title: Anisakis simplex: uncoupling of oxidative phosphorylation in the muscle mitochondria of infected fish.
abstract: Adenosine triphosphatase activity stimulated by Mg2+ was greater in muscle mitochondria of fish infected with larval Anisakis simplex nematodes than in uninfected fish. When muscle mitochondria were isolated in a sucrose ethylene-glycol bis(beta-aminoethyl ether)N,N'-tetraacetic acid medium from fresh uninfected fish, they were loosely coupled, and their adenosine triphosphatase activity was comparable to that of mitochondria from rat tissue. Activity in infected fish was dose dependent, increasing with the number of worms per fish. Excretory secretory products or a cytoplasmic fraction of anisakines, when incubated with coupled rat mitochondria, also caused adenosine triphosphatase activity to increase. Storage of fish flesh caused an increase in adenosine triphosphatase activity, but such aging was not significant until 5 and 10 days after death in refrigerated and frozen samples, respectively. The Mg2+ stimulated adenosine triphosphatase activity of muscle mitochondria can be used to estimate the number of nematodes per market fish. The type of medium used to isolate the mitochondria is crucial in such studies; an ionic medium with Nagarse proteinase was optimal for fish muscle mitochondria.
mesh_terms: D019297:2,4-Dinitrophenol; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D006054:Goldfish; D007700:Kinetics; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism*; D009349:Nematode Infections / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D012474:Salmon; D013045:Species Specificity; D014337:Trout
chemical_list: D004140:Dinitrophenols; D009840:Oligomycins; D017301:Ca(2+) Mg(2+)-ATPase; D019297:2,4-Dinitrophenol