[Hypoglycemic effect of insulin incorporated into liposomes after oral administration to animals with different types of experimental diabetes].
Different doses of insulin incorporated into liposomes were administered to normal animals and to those with certain forms of experimental diabetes. Lecithin-cholesterol liposomes in the molar ratio 9:1 were used. They were formed by the supersound treatment of the lipid suspension with the crystalline insulin in the buffer containing 140 mM NaCl and 10 mM tris-HCl (pH 7.4). Incorporation of insulin into liposomes was 16.2% in determination by [125I] insulin and 9.7% in determination of immunoreactive insulin after destruction of liposomes. Dynamics of glycemia and insulinemia was studied in these animals. It is established that insulin from liposomes being per os administered to animals evokes an expressed hypoglycemic effect and hyperinsulinemia. Effective sugar-lowering doses of liposomal insulin for animals with the experimental diabetes were from 6 ME/kg and for normal animals--from 30 ME/kg.
D000284:Administration, Oral; D000496:Alloxan; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D007328:Insulin / Q000008:administration & dosage* / Q000097:blood / Q000627:therapeutic use; D008081:Liposomes / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013311:Streptozocin
D001786:Blood Glucose; D007328:Insulin; D008081:Liposomes; D013311:Streptozocin; D000496:Alloxan
Gipoglikemicheskiĭ éffekt insulina, vkliuchennogo v liposomy, pri oral'nom vvedenii zhivotnym s razlichnymi tipami éksperimental'nogo diabeta.