title: Combined thin layer chromatography/mass spectrometry: an application of 252Cf plasma desorption mass spectrometry for drug monitoring.
abstract: The pharmacokinetic analysis is performed in a three-step procedure: sample extraction, sample purification by thin layer chromatography (TLC) and quantitative sample detection by time-of-flight (TOF) mass spectrometry. 252Cf plasma desorption (PD) mass spectrometry utilizing the fission fragment-induced ionization and desorption of non-volatile compounds is suitable as a universal, non-destructive detector in TLC. Here TLC and mass spectrometry are operated in an off-line combination. As an example some pharmacokinetic data for etoposide (VP 16-213) together with calibration data are presented. The new experimental method is discussed in terms of sensitivity and detection limit.
authors.forename: H
authors.initials: H
authors.lastname: Danigel
authors.forename: L
authors.initials: L
authors.lastname: Schmidt
authors.forename: H
authors.initials: H
authors.lastname: Jungclas
authors.forename: K H
authors.initials: KH
authors.lastname: Pflüger
mesh_terms: D002142:Californium; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005047:Etoposide / Q000097:blood*; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry; D011034:Podophyllotoxin / Q000031:analogs & derivatives*
chemical_list: D005047:Etoposide; D002142:Californium; D011034:Podophyllotoxin