title: Molecular cloning of the type 5, iron-containing, tartrate-resistant acid phosphatase from human placenta.
abstract: The type 5, iron-containing, tartrate-resistant acid phosphatase (TR-AP) constitutes a relatively minor intracellular isozyme of acid phosphatase in the human that is immunologically related to uteroferrin, a secreted progesterone-induced protein of the porcine uterus. Here, the purification of small amounts of TR-AP from human placenta is described. When a placental lambda gt11 cDNA library was screened with two short 32P-labeled cDNA clones from within the coding region of uteroferrin, a 1412-base pair cDNA was identified that encodes the entire human TR-AP isozyme. This cDNA contains an open reading frame of 969 base pairs, corresponding to a protein of 323 amino acids. A putative signal sequence of 19 amino acids and two potential glycosylation sites are present. The deduced amino acid sequence of the human TR-AP is 85% identical to that of porcine uteroferrin (whose sequence is also reported here in complete form for the first time) and 82% identical to the corresponding regions of a partial amino acid sequence of a bovine spleen phosphoprotein phosphatase. Northern blotting techniques employing a labeled TR-AP cDNA probe revealed the presence of a 1.5-kilobase transcript in white cells from a patient with hairy cell leukemia, in human K562 erythroleukemic cells, and in Epstein-Barr virus-transformed human B-cells, but not in a human T-cell line. Culture of K562 cells in presence of 10(-8) M phorbol 12-myristate 13-acetate ester for 48-72 h enhanced TR-AP activity per cell about 30-fold and led to a corresponding increase in TR-AP mRNA levels.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Florida, Gainesville 32610.
authors.forename: C M
authors.initials: CM
authors.lastname: Ketcham
authors.forename: R M
authors.initials: RM
authors.lastname: Roberts
authors.forename: R C
authors.initials: RC
authors.lastname: Simmen
authors.forename: H S
authors.initials: HS
authors.lastname: Nick
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D007501:Iron; D007527:Isoenzymes / Q000235:genetics*; D008667:Metalloproteins / Q000235:genetics; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D000071681:Tartrate-Resistant Acid Phosphatase; D013644:Tartrates; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D007527:Isoenzymes; D008667:Metalloproteins; D012333:RNA, Messenger; D013644:Tartrates; D007501:Iron; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D013755:Tetradecanoylphorbol Acetate