Influence of inhibitors of poly(ADP-ribose)polymerase activity on X-ray and ultraviolet light induced killing and mutation in Chinese hamster V79 cells.
The effect of nicotinamide or benzamide, inhibitors of poly(ADP-ribose)polymerase activity, on X-ray and ultraviolet light induced killing of asynchronously dividing V79 Chinese hamster cells was to reduce the shoulder width of the survival curve; the rate of killing remaining unchanged and the yield of 8-azaguanine resistant mutants also remaining unaffected. However, when density inhibited cells were irradiated with X-rays or u.v. light, holding at this density allowed potentially lethal damage recovery (PLDR) to occur, enhancing survival. Inhibitors of poly(ADP-ribose)polymerase suppressed PLDR and also increased the yield of mutants. Thus the role of poly(ADP-ribose) in X-ray or u.v. induced killing and mutation was dependent on the condition of growth.
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002470:Cell Survival / Q000528:radiation effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis; D009154:Mutation; D009536:Niacinamide / Q000494:pharmacology; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000502:physiology; D014357:Trypsin / Q000494:pharmacology; D014466:Ultraviolet Rays; D014965:X-Rays
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D009536:Niacinamide; C037689:benzamide; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D014357:Trypsin