Mechanisms of immunological response induced in CD2F1 mice by administration of semisyngeneic L 1210 leukemia cells treated with cyclophosphamide.
CD2F1 mice were immunized against semisyngeneic L 1210 leukemia. Immunization was achieved by four i.p. injections, in weekly intervals, of L 1210 cells treated in vivo twice with 200 mg/kg of cyclophosphamide. The immunized animals survived i.p. challenge with 1000 untreated L 1210 cells that was lethal for nonimmunized mice. The immunity could be abrogated in vivo with anti-mouse thymocyte serum, carrageenan or reserpine, but not by anti-mouse IgG serum, suggesting participation of T lymphocytes and macrophages in the response. Moreover, lymphocytes and macrophages from the peritoneal cavity of immunized mice were cytotoxic in vitro for L 1210 cells. The immunity, at least partially, could be adoptively transferred with peritoneal exudate cells or splenocytes.
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D003348:Cortisone / Q000494:pharmacology; D003520:Cyclophosphamide / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D007111:Immunity, Cellular / Q000187:drug effects*; D007114:Immunization; D007116:Immunization, Passive; D007939:Leukemia L1210 / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D012110:Reserpine / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D012110:Reserpine; D003520:Cyclophosphamide; D002351:Carrageenan; D003348:Cortisone