title: Expression of c-mos RNA in germ cells of male and female mice.
abstract: We have investigated the cell types in mouse testis and ovary in which the c-mos protooncogene is normally transcribed. Blot hybridization analysis of electrophoretically fractionated RNAs from testes of mice with defects in germ-cell development and from prepubertal and adult mice indicated that c-mos was transcribed during male germ-cell development. Analysis of purified populations of spermatogenic cell types detected c-mos RNA in the earliest haploid postmeiotic germ cell, the round spermatid, indicating that c-mos was expressed transiently during spermatogenesis. c-mos RNA was detected by blot hybridization in the ovaries of prepubertal mice and decreased in relative concentration following gonadotropin-stimulated proliferation of granulosa cells. These results suggested that c-mos was transcribed in oocytes and were confirmed by detection of high levels of c-mos RNA in isolated grown oocytes. Thus, c-mos is expressed in both male and female germ cells, suggesting possible roles for this protooncogene in meiosis, germ-cell development, fertilization, and early embryogenesis.
mesh_terms: D000367:Age Factors; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D009866:Oogenesis; D010053:Ovary / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D017231:Proto-Oncogene Proteins c-mos; D012333:RNA, Messenger / Q000096:biosynthesis*; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism*
chemical_list: D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D017231:Proto-Oncogene Proteins c-mos