Automated determination of urinary Na+, K+, chloride, inorganic phosphate, urea, and creatinine without sample dilution, with the "RA-XT".
We describe how concentrations of chloride, urea, inorganic phosphate, and creatinine in urine can be measured directly, without manual sample dilution, in a discrete analyzer (the Technicon "RA-XT"). These methods were accurate for concentrations of chloride up to 280 mmol/L, urea up to 500 mmol/L, inorganic phosphate up to 50 mmol/L, and creatinine up to 30 mmol/L. CVs are less than 3% nd results correlate well with those obtained by continuous-flow analysis (SMA-II). All these reagents are stable at room temperature for three weeks. Analyses are easy to perform and infrequent calibration is required.
Fesus
P D
PD
Laboratoire Biochimie, Hôpital Trousseau, Paris, France.
Pressac
M
M
Braconnier
F
F
Aymard
P
P
D001322:Autoanalysis / Q000295:instrumentation*; D002712:Chlorides / Q000652:urine; D003404:Creatinine / Q000652:urine*; D004573:Electrolytes / Q000652:urine*; D006801:Humans; D010710:Phosphates / Q000652:urine; D011188:Potassium / Q000652:urine; D011786:Quality Control; D012016:Reference Values; D012044:Regression Analysis; D012964:Sodium / Q000652:urine; D014508:Urea / Q000652:urine*
D002712:Chlorides; D004573:Electrolytes; D010710:Phosphates; D014508:Urea; D012964:Sodium; D003404:Creatinine; D011188:Potassium