The receptor for interleukin 1 in plasma membranes of the human leukemia cell K 562: biological and biochemical characterization.
Murine interleukin 1 (IL 1) inhibited concentration dependently the proliferation of murine T cell lymphomas and the human leukemic cell line K 562. The cytostatic action of IL 1 was not associated with cytotoxicity and appeared to be irreversible. Changes in the expression of surface antigens, like a rapid decrease of transferrin receptors or, more delayed, an increase in HLA-A, B, C antigen density suggested that a differentiation step was induced by IL 1. This effect of IL 1 was a direct one and most likely mediated by a specific receptor molecule. In order to characterize the receptor for IL 1, highly purified plasma membranes from K 562 were incubated with murine IL 1, and the phosphorylation pattern of plasma membrane proteins was investigated by the addition of radiolabeled ATP. At 0 degree C, IL 1 induced the specific phosphorylation of a 41 kDa membrane protein in a time- and concentration-dependent manner. Analysis of the phosphoamino acid composition revealed that IL 1 induced specifically the phosphorylation of tyrosine residues of the 41 kDa protein. Crosslinking experiments proved that the 41 kDa protein had an IL 1 binding site, strongly suggesting that the 41 kDa protein was the receptor for IL 1 itself. Affinity labeling with an ATP-analogue showed that this protein possessed an ATP binding and cleaving site. We conclude from this that the receptor for IL 1 in the plasma membranes of K 562 is a transmembranous protein of 41 kDa, which possesses a tyrosine specific protein kinase activity with an autophosphorylating capacity.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D006680:HLA Antigens / Q000032:analysis; D007375:Interleukin-1 / Q000502:physiology*; D051379:Mice; D008970:Molecular Weight; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011971:Receptors, Immunologic / Q000032:analysis / Q000502:physiology*; D017472:Receptors, Interleukin-1; D011990:Receptors, Transferrin / Q000378:metabolism
D000954:Antigens, Surface; D006680:HLA Antigens; D007375:Interleukin-1; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011990:Receptors, Transferrin; D011505:Protein-Tyrosine Kinases