Binding of fructose-1,6-bisphosphate to yeast phosphofructokinase.
Binding of fructose-1,6-bisphosphate to yeast phosphofructokinase (EC 2.7.1.11) was measured in a concentration range of 5 to 200 microM of fructose-1,6-bisphosphate with the ultrafiltration technique. At saturation two molecules of fructose-1,6-bisphosphate are bound per subunit of the octameric enzyme. Two distinct types of binding sites have been observed. The high affinity sites (KH = 32.7 +/- 5 microM) exhibit a hyperbolic response in respect to the binding of fructose-1,6-bisphosphate, the low affinity sites (KL = 57.2 +/- 6 microM) show significant positive cooperativity.
D005635:Fructosediphosphates / Q000378:metabolism*; D006598:Hexosediphosphates / Q000378:metabolism*; D007700:Kinetics; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D010732:Phosphofructokinase-1; C029063:fructose-1,6-diphosphate