The subdivision of the T4 (CD4) subset on the basis of the differential expression of L-C/T200 antigens.
The T4 (CD4) subset of T lymphocytes has been subdivided into two major subsets, a suppressor/inducer subset (T4+,2H4+) and a helper subset (T4+,2H4-) on the basis of the differential expression of the L-C/T200 (CD45) antigens. The 2H4 antigen itself comprises at least three distinct polypeptides at 125,200, and 220 X 10(3) Mr, of which the 200 and 220 X 10(3) Mr polypeptides constitute the highest Mr isoforms of a pool of five distinct L-C/T200 antigens. The T4+,2H4+ subset expresses at least four of these isoforms at 180, 190, 200, and 220 X 10(3) on the cell surface, while the T4+,2H4- subset expresses only the 180 and 190 X 10(3) Mr forms. Pulse-chase analysis and endoglycosidase treatment revealed that the 125 X 10(3) Mr chain of the 2H4 antigen is nonglycosylated, while the 200 and 220 X 10(3) polypeptides are structurally related and derived by N- and O-linked glycosylation from two nascent subunits at 150 and 160 X 10(3) Mr. The function of the T4+,2H4+ subset could be blocked only by an antibody reactive with the L-C/T200 isoforms enriched with O-linked oligosaccharides at 200 and 220 X 10(3) Mr.
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D006023:Glycoproteins / Q000276:immunology; D006026:Glycoside Hydrolases; D006649:Histocompatibility Antigens / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D017493:Leukocyte Common Antigens; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification; D050378:T-Lymphocytes, Regulatory / Q000145:classification
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D006023:Glycoproteins; D006649:Histocompatibility Antigens; D017493:Leukocyte Common Antigens; D006026:Glycoside Hydrolases