title: Induction and characterization of mitochondrial DNA mutants in Chlamydomonas reinhardtii.
abstract: In addition to lethal minute colony mutations which correspond to loss of mitochondrial DNA, acriflavin induces in Chlamydomonas reinhardtii a low percentage of cells that grow in the light but do not divide under heterotrophic conditions. Two such obligate photoautotrophic mutants were shown to lack the cyanide-sensitive cytochrome pathway of the respiration and to have a reduced cytochrome c oxidase activity. In crosses to wild type, the mutations are transmitted almost exclusively from the mating type minus parent. A same pattern of inheritance is seen for the mitochondrial DNA in crosses between the two interfertile species C. reinhardtii and Chlamydomonas smithii. Both mutants have a deletion in the region of the mitochondrial DNA containing the apocytochrome b gene and possibly the unidentified URFx gene.
authors.affiliation: Département de Botanique, Université de Liège, Sart Tilman, Belgium.
authors.forename: R F
authors.initials: RF
authors.lastname: Matagne
authors.forename: M R
authors.initials: MR
authors.lastname: Michel-Wolwertz
authors.forename: C
authors.initials: C
authors.lastname: Munaut
authors.forename: C
authors.initials: C
authors.lastname: Duyckaerts
authors.forename: F
authors.initials: F
authors.lastname: Sluse
mesh_terms: D000167:Acriflavine / Q000494:pharmacology; D002696:Chlamydomonas / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D003433:Crosses, Genetic; D004272:DNA, Mitochondrial / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D010101:Oxygen Consumption; D015183:Restriction Mapping
chemical_list: D004272:DNA, Mitochondrial; D000167:Acriflavine