Catecholamines and Ca2+ mediate an increase in activity and reactive thiols of rat heart phosphofructokinase.
Catecholamines and Ca2+ mediate an increase in reactive-thiol status of phosphofructokinase, associated with an increase in activation of the enzyme. A correlation was observed between the number of reactive thiols and activation (r = 0.878; P less than 0.001) for 14 different treatments, with approx. 1 group per 85,000-Mr subunit becoming available over the range from the lowest to the highest state of activation.
D000215:Acylation; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology*; D007461:Iodoacetates / Q000378:metabolism; D019807:Iodoacetic Acid; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D007461:Iodoacetates; D013438:Sulfhydryl Compounds; D010732:Phosphofructokinase-1; D002118:Calcium; D019807:Iodoacetic Acid; D004837:Epinephrine