title: Activities involved in base excision repair of bacteriophage T4 and lambda DNA in vivo.
abstract: The in vivo excision repair functions of Escherichia coli exonuclease III and 3-methyladenine DNA glycosylase I, and bacteriophage T4 pyrimidine dimer-DNA glycosylase were investigated. Following exposure of bacteriophage T4 or lambda to methyl methanesulfonate or ultraviolet irradiation, survival was determined by plating on E. coli have various genetic backgrounds. Although exonuclease III was shown to participate in base excision repair initiated by 3-methyladenine DNA glycosylase I, it had no detectable role in base excision repair initiated by the T4 pyrimidine dimer-DNA glycosylase. Despite its 3' apurinic/apyrimidinic endonuclease activity in vitro, T4 pyrimidine dimer-DNA glycosylase, even in large quantities, did not complement mutants defective in exonuclease III in the repair of apurinic sites generated by 3-methyladenine DNA glycosylase I in vivo.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D045647:DNA Glycosylases; D004260:DNA Repair; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005838:Genotype; D007700:Kinetics; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D010957:Plasmids; D013604:T-Phages / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; C021565:3-methyladenine-DNA glycosylase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; C036401:deoxyribopyrimidine endonucleosidase