title: Analysis of responding lymphocyte subsets in allogeneic mixed lymphocyte cultures in the neonates and adults.
abstract: An analysis of proliferating cell subsets in the allogeneic mixed lymphocyte culture (MLC) in adults (n = 19) and newborns (n = 12) was performed by a method combining immunoperoxidase staining and autoradiography. The main cell type responding in adult cells were OKT8-positive (38%), whereas in neonates OKT4-positive cells (31%) predominated. Furthermore, in the neonatal allogeneic MLC there were significantly more 3H-thymidine incorporating Leu 12-positive B cells than in adults (12% vs 4%).
mesh_terms: D000328:Adult; D000954:Antigens, Surface / Q000032:analysis; D001345:Autoradiography; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000145:classification* / Q000276:immunology
chemical_list: D000954:Antigens, Surface