Binding sites for atrial natriuretic factor (ANF) in brain: alterations in Brattleboro rats.
Binding sites for atrial natriuretic factor (ANF-28) were analyzed in discrete brain areas of Brattleboro rats with hereditary diabetes insipidus and Long-Evans (LE) controls by quantitative autoradiography. The maximum binding capacity (Bmax) and affinity constant (Ka) for 125I-ANF-28 were elevated significantly in the subfornical organ of Brattleboro rats compared to matched LE controls. In contrast, values for Bmax and Ka for 125I-ANF-28 binding in choroid plexus and area postrema were similar for rats of the two strains. These findings are consistent with a selective upregulation of ANF-28 binding sites in the subfornical organ of Brattleboro rats which exhibit a profound disturbance in body fluid homeostasis. These alterations in ANF-28 binding sites in the subfornical organ may represent a compensatory response to the absence of vasopressin in the Brattleboro rat.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001345:Autoradiography; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D003919:Diabetes Insipidus / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D051381:Rats; D011910:Rats, Brattleboro; D013356:Subfornical Organ / Q000378:metabolism
D007457:Iodine Radioisotopes; D009320:Atrial Natriuretic Factor