Suppression of cell proliferation after incubation of allogeneically stimulated mouse lymphocytes with concanavalin A: serological analysis of cell subpopulations involved.
The effect of Con A incubation of allogeneically stimulated lymphocytes (a-MLC) on cell proliferation was tested in three experimental systems: in the a-MLC cell population itself; after transfer of a-MLC cells to a culture of normal spleen cells with Con A; and after transfer of supernatants from cultures of a-MLC cells with Con A to fresh cultures of normal spleen cells with Con A. It was found that incubation with Con A to fresh cultures of normal spleen cells with Con A. It was found that incubation with Con A caused inhibition of cell proliferation in all three systems, indicating the generation of suppressor cells by incubation of a-MLC cells with Con A. The suppressive activity was abolished by cytotoxic treatment with either anti-Thy-1 or anti-Lyt-1 monoclonal antibodies, but neither with anti-L3T4 nor with anti-Lyt-2 antibodies, in all three systems.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000911:Antibodies, Monoclonal; D003208:Concanavalin A