title: Laser desorption/Fourier transform mass spectra of glycoalkaloids and steroid glycosides.
abstract: Positive- and negative-ion mass spectra of five glycoconjugates were obtained using laser desorption/Fourier transform mass spectrometry. These were the glycoalkaloids alpha-solanine and alpha-tomatine and the steroid glycosides gitoxin, lanatoside A and digitonin. Doping with KCl yielded both potassium- and chloride-attachment ions. Few fragment ions were observed for these species, with the exception of digitonin, although the negative-ion spectra showed relatively more fragmentation than the positive-ion spectra. All major fragments appeared to arise from losses of sugar groups due to cleavages at the glycosidic linkages. This contrasted sharply with the behavior of the malto-oligosaccharides studied in this laboratory.
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D004072:Digitonin / Q000032:analysis; D004077:Digoxin / Q000032:analysis / Q000031:analogs & derivatives; D005583:Fourier Analysis; D006027:Glycosides / Q000032:analysis*; D007799:Lanatosides / Q000032:analysis; D007834:Lasers; D013058:Mass Spectrometry; D012992:Solanine / Q000032:analysis; D013256:Steroids / Q000032:analysis*; D014053:Tomatine / Q000032:analysis
chemical_list: D000470:Alkaloids; D006027:Glycosides; D007799:Lanatosides; D013256:Steroids; D012992:Solanine; D014053:Tomatine; D004077:Digoxin; C036572:lanatoside A; D004072:Digitonin; C005003:gitoxin