Purification of fimbriae from Pasteurella haemolytica A-1.
Pasteurella haemolytica A-1 produces large, rigid fimbriae with a diameter of 12 nm and a denisty of 1.32. Their subunit molecular weight was 35,000 as determined by SDS-polyacrylamide gel electrophoresis and immunoblotting with monoclonal antibodies raised against native fimbriae. The isoelectric point of the purified fimbriae was 4.8. While Pasteurella haemolytica whole cells plus a crude shear fraction were capable of agglutinating bovine erythrocytes, purified fimbriae exhibited no such activity.
Potter
A A
AA
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Ready
K
K
Gilchrist
J
J
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D010861:Fimbriae, Bacterial / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D006384:Hemagglutination; D015151:Immunoblotting; D007525:Isoelectric Focusing; D008854:Microscopy, Electron; D010325:Pasteurella / Q000648:ultrastructure*