Induction of fragile sites in fibroblasts.
A simple, reliable method for the induction of folate-sensitive fragile sites, including the fragile X, in fibroblasts is described. The method involves only the addition of 600 mg/l thymidine to cultures 24 hrs before they are harvested.
D002478:Cells, Cultured; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D005467:Floxuridine / Q000494:pharmacology; D005492:Folic Acid / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male
D005467:Floxuridine; D005492:Folic Acid