Heterobifunctional cross-linking of a monoclonal antibody with 2-methyl-N1-benzenesulfonyl-N4-bromoacetylquinonediimide.
The Cyssor reagent, 2-methyl-N1-benzenesulfonyl-N4-bromoacetylquinonediimide, which will cleave a protein chain at Cys under acidic conditions, cross-linked unreduced and partially reduced antibody at pH 8.0. No cleavage of the antibody occurred suggesting that the Cyssor reagent may be useful with certain proteins as a heterobifunctional cross-linker.
Liberatore
F A
FA
Medical Products Department, E.I. du Pont de Nemours & Company, Billerica, MA 01862.
Comeau
R D
RD
Lawton
R G
RG
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents; D004121:Dimethyl Sulfoxide; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007094:Imides; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011489:Protein Denaturation
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D003432:Cross-Linking Reagents; D007094:Imides; C014090:2-methyl-N-benzenesulfonyl-N(4)-bromoacetylquinonediimide; D004121:Dimethyl Sulfoxide