title: Partial characterization of synovial fluid nucleotide pyrophosphohydrolase.
abstract: Synovial fluid adenosine triphosphate pyrophosphohydrolase, an enzyme which manifests increased activity in chondrocalcinosis and osteoarthritis, was partially characterized in synovial fluids from 41 patients who had a variety of arthropathies. Activity was found to be a soluble, heat labile, and divalent cation-dependent nonspecific nucleotide pyrophosphohydrolase with pH optimum 9.0-9.5.
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000037:antagonists & inhibitors; D001172:Arthritis, Rheumatoid / Q000201:enzymology; D002128:Calcium Metabolism Disorders / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D006073:Gout / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007592:Joint Diseases / Q000097:blood / Q000201:enzymology*; D007700:Kinetics; D010003:Osteoarthritis / Q000201:enzymology; D011755:Pyrophosphatases; D013379:Substrate Specificity; D013582:Synovial Fluid / Q000201:enzymology*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
chemical_list: D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases; C019757:ATP pyrophosphohydrolase; D014532:Uridine Diphosphate Glucose