title: Stimulative effect of glia maturation factor and acetylcholine on active amino acid uptake and Ca2+,Mg2+-ATPase activity in nodose ganglia excised from adult rats.
abstract: During aerobic incubation at 37 degrees C, active uptake of labeled non-metabolizable alpha-aminoisobutyric acid (AIB) into isolated nodose ganglia (NG) excised from adult rats was accelerated to nearly twice that of the control, by the addition of glia maturation factor (GMF, 5 micrograms/ml) in a dose-dependent manner. A similar but moderate stimulative effect on ganglionic AIB uptake was caused by the addition of acetylcholine (ACh, 1 mM) plus eserine (0.1 mM). This effect, however, was not antagonized by nicotinic (hexamethonium, C6, 0.1 mM) or muscarinic (atropine, 0.1 mM) blockers. The GMF-induced amino acid uptake seemed to be inhibited by further addition of ACh. On the other hand, ganglionic Ca2+,Mg2+-ATPase activity was greatly stimulated by either GMF or ACh. These results suggest that the increase in AIB uptake induced by GMF or ACh is possibly linked to Ca2+,Mg2+-ATPase activity in NG cell membranes.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D005260:Female; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000378:metabolism; D020904:Glia Maturation Factor; D008297:Male; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009620:Nodose Ganglion / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014630:Vagus Nerve / Q000187:drug effects*
chemical_list: D000596:Amino Acids; D000621:Aminoisobutyric Acids; D020904:Glia Maturation Factor; D009419:Nerve Tissue Proteins; C017911:2-(methylamino)isobutyric acid; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D000109:Acetylcholine