Cell uptake, distribution and response to aluminium chloro sulphonated phthalocyanine, a potential anti-tumour photosensitizer.
The uptake, retention and effects of aluminium chloro sulphonated phthalocyanine (AlSPc) were measured in two cell lines, UV-2237 a murine fibrosarcoma and the non-tumorigenic NIH/3T3 fibroblast line. The behaviour of cells treated with AlSPc was compared with that of those treated with haematoporphyrin derivative (HpD), a photosensitizer often used in photodynamic therapy (PDT) of cancer. AlSPc absorbs light strongly in the red region, is taken up by cells in a dose dependent fashion and is retained in vitro over a period of days (5 days after exposure greater than 40% remains cell-associated versus less than 25% of HpD). Additionally AlSPc was less cytotoxic to cells, maintained in darkness or exposed to room light, compared to HpD (100% viability versus 0% viability 3 days after 60 min exposure to room light). However red light (approximately 600-700 nm) caused greater toxicity in AlSPc-treated cells (100%) than in similarly exposed HpD-treated cells (less than 60%). No significant differences were detected between the responses of the fibrosarcoma and the fibroblast cell lines. These characteristics of AlSPc suggest that it may prove to be a useful photosensitizer for PDT of cancer and this possibility is discussed.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005354:Fibrosarcoma / Q000188:drug therapy*; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000627:therapeutic use; D007211:Indoles / Q000627:therapeutic use*; D054833:Isoindoles; D051379:Mice; D009942:Organometallic Compounds / Q000627:therapeutic use*; D010778:Photochemotherapy; D013053:Spectrophotometry; D013451:Sulfonic Acids / Q000627:therapeutic use
D006415:Hematoporphyrins; D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D013451:Sulfonic Acids; C044797:chloroaluminum phthalocyanine; D017324:Hematoporphyrin Derivative; C013647:phthalocyanine