Isolation and purification of an antibody to 67-KD calcimedin.
The 67-KD calcimedin is a calcium-binding protein isolated from several muscle tissues. The protein shows apparent Mr of 67,000 by SDS-polyacrylamide gel electrophoresis. An antibody has been prepared by immunizing sheep with the protein purified from chicken gizzard smooth muscle. This antibody recognizes 67-KD calcimedin but not calmodulin, bovine serum albumin, transferrin, or brain p68 calelectrin. The presence of 67-KD calcimedin is demonstrated in the smooth muscle cell lines A10 and DDT1MF-2 as well as in primary cultures of chicken breast and heart muscle, by immunoprecipitation and immunofluorescence. The 67-KD calcimedin, being responsive to calcium, may play a role in calcium-mediated cell regulation. This report identifies several cells that may be useful for further delineation of the cellular role of 67-KD calcimedin.
D000818:Animals; D017302:Annexins; D000906:Antibodies / Q000302:isolation & purification*; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000276:immunology*; D002460:Cell Line; D002645:Chickens; D005455:Fluorescent Antibody Technique; D005895:Gizzard, Avian / Q000032:analysis; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000032:analysis / Q000166:cytology
D017302:Annexins; D000906:Antibodies; D002135:Calcium-Binding Proteins