The basis for major histocompatibility complex (MHC) and immunoglobulin gene control of helper T cell idiotopes.
BALB/c helper T cells, prepared against 2,4,6-trinitrophenyl (TNP)-derivatized syngeneic spleen cells, fail to recognize other hapten modifications [(4-hydroxy-3-nitrophenyl)acetyl, fluorescein isothiocyanate] of syngeneic cells, as well as TNP-derivatized cells from major histocompatibility complex-congenic donors. T helper cell interactions with "presenting" cells and "target" B lymphocytes are inhibited by monoclonal antibodies directed either to the hapten or to I-A molecules on target cells. Helper activity is also inhibited by monoclonal anti-idiotypic antibodies directed to an idiotope expressed by the TNP-binding myeloma protein MOPC460, but not by soluble TNP-protein conjugates. The study of congenic mouse strains revealed that while the fine specificity of TNP recognition and the quantitative levels of M 460 idiotope expression are I-A linked, the expression of the M 460 idiotope by helper cells is controlled by IgCh-linked genes. Absence of anti-idiotope inhibition of helper cells prepared in anti-mu-suppressed mice suggests, however, that immunoglobulin idiotype expression by T cells results from network interactions selecting available lymphocyte repertoires which operate before antigenic stimulation.
D000818:Animals; D005802:Genes, MHC Class II; D006241:Haptens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D013154:Spleen / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D006241:Haptens; D000949:Histocompatibility Antigens Class II; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D014301:Trinitrobenzenes