Prevention of adriamycin-induced interphase death by 3-aminobenzamide and nicotinamide in a human promyelocytic leukemia cell line.
Adriamycin caused significant interphase death in HL-60 cells during six hours of incubation, which was abolished by the poly(ADP-ribose) polymerase inhibitors, 3-aminobenzamide or nicotinamide. Neither agent changed adriamycin uptake by HL-60 cells. Although 3-aminobenzamide did not alter the number of DNA strand breaks caused by adriamycin, it prevented adriamycin-induced depletion of intracellular NAD+ and ATP, and maintained energy charge. These findings suggest that the activation of poly(ADP-ribose) synthesis plays an important role in the adriamycin-induced interphase death of proliferating HL-60 cells.
D001549:Benzamides / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004317:Doxorubicin / Q000494:pharmacology*; D006801:Humans; D007399:Interphase / Q000187:drug effects*; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009536:Niacinamide / Q000494:pharmacology*
D001549:Benzamides; D008769:Methylnitronitrosoguanidine; D009536:Niacinamide; D004317:Doxorubicin; C025160:3-aminobenzamide