A method to exchange alkali light chains on myosin subfragment 1.
Exchange of bound alkali light chains on myosin by free alkali light chains is described. It was found that the yield of hybrid obtained was dependent on the incubation time in 4.7 M NH4Cl at pH 9.5. 60% recovery of S1(A1) from S1(A2) was obtained using only a 2-fold molar excess of A1 over S1(A2).
D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002918:Chymotrypsin; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008722:Methods; D009132:Muscles; D015879:Myosin Subfragments; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D055503:Protein Multimerization; D011817:Rabbits
D000199:Actins; D046911:Macromolecular Substances; D015879:Myosin Subfragments; D010446:Peptide Fragments; D002918:Chymotrypsin; D000251:Adenosine Triphosphatases; D009218:Myosins