The isoforms of C protein and their distribution in mammalian skeletal muscle.
A monoclonal antibody that is specific for the slow skeletal muscle isoform of C protein of rabbit muscle has been prepared by immunizing mice with a crude preparation of human myosin. It reacted with the X protein fraction of rabbit skeletal muscle and stained all type I cells in this tissue. It also stained a fraction of the type II cells with varying intensities. The type II cells staining with antibody to slow C protein also stained with a polyclonal antibody prepared against rabbit fast muscle C protein. The type II cells not staining with antibody to slow C protein stained strongly with antibody to fast C protein. In the human skeletal muscle antibody to slow C protein stained all cells whereas antibody to fast C stained only type II cells. It is concluded that the distribution of the isoforms of C protein in adult vertebrate skeletal muscle is more complex than is the case with proteins such as components of the troponin complex.
Dhoot
G K
GK
Hales
M C
MC
Grail
B M
BM
Perry
S V
SV
D000251:Adenosine Triphosphatases / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000032:analysis* / Q000201:enzymology; D009218:Myosins / Q000032:analysis; D011817:Rabbits; D013045:Species Specificity; D013329:Structure-Activity Relationship; D014018:Tissue Distribution; D000099163:Myosin Binding Protein C
D000251:Adenosine Triphosphatases; D000596:Amino Acids; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D009124:Muscle Proteins; D009218:Myosins; D000099163:Myosin Binding Protein C