Quantification of progesterone in human saliva.
A procedure for the quantification of progesterone in saliva has been developed, using gas chromatography/mass spectrometry (GC/MS) with selected ion monitoring of the 3-enol heptafluorobutyrate derivative. (2H2)Progesterone is used as internal standard. The detection limit (c. 10 pg ml-1) is sufficiently low to permit the determination of the steroid in the saliva of adolescent girls. Assessment of the procedure indicated good assay linearity and excellent accuracy in the determination of progesterone added to saliva. Comparison of GC/MS and radioimmunoassay data showed good agreement, substantiating earlier immunoassay evidence for the occurrence of episodic increases in salivary progesterone concentrations in perimenarcheal girls.
D000293:Adolescent; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008572:Menarche; D011374:Progesterone / Q000032:analysis*; D011863:Radioimmunoassay; D012463:Saliva / Q000032:analysis*
D011374:Progesterone