title: Incidence of chromosomal anomalies in early bovine embryos derived from in vitro fertilization.
abstract: The incidence of chromosomal anomalies in early bovine embryos derived from follicular oocytes fertilized in vitro using sperm separated by Percoll density gradient centrifugation was investigated. Overall, chromosomal anomalies were observed in 13.7% (138/1005) of embryos. There were 14 haploids (1.4%), 2 hypodiploids (0.2%), 6 hyperdiploids (0.6%), 101 triploids (10.0%), 12 tetraploids (1.2%), 2 diploid/triploid mosaics (0.2%), and 1 diploid/tetraploid mosaic (0.1%). The frequency of triploidy was caused mainly by polyspermy. There was a significant difference in the frequency of embryos with abnormal chromosomes between the two bulls used (P less than 0.005), but Percoll centrifugation did not affect the observed incidence of anomalies. The frequency of chromosomal anomalies in embryos at each stage increased with delay or arrest of development. These results suggest that the incidence of chromosomal anomalies depended on the conditions of in vitro fertilization and the arrest of development.
authors.affiliation: NODAI Research Institute, Tokyo University of Agriculture, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Iwasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Shioya
authors.forename: H
authors.initials: H
authors.lastname: Masuda
authors.forename: A
authors.initials: A
authors.lastname: Hanada
authors.forename: T
authors.initials: T
authors.lastname: Nakahara
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002417:Cattle; D002469:Cell Separation; D002869:Chromosome Aberrations; D004622:Embryo, Mammalian / Q000648:ultrastructure; D005307:Fertilization in Vitro