title: Effect of subminimal inhibitory concentrations of pefloxacin on the piliation and adherence of E. coli.
abstract: Several recent reports have shown that sub-lethal concentrations of antibiotics may decrease the adhesive ability of bacteria to epithelial cells; however, the mechanism by which the antimicrobial agents reduce adherence has remained unknown. The effect of sub-MICs of pefloxacin, a new broad-spectrum antibacterial quinolone, was studied on: haemagglutination, adherence, outer membrane and fimbriae of a pyelonephritogenic E. coli strain. The strain agglutinated in a mannose-resistant way human P1 but not p erythrocytes. After purification, the fractions containing outer membrane and fimbriae proteins were studied using electron microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The E. coli strain, grown in the presence of sub-MICs of pefloxacin, exhibited: (i) abnormal forms with filament formation; (ii) a markedly altered capacity of haemagglutination (human P1 erythrocytes) and adherence to uroepithelial cells. The inhibition of haemagglutination and adherence corresponded to a decrease in P-fimbriae production. These results were also associated with changes in the extraction of the outer membrane proteins of pefloxacin-treated bacteria. No major qualitative difference could be seen in outer membrane and P-fimbriae protein profiles after growth with sub-MICs of pefloxacin. This study has demonstrated that sub-lethal concentrations of pefloxacin alter piliation and the outer membrane of E. coli. These changes are associated with reduced bacterial haemagglutination and adherence.
authors.affiliation: Rhône-Poulenc Santé, Institute of Biopharmacy, Antony, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Desnottes
authors.forename: D
authors.initials: D
authors.lastname: Le Roy
authors.forename: N
authors.initials: N
authors.lastname: Diallo
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure*; D010861:Fimbriae, Bacterial / Q000187:drug effects / Q000648:ultrastructure*; D006384:Hemagglutination / Q000187:drug effects; D008854:Microscopy, Electron; D015366:Pefloxacin / Q000494:pharmacology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D015366:Pefloxacin