Prevention of graft-versus-host disease in allogeneic bone marrow transplantation by pretreatment with 2'-deoxycoformycin.
Germ-free mice were used as a model for acute graft-versus-host disease (GVHD) in allogeneic bone marrow transplantation (BMT). C3H/He recipients of DBA/2 cells showed typical symptoms of acute GVHD and died within 8 days. Incubation of the cells with 10 microM 2'-deoxycoformycin (2dCF) + 100 microM deoxyadenosine (dAdo) for 1 h inhibited all T-cell functions as well as T-cell-dependent B-cell functions, but had no effect on B-cell functions that are T-cell independent, nor on the hemopoietic stem cells (CFU-S). Recipients of allogeneic cells that had been incubated with 2dCF + dAdo for 1 h prior to inoculation showed no signs, gross or histological, of acute or chronic GVHD up to 15 months after transplantation. The recovery patterns of the blood and bone marrow were not affected by the treatment, and were similar to those of recipients of treated and untreated syngeneic cells.
D000818:Animals; D016026:Bone Marrow Transplantation; D002678:Chimera; D003070:Coformycin / Q000031:analogs & derivatives / Q000627:therapeutic use*; D003839:Deoxyadenosines / Q000494:pharmacology; D006086:Graft vs Host Disease / Q000517:prevention & control*; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D015649:Pentostatin; D011292:Premedication; D012263:Ribonucleosides / Q000627:therapeutic use*; D013601:T-Lymphocytes / Q000187:drug effects; D014184:Transplantation, Homologous
D003839:Deoxyadenosines; D012263:Ribonucleosides; D003070:Coformycin; D015649:Pentostatin