Influence of complement C5 and V beta T cell receptor mutations on susceptibility to collagen-induced arthritis in mice.
SWR/J mice are resistant to collagen-induced arthritis (CIA) despite having a susceptible H-2q haplotype. We have earlier demonstrated the possible role of the V beta TCR mutation of SWR in the resistance to CIA. To investigate the influence of the C5 deficiency of SWR in this resistance, crosses were made between SWR and A/J (C5 deficient, TCRwild, H-2a), and between SWR and C3H.A (C5 sufficient, TCRwild, H-2a). Upon immunization with bovine type II collagen in adjuvant, there was a similar incidence and severity of arthritis in H-2q-bearing mice in the back-crosses A x (SWR x A) ad C3H.A x (SWR x C3H.A). The absence of hemolytic complement was confirmed in the arthritic A x (SWR x A) back-cross mice by standard SRBC hemolytic assays. In addition C57L (H-2b) mice, which were C5 sufficient but had a V beta TCR deletion mutation similar to SWR, could not complement for CIA susceptibility in H-2q-bearing C57L x (SWR x C57L) back-crosses and in (C57L x SWR)F2 hybrids. These studies show that complement C5 does not play a significant role in CIA susceptibility, and further implicate the V beta TCR mutation in the resistance to CIA in SWR mice.
Banerjee
S
S
Department of Immunology, Mayo Clinic, Rochester, MN 55905.
Anderson
G D
GD
Luthra
H S
HS
David
C S
CS
D000818:Animals; D001168:Arthritis / Q000235:genetics*; D001169:Arthritis, Experimental / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D001323:Autoantibodies / Q000096:biosynthesis; D003094:Collagen / Q000276:immunology; D003182:Complement C5 / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D003433:Crosses, Genetic; D006461:Hemolysis; D007113:Immunity, Innate; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009154:Mutation; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000502:physiology
D001323:Autoantibodies; D003182:Complement C5; D011948:Receptors, Antigen, T-Cell; D003094:Collagen