title: Simultaneous demonstration of 3 synaptic markers: nerve endings, localization of AChE and AChR clusters at neuromuscular junctions of the rat in vitro.
abstract: Our triple labelling method allows the AChE and AChR distributions and the nerve endings to be stained in the same preparation. This method which provides a detailed visualization of the synaptic contacts and is capable of revealing a weak AChE activity is particularly valuable in studies of synaptogenesis.
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D009411:Nerve Endings / Q000201:enzymology / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000196:embryology / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D011950:Receptors, Cholinergic / Q000032:analysis*; D013569:Synapses / Q000201:enzymology / Q000378:metabolism*
chemical_list: D011950:Receptors, Cholinergic; D000110:Acetylcholinesterase