Influence of long-term immobilization stress on regional blood-brain barrier permeability, cerebral blood flow and 5-HT level in conscious normotensive young rats.
Eight hours immobilization stress in young rats has increased the blood-brain barrier (BBB) permeability in 12 out of 14 brain regions studied. In the same regions cerebral blood flow (CBF) diminished by 2-37%, but the magnitude of flow reduction was not correlated with the degree of increased BBB permeability. On the other hand, a correlation was observed with increased plasma and brain 5-HT levels. The increased BBB permeability and increased 5-HT levels were prevented by pretreatment with p-CPA, indomethacin and diazepam. Cyproheptadine and vinblastine pretreatment prevented the occurrence of increased BBB permeability alone. The probable mechanism(s) underlying the breakdown of BBB permeability is discussed.
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D001921:Brain / Q000098:blood supply* / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D003533:Cyproheptadine / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D005260:Female; D010134:Fenclonine / Q000031:analogs & derivatives / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow / Q000187:drug effects; D012149:Restraint, Physical; D012701:Serotonin / Q000378:metabolism*; D012702:Serotonin Antagonists / Q000494:pharmacology; D013312:Stress, Physiological / Q000188:drug therapy / Q000503:physiopathology*; D013997:Time Factors; D014747:Vinblastine / Q000494:pharmacology
D012702:Serotonin Antagonists; C020261:4-chlorophenylalanine methyl ester; D003533:Cyproheptadine; D012701:Serotonin; D014747:Vinblastine; D003975:Diazepam; D010134:Fenclonine; D007213:Indomethacin