Isolation and reconstitution of the high-affinity choline carrier.
Monoclonal antibodies, which block the high-affinity uptake of choline in synaptosomal ghosts, have been used to purify a membrane polypeptide (80 kDa) from insect synaptosomal membranes. This isolated protein was found to catalyse the sodium-dependent, hemicholinium-sensitive accumulation of choline after reconstitution into liposomes, thus, apparently represents the high-affinity choline transporter.
Knipper
M
M
University Stutgart-Hohenheim, Institute for Zoophysiology, FRG.
Boekhoff
I
I
Breer
H
H
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002794:Choline / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006110:Grasshoppers / Q000032:analysis*; D006426:Hemicholinium 3 / Q000494:pharmacology; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D012964:Sodium / Q000494:pharmacology; D013570:Synaptic Membranes / Q000032:analysis; D013574:Synaptosomes / Q000032:analysis*
D002352:Carrier Proteins; D008081:Liposomes; D008565:Membrane Proteins; D006426:Hemicholinium 3; D012964:Sodium; D002794:Choline