title: Image reconstruction of the flagellar basal body of Caulobacter crescentus.
abstract: The bacterium Caulobacter crescentus has a single polar flagellum, which is present for only a portion of its cell cycle. The flagellum is ejected from the swarmer cell and then synthesized de novo later in the cell cycle. The flagellum is composed of a transmembrane basal body, a hook and a filament. Single-particle averaging and image reconstruction methods were applied to the electron micrographs of negatively stained basal bodies from C. crescentus. These basal bodies have five rings threaded on a rod. The L and P rings are connected by a bridge of material at their outer radii. The E ring is a thin, flat disk. The S ring has a triangular cross section, the sides of the triangle abutting the E ring, the rod and the M ring. The M ring, which is at the inner membrane of the cell, has a different structure depending on the method of preparation. With one method, the M ring makes a snug contact with the S ring and is often capped by an axial button, a new component apparently distinct from the M ring. With the other method, the M ring is similar to that of S. typhimurium; that is, it contacts the S ring only at an outer radius and lacks the button. Averages of the rod-hook-filament subassembly ejected by swarmer cells reveal that the rod consists of two parts with the E ring marking the approximate position of the break. The structures of basal bodies from two mutants defective in the hook assembly were found to be indistinguishable from wild-type basal bodies, suggesting that the assembly of the basal body is independent of the hook or filament assembly.
authors.affiliation: Department of Biology, Rosenstiel Basic Medical Sciences, Brandeis University, Waltham, MA 02254.
authors.forename: M J
authors.initials: MJ
authors.lastname: Stallmeyer
authors.forename: K M
authors.initials: KM
authors.lastname: Hahnenberger
authors.forename: G E
authors.initials: GE
authors.lastname: Sosinsky
authors.forename: L
authors.initials: L
authors.lastname: Shapiro
authors.forename: D J
authors.initials: DJ
authors.lastname: DeRosier
mesh_terms: D001419:Bacteria / Q000648:ultrastructure*; D005407:Flagella / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008854:Microscopy, Electron; D008961:Models, Structural; D009154:Mutation