Synthesis and secretion of proteins by postpartum human oviductal tissue in culture.
An explant culture system that used labelled leucine and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) with fluorography was used to identify specific de novo synthesized and released polypeptides by the human postpartum oviduct. Both ampulla and isthmus tissue in culture exhibited de novo synthesis and release of a large number of polypeptide subunits. Immunoglobulins A and G appear to be the major proteins produced in the ampulla. In addition, two complexes of acidic (pI less than 5) polypeptide subunits are found primarily in ampulla culture medium. Two families of proteins (Mr 51,000 and 60,000) are released by the isthmus but appear to be minor in the ampulla cultures.
Buhi
W C
WC
Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville 32610.
Van Wert
J W
JW
Alvarez
I M
IM
Dones-Smith
M M
MM
Bernhisel
M A
MA
D003470:Culture Media; D046508:Culture Techniques; D015180:Electrophoresis, Gel, Two-Dimensional; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007930:Leucine; D010779:Photofluorography; D049590:Postpartum Period / Q000378:metabolism*; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000378:metabolism
D003470:Culture Media; D011506:Proteins; D007930:Leucine