title: Hormone synthesis in human thyroglobulin: possible cleavage of the polypeptide chain at the tyrosine donor site.
abstract: At moderate iodination levels (20 iodine atoms/mol) human thyroglobulin (hTg) produces after reduction a hormone-rich peptide of 26 kDa which contains the preferential hormonogenic 'acceptor' tyrosine (Tyr 5) of the protein. The site of cleavage of the hTg chain was demonstrated by analysis of the 26 kDa tryptic hydrolysis products. It consistently yielded the peptide Gln-82-Val-129 which consequently made it possible to localize the hTg chain cleavage at tyrosine residue 130. Evidence for tyrosine involvement in hTg cleavage during thyroid hormone formation supports the hypothesis that peptide bond cleavage would occur at the 'donor' tyrosine residue and suggests that tyrosine 130 would be the donor site reacting with the major hormone-forming acceptor site (Tyr 5) of hTg.
authors.affiliation: UA 178 CNRS et U 38 INSERM, Biochimie Médicale, Faculté de Médecine, Marseille, France.
authors.forename: C
authors.initials: C
authors.lastname: Marriq
authors.forename: P J
authors.initials: PJ
authors.lastname: Lejeune
authors.forename: N
authors.initials: N
authors.lastname: Venot
authors.forename: L
authors.initials: L
authors.lastname: Vinet
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D007467:Iodoproteins / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D013954:Thyroglobulin / Q000378:metabolism*; D013963:Thyroid Hormones / Q000096:biosynthesis*; D014443:Tyrosine
chemical_list: D007467:Iodoproteins; D010446:Peptide Fragments; D013963:Thyroid Hormones; D014443:Tyrosine; D013954:Thyroglobulin