title: Sequence-specific modification of DNA by 6-hydroxybenzo[a]pyrene.
abstract: 6-Hydroxybenzo[a]pyrene cleaved phi X174 supercoiled DNA to open circular DNA in the presence of heavy metal ions. It induced an alkali-labile modification in DNA via an oxygen-radical-mediated reaction; the most frequent alkali-labile sites were on the 3' side of the pyrimidine residues of the pyrimidine cluster.
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics; D001483:Base Sequence; D001580:Benzopyrenes / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism; D004247:DNA / Q000187:drug effects*; D004278:DNA, Superhelical / Q000187:drug effects; D004279:DNA, Viral / Q000187:drug effects
chemical_list: D001580:Benzopyrenes; D004278:DNA, Superhelical; D004279:DNA, Viral; D003300:Copper; D004247:DNA; C023395:6-hydroxybenzo(a)pyrene