von Willebrand factor contaminating porcine factor VIII concentrate (Hyate:C) causes platelet aggregation.
Severe thrombocytopenia has been reported to occur in some patients treated with a polyelectrolyte-fractionated porcine factor VIII concentrate (Hyate:C) (Kernoff et al, 1984; Gatti & Mannucci, 1984). We report here that Hyate:C induces aggregation of platelet-rich plasma in vitro accompanied by ATP release and thromboxane B2 formation, and is inhibited by EDTA and prostaglandin E1. Hyate:C induced platelet aggregation is affected by monoclonal antibodies anti-glycoprotein Ib or anti-glycoprotein IIb/IIIa and suppressed by polyclonal anti-porcine von Willebrand factor antiserum. We conclude that the aggregating ability of Hyate:C is due to porcine von Willebrand factor present in the concentrate. The occasional thrombocytopenia described after Hyate:C infusions probably results from the interaction between platelets and the porcine von Willebrand factor contaminating the concentrate.
D000255:Adenosine Triphosphate / Q000097:blood; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000378:metabolism; D004340:Drug Contamination; D004492:Edetic Acid / Q000494:pharmacology; D005169:Factor VIII / Q000494:pharmacology*; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology; D010974:Platelet Aggregation / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins; D013552:Swine; D013929:Thromboxane B2 / Q000097:blood; D014841:von Willebrand Factor / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins; D014841:von Willebrand Factor; D013929:Thromboxane B2; D000255:Adenosine Triphosphate; D005169:Factor VIII; D004492:Edetic Acid; D000527:Alprostadil