[Primary cultures of epithelial cells and long-term cultures of fibroblasts from the human umbilical cord: ultrastructural and immunocytochemical characterization].
Repeated trypsinization of the full term human umbilical cord epithelium allows an homogeneous and exclusively epithelial primary culture, without fibroblastic growth. Transmission electron microscopy observations of desmosomes, cytokeratin intermediate filaments as revealed by indirect immunofluorescence and cultural aspects confirm the epithelial nature of this primary culture. Fibroblasts obtained by an explants culture method exhibit neither desmosomes nor cytokeratin intermediate filaments, which are epithelial markers. They yield characteristic long term cultures of fibroblastic aspect and growth.
D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D008854:Microscopy, Electron; D014470:Umbilical Cord / Q000166:cytology* / Q000648:ultrastructure
Cultures primaires de cellules épithéliales et cultures à long terme de fibroblastes à partir du cordon ombilical humain: caractérisation ultrastructurale et immunocytochimique.