Binding of chicken, bovine, and rabbit immunoglobulins by avian, bovine, and human strains of Staphylococcus aureus.
Sixty-six strains of Staphylococcus aureus of avian, bovine, and human origin were tested for their ability to bind chicken, bovine, and rabbit immunoglobulin G (IgG). A microtitration plate hemagglutination assay and a direct-tube enzyme immunoassay were used to determine qualitative differences. Twice as many chicken and bovine S. aureus isolates than human strains reacted positively to chicken IgG. Mean binding values of chicken IgG were also twice as high for chicken and bovine S. aureus isolates when compared with human-derived strains. Isolates of bovine and human origin displayed a high affinity for bovine IgG and rabbit IgG, whereas few isolates from chickens bound substantial quantities of the nonavian IgG species. These results demonstrate that preparations of staphylococcal protein A with affinities for immunoglobulins from poultry and other animals can be obtained by screening large numbers of isolates, especially those obtained from an animal species, such as chickens, that is the same as the source of the immunoglobulin one wishes to bind.
D000818:Animals; D002417:Cattle; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology*; D011200:Poultry; D011201:Poultry Diseases / Q000276:immunology; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000276:immunology*; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000276:immunology / Q000662:veterinary; D013211:Staphylococcus aureus / Q000276:immunology* / Q000302:isolation & purification; D014422:Turkeys
D007074:Immunoglobulin G; D017452:Receptors, IgG; D011971:Receptors, Immunologic