Enhancement of murine thyroid allograft survival after 16 to 20 hours' organ culture.
Fully allogeneic thyroid organs cultured 16-20 hr in Hanks' balanced salt solution (HBSS) were transplanted to mice under the kidney capsule. Organ cultures maintained in 5% CO2 and air or in 100% nitrogen atmosphere in HBSS survived beyond the normal period required for graft rejection, in contrast to those maintained in room air or thyroids cultured in Eagle's minimum essential medium. Rejection of HBSS-cultured allogeneic thyroids can be induced by the injection of donor peritoneal exudate cells into the recipient 2 days after transplant. Lymphoid stimulator cells cultured overnight in HBSS, but not in (Eagle's) minimum essential medium in 5% CO2 and air, failed to induce alloreactive cytotoxic T cells in mixed lymphocyte cultures in vitro. The data suggest that short-term organ culture in a chemically simpler and nutritionally deficient medium in a low-oxygen environment enhances allograft survival. This is in contrast to the presently held theory, in which high-oxygen concentration is believed responsible for enhancement of allograft survival. This procedure should prove helpful in further determination of the factors involved in homograft rejection.
D000388:Air; D000818:Animals; D002021:Buffers / Q000494:pharmacology; D002245:Carbon Dioxide / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D006085:Graft Survival / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000637:transplantation; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000276:immunology; D008808:Mice, Inbred CBA / Q000276:immunology; D009584:Nitrogen / Q000494:pharmacology; D009924:Organ Culture Techniques / Q000379:methods*; D010100:Oxygen / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D013961:Thyroid Gland / Q000166:cytology / Q000637:transplantation*; D013997:Time Factors; D014184:Transplantation, Homologous
D002021:Buffers; D003470:Culture Media; D002245:Carbon Dioxide; D009584:Nitrogen; D010100:Oxygen