title: A 12 megabase restriction map at the cystic fibrosis locus.
abstract: We have constructed a physical map of the chromosomal region containing the cystic fibrosis locus using seven DNA markers and pulsed-field gel electrophoresis methods. The map includes cleavage sites for 8 rare-cutting restriction enzymes and spans over 12 megabases (Mb) of DNA, with one unlinked probe covering an additional 5 Mb. To our knowledge, this is the largest segment of human DNA which has been restriction-mapped to date. We can identify thirteen putative HTF islands spaced at intervals of 0.3-3.2 Mb. The region between loci D7S8 and MET, where the CF gene lies, includes 1.4-1.9 Mb of DNA.
authors.affiliation: Department of Human Genetics, Collaborative Research Inc., Bedford, MA.
authors.forename: T R
authors.initials: TR
authors.lastname: Fulton
authors.forename: A M
authors.initials: AM
authors.lastname: Bowcock
authors.forename: D R
authors.initials: DR
authors.lastname: Smith
authors.forename: L
authors.initials: L
authors.lastname: Daneshvar
authors.forename: P
authors.initials: P
authors.lastname: Green
authors.forename: L L
authors.initials: LL
authors.lastname: Cavalli-Sforza
authors.forename: H
authors.initials: H
authors.lastname: Donis-Keller
mesh_terms: D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D003550:Cystic Fibrosis / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008040:Genetic Linkage; D005819:Genetic Markers / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
chemical_list: D005819:Genetic Markers; D004247:DNA