A simple method for simultaneous detection of Fc receptors and differentiation antigens on normal and neoplastic murine hematopoietic cells.
Cell surface receptors for immunoglobulin Fc regions can be detected by light microscopy of cytocentrifuge preparations of normal or malignant murine hematopoietic cells. The cells are first incubated in a culture supernate containing a monoclonal anti-hapten antibody, then with heat-killed, haptenated bacteria. Using 2 morphologically distinct strains of bacteria, we can detect both Fc receptors and cell surface differentiation antigens.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D002460:Cell Line; D004926:Escherichia coli / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000032:analysis* / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis; D007942:Leukemia, Experimental / Q000276:immunology*; D008264:Macrophages / Q000032:analysis / Q000276:immunology; D008407:Mast Cells / Q000032:analysis / Q000276:immunology; D051379:Mice; D014411:Neoplastic Stem Cells / Q000032:analysis / Q000276:immunology; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000032:analysis; D013211:Staphylococcus aureus / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006241:Haptens; D011961:Receptors, Fc; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D007073:Immunoglobulin E