title: Mutational analysis of the lambda int gene: DNA sequence of dominant mutations.
abstract: We have combined techniques of genetic and physical mapping with rapid DNA sequence analysis to identify the nucleotide change in lambda int mutations. These mutations define two dominant phenotypic classes: (i) recombination that is partially independent of accessory factors, and (ii) inhibition of wild-type Int by missense or nonsense proteins, i.e., negative complementation.
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D008242:Lysogeny; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype
chemical_list: D004279:DNA, Viral