[The use of a model water-lipid system for the study of the electric function of Ca2+, Mg2+-ATPase of the sarcoplasmic reticulum].
The method of dynamic capacity in the model organic phase-water system was used to investigate a possibility of studying the electrical function of Ca2+,Mg2+-ATPase from sarcoplasmic reticulum of the rabbit hind limb skeletal muscles. Decane and decane solution of azolectin were used as an organic phase. It is stated that in the model systems the sarcoplasmic reticulum Ca2+,Mg2+-ATPase did not cause ATP-dependent changes in the boundary Volta potential (delta phi) irrespective of the presence of polyvalent cation chelates in the organic phase. The fragmented sarcoplasmic reticulum is able of realizing Mg-ATP, Ca2+-dependent generation of delta phi only with phospholipids present in the organic phase. It is supposed that generation of delta phi of the fragmented sarcoplasmic reticulum is due to the active transport of calcium ions by the reticulum Ca2+,Mg2+-ATPase.
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000502:physiology*; D008055:Lipids; D008564:Membrane Potentials; D008954:Models, Biological; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D014867:Water
D008055:Lipids; D014867:Water; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases
Ispol'zovanie model'noĭ vodno-lipidnoĭ sistemy dlia izucheniia élektricheskoĭ funktsii Ca2+, Mg2+-ATP-azy sarkoplazmaticheskogo retikuluma.