Impaired intracellular transport of class I MHC antigens as a possible means for adenoviruses to evade immune surveillance.
Early region E3 of adenovirus-2 encodes a glycoprotein, E19, that associates with class I MHC antigens. The interaction occurs concomitantly with abrogated terminal glycosylation of class I antigen heavy chains, as shown by pulse-chase and Endo H digestion experiments. Also the viral protein remains in the high mannose form throughout the chase period. These results do not reflect a general impairment of terminal glycosylation of membrane proteins. Immunofluorescence analyses demonstrated that in virally infected cells E19 and class I antigens accumulate in the perinuclear region. Class I antigens gradually disappear from the cell surface. A diminished cell surface expression of class I antigens may be a means by which adenoviruses evade immune surveillance.
Andersson
M
M
Pääbo
S
S
Nilsson
T
T
Peterson
P A
PA
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000276:immunology* / Q000502:physiology; D001692:Biological Transport; D002462:Cell Membrane / Q000276:immunology*; D006680:HLA Antigens; D006367:HeLa Cells; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008565:Membrane Proteins / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D014162:Transfection; D014764:Viral Proteins / Q000378:metabolism*
D017456:Adenovirus Early Proteins; D006680:HLA Antigens; D008565:Membrane Proteins; D009856:Oncogene Proteins, Viral; D014764:Viral Proteins; D006596:Hexosaminidases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase