Mast cell differentiation in cultures of T cell-depleted mesenteric lymph node cells from Nippostrongylus brasiliensis-infected mice.
Lymphoid and bone marrow cells from normal and horse serum-immunized mice and lymphoid cells from Nippostrongylus brasiliensis-infected mice were cultured on monolayers of embryonic skin fibroblasts to analyze the factors which regulate the differentiation and proliferation of mast cells in vitro. Our results indicate that T cells can regulate the development of mast cells in vitro by either enhancement or suppression. In cultures of horse serum-immune spleen cells, inducer T cells are required for mast cells to develop. However, in cultures of mesenteric lymph node cells from N. brasiliensis-infected mice, inducer T cells are not required for mast cell development. This suggests that the development of mast cells may occur at discrete interleukin-3 (IL-3)-dependent and IL-3-independent stages. Mast cell precursors in the mesenteric lymph nodes of N. brasiliensis-infected mice may have already been acted on by inducer cells in vivo to become mast cell committed. While IL-3 does not appear to be required for mast cell development, these precursors do require the presence of a connective tissue microenvironment such as embryonic skin. The precursors can be inhibited from development by interferon preparations.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology; D008212:Lymphocyte Depletion; D008407:Mast Cells / Q000166:cytology*; D008643:Mesentery; D051379:Mice; D008807:Mice, Inbred BALB C; D009349:Nematode Infections / Q000276:immunology / Q000473:pathology*; D009559:Nippostrongylus; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology
D007371:Interferon-gamma