Desmutagenic effect of humic acid.
In the present study, humic acid was not found to be mutagenic and did not inhibit spontaneous mutation. It did, however, inhibit the mutagenicities of benzo[a]pyrene and 3-aminoanthracene (+S9 mix), but not the mutagenicities of 4NQO, AF-2 and MNNG (-S9 mix). 2-Nitrofluorene and 1-nitropyrene do not need S9 mix for activation of their mutagenicities, but an inhibitory effect was observed. Humic acid exerts a desmutagenic effect on mutagens directly before they act on cells. It does not act as an antimutagen which blocks the processes changing normal cells to mutants. The desmutagenic effect was not decreased by heat treatment (120 degrees C, 15 min). Humic acid was fractionated according to molecular weight and the desmutagenic effect increased with an increase in molecular weight. This effect in the fraction with molecular weight exceeding 300 000 was decreased by centrifugation. The desmutagenic ability of humic acid may result from soluble components and adsorption to small particles.
D000818:Animals; D006358:Hot Temperature; D006812:Humic Substances / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000378:metabolism; D008970:Molecular Weight; D009152:Mutagenicity Tests; D009153:Mutagens / Q000037:antagonists & inhibitors*; D009154:Mutation / Q000187:drug effects*; D051381:Rats
D006812:Humic Substances; D009153:Mutagens