title: Myocyte morphology of free wall trabeculae in right ventricular pressure overload hypertrophy in rabbits.
abstract: Right ventricular (RV) hypertrophy and changes in mechanical properties develop in response to sustained pulmonary artery construction in rabbits. We use basilar RV free wall trabeculae from rabbits for measurements of force, shortening and sarcomere length (diffraction and/or photomicrography). With enzymes we dispersed calcium tolerant myocytes from trabeculae similar to those used for the above mechanical studies. The average weight of the normal (N) rabbits (n = 16) was 2.21 +/- 0.16(1) kg and was 2.11 +/- 0.10 kg for the rabbits with RV hypertrophy (H; n = 16). The ratio of RV free wall to total ventricular weight was 0.17 +/- 0.01 in the N and 0.31 +/- 0.02 in H hearts (P less than 0.01). Average length and width were determined from digitized measures of the projected image of 42 +/- 3 Ca2+ tolerant myocytes from each N heart and 41 +/- 3 from each H heart. Average myocyte length increased from 102.9 +/- 0.9 in N to 109.8 +/- 1.0 micron in H (6.7% above N; P less than 0.05) and average width from 15.4 +/- 0.2 to 20.0 +/- 0.2 micron (29.9% above N; P less than 0.01). Sarcomere length in these quiescent myocytes was 1.92 +/- 0.003 micron in the N and 1.90 +/- 0.004 in H (P greater than 0.05); consequently, the restoring forces in the myocytes were the same as N in H. The greater addition of parallel myofibrils than of series sarcomeres in H is important for tension generation in the presence of the increased pressure load of pulmonary artery constriction. The addition of sarcomeres in series may be important to sustain muscle shortening in H and is consistent with our measures of sarcomere shortening in N and H trabeculae.
mesh_terms: D000818:Animals; D001835:Body Weight; D006332:Cardiomegaly / Q000473:pathology* / Q000503:physiopathology; D006352:Heart Ventricles / Q000473:pathology; D008297:Male; D009119:Muscle Contraction; D009206:Myocardium / Q000473:pathology*; D009929:Organ Size; D009994:Osmolar Concentration; D011817:Rabbits; D013223:Statistics as Topic