[Importance of secretory IgA in human body fluids. I. Development of an enzyme immunoassay for secretory IgA].
This paper describes an enzyme-immunoassay for the quantitative estimation of secretory IgA(S-IgA). As solid phase a monoclonal antibody against human secretory component was used, a polyclonal peroxidase labeled anti-IgA serum as conjugate. Thus, the method was specific for S-IgA. The test was done in microtiter plates, the upper limit was 0.5 ng S-IgA/ml. The standards of our test system ran in normal ranges, the coefficients of variation were 6.9% in the within-day-test and 12.2% in the day-to-day-test. The described test system is effective and sensitive. It is suitable for clinical use.
D000911:Antibodies, Monoclonal; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007071:Immunoglobulin A, Secretory / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory
Zur Bedeutung von sekretorischem IgA in menschlichen Körperflüssigkeiten. I. Aufbau eines Enzymimmunassays für sekretorisches IgA.