Quantification of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 1-methyl-4-phenylpyridinium ion in brain tissue by gas chromatography/mass spectrometry.
A sensitive gas chromatographic/mass spectrometric assay has been developed to quantify 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium ion (MPP+) in brain tissue. MPTP was derivatized with isobutyl chloroformate and then analyzed using gas chromatography/mass spectrometry. MPP+, a known metabolite of MPTP, was reduced with NaB2H4 to 2H2-MPTP before derivatization. Deuterated isotopomers of MPTP and MPP+ were used as internal standards. The intra-assay coefficients of variation in the determination of 57.3 ng MPTP and 371.8 ng MPP+ in 100 mg of mouse brain tissue were 3.0% and 5.6% (n = 7), respectively. The detection limit corresponds to about 2 ng per sample of authentic MPTP.
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015655:1-Methyl-4-phenylpyridinium; D000818:Animals; D001923:Brain Chemistry; D008401:Gas Chromatography-Mass Spectrometry; D000882:Haplorhini; D011725:Pyridines / Q000032:analysis*; D011726:Pyridinium Compounds / Q000032:analysis*; D013696:Temperature
D011725:Pyridines; D011726:Pyridinium Compounds; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D015655:1-Methyl-4-phenylpyridinium