title: Affi-gel blue treatment simplifies the protein composition of sarcoplasmic reticulum vesicles.
abstract: Sarcoplasmic reticulum vesicles isolated by conventional techniques usually contain, in addition to the recognized sarcoplasmic reticulum components, several other proteins (phosphorylase, myosin, glyceraldehyde-3-phosphate dehydrogenase, etc.) in variable amounts; these proteins complicate the interpretation of chemical modification data. Incubation of sarcoplasmic reticulum vesicles with Affi-Gel blue particles for 1-4 h at 2 degrees C, followed by sedimentation of the Affi-Gel in a clinical centrifuge, simplifies the protein composition by selective adsorption of the accessory proteins, and improves the consistency of the preparations. The Affi-Gel blue treatment is recommended as part of the standard procedure for the isolation of sarcoplasmic reticulum vesicles.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000032:analysis; D002458:Cell Fractionation / Q000379:methods*; D066298:In Vitro Techniques; D009124:Muscle Proteins / Q000032:analysis*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000032:analysis* / Q000378:metabolism; D014227:Triazines
chemical_list: D009124:Muscle Proteins; D014227:Triazines; C012842:Cibacron Blue F 3GA; D000252:Calcium-Transporting ATPases; D002118:Calcium