Identification of alpha atrial natriuretic peptide [4-28] and [5-28] in porcine brain.
Although atrial natriuretic peptide (ANP) has recently been verified to function as a neuropeptide in the central nervous system, its definite identification has not been done so far. We have isolated two ANP-related peptides from porcine brain by utilizing alpha-ANP specific radiommunoassay coupled with immunoaffinity chromatography and reverse phase HPLC. By structural analyses, these two peptides were determined to be alpha-ANP [4-28] and alpha-ANP [5-28]. They were found to elicit chick rectum relaxant activity comparable to alpha-ANP. These results indicate that proteolytic processing of ANP precursor in the central nervous system takes place in a manner different from that in heart and plasma, where gamma-ANP and alpha-ANP are known to be the main components of ANP, respectively.
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009126:Muscle Relaxation / Q000187:drug effects; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000378:metabolism*
D010446:Peptide Fragments; C081681:atrial natriuretic factor (4-28); D009320:Atrial Natriuretic Factor; C054538:atrial natriuretic factor (5-28)