NMR studies of [1-2H]glucose metabolism in Zymomonas mobilis.
In complementary experiments the metabolism of [1-2H]glucose in H2O and of unlabelled glucose in 2H2O by Zymomonas mobilis was examined. The utilization of [1-2H]glucose by Z. mobilis was monitored by high-resolution 2H NMR. The deuterium-labelling pattern and stereochemistry of the ethanols produced from the metabolism of [1-2H]glucose and unlabelled glucose in 2H2O were determined by a combination of 13C and 1H NMR and selective enzyme action. The labelling patterns were explained in terms of enzyme mechanisms and stereospecificity, and metabolite enolization.
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D003903:Deuterium; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009243:NAD / Q000378:metabolism; D012984:Software; D013237:Stereoisomerism
D009243:NAD; D000431:Ethanol; D003903:Deuterium; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D005947:Glucose