State of hepatitis B virus DNA in hepatocytes of patients with noncarcinomatous liver disease. Its special relationship with necroinflammatory activity and the stage of disease.
By Southern blot hybridization, the state of hepatitis B virus (HBV) in the liver was investigated by utilizing needle biopsy specimens of 59 patients with hepatitis B surface antigen-positive noncarcinomatous liver disease. The tissue HBV DNA revealed a replicative form in 23 patients with chronic active hepatitis (48%) and an integrated form in 23 (48%) of these 48 patients. A liver with a replicative form was more frequently associated with hepatitis Be antigen (HBeAg), serum HBV DNA, and expression of hepatitis B core antigen than one without it. Integration of HBV DNA was more common in the anti-hepatitis Be phase or in the absence of necroinflammatory activity, and its frequency of occurrence roughly paralleled progression of liver disease: early, 21%; advanced, 56%; and cirrhotic stage, 61%.
Tozuka
S
S
Department of Pathology, Nihon University School of Medicine, Tokyo, Japan.
Uchida
T
T
Suzuki
K
K
Esumi
M
M
Shikata
T
T
D000328:Adult; D015139:Blotting, Southern; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006509:Hepatitis B / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000502:physiology; D006521:Hepatitis, Chronic / Q000382:microbiology; D006801:Humans; D007249:Inflammation; D008099:Liver / Q000382:microbiology* / Q000473:pathology; D008103:Liver Cirrhosis / Q000209:etiology / Q000382:microbiology; D008297:Male; D009336:Necrosis; D014779:Virus Replication
D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens