title: Correlations between phases of deer antler regeneration and levels of serum keratan sulfate.
abstract: A sensitive and highly specific enzyme-linked immunosorbent assay with an inhibition step was used to monitor the concentration of keratan sulfate, a cartilage-related glycosaminoglycan, in the serum of three adult male deer. During the course of one complete annual antler regeneration cycle, keratan sulfate levels were found to fluctuate predictably in relation to the growth and maturation of the antlers: levels are substantially elevated during the growth phase and drop precipitously when growth ceases and the antlers become fully mineralized. In addition, an unanticipated elevation of serum keratan sulfate was observed in early spring prior to casting of the preceding year's antlers and the initiation of regrowth. This suggests that changes in cartilage metabolism occur concomitantly, with this phase of the antlerogenic cycle. These results show predictable and physiologically regulated variation in serum keratan sulfate levels which correlate directly with specific phases of the antler regeneration cycle. Furthermore, the findings provide additional support for the assertion that measurements of keratan sulfate levels in serum can provide useful information about changes in cartilage metabolism in normal as well as diseased states.
mesh_terms: D000818:Animals; D000999:Antlers / Q000502:physiology*; D003670:Deer / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D006025:Glycosaminoglycans / Q000097:blood*; D006733:Horns / Q000502:physiology*; D007632:Keratan Sulfate / Q000097:blood*; D008297:Male; D010012:Osteogenesis; D012038:Regeneration; D012621:Seasons
chemical_list: D006025:Glycosaminoglycans; D007632:Keratan Sulfate