[Transfer of prophage Mu into methylotrophic bacteria in the plasmid RP4].
Bacteriophage Mu genome has been transferred into the cells of Pseudomonas methanolica and Methylobacterium sp. SKF240, that are naturally resistant to the bacteriophage, as a fragment of a hybrid plasmid RP4::Mu cts62. Temperature induction of the bacteriophage results in host cell lysis. Plasmid RP::Mu cis62 is maintained in methylotrophic cells presenting a cointegrative structure. The genetic and electrophoretic, analyses of the DNA isolated from transconjugant cells have confirmed the conclusion. Bacteriophage Mu propagation has been shown to be restricted in methylotrophic cells.
D010583:Bacteriophage mu / Q000235:genetics*; D005819:Genetic Markers; D008242:Lysogeny; D008772:Methylococcaceae / Q000235:genetics*; D010957:Plasmids; D014775:Virus Activation
D005819:Genetic Markers
Peredacha profaga Mu metilotrofnym bakteriiam v sostave plazmidy RP4.