Red-cell metabolism in patients with iron deficiency.
18 components of metabolism were determined in the red cells of iron-deficient patients and data were expressed per 10(12) red cells to avoid the complicating effects of hypochromia and microcytosis. Glucose consumption, AMP and ATP, glycolytic intermediates except 2,3-bisphosphoglycerate (2,3-DPG) and phosphoenolpyruvate (PEP), red-cell Na+ and the net passive leakage of Na+ and K+ at 4 degrees C were all normal. Creatine, 6-phospho-D-gluconate: NADP oxidoreductase (6PGD) activity and fresh red-cell K+ were raised, suggestive of a young cell population. However, ATP: D-fructose-6-phosphate 1-phosphotransferase (PFK) activity and ADP were low. An elevated 2,3-DPG level was attributable to the anaemia present but the somewhat raised PEP level is unexplained. It is concluded that red cells in iron deficiency show some characteristics of a young cell population; in other respects they appear normal, but in containing a low PFK activity they are abnormal.
D019794:2,3-Diphosphoglycerate; D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D000747:Anemia, Hypochromic / Q000097:blood* / Q000201:enzymology / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010728:Phosphoenolpyruvate / Q000097:blood; D010732:Phosphofructokinase-1 / Q000097:blood; D010734:Phosphogluconate Dehydrogenase / Q000097:blood; D011188:Potassium / Q000097:blood; D012964:Sodium / Q000097:blood
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D012964:Sodium; D003404:Creatinine; D010734:Phosphogluconate Dehydrogenase; D010732:Phosphofructokinase-1; D011188:Potassium