Changes in serotonin concentration in a living neurone: a study by on-line intracellular voltammetry.
Intraneuronal concentration of serotonin (5-HT) and its changes were measured in live serotonergic metacerebral cells of Aplysia for several hours following neuronal stimulation, after intracellular injection of 5-HT or extracellular application of L-tryptophan, reserpine, or p-chlorophenylalanine. This was achieved by an on-line intracellular differential pulse voltammetric method using a new, needle-tipped and glass-insulated, platinum microelectrode sensitive to 5-HT.
D001048:Aplysia; D004558:Electric Stimulation; D004563:Electrochemistry / Q000379:methods; D010134:Fenclonine / Q000494:pharmacology; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D012110:Reserpine / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism* / Q000502:physiology; D013237:Stereoisomerism; D014364:Tryptophan / Q000378:metabolism / Q000494:pharmacology
D012701:Serotonin; D012110:Reserpine; D014364:Tryptophan; D010134:Fenclonine