title: Characterization of different forms of dipeptidyl peptidase IV from rat liver and hepatoma by monoclonal antibodies.
abstract: Nine monoclonal antibodies directed against DPP IV from rat liver plasma membranes were obtained. They recognized four different epitopes (A, B, C and D) of the enzyme. The epitopes A, B and C were located on the outside of the hepatocyte plasma membrane and were shared by DPP IV from hepatoma plasma membrane and the soluble form. Epitope D appeared to be partly inserted in the membrane and was found exclusively in the liver. Epitopes A, B and C and DPP IV revealed by histochemical means showed similar distribution patterns on frozen sections of various rat tissues, while epitope D did not show such a correlation. DPP IV is inserted in liver plasma membrane by two different mechanisms, one being phospholipase C-sensitive, while in hepatoma the enzyme is anchored in this membrane by a phospholipase C-resistant mechanism only.
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freien Universität, Berlin.
authors.forename: S
authors.initials: S
authors.lastname: Hartel
authors.forename: C
authors.initials: C
authors.lastname: Hanski
authors.forename: R
authors.initials: R
authors.lastname: Neumeier
authors.forename: R
authors.initials: R
authors.lastname: Gossrau
authors.forename: W
authors.initials: W
authors.lastname: Reutter
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4