A mouse T cell product that preferentially enhances IgA production. I. Biologic characterization.
Supernatants from a subset of helper T cell clones can enhance IgA, IgE, and IgG1 production in cultures of lipopolysaccharide-stimulated, T cell-depleted spleen cells. The lymphokine interleukin (IL)-4 has been shown to cause the IgE and IgG1 enhancement produced by these supernatants. IgA enhancement, however, is mediated by a factor distinct from IL-4, although IL-4 can potentiate the effect of the IgA-enhancing factor. IgA-enhancing factor is also distinct from IL-1, IL-2, IL-3, granulocyte-macrophage colony-stimulating factor, and interferon-gamma and acts directly on B cells. Purified IgA-enhancing factor enhances IgA production three- to sixfold yet causes less than a twofold increase in other isotypes. The IgA enhancing activity is not inhibited by concentrations of interferon-gamma that inhibit IL-4 activities. In the accompanying article, we show that this IgA enhancing activity is a novel property of the lymphokine IL-5.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology; D015848:Interleukin-5; D007378:Interleukins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D008222:Lymphokines / Q000145:classification / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D013268:Stimulation, Chemical; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D007070:Immunoglobulin A; D015848:Interleukin-5; D007378:Interleukins; D008222:Lymphokines; D007371:Interferon-gamma