title: Heat inactivation of human immunodeficiency virus in solutions of antithrombin III.
abstract: Human immunodeficiency virus (HIV), isolated from cultures of infected peripheral blood lymphocytes, was added to solutions of highly purified antithrombin III (AT-III), which was stabilized with 1 M citrate and 17 percent sucrose. The efficiency of heat inactivation of HIV in AT-III at 60 degrees C was compared with that of HIV in culture medium and followed for periods from 0 to at least 10 hours. The virus added, titer 10(5) by ID50, was inactivated as rapidly (less than 30 minutes) and efficiently (completely) in the stabilized AT-III as in the culture medium. Virus was determined both by direct measurement of the HIV-related reverse transcriptase activity and by quantitation of virus infectivity by ID50 assay.
authors.affiliation: KabiVitrum AB, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Einarsson
authors.forename: L
authors.initials: L
authors.lastname: Perenius
authors.forename: J S
authors.initials: JS
authors.lastname: McDougal
authors.forename: S
authors.initials: S
authors.lastname: Cort
mesh_terms: D000990:Antithrombin III; D005069:Evaluation Studies as Topic; D006678:HIV / Q000254:growth & development* / Q000302:isolation & purification; D006358:Hot Temperature; D006801:Humans; D012996:Solutions; D013696:Temperature; D013997:Time Factors; D014775:Virus Activation
chemical_list: D012996:Solutions; D000990:Antithrombin III