title: The ultrastructure of upstream and downstream regions of an active Balbiani ring gene.
abstract: When active, the 37 kb Balbiani ring genes are known to form transcription loops with an almost fully extended chromatin axis. Here we examine the upstream and downstream regions of such transcription loops by electron microscopy. We demonstrate that a loop starts and ends in tightly packed chromatin; the two anchoring sites are clearly separated from each other in space. The upstream, nontranscribed region consists of a thin, extended, apparently flexible and nucleosome-free fiber corresponding to about 0.5 kb DNA. The downstream, nontranscribed region appears as a 200 nm long nucleofilament loosely coiled into a short, thick chromatin fiber and estimated to contain about 3 kb DNA.
authors.affiliation: Department of Molecular Genetics, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Ericsson
authors.forename: H
authors.initials: H
authors.lastname: Mehlin
authors.forename: B
authors.initials: B
authors.lastname: Björkroth
authors.forename: M M
authors.initials: MM
authors.lastname: Lamb
authors.forename: B
authors.initials: B
authors.lastname: Daneholt
mesh_terms: D000818:Animals; D002683:Chironomidae / Q000235:genetics / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure*; D004175:Diptera / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin