CD3 modulation inhibits pokeweed mitogen-induced T-cell help for immunoglobulin production.
The CD3 molecule is considered to be a signal transducer in the process of T-cell activation. Modulation of the CD3 molecule of peripheral blood T cells can be accomplished by incubation at 37 degrees C with UCHT-1, a mouse IgG1 anti-CD3 monoclonal antibody, under experimental conditions avoiding T-cell activation. We have examined the effect of CD3 modulation on T-cell-dependent polyclonal immunoglobulin (Ig) production induced by pokeweed mitogen (PWM) in cultures of peripheral blood lymphocytes. CD3 modulation strongly inhibited (greater than 80%) IgG and IgM production. This was due to inhibition of the production of soluble helper factors by the T cells, and not to induction of suppressor cells. These data support the concept that the CD3 molecule is an essential signal transducer in the process of PWM-induced helper T-cell activity, and that CD3 can function as a receptor transmitting negative signals to helper T cells.
D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D011043:Pokeweed Mitogens / Q000037:antagonists & inhibitors; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D011043:Pokeweed Mitogens