46 kd mannose 6-phosphate receptor: cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor.
We have isolated cDNA clones encoding the entire sequence of the bovine 46 kd cation-dependent mannose 6-phosphate (CD Man-6-P) receptor. Translation of CD Man-6-P receptor mRNA in Xenopus laevis oocytes results in a protein that binds specifically to phosphomannan-Sepharose, thus demonstrating that our cDNA clones encode a functional receptor. The deduced 279 amino acid sequence reveals a single polypeptide chain that contains a putative signal sequence and a transmembrane domain. Trypsin digestion of microsomal membranes containing the receptor and the location of the five potential N-linked glycosylation sites indicate that the receptor is a transmembrane protein with an extracytoplasmic amino terminus. This extracytoplasmic domain is homologous to the approximately 145 amino acid long repeating domains present in the 215 kd cation-independent Man-6-P receptor.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D005796:Genes; D008360:Mannosephosphates / Q000378:metabolism; D008970:Molecular Weight; D009865:Oocytes / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D014176:Protein Biosynthesis; D017527:Receptor, IGF Type 2; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014357:Trypsin; D014982:Xenopus laevis
D002352:Carrier Proteins; D008360:Mannosephosphates; D010446:Peptide Fragments; D017527:Receptor, IGF Type 2; D014357:Trypsin