title: Chlorella viruses contain linear nonpermuted double-stranded DNA genomes with covalently closed hairpin ends.
abstract: Pulsed field electrophoresis established that Chlorella viruses contain linear, nonpermuted, 330- to 380-kb dsDNA genomes. Terminal DNA restriction fragments of one virus, PBCV-1, were identified by Bal31 exonuclease digestion; the termini probably contain covalently closed hairpin ends. The end fragments cross-hybridize indicating terminal repetition; the region of repetition extends no more than 2.5 kb from the ends.
authors.affiliation: Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
authors.forename: J
authors.initials: J
authors.lastname: Rohozinski
authors.forename: L E
authors.initials: LE
authors.lastname: Girton
authors.forename: J L
authors.initials: JL
authors.lastname: Van Etten
mesh_terms: D002708:Chlorella; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D005814:Genes, Viral; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D014780:Viruses / Q000235:genetics*
chemical_list: D004279:DNA, Viral