title: Reperfusion injury in the lung preserved for 24 hours.
abstract: The left lower lobes of 28 canine lungs were isolated, preserved, and then reperfused for 150 minutes. Five groups of lobes were studied: group 1, control (n = 5); group 2, one hour of warm ischemia (n = 5); group 3, one hour of warm ischemia + oxygen free radical scavengers (n = 5); group 4, 24 hours of cold ischemia (n = 8); and group 5, 24 hours of cold ischemia + oxygen free radical scavengers (n = 5). Oxygen free radical scavengers consisted of superoxide dismutase and catalase (100 micrograms/mL) given at the moment of reflow. Extravascular lung water (grams per gram of blood-free dry lobe weight) after reperfusion was 2.75 +/- 0.19, 5.46 +/- 0.60, 4.08 +/- 0.37, 9.43 +/- 0.98, and 6.91 +/- 0.95 for groups 1 through 5, respectively (p less than 0.05, groups 2 through 5 versus group 1; p less than 0.05, group 2 versus group 3 and group 4 versus group 5). Lung tissue lipid peroxidation, measured as thiobarbituric acid reactive material, was 117 +/- 14, 314 +/- 19, and 163 +/- 25 nmol/g dry lobe weight for groups 1, 4, and 5, respectively (p less than 0.05, group 4 versus group 1 and group 4 versus group 5). The data suggest that oxygen free radical scavengers attenuate reperfusion injury after long-term hypothermic lung preservation.
authors.affiliation: Division of Cardiothoracic Surgery, University of North Carolina School of Medicine, Chapel Hill 27599-7065.
authors.forename: D E
authors.initials: DE
authors.lastname: Paull
authors.forename: B A
authors.initials: BA
authors.lastname: Keagy
authors.forename: E J
authors.initials: EJ
authors.lastname: Kron
authors.forename: B R
authors.initials: BR
authors.lastname: Wilcox
mesh_terms: D000818:Animals; D002374:Catalase / Q000494:pharmacology; D004285:Dogs; D005609:Free Radicals; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D009926:Organ Preservation / Q000379:methods; D009929:Organ Size / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D011652:Pulmonary Circulation / Q000187:drug effects; D011654:Pulmonary Edema / Q000209:etiology / Q000473:pathology; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013997:Time Factors
chemical_list: D005609:Free Radicals; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen