Identification of 5-hydroxytryptamine binding site subtypes in rat spinal cord.
High-affinity, specific [3H]5-hydroxytryptamine (5-HT) binding was analyzed in rat spinal cord homogenates. Drug competition studies were performed using 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) to compete selectively for 5-HT1A sites, RU 24969 to compete for 5-HT1B sites and mesulergine to compete for 5-HT1C sites. Competition data were analyzed by computer-assisted iterative curve-fitting analysis. The results demonstrate that 5-HT1A, 5-HT1B and 5-HT1C binding sites are present in rat spinal cord. In addition, approximately 33% of total 5-HT1 sites do not appear to represent either 5-HT1A, 5-HT1B or 5-HT1C binding sites. Therefore, 5-HT1D and/or some other 5-HT1 binding site subtype may also be present in rat spinal cord.
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D004873:Ergolines / Q000378:metabolism; D066298:In Vitro Techniques; D007211:Indoles / Q000378:metabolism; D051381:Rats; D011985:Receptors, Serotonin / Q000032:analysis* / Q000378:metabolism; D012701:Serotonin / Q000032:analysis* / Q000378:metabolism; D013116:Spinal Cord / Q000032:analysis* / Q000378:metabolism; D013764:Tetrahydronaphthalenes / Q000378:metabolism
D004873:Ergolines; D007211:Indoles; D011985:Receptors, Serotonin; D013764:Tetrahydronaphthalenes; C025145:5-methoxy 3-(1,2,3,6-tetrahydro-4-pyridinyl)1H indole; D012701:Serotonin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; C031649:mesulergine