Simultaneous demonstration of 3 synaptic markers: nerve endings, localization of AChE and AChR clusters at neuromuscular junctions of the rat in vitro.
Our triple labelling method allows the AChE and AChR distributions and the nerve endings to be stained in the same preparation. This method which provides a detailed visualization of the synaptic contacts and is capable of revealing a weak AChE activity is particularly valuable in studies of synaptogenesis.
D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D009411:Nerve Endings / Q000201:enzymology / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000196:embryology / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D011950:Receptors, Cholinergic / Q000032:analysis*; D013569:Synapses / Q000201:enzymology / Q000378:metabolism*
D011950:Receptors, Cholinergic; D000110:Acetylcholinesterase