Mersalyl, a sulfhydryl reagent, alters the solubility of myosin and cytoskeletal proteins of human platelets.
We have examined the effect of a mercurial sulfhydryl reagent, mersalyl, on the protein composition of cytoskeletons by SDS-polyacrylamide gel electrophoresis after treatment of human platelets with Triton X-100 (Triton) containing mersalyl and Ca2+, and have found that mersalyl alters the protein composition of cytoskeletons in a Ca2+-dependent manner. At 1 X 10(-7) M Ca2+, 0.2 mM mersalyl, which represents approximately the equivalent amount of sulfhydryl of platelet suspensions that we used, specifically made myosin insoluble. The amount of myosin in Triton-mersalyl residues was increased by increasing the Ca2+ concentration of Triton lysis buffer. Actin-binding protein, 235 kDa polypeptide and alpha-actinin-like protein were decreased in Triton residues by mersalyl at Ca2+ concentrations less than 1 X 10(-7) M, while these polypeptides in Triton residues were increased by mersalyl in the presence of more than 2 X 10(-7) M Ca2+. Electron microscopic study revealed the presence of thick filaments with an appearance similar to that of the thick filaments of platelet myosin. Thus, the modification with mersalyl of sulfhydryls of platelet polypeptides along with changes in Ca2+ concentrations within a physiological range leads to changes in solubility of, and filament formation of, myosin, actin and other cytoskeletal proteins.
D000205:Actomyosin / Q000097:blood; D000251:Adenosine Triphosphatases / Q000097:blood; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000502:physiology; D011232:Chemical Precipitation; D003598:Cytoskeletal Proteins / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008634:Mersalyl / Q000494:pharmacology*; D008854:Microscopy, Electron; D009218:Myosins / Q000097:blood*; D017830:Octoxynol; D009941:Organomercury Compounds / Q000494:pharmacology*; D011092:Polyethylene Glycols; D012995:Solubility; D013438:Sulfhydryl Compounds / Q000502:physiology; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D003598:Cytoskeletal Proteins; D009941:Organomercury Compounds; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D011092:Polyethylene Glycols; D008634:Mersalyl; D017830:Octoxynol; D000205:Actomyosin; D000251:Adenosine Triphosphatases; D009218:Myosins; D002118:Calcium