Immunization of mice against blood-stage Plasmodium yoelii malaria with isoelectrically focused antigens and correlation of immunity with T-cell priming in vivo.
Mice were immunized with lethal Plasmodium yoelii blood-stage malaria antigens that had been fractionated by isoelectric focusing using a variety of Ampholines over the range pH 3 to 10. Fractions were tested for their ability to protect against live challenge and to prime for parasite-specific T-cell help. Both activities exhibited three major peaks in the pH regions 4.5, 6.5, and 8, the pH 4.5 peak being the most consistently protective. There was a significant correlation between protection and T-helper-cell priming, particularly with antigens from the first peak, suggesting that T-cell priming represents an important component of the function of some protective malaria vaccines.
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage* / Q000302:isolation & purification; D005260:Female; D007111:Immunity, Cellular; D007525:Isoelectric Focusing; D008288:Malaria / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D008297:Male; D051379:Mice; D010967:Plasmodium yoelii / Q000276:immunology*; D013347:Subcellular Fractions / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014612:Vaccines / Q000494:pharmacology
D000953:Antigens, Protozoan; D014612:Vaccines