Establishment of five human malignant non-T lymphoid cell lines and mixed lymphocyte-tumor reaction.
Five human malignant non-T lymphoid cell lines have been established. THP-4 and THP-7 were derived from two children with common type acute lymphoblastic leukemia (ALL), THP-6 and THP-8 from two children with null cell type ALL and THP-9 from a child with malignant lymphoma. THP-4 was positive for cytoplasmic mu, B4, common ALL antigen (CALLA) and Ia-like antigen (Ia), THP-7 positive for B4, CALLA and Ia, THP-8 positive for B4 and Ia, and THP-9 positive for surface mu-lambda, B1, B4, CALLA and Ia. No antigenic determinants for monoclonal antibodies used were detected on THP-6. THP-4, THP-7, THP-8 and THP-9 were able to stimulate allogeneic lymphocytes vigorously. THP-6 without Ia stimulated not only allogeneic lymphocytes, but also autologous ones. THP-7 did not stimulate autologous lymphocytes.
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology / Q000502:physiology; D002455:Cell Division; D002460:Cell Line; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology* / Q000503:physiopathology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008215:Lymphocytes, Null / Q000473:pathology / Q000502:physiology; D008223:Lymphoma / Q000473:pathology* / Q000503:physiopathology; D015260:Neprilysin; D011971:Receptors, Immunologic / Q000032:analysis; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D011971:Receptors, Immunologic; D015260:Neprilysin