Analysis of cell surface molecules on human platelets with monoclonal antibodies. I. Identification of four platelet-specific surface molecules.
Eighteen monoclonal antibodies against human platelets were raised. Extensive serological analyses confirmed that all 18 antibodies reacted only with human cells of thrombocytic lineage. Four different molecules were identified by immunoprecipitation and SDS-PAGE analysis with these antibodies: GPIIb-IIIa as detected with the four antibodies HPL 1-4, GPIb with the twelve antibodies HPL 7-18, and two mutually distinct, newly defined molecules, gp 78 and p 151, were detected with the two remaining antibodies, HPL 5 and HPL 6, respectively.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008533:Megakaryocytes / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D010980:Platelet Membrane Glycoproteins
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006023:Glycoproteins; D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins