title: Hydroxyl negative chemical ionization mass spectrometry linked with collisionally activated decomposition. A modern analytical tool in inborn errors of metabolism.
abstract: Two kinds of inborn errors of metabolism, dicarboxylic aciduria and hyperoxaluria, have been studied by means of hydroxyl negative ion chemical ionization [NICI(OH-)], linked with collisionally activated decomposition experiments on the [M-H]- species of the pathognomonic organic acids. This method has led to non-controversial qualitative determinations of C4-C10 dicarboxylic acids and oxalic, glyceric and glyoxylic acids. NICI(OH-) linked with collisionally activated decomposition mass analysed ion kinetic energy spectrometry (CAD MIKES) is proposed herein for diagnostic purposes, as a valid mass spectrometric alternative to standard gas chromatographic/mass spectrometric analysis. The procedure is characterized by simplified sample treatment and by fast execution.
authors.forename: P
authors.initials: P
authors.lastname: Rinaldo
authors.forename: G
authors.initials: G
authors.lastname: Miolo
authors.forename: L
authors.initials: L
authors.lastname: Chiandetti
authors.forename: F
authors.initials: F
authors.lastname: Zacchello
authors.forename: S
authors.initials: S
authors.lastname: Daolio
authors.forename: P
authors.initials: P
authors.lastname: Traldi
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013058:Mass Spectrometry; D008661:Metabolism, Inborn Errors / Q000378:metabolism*