title: Metastatic potential of murine B16 melanoma correlates with reduced surface heparan sulfate glycosaminoglycan.
abstract: We studied the relationship between the metastatic potential of murine B16 melanoma cells and their surface expression of heparan sulfate glycosaminoglycan (HS-GAG) by using HepSS-1, a monoclonal antibody specific to HS-GAG. Firstly, among five B16 sublines, those capable of developing lung colonies with high efficiencies, such as B16-F10, had relatively low levels of surface HS-GAG. Secondly, a subline freshly prepared from metastatic lung colonies (F1) displayed a level of surface HS-GAG lower than that of injected B16 cells. Thirdly, in vitro selection of B16 cells with low surface HS-GAG by repeated HepSS-1 staining and cell-sorting resulted in cells with a higher metastatic efficiency than that of the original B16 cells. Collectively, B16 melanoma cells with high metastatic activities seem to have low surface HS-GAG. We also found that there was a positive correlation between the surface level of HS-GAG and the susceptibility to natural killer cells in eight B16 sublines.
mesh_terms: D000818:Animals; D006025:Glycosaminoglycans / Q000032:analysis*; D006497:Heparan Sulfate / Q000032:analysis*; D007694:Killer Cells, Natural / Q000276:immunology; D008175:Lung Neoplasms / Q000032:analysis / Q000556:secondary; D008297:Male; D008546:Melanoma, Experimental / Q000032:analysis* / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis
chemical_list: D006025:Glycosaminoglycans; D006497:Heparan Sulfate