Increased levels of platelet-activating factor in blood following intestinal ischemia in the dog.
The possible role of platelet-activating factor (PAF) in superior mesenteric artery occlusion induced circulatory collapse was studied in anesthetized dogs. PAF was measured by platelet aggregation assay. Identity of PAF-like product in blood was ascertained by thin layer chromatography, high pressure liquid chromatography and alkaline treatment. Low amount of PAF was detected in the mesenteric blood under normal conditions, during reperfusion PAF levels were significantly higher. Pretreatment of the animals with BN 52021, a specific PAF receptor antagonist abolished the fall in mean arterial pressure and the rise in hematocrit due to ischemia/reperfusion. These findings suggest that PAF may play an important role in mesenteric ischemia-induced circulatory collapse.
Filep
J
J
Department of Pathophysiology, Semmelweis University Medical School, Budapest, Hungary.
Hermán
F
F
Braquet
P
P
Mózes
T
T
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004224:Diterpenes; D004285:Dogs; D046934:Ginkgolides; D007511:Ischemia / Q000097:blood; D007783:Lactones / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000097:blood*; D012769:Shock / Q000097:blood*; D013152:Splanchnic Circulation
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; C045856:ginkgolide B