Functional assay of plasminogen activator by hydrolysis of 14C-globin.
A functional method for estimation of plasminogen activator (PA) activity is described. The end-point method follows the PA-dependent conversion of the inactive plasminogen to the potent protease plasmin. The formation of plasmin is monitored by its activity on 14C-globin to form acid-soluble radioactive products. The globinolytic assay of PA shows sensitivity similar to that of the 125I-fibrinolytic assay, and is superior in sensitivity to both the chromogenic (Pyro-Glu-Gly-Arg-pNA, S-2444) and the fluorimetric (Glu-Gly-Arg-methylcoumarin amide) methods. The assay is applicable for estimation of tissue type and urokinaselike PA activity in biologic samples, including viable cells in suspension and conditioned medium.
D002460:Cell Line; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis; D005914:Globins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology; D010960:Plasminogen Activators / Q000032:analysis* / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D005914:Globins; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator