Molecular forms of atrial natriuretic peptides in rat tissues and plasma.
By using the sensitive radio-immunoassay specific for rat atrial natriuretic peptide (rANP) combined with chromatographic separation, we have determined molecular forms of rANP in rat tissues and plasma. Gamma-rat atrial natriuretic peptide is a major component of rANP in rat atria and ventricles, while alpha-rANP is a predominant form in the plasma, brain and submaxillary gland. Although molecular patterns in the adrenal and thyroid glands were very similar to that in the brain, a major immunoreactive (ir) alpha-rANP peak was accompanied by two minor peaks due to gamma-rANP and another unidentified ir-rANP. This variety in molecular forms suggests that the processing system for rANP precursors differs from tissue to tissue.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D011487:Protein Conformation; D011863:Radioimmunoassay; D051381:Rats; D014018:Tissue Distribution
D009320:Atrial Natriuretic Factor