Detection of picogram levels of sufentanil by capillary gas chromatography.
Sufentanil and its two primary metabolites, N-(4-[methoxymethyl]-4-piperidinyl)-N-phenyl propanamide (MPPP), and N-(4[hydroxymethyl]-1-[2-thienylethyl]-4-piperidinyl)-N-phenyl propanamide (desmethyl sufentanil), were detected by capillary gas chromatography with a nitrogen-phosphorous detector. The detection limit for sufentanil and its metabolites is 30-50 pg/ml with minimal interfering substances in the chromatograms. This method allowed for the detection of serum sufentanil in the terminal elimination phase of sufentanil in a patient receiving 1.5 micrograms/kg and will allow for studies to determine the pharmacokinetics and metabolism of sufentanil in a wide variety of patient groups now receiving this agent.
Weldon
S T
ST
Perry
D F
DF
Cork
R C
RC
Gandolfi
A J
AJ
D000328:Adult; D001711:Biotransformation; D002849:Chromatography, Gas; D005283:Fentanyl / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008297:Male; D008832:Microchemistry; D017409:Sufentanil / Q000031:analogs & derivatives
C047304:desmethylsufentanil; D017409:Sufentanil; C047303:noralfentanil; D005283:Fentanyl