Transcriptional unit of the murine Thy-1 gene: different distribution of transcription initiation sites in brain.
Structural analysis of the mouse Thy-1.2 gene has shown that the major promoter of the gene is characterized by a tissue-specific DNase I-hypersensitive site and is located within a methylation-free island. The gene is regulated at the transcriptional level, and steady-state mRNA analysis reveals that the previously reported exon Ib contributes at most 5% of the total mRNA. The major promoter uses several transcription initiation sites within a region of 100 base pairs. The frequency of usage of these sites in brain is markedly different from that in other tissues.
Spanopoulou
E
E
Laboratory of Gene Structure and Expression, National Institute for Medical Research, Mill Hill, London, United Kingdom.
Giguere
V
V
Grosveld
F
F
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D002843:Chromatin / Q000648:ultrastructure; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; D005091:Exons; D005796:Genes; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D018800:Thy-1 Antigens; D014158:Transcription, Genetic
D000954:Antigens, Surface; D002843:Chromatin; D018800:Thy-1 Antigens; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I