title: [Measuring blood flow in microsurgically revascularized, immunosuppressed segmental allogeneic bone transplants using laser Doppler flowmetry].
abstract: This paper presents Laser Doppler Flowmetry (LDF) as a method for measuring cortical bone blood flow. The goal of this study was to evaluate the efficacy of LDF as a means to determine cortical bone perfusion as an indicator of persistent patency of microvascular anastomoses. Cortical bone blood flow was measured in an experiment using free vascularized immunosuppressed bone allografts. Disulfine blue demonstrated patency in all grafts at 20 weeks sacrifice date, with 15 percent localized areas of nonperfusion, corresponding to areas of microscopic evidence of devitalization. LDF suggested four out of six anastomoses to be nonpatent at four weeks, and irregular evidence of patency at 20 weeks, depending the site of measurement. The small volume of tissue sampled by the probe limits LDF's ability to assess patency of a vessel supplying a whole graft. Comparing LDF with disulfine blue technique, we conclude that the LDF-results in our experiment did not allow general statements regarding the patency of microvascular anastomoses to large segments of cortical bone.
mesh_terms: D000818:Animals; D001158:Arteries / Q000601:surgery; D016025:Bone Transplantation; D001842:Bone and Bones / Q000098:blood supply; D003524:Cyclosporins / Q000627:therapeutic use; D004285:Dogs; D005260:Female; D007834:Lasers; D008866:Microsurgery; D012039:Regional Blood Flow / Q000187:drug effects; D012212:Rheology
chemical_list: D003524:Cyclosporins
vernacular_title: Durchblutungsmessung bei mikrochirurgisch revaskularisierten immunsupprimierten segmentalen allogenen Knochentransplantaten mittels "Laser Doppler Flowmetry".