Factors which equalize the representation of genome segments in recombinant libraries.
Genomic segments which contain inverted repetitions longer than 300 bp are frequently lost from recombinant libraries grown on rec+ hosts. We have found that 9% of phage lambda clones that contain 15-20-kb insertions of human or Drosophila DNA are inhibited on rec+ hosts and as a result will become under-represented in amplified genomic libraries. We have therefore examined several factors of both host and vector origin which affect the fidelity of representation of genomic sequences in recombinant DNA libraries constructed in bacteriophage lambda vectors. This loss may be diminished if the vector carries either a chi element or a functional gam gene. The most successful approach, however, involves using a host with mutations in recB, recC, and sbcB, or in recD. We have shown that recombinant clones which require such mutant hosts for growth are somewhat more likely to contain DNA derived from loci in the genome which are polymorphic than are clones recovered on conventional hosts.
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000502:physiology; D005822:Genetic Vectors; D006801:Humans; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D014764:Viral Proteins; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli