Abortive replication of choleraphage phi 149 in Vibrio cholerae biotype el tor.
Choleraphage phi 149 adsorbed irreversibly to Vibrio cholerae biotype el tor cells, and 50% of the injected phage DNA bound to the cell membrane. Although no infectious centers were produced at any time during infection, the host macromolecular syntheses were shut off and the host DNA underwent chloramphenicol-inhibitable degradation. Synthesis of monomeric phage DNA continued similar to that observed in the permissive host. However, the concatemeric DNA intermediates produced were unstable and could not be chased to mature phage DNA. Pulse-labeling of UV-irradiated infected cells at different times during infection allowed identification of phage-specific proteins made in this nonpermissive host. Although most of the early proteins were made, only some of the late proteins were transiently synthesized.
Chowdhury
R
R
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
Biswas
S K
SK
Das
J
J
D001435:Bacteriophages / Q000502:physiology*; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism; D014734:Vibrio cholerae; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins