title: Translocation of sugars into rat liver lysosomes. Evidence against a common carrier for D-glucose and D-ribose.
abstract: A conflict exists in the literature concerning the mode of translocation of D-glucose and D-ribose across the lysosome membrane. The more rapid net uptake of ribose, when measured by the osmotic-protection technique, has been attributed either to its smaller size and lower hydrogen-bonding capacity, or to a lower affinity for a transport system shared by both sugars. The latency of acid beta-hexosaminidase in isolated rat liver lysosomes was measured after preincubation for periods up to 1 h in various solutions containing glucose and/or ribose, and in some cases sucrose. After confirmation of the superior osmotic protection afforded by glucose (than by ribose), it was shown that a solution 0.125 M in both glucose and ribose provided protection intermediate between that given by 0.25 M-glucose and that given by 0.25 M-ribose. This result is inconsistent with the common-carrier hypothesis.
mesh_terms: D000818:Animals; D001692:Biological Transport; D005947:Glucose / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D012266:Ribose / Q000378:metabolism*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D012266:Ribose; D001619:beta-N-Acetylhexosaminidases; D005947:Glucose