Interleukin 2 mediates an alteration in the T200 antigen expressed on activated B lymphocytes.
We have examined the effect of exogenous IL 2 on cell surface antigen expression in LPS/dextran sulfate-activated murine B cells with the use of a panel of fluorescein-conjugated lectins. Elevated binding of the lectins PNA and SBA to activated B cells was found to be mediated by IL 2-containing supernatants from stimulated EL4 cells as well as by recombinant IL 2. These lectins have specificity for terminal beta-(1-3)-N-acetylgalactosaminyl residues; thus, the quantity or accessibility of these moieties is mediated by IL 2 in activated B lymphocytes. PNA binding in all strains tested, regardless of MHC or background genes, was found to be elevated fivefold to 15-fold by exogenous IL 2. To observe this effect, IL 2 must be added during the first 24 hr of culture. Based on anti-Thy-1 + complement depletion studies, T cells were not required, suggesting a direct effect of IL 2 on B cells. The glycoprotein responsible for this elevated binding of PNA has an Mr of approximately 220K and by immunodepletion was shown to belong to the T200 (Ly-5) family of cell surface antigens. These data demonstrate that exogenous IL 2 can mediate alterations in T200 expression on activated B cells that may be related to IL 2-driven modulation of B cell proliferation and/or differentiation.
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology*; D007376:Interleukin-2 / Q000008:administration & dosage / Q000502:physiology*; D037102:Lectins; D017493:Leukocyte Common Antigens; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D019887:Peanut Agglutinin; D011975:Receptors, Mitogen / Q000502:physiology; D011994:Recombinant Proteins / Q000008:administration & dosage; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; D006649:Histocompatibility Antigens; D007376:Interleukin-2; D037102:Lectins; D019887:Peanut Agglutinin; D011975:Receptors, Mitogen; D011994:Recombinant Proteins; C038223:peanut agglutinin receptor; D017493:Leukocyte Common Antigens