Chromosome analysis of rat tumor cell lines associated with metastatic potential and drug resistance.
Seven rat rhabdomyosarcoma cell lines, 9-4/0, F6, F8, F13, F21, R3 and R9, were cytogenetically investigated. They differed in their metastatic, tumorigenic and growth potential, and in resistance to chlorozotocin (CZT = 2,3-[2-chloroethyl]-3-nitrosoureido-beta-D-glucopyranose). No strict correlation between phenotypic features and chromosomal aberrations existed; however, rearrangements specific for each line were found. Twenty-one chromosome markers, present in a minimum of 20% of cells of a line, were observed. Chromosomes 3, 7, 10, 11 and 13, were those most often involved in marker formation. Two CZT-resistant lines, R3 and R9, showed no common aberration, but R9 which was most resistant, was shown to have a homogeneously staining region, which is usually thought to be the site of gene amplification.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D004351:Drug Resistance; D007621:Karyotyping; D008175:Lung Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000556:secondary; D009362:Neoplasm Metastasis; D051381:Rats; D011919:Rats, Inbred Strains; D012208:Rhabdomyosarcoma / Q000188:drug therapy / Q000235:genetics* / Q000556:secondary; D013311:Streptozocin / Q000031:analogs & derivatives / Q000627:therapeutic use
C007028:chlorozotocin; D013311:Streptozocin