Detection and characterization of a nucleoside transport system in human fibroblast lysosomes.
Lysosomes contain enzymatic activities capable of degrading nucleic acids to their constituent nucleosides, but the manner by which these degradation products are released from the lysosome is unknown. To investigate this process, human fibroblast lysosomes, purified on Percoll density gradients, were incubated with [3H]adenosine at pH 7.0, and the amount of adenosine taken up by the lysosomes was measured. Adenosine uptake by fibroblast lysosomes attained a steady state by 12 min at 37 degrees C and was unaffected by the presence of 2 mM MgATP or changes in pH from 5.0 to 8.0. An Arrhenius plot was linear with an activation energy of 12.9 kcal/mol and a Q10 of 2.0. Lysosomal adenosine uptake is saturable, displaying a Km of 9 mM at pH 7.0 and 37 degrees C. Various nucleosides and the nucleobase, 6-dimethylaminopurine, strongly inhibit lysosomal adenosine uptake, whereas neither D-ribose or nucleotide monophosphates have any significant effect upon lysosomal adenosine uptake. On a molar basis, purines are recognized more strongly than pyrimidines. Changing the nature of the nucleoside sugar from ribose to arabinose or deoxyribose has little effect on reactivity with this transport system. The known plasma membrane nucleoside transport inhibitors, dipyridamole and nitrobenzylthioinosine, inhibit lysosomal nucleoside transport at relatively low concentrations (25 microM) relative to the Km of 9 mM for lysosomal adenosine uptake. The half-times of [3H]inosine and [3H]uridine efflux from fibroblast lysosomes ranged from 6 to 8 min at 37 degrees C. Trans effects were not observed to be associated with either inosine or uridine exodus. In contrast to adenosine uptake, adenine primarily enters fibroblast lysosomes by a route not saturable by high concentrations of various nucleosides. In conclusion, the saturability of lysosomal adenosine uptake and its specific, competitive inhibition by other nucleosides indicate the existence of a carrier-mediated transport system for nucleosides within fibroblast lysosomal membranes.
Pisoni
R L
RL
Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor 48109-2029.
Thoene
J G
JG
D000225:Adenine / Q000378:metabolism; D000241:Adenosine / Q000378:metabolism; D026905:Amino Acid Transport Systems; D001692:Biological Transport; D002352:Carrier Proteins / Q000302:isolation & purification*; D002460:Cell Line; D004176:Dipyridamole; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism*; D009705:Nucleosides / Q000378:metabolism*; D013379:Substrate Specificity; D013868:Thioinosine / Q000031:analogs & derivatives
D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D009705:Nucleosides; D013868:Thioinosine; D004176:Dipyridamole; C001789:4-nitrobenzylthioinosine; D000225:Adenine; D000241:Adenosine