Long-wavelength fluorescence of tyrosine and tryptophan solutions.
We report in this paper the presence of fluorescence bands of tryptophan and tyrosine solutions centered above 550 nm. This long-wavelength fluorescence is of much lower intensity, (0.4-2.7)%, than the UV fluorescence of these aromatic aminoacids. The basic characteristic of these fluorescence bands are: (a) tyrosine: lambda em = 600 nm with two excitation peaks centered at 453 nm and 550 nm (b) tryptophan: lambda em = 675 nm with two excitation peaks centered at 455 and 560 nm. It has been found that irradiation of tyrosine solutions with a potent UV lamp promotes an important increase of absorption at 310 nm and above 400 nm.
D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D012519:Sarcoplasmic Reticulum / Q000201:enzymology; D012996:Solutions / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014364:Tryptophan / Q000032:analysis*; D014443:Tyrosine / Q000032:analysis*
D012996:Solutions; D014443:Tyrosine; D014364:Tryptophan; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases