Membrane capacity measurements suggest a calcium-dependent insertion of synexin into phosphatidylserine bilayers.
The mechanism by which synexin mediates calcium-dependent aggregation of medullary cell chromaffin granules and fusion of granule ghosts involves specific interactions with the lipid component of the membrane. To study the details of these interactions we measured synexin-induced changes in capacitance of phosphatidylserine bilayers formed at the tip of a patch pipet using the double-dip method. Provided calcium was present in the solution filling the pipet (10-50 mM) stable phosphatidylserine bilayers were easily formed. Addition of synexin (0.1 microgram/ml) to an external medium lacking added calcium induced no measurable changes in either bilayer resistance (10-30 G omega) or displacement current across the membrane. However, addition of calcium (0.1-2.5 mM) in the presence of synexin in the external solution caused a marked increase in the size and time constant of decay of the displacement current. From the steady-state value of the current we calculated a 5-fold decrease in resistance and from the charge displaced during the voltage-clamp pulses we calculated a 10-fold increase in membrane capacitance (from 20 to 200 fF). The size of the synexin-specific charge displacement in one direction during a pulse was always equal to the charge returning to the original configuration after the pulse. The synexin-specific transfer of charge reached saturation when the pipet potential was taken to a sufficient positive or negative value. These properties of the extra charge movement support our view that in the presence of calcium the cytosolic protein synexin penetrates into the bilayer. It is possible that these properties may be related to the mechanism by which synexin promotes membrane fusion in natural membranes.
D017310:Annexin A7; D002118:Calcium / Q000494:pharmacology*; D004553:Electric Conductivity; D008051:Lipid Bilayers / Q000378:metabolism*; D008561:Membrane Fusion / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D010718:Phosphatidylserines / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*
D017310:Annexin A7; D008051:Lipid Bilayers; D010718:Phosphatidylserines; D011506:Proteins; D002118:Calcium