A novel stimulator of protein phosphorylation in Neurospora crassa.
An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100,000 X g supernatants of Neurospora crassa mycelial extracts. This 38,000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed.
D002850:Chromatography, Gel; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology* / Q000254:growth & development; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*
D005656:Fungal Proteins; D011494:Protein Kinases