An assessment of framelapse videography of the effect of cryopreservation on human sperm motility.
Sixteen sperm specimens collected from regular healthy donors at Groote Schuur Hospital Sperm Bank were assessed subjectively by phase contrast light microscopy and the results were compared with assessments made by framelapse videography. The same specimens were frozen and thawed and re-assessed by framelapse videography and the results compared with the pre-freeze assessments. The objective cryosurvival was found to be 53%. The subjective assessment varied by an average of 20% from the videographic method. Framelapse videography was found to be a practical reproducible method for assessment of sperm motility where accuracy is essential.
Wiswedel
K
K
Department of Obstetrics and Gynaecology, University of Cape Town.
Samuels
K S
KS
Bosschieter
J R
JR
Levin
M R
MR
D005615:Freezing; D006801:Humans; D008297:Male; D008853:Microscopy; D011309:Preservation, Biological; D013081:Sperm Motility; D014743:Videotape Recording