title: Recovery of motile sperm using the migration-sedimentation technique in an in-vitro fertilization-embryo transfer programme.
abstract: Sperm washing techniques, based on the swim-up principle used before inseminating the human oocyte in in-vitro fertilization and embryo transfer programmes (IVF-ET), usually require prior centrifugation which causes damage to the sperm cell. A technique is described for separating sperm at laboratory temperature based on sperm migration--sedimentation principles, using two concentric tubes and recovering 70-90% forward-moving cells. A group of 17 patients is presented who were managed with this method. The results were 85% fertilization rate, 4% polyspermia and six clinical pregnancies.
authors.affiliation: Centro Colombiano de Fértilidad y Esterilidad, Bogotá.
authors.forename: E
authors.initials: E
authors.lastname: Lucena
authors.forename: C
authors.initials: C
authors.lastname: Lucena
authors.forename: M
authors.initials: M
authors.lastname: Gómez
authors.forename: J A
authors.initials: JA
authors.lastname: Ortiz
authors.forename: J
authors.initials: J
authors.lastname: Ruiz
authors.forename: A
authors.initials: A
authors.lastname: Arango
authors.forename: C
authors.initials: C
authors.lastname: Diaz
authors.forename: C
authors.initials: C
authors.lastname: Beuerman
mesh_terms: D002469:Cell Separation; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*