Effects of electrical stimulation and tetrodotoxin paralysis on expression of muscle-specific isozymes of four enzymes in aneurally cultured embryonic rat muscle.
We studied the effect of electrical stimulation and a sodium channel blocker (tetrodotoxin) on the expression of muscle-specific isozymes of creatine kinase, glycogen phosphorylase, phosphoglycerate mutase, and lactate dehydrogenase in aneurally cultured embryonic rat muscle. Muscle contractile activity slightly accelerated the accumulation of muscle-specific isozyme of creatine kinase in early cultures (4 days of experiment), but no increase in the expression of muscle-specific isozymes of any enzyme was present in older cultures (11 days of experiment). We conclude that muscle contractile activity is not a main regulator of isozyme maturation in this system.
D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000096:biosynthesis; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000096:biosynthesis; D004558:Electric Stimulation; D005333:Fetus; D007527:Isoenzymes / Q000096:biosynthesis*; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis*; D009132:Muscles / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D006005:Phosphorylases / Q000096:biosynthesis; D051381:Rats; D013779:Tetrodotoxin / Q000494:pharmacology*; D014166:Transferases / Q000096:biosynthesis*
D007527:Isoenzymes; D013779:Tetrodotoxin; D007770:L-Lactate Dehydrogenase; D014166:Transferases; D006005:Phosphorylases; D003402:Creatine Kinase; D001731:Bisphosphoglycerate Mutase