Changes of phospholipase A2 inhibitory activity in the K+-sensitive actin gelation factor during the differentiation of myeloid leukemia cells.
The phospholipase A2 inhibitory activity of a 38 kDa K+-sensitive actin gelation factor in a murine leukemia cell line (M1) was examined. A specific antibody against 38 kDa protein was found to cross-react with 37 kDa protein (lipocortin) in rat peritoneal exudates. Although the native 38 kDa protein from M1 cells did not block phospholipase A2 activity, pretreatment with alkaline phosphatase produced a form that did inhibit this enzyme. However, a purified 38 kDa protein from differentiated M1 cells blocked phospholipase A2 activity without pretreatment with alkaline phosphatase. Phospholipase A2 inhibitory activity of the 38 kDa protein was not altered by addition of actin. These findings suggest that the phospholipase A2 inhibitory of our 38 kDa protein was induced during differentiation. We also proposed that our 38 kDa protein has the same epitope as lipocortin.
D000199:Actins / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D017302:Annexins; D002352:Carrier Proteins; D002454:Cell Differentiation; D005782:Gels; D006023:Glycoproteins; D007106:Immune Sera; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D008840:Microfilament Proteins / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D008970:Molecular Weight; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D011188:Potassium / Q000494:pharmacology*
D000199:Actins; D017302:Annexins; D002352:Carrier Proteins; C034037:F-actin-binding proteins; D005782:Gels; D006023:Glycoproteins; D007106:Immune Sera; D008840:Microfilament Proteins; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D000469:Alkaline Phosphatase; D011188:Potassium