Synthesis and characterization of fluorescent Lucifer yellow-lipid conjugates.
The syntheses of fluorescent lipid probes composed of Lucifer yellow dyes linked to either cholesterol or phospholipids are described. The spectral properties of these probes are characterized, and the probes are evaluated for use with model membranes and with live animal and plant cells. Of the probes synthesized, the cholesterol derivative is the easiest to prepare and appears to be the most useful because it readily labels the plasma membrane of live cells and maintains a high ratio of cell surface-to-cytoplasmic fluorescence.
Nothnagel
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Department of Botany and Plant Sciences, University of California, Riverside 92521.
D000818:Animals; D002462:Cell Membrane; D002736:Chloroplasts / Q000032:analysis; D002784:Cholesterol; D004910:Erythrocyte Membrane / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis*; D007546:Isoquinolines; D008563:Membrane Lipids / Q000032:analysis; D015394:Molecular Structure; D010743:Phospholipids; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007546:Isoquinolines; D008563:Membrane Lipids; D010743:Phospholipids; C017475:lucifer yellow; D002784:Cholesterol