Localization of beta-endorphins in the rat spinal cord using avidin-biotin technology.
The avidin-biotin-peroxidase technique is proposed for the immunological localization of beta-endorphin in the rat spinal cord. A rabbit specific antibody anti-human beta-endorphin was first obtained and then identified by immunoblotting and incubated with a quick-frozen section of young rat spinal cord. The use of a specific antibody with the immunoperoxidase reaction gave a morphological visualization of the beta-endorphin in the histological sections of the rat spinal cord.
D000375:Aging / Q000378:metabolism; D000818:Animals; D001360:Avidin; D001710:Biotin; D004723:Endorphins / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000032:analysis*; D001615:beta-Endorphin
D004723:Endorphins; D001360:Avidin; D001615:beta-Endorphin; D001710:Biotin