title: Decrease in tumor-cell attachment and in a 140-kDa fibronectin receptor correlate with greater expression of multiple 34-kDa surface proteins and cytoplasmic 54-kDa components.
abstract: B16 melanoma cells attach to matrix-bound fibronectin but fail to adhere to albumin-coated surfaces supplemented with soluble fibronectin. Attachment to substratum is also decreased in the presence of an adhesion-disrupting antibody, or when cells are seeded on substrates poorly adhesive for these cells, such as collagen gels. We have now investigated some of the more general adhesion-related alterations that occur between flattened and poorly attached cells. Immune blots of octylglucoside extracts with the adhesion-disrupting IgG revealed a 140-kDa component in flattened cells, in contrast to the increased detection of a 54-kDa species in a comparable assay with rounded cells. Surface iodination also showed a decreased external exposure of a 140-kDa fibronectin binding species and an increased labelling in multiple 34-kDa protein species, in cells with decreased attachment to substratum. Analysis of 35S-methionine-labelled cell aggregates cultured on collagen gels also revealed a decrease in the 140-kDa region and a greater labelling of multiple 54-kDa components, compared to the same cells flattened on fibronectin. A change in 54- and 34-kDa species was also seen in matrix-associated components of rounded cells that failed to attach with soluble fibronectin. Since the 34-kDa species increase in poorly adherent cells is mainly detected by iodination, and the 54-kDa species increase in the same cells is partly associated with the corresponding detergent-insoluble matrices, we propose that these 2 novel proteins may relate to cell rounding, through a transmembrane modulation involving both surface membrane and cytoskeletal structures.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002448:Cell Adhesion; D002449:Cell Aggregation; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000032:analysis*; D008546:Melanoma, Experimental / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008970:Molecular Weight; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000032:analysis*
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D005353:Fibronectins; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic