Chromate reduction by rabbit liver aldehyde oxidase.
Chromate was reduced during the oxidation of 1-methylnicotinamide chloride by partially purified rabbit liver aldehyde oxidase. In addition to 1-methylnicotinamide, several other electron donor substrates for aldehyde oxidase were able to support the enzymatic chromate reduction. The reduction required the presence of both enzyme and the electron donor substrate. The rate of the chromate reduction was retarded by inhibitors of aldehyde oxidase but was not affected by substrates or inhibitors of xanthine oxidase. These results are consistent with the involvement of aldehyde oxidase in the reduction of chromate by rabbit liver cytosolic enzyme preparations.
D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D002840:Chromates / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009536:Niacinamide / Q000031:analogs & derivatives / Q000378:metabolism; D010084:Oxidation-Reduction; D011190:Potassium Cyanide / Q000494:pharmacology; D011817:Rabbits; D014812:Vitamin K / Q000494:pharmacology
D002840:Chromates; D014812:Vitamin K; D009536:Niacinamide; D000493:Allopurinol; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase; D011190:Potassium Cyanide; C024058:N(1)-methylnicotinamide