RA can alter the distribution of ATPase-positive Langerhans cells in the hamster cheek pouch in association with DMBA application.
Thirty-six young adult male and female Syrian hamsters were divided into four groups. Group 1 (N = 6) was treated with mineral oil, Group 2 (N = 12) was treated with 7,12-dimethylbenz[a]anthracene (DMBA), Group 3 (N = 12) was treated with DMBA and 13-cis-retinoic acid (RA), and Group 4 (N = 6) was treated with RA only. The hamsters were treated three times a week for six weeks. When the DMBA-treated animals were also treated with RA, tumor formation was inhibited. In the DMBA-treated animals that did not have RA, there was a significant reduction in the number of Langerhans cells (LCs) located interfocally and in foci compared with controls (p less than or equal to 0.001). The systemic administration of 10 mg of RA significantly increased the number of ATPase-positive LCs in the focal aggregates (p less than or equal to 0.001). Interfocally, the number of ATPase-positive LCs was statistically elevated in pouches from RA- and DMBA-treated animals (Group 2 vs. Group 3, p less than or equal to 0.01). Following the administration of RA (Group 4), there was a statistically significant increase of LCs interfocally compared with Groups 2 or 3 (p less than or equal to 0.001). RA administered alone resulted in a small increase in foci compared with Group 3 but in a significant decrease compared with the control group (p less than or equal to 0.001). Systemic RA appears to not only affect the number of ATPase-positive LCs but also to alter the distribution of these cells in DMBA-treated pouches.(ABSTRACT TRUNCATED AT 250 WORDS)
Schwartz
J L
JL
Frim
S R
SR
Shklar
G
G
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002452:Cell Count; D002610:Cheek; D006224:Cricetinae; D005260:Female; D015474:Isotretinoin; D007801:Langerhans Cells / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects* / Q000201:enzymology; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin; D015127:9,10-Dimethyl-1,2-benzanthracene; D000251:Adenosine Triphosphatases; D015474:Isotretinoin