Difference in activation by Tris(hydroxymethyl)aminomethane of Ca,Mg-ATPase activity between young and old rat skeletal muscles.
Fresh weight of rat skeletal muscles (M. tibialis anterior) was decreased with age. Specific activity of myosin-ATPase in the homogenate was decreased significantly at later stages of age, but not Ca,Mg-ATPase activity. The activity of Ca,Mg-ATPase was activated by high concentration (more than 75 mM) of Tris(hydroxymethyl)aminomethane. The degree of the activation was observed to be an age-related change; the activation of Ca,Mg-ATPase activity in old rats was lower than that of young rats.
D000375:Aging / Q000378:metabolism*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004789:Enzyme Activation; D008297:Male; D009132:Muscles / Q000033:anatomy & histology / Q000201:enzymology*; D009929:Organ Size; D051381:Rats; D014325:Tromethamine / Q000494:pharmacology*
D014325:Tromethamine; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases