Monoclonal antibodies for detection of lymphocyte markers: comparison of three commercial kits.
Three commercial monoclonal antibody kits (Histoset, OrthoDiagnostics, Raritan, NJ; T Cell Panel, Becton Dickinson, Mountain View, CA; Histotag Lymphoma Set, Hybritech Inc., San Diego, CA) were compared, using touch preparations, frozen sections, and formalin-fixed paraffin-embedded sections of 14 tonsils. With the first two kits, sensitive results were obtained in touch preparations and frozen sections with strong, crisp, reproducible immunostain. Results were accurate and easily interpreted despite moderate background staining. Distribution and numbers of lymphocytes and subsets were appropriate. The Histotag Lymphoma Set was sensitive for detection of T-cells in touch preparations and frozen sections. Hazy, moderate immunostain was obtained with B-cell and common leukocyte antibodies. Accuracy was questioned because of variable and false-negative results (75% in touch preparations) with all anti-sera, and interfollicular staining for B-lymphocytes. In formalin-fixed sections, positive results of low sensitivity were obtained only with the T Cell Panel. The Histoset and T Cell Panel are recommended, particularly for use with frozen sections.
D000911:Antibodies, Monoclonal / Q000032:analysis; D005188:False Negative Reactions; D005189:False Positive Reactions; D006651:Histocytochemistry; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000032:analysis*; D014066:Palatine Tonsil / Q000473:pathology; D011933:Reagent Kits, Diagnostic; D006377:T-Lymphocytes, Helper-Inducer / Q000032:analysis; D050378:T-Lymphocytes, Regulatory / Q000032:analysis
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic