Basement membrane matrix in vitro: focal binding of exogenous fibronectin to the matrix of teratocarcinoma-derived endodermal cells.
Interaction of exogenous fibronectin with the basement membrane-like PYS-2 cell matrix, lacking fibronectin and hyaluronic acid but containing heparan sulfate proteoglycan, was studied in vitro. Both human plasma fibronectin and fibronectin in fetal calf serum bound to PYS-2 matrix; also, fragments of fibronectin containing heparin-binding domains but lacking the collagen-binding domain bound to the matrix. In immunoelectron microscopy the bound fibronectin was found as 20-40 nm globules or patches. Distribution of fibronectin differed from that of laminin and correlated best with that of heparan sulfate proteoglycan. The results suggest that the binding of fibronectin to basement membrane matrices is not due to random adherence but involves specific interactions with other components.
D000818:Animals; D001485:Basement Membrane / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D002460:Cell Line; D005353:Fibronectins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006025:Glycosaminoglycans / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism*; D013724:Teratoma / Q000473:pathology*
D005353:Fibronectins; D006025:Glycosaminoglycans; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic