A simple method of preparing myosin from porcine aortae.
A simple method for preparing myosin from porcine aortae is described. The procedure yields a highly pure myosin devoid of any obvious contaminants, such as tropomyosin and actin. The proteolysis of the heavy chain, frequently observed in the earlier procedures, is greatly minimized by the use of several protease inhibitors and by reducing the purification time. The procedure therefore can be used to isolate and purify myosin from porcine aortae for studies where the purity and integrity of the heavy chain are highly desirable.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification; D000818:Animals; D001013:Aorta, Thoracic / Q000032:analysis; D008722:Methods; D009131:Muscle, Smooth, Vascular / Q000032:analysis*; D009218:Myosins / Q000302:isolation & purification*; D013552:Swine
D000251:Adenosine Triphosphatases; D009218:Myosins