Characterization and regulation by protein kinase C of renal glomerular atrial natriuretic peptide receptor-coupled guanylate cyclase.
The nature and regulation of atrial natriuretic peptide (ANP)-sensitive guanylate cyclase in rat renal glomerular membranes was examined. By affinity crosslinking techniques, three bands with apparent molecular masses of 180, 130 and 64 kDa were specifically labeled with [125I]ANP. A specific antibody to the 180 kDa membrane guanylate cyclase of rat adrenocortical carcinoma recognized a 180 kDa band on Western blot analysis of solubilized, GTP-affinity purified glomerular membrane proteins. The same antibody completely inhibited ANP-stimulated guanylate cyclase activity in glomerular membrane fractions. Partially purified protein kinase C inhibited ANP-stimulated guanylate cyclase activity in glomerular membrane fractions. It is concluded that a 180 kDa ANP-sensitive guanylate cyclase is present in glomerular membranes, and that this enzyme is inhibited directly by protein kinase C.
Ballermann
B J
BJ
Renal Division, Brigham and Women's Hospital, Boston, MA.
Marala
R B
RB
Sharma
R K
RK
D000345:Affinity Labels; D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000502:physiology; D003432:Cross-Linking Reagents; D006162:Guanylate Cyclase / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000201:enzymology*; D008970:Molecular Weight; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000502:physiology*
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D011956:Receptors, Cell Surface; D011493:Protein Kinase C; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor