[A new procedure for the direct demonstration of microbial aminoacylase on agar plates with o-phthalaldehyde].
Not less than 1 nmol of methionine and phenylalanine were detected as fluorescent spots on agar plates with o-phthalaldehyde reagent under UV light. Microorganisms were grown on thin-agar-layer coated filter papers placed on nutrient agar plates, and then transferred onto new plates lacking nutrients by moving the papers. After the background amino compounds were removed by the diffusion to the bottom plates, the paper cultures were moved and incubated on assay plates containing N-acetylmethionine or N-acetylphenylalanine. The amino acids formed around the colonies were visualized by the o-phthaladehyde procedure to indicate aminoacylase activity of microorganisms. The substrate-and stereospecificity of the enzyme was shown for some strains on the agar plates by this procedure.
D000362:Agar; D000581:Amidohydrolases / Q000032:analysis*; D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D008722:Methods; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development; D009764:o-Phthalaldehyde
D009764:o-Phthalaldehyde; D000362:Agar; D000581:Amidohydrolases; C019672:aminoacylase I
Ein neues Verfahren zum direkten Nachweis mikrobieller Aminoacylaseaktivität auf Agarplatten mit o-Phthalaldehyd.