[Gene amplification and rearrangement of murine ribosomal RNA in virus-induced Rauscher leukemia].
Using as a hybridization probe cDNA 35s RNA from the Rauscher leukemia cells, a part of rRNA gene cluster from the gene library of mice C-1 erythroleukemia cells has been cloned. Fragment 6.7 kb recloned into pUC19 rRNA was used as a probe to analyse organization of rRNA genes of mice with RL. The observed amplification and rearrangement in genome DNA of spleen cells are determined by a new type of their rRNA genes rearrangement in the nontranscribed as well as in the transcribed part of rRNA.
Domninskiĭ
D A
DA
Pokrovskaia
E S
ES
Smirnova
I A
IA
Butenko
Z A
ZA
Grineva
N I
NI
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA; D015342:DNA Probes; D005784:Gene Amplification; D015321:Gene Rearrangement; D005796:Genes; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics; D007942:Leukemia, Experimental / Q000235:genetics*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D011061:Poly A; D012150:Polymorphism, Restriction Fragment Length; D015347:RNA Probes; D012335:RNA, Ribosomal / Q000235:genetics*; D011925:Rauscher Virus
D015342:DNA Probes; D010761:Phosphorus Radioisotopes; D015347:RNA Probes; D012335:RNA, Ribosomal; D011061:Poly A; D004247:DNA
Amplifikatsiia i perestroĭka genov ribosomnoĭ RNK myshi pri virusindutsirovannom leĭkoze Raushera.