[Identification of digitonin binding sites on the plasma membrane of the rabbit aorta endotheliocytes].
Specific reagents comprising digitonin bound to latex spheres were used as visual markers for the detection of cholesterol sites on the endothelial cell surface by scanning electron microscopy. The distribution of latex markers in the plasmolemma of the endothelial cells was investigated. These markers have strong bonds with the ligands. This interaction guarantees high specificity of binding between labeled markers and cellular membrane cholesterol.
Popova
E G
EG
Povaliĭ
T M
TM
Gusev
S A
SA
Gritskova
I A
IA
Kogan
E M
EM
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D004072:Digitonin / Q000378:metabolism*; D004727:Endothelium / Q000378:metabolism; D007202:Indicators and Reagents; D007840:Latex; D008855:Microscopy, Electron, Scanning; D011817:Rabbits
D007202:Indicators and Reagents; D007840:Latex; D002784:Cholesterol; D004072:Digitonin
Identifikatsiia digitoninsviazyvaiushchikh uchastkov plasmaticheskoĭ membrany éndoteliotsitov aorty krolika.