title: Immunocytochemistry of four mixed pituitary adenomas and intrasellar gangliocytomas associated with different clinical syndromes: acromegaly, amenorrhea-galactorrhea, Cushing's disease and isolated tumoral syndrome.
abstract: Four tumors consisting of pituitary adenomatous cells (AD) intricated with ganglion cells (GC) were studied. Each case was associated with a different clinical syndrome: acromegaly, amenorrhea-galactorrhea, Cushing's disease and isolated tumoral syndrome with no hormonal hypersecretion. (a) In the case with acromegaly, immunoreactive growth hormone (IR-GH) was present in 80% of AD. IR-vasoactive intestinal peptide (VIP) was found in 5%-10% of AD and in few GC. Rare GC and processes showed IR-GH-releasing hormone (GRH), -somatostatin (SRIH), -gonadotropin-releasing hormone and -adrenocorticotropin-releasing hormone. (b) In the case with amenorrhea-galactorrhea, IR-prolactin (PRL) was seen in 90% of AD. IR-PRL and -VIP were present in rare GC. (c) In the case with Cushing's disease, 60% of AD and very few GC contained IR-adrenocorticotropin (ACTH) and beta-lipotropin. Rare GC processes contained IR-SRIH. (d) In the case without pituitary hormone hypersecretion, PRL was localized in rare AD and GC. Pituitary hormone and neuropeptides were never colocalized in the same cells. No case displayed IR-neurophysins or -thyroliberin. Pituitary hormones were localized by ultrastructural immunogold labeling. These findings show that: (i) in three cases, pituitary hormones (PRL and ACTH), and, in one case, VIP could be localized in both adenomatous and ganglion cells; (ii) the pituitary hormone-containing cells in the tumors could be related to the hypersecretory syndromes; (iii) intratumoral IR-VIP and -GRH might be involved in GH and PRL hypersecretion in the cases with acromegaly and amenorrhea-galactorrhea.
authors.affiliation: INSERM U.223 Faculté de Médecine Pitié-Salpêtrière, Paris, France.
authors.forename: J Y
authors.initials: JY
authors.lastname: Li
authors.forename: O
authors.initials: O
authors.lastname: Racadot
authors.forename: M
authors.initials: M
authors.lastname: Kujas
authors.forename: M
authors.initials: M
authors.lastname: Kouadri
authors.forename: F
authors.initials: F
authors.lastname: Peillon
authors.forename: J
authors.initials: J
authors.lastname: Racadot
mesh_terms: D000172:Acromegaly / Q000150:complications; D000236:Adenoma / Q000150:complications / Q000000981:diagnostic imaging / Q000378:metabolism*; D000328:Adult; D000568:Amenorrhea / Q000150:complications; D003480:Cushing Syndrome / Q000150:complications; D005260:Female; D005687:Galactorrhea / Q000150:complications; D005729:Ganglioneuroma / Q000150:complications / Q000000981:diagnostic imaging / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010911:Pituitary Neoplasms / Q000150:complications / Q000000981:diagnostic imaging / Q000378:metabolism*; D011247:Pregnancy; D011859:Radiography; D012658:Sella Turcica / Q000000981:diagnostic imaging*; D015043:Zollinger-Ellison Syndrome / Q000150:complications