title: NAD+-mediated stimulation of adenylate cyclase in cardiac membranes.
abstract: NAD+ significantly enhances adenylate cyclase activity in crude cardiac membrane preparations. This increase is dose-dependent, does not occur in the presence of nicotinamide, ADP-ribose or NADP+, and can be effected by a 30 min pre-incubation period with NAD+. Time course studies are consistent with an enzymatically mediated modification that used NAD+ as substrate. Furthermore, inhibition of NAD+-mediated activation by arginine suggests that this modulation of cardiac adenylate cyclase is analogous to that catalyzed by endogenous ADP-ribosyl transferases.
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D005576:Colforsin / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008345:Manganese / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology*; D009243:NAD / Q000494:pharmacology*; D009249:NADP / Q000494:pharmacology; D009536:Niacinamide / Q000494:pharmacology; D011817:Rabbits; D012969:Sodium Fluoride / Q000494:pharmacology
chemical_list: D009243:NAD; D005576:Colforsin; D000246:Adenosine Diphosphate Ribose; D009536:Niacinamide; D006165:Guanylyl Imidodiphosphate; D008345:Manganese; D009249:NADP; D012969:Sodium Fluoride; D001120:Arginine; D000262:Adenylyl Cyclases; D007545:Isoproterenol