gp 140, the C3d/EBV receptor (CR2), is phosphorylated upon in vitro activation of human peripheral B lymphocytes.
gp 140, the C3d/EBV receptor (CR2), is a specific marker of human B lymphocytes. Very recent data suggest that CR2 is a membrane site involved in early B cell activation. These properties of CR2 led us to analyze the molecular events associated with gp 140. We analyzed whether in some conditions of B lymphocyte activation, CR2 could be phosphorylated. We have found that when highly enriched peripheral B cells were cultured for 48 h with anti-mu Ab and/or SAC, in order to provide an optimal activating signal, phosphorylation of the CR2 was induced.
D001402:B-Lymphocytes / Q000378:metabolism*; D002478:Cells, Cultured; D003176:Complement C3; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007163:Immunosorbent Techniques; D008213:Lymphocyte Activation; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011951:Receptors, Complement / Q000378:metabolism*; D017464:Receptors, Complement 3d; D011991:Receptors, Virus / Q000378:metabolism*
D003176:Complement C3; D010750:Phosphoproteins; D011951:Receptors, Complement; D017464:Receptors, Complement 3d; D011991:Receptors, Virus