Nature of atrial natriuretic polypeptide in rat brain.
Using reverse phase high performance liquid chromatography (RP-HPLC) coupled with two radioimmunoassays for atrial natriuretic polypeptide (ANP) with different specificities, we investigated the nature of alpha-rat ANP-like immunoreactivity (alpha-rANP-LI) with a low molecular weight in the rat brain. Two major peaks with alpha-rANP-LI in the extract from rat whole brains were eluted in the vicinity of the elution position of synthetic alpha-rANP, a 28-amino acid polypeptide. These two components co-migrated with synthetic alpha-rANP (4-28) and alpha-rANP (5-28), respectively. The peak corresponding to alpha-rANP (4-28) was the highest, and only a little alpha-rANP-LI was detected at the elution position of alpha-rANP. An identical profile in RP-HPLC was also observed in the extract from the rat hypothalamus. These results indicate that the major components of alpha-rANP-LI with a low molecular weight in the rat brain are alpha-rANP (4-28) and alpha-rANP (5-28).
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000302:isolation & purification*; D001923:Brain Chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008970:Molecular Weight; D011863:Radioimmunoassay; D051381:Rats
D009320:Atrial Natriuretic Factor