title: Suppression of IgE synthesis in vitro by allogeneic T cells from atopic and non-atopic subjects.
abstract: The role of T cells in the regulation of IgE synthesis by human PBMC was studied. PBMC or separated and recombined populations of T and B cells from both normal and atopic donors were cultured for 10 days with and without cycloheximide. IgE and IgG synthesis were determined by specific RIA. IgE synthesis was detected in 0/30 non-atopic, 6/34 mildly atopic and 25/31 severely atopic subjects. Autologous T cells from 10/26 atopic donors, whose B cells synthesised IgE, significantly suppressed this IgE synthesis. The addition of allogeneic T cells from atopic or non-atopic subjects to atopic B cells resulted in greater suppression of IgE synthesis than the addition of autologous T cells. These data support the notion that atopic subjects have naturally occurring IgE isotype-specific suppressor T cells as well as suppressor T cells which can be activated during incubation with alloantigen.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zaunders
authors.forename: D A
authors.initials: DA
authors.lastname: Cooper
authors.forename: Y
authors.initials: Y
authors.lastname: Young
authors.forename: M
authors.initials: M
authors.lastname: Duckett
authors.forename: R
authors.initials: R
authors.lastname: Penny
authors.forename: J B
authors.initials: JB
authors.lastname: Ziegler
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007073:Immunoglobulin E