Effects of lipid fatty acyl chain structure on the activity of the (Ca2+ + Mg2+)-ATPase.
The (Ca2+ + Mg2+)-ATPase purified from rabbit muscle sarcoplasmic reticulum has been reconstituted into a series of phosphatidylcholines in the liquid crystalline phase. For phosphatidylcholines containing monounsaturated fatty acyl chains, optimal activity is observed for a chain length of C18, with longer or shorter chains supporting lower activities. Phospholipids with methyl-branched chain saturated fatty acids support somewhat lower activities than the corresponding phospholipids with mono-unsaturated fatty acids. Mixed chain phospholipids support ATPase activities comparable to those shown by an unmixed chain phospholipid with the same average chain length. However, the response of the ATPase reconstituted with mixed chain phospholipids to the addition of oleyl alcohol is dominated by the longest fatty acyl chain. Based on their ability to displace brominated phospholipids, relative binding constants to the ATPase of a series of phosphatidylcholines have been determined. Binding to the ATPase is virtually unaffected by fatty acyl chain length or the presence of methyl branches.
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology*; D005453:Fluorescence; D008051:Lipid Bilayers / Q000378:metabolism; D008560:Membrane Fluidity; D008745:Methylation; D009132:Muscles / Q000201:enzymology; D010713:Phosphatidylcholines / Q000378:metabolism / Q000494:pharmacology*; D011817:Rabbits; D013329:Structure-Activity Relationship; D013696:Temperature
D005227:Fatty Acids; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; C049875:1,2-di(9,10-dibromostearoyl)phosphatidylcholine; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases