Distances between the FITC-binding site in the (Ca2++Mg2+)-ATPase from sarcoplasmic reticulum and fluorescent probes located in the membrane.
Fluorescence energy transfer measurements have been performed to investigate the distances between the FITC-binding site in the (Ca2++Mg2+)-ATPase from sarcoplasmic reticulum and 8-aniline-1-naftalenesulfonate (ANS-) and diphenylhexatriene (DPH) localized in different sites of the membrane. The distances calculated between FITC bound to the ATPase and ANS- and DPH in the membrane, were approx. 51 A and 60 A, respectively.
D000817:Anilino Naphthalenesulfonates / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D011485:Protein Binding; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D013050:Spectrometry, Fluorescence; D013861:Thiocyanates / Q000378:metabolism*
D000817:Anilino Naphthalenesulfonates; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; C027132:1-anilino-8-naphthalenesulfonate; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D016650:Fluorescein-5-isothiocyanate