The development of inducer and effector immune suppressor cell function in Xenopus laevis, the South African clawed toad: the effect of metamorphosis.
Thymic capacity to induce suppression of antibody production by immunized Xenopus laevis toadlet spleen fragments was tested in co-cultures for different developmental stages (Nieuwkoop, P.D. and J. Faber: Normal Table of Xenopus laevis (Daudin) (North Holland, Amsterdam) 1967). While thymuses of stages 52-54 (premetamorphosis) induced suppression, those of stages 58-62 (metamorphosis) did not. This capacity returned in metamorphic climax (stages 63-65). Tests of lectin-induced suppressor function in spleens of different developmental stages exposed the same pattern of compromised activity during metamorphosis. To test whether larval thymuses could effect suppression, rather than just induce it, antigen-activated thymuses from the different stages were co-cultured with immunized toadlet spleen fragments which had been suppressor-depleted by cyclophosphamide. Only thymuses from premetamorphic larvae suppressed. Thus, when thymic capacity to induce suppression returned in metamorphic climax, it was adult-like: it lacked effector suppressor cells.
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D006388:Hemagglutinins; D007814:Larva; D037102:Lectins / Q000494:pharmacology; D008675:Metamorphosis, Biological; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D014909:Wheat Germ Agglutinins; D014982:Xenopus laevis / Q000254:growth & development / Q000276:immunology*
D006388:Hemagglutinins; D037102:Lectins; D014909:Wheat Germ Agglutinins; D003520:Cyclophosphamide