A simplified technique for the short-term tissue culture of rabbit corneal cells.
A technique for the short-term culture of pure populations of rabbit corneal endothelial and epithelial cells has been developed. Rabbit corneas were placed on concave agarose surfaces, treated briefly with a solution of trypsin and ethylenediamine tetracetic acid, and transferred, either epithelial cell surface or endothelial cell surface down, to microscope slide culture chambers. Within 6 to 12 h the epithelial cells or endothelial cells attached to the slide chamber surface and the cornea was removed, leaving behind a pure population of cells which spread out and grew to fill the surface of the slide chamber. This technique provides a simple and economic means for the reproducible initiation of primary cultures of rabbit corneal epithelial and endothelial cells for us in a variety of experiments.
Xie
L
L
LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112.
Gebhardt
B M
BM
D000818:Animals; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology*; D046508:Culture Techniques / Q000379:methods; D004727:Endothelium / Q000166:cytology; D004847:Epithelial Cells; D011817:Rabbits; D012685:Sepharose; D014357:Trypsin / Q000008:administration & dosage
D012685:Sepharose; D014357:Trypsin