Chromosomal assignments of genes coding for human leukocyte common antigen, T-200, and lymphocyte function-associated antigen 1, LFA-1 beta subunit.
Five hybrids reactive with monoclonal antibodies against human leukocyte common antigen (T-200) and/or lymphocyte function-associated antigen 1 (LFA-1) beta subunit were obtained from the fusion of human blood lymphocytes or T-cell chronic lymphocytic leukemia cells with BW5147 mouse T-cell leukemia cells. Chromosome analyses of 20 clones showed concordance between the presence of human chromosomes 1 and 21 and the expression of T-200 and LFA-1 beta subunit, respectively. Confirmation of human chromosomes in the hybrids was made by the electrophoretic analyses of phosphoglucomutase for chromosome 1 and superoxide dismutase for chromosome 21. The results suggested that the presence of human chromosomes 1 and 21 was essential for the expression of T-200 and LFA-1 beta subunit, respectively.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002891:Chromosomes, Human, Pair 21; D005796:Genes; D005819:Genetic Markers; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis; D017493:Leukocyte Common Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D008214:Lymphocytes / Q000032:analysis; D051379:Mice
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005819:Genetic Markers; D006649:Histocompatibility Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D017493:Leukocyte Common Antigens