Shedding of the common acute lymphoblastic leukemia antigen (CALLA) by lymphoblastoid cell lines.
The release of common acute lymphoblastic leukemia antigen (CALLA) into culture medium by lymphoblastoid cell lines was studied. CALLA release was detected using a solid phase indirect radioimmunometric assay which combines the specificity of lectins and monoclonal antibodies and using immunoadsorption of labeled CALLA in spent medium from cells incubated with 35S-methionine. No CALLA was present in the medium of cells pulse-labeled at 37 degrees C, when they were placed at 4 degrees C, thus this is an active process. Sequential analysis of soluble CALLA revealed shedding of CALLA to be associated with cell growth.
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000378:metabolism*; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D003470:Culture Media; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D015260:Neprilysin; D011863:Radioimmunoassay; D012995:Solubility; D013696:Temperature; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D003470:Culture Media; D006023:Glycoproteins; D008565:Membrane Proteins; D015260:Neprilysin