title: Different regulation of the L3T4-T cell subset by B cells in different mouse strains bearing the H-2k haplotype.
abstract: Splenic L3T4-T cells from Toxoplasma gondii-infected CBA/J (H-2k, Igh 1a) but not C3H/He (H-2k, Igh 1j) mice responded with marked antigen-specific proliferation and interleukin 2 (IL 2) production, as well as concanavalin A-induced proliferation. The proliferative response of C3H/He spleen cells could be restored in part in vitro by addition of exogenous recombinant IL 2. The observed unresponsiveness of C3H/He spleen cells was due to the release of IL 2-inhibiting factors. These factors, present in culture supernatants from antigen restimulated C3H/He spleen cells, blocked the growth of an IL 2-dependent T cell line in the presence of optimal concentration of IL 2. During the early stages of infection, C3H/He splenic macrophages lacked the capacity to present antigen. In addition a nonspecific B cell-mediated suppression of T cell proliferation was observed. In later stages of infection macrophages displayed normal antigen-presenting function in comparison with macrophages of normal uninfected mice. At this stage of infection nonspecific, minor histocompatibility restricted B cell suppression was observed. These results suggest a possible role of B cells in regulation of T cell immunity to T. gondii.
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D006183:H-2 Antigens / Q000235:genetics; D006239:Haplotypes; D007213:Indomethacin / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D013411:Sulfadiazine / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006183:H-2 Antigens; D007376:Interleukin-2; D008222:Lymphokines; D013411:Sulfadiazine; D007213:Indomethacin