title: A scanning electron microscopic study of the common bile duct epithelium in the rabbit during experimental biliary tract obstruction.
abstract: Complete biliary obstruction was induced in rabbits by distal ligation of the common bile duct (CBD). The epithelial surface of the CBD was studied with scanning electron microscopy (SEM) at 1-6 days post-ligation. Concomitant biochemical assessments and pressure measurements in the CBD were performed. SEM of non-ligated CBD showed that the epithelium is arranged in folds and clefts, the latter displaying interdigitating cellular processes. At 2 days post-ligation, the epithelial clefts were focally distended showing marked dehiscence of the cellular processes with exposure of the subepithelial connective tissue layer. At 4-6 days post-ligation, extensive defects in the epithelium were observed, frequently with a sharp demarcation between intact epithelium and exposed connective tissue stroma.
authors.affiliation: Department of Surgery, University of Amsterdam, The Netherlands.
authors.forename: T M
authors.initials: TM
authors.lastname: van Gulik
authors.forename: N J
authors.initials: NJ
authors.lastname: Lygidakis
authors.forename: J
authors.initials: J
authors.lastname: van Marle
authors.forename: J
authors.initials: J
authors.lastname: James
authors.forename: P J
authors.initials: PJ
authors.lastname: Klopper
authors.forename: M N
authors.initials: MN
authors.lastname: van der Heyde
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D002779:Cholestasis / Q000473:pathology*; D001651:Cholestasis, Extrahepatic / Q000473:pathology; D003135:Common Bile Duct / Q000648:ultrastructure*; D004848:Epithelium / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D001663:Bilirubin