Characterization by electrophoretic zymograms of 19 Trypanosoma cruzi clones derived from two chronic chagasic patients.
1. Electrophoretic patterns of aspartate aminotransferase, glucose-6-phosphate dehydrogenase, phosphoglucomutase, glucose-phosphate isomerase, malic enzyme and alcohol dehydrogenase have been analyzed in extracts from Trypanosoma cruzi, Tulahuén strain, 19 clones derived from isolates obtained from two chronic chagasic patients from Argentina and from Brazilian stocks Silvio X10/1 (zymodeme 1), Esmeraldo/1 (zymodeme 2), and CAN-III/1 (zymodeme 3). 2. The clones isolated from one of the patients were genetically heterogeneous. 3. Phosphoglucomutase and glucose phosphate isomerase patterns for the clones analyzed clearly differ from those of the Brazilian stocks. 4. Grouping of clones on the basis of isozyme patterns showed some correlation with that based on total DNA per organism. 5. Under the experimental conditions used, the polyacrylamide gel electrophoresis micromethod employed was advantageous over starch gel electrophoresis.
D000426:Alcohol Dehydrogenase / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis; D010733:Phosphoglucomutase / Q000032:analysis; D014349:Trypanosoma cruzi / Q000145:classification / Q000201:enzymology* / Q000235:genetics
D007527:Isoenzymes; D004247:DNA; D000426:Alcohol Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D001219:Aspartate Aminotransferases; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase