Experimental liver metastasis. Implications of clonal proclivity and organ specificity.
"Spontaneous" lung metastases develop in over 50% of the animals bearing subcutaneous isografts of WB-2054, a rat colon carcinoma. A metastatic variant has been developed by "Fidler" type in vivo selection, yielding 100% lung metastasis. In a five-week assay to test the organ specificity of this lung metastatic variant, however, "experimental" liver and lung metastases could be induced in 100% and 60% of animals on portal venous and intravenous injections, respectively. The results demonstrate selection of a metastatic variant from heterogeneous primary tumor, and suggest at least two interacting mechanisms: (1) mechanical (the anatomy of the blood-borne metastatic pathways) and (2) biologic (factors intrinsic to primary tumor subpopulations that can be selected for metastatic proclivity). In addition, liver metastases were successfully established from colon tumors induced by cecal wall injection of tumor cells. Such a spontaneous liver metastasis model will be useful to study the specific mechanisms involved during metastasis of colon cancer to the liver.
Ravikumar
T S
TS
Department of Surgery, New England Deaconess Hospital/Harvard Medical School, Boston, MA.
D'Emilia
J
J
Cocchiaro
C
C
Wolf
B
B
King
V
V
Steele
G
G
D019813:1,2-Dimethylhydrazine; D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology / Q000556:secondary; D000818:Animals; D002430:Cecal Neoplasms / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced; D004127:Dimethylhydrazines; D008099:Liver / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000556:secondary*; D008168:Lung / Q000473:pathology; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D008297:Male; D009368:Neoplasm Transplantation; D009928:Organ Specificity; D051381:Rats; D011914:Rats, Inbred BN; D011920:Rats, Inbred WF
D004127:Dimethylhydrazines; D019813:1,2-Dimethylhydrazine