Attempts to obtain anti(D2 dopamine receptor) antibodies via the anti-idiotypic route.
Five stable hybridomas have been obtained that secrete monoclonal antibodies against the D2-dopamine receptor-selective drug spiperone. Each monoclonal antibody has been characterized in terms of its ability to bind a range of dopamine-receptor-selective ligands. One monoclonal antibody has been purified by Protein A affinity chromatography and used to immunize mice. Anti-idiotypic antisera and one hybridoma secreting an anti-idiotypic monoclonal antibody were obtained and shown to inhibit [3H]spiperone binding to the anti-spiperone antibody used for immunization. Neither the antisera nor the anti-idiotypic monoclonal antibody, however, inhibited binding of [3H]spiperone to D2-dopamine receptors.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D002460:Cell Line; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis*; D051379:Mice; D011954:Receptors, Dopamine / Q000276:immunology*; D017448:Receptors, Dopamine D2; D013134:Spiperone / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D013134:Spiperone