[Lipid peroxidation as a major factor in the modification of the catalytic function of Ca-ATPase of the sarcoplasmic reticulum in hypercholesterolemia].
A comparative study of the effect of an experimental hypercholesterolemia and in vitro induced lipid peroxidation (LPO) on the temperature dependence of the activity of sarcoplasmic reticular Ca-ATPase from rabbit skeletal muscle (SR) has been performed. A control Arrhenius plot of ATPase activity determined in the presence of alamethicin was characterized by discontinuity in the 20 degrees C area. Both in vitro induced LPO and hypercholesterolemia resulted in a shift of discontinuity to 30 degrees C area. The replacement of lipid Ca-ATPase membrane environment by egg yolk lecithin did not affect the temperature dependence of the activity in control SR and failed to restore the original nature of the Arrhenius plot for Ca-ATPase modified by hypercholesterolemia or the in vitro induced LPO.
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006937:Hypercholesterolemia / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000201:enzymology; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D014810:Vitamin E / Q000494:pharmacology
D008054:Lipid Peroxides; D014810:Vitamin E; D000252:Calcium-Transporting ATPases
Perekisnoe okislenie lipidov kak osnovnaia prichina modifikatsii kataliticheskoĭ funktsii Ca-ATFazy sarkoplazmaticheskogo retikuluma pri giperkholesterinemii.