Gene on short arm of human X chromosome complements murine tsA1S9 DNA synthesis mutation.
We have created somatic cell hybrids between the temperature-sensitive mouse cell line tsA1S9 and human cell lines in order to localize the human gene (A1S9T) complementing the cell cycle defect of the murine line. Segregation of the human X chromosome is completely concordant with growth at the nonpermissive temperature. Hybrids retaining the X chromosome are temperature-resistant, whereas those without a human X are temperature-sensitive. Further hybrids made using human cell lines with X-autosome translocations indicate that the A1S9T gene is located on the short arm of the human X chromosome.
Brown
C J
CJ
Department of Medical Genetics, University of Toronto, Canada.
Powers
V E
VE
Munroe
D L
DL
Sheinin
R
R
Willard
H F
HF
D000818:Animals; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D005816:Genetic Complementation Test; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009154:Mutation; D013696:Temperature; D014960:X Chromosome
D004247:DNA