[Protective effect of alpha-tocopherol on the Ca2+-transport system of the sarcoplasmic reticulum membranes in hypercholesterolemia].
The effect of antioxidant--alpha-tocopherol--on Ca2+-transporting system in sarcoplasmic reticulum (SR) of the rabbit skeletal muscles was studied in hypercholesterolemia (HC). alpha-tocopherol administration to animals with HC produced a break on the curve of temperature dependence of Ca-ATPase activity at about 20 degrees C, that disappeared in HC, increased the rate of "rapid" SH-group binding by thiol reagents, and normalized the level of unsaturated fatty acids in SR membranes without altering phospholipid content. It is suggested that the damage of Ca-ATPase in HC is mainly due to activation of lipid peroxidation.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D006937:Hypercholesterolemia / Q000188:drug therapy* / Q000378:metabolism; D007425:Intracellular Membranes / Q000187:drug effects*; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects*; D013696:Temperature; D014810:Vitamin E / Q000627:therapeutic use*
D008054:Lipid Peroxides; D014810:Vitamin E; D000252:Calcium-Transporting ATPases; D002118:Calcium
Zashchitnoe vliianie al'fa-tokoferola na Ca2+-transportiruiushchuiu sistemu membran sarkoplazmaticheskogo retikuluma pri giperkholesterinemii.