[Relations between concanavalin A binding and cell proliferation. Comparison of Lyt-1+ and Lyt-2+ lymphocyte subpopulations from the spleen of unstimulated mice and mixed lymphocyte culture].
Fluorescein isothiocyanate conjugated concanavalin A (FITC-Con A) binding to negatively selected Lyt-1+ and Lyt-2+ spleen cells from nonstimulated mice and from mixed lymphocyte culture (MLC) was tested in parallel with the effect of Con A on proliferation of these cell subpopulations. Lyt-2+ spleen cells from nonstimulated mice bound more FITC-Con A but proliferated in response to Con A to lesser extent than Lyt-1+ cells. allogeneically stimulated Lyt-1+ cells were inhibited by Con A and bound FITC-Con A in higher amounts and more specifically than Lyt-2+ cells. In syngeneic control culture, no remarkable differences in both Con A binding and Con A-induced proliferation of the lymphocyte subpopulations were found. The results are discussed in context of Lyt-phenotypic and functional differences between T cell subpopulations and seem to contradict a strict correlation between Con A binding and cell function.
D000818:Animals; D002455:Cell Division; D003208:Concanavalin A / Q000378:metabolism*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008815:Mice, Inbred Strains; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D011952:Receptors, Concanavalin A; D003208:Concanavalin A
Untersuchungen zur Beziehung zwischen Concanavalin A-Bindung und Zellproliferation. Vergleich von Lyt-1+ und Lyt-2+ Lymphozytensubpopulationen aus Milzen von unstimulierten Mäusen und der gemischten Lymphozytenkultur.