Alterations in the sialylation and sulfation of secreted mouse thyrotropin in primary hypothyroidism.
We investigated the effect of in vivo hypothyroidism on the sialylation and sulfation of thyroid-stimulating hormone (TSH) secreted by mouse pituitary explants. Oligosaccharides from secreted thyroid-stimulating hormone from hypothyroid animals contained greater sialic acid relative to sulfate in both alpha and beta subunits. Aging per se had little effect on thyroid-stimulating hormone sialylation or sulfation. Variable sialylation and sulfation demonstrates a mechanism for charge microheterogeneity of thyroid-stimulating hormone, and the increasing sialylation observed with hypothyroidism may functionally mediate the prolonged metabolic clearance that has been noted previously.
DeCherney
G S
GS
Molecular, Cellular, and Nutritional Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Gesundheit
N
N
Gyves
P W
PW
Showalter
C R
CR
Weintraub
B D
BD
D000375:Aging; D000818:Animals; D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D007037:Hypothyroidism / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009844:Oligosaccharides / Q000378:metabolism; D012794:Sialic Acids / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*; D013972:Thyrotropin / Q000378:metabolism*
D009844:Oligosaccharides; D012794:Sialic Acids; D013431:Sulfates; D013972:Thyrotropin