title: Siderophore production by Salmonella typhi.
abstract: This study was initiated to determine the mechanism of iron-uptake in Salmonella typhi. When stressed for iron, microorganisms produce siderophores to obtain the necessary nutrient. Generally two types of siderophores exist: the phenolate-type predominantly produced by bacteria and the hydroxamate-type commonly secreted by fungi. Results of this investigation showed that S. typhi produced siderophores of the phenolate-type since culture supernatant of the organism grown under iron-deprivation supported the growth of the phenolate-dependent auxotroph. The culture supernatant when extracted for phenolate siderophores, also supported the growth of the phenolate auxotroph but not the hydroxamate auxotroph. Production of phenolate-type siderophores were further confirmed using biochemical assays. These results showed that S. typhi utilized the high-affinity iron transport system to obtain the necessary iron.
mesh_terms: D001173:Arthrobacter / Q000187:drug effects / Q000254:growth & development; D001681:Biological Assay; D002712:Chlorides; D005290:Ferric Compounds / Q000378:metabolism; D006877:Hydroxamic Acids / Q000032:analysis; D062385:Hydroxybenzoates / Q000378:metabolism / Q000494:pharmacology; D007476:Ionophores / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000378:metabolism* / Q000494:pharmacology; D012485:Salmonella typhi / Q000378:metabolism*; D017262:Siderophores
chemical_list: D002712:Chlorides; D005290:Ferric Compounds; D006877:Hydroxamic Acids; D062385:Hydroxybenzoates; D007476:Ionophores; D007502:Iron Chelating Agents; D017262:Siderophores; D007501:Iron; C017616:phenolic acid; C024555:ferric chloride