title: Localization of visual pigment antigens to photoreceptor cells with different oil droplets in the chicken retina.
abstract: Frozen semithin sections and unembedded retinal pieces were investigated by immunocytochemistry using two antibodies produced against visual pigments in our laboratory. One was a polyclonal serum (AO) raised against bovine rhodopsin, while the other one was a monoclonal antibody (COS-1) produced against an epitope present in a cone visual pigment. AO stained, as expected, rod outer segments; in addition it also recognized a single cone characterized by a deep yellow oil droplet as well as another single cone with a yellowish green oil droplet. In contrast, COS-1 labelled both members of the double cones; the principal member having a yellowish-green oil droplet and the accessory member. COS-1 also stained a single cone type exhibiting a large red oil droplet.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens; D002645:Chickens; D006651:Histocytochemistry; D007120:Immunochemistry; D009821:Oils / Q000378:metabolism; D010786:Photoreceptor Cells / Q000276:immunology / Q000378:metabolism*; D012160:Retina / Q000276:immunology / Q000378:metabolism*; D012168:Retinal Pigments / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D009821:Oils; D012168:Retinal Pigments