The cytotoxic T-cell response to Ia antigens: lack of correlation between the level of specific cytotoxicity obtained in primary mixed lymphocyte reaction and the frequency of specific cytotoxic T lymphocyte.
We have investigated the cytotoxic response to allogeneic I-E and I-A antigens in bulk culture and limiting dilution experiments. A high degree of specific killing could be generated from unprimed T cells of I-E-negative strains upon stimulation with cells expressing I-E. In such conditions, cytotoxic T lymphocytes (CTL) were generated in the absence of a specific proliferative response. The frequency determinations by means of limiting dilution experiments showed that I-E-specific CTL precursors were much less frequent than the K and D specific precursors. The results suggest the existence of a population of I-E-specific CTL that failed to grow under conditions that allowed the growth of class I-specific CTL.
D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II