Adrenergic receptors on cerebral microvessels: pericyte contribution.
R224-R230, 1989.--[125I]iodocyanopindolol ([125I]ICYP) and [3H]rauwolscine were used to quantitate, respectively, the beta- and alpha 2-adrenergic receptors in freshly isolated bovine cerebral microvessels and in pericyte cultures derived from these microvessels. Morphological and immunocytochemical criteria distinguished the pericytes from endothelial cells. Competitive binding studies established the specificity of the radioligand binding. The maximal number of binding sites (Bmax) for [125I]ICYP in the pericytes constituted only 8% of that in the microvessels (3.5 +/- 1.3 vs. 44.4 +/- 6.6 fmol/mg protein). In contrast, the Bmax for [3H]rauwolscine in the pericytes was 50% of that in the microvessels (55.4 +/- 11.8 vs. 111.1 +/- 9.5 fmol/mg protein). The dissociation constants for both [125I]ICYP and [3H]rauwolscine were similar in the two preparations. No alpha 1-adrenergic receptors, as defined by the specific binding of [3H]prazosin, were identified either in the pericytes or microvessels. Overall, our results suggest that pericytes contribute minimally to the total beta-adrenoceptor number of cerebral microvessels, and thus the beta-adrenoceptors must be located predominantly on endothelial cells. However, the contribution of pericytes to the total alpha 2-adrenoceptor number of the microvessels may be substantial.
Elfont
R M
RM
Department of Neurobiology, University of Rochester Medical Center, New York 14642.
Sundaresan
P R
PR
Sladek
C D
CD
D000818:Animals; D001667:Binding, Competitive; D001812:Blood-Brain Barrier; D002417:Cattle; D002560:Cerebrovascular Circulation; D020120:Iodocyanopindolol; D007700:Kinetics; D008833:Microcirculation; D008854:Microscopy, Electron; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011224:Prazosin / Q000378:metabolism; D015016:Yohimbine / Q000378:metabolism
D015016:Yohimbine; D020120:Iodocyanopindolol; D010869:Pindolol; D011224:Prazosin