Intraseptal microinjection of adrenocorticotropic hormone1-24 antagonizes pentobarbital-induced narcosis and depression of hippocampal cholinergic activity.
Intracerebroventricular or intraseptal microinjection of adrenocorticotropic hormone1-24 (ACTH1-24) to pentobarbital-anesthetized rats and rabbits produced shortening of the duration of narcosis. This analeptic effect was blocked by atropine, indicating the central cholinergic nature of the response. ACTH1-24 also increased hippocampal sodium-dependent high affinity choline uptake activity that had been depressed by the barbiturate. Naloxone-pretreatment also blocked the analeptic and cholinergic activating properties of ACTH1-24. These results suggest that intraseptal ACTH1-24 produces its analeptic effect by activating a hippocampal cholinergic arousal system.
Horita
A
A
Department of Pharmacology, University of Washington School of Medicine, Seattle.
Carino
M A
MA
D000324:Adrenocorticotropic Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D000758:Anesthesia; D000818:Animals; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D002794:Choline / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects*; D008297:Male; D008845:Microinjections; D009270:Naloxone / Q000494:pharmacology; D010275:Parasympathetic Nervous System / Q000187:drug effects*; D010424:Pentobarbital / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012688:Septum Pellucidum / Q000187:drug effects*
D000697:Central Nervous System Stimulants; D010446:Peptide Fragments; D009270:Naloxone; D000324:Adrenocorticotropic Hormone; D010424:Pentobarbital; D002794:Choline