Protective effect of S-adenosylmethionine against the induction of chromosome fragile sites.
Chromosome fragile sites were induced in the peripheral lymphocytes of 7 donors by treatment of the cells in culture with 5-fluorodeoxyuridine and caffeine. The appearance of these fragile sites was inhibited to a large extent and in a dose-dependent manner by S-adenosylmethionine. It is possible that this methyl donor exerted its protective action through restoration of the correct level of methylation of the DNA.
D002110:Caffeine / Q000494:pharmacology; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility / Q000187:drug effects*; D004249:DNA Damage; D005467:Floxuridine / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008745:Methylation; D012436:S-Adenosylmethionine / Q000494:pharmacology*
D005467:Floxuridine; D002110:Caffeine; D012436:S-Adenosylmethionine