Isoenzyme studies of Giardia lamblia isolated from symptomatic cases.
Strains of Giardia lamblia were isolated from symptomatic cases of giardiasis and axenized in the laboratory. Electrophoretic mobility patterns of four enzymes, viz., EC 5.3.1.9 glucose phosphate isomerase (GPI); EC 1.1.1,4.0.L-malate; NADP+ Oxidoreductase (Oxaloacetate decarboxylating) (ME); EC 2.7.5.1 phosphoglucomutase (PGM); and EC 2.7.1.1 hexokinase (HK) of the lysates prepared from these isolates were studied using starch-gel. Based on differences in mobility patterns of PGM and HK, the four strains studied could be grouped into three different isoenzyme types (Zymodemes). ME mobility was identical in all the four strains. Some relative difference was seen in the mobility of GPI, though the pattern of mobility was similar in all the strains.
D000293:Adolescent; D000818:Animals; D002648:Child; D004386:Duodenum / Q000469:parasitology; D004592:Electrophoresis, Starch Gel; D005260:Female; D005872:Giardia / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D005873:Giardiasis / Q000469:parasitology*; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010733:Phosphoglucomutase / Q000378:metabolism
D007527:Isoenzymes; D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); D006593:Hexokinase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase