title: Determination of 5 alpha-androstane-3 alpha,17 beta-diol and 5 alpha-androstane-3 beta,17 beta-diol in human plasma by selected ion monitoring.
abstract: 5 alpha-Androstane-3 alpha,17 beta-diol (3 alpha-diol) and 5 alpha-androstane-3 beta,17 beta-diol (3 beta-diol) were measured in human peripheral plasma by gas chromatography-selected ion monitoring as their t-butyl dimethylsilyl ethers. The purification of the plasma extract involved a Sep-Pak C18 cartridge and high-performance liquid chromatography. The concentrations (mean +/- SD) of the two diols in four normal males are: 213 +/- 111 pg/ml for the 3 alpha-diol and 246 +/- 133 pg/ml for the 3 beta-diol.
mesh_terms: D015113:Androstane-3,17-diol / Q000097:blood*; D000732:Androstanols / Q000097:blood*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013237:Stereoisomerism
chemical_list: D000732:Androstanols; D015113:Androstane-3,17-diol