[Determination of interleukin 1].
Interleukin 1 is an essential factor of macrophage dependent T cell activation. As a method for determining IL 1 the estimation of its comitogenic activity is used with mouse thymocytes. For preparation IL 1 standards the macrophage cell line P388D1 or human leukocytes are stimulated with LPS. The mitogenic activity of the thymocytes is tested in five different mouse strains; LPS does not disturb the IL 1 determination in the concentration range between 10(2) micrograms ml-1 and 10(-3) micrograms ml-1. Contrary to the Con A reactivity the susceptibility of the thymocytes on IL 1 is developing more slowly. The age of the animals for preparation thymocytes should not be under 8 to 10 weeks. IL 1 and IL 2 inhibitors must be considered in determining IL 1.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004926:Escherichia coli; D006801:Humans; D007158:Immunologic Techniques; D007375:Interleukin-1 / Q000032:analysis*; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D007375:Interleukin-1; D008070:Lipopolysaccharides; D008934:Mitogens
Zur Bestimmung von Interleukin 1.