Genetic transformation of mouse cultured cells with the help of high-velocity mechanical DNA injection.
NIH 3T3 mouse cells were transfected by the plasmid pSV3neo (G418-resistant) with the help of high-velocity mechanical DNA injection based on the principle of bombarding cells with tungsten particles covered with the DNA. Stable transformants were obtained. Dot-hybridization and Southern analysis revealed the integration into the genome of 5-20 copies per cell of original plasmid DNA. The plasmid DNA was shown to have tandem organization.
Zelenin
A V
AV
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Titomirov
A V
AV
Kolesnikov
V A
VA
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D051379:Mice; D008845:Microinjections; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014162:Transfection / Q000379:methods*; D014170:Transformation, Genetic; D014414:Tungsten
D004247:DNA; D015246:Deoxyribonuclease EcoRI; D014414:Tungsten