Effect of gamma-thio-ATP on dog stomach smooth muscle membranes.
The effect of gamma-Thio-ATP on high affinity ATPase and on the ATP-dependent Ca-uptake of a plasma membrane fraction prepared from the dog stomach smooth muscle was examined. gamma-Thio-ATP did not act as a substrate for the ATP-dependent Ca-uptake and was only a poor substrate for the high affinity Ca-ATPase reaction. The compound, however, reversibly inhibited the uptake and the ATPase.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002462:Cell Membrane / Q000187:drug effects; D004285:Dogs; D066298:In Vitro Techniques; D009130:Muscle, Smooth / Q000187:drug effects*; D013270:Stomach / Q000187:drug effects; D013873:Thionucleotides / Q000494:pharmacology*
D013873:Thionucleotides; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D000252:Calcium-Transporting ATPases; D002118:Calcium