Tissue culture experiments on the vegetative micropropagation of oak.
Excised parts of juvenile green stem, seedlings growing out from unripe acorns and epicotyl segments of seedlings proved to be the best inocula for vegetative micropropagation under the given conditions. By means of interactions between mineral nutrients and supplementary regulators, callus, shoots (shoot sprouting) and regeneration of plantlets were induced from such explants. In the morphology of regeneration, the type of oak inocula rather than the basal medium played a primary role in the experiments described, the latter being just an influencing factor. No regeneration could be induced from resting buds of shoot, meristem tips of axillary buds, anthers or pollen, under the experimental conditions applied. Results obtained, in a further developed form, seem to be suitable for vegetative micropropagation of oaks.
D003470:Culture Media; D008722:Methods; D014197:Trees / Q000254:growth & development*
D003470:Culture Media