Effects of alloxan-induced diabetes on somatostatin binding to cytosolic components of rabbit gastric fundic mucosa.
Diabetes was induced by administration of alloxan (150 mg/Kg) to 24 h-fasted rabbits. Somatostatin-like immunoreactivity (SLI) and cytosolic binding sites for somatostatin in gastric fundic mucosa were studied using radiolabelled Tyr11-somatostatin. Three months after the onset of the disease, the specific binding of somatostatin to these sites was seen to be significantly lower, due to a reduction in the number (but not the affinity) of specific somatostatin binding sites of high-affinity and a disappearance of the specific somatostatin binding sites of low-affinity. These changes were associated with an increase in the SLI concentration in both gastric fundic mucosa and plasma.
Guijarro
L G
LG
Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Alcala de Henares, Madrid, Spain.
Lopez-Ruiz
M P
MP
Prieto
J C
JC
Arilla
E
E
D000818:Animals; D003600:Cytosol / Q000378:metabolism / Q000648:ultrastructure*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005748:Gastric Fundus / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D011817:Rabbits; D011863:Radioimmunoassay; D013004:Somatostatin / Q000097:blood / Q000276:immunology / Q000378:metabolism*
D013004:Somatostatin