title: Double-inhibitor and uncoupler-inhibitor titrations. 2. Analysis with a nonlinear model of chemiosmotic energy coupling.
abstract: The results of double-inhibitor and uncoupler-inhibitor titrations have been simulated and analyzed with a nonlinear model of delocalized protonic coupling obtained by linking two proton pump models of the kind studied by Pietrobon and Caplan [Pietrobon, D., & Caplan, S. R. (1985) Biochemistry 24, 5764-5776] through their common intermediate delta mu H. It is shown that the results predicted by a delocalized chemiosmotic model are highly dependent on the kind of relationships existing between rate of ATP synthesis, Jp, and delta mu H and rate of electron transfer, Je, and delta mu H. With nonlinear flow-force relationships all the results reported so far are not necessarily inconsistent with the delocalized chemiosmotic model provided that the relationships between rates and delta mu H satisfy the following requirements: Jp/delta mu H increases and/or Je/delta mu H decreases as (delta mu H) increases.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological
chemical_list: D000251:Adenosine Triphosphatases