Cell surface T3 expression requires the presence of both alpha- and beta-chains of the T cell receptor.
We have identified a surface T3- Jurkat variant which has a defective alpha-chain but which possesses an intact beta-chain. The transfection of a functional mouse alpha-chain into this human T cell induces the expression of surface T3 molecules associated with mouse alpha-human beta-heterodimers detected by anticlonotypic antibodies. Treatment of the transfectant with anti-T3, anti-mouse Ti-alpha, or anti-human Ti-beta antibodies clears all Ti-T3 complexes from the surface. These results demonstrate that functional alpha- and beta-chains are both required for expression of T3 on the cell membrane, and that the Ti heterodimers present and associated with T3 on Jurkat cells involve only alpha- and beta-chains.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000502:physiology*; D017252:CD3 Complex; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D004705:Endocytosis; D005434:Flow Cytometry; D006801:Humans; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D017252:CD3 Complex; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell