title: Disassembly of the nuclear envelope of spisula oocytes in a cell-free system.
abstract: Nuclei isolated from oocytes of the surf clam Spisula solidissima are disassembled when exposed to extracts from maturing oocytes. In the course of this process the nuclear lamina undergoes a marked reduction in size and the nuclear membrane appears to be fragmented into vesicles. These events are accompanied by extensive phosphorylation of the oocyte 67-kDa lamin and its solubilization. The changes observed are similar to those which occur in vivo in activated Spisula oocytes. Nuclear envelope breakdown in vitro requires ATP and Mg2+, but not Ca2+. It is not affected by protease inhibitors and is inhibited by alkaline phosphatase.
authors.affiliation: Department of Cell Biology and Anatomy, Northwestern University Medical School, Chicago, Illinois 60611.
authors.forename: G
authors.initials: G
authors.lastname: Dessev
authors.forename: R
authors.initials: R
authors.lastname: Palazzo
authors.forename: L
authors.initials: L
authors.lastname: Rebhun
authors.forename: R
authors.initials: R
authors.lastname: Goldman
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000494:pharmacology; D000818:Animals; D049872:Bivalvia / Q000648:ultrastructure*; D002474:Cell-Free System; D002850:Chromatography, Gel; D034882:Lamins; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism; D017830:Octoxynol; D009865:Oocytes / Q000648:ultrastructure*; D010766:Phosphorylation; D011092:Polyethylene Glycols / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology
chemical_list: D034882:Lamins; D009687:Nuclear Proteins; D011480:Protease Inhibitors; D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D017830:Octoxynol; C010615:Nonidet P-40; D000469:Alkaline Phosphatase; D008274:Magnesium