title: The suppressive effect of tape-stripping treatment of guinea-pig skin on the induction of contact sensitivity by intradermal injection of haptenated epidermal cells.
abstract: Contact sensitivity (CS) to 2,4-dinitrochlorobenzene (DNCB) was produced in inbred JY1-strain guinea pigs by the intradermal injection of epidermal cells (ECs) prepared from DNCB-painted skin (DNP-ECs). When the site of DNP-EC-induced CS was pretreated by tape stripping, the rate and intensity of the challenge reactions to DNCB were diminished. The ability of DNP-ECs to induce CS returned to normal when normal peritoneal macrophages together with DNP-ECs were administered into the stripped skin. Normal ECs had a similar effect. Using either anti-Ia antiserum and complement or allogeneic ECs (strains 2 and 13), Ia-positive cells among the ECs (presumably Langerhans cells) were found to be essential for the recovery of CS. Tape-stripping treatment also resulted in the development of immunological tolerance, as assessed by subsequent painting with a sensitizing dose of DNCB. These findings suggest that the immunological function of the mononuclear-phagocyte system in the dermis may be impaired when the epidermal surface is markedly disturbed by tape-stripping treatment.
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology*; D004137:Dinitrochlorobenzene / Q000276:immunology; D004817:Epidermis / Q000276:immunology*; D006168:Guinea Pigs; D006241:Haptens / Q000276:immunology; D007108:Immune Tolerance; D007116:Immunization, Passive; D007801:Langerhans Cells / Q000276:immunology; D008297:Male; D010586:Phagocytes / Q000276:immunology
chemical_list: D004137:Dinitrochlorobenzene; D006241:Haptens; D000251:Adenosine Triphosphatases