A human-human hybridoma secreting anti-HLA class II antibody.
In this report, we describe the production and characterization of the first human-human hybridoma secreting antibody to HLA Class II determinants. The hybridoma (GMEC101), which has been stable in tissue culture for greater than 20 mo, secretes 10 to 50 micrograms/ml of IgM-kappa antibody. This antibody binds to a wide range of human cell lines, but not to the HLA-A,B,C, and DR-negative K562 cell line. Functionally, GMEC101 strongly inhibits a unidirectional mixed lymphocyte reaction (MLR) at the level of the stimulator cell. Neither the cellular ELISA binding nor the MLR inhibition is lost after a triple platelet absorption (which removes Class I but not Class II activity). Because the binding and MLR blocking show no correlation with the known DR or DQ specificities, we suggest that GMEC101 may be detecting a novel HLA Class II determinant.
D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology* / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000276:immunology* / Q000378:metabolism; D007518:Isoantibodies / Q000276:immunology* / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D008297:Male
D000949:Histocompatibility Antigens Class II; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D007518:Isoantibodies