Identification of two macrophage populations by flow cytometry monitoring of oxidative burst and phagocytic functions.
Two populations of phagocytic cells from trehalose dimycolate-elicited mouse peritoneal cells are demonstrated by flow cytofluorometry, using two fluorescent probes excited at the same wavelength (488 nm). Liposomes containing diethylenetriaminepentaacetate daunorubicin conjugate (maximum emission wavelength: 590 nm) allow the discrimination of phagocytes and non-phagocytic cells. Among the phagocytes, an activated population is revealed by a cell-associated fluorescence of the oxidation product of dichlorofluorescein diacetate (maximum emission wavelength: 520 nm).
D000818:Animals; D005434:Flow Cytometry / Q000379:methods; D008081:Liposomes; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D010101:Oxygen Consumption; D010587:Phagocytosis; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D008081:Liposomes; D013755:Tetradecanoylphorbol Acetate