title: Differential DNA methylation during the vegetative life cycle of Neurospora crassa.
abstract: Isotope dilution gas chromatography-mass spectrometry analysis of genomic DNAs isolated from the different growth phases of Neurospora crassa revealed significant differences in the amounts of 5-methylcytosine; the mol % of 5-methylcytosine was 0.36 in conidia (asexual spores), 0.40 in conidial germlings cultured for 3 h, 0.24 in mycelial cells that had grown exponentially for 6 and 12 h, and 0.40 in stationary-phase mycelial cells. These results indicate an approximate inverse correlation between the level of C methylation in the DNA and the presumed level of developmentally related transcriptional activity.
mesh_terms: D044503:5-Methylcytosine; D003596:Cytosine / Q000031:analogs & derivatives / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics*; D008401:Gas Chromatography-Mass Spectrometry; D005786:Gene Expression Regulation; D008745:Methylation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development
chemical_list: D004271:DNA, Fungal; D044503:5-Methylcytosine; D003596:Cytosine