Detection of 6-thioguanine resistance in human peripheral blood lymphocytes (PBL) of industrial workers and lung cancer patients.
Human peripheral blood lymphocytes (PBL) were selected for 6-thioguanine (6-TG) resistance in short-term (42-h) cultures in 110 high-cancer-risk industrial workers, 131 primary lung cancer patients and 96 low-risk controls. The lymphocytes were cultured and stimulated by phytohemagglutinin (PHA). A labeling index (LI) was scored using light microscope autoradiography, based on the lymphocyte's ability to incorporate tritiated thymidine with or without selective agent 6-TG. The number of 6-TG-resistant cells increased in the high-occupational-cancer-risk group of vinyl chloride- and mixed organic industrial dust (MOID)-exposed workers as well as in the primary lung cancer patients. The results were compared with the low-occupational-cancer-risk groups and with samples taken from the 70 healthy individuals and 26 hospitalized, non-cancerous controls. In both risk-exposed groups the frequency of 6-TG-resistant lymphocytes was significantly higher (p less than 0.01) than in the controls. These results suggest that the original Strauss and Albertini (1977, 1979) method can be used to study qualitative risk assessment in carcinogen- or mutagen-exposed occupational groups.
Tompa
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A
National Institute of Occupational Health, Budapest, Hungary.
Sapi
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E
D002273:Carcinogens / Q000633:toxicity*; D004351:Drug Resistance; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency / Q000235:genetics*; D066298:In Vitro Techniques; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*; D009784:Occupational Diseases / Q000235:genetics*; D013866:Thioguanine / Q000633:toxicity
D002273:Carcinogens; D009153:Mutagens; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine