Murine leukemic cells inhibit IL 2 production by susceptible syngeneic splenocytes.
BALB/c mice fail to mount significant cell-mediated immunity against the syngeneic virally induced leukemia MCDV-12, and die approximately 10 days after tumor inoculation. Our studies in vitro demonstrated that BALB/c splenocyte and irradiated MCDV-12 cell co-culture led to reduced alloreactivity, including depressed interleukin 2 (IL 2) production. Tumor-induced immune suppression was genetically restricted, antigen nonspecific, and alleviated in part by exogenous IL 2 administration in vitro. Furthermore, mitogen stimulation of IL 2 production, not requiring self or alloantigen recognition, was unaffected by MCDV-12 exposure. These results indicate that tumor cells may escape from immunosurveillance by reducing IL 2 production in the immediate tumor vicinity, and suggest a potential role for major histocompatibility complex antigens in the immunosuppression mechanism.
Jenski
L J
LJ
Ledford
T
T
D000818:Animals; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D007108:Immune Tolerance; D007113:Immunity, Innate; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000502:physiology; D007938:Leukemia / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D051381:Rats; D011925:Rauscher Virus / Q000276:immunology; D013154:Spleen / Q000166:cytology*
D007376:Interleukin-2; D003208:Concanavalin A