Does 2-mercaptoethane sulphonate (mesna) prevent cyclophosphamide and azathioprine induced immunosuppression? In vitro studies.
The effect of 2-mercaptoethane sulphonate (mesna) on the inhibition of the human MLR by 4-hydroxycyclophosphamide or azathioprine was studied. 4-Hydroxycyclophosphamide (34 microM) completely inhibited the MLR and this inhibition was unaffected by 122 microM of 2-mercaptoethane sulphonate or its disulphide. At high concentrations (mM) 2-mercaptoethane sulphonate inhibited the MLR reaching 85% at 24 mM. 2-Mercaptoethane sulphonate (15-122 microM) had no effect on azathioprine (36 microM) inhibition. The results of these in vitro studies suggest that 2-mercaptoethane sulphonate does not interfere with cyclophosphamide or azathioprine induced suppression of cellular immunity.
D001379:Azathioprine / Q000037:antagonists & inhibitors*; D002855:Chromatography, Thin Layer; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors; D006801:Humans; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D008623:Mercaptoethanol / Q000031:analogs & derivatives*; D015080:Mesna / Q000031:analogs & derivatives / Q000494:pharmacology*
C012358:4-hydroxycyclophosphamide; D008623:Mercaptoethanol; C027032:2,2'-dithiodiethanesulfonic acid; D003520:Cyclophosphamide; D001379:Azathioprine; D015080:Mesna