title: Modulation of histamine release from canine fundic mucosal mast cells.
abstract: To study the control of histamine release, we developed techniques for culturing fundic mucosal mast cells. After enzyme dispersion, enrichment by elutriation, and overnight suspension culture, mast cells accounted for 30% of the cells present. Histamine release into the medium, measured by radioenzymatic assay, was stimulated by the lectin concanavalin A (Con A). Ragweed antigen released histamine in antisera-sensitized cultures. Con A-induced histamine release was enhanced by adenosine, but adenosine alone was inactive. The relative potency of adenosine analogues was consistent with interaction at an adenosine A1-receptor site. The calcium ionophore A23187 (0.1-1 microM) also induced histamine release. Phorbol esters that activate protein kinase C, such as phorbol 12-myristate 13-acetate, did not release histamine but enhanced release when added to low concentrations of A23187. In contrast, inactive phorbols, such as 4 alpha-phorbol 12,13-didecanoate, failed to enhance A23187-induced release. Parallel studies with canine hepatic mast cells yielded comparable results. We conclude that canine fundic mast cells possess receptors for immunoglobulin E and adenosine. Our data are consistent with increases in cytosolic calcium and protein kinase C activation working synergistically to stimulate fundic mast cells.
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004285:Dogs; D005110:Extracellular Space / Q000378:metabolism; D005748:Gastric Fundus / Q000166:cytology / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D005755:Gastrins / Q000494:pharmacology; D006632:Histamine / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008407:Mast Cells / Q000378:metabolism*; D010277:Parasympathomimetics / Q000494:pharmacology; D011961:Receptors, Fc / Q000378:metabolism; D017455:Receptors, IgE; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D005755:Gastrins; D010277:Parasympathomimetics; D011961:Receptors, Fc; D017455:Receptors, IgE; D003208:Concanavalin A; D000001:Calcimycin; D006632:Histamine; D000241:Adenosine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium