title: Endocytosis of aggregated immunoglobulin G by rat basophilic leukemia cells; rate, extent, and effects on the endocytosis of immunoglobulin E.
abstract: Rat basophilic leukemia (RBL) cells have distinct receptors for IgE and IgG. We assessed the endocytosis of chemically and immunochemically cross-linked mouse-IgG and its influence on the simultaneous endocytosis of IgE. We found that at 37 degrees C, aggregates of IgG and IgE were endocytosed at about the same rate with one-half of the maximal endocytosis occurring in 5 to 13 min, and the efficiency of endocytosis for both ligands ranging from 40 to 70%. We also found that endocytosis of cross-linked IgE and IgG occurred simultaneously and neither ligand significantly affected the rate or extent of endocytosis of the other. The cells accumulated the cross-linked IgG, and then released it to the extracellular environment, at a rate (less than 3%/hr) slower than the released endocytosed IgE (greater than 10%/hr). Using an assay that discriminates between unbound and receptor-bound oligomeric IgG, we found that oligomeric IgG is endocytosed with its receptor, and that the bulk of the ligand remains bound to its receptor for greater than 120 min after endocytosis. The differences in the rate of release of endocytosed IgG vs IgE suggests that the intracellular fate or pathway of these two oligomeric ligands may differ.
mesh_terms: D000818:Animals; D001491:Basophils / Q000378:metabolism / Q000502:physiology*; D001666:Binding Sites, Antibody; D003432:Cross-Linking Reagents; D004140:Dinitrophenols / Q000276:immunology; D004705:Endocytosis; D007073:Immunoglobulin E / Q000502:physiology*; D007074:Immunoglobulin G / Q000378:metabolism* / Q000502:physiology; D007700:Kinetics; D007938:Leukemia / Q000276:immunology / Q000378:metabolism*; D046911:Macromolecular Substances; D051379:Mice; D051381:Rats; D011961:Receptors, Fc / Q000378:metabolism / Q000502:physiology; D017452:Receptors, IgG
chemical_list: D003432:Cross-Linking Reagents; D004140:Dinitrophenols; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D011961:Receptors, Fc; D017452:Receptors, IgG; D007073:Immunoglobulin E