title: Influence of genetically inherited complement deficiencies on humoral immune response in guinea pigs.
abstract: To assess the role of complement in the induction of the humoral immune response, we studied the antibody response of guinea pigs genetically deficient in the second component of the classical complement pathway (C2D-GP) to bacteriophage phi X 174--a T cell-dependent antigen--in comparison with normal guinea pigs and C4D-GP, for which a disturbance in induction of antibody response has been described. We were able to establish a clear dose-response relationship: with low doses of antigen (1 X 10(9) PFU/kg), the antibody response of both complement-deficient strains was grossly impaired as compared with normal guinea pigs. After primary immunization, the peak antibody titer was diminished (1 log10) and declined rapidly; after secondary immunization, the diminution became even more distinct. Both complement-deficient strains had unusual secondary antibody responses almost identical to their primary ones, and amplification of antibody titer, as well as regular isotype switch from IgM to IgG, was absent. By increasing the antigen dose (2 X 10(9) PFU/kg), the antibody responses of the complement-deficient guinea pigs tend to normalize, and when high doses of antigen (1 X 10(10) PFU/kg) were used, the behavior of the complement-deficient animals was nearly indistinguishable from that of normal animals. Partial restoration of the immune response was seen when substituting the genetic complement deficiency by giving serum as source of the missing complement component. The important contribution of the C2 deficiency is given by the now compelling evidence that it is not the missing individual component itself, but rather the common block in sequential activation of C3 via the classical pathway in both complement deficiencies, that is responsible for the impaired humoral immune response, especially at low antigen doses. We therefore postulate that an intact classical pathway contributes to reaching a normal humoral immune response.
authors.forename: E C
authors.initials: EC
authors.lastname: Böttger
authors.forename: T
authors.initials: T
authors.lastname: Hoffmann
authors.forename: U
authors.initials: U
authors.lastname: Hadding
authors.forename: D
authors.initials: D
authors.lastname: Bitter-Suermann
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000008:administration & dosage / Q000276:immunology; D010584:Bacteriophage phi X 174 / Q000276:immunology*; D003175:Complement C2 / Q000008:administration & dosage / Q000172:deficiency / Q000235:genetics; D003181:Complement C4 / Q000008:administration & dosage / Q000172:deficiency / Q000235:genetics; D003165:Complement System Proteins / Q000172:deficiency* / Q000235:genetics; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006168:Guinea Pigs; D006579:Heterozygote; D008297:Male; D009500:Neutralization Tests
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D003175:Complement C2; D003181:Complement C4; D003165:Complement System Proteins