title: Two-dimensional structure of phospholipase induced crystals of Ca2+-ATPase from sarcoplasmic reticulum.
abstract: A two-dimensional projection map was computed of the Ca2+-ATPase molecules in sarcoplasmic reticulum, isolated from rabbit skeletal muscle. Crystalline arrays of Ca2+-ATPase molecules were formed by incubating the membrane vesicles with phospholipase A2 and dialysing against Tris/HCl buffer. Ca2+-ATPase molecules appear as quasi-triangular blobs in the projection map and seem to form dimers. The projection map seems to indicate an enzyme conformation somewhat similar to vanadate-induced crystals but different from lanthanide-induced crystals of Ca2+-ATPase.
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases; D003461:Crystallography; D010741:Phospholipases A; D054467:Phospholipases A2; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D010741:Phospholipases A; D054467:Phospholipases A2; D000252:Calcium-Transporting ATPases