Use of monoclonal antibodies to separate the enantiomers of abscisic acid.
The resolution of racemates often requires difficult and time consuming purification procedures. McAb technology allows the production of specific antibodies in quantities suitable for the preparation of matrices for large scale affinity purification. Here we report the rapid separation of abscisic acid (ABA) enantiomers by affinity chromatography using McAb. This method appears to be far superior to previously published separations based on crystallization, chromatography, and affinity purification with conventional antisera. The approach here described will be particularly attractive in a wide variety of similar situations.
D000040:Abscisic Acid / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D003509:Cyclohexanecarboxylic Acids / Q000302:isolation & purification*; D007120:Immunochemistry; D013237:Stereoisomerism
D000911:Antibodies, Monoclonal; D003509:Cyclohexanecarboxylic Acids; D000040:Abscisic Acid