title: Induction of the CD4-8+ suppressor phenotype in CD4+8+ human thymocytes by phorbol myristate acetate.
abstract: Human thymocytes were separated according to differential agglutination by peanut lectin (PNA). In the fractions obtained, the distribution and quantitative expression of CD antigens was determined by two-color fluorescence flow cytometry. A major population of CD4+8+ and minor populations of CD4+8- and CD4-8+ cells existed in unfractionated thymocytes. Agglutination of human thymocytes by PNA led to a highly effective enrichment of the CD4+8+ phenotype in the PNA+ thymocytes, and left three distinct phenotypes, CD4+8+, CD4+8-, and CD4-8+, in the PNA-thymocytes. After treatment of the CD4+8+ cells in both PNA+ and PNA- fractions for 20 hr with phorbol 12-myristate 13-acetate (PMA), 60%-90% of the cells expressed the CD4-8+ phenotype, whereas the CD4+8+ phenotype was decreased to 1%-3% of the population. In addition, pretreatment of both PNA+ and PNA- thymocytes with PMA induced suppressor activity in these cells, as shown by inhibition of immunoglobulin secretion by pokeweed mitogen (PWM)-stimulated peripheral blood lymphocytes (PBL).
authors.affiliation: Department of Pediatrics, Hokkaido University School of Medicine, Sapporo.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakiyama
authors.forename: A
authors.initials: A
authors.lastname: Ishizaka
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Ariga
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte; D002675:Child, Preschool; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D066298:In Vitro Techniques; D007223:Infant; D037102:Lectins; D019887:Peanut Agglutinin; D010641:Phenotype; D011971:Receptors, Immunologic / Q000187:drug effects; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D007136:Immunoglobulins; D037102:Lectins; D019887:Peanut Agglutinin; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D013755:Tetradecanoylphorbol Acetate