[Anionic detergents, natural and synthetic, as selective denaturants of various dehydrogenases].
The effects of natural (fatty acids) or synthetic detergents on some highly purified enzymes has been studied. Four dehydrogenases (glucose-6-phosphate-, lactico-, malico- and isocitrico-dehydrogenase) are highly inhibited by these products. Other enzymes are not inhibited or inactivated by them. The mechanism of the action of detergents is specifically based on their binding to the enzyme at the level of its proteic constituent and close to its active group.
D000818:Animals; D003902:Detergents / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors*; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors*; D008291:Malate Dehydrogenase / Q000037:antagonists & inhibitors*; D008968:Molecular Conformation; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009243:NAD; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000494:pharmacology*; D013552:Swine
D003902:Detergents; D005227:Fatty Acids; D013501:Surface-Active Agents; D009243:NAD; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase
Les détergents anioniques, naturels et de synthèse, dénaturants sélectifs de certaines déshydrogénases.