Staurosporine, a protein kinase C inhibitor, attenuates Ca2+-dependent stretch-induced vascular tone.
The effects of protein kinase C inhibition by staurosporine was studied on Ca-dependent tone of the rabbit facial vein. Tone was produced either by stretch or by readmission of Ca2+ in a non-depolarizing Ca2+-free salt solution. Stretch-induced tone was inhibited by staurosporine. When tissues were incubated in a Ca2+-free solution, staurosporine (50 nM) inhibited the contractile responses produced by readmission of Ca2+. These observations suggest that maintenance of stretch-induced extracellular Ca2+-dependent tone may be regulated by protein kinase C.
Laher
I
I
Department of Pharmacology, University of Vermont, Burlington 05405.
Bevan
J A
JA
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011817:Rabbits; D012016:Reference Values; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects*; D014680:Veins / Q000187:drug effects / Q000502:physiology
D000470:Alkaloids; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium