Selective precipitation of phosphofructokinase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from rat erythrocyte hemolysates by polyethylene glycol.
Precipitation profiles of phosphofructokinase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase have been established in the range of 0-16% PEG at different pH values. Precipitation generally occurred between narrow limits of polyethylene glycol. The polymer concentration needed to reach any level of enzyme precipitation is dependent on pH. Particular conditions (% PEG and pH) for the selective enzyme enrichment have been determined.
D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000302:isolation & purification*; D011232:Chemical Precipitation; D004912:Erythrocytes / Q000201:enzymology*; D005948:Glucose Dehydrogenases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008297:Male; D010732:Phosphofructokinase-1 / Q000302:isolation & purification*; D010734:Phosphogluconate Dehydrogenase / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains
D011092:Polyethylene Glycols; D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D010734:Phosphogluconate Dehydrogenase; D010732:Phosphofructokinase-1