Separation and quantification of choline and acetylcholine by thermospray liquid chromatography/mass spectrometry.
The development of sensitive analytical techniques for use in the identification and qualification of molecules of biological origin remains a constant challenge. Often the compounds of interest are present in a complex mixture and require extensive sample preparation prior to analysis. Thermospray liquid chromatography/mass spectrometry (LC/MS) has been shown to be a method which allows for both separation of target molecules from complex mixtures and sensitive specific detection using a mass spectrometer. In this work thermospray LC/MS is used for the direct detection of intact, underivatized choline and acetylcholine from mouse brain homogenate. The results of our analysis corroborate previous analyses using a variety of indirect measurement techniques and thereby show that this simple direct analysis has wide potential applicability.
D000109:Acetylcholine / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D001923:Brain Chemistry; D002794:Choline / Q000032:analysis* / Q000302:isolation & purification; D002853:Chromatography, Liquid; D008297:Male; D013058:Mass Spectrometry; D051379:Mice
D002794:Choline; D000109:Acetylcholine