Influence of Lactobacillus arabinosus on metabolic enzyme activity of methylazoxymethanol (MAM) acetate in gnotobiotic mice.
The role of Lactobacillus arabinosus in the malignant transformation of tumors of the large intestine was investigated in mice. Methylazoxymethanol (MAM) acetate, at a weekly dose of 0.2 mg/10 g body weight, was given to germfree mice and to mice monocontaminated with either L. arabinosus or Escherichia coli. At sacrifice, the activity of non-specific esterase, beta-glucuronidase, and alcohol dehydrogenase within the liver and intestine was examined biochemically and histochemically. Non-specific esterase activity in the liver and large intestine was significantly higher in L. arabinosus mice than in the other 2 groups. Also, beta-glucuronidase activity in the large intestine and alcohol dehydrogenase activity in the liver were significantly greater in L. arabinosus mice than in the other groups. Esterase was localized in the mitochondria and absorptive granules within the mucosal epithelium of the large intestine. An apparent increase in the number of certain organelles was observed in the L. arabinosus mice, compared with the other groups. These results suggest that L. arabinosus plays an important role in MAM acetate tumorigenesis and malignant transformation.
D000284:Administration, Oral; D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D004950:Esterases / Q000378:metabolism; D005856:Germ-Free Life / Q000187:drug effects*; D005966:Glucuronidase / Q000378:metabolism; D007414:Intestinal Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D007420:Intestine, Large / Q000201:enzymology / Q000648:ultrastructure; D007421:Intestine, Small / Q000201:enzymology / Q000648:ultrastructure; D007778:Lactobacillus / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008746:Methylazoxymethanol Acetate / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure
D001391:Azo Compounds; D008746:Methylazoxymethanol Acetate; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D004950:Esterases; D005966:Glucuronidase; C030667:methylazoxymethanol