Liquid-chromatographic determination of acetylated hemoglobin.
In this liquid-chromatographic assay for acetylated hemoglobin in human blood, glycated hemoglobins in hemolysates are first removed by affinity chromatography on boronate-agarose columns. Acetylated hemoglobin in the nonretained fraction is determined by cation-exchange chromatography. The absorbance of the effluent is monitored at 415 nm. The mean within-assay CV was 8.5%, the between-assay CV 17%. The mean proportion of acetylated hemoglobin in 20 pregnant, nondiabetic women was 1.9%, and in 17 alcoholics it was 2.7%. Rapid and reproducible, this method is suitable for use in routine determination of acetylated hemoglobin.
Turpeinen
U
U
Department I of Obstetrics & Gynecology, Helsinki University Central Hospital, Finland.
Stenman
U H
UH
Roine
R
R
D000107:Acetylation; D000437:Alcoholism / Q000097:blood; D002021:Buffers; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D011247:Pregnancy; D011786:Quality Control; D012016:Reference Values
D002021:Buffers; D006454:Hemoglobins; C058244:acetylated hemoglobin