Heat-denatured albumin-coated Dacron vascular grafts: physical characteristics and in vivo performance.
Dacron fabrics with a wide range of porosities were autoclaved for 3 minutes after being soaked in serum, 5% albumin, or 25% albumin. Porosity of compound Dacron grafts made with 25% albumin was less than 1 ml/min/cm2 regardless of the fabric base, whereas porosity of grafts made with serum or 5% albumin was proportional to the porosity of the base fabric. Porosity of the compound grafts remained stable for more than 48 hours and to pressure greater than 450 mm Hg, if the grafts were kept moist. Tubes of Marlex mesh coated with heat-denatured albumin, implanted as infrarenal aortic replacements in dogs, showed complete albumin absorption by 3 weeks. However, perigraft tissue reaction and graft incorporation were minimal and extensive false aneurysm formation resulted. Knitted filamentous Dacron 6 mm tubes coated with heat-denatured albumin were implanted as iliofemoral bypass grafts in 12 dogs, with blood-preclotted knitted filamentous Dacron grafts implanted as contralateral control grafts. Comparison of the albumin-coated grafts with the blood-preclotted control grafts showed no differences in healing or patency at 4 to 6 months. Heat-denatured 25% albumin forms a strong and hemostatic coating regardless of fabric base. Albumin-Dacron compound grafts are easily and rapidly made in the operating room, handle well, and are suitable for large and medium-sized arterial replacements without changes in healing or patency. Because of slow tissue incorporation, however, albumin-coated knitted Dacron grafts should be avoided in patients who require long-term anticoagulation therapy.
D000418:Albumins; D000818:Animals; D001769:Blood; D001807:Blood Vessel Prosthesis / Q000009:adverse effects; D004285:Dogs; D005260:Female; D006083:Graft Occlusion, Vascular; D006470:Hemorrhage / Q000209:etiology; D006358:Hot Temperature; D008297:Male; D010949:Plasma; D011093:Polyethylene Terephthalates; D011095:Polyethylenes; D011126:Polypropylenes; D011183:Postoperative Complications / Q000209:etiology; D011489:Protein Denaturation
D000418:Albumins; C016674:Plastipore; D011093:Polyethylene Terephthalates; D011095:Polyethylenes; D011126:Polypropylenes