Influence of carriers on the development and localization of anti-2,4,6-trinitrophenyl (TNP) antibody-forming cells in the murine spleen. II. Suppressed antibody response to TNP-Ficoll after elimination of marginal zone cells.
The macrophages in the marginal zones in mice spleens were eliminated by i.v. injection of dichloromethylene diphosphonate encapsulated in liposomes. After immunization with a thymus-dependent (trinitrophenylated keyhole limpet hemocyanin; TNP-KLH) or thymus-independent type 1 (TNP-lipopolysaccharide) antigen, no differences in antibody responses in treated and untreated mice were observed. However, immunization with a thymus-independent type 2 (TI-2) antigen (TNP-Ficoll) resulted in a strong decrease of the antibody response in macrophage-depleted animals. Anti-TNP serum levels dropped 10-fold and the number of antibody-forming cells in the spleen 30-60-fold. These results confirm the special role of the marginal zone macrophages in the processing of TI-2 antigens, as earlier suggested in splenectomy studies.
D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology*; D004002:Clodronic Acid / Q000008:administration & dosage; D005362:Ficoll / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007108:Immune Tolerance; D008070:Lipopolysaccharides / Q000276:immunology; D008081:Liposomes; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D008081:Liposomes; D009578:Nitrobenzenes; D011134:Polysaccharides; D014301:Trinitrobenzenes; D004002:Clodronic Acid; D005362:Ficoll; D006433:Hemocyanins