title: Dimorphism-associated changes in intracellular pH of Candida albicans.
abstract: Intracellular pH (pHi) was monitored during pH-regulated dimorphism of Candida albicans using two different methods: (1) by steady-state distribution of propionic acid and (2) by use of polyene antibiotic, nystatin. There was no significant change in pHi during the first 120 min in either bud- or germ tube-forming populations. However, there was a rapid increase around 135 min which also coincided with the time of evagination. The magnitude of increase in pHi was different in the two populations; being 0.44 and 0.14 pH units in bud- and germ tube-forming populations, respectively. In the two diverging populations, the transient increase in pHi was followed by a rapid drop. The sharp rise in pHi of the population destined to form buds was sensitive to orthovanadate and to the depletion of K+ from the medium while this was not the case with germ tube-forming cells. The results suggest that pHi may play an important role in the phenotypic divergence of C. albicans.
authors.affiliation: Membrane Biology Unit, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: S
authors.initials: S
authors.lastname: Kaur
authors.forename: P
authors.initials: P
authors.lastname: Mishra
authors.forename: R
authors.initials: R
authors.lastname: Prasad
mesh_terms: D002176:Candida albicans / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D002462:Cell Membrane / Q000502:physiology; D003593:Cytoplasm / Q000502:physiology; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009761:Nystatin / Q000494:pharmacology; D010641:Phenotype; D011188:Potassium / Q000502:physiology; D011422:Propionates / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology
chemical_list: D011422:Propionates; D009761:Nystatin; D014638:Vanadates; D006180:Proton-Translocating ATPases; C029658:propionic acid; D011188:Potassium