title: Possible role of ADP-ribosylation of adenovirus core proteins in virus infection.
abstract: We have investigated the role of poly(ADP)-ribosylation of adenoviral proteins in virus infection. Viral core proteins V and the precursor to protein VII were shown to be in vivo and in vitro acceptors of ADP-ribose. In vivo ADP-ribosylation was restricted to viral proteins as the histones were not labeled during the late phase of infection. The ADP-ribosylated core proteins were assembled into mature virus particles. In vitro ADP-ribosylation of adenoviral core proteins performed with purified poly(ADP-ribose) polymerase led to relaxation of the chromatin structure of both ts1 and wild type pyridine cores and pentonless particles and triggered the complete dissociation of wild type particles. A critical role for poly(ADP)-ribosylation in virus infection was confirmed by measuring the effect of the inhibitors 3-aminobenzamide and nicotinamide on virus particle yield and infectivity. Both inhibitors depressed particle yield by up to 9-fold, but infectivity was reduced by up to 10(4)-fold. These results suggest that ADP-ribosylation of adenovirus core proteins may have a role in virus decapsidation.
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000256:Adenoviridae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D001549:Benzamides / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008854:Microscopy, Electron; D009536:Niacinamide / Q000494:pharmacology; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D014758:Viral Core Proteins / Q000032:analysis / Q000378:metabolism*; D014771:Virion / Q000032:analysis / Q000648:ultrastructure
chemical_list: D001549:Benzamides; D002843:Chromatin; D004279:DNA, Viral; D009702:Nucleoside Diphosphate Sugars; D014758:Viral Core Proteins; D000246:Adenosine Diphosphate Ribose; D009536:Niacinamide; C025160:3-aminobenzamide