A microassay for studies of the interaction between Fc receptors and low affinity ligands.
A microassay using 10 microliter of a conjugate of rat IgG and Sepharose was developed for the study of the interaction between the solubilized receptors for IgE of rat basophilic leukemia (RBL) cells, designated H and R, and a variety of low affinity immunoglobulins. Rat IgG2a was used as the model of a low affinity ligand by serving as an inhibitor of the interaction between IgG-Sepharose and the receptors for IgE. Ligand concentrations 2.24 X 10(-7) to 5.37 X 10(-5) M were used along with detergent extracts of 2 X 10(5) RBL cells.
D000818:Animals; D001345:Autoradiography; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G / Q000145:classification / Q000494:pharmacology; D008832:Microchemistry / Q000379:methods*; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D011961:Receptors, Fc / Q000302:isolation & purification* / Q000378:metabolism; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000378:metabolism; D012685:Sepharose / Q000031:analogs & derivatives / Q000494:pharmacology
D007074:Immunoglobulin G; D011961:Receptors, Fc; D017455:Receptors, IgE; D011971:Receptors, Immunologic; C035771:Sepharose CL 4B; D012685:Sepharose