title: [H3]-nitrendipine binding in non-neuronal cell cultures.
abstract: 3H-Nitrendipine binding was observed in non-neuronal cells derived from fetal mouse spinal cultures grown in 10% fetal calf serum. One class of binding sites was observed with a Kd of 25 nM and a Bmax of 293 fmol/well (13 fmol/micrograms protein). These data suggest that low affinity binding sites for nitrendipine are present on non-neuronal cells.
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001665:Binding Sites; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D051379:Mice; D009474:Neurons / Q000378:metabolism; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000378:metabolism; D009568:Nitrendipine; D009836:Oligodendroglia / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009568:Nitrendipine; D009543:Nifedipine