Superhelical DNA-dependent ATPase from calf thymus.
Two physically and catalytically distinct DNA-dependent ATPases were isolated from a purified preparation of calf thymus poly(ADP-ribose) polymerase. A unique feature of these two ATPases was the high stimulation by supercoiled DNA. Other nucleic acids (including denatured DNA and ribosomal RNA) and certain polynucleotides differentially stimulated the two enzymes. We have not detected any other DNA-related activity associated with these ATPases.
Zahradka
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P
Ebisuzaki
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K
D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000302:isolation & purification / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002412:Cations; D002417:Cattle; D002845:Chromatography; D004247:DNA / Q000494:pharmacology; D004278:DNA, Superhelical / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis; D012335:RNA, Ribosomal / Q000494:pharmacology; D013950:Thymus Gland / Q000201:enzymology*
D002412:Cations; D004278:DNA, Superhelical; D012335:RNA, Ribosomal; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D000251:Adenosine Triphosphatases