Serotonin (5-hydroxytryptamine) turnover in adult female Ascaris suum tissue.
1. The 5-hydroxytryptamine (5-HT, serotonin) turnover was examined in the tissues of adult female Ascaris suum. The 5-HT turnover was highest in the intestine at 34.7 ng 5-HT produced/mg protein/hr and 13.8 ng 5-HT produced/mg protein/hr in muscle tissue. 2. The levels of 5-HT metabolites namely tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine, 5-hydroxyindole acetic acid and 5-hydroxytryptophol were measured in muscle and intestinal tissue of adult A. suum. 3. Parachlorophenylalanine inhibited 5-HT production in muscle and intestinal tissue providing in situ evidence for the presence of tryptophan hydroxylase in this tissue. 4. Pargyline increased 5-HT production in muscle and intestinal tissue providing in situ evidence for the presence of monoamine oxidase in this tissue.
Martin
R E
RE
Department of Biological Sciences, University of North Texas, Denton 76203.
Chaudhuri
J
J
Donahue
M J
MJ
D000818:Animals; D001200:Ascaris / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D010477:Perfusion; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin