title: Regulatory elements involved in Drosophila Adh gene expression are conserved in divergent species and separate elements mediate expression in different tissues.
abstract: Alcohol dehydrogenase (ADH) is expressed in a complex temporal and spatial pattern from tandem promoters (proximal and distal) in Drosophila melanogaster, and from two closely linked genes (Adh-1 and Adh-2) in D. mulleri. The expression patterns of Adh-1 and the proximal promoter, and Adh-2 and the distal promoter are similar, but not identical. We show that the mulleri Adh genes are appropriately expressed when introduced into the melanogaster genome, indicating that the cis- and trans-acting elements which regulate the corresponding promoters are functionally equivalent in the two species. By analyzing the expression of in vitro generated mutants of the mulleri Adh locus, we define at least three regulatory regions of the mulleri Adh genes and show that different control elements mediate the expression of Adh in different tissues.
mesh_terms: D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics; D000818:Animals; D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics; D004625:Embryo, Nonmammalian; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D007814:Larva; D009928:Organ Specificity; D014158:Transcription, Genetic
chemical_list: D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase