Molecular sorting in the secretory pathway.
Proteins can be secreted from animal cells by either a constitutive or a regulated pathway; those destined for regulated secretion are actively sorted into dense-core secretory granules. Although sorting is generally assumed to be accomplished by specific carriers, the nature of these carriers remains elusive. In this study, peptide hormones were used as affinity ligands to purify a set of 25-kilodalton proteins from canine pancreatic tissue. Their ligand specificities and patterns of expression have the characteristics of sorting carriers.
Chung
K N
KN
Department of Physiology Anatomy, University of California, Berkeley 94720.
Walter
P
P
Aponte
G W
GW
Moore
H P
HP
D000818:Animals; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods; D004285:Dogs; D006056:Golgi Apparatus / Q000378:metabolism*; D007106:Immune Sera; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000378:metabolism*; D008970:Molecular Weight; D010179:Pancreas / Q000378:metabolism*; D011388:Prolactin
D000936:Antigen-Antibody Complex; D002352:Carrier Proteins; D007106:Immune Sera; D011388:Prolactin