Hypomethylation of ornithine decarboxylase gene and erb-A1 oncogene in human chronic lymphatic leukemia.
The methylation state of CCGG sites in and around the human ornithine decarboxylase gene, oncogenes c-myc and erb-A1, and actin genes were determined in human malignant leucocytes from patients with acute and chronic myeloid leukemia, chronic lymphatic leukemia, polycythemia vera, and multiple myeloma by means of isoschizomeric restriction endonuclease analysis. When compared with DNA from leucocytes of healthy controls, the ornithine decarboxylase and erb-A1 genes were substantially hypomethylated in all samples obtained from patients with chronic lymphatic leukemia. Hypomethylation of genes, particularly growth-related sequences, might be a crucial fact in the malignant transformation of human leucocytes. Its relatively simple detection from blood samples may prove clinically applicable in monitoring patients with chronic lymphatic leukemia.
Lipsanen
V
V
Department of Biochemistry, University of Helsinki, Finland.
Leinonen
P
P
Alhonen
L
L
Jänne
J
J
D001402:B-Lymphocytes / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005786:Gene Expression Regulation; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008745:Methylation; D009363:Neoplasm Proteins / Q000235:genetics; D018774:Oncogene Proteins v-erbA; D009955:Ornithine Decarboxylase / Q000235:genetics*; D015183:Restriction Mapping; D012191:Retroviridae Proteins / Q000235:genetics*
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D018774:Oncogene Proteins v-erbA; D012191:Retroviridae Proteins; D009955:Ornithine Decarboxylase