[Modification of the functional properties of sarcoplasmic reticulum Ca-ATPase in hypercholesterolemia].
Using alamethicin, permitting the measurement of genuine catalytic enzyme activity, hypercholesterolemia was shown to cause a 10-30% reduction of specific Ca-ATPase activity registered at 37 degrees C and the shift of Arrhenius plot in 20-30 degrees C temperature range. Reconstruction of delipidated Ca-ATPase of sarcoplasmic reticulum membranes by egg lecithin in animals with hypercholesterolemia does not lead to the recovery of Arrhenius plot. The data obtained demonstrate that modification of temperature-dependent Ca-ATPase activity in hypercholesterolemia is associated with the changes in the polypeptide with a catalytic function and is not induced by the changes in phospholipid enzyme surroundings.
D000408:Alamethicin / Q000494:pharmacology; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002384:Catalysis; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D006937:Hypercholesterolemia / Q000201:enzymology*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology*; D013696:Temperature
D002791:Cholesterol, Dietary; D008563:Membrane Lipids; D000408:Alamethicin; D000252:Calcium-Transporting ATPases
Modifikatsiia funktsional'nykh svoĭstv Ca-ATFazy sarkoplazmaticheskogo retikuluma pri giperkholesterinemii.