N-acetylneuraminic acid and N-glycolylneuraminic acid in glycopeptides of colonic tumor and mucosa in rats treated with carrageenan and 1,2-dimethylhydrazine.
N-linked glycopeptides were prepared from colonic tumor (adenocarcinoma) and mucosa in rats treated with carrageenan, an indigestible polysaccharide, and 1,2-dimethylhydrazine. Sialic acids, N-acetylneuraminic acid and N-glycolylneuraminic acid, obtained by acid hydrolysis of the glycopeptides were determined by HPLC. The N-acetylneuraminic acid/N-glycolylneuraminic acid ratio in colonic tumor was 25.2, while each treated mucosa had the values between 0.29 and 0.55. Thus, necessity which observes the qualitative change of sialic acid in malignant transformation was suggested.
Arakawa
S
S
Department of Hygienic Chemistry, Nagoya City University, Japan.
Wakazono
H
H
Ishihara
H
H
Tejima
S
S
D019813:1,2-Dimethylhydrazine; D000230:Adenocarcinoma / Q000032:analysis / Q000139:chemically induced; D000818:Animals; D002351:Carrageenan; D003110:Colonic Neoplasms / Q000032:analysis* / Q000139:chemically induced; D004127:Dimethylhydrazines; D006020:Glycopeptides / Q000032:analysis* / Q000302:isolation & purification; D007413:Intestinal Mucosa / Q000032:analysis / Q000187:drug effects*; D008763:Methylhydrazines; D019158:N-Acetylneuraminic Acid; D009438:Neuraminic Acids / Q000032:analysis*; D051381:Rats; D012794:Sialic Acids / Q000032:analysis*
D004127:Dimethylhydrazines; D006020:Glycopeptides; D008763:Methylhydrazines; D009438:Neuraminic Acids; D012794:Sialic Acids; C032592:N-glycolylneuraminic acid; D002351:Carrageenan; D019158:N-Acetylneuraminic Acid; D019813:1,2-Dimethylhydrazine