Isoenzyme analyses of Malaysian Schistosoma, S. mekongi and S. japonicum by isoelectric focusing in polyacrylamide gel.
Isoenzyme patterns of adult Malaysian Schistosoma, S. mekongi and S. japonicum strains were analysed by isoelectric focusing (IEF) in polyacrylamide gel. Enzyme patterns obtained from Malaysian Schistosoma homogenates differed from those of S. mekongi and S. japonicum strains. Malaysian Schistosoma was found to differ from S. japonicum by 8 enzymes, namely phosphoglucomutase, phosphoglucoisomerase, malate dehydrogenase, acid phosphatase, hydroxy-butyrate dehydrogenase, hexokinase and alkaline phosphatase, and from S. mekongi by phosphoglucomutase, malate dehydrogenase, aldolase and alkaline phosphatase. These results and the distinct biology of the parasite suggest that Malaysian Schistosoma is a new species in the S. japonicum complex.
D000135:Acid Phosphatase / Q000032:analysis; D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006593:Hexokinase / Q000032:analysis; D006884:Hydroxybutyrate Dehydrogenase / Q000032:analysis; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis; D008297:Male; D051379:Mice; D010733:Phosphoglucomutase / Q000032:analysis; D012547:Schistosoma / Q000145:classification / Q000201:enzymology*; D012549:Schistosoma japonicum / Q000201:enzymology*
D007527:Isoenzymes; D006884:Hydroxybutyrate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase; D006593:Hexokinase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005634:Fructose-Bisphosphate Aldolase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase