Interaction of antiprogestins with progesterone receptors in rat uterus.
Cytosolic and nuclear progesterone receptors (PRc and PRn) under antiprogestin treatment were measured in rat deciduoma and compared with values for contralateral (nondeciduomatous) rat uterine tissue. Uterine PRc and PRn of the progesterone treated group were 101 +/- 8.7 and 4770 +/- 590 fmol/mg DNA respectively. After treatment with antiprogestins STS-557, 5 alpha-DNE, (5 alpha-dihydronorethisterone), 5 alpha-DNG (5 alpha-dihydronorgestrel), RU-22092 and RU-16556, PRc in the nondeciduomatous control horn ranged from 127 to 377 fmol/mg DNA and PRn from 2785 to 17925 fmol/mg DNA. In the decidual tissue, PRc decreased significantly (4.6 +/- 0.8 fmol/mg DNA) on 5 alpha-DNG treatment as compared with the progesterone alone treatment group (147 +/- 3.8). PRn in decidual tissue also decreased maximally on 5 alpha-DNG treatment. These results suggest that the interaction of antiprogestins may not be identical in control uterine tissue and in deciduoma.
Vij
U
U
Department of Reproductive Biology, All India Institute of Medical Sciences, New Delhi.
Murugesan
K
K
Kalita
J C
JC
Farooq
A
A
D000818:Animals; D005260:Female; D009277:Nandrolone / Q000031:analogs & derivatives / Q000494:pharmacology; D011372:Progestins / Q000037:antagonists & inhibitors*; D051381:Rats; D011980:Receptors, Progesterone / Q000378:metabolism*; D013256:Steroids / Q000494:pharmacology; D014599:Uterus / Q000378:metabolism*
D011372:Progestins; D011980:Receptors, Progesterone; D013256:Steroids; C023635:dienogest; D009277:Nandrolone