title: Suppression of the cytotoxic T-lymphocyte response in mice by pertussis toxin.
abstract: Pertussis toxin (PT), the major toxin produced by Bordetella pertussis, has been reported both to enhance and to suppress immune responsiveness. These findings suggested that PT contributes to the virulence of B. pertussis through mechanisms involving immune regulation. We report that PT suppressed both the primary and the secondary cytotoxic T-lymphocyte (CTL) responses of mouse spleen cells cultured against two different allogeneic stimulator spleen cells in vitro. This suppression was dependent on the dose of PT used. PT must be present during the initial stages (within the first 24 hr) of CTL generation. Soluble factor(s) obtained from spleen cells preexposed to PT did not suppress the CTL response. Suppression of the CTL response observed was not due to depletion of the antigen by PT. The cytotoxic activity of CTL clones could not be suppressed by PT. The analysis of responder spleen cells, fractionated by anti-immunoglobulin panning techniques, provided evidence that L3T4-, Lyt 2+ cells mediate the PT-induced immunosuppression. We propose that suppression of the CTL response by PT is generated through the activation of L3T4-, Lyt 2+ suppressor T lymphocytes.
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D003864:Depression, Chemical; D007700:Kinetics; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D037342:Pertussis Toxin; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D000941:Antigens; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin