A time-resolved fluorescence anisotropy study of bilayer membranes containing alpha-tocopherol.
Rotational mobility in fluid phase dipalmitoylphosphatidylcholine unilamellar vesicles containing alpha-tocopherol has been studied by time-resolved anisotropy measurements of fluorescence from a diphenylhexatriene-phosphatidylcholine conjugate. The results are analysed using a simple wobbling-in-cone model. The diphenylhexatriene probe shows an increasing order parameter and more restricted wobbling with increasing alpha-tocopherol content of the membrane. The diffusional rate for wobbling was found not to change significantly.
Bisby
R H
RH
Department of Biological Sciences, University of Salford, UK.
Birch
D J
DJ
D005454:Fluorescence Polarization; D008051:Lipid Bilayers; D008560:Membrane Fluidity; D014810:Vitamin E
D008051:Lipid Bilayers; D014810:Vitamin E