Electron microscopic localization of cell-surface markers in tissue sections using monoclonal and gold-conjugated antibodies.
We describe a method for the ultrastructural localization of surface markers on cells in intact tissue sections. Using a primary monoclonal antibody to a surface marker followed by a second stage gold-labelled antibody, surface antigens can be specifically localized. This enables cellular interactions to be studied in solid tissues.
D000230:Adenocarcinoma / Q000276:immunology; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology; D005260:Female; D006046:Gold; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology; D008854:Microscopy, Electron; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006046:Gold