Analytical problems encountered during high-performance liquid chromatographic separation and coulometric detection of bopindolol metabolites in human plasma.
Good clinical control of hypertension is achieved with low doses of the potent beta-blocker bopindolol. Pharmacokinetic evaluations therefore require an analytical technique of high sensitivity. Analysis of plasma by high-performance liquid chromatography (HPLC) using electrochemical detection provides this sensitivity. This article describes the development of an analytical procedure and presents a reversed-phase HPLC method with coulometric detection suitable for plasma analyses during pharmacokinetic investigations of bopindolol therapy.
Perkins
S L
SL
Division of Biochemistry, Ottawa Civic Hospital, Ontario, Canada.
Tattrie
B
B
Johnson
P M
PM
Rabin
E Z
EZ
D000319:Adrenergic beta-Antagonists / Q000097:blood* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D010869:Pindolol / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D012015:Reference Standards; D012997:Solvents
D000319:Adrenergic beta-Antagonists; D012997:Solvents; D010869:Pindolol; C036007:bopindolol