Rapid determination of total lipids in mosquitoes.
Total lipids in individual mosquitoes can be determined by extraction with chloroform-methanol followed by reaction with sulfuric acid and a vanillin-phosphoric acid reagent. By subtracting the lipids determined in a starved population, the method is suitable to establish variations of lipid reserves in field populations.
Van Handel
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Institute of Food and Agricultural Sciences, University of Florida, Vero Beach 32962.
D000818:Animals; D002138:Calibration; D009033:Culicidae / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D013053:Spectrophotometry
D008055:Lipids