Comparison of serum fructosamine with glycosylated serum protein (determined by affinity chromatography) for the assessment of diabetic control.
Glycosylated total protein (GTP) and glycosylated albumin (GALb) were measured in serum using aminophenylboronic acid affinity chromatography and the results were compared with those found using the fructosamine assay. The percentage GTP and GALb found by affinity chromatography correlated well with fructosamine values in the sera of a group of non-diabetic and diabetic patients (fructosamine vs GTP, r = 0.91, p less than 0.001; fructosamine vs GALb, r = 0.91, p less than 0.001). Results of each method gave similar correlations when compared with the degree of diabetic control assessed by glycosylated haemoglobin (GHb) and fasting plasma glucose (FPG) (fructosamine vs FPG, r = 0.74, p less than 0.001; GTP vs FPG, r = 0.75, p less than 0.001; GALb vs FPG, r = 0.79, p less than 0.001; fructosamine vs GHb, r = 0.79, p less than 0.001; GTP vs GHb, r = 0.81, p less than 0.001; GALb vs GHb, r = 0.84, p less than 0.001). Both methods could equally discriminate between groups of non-diabetics and diabetic patients (p less than 0.001) and showed similar temporal changes after starting insulin therapy.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001786:Blood Glucose / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis*; D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D019270:Fructosamine; D006023:Glycoproteins; D006595:Hexosamines / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000379:methods*; D000093422:Glycated Serum Proteins
D001786:Blood Glucose; D001798:Blood Proteins; D006023:Glycoproteins; D006595:Hexosamines; D019270:Fructosamine; D000093422:Glycated Serum Proteins