Conversion of androstenedione to 3 beta-hydroxy-5-androsten-17-one and 3 beta-hydroxy-4-androsten-17-one by the testicular microsomal fraction of Sprague-Dawley rats.
When androstenedione was incubated with testicular microsomes of Sprague-Dawley rats in the presence of reduced nicotinamide-adenine dinucleotide (NADH), unknown metabolites were produced, in addition to testosterone and 7 alpha-hydroxyandrostenedione. The metabolites were identified as 3 beta-hydroxy-4-androsten-17-one and 3 beta-hydroxy-5-androsten-17-one (3:1) by biochemical and radiochemical methods. These results confirmed the occurrence of the reverse reactions from androstenedione to 3 beta-hydroxy-4-androsten-17-one and 3 beta-hydroxy-5-androsten-17-one catalyzed by the 3 beta-hydroxysteroid dehydrogenase and 5-ene-4-ene isomerase in the microsomal fraction of Sprague-Dawley rat testes.
D000107:Acetylation; D000332:Aerobiosis; D000693:Anaerobiosis; D000735:Androstenedione / Q000378:metabolism*; D000737:Androstenols / Q000378:metabolism; D000738:Androsterone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D000818:Animals; D002855:Chromatography, Thin Layer; D003460:Crystallization; D003687:Dehydroepiandrosterone / Q000096:biosynthesis*; D008297:Male; D008861:Microsomes / Q000378:metabolism; D051381:Rats; D013737:Testis / Q000378:metabolism*
D000737:Androstenols; C047746:3-hydroxy-4-androsten-17-one; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D000738:Androsterone