New radioimmunoassay for the detection of free IL2 receptor in supernatants of murine helper and cytotoxic T-cell lines.
Using two purified monoclonal antibodies which recognize distinct epitopes of the murine IL2 receptor, we devised a new radioimmunoassay for the detection of free IL2 receptor in lymphocyte culture supernatants. Murine cell lines of both helper and cytotoxic phenotypes produce free IL2 receptor. There appears to be a quantitative relationship between membrane IL2 receptor expression and the presence of free IL2 receptor. The latter molecule may have a biological role, possibly that of a negative signal for lymphocyte growth and/or that of an IL2 transport protein.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D051379:Mice; D011863:Radioimmunoassay / Q000379:methods*; D011971:Receptors, Immunologic / Q000032:analysis* / Q000276:immunology; D015375:Receptors, Interleukin-2; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2