title: Effects of heparan sulfate removal on attachment and reattachment of fibroblasts and endothelial cells.
abstract: Human skin fibroblasts and calf aorta endothelial cells were grown as tissue culture monolayers in the presence of [35S]sulfate in order to label the glycosaminoglycan portions of proteoglycans for investigation of their role in cell attachment. The [35S]glycosaminoglycans were then selectively removed from the cell monolayers by the addition of various glycosaminoglycan-degrading enzymes. As previously described, in contrast to trypsin treatment none of these enzymes removed any cells from the culture plates. Incubation with a preparation from Flavobacterium heparinum left only small stubs of [35S]glycosaminoglycans on the cell monolayers, indicating that all the cell-surface proteoheparan [35S]sulfate and proteochondroitin [35S]sulfate was accessible to this enzyme preparation. The treatment did not change the amount or time of incubation with trypsin necessary for release of the cells from the monolayers. Thus, cell attachment was not weakened by removal of heparan sulfate or chondroitin sulfate. In contrast, neither fibroblasts nor endothelial cells in suspension would reattach in the presence of the F. heparinum preparation while reattachment occurred readily in the presence of chondroitin ABC lyase. This provides evidence that heparan sulfate, but not chondroitin sulfate, is involved in the process of cell attachment even though neither is necessary for maintaining attachment.
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology*; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D004727:Endothelium / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology; D006025:Glycosaminoglycans / Q000502:physiology*; D006497:Heparan Sulfate / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D012879:Skin Physiological Phenomena; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes
chemical_list: D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D013431:Sulfates; D013462:Sulfur Radioisotopes