[The PWM model of the immunoglobulin synthesis capacity of human peripheral blood lymphocytes. II. Modification of the immunofluorescent detection of cytoplasmic immunoglobulins (cIg+)].
To detect intracytoplasmic antibodies after PWM-stimulation of peripheral mononuclear cells we used class specific antisera against IgG, A and M and ascertained the whole number of cIg+-cells by addition. The number of cIg+-cells after culturing 7 days without mitogen was below 1%, with PWM-stimulation 14,7% +/- 8,5 (n = 29, blood donors). We discussed the requirements for the culture conditions and for the immunofluorescence detection.
D001782:Blood Donors; D002478:Cells, Cultured; D003593:Cytoplasm / Q000187:drug effects / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis*; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008954:Models, Biological; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D013997:Time Factors
D007136:Immunoglobulins; D011043:Pokeweed Mitogens
Das PWM-Modell des Immunglobulin-Synthese-Vermögens menschlicher peripherer Blutlymphozyten. II. Modifikation des fluoreszenzoptischen Nachweises zytoplasmatischer Immunglobuline (cIg+).