Characterization of very low density lipoprotein from Watanabe heritable hyperlipidemic rabbits.
We investigated the properties of very low density lipoprotein (VLDL) from two types of Watanabe heritable hyperlipidemic (WHHL) rabbits: one with a high incidence of coronary atherosclerosis (type 1), and the other with a low incidence (type 2). When incubated with mouse peritoneal macrophages, VLDL from type 1 WHHL rabbit (type 1-VLDL) stimulated cholesteryl ester synthesis 10.5-fold more than VLDL from the type 2 WHHL rabbit (type 2-VLDL) did. Moreover, a similar difference was seen in the stimulation of cholesteryl ester synthesis in peritoneal macrophages isolated from the WHHL rabbits. The mass ratios of cholesterol to protein in type 1- and type 2-VLDL were 5.69 and 2.05, respectively. Agarose gel electrophoresis of type 1-VLDL showed beta mobility, and that of type 2-VLDL showed pre-beta mobility. No difference was seen between the sizes of VLDL particles of the two types. The amount of apolipoprotein E in type 1-VLDL was greater than that in type 2-VLDL. In conclusion, the difference between type 1 and type 2 WHHL rabbits is at least partly due to the presence in type 1 animals of VLDL particles rich in cholesteryl esters and apolipoprotein E, particles which are very similar to beta-VLDL in conformation.
Ishii
K
K
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Kita
T
T
Yokode
M
M
Kume
N
N
Nagano
Y
Y
Otani
H
H
Yamamura
T
T
Murayama
S
S
Morimoto
Y
Y
Teranishi
Y
Y
D000818:Animals; D001057:Apolipoproteins E / Q000032:analysis; D002788:Cholesterol Esters / Q000096:biosynthesis*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004587:Electrophoresis, Agar Gel; D005260:Female; D006949:Hyperlipidemias / Q000097:blood*; D007525:Isoelectric Focusing; D008079:Lipoproteins, VLDL / Q000097:blood*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D011817:Rabbits
D001057:Apolipoproteins E; D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; D008079:Lipoproteins, VLDL