title: The J gene of phi X174: isolation and characterization of a J gene mutant.
abstract: The J gene protein of bacteriophage phi X174 is a component of the mature phage. The previous lack of J gene mutants has prevented an in vivo analysis of J protein functions. A phi X174 mutant was constructed by inserting an 11 nucleotide sequence into the J gene. This mutant, designated insJ, was viable only in the presence of a wild-type J gene carried on a plasmid that could provide J protein. An analysis of DNA synthesis during insJ mutant infection under non-permissive conditions confirmed that the J protein is not required for viral DNA synthesis.
mesh_terms: D000595:Amino Acid Sequence; D010584:Bacteriophage phi X 174 / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000096:biosynthesis; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins