Presence of pepsinogens immunoreactive to anti-embryonic chicken pepsinogen antiserum in fish stomachs: possible ancestor molecules of chymosin of higher vertebrates.
1. Stomachs of adult teleosts and elasmobranchs reacted to an anti-embryonic chicken pepsinogen antiserum (anti-ECPg) as well as to an anti-adult chicken pepsinogen antiserum (anti-ACPg). 2. Zymograms and immunoblots of stomach extracts revealed that anti-ECPg- and anti-ACPg-reactive substances possess peptic activity. 3. The possible relationship between anti-ECPg-reactive pepsinogens in fish and prochymosins in higher vertebrates is discussed.
Yasugi
S
S
Zoological Institute, University of Tokyo, Japan.
Matsunaga
T
T
Mizuno
T
T
D000818:Animals; D002645:Chickens / Q000276:immunology*; D012085:Chymosin / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D015151:Immunoblotting; D007120:Immunochemistry; D008970:Molecular Weight; D010435:Pepsinogens / Q000032:analysis*
D007106:Immune Sera; D010435:Pepsinogens; D012085:Chymosin