Semicarbazide-sensitive amine oxidase activity in catfish tissues.
1. Semicarbazide-sensitive amine oxidase activity was found in five tissues of the siluroid catfish, Parasilurus asotus, using benzylamine as substrate. It was highest in the intestine, followed by the liver and skin. 2. The apparent Km value and optimal pH for benzylamine in the intestine were 49.8 microns and 8.8, respectively. 3. Substrate specificity of the enzyme was tested in the intestine and the highest activity was found with beta-phenylethylamine, followed by tryptamine and benzylamine.
Kumazawa
T
T
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
Seno
H
H
Suzuki
O
O
D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D001596:Benzylamines; D002397:Catfishes / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010627:Phenethylamines; D012663:Semicarbazides / Q000494:pharmacology*; D013379:Substrate Specificity; D014018:Tissue Distribution
D001596:Benzylamines; D010627:Phenethylamines; D012663:Semicarbazides; C029261:phenethylamine; C010059:carbamylhydrazine; C030796:benzylamine; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors