Hematoporphyrin derivative fluorescence: photographic techniques for the localization of malignant tissue.
Three simple methods for the detection of malignancy by the photography of hematoporphyrin derivative (HPD) fluorescence are presented. Two methods employ a single lens reflex camera with macrolens and Kodak high-speed Ektachrome (EL 400) film. Both employ a Corning Glass #3482 filter as a barrier on the lens. In method I, a single Kodak Wratten #39 filter is placed over a fluorescent "black light" source (two General Electric F20T12BL 20-W bulbs). In method II, twin electronic flash units (5,500 BCPS unfiltered) with a double thickness of Kodak Wratten #39 filter over them provide the light of excitation. Method III employs an adapted Polaroid SLR 680E Sun camera with two electronic flash units (5,500 BCPS, total unfiltered) rigged with a slave trigger. A Corning Glass #3482 filter is placed on the lens to act as a barrier, and two filters of Kodak Wratten #39 glass are placed on each flash unit. Photographs are taken in a darkened room.
D000818:Animals; D005260:Female; D005453:Fluorescence; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins; D008325:Mammary Neoplasms, Experimental / Q000175:diagnosis*; D010781:Photography / Q000379:methods*; D011838:Radiation-Sensitizing Agents; D051381:Rats; D011916:Rats, Inbred F344
D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative