A chicken leukocyte common antigen: biochemical characterization and ontogenetic study.
A monoclonal antibody, designated CL-1, was produced by immunizing mice with chicken peripheral blood lymphocytes (PBL) and selected in an indirect immunofluorescence assay. CL-1 characterizes a cell surface antigen expressed on all chicken hemopoietic cells except mature erythrocytes. Cells stained by CL-1 were detected at early stages of embryonic development in the various hemopoietic compartments. CL-1 reacts with two chicken PBL proteins of 200 kDa and 215 kDa which are probably the homologues of the T-200 and B-220 antigens previously described in the mouse. Moreover, CL-1 does not react with quail hemopoietic tissues and can be useful in experiments using quail-chick chimeras to study the ontogeny of the hemopoietic system.
D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens; D003370:Coturnix; D004625:Embryo, Nonmammalian / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens / Q000032:analysis*; D017493:Leukocyte Common Antigens; D008214:Lymphocytes / Q000276:immunology*; D008970:Molecular Weight; D009928:Organ Specificity
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D017493:Leukocyte Common Antigens