Production of monoclonal antibodies to the rat insulin-like growth factor II (IGF-II) receptor.
BALB/c mice were immunized with rIGF-II receptors purified from 18-54, SF cells by chromatography of solubilized receptors over agarose-immobilized rIGF-II. Two fusions of splenic lymphocytes with FO mouse myeloma cells yielded 27 stable hybrids which were positive by ELISA. Cloning of seven of these hybrids yielded 30 positive clones by ELISA. At least seven of these clones (minimum of one from each parent hybrid) were capable of specifically immunoprecipitating the rIGF-II receptor.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D051381:Rats; D011972:Receptor, Insulin / Q000276:immunology; D017451:Receptors, Somatomedin; D013002:Somatomedins / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D017451:Receptors, Somatomedin; D013002:Somatomedins; D011972:Receptor, Insulin