Lymphokines regulate expression of cell surface antigens and production of suppressor factors by murine suppressor T cell hybridomas.
Suppressor T cell hybridomas specific for L-glutamic acid 60-L-alanine30-L-tyrosine10 (GAT) elaborate monoclonal suppressor factors (TsF) and bear surface markers such as H-2, I-J, and Thy-1 antigens. Over a period of years in culture, the production of TsF by such hybridomas has been constitutive and continuous; by contrast, the expression of surface markers has declined and some have been lost. The mixture of lymphokines in the supernatant fluid from concanavalin A-activated mixtures of allogeneic splenocytes (CAS) restored surface antigen expression, and the effective agents have been identified by using purified sources of lymphokines. Murine gamma interferon (IFN gamma) induced expression of H-2 class I antigens, but not I-J or Thy-1, in the hybridoma cells. Human interleukin 2 (IL-2) induced the increased expression of I-J and Thy-1 but not H-2 class I antigens. Interestingly, production of TsF was decreased by IFN gamma treatment but increased by IL-2, indicating some measure of regulation by lymphokines of the level of a protein that is constitutively produced.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D006183:H-2 Antigens / Q000032:analysis; D006825:Hybridomas / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D013491:Suppressor Factors, Immunologic / Q000032:analysis; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*
D000954:Antigens, Surface; D006183:H-2 Antigens; D007376:Interleukin-2; D013491:Suppressor Factors, Immunologic; D007371:Interferon-gamma