title: Post-fusion reaction of mouse oocyte cortex to incorporated thymocyte cells.
abstract: Ovulated alcohol-activated or inactivated mouse oocytes were fused with mouse thymocytes. Activated oocytes react to the presence of foreign nuclei by forming in the peripheral cytoplasm incorporation cones. In this region the cell membrane is smooth and a cortical layer of thin filaments underlies it. It resembles the fertilization cone. In non-activated oocytes a layer of thin cortical filaments of the same thickness is formed over the foreign chromatin but the surface protuberance (cone) is absent. These results suggest that the cortical alteration in the oocyte architecture may be a general reaction of the oocyte surface to various chromatins introduced, not only to sperm chromatin. The role of oocyte activation in the evolution of these cortical changes is discussed.
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D000831:Animals, Newborn; D002459:Cell Fusion; D002843:Chromatin / Q000648:ultrastructure; D005260:Female; D006822:Hybrid Cells / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009865:Oocytes / Q000166:cytology* / Q000648:ultrastructure; D013950:Thymus Gland / Q000166:cytology*
chemical_list: D002843:Chromatin