A radioenzymatic assay for quinolinic acid.
A new and rapid method for the determination of the excitotoxic tryptophan metabolite quinolinic acid is based on its enzymatic conversion to nicotinic acid mononucleotide and, in a second step utilizing [3H]ATP, further to [3H] deamido-NAD. Specificity of the assay is assured by using a highly purified preparation of the specific quinolinic acid-catabolizing enzyme, quinolinic acid phosphoribosyltransferase, in the initial step. The limit of sensitivity was found to be 2.5 pmol of quinolinic acid, sufficient to conveniently determine quinolinic acid levels in small volumes of human urine and blood plasma.
D000328:Adult; D006801:Humans; D008297:Male; D009243:NAD / Q000031:analogs & derivatives; D009537:Nicotinamide Mononucleotide / Q000031:analogs & derivatives; D010430:Pentosyltransferases; D011725:Pyridines / Q000032:analysis*; D017378:Quinolinic Acid; D011805:Quinolinic Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D014316:Tritium; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
D011725:Pyridines; D011805:Quinolinic Acids; D009243:NAD; D014316:Tritium; D009537:Nicotinamide Mononucleotide; C002953:nicotinate mononucleotide; C018348:nicotinic acid adenine dinucleotide; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating); D017378:Quinolinic Acid