Mannose 6-phosphate/insulin-like growth factor II receptor: distinct binding sites for mannose 6-phosphate and insulin-like growth factor II.
Pentamannosyl phosphate substituted bovine serum albumin (PMP-BSA) and insulin like growth factor II (IGF II) bind specifically to immobilized mannose 6-phosphate/insulin like growth factor II receptor. An excess of IGF II inhibited binding of PMP-BSA by less than or equal to 20%, and an excess of PMP-BSA inhibited binding of IGF II by less than or equal to 10%. Polyclonal antibodies against the receptor purified from human liver inhibited preferentially the binding of PMP-BSA, and a monocloncal antibody 2C2 inhibited only the binding of IGF II to the receptor. Similar results were obtained for binding of PMP-BSA and IGF II to human skin fibroblasts. These results suggest that the binding sites for mannose 6-phosphate and IGF II reside in different portions of the receptor.
D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006600:Hexosephosphates / Q000378:metabolism*; D006801:Humans; D007158:Immunologic Techniques; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008099:Liver / Q000032:analysis; D008360:Mannosephosphates / Q000378:metabolism*; D017527:Receptor, IGF Type 2; D011972:Receptor, Insulin / Q000378:metabolism*; D017451:Receptors, Somatomedin; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013002:Somatomedins / Q000378:metabolism*
D002352:Carrier Proteins; D006600:Hexosephosphates; D007457:Iodine Radioisotopes; D008360:Mannosephosphates; D017527:Receptor, IGF Type 2; D017451:Receptors, Somatomedin; D013002:Somatomedins; C054881:pentamannosyl phosphate substituted bovine serum albumin; D012710:Serum Albumin, Bovine; C027693:mannose-6-phosphate; D007335:Insulin-Like Growth Factor II; D011972:Receptor, Insulin