Method for exchange of the lipid environment of the membrane-bound (Mg2+ + Na+)-ATPase of Acholeplasma laidlawii B.
A method that exchanges the endogenous lipids in the environment of the membrane-bound (Mg2+ + Na+)-ATPase of Acholeplasma laidlawii B with defined exogenous lipids has been devised. Results demonstrate that 99.9% of the original membrane lipids were replaced with phosphatidylcholine and phosphatidic acid. ATPase enzyme activity was maintained throughout the substitution procedure.
D000128:Acholeplasma laidlawii / Q000378:metabolism*; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism
D008563:Membrane Lipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; C035123:magnesium sodium ATPase