Differences in immunological susceptibility to cadmium toxicity between two rat strains as demonstrated with cell biological methods. Effect of cadmium on DNA synthesis of thymus lymphocytes.
When 2 inbred rat strains, the Brown-Norway rat and the Lewis rat were exposed to the same amount of CdCl2 for 15 days, a completely different immunological reaction pattern could be demonstrated. Despite the same amount of intrathymic cadmium in both strains, the Brown-Norway rat showed a significant decrease in thymocytes in the S-phase and a significant increase of thymocytes in the G2 phase and mitosis, in contrast with findings in the Lewis rats. A new method for estimating subtle forms of thymus atrophy showed a slight decrease in the number of the smallest thymocytes in the Brown-Norway rat after exposure to cadmium, in contrast with that in the Lewis rat. Evidence is presented that the approximately 1.7 times larger number of thymocytes/mg thymus in the Lewis rat, compared to the Brown-Norway rat, as well as the approximately 2.5 times lower proliferation rate of the thymocytes, and an approximately 1.5 times higher metallothionein content of the thymus medulla epithelial cells in the Lewis rat, might be responsible for the observed difference in toxicity. The zinc content of the thymus was not significantly decreased by exposure to CdCl2, and did not differ significantly between both strains.
D000818:Animals; D002104:Cadmium / Q000032:analysis / Q000633:toxicity*; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D005434:Flow Cytometry; D007120:Immunochemistry; D008099:Liver / Q000032:analysis; D008668:Metallothionein / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects* / Q000378:metabolism; D015032:Zinc / Q000032:analysis
D002104:Cadmium; D004247:DNA; D008668:Metallothionein; D015032:Zinc