title: Structure, biosynthesis, and polymorphism of chicken MHC class II (B-L) antigens and associated molecules.
abstract: Chicken MHC class II (B-L) antigens were immunoprecipitated by the monoclonal antibody TaP1 from inbred chicken splenic leukocytes and a lymphoblastoid B cell line (RP9), and were studied by two dimensional gel electrophoresis. B-L antigens are composed of one alpha and one beta chain that are noncovalently bound at the cell surface. In all haplotypes studied, a single acidic 34,000 dalton non-polymorphic chain was observed, whereas two polymorphic chains could be distinguished, differing in both pH and m.w. The alpha-beta heterodimer is associated during its maturation in the cytoplasm with several basic invariant molecules with m.w. ranging from 30,000 to 42,000 daltons. Treatment of cells with tunicamycin and treatment of immunoprecipitated molecules with several glycosidases revealed a complex process of maturation for all of these molecules. The alpha and beta chains undergo a N-glycosylation of complex type, whereas the invariant molecules bear N-linked high mannose glycans, and perhaps also O-linked glycans in the RP9 lymphoblastoid line. Overall, the B-L antigens appear very similar to the HLA-DR and I-E antigens.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D000944:Antigens, Differentiation, B-Lymphocyte; D002236:Carbohydrate Conformation; D002645:Chickens / Q000276:immunology*; D003545:Cysteine / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D007784:Lactoperoxidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008715:Methionine / Q000378:metabolism; D011110:Polymorphism, Genetic; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D014415:Tunicamycin
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; C041136:Ia-like antigens, avian; C045084:invariant chain; D014415:Tunicamycin; D008715:Methionine; D007784:Lactoperoxidase; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D003545:Cysteine