title: The 140-kDa fibronectin receptor complex is required for mesodermal cell adhesion during gastrulation in the amphibian Pleurodeles waltlii.
abstract: We have studied the localization and function of a 140-kDa glycoprotein complex implicated in cell adhesion to fibronectin- and laminin-rich extracellular matrices in Pleurodeles waltlii gastrulae. In particular, we have shown that antibodies directed against highly purified avian fibronectin (FN) receptor complex cross-react with two major polypeptides of apparent molecular weights of 140,000 and 100,000 and a third minor component of 90,000. Using sections of embryos or whole mounts, we have also discovered that the putative FN receptor is widely distributed on the early embryonic cell surface. We have also found that the basal surface of the roof of the blastocoel, a region particularly enriched in an extracellular matrix consisting of fibronectin- and laminin-rich fibrils, is rich in receptor complex. We have prepared monovalent Fab' fragments of this antibody and have found that they cause detachment of cells previously attached to substrata coated with fibronectin, and they also arrest gastrulation when injected into the blastocoel of early gastrulae. Thus, it appears that the fibronectin receptor complex plays a significant functional role in cell attachment of gastrula-stage cells in vitro and in cell migration in vivo during gastrulation.
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005775:Gastrula / Q000032:analysis / Q000166:cytology* / Q000648:ultrastructure; D007118:Immunoassay; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008648:Mesoderm / Q000166:cytology*; D008845:Microinjections; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000032:analysis; D010999:Pleurodeles / Q000196:embryology*; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D012455:Salamandridae / Q000196:embryology*
chemical_list: D007140:Immunoglobulin Fab Fragments; D010455:Peptides; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic