Electron microscopic studies of giant nucleus-like structure formed by lambda DNA introduced into the cytoplasm of Xenopus laevis fertilized eggs and embryos.
When bacteriophage lambda DNA was injected into the cytoplasm of the fertilized egg of Xenopus laevis, giant nucleus-like structures were assembled around the injected DNA. These nucleus-like structures survived during cleavage and were partitioned into blastomeres at the blastula stage. The nucleus-like structures formed in the uncleaved fertilized eggs and the blastula cells were both surrounded by a bilayer nuclear membrane with nuclear pore complexes. The ultrastructural features of the lambda DNA-induced nucleus-like structure were considerably different from those of the normal blastula nucleus: although the nuclear pore complexes appeared to be normal, the 'nucleoplasm' was much too homogeneous as compared with that of the normal nucleus.
D000818:Animals; D010582:Bacteriophage lambda; D001755:Blastocyst / Q000648:ultrastructure*; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000648:ultrastructure*; D002970:Cleavage Stage, Ovum / Q000648:ultrastructure*; D004279:DNA, Viral / Q000378:metabolism*; D008845:Microinjections; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000648:ultrastructure; D014982:Xenopus laevis
D004279:DNA, Viral