title: Characterization of helper T cells induced by the type 2 antigen polyvinylpyrrolidone.
abstract: Type 2 antigens are usually unable to prime for IgG memory responses or to activate helper T cells (TH) necessary for memory B cell generation. Previous studies from this laboratory have established that low doses (0.0025 microgram) of polyvinylpyrrolidone (PVP) or a T-dependent form of PVP, PVP-coupled horse red blood cells (PVP-HRBC) can activate PVP-specific TH. The present study was undertaken in order to determine some of the characteristics of the TH activated by PVP and to compare their properties with those of classical TH1 and of TH2 cells described in many T-dependent systems. TH activated with either 0.0025 microgram of PVP or PVP-HRBC were characterized with respect to cell surface antigens, Igh restriction and generation in mice expressing an X-linked immune defect (xid mice). PVP-specific TH are similar to TH1 cells in that they are required for the production of IgG subclasses absent in primary responses and have the Lyt-1+, L3T4+, I-J-surface phenotype. These TH may not be identical with TH1 cells, however, since they are I-A+ and Igh restricted. PVP-specific TH can be generated in xid mice which do not produce antibody in a primary anti-PVP response and do not develop a memory response to PVP, regardless of whether it is presented as a type 2 or T-dependent antigen.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000637:transplantation; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000145:classification; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology; D007156:Immunologic Memory; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D008817:Mice, Mutant Strains / Q000276:immunology; D010641:Phenotype; D011205:Povidone / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification* / Q000187:drug effects / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D011205:Povidone