Identity of the E-series prostaglandin produced by equine chondrocytes and synovial cells in response to a variety of stimuli.
Although an E-series prostaglandin has previously been identified in equine inflammatory exudate, the identity of this eicosanoid as PGE2 has not been confirmed. The objective of this study was the specific characterisation of the prostaglandin produced by equine cells in the presence of an inflammatory stimulus. By using two radioimmunoassays, a relatively non-specific PGE2 assay and a more specific PGE1 assay, it has been possible to identify the E-series prostaglandin produced by equine chondrocytes and synovial cells, in response to a variety of stimuli, as PGE2.
May
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SA
Department of Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Hatfield, Hertfordshire.
Hooke
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Lees
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P
D000527:Alprostadil / Q000032:analysis / Q000096:biosynthesis; D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000032:analysis* / Q000096:biosynthesis; D006736:Horses; D007249:Inflammation / Q000378:metabolism*; D011863:Radioimmunoassay; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*
D000527:Alprostadil; D015232:Dinoprostone