Possible tumour-initiating and -promoting activities of 3-diazo-N-nitrosobamethan in rat stomach mucosa.
3-Diazo-N-nitrosobamethan (DNB), a mutagen produced by nitrite treatment of bamethan, a cardiovascular drug, induced unscheduled DNA synthesis in the pyloric mucosa of the stomach of male F344 rats 2 h after its administration by gastric tube at doses of 75 to 225 mg/kg body wt. DNB at a dose of 225 mg/kg body wt induced up to a 13-fold increase in replicative DNA synthesis with a maximum at 16 h after its administration. Moreover at doses of 75 to 225 mg/kg body wt, it induced up to a 12-fold increase in ornithine decarboxylase activity with a maximum after 16 h. The present results suggest that DNB has possible tumour-initiating and -promoting activities in the pyloric mucosa of the rat stomach. However, the possible risks to patients posed by clinical use of bamethan cannot be evaluated until further work is completed.
Furihata
C
C
Department of Molecular Oncology, University of Tokyo, Japan.
Yamakoshi
A
A
Matsushima
T
T
Kato
T
T
Kikugawa
K
K
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004261:DNA Replication / Q000187:drug effects; D004790:Enzyme Induction; D004983:Ethanolamines / Q000031:analogs & derivatives* / Q000633:toxicity; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis; D010627:Phenethylamines; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D004983:Ethanolamines; D009153:Mutagens; D010627:Phenethylamines; C051613:3-diazo-N-nitrosobamethan; D009955:Ornithine Decarboxylase