Analysis of azide-insensitive Ca2+-dependent ATPase activity in atrial specimens from patients with coronary or valvular heart disease.
The activity of the azide-insensitive Ca2+-dependent ATPase (highly enriched in myofibrillar ATPase activity) was studied in specimens of both right and left atria which were taken from patients with ischemic and/or valvular heart disease during coronary by pass and/or valvular substitution. A significantly lower enzymatic activity was found in atrial specimens from patients with left ventricular heart failure in comparison to the atrial fragments obtained from the patients with normal heart function. Such an inhibition reflected a significant increase in the Km of the enzyme for ATP and was associated with a concomitant reduction in Vmax, both more evident in the left atrial fragments. Moreover, tissue homogenates of atrial specimens from failing hearts exhibited a lower protein SH group content when compared to the atrial homogenates from the heart with normal left ventricular heart function.
D001386:Azides / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D001026:Coronary Artery Bypass; D003327:Coronary Disease / Q000201:enzymology* / Q000601:surgery; D006325:Heart Atria / Q000201:enzymology; D006349:Heart Valve Diseases / Q000201:enzymology* / Q000601:surgery; D006801:Humans; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology*; D009210:Myofibrils / Q000201:enzymology
D001386:Azides; D000252:Calcium-Transporting ATPases