Determination of chinoform in biological fluids and nervous tissues of the dog by gas chromatography-mass spectrometry.
A sensitive gas chromatographic-mass spectrometric method for the determination of 5-chloro-7-iodo-8-hydroxyquinoline (chinoform, clioquinol) in biological fluids and nervous tissues is described. Chinoform was converted into the pentafluorobenzyl ether, which was separated on a 10% Dexsil 300GC column and determined by the use of chinoform-d4 as an internal standard. The clean-up of chionoform in plasma and urine was efficiently achieved by extracting with benzene, while the drug in the tissue was pretreated successively by extraction with 12.5% v/v pyridine-benzene, separation on a Clin-Elut cartridge and adsorption on alumina. The quantitation limit of chinoform was 100 pg, and the recovery rates of chinoform added to plasma and tissue were 98% and 92%, respectively. The chinoform levels in biological fluids and tissues in dogs after prolonged administration of the drug at a dose of 400 mg/kg/day were measured by the proposed method. The plasma level and tissue distribution of chinoform are also discussed.
D000818:Animals; D002849:Chromatography, Gas; D007464:Clioquinol / Q000032:analysis* / Q000097:blood / Q000652:urine; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry; D006912:Hydroxyquinolines / Q000032:analysis*; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000032:analysis; D009417:Nerve Tissue / Q000032:analysis*
D006912:Hydroxyquinolines; D007202:Indicators and Reagents; D007464:Clioquinol