Binding of (+)PHNO and other D2-dopamine agonists to D1-dopamine receptors labelled by [3H]SCH 23390.
The affinity of (+)PHNO for the D1-dopamine receptor subtype in striatal tissue was determined in a radioligand binding assay using the D1-selective compound, [3H]SCH 23390. The IC50 for (+)PHNO was 22 microM, a value considerably higher than obtained for reference agents (lisuride, apomorphine, pergolide, [-] EOE and bromocriptine). The low potency of (+)PHNO at the D1-receptor subtype contrasts with the compound's high potency at the D2-receptor subtype, as reported previously. (+)PHNO can, therefore, be classified as a very selective D2-receptor agonist.
D000818:Animals; D001552:Benzazepines / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D008297:Male; D010078:Oxazines / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000378:metabolism*; D017447:Receptors, Dopamine D1
D001552:Benzazepines; D010078:Oxazines; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; C042631:naxagolide