title: DNA restriction fragment length polymorphisms at either end of the c-raf-1 locus at 3p25.
abstract: We report the identification and characterization of two restriction fragment polymorphisms (RFLPs) associated with the human c-raf-1 protooncogene located on chromosome 3 (3p25). EcoRI digestion produces an RFLP derived from the 5'flanking sequences with a heterozygosity of 28%, and TaqI produces an RFLP representing the 3' flanking sequences with a heterozygosity of 25%. The finding of RFLPs associated with a potential oncogene may provide a method of analysis for determining genetic susceptibilities to certain cancers.
authors.affiliation: Department of Medicine, SUNY-Health Science Center, Syracuse 13210.
authors.forename: S L
authors.initials: SL
authors.lastname: Graziano
authors.forename: G E
authors.initials: GE
authors.lastname: Mark
authors.forename: C
authors.initials: C
authors.lastname: Murray
authors.forename: D L
authors.initials: DL
authors.lastname: Mann
authors.forename: G D
authors.initials: GD
authors.lastname: Ehrlich
authors.forename: B J
authors.initials: BJ
authors.lastname: Poiesz
authors.forename: A
authors.initials: A
authors.lastname: Weston
mesh_terms: D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D006801:Humans; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf
chemical_list: D011518:Proto-Oncogene Proteins; D019908:Proto-Oncogene Proteins c-raf