Mononuclear phagocytes as targets for cytolytic T lymphocytes.
Mouse peritoneal macrophages and human blood monocytes are excellent targets for the assay of cytolytic T lymphocytes that develop in the murine and human mixed leukocyte reactions, respectively. 51Cr release in a standard 4 h assay is equivalent to the best release that is obtained with conA-induced T lymphoblasts. Spontaneous release is consistently less than 20% of the total cell label, and syngeneic macrophages are not lysed. Unlike lymphoblasts, mononuclear phagocytes can be used either as freshly isolated cells or after prolonged periods (1-7 days) in culture.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000276:immunology; D010586:Phagocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*