Analysis of the HLA-A10-crossreacting group of antigens by one-dimensional isoelectric focusing.
The antigens belonging to the HLA-A10 group, HLA-A25, -A26, -Aw34, and -Aw66, have been characterized serologically during the International Histocompatibility Workshops. However, it remains difficult to discriminate between the HLA-A26 antigen on the one hand and the HLA-Aw34 and -Aw66 antigens on the other on the basis of serology. In this paper, we compare the serologically defined antigens with the data obtained by one-dimensional isoelectric focusing. The results indicate that the serologically well-defined HLA-A25 antigen cannot be discriminated from the HLA-A26 antigen by one-dimensional isoelectric focusing. In contrast, this technique can indeed be used to discriminate between HLA-A26, -Aw34 and -Aw66 antigens. In addition, the biochemical analysis suggests further heterogeneity of the HLA-Aw34 antigen. This antigen can be subdivided into three variants.
van der Horst
A R
AR
Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Lardy
N M
NM
de Waal
L P
LP
D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D005260:Female; D015234:HLA-A Antigens / Q000276:immunology*; D006801:Humans; D007525:Isoelectric Focusing; D011247:Pregnancy; D012699:Serology
D000911:Antibodies, Monoclonal; D015234:HLA-A Antigens; C039682:HLA-A10 antigen