The use of Ultroser G as a serum substitute in the culture of human skin fibroblasts.
Growth of human skin fibroblasts was dramatically enhanced when serum in the culture medium was replaced by Ultroser G. Compared to the same cells cultured in the presence of serum, alterations in glucose and lipid metabolism and an increase in the activity of prolidase (EC 3.4.13.9) and prolinase (EC 3.4.13.8) were also observed. Consequently, we advise extreme caution in the use of Ultroser G in metabolic studies, especially for periods of culture exceeding 10 days. However, Ultroser G can help to produce a large number of cells and so facilitate purification of the proteins produced during the stationary growth phase.
D001205:Ascorbic Acid / Q000494:pharmacology; D001769:Blood; D001802:Blood Substitutes / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003584:Cytological Techniques; D004150:Dipeptidases / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D009930:Organic Chemicals; D012867:Skin / Q000166:cytology* / Q000201:enzymology
D001802:Blood Substitutes; D007773:Lactates; D009930:Organic Chemicals; C044609:Ultroser G; D019344:Lactic Acid; D004150:Dipeptidases; C022454:prolyl dipeptidase; C022449:proline dipeptidase; D005947:Glucose; D001205:Ascorbic Acid