Effect of age on endogenous DNA single-strand breakage, strand break induction and repair in the adult housefly, Musca domestica.
It has been suggested that genomic alterations involving DNA damage and the ability to repair such damage play an important role in cellular senescence. In this study, endogenous DNA single-strand breaks, the susceptibility of DNA to induced strand breakage and the capacity to repair these breaks were compared in postmitotic cells from young (3-day-old) and old (23-day-old) houseflies. DNA single-strand breaks did not accumulate during normal aging in the housefly. However, cells of the old flies exhibited a greater sensitivity to single-strand breakage induced by gamma-radiation and UV light. The capacity to repair these exogenously induced single-strand breaks declined with age. Results do not support the view that DNA single-strand breaks are a causal factor in aging in the housefly. An age-related increase in the susceptibility to undergo single-strand breakage suggests alterations in chromatin during the aging process.
Newton
R K
RK
Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275.
Ducore
J M
JM
Sohal
R S
RS
D000375:Aging; D000818:Animals; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004306:Dose-Response Relationship, Immunologic; D005720:Gamma Rays; D006793:Houseflies / Q000235:genetics*; D014466:Ultraviolet Rays
D004247:DNA