title: Dissociation of NS5 from cell fractions containing West Nile virus-specific polymerase activity.
abstract: West Nile virus replication complexes were partially purified from cytoplasmic extracts of virus-infected cells by centrifugation through a 20% glycerol cushion. Numerous cell proteins, as well as the largest nonstructural protein, NS5, were separated from the replication complexes without significant loss of in vitro West Nile virus polymerase activity.
mesh_terms: D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism*; D014779:Virus Replication; D014902:West Nile virus / Q000201:enzymology* / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases