Surfactant phosphatidylcholine metabolism and surfactant function in preterm, ventilated lambs.
Preterm lambs were delivered at 138 days gestational age and ventilated for periods up to 24 h in order to study surfactant metabolism and surfactant function. The surfactant-saturated phosphatidylcholine pool in the alveolar wash was 13 +/- 4 mumol/kg and did not change from 10 min to 24 h after birth. Trace amounts of labeled natural sheep surfactant were mixed with fetal lung fluid at birth. By 24 h, 80% of the label had become lung-tissue-associated, yet there was no loss of label from phosphatidylcholine in the lungs when calculated as the sum of the lung tissue plus alveolar wash. De novo synthesized phosphatidylcholine was labeled with choline given by intravascular injection at 1 h of age. Labeled phosphatidylcholine accumulated in the lung tissue linearly to 24 h, and the labeled phosphatidylcholine moved through lamellar body to alveolar pools. The turnover time for alveolar phosphatidylcholine was estimated to be about 13 h, indicating an active metabolic pool. A less surface-active surfactant fraction recovered as a supernatant after centrifugation of the alveolar washes at 40,000 x g increased from birth to 10 min of ventilation, but no subsequent changes in the distribution of surfactant phosphatidylcholine in surfactant fractions occurred. The results were consistent with recycling pathway(s) that maintained surface-active surfactant pools in preterm ventilated lambs.
Jobe
A H
AH
Department of Pediatrics, Harbor-UCLA Medical Center, UCLA School of Medicine, Torrance.
Ikegami
M
M
Seidner
S R
SR
Pettenazzo
A
A
Ruffini
L
L
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis; D002250:Carbon Radioisotopes; D005333:Fetus / Q000502:physiology; D007457:Iodine Radioisotopes; D010713:Phosphatidylcholines / Q000032:analysis / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D011663:Pulmonary Surfactants / Q000032:analysis / Q000378:metabolism*; D012121:Respiration, Artificial; D012756:Sheep / Q000502:physiology*; D013500:Surface Tension; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D007457:Iodine Radioisotopes; D010713:Phosphatidylcholines; D010743:Phospholipids; D011663:Pulmonary Surfactants; D014316:Tritium