title: [Giardia intestinalis: influence of the composition of the osmolarity of the medium on in vitro excystation].
abstract: The in vitro excystation of Giardia intestinalis was studied to make the osmolarity (from 50 to 500 mosmol/l) and the components of growth medium (MCI saline solution, MCII glucose solution, MCIII nutritive solution) varying. The percentage of excystation, the viability and the generation time were determined. Excystation was observed in the saline solution between 100 to 450 mosmol/l after cyst acid pepsin incubation. The trophozoite viability was increased by glucose addition (60 min in MCI; 300 min in MCII). Only a rich medium (MCIII) permitted a generation time from 225 to 425 mosmol/l.
mesh_terms: D000818:Animals; D003470:Culture Media; D005243:Feces / Q000469:parasitology; D005872:Giardia / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D005947:Glucose / Q000494:pharmacology*; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000494:pharmacology*; D014327:Trophoblasts / Q000187:drug effects
chemical_list: D003470:Culture Media; D012965:Sodium Chloride; D005947:Glucose
vernacular_title: Giardia intestinalis: influence de la composition et de l'osmolarité du milieu sur le dékystement in vitro.