Mechanism of radiosensitizing effect of chloride ion on E. coli.
Cells of E. coli capable of repairing DNA damage are sensitized to radiation in the presence of NaCl. However, the enhanced radiolethality was suppressed by the addition of compounds such as an amino acid to the irradiation buffer. The protective efficiencies of these compounds depend on their reactivities with Cl-.2 or OH.. ATP synthesis in the cells irradiated in the presence of NaCl was severely inhibited depending on the dose of irradiation. This reduced rate of ATP synthesis can account for the inhibition of protein, RNA and DNA synthesis in the irradiated cells with NaCl.
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000596:Amino Acids / Q000494:pharmacology; D010584:Bacteriophage phi X 174 / Q000502:physiology; D002712:Chlorides / Q000494:pharmacology*; D002852:Chromatography, Ion Exchange; D004260:DNA Repair; D004926:Escherichia coli / Q000187:drug effects / Q000528:radiation effects*; D008433:Mathematics; D011836:Radiation Tolerance / Q000187:drug effects*; D012965:Sodium Chloride / Q000494:pharmacology; D014779:Virus Replication / Q000528:radiation effects
D000596:Amino Acids; D002712:Chlorides; D012965:Sodium Chloride; D000255:Adenosine Triphosphate