A comparative marker study of large cell lymphoma, Hodgkin's disease, and true histiocytic lymphoma in paraffin-embedded tissue.
A comparative study of large cell lymphoma (LCL) (ten B and ten T), Hodgkin's disease (15 cases), and true histiocytic lymphoma (two cases) was undertaken, using formalin-fixed paraffin-embedded tissue sections, a panel of eight antibodies, and one lectin to determine if any particular antibody or immunologic profile could reliably distinguish between these entities. The antibodies used were against Leu-M1, alpha-1-anti-chymotrypsin (alpha-ACT), alpha-anti-trypsin (alpha-AT), lysozyme, kappa, lambda, leukocyte common antigen (LCA), and S-100 protein. The lectin used was peanut agglutinin (PNA). Although Leu-M1 staining was positive in 11 of 15 cases (73%) of Hodgkin's disease, it was also positive in 4 of 10 cases (40%) of T-cell lymphoma, 2 of 10 cases (20%) of B-cell lymphoma, and 1 of 2 cases (50%) of true histiocytic lymphoma. Peanut-agglutinin staining results were similar to Leu-M1. The only staining profile that emerged was the presence of Leu-M1, PNA-, alpha-ACT, and alpha-AT staining in Reed-Sternberg (RS) cells in 11 of 15 cases of Hodgkin's disease. Leu-M1 and its staining pattern is characteristic, but not entirely specific for RS cells, and it was not positive in at least 25% of the cases of Hodgkin's disease in formalin-fixed, paraffin-embedded tissues. The limitations of this antibody and others should be recognized.
D000951:Antigens, Neoplasm / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology; D006689:Hodgkin Disease / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000276:immunology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D017493:Leukocyte Common Antigens; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology*; D009113:Muramidase / Q000276:immunology; D019887:Peanut Agglutinin; D009418:S100 Proteins / Q000276:immunology; D000514:alpha 1-Antichymotrypsin / Q000276:immunology; D000515:alpha 1-Antitrypsin / Q000276:immunology
D000951:Antigens, Neoplasm; D006649:Histocompatibility Antigens; D037102:Lectins; D019887:Peanut Agglutinin; D009418:S100 Proteins; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D017493:Leukocyte Common Antigens; D009113:Muramidase