Increased adhesion to fibronectin and Mo-1 expression by diabetic monocytes.
As excessive monocyte adhesion to blood vessel wall could produce endothelial cell injury, we undertook comparative studies on the monocyte adhesiveness in insulin-dependent diabetics with vascular complications (n = 26) and healthy, normal subjects (n = 36). In the diabetic group, the extent of monocyte adhesion on fibronectin- and autologous plasma-coated surfaces was significantly increased compared with that in the control group (p less than 0.01 on both types of surfaces). Monocytes adhesion to the plasma-coated surface, but not to the fibronectin-coated surface, could be inhibited by monoclonal anti-Mo-1 antibody in a dose-dependent manner. For diabetic monocyte adhesion, a higher amount of anti-Mo-1 antibody was required to produce a similar extent of inhibition as observed with control monocytes. This indication of increased Mo-1 expression on diabetic monocytes was further confirmed by analyzing the fluorescence intensity of monocytes labeled with the antibody anti-Mo-1. The results of the present study suggest that diabetic monocytes have increased adhesiveness as the result of increased expression of fibronectin and Mo-1 receptors.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D002448:Cell Adhesion; D003182:Complement C5 / Q000031:analogs & derivatives / Q000032:analysis; D015937:Complement C5a, des-Arginine; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D003925:Diabetic Angiopathies / Q000503:physiopathology*; D005353:Fibronectins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005780:Gelatin / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D016177:Macrophage-1 Antigen; D009000:Monocytes / Q000378:metabolism*; D011951:Receptors, Complement / Q000032:analysis; D017463:Receptors, Complement 3b; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000032:analysis
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D003182:Complement C5; D015937:Complement C5a, des-Arginine; D005353:Fibronectins; D006023:Glycoproteins; D016177:Macrophage-1 Antigen; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D005780:Gelatin