High resolution chromosomes from first trimester trophoblast cultures.
A simple method for obtaining prometaphase chromosomes from cultured first trimester cells involves the addition of BrdU and FdU 11 h before harvest and ethidium bromide 1.5 h before harvest. High resolution R-banding is obtained by acridine orange staining.
Søndergaard
F
F
Kristensen
M
M
Tommerup
N
N
D001973:Bromodeoxyuridine; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D004996:Ethidium; D005260:Female; D005467:Floxuridine; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011296:Prenatal Diagnosis / Q000379:methods*; D014327:Trophoblasts / Q000648:ultrastructure*
D005467:Floxuridine; D004996:Ethidium; D001973:Bromodeoxyuridine