title: Rapid isolation and characterization of hybridization selected recombinants from lambda genomic libraries.
abstract: This paper describes an efficient protocol for the screening of lambda genomic libraries, plaque and DNA purification, and probe characterization by a combination of new and recently described techniques. The protocol has allowed large numbers of human subchromosome-specific probes to be rapidly generated from an EMBL3 library of human-mouse somatic cell hybrid DNA. The protocol affords considerable savings in time and effort over previous procedures.
mesh_terms: D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D051379:Mice; D011995:Recombination, Genetic
chemical_list: D004274:DNA, Recombinant