title: Isoenzyme patterns of protein kinase C and a phospholipid-dependent but Ca2+-inhibited enzyme fraction in the crude extracts of different tissues.
abstract: We compared the protein kinase C isoenzyme patterns of crude extracts of rabbit brain, cerebellum, spleen, thymus and human and pig granulocytes. The isoenzymes were fractionated by hydroxyapatite chromatography and the protein kinase C activity was determined with a synthetic oligopeptide substrate. In the extracts of several tissues we also observed an enzyme fraction which was activated by phosphatidylserine + diacylglycerol but inhibited by Ca2+.
authors.affiliation: 1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Faragó
authors.forename: G
authors.initials: G
authors.lastname: Farkas
authors.forename: G
authors.initials: G
authors.lastname: Mészáros
authors.forename: L
authors.initials: L
authors.lastname: Buday
authors.forename: F
authors.initials: F
authors.lastname: Antoni
authors.forename: J
authors.initials: J
authors.lastname: Seprödi
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D002845:Chromatography / Q000379:methods; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000037:antagonists & inhibitors; D010718:Phosphatidylserines / Q000494:pharmacology; D011493:Protein Kinase C / Q000032:analysis* / Q000037:antagonists & inhibitors; D011817:Rabbits; D013154:Spleen / Q000201:enzymology; D013552:Swine; D013950:Thymus Gland / Q000201:enzymology; D014020:Tissue Extracts / Q000032:analysis
chemical_list: D004075:Diglycerides; D007527:Isoenzymes; D010718:Phosphatidylserines; D014020:Tissue Extracts; D011493:Protein Kinase C; D002118:Calcium