title: Heterogeneous immunostaining patterns of follicular dendritic reticulum cells in human lymphoid tissue with selected antibodies reactive with different cell lineages.
abstract: A comparative immunohistologic study of the cell density and distribution pattern of follicular dendritic reticulum cells (DRCs) within their follicular microenvironments (germinal centers and mantle zones) was performed by immunoperoxidase technique with a selected panel of antibodies either operationally specific for DRCs (DRC-1) or reported as having additional immunoreactivity with DRCs (antibodies to B- and T-cells, leukocytes, monocytes/macrophages, desmosomal components, and S-100 protein). Twenty-five biopsy specimens, including reactive lymph nodes and tonsils as well as normal spleen tissue, were analyzed. Serial frozen sections were tested either with single antibodies or paired monoclonal reagents in double-labeling procedures. Our results consistently indicated that DRCs positive for Leu M3 and BA-2 antibodies were confined to the central portion of germinal centers, whereas DRCs immunoreactive for S-100 protein and desmoplakin 1 and 2 were localized mostly in the central and pericentral portion of germinal centers. All the DRCs extending from the central portion of germinal centers to the mantle zones were labeled with DRC-1 and, unexpectedly, with OKB7 antibodies. Other immunostainings, such as those for HLA-DR antigens, common leukocyte antigen, and Leu 3a, were not contributory in defining topographic differences of DRCs within the follicle. The consistent heterogeneity of the labeling patterns appears to suggest a possible in situ immunophenotypic grouping of DRCs, and the concept of their possible heterogeneity appears to be corroborated.
mesh_terms: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000276:immunology; D002452:Cell Count; D003598:Cytoskeletal Proteins; D003713:Dendritic Cells / Q000276:immunology*; D051180:Desmoplakins; D006649:Histocompatibility Antigens / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology; D009418:S100 Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D003598:Cytoskeletal Proteins; C495294:DSP protein, human; D051180:Desmoplakins; D006649:Histocompatibility Antigens; D008562:Membrane Glycoproteins; D009418:S100 Proteins; D017493:Leukocyte Common Antigens