Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile.
The physicochemical properties of apparently acid-labile IFN-alpha from patients with SLE have been studied. The antigenicity, apparent molecular size, and isoelectric point of SLE IFN-alpha are indistinguishable from those of conventional, previously characterized, acid-stable subspecies of IFN-alpha. However, after partial purification by anion-exchange chromatography, SLE IFN-alpha no longer exhibits acid lability, suggesting that other plasma factor(s) are responsible for the acid lability of SLE IFN-alpha. Addition of SLE plasma, but not normal plasma, to conventional acid-stable IFN-alpha renders the exogenous IFN-alpha acid labile. Preliminary results demonstrate that an acid-dependent IFN-inactivating activity can be partially purified from SLE plasma by anion-exchange chromatography.
Yee
A M
AM
Department of Microbiology, Sackler Institute of Graduate Biomedical Sciences, New York, New York.
Buyon
J P
JP
Yip
Y K
YK
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007370:Interferon Type I / Q000097:blood*; D007526:Isoelectric Point; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008970:Molecular Weight; D009500:Neutralization Tests
D007370:Interferon Type I