Biotin deficiency and susceptibility to fatty liver and kidney syndrome in broiler chicks: reduced 6-phosphofructokinase (EC 2.7.1.11) activity but normal fructose 2,6-bisphosphate content in birds with hepatomegaly.
In two separate experiments, using different strains, broiler chicks were reared on either a commercial-type chick mash (control) or a fatty liver and kidney syndrome (FLKS)-inducing diet. In Expt a, chicks were killed on day 29 and in Expt b, on day 32. Body-weights and liver weights were measured, and values from those given the control ration used to construct a hepatomegaly index by employing a variant of linear discriminant analysis. Application of the index to FLKS birds revealed a statistically significant bimodal distribution of liver size. The birds with enlarged livers (high index) also possessed metabolic abnormalities in that 6-phosphofructokinase (EC 2.7.1.11; PFK-1) activity (measured at low substrate concentration) was depressed despite the presence of normal, or even slightly elevated fructose 2,6-bisphosphate concentration. This indicates the presence of an uncharacterized regulatory mechanism for PFK-1 in FLKS-susceptible birds.
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D000818:Animals; D001710:Biotin / Q000172:deficiency*; D002645:Chickens; D005234:Fatty Liver / Q000209:etiology / Q000662:veterinary*; D005260:Female; D005635:Fructosediphosphates / Q000032:analysis*; D006529:Hepatomegaly / Q000378:metabolism / Q000662:veterinary*; D006598:Hexosediphosphates / Q000032:analysis*; D007674:Kidney Diseases / Q000209:etiology / Q000662:veterinary*; D010732:Phosphofructokinase-1 / Q000032:analysis*; D011201:Poultry Diseases / Q000209:etiology / Q000378:metabolism*
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D001710:Biotin; C027652:fructose 2,6-diphosphate; D010732:Phosphofructokinase-1