title: Analysis of lettuce necrotic yellows virus structural proteins with monoclonal antibodies and concanavalin A.
abstract: Three major structural proteins of lettuce necrotic yellow virus (LNYV) were identified by discontinuous polyacrylamide gel electrophoresis (PAGE) to have Mr approximately 78,000 (G), 57,000 (N), and 19,000 (M). Unreduced G and M proteins had faster mobilities in PAGE indicating the presence of disulfide bonds. The G protein was shown to be glycosylated with a complex network of oligosaccharides containing beta-N-acetylchitobiose N-linked to asparagine residues of the protein. Up to 17 additional minor bands were also detected in silver-stained electrophoretograms. In Western immunoblots, 9 of these (Mr approximately 27,000-220,000) were recognized by a monoclonal antibody to the N protein and another 6 (Mr approximately 58,000-180,000) with a monoclonal antibody to the G protein, indicating that they were degradation products or aggregates of these two viral proteins. Two minor silver-stained bands failed to react with either of the monoclonal antibodies, but were recognized by polyclonal anti-LNYV serum and are probably the L (Mr approximately 190,000) and NS (Mr approximately 38,000) viral proteins.
authors.affiliation: Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, South Australia.
authors.forename: R G
authors.initials: RG
authors.lastname: Dietzgen
authors.forename: R I
authors.initials: RI
authors.lastname: Francki
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology; D002499:Centrifugation, Density Gradient; D003208:Concanavalin A / Q000494:pharmacology; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D006031:Glycosylation; D008970:Molecular Weight; D010942:Plant Viruses / Q000276:immunology*; D012324:RNA-Dependent RNA Polymerase; D014758:Viral Core Proteins / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D003208:Concanavalin A; C040530:L protein, vesicular stomatitis virus; D012324:RNA-Dependent RNA Polymerase