title: Ultrastructural localisation of creatine kinase activity in the contact sites between inner and outer mitochondrial membranes of rat myocardium.
abstract: The mitochondrial isoenzyme of creatine kinase, together with the ADP/ATP translocase, most probably belongs to a functional multi-enzyme complex located on the inner mitochondrial membrane. The outer membrane is a necessary constituent of this microcompartment. On the other hand, electron microscopic visualisation demonstrated the formation of contact sites between inner and outer mitochondrial membranes as a reaction to variations of the energy metabolism. In search for a possible correlation between these biochemical and morphological phenomena, rat myocardia were brought into the required energy state by stimulation through catecholaminergic mechanisms or adjusted perfusion with amytal. Subsequently, creatine kinase was cytochemically localised. Creatine kinase activity is demonstrated in membrane contacts between inner and outer mitochondrial membranes. The extent of contact sites and creatine kinase activity depends on the metabolic state as shown by morphometric analysis of the surface density of cytochemical reaction product. This surface density diminishes drastically after inhibiting the metabolic activity with amytal. It is concluded that these contact sites are dynamic micro-environments in which the active site of creatine kinase, oxidative phosphorylation and ADP/ATP transport interact during basal and stimulated metabolism.
authors.affiliation: Department of Medicine, University of Antwerp, Wilrijk, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: Biermans
authors.forename: I
authors.initials: I
authors.lastname: Bernaert
authors.forename: M
authors.initials: M
authors.lastname: De Bie
authors.forename: B
authors.initials: B
authors.lastname: Nijs
authors.forename: W
authors.initials: W
authors.lastname: Jacob
mesh_terms: D000654:Amobarbital / Q000494:pharmacology; D000818:Animals; D003402:Creatine Kinase / Q000032:analysis*; D004579:Electron Transport / Q000187:drug effects; D004734:Energy Metabolism; D005260:Female; D006651:Histocytochemistry; D007425:Intracellular Membranes / Q000201:enzymology* / Q000648:ultrastructure; D007527:Isoenzymes; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007527:Isoenzymes; D003402:Creatine Kinase; D000654:Amobarbital