title: Restored invasion of mouse MO4 cells into chick heart in vitro through mutual conditioning at reduced temperature.
abstract: Invasion of malignant mouse MO4 cells into embryonic chick heart fragments in confronting organ cultures was arrested for 7 days when the temperature of incubation was lowered to 28 degrees C. Afterwards invasion resumed and progression between days 10 and 17 at 28 degrees C was comparable to that between days 0 and 7 at 37 degrees C. This pattern of progression of MO4 cell invasion at 28 degrees C was unaltered when either MO4 cells or heart fragments or both were preincubated separately at 28 degrees C for 14 days before confrontation with each other. Invasion at 28 degrees C resumed only when MO4 cells and heart tissue had been in immediate contact for at least 7 days. Metabolic labelling with [3H]fucose showed a correlation in time between transient suppression of invasion and transient inhibition of incorporation of fucosylation-precursor molecules into glycoproteins by MO4 cells. The latter activity was far less temperature-sensitive in heart cells. Our observations suggest that metabolic cooperation between invading MO4 cells and heart tissue is essential for progression of invasion in vitro.
authors.affiliation: Laboratory of Experimental Cancerology, University Hospital, Ghent, Belgium.
authors.forename: E A
authors.initials: EA
authors.lastname: Bruyneel
authors.forename: J G
authors.initials: JG
authors.lastname: Bolscher
authors.forename: L A
authors.initials: LA
authors.lastname: Smets
authors.forename: M
authors.initials: M
authors.lastname: De Mets
authors.forename: M M
authors.initials: MM
authors.lastname: Mareel
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement; D002642:Chick Embryo; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D006321:Heart / Q000196:embryology; D007700:Kinetics; D051379:Mice; D008954:Models, Biological; D009206:Myocardium / Q000166:cytology / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009924:Organ Culture Techniques; D012513:Sarcoma, Experimental / Q000473:pathology* / Q000503:physiopathology; D013696:Temperature
chemical_list: D006023:Glycoproteins; D005643:Fucose