[Determination of S-IgA and free secretory component in saliva].
Saliva of 95 patients of different ages was investigated for S-IgA and free secretory component (SC). The two-directional rocket method was used as assay for separating S-IgA and free SC in the presence of heparin-Ca-EDTA. The method employs electrophoresis in an antibody containing agarose/agar gel.--The value of S-IgA in children about 4 years old was 40,0 mg/l and the relation of free SC to S-IgA was 1 to 5. The S-IgA level in patients over 50 years old was 55,2 mg/l, in no case free SC was detectable.
D000367:Age Factors; D002118:Calcium / Q000494:pharmacology; D002675:Child, Preschool; D003731:Dental Caries / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007122:Immunoelectrophoresis / Q000379:methods; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000378:metabolism; D007128:Immunoglobulin Fragments / Q000032:analysis*; D008875:Middle Aged; D012463:Saliva / Q000276:immunology*; D012635:Secretory Component / Q000032:analysis*
D007071:Immunoglobulin A, Secretory; D007128:Immunoglobulin Fragments; D012635:Secretory Component; D006493:Heparin; D004492:Edetic Acid; D002118:Calcium
Zum Nachweis von S-IgA und freier sekretorischer Komponente im Speichel.