Potential anticoagulant activity of lipocortins and other calcium/phospholipid binding proteins.
Thrombin generation was determined in the presence of phospholipids, coagulation factors Xm, Vm and II (prothrombin), calcium, and various calcium/phospholipid bindings proteins, including lipocortins I and II, 35 kDa calelectrin, and 32.5 kDa endonexin. All of these proteins induced a dose-dependent inhibition of thrombin generation similar to the inhibition of pig pancreas phospholipase A2. It is suggested that the ability of lipocortins and other related proteins to interact with anionic phospholipids in the presence of Ca++ is responsible for both their anticoagulant and anti-phospholipase A2 activity.
D000818:Animals; D017304:Annexin A5; D017302:Annexins; D001777:Blood Coagulation / Q000187:drug effects*; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D007526:Isoelectric Point; D008099:Liver / Q000032:analysis; D008168:Lung / Q000032:analysis; D010740:Phospholipases / Q000037:antagonists & inhibitors; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D013552:Swine; D013917:Thrombin / Q000378:metabolism
D017304:Annexin A5; D017302:Annexins; D002135:Calcium-Binding Proteins; D006023:Glycoproteins; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D013917:Thrombin