An evaluation of the existence of soluble suppressor factors in cloned antigen-specific T suppressor lymphocytes.
The suppressive activity of two bovine serum albumin-specific class II-restricted T suppressor cell clones (BVI/5 and 83/2-D11) was compared to that of a feeder cell-independent, IL 2-dependent subline (HF1.IL-2) of an originally antigen-dependent class II-restricted Ts cell clone (HF1). No soluble suppressor factors can be found in BVI/5 and 83/2-D11 Ts cell extracts or culture supernatants under conditions where an unspecific factor can be derived from HF1.IL-2 cells. This factor, when isolated from cell extracts in the presence of n-octyl-beta-D-glycopyranoside, has a molecular weight of 70-80 kD. In absence of this non-ionic detergent, it has a high affinity to membrane fragments and is associated with a molecular weight of 300 kD or more. The data are discussed in connection with recent findings of direct T suppressor to T helper interaction by cell-cell contact.
D000818:Animals; D000941:Antigens / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008970:Molecular Weight; D012710:Serum Albumin, Bovine / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000302:isolation & purification*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000941:Antigens; D000949:Histocompatibility Antigens Class II; D013491:Suppressor Factors, Immunologic; D012710:Serum Albumin, Bovine