Drug action on articular cartilage surface. An in vitro study using mouse femoral heads labeled with cationized ferritin.
The direct effects on the cartilage articular surface of three anti-inflammatory drugs (Diclofenac, Pirprofen and acetyl-salicylic acid) and of a polysulfated glycosaminoglycan (Arteparon), were studied using an in vitro system in which BALB-c mouse femoral heads were incubated with the drugs. After incubation and labeling of the negative charges of the articular surfaces with cationized ferritin, the femoral heads were examined by electron microscopy. In addition, the effect of the drugs on the aggressive action of collagenase on the articular surface was tested using the same in vitro system. Diclofenac, Pirprofen and the polysulfated glycosaminoglycan did not alter the structure or the charge properties of the surface. Acetyl salicylic acid produced a slight disruption of the articular surface. The drugs studied had no effect on the disruptive action of collagenase.
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D004008:Diclofenac / Q000494:pharmacology; D005270:Femur Head / Q000187:drug effects; D005293:Ferritins / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010666:Phenylpropionates / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D006025:Glycosaminoglycans; D010666:Phenylpropionates; C010297:polycationic ferritin; D004008:Diclofenac; C010034:Arteparon; D005293:Ferritins; D001241:Aspirin; C008924:pirprofen