Ir gene influence on DNP-specific B-memory-cell formation.
It is well known that Ir genes control the antibody production. To investigate whether they also influence B-memory-cell generation, CBA mice (nonresponders) were primed with dinitrophenyl-poly-(L-tyr,L-glu)-poly-(D,L-ala)-poly-(L-lys)-DNP-TGAL conjugate. At the same time animals were injected with ovalbumin (OVA) to activate OVA-specific T helpers. Two to four weeks later animals were challenged with DNP-OVA conjugate and the number of IgG-producing B cells was determined. The data presented indicate that carrier-specific MHC-restricted T helpers are not required for B-memory-cell generation. It is concluded that the defect of IgG response to DNP-TGAL in CBA mice is caused by a block in the maturation of memory cells to antibody-producing cells.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D004136:Dinitrobenzenes / Q000276:immunology*; D005802:Genes, MHC Class II; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA / Q000276:immunology; D009578:Nitrobenzenes / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D010455:Peptides; C021759:(T,G)-A-L; D010047:Ovalbumin