The effects of trypsin, plasmin, chymotrypsin and thrombin on human heart adenylate cyclase activity.
The authors report the effects of four proteases (trypsin, plasmin, chymotrypsin and thrombin) on human heart adenylate cyclase (HHAC) activity. Trypsin and plasmin inhibited HHAC at concentrations higher than 0.3 and 1 microgram/ml, respectively. Chymotrypsin had a biphasic effect, with a stimulation (from 0.5 to 10 micrograms/ml) followed by an inhibition at higher concentrations. Maximal stimulation was obtained at 3 micrograms/ml and averaged 67.2 +/- 5.4%. Thrombin had no significant effect at concentrations up to 1 mg/ml. These data indicate that proteases might regulate HHAC and therefore influence cardiac function.
D000262:Adenylyl Cyclases / Q000032:analysis*; D002918:Chymotrypsin / Q000494:pharmacology; D005341:Fibrinolysin / Q000494:pharmacology; D006801:Humans; D009206:Myocardium / Q000201:enzymology*; D013917:Thrombin / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D002918:Chymotrypsin; D014357:Trypsin; D013917:Thrombin; D005341:Fibrinolysin; D000262:Adenylyl Cyclases