Effects of ethanol on human monocyte IgG Fc receptors.
The IgG Fc receptor function of human monocytes (Mo) exposed to ethanol in vitro was assessed by a rosette assay in which IgG-coated sheep red blood cells were used as test particles. Pre-incubation of Mo in autologous serum with ethanol (55 mM and above) for 15 min at 37 degrees C, caused a significantly lower percentage of Mo-forming rosettes. This reduction by ethanol was not observed when Mo were preincubated with ethanol in fetal calf serum or in serum-free conditions, but was present when Mo were pre-incubated with ethanol in serum-free media to which IgG (10-150 micrograms/ml) had been added. Donor-dependent differences were observed in the reduction of Mo-forming rosettes in the presence of autologous serum and ethanol. Mo allowed to form rosettes without ethanol were exposed to ethanol during a subsequent phagocytosis process. A dose-dependent inhibition of the internalization of test particles was found in Mo from all donors.
D002478:Cells, Cultured; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects; D011961:Receptors, Fc / Q000187:drug effects*; D017452:Receptors, IgG; D012397:Rosette Formation
D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG; D000431:Ethanol