Neutrophil activation detected by monoclonal antibodies.
Monoclonal antibodies have been produced against three neutrophil-associated membrane proteins (p 90, p 170, and p 70) expressed at different maturation stages of the cells. The reactivity of the antibodies against p 90 (B13.9) and p 170 (CLB-gran 10), as measured by quantitative flow cytofluorometry, increased after stimulation of the neutrophils by the calcium ionophore A23187, by phorbol myristate acetate, or by the chemoattractant formylmethionyl-leucyl-phenylalanine in combination with cytochalasin B. This increase is regulated independently of the simultaneously increased expression of the C3bi receptor, because neutrophils of a patient deficient for the C3bi receptor showed a normal increase in membrane expression of p 90 and p 170. Neutrophil cytoplasts were not inducible to increased membrane expression, suggesting that the cytoplasts lack the internal pool of these proteins. The reactivity of the antibody against p 70 (CLB-gram 5) was not affected by activation. The antibodies B13.9 and CLB-gran 10 may be useful to detect neutrophil activation.
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000943:Antigens, Differentiation; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification; D001399:Azure Stains; D002634:Chemotaxis, Leukocyte; D003594:Cytoplasmic Granules / Q000032:analysis / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006410:Hematopoiesis; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D011233:Precipitin Tests; D011951:Receptors, Complement / Q000378:metabolism; D017463:Receptors, Complement 3b
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D001399:Azure Stains; D008565:Membrane Proteins; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; C046168:neutrophil differentiation antigens