Recovery of motile sperm using the migration-sedimentation technique in an in-vitro fertilization-embryo transfer programme.
Sperm washing techniques, based on the swim-up principle used before inseminating the human oocyte in in-vitro fertilization and embryo transfer programmes (IVF-ET), usually require prior centrifugation which causes damage to the sperm cell. A technique is described for separating sperm at laboratory temperature based on sperm migration--sedimentation principles, using two concentric tubes and recovering 70-90% forward-moving cells. A group of 17 patients is presented who were managed with this method. The results were 85% fertilization rate, 4% polyspermia and six clinical pregnancies.
Lucena
E
E
Centro Colombiano de Fértilidad y Esterilidad, Bogotá.
Lucena
C
C
Gómez
M
M
Ortiz
J A
JA
Ruiz
J
J
Arango
A
A
Diaz
C
C
Beuerman
C
C
D002469:Cell Separation; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*