Simultaneous measurement by flow cytometry of phagocytosis and hydrogen peroxide production of neutrophils in whole blood.
Bacterial ingestion and hydrogen peroxide production by polymorphonuclear leukocytes (PMN) were simultaneously measured using flow cytometry and 2',7'-dichlorofluorescein diacetate and fluorescent Staphylococcus aureus. Only 100 microliters of a whole blood specimen were required for these determinations, and the results were found to be independent of the absolute numbers of PMN, making the purification and adjustment of PMN numbers unnecessary. A positive correlation between phagocytosis and hydrogen peroxide production by individual PMN was demonstrated in healthy adults.
Hasui
M
M
Department of Pediatrics, Kansai Medical University, Osaka, Japan.
Hirabayashi
Y
Y
Kobayashi
Y
Y
D000328:Adult; D004906:Erythrocyte Count; D005434:Flow Cytometry / Q000379:methods; D005452:Fluoresceins; D006801:Humans; D006861:Hydrogen Peroxide / Q000096:biosynthesis* / Q000097:blood; D007958:Leukocyte Count; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D010587:Phagocytosis; D011419:Propidium
D005452:Fluoresceins; D011419:Propidium; C037631:2',7'-dichlorofluorescein; D006861:Hydrogen Peroxide