title: Improved determination of vanadium in biological fluids by electrothermal atomic absorption spectrometry.
abstract: We made sensitive and accurate electrothermal atomic absorption spectrometric measurements of vanadium in small volumes of serum. The wet-digested sample was extracted into an organic solvent, with N-benzoyl-N-(o-tolyl)hydroxylamine (BTA) as the chelating reagent. After evaporating the solvent, we dissolved the residue in acetic acid, and injected a 60-microL aliquot into a graphite furnace. In this way we could measure vanadium concentrations as low as 80 ng/L in 4 mL of serum. The within-run CV was 3.3% for serum, 7.7% for urine. Analytical recoveries of vanadium added to serum and urine were 90.3% and 90.8%, respectively. We measured vanadium concentrations in sera from 64 healthy persons (group 1), 15 nondialyzed uremic patients (group 2), and 11 hemodialyzed patients (group 3). The highest concentration of vanadium in group 1 was 240 ng/L; about 60% of the values for this group were less than 80 ng/L. In group 3, the vanadium concentrations were extremely high (15 +/- 14.2 micrograms/L, mean +/- SD), less so (but still above normal) in group 2 (1.58 +/- 3.16 micrograms/L).
authors.affiliation: Kitasato Biochemical Laboratories, Bristol-Myers, Kanagawa, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Ishida
authors.forename: K
authors.initials: K
authors.lastname: Kihira
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukamoto
authors.forename: F
authors.initials: F
authors.lastname: Marumo
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D011786:Quality Control; D012016:Reference Values; D006435:Renal Dialysis; D013054:Spectrophotometry, Atomic; D014462:Ultrafiltration; D014639:Vanadium / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D014639:Vanadium