In-vitro biosynthesis of C18 neutral steroids in horse testes.
Deuterium, 14C- and 3H-labelled steroid substrates were incubated with minced testicular tissue from stallions of different ages. After extraction and separation of the neutral and phenolic fractions the metabolites were identified by gas chromatography-mass spectrometry. The presence of the expected C19 neutral and C18 phenolic steroids was confirmed. An isomer of 5(10)-oestrene-3,17-diol was also identified.
D000734:Androstenediols / Q000378:metabolism; D000735:Androstenedione / Q000031:analogs & derivatives / Q000378:metabolism; D000736:Androstenes / Q000378:metabolism*; D000818:Animals; D003687:Dehydroepiandrosterone / Q000096:biosynthesis; D004963:Estrenes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009277:Nandrolone / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis
D000734:Androstenediols; D000736:Androstenes; D004963:Estrenes; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D009277:Nandrolone; C002828:19-norandrostenedione