Interactions of factors bound at two different sites in the 5'-upstream region of the adenovirus 12 E1A gene.
A nuclear extract of Ehrlich ascites tumor cell contains factors binding to two distinct sites in the 5'-upstream region of the adenovirus 12 E1A gene. The gel shift assay was performed for characterization of the binding factors with oligo DNA probes containing sequences corresponding to these sites. The specific binding of a factor to one probe was enhanced when the other oligo DNA was present in excess in the binding reaction. Thus possibly, protein-protein interactions between factors may mutually prevent their binding to target sequences.
Nakanishi
Y
Y
Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa, Japan.
Koikeda
S
S
Shibata
H
H
Masamune
Y
Y
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis; D015342:DNA Probes; D004279:DNA, Viral / Q000378:metabolism*; D005814:Genes, Viral; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D015342:DNA Probes; D004279:DNA, Viral; D014157:Transcription Factors