Chromosomal mapping in Erwinia carotovora subsp. carotovora with the IncP plasmid R68::Mu.
Conjugational gene transfer was established in Erwinia carotovora subsp. carotovora SCRI193 by using plasmid R68::Mu c+ to mobilize the chromosome into multiply mutant recipients. It was observed that although the plasmid alone mobilized markers randomly at a frequency of ca. 10(-5) chromosomal recombinants per donor, the presence of a Mu prophage on the chromosome of the donor increased the frequency of mobilization of markers adjacent to the prophage by up to 10-fold. Using this system it was possible to order 17 chromosomal mutations. The behavior of Mu in E. carotovora subsp. carotovora was also studied.
Forbes
K J
KJ
Pérombelon
M C
MC
D010583:Bacteriophage mu / Q000235:genetics; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004885:Erwinia / Q000235:genetics*; D009154:Mutation; D010957:Plasmids