Fast atom bombardment/tandem mass spectrometry of physalaemin-like peptides.
Fragment ion spectra obtained from collision-induced decomposition of protonated molecular ions have been used to determine amino acid sequences of several physalaemin-like peptides which were recently purified from rabbit stomach. This technique was chosen because the peptides were available in microgram quantities and were anticipated to contain pyroglutamate as the blocked N-terminal residue. Such spectra of several synthetic analogs of the naturally occurring peptides were obtained and analyzed to confirm the veracity of this peptide sequencing strategy. In addition, methyl ester derivatives of these synthetic peptides provided a crucial test for the spectral interpretation via the mass shifts thus induced.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D007705:Kinins / Q000032:analysis*; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis*; D010803:Physalaemin / Q000032:analysis*; D011817:Rabbits; D012984:Software
D000596:Amino Acids; D007705:Kinins; D010455:Peptides; D010803:Physalaemin