Comparative study of GABA-T from glial cells, neuronal perikarya cells and synaptosomes.
1. This paper presents a fast method of brain cell separation and a comparative study of GABA-T from different cellular compartments (glial cells, neuronal perikarya cells and synaptosomes). 2. The method of cellular separation offers the advantages of rapidity, ease and reproducibility. 3. The GABA-T from the three studied compartments had similar kinetic characteristics in respect of their Kms and Vmaxs. 4. The GABA-T needs PLP to reach its maximum activity; this dependence is related to the enzyme localization.
Bedoya
M P
MP
Facultad de Farmacia, Instituto de Bioquímica (Centro mixto C.S.I.C.-U.C.M.), Madrid, Spain.
Toledo
C
C
de Vicente
M
M
González
M P
MP
D000612:4-Aminobutyrate Transaminase / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000201:enzymology; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000201:enzymology*; D014343:Trypan Blue
D009419:Nerve Tissue Proteins; D000612:4-Aminobutyrate Transaminase; D014343:Trypan Blue