[Effects of dithiothreitol and thioredoxines on the in vitro activity of enzymes of the urea cycle in rats].
The five urea cycle enzymes were studied in desactivated extracts of rat liver. After reduction by dithiothreitol (DTT) and in presence of Mg2+ ions, thioredoxines isolated from rat liver were able to activate carbamyl phosphate synthetase-I (CPS-I) and argininosuccinate synthetase (ASS) respectively by 468% and by 370%. Thioredoxines were purified from adult rat liver and an antiserum was raised to these proteins. After immunologic quantitation, their level in adult rat was 0.103 mg/g liver.
D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D007158:Immunologic Techniques; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013879:Thioredoxins / Q000494:pharmacology*; D014508:Urea / Q000378:metabolism*
D001426:Bacterial Proteins; D013879:Thioredoxins; D014508:Urea; D008274:Magnesium; D004229:Dithiothreitol
Effets du dithiothréitol et des thiorédoxines sur l'activité, in vitro, des enzymes du cycle de l'urée chez le rat.