Transport and anatomic distribution of a high molecular weight fraction of bovine pituitary intraglandular colloid of intermediate lobe origin.
Bovine pituitary intraglandular colloid (IGC) of intermediate lobe (IL) origin, was separated into seven primary fractions (F1-F7) on a 2.6 X 70 cm column of dextran gel G-100 superfine (Pharmacia). The molecular weight of F1-F3 was much greater than 1000 000; F4, 63 000; F5, 34 000 and F6-F7 less than 4000. The first fraction (F1) was used in this study and was labelled with (125I)Na via a modification of the chloramine-T method. Male Wistar rats were divided into 5 experimental and 5 control groups. The animals were fed Purina Lab Chow and Lugol's iodine was mixed with the drinking water to minimize any uptake by the thyroid gland of 125I liberated by metabolism. Under anesthesia, the animals in the experimental group were administered via the right internal jugular vein, 0.15 ml of (125I)F1 and those in the control were administered with 0.15 ml of free (125I)NA. Animals were killed with sodium pentobarbital at 2, 4, 6, 12 and 24 h periods. There are 5 replications for each of the 5 treatments (time periods) in every experiment including the control. Twenty-one tissues were retrieved and the kidney, spleen and thyroid were the only tissues that had retained the (125I)F1 at the 24 h recording period. Autoradiographic labelling was detected in hematoxylin-eosin stained sections of these tissues. Although it is difficult to make an exact determination as to whether the labelled fraction recovered is the same as the one injected, the kidney, spleen and thyroid continued to be significant tissues after several repeated experiments. The autoradiographic picture was the same each time the experiment was repeated. These results tend to indicate that IGC of IL origin is involved in the transport of IL materials.
D000818:Animals; D001345:Autoradiography; D001692:Biological Transport; D002417:Cattle; D003102:Colloids / Q000032:analysis / Q000378:metabolism*; D007668:Kidney / Q000032:analysis / Q000378:metabolism; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000032:analysis / Q000378:metabolism; D013961:Thyroid Gland / Q000032:analysis / Q000378:metabolism; D014018:Tissue Distribution
D003102:Colloids