Selective elimination of anti-DNA antibody-producing cells by antiidiotypic antibody conjugated with neocarzinostatin.
A new strategy was shown for the manipulation of autoantibody production in humans. Antiidiotypic antibody to human anti-DNA autoantibody was conjugated with neocarzinostatin (NCS), a cytotoxic agent, by using N-succimidyl 3-(2-pyridyldithio) propionate as a coupling agent. Human B cell clones, which produce anti-DNA autoantibodies, were killed by in vitro treatment with antiidiotype (Id)-NCS conjugates, while clones expressing an Id with irrelevant specificity were unaffected. These results indicate that treatment with anti-Id-NCS conjugates can act as a potent and specific means of generating immunosuppression of autoantibody production. This approach will have a significant advantage in aborting clones that are not effectively suppressed for the autoantibodies by anti-Id antibodies alone, and will result in a potential therapeutic treatment for systemic lupus erythematosus.
D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000302:isolation & purification*; D001402:B-Lymphocytes / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000276:immunology*; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D009353:Zinostatin / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007130:Immunoglobulin Idiotypes; D004247:DNA; D009353:Zinostatin