title: Ramoplanin (A-16686), a new glycolipodepsipeptide antibiotic. IV. Complete sequence determination by homonuclear 2D NMR spectroscopy.
abstract: Homonuclear 2D NMR spectroscopy double quantum filter correlation spectroscopy (DQF-COSY), relayed-COSY, nuclear Overhauser enhancement spectroscopy (NOESY), and DQF-relayed-NOESY) allowed the complete determination of the core depsipeptide of antibiotic ramoplanin (A-16686). In particular, the DQF-relayed-NOESY experiments were essential in assigning the single signals close to the diagonal.
authors.affiliation: Merrell Dow Research Institute-Lepetit Research Center, Gerenzano, Italy.
authors.forename: J K
authors.initials: JK
authors.lastname: Kettenring
authors.forename: R
authors.initials: R
authors.lastname: Ciabatti
authors.forename: G
authors.initials: G
authors.lastname: Winters
authors.forename: G
authors.initials: G
authors.lastname: Tamborini
authors.forename: B
authors.initials: B
authors.lastname: Cavalleri
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides; D006020:Glycopeptides / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000032:analysis; D010456:Peptides, Cyclic / Q000032:analysis; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D047630:Depsipeptides; D006020:Glycopeptides; D010446:Peptide Fragments; D010456:Peptides, Cyclic; C041438:ramoplanin