Features of haemocyanin-induced suppression in vivo. Study of the fractionation of T lymphocytes involved in suppression over unsolubilized conjugated histamine columns.
Haemocyanin (KLH, 4 mg), given intraperitoneally, induces in mice a state of unresponsiveness related only to the activity of two independent pathways of suppression. The early pathway is characterized by the depression of the anti-trinitrophenyl response to TNP KLH within 24 h of KLH injection, and the ability of spleen cells from KLH-treated animals to transfer unresponsiveness in normal recipients. These features of suppression are stopped after fractionation of spleen cells from KLH-treated mice over insolubilized conjugated histamine columns. The late pathway of suppression is characterized by the nonspecificity of its effector phase, leading to a depressed anti-FLu antibody response after a challenge with TNP KLH + FLu OVA. These features, which are cyclophosphamide-resistant, are no longer active after passage through insolubilized conjugated histamine columns.
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D006433:Hemocyanins / Q000494:pharmacology*; D007108:Immune Tolerance; D007114:Immunization; D008297:Male; D051379:Mice; D011968:Receptors, Histamine / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000145:classification*; D014301:Trinitrobenzenes / Q000276:immunology
D011968:Receptors, Histamine; D014301:Trinitrobenzenes; D003520:Cyclophosphamide; D006433:Hemocyanins