Chromosome aberrations induced by aflatoxin B1 in rat bone marrow cells in vivo and their suppression by green tea.
Aflatoxin B1 (AFB1)-induced chromosome aberrations (CA) in rat bone marrow cells consisted mainly of gaps and breaks. Cells with exchanges and multiple CA were observed infrequently. The incidence of aberrant cells and the number of aberrations per cell were at their maximum levels 18 h after the AFB1 injection. They were dependent on the administered dose of AFB1. Rats given the hot water extract from green tea (GTE) 24 h before they were injected with AFB1 displayed considerably suppressed AFB1-induced CA in their bone marrow cells. Rats administered GTE 2 h before or after the AFB1 injection showed no suppressive effect. The suppressive effect of GTE on AFB1-induced CA paralleled the dose of GTE when given in the range between 0.1 and 2 g/kg body weight; higher doses produced no additional suppression. On the other hand, rats given the hot water extract from black tea or coffee 24 or 2 h before the AFB1 injection showed no suppressive effect. The administration of caffeine 24 h before the AFB1 injection suppressed AFB1-induced CA as well as the administration of caffeine 2 h before the AFB1 injection. However, the suppression rate with 2 h was larger than with 24 h. The suppression by ellagic acid was found only when it was given 2 h before the AFB1 injection. The administration of ascorbic acid or tannic acid did not significantly suppress AFB1-induced CA. The tannin mixture extracted from green tea (GTTM) showed a similar tendency to GTE, that is, the administration of GTTM 24 h before the AFB1 injection potently suppressed AFB1-induced CA, while the administration of GTTM 2 h before the AFB1 injection did not suppress them significantly. The suppressive effect of GTTM on AFB1-induced CA paralleled the dose of GTTM when given in the range of 75-450 mg/kg body weight.
Ito
Y
Y
Public Health Research Institute, Kobe City, Japan.
Ohnishi
S
S
Fujie
K
K
D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000627:therapeutic use; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology; D002110:Caffeine / Q000008:administration & dosage / Q000627:therapeutic use; D002869:Chromosome Aberrations; D007274:Injections, Intraperitoneal; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013634:Tannins / Q000008:administration & dosage / Q000627:therapeutic use; D013662:Tea
D000348:Aflatoxins; D013634:Tannins; D013662:Tea; D002110:Caffeine; D001205:Ascorbic Acid