Identification of nonecdysteroid steroids in hemolymph of both male and female Astacus leptodactylus (Crustacea) by gas chromatography-mass spectrometry.
The O-pentafluorobenzyloxime (OPFB)-heptafluorobutyrylester (HFB) derivatives of hemolymph steroid extracts from both male and female Astacus leptodactylus were subjected to negative ion chemical ionization and capillary gas chromatography-mass spectrometry (NCI/GC-MS). Five nonecdysteroid steroids, namely pregnenolone, 17 alpha-hydroxypregnenolone, testosterone, cholesterol, and 6 beta-hydroxyprogesterone were identified. With selected ion monitoring (SIM), indications were found for the presence of four more steroids: androstenedione, 5 alpha-dihydrotestosterone, 11-ketotestosterone, and 11 beta-hydroxytestosterone. The presence of 6 beta-hydroxyprogesterone could only be demonstrated in the female hemolymph. With the technique used, dehydroepiandrosterone, progesterone, 17 alpha-hydroxyprogesterone, and estrogens could not be detected in male or female hemolymph extracts.
D006907:17-alpha-Hydroxypregnenolone / Q000097:blood; D000735:Androstenedione / Q000097:blood; D000818:Animals; D002784:Cholesterol / Q000097:blood; D003445:Crustacea / Q000032:analysis*; D003687:Dehydroepiandrosterone / Q000097:blood; D013196:Dihydrotestosterone / Q000097:blood; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006458:Hemolymph / Q000032:analysis*; D006908:Hydroxyprogesterones / Q000097:blood; D006915:Hydroxytestosterones / Q000097:blood; D008297:Male; D011284:Pregnenolone / Q000097:blood; D013256:Steroids / Q000032:analysis*; D013739:Testosterone / Q000031:analogs & derivatives / Q000097:blood
D006908:Hydroxyprogesterones; D006915:Hydroxytestosterones; D013256:Steroids; D013196:Dihydrotestosterone; C037914:11-hydroxytestosterone; D006907:17-alpha-Hydroxypregnenolone; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; C048534:6-hydroxyprogesterone; D011284:Pregnenolone; D002784:Cholesterol; C003600:11-ketotestosterone