Cytology of intraocular lens surface: a transmission electron-microscopic study. Cytology of intraocular lens surface.
We developed a new method for specimen preparation for transmission electronmicroscopic study of cellular responses on intraocular lenses. This procedure omits propylene oxide treatment of the materials to be studied, which is normally a step in the routine preparation process for transmission electron microscopy. It was previously found that propylene oxide treatment dissolves intraocular lenses. Using our procedure, two types of cells were identified on intraocular lenses removed from monkey eyes 7 days after implantation. These cells had the typical structural features of multinucleated giant cells and of macrophages. It thus appears that this procedure will permit study of the cytology of intraocular lens surfaces.
Ishibashi
T
T
Department of Ophthalmology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Sugai
S
S
Kubota
T
T
Ohnishi
Y
Y
Inomata
H
H
D000818:Animals; D005123:Eye / Q000166:cytology*; D007910:Lenses, Intraocular; D008251:Macaca; D008566:Membranes / Q000166:cytology / Q000648:ultrastructure; D008854:Microscopy, Electron; D013508:Ophthalmologic Surgical Procedures; D013048:Specimen Handling