Quantitation of myo-inositol as its hexakis(trifluoroacetyl) derivative with negative ion chemical ionization mass spectrometry.
Highly volatile hexakis(trifluoroacetyl) derivatives of myo-inositol and hexadeutero-myo-inositol have been prepared and analysed by capillary column gas chromatography/mass spectrometry. The electron impact and negative ion (methane) chemical ionization mass spectra of these compounds have been determined. Negative ion chemical ionization mass spectrometric analysis of hexakis(trifluoroacetyl)-myo-inositol achieves a sensitivity one order of magnitude greater than electron impact mass spectrometric analysis of hexakis(trimethylsilyl)-myo-inositol at appropriate selected ions. Stable isotope dilution measurement of myo-inositol versus hexadeutero-myo-inositol employing gas chromatography/negative ion chemical ionization mass spectrometry of the hexakis(trifluoroacetyl) derivatives is demonstrated, and this method is applied to the detection of inositol derived from hydrolysis of water-soluble inositol phosphates obtained from isolated pancreatic islets.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004355:Drug Stability; D005463:Fluoroacetates / Q000032:analysis* / Q000138:chemical synthesis; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D007294:Inositol / Q000031:analogs & derivatives* / Q000032:analysis* / Q000138:chemical synthesis; D007295:Inositol Phosphates / Q000032:analysis; D007515:Islets of Langerhans / Q000032:analysis; D013058:Mass Spectrometry; D051381:Rats
D005463:Fluoroacetates; D007295:Inositol Phosphates; C049466:hexakis(trifluoroacetyl)inositol; D007294:Inositol