D1-dopamine receptor activation mediates [3H]acetylcholine release from rabbit retina.
Dopamine and dopamine receptor agonists (0.1 microM-10 mM) evoked in a concentration-dependent manner, calcium-dependent release of [3H]acetylcholine from the rabbit retina labeled in vitro with [3H]choline. This stimulatory effect of dopamine was antagonized by SCH23390 and by alpha-flupenthixol, but not by S-sulpiride or propranolol. Increases in intracellular cyclic AMP by forskolin, 3-isobutyl-1-methylxanthine and 8-bromo-cyclic AMP also evoked calcium-dependent release of [3H]acetylcholine. These results indicate that activation of a receptor with the biochemical and pharmacological characteristics of a D1-dopamine receptor mediates calcium-dependent release of acetylcholine from rabbit retina, possibly through a cyclic AMP-dependent process.
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D004298:Dopamine / Q000494:pharmacology; D009627:Nomifensine / Q000494:pharmacology; D011817:Rabbits; D011954:Receptors, Dopamine / Q000187:drug effects / Q000502:physiology*; D017447:Receptors, Dopamine D1; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D009627:Nomifensine; D013779:Tetrodotoxin; D000109:Acetylcholine; D004298:Dopamine