17 beta-estradiol secretion in normal and hypophysectomized chick embryos.
Ovaries from decapitated, sham-operated and intact 18-day-old chick embryos were cultured in Medium 199 for 6 h, and the amount of 17 beta-estradiol released into the culture media was determined by radioimmunoassay. Ovaries from decapitated embryos secreted significantly lower amounts of 17 beta-estradiol than those from intact embryos, but there was no difference when they were compared to ovaries from sham-operated embryos in this respect. On an ovarian weight basis, 17 beta-oestradiol production was significantly different between the 3 groups of embryos, the ratio being highest in the decapitates. 17 beta-Estradiol concentration was the same in serum from both decapitated and intact female embryos. Considering these results, it is concluded that the hypophysis does not control 17 beta-estradiol secretion by the chick embryo ovary even near hatching time.
Weniger
J P
JP
Laboratoire de Zoologie et d'Embryologie Expérimentale de l'Université Louis-Pasteur, Strasbourg, France.
Chouraqui
J
J
Zeis
A
A
D000818:Animals; D002642:Chick Embryo / Q000502:physiology*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007016:Hypophysectomy; D009924:Organ Culture Techniques; D010053:Ovary / Q000378:metabolism
D004958:Estradiol