title: Birth of calves after in vitro fertilisation using laparoscopy and rabbit oviduct incubation of zygotes.
abstract: The infertility of many cows could be treated by in vitro fertilisation. In the present study laparoscopy was utilised to recover the in vivo matured oocytes from the ovary of a standing donor. After the capacitation of fresh semen with high ionic strength medium and in vitro fertilisation, a rabbit oviduct was employed as an incubator for four to five days, in order to obtain sufficiently aged embryos to be transferred to the uterus of a recipient. Using surgical or non-surgical transfer six calves were obtained.
mesh_terms: D000818:Animals; D002417:Cattle; D004624:Embryo Transfer / Q000662:veterinary*; D005307:Fertilization in Vitro / Q000662:veterinary*; D010535:Laparoscopy / Q000662:veterinary*; D009865:Oocytes / Q000637:transplantation; D010057:Oviducts / Q000601:surgery; D011817:Rabbits