Identification of the plasma membrane receptor for interleukin-1 on mouse thymoma cells.
The plasma membrane receptor for interleukin-1 (IL-1) has been characterized from mouse EL4-6.1 thymoma cells. Following binding of IL-1 to surface labeled EL4-6.1 cells, the IL-1 binding molecule was immuno-precipitated using a rabbit antiserum against the hormone. The putative IL-1 receptor is a membrane-associated glycopeptide of Mr = 82,000 containing probably two or three N-linked glycan units as indicated by its conversion into a Mr = 60,000 polypeptide upon deglycosylation with endo-beta-N-glycosidase F.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D007375:Interleukin-1 / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D011971:Receptors, Immunologic / Q000302:isolation & purification / Q000378:metabolism*; D017472:Receptors, Interleukin-1; D013601:T-Lymphocytes / Q000276:immunology; D013945:Thymoma / Q000276:immunology*; D013953:Thymus Neoplasms / Q000276:immunology*
D007375:Interleukin-1; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1