Migratory capacity of large granular lymphocytes from T-gamma-lymphoproliferative disorders.
We have investigated 10 patients with T gamma-lymphoproliferative disorders (T gamma LPD) for cell migratory capacity. Large granular lymphocytes (LGLs) expanding in these subjects expressed natural killer cell markers (HNKI, B73.1, AB8.28, N901, OKM1) to a variable extent. The patients' LGLs mediated antibody-dependent cellular cytotoxicity, while appreciable natural killer activity was only measurable in 3 patients. The migratory capacity of T gamma LPD was examined by using nitrocellulose filters. The patients' LGLs migrated into filters and showed responsiveness (in 9 of 10 patients) to activated serum, used as chemoattractant, as normal LGLs do. No clearcut correlation emerged between in vitro migratory capacity and disease aggressiveness or involvement of abdominal organs. These results confirm in T gamma LPD the migratory potential of LGLs and suggest the possibility that acquisition of enhanced locomotor capacity is not a crucial determinant of disease aggressiveness in T gamma LPD.
D000328:Adult; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D002465:Cell Movement; D003101:Collodion / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006529:Hepatomegaly / Q000097:blood; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000502:physiology*; D008232:Lymphoproliferative Disorders / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011961:Receptors, Fc / Q000276:immunology; D017452:Receptors, IgG
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D011961:Receptors, Fc; D017452:Receptors, IgG; D003101:Collodion