[Studies on the antitumor activity of human recombinant interferon alpha-2b in vitro].
Correlation between antiproliferative and binding activities of interferon (IFN) alpha-2b to various human cell lines was examined using human recombinant IFN alpha-2b. Burkitt's lymphoma Daudi cells and human renal cell carcinoma OS-RC-2 cells were sensitive to IFN alpha-2b, whereas two EB-virus-transformed B cell lines, FS and L-KT3, and human A375 melanoma cells showed low or no sensitivity. 125I-IFN alpha-2b binding assay revealed that the difference in IFN alpha-2b sensitivity was related to the number and the affinity of IFN alpha-2b receptors per cell. Experiments were then performed to investigate the influence of recombinant IFN alpha-2b on the cytostatic activity of monocytes against A375 cells in vitro. IFN alpha-2b enhanced the cytostatic activity of monocytes against A375 cells which showed low sensitivity to the direct growth inhibitory effect of IFN alpha-2b. Depletion of NK cells from the monocyte preparations by anti-Leu-11b monoclonal antibody and complement did not affect the monocyte activation by IFN alpha-2b, indicating that NK cells were not involved in this system.
D002051:Burkitt Lymphoma / Q000276:immunology; D002292:Carcinoma, Renal Cell / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D007680:Kidney Neoplasms / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology; D011971:Receptors, Immunologic / Q000378:metabolism; D017471:Receptors, Interferon
D007370:Interferon Type I; D011971:Receptors, Immunologic; D017471:Receptors, Interferon