Rhamnolipid from Pseudomonas aeruginosa inactivates mammalian tracheal ciliary axonemes.
Isolated ciliary axonemes from pig trachea were exposed to increasing concentrations of purified Pseudomonas aeruginosa rhamnolipid. This is a defined ciliary system allowing observation of direct impairment of functional axonemes. Axonemal motility and ATPase activity were decreased in proportion to rhamnolipid concentrations. ATPase-associated proteins observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dynein arms seen in ultra-structural cross sections progressively disappeared from axonemes with exposure to rhamnolipid. These four independent measures establish that the rhamnolipid removes the ATPase-containing outer dynein arms from the ciliary axoneme, thereby rendering the axoneme immotile.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D002923:Cilia / Q000187:drug effects / Q000201:enzymology; D006017:Glycolipids / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013552:Swine; D014132:Trachea / Q000187:drug effects* / Q000201:enzymology
D006017:Glycolipids; D000251:Adenosine Triphosphatases