title: Fluorimetric studies of protein kinase C interactions with phospholipids.
abstract: Dansyl-phosphatidylserine (D-PS) was used as a fluorescence probe to study interactions between protein kinase C (PKC) with phospholipid vesicles. It was found that D-PS fluorescence (520 nm) was enhanced by PKC (excited at 285 nm). The fluorescence energy transfer, indicative of a close association of PKC with D-PS vesicles, was differentially modulated by various phospholipids, depending upon their effects on PKC activation state and the manners in which they were present. PKC inhibitors (e.g. polymyxin B and ether lipids) potently inhibited the PKC-enhanced D-PS fluorescence. It is suggested that certain spatial arrangements between PKC and its phospholipid cofactor are essential for the enzyme activation and that D-PS would be a useful probe to study fluorimetrically such interactions.
authors.affiliation: Department of Pharmacology, Emory University, Atlanta, Georgia 30322.
authors.forename: J M
authors.initials: JM
authors.lastname: Rodriguez-Paris
authors.forename: M
authors.initials: M
authors.lastname: Shoji
authors.forename: S
authors.initials: S
authors.lastname: Yeola
authors.forename: D
authors.initials: D
authors.lastname: Liotta
authors.forename: W R
authors.initials: WR
authors.lastname: Vogler
authors.forename: J F
authors.initials: JF
authors.lastname: Kuo
mesh_terms: D000818:Animals; D002308:Cardiolipins; D003619:Dansyl Compounds; D004735:Energy Transfer; D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; D011493:Protein Kinase C; D051381:Rats; D013050:Spectrometry, Fluorescence
chemical_list: D002308:Cardiolipins; D003619:Dansyl Compounds; D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; D011493:Protein Kinase C