Embryotoxicity induced by alkylating agents. Some methodological aspects of DNA alkylation studies in murine embryos using ethylmethanesulfonate.
Synthesis and spectroscopic analysis of some alkylated DNA purine bases are described. HPLC separation methods are developed for the determination of DNA alkylation rates in mammalian embryonic tissues. Following treatment of pregnant mice with the ethylating agent ethylmethanesulfonate (EMS), an appreciable amount of alkylation (ethylation and methylation) was found in the nuclear DNA of the embryos during organogenesis. The results are discussed in context of our thesis that a certain amount of DNA alkylation in the embryos is correlated to the teratogenic potential of alkylating agents.
D000225:Adenine / Q000031:analogs & derivatives; D000478:Alkylation; D000818:Animals; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000187:drug effects*; D005020:Ethyl Methanesulfonate / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives; D008099:Liver / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D013055:Spectrophotometry, Infrared; D013329:Structure-Activity Relationship
D006147:Guanine; D004247:DNA; D005020:Ethyl Methanesulfonate; D000225:Adenine