Trichloroethylene inhibits uptake of 3H-5-hydroxytryptamine but not uptake of 3H-zimeldine or 3H-propranolol in isolated perfused rat lungs.
Pulmonary uptake of 5-hydroxytryptamine (5-HT), zimeldine and propranolol were studied using the isolated perfused rat lung model. The 5-HT uptake was found to be attenuated by approximately 50 per cent in comparison to the control, when the lungs were ventilated with air containing 5,000 p.p.m. trichloroethylene. In experiments in which the active uptake of 5-HT was blocked with the selective 5-HT uptake inhibitor zimeldine (5 X 10(-6) M), the uptake of 5-HT decreased by 70 +/- 1.7 per cent (mean +/- S.E.M.). When trichloroethylene (5,000 p.p.m. and 18,000 p.p.m.) was added, no further decrease in uptake was noted. The uptake of 3H-zimeldine (10(-6) M) and 3H-propranolol (10(-6) M) was unaffected by ventilating the lungs with trichloroethylene. It is concluded that trichloroethylene inhibits the active uptake of 5-HT from the pulmonary circulation, but that it has no effect on the uptake of zimeldine or propranolol, which are taken up predominantly by passive diffusion.
D000818:Animals; D004058:Diffusion; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D011433:Propranolol / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D014241:Trichloroethylene / Q000494:pharmacology*; D015031:Zimeldine / Q000378:metabolism
D014241:Trichloroethylene; D012701:Serotonin; D015031:Zimeldine; D011433:Propranolol