The effects of serotonin agonists and antagonists on the response properties of complex ganglion cells in the rabbit's retina.
Selective agonists and antagonists were employed to determine the role of indoleaminergic amacrine cells in the generation of the light-evoked responses and spontaneous activity of direction and orientation selective cells. Perfusion with 5-HT2 antagonists reduced the spontaneous activity and both the leading and trailing edge responses of ON/OFF direction selective cells. 5-HT1a agonists had a similar effect on this class of cell, namely, a reduction of light-evoked and spontaneous activity. Results from ON-center and OFF-center orientation selective cells were consistent with those obtained from direction selective cells in that no disruption of direction or orientation selectivity was observed during perfusion of these drugs. These data suggest that the indoleaminergic cells are not directly involved in the generation of the trigger features of complex ganglion cells, but may be facilitating synaptic transmission in the inner retina. This function is discussed relative to the connectivity of the rod bipolar cells and the putative indoleaminergic amacrine cells. The similarity of the effects of 5-HT1a agonists and 5-HT2 antagonists supports the hypothesis, developed during our prior studies of brisk ganglion cells, that these two receptor classes mediate antagonistic processes in the target neurons.
Brunken
W J
WJ
Washington University School of Medicine, Department of Cell Biology and Physiology, St. Louis.
Daw
N W
NW
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000818:Animals; D015259:Dopamine Agents; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008732:Methoxydimethyltryptamines; D011817:Rabbits; D011985:Receptors, Serotonin / Q000187:drug effects; D012160:Retina / Q000166:cytology / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000502:physiology*; D012702:Serotonin Antagonists; D013569:Synapses / Q000502:physiology; D013764:Tetrahydronaphthalenes; D014795:Visual Pathways / Q000187:drug effects / Q000502:physiology
D015259:Dopamine Agents; D008732:Methoxydimethyltryptamines; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D013764:Tetrahydronaphthalenes; D012701:Serotonin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin