Derivatives of epinephrine, norepinephrine, octopamine and histamine formed by homogenates of Trichostrongylus colubriformis, a nematode parasite of ruminants.
1. Radiolabeled epinephrine, norepinephrine, octopamine and histamine were introduced into homogenates of mixed sexes of the nematode Trichostrongylus colubriformis and the labeled derivatives formed during incubation were identified by HPLC. Vanillylmandelic acid (VMA), 4-hydroxy-3-methoxyphenyl glycol (HMPG) and metanephrine were formed from epinephrine. 2. VMA, HMPG and normetanephrine were produced from norepinephrine. 3. Octopamine was converted into norepinephrine, synephrine and epinephrine. 4. Imidazole-4-acetic acid, 1,4-methylimidazole acetic acid and acetylhistamine were formed from histamine. 5. Inhibitors of monoamine oxidase, catechol-O-methyltransferase and phenylethanolamine-N-methyltransferase were tested for their effects on the formation of certain of these derivatives, and the results suggested that these enzymes were active in the homogenate.
Frandsen
J C
JC
U.S. Department of Agriculture, Animal Parasite Research Laboratory, Auburn, Alabama 36931-0952.
Bone
L W
LW
D000818:Animals; D004837:Epinephrine / Q000378:metabolism*; D005260:Female; D006632:Histamine / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D009655:Octopamine / Q000378:metabolism*; D014254:Trichostrongylus / Q000378:metabolism*
D009655:Octopamine; D006632:Histamine; D009638:Norepinephrine; D004837:Epinephrine