[The study on human placental DHA-S transport mechanism (using placental microvillous membrane vesicles)].
To investigate the placental DHA-S (dehydroepiandrosterone sulfate) transport mechanism, the uptake of DHA-S into microvillous membrane vesicles prepared from human term placenta was studied using the rapid filtration technique. 1. The uptake of DHA-S into microvillous membrane vesicles was not dependent on both Na+ electrochemical gradient and membrane potential difference. 2. The uptake of DHA-S into microvillous membrane vesicles was dependent on temperature. The initial uptake rate of DHA-S at 37 degrees C was three times as great as at 4 degrees C. 3. The initial rate of DHA-S transport exhibited saturation kinetics with respect to the DHA-S concentration; an apparent Km of 0.067 mM and Vmax of 1.01 nmol/mg protein/20 sec were calculated. 4. The uptake of DHA-S into microvillous membrane vesicles was inhibited by DHA, but not by estriol. These results indicated that placental DHA-S transport was carrier mediated and a passive one.
D001692:Biological Transport; D003687:Dehydroepiandrosterone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D019314:Dehydroepiandrosterone Sulfate; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008871:Microvilli / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D013696:Temperature
D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate