title: Automated enhanced latex agglutination assay for rheumatoid factors in serum.
abstract: This improved assay of rheumatoid factors in serum, described here for use with the Baker "Encore" centrifugal analyzer, is efficient, with 250-sample throughput per hour; reproducible, with between-batch CV = 5% and within-batch CV = 2% (mid-assay range); and results correlate well (r = 0.9) with those by other methods. The method is fully quantitative and automated, involves no predilution steps, and can be adapted for use in a wide range of systems. It has a sensitivity of 96% and specificity of 80% in diagnosing rheumatoid arthritis.
authors.affiliation: Department of Biochemistry, Selly Oak Hospital, Birmingham, U.K.
authors.forename: J W
authors.initials: JW
authors.lastname: Winkles
authors.forename: J
authors.initials: J
authors.lastname: Lunec
authors.forename: L
authors.initials: L
authors.lastname: Gray
mesh_terms: D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000276:immunology*; D001322:Autoanalysis; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods; D012015:Reference Standards; D012217:Rheumatoid Factor / Q000032:analysis*
chemical_list: D012217:Rheumatoid Factor