title: Shedding of the common acute lymphoblastic leukemia antigen (CALLA) by lymphoblastoid cell lines.
abstract: The release of common acute lymphoblastic leukemia antigen (CALLA) into culture medium by lymphoblastoid cell lines was studied. CALLA release was detected using a solid phase indirect radioimmunometric assay which combines the specificity of lectins and monoclonal antibodies and using immunoadsorption of labeled CALLA in spent medium from cells incubated with 35S-methionine. No CALLA was present in the medium of cells pulse-labeled at 37 degrees C, when they were placed at 4 degrees C, thus this is an active process. Sequential analysis of soluble CALLA revealed shedding of CALLA to be associated with cell growth.
mesh_terms: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000378:metabolism*; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D003470:Culture Media; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D015260:Neprilysin; D011863:Radioimmunoassay; D012995:Solubility; D013696:Temperature; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D003470:Culture Media; D006023:Glycoproteins; D008565:Membrane Proteins; D015260:Neprilysin