Effects of cyproterone acetate and flutamide on the testis and epididymis of the Indian wall lizard, Hemidactylus flaviviridis (Ruppell).
Intramuscular injections of 0.2 mg cyproterone acetate (CA) or flutamide every other day for 6 weeks resulted in the inhibition of spermatogenesis. While CA treatment reduced the weight of the testis significantly, flutamide did not. Inhibition of steroidogenesis, indicated by an accumulation of sudanophilic lipid and a decrease in delta 5-3 beta-hydroxysteroid dehydrogenase and glucose-6-phosphate dehydrogenase activity, was evident in the Leydig cells of CA-treated testis. Flutamide, on the other hand, had no effect on the activity of Leydig cells. A marked decline in epididymal weight, as well as reduction in epithelial cell height, was caused by both CA and flutamide. The epithelial cells of epididymes of treated lizards exhibited an accumulation of sudanophilic lipid material in their cytoplasm. However, sudanophilic secretions present in the lumina of epididymal tubules were greatly reduced. This indicates either lack of synthesis of lipid or decrease in its turn over. Our results are in agreement with those obtained in mammalian species after CA or flutamide treatment where a decrease in fertility is suggested.
D000813:Anilides / Q000494:pharmacology*; D000818:Animals; D003534:Cyproterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D017373:Cyproterone Acetate; D004822:Epididymis / Q000166:cytology / Q000187:drug effects*; D004847:Epithelial Cells; D005485:Flutamide / Q000494:pharmacology*; D007985:Leydig Cells / Q000166:cytology; D008116:Lizards / Q000502:physiology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000166:cytology / Q000187:drug effects*
D000813:Anilides; D017373:Cyproterone Acetate; D005485:Flutamide; D003534:Cyproterone