Stimulator T-cells and the regulation of growth factors produced in the murine mixed leukocyte reaction.
Previous work had implicated T cells within the stimulating population as having a significant role in the MLR. We now report that depletion of T cells from the stimulators caused a reduction in proliferative response in mixed leukocyte reaction (MLR) by 50% or more. This response could be restored by adding T-enriched cells from the stimulators but not from the responders. During MLR, the T-cells in the stimulating population demonstrated changes characteristic of early activation, namely, increase in RNA synthesis. Stimulator T-cells have been shown to regulate the interleukin 2 (IL 2) production in mixed leukocyte culture (MLC). Depletion of T cells from the stimulators reduced the level of IL 2 in the culture. Addition of IL 2 to the cultures containing T-depleted stimulators reconstituted the proliferative response. Our data suggest that there may be alternative ways of producing IL 2-like growth factors. Depletion of T cells from the stimulators allows the detection of a growth factor which differs from IL 2 in its mode of production. We conclude that optimal proliferation in the MLR requires that the stimulating population contain T cells which regulate the growth factors in the culture.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D006133:Growth Substances / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
D006133:Growth Substances; D007376:Interleukin-2; D008937:Mitomycins; D003208:Concanavalin A; D016685:Mitomycin