title: Disulphide-linked receptors for IgE on rat basophilic leukaemia cells.
abstract: Rat basophilic leukaemia (RBL) cells are known to possess three different kinds of receptors capable of interacting with IgE, which have been named R, H and 71K and which differ in apparent molecular weight (MW) as determined by SDS-PAGE. Reduction of receptors isolated from surface-iodinated RBL cells reduced the MW of 71K to a value corresponding to the MW of R without altering significantly the MW of H or R. Only a single reduction product of 71K could be detected when either surface-iodinated or 3H-leucine labelled RBL cells were used. Analysis of one- and two-dimensional tryptic peptide maps derived from surface-iodinated receptors revealed a similarity between R and 71K. The tryptic peptides of H exhibited an entirely different pattern. These results suggest that 71K consists either of two disulphide-linked R-like molecules, or of one such molecule linked to another as yet undetected polypeptide chain.
mesh_terms: D000818:Animals; D001491:Basophils / Q000276:immunology*; D004220:Disulfides; D004591:Electrophoresis, Polyacrylamide Gel; D007073:Immunoglobulin E; D007942:Leukemia, Experimental / Q000276:immunology*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000032:analysis*
chemical_list: D004220:Disulfides; D010455:Peptides; D011961:Receptors, Fc; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D007073:Immunoglobulin E