Suboptimal C3b/C3bi deposition and defective yeast opsonization. I. Evidence for the absence of essential co-factor activity.
Defective yeast opsonization in sera with no demonstrable abnormality of known complement components is associated with the absence of inactivity of a co-factor which enhances the deposition of C3 fragments (C3b/C3bi) derived from both classical and alternative pathways of complement activation. The factor binds strongly to zymosan at 4 degrees C and loses its biological activity completely when heated for 30 min at 56 degrees C but not when similarly heated at 50 degrees C. Sera with defective deposition of C3 fragments may be readily corrected with small quantities of sera having normal function. Sera with the defect do not correct other similar sera.
D000328:Adult; D002118:Calcium / Q000494:pharmacology; D002648:Child; D003176:Complement C3 / Q000378:metabolism; D003179:Complement C3b / Q000378:metabolism*; D003180:Complement C3b Inactivator Proteins / Q000378:metabolism; D015932:Complement C3c; D017242:Complement Factor H; D003171:Complement Pathway, Classical; D004306:Dose-Response Relationship, Immunologic; D004492:Edetic Acid / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D009895:Opsonin Proteins / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D014357:Trypsin; D015054:Zymosan
C493495:CFH protein, human; D003176:Complement C3; D003180:Complement C3b Inactivator Proteins; D009895:Opsonin Proteins; D003179:Complement C3b; D015932:Complement C3c; D017242:Complement Factor H; D015054:Zymosan; D004492:Edetic Acid; D014357:Trypsin; D002118:Calcium