Generation of PM2 DNA breaks in the course of reduction of chromium(VI) by glutathione.
The carcinogen chromate is efficiently taken up and reduced to chromium(III) compounds by various biological systems. To test the possible DNA damage induced in the course of chromium(VI) reduction, we used a combination of chromate with the reductant glutathione (GSH) as well as a green complex of chromium(V), which is formed in the reaction of chromate with GSH. The combination of chromate and glutathione was found to cause single-strand breaks in supercoiled circular DNA of the bacteriophage PM2. The green chromium(V) complex Na4(GSH)4Cr(V).8H2O, prepared from chromate and glutathione, also cleaved supercoiled PM2 DNA. No DNA-degrading effects were observed with either chromate or the final product of the reaction with GSH, a purple anionic chromium(III) GSH complex. The nature of the buffering agents revealed a strong influence on the extent of DNA strand breaks produced by chromate and GSH. A variation of the GSH concentration in the reaction with chromate and PM2 DNA, performed in sodium phosphate-buffered solutions showed an initial increase in the number of strand breaks at GSH concentrations up to 1 mM followed by a decline at higher GSH concentrations. Since neither chromate, when administered individually, nor the final product of chromium(VI) reduction, the purple chromium(III) GSH complex, produced any detectable DNA cleavage, the critical steps leading to DNA strand breaks occur in the course of the conversion of chromium(VI) to chromium(III) by GSH, the most abundant intracellular low molecular thiol. Moreover, the demonstration that DNA cleavage is induced in the presence of the chromium(V) complex identifies chromium(V) as the oxidation state of the metal, which is involved in the steps leading to DNA-damaging effects of chromate.
Kortenkamp
A
A
Universität Bremen, F.R.G.
Ozolins
Z
Z
Beyersmann
D
D
O'Brien
P
P
D001435:Bacteriophages; D002021:Buffers; D002857:Chromium / Q000378:metabolism / Q000633:toxicity*; D004249:DNA Damage; D004278:DNA, Superhelical / Q000187:drug effects*; D004279:DNA, Viral / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D005978:Glutathione / Q000378:metabolism*; D066298:In Vitro Techniques; D010084:Oxidation-Reduction
D002021:Buffers; D004278:DNA, Superhelical; D004279:DNA, Viral; D002857:Chromium; D005978:Glutathione