Localization of visual pigment antigens to photoreceptor cells with different oil droplets in the chicken retina.
Frozen semithin sections and unembedded retinal pieces were investigated by immunocytochemistry using two antibodies produced against visual pigments in our laboratory. One was a polyclonal serum (AO) raised against bovine rhodopsin, while the other one was a monoclonal antibody (COS-1) produced against an epitope present in a cone visual pigment. AO stained, as expected, rod outer segments; in addition it also recognized a single cone characterized by a deep yellow oil droplet as well as another single cone with a yellowish green oil droplet. In contrast, COS-1 labelled both members of the double cones; the principal member having a yellowish-green oil droplet and the accessory member. COS-1 also stained a single cone type exhibiting a large red oil droplet.
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens; D002645:Chickens; D006651:Histocytochemistry; D007120:Immunochemistry; D009821:Oils / Q000378:metabolism; D010786:Photoreceptor Cells / Q000276:immunology / Q000378:metabolism*; D012160:Retina / Q000276:immunology / Q000378:metabolism*; D012168:Retinal Pigments / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000941:Antigens; D009821:Oils; D012168:Retinal Pigments