title: Endotoxin but not malnutrition promotes bacterial translocation of the gut flora in burned mice.
abstract: Previously we have shown that under certain conditions, bacteria can pass through the intact epithelial mucosa to the mesenteric lymph nodes (MLN), liver, spleen, and bloodstream to cause infection, a process termed bacterial translocation. To extend these studies, we determined the influence of protein malnutrition and endotoxemia on bacterial translocation in burned (25% TBSA) and unburned mice. The results of these experiments documented that protein malnutrition did not promote bacterial translocation from the gut in either burned or unburned animals, although it did disrupt the normal indigenous gut flora. In contrast, a nonlethal dose of endotoxin (IP) promoted bacterial translocation to the mesenteric lymph nodes in burned and unburned mice, but only in burned mice did the bacteria translocate from the gut to other systemic organs (p less than 0.01). Furthermore, the mortality rate of mice receiving only endotoxin or burn was less than 10%, while the combination of endotoxin plus a thermal injury increased the mortality rate to 100% (p less than 0.01). These studies support the concept that bacteria may translocate from the gut to other organs and be a potential source of lethal infections after thermal injury.
mesh_terms: D000818:Animals; D018407:Bacterial Physiological Phenomena; D002056:Burns / Q000503:physiopathology*; D004731:Endotoxins / Q000097:blood*; D005260:Female; D007422:Intestines / Q000382:microbiology*; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D051379:Mice; D008813:Mice, Inbred ICR; D011502:Protein-Energy Malnutrition / Q000503:physiopathology*; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000382:microbiology
chemical_list: D004731:Endotoxins