Inhibition of phospholipase A2 by protein I.
The 36 kDa substrate of several tyrosine protein kinases has been shown to exist in monomeric and oligomeric (362102) forms. Partial sequence data has suggested that the oligomer, referred to as protein I, is homologous to a group of phospholipase A2 inhibitory proteins, collectively called lipocortins. In the present communication we demonstrate that protein I inhibits bovine pancreas phospholipase A2 with similar potency to that of lipocortin. Approximately 44 pmol protein I was required to produce 50% inhibition of 7.2 pmol of phospholipase A2. Inhibition of phospholipase A2 activity by calmodulin, S-100, calregulin, parvalbumin, troponin-C, or CAB-48 was not observed. These results indicate that protein I is a potent and specific inhibitor of phospholipase A2 activity, and thus shares functional homology with the lipocortin proteins. We therefore propose that this protein be named lipocortin-85.
D000595:Amino Acid Sequence; D000818:Animals; D017302:Annexins; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D010179:Pancreas / Q000201:enzymology; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D016704:Synapsins
D017302:Annexins; D002135:Calcium-Binding Proteins; D006023:Glycoproteins; D009419:Nerve Tissue Proteins; D016704:Synapsins; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2