Novel O-linked carbohydrate chains in the cellulase complex (cellulosome) of Clostridium thermocellum. 3-O-Methyl-N-acetylglucosamine as a constituent of a glycoprotein.
Alkaline borohydride treatment of the cellulosome of Clostridium thermocellum yielded two major oligosaccharide-alditols, namely D-Galp-beta(1----4)-D-GalOH and (formula; see text) The compounds, isolated via gel permeation chromatography and high performance liquid chromatography, were analyzed by monosaccharide analysis, methylation analysis, gas-liquid chromatography/mass spectrometry, fast atom bombardment/mass spectrometry, and one- and two-dimensional 500-MHz (COSY, HOHAHA, ROESY) 1H NMR spectroscopy. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with blotting technology indicated that the tetrasaccharide is mainly associated with one of the cellulosome subunits.
Gerwig
G J
GJ
Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
de Waard
P
P
Kamerling
J P
JP
Vliegenthart
J F
JF
Morgenstern
E
E
Lamed
R
R
Bayer
E A
EA
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002480:Cellulase; D002851:Chromatography, High Pressure Liquid; D003013:Clostridium / Q000201:enzymology*; D005944:Glucosamine / Q000031:analogs & derivatives*; D006023:Glycoproteins; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009844:Oligosaccharides / Q000302:isolation & purification*
D006023:Glycoproteins; D009097:Multienzyme Complexes; D009844:Oligosaccharides; C014088:methyl-2-acetamido-2-deoxy-D-glucopyranoside; D002480:Cellulase; D005944:Glucosamine; D000117:Acetylglucosamine