An immunoassay of human band 5 ("tartrate-resistant") acid phosphatase that involves the use of anti-porcine uteroferrin antibodies.
We describe an immunoassay for human band-5 acid phosphatase in which antibodies to porcine uteroferrin, immobilized on Sepharose particles, are used. Band-5 acid phosphatase is the tartrate-resistant isoenzyme normally expressed in tissue macrophages such as osteoclasts and alveolar macrophages. The immunoassay is similar in reproducibility and sensitivity to assays based on inhibition by d-tartrate. However, compared with the latter, the greater specificity of the immunoassay makes it markedly less susceptible to errors arising from the presence of non-band-5 acid phosphatases, e.g., from prostate.
Whitaker
K B
KB
Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Cox
T M
TM
Moss
D W
DW
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood*; D000818:Animals; D000906:Antibodies; D006801:Humans; D007118:Immunoassay; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000097:blood*; D008264:Macrophages / Q000201:enzymology; D008297:Male; D008667:Metalloproteins / Q000276:immunology*; D011467:Prostate / Q000201:enzymology; D011650:Pulmonary Alveoli / Q000166:cytology; D011786:Quality Control; D012016:Reference Values; D013154:Spleen / Q000201:enzymology; D013552:Swine; D000071681:Tartrate-Resistant Acid Phosphatase; D013644:Tartrates / Q000494:pharmacology
D000906:Antibodies; D007527:Isoenzymes; D008667:Metalloproteins; D013644:Tartrates; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; C029768:tartaric acid