Characterization of immunoglobulin G-Fc receptor activity in the outflow system of the cerebrospinal fluid.
We have examined IgG Fc receptor (FcR) activity of human and rabbit arachnoid granulations and leptomeninges using antibody (IgG)-coated erythrocytes (EIgG), covalently crosslinked IgG dimers, trimers and oligomers, immune complexes, aggregated Fc fragments and a monoclonal anti-human neutrophil Fc receptor antibody, 3G8. EIgG bound specifically to cells of the leptomeninges and arachnoid granulations; uncoated erythrocytes, F(ab')2-coated, or IgM-coated erythrocytes failed to bind. The specificity of this interaction was demonstrated by inhibition studies. Monomeric IgG and Fc fragments blocked EIgG adherence, whereas bovine serum albumin (BSA), Fab fragments of IgG and the monoclonal anti-neutrophil FcR antibody failed to inhibit EIgG adherence. Monomeric IgG inhibited FcR function in a dose-dependent fashion; maximal inhibition was achieved at 1.7 X 10(-5)M IgG, indicating a relatively low avidity receptor. Oligomers of IgG inhibited EIgG adherence more effectively and inhibition was directly related to oligomer size. Additionally, these tissues were positive for specific and non-specific esterases. These studies suggest that the CSF pathway from the perivascular spaces to the arachnoid granulations plays a protective role in the clearance of IgG and IgG immune complexes in infections and immune-mediated disorders.
D000818:Animals; D001344:Autopsy; D001921:Brain / Q000276:immunology* / Q000473:pathology; D005123:Eye / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000134:cerebrospinal fluid; D008577:Meningeal Neoplasms / Q000276:immunology; D008579:Meningioma / Q000276:immunology; D011817:Rabbits; D011961:Receptors, Fc / Q000032:analysis*; D017452:Receptors, IgG; D013154:Spleen / Q000276:immunology / Q000473:pathology
D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG