Human salivary or plasma aldosterone- and dehydroepiandrosterone-binding glycoprotein induces hypertension in the rat.
In this study we demonstrated the presence of an aldosterone-binding globulin (ABG) in human saliva. Following ultrafiltration and purification of saliva to electrophoretic homogeneity, this thermolabile globulin called ABG-S appeared to be identical to plasma ABG. It was also shown that both purified ABG-S and plasma ABG bind reversibly, with high affinity (Ka = 1.6 X 10(-9) M, Scatchard plot) to dehydroepiandrosterone sulfate (DHEA-S) to binding sites different from those for aldosterone. Following incubation with human urine both ABG and ABG-S were converted quantitatively into corresponding thermostable forms which appeared to be identical with the human urinary ABG homologue namely ABG-TsU. In addition ABG-S showed the same ability to elevate blood pressure when administered to rats as did ABG-TsU. The fact that an endogenous substance with pressor activity might exist in more than one body fluid suggests further its possible participation in the basic mechanisms of blood pressure regulation.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000494:pharmacology; D002352:Carrier Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives / Q000378:metabolism; D019314:Dehydroepiandrosterone Sulfate; D006801:Humans; D006973:Hypertension / Q000209:etiology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000032:analysis; D012712:Serum Globulins
D001798:Blood Proteins; D002352:Carrier Proteins; D012712:Serum Globulins; C488874:aldosterone-binding globulin protein, rat; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate