Differential association between two human MHC class I antigens and an adenoviral glycoprotein.
The glycoprotein E19, encoded in early region 3 of adenovirus-2, forms complexes with major histocompatibility complex class I antigens. As a result of the complex formation, the intracellular transport of the class I antigens is abrogated, and adenovirus-infected cells display gradually diminishing quantities of cell surface-expressed class I molecules. To assess whether the E19 protein interacts equally well with different class I antigens, the associations between the viral protein and HLA-A2 and HLA-B7 antigens have been estimated. By infecting transfected HeLa cells expressing various amounts of HLA-A2 and HLA-B7 molecules, respectively, with various infectious doses of adenovirus-2, experimental conditions could be established that allowed quantitative estimates of the interactions to be determined. It was found that HLA-A2 molecules and the E19 protein interacts with a binding constant that is more than twice as high as that for HLA-B7 antigens and the viral protein. It is suggested that the pathogenicity of the virus may be dependent on the HLA-type of the infected individual.
D017456:Adenovirus Early Proteins; D000258:Adenovirus Infections, Human / Q000276:immunology; D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D006680:HLA Antigens / Q000032:analysis*; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D011991:Receptors, Virus / Q000032:analysis; D014162:Transfection
D017456:Adenovirus Early Proteins; D006680:HLA Antigens; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D009856:Oncogene Proteins, Viral; D011991:Receptors, Virus