High-performance liquid chromatographic analysis of steroid hormones.
In the present work, a practical, rapid, reliable and isocratic reversed-phase high-performance liquid chromatographic (HPLC) method is described for the qualitative and quantitative analysis of estriol, estradiol-17 beta, estrone, testosterone, and progesterone. Chromatographic separation is complete in 16 min using a mobile phase of 50% acetonitrile (v/v) in water. The order of elution is estriol, testosterone, estradiol-17 beta, estrone, and progesterone; retention times are 2.5, 5.5, 5.6, 6.9, 16.3 min, respectively. Absorbance maxima of individual steroids is the limiting factor in quantitative determination. The recommended wavelengths for UV monitoring are E3 214, E2 280, T 254, E1 214, and P4 254 nm.
Erkoç
F U
FU
Turkish Atomic Energy Authority, Lalahan Nuclear Research Institute of Animal Health, Ankara.
Ozsar
S
S
Güven
B
B
Kalkandelen
G
G
Uğrar
E
E
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004967:Estrogens / Q000032:analysis; D006728:Hormones / Q000032:analysis*; D011374:Progesterone / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013739:Testosterone / Q000032:analysis
D004967:Estrogens; D006728:Hormones; D013739:Testosterone; D011374:Progesterone