title: High-field n.m.r. studies of keratan sulphates. 1H and 13C assignments of keratan sulphate from shark cartilage.
abstract: Keratan sulphate was extracted from a shark/whale cartilage preparation and examined by 400 MHz 1H- and 100 MHz 13C-n.m.r. spectroscopy. Assignment of the majority of the resonances was facilitated by two-dimensional 13C-1H correlation by using a modified COLOC procedure and a COSY-45 experiment. The spectra are consistent with an N-acetyl-lactosamine repeating unit that is predominantly sulphated at C-6 of both galactose and N-acetylglucosamine. Gel chromatography of a keratanase digest of the shark keratan sulphate confirmed the high degree of galactose sulphation.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D005690:Galactose / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans; D007632:Keratan Sulfate; D009682:Magnetic Resonance Spectroscopy; D012754:Sharks / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D007632:Keratan Sulfate; D005944:Glucosamine; D005690:Galactose