Immunoglobulin E-dependent stimulation of human alveolar macrophages: significance in type 1 hypersensitivity.
Human alveolar macrophages were obtained during diagnostic bronchoalveolar lavage. Cells were cultured, and morphological examination (including electron microscopy) revealed that not more than 5% of the cultured cells were identifiable as cells other than alveolar macrophages. The cells were sensitized with human myeloma immunoglobulin E. and then challenged with anti-immunoglobulin E anti-sera. The experiments employed a highly specific monoclonal antibody and three affinity purified reagents. The formation of immunoglobulin E/anti-immunoglobulin E complexes facilitated release from alveolar macrophages of leukotriene B4, prostaglandin F2 alpha, thromboxane B2 and the lysosomal hydrolase N-acetyl-beta-D-glucosaminidase. There was no release of active oxygen species, with this stimulus, as measured by lucigenin chemiluminescence. Immunoglobulin E receptors were identified histochemically on the surface of human alveolar macrophages, and were visualized as conjugates with colloidal gold by electron microscopy. These results support the view that human alveolar macrophages may contribute to type 1 hypersensitivity reactions in the lung.
D001249:Asthma / Q000276:immunology*; D006801:Humans; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000648:ultrastructure; D010100:Oxygen / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000276:immunology*; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000032:analysis*; D013929:Thromboxane B2 / Q000096:biosynthesis
D011961:Receptors, Fc; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D013929:Thromboxane B2; D010100:Oxygen