title: Characterization of fimbrial subunits from Bordetella species.
abstract: Using antisera raised against serotype 2 and 3 fimbrial subunits from Bordetella pertussis, serologically related polypeptides were detected in Bordetella bronchiseptica, Bordetella parapertussis and Bordetella avium strains. The two B. pertussis fimbrial subunits, and three of the serologically related B. bronchiseptica polypeptides, were shown to be very similar in amino acid composition and N-terminal amino acid sequence. Homology was observed between the N-termini of these polypeptides, and fimbrial subunits from Escherichia coli, Haemophilus influenzae and Proteus mirabilis. A synthetic oligonucleotide probe, derived from the N-terminal sequence of the B. pertussis serotype 2 fimbrial subunit, was used to identify fimbrial genes in genomic Southern blots. The results suggested the presence of multiple fimbrial subunit genes in B. pertussis, B. bronchiseptica and B. parapertussis. The DNA probe was used to clone one of the three tentative fimbrial subunit genes detected in B. pertussis.
authors.affiliation: Laboratory for Bacteriology, Rijksinstituut voor Volksgezondheid en Milieuhygiëne, Bilthoven, The Netherlands.
authors.forename: F R
authors.initials: FR
authors.lastname: Mooi
authors.forename: H G
authors.initials: HG
authors.lastname: van der Heide
authors.forename: A R
authors.initials: AR
authors.lastname: ter Avest
authors.forename: K G
authors.initials: KG
authors.lastname: Welinder
authors.forename: I
authors.initials: I
authors.lastname: Livey
authors.forename: B A
authors.initials: BA
authors.lastname: van der Zeijst
authors.forename: W
authors.initials: W
authors.lastname: Gaastra
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D001884:Bordetella / Q000145:classification* / Q000235:genetics; D001886:Bordetella pertussis / Q000145:classification / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000032:analysis*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012703:Serotyping
chemical_list: D001426:Bacterial Proteins; D046911:Macromolecular Substances