title: Damages induced in lambda phage DNA by enzyme-generated triplet acetone.
abstract: Exposure of lambda phage to triplet acetone, generated via the oxidation of isobutanal by peroxidase, leads to genome lesions. The majority of these lesions are detected as DNA single-strand breaks only under alkaline conditions, and so true breaks do not occur. Also, no sites sensitive to UV-endonuclease from Micrococcus luteus were found in DNA from treated phage. The participation of triplet acetone in the generation of such DNA damage is discussed.
mesh_terms: D000433:1-Propanol / Q000494:pharmacology; D000096:Acetone / Q000031:analogs & derivatives*; D000447:Aldehydes / Q000378:metabolism; D010582:Bacteriophage lambda / Q000187:drug effects* / Q000235:genetics; D002374:Catalase / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D003500:Cyclization; D004279:DNA, Viral / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004706:Endodeoxyribonucleases; D006735:Horseradish Peroxidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009097:Multienzyme Complexes / Q000378:metabolism; D009699:N-Glycosyl Hydrolases; D010084:Oxidation-Reduction; D020005:Propanols; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D000447:Aldehydes; D004279:DNA, Viral; D009097:Multienzyme Complexes; D020005:Propanols; C037881:triplet acetone; D000096:Acetone; C032219:UV endonuclease; D000433:1-Propanol; C017439:isobutyraldehyde; D006735:Horseradish Peroxidase; D002374:Catalase; D013482:Superoxide Dismutase; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; D009699:N-Glycosyl Hydrolases