Selenium-induced enhancement of hematoporphyrin derivative phototoxicity in murine bladder tumor cells.
The phototoxicity of hematoporphyrin derivative (Hpd) to murine bladder tumor (MBT-2) cells was studied in vitro. It was observed that selenium in the form of sodium selenite enhanced Hpd-sensitized photodamage in MBT-2 cells under conditions where selenite alone was non-toxic. Sodium selenite enhanced the fluorescence emission of Hpd and augmented the Hpd-sensitized photooxidation of tryptophan. The data suggest that sodium selenite is able to disaggregate Hpd, thereby enhancing Hpd-sensitized phototoxicity.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004347:Drug Interactions; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000494:pharmacology*; D008027:Light; D051379:Mice; D010777:Photochemistry; D020887:Selenious Acid; D012643:Selenium / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000528:radiation effects; D001749:Urinary Bladder Neoplasms
D006415:Hematoporphyrins; D017324:Hematoporphyrin Derivative; D014364:Tryptophan; D020887:Selenious Acid; D012643:Selenium