Transcellular transport of lipoprotein through arterial endothelial cells in monolayer culture.
To study the mechanism of lipoprotein transport through arterial endothelial cells, porcine endothelial cells were cultured on gelated type I collagen supported by a dacron sheet, and the transport of low density lipoprotein (LDL) labeled with rhodamine B isothiocyanate (RB-LDL) through the cells was measured. Light and scanning electron microscopy showed that the cells on the gel were confluent. There was little RB-LDL transport through the endothelial monolayer at 0 degrees C. RB-LDL transport through the monolayer at 37 degrees C was dose-dependent saturable at 0.4 mg protein/ml. The transport was energy-dependent, since its rate was affected by temperature and was inhibited by a combination of 2-deoxyglucose (50 mM) and NaN3 (10 mM). RB-LDL was shown not to be degraded during transport.
D000818:Animals; D001158:Arteries / Q000166:cytology*; D001345:Autoradiography; D001692:Biological Transport; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000378:metabolism* / Q000648:ultrastructure; D004735:Energy Transfer / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011093:Polyethylene Terephthalates; D012235:Rhodamines; D013552:Swine; D013696:Temperature
D003470:Culture Media; D008077:Lipoproteins, LDL; D011093:Polyethylene Terephthalates; D012235:Rhodamines; D003094:Collagen; C027755:rhodamine isothiocyanate