The human transforming growth factor alpha promoter directs transcription initiation from a single site in the absence of a TATA sequence.
Transforming growth factor alpha (TGF-alpha) is a transformation-responsive mitogenic polypeptide that is expressed in the brain, epithelial cells, and activated macrophages. We isolated and characterized the TGF-alpha promoter and localized the 5' end of the TGF-alpha transcript to a unique position. Surprisingly, no apparent TATA box was present in the promoter sequence, suggesting that transcription from mammalian genes can initiate at unique and specific positions from promoters lacking this sequence motif.
Jakobovits
E B
EB
Department of Cell Genetics, Genentech, Inc., South San Francisco, California 94080.
Schlokat
U
U
Vannice
J L
JL
Derynck
R
R
Levinson
A D
AD
D000595:Amino Acid Sequence; D001483:Base Sequence; D002678:Chimera; D005796:Genes; D005801:Genes, Homeobox; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D014158:Transcription, Genetic; D015291:Transforming Growth Factors / Q000235:genetics*
D012333:RNA, Messenger; D015291:Transforming Growth Factors