Isolation of a cDNA for human muscle 6-phosphofructokinase.
A cDNA for human muscle 6-phosphofructokinase (EC.2.7.1.11) has been isolated from a human fibroblast cDNA library made using the Okayama-Berg procedure. The cDNA isolated as a Bam H1 fragment of the pcD recombinant, pO4, is approximately 2000 bp in length. It represents approximately 1350 bp of the C-terminus coding sequence of the enzyme, approximately 500 bp of the 3'-untranslated region and approximately 150 bp of the vector sequences. The identity of the pO4 cDNA was established by the observation of a high degree of homology (approximately 95%) between the deduced amino acid sequence with the published protein sequence of rabbit muscle 6-phosphofructokinase, and the assignment of the sequence to human chromosome 1 (the known location of PFKM) by using somatic cell hybrids. Based on immunochemical evidence, we had previously predicted not only a remarkable structural conservation of the vertebrate muscle PFK, but also partial structural identity among all three vertebrate PFK isozymes. The pO4 cDNA is, therefore, expected to permit isolation of cDNAs for muscle and non-muscle PFKs from a wide variety of vertebrate species.
D000818:Animals; D001345:Autoradiography; D002874:Chromosome Mapping; D004247:DNA / Q000302:isolation & purification*; D005347:Fibroblasts; D006801:Humans; D009132:Muscles / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D010732:Phosphofructokinase-1 / Q000235:genetics*; D010957:Plasmids; D011817:Rabbits; D013045:Species Specificity
D004247:DNA; D010732:Phosphofructokinase-1