Mapping and regulation of the cel genes in Erwinia chrysanthemi.
Chromosomal mutations of the celZ and celY genes which encode two different endoglucanases in Erwinia chrysanthemi 3937 were obtained by a three-step procedure: (i) in Escherichia coli, insertions of lacZ fusion-forming mini-Mu bacteriophages in the cel genes cloned on plasmids and screening of cel-lac fusions, (ii) Mu-mediated transduction in E. chrysanthemi of the plasmids carrying the fusions, (iii) recombinational exchange between the plasmidic mutated and the wild-type chromosomal alleles. These mutations allowed mapping of celZ between ura and pan and celY between xyl and met on the linkage map of E. chrysanthemi. The beta-galactosidase activity of these strains indicated that celZ is expressed in the late exponential and stationary growth phases, while celY expression is almost undetectable.
D010583:Bacteriophage mu / Q000235:genetics; D002480:Cellulase / Q000235:genetics*; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis; D004885:Erwinia / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial
D002480:Cellulase