The D-mannose-specific lectin from Gerardia savaglia blocks binding of human immunodeficiency virus type I to H9 cells and human lymphocytes in vitro.
The new D-mannose-specific lectin from Gerardia savaglia is shown to prevent infection of H9 cells with human immunodeficiency virus type 1 (HIV-1; strain HTLV-IIIB). At a concentration of 0.2 microM, complete protection was achieved. Even at a 50-fold higher concentration, this lectin is not toxic for the cells. Moreover, the lectin inhibits syncytium formation in the HTLV-IIIB/H9-Jurkat cell system to 100% at 0.2 microM. This effect was abolished by coaddition of D-mannose at a stoichiometric ratio of lectin to sugar of 1:500. The lectin-caused inhibition of syncytia formation was observed also in the HIV-1/human lymphocyte system. Perhaps more importantly, it is shown that the lectin reacts with the oligosaccharide side chains of the HIV-1 gp120 env molecule, which very likely can be classified to the high-mannose oligosaccharides. These data provide the basis for a rational screening for compounds interfering with gp120-CD4 interactions.
Müller
W E
WE
Institut für Physiologische Chemie, Universität, Mainz, F.R.G.
Renneisen
K
K
Kreuter
M H
MH
Schröder
H C
HC
Winkler
I
I
D000936:Antigen-Antibody Complex / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D002459:Cell Fusion / Q000187:drug effects; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects*; D015699:HIV Envelope Protein gp120; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008358:Mannose / Q000494:pharmacology; D037241:Mannose-Binding Lectins; D009844:Oligosaccharides / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000302:isolation & purification / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
D000936:Antigen-Antibody Complex; D000945:Antigens, Differentiation, T-Lymphocyte; D015699:HIV Envelope Protein gp120; D037102:Lectins; D037241:Mannose-Binding Lectins; D009844:Oligosaccharides; D015728:Receptors, HIV; D011991:Receptors, Virus; D012191:Retroviridae Proteins; D008358:Mannose