title: Role of macrophages as modulators but not as autonomous accessory cells in primary antibody response.
abstract: The role of macrophages (M phi) in the in vitro primary antibody response of murine lymphocytes to sheep erythrocytes was investigated. Peritoneal M phi were activated to express Ia antigens either in vitro or in vivo. Nonactivated Ia- M phi were also examined. We observed that only Ia- M phi but also Ia+ M phi failed to trigger the antibody response, in contrast with splenic dendritic cells (DC) which served as potent and autonomous accessory cells, but that M phi modulated the level of response which was dependent primarily on the DC content of culture. The modulation appeared to incline to suppression rather than enhancement, when M phi were allowed to remain throughout the culture period for 4 days. A highly enhancing capacity of M phi, however, could be revealed by removing M phi 2 days after the initiation of culture, indicating that M phi exerted their suppressive effect more strongly in the late phase than in the early phase of in vitro antibody response. The modulatory activity seemed higher in Ia+ M phi than in Ia- M phi.
authors.forename: S
authors.initials: S
authors.lastname: Komatsubara
authors.forename: Y
authors.initials: Y
authors.lastname: Hirayama
authors.forename: K
authors.initials: K
authors.lastname: Inaba
authors.forename: K
authors.initials: K
authors.lastname: Naito
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: J
authors.initials: J
authors.lastname: Kawai
authors.forename: S
authors.initials: S
authors.lastname: Muramatsu
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000145:classification / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology
chemical_list: D001789:Blood Group Antigens