[Precise mapping of the gpp gene involved in guanosine tetraphosphate synthesis and ilvC-gpp deletion in the region of the Escherichia coli chromosome].
Using the set of transducing lambda phages the gpp gene, responsible for pppGpp to ppGpp conversion, was localized between rep and trxA genes on 85 min of the Escherichia coli genetic map. Taking advantage of the Tn10 transposon inserted into the adjacent ilvY locus, we deleted the region of E. coli chromosome covering ilvC, rep and gpp genes. The metabolism of (p)ppGpp in the deletion-containing cells confirms that the product of the gpp gene, guanosine pentaphosphatase, is not the only enzyme, responsible for pppGpp degradation and ppGpp synthesis.
D010582:Bacteriophage lambda / Q000235:genetics; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D006150:Guanine Nucleotides / Q000096:biosynthesis*; D006159:Guanosine Tetraphosphate / Q000096:biosynthesis* / Q000235:genetics; D014161:Transduction, Genetic
D006150:Guanine Nucleotides; D006159:Guanosine Tetraphosphate
Tochnoe kartirovanie gena gpp, uchastvuiushchego v sinteze guanozintetrafosfata, i poluchenie deletsii ilvC-gpp v oblasti khromosomy Escherichia coli.