The effect of homogenization temperature upon the apparent cellular compartmentalization of unoccupied estrogen receptor.
Homogenization of rat uterus at elevated temperatures results in an increased nuclear localization of unoccupied estrogen receptor. This is a nonlinear effect which is accounted for by an increased population of KCl-resistant nuclear binding sites at the elevated homogenization temperatures.
Campbell
P S
PS
Department of Biological Sciences, University of Alabama, Huntsville 35899.
Swanson
K A
KA
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D011189:Potassium Chloride; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000378:metabolism*; D013696:Temperature; D014599:Uterus / Q000648:ultrastructure*
D011960:Receptors, Estrogen; D011189:Potassium Chloride