Structural analysis of the carbohydrate chains of beta-N-acetylhexosaminidases from bovine brain.
The oligosaccharide structures of bovine brain beta-N-acetylhexosaminidases A and B (EC 3.2.1.30) were studied at the glycopeptide level by employing 500 MHz 1H-n.m.r. spectroscopy and methylation analysis involving g.l.c.-m.s. More than 90% of the chains were found to be of the oligomannoside type, containing, on average, five to six mannose residues. Biantennary N-acetyl-lactosamine-type chains terminated in N-acetylneuraminic acid were found to comprise the remaining 5-10% of the total carbohydrate. The isoenzyme forms A and B do not differ from each other in the structure of their carbohydrate moiety, but do deviate in carbohydrate content and, in consequence, in the number of carbohydrate chains per molecule.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002241:Carbohydrates / Q000032:analysis*; D002417:Cattle; D006020:Glycopeptides / Q000032:analysis; D006596:Hexosaminidases; D007527:Isoenzymes; D009682:Magnetic Resonance Spectroscopy; D001619:beta-N-Acetylhexosaminidases
D002241:Carbohydrates; D006020:Glycopeptides; D007527:Isoenzymes; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases