Genetic and biochemical characterization of phosphofructokinase from Drosophila melanogaster.
Phosphofructokinase (PFK;EC 2.7.1.11) activity in Drosophila melanogaster is controlled by a single dosage-sensitive region of the genome between 45F and 47E of chromosome IIR. Only a single form of PFK was detected electrophoretically in both adults and larvae. Nearly 90% of the PFK activity in adults is localized to the thorax. Purification of the enzyme was hampered by the extreme lability of Drosophila PFK; however, a 36-fold partial purification was achieved.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000782:Aneuploidy; D000818:Animals; D004303:Dosage Compensation, Genetic; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D007814:Larva; D008970:Molecular Weight; D010732:Phosphofructokinase-1 / Q000235:genetics / Q000302:isolation & purification*; D011679:Pupa
D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1