title: Transcription of Neurospora crassa 5 S rRNA genes requires a TATA box and three internal elements.
abstract: The sequences required for transcription of Neurospora crassa 5S rRNA genes have been defined using a comprehensive set of deletion and substitution mutations introduced into two cloned genes. An upstream TATA box (consensus TCATAGA) located at -29 to -24, and three internal regions (D, A and C) localized to +19 to +30, +44 to +57 and +73 to +103, respectively, are absolutely required for transcription in vitro. The TATA box fixes the start point of transcription. The A and C regions correspond to the Xenopus 5S gene internal control region but the D region has no Xenopus homologue. The spacing of the three internal elements is very strict but the position of the TATA box can be varied by up to 16 base-pairs.
mesh_terms: D001483:Base Sequence; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S