Studies of the biosynthesis of the cation-dependent mannose 6-phosphate receptor in murine cell lines.
We have studied the biosynthesis of the cation-dependent mannose 6-phosphate receptor in murine BW5147 lymphoma cells and MOPC 315 plasmacytoma cells. The cells were labeled with [35S]methionine or [2-3H]mannose and the receptor immunoprecipitated with an anti-receptor antiserum. The receptor was first detected as a glycoprotein with an apparent molecular mass of 40 kDa. This intermediate was rapidly processed to a mature form which was stable during 22 h of chase. In these cells, the mature receptor has an apparent molecular mass of 43 kDa. The 3-kDa increase occurs as a result of processing of Asn-linked high-mannose oligosaccharides to complex-type units.
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line; D006596:Hexosaminidases / Q000494:pharmacology; D007700:Kinetics; D008358:Mannose / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis; D017527:Receptor, IGF Type 2; D014316:Tritium
D002352:Carrier Proteins; D009844:Oligosaccharides; D017527:Receptor, IGF Type 2; D014316:Tritium; D008715:Methionine; D006596:Hexosaminidases; D008358:Mannose