N-sulfation of heparan sulfate with microsomal fraction of endometrium of rabbit uterus.
Heparan sulfate was prepared from the pronase digest of the porcine kidney and modified chemically to yield N-desulfated, N-, O-desulfated, and N- O-desulfated N-acetylated heparan sulfates. Heparan sulfate and its modifications thus obtained were used as substrates to measure the sulfotransferase activity in the microsomal fraction of the endometrium of the rabbit uterus. N-, O-Desulfated heparan sulfate was the best acceptor of sulfate from 3'-phosphoadenylyl [35S] sulfate and intact heparan sulfate was the poorest one. The products of enzymatic reaction were treated with nitrous acid and examined by gel-filtration and electrophoresis. The results indicated that sulfate was almost exclusively incorporated as N-sulfate into all the present substrates.
D000818:Animals; D004717:Endometrium / Q000201:enzymology*; D005260:Female; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006497:Heparan Sulfate / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000378:metabolism; D066298:In Vitro Techniques; D008861:Microsomes / Q000201:enzymology; D011817:Rabbits; D013379:Substrate Specificity; D013466:Sulfurtransferases / Q000378:metabolism
D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D013466:Sulfurtransferases; C047141:N,O-desulfated N-acetylated heparan sulfate; C047142:N,O-desulfated heparan sulfate; C040709:N-desulfoheparan sulfate