title: Correlation between hysteresis and allosteric properties for phosphofructokinase from Ascaris suum.
abstract: The Ascaris suum phosphofructokinase exhibits hysteretic transitions in the time course for fructose 6-phosphate (F6P) phosphorylation in addition to allosteric properties when assayed at pH values below 8. Conditions that enhance hysteretic changes also enhance cooperative interactions and thus there appears to be a link between hysteresis and cooperativity. Initiation of reaction with either F6P or phosphofructokinase results in a pronounced lag, while initiation of the reaction with MgATP results in a burst at pH values below 8. Under conditions in which a lag is evident, increasing the concentration of F6P in the assay decreases the lag, while under conditions where a burst is evident, increasing the concentration of MgATP in the assay decreases the burst. The lag is enzyme-dependent going to a limiting value at high enzyme concentration, while the burst is enzyme-independent. As the pH increases, the Hill coefficient for F6P decreases from a pH-independent value of 3 at low pH to a value of 1 above pH 8. Over the same pH range, the burst rate increases to a point that it is too fast to measure at pH 8 (that is, the time course is linear). Finally, at pH 6.9, the saturation curve for F6P becomes more cooperative with the Hill coefficient equal to 3 above 4 mM MgATP. Data are interpreted in terms of the model suggested for the rabbit skeletal muscle phosphofructokinase (Frieden, C., Gilbert, H. R., and Bock, P.E. (1976) J. Biol. Chem. 251, 5644-5647) in which MgATP binds preferably to an inactive tetrameric enzyme form in which a group with a pK of 6.8 is protonated and F6P binds preferably to the unprotonated active tetrameric form.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000494:Allosteric Regulation; D000818:Animals; D001200:Ascaris / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D010766:Phosphorylation; D013997:Time Factors
chemical_list: D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1