title: A transforming activity not detected by DNA transfer to NIH 3T3 cells is detected by JB6 mouse epidermal cells.
abstract: Transfection of four different mouse epidermal tumor cell DNAs into NIH 3T3 cells yielded neither morphologically altered foci nor anchorage independence. However, promotion-sensitive, but not promotion-insensitive, JB6 mouse epidermal cell lines were permissive for the expression of anchorage independence after transfection of DNA from three of these tumor cell lines. This transforming activity and the promotion-sensitive activity that confers sensitivity to promotion of transformation show differences in restriction enzyme sensitivity. In view of this difference and the differences in both recipient cells and 12-O-tetradecanoyl-phorbol-13-acetate dependence of expression, it appears that the transforming activity and the promotion-sensitive activity are specified by different genes. The JB6 promotion-sensitive cell lines may be useful for detecting and cloning transforming genes that escape detection in the NIH 3T3 cell focus assay.
authors.forename: N H
authors.initials: NH
authors.lastname: Colburn
authors.forename: M I
authors.initials: MI
authors.lastname: Lerman
authors.forename: G A
authors.initials: GA
authors.lastname: Hegamyer
authors.forename: T D
authors.initials: TD
authors.lastname: Gindhart
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D003260:Contact Inhibition; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D000078404:Epidermal Cells; D051379:Mice; D010641:Phenotype; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes; D013755:Tetradecanoylphorbol Acetate