[Cytofluorimetric study of ploidy in the preleukemic thymus in C57BL/Ka mice under fractionated irradiation].
The ploidy of the thymus was studied in C57B1/Ka mice irradiated with 4 weekly X-Ray doses of 1.75 Gy. The determination of nuclear DNA content was performed by flow cytometry of intact thymocytes labeled with propidium iodide in presence of a mixture of chicken and rainbow trout red blood cells as internal reference standards. The method has been tested by detecting the sex difference in DNA content of G0/G1 of normal thymic mouse cells. The mean value was 2.9% higher in female mice. The thymus of almost 60% of irradiated male mice present a slight hypoploidy of 2.6% one month after the last irradiation.
D000818:Animals; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011003:Ploidies / Q000528:radiation effects*; D011289:Preleukemia / Q000235:genetics*; D013950:Thymus Gland / Q000473:pathology / Q000528:radiation effects* / Q000648:ultrastructure; D014916:Whole-Body Irradiation
Etude cytofluorimétrique de la ploïdie dans le thymus préleucémique chez la Souris C57B1/Ka soumise à une irradiation fractionnée.