Inhibition of rat mixed lymphocyte pancreatic islet cultures with anti-Ia immunotoxin.
Studies reported here indicate that an anti-Ia immunotoxin can eliminate the allostimulatory subpopulation of cells present within the islets of Langerhans without damaging the hormone-secreting cells. Such studies made use of an in vitro correlate of transplantation rejection, the mixed lymphocyte islet cell (MLIC) reaction. Using the MLIC, it was demonstrated that an anti-Ia immunotoxin removed cells capable of stimulating the MLIC in a dose-dependent fashion without altering the hormone-secreting functions of the remaining cells when challenged with glucose and theophylline. These studies suggest the feasibility of using such anti-Ia immunotoxins in islet allograft transplantation models to circumvent problems inherent in complement-mediated cytotoxicity, a previously documented effective form of inducing islet allotransplantation tolerance.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D000922:Immunotoxins / Q000008:administration & dosage*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051381:Rats; D012276:Ricin / Q000008:administration & dosage
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D000922:Immunotoxins; D007328:Insulin; D012276:Ricin