Taq I restriction polymorphism of HLA class-I genes, and hybridization with HLA-A and HLA-B specific probes.
Restriction enzyme site polymorphisms for Taq I endonuclease are examined in a panel of DNAs of HLA-typed individuals and families. Using a full-length DNA probe, 13 polymorphic bands were detected in a total of 31 restriction fragments analyzed. Only one of them (the 3.5-kb polymorphic band) correlated absolutely with serological allele B27, as already established. The A- and B-specific probes recognized only a very small subset (4 for the A probe, and 2 for the B probe) of the DNA fragments which were hybridized with the full-length DNA probe. For each of these two loci, a frequent polymorphism was described, which correlated with serologic haplotypes in several families studied.
Huetz
F
F
Laboratoire de Génétique Moléculaire, CNTS-Institut, Paris, France.
Mariotti
M
M
Lucotte
G
G
D000483:Alleles; D015343:DNA Probes, HLA; D015252:Deoxyribonucleases, Type II Site-Specific; D015698:Genomic Library; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D015793:HLA-B7 Antigen / Q000235:genetics; D006801:Humans; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length
D015343:DNA Probes, HLA; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015793:HLA-B7 Antigen; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases