Action of citrinin on bacterial chromosomal and plasmid DNA in vivo and in vitro.
Citrinin, a mycotoxin of Penicillium citrinum and other species of the genera Penicillium and Aspergillus, caused the following effects at different concentrations in Escherichia coli. In vivo at 100 micrograms/ml single-strand breaks were caused in the chromosomal DNA. In the presence of 100 micrograms/ml, UV (254 nm)-induced DNA damage was repaired in the bacterial cells without need for a complete growth medium. At 300 micrograms/ml lambda ts prophage was induced in a lysogenic E. coli strain. In an E. coli strain carrying a F' lac plasmid, 4.7% of the cells displayed the Lac- phenotype after treatment with 200 micrograms of citrinin per ml, suggesting elimination of the F' factor. In vitro, DNA repair synthesis was observed at 5 micrograms of citrinin per ml in permeabilized cells, and replicative DNA synthesis was inhibited at 200 micrograms/ml. In these systems synthesis of stable RNAs was slightly diminished at 300 micrograms/ml, and protein synthesis was not affected at concentrations up to 450 micrograms/ml. Lambda and ColE1 plasmid DNA were cleaved in vitro when small amounts of copper ions were present. This DNA-attacking activity was prevented by NADPH, catalase, and superoxide dismutase and by higher concentrations of hydroxyl radical scavengers, suggesting the involvement of free radicals in the mechanism of action of citrinin on DNA.
D003086:Bacteriocin Plasmids / Q000187:drug effects; D010582:Bacteriophage lambda / Q000254:growth & development; D001578:Benzopyrans / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D002953:Citrinin / Q000494:pharmacology*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000187:drug effects*; D004279:DNA, Viral / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D005144:F Factor / Q000187:drug effects; D010957:Plasmids / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000494:pharmacology; D014775:Virus Activation / Q000187:drug effects
D001578:Benzopyrans; D004269:DNA, Bacterial; D004279:DNA, Viral; D002953:Citrinin; D002374:Catalase; D013482:Superoxide Dismutase; D004121:Dimethyl Sulfoxide