Immunological effects of thalidomide. Inactivity of the drug and several of its hydrolysis products in mononucleocyte proliferation tests.
In order to study the spontaneous degradation of thalidomide (N-phthalyl-glutamic acid imide), an HPLC method for the separation of the drug and its hydrolysis products was developed. The effect of these substances upon the proliferation of lectin- and allogeneically stimulated peripheral blood mononucleocytes was studied, using the incorporation of [3H]-thymidine as a marker. In contrast to optically evaluated experiments reported earlier, no inhibition of [3H] incorporation was found, regardless of the duration, mode, or timing of stimulation.
D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D013268:Stimulation, Chemical; D013792:Thalidomide / Q000032:analysis / Q000494:pharmacology*
D013792:Thalidomide