title: Alpha-h-ANP is the only form of circulating ANP in humans.
abstract: Alpha-human atrial natriuretic peptide (alpha-h-ANP) was purified to homogeneity from human plasma of healthy adults in a three-step procedure including immunoaffinity chromatography on immobilized monoclonal anti-alpha-h-ANP antibody (moab). A single peak of immunoreactivity was obtained after final reversed-phase HPLC and the amino acid sequence of the isolated material was identical to that of synthetic alpha-h-ANP. No further atrial peptides could be detected although the moab reacts with all biologically active peptides. It is therefore concluded that alpha-h-ANP is the only form of ANP circulating in human plasma.
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D011498:Protein Precursors / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D011498:Protein Precursors; D009320:Atrial Natriuretic Factor