Factors affecting solubilization of the D1 receptor from bovine caudate nucleus.
The D1 dopamine receptor was identified using the dopamine agonist 3H-ADTN. The ability of various detergents to solubilize this receptor from bovine caudate nucleus was examined. In order to maintain high affinity binding, receptors were solubilized in the presence of dopamine and manganese. GPP(NH)P reduced the yield of soluble receptors. Of the detergents tested, n-octyl-B-D-glucopyranoside gave the best yield of receptors capable of binding 3H-ADTN and related compounds with high affinity.
D000818:Animals; D002417:Cattle; D002421:Caudate Nucleus / Q000378:metabolism*; D004298:Dopamine / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D008566:Membranes / Q000378:metabolism; D011954:Receptors, Dopamine / Q000302:isolation & purification*; D017447:Receptors, Dopamine D1; D012995:Solubility; D013764:Tetrahydronaphthalenes / Q000494:pharmacology
D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D013764:Tetrahydronaphthalenes; D006165:Guanylyl Imidodiphosphate; D008345:Manganese; C011639:ADTN; D004298:Dopamine