[Analysis of the gamma interferon receptor in mice].
Natural murine interferon-gamma (IFN-gamma) produced by the T lymphoma, L12R4, stimulated with phorbol myristic acetate was purified by anti-murine IFN-gamma immunoadsorbent and labeled with 125I to study its binding to receptors of mouse cells. By adding increasing concentrations of unlabeled natural murine IFN-gamma in competition binding assays we determined a KD of 8.2 X 10(-10) M for L1210 cells and a number of receptors of about 3000. Moreover protease, but not endoglycosidase treatment of target cells prevented the subsequent binding of the ligand. These studies demonstrate that natural murine IFN-gamma binds in a specific manner and with high affinity to receptors on murine cell lines.
D000818:Animals; D002460:Cell Line; D007163:Immunosorbent Techniques; D007371:Interferon-gamma / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism; D051379:Mice; D011971:Receptors, Immunologic / Q000032:analysis*; D017471:Receptors, Interferon; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate
Analisi del recettore per l'interferon gamma murino.