Impaired humoral immune response in complement C3-deficient guinea pigs: absence of secondary antibody response.
A recently described genetically controlled C3 deficiency (C3D) in guinea pigs (GP) provided a unique model for studying the role of C3 in the afferent limb of the humoral immune response in a direct manner. These C3D animals, which have only 5-7% of normal serum C3 level, were immunized with the bacteriophage phi chi 174, a T cell-dependent antigen, followed by a booster injection after 4 weeks (1.5 X 10(9) plaque-forming units/kg). The formation of IgM and IgG antibody in the course of the primary and secondary response was determined and compared with a control group of inbred strain 2 GP. The C3D animals showed a markedly diminished antibody response to this antigen. Amplification of the antibody titer as well as regular isotype switching from IgM to IgG was absent in the secondary response. Increasing the amount of antigen to a high dose (1 X 10(10) plaque-forming units/kg) led to a normalization of the antibody response. The impairment in antibody formation resembles closely the impaired antibody response in C4-deficient or C2-deficient GP, which both have a block in activation of C3 via the classical pathway. However, in contrast to C4D GP or C2D GP the C3D GP do not exhibit serological characteristics of immune complex disease. They have normal levels of total serum IgM, of IgM anti-2,4-dinitrophenyl antibodies and of IgM rheumatoid factors.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D010584:Bacteriophage phi X 174 / Q000276:immunology; D003176:Complement C3 / Q000172:deficiency* / Q000502:physiology; D004136:Dinitrobenzenes / Q000276:immunology; D004546:Elapid Venoms / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male
D003176:Complement C3; D004136:Dinitrobenzenes; D004546:Elapid Venoms; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C016310:cobra venom factor