Indirect enzyme-linked immunosorbent assay for the mycotoxin zearalenone.
A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of zearalenone, an estrogenic mycotoxin. Zearalenone was converted to zearalenone-6'-carboxymethyloxime and conjugated to bovine serum albumin and poly-L-lysine for use as immunogen and solid-phase marker, respectively. Immunization of rabbits with the bovine serum albumin conjugate resulted in zearalenone antibody titers of 20,480 in 11 weeks. A competitive indirect ELISA was conducted by simultaneously incubating zearalenone with zearalenone antiserum over zearalenone-6'-carboxymethyloxime poly-L-lysine solid phase and then determining the bound rabbit immunoglobulin with goat anti-rabbit peroxidase conjugate. Response range for zearalenone in the resulting competition curve was between 1 and 50 ng/ml. Reactivities of this antiserum for alpha-zearalenol, beta-zearalenol, alpha-zearalanol, and beta-zearalanol were, respectively, 50, 12, 6, and 3% of that found for zearalenone. By using the competitive indirect ELISA, zearalenone was detectable in methanol-water extracts of corn, wheat, and pig feed samples.
Liu
M T
MT
Ram
B P
BP
Hart
L P
LP
Pestka
J J
JJ
D000821:Animal Feed; D000818:Animals; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005516:Food Microbiology; D007106:Immune Sera; D008892:Milk; D012118:Resorcinols / Q000032:analysis*; D014908:Triticum; D003313:Zea mays; D015025:Zearalenone / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D007106:Immune Sera; D012118:Resorcinols; D015025:Zearalenone