title: T cell subsets in the immune rejection of murine heterotopic corneal allografts.
abstract: Using a previously described murine heterotopic corneal allograft model, we examined the roles of delayed-type hypersensitivity (DTH) and cytotoxic T lymphocytes (CTL) in corneal allograft rejection. We have previously shown that normal C57BL/6 mice consistently reject heterotopic corneal allografts within 14 days of grafting. These hosts develop antigen-specific CTL responses but no evidence of DTH reactivity. The absence of DTH suggested that this T cell subset was unnecessary for corneal allograft rejection. The present studies using T cell-deficient mice selectively reconstituted with specific T cell subsets confirmed this suspicion. T cell-deficient (i.e., adult thymectomized, lethally irradiated, bone marrow-reconstituted = ATXBM) C57BL/6 mice were selectively reconstituted with the following categories of syngeneic lymph node cells (LNC): BALB/c skin-immune LNC treated with anti-Lyt 1 antibody + complement; BALB/c skin- or cornea-immune LNC treated with anti-Lyt 2 antibody + complement; or BALB/c cornea- or skin-immune LNC not treated with antibody. ATXBM mice reconstituted with syngeneic Lyt 1-depleted, BALB/c skin-immunized LNC failed to develop DTH, yet rapidly rejected BALB/c corneal allografts. Similarly, ATXBM mice reconstituted with Lyt 1-depleted cornea-immune LNC did not demonstrate DTH responses but were able to reject 100% of the corneal allografts in an accelerated fashion. By contrast, corneal allograft rejection was significantly delayed in ATXBM mice reconstituted with cornea-immune LNC partially depleted of Lyt 2+ T cells. Collectively, the results indicate that: heterotopic corneal allografts can be rejected in the absence of DTH; heterotopic corneal allografts fail to induce allospecific DTH; and partial depletion of Lyt 2+ CTL leads to a prolongation of heterotopic corneal allograft survival. Thus, the primary T cell-dependent immune effector elements responsible for heterotopic corneal allograft rejection appears to reside in the cytolytic T lymphocyte population.
mesh_terms: D000818:Animals; D003315:Cornea / Q000276:immunology*; D016039:Corneal Transplantation; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular; D008212:Lymphocyte Depletion; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012867:Skin / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014184:Transplantation, Homologous