Antibodies raised against synthetic peptides from the Arg-Gly-Asp-containing region of the Leishmania surface protein gp63 cross-react with human C3 and interfere with gp63-mediated binding to macrophages.
The Leishmania surface glycoprotein gp63 binds to complement receptor type 3 on the macrophage surface. Antibody raised against a synthetic peptide containing the Arg-Gly-Asp region of the amino acid sequence of gp63 recognizes both gp63 and the alpha-chain of human C3. Monovalent Fab fragments from this antibody block gp63-mediated binding to macrophages.
Russell
D G
DG
Department of Pathology, New York University Medical Center 10016.
Talamas-Rohana
P
P
Zelechowski
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003176:Complement C3 / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D007894:Leishmania mexicana / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*
D000913:Antibodies, Protozoan; D003176:Complement C3; D008562:Membrane Glycoproteins; D010446:Peptide Fragments