Growth kinetics of the G2-phase of Ehrlich ascites tumor cells, separated from anaerobically treated asynchronous cultures.
Cell cycle progression of G2 fractions (75-80% G2 (4C) cells) from 8 h anaerobically cultured asynchronous hyperdiploid Ehrlich ascites tumor cells strain Karzel, separated by centrifugal elutriation, was studied after reaeration by flow cytometric methods, including the BrdU-H33258 technique and dual parameter measurements. Analyses of the growth kinetics demonstrated that one fraction of the cell population proceeds through a normal cell cycle (2C----4C) with a generation time of about 20 h. Another portion entered a new cycle (4C----8C) to form cells with a DNA content up to 8C; mono-, bi- and polynucleate cells could be detected. After 15 h aerobic recultivation of the anaerobically cultured G2 cells, a fraction containing 80-85% with a DNA content of greater than 4C was separated. On recultivation, these cells pass a 4C----8C division cycle with a generation time of about 10 h, and a G1 period of less than 4 h.
D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D002453:Cell Cycle; D002455:Cell Division; D005434:Flow Cytometry / Q000379:methods; D007399:Interphase; D007700:Kinetics; D051379:Mice; D011123:Polyploidy