Expression of anionic sites at the dermoepibolic junction.
The emergence of anionic sites during basement membrane zone formation was studied using migrating epidermis in organ culture as a model system. Ultrastructural investigations using a strongly cationized probe revealed that the heparitinase-sensitive, anionic sites were formed synchronously with the newly built basal lamina after 7 days in culture.
D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure*; D002465:Cell Movement; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis*; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000096:biosynthesis*; D006801:Humans; D009924:Organ Culture Techniques; D011509:Proteoglycans / Q000096:biosynthesis*
D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D011509:Proteoglycans