Metallothionein-determination in biological materials: interlaboratory comparison of 5 current methods.
Five current methods of metallothionein (MT)-estimation in biological materials (Hg/TCA-, Cd/heme-, SH-assay, G 75-method and RIA) were compared. Uniform calibration was ensured by measuring the nitrogen-content of the standard-apoprotein after "Kjeldahl-digestion". Known amounts of rabbit standard Cd-MT I were recovered from identical rat S9-samples. In an overall rating, RIA and Cd/heme-assay performed best, whereas the other methods either underestimated (SH-, G 75) or overestimated (Hg/TCA) the 100%-expectation.
D000818:Animals; D002104:Cadmium; D002850:Chromatography, Gel; D006418:Heme; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008631:Mercury Radioisotopes; D008668:Metallothionein / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D013438:Sulfhydryl Compounds / Q000032:analysis; D014238:Trichloroacetic Acid
D008631:Mercury Radioisotopes; D013438:Sulfhydryl Compounds; D002104:Cadmium; D006418:Heme; D014238:Trichloroacetic Acid; D008668:Metallothionein