The immunocytochemical preservation of IgE and mast cells of the rat.
The localisation of IgE to mast cells of rats infested with Nippostrongylus brasiliensis has been studied in an attempt to find a fixation procedure which will best preserve (a) the antigenicity of IgE for immunocytochemical demonstration, and (b) the histochemical staining properties of mast cells. A fixative comprising Carnoy's fluid and picric acid (CPA) best fulfilled these criteria, allowing localisation of IgE to both the plasma membrane and cytoplasm of mucosal mast cells of rat small intestine, but only to the plasma membrane of connective tissue mast cells of rat tongue. Formaldehyde-containing fixatives prevented labelling of IgE in the cytoplasm of mucosal mast cells.
Hickling
K C
KC
Department of Pathology, Fisons Pharmaceuticals, R & D Laboratories, Loughborough, U.K.
Greenwood
B
B
D000818:Animals; D002451:Cell Compartmentation; D002463:Cell Membrane Permeability; D005404:Fixatives; D007073:Immunoglobulin E / Q000378:metabolism*; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000166:cytology; D008407:Mast Cells / Q000276:immunology*; D051381:Rats; D014059:Tongue / Q000166:cytology
D005404:Fixatives; D007073:Immunoglobulin E