Molecular genetic analysis of the pyr-4 gene of Neurospora crassa.
By means of S1 nuclease mapping, one transcription origin and three termini are identified for the pyr-4 gene of Neurospora crassa, the same origin being used also by Escherichia coli on the cloned gene. Translation of the clone in mini-cells gives a 50,000 dalton gene product, the same size as that determined for the Neurospora native enzyme. Putative CAAT and TATA boxes, and upstream and downstream potential secondary structures, are identified.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D009690:Nucleic Acid Conformation; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D014158:Transcription, Genetic
D002262:Carboxy-Lyases; D009964:Orotidine-5'-Phosphate Decarboxylase