Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes.
Two cDNA clones corresponding to genes that are induced at least 10-fold in peripheral human blood leukocytes by staphylococcal enterotoxin A were isolated and sequenced. Clone 1-3E encodes a 247-residue protein that comprises a putative signal sequence, and resembles a serine protease; the cognate mRNA is expressed in T lymphocyte clones but in none of the other human cell lines tested. The deduced protein sequence is most closely related (68% homology) to that of the postulated protease CCPI from activated murine cytotoxic T lymphocytes and to that of rat mast cell protease II (47% homology). The other cDNA, 3-10C, encodes a protein of 99 residues that resembles human beta-thromboglobulin (42% homology); the cognate mRNA was also found in SEA-stimulated U937 cells, a histiocytic lymphoma-derived cell line.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics*; D004768:Enterotoxins / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D007962:Leukocytes / Q000201:enzymology / Q000502:physiology*; D008934:Mitogens; D012333:RNA, Messenger / Q000235:genetics; D012697:Serine Endopeptidases; D013601:T-Lymphocytes / Q000502:physiology; D001620:beta-Thromboglobulin / Q000235:genetics*
D004768:Enterotoxins; D008934:Mitogens; D012333:RNA, Messenger; D001620:beta-Thromboglobulin; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma; D004247:DNA; D010450:Endopeptidases; D012697:Serine Endopeptidases