Screening cDNA expression libraries with monoclonal and polyclonal antibodies using an amplified biotin-avidin-peroxidase technique.
A chromogenic method using biotinylated secondary antibodies and peroxidase-coupled avidin for screening cDNA expression libraries is described. This method offers increased sensitivity over peroxidase-coupled secondary antibodies and rapid processing of samples, and avoids preparation and handling of radioactive materials. All materials for the chromogenic assay are available commercially and the method offers a fast and easy way to screen lambda and plasmid expression libraries with mono- and polyclonal antibodies.
D000818:Animals; D000911:Antibodies, Monoclonal; D010582:Bacteriophage lambda / Q000032:analysis; D003101:Collodion; D004247:DNA / Q000032:analysis*; D007124:Immunoenzyme Techniques; D051379:Mice; D010957:Plasmids; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D003101:Collodion; D004247:DNA