Inhibition of growth of HeLa and WI-38 cells by dehydroepiandrosterone and its reversal by ribo- and deoxyribonucleosides.
Dehydroepiandrosterone (DHEA), an adrenal steroid of no known biological function, is a potent inhibitor of mammalian glucose-6-phosphate dehydrogenase (G6PDH). DHEA inhibited the growth of two stains of HeLa and WI-38 cells in culture. One of the HeLa strains, TCRC-2, was about 10x as sensitive to growth inhibition as the two other cell lines. The G6PDH activity in cell extracts of HeLa TCRC-2 was also much more sensitive to DHEA inhibition than the G6PDH activities of the other cell lines. The addition of a combination of four deoxyribonucleosides and four ribonucleosides to the culture medium overcame the DHEA-induced growth inhibition in the HeLa TCRC-2 line.
D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D003853:Deoxyribonucleosides / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006367:HeLa Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000196:embryology; D012263:Ribonucleosides / Q000494:pharmacology*
D003853:Deoxyribonucleosides; D012263:Ribonucleosides; D003687:Dehydroepiandrosterone; D005954:Glucosephosphate Dehydrogenase