Effect of serotonin and serotonin analogues on passive membrane properties of lateral septal neurons in vitro.
In this study we have tested the effect of serotonin (5-HT) and serotonin-analogues on passive membrane properties of septal neurons in a rat brain slice. Superfusion with 5-HT hyperpolarized the membrane potential and decreased the membrane resistance of lateral septal neurons in a concentration dependent manner. The lowest effective concentration was 1 microM while maximal effects were observed with 30 microM. Hyperpolarizing responses to 5-HT persisted in the presence of haloperidol, phentolamine or ritanserin. The 5-HTIa agonist 8-hydroxy-2-[n-dipropylamino]tetralin (8OHDPAT) hyperpolarized septal neurons like 5-HT though the agonist was active at a lower concentration than 5-HT and induced longer-lasting effects. Two other 5-HT1a analogues, TVX Q 7821 and buspirone, could also mimick the effect of 5-HT. The 5-HT1b agonist 1-(m-trifluoromethylphenyl)piperazine (TFMPP) did not appreciably affect the membrane potential or resistance of septal neurons. The present results are consistent with data from binding studies and suggest that the effect of 5-HT on lateral septal neurons is at least partly mediated through 5-HT1a-like receptors.
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000818:Animals; D002065:Buspirone; D002462:Cell Membrane / Q000187:drug effects; D004553:Electric Conductivity; D005277:Fenfluramine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000187:drug effects; D012688:Septum Pellucidum / Q000187:drug effects*; D012701:Serotonin / Q000494:pharmacology*; D013764:Tetrahydronaphthalenes / Q000494:pharmacology
D011743:Pyrimidines; D011985:Receptors, Serotonin; D013764:Tetrahydronaphthalenes; D005277:Fenfluramine; D012701:Serotonin; C037762:1-(3-trifluoromethylphenyl)-2-propanol; C043077:ipsapirone; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D002065:Buspirone