T cell hybridomas reactive with the acetylcholine receptor and its subunits.
A panel of thirty cloned rat-mouse T cell hybridomas was prepared by fusion of acetylcholine receptor (AChR)-reactive rat T cells with the mouse thymoma BW5147. The T cell hybrids were demonstrated to be AChR reactive by their ability to secrete IL 2 in response to either AChR itself or by purified AChR subunits (alpha,beta,gamma, or delta). Various patterns of AChR subunit reactivity were observed, suggesting a predominant recognition of the alpha subunit, and also a considerable cross-reactivity from one subunit to another.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology; D000954:Antigens, Surface / Q000032:analysis; D003429:Cross Reactions; D005260:Female; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000954:Antigens, Surface; D007376:Interleukin-2; D011950:Receptors, Cholinergic