Ganglioside mapping of a human medulloblastoma xenograft.
The ganglioside patterns of medulloblastomas have never been established; in this study we report the ganglioside profile of the human medulloblastoma cell line TE-671 grown as a xenograft in nude mice. Gangliosides were isolated and structurally analyzed by fast atom bombardment mass spectometry following permethylation. Identification of individual gangliosides was also performed by immunostaining of high-performance thin-layer chromatography-separated bands. Total ganglioside levels of 0.20 mumol/g of tissue were obtained, consistent with those reported for human glioma cell lines grown as xenografts; predominant monosialogangliosides of TE-671 xenografts were II3-alpha-NeuAc-LacCer (GM3) and II3-alpha-NeuAc-GgOse3Cer (GM2) but there were also relatively large proportions of IV3-alpha-NeuAc-LcOse4Cer (3'-isoLM1), IV3-alpha-NeuAc-nLcOse4Cer (3'-LM1) and a further ganglioside of the neolacto-series with an extra lactosamine moiety. The only oligosialoganglioside detected was IV3, II3-alpha-NeuAc2-GgOse4Cer (GD1a).
Gottfries
J
J
Department of Psychiatry and Neurochemistry, Gothenburg University, St Jorgens Hospital, Sweden.
Mansson
J E
JE
Fredman
P
P
Wikstrand
C J
CJ
Friedman
H S
HS
Bigner
D D
DD
Svennerholm
L
L
D000818:Animals; D002528:Cerebellar Neoplasms / Q000378:metabolism*; D002849:Chromatography, Gas; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D008527:Medulloblastoma / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014018:Tissue Distribution; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D005732:Gangliosides