title: Is there any correlation between platelet aggregation, plasma lipoproteins, apoproteins and membrane fluidity of human blood platelets?
abstract: Fluorescence anisotropy (which is inversely related to membrane fluidity) of gel filtered platelets of 18 normolipemic subjects (20-26 years) was measured after incubation with three different fluorescent probes (DPH, TMA-DPH, and 6-As). These values were correlated to both platelet aggregation parameters after stimulation with ADP (4 microM), epinephrine (10 microM) or collagen (2 micrograms/ml PRP) and to plasma lipids, lipoproteins and apoproteins. Fluorescence anisotropy values after DPH-labeling of platelets were only negatively correlated to TG (p less than 0.05). No correlation was found between fluorescence anisotropy values of DPH, TMA-DPH and 6-As to LDL-C, Lp(a), HDL-C and HDL3-C (p less than 0.01). However, fluorescence anisotropy values of DPH and TMA-DPH were negatively correlated to apoproteins A2 and B (p less than 0.05). No correlations were found between fluorescence anisotropy after DPH labeling and different aggregation parameters. TMA-DPH and 6-As fluorescence anisotropy values are correlated to epinephrine induced stimulation (p less than 0.01).
authors.affiliation: Institute of Medical Biochemistry, University of Graz, Austria.
authors.forename: E
authors.initials: E
authors.lastname: Malle
authors.forename: A
authors.initials: A
authors.lastname: Gries
authors.forename: G M
authors.initials: GM
authors.lastname: Kostner
authors.forename: K
authors.initials: K
authors.lastname: Pfeiffer
authors.forename: J
authors.initials: J
authors.lastname: Nimpf
authors.forename: A
authors.initials: A
authors.lastname: Hermetter
mesh_terms: D000328:Adult; D001059:Apoproteins / Q000097:blood*; D005454:Fluorescence Polarization; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008560:Membrane Fluidity; D010974:Platelet Aggregation
chemical_list: D001059:Apoproteins; D008074:Lipoproteins