Identification and isolation of vitamin K-dependent proteins by HPLC.
Six of the seven known vitamin K-dependent proteins found in plasma were chromatographed on a large-pore propylsilane column using aqueous trifluoroacetic acid/acetonitrile gradients. Prothrombin and Factor VII coeluted, the others were readily resolved. The technique has been used to monitor the purification of protein C and protein S using immobilized anti-protein S. Preliminary evidence is presented which is suggestive of the existence of additional vitamin K-dependent proteins in plasma.
D001798:Blood Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005164:Factor IX / Q000032:analysis; D005167:Factor VII / Q000032:analysis; D005170:Factor X / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D011486:Protein C / Q000032:analysis; D011499:Protein Processing, Post-Translational; D017293:Protein S; D011516:Prothrombin / Q000302:isolation & purification; D014812:Vitamin K / Q000502:physiology*
D001798:Blood Proteins; D006023:Glycoproteins; D011486:Protein C; D017293:Protein S; D014812:Vitamin K; D005167:Factor VII; D011516:Prothrombin; D005164:Factor IX; D005170:Factor X