title: Syrinx 2A: an improved lambda phage vector designed for screening DNA libraries by recombination in vivo.
abstract: The Syrinx 2A phage and pi AN13 plasmid were designed for screening of DNA libraries by homologous recombination in vivo. Syrinx 2A carries multiple cloning sites and a recently identified lambda gene, rap (recombination adept with plasmid), required for efficient phage-plasmid recombination. We describe a rapid, reliable, and technically easy method to screen Syrinx 2A libraries, expand the resulting phage-plasmid cointegrates, and subclone plasmid in as little as 2 days. Recombination screening allows one specific member of a closely related multigene family to be isolated selectively.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant / Q000032:analysis*; D005822:Genetic Vectors; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D005810:Multigene Family; D010957:Plasmids; D011995:Recombination, Genetic
chemical_list: D004274:DNA, Recombinant; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains