Interaction of benoxaprofen with rat erythrocytes: effects on oxidative metabolism and membrane ATPase activities.
Incubation of rat erythrocytes with benoxaprofen resulted in increased glucose utilization, lactate production, depletion of cellular ATP and significant haemolysis. In isolated red cell membranes, benoxaprofen produced a dose related stimulation of both Ca2+ dependent and Ca2+ independent ATPase activities. Coincubation of either erythrocytes or red cell membranes with cysteine ameliorated the effects of benoxaprofen. A possible association exists between the mechanism of the reported anti-inflammatory activity of benoxaprofen and its cellular toxicity.
D000251:Adenosine Triphosphatases / Q000097:blood*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D003545:Cysteine / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D011422:Propionates / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011422:Propionates; C011677:benoxaprofen; D000251:Adenosine Triphosphatases; D003545:Cysteine