title: Simple methods which maintain the barrier status of specific-pathogen-free animals during experimentation.
abstract: Several simple procedures for experimentation on specific-pathogen-free rats and mice are described which combine the technique of plastic-film-isolator containment and a laminar flow sterile environment. These permit the full range of cellular immunology experiments to be performed without compromising the microbiological barrier.
mesh_terms: D000818:Animals; D005160:Facility Design and Construction; D005856:Germ-Free Life; D006799:Housing, Animal; D008722:Methods; D051379:Mice; D051381:Rats; D013047:Specific Pathogen-Free Organisms