Synthesis of the major inducible heat shock protein in rat hippocampus after neonatal hypoxia-ischemia.
Rats aged 7 days were exposed to 3.5 h of cerebral hypoxia-ischemia produced by unilateral common carotid artery ligation combined with hypoxia (8% oxygen). The major inducible heat-shock protein, HSP-68, was synthesized in ipsilateral but not contralateral (control) hippocampus during early recovery (1 and 3 h). HSP-68 synthesis was not detected during longer recovery periods. The presence of HSP-68 was confirmed by Western blotting and immunostaining with a polyclonal antibody to HSP-68.
Dwyer
B E
BE
Epilepsy Research Laboratory, VA Medical Center, Sepulveda, California 91343.
Nishimura
R N
RN
Brown
I R
IR
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D002339:Carotid Arteries / Q000502:physiology; D007839:Functional Laterality; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006624:Hippocampus / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013462:Sulfur Radioisotopes
D006360:Heat-Shock Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine