Colorimetric method for assay of serum gamma-glutamyltransferase activity with some L-gamma-glutamyl-carboxyanilides.
A simple method for the assay of serum gamma-glutamyltransferase activity with soluble L-gamma-glutamyl-carboxyanilides is presented. It is based on transformation of aminobenzoic acid liberated by the enzyme into colour complex with 4-dimethyl-aminobenzaldehyde or with 4-dimethylaminocinnamaldehyde in acid medium. Among three carboxyanilides studied the highest enzyme activity was noted with L-gamma-glutamyl-3-carboxyanilide and no activity with L-gamma-glutamyl-2-carboxyanilide. A good correlation was demonstrated for serum gamma-glutamyltransferase activity determined by the new colorimetric method and by the standard one with L-gamma-glutamyl-3-carboxy-4-nitroanilide.
Szewczuk
A
A
Biochemical Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocøaw.
Kuropatwa
M
M
Lang
D
D
D000813:Anilides; D000818:Animals; D001547:Benzaldehydes; D002417:Cattle; D002934:Cinnamates; D003124:Colorimetry; D007700:Kinetics; D005723:gamma-Glutamyltransferase / Q000097:blood*
D000813:Anilides; D001547:Benzaldehydes; D002934:Cinnamates; C018523:4-dimethylaminocinnamaldehyde; D005723:gamma-Glutamyltransferase; C510241:p-dimethylaminobenzaldehyde