Establishment, identification and characterization of a new sheep sinus tumor cell line.
A cell line designated SRT was established from a sheep sinus tumor. Following primary culture, the cells were serially passaged 40 times. SRT cells maintained an epithelioid fibroblast-like appearance and had a population doubling time of approximately 18 hr. Karyotype analysis of 14th passage cells showed the modal 2n chromosome number to be between 46 to 60, due to a large variation in acrocentric chromosome number. The electrophoretic mobilities of enzymes extracted from SRT cells were identical with those from normal sheep sinus cells. It propagated a number of ovine, bovine and canine viruses. Some virus-like particles (80-120 nm) were observed under the electron microscope. The tumor origin, good growth and wide range of virus susceptibility make SRT a highly suitable cell line for in vitro cancer research and for comparative virology studies.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D007621:Karyotyping; D008854:Microscopy, Electron; D010255:Paranasal Sinus Neoplasms / Q000473:pathology / Q000648:ultrastructure / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000473:pathology*; D014780:Viruses / Q000254:growth & development