Acid ATPase from chicken liver lysosomes. III. A metal ion-activated ATPase combines with membranous phosphatidylinositol.
A metal ion-activated acid ATPase was present in chicken liver lysosomes. We used Zn2+ as an activator. Lysosomal extract containing octylglucoside from chicken liver was centrifuged at 100,000 xg for 60 min. The supernatant was analyzed by gel filtration on a Sepharose 6B column. Two peaks of metal ion-activated acid ATPase activities were obtained according to the distribution patterns. Each of the two active fractions was incubated with phosphatidylinositol-specific phospholipase C at 37 degrees C for 60 min. The resulting solution was analyzed by gel filtration on a smaller size column of Sepharose 6B again. Molecular weight of the major peak was altered from approx. 1,600,000 to 130,000, whereas that of the minor one, 700,000, remained unchanged.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D002850:Chromatography, Gel; D004789:Enzyme Activation; D005960:Glucosides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D010738:Type C Phospholipases / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology*
D005960:Glucosides; D008563:Membrane Lipids; D010716:Phosphatidylinositols; C018619:octyl-beta-D-glucoside; D010738:Type C Phospholipases; D000251:Adenosine Triphosphatases; D015032:Zinc