title: Cyclin mRNA and protein expression in recombinant interleukin 2-stimulated cloned murine T lymphocytes.
abstract: Expression of cyclin, a non-histone nuclear protein, during recombinant interleukin 2 (rIL2)-driven cell-cycle progression of cloned T lymphocytes has been assessed. We found that expression of cyclin protein, as detected by immunofluorescence, is tightly associated with proliferation, and not merely S-phase, of L2 cells stimulated with rIL2. Cyclin immunofluorescence was detected in all cell-cycle phases (G1/S/G2/M, as detected by flow cytometry) of proliferating L2 cells. Accumulation of cyclin mRNA levels was induced as early as 1 h after stimulation, was maximal at 25-49 h, and remained elevated throughout stimulation, as detected by Northern blot analysis. A cDNA-encoding murine cyclin was cloned from a cDNA library prepared from IL2-stimulated cloned T cells. The sequence of the 5' end of the murine cyclin cDNA was determined and found to be 88% and 82% similar to the sequences of cDNA clones encoding rat and human cyclin, respectively. The present studies demonstrate that cyclin protein and mRNA accumulation are highly regulated during IL2-induced proliferation of a cloned T cell. These data provide a framework for addressing the molecular mechanisms regulating cyclin gene expression during cellular proliferation.
authors.affiliation: Department of Pathology, University of Pennsylvania, Philadelphia 19104-6082.
authors.forename: P M
authors.initials: PM
authors.lastname: Shipman
authors.forename: D E
authors.initials: DE
authors.lastname: Sabath
authors.forename: A H
authors.initials: AH
authors.lastname: Fischer
authors.forename: P G
authors.initials: PG
authors.lastname: Comber
authors.forename: K
authors.initials: K
authors.lastname: Sullivan
authors.forename: E M
authors.initials: EM
authors.lastname: Tan
authors.forename: M B
authors.initials: MB
authors.lastname: Prystowsky
mesh_terms: D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D007376:Interleukin-2 / Q000494:pharmacology; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis*; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011994:Recombinant Proteins; D013045:Species Specificity; D013601:T-Lymphocytes
chemical_list: D007376:Interleukin-2; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D011994:Recombinant Proteins; D004247:DNA