title: Caldesmon is present in human and pig erythrocytes.
abstract: Caldesmon, a major actin- and calcium-dependent calmodulin-binding protein, is now considered as an essential inhibitory factor of the actomyosin machinery in smooth muscle cells as well as in non-muscle cells. Since its structure seems to vary with the cell in a same species and because platelet and erythrocyte have a common embryonic origin, we have used the affinity purified antibody raised against the platelet caldesmon to determine whether this protein is present in erythrocyte. Using the immunoblotting technique, we show here that, in whole erythrocytes, only a single polypeptide crossreacts with this polyclonal antibody. A 71-72 kDa Mr has been calculated from SDS-PAGE. It is therefore different from those of the gizzard (Mr 145-150 kDa) or the platelet (Mr 80 kDa) proteins. Furthermore, we also give evidence that it is not adducin since this newly discovered calcium-dependent calmodulin-binding protein of erythrocyte, does not crossreact with the polyclonal affinity purified antibody raised against platelet caldesmon.
authors.affiliation: Institut de Biologie Physico-Chimique, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: der Terrossian
authors.forename: C
authors.initials: C
authors.lastname: Deprette
authors.forename: R
authors.initials: R
authors.lastname: Cassoly
mesh_terms: D000818:Animals; D000906:Antibodies; D001792:Blood Platelets / Q000032:analysis; D002148:Calmodulin-Binding Proteins / Q000276:immunology / Q000302:isolation & purification*; D003429:Cross Reactions; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D011817:Rabbits; D013552:Swine; D014335:Tropomyosin / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D002148:Calmodulin-Binding Proteins; D014335:Tropomyosin