Cytotoxic activity of crude extracts of Bacteroides gingivalis.
The cytotoxic activities of culture supernates, crude cell extracts and cell-wall materials of Bacteroides gingivalis were investigated in vitro. Each component was cytotoxic to Vero cells and, to a lesser extent, Wi 38 cells. The cytotoxic agents had similar effects on the cell lines to butyric acid, propionic acid and a partially-purified trypsin-like protein extracted from a clinical isolate of B. gingivalis; the effects were eliminated by heat. Cytotoxic materials obtained from young cultures were more susceptible to heat than those from older cultures. The heart-labile substance inside and outside the bacterial cell in young cultures of B. gingivalis may contribute to its overall cytotoxic activity.
Eke
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PI
Department of Medical Microbiology, College of Medicine, Lagos, Nigeria.
Laughon
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BE
Rotimi
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D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology; D001439:Bacteroides / Q000502:physiology* / Q000648:ultrastructure; D002460:Cell Line; D002470:Cell Survival; D002473:Cell Wall / Q000502:physiology; D003603:Cytotoxins / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology; D005347:Fibroblasts; D006801:Humans; D013053:Spectrophotometry; D014709:Vero Cells
D001426:Bacterial Proteins; D003603:Cytotoxins; D005227:Fatty Acids