title: Comparative analysis of in vitro fertilization methods for establishing successful embryo transfer clinics.
abstract: A review of the laboratory methods and embryo transfer procedures used by seven of the foremost clinics in the world are discussed and evaluated in this article. Based on these facts an in vitro fertilization clinic was launched in 1983. In the second part of the paper the latest laboratory methods, i.e. ovulation induction plans and the embryo transfer technique of Tygerberg Hospital, are described. One hundred and thirty laparoscopies were performed since 1 May 1984-30 November 1984. Six hundred and one follicles were aspirated, 482 oocytes were obtained, 84% of all oocytes were fertilized, 112 patients reached the transfer stage and 28 pregnancies resulted. A clinical pregnancy rate of 21,4% per transfer and 18,5% per laparoscopy resulted.
mesh_terms: D001769:Blood; D002021:Buffers; D002245:Carbon Dioxide; D002404:Catheterization / Q000295:instrumentation; D003470:Culture Media; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D004624:Embryo Transfer / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010535:Laparoscopy; D008297:Male; D009584:Nitrogen; D009865:Oocytes / Q000502:physiology; D009994:Osmolar Concentration; D010062:Ovulation Induction; D010100:Oxygen; D011182:Postoperative Care; D011187:Posture; D011247:Pregnancy; D011292:Premedication; D012661:Semen / Q000502:physiology; D013076:Sperm Count; D013094:Spermatozoa / Q000502:physiology; D014599:Uterus / Q000033:anatomy & histology; D014867:Water / Q000592:standards
chemical_list: D002021:Buffers; D003470:Culture Media; D014867:Water; D002245:Carbon Dioxide; D009584:Nitrogen; D010100:Oxygen