title: DNA cell cycle studies in uveal melanoma.
abstract: We histologically studied uveal melanomas treated with surgery only (enucleation or ciliochoroidectomy), low-dose (20 Gy) preenucleation radiation followed by enucleation, or enucleated melanomas after high-dose (50 to 80 Gy) charged-particle beam therapy. There was significantly less bromodeoxyuridine uptake in irradiated vs nonradiated melanomas (P less than .0001). Similarly, tissue culture growth of irradiated tumors was significantly less (P less than .007). These data demonstrate destruction of reproductive integrity of helium ion-irradiated melanomas. The incorporation of bromodeoxyuridine and fine needle aspiration biopsy techniques may be useful in the delineation of successfully irradiated tumors from tumors with apparent growth secondary to radiation vasculopathy.
authors.affiliation: Department of Ophthalmology, Francis I. Proctor Foundation, University of California, San Francisco 94143.
authors.forename: D H
authors.initials: DH
authors.lastname: Char
authors.forename: K
authors.initials: K
authors.lastname: Huhta
authors.forename: F
authors.initials: F
authors.lastname: Waldman
mesh_terms: D000328:Adult; D000368:Aged; D002453:Cell Cycle; D004247:DNA / Q000502:physiology*; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000532:radiotherapy; D008875:Middle Aged; D014604:Uveal Neoplasms / Q000235:genetics* / Q000532:radiotherapy
chemical_list: D004247:DNA