Characterization of murine cytolytic-helper hybrid T cell clones.
L3T4, Lyt-2 and the T-cell receptor for antigen are cell-surface molecules involved in antigen specific T cell activation. We have constructed functional murine cytolytic-helper T-cell hybrid clones to study the link between expression of cell-surface molecules and specific cell function. Three of the clones express two antigen receptors and both Lyt-2 and L3T4, normally expressed on mutually exclusive subsets of mature T lymphocytes. The pattern of lymphokines produced by the hybrid cells in response to antigen was not controlled by the specific antigen receptor; both T-cell growth factor, produced only by the helper T-cell partner, and gamma-interferon, produced only by the cytolytic T-cell partner, were secreted when either antigen receptor was stimulated. However, cytolytic activity appeared to be restricted to the recognition of antigen by the T-cell receptor of the cytolytic partner. Thus cytolysis appears to be rightly linked to the antigen receptor of the cytolytic parent but lymphokine release is not tightly linked.
D000818:Animals; D000911:Antibodies, Monoclonal; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006822:Hybrid Cells; D007518:Isoantibodies / Q000032:analysis; D008222:Lymphokines / Q000032:analysis; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology*
D000911:Antibodies, Monoclonal; D007518:Isoantibodies; D008222:Lymphokines; C029805:Lyt antibodies