Evidence for bile acid glucosides as normal constituents in human urine.
A glucosyltransferase catalysing formation of bile acid glucosides was recently isolated from human liver microsomes. In order to investigate the potential occurrence of such bile acid derivatives in vivo, a method was devised for their isolation and purification from urine. Conditions were established with the aid of glucosides of radiolabelled, unconjugated glycine and taurine conjugated bile acids prepared enzymatically using human liver microsomes. Analysis by gas chromatography and mass spectrometry of methyl ester trimethylsilyl ether derivatives indicated the excretion of glucosides of nonamidated hyodeoxycholic, chenodeoxycholic, deoxycholic, ursodeoxycholic and cholic acids and of glycine and taurine conjugated chenodeoxycholic and cholic acids. Additional compounds were present giving mass spectral fragmentation patterns typical of di- and trihydroxy bile acid glycosides. Semiquantitative estimates indicated a total daily excretion of about 1 mumol.
D001647:Bile Acids and Salts / Q000652:urine*; D002635:Chenodeoxycholic Acid / Q000652:urine; D019826:Cholic Acid; D002793:Cholic Acids / Q000652:urine; D002845:Chromatography; D003840:Deoxycholic Acid / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D005960:Glucosides / Q000652:urine*; D005964:Glucosyltransferases / Q000378:metabolism; D005998:Glycine / Q000652:urine; D006027:Glycosides / Q000652:urine*; D006801:Humans; D008862:Microsomes, Liver / Q000201:enzymology; D013654:Taurine / Q000652:urine; D014580:Ursodeoxycholic Acid / Q000652:urine
D001647:Bile Acids and Salts; D002793:Cholic Acids; D005960:Glucosides; D006027:Glycosides; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D013654:Taurine; D014580:Ursodeoxycholic Acid; C010471:hyodeoxycholic acid; D005964:Glucosyltransferases; D019826:Cholic Acid; D005998:Glycine