Estrogen-responsive element of the human pS2 gene is an imperfectly palindromic sequence.
Using chimeric recombinants transfected into HeLa cells and a transient expression assay, we demonstrate that the 5'-flanking region of the pS2 gene from position -428 to position -324 exhibits both constitutive and estrogen-inducible enhancer activity. The estrogen-inducible activity, but not the constitutive activity, was inhibited by antiestrogens. ICI 164,384 behaved as a pure antagonist, whereas hydroxytamoxifen was a partial agonist-antagonist. The estrogen-responsive element of the pS2 gene has been narrowed down by site-directed deletion mutagenesis to a 13-base-pair (position -405 to position -393) imperfectly palindromic sequence, which in isolation can confer estrogen inducibility to the heterologous rabbit beta-globin gene promoter. On the other hand, the sequences responsible for the constitutive enhancer activity are spread over the entire region.
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Unité 184 de Biologie Moléculaire et de Génie Génétique de l'Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine, Strasbourg, France.
Nunez
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AM
Chambon
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D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D004967:Estrogens / Q000494:pharmacology*; D005796:Genes / Q000187:drug effects*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011506:Proteins; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins
D004967:Estrogens; D009363:Neoplasm Proteins; D011506:Proteins; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins