title: [Expression of the chloramphenicol acetyltransferase gene is under control of various promoters of E. coli and phage lambda].
abstract: Plasmids have been constructed with the structural region of the cat gene being under the control of the lactose (lacUV5), tryptophane (trpOP), operons of Escherichia coli, the hybrid trp-lac (tac) promoter and early bacteriophage lambda promoters (PL, PR and PLIT). The expression of chloramphenicolacetyltransferase gene in Escherichia coli cells harbouring such recombinant plasmids and pBR325 as well has been examined by determining the chloramphenicol resistance and studying the enzyme activity of Cm-acetylase. A high level of enzyme synthesis is connected with transcription from PL, PR and tac promoters.
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase
vernacular_title: Ekspressiia gena khloramfenikolatsetiltransferazy pod kontrolem nekotorykh promotorov E. coli i bakteriofaga lambda.