A quantitative thin layer chromatography method for the analysis of aflatoxins, ochratoxin A, zearalenone, T-2 toxin and sterigmatocystin in foodstuffs.
A simple multimycotoxin analytical method was devised for the assay of 8 mycotoxins in foodstuff samples. The procedure consisted in the extraction of the samples with acetonitrile-4% KCl with the addition of HCl, defating with hexane and final extraction with methylene chloride. The mycotoxins were separated by bidimensional thin layer chromatography. The recovery was higher than 80% for all the mycotoxins. The limits of detection were 1.2 micrograms aflatoxins, 5 micrograms ochratoxin A, 20 micrograms sterigmatocystin, 100 micrograms T-2 toxin and 100 micrograms zearalenone/kg.
D000348:Aflatoxins / Q000032:analysis; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods*; D002523:Edible Grain / Q000032:analysis*; D006801:Humans; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis; D013605:T-2 Toxin / Q000032:analysis; D015025:Zearalenone / Q000032:analysis
D000348:Aflatoxins; D009183:Mycotoxins; D009793:Ochratoxins; D015025:Zearalenone; D013605:T-2 Toxin