Molecular structure of the gene and the 5'-flanking region of the human lymphocyte immunoglobulin E receptor.
Overlapping clones which contain the complete gene encoding the human lymphocyte IgE receptor (MW:45kd; identical with CD23), were isolated from human genomic lambda-libraries. The gene spans approximately 13kb and comprises 11 exons. The 5'-end of the mRNA was mapped by primer extension and S1-mapping, revealing two initiation sites for transcription. Two corresponding TATA boxes were identified by sequencing the 5'-flanking region. A 188bp long inverted repeat was found which flanks the promoter region and could possibly be involved in gene regulation. Exons 9 to 11 code for the IgE-binding domain of the receptor which shows homology to several lectins, particularly to the asialoglycoprotein receptor. A comparison of the exon/intron arrangement of these genes implies that their lectin domains have evolved from a common ancestral cassette.
D001483:Base Sequence; D005260:Female; D005796:Genes; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011961:Receptors, Fc / Q000235:genetics*; D017455:Receptors, IgE; D011975:Receptors, Mitogen / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D011961:Receptors, Fc; D017455:Receptors, IgE; D011975:Receptors, Mitogen