Fibrinogen-induced erythrocyte aggregation: erythrocyte-binding site in the fibrinogen molecule.
The effect of fibrinogen and fibrinogen-derived products on the velocity of rouleau formation of human erythrocytes was quantitatively examined with a rheoscope combined with a video-camera, an image analyzer and a computer. (i) The velocity of rouleau formation by naturally occurring low-molecular-weight fibrinogen of 305 kDa and by desialylated fibrinogen was the same as that by native fibrinogen of 340 kDa. (ii) Concerning fibrinogen degradation products by plasmin, the velocity of rouleau formation decreased upon going from fibrinogen greater than fragment X greater than fragment Y (the ratio of molar concentration of fibrinogen, fragment X and fragment Y for giving a certain velocity of rouleau formation was approx. 1:2:5). The effect of fragments X and Y on the fibrinogen-induced rouleau formation was additive. (iii) Fragments D and E could not induce rouleau formation and did not affect the fibrinogen-, fragment X- and fragment Y-induced rouleau formation. (iv) Fibrinopeptides A and B and artificial tetrapeptides (Gly-Pro-Arg-Pro and Gly-His-Arg-Pro) did not affect the fibrinogen-induced rouleau formation. (v) The possible erythrocyte-binding site in fibrinogen molecule for leading to rouleaux was proposed to be in A alpha-chain (probably, around residues No. 207-303) near the terminal domain of the trinodular structure of fibrinogen.
D001665:Binding Sites; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D005338:Fibrin Fibrinogen Degradation Products / Q000494:pharmacology*; D005340:Fibrinogen / Q000378:metabolism / Q000494:pharmacology*; D005341:Fibrinolysin / Q000378:metabolism; D005344:Fibrinopeptide A / Q000494:pharmacology; D005345:Fibrinopeptide B / Q000494:pharmacology; D006801:Humans; D008297:Male; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids
D005338:Fibrin Fibrinogen Degradation Products; D012794:Sialic Acids; C015214:fibrinogen D fragment; C018543:fibrinogen fragment E; C030568:fibrinogen fragment X; C036129:fibrinogen fragment Y; D005344:Fibrinopeptide A; D005345:Fibrinopeptide B; D005340:Fibrinogen; D005341:Fibrinolysin; D019158:N-Acetylneuraminic Acid