A chemiluminescent immunoassay for zeranol and its metabolites.
A sensitive immunoassay is presented for the detection of the xenobiotic oestrogenic growth promotor zeranol (Ralgro) and the mycotoxin zearalanone. The detection is based on chemiluminescence using a zeranol-isoluminol conjugate. A lowest detectable amount of 2-5 pg zeranol per tube was achieved. The bovine zeranol metabolites zearalanone and taleranol can also be determined, and this has been shown in a metabolic pilot study of zeranol in veal calves. This study indicates that taleranol is a major metabolite in male veal calves, in contrast to zearalanone which is known to be the major metabolite in adult cattle.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003429:Cross Reactions; D007118:Immunoassay / Q000379:methods; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives; D008297:Male; D012118:Resorcinols / Q000032:analysis*; D015025:Zearalenone / Q000032:analysis*; D015029:Zeranol / Q000031:analogs & derivatives / Q000032:analysis*
D007202:Indicators and Reagents; D012118:Resorcinols; C032487:isoluminol; D008165:Luminol; D015025:Zearalenone; D015029:Zeranol