Duck hepatitis B virus: cloning and subcloning of the viral genome.
In the course of studies on the biology of hepadnavirus infections, duck hepatitis B virus (DHBV) DNA was isolated from the serum of a German Pekin duck. Viral DNA was cloned in E. coli using pBR 322 DNA as a vector. The cloned DHBV DNA F 1-6 was characterised by restriction enzyme analyses. DHBV DNA F 1-6 was subcloned in both orientations in plasmid pSP 65 to produce strand-specific RNA probes. These probes specifically identified asymmetrically replicating nascent minus-strand DHBV DNA species or plus-strand viral RNA transcripts.
Oexle
K
K
Department of Medicine, University of Freiburg.
Blum
H E
HE
Walter
E
E
Offensperger
W B
WB
Offensperger
S
S
Grundmann
H
H
Teubner
K
K
Gerok
W
W
D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004372:Ducks; D005814:Genes, Viral; D005822:Genetic Vectors; D006509:Hepatitis B / Q000382:microbiology / Q000662:veterinary*; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011201:Poultry Diseases / Q000382:microbiology*; D015347:RNA Probes; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014779:Virus Replication
D004279:DNA, Viral; D015347:RNA Probes; D012367:RNA, Viral