Induction of an immature T-cell phenotype in malignant helper T cells by cocultivation with epidermal cell cultures.
The possible inductive effect of epidermal cells on T-cell maturation has been examined employing an in vitro cocultivation technique. Mononuclear cells from 6 patients with cutaneous T-cell lymphoma (CTCL) and from 12 healthy volunteers were studied. In the 6 CTCL patients, all showed an expansion of the helper T-cell subpopulation and in one patient with leukemic CTCL, there was almost complete replacement of peripheral blood mononuclear cells by malignant cells with a helper T-cell phenotype. Epidermal cells derived from normal human skin were cultured to confluent monolayers, and were cocultivated with the mononuclear cells from CTCL patients or normal controls for 48 h at a density of 10(6)/ml. Following cocultivation, the surface phenotype of the cells from the 12 healthy volunteers and 5 of the patients with CTCL showed no significant phenotypic change. In the patient with leukemic CTCL, however, the surface phenotype of the malignant T cells had changed, with the acquisition of the T6 antigen by the majority of the cells. Cells cocultivated in medium alone and with human fibroblast monolayers showed no change in surface phenotype. The malignant T cells from the leukemic CTCL patient failed to react in a mixed lymphocyte culture to lymphocytes from 2 different healthy donors, and showed no phenotypic change following culture with these lymphocytes, indicating that the phenotypic change seen was not due to allogeneic stimulation.
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002454:Cell Differentiation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D007801:Langerhans Cells / Q000166:cytology; D007959:Lymphocyte Culture Test, Mixed; D008223:Lymphoma / Q000473:pathology*; D012878:Skin Neoplasms / Q000473:pathology; D013601:T-Lymphocytes / Q000166:cytology*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology*
D000945:Antigens, Differentiation, T-Lymphocyte