Evaluation of equilibrium constants from precipitin curves: interaction of alpha-crystallin with an elicited monoclonal antibody.
A simple procedure, based on the precipitin curve and the antibody:antigen ratios of the precipitates, is described for evaluation of the intrinsic association constant (k) governing the interaction between a multivalent antigen and a bivalent antibody. Its application is illustrated with a study of the interaction between alpha-crystallin and an elicited monoclonal antibody, which is shown to exhibit essentially identical affinities (k = 9 X 10(4) M-1) for the alpha m and alpha c forms of the antigen.
Winzor
D J
DJ
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Stevens
A
A
Augusteyn
R C
RC
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis*; D002417:Cattle; D003459:Crystallins / Q000032:analysis* / Q000276:immunology; D007700:Kinetics; D007908:Lens, Crystalline; D011233:Precipitin Tests / Q000379:methods
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D003459:Crystallins