title: Monoclonal antibodies to the extracellular glucosyltransferases from Streptococcus sobrinus 6715.
abstract: Murine monoclonal antibodies (MAbs) were raised against the glucosyltransferases (GTFs) of Streptococcus sobrinus 6715. The antibody panels included MAbs raised against the primer-independent, soluble product enzyme (GTF-Si) which did not cross-react with other GTFs, as well as MAbs raised against the primer-dependent, soluble product enzyme (GTF-Sd) which recognized both GTF-Si and GTF-Sd, thus indicating that these catalytically distinct enzymes share epitopes. MAbs raised against GTF-I recognized several forms of GTF-I and did not cross-react with the GTF-S enzymes. None of the MAbs recognized the major glucan-binding protein of S. sobrinus. Two MAbs inhibited glucan synthesis, one blocking primer synthesis by GTF-Si by 89% and the second inhibiting that by GTF-I by 92%.
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000276:immunology; D005110:Extracellular Space / Q000201:enzymology; D005964:Glucosyltransferases / Q000276:immunology*; D007163:Immunosorbent Techniques; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000276:immunology; D008970:Molecular Weight; D013291:Streptococcus / Q000201:enzymology / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007527:Isoenzymes; D005964:Glucosyltransferases