[Modification of the viral immunoassay for determining ultramicro-quantities of antigens].
A technique for ultramicro-quantitation of antigens is suggested. It is based on the antigen isolation by immunosorption and a subsequent assay using phage-antigen conjugate (modification of viral immunoassay). Antibody Fab' fragments were bound to both the immunosorbent and the conjugate through their terminal thio groups. The technique sensitivity was one-two orders higher than that of ELISA for the corresponding antigens.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D010584:Bacteriophage phi X 174 / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D007163:Immunosorbent Techniques; D051381:Rats
D000941:Antigens
Modifikatsiia viroimmunotesta dlia opredeleniia ul'tramikrokolichestv antigenov.