title: Contractile failure and high-energy phosphate turnover during hypoxia: 31P-NMR surface coil studies in living rat.
abstract: Cardiac failure appears rapidly during severe hypoxia and precedes a substantial reduction in adenosine triphosphate content. Reduced adenosine triphosphate turnover, in the presence of nearly normal content, may be the metabolic basis for contractile failure during hypoxia. To measure both the myocardial content and the turnover rates of high-energy phosphate compounds during hypoxia, we performed 31P-nuclear magnetic resonance studies by placing a surface coil directly over the left ventricle in intubated rats that were instrumented for hemodynamic measurements and ventilated with either 21, 10, or 8% O2. Normoxia produced a hemodynamic and metabolic steady state for 4 hours and hypoxia for at least 60 minutes. Under normoxic ventilation (n = 10, mean +/- SD), the arterial PO2 was 96 +/- 14, pH 7.38 +/- 0.11, and systolic blood pressure 96 +/- 8 mm Hg; under hypoxic ventilation with 10% O2 (n = 5), the arterial PO2 was 57 +/- 10, pH 7.39 +/- 0.09, and systolic pressure 68 +/- 10; and under hypoxic ventilation with 8% O2 (n = 5), the PO2 was 52 +/- 7, pH 7.37 +/- 0.04, and systolic pressure 51 +/- 4. Hypoxic ventilation with 10 or 8% O2 decreased the creatine phosphate content from 51.4 +/- 5.4 mumol/g dry wt to 39.3 +/- 5.4 and 45.6 +/- 4.1 and depressed adenosine triphosphate slightly from 25.0 mumol/g dry wt to 21.8 +/- 2.1 and 21.9 +/- 1.0, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D006439:Hemodynamics; D000860:Hypoxia / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D010100:Oxygen / Q000097:blood; D010725:Phosphocreatine / Q000378:metabolism; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D000251:Adenosine Triphosphatases; D010100:Oxygen