[Intracellular activity and secretion of various lysosomal glycosidases in cultured human skin fibroblasts].
The intracellular activities of four lysosomal glycosidases (alpha-L-fucosidase, beta-D-hexosaminidase, beta-D-galactosidase and beta-D-glucuronidase) in human skin fibroblasts cultured in a medium with 0.1% serum increased in a greater degree than that in a medium with 10% serum. Only two glycosidases (alpha-L-fucosidase and beta-D-hexosaminidase) were secreted by fibroblasts in the culture medium. The extracellular activity of alpha-L-fucosidase and beta-D-hexosaminidase was equivalent to 80 and 25% of their intracellular activity in serum-sufficient fibroblasts and 40 and 15%--in serum-restricted fibroblasts. These results suggest that the observer phenomena are controlled by the levels of autophagy, endocytosis and membrane recycling.
D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000378:metabolism; D006596:Hexosaminidases / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D012867:Skin / Q000201:enzymology*; D005644:alpha-L-Fucosidase / Q000032:analysis / Q000378:metabolism; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases
D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D001616:beta-Galactosidase; D005966:Glucuronidase; D005644:alpha-L-Fucosidase; D001619:beta-N-Acetylhexosaminidases
Vnutrikletochnai aktivnost' i sekretsiia nekotorykh lizosomnykh glikozidaz kul'tiviruemykh fibroblastov kozhi cheloveka.