Rapid isolation and characterization of hybridization selected recombinants from lambda genomic libraries.
This paper describes an efficient protocol for the screening of lambda genomic libraries, plaque and DNA purification, and probe characterization by a combination of new and recently described techniques. The protocol has allowed large numbers of human subchromosome-specific probes to be rapidly generated from an EMBL3 library of human-mouse somatic cell hybrid DNA. The protocol affords considerable savings in time and effort over previous procedures.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D051379:Mice; D011995:Recombination, Genetic
D004274:DNA, Recombinant