Modified photometric method for the determination of phospholipase A activities.
A modified photometric method for the determination of phospholipase A activities which is based on a previously published reaction principle is described. The modified assay uses a lyophilized substrate emulsion rather than a freshly prepared phospholipid emulsion and a new chromogen (tribromohydroxybenzoic acid and 4-amino-antipyrine) with a high molar absorption coefficient (1,930 m2/mol at 546 nm, 2,900 m2/mol at 512 nm wavelength). The new test is more practicable with respect to pipetting volumes and incubation times. Preliminary results of a method evaluation indicate that the modified assay fulfills the usual criteria for clinical chemical enzyme measurements.
Hoffmann
G E
GE
Institute of Clinical Chemistry, Bogenhausen Hospital, München.
Neumann
U
U
D006801:Humans; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D010783:Photometry / Q000295:instrumentation / Q000379:methods*
D010740:Phospholipases; D010741:Phospholipases A