Polyclonal anti-idiotypes influence macrophage chemotaxis in coxsackievirus-induced murine myocarditis.
Investigation into the mechanism(s) whereby monocytes-macrophages are mobilized to the myocardium of coxsackievirus (CVB3)-infected mice was approached by assessing the role of anti-idiotypic antibodies against specific antiviral IgG molecules. Balb/c mice preinoculated with polyclonal anti-idiotypic, syngeneic antibodies resulted in significantly decreased myocarditis after virus challenge. On the other hand, oil-induced peritoneal exudate cells obtained from virus-exposed animals demonstrated increased chemotaxis in the presence of polyclonal anti-Id preparations, whereas peritoneal exudate cells from normal animals did not. Specificity of the anti-Ids as confirmed by the binding of the syngeneic anti-anti-idiotypic antibodies (antibody 3) to solubilized viral antigen(s) and Fab fragments prepared from the anti-idiotypic antibodies. Paradoxic biological effects of anti-Id, demonstrated in vitro and in vivo, suggest possible and subtle immunoregulation of the macrophage effector arm by anti-idiotypes during the course of virus infection.
Paque
R E
RE
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758.
Miller
R
R
D000818:Animals; D002633:Chemotaxis; D003384:Coxsackievirus Infections / Q000276:immunology*; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000276:immunology*
D007130:Immunoglobulin Idiotypes