Mode of reversible binding of neocarzinostatin chromophore to DNA: base sequence dependency of binding.
The reversible binding of neocarzinostatin chromophore to polynucleotides was studied in order to understand the molecular basis of its base sequence-specificity in DNA damage production. Studies of the spectroscopic and thermodynamic properties of chromophore-polynucleotide interactions reveal that the binding of the chromophore to poly(dA-dT) is qualitatively and quantitatively different from that to poly(dG-dC) (and poly(dI-dC]. From these and other experiments using double-stranded mixtures of homopolynucleotides, it is proposed that the observed A T specific intercalation might result from differential recognition of minor variations in the B-DNA type structure by the chromophore.
D000903:Antibiotics, Antineoplastic; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D053281:Enediynes; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D009353:Zinostatin / Q000031:analogs & derivatives
D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D011089:Polydeoxyribonucleotides; C032372:neocarzinostatin chromophore; D004247:DNA; D009353:Zinostatin