Tumor cell recruitment in the mouse adenocarcinoma EO 771 directly demonstrated by double labeling with [3H]- and [14C] thymidine and flow cytometry.
Tumor cell recruitment in the mouse adenocarcinoma EO 771 after application of 1-beta-D-arabinofuranosylcytosine (AraCyt) has been directly demonstrated by double labeling with [3H]- and [14C]-thymidine. This method enables a quantitative study of the extent and time course of tumor cell recruitment in this solid mouse tumor. The results show that tumor cell recruitment is a continuous process that starts early after AraCyt application (about 4-8 h) and lasts about 1 day with a maximum at about 12 h after Ara-Cyt application. Up to 40% of all cells are recruited at that time. The recruited tumor cells re-enter the resting state after having passed through one cycle.
Walter
J
J
Institut für Medizinische Strahlenkunde, Universität Würzburg, Federal Republic of Germany.
Maurer-Schultze
B
B
D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology*; D000818:Animals; D002250:Carbon Radioisotopes; D002455:Cell Division / Q000187:drug effects*; D003561:Cytarabine / Q000627:therapeutic use*; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014316:Tritium
D002250:Carbon Radioisotopes; D003561:Cytarabine; D014316:Tritium