Cytotoxic T lymphocyte-mediated lysis via the Fc receptor of target cells.
The murine monoclonal antibody F23.1 reacts with an allotypic determinant on the T cell receptors of about 25% of peripheral T lymphocytes in most common mouse strains. In this report we demonstrate that this IgG2a antibody bound to the receptor of a cytotoxic effector T cell can direct that cell to lyse any target cell which expresses receptors for IgG2a. Thus, allotype-positive, Lyt-2+ T lymphocytes from either concanavalin A- or F23.1-activated cell cultures are recruited as cytotoxic effector cells which lyse targets via the Fc receptor. Concanavalin A-induced T cell blasts depleted of Lyt-2+ cells do not mediate cytotoxic effects in this system. The data presented here confirm earlier observations that monoclonal anti-T cell receptor antibodies attached to the surface of a cell can act as target structures for T cell-mediated lysis.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology*; D017452:Receptors, IgG; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D011948:Receptors, Antigen, T-Cell; D011961:Receptors, Fc; D017452:Receptors, IgG; D003208:Concanavalin A