Stimulation of synaptosomal ATP-dependent Ca2+-uptake by N-ethylmaleimide.
Treatment of rat brain synaptosomal lysate with N-ethylmaleimide (NEM) was found to stimulate ATP-dependent Ca2+-uptake. This Ca2+-uptake stimulation was blocked by dithioerythritol (DTE), mitochondrial inhibitors oligomycin and sodium azide, but not by vanadate, an inhibitor of plasma membrane Ca2+ pump. Maximal stimulation of Ca2+-uptake was observed at a NEM/protein ratio of 0.1 mumole/0.5-1.0 mg. On fractionation, it was found that NEM did not affect synaptic plasma membrane Ca2+-uptake, but almost doubled it in synaptic mitochondria.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001386:Azides / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D019810:Sodium Azide; D013574:Synaptosomes / Q000378:metabolism*; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D001386:Azides; D009840:Oligomycins; D014639:Vanadium; D014638:Vanadates; D000255:Adenosine Triphosphate; D019810:Sodium Azide; D000252:Calcium-Transporting ATPases; D005033:Ethylmaleimide; D002118:Calcium