[Evaluation of the expression of damage of the DNA-structural complex of rat thymocytes based on kinetics of alkaline lysis following irradiation].
Rotary viscosimeters were used to study the postirradiation destruction of the DNA-structural complex (DSC) of rat thymocyte nuclei exhibited by a change in alkaline denaturation of DSC upon lysis. The S area, limited by the characteristic viscosity values obtained during alkaline lysis of thymocyte nuclei, was used as a characteristic of DSC. Immediately after irradiation the S area changed up to 81-84 per cent at 0.5-1.5 Gy and up to 56-44 per cent at 2-10 Gy. 6 to 24 h following irradiation a change in the profile of alkaline denaturation of DSC was a function of dose and dropped from 100 down to 11 per cent at doses of 0 to 10 Gy. After 2-3 days, the changes in S were also observed but they were not a strict function of dose and were the same with the values obtained immediately after irradiation.
Rusinova
G G
GG
Turdakova
V A
VA
D000818:Animals; D003037:Cobalt Radioisotopes; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D005720:Gamma Rays; D008297:Male; D009691:Nucleic Acid Denaturation; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000528:radiation effects*
D003037:Cobalt Radioisotopes; D004247:DNA
Otsenka vyrazhennosti narusheniĭ DNK-strukturnogo kompleksa timotsitov krys po kinetike shchelochnogo lizisa posle radiatsionnogo vozdeĭstviia.