Prenatal diagnosis of thalassemia: experiences at the Shanghai Children's Hospital.
Using DNA dot-blot hybridization, restriction endonuclease gene mapping with oligonucleotide probes, restriction fragment length polymorphism linkage analysis, and hybridization, prenatal diagnosis was performed for 32 pregnancies at risk for alpha-thalassemia and for 10 pregnancies at risk for beta-thalassemia. The DNA samples were prepared from chorion villi or amniotic fluid cells.
Zeng
Y T
YT
Laboratory of Medical Genetics, Shanghai Children's Hospital, P.R. China.
Huang
S Z
SZ
Zhang
M L
ML
D000650:Amnion / Q000166:cytology; D002681:China; D015193:Chorionic Villi Sampling; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011296:Prenatal Diagnosis; D015183:Restriction Mapping; D013789:Thalassemia / Q000175:diagnosis*
D009841:Oligonucleotides; D004247:DNA