title: All species of the genus Bordetella contain genes for pertussis toxin of Bordetella pertussis.
abstract: A molecular probe for the PT-operon of B. pertussis hybridized with 4.7 Kb EcoRI-fragments of chromosomal DNAs of B. pertussis strain 475 phase I, phase IV, B. parapertussis strains 504 and 17903, B. bronchiseptica strain 214, B. parapertussis strain 17903-convertant of B. pertussis phage 134 but not with phage 134 DNA under stringent conditions of DNA-DNA hybridization. This fact indicates the presence of PT-genes in all Bordetella species. Since there is no production of PT in B. parapertussis and B. bronchiseptica, a presence of regulatory mutations in the PT-operon or absence of the functionally active vir-gene product in these species is suggested.
authors.affiliation: Gamaleya Institute of Epidemiology and Microbiology, USSR Academy of Medical Sciences, Moscow.
authors.forename: M N
authors.initials: MN
authors.lastname: Rozinov
authors.forename: A A
authors.initials: AA
authors.lastname: Dain
authors.forename: Y L
authors.initials: YL
authors.lastname: Shumakov
authors.forename: I A
authors.initials: IA
authors.lastname: Lapaeva
authors.forename: T A
authors.initials: TA
authors.lastname: Holzmayer
mesh_terms: D015139:Blotting, Southern; D001884:Bordetella / Q000235:genetics*; D001886:Bordetella pertussis / Q000235:genetics*; D003001:Cloning, Molecular; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D037342:Pertussis Toxin; D010957:Plasmids; D010566:Virulence Factors, Bordetella / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific