Monocyte long-term cultivation on microvascular endothelial cell monolayers: morphologic and phenotypic characterization and comparison with monocytes cultured on tissue culture plastic.
Human monocytes were cultured on monolayers of a newly established microvascular endothelial cell strain. As compared to monocytes cultured on plastic, these endothelium-"derived" monocytes (EDM) showed distinct morphology, higher motility, and different antigen-expression pattern for several surface markers, as detected by cytofluorimetry. The MO-1- and the Leu-M1-marker were maintained on EDMs while they were lost on plastic-cultured cells. The MAX 1-26-termed markers failed to increase on EDMs, in contrast to plastic-cultured monocytes. For seven additional markers, expression after two weeks in vitro was higher on EDMs than on plastic-cultured monocytes. Functionally EDMs showed typical monocyte/macrophage behavior and were easily removable from the culture system for further experimentation. Our data suggest that monocytes cultured on microvascular endothelial cells are maintained for several weeks in a more physiologic state than monocytes cultured on plastic.
Schumann
R R
RR
Forschunginstitut Borstel, Institute for Experimental Biology and Medicine, West Germany.
van der Bosch
J
J
Rüller
S
S
Ernst
M
M
Schlaak
M
M
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D002448:Cell Adhesion; D002449:Cell Aggregation; D002454:Cell Differentiation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D009000:Monocytes / Q000166:cytology*; D010969:Plastics; D011817:Rabbits
D000943:Antigens, Differentiation; D006680:HLA Antigens; D010969:Plastics