Isolation of beef brain phosphonolipids by thin layer chromatography: their identification and silicic acid column chromatographic separation.
The isolation and identification of the phosphonolipids from beef brain--right lobe, left lobe and cerebellum--is herein reported. The phosphonolipids were isolated by preparative thin layer chromatography (TLC) using the solvent system methanol/water (2:1, v/v) and identified preliminarily by TLC. The isolated phosphonolipids were subjected to silicic acid column chromatographic separation and the thus separated phosphonolipids were identified by TLC, nitrogen-phosphorus determinations and IR spectroscopy.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002855:Chromatography, Thin Layer; D010743:Phospholipids / Q000032:analysis*; D013055:Spectrophotometry, Infrared
D010743:Phospholipids; C028299:phosphonolipids