Solution conformation of sialosylcerebroside (GM4) and its NeuAc(alpha 2----3)Gal beta sugar component.
The solution conformations of GM4 ganglioside [NeuAc(alpha 2----3)Gal (beta 1----1)Cer] in (2H3C)2SO and its component disaccharide in 2H2O were investigated with the aid of 1H-nuclear magnetic resonance spectroscopy (nuclear Overhauser effect, analysis of coupling constants) and by energy-minimum calculations. The existence of three low-energy conformers obtained by theoretical calculations was supported by experimental findings in the case of GM4, whereas the disaccharide appears to exist as a mixture of two conformers.
Poppe
L
L
Max-Planck-Institut für Medizinische Forschung, Heidelberg, Federal Republic of Germany.
Dabrowski
J
J
von der Lieth
C W
CW
Numata
M
M
Ogawa
T
T
D002236:Carbohydrate Conformation; D004187:Disaccharides; D005690:Galactose; D005732:Gangliosides; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008968:Molecular Conformation; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids; D012996:Solutions; D013816:Thermodynamics
D004187:Disaccharides; D005732:Gangliosides; D012794:Sialic Acids; D012996:Solutions; C028253:ganglioside, GM4; D006859:Hydrogen; D019158:N-Acetylneuraminic Acid; D005690:Galactose