Transformation-related cellular protein p53: increased level in untransformed rat cells following treatment with the tumorpromoter, tetradecanoylphorbol-acetate.
12-O-Tetradecanoylphorbol-13-acetate (TPA, 100 ng ml-1), a tumor promoting phorbol ester, is able to induce enhanced levels of the transformation-associated cellular antigen p53 in normal rat 2 cells which had not been previously initiated by a carcinogen. p53 was estimated in ethanol-fixed treated cells on microtiter plates with ELISA using the monoclonal antibody Pab 1620 [EMBO J. 7, 1485, (1984)]. Induction of p53 was confirmed by immunoblotting. This effect of TPA is an additional phenotypic characteristic of tumor cells which can be induced by TPA in untransformed rodent cells.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004797:Enzyme-Linked Immunosorbent Assay; D007163:Immunosorbent Techniques; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D010704:Phorbols / Q000494:pharmacology*; D010750:Phosphoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D051381:Rats; D011916:Rats, Inbred F344; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53
D000911:Antibodies, Monoclonal; D009363:Neoplasm Proteins; D010704:Phorbols; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53; D013755:Tetradecanoylphorbol Acetate