Reaction of toxic shock syndrome toxin 1 with endothelium of human umbilical cord vein.
The binding of toxic shock syndrome toxin 1 (TSST-1) to the endothelium of human umbilical cord veins and arteries was studied. Radiolabeled TSST-1, perfused through a segment of human umbilical cord vein, bound to the vessel at 4 degrees C, with a dissociation constant (Kd) of 6.5 X 10(-10) M. A pre-embedding electron-microscopic immunogold technique revealed that TSST-1 was located in the veins and arteries on the surface of endothelial cells and in the area of cell contacts. Umbilical cord vein segments filled with TSST-1 were incubated for 30 minutes at 37 degrees C, fixed by perfusion and embedded in hydrophilic resin (Lowicryl K4M). Postembedding immunogold staining of ultrathin sections showed TSST-1 in the vesicles crossing the cytoplasm of the veins' endothelial cells and entering underlying elastic and collagen layers.
Kushnaryov
V M
VM
Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226.
MacDonald
H S
HS
Reiser
R F
RF
Bergdoll
M S
MS
D001427:Bacterial Toxins; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D004768:Enterotoxins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D018089:Superantigens; D014471:Umbilical Veins / Q000378:metabolism / Q000648:ultrastructure
D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal