[Isolation and characteristics of a fraction of skeletal muscle sarcoplasmic reticulum enriched with terminal cistern vesicles].
A simple method for rapid isolation of active vesicles of sarcoplasmic reticulum is described. It is characterized by sensitivity to caffeine, high content of calsequestrin and the presence of 30 kDalton protein. The data obtained suggest that this fraction is enriched in vesicles derived from terminal cisterns.
Diadiusha
G P
GP
Kurskiĭ
M D
MD
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D002110:Caffeine / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000032:analysis / Q000201:enzymology / Q000648:ultrastructure
D002110:Caffeine; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases
Vydelenie i kharakteristika fraktsii sarkoplazmaticheskogo retikuluma skeletnykh myshts, obogashchennoĭ vezikulami terminal'nykh tsistern.