Abundance of the Ca2+-pumping ATPase in pig erythrocyte membranes.
The Ca2+-pumping ATPase (Ca2+-ATPase) was purified from human and pig erythrocyte membranes by calmodulin affinity chromatography in the presence of phosphatidylcholine. The amount of enzyme present in pig erythrocytes is at least 7 times greater than that isolated from human erythrocyte ghosts. However, the properties of the enzyme from the two species are similar in many respects.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000097:blood* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D013552:Swine; D014638:Vanadates / Q000494:pharmacology
D008081:Liposomes; D014638:Vanadates; D000252:Calcium-Transporting ATPases