title: Hypoxic incubation leads to concerted changes of carbonic anhydrase activity and 2.3 DPG concentration of chick embryo red cells.
abstract: Red cell carbonic anhydrase activity, 2.3 DPG concentration, and activities of three key enzymes controlling DPG metabolism (PK, PFK and DPGM) were measured in normoxic and hypoxic (incubation in 13.5% O2) chick embryos. In normoxia 2.3 DPG concentration and carbonic anhydrase activity begin to increase by the third week of incubation. Hypoxia induces a rise of 2.3 DPG concentration and carbonic anhydrase activity by Day 8 of development, i.e., 1 week earlier. Since during normal development chick embryos become hypoxic in the third week of incubation, the results suggest that PO2 has a controlling influence on the timing of differentiation events of definitive embryonic red cells.
mesh_terms: D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000097:blood*; D002454:Cell Differentiation; D002642:Chick Embryo; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000502:physiology*; D004920:Erythropoiesis; D000860:Hypoxia / Q000503:physiopathology*; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism
chemical_list: D004163:Diphosphoglyceric Acids; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D002256:Carbonic Anhydrases; D001731:Bisphosphoglycerate Mutase