Cytoprotective effect of 16,16' dimethyl prostaglandin E2 (dm PGE2) on streptozotocin-induced biochemical alterations of Golgi-rich membrane fraction in comparison with morphology of rat liver Golgi apparatus in situ.
In 10-11 days after single, intraperitoneal injection of streptozotocin we found a lower (although not statistically significant) yield of Golgi-rich membrane fraction in comparison with control. In these rats similar to control specific activity of galactosyltransferase in nM Gal transferred per h and mg of protein, and statistically significant lower total activity of this enzyme expressed in nM Gal transferred per h and per total liver (t = 2.9666, 0.01 less than p less than 0.05) were found. Organization of Golgi apparatus in situ showed alterations that indicated suppression of secretory activity. Within the dm PGE2 protected animal group the obtained data equal control data.
D015064:16,16-Dimethylprostaglandin E2 / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D003451:Cryoprotective Agents; D005260:Female; D005615:Freezing; D005700:Galactosyltransferases / Q000378:metabolism; D006056:Golgi Apparatus / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin / Q000494:pharmacology*; D013347:Subcellular Fractions
D003451:Cryoprotective Agents; D011459:Prostaglandins E, Synthetic; D013311:Streptozocin; D005700:Galactosyltransferases; D015064:16,16-Dimethylprostaglandin E2