Expression of human T antigens in interspecies hybridomas.
Interspecies hybrids were constructed by fusing normal human male peripheral blood mononuclear cells with BW5147, a HGPRT- thymoma derived from an AKR mouse. Hybrid cells were selected in HAT media in culture dishes containing 1 X 10(7) human red blood cells. Twelve weeks after fusion, hybridomas were diluted to 10-15 cells/well and characterized for their expression of the human immune cell surface antigens HLA-DR, T3, T4, and T8 using fluorescent microscopy and cytographic analysis. More than 70% of the hybrid colonies expressed human T-cell surface antigens. Moreover the specific human repetitive DNA (ALU) bound to DNA sequences isolated from the hybridomas after Southern transfers. However, the same hybrids did not have a statistically significant increase in their chromosome number when compared to the mouse parent cell line. Several of the hybridomas produced a soluble factor capable of stimulating the growth of the IL-2 restricted murine cell line CTLL-2 and supported DNA synthesis in human peripheral T-cell populations. Panning experiments demonstrated that the IL-2 producing hybridomas could be enriched by selecting for the human T-cell surface antigen T3. The results presented here indicate that mouse X human hybridomas which express a broad range of human lymphocyte markers can be constructed and maintained in continuous culture for extended periods of time. It also appears that the T3-Ti receptor complex mediates the proliferation of T cells through the T3 molecules linkage to the secretion and/or production of IL-2. The usefulness of interspecific T-cell hybrids as an immunogenetic research tool as well as the significance of the mapping data are discussed.
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D007621:Karyotyping; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D003470:Culture Media; D007376:Interleukin-2