Lack of D-amino-acid oxidase activity causes a specific renal aminoaciduria in the mouse.
Thin-layer chromatography and amino acid analysis showed that urine of mutant ddY/DAO- mice lacking D-amino-acid oxidase activity contained more serine, proline, alanine and methionine than that of normal ddY/DAO+ mice. Among these four, an increase in alanine was conspicuous. However, the urinary levels of 11 other amino acids and glucose were not different between the ddY/DAO- and ddY/DAO+ mice. Amino acid analysis showed that the plasma levels of serine, proline and methionine were not elevated in the ddY/DAO- mice, though a slight increase in alanine was observed. Genetic crosses showed that aminoaciduria and lack of D-amino-acid oxidase activity were concomitantly transmitted as a set through generations. These results indicated that the lack of enzyme activity caused a specific renal aminoaciduria. Whether this enzyme merely diminishes the D-amino acid load presented for reabsorption, or actually participates catalytically in the reabsorption process, remains undetermined.
Konno
R
R
Department of Microbiology, Dokkyo University School of Medicine, Tochigi, Japan.
Isobe
K
K
Niwa
A
A
Yasumura
Y
Y
D000596:Amino Acids / Q000097:blood / Q000652:urine; D000818:Animals; D003433:Crosses, Genetic; D003605:D-Amino-Acid Oxidase / Q000172:deficiency*; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D000608:Renal Aminoacidurias / Q000652:urine*
D000596:Amino Acids; D003605:D-Amino-Acid Oxidase