title: Glucose, glutamine and ketone body utilisation by resting and concanavalin A activated rat splenic lymphocytes.
abstract: The utilisation of glucose, glutamine, acetoacetate and D-3-hydroxybutyrate were investigated over 72 h of incubation of rat splenic lymphocytes, with and without concanavalin A. Lymphocytes consumed both ketone bodies; acetoacetate was consumed preferentially. The ketone bodies reduced glucose consumption by 30-50%, but had little effect on lactate production. Glutamine uptake was concentration dependent up to 4 mM, and consumption was increased in the presence of concanavalin. Glutamine stimulated glucose consumption and lactate production in both resting and activated cells. Complete oxidation contributed 65% of glucose-derived ATP, but less than 40% of glutamine-derived ATP. Glutamine metabolism makes only a minor contribution to lymphocyte ATP generation.
authors.affiliation: Department of Biochemistry, Royal Holloway and Bedford New College, University of London, Egham, Surrey, U.K.
authors.forename: A M
authors.initials: AM
authors.lastname: O'Rourke
authors.forename: C C
authors.initials: CC
authors.lastname: Rider
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism* / Q000494:pharmacology; D007657:Ketone Bodies / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000378:metabolism
chemical_list: D007657:Ketone Bodies; D007773:Lactates; D005973:Glutamine; D003208:Concanavalin A; D000255:Adenosine Triphosphate; D005947:Glucose