A high level of transferrin mRNA in the liver of analbuminemic rats.
By means of immunological screening, a cDNA clone bearing the mRNA sequence for rat transferrin was isolated from a cDNA library of rat liver mRNA. The amounts of transferrin mRNA in livers of analbuminemic rats (NAR, Nagase analbuminemia rats) and normal rats were determined by RNA blot hybridization using a cloned transferrin cDNA probe. The level of transferrin mRNA in the NAR liver was about 1.7 times that in the normal rat liver. These findings suggest that the enhanced synthesis of transferrin in the NAR liver resulted from an increase in the transferrin mRNA level.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001798:Blood Proteins / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D006207:Half-Life; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid; D012709:Serum Albumin / Q000172:deficiency*; D014168:Transferrin / Q000235:genetics* / Q000378:metabolism
D001798:Blood Proteins; D004274:DNA, Recombinant; D012333:RNA, Messenger; D012709:Serum Albumin; D014168:Transferrin; D004247:DNA