Laminin receptors for neurite formation.
Laminin, a basement membrane glycoprotein promotes both cell attachment and neurite outgrowth. Separate domains on laminin elicit these responses, suggesting that distinct receptors occur on the surface of cells. NG108-15 neuroblastoma-glioma cells rapidly extend long processes in the presence of laminin. We report here that 125I-labeled laminin specifically binds to these cells and to three membrane proteins of 67, 110, and 180 kDa. These proteins were isolated by affinity chromatography on laminin-Sepharose. The 67-kDa protein reacted with antibody to the previously characterized receptor for cell attachment to laminin. Antibodies to the 110-kDa and 180-kDa bands demonstrated that the 110-kDa protein was found in a variety of epithelial cell lines and in brain, whereas the 180-kDa protein was neural specific. Antibodies prepared against the 110-kDa and 180-kDa proteins inhibited neurite outgrowth induced by the neurite-promoting domain of laminin, whereas antibodies to the 67-kDa laminin receptor had no effect on neurite outgrowth. We conclude that neuronal cells have multiple cell-surface laminin receptors and that the 110-kDa and 180-kDa proteins are involved in neurite formation.
D000912:Antibodies, Neoplasm / Q000276:immunology; D002846:Chromatography, Affinity; D005910:Glioma / Q000473:pathology; D007797:Laminin / Q000378:metabolism / Q000494:pharmacology*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D011971:Receptors, Immunologic / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D017474:Receptors, Laminin; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure
D000912:Antibodies, Neoplasm; D007797:Laminin; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011971:Receptors, Immunologic; D017474:Receptors, Laminin