Purification of a growth factor related to platelet-derived growth factor and a type beta transforming growth factor secreted by mouse neuroblastoma cells. A general strategy for the purification of basic polypeptide growth factors.
A general strategy was developed for the purification of basic polypeptide growth factors. This method is a combination of gel filtration, weak-cation-exchange h.p.l.c. and reverse-phase h.p.l.c., separating the proteins according to size, charge and hydrophobicity respectively. All steps are carried out at low pH with exclusively volatile acidic buffer solutions. The sterile conditions and easy removal of salt by freeze-drying facilitate the detection of the growth factors by biological assays. By using this method, homogeneous preparations of two basic growth factors were purified in high yield from mouse-neuroblastoma-Neuro-2A-cell-conditioned medium. It is shown that these purified factors are biochemically and immunologically related to platelet-derived growth factor and type beta transforming growth factor from human platelets.
Van den Eijnden-Van Raaij
A J
AJ
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht, The Netherlands.
Koornneef
I
I
Van Oostwaard
T M
TM
Feyen
A
A
Kruijer
W
W
De Laat
S W
SW
Van Zoelen
E J
EJ
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005786:Gene Expression Regulation; D006133:Growth Substances / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D009447:Neuroblastoma / Q000032:analysis* / Q000235:genetics; D010455:Peptides / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D015291:Transforming Growth Factors / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D006133:Growth Substances; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D015291:Transforming Growth Factors