Exocytotic release of neurotransmitter substances from nerve endings in the taste buds of rat circumvallate papillae.
Exocytotic release of neurotransmitters from nerve endings was demonstrated ultrastructurally in the taste buds of rat circumvallate papillae by stimulation of high K+ and Ca2+ Ringer perfusion and application of tannic acid-Ringer incubation (TARI) method. Omega-shaped images of large cored vesicles and small clear vesicles, indicating exocytotic release of their contents, were found only in the non-synaptic sites. Occasionally exocytosis occurred at sites facing other nerve fibers. Many coated pits were also seen, which presumably represent membrane retrieval at a later stage of exocytosis. It is likely that the taste buds receive more than one type of innervation.
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Department of Applied Biology, Kyoto Institute of Technology, Japan.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003034:Coated Pits, Cell-Membrane / Q000502:physiology / Q000648:ultrastructure; D005089:Exocytosis / Q000187:drug effects; D047348:Hydrolyzable Tannins; D007552:Isotonic Solutions; D008297:Male; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000378:metabolism* / Q000648:ultrastructure; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D000077331:Ringer's Solution; D013569:Synapses / Q000648:ultrastructure; D013650:Taste Buds / Q000378:metabolism* / Q000648:ultrastructure
D047348:Hydrolyzable Tannins; D007552:Isotonic Solutions; D018377:Neurotransmitter Agents; D000077331:Ringer's Solution; D011188:Potassium; D002118:Calcium