The influence of alpha-tocopherol and pyritinol on oxidative DNA damage and lipid peroxidation in human lymphocytes.
Unscheduled DNA synthesis (UDS) and lipid peroxidation (LPO) were measured in human peripheral lymphocytes from healthy volunteers. These processes were induced by the catalytic system Fe2+-sodium ascorbate. The degree of induced LPO was measured spectrophotometrically by the thiobarbituric acid assay. UDS was detected by scintillometric measurement of the incorporation of 3H-thymidine into DNA. The protective action by fat-soluble vitamin E (D,L-alpha-tocopherol) and the artificial antioxidant pyritinol on UDS and LPO was also investigated. The system Fe2+ (2 mumole/l)-sodium ascorbate (30 mumole/l) increased the LPO level in healthy volunteers approximately 2.5 times and the incorporation of 3H-thymidine by 60-70%. alpha-Tocopherol (0.2 mmole/l) very efficiently suppressed LPO processes (p less than 0.01) and the oxidative damage of DNA measured as UDS was also significantly diminished (p less than 0.05). Pyritinol had no effect on LPO and UDS under our experimental conditions.
Topinka
J
J
Psychiatric Research Institute, Prague, Czechoslovakia.
Binkova
B
B
Sram
R J
RJ
Erin
A N
AN
D001205:Ascorbic Acid / Q000494:pharmacology; D004260:DNA Repair; D006801:Humans; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000378:metabolism*; D008214:Lymphocytes / Q000502:physiology*; D011725:Pyridines / Q000494:pharmacology*; D011746:Pyrithioxin / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D008054:Lipid Peroxides; D011725:Pyridines; D014810:Vitamin E; D011746:Pyrithioxin; D001205:Ascorbic Acid