Partial purification and characterization of dynein adenosine triphosphatase from bovine sperm.
Outer dynein arm polypeptides that possess Mg+2-adenosine triphosphatase (ATPase) activity have been extracted from the flagellar axonemes of demembranated bovine sperm. Electron microscopy of intact and salt-extracted sperm demonstrates a relatively selective removal of the outer dynein arms. The salt extract contains a specific ATPase activity of 55 nmoles inorganic phosphate (Pi)/min/mg protein. Sucrose density gradient centrifugation of this extract results in a 6-fold increase in specific activity of ATPase (333 nmole/Pi/min/mg protein), which sediments as a single 13S peak. Concomitant with the increase in specific activity, there is enrichment of three high molecular weight polypeptides (Mr greater than 300,000) characteristic of dynein heavy chains. ATPase activities in the initial extract and in the 13S peak are inhibited by concentrations of vanadate and erythro-9-[3-2-(hydroxynonyl)]adenine similar to those that inhibit ATPase activity in sea urchin sperm dynein. These findings indicate that outer arm dynein ATPase can be extracted and partially purified from bovine sperm.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification*; D000818:Animals; D001386:Azides / Q000494:pharmacology; D002417:Cattle; D002499:Centrifugation, Density Gradient; D004398:Dyneins / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D010042:Ouabain / Q000494:pharmacology; D010449:Peptide Mapping; D013082:Sperm Tail / Q000201:enzymology; D013094:Spermatozoa / Q000201:enzymology*; D014638:Vanadates / Q000494:pharmacology
D001386:Azides; D014638:Vanadates; D010042:Ouabain; D000251:Adenosine Triphosphatases; D004398:Dyneins