title: Enzyme-amplified immunoassays: a new ultrasensitive assay of thyrotropin evaluated.
abstract: An enzyme-amplified immunoassay for thyrotropin has been evaluated. The lower limit of detection of the assay (mean + 3 SD of the zero standard) was 0.037 milli-int.unit/L and the precision was good, giving a working range of 0.13-24.0 milli-int.units/L. The accuracy of the assay was good, as judged from analytical-recovery experiments, analysis of external quality-assessment samples and comparison with in-house assays. No significant interferences or cross reactivities were identified. Assay of a 25-microL serum sample on the microtiter plate takes 3 h. Values for healthy subjects ranged from 0.4 to 4.0 milli-int.units/L. For 32 thyrotoxic patients thyrotropin concentrations were clearly suppressed and completely distinct from normal values. Patients taking triiodothyronine also showed lower concentrations. The enzyme-amplified immunoassay may thus be a suitable "front line" test for assessing thyroid function.
mesh_terms: D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases; D000911:Antibodies, Monoclonal; D002384:Catalysis; D008058:Dihydrolipoamide Dehydrogenase; D005069:Evaluation Studies as Topic; D006801:Humans; D006980:Hyperthyroidism / Q000097:blood; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D009243:NAD; D011863:Radioimmunoassay; D013972:Thyrotropin / Q000097:blood*
chemical_list: D000911:Antibodies, Monoclonal; D009243:NAD; D013972:Thyrotropin; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase