Histologic study of the inhibition of bone resorption in organ cultures by myo-inositol-2-monophosphate.
This study examines chemically and histologically the relative abilities of inositol monophosphate (IP1), inorganic phosphate (Pi), ethane-1-hydroxy-1, 1-diphosphonate (EHDP) and dichloromethylene disphosphonate (Cl2MDP) to inhibit parathyroid hormone (PTH)--induced resorption of fetal rat long bones in organ culture. Pregnant rats injected with 45Ca on the 18th day of gestation were killed the next day and their fetuses removed. Half of each pair of dissected long bones was incubated in a chemically defined control medium while the contralateral half was incubated in medium containing PTH or PTH plus the compound to be tested. 45Ca released into the medium was indicative of the amount of bone resorption. Bones were then processed histologically and examined microscopically. All compounds inhibited resorption to some extent with IP1 and Pi being less effective than EHDP or C12MDP at comparable phosphate concentrations. However, the disphosphonates damaged osteoclasts whereas IP1 and Pi did not. This suggests that IP1 may inhibit resorption by a different mechanism perhaps related only to prevention of crystal dissolution.
D000818:Animals; D001862:Bone Resorption / Q000187:drug effects* / Q000473:pathology; D001842:Bone and Bones / Q000033:anatomy & histology / Q000187:drug effects; D004002:Clodronic Acid / Q000008:administration & dosage / Q000494:pharmacology; D012968:Etidronic Acid / Q000008:administration & dosage / Q000494:pharmacology; D005333:Fetus; D007295:Inositol Phosphates / Q000008:administration & dosage / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010010:Osteoclasts / Q000187:drug effects / Q000648:ultrastructure; D010281:Parathyroid Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D013403:Sugar Phosphates / Q000494:pharmacology*
D007295:Inositol Phosphates; D010281:Parathyroid Hormone; D013403:Sugar Phosphates; D004002:Clodronic Acid; C047872:inositol 2-monophosphate; D012968:Etidronic Acid