title: Role of pyocyanin in the acquisition of iron from transferrin.
abstract: Pseudomonas aeruginosa produces a blue pigment called pyocyanin. In the presence of oxidizable substrates, bacteria reduce this pigment to a colorless product, leukopyocyanin. Pyocyanin can also be nonenzymatically reduced by NADH. Leukopyocyanin formed by cell- or NADH-mediated reduction nonenzymatically reduces oxygen or Fe(III). Pyocyanin-dependent iron reduction by whole bacterial cells was measured by the formation of the ferrous-ferrozine complex. In addition, leukopyocyanin reduced chelated Fe(III) including ferric iron in complex with transferrin, the serum iron-binding protein. High-pressure liquid chromatography was used to display the reductive removal of iron from transferrin and the accumulation of iron in the ferrous-ferrozine complex. Pyocyanin stimulated the accumulation of 55Fe from [55Fe]transferrin when it was added to bacteria incubated under low-oxygen conditions. Although bacteria grown in the presence of 100 microM FeCl3 reduced pyocyanin just as rapidly as iron-limited bacteria, these cells did not accumulate iron in the presence or absence of pyocyanin. Therefore, P. aeruginosa participates indiscriminantly in the reduction of pyocyanin, but soluble or available iron generated by the pyocyanin is taken up specifically by iron-limited bacteria.
mesh_terms: D003470:Culture Media; D005290:Ferric Compounds / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism; D009842:Oligopeptides; D010084:Oxidation-Reduction; D010619:Phenazines / Q000502:physiology*; D010636:Phenols / Q000378:metabolism; D010860:Pigments, Biological / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D011710:Pyocyanine / Q000502:physiology*; D017262:Siderophores; D013844:Thiazoles; D014168:Transferrin / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005290:Ferric Compounds; D007502:Iron Chelating Agents; D009842:Oligopeptides; D010619:Phenazines; D010636:Phenols; D010860:Pigments, Biological; D017262:Siderophores; D013844:Thiazoles; D014168:Transferrin; C025316:pyochelin; C042453:pyoverdin; D011710:Pyocyanine; D007501:Iron