I-J-positive cloned macrophages as accessory cells for the induction of suppressor T cells in vitro.
I-A+/I-J+ cloned macrophages, SL-1, played the role of APC in the in vitro induction of Ts against DTH to BCG. By treating SL-1 cells with various antibodies, it was shown that I-J antigens on SL-1 cells are essential for Ts induction, but not for the effector cell induction for DTH. Ia-negative cloned macrophages, SL-4, did not show any APC activity either in suppressor or effector cell induction. The precursors of the Ts were also I-J-positive, and I-J restriction resided between T cells and macrophages in the Ts induction. Thus, it is suggested that the pre-Ts recognizes the antigenic determinants of BCG presented on the APC in association with the I-J antigen and differentiates into the Ts. This pathway seems analogous to that of helper or effector T induction, where the antigenic determinant is recognized by a T cell in association with the I-A antigen on APC.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001500:BCG Vaccine / Q000276:immunology; D006649:Histocompatibility Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000145:classification / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology*; D014373:Tuberculin / Q000008:administration & dosage
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001500:BCG Vaccine; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; C043911:I-J-antigen; D014373:Tuberculin