A receptor-antibody sandwich assay for teicoplanin.
We have developed a new method for quantifying teicoplanin in complex matrixes, a receptor-antibody sandwich assay (RASA). The method is based on bioselective adsorption of teicoplanin onto microtiter plates coated with albumin-epsilon-aminocaproyl-D-alanyl-D-alanine, a synthetic analog of its biological target, and reaction with anti-teicoplanin antibodies. The sandwich complexes are detected by incubation with peroxidase-labeled goat antibodies to rabbit IgGs and chromogenic reaction with o-phenylenediamine. The dose-response curve was linear for teicoplanin concentrations in the range from 0 to 0.15 mg/L. We used the assay to measure teicoplanin concentrations in various biological matrixes. Analytical recovery from serum was 99.5%, the interassay CV was 5.1%, and the detection limit was 30 micrograms/L (P less than 0.01). Mean analytical recoveries from other biological specimens were 98% from ascitic fluid, 100% from pleuric liquid, 104.8% from prostate homogenate, and 98.5% from bronchial expectorate.
D000900:Anti-Bacterial Agents / Q000032:analysis*; D000906:Antibodies; D005122:Exudates and Transudates / Q000032:analysis*; D006020:Glycopeptides / Q000032:analysis; D006801:Humans; D007158:Immunologic Techniques; D007164:Immunosorbents; D011786:Quality Control; D017334:Teicoplanin; D014640:Vancomycin / Q000032:analysis
D000900:Anti-Bacterial Agents; D000906:Antibodies; D006020:Glycopeptides; D007164:Immunosorbents; D017334:Teicoplanin; D014640:Vancomycin