Preliminary characterization of a Chinese hamster ovary cell glycosylation mutant isolated by screening for low intracellular lysosomal enzyme activity.
A novel screening procedure was developed for isolating Chinese hamster ovary cell mutants altered in the early steps of the biosynthesis of asparagine-linked glycoproteins. This procedure identifies cells with low intracellular levels of two lysosomal hydrolases, beta-glucuronidase and alpha-iduronidase. One mutant cell line isolated in this way, CHB 11-1-3, has low intracellular levels of seven lysosomal enzymes as compared to wild-type cells. Although CHB 11-1-3 synthesizes mannosylphosphoryldolichol and [Man]5[NAcGlcNH2]2-P-P-lipid, it fails to utilize these lipid intermediates to make normal amounts of [Glc]3[Man]9[NAcGlcNH2]2-P-P-lipid. As a consequence of this glycosylation defect, this mutant transfers oligosaccharides of a different structure than wild type to the lysosomal enzyme beta-hexosaminidase. In addition, it underglycosylates its proteins.
D000818:Animals; D002352:Carrier Proteins / Q000502:physiology; D002460:Cell Line; D006224:Cricetinae; D005966:Glucuronidase / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D006031:Glycosylation; D007068:Iduronidase / Q000235:genetics; D008247:Lysosomes / Q000201:enzymology*; D008360:Mannosephosphates / Q000502:physiology; D009154:Mutation; D011499:Protein Processing, Post-Translational; D017527:Receptor, IGF Type 2
D002352:Carrier Proteins; D006023:Glycoproteins; D008360:Mannosephosphates; D017527:Receptor, IGF Type 2; D005966:Glucuronidase; D007068:Iduronidase