title: Antibodies raised against synthetic peptides from the Arg-Gly-Asp-containing region of the Leishmania surface protein gp63 cross-react with human C3 and interfere with gp63-mediated binding to macrophages.
abstract: The Leishmania surface glycoprotein gp63 binds to complement receptor type 3 on the macrophage surface. Antibody raised against a synthetic peptide containing the Arg-Gly-Asp region of the amino acid sequence of gp63 recognizes both gp63 and the alpha-chain of human C3. Monovalent Fab fragments from this antibody block gp63-mediated binding to macrophages.
authors.affiliation: Department of Pathology, New York University Medical Center 10016.
authors.forename: D G
authors.initials: DG
authors.lastname: Russell
authors.forename: P
authors.initials: P
authors.lastname: Talamas-Rohana
authors.forename: J
authors.initials: J
authors.lastname: Zelechowski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003176:Complement C3 / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D007894:Leishmania mexicana / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D003176:Complement C3; D008562:Membrane Glycoproteins; D010446:Peptide Fragments