Selective loss of suppressor T cells in rheumatoid arthritis patients: analysis of peripheral blood lymphocytes by 2-dimensional flow cytometry.
Two-colour flow cytometry analysis of the peripheral blood T cells in 28 patients with rheumatoid arthritis (RA) and healthy individuals showed a significant decrease in the percentage of the suppressor T cells (Leu 2a + Leu 15+) in severely active disease (5.9 +/- 2.1%) as compared to controls (13.4 +/- 3.8%) and active disease (12.9 +/- 5.9%). A significant adverse correlation was also observed between disease activity (Lansbury's index) and the percentage of suppressor T cells. Activated T cells (Leu 4+ HLA-DR+) were detected in a higher percentage and prevalence in the active group. Two-color immunofluorescence of T cell subsets may provide more accurate information concerning the immune imbalance of RA patients than the conventional single-color analysis.
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000150:complications / Q000276:immunology*; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008213:Lymphocyte Activation; D008231:Lymphopenia / Q000209:etiology* / Q000276:immunology; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface