Lack of effect of pertussis toxin on TNF-alpha-induced formation of reactive oxygen intermediates by human neutrophils.
When human PMN were plated on fetal calf serum-coated polystyrene surfaces, addition of TNF-alpha, FMLP or PMA elicited adhesion and H2O2 formation. These effects of TNF-alpha and FMLP, but not of PMA, were impaired by removal of extracellular Ca2+. In addition, H2O2 formation induced by FMLP but not by TNF-alpha or PMA was inhibited by prior treatment with pertussis toxin (250-500 ng/ml). Thus, although the sequelae of TNF-alpha-receptor interaction on human PMN remain to be characterized in detail, they do not involve a pertussis toxin-sensitive GTP-binding protein.
Meurer
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Merck Sharp & Dohme Research Laboratories, Department of Biochemistry and Molecular Pathology, Rahway, New Jersey 07065.
MacIntyre
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D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D005312:Fetal Blood; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000096:biosynthesis; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D010101:Oxygen Consumption / Q000187:drug effects; D037342:Pertussis Toxin; D013755:Tetradecanoylphorbol Acetate; D014409:Tumor Necrosis Factor-alpha; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D005609:Free Radicals; D014409:Tumor Necrosis Factor-alpha; D010566:Virulence Factors, Bordetella; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide; D037342:Pertussis Toxin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium