Effects of cis-dichloro-diammine-platinum (II) (cis-DDP) on Epstein-Barr virus induction and cell differentiation.
Morphological changes were induced by cis-dichlorodiammine platinum (II) (cis-DDP) in two Epstein-Barr virus (EBV) transformed cell lines: the productive P3HR-1 and the nonproductive Raji cell line. In P3HR-1 cells cis-DDP induced synthesis of viral antigens, viral particles and morphological changes characteristic for virus replication. In Raji cells, the virus replicative cycle was not induced and virus-specific morphological changes were limited to the sporadic appereance of some very early alterations in cell morphology. However, in Raji cells, but not in P3HR-1 cells, up to 20 per cent of the cell population exhibited differentiation-related changes towards plasma cell morphology. The most advances stage of cell differentiation detected was classified as plasmablast. Cis-DDP also induced some changes associated with the cytostatic effect of the drug. In the treated cell population cytokinesis was inhibited and frequently multinuclear cells appeared; moreover, extensive degenerative changes were observed.
Anisimová
E
E
Department of Experimental Virology, Institute of Sera and Vaccines, Prague, Czechoslovakia.
Nováková
Z
Z
Roubal
J
J
D000956:Antigens, Viral / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D002472:Cell Transformation, Viral / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects; D004854:Herpesvirus 4, Human / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D008938:Mitosis / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000956:Antigens, Viral; D002945:Cisplatin