The size of enzymes acetylating alpha-melanocyte-stimulating hormone and beta-endorphin.
Acetylation of alpha-melanocyte-stimulating hormone and beta-endorphin in a granule fraction from the rat pituitary neurointermediate lobe was examined by radiation inactivation. Target sizes obtained were 71 +/- 12 and 76 +/- 9 kDa, respectively. These data suggest that a single enzyme species may be responsible for acetylation of the two substrates. A similar analysis of alpha-melanocyte-stimulating hormone acetylation in the rat lens revealed a complex inactivation curve which could be explained by the presence of at least two different enzyme molecules, one of 86 and the other greater than 200 kDa.
D000818:Animals; D004723:Endorphins / Q000378:metabolism*; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology / Q000528:radiation effects; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism*; D008970:Molecular Weight; D010904:Pituitary Gland, Posterior / Q000201:enzymology / Q000528:radiation effects; D051381:Rats; D001615:beta-Endorphin
D004723:Endorphins; D001615:beta-Endorphin; D009074:Melanocyte-Stimulating Hormones