title: Trypanosoma brucei contains two RNA polymerase II largest subunit genes with an altered C-terminal domain.
abstract: We have identified and cloned four trypanosomal RNA polymerase largest subunit genes. Here, we present the molecular analysis of two genes, Trp4.8 and Trp5.9. The sequence of these genes shows that they are almost identical to each other and indicates that they encode the largest subunit of RNA polymerase II. Both genes contain a C-terminal extension that is clearly distinct from that of other eukaryotic RNA polymerase II genes, because it lacks the common tandemly repeated heptapeptide sequence and is rich in acidic amino acids. It shares many potential phosphorylation sites, however, with the C-terminal extension of other eukaryotic RNA polymerase II large subunits. The presence of two RNA polymerase II loci suggests that a fourth RNA polymerase could be formed. Interestingly, the fourth gene is only found in species exhibiting antigenic variation.
authors.affiliation: Max-Planck-Institut für Biologie, Molecular Parasitology Unit, Tübingen, Federal Republic of Germany.
authors.forename: R
authors.initials: R
authors.lastname: Evers
authors.forename: A
authors.initials: A
authors.lastname: Hammer
authors.forename: J
authors.initials: J
authors.lastname: Köck
authors.forename: W
authors.initials: W
authors.lastname: Jess
authors.forename: P
authors.initials: P
authors.lastname: Borst
authors.forename: S
authors.initials: S
authors.lastname: Mémet
authors.forename: A W
authors.initials: AW
authors.lastname: Cornelissen
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D005796:Genes; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000235:genetics* / Q000494:pharmacology; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics*
chemical_list: D000546:Amanitins; D012319:RNA Polymerase II