Evidence for the expression of protein IX in some rat cells transformed with adenovirus type 12 early region 1 DNA.
Sera were produced by injection of a particular rat cell line (HLBRK EcoC1), produced by transformation of baby rat kidney cells with adenovirus 12 early region 1 DNA, into the syngeneic host. Antibodies to a protein of Mr 15,200 as determined by SDS-PAGE, were detected in the sera from two rats bearing tumours. The 15K protein was identified as the viral structural component polypeptide IX by a number of criteria: (i) the protein was only observed in adenovirus 12-infected cells, (ii) it was produced at intermediate and late times after infection, (iii) it was immunoprecipitated from purified adenovirus 12 particles and (iv) it was immunoprecipitated from a purified protein preparation.
D000256:Adenoviridae / Q000235:genetics*; D017456:Adenovirus Early Proteins; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D007668:Kidney; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats
D017456:Adenovirus Early Proteins; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral