title: Structure and formation of microplasmin.
abstract: The structure of human microplasmin, prepared from plasmin in alkaline solution, has been studied. Microplasmin consists of two polypeptide chains connected by disulfide bonds. One polypeptide is the B chain of plasmin consisting of 230 amino acids, and the other peptide is the COOH-terminal portion of the A chain of plasmin consisting of 31 amino acid residues. Microplasmin has a molecular weight of 28,635, calculated from its primary sequence. It is slightly more positively charged than plasminogen and is a more hydrophobic molecule. The proposed scheme for the formation of microplasmin involves autolysis at specific peptide bonds and scrambling of especially sensitive disulfide bonds in alkaline solution.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides; D005341:Fibrinolysin / Q000096:biosynthesis; D006801:Humans; D006868:Hydrolysis; D010446:Peptide Fragments / Q000096:biosynthesis; D011487:Protein Conformation
chemical_list: D000596:Amino Acids; D004220:Disulfides; D010446:Peptide Fragments; C054561:microplasmin; D005341:Fibrinolysin