title: Hepatitis B surface antigen polypeptide micelles from antigen expressed in Saccharomyces cerevisiae.
abstract: Hepatitis B micelles containing the p25 component of hepatitis B surface antigen (HBsAg) have been produced by Triton X-100 solubilization followed by ultracentrifugation in linear sucrose gradients. The product was found to resemble micelle forms prepared from plasma-derived HBsAg with the surface being composed of discrete globular and stranded sub-units. The degree of immunochemical relatedness of the micellular preparation was compared to the native 22-nm HBsAg particle present in either plasma or yeast cell extracts. The yeast micelle preparation competed for anti-HBs in a similar manner as intact HBsAg of plasma origin. Enhanced immunogenicity may be expected for micelles containing a recombinant HBsAg protein as has previously been shown for the plasma-derived antigen.
mesh_terms: D002499:Centrifugation, Density Gradient; D000090266:Chromobox Protein Homolog 5; D003102:Colloids; D004591:Electrophoresis, Polyacrylamide Gel; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D008823:Micelles; D008854:Microscopy, Electron; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology; D011863:Radioimmunoassay; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014761:Viral Hepatitis Vaccines
chemical_list: C000715594:CBX5 protein, human; D003102:Colloids; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D008823:Micelles; D010455:Peptides; D014761:Viral Hepatitis Vaccines; D000090266:Chromobox Protein Homolog 5