[Preparative variant of the purification of a trypsin-like thrombolytic enzyme from Actinomyces 771].
A simple large-scale purification scheme for a trypsin-like enzyme from Actinomyces 771 was developed using carboxylic cation exchange resin Soloze K and DEAE-cellulose. The electrophoretically homogeneous enzyme with the specific trypsin activity of 1.4 U/mg was obtained with a yield of 53%.
D000190:Actinomyces / Q000201:enzymology*; D001431:Bacteriological Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005341:Fibrinolysin / Q000302:isolation & purification*; D014357:Trypsin / Q000302:isolation & purification*
D014357:Trypsin; D005341:Fibrinolysin
Preparativnaia skhema ochistki tripsinopodobnogo tromboliticheskogo fermenta iz Actinomyces 771]