[Effect of n-chloromercuribenzoate and albumin on Mg2+, Ca2+-ATPase activity of plasma membranes of smooth muscle cells].
It is shown that Mg2+, Ca2+-ATPase activity of plasma membrane fragments from the rat small intestine myocytes is inhibited by p-chloromercuribenzoate and dithionitrobenzoate (50 and 90%, respectively). The effect of p-chloromercuribenzoate inhibition is removed by serum albumin promoting a rise in the ATPase activity of the plasma membranes and shifting the temperature maximum point up to 32 degrees C (in the norm the maximum is observed at 36 degrees C). According to the data presented, albumin changes the composition and phase state of the lipid surrounding of the membrane enzymes.
D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D002729:Chloromercuribenzoates / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D009130:Muscle, Smooth / Q000201:enzymology*; D011817:Rabbits; D013696:Temperature
D000418:Albumins; D002729:Chloromercuribenzoates; D002784:Cholesterol; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases
Vliianie n-khlormerkuribenzoata i al'bumina na Mg2+, Ca2+-ATP-aznuiu aktivnost' plazmaticheskikh membran gladkomyshechnykh kletok.