beta-Carotene reduces sister chromatid exchanges induced by chemical carcinogens in mouse mammary cells in organ culture.
Present studies in the mammary epithelial cell transformation model in organ culture showed that presence of beta-carotene during the 24 hr treatment (initiation stage) of the glands with the carcinogens, 7,12-dimethylbenz[a]anthracene (DMBA), N-nitrosodiethylamine (DENA) and N-methylnitrosourea (MNU), caused a highly significant (P less than 0.001-0.01) reduction of SCE induced by the same carcinogens. In contrast, 4-hydroxyphenyl retinamide (4-HPR) which is known to act at the promotional stage of carcinogenesis did not show any significant reduction of SCE. Thus findings suggest that beta-carotene can modify the DNA damaging effect of the carcinogens and thereby may also prevent the initiation of the carcinogenic process.
Manoharan
K
K
Banerjee
M R
MR
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000037:antagonists & inhibitors; D000818:Animals; D002273:Carcinogens / Q000037:antagonists & inhibitors*; D002338:Carotenoids / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004052:Diethylnitrosamine / Q000037:antagonists & inhibitors; D005260:Female; D017313:Fenretinide; D008321:Mammary Glands, Animal / Q000166:cytology*; D008770:Methylnitrosourea / Q000037:antagonists & inhibitors; D051379:Mice; D009924:Organ Culture Techniques; D012176:Retinoids / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D014212:Tretinoin / Q000031:analogs & derivatives / Q000494:pharmacology; D019207:beta Carotene
D002273:Carcinogens; D012176:Retinoids; D019207:beta Carotene; D017313:Fenretinide; D002338:Carotenoids; D004052:Diethylnitrosamine; D014212:Tretinoin; D015127:9,10-Dimethyl-1,2-benzanthracene; D008770:Methylnitrosourea