The expression of Fc and complement receptors in young, adult and aged mice.
Age-dependent changes in the expression of Fc receptors (FcR) for different isotypes of immunoglobulins and receptors for C3b, C5b and C3bi fragments of complement on the membranes of peritoneal macrophages were studied with mice of different ages. An age-related increase in expression of Fc receptors for IgM, IgE, IgA, IgG2b and IgG3, and a decrease in the expression of Fc receptors for IgG1 was observed. The expression of FcR on macrophages of donors of different ages corresponded with Fc-receptor mediated phagocytosis. The highest number of C3b-binding macrophages was found in aged mice, in contrast to low numbers of C3bi-binding macrophages at this age. The percentage of C5b-binding macrophages was lowest in adult animals. We also observed effective inhibition of binding of the C3b component of complement by preincubation of macrophages with aggregated IgG and vice versa. These observations suggest that fluctuation in expression of Fc but not C receptors may be important to the generalized changes that occur in macrophage function during development and ageing.
Vĕtvicka
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Fornůsek
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Zídková
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D000375:Aging; D000818:Animals; D003182:Complement C5 / Q000276:immunology; D050776:Complement C5b; D005260:Female; D007136:Immunoglobulins / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D010587:Phagocytosis; D011951:Receptors, Complement / Q000032:analysis* / Q000276:immunology; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000032:analysis*
D003182:Complement C5; D007136:Immunoglobulins; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc; D050776:Complement C5b