Human T4+ T-lymphocyte clones specific for the B fragment of tetanus toxin.
Two T4+ cloned T-lymphocyte lines specific for a papain digest product of tetanus toxin are functionally characterized. The two clones were obtained from peripheral blood mononuclear cells activated in vitro by tetanus toxoid, expanded with IL-2, and cloned in soft agar. Both clones could be induced to undergo blastogenesis with tetanus toxoid, tetanus toxin, and the B fragment but not the C fragment of tetanus toxin. In addition, both clones caused cytolysis of plastic adherent cell targets cocultured for 18 hr with either tetanus or the B fragment. Antigen specific proliferation and cytolytic activity were MHC-class I restricted.
D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013744:Tetanus Toxin / Q000276:immunology* / Q000494:pharmacology; D013745:Tetanus Toxoid / Q000494:pharmacology
D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D010446:Peptide Fragments; D013744:Tetanus Toxin; D013745:Tetanus Toxoid; C040635:tetanus toxin fragment C