title: Ganglioside mapping of a human medulloblastoma xenograft.
abstract: The ganglioside patterns of medulloblastomas have never been established; in this study we report the ganglioside profile of the human medulloblastoma cell line TE-671 grown as a xenograft in nude mice. Gangliosides were isolated and structurally analyzed by fast atom bombardment mass spectometry following permethylation. Identification of individual gangliosides was also performed by immunostaining of high-performance thin-layer chromatography-separated bands. Total ganglioside levels of 0.20 mumol/g of tissue were obtained, consistent with those reported for human glioma cell lines grown as xenografts; predominant monosialogangliosides of TE-671 xenografts were II3-alpha-NeuAc-LacCer (GM3) and II3-alpha-NeuAc-GgOse3Cer (GM2) but there were also relatively large proportions of IV3-alpha-NeuAc-LcOse4Cer (3'-isoLM1), IV3-alpha-NeuAc-nLcOse4Cer (3'-LM1) and a further ganglioside of the neolacto-series with an extra lactosamine moiety. The only oligosialoganglioside detected was IV3, II3-alpha-NeuAc2-GgOse4Cer (GD1a).
authors.affiliation: Department of Psychiatry and Neurochemistry, Gothenburg University, St Jorgens Hospital, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Gottfries
authors.forename: J E
authors.initials: JE
authors.lastname: Mansson
authors.forename: P
authors.initials: P
authors.lastname: Fredman
authors.forename: C J
authors.initials: CJ
authors.lastname: Wikstrand
authors.forename: H S
authors.initials: HS
authors.lastname: Friedman
authors.forename: D D
authors.initials: DD
authors.lastname: Bigner
authors.forename: L
authors.initials: L
authors.lastname: Svennerholm
mesh_terms: D000818:Animals; D002528:Cerebellar Neoplasms / Q000378:metabolism*; D002849:Chromatography, Gas; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D008527:Medulloblastoma / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014018:Tissue Distribution; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D005732:Gangliosides