A first screening for hemocompatibility of a universal support for selective and specific hemoperfusion.
A modified filmadsorber is presented, intended to be used for selective and specific hemoperfusion. It consists of a spirally wound cellulose nitrate film, onto which--after chemical activation with sodium periodate--albumin is bound, stabilized, sterilized and activated with glutaraldehyde. Various bioactive ligands containing amino groups can be coupled to this support. A subsequent treatment with dimethylamino borane stabilizes the bonds between cellulose nitrate, albumin, glutaraldehyde and ligand. Columns in which a second layer of albumin is bound to the support as a model for a bioactive ligand were first screened for hemocompatibility using rabbits. Leukocyte, thrombocyte and hematocrit behaviour during hemoperfusion showed that hemocompatibility of the support was good.
Verhoeven
M L
ML
Department of Analytical Chemistry, Faculty of Chemical Technology, Eindhoven University of Technology, the Netherlands.
Böttcher
M
M
Everaerts
F M
FM
Sluyterman
L A
LA
Ernst
B
B
Klinkmann
H
H
D000327:Adsorption; D000418:Albumins; D000596:Amino Acids; D000818:Animals; D002482:Cellulose; D006464:Hemoperfusion / Q000295:instrumentation*; D007958:Leukocyte Count; D008024:Ligands; D010976:Platelet Count; D011817:Rabbits
D000418:Albumins; D000596:Amino Acids; D008024:Ligands; D002482:Cellulose