A human retinoblastoma cell line expressing the common acute lymphoblastic leukemia antigen and displaying an unusual chromosome abnormality.
A new continuous cell line derived from an untreated human retinoblastoma has been established. This cell line, FMC-RB1 is strongly positive for common acute lymphoblastic leukemia antigen and shows a number of ring chromosomes and two marker chromosomes considered to be derivations of chromosome #17; the nonrandom chromosomal changes associated with retinoblastoma, particularly the loss of a chromosome #13 or the deletion of 13q14 was not observed. The establishment of the cell line initially required the presence of bone marrow stromal cells. Morphologically, this cell line grew as a suspension of small round cells in grape-like clusters with periodic "shedding" of single cells. FMC-RB1 could be cloned in soft agar, even in the absence of bone marrow stromal cells as "feeders", making it suitable for a variety of biological studies.
D000951:Antigens, Neoplasm / Q000032:analysis*; D001854:Bone Marrow Cells; D002460:Cell Line; D002675:Child, Preschool; D002869:Chromosome Aberrations; D003470:Culture Media; D006801:Humans; D007621:Karyotyping; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008854:Microscopy, Electron; D015260:Neprilysin; D012175:Retinoblastoma / Q000235:genetics* / Q000276:immunology / Q000648:ultrastructure; D012303:Ring Chromosomes
D000951:Antigens, Neoplasm; D003470:Culture Media; D015260:Neprilysin