardial substrate under these pathologic conditions.
authors.affiliation: Division of Cardiology, University of California, San Francisco.
authors.forename: M
authors.initials: M
authors.lastname: Schwaiger
authors.forename: R A
authors.initials: RA
authors.lastname: Neese
authors.forename: L
authors.initials: L
authors.lastname: Araujo
authors.forename: W
authors.initials: W
authors.lastname: Wyns
authors.forename: J A
authors.initials: JA
authors.lastname: Wisneski
authors.forename: H
authors.initials: H
authors.lastname: Sochor
authors.forename: S
authors.initials: S
authors.lastname: Swank
authors.forename: D
authors.initials: D
authors.lastname: Kulber
authors.forename: C
authors.initials: C
authors.lastname: Selin
authors.forename: M
authors.initials: M
authors.lastname: Phelps
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002250:Carbon Radioisotopes; D004285:Dogs; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D006439:Hemodynamics; D007773:Lactates / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D010084:Oxidation-Reduction
chemical_list: D002247:Carbon Isotopes; D002250:Carbon Radioisotopes; D007773:Lactates; D006003:Glycogen; D005947:Glucose