The induction of helper and suppressor cells with secondary anti-hen egg-white lysozyme B hybridoma cells in the absence of antigen.
The results presented in this report define a dominant T cell-recognized public idiotype (SRId) expressed on monoclonal anti-chicken egg-white lysozyme (HEL) antibodies produced by hybridomas derived from secondary response lymphocytes. This Id mediates interactions between SRId+ B cells and SRId-recognizing T cells. In the absence of exogenous antigen, irradiated secondary anti-HEL B hybridoma cells (B-Hyb) of nonoverlapping specificity can be used to induce a helper T cell population capable of specifically stimulating an in vitro anti-HEL plaque-forming cell (PFC) response. Importantly, similar immunizations using carbodiimide-treated secondary anti-HEL B-Hyb cross-primed for a suppressor T cell population capable of suppressing this in vitro anti-HEL PFC response. That is, suppression was seen not only to the response induced by the homologous B-Hyb but to other B-Hyb which express anti-HEL monoclonal antibody of nonoverlapping specificity. This evidence is consistent with the presence of a pre-existent regulatory Id network involving SRId in antigennaive animals. After immunization with HEL, regulatory cells exert a strong selective pressure which leads to a secondary anti-HEL B population, of varying fine specificity, but uniformly positive for SRId.
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002234:Carbodiimides; D006183:H-2 Antigens / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D007108:Immune Tolerance; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007156:Immunologic Memory; D051379:Mice; D009113:Muramidase / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013997:Time Factors
D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D002234:Carbodiimides; D006183:H-2 Antigens; D007130:Immunoglobulin Idiotypes; D009113:Muramidase