The effect of dam methylation on the expression of glnS in E. coli.
The wild type glnS promoter contains a dam methylation site. In dam strains, the expression of glnS is enhanced 2.6-fold. A mutated form of the promoter has been isolated in which the dam methylation site is lost. Expression of this promoter is insensitive to dam methylation.
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005975:Glutamate-tRNA Ligase / Q000235:genetics*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D009154:Mutation; D011401:Promoter Regions, Genetic; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D014158:Transcription, Genetic
D004269:DNA, Bacterial; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D000604:Amino Acyl-tRNA Synthetases; D005975:Glutamate-tRNA Ligase