Endothelialization of a new Dacron graft in an experimental model: light microscopy, electron microscopy and immunocytochemistry.
Two types of synthetic vascular grafts, Dacron Triaxial and Dacron Gelseal Triaxial, were implanted into both the common carotids of sheep. The animals were sacrificed 1, 2, 8, and 16 weeks after surgery. Multiple specimens, obtained from grafts and anastomoses, were studied by light microscopy, transmission and scanning electron microscopy. A parallel immunocytochemical analysis was performed on some specimens. Dacron Triaxial grafts failed to develop a complete neointimal coverage. Myofibroblasts and fibroblasts were the dominant cells in such synthetic graft. Moreover, focal areas of stripping, platelet deposition, and thrombosis were observed at 8 and 16 weeks. In contrast, a stable endothelial coverage developed on the Gelseal Triaxial grafts after 16 weeks.
D000818:Animals; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries / Q000166:cytology / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000166:cytology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011093:Polyethylene Terephthalates; D012756:Sheep; D013997:Time Factors
D011093:Polyethylene Terephthalates