HLA-DR expression on cytotoxic T lymphocytes.
A DR-null and DQ-null mutant lymphoblastoid cell line (LCL) was used to generate cytotoxic T cells (CTL) to investigate DR expression on CTL. Effector T cells generated in human allogeneic mixed lymphocyte culture (MLC) were separated on a fluorescence-activated cell sorter (FACS) into DR-positive and DR-negative subsets. The great majority of detectable cytolytic activity, anti-class I and anti-class II (presumably anti-DP) CTL, as well as natural killer (NK)-like activity, resided within the DR-positive population.
D000911:Antibodies, Monoclonal; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II