Suicide selection of murine T helper clones specific for variable regions of the influenza hemagglutinin molecule.
A negative selection procedure has been developed to obtain murine T helper clones specific for variable regions of the influenza A hemagglutinin. T cell lines, established from mice primed by intranasal infection with X31 (H3N2) virus, were cross-stimulated with natural variant viruses of known primary sequence (either A/TEXAS/1/77 or A/ENG878/69) and proliferating cells eliminated by treatment with the cell cycle-specific drug 5-bromodeoxyuridine. After two suicide cycles, T cell lines were subtype specific and failed to recognize the natural variants. Clones were established by limiting dilution and their specificity was determined against a panel of viruses. Extensive diversity was evident in the reactivity of clones from individual donors, and two major T cell recognition sites were defined in the globular head region of the hemagglutinin molecule.
D000595:Amino Acid Sequence; D000818:Animals; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle; D002999:Clone Cells / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology*; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010641:Phenotype; D051381:Rats; D012641:Selection, Genetic; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology*
D000956:Antigens, Viral; D006183:H-2 Antigens; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D001973:Bromodeoxyuridine