The pathways for fluid phase and receptor mediated endocytosis in rat hepatocytes are different but thermodynamically equivalent.
In isolated rat hepatocytes fluid phase endocytosis, determined by the uptake of the fluorescent dye lucifer yellow (LY), and receptor mediated endocytosis, determined using a ligand for the asialoglycoprotein receptor (asialo-orosomucoid; ASOR), are different pathways based on their different sensitivities to hyperosmolarity induced by sucrose (Oka and Weigel, J. Cell. Biol. 105, 311a, 1987). LY uptake was unaffected by 0.2 M sucrose at all temperatures tested between 12 degrees and 37 degrees C whereas the uptake of 125I-ASOR was completely inhibited at any temperature. Since the two probes are taken up by different pathways it was possible to determine independently the activation energies (Ea) for the fluid phase versus the receptor mediated coated pit endocytic process. The Ea was 26.4 +/- 3.5 and 25.8 +/- 1.9 kcal/mole for, respectively, receptor mediated and fluid phase endocytosis. These values are not significantly different, and we conclude that the fluid phase and receptor mediated pathways are thermodynamically equivalent even though they are independent.
Oka
J A
JA
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.
Weigel
P H
PH
D000818:Animals; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D004705:Endocytosis / Q000187:drug effects; D004734:Energy Metabolism; D007546:Isoquinolines; D008099:Liver / Q000378:metabolism*; D008297:Male; D009961:Orosomucoid / Q000031:analogs & derivatives* / Q000378:metabolism; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000187:drug effects / Q000502:physiology*; D013395:Sucrose; D013816:Thermodynamics
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D007546:Isoquinolines; D009961:Orosomucoid; D011971:Receptors, Immunologic; C017030:asialoorosomucoid; D013395:Sucrose; C017475:lucifer yellow