title: Identification of 5-HT3 recognition sites in the ferret area postrema.
abstract: The 5-HT3 receptor antagonist [3H]zacopride was used to identify 5-HT3 recognition sites in the ferret area postrema. Specific binding was determined by the inclusion of the 5-HT3 receptor antagonist BRL 43694 in the incubation media, and was shown to be much higher in the area postrema than in other brain regions. The increased binding in the area postrema may reflect either a greater number of binding sites, a higher affinity for such sites, or both. The results indicate that 5-HT3 recognition sites are present within the area postrema and may afford an antiemetic site of action for zacopride and other 5-HT3 receptor antagonists.
authors.affiliation: Postgraduate School of Studies in Pharmacology, University of Bradford, UK.
authors.forename: N M
authors.initials: NM
authors.lastname: Barnes
authors.forename: B
authors.initials: B
authors.lastname: Costall
authors.forename: R J
authors.initials: RJ
authors.lastname: Naylor
authors.forename: F D
authors.initials: FD
authors.lastname: Tattersall
mesh_terms: D000818:Animals; D001549:Benzamides; D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002335:Carnivora / Q000378:metabolism*; D002552:Cerebral Ventricles / Q000378:metabolism*; D005260:Female; D005289:Ferrets / Q000378:metabolism*; D017829:Granisetron; D066298:In Vitro Techniques; D007191:Indazoles; D011869:Radioligand Assay; D011985:Receptors, Serotonin / Q000145:classification / Q000378:metabolism*; D012702:Serotonin Antagonists
chemical_list: D001549:Benzamides; D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D017829:Granisetron; D007191:Indazoles; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; C055971:zacopride