title: Immunoblotting: an emerging technique in immunohematology.
abstract: Protein blotting is a technique of transferring and immobilizing the original protein patterns from gel matrices to a suitable solid phase. It can be employed in identifying the protein antigens in complex biological mixtures, since each protein is accessible to its corresponding antibody. This method, called immunoblotting, is also applicable to define the specificity of a given antibody. The perspective of immunoblotting has already found wide application in basic research. Some features of the immunoblotting and recent improvements of the original procedures will be discussed. To justify a wider application in clinical research I will summarize some applications with respect to immunohematology.
mesh_terms: D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis; D001792:Blood Platelets / Q000032:analysis; D001797:Blood Protein Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007518:Isoantibodies / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D010980:Platelet Membrane Glycoproteins; D012204:Rh-Hr Blood-Group System
chemical_list: D000954:Antigens, Surface; D001323:Autoantibodies; D006023:Glycoproteins; D007518:Isoantibodies; D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins; D012204:Rh-Hr Blood-Group System