A 12 megabase restriction map at the cystic fibrosis locus.
We have constructed a physical map of the chromosomal region containing the cystic fibrosis locus using seven DNA markers and pulsed-field gel electrophoresis methods. The map includes cleavage sites for 8 rare-cutting restriction enzymes and spans over 12 megabases (Mb) of DNA, with one unlinked probe covering an additional 5 Mb. To our knowledge, this is the largest segment of human DNA which has been restriction-mapped to date. We can identify thirteen putative HTF islands spaced at intervals of 0.3-3.2 Mb. The region between loci D7S8 and MET, where the CF gene lies, includes 1.4-1.9 Mb of DNA.
Fulton
T R
TR
Department of Human Genetics, Collaborative Research Inc., Bedford, MA.
Bowcock
A M
AM
Smith
D R
DR
Daneshvar
L
L
Green
P
P
Cavalli-Sforza
L L
LL
Donis-Keller
H
H
D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D003550:Cystic Fibrosis / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008040:Genetic Linkage; D005819:Genetic Markers / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D005819:Genetic Markers; D004247:DNA