A single receptor binds both insulin-like growth factor II and mannose-6-phosphate.
Amino acid sequences deduced from rat complementary DNA clones encoding the insulin-like growth factor II (IGF-II) receptor closely resemble those of the bovine cation-independent mannose-6-phosphate receptor (Man-6-P receptorCI), suggesting they are identical structures. It is also shown that IGF-II receptors are adsorbed by immobilized pentamannosyl-6-phosphate and are specifically eluted with Man-6-P. Furthermore, Man-6-P specifically increases by about two times the apparent affinity of the purified rat placental receptor for 125I-labeled IGF-II. These results indicate that the type II IGF receptor contains cooperative, high-affinity binding sites for both IGF-II and Man-6-P-containing proteins.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D002846:Chromatography, Affinity; D004247:DNA / Q000235:genetics; D005260:Female; D006600:Hexosephosphates / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D008360:Mannosephosphates / Q000378:metabolism*; D008969:Molecular Sequence Data; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D051381:Rats; D017527:Receptor, IGF Type 2; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism*; D017451:Receptors, Somatomedin; D012689:Sequence Homology, Nucleic Acid; D013002:Somatomedins / Q000378:metabolism*
D002352:Carrier Proteins; D006600:Hexosephosphates; D008360:Mannosephosphates; D017527:Receptor, IGF Type 2; D017451:Receptors, Somatomedin; D013002:Somatomedins; C027693:mannose-6-phosphate; D007335:Insulin-Like Growth Factor II; D004247:DNA; D011972:Receptor, Insulin