Dual genotype in cutaneous T cell lymphoma: immunoglobulin gene rearrangement in clonal T cell malignancy.
Genomic DNA digests of peripheral blood lymphocytes from 13 patients with the leukemic phase of the T cell neoplasm cutaneous T cell lymphoma were studied by hydridization using probes for the constant region of the beta chain of the T cell receptor, the joining region of the immunoglobulin heavy chain gene, and the kappa and lambda light chain genes. Lymphocytes from all 13 cutaneous T cell lymphoma patients contained DNA with clonal rearrangements of the beta chain gene of the T cell receptor. In addition, DNA from 4 patients contained an immunoglobulin gene rearrangement. T cell enrichment studies of peripheral blood lymphocytes from 2 patients confirmed that the immunoglobulin gene joining region rearrangement was confined to the T cell population. These results demonstrate that cutaneous T cell lymphoma is a clonal T cell malignancy that frequently expresses a dual genotype. A multiparameter approach, including DNA probes for the beta chain of the T cell receptor, as well as the immunoglobulin genes, immunophenotyping, and cytogenetics, is valuable in the diagnosis of cutaneous T cell lymphoma.
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm / Q000235:genetics; D005803:Genes, Immunoglobulin; D005838:Genotype; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007621:Karyotyping; D008223:Lymphoma / Q000235:genetics* / Q000276:immunology; D009693:Nucleic Acid Hybridization; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D012878:Skin Neoplasms / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D007127:Immunoglobulin Constant Regions; D007144:Immunoglobulin J-Chains; D007133:Immunoglobulin Joining Region; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta