title: The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites.
abstract: The Fos protein complex and several Fos-related antigens bind directly or indirectly to a common sequence element that is similar to the consensus binding site for HeLa cell activator protein 1 (AP-1). This element is present in a negative regulatory sequence in the differentiation-sensitive adipocyte gene, aP2; in a transcriptional enhancer for the Gibbon ape leukemia virus; and in a region of the human immunodeficiency virus (HIV) long terminal repeat partially characterized as a negative regulatory element. The protein level and binding activity of Fos and Fos-related antigens increase rapidly after calcium ionophore treatment of a CD4+ human lymphoblast cell line, H9. These data suggest that several proteins may associate with the AP-1 binding site. Moreover, temporally regulated control of the level of each protein could represent a mechanism for modulation of these putative mediators of gene expression.
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D011232:Chemical Precipitation; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D004742:Enhancer Elements, Genetic; D006678:HIV / Q000235:genetics; D006801:Humans; D007118:Immunoassay; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos; D011519:Proto-Oncogenes; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000187:drug effects
chemical_list: D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D000001:Calcimycin; D004247:DNA