[Determination, by the immunogold method, of T lymphocyte sub-populations during HIV infections (LAV/HTLV III)].
This study analyzes a method of indirect marking of T lymphocytes subpopulations using a 2nd antibody coupled to colloidal gold particles (Immunogold). There is an excellent correlation between this technique and the classical technique of indirect immunofluorescence. Immunogold enables an easy and rapid count of lymphocytes CD4+ and CD8+ during infections secondary to HIV virus (LAV/HTLV III). The permanent nature of the preparations and the cytological control make this method an interesting tool for the diagnosis and follow-up of patients suffering from AIDS, ARC or only asymptomatic seropositive individuals.
D000386:AIDS-Related Complex / Q000097:blood; D000163:Acquired Immunodeficiency Syndrome / Q000097:blood*; D005455:Fluorescent Antibody Technique; D006046:Gold; D006801:Humans; D007150:Immunohistochemistry; D007958:Leukocyte Count / Q000379:methods; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology*; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology*; D050378:T-Lymphocytes, Regulatory / Q000473:pathology*
D006046:Gold
Détermination par la méthode immunogold des sous-populations lymphocytaires T au cours des infections par le virus HIV (LAV/HTLV III).