Differential human interferon alpha receptor expression on proliferating and non-proliferating cells.
The expression of interferon-alpha (IFN-alpha) receptors was studied on a variety of human cells, using monoiodinated IFN-alpha 2 probes. Steady-state binding at 4 degrees C revealed a single class of non-interacting IFN receptor on peripheral blood lymphocytes, and tonsillar B lymphocytes, which are both known to be G0/G1 resting cell populations. The binding affinity of this class of receptor was found to be on the order of 5 X 10(-10) M, expressed as an apparent dissociation constant (Kd). However, cells proliferating either in culture or in vivo were found to express a heterogeneity in IFN-alpha 2 binding. Such binding could be objectively resolved (by a version of the LIGAND program of P. Munson) into a two-site receptor model. Hill plots of binding to proliferating cells indicated a negative cooperativity in the interaction of IFN and receptor. The high-affinity component, expressed on proliferating cells, typically exhibits a Kd of (1-10) X 10(-11) M, while the lower-affinity component indicates a Kd of (1-10) X 10(-9) M. Furthermore, the low-affinity component is apparently expressed on the order of 10-200 times the copy number, per cell, of the high-affinity site. Affinity-labeling experiments revealed that, in addition to the 140-160-kDa IFN-binding complex reported by others, both the proliferating and non-proliferating cell populations possess a novel IFN-binding component of 60 kDa.
D000345:Affinity Labels / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology; D002455:Cell Division; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism; D007399:Interphase; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism; D008970:Molecular Weight; D014066:Palatine Tonsil / Q000166:cytology; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D017471:Receptors, Interferon; D012984:Software
D000345:Affinity Labels; D007370:Interferon Type I; D011971:Receptors, Immunologic; D017471:Receptors, Interferon