title: Coordinate accumulation of troponin subunits in chicken breast muscle.
abstract: The accumulation of troponin subunits in developing chicken breast muscle was determined by two-dimensional gel electrophoresis and an image analyzing system. Many troponin T isoforms, including those hidden behind creatine kinase, were detected on the two-dimensional pattern by the addition of 6 M urea in the second dimension. These troponin T isoforms were classified into four types by developmental order, isoelectric point, and molecular weight: leg-muscle type (L), neonatal breast-muscle type (BN), young chicken breast-muscle type (BC), and adult breast-muscle type (BA). The L-, BN-, and BC-type troponin Ts were transiently expressed at specific developmental stages. Quantitative analysis of two-dimensional patterns of troponin subunits including troponin I and troponin C showed moderate coordination in accumulation among the three subunits throughout postnatal development, when the total amount of all isoforms of troponin T was taken into account.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Imai
authors.forename: T
authors.initials: T
authors.lastname: Hirabayashi
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D008970:Molecular Weight; D024510:Muscle Development; D009132:Muscles / Q000196:embryology / Q000378:metabolism; D014336:Troponin / Q000378:metabolism*; D020107:Troponin T
chemical_list: D006146:Guanidines; D046911:Macromolecular Substances; D014336:Troponin; D020107:Troponin T; D019791:Guanidine