title: Duck hepatitis B virus: cloning and subcloning of the viral genome.
abstract: In the course of studies on the biology of hepadnavirus infections, duck hepatitis B virus (DHBV) DNA was isolated from the serum of a German Pekin duck. Viral DNA was cloned in E. coli using pBR 322 DNA as a vector. The cloned DHBV DNA F 1-6 was characterised by restriction enzyme analyses. DHBV DNA F 1-6 was subcloned in both orientations in plasmid pSP 65 to produce strand-specific RNA probes. These probes specifically identified asymmetrically replicating nascent minus-strand DHBV DNA species or plus-strand viral RNA transcripts.
authors.affiliation: Department of Medicine, University of Freiburg.
authors.forename: K
authors.initials: K
authors.lastname: Oexle
authors.forename: H E
authors.initials: HE
authors.lastname: Blum
authors.forename: E
authors.initials: E
authors.lastname: Walter
authors.forename: W B
authors.initials: WB
authors.lastname: Offensperger
authors.forename: S
authors.initials: S
authors.lastname: Offensperger
authors.forename: H
authors.initials: H
authors.lastname: Grundmann
authors.forename: K
authors.initials: K
authors.lastname: Teubner
authors.forename: W
authors.initials: W
authors.lastname: Gerok
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004372:Ducks; D005814:Genes, Viral; D005822:Genetic Vectors; D006509:Hepatitis B / Q000382:microbiology / Q000662:veterinary*; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011201:Poultry Diseases / Q000382:microbiology*; D015347:RNA Probes; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D015347:RNA Probes; D012367:RNA, Viral