Acylation of the 176R (19-kilodalton) early region 1B protein of human adenovirus type 5.
Antipeptide sera were prepared in rabbits against synthetic peptides corresponding to the predicted amino and carboxy termini of the early region 1B 176R (19-kilodalton [kDa]) protein of human adenovirus type 5. Both antisera specifically immunoprecipitated the 19- and 18.5-kDa forms of the 176R protein observed previously with antitumor sera. These data suggested that both species are full-length molecules of 176 residues. To identify posttranslational modifications that could explain the formation of these multiple species and possibly their known association with membranes, studies were carried out to determine whether they are glycosylated or acylated. Neither the 19- nor the 18.5-kDa species appeared to be a glycoprotein, however, they were labeled with [3H]palmitate and [3H]myristate, indicating that both species are acylated. Thus, whereas acylation does not appear to be the cause of the multiple species, it could play a role in the membrane association of these viral proteins. The acylation of 176R was found to be unusual. The fatty acid linkage was resistant to treatment with hydroxylamine or methanol-KOH, suggesting that acylation was through an amide bond. In addition, both palmitate and myristate were present in 176R, suggesting either a lack of specificity in the acylation reaction or the existence of more than one acylation site.
D000215:Acylation; D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006031:Glycosylation; D006367:HeLa Cells; D006801:Humans; D007118:Immunoassay; D009856:Oncogene Proteins, Viral / Q000032:analysis / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
D017456:Adenovirus Early Proteins; D005227:Fatty Acids; D009856:Oncogene Proteins, Viral