Membrane glycoproteins are involved in the differentiation of the BC3H1 muscle cell line.
The nonfusing muscle cell line BC3H1 expresses a family of muscle-specific proteins when the fetal bovine serum (FBS) concentration is reduced from 20 to 1%. We have used a series of glycosylation inhibitors to assess the role played by glycoproteins in the initiation of differentiation in this cell line. Tunicamycin (TNM) and 2-deoxy-D-glucose, added to cells when the FBS concentration was reduced, blocked creatine phosphokinase (CPK) induction by 70-95%. These effects were dose dependent and reversible. TNM and 2-deoxy-D-glucose also reversed CPK induction in differentiated cells. Leupeptin and N-acetylglucosamine did not reverse these effects. 1-Deoxynojirimycin, 1-deoxymannojirimycin, and swainsonine have no effect on induced CPK expression, whereas castanospermine, a glucosidase I inhibitor, blocked its induction completely. As attempts to use conditioned medium from cells grown in 1 or 20% FBS have no effect on this differentiation process we conclude that high mannose structures, but not complex form glycoproteins, bound to the surface of BC3H1 cells play a role in transducing signals for differentiation and are probable mediators of cell/cell contact.
D017485:1-Deoxynojirimycin; D000117:Acetylglucosamine / Q000494:pharmacology; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003402:Creatine Kinase / Q000096:biosynthesis; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D004790:Enzyme Induction; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism* / Q000502:physiology; D007212:Indolizines; D007976:Leupeptins / Q000494:pharmacology; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D017026:Swainsonine; D014415:Tunicamycin / Q000494:pharmacology; D054836:Indolizidines
D017485:1-Deoxynojirimycin; D000117:Acetylglucosamine; D000470:Alkaloids; D003402:Creatine Kinase; D003837:Deoxy Sugars; D003847:Deoxyglucose; D005944:Glucosamine; D006023:Glycoproteins; D007212:Indolizines; D007976:Leupeptins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009844:Oligosaccharides; D017026:Swainsonine; D014415:Tunicamycin; C025363:mannoproteins; C032854:leupeptin; C037806:castanospermine; D054836:Indolizidines