Effector functions and specificities of normal murine T cells stimulated by syngeneic blasts.
Large numbers of syngeneically stimulated T cells are easily obtained by stimulation of splenic T cell populations with irradiated lipopolysaccharide (LPS) blasts. Thus, proliferative T cell responses were obtained after stimulation with allogeneic LPS blasts, concanavalin A (Con A) blasts, normal spleen cells or peritoneal macrophages while only LPS and Con A blasts were competent syngeneic stimulators. The cytolytic activity of T cells stimulated with allogeneic LPS blasts was H-2 specific, while that of T cells stimulated with syngeneic LPS blasts was often completely nonspecific, and higher for LPS than for Con A blast target cells. In other experiments, "self" H-2K- and I-A-specific cytotoxic T cells were obtained, without apparent reasons for the development of either type of cytolytic cells. Stimulation of Lyt-2- T cells with syngeneic LPS blasts led to the generation of T helper effector cells which induced normal B cell proliferation in an H-2 restricted manner. This restriction was over-come, the proliferative responses were augmented and plaque-forming cell responses generated, through addition of Con A to the cultures. The results imply the ability of B blasts to induce stimulation of syngeneic T cells, and generation of effector cells displaying cytolytic and helper activity. This phenomenon might play a role in the regulation of the immune system.
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006183:H-2 Antigens / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000950:Antigens, Ly; D006183:H-2 Antigens; D008070:Lipopolysaccharides; D003208:Concanavalin A