Ultrastructure of multilayered cultures of human gingival epithelial cells: attachment to enamel surfaces in vitro.
Human gingival cells were collected using an enzymatic procedure and seeded on a feeder layer of irradiated mouse 3T3 fibroblasts. Epithelial cells generate stratified colonies ultimately forming an epithelium which was studied using electron microscopy. When this epithelium-like structure was dispased and transferred to enamel surfaces, the relationship between basal cells and enamel corresponded to half-desmosomes and a discontinuous extracellular matrix.
D000328:Adult; D002448:Cell Adhesion; D002478:Cells, Cultured; D003743:Dental Enamel / Q000166:cytology; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure*; D005881:Gingiva / Q000166:cytology / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron