Magnification of rRNA gene number in a Neurospora crassa strain with a partial deletion of the nucleolus organizer.
Some progeny from crosses between the Neurospora crassa translocation strain T(IL----VL)OY321 and normal sequence N. crassa strains are duplication strains with a partial deletion of the nucleolus organizer. Despite the deletion, these progeny are viable and produce a functional nucleolus. Quantification of rRNA gene number in these deletion progeny demonstrated a significant loss of rRNA genes, down to 60% of the parental wild-type level. Initially, all of these reduced nucleolus organizer (RNO) strains demonstrated a reduction in the rate of mycelial elongation in growth tubes. After several vegetative growth cycles some progeny reverted to the normal growth phenotype, and also showed an increase in the number of rRNA genes to approximately that of the wild type.
D003433:Crosses, Genetic; D005800:Genes, Fungal; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development; D009697:Nucleolus Organizer Region; D012335:RNA, Ribosomal / Q000235:genetics*; D014178:Translocation, Genetic
D012335:RNA, Ribosomal