Urease activity of adherent bacteria and rumen fluid bacteria.
In experiments on six sheep fed on a low nitrogen diet (3.7 g N/day), urease (EC 3.5.1.5) activity (nkat X mg-1 bacterial dry weight) 3 h after feeding was found to be highest in the bacteria adhering to the rumen wall (13.25 +/- 2.10), lower in the rumen fluid bacteria (8.96 +/- 1.35) and lowest in the bacteria adhering to feed particles in the rumen (5.69 +/- 2.13). The urease activity of bacteria adhering to the rumen wall and of the rumen fluid bacteria of six sheep fed on a high nitrogen diet (21 g N/day) was significantly lower than in sheep with a low N intake and in both cases was roughly the same (3.81 +/- 1.37 and 3.76 +/- 1.02 respectively); it was lowest in bacteria adhering to feed particles in the rumen (1.92 +/- 0.90). It is concluded from the results that the urease activity of rumen fluid bacteria and of bacteria adhering to the rumen wall and to feed particles in the rumen is different and that it falls significantly in the presence of a high nitrogen intake. From the relatively high ureolytic activity of bacteria adhering to the rumen wall in the presence of a low nitrogen intake it is assumed that this is one of the partial mechanisms of the hydrolysis of blood urea entering the rumen across the rumen wall and of its reutilization in the rumen-liver nitrogen cycle in ruminants.
D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000201:enzymology*; D001422:Bacterial Adhesion; D001826:Body Fluids / Q000201:enzymology*; D004044:Dietary Proteins / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology; D012417:Rumen / Q000201:enzymology*; D012756:Sheep; D014510:Urease / Q000378:metabolism*
D004044:Dietary Proteins; D014510:Urease; D009584:Nitrogen