Nicotinamide deficiency and benzamide-induced sister chromatid exchanges.
Benzamides induce sister chromatid exchanges (SCE) in L1210 cells. This induction is strongly potentiated when the cells are grown in nicotinamide-free medium. There is no dependence on the concentration of bromodeoxyuridine (BrdUrd) except at toxic doses, high enough to inhibit BrdUrd incorporation into the DNA. Nicotinamide starvation by itself does not increase the frequency of SCE markedly. These observations are consistent with the notion that BrdUrd and benzamides induce SCE by different mechanisms. The mode of action of benzamides in inducing SCE is still unclear.
D000818:Animals; D001549:Benzamides / Q000633:toxicity*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004260:DNA Repair; D051379:Mice; D009536:Niacinamide / Q000172:deficiency*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D009536:Niacinamide; C037689:benzamide; D004247:DNA; D001973:Bromodeoxyuridine