Allospecific rat-mouse T hybridoma clones generated from kidney graft-infiltrating cells.
In order to dissect the cellular mechanisms that lead to allograft rejection, infiltrating cells can be isolated and expanded in vitro for functional assays. Since the maintenance of these lines and clones is time-consuming, and large numbers of specific cells cannot be easily obtained, we fused the graft infiltrating cells, after in vitro specific expansion, with the murine T lymphoma BW5147. The rat-mouse TT hybridomas thus generated were screened for antigen-dependent interleukin 2 production, for antigen-dependent polyclonal helper activity, and for surface phenotype. The high frequency of specific clones obtained indicates that this is a convenient approach to generate alloreactive hybridoma clones with specific functions from the inflammatory infiltrates of rejected grafts.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D006649:Histocompatibility Antigens / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D007668:Kidney / Q000276:immunology; D016030:Kidney Transplantation; D008213:Lymphocyte Activation; D051379:Mice; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000954:Antigens, Surface; D006649:Histocompatibility Antigens