XANES spectroscopy sensitivity to small electronic changes. Case of carp azidomethemoglobin.
Spin states equilibrium of hemoglobin-iron varies with external conditions: pH, allosteric effectors, temperature. The small electronic reorganization of the iron caused by the spin state changes has been detected by X-ray absorption near edge structure (XANES) spectroscopy at room temperature. The iron K-edge region which is sensitive to spin state is located in 7110-7130 eV. Here are presented the 100% high spin and 100% low spin XANES spectra of carp azido ferric hemoglobin.
Pin
S
S
Laboratoire de Biologie Physico-Chimique, Université Paris VII, France.
Le Tilly
V
V
Alpert
B
B
Cortes
R
R
D000818:Animals; D002347:Carps; D008706:Methemoglobin / Q000031:analogs & derivatives*; D013052:Spectrometry, X-Ray Emission
C029387:methemoglobin azide; D008706:Methemoglobin