Relationship of the distribution of Leu-2+ cells with suppressor cell activities in the spleen and lymph-nodes from gastric cancer.
Tissue distributions of Leu-2+ cells in the spleen and draining lymph-nodes in cases of clinical gastric cancer were investigated, with special reference to suppressor cell function. Significantly higher Concanavalin-A (Con-A) induced suppressor cell activities were evident in spleen cells (SCs), as compared with peripheral blood lymphocytes (PBLs). As for the tissue distribution, the proportion of Leu-2+ (cytotoxic/suppressor) cells within Leu-1+ cells was higher in the spleen than in the lymph-nodes without metastasis. On the other hand, in lymph-nodes with metastasis, the enhanced spontaneous suppressor cell activity was noted. In addition, the proportion of Leu-2+ cells within Leu-1+ cells was the greatest in the lymph-nodes with metastasis, among the lymphoid organs tested. In lymph-nodes without metastasis, lower suppressor cell activities were noted, and numerous Leu-3+ (helper/inducer) cells were present, while Leu-2+ cells were less frequent. NK cell activity against K-562 cells was enhanced by elimination of Leu-2+/OKT-8+ cells with complement-mediated lysis. These results suggest that Leu-2+ cells located in the spleen and lymph-nodes with metastasis may predominantly act as suppressor cells and interact with effector cells.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D003208:Concanavalin A; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology*; D008207:Lymphatic Metastasis; D008875:Middle Aged; D013154:Spleen / Q000276:immunology*; D013274:Stomach Neoplasms / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000145:classification* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003208:Concanavalin A