Sequential detection of antigens in Western blots with differently colored products.
A reliable sequential application of peroxidase and alkaline phosphatase-conjugated second antibodies to detection of two different antigens in Western blots is described. Deposits of differently colored products formed allow identification of several antigens on a single blot.
D000469:Alkaline Phosphatase; D000941:Antigens / Q000302:isolation & purification*; D002966:Clathrin / Q000276:immunology / Q000302:isolation & purification; D003101:Collodion; D003116:Color; D004591:Electrophoresis, Polyacrylamide Gel; D006596:Hexosaminidases / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification; D010544:Peroxidases; D011487:Protein Conformation; D001619:beta-N-Acetylhexosaminidases
D000941:Antigens; D002966:Clathrin; D007527:Isoenzymes; D003101:Collodion; D010544:Peroxidases; D000469:Alkaline Phosphatase; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases