Regional assignment of human liver-type 6-phosphofructokinase to chromosome 21q22.3 by using somatic cell hybrids and a monoclonal anti-L antibody.
The three structural loci encoding human phosphofructokinase, a key regulatory enzyme of glycolysis, are located on separate chromosomes. The gene coding for the liver-type subunit PFKL has previously been assigned to chromosome 21. We have used a subunit- and human-specific monoclonal antibody to liver PFK to detect the expression of human PFKL in hamster X human hybrid cell lines. A cell line carrying an 8;21 translocation which contains all of chromosome 21 except the band 21q22.3 was negative for the expression of PFKL whereas cell lines carrying the reciprocal 8;21 translocation were positive. In addition, a cell line with a ring chromosome 21 containing a breakpoint which excluded the distal part of the q22.3 band was negative for expression of PFKL. These results indicate that human PFKL is located on chromosome 21q22.3.
D000818:Animals; D000911:Antibodies, Monoclonal; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008099:Liver / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000235:genetics*
D000911:Antibodies, Monoclonal; D010732:Phosphofructokinase-1