Ultraviolet irradiation of platelet concentrate abrogates lymphocyte activation without affecting platelet function in vitro.
We studied the effect of ultraviolet (UV) radiation on platelet concentrates. Samples irradiated at 310 mm for 30 minutes at a dose of 1782 J per m2 showed no loss of platelet function in vitro as determined by adenosine diphosphate, collagen, or ristocetin-induced aggregation. Lymphocytes isolated from irradiated units were unable to act as responders or stimulators in a mixed-lymphocyte reaction. These data suggest that UV radiation of platelet concentrates may result in a cell suspension that is unable to evoke an immunological response.
Kahn
R A
RA
Duffy
B F
BF
Rodey
G G
GG
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology / Q000528:radiation effects*; D001803:Blood Transfusion; D003094:Collagen / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D010974:Platelet Aggregation / Q000187:drug effects; D017713:Platelet Transfusion; D012310:Ristocetin / Q000494:pharmacology; D014466:Ultraviolet Rays
D012310:Ristocetin; D000244:Adenosine Diphosphate; D003094:Collagen