Binding sites for [3H]dopamine and dopamine-antagonists on cultured astrocytes of rat striatum and spinal cord: an autoradiographic study.
The cellular localization of binding sites for [3H]dopamine, and dopamine-antagonists (D1 and D2) was studied in organotypic cultures of rat striatum and spinal cord by means of autoradiography. In both types of cultures, many astrocytes were labelled by [3H]dopamine, the D1-antagonist [3H]cis-flupenthixol and the D2-antagonists [3H]domperidone and [3H]spiperone (10(-9) to 10(-8) M). Addition of unlabelled dopamine and antagonists at high concentrations (10(-6) to 10(-4) M) inhibited or markedly reduced binding of the radioligands indicating 'specific' binding of the compounds. Our autoradiographic studies are consistent with biochemical investigations by other authors, suggesting that astrocytes possess receptors for dopamine.
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001345:Autoradiography; D001665:Binding Sites; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D004294:Domperidone / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D018492:Dopamine Antagonists; D005475:Flupenthixol / Q000378:metabolism; D007650:Ketanserin; D010880:Piperidines / Q000494:pharmacology; D051381:Rats / Q000196:embryology; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013134:Spiperone / Q000378:metabolism; D013237:Stereoisomerism; D014316:Tritium
D018492:Dopamine Antagonists; D010880:Piperidines; D014316:Tritium; D013134:Spiperone; D004294:Domperidone; D007650:Ketanserin; D005475:Flupenthixol; D004298:Dopamine