T cell receptor-homologous mRNA from a suppressor T cell clone directs the synthesis of antigen-specific suppressive products.
The LH8-105 T cell clone, obtained by radiation leukemia virus-induced transformation of hen egg-white lysozyme (HEL)-specific mouse suppressor T lymphocytes, constitutively releases in the culture supernatant products able to induce specific suppression of the immune response to HEL. LH8-105 cells rearrange and express the genes encoding the alpha and beta chains of the antigen-specific T cell receptor and display membrane T cell receptor structures. LH8-105 mRNA specific for the alpha and beta chains of the T cell receptor were positively selected on filter-bound cDNA encoding constant regions of alpha and beta chains, eluted and translated into Xenopus laevis oocytes. Translation products were then tested in vivo for specific suppression of the anti-HEL antibody response. LH8-105 culture supernatant and translation products of LH8-105 poly(A)+ RNA or a mixture of alpha and beta chain-homologous mRNA induce HEL-specific suppression whereas translation products of LH8-105 mRNA eluted from irrelevant DNA or unrecombined mRNA eluted from alpha and beta cDNA do not exert suppressive activity. These results indicate that alpha and beta chain-homologous mRNA expressed in LH8-105 cells direct the synthesis of molecules able to induce HEL-specific suppression.
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D007108:Immune Tolerance; D051379:Mice; D009113:Muramidase / Q000276:immunology; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013491:Suppressor Factors, Immunologic / Q000235:genetics*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D010446:Peptide Fragments; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013491:Suppressor Factors, Immunologic; D009113:Muramidase