Immunocytochemical demonstration of Langerhans' cells in smears and pellets of exfoliated cells from human exocervices.
Immunocytochemical staining with OKT6 monoclonal antibody and S-100 protein antiserum was used to reveal Langerhans' cells in smears and/or pellets of exfoliated cells from uterine cervices (normal and with squamous carcinoma). Immunoreactive Langerhans' cells were found exclusively in smears and pellets of cervices with squamous carcinoma. Langerhans' cells, which appear as rounded cells, showed a peripheral ring of intense fluorescence with OKT6 antibody whereas the entire cell stained with S-100 protein antiserum. The presence of Langerhans' cells among cells exfoliated from exocervical squamous carcinoma can be explained by the increased density of these cells in tissue with neoplastic changes.
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Department of Histology and Pathology, Faculty of Medicine, Southern University of Chile, Valdivia.
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Caorsi
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D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000473:pathology; D002584:Cervix Uteri / Q000166:cytology*; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000166:cytology*; D009418:S100 Proteins / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology
D000911:Antibodies, Monoclonal; D009418:S100 Proteins