Fc gamma receptor effects on induction of c-myc mRNA expression in mouse B lymphocytes by anti-immunoglobulin.
Levels of c-myc mRNA have been assayed in mouse B cells cultured for 8 hr with Fab'2 anti-Ig or IgG anti-Ig. Fab'2 anti-Ig induces DNA synthesis in B cells, whereas the whole molecule inhibits anti-Ig-induced DNA synthesis by crosslinking the B cell Fc gamma R to mIg. Both the Fab'2 fragment and the IgG anti-Ig induce an increase in c-myc mRNA by 1 hr. Thereafter, levels in cells stimulated with submitogenic doses of Fab'2 anti-Ig or any dose of IgG anti-Ig returned to background, while levels in cultures containing a mitogenic dose of Fab'2 anti-Ig remained elevated.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008811:Mice, Inbred DBA; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis*; D011961:Receptors, Fc / Q000276:immunology*; D017452:Receptors, IgG
D000888:Antibodies, Anti-Idiotypic; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011961:Receptors, Fc; D017452:Receptors, IgG