Stimulation of the Ca2+-dependent polymerization of synexin by cis-unsaturated fatty acids.
The influence of fatty acids on the polymerization of synexin was studied by monitoring light scattered from solutions of purified synexin. Cis-unsaturated fatty acids such as arachidonate or oleate stimulated synexin polymerization at sub-micromolar concentrations, while saturated fatty acids, a trans unsaturated fatty acid or a fatty acid methyl ester had little effect. The polymerization of synexin occurred at lower concentrations of Ca2+ in the presence of the fatty acids than in the absence of fatty acids. Therefore, Ca2+ and free fatty acids may act as co-regulators of synexin action in stimulated secretory cells.
Sterner
D C
DC
Zaks
W J
WJ
Creutz
C E
CE
D000818:Animals; D017310:Annexin A7; D001704:Biopolymers; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008561:Membrane Fusion; D008968:Molecular Conformation; D009391:Nephelometry and Turbidimetry; D011506:Proteins / Q000378:metabolism*
D017310:Annexin A7; D001704:Biopolymers; D005231:Fatty Acids, Unsaturated; D011506:Proteins; D002118:Calcium