Release of atrial natriuretic factor. Effects of repetitive stretch and temperature.
The effect of continuous and repetitive mechanical stretch on release of immunoreactive atrial natriuretic factor (IR-ANF) was studied in isolated rat atria distended by inflation of miniature balloon catheters. After 10 min incubation, the concentration of IR-ANF in the medium increased in proportion to the degree of continuous atrial stretch. Repetitive atrial expansion induced a rate dependent increase in release of IR-ANF which at the highest rate tested (89 inflations/min) was sixfold greater than that observed with continuous maximal expansion. Comparison of the amount of IR-ANF released from the right or left atrium separately revealed that during continuous and repetitive stretch, both atria contribute equally to the total amount of release IR-ANF. A reduction of bath temperature (37 degrees to 21.5 degrees C) decreased both basal- and stretch-induced IR-ANF release approximately 85%. It was concluded that release of atrial natriuretic factor is temperature-dependent and induced by both the extent and rate of atrial stretch.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D010812:Physical Stimulation; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature
D009320:Atrial Natriuretic Factor