title: Regulation of colony formation of differentiated chondrocytes in soft agar by transforming growth factor-beta.
abstract: Fibroblast growth factor (FGF) induces colony formation by chondrocytes in soft agar (Y. Kato et al., J. Cell. Physiol., 1987), and the present study revealed that transforming growth factor-beta(TGF-beta) does not induce the same effect. TGF-beta did, however, increase the efficiency of colony formation by chondrocytes 3- to 4-fold in the presence of a maximal dose of FGF. Furthermore, TGF-beta decreased the concentrations of FGF needed for the induction of cell growth in soft agar by 40- to 100-fold. These results suggest that TGF-beta is involved in the control of cartilage growth possibly by increasing the responsiveness of chondrocytes to FGF.
authors.affiliation: Department of Biochemistry, Faculty of Denistry, Osaka University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Iwamoto
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
authors.forename: H
authors.initials: H
authors.lastname: Fuchihata
authors.forename: F
authors.initials: F
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003114:Colony-Forming Units Assay; D004247:DNA / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D007700:Kinetics; D011817:Rabbits; D015291:Transforming Growth Factors / Q000494:pharmacology*
chemical_list: D005346:Fibroblast Growth Factors; D015291:Transforming Growth Factors; D003094:Collagen; D004247:DNA