Palytoxin induces a relatively non-selective cation permeability in frog sciatic nerve which can be inhibited by cardiac glycosides.
The effect of palytoxin (PTX) on compound resting potential and compound action potential of frog sciatic nerve was studied using the sucrose-gap technique. PTX irreversibly depolarized the compound resting potential and reduced the amplitude of the compound action potential. PTX evoked a marked depolarization when extracellular Na+ was replaced by Li+, Cs+ and the organic cations methylammonium, hydroxylammonium, and methylhydroxylammonium but not by tetramethylammonium, tetraethylammonium, choline or the divalent cations, Ca2+ and Ba2+. The maintained depolarization was not sensitive to inhibition by saxitoxin (300 nM) or procaine (10 mM). The depolarization was inhibited by ouabain or cymarin but not by the aglycon, strophanthidin. However, strophanthidin did antagonize the inhibitory action of cymarin which suggests that PTX and cardiac glycosides do not share an identical binding site but there may be some overlap. We conclude that in frog sciatic nerve, PTX interacts with the (Na+-K+) pump to induce the opening or formation of a relatively non-selective cation pore within or near the pump protein.
Castle
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Harvard Medical School, Brigham and Women's Hospital, Department of Anesthesia Research Laboratories, Boston, MA 02115.
Strichartz
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GR
D000178:Acrylamides; D000818:Animals; D002301:Cardiac Glycosides / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003064:Cnidarian Venoms / Q000633:toxicity*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D011188:Potassium / Q000378:metabolism*; D011894:Rana pipiens; D012584:Sciatic Nerve / Q000187:drug effects*; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D000095702:Polyether Toxins
D000178:Acrylamides; D002301:Cardiac Glycosides; D003064:Cnidarian Venoms; D011188:Potassium; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; C010272:palytoxin; D000095702:Polyether Toxins