title: Immunohistochemical localization of glutamate decarboxylase in the rat oviduct and ovary: further evidence for non-neural GABA systems.
abstract: The distribution of L-glutamate decarboxylase (GAD), a major biosynthetic enzyme for gamma-aminobutyric acid (GABA), was examined in the oviduct and ovary of the rat by means of an immunohistochemical technique. The polyclonal antiserum raised against brain GAD showed specific immunoreaction in some non-neuronal elements of the sex organs. In the oviduct, the inner layer of the mucosa was predominantly labelled. The selective distribution of GAD immunoreactivity in epithelial cells of the oviduct is consistent with former findings for GABA-like immunoreactivity in the same organ, indicating that the GAD-catalyzed reaction may be a major biosynthetic pathway for GABA even in these cells. In the ovary, vacuole-like formations within the follicular fluid and oocytes showed intense, specific staining. The occurrence of GAD immunoreactivity inside developing ovarian follicles including the oocyte may suggest a role for GABA related to follicular development and certain functions concerning the ovum.
authors.affiliation: Department of Anatomy, Georg August University, Göttingen, Federal Republic of Germany.
authors.forename: S L
authors.initials: SL
authors.lastname: Erdö
authors.forename: F
authors.initials: F
authors.lastname: Joo
authors.forename: J R
authors.initials: JR
authors.lastname: Wolff
mesh_terms: D000818:Animals; D005187:Fallopian Tubes / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D005260:Female; D005968:Glutamate Decarboxylase / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D010053:Ovary / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000276:immunology / Q000378:metabolism
chemical_list: D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase