A cell hybrid and recombinant DNA library that facilitate identification of polymorphic loci in the vicinity of the Huntington disease gene.
Somatic cell hybrids were selected that retain a derivative chromosome 5 from an individual in which the p15.1-pter segment of chromosome 5 is replaced with the p15.1-pter segment of chromosome 4. Hybrids that retain this derivative chromosome exclusively were found to be positive for G8, a DNA marker closely linked to the Huntington disease gene on chromosome 4p. From one such hybrid, a segregant was isolated that had deleted the entire q arm of the derivative chromosome but retained the p arm intact as its only detectable human DNA. A complete recombinant DNA library was prepared from this cell line, and the inserts in approximately 1/3 of the recombinant phage with human DNA were shown to be derived from 4pter-4p15.1, which represents only approximately 1% of the total human genome. The cell hybrid and DNA library represent a rapid and efficient means to identify and isolate many polymorphic DNA markers close to and flanking the Huntington disease gene.
D000818:Animals; D002871:Chromosome Banding; D002894:Chromosomes, Human, Pair 4; D006224:Cricetinae; D003412:Cricetulus; D004274:DNA, Recombinant; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D006822:Hybrid Cells; D007621:Karyotyping; D011110:Polymorphism, Genetic
D004274:DNA, Recombinant; D005819:Genetic Markers