Characterization with monoclonal and polyclonal antibodies of a new major allergen from grass pollen in the group I molecular weight range.
We have purified a 24/25 kd allergen from orchard grass pollen (Dactylis glomerata) that has an allergenic potency similar to that of the major group I allergen. We provisionally named this allergen grass 4B1 after the monoclonal antibody used for its identification and purification. This monoclonal antibody was obtained by immunizing mice with whole Lolium perenne-pollen extract and by screening the antibody producing hybrids for reactivity with Dactylis glomerata-pollen extract. Grass 4B1 is physicochemically separable from the group I allergen. Polyclonal rabbit antibodies to grass 4B1 do not react with group I allergen or vice versa. Ninety-five sera with IgE antibodies to grass pollen were tested for IgE antibodies to grass 4B1, and greater than 90% was positive in this test. The median response to grass 4B1 was 70% of that to Lol p I.
van Ree
R
R
Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Clemens
J G
JG
Aalbers
M
M
Stapel
S O
SO
Aalberse
R C
RC
D000485:Allergens; D000818:Animals; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D006801:Humans; D007106:Immune Sera; D007073:Immunoglobulin E; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010940:Plant Proteins; D006109:Poaceae / Q000276:immunology*; D011058:Pollen / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D011852:Radioallergosorbent Test
D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C057603:Dac g I protein, Dactylis glomerata; D007106:Immune Sera; D007074:Immunoglobulin G; D010940:Plant Proteins; D007073:Immunoglobulin E