Pertussis toxin blocks autoreceptor-mediated inhibition of dopaminergic neurons in rat substantia nigra.
Rat substantia nigra was injected with 1 microgram of purified pertussis toxin, a substance which inactivates the inhibitory guanine nucleotide regulatory unit of adenylate cyclase. In comparison to saline-injected animals, the pertussis toxin-treated animals showed almost no inhibition of dopaminergic neurons in response to dopamine applied iontophoretically or the dopamine agonist (-)-apomorphine given intravenously. These results provide evidence that a guanine nucleotide regulatory protein is involved as a transducer in mediating the physiological actions of dopamine autoreceptors.
D000200:Action Potentials / Q000187:drug effects; D037361:Adenylate Cyclase Toxin; D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000502:physiology; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D009433:Neural Inhibition / Q000187:drug effects*; D037342:Pertussis Toxin; D051381:Rats; D011954:Receptors, Dopamine / Q000187:drug effects* / Q000502:physiology; D017448:Receptors, Dopamine D2; D013378:Substantia Nigra / Q000187:drug effects* / Q000502:physiology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D010566:Virulence Factors, Bordetella; D005680:gamma-Aminobutyric Acid; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D006220:Haloperidol; D001058:Apomorphine; D004298:Dopamine