High performance liquid chromatographic separation of anabolic oestrogens and ultraviolet detection of 17 beta-oestradiol, zeranol, diethylstilboestrol or zearalenone in avian muscle tissue extracts.
A multi-residue analysis was developed to discern oestrogens (17 beta-oestradiol or zeranol) used as anabolic drugs and diethylstilboestrol (DES) from the mycotoxins zearalenone and zearalenol. A mixture of 17 beta-oestradiol, oestrone, DES, zeranol, zearalenol, zearalenone and zearalanone was analysed using a 5 micron silica column with gradient elution from hexane to hexane:methanol:2-propanol, 40:45:15 (v/v) and ultraviolet detection at 280 nm. Only six peaks were obtained since zearalenone and zearalanone had identical elution times. Minimum detectabilities were 5-10 ng for the standards. Chicken muscle tissues (2.5 g) were extracted with acetone:water (95:5, v/v). The extracts were spiked with oestradiol and zeranol at 50, 100 or 200 ppb; and DES or zearalenone at 50 ppb; cleaned-up through dual columns of basic alumina and phosphate exchanged AGMP-1 resin and analysed by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Minimum detectability was 10 ng for oestradiol, zeranol, DES or zearalenone when tissues were spiked at 50 ppb. Recoveries were greater than or equal to 86%, greater than or equal to 83%, 60% and 100% for oestradiol, zeranol, DES and zearalenone, respectively.
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004054:Diethylstilbestrol / Q000302:isolation & purification*; D004958:Estradiol / Q000302:isolation & purification*; D004967:Estrogens / Q000302:isolation & purification*; D004970:Estrone / Q000302:isolation & purification; D009132:Muscles / Q000032:analysis*; D012118:Resorcinols / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D015025:Zearalenone / Q000302:isolation & purification*; D015029:Zeranol / Q000302:isolation & purification*
D004967:Estrogens; D012118:Resorcinols; D004970:Estrone; D004958:Estradiol; D015025:Zearalenone; D004054:Diethylstilbestrol; D015029:Zeranol