Maintained allogeneic pregnancy in rats depleted of T cytotoxic/suppressor cells by OX8 monoclonal antibody treatment.
It has been suggested that CD8 positive suppressor T-cells might be of importance in the non-rejection of the fetus. In the present investigation allogeneically pregnant (Lewix x DA) rats were subjected to in vivo treatment with monoclonal OX8 antibodies, reactive with the rat equivalent of CD8 receptor. This treatment protocol drastically reduced the numbers of OX8 positive cells in spleens and para-aortic lymph nodes. On the day of delivery these rats, together with normal IgG treated controls, were dissected and analysed for effects on: (1) fetal survival; (2) weight and immunohistology of spleens and para-aortic lymph nodes; (3) total numbers of IgM- and IgG-secreting cells within these organs. The OX8 treated rats passed through pregnancy as successfully as did the controls. Both groups showed the same type of pregnancy-induced changes in their lymphoid organs, including dramatic growth of the para-aortic lymph nodes and increase in Ig-secretors within both spleen and para-aortic lymph nodes. This pattern was the same in all pregnant rats investigated, including untreated syngeneically mated Lewis rats. It is concluded that OX8 positive T "suppressor" cells do not play an important role in the maintenance of the fetal-placental unit.
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D007519:Isoantigens / Q000276:immunology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007519:Isoantigens