Evidence for the involvement of dithiol groups in mitochondrial calcium transport: studies with cadmium.
The effect of cadmium on some functions of mitochondria isolated from kidneys of rat was studied. Addition of cadmium chloride to mitochondria induced stimulation of both State 4 respiratory rate and ATPase activity, which are prevented by the addition of ruthenium red. We also show that cadmium inhibits competitively calcium translocation; this inhibitory effect of cadmium is reverted by the addition of dithiothreitol. From these results, it is proposed that, similarly to Ca2+, cadmium penetrates mitochondria and binds to a membrane dithiol group, which is essential for the translocation of the cation.
Chávez
E
E
Briones
R
R
Michel
B
B
Bravo
C
C
Jay
D
D
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002104:Cadmium / Q000494:pharmacology*; D019256:Cadmium Chloride; D002122:Calcium Chloride / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology*; D004285:Dogs; D007425:Intracellular Membranes / Q000502:physiology; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013367:Submitochondrial Particles / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
D013438:Sulfhydryl Compounds; D002104:Cadmium; D000252:Calcium-Transporting ATPases; D019256:Cadmium Chloride; D002122:Calcium Chloride; D004229:Dithiothreitol