title: Serodiagnosis of lungworm infection in calves using ELISA.
abstract: Soluble antigens were prepared from adult Dictyocaulus viviparus worms and from parasitic larval stages. Circulating antibody titres against these antigens were determined during experimental and field challenge studies. Stage-specific responses were found in vaccinated and non-vaccinated calves after challenge infection. Titre-values are probably correlated with the number of larvae reaching the lungs and the number of larvae developing into later larval resp. adult stages. The ELISA-technique appeared to be useful for diagnosis of lungworm infection level in cattle herds and has been applied in a sero-epidemiological survey in the Netherlands.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D004021:Dictyocaulus / Q000276:immunology*; D004022:Dictyocaulus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007814:Larva; D009426:Netherlands; D014251:Trichostrongyloidea / Q000276:immunology*; D014611:Vaccination
chemical_list: D000906:Antibodies; D000947:Antigens, Helminth