In vitro effects of T-2 toxin on the mitogen responsiveness and antibody-producing ability of human lymphocytes.
The effects of T-2 toxin on the in vitro mitogen responses and the antibody-producing ability of human peripheral blood lymphocytes were evaluated. T-2 toxin inhibited the mitogen response to concanavalin A (ConA) at a lower concentration (1.6 ng/ml) as compared to phytohemagglutinin (2.4 ng/ml) and pokeweed mitogen (2.4 ng/ml). Maximum inhibition was observed when the toxin was present during the first 8 h; however, the cultures were not refractory to inhibition until 48 h after culture initiation. The antibody-producing ability was inhibited by T-2 toxin concentrations of greater than or equal to 3.2 ng/ml. T-2 toxin did not induce or interfere with the generation of suppressor cells by ConA. The results of this study indicate that various lymphocyte subpopulations have different susceptibilities to T-2 toxin. The activation process associated with lymphocyte proliferation appears to be one of the most sensitive time periods.
D000917:Antibody Formation / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007958:Leukocyte Count / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012717:Sesquiterpenes / Q000633:toxicity*; D013605:T-2 Toxin / Q000633:toxicity*
D010835:Phytohemagglutinins; D012717:Sesquiterpenes; D003208:Concanavalin A; D013605:T-2 Toxin