title: Adenovirus serotype determines association and localization of the large E1B tumor antigen with cellular tumor antigen p53 in transformed cells.
abstract: The distribution and stability of the cellular tumor antigen p53 were studied in baby rat kidney cells transformed by region E1 sequences of nononcogenic adenovirus (Ad) type 5 (Ad5) or oncogenic type 12 (Ad12). In transformed cells expressing the large E1B T antigen of Ad5, p53 was associated with this T antigen. The complexed proteins were concentrated in a cytoplasmic body, which has been shown to consist of a cluster of 8-nm filaments (A. Zantema et al., Virology 142:44-58, 1985). In transformed cells expressing the E1B region of Ad12, however, no association between the viral large T antigen and p53 was detectable. In the latter case, both proteins were found almost exclusively in the nucleus. The stability of p53 in both Ad5- and Ad12-transformed cells was increased relative to that in primary cells or cells immortalized by the E1A region only. Thus, the increased stability of p53 in Ad-transformed cells is not caused by association with a viral T antigen, but it correlates with expression of E1B and with morphological transformation.
mesh_terms: D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D005455:Fluorescent Antibody Technique; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000032:analysis*; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis*; D051381:Rats; D012703:Serotyping; D014162:Transfection; D016159:Tumor Suppressor Protein p53
chemical_list: D017456:Adenovirus Early Proteins; D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor; D009363:Neoplasm Proteins; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53