title: Ribitol dehydrogenase of Klebsiella aerogenes. Sequence of the structural gene.
abstract: The ribitol dehydrogenase gene was cloned from wild-type Klebsiella aerogenes and also from a transducing phage lambda prbt which expresses the rbt operon constitutively. The coding sequence for 249 amino acids is separated from the following D-ribulokinase gene by 31 base pairs containing three stop codons, one of which overlaps the ribosome binding site for D-ribulokinase. Three residues in the amino acid sequence differ from that predicted from the DNA sequence: Asp-212 for Asn-212 is probably a protein sequencing error, but -Ala-Val- for -Ser-Ser- at 146-147 appears to be a 'neutral mutation' that may have arisen during prolonged chemostat selection of a strain that superproduces the enzyme from which the protein sequence was determined.
authors.forename: T
authors.initials: T
authors.lastname: Loviny
authors.forename: P M
authors.initials: PM
authors.lastname: Norton
authors.forename: B S
authors.initials: BS
authors.lastname: Hartley
mesh_terms: D010582:Bacteriophage lambda; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005796:Genes; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D009876:Operon; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D013401:Sugar Alcohol Dehydrogenases; C022529:ribitol 2-dehydrogenase