title: Metabolism of zearalenone by sow intestinal mucosa in vitro.
abstract: Homogenized intestinal mucosa samples from sows were incubated with zearalenone in the presence of NADPH or UDPGA. In addition, UDPglucuronosyltransferase activity in the microsomal fraction of mucosa was determined using 1-naphthol as substrate. In the presence of NADPH, zearalenone was reduced to both alpha- and beta-zearalenol (0.37 +/- 0.18 and 0.29 +/- 0.11 nmol/mg protein/hr in the duodenum and jejunum, respectively). The beta-isomer was the predominant metabolite. Glucuronide conjugation of zearalenone was very high compared with the level of reduction occurring (11.3 +/- 6.1 and 9.4 +/- 5.8 nmol conjugated/mg protein/hr in the duodenum and jejunum, respectively). There was no correlation between the rates of glucuronide conjugation of zearalenone and 1-naphthol, indicating that they depend upon two different isoenzymes of UDPglucuronosyltransferase.
mesh_terms: D000818:Animals; D004386:Duodenum / Q000378:metabolism; D005260:Female; D005965:Glucuronates / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D009249:NADP / Q000378:metabolism; D009284:Naphthols / Q000378:metabolism; D012118:Resorcinols / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism; D015025:Zearalenone / Q000378:metabolism*
chemical_list: D005965:Glucuronates; D009284:Naphthols; D012118:Resorcinols; D014535:Uridine Diphosphate Glucuronic Acid; C029350:1-naphthol; D009249:NADP; D015025:Zearalenone; D014453:Glucuronosyltransferase