High-sensitivity radioimmunoassay screening method for fentanyl.
A radioiodinated analog of fentanyl was synthesized for use with a commercially available radioimmunoassay for fentanyl. The sensitivity of the modified assay was at least 100 times greater than that of the original assay. Using this modified assay, concentrations of fentanyl as low as 1 pg/ml of fentanyl or fentanyl equivalents in equine urine were detected. Doses of fentanyl 100 times smaller than the minimum dose for a pharmacologic effect were detectable and a pharmacologically effective dose of fentanyl was detectable for up to 96 hours or more. The high sensitivity of the assay indicated that large numbers of urine samples (ie, 10 to 20) probably could be pooled and screened simultaneously, which would result in an economical analysis for fentanyl in the urine of horses after a race. Sufentanil and its metabolites also were detectable, using this assay, but at only about 1% of the efficiency at which fentanyl was detectable.
D000818:Animals; D005283:Fentanyl / Q000031:analogs & derivatives / Q000652:urine*; D006736:Horses / Q000652:urine*; D011863:Radioimmunoassay / Q000662:veterinary; D011933:Reagent Kits, Diagnostic; D017409:Sufentanil
D011933:Reagent Kits, Diagnostic; D017409:Sufentanil; D005283:Fentanyl