Isolation of a cDNA probe for a human jejunal brush-border hydrolase, sucrase-isomaltase, and assignment of the gene locus to chromosome 3.
We report the nucleotide sequence and derived amino acid sequence of a cDNA clone encoding most of the N-terminal, isomaltase region of human sucrase-isomaltase (SI). A plasmid containing this cDNA, pS12, identifies a 6-kb mRNA found in human jejunum and the human colon carcinoma cell line Caco-2. This human SI cDNA shows extensive overall homology with recently published rabbit SI cDNA. Using pS12 to probe DNA from a panel of somatic cell hybrids, we have assigned the gene encoding human SI to chromosome 3.
D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002460:Cell Line; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006801:Humans; D007583:Jejunum / Q000201:enzymology*; D008871:Microvilli / Q000201:enzymology*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013394:Sucrase-Isomaltase Complex / Q000235:genetics*; D014158:Transcription, Genetic
D009097:Multienzyme Complexes; D012333:RNA, Messenger; D004247:DNA; D013394:Sucrase-Isomaltase Complex