The immunoglobulin heavy chain enhancer is stimulated by the adenovirus type 2 E1A products in mouse fibroblasts.
In contrast with our previous results (Hen, R., Borrelli, E. & Chambon, P. (1985) Science 230, 1391-1394), which demonstrated that the mouse immunoglobulin heavy chain transcriptional enhancer is repressed in lymphoid cells by the products of the adenovirus type 2 E1A transcription unit, we show here that these products activate the same enhancer in mouse fibroblast L cell lines that contain stably integrated copies of a recombinant in which the enhancer is inserted upstream from the chicken conalbumin promoter. In addition, competition experiments suggest that the activity of the heavy chain enhancer may be repressed by a trans-acting factor in mouse L cells. We speculate that the E1A products may prevent the action of this cellular repressor in these cells.
D017456:Adenovirus Early Proteins; D000818:Animals; D004742:Enhancer Elements, Genetic; D005347:Fibroblasts / Q000378:metabolism; D005809:Genes, Regulator; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007739:L Cells / Q000378:metabolism; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000494:pharmacology*; D012097:Repressor Proteins / Q000502:physiology; D014158:Transcription, Genetic
D017456:Adenovirus Early Proteins; D007143:Immunoglobulin Heavy Chains; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins