Interleukin induction of non-specific suppressor cells.
We analysed the cellular and molecular signals involved in the induction of non-specific T suppressor (Ts) cells generated in mouse spleen cell suspensions after 3-4 days' culture in the absence of conventional antigen. Using an in vitro model of primary antibody response as a suppressor assay, we showed that purified T cells were unable to generate suppressive activity. This suppression was fully restored when T cells were cultured with macrophages. In this case, Ts-cell generation was markedly increased by the addition of indomethacin suggesting a negative effect of prostaglandins in this phenomenon. Macrophages could be replaced by semi-purified IL1 as well as by recombinant IL2. Thus, in conditions of severe macrophage depletion, IL1 and/or IL2 are fully capable of inducing Ts-cell generation.
D000818:Animals; D000917:Antibody Formation; D002478:Cells, Cultured; D006801:Humans; D007165:Immunosuppression Therapy; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D007375:Interleukin-1; D007376:Interleukin-2; D007213:Indomethacin