Basolateral amino acid and glucose transport by the intestine of the teleost, Anguilla anguilla.
1. D-glucose transport into BLMV was osmotically reactive, sodium independent, and inhibited by phloretin but not by phloridzin. 2. The survey of 6 L-amino acids identified three groups with respect to transfer across the basolateral cell border. Transport of proline and glutamate occurred by Na-dependent carriers and by apparent simple diffusion. Alanine, lysine and phenylalanine were transported by Na-independent carriers and apparent simple diffusion. Glycine transport was stimulated above apparent simple diffusion only by a simultaneous inwardly-directed Na gradient and outwardly-directed K gradient. 3. Only proline and glutamate demonstrated the ability to depolarize the membrane potential, consistent with Na-dependent rheogenic transport.
Reshkin
S J
SJ
Department of Zoology, University of Hawaii, Honolulu 96822.
Vilella
S
S
Cassano
G
G
Ahearn
G A
GA
Storelli
C
C
D000596:Amino Acids / Q000378:metabolism*; D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D002412:Cations; D002462:Cell Membrane / Q000201:enzymology; D005947:Glucose / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D007700:Kinetics; D008024:Ligands; D008564:Membrane Potentials; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D009997:Osmotic Pressure; D010693:Phloretin / Q000494:pharmacology
D000596:Amino Acids; D002412:Cations; D008024:Ligands; D005947:Glucose; D010693:Phloretin