Determination of (E)-1,2,3,4-tetrahydro-1,1,4,4-tetramethyl-6-(1-methyl-2- phenylethenyl) naphthalene, an antiacne agent, and its phenolic metabolite in plasma by reversed-phase high-performance liquid chromatography.
A rapid, sensitive and selective high-performance liquid chromatographic (HPLC) assay was developed for the determination of (E)-1,2,3,4-tetrahydro-1,1,4,4-tetramethyl-6-(1-methyl-2- phenylethenyl)naphthalene (I) and its phenolic metabolite, (E)-4-[2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-2- methylethenyl]phenol (II) in plasma. The assay for both compounds involves precipitation of the plasma proteins with acetonitrile, followed by extraction of the entire mixture into methyl tert.-butyl ether and subsequent analysis by reversed-phase HPLC. The overall recovery of I and II was 96.9 +/- 5.3 and 96.2 +/- 5.8% for dog plasma, 80.0 +/- 4.0 and 93.5 +/- 5.0% for human plasma and 97.4 +/- 2.9 and 97.6 +/- 9.6% for rat plasma, respectively. The sensitivity limit is 20 ng/ml of plasma for both compounds I and II using UV detection at 280 nm. The HPLC assay was used in studies in the dog and in the rat.
D000152:Acne Vulgaris / Q000188:drug therapy*; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000097:blood*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004355:Drug Stability; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D009281:Naphthalenes / Q000097:blood*; D051381:Rats; D012176:Retinoids; D012737:Sex Factors; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013764:Tetrahydronaphthalenes / Q000097:blood*
D000891:Anti-Infective Agents, Local; D007202:Indicators and Reagents; D009281:Naphthalenes; D012176:Retinoids; D013764:Tetrahydronaphthalenes; C053595:temarotene