[Properties of DNA-(cytosine-5-)-methyltransferase in the brain].
Beef brain DNA-(cytosine-5-)-methyltransferase was partially purified by chromatography on Ultrogel AcA34 and Dyematrex Blue A. The purification was of 360 times and the recovery of 75%. The pH optimum of the reaction is 7.6 NaCl inhibits double stranded DNA methylation, but stimulates single stranded DNA methylation up to 50 mM, before inhibiting. EDTA (1 mM) and MgCl2 (4 mM) stimulate DNA methylation. Polyamines inhibit the reaction.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002413:Cations, Divalent; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
D002413:Cations, Divalent; D004492:Edetic Acid; D004248:DNA (Cytosine-5-)-Methyltransferases
Propriétés de l'ADN-(cytosine-5-)-méthyltransférase de cerveau.