Soluble, nitrate/nitrite-inducible cytochrome P-450 of the fungus, Fusarium oxysporum.
Both soluble and microsomal fractions of Fusarium oxysporum contain cytochrome P-450(P-450). We report here that the P-450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal P-450 was synthesized regardless of the medium compositions. The reduced-CO complex of the soluble P-450 exhibited an absorption spectrum that is different from that of the microsomal counterpart. These results indicate that the soluble P-450 is distinct from the microsomal species and suggest a novel function for the former P-450.
Shoun
H
H
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Suyama
W
W
Yasui
T
T
D002248:Carbon Monoxide / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D005670:Fusarium / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D009566:Nitrates / Q000494:pharmacology*; D009573:Nitrites / Q000494:pharmacology*; D012977:Sodium Nitrite / Q000494:pharmacology*; D012995:Solubility; D013053:Spectrophotometry
D009566:Nitrates; D009573:Nitrites; D002248:Carbon Monoxide; C031618:sodium nitrate; D003577:Cytochrome P-450 Enzyme System; D012977:Sodium Nitrite