title: Enzyme-immunoassay for estrogen receptors in human pituitary adenomas.
abstract: We have performed an enzyme-immunoassay for estrogen receptor on 56 human pituitary adenomas and compared the results with a single point estradiol binding assay. There was a significant positive correlation between the two assays of cytoplasmic estrogen receptor (r = 0.960). Normal human pituitaries (N = 2) had an estrogen receptor concentration of 17 fmol/mg protein by enzyme-immunoassay. Of 14 prolactinomas, 6 (43%) contained estrogen receptor with a concentration of 33.5 +/- 7.4 (mean +/- SEM) fmol/mg protein. Six of 11 (55%) macroprolactinomas were estrogen receptor-positive, whereas all 3 microprolactinomas were estrogen receptor-negative. Only one (13%) of 8 GH- and PRL-secreting adenomas, and 3 of 6 (50%) gonadotropin-secreting adenomas were estrogen receptor-positive; the latter had a concentration of 13.5 +/- 1.6 fmol/mg protein. Estrogen receptor was not detected in 21 pure GH-secreting adenomas and 7 nonsecreting adenomas. These results demonstrate the precise frequency of estrogen receptor in various human pituitary adenomas, since enzyme-immunoassay as well as single point estradiol binding assay could detect estrogen receptor even in small specimens. Enzyme-immunoassay is suitable for evaluation of estrogen receptor status in human pituitary adenomas.
authors.affiliation: Third Department of Internal Medicine, Osaka University Hospital, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakao
authors.forename: M
authors.initials: M
authors.lastname: Koga
authors.forename: M
authors.initials: M
authors.lastname: Arao
authors.forename: M
authors.initials: M
authors.lastname: Nakao
authors.forename: B
authors.initials: B
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Kishimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Saitoh
authors.forename: N
authors.initials: N
authors.lastname: Arita
authors.forename: S
authors.initials: S
authors.lastname: Mori
mesh_terms: D000236:Adenoma / Q000032:analysis*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003600:Cytosol / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010911:Pituitary Neoplasms / Q000032:analysis*; D015175:Prolactinoma / Q000032:analysis*; D011959:Receptors, Estradiol / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis*
chemical_list: D011959:Receptors, Estradiol; D011960:Receptors, Estrogen