African swine fever virus-induced proteins on the plasma membranes of infected cells.
The African swine fever virus-induced proteins on plasma membranes of infected cells have been studied by two different procedures, iodination and incubation of infected cells labeled with [35S]methionine with a specific antiserum, obtained from pigs immunized with a monkey stable cell-adapted African swine fever virus. The combined use of both procedures identified proteins IP56, IP51, IP35, IP34, IP31, IP30, IP25.5, IP23.5, IP16, IP15, IP14, and IP12 as viral antigens exposed on the surface of infected cells. Proteins IP16, IP15, and IP14 were recognized by the immune serum from survivor pigs, obtained after challenge with homologous virulent virus, but not by the immune serum from the same pigs immunized only with the cell-adapted virus.
Alcaraz
C
C
Departamento de Virología Animal, Instituto Nacional de Investigaciones Agrarias, Madrid, Spain.
Pasamontes
B
B
Ruiz Gonzalvo
F
F
Escribano
J M
JM
D000358:African Swine Fever Virus / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis* / Q000276:immunology; D002478:Cells, Cultured; D007106:Immune Sera / Q000276:immunology; D007497:Iridoviridae / Q000378:metabolism*; D008264:Macrophages / Q000032:analysis / Q000382:microbiology*; D013552:Swine; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology
D000956:Antigens, Viral; D007106:Immune Sera; D014764:Viral Proteins