Anti-OTF-1 antibodies inhibit NFIII stimulation of in vitro adenovirus DNA replication.
HeLa cell OTF-1 has been purified on the basis of its DNA binding activity and used to raise a polyclonal rabbit antiserum. This antiserum is shown to recognize both native and denatured OTF-1 from both human and a similar protein from Xenopus culture cells, but to react either more weakly or not at all with the lymphoid cell-specific OTF-2. Separately, NFIII has been purified on the basis of its ability to stimulate Adenovirus DNA replication in vitro. On denaturing polyacrylamide gels OTF-1 and NFIII exhibit identical mobility. Anti-OTF-1 antiserum recognizes NFIII and neutralizes its stimulatory effect on DNA replication. Moreover, OTF-1 can functionally replace NFIII. Taken together with previously published DNA binding data, this indicates that OTF-1 and NFIII are either very closely related or identical.
Pruijn
J M
JM
Laboratory for Physiological Chemistry, State University of Utrecht, The Netherlands.
van der Vliet
P C
PC
Dathan
N A
NA
Mattaj
I W
IW
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D000906:Antibodies / Q000502:physiology*; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D004279:DNA, Viral / Q000096:biosynthesis*; D006367:HeLa Cells; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D009687:Nuclear Proteins / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D014157:Transcription Factors / Q000276:immunology* / Q000302:isolation & purification; D014981:Xenopus
D000906:Antibodies; D004279:DNA, Viral; D007106:Immune Sera; D009687:Nuclear Proteins; D014157:Transcription Factors