title: Suppression of antigraft immunity by preimmunization. III. Characterization of suppressor T cells involved in suppression of the efferent phase of DTH against alloantigens.
abstract: Suppressor T (Ts) cells that can suppress delayed type hypersensitivity (DTH) against histocompatibility (H) antigens can be isolated from spleen and lymph nodes a few days after i.v. immunization of mice with irradiated allogeneic spleen cells. In this paper we investigated the suppression of the efferent phase of DTH to characterize the Ts cells involved, and to compare them with the afferent phase Ts cells that have been characterized in a previous paper of this series. The DTH against third party alloantigens that were not used for the i.v. suppressive immunization could be suppressed by presenting the third party alloantigens together with the original alloantigens in the challenge inoculum for eliciting the DTH reaction. Thus the ultimate suppressive effect by the Ts cells that are active during the efferent phase of DTH is nonspecific. This non-specific suppression of DTH to alloantigens has previously been found for the afferent phase Ts cells as well. For suppression of the efferent phase of DTH to alloantigens, a population of Lyt-1+2+ Ts cells appeared to be essential, just like in the suppression of the afferent phase of DTH to alloantigens. We did not find evidence for the involvement of cyclophosphamide-sensitive auxiliary Ts cells in suppression of the efferent phase of DTH. Also no evidence was found for H-2 or Igh-restricted activation and function of the Ts cells that were active during afferent and efferent phases of the DTH response to H antigens. In view of these similarities between afferent phase and efferent phase Ts cells we conclude that there are no arguments as yet to suppose that there is more than one type of T cells involved in the suppression of the afferent and efferent limb of DTH against H antigens.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D005260:Female; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006789:Host vs Graft Reaction; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007116:Immunization, Passive / Q000379:methods; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007165:Immunosuppression Therapy / Q000379:methods; D007275:Injections, Intravenous; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology / Q000637:transplantation*; D013997:Time Factors
chemical_list: D000954:Antigens, Surface; D006183:H-2 Antigens; D007143:Immunoglobulin Heavy Chains