The influenza C virus NS gene: evidence for a spliced mRNA and a second NS gene product (NS2 protein).
It has previously been shown that the shortest RNA (RNA 7) of influenza C viruses codes for a nonstructural (NSI) protein (Nakada et al. (1985) J. Virol. 56, 221-226). Experiments reported here indicate that RNA 7 also directs the synthesis of a second nonstructural protein via a spliced mRNA. The amino terminal 62 codons of this NS2 protein appear to be shared with the NS1 protein and the carboxyl terminal 59 amino acids are unique (derived from a +1 open reading frame in the mRNA). Although the size of the C virus NS2 protein is comparable to that of the A and B virus NS2 proteins, the overall arrangement of the C virus NS gene is quite different from that of the A and B virus NS genes. The second (+1) open reading frame of the C virus NS gene is completely overlapped by that of the NS1 protein, whereas the second (+1) open reading frame of the A and B virus NS genes extends to the 3' end of the RNA and only partially (or in some strains not at all) overlaps the NS1 open reading frame.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D009982:Gammainfluenzavirus / Q000235:genetics*; D009975:Orthomyxoviridae / Q000235:genetics*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D011817:Rabbits; D014158:Transcription, Genetic; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*
D012333:RNA, Messenger; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins