Modulating effects of drugs on cell cycle kinetics.
The effects of individual drugs on cell cycle progression can often be determined by analyzing the DNA distribution of cultured cells at appropriate times after drug administration. In addition, to cell counts, RNA and/or protein content, the alterations in cell cycle distribution of drug-treated cells can yield information on the potential cell cycle phase specificity of the drug. However single parameter DNA analysis, as currently used, can give inaccurate information when drug effects are associated with cytokinesis perturbations, in spite of various statistical and mathematical analyses. Scanning flow cytometry could be an interesting alternative for the detection of binucleate cells.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D007700:Kinetics; D010396:Penicillamine / Q000494:pharmacology*; D011817:Rabbits
D002087:Butyrates; D020148:Butyric Acid; D004247:DNA; D010396:Penicillamine