Effect of poly(adenosinediphosphoribose) synthesis inhibitors and structurally related compounds on radiation-induced G2 arrest.
A variety of poly(adenosinediphosphoribose) p(ADPR) synthesis inhibitors, structurally related compounds with no inhibitory activity, and agents which reduce radiation-induced G2 arrest, were tested for the concentration dependence of their effect on (a) CHO cell progression to mitosis, (b) the duration of G2 arrest in X-irradiated CHO cells and (c) [14C]NAD incorporation in permeabilized CHO cells, as a measure of p(ADPR) synthetase activity. Caffeine and nicotinamide uptake by viable cells was also measured. The concentration dependencies for reduction of radiation-induced G2 arrest and for p(ADPR) synthesis inhibition were markedly disparate, although all of the active inhibitors of p(ADPR) synthesis did reduce the duration of radiation-induced G2 arrest to some extent. These data indicate that p(ADPR) synthesis is not a requirement for the induction of G2 arrest by ionizing radiation.
D062365:Aminobenzoates / Q000494:pharmacology; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D003839:Deoxyadenosines / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects*; D009525:Niacin / Q000494:pharmacology; D009536:Niacinamide / Q000494:pharmacology; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D013806:Theophylline / Q000494:pharmacology; D062368:meta-Aminobenzoates
D062365:Aminobenzoates; D001549:Benzamides; D003839:Deoxyadenosines; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D062368:meta-Aminobenzoates; D009536:Niacinamide; D009525:Niacin; D002110:Caffeine; C037689:benzamide; C025160:3-aminobenzamide; D013806:Theophylline; C044855:3-aminobenzoic acid