title: Immunoglobulin G is a platelet alpha granule-secreted protein.
abstract: It has been known for 27 yr that blood platelets contain IgG, yet its subcellular location and significance have never been clearly determined. In these studies, the location of IgG within human platelets was investigated by immunocytochemical techniques and by the response of platelet IgG to agents that cause platelet secretion. Using frozen thin-sections of platelets and an immunogold probe, IgG was located within the alpha-granules. Thrombin stimulation caused parallel secretion of platelet IgG and two known alpha-granule proteins, platelet factor 4 and beta-thromboglobulin, beginning at 0.02 U/ml and reaching 100% at 0.5 U/ml. Thrombin-induced secretion of all three proteins was inhibited by prostaglandin E1 and dibutyryl-cyclic AMP. Calcium ionophore A23187 also caused parallel secretion of all three proteins, whereas ADP caused virtually no secretion of any of the three. From these data and a review of the literature, we hypothesize that plasma IgG is taken up by megakaryocytes and delivered to the alpha-granules, where it is stored for later secretion by mature platelets.
authors.forename: J N
authors.initials: JN
authors.lastname: George
authors.forename: S
authors.initials: S
authors.lastname: Saucerman
authors.forename: S P
authors.initials: SP
authors.lastname: Levine
authors.forename: L K
authors.initials: LK
authors.lastname: Knieriem
authors.forename: D F
authors.initials: DF
authors.lastname: Bainton
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000418:Albumins / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D000001:Calcimycin / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000378:metabolism; D006046:Gold; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000378:metabolism; D007158:Immunologic Techniques; D010978:Platelet Factor 4 / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D001620:beta-Thromboglobulin / Q000378:metabolism
chemical_list: D000418:Albumins; D007074:Immunoglobulin G; D001620:beta-Thromboglobulin; D010978:Platelet Factor 4; D000001:Calcimycin; D000244:Adenosine Diphosphate; D006046:Gold; D013917:Thrombin