Primary rat cardiac cell culture: diet of the mother rats as a determinant parameter of cardiomyoblast production from neonates.
Two populations of Wistar rats from the same strain were fed two commonly used commercial chows. The yields of myocardial cells for primary culture obtained from neonatal rats in these 2 populations were significantly different. This effect is strickly caused by diet used during pregnancy and is quickly reversible from one pregnancy to the following one in the same females. This diet effect is limited to the yield in cardiomyoblasts and cultures were not found different after 5 days in the same medium.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004032:Diet; D005260:Female; D008431:Maternal-Fetal Exchange; D009206:Myocardium / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains