A partial genomic DNA clone for the alpha subunit of the mouse complement receptor type 3 and cellular adhesion molecule Mac-1.
A genomic clone coding for the alpha subunit of the mouse complement receptor type 3 and the cellular adhesion molecule Mac-1 has been isolated directly from a genomic library using synthetic oligonucleotide probes based on the amino-terminal amino acid sequence of the protein. The identity of the clone has been established by DNA sequencing and in vitro translation of hybrid-selected mRNA. The gene is present in a single copy in the murine genome. The region containing the amino-terminal exon has been sequenced. RNA gel blotting shows that the Mac-1 alpha-subunit mRNA is 6 kilobases in length. Mac-1 alpha-subunit mRNA is present in macrophages but not T lymphoma or L cells. During gamma interferon-stimulated maturation of the mouse premyelocytic cell line M1, Mac-1 alpha-subunit mRNA is induced. This corresponds with the tissue distribution of the Mac-1 alpha subunit, showing expression is regulated at least partially at the message level.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes; D007371:Interferon-gamma / Q000494:pharmacology; D007962:Leukocytes / Q000502:physiology*; D046911:Macromolecular Substances; D016177:Macrophage-1 Antigen; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D011951:Receptors, Complement / Q000235:genetics*; D017463:Receptors, Complement 3b; D014158:Transcription, Genetic
D000954:Antigens, Surface; D046911:Macromolecular Substances; D016177:Macrophage-1 Antigen; D012333:RNA, Messenger; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D007371:Interferon-gamma