title: Identification of a subset of normal B cells with a Burkitt's lymphoma (BL)-like phenotype.
abstract: Fresh biopsy cells from cases of Burkitt's lymphoma (BL) display a homogeneous cell surface phenotype. The cells were found to be reactive with the pan B cell marker B1, and consistently co-expressed the BL-associated glycolipid antigen, BLA, and the common acute lymphoblastic leukemia antigen, CALLA, but lacked the B cell "activation" antigens characteristically expressed on EB virus-transformed normal B cells. Microscopic and cell sorter analysis of cells isolated from a series of fresh normal tonsils have identified a subpopulation of normal B cells carrying the same cell surface markers. That BLA and CALLA could be co-expressed on individual B cells was demonstrated by two-color immunofluorescence (IF) of tonsils in suspension, and immunoperoxidase (IP) staining of serial tonsil sections. These BLA+, CALLA+, "activation" antigen- cells were further characterized as B1+, sIgM+, sIgD-, C3d/EB virus receptor+ and were susceptible to virus-induced transformation in vitro. IF studies on Percoll-fractionated tonsillar cell populations and direct examination of IP-stained tonsil semi-thin sections indicated that the BLA+, CALLA+ cells were localized in germinal centers. Their morphological characteristics matched those of BL cells, and their location within germinal centers was consistent both with the known phenotype of germinal center tonsillar B cells and with the description of BL as a proliferation of centroblasts. We suggest that this population of tonsillar germinal center B cells provides the normal counterpart of BL tumor cells.
mesh_terms: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D001402:B-Lymphocytes / Q000145:classification* / Q000276:immunology; D002051:Burkitt Lymphoma / Q000276:immunology / Q000473:pathology*; D002469:Cell Separation; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans; D015260:Neprilysin; D014066:Palatine Tonsil / Q000166:cytology; D010641:Phenotype
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006028:Glycosphingolipids; C018480:ceramide 3; D015260:Neprilysin