Proton-motive-force-dependent step in the pathway to lysis of Escherichia coli induced by bacteriophage phi X174 gene E product.
We examined the cellular effects after the expression of the cloned lysis gene E of bacteriophage phi X174. Chloramphenicol prevented lysis only when added within the first minute of derepression of E synthesis, indicating that a time lag of several minutes exists between the synthesis of the E protein and the onset of cell lysis. Experiments with protonophores showed the existence of a subsequent step dependent on proton motive force at about 3 to 5 min before lysis.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001433:Bacteriolysis / Q000187:drug effects; D010584:Bacteriophage phi X 174 / Q000235:genetics / Q000502:physiology*; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D005814:Genes, Viral; D006863:Hydrogen-Ion Concentration; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D014764:Viral Proteins; D002701:Chloramphenicol; D000255:Adenosine Triphosphate