Damages induced in lambda phage DNA by enzyme-generated triplet acetone.
Exposure of lambda phage to triplet acetone, generated via the oxidation of isobutanal by peroxidase, leads to genome lesions. The majority of these lesions are detected as DNA single-strand breaks only under alkaline conditions, and so true breaks do not occur. Also, no sites sensitive to UV-endonuclease from Micrococcus luteus were found in DNA from treated phage. The participation of triplet acetone in the generation of such DNA damage is discussed.
D000433:1-Propanol / Q000494:pharmacology; D000096:Acetone / Q000031:analogs & derivatives*; D000447:Aldehydes / Q000378:metabolism; D010582:Bacteriophage lambda / Q000187:drug effects* / Q000235:genetics; D002374:Catalase / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D003500:Cyclization; D004279:DNA, Viral / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004706:Endodeoxyribonucleases; D006735:Horseradish Peroxidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009097:Multienzyme Complexes / Q000378:metabolism; D009699:N-Glycosyl Hydrolases; D010084:Oxidation-Reduction; D020005:Propanols; D013482:Superoxide Dismutase / Q000494:pharmacology
D000447:Aldehydes; D004279:DNA, Viral; D009097:Multienzyme Complexes; D020005:Propanols; C037881:triplet acetone; D000096:Acetone; C032219:UV endonuclease; D000433:1-Propanol; C017439:isobutyraldehyde; D006735:Horseradish Peroxidase; D002374:Catalase; D013482:Superoxide Dismutase; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; D009699:N-Glycosyl Hydrolases