Partial characterization of synovial fluid nucleotide pyrophosphohydrolase.
Synovial fluid adenosine triphosphate pyrophosphohydrolase, an enzyme which manifests increased activity in chondrocalcinosis and osteoarthritis, was partially characterized in synovial fluids from 41 patients who had a variety of arthropathies. Activity was found to be a soluble, heat labile, and divalent cation-dependent nonspecific nucleotide pyrophosphohydrolase with pH optimum 9.0-9.5.
D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000037:antagonists & inhibitors; D001172:Arthritis, Rheumatoid / Q000201:enzymology; D002128:Calcium Metabolism Disorders / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D006073:Gout / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007592:Joint Diseases / Q000097:blood / Q000201:enzymology*; D007700:Kinetics; D010003:Osteoarthritis / Q000201:enzymology; D011755:Pyrophosphatases; D013379:Substrate Specificity; D013582:Synovial Fluid / Q000201:enzymology*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases; C019757:ATP pyrophosphohydrolase; D014532:Uridine Diphosphate Glucose