A rabbit lens epithelial cell line supports expression of an exogenous crystallin gene characteristic of lens fiber cell differentiation.
Cell lines derived from the lens generally fail to maintain synthesis of crystallins in long-term culture. Here we demonstrate that the N/N1003A line of undifferentiated lens epithelial cells, derived from a newborn rabbit, does not produce detectable levels of alpha-, beta- or gamma-crystallin transcripts, yet is capable of supporting the transient expression of the mouse gamma 2-crystallin promoter, a promoter which is active only in terminally differentiated lens fiber cells in vivo. Analysis of a set of deletion constructs suggested that sequences required for activity of the mouse promoter in N/N1003A cells are similar, but not identical, to those previously shown to be essential in primary chick embryo lens explants. Therefore, these results suggest that different transcriptional factors may be capable of supporting lens-specific activity of the mouse gamma 2 promoter. In addition, this cell line, N/N1003A, should be useful for investigations on the elements regulating gamma-crystallin gene expression.
Meakin
S O
SO
Division of Cancer and Cell Biology, Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada.
Reddan
J R
JR
Tsui
L C
LC
Breitman
M L
ML
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002460:Cell Line; D002642:Chick Embryo; D003459:Crystallins / Q000096:biosynthesis*; D004848:Epithelium / Q000378:metabolism; D005796:Genes; D007908:Lens, Crystalline / Q000166:cytology*; D011401:Promoter Regions, Genetic; D011817:Rabbits; D014157:Transcription Factors / Q000032:analysis
D003459:Crystallins; D014157:Transcription Factors