Administration of D-aspartate increases D-aspartate oxidase activity in mouse liver.
Oral administration of D-aspartate to mice for 2 weeks by addition of the amino acid to drinking water produced a nearly 4-fold increase in liver D-aspartate oxidase (EC 1.4.3.1) activity, whereas no increase was induced by L-aspartate administered in the same way. Administration of D-aspartate also produced a small significant increase in the kidney enzyme activity, but L-aspartate administration increased the activity as well. The enzyme activity in the brain and muscle was not affected by administration of either D- or L-aspartate. Intraperitoneal administration of D-aspartate increased the enzyme activity only in the liver, and other compounds tested, including D-glutamate and D-alanine, could not replace D-aspartate. The results indicate a specific relationship between D-aspartate and D-aspartate oxidase and suggest that the amino acid is, in fact, a physiological substrate of the enzyme.
Yamada
R
R
Department of Biochemistry, Kyoto Prefectural University of Medicine, Japan.
Nagasaki
H
H
Nagata
Y
Y
Wakabayashi
Y
Y
Iwashima
A
A
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000008:administration & dosage*; D001921:Brain / Q000201:enzymology; D050804:D-Aspartate Oxidase; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009132:Muscles / Q000201:enzymology
D001224:Aspartic Acid; D000594:Amino Acid Oxidoreductases; D050804:D-Aspartate Oxidase