title: Calcium binding protein from porcine intestine binds to phosphatidylserine vesicles in the presence of calcium.
abstract: Protein II, a 32K cytoskeleton-associated protein isolated from porcine intestinal epithelium, binds to vesicles composed of phosphatidylserine in the presence, but not the absence, of 10 microM Ca2+. Binding was saturable and was specifically inhibited by chelation of free Ca2+ with EGTA. Binding was also inhibited by trifluophenothiazine. Vesicles composed of dimyristoylphosphatidylcholine did not bind protein II, suggesting that interaction with phosphatidylserine was selective. These properties are consistent with a possible role for protein II in Ca-regulated cytoskeleton-cell membrane events.
mesh_terms: D000818:Animals; D017314:Annexin A4; D002118:Calcium / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D004848:Epithelium / Q000032:analysis; D007422:Intestines / Q000032:analysis*; D007457:Iodine Radioisotopes; D008081:Liposomes / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism*; D013552:Swine
chemical_list: D017314:Annexin A4; D002135:Calcium-Binding Proteins; D007457:Iodine Radioisotopes; D008081:Liposomes; D010718:Phosphatidylserines; D004533:Egtazic Acid; D002118:Calcium