Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry.
Flow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of the whole cell and its organelles. Rhodamine 123 is a vital fluorescent dye used in flow cytometry. As it is specifically concentrated in mitochondria because of the transmembrane potential that these organelles maintain in living cells, rhodamine 123 is thus a useful probe for monitoring the abundance and activity of mitochondria. A critical survey of the routine use of rhodamine 123 together with flow cytometry in mitochondrial research is presented.
D000818:Animals; D004396:Coloring Agents; D005434:Flow Cytometry / Q000379:methods; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D020112:Rhodamine 123; D012235:Rhodamines / Q000378:metabolism; D014966:Xanthenes / Q000378:metabolism
D004396:Coloring Agents; D012235:Rhodamines; D014966:Xanthenes; D020112:Rhodamine 123