[A new ELISA method for the diagnosis of toxoplasmosis. Assay of serum IgM by immunocapture with an anti-Toxoplasma gondii monoclonal antibody].
The diagnosis of acute toxoplasmosis is based exclusively on the detection of IgM anti-Toxoplasma antibodies. The principal of the immunoenzymatic test reported here is the capture of serum IgM antibodies which are detected indirectly by the sequential addition of antigen and a monoclonal antibody directed against the immunodominant epitope at the surface of the tachyzoite. This test combines the sensitivity of enzyme-based assays and the specificity of monoclonal reagents and represents an important contribution to the diagnosis of Toxoplasma gondii infection.
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M
Une nouvelle méthode ELISA pour le diagnostic de la toxoplasmose. Dosage des IgM sériques par immunocapture avec un anticorps monoclonal anti-Toxoplasma gondii.