Repair capacity of fertilized mouse eggs for X-ray damage induced in sperm and mature oocytes.
To study the repair capacity of fertilized mouse eggs for X-ray damage induced in sperm and mature oocytes, the potentiating effects of 3 well-known repair inhibitors, arabinofuranosyl cytosine (ara-C), 3-aminobenzamide (3AB) and caffeine, on the frequency of induced chromosome aberrations were examined in eggs fertilized with X-irradiated sperm or in eggs irradiated with X-rays at the mature oocyte stage immediately before fertilization. Gametic treatment, fertilization and embryo culture were carried out in vitro. Ara-C treatment was done only in the pre-DNA replication period, while treatment with 3AB and caffeine was continuous from fertilization to the first-cleavage metaphase. The induction of chromosome aberrations by exposing sperm or oocytes to X-rays was remarkably potentiated by post-treatment incubation in the presence of each of the 3 inhibitors. This result indicates the possibility that X-ray damage induced in sperm or oocytes is reparable in the fertilized eggs and that various types of repair processes are involved.
Matsuda
Y
Y
Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
Tobari
I
I
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002970:Cleavage Stage, Ovum / Q000502:physiology*; D003561:Cytarabine / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects; D008297:Male; D051379:Mice; D008940:Mitotic Index; D009865:Oocytes / Q000528:radiation effects*; D013094:Spermatozoa / Q000528:radiation effects*; D014965:X-Rays
D001549:Benzamides; D003561:Cytarabine; D002110:Caffeine; C025160:3-aminobenzamide