Adult schistosome cDNA libraries as a source of antigens for the study of experimental and human schistosomiasis.
Protective immunity has been demonstrated in experimental schistosomiasis and is also believed to occur in man. It can be mediated by antibodies from infected animals or animals immunized with attenuated organisms. Recombinant Escherichia coli synthesizing antigenic polypeptides from the three principal species of schistosome that infect man, Schistosoma mansoni, S. japonicum and S. haematobium, have been constructed. Libraries of adult worm cDNA were prepared from each species in the expression vector lambda gt 11 and directly screened with antibodies from animals experimentally immunized with S. mansoni and S. japonicum and from humans infected with S. haematobium. The S. mansoni clones have been analysed in greatest detail. At least four different types of clones were identified. All the detected recombinant polypeptide antigens were recognised by antibodies from chronically infected mice and most were also recognised by antibodies from mice immunized with attenuated cercariae and anti-surface membrane antibodies. Clones synthesizing species-specific antigens for both S. mansoni and S. japonicum were identified by simultaneous screening of both libraries. At least three types of S. haematobium clones were identified by screening with human infection serum, most of which were species-specific. All the antigens were in the form of fusion peptides with E. coli beta-galactosidase and their expression was induced by isopropylthiogalactopyranoside. Since known protective monoclonal antibodies recognise highly glycosylated membrane proteins which cannot be identified in the form of nascent polypeptides, the direct identification of polypeptide antigens defined by their reactivity, as reported here, is an essential step in producing reagents by recombinant DNA technology, suitable for vaccination and diagnosis.
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012547:Schistosoma / Q000235:genetics / Q000276:immunology*; D012548:Schistosoma haematobium / Q000235:genetics / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology; D012550:Schistosoma mansoni / Q000235:genetics / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology*; D012553:Schistosomiasis haematobia / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D004274:DNA, Recombinant; D007106:Immune Sera; D012333:RNA, Messenger; D004247:DNA