[Electrophoresis of DNA fragments renatured and cross-linked with psoralen: kinetic analysis of DNA photocross-linking].
The kinetics of photoreactions between DNA and psoralen was analysed. A method is described for determining the dependence of the average number of cross-links per base-pair (n) on the fluence of UV-irradiation. This calibration curve allows to specify the conditions of irradiation necessary to obtain any desired value of n.
D010582:Bacteriophage lambda; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects; D004279:DNA, Viral / Q000378:metabolism / Q000528:radiation effects; D004587:Electrophoresis, Agar Gel; D005363:Ficusin / Q000378:metabolism*; D011564:Furocoumarins / Q000378:metabolism*; D007700:Kinetics; D009695:Nucleic Acid Renaturation; D010777:Photochemistry; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D004279:DNA, Viral; D011564:Furocoumarins; D004247:DNA; D005363:Ficusin
Elektroforez sshitykh psoralenom i renaturirovannykh fragmentov DNK: kineticheskiĭ analiz fotosshivaniia DNK.