[Effect of tetracaine and sovcaine on the ATP-dependent calcium accumulation in sarcoplasmic reticulum vesicles of skeletal muscles].
The tetracaine and cinchocaine in concentration less than 2 mM and 0.5 mM, respectively, stimulate ATP-dependent Ca-loading by enhancing the initial rate of Ca2+-accumulation, do not affect the Ca2+-binding and Ca-ATPase activity of sarcoplasmic reticulum vesicles. These data suggest blocking of Ca2+-efflux from vesicles which occurs during Ca-accumulation. Higher concentrations of the same compounds (above 2 mM and 0.5 mM for tetracaine and cinchocaine, respectively) caused inhibition of the Ca-ATPase activity and decreased the ability of SR vesicles to retain Ca2+, probably, due to their nonspecific lipophilic action.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D003992:Dibucaine / Q000494:pharmacology*; D066298:In Vitro Techniques; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013268:Stimulation, Chemical; D013748:Tetracaine / Q000494:pharmacology*
D013748:Tetracaine; D002110:Caffeine; D000255:Adenosine Triphosphate; D000252:Calcium-Transporting ATPases; D003992:Dibucaine; D002118:Calcium
Vliianie tetrakaina i sovkaina na ATP-zavisimoe nakoplenie kal'tsiia vezikulami sarkoplazmaticheskogo retikuluma skeletnykh myshts.