title: Purification and characterization of two classes of neurotoxins from the funnel web spider, Agelenopsis aperta.
abstract: Two classes of paralytic toxins were isolated from the venom of Agelenopsis aperta and their chemical and larvicidal properties characterized. Five acylpolyamine toxins (alpha-agatoxins) of molecular masses 452, 488, 489, 504, and 505 Da produce immediate but reversible paralysis in Manduca sexta following injection. Six insecticidal peptides (mu-agatoxins) produce a gradual but irreversible paralysis. The complete amino acid sequences (36-38 residues) of the mu-agatoxins are presented. These peptides contain eight half-cystines and are quite similar in sequence. At least four of these toxins are amidated at the carboxyl terminus. The secondary structure of one of these toxins (mu-Aga V) was investigated.
authors.affiliation: Zoecon Research Institute, Sandoz Crop Protection, Palo Alto, California 94304.
authors.forename: W S
authors.initials: WS
authors.lastname: Skinner
authors.forename: M E
authors.initials: ME
authors.lastname: Adams
authors.forename: G B
authors.initials: GB
authors.lastname: Quistad
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: B J
authors.initials: BJ
authors.lastname: Cesarin
authors.forename: F E
authors.initials: FE
authors.lastname: Enderlin
authors.forename: D A
authors.initials: DA
authors.lastname: Schooley
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001180:Arthropod Venoms / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009036:Moths; D009498:Neurotoxins; D011487:Protein Conformation; D013111:Spider Venoms / Q000145:classification / Q000302:isolation & purification* / Q000633:toxicity
chemical_list: D000596:Amino Acids; D001180:Arthropod Venoms; D009498:Neurotoxins; D013111:Spider Venoms