title: A simple method for simultaneous detection of Fc receptors and differentiation antigens on normal and neoplastic murine hematopoietic cells.
abstract: Cell surface receptors for immunoglobulin Fc regions can be detected by light microscopy of cytocentrifuge preparations of normal or malignant murine hematopoietic cells. The cells are first incubated in a culture supernate containing a monoclonal anti-hapten antibody, then with heat-killed, haptenated bacteria. Using 2 morphologically distinct strains of bacteria, we can detect both Fc receptors and cell surface differentiation antigens.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D002460:Cell Line; D004926:Escherichia coli / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000032:analysis* / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis; D007942:Leukemia, Experimental / Q000276:immunology*; D008264:Macrophages / Q000032:analysis / Q000276:immunology; D008407:Mast Cells / Q000032:analysis / Q000276:immunology; D051379:Mice; D014411:Neoplastic Stem Cells / Q000032:analysis / Q000276:immunology; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000032:analysis; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006241:Haptens; D011961:Receptors, Fc; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D007073:Immunoglobulin E