Regulation of ornithine decarboxylase in murine erythroleukemic cells by N,N'-diacetyl-1,6-hexanediamine and N-acetyl-1,6-hexanediamine.
The levels at which the ornithine decarboxylase gene is regulated in murine erythroleukemic cells treated with N,N'-hexamethylene bisacetamide (N,N' diacetyl-1,6-hexanediamine) and with N-acetyl-1,6-hexanediamine, the monoacetylated catabolite of this inducer of erythrodifferentiation, have been investigated. Although these two molecules are structurally related and both cause a decrease in ornithine decarboxylase activity, they did not similarly affect ornithine decarboxylase mRNA. In the presence of 4 mM N,N'-hexamethylenebisacetamide, at the concentration generally used to induce differentiation in these cells, a decreased steady-state level of ornithine decarboxylase mRNA, due to decreased gene transcription, was observed. In the presence of N-acetyl-1,6-hexanediamine the decrease in enzyme activity was shown to be associated with a decrease in the half-life of the enzyme in the absence of a change in gene transcription. These results show that in proliferating cultured cells changes in ornithine-decarboxylase-gene transcription can be uncoupled from changes in cell growth and that N-acetyl-1,6-hexanediamine only regulates ornithine decarboxylase expression post-transcriptionally.
Papazafiri
P
P
Laboratoire de Biologie et Génétique du Developpement, Unité associé 256, Université de Rennes, France.
Osborne
H B
HB
D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology* / Q000235:genetics; D051379:Mice; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D000081:Acetamides; D012333:RNA, Messenger; C050895:N-acetyl-1,6-diaminohexane; D009955:Ornithine Decarboxylase; C014026:hexamethylene bisacetamide