Wheat germ agglutinin induces compaction- and cavitation-like events in two-cell mouse embryos.
The lectin wheat germ agglutinin (WGA) has been observed to induce morphological events similar to compaction and cavitation in 2-cell mouse embryos. In vitro exposure of embryos to WGA results in increased apposition between blastomeres and the subsequent formation of a large intercellular cavity. As is the case for cavitation normally associated with blastocyst formation, WGA-induced cavitation can be inhibited by ouabain, suggesting a requirement for ATPase activity. However, WGA-induced effects are not inhibited by cytoskeletal disruptive agents or inhibitors of a variety of synthetic and metabolic functions. WGA may induce the observed effects by triggering the premature onset of developmental events normally involved in the processes of compaction and cavitation or, perhaps, by inducing morphologically similar changes as a result of the crosslinking of cell surface lectin-binding molecules and regional inhibition of ATPase function.
D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D001757:Blastomeres / Q000187:drug effects* / Q000502:physiology; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D005260:Female; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR / Q000196:embryology; D008856:Microscopy, Fluorescence; D011817:Rabbits; D013997:Time Factors; D014909:Wheat Germ Agglutinins
D037102:Lectins; D014909:Wheat Germ Agglutinins; D017301:Ca(2+) Mg(2+)-ATPase