Mutational analysis of primosome assembly sites. Evidence for alternative DNA structures.
Primosome assembly sites are complex DNA structures that share common functions (they elicit the DNA-dependent ATPase of replication factor Y from Escherichia coli and serve as origins of complementary strand DNA synthesis), but display little sequence homology. In order to ascertain a common basis for factor Y-DNA recognition, a primosome assembly site and its mutated derivatives have been functionally and structurally analyzed. Under conditions in which they lose the capacity to function as ATPase effectors these DNA templates have been (i) assayed for their ability to bind factor Y, and (ii) probed, with pancreatic DNase, for structural alterations. In this ATPase-inactivating environment (suboptimal concentrations of MgCl2 and NaCl, and high levels of the E. coli single-stranded DNA binding protein), factor Y does not bind to its cognate DNA and the DNase cleavage pattern characteristic of this site is perceptibly changed: compared to the DNase digest obtained under activating conditions, cleavage is notably decreased in the 5' half of the site and enhanced at the 3' end. The results of this study strongly indicate that the structure of the primosome assembly site under analysis consists of two hairpins which interact with each other. When the sites of pancreatic DNase attack are plotted on the proposed double hairpin structure, the 5' cleavage sites all map to one duplex while the 3' sites map to the other. The observation that, under factor Y ATPase-activating conditions, the 3' hairpin is largely refractory to the action of pancreatic DNase indicates that tertiary interactions between the two duplexes render a portion of the DNA structure inaccessible to the nuclease.
Greenbaum
J H
JH
Marians
K J
KJ
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004265:DNA Helicases; D004269:DNA, Bacterial / Q000235:genetics*; D003851:Deoxyribonucleases; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011485:Protein Binding; D013698:Templates, Genetic
D004269:DNA, Bacterial; D003851:Deoxyribonucleases; D000251:Adenosine Triphosphatases; D004265:DNA Helicases