Effect of alpha 2-interferon on hairy cells and cell lines: a role for type I interferon receptors and RNA synthesis.
The binding of alpha 2-interferon to highly purified plasma membrane proteins of malignant human lymphoid cells was assessed by Western Blotting. The human hairy cell leukemia cell line JOK-1 revealed three major alpha-interferon binding proteins with molecular weights of 120, 100, and 32 kD. Pretreatment of JOK-1 cells with alpha-interferon in vitro results in a disappearance of these proteins, which is in concordance with receptor down-regulation on JOK-1 cells. In a case of T chronic lymphocytic leukemic (CLL), a differential binding pattern of two proteins with 100 and 85 kD was observed, whereas a case of B-CLL did not yield any signal detection. In addition, mononuclear cells from patients with hairy cell leukemia and CLL were found to differ with respect to the in vitro incorporation of nucleic acid precursors. alpha 2-Interferon enhances [3H] uridine incorporation into hairy cells, whereas this phenomenon can be detected in CLL cells only to a much lesser extent.
D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism* / Q000494:pharmacology; D007943:Leukemia, Hairy Cell / Q000378:metabolism*; D007945:Leukemia, Lymphoid / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008970:Molecular Weight; D011485:Protein Binding; D012334:RNA, Neoplasm / Q000096:biosynthesis; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D007370:Interferon Type I; D012334:RNA, Neoplasm; D011971:Receptors, Immunologic; D017471:Receptors, Interferon