Protective effect of salicylate against 2,4-dinitrophenol-induced protein thiol loss in the small intestine of rats.
The effect of the content of protein thiols and non-protein thiols in the tissue of the rat small intestinal loop on the absorption of cefmetazole has been investigated. 2,4-Dinitrophenol (DNP), at a concentration of 500 microM, caused rapid protein thiol loss, followed by non-protein thiol loss, along with a decrease of cefmetazole absorption by the intestine. The coadministration of 75 mM salicylate with DNP inhibited the effect of DNP on protein thiols and cefmetazole absorption but not on non-protein thiol loss by DNP.
Nishihata
T
T
Pharmaceutical Chemistry Department, University of Kansas, Lawrence 66046.
Suzuka
T
T
Yata
N
N
Sakai
K
K
D019297:2,4-Dinitrophenol; D000818:Animals; D015311:Cefmetazole / Q000493:pharmacokinetics*; D004140:Dinitrophenols / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012459:Salicylates / Q000494:pharmacology*; D020156:Salicylic Acid; D013438:Sulfhydryl Compounds / Q000378:metabolism
D004140:Dinitrophenols; D011506:Proteins; D012459:Salicylates; D013438:Sulfhydryl Compounds; D015311:Cefmetazole; D020156:Salicylic Acid; D019297:2,4-Dinitrophenol