A unique four-stranded model of a homologous recombination intermediate.
This paper proposes a model of four-stranded DNA synapsis during recombination between homologous segments of two DNA duplexes. The proposed intermediate is one of only two known models having relative chain orientations about the synaptic junction that are consistent with recent topological results on the integrative recombination of bacteriophage lambda. This model has the advantage of providing a mechanism for recognition of sequence homology between duplexes through specific hydrogen-bond formation; other models are discussed in comparison. The new model is based on an alternative family of DNA structures having chain directions opposite to those of the Watson-Crick family of structures. Idealized coordinates for generating both right- and left-handed forms of these alternative structures are presented for further study.
D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral; D006860:Hydrogen Bonding; D008242:Lysogeny; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral