Separation of serotonin from catechols by capillary zone electrophoresis with electrochemical detection.
Capillary zone electrophoresis with electrochemical detection is demonstrated with columns having only 9-microns inner diameter. Amperometric detection limits of 0.7 amol are reported for serotonin. The difficult problem of resolving serotonin and dopamine--two neurotransmitters of interest having similar electrophoretic mobilities--is addressed by chemical means to improve selectivity. These include buffer modification with 2-propanol and a system employing borate complexation of the catechol in combination with sodium dodecyl sulfate micelles.
Wallingford
R A
RA
Ewing
A G
AG
D002395:Catecholamines / Q000302:isolation & purification*; D004563:Electrochemistry; D004586:Electrophoresis; D012701:Serotonin / Q000302:isolation & purification*
D002395:Catecholamines; D012701:Serotonin