title: A first screening for hemocompatibility of a universal support for selective and specific hemoperfusion.
abstract: A modified filmadsorber is presented, intended to be used for selective and specific hemoperfusion. It consists of a spirally wound cellulose nitrate film, onto which--after chemical activation with sodium periodate--albumin is bound, stabilized, sterilized and activated with glutaraldehyde. Various bioactive ligands containing amino groups can be coupled to this support. A subsequent treatment with dimethylamino borane stabilizes the bonds between cellulose nitrate, albumin, glutaraldehyde and ligand. Columns in which a second layer of albumin is bound to the support as a model for a bioactive ligand were first screened for hemocompatibility using rabbits. Leukocyte, thrombocyte and hematocrit behaviour during hemoperfusion showed that hemocompatibility of the support was good.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, Eindhoven University of Technology, the Netherlands.
authors.forename: M L
authors.initials: ML
authors.lastname: Verhoeven
authors.forename: M
authors.initials: M
authors.lastname: Böttcher
authors.forename: F M
authors.initials: FM
authors.lastname: Everaerts
authors.forename: L A
authors.initials: LA
authors.lastname: Sluyterman
authors.forename: B
authors.initials: B
authors.lastname: Ernst
authors.forename: H
authors.initials: H
authors.lastname: Klinkmann
mesh_terms: D000327:Adsorption; D000418:Albumins; D000596:Amino Acids; D000818:Animals; D002482:Cellulose; D006464:Hemoperfusion / Q000295:instrumentation*; D007958:Leukocyte Count; D008024:Ligands; D010976:Platelet Count; D011817:Rabbits
chemical_list: D000418:Albumins; D000596:Amino Acids; D008024:Ligands; D002482:Cellulose