Search for cytomegalovirus in postmortem brain tissue from patients with Huntington's chorea and other psychiatric disease by molecular hybridization using cloned DNA.
Postmortem human brain extracts were examined for the presence of human cytomegalovirus (CMV) DNA by molecular hybridization using a dot blot technique. The method was able to detect picogram quantities of homologous DNA, but CMV specific hybridization was detected in only one of 83 brains examined. The positive case came from a patient who had received immunosuppressive therapy. We were not able to confirm the report that CMV is present in the brains of patients with Huntington's chorea, nor was CMV detected in the temporal cortex of brains from schizophrenic patients. Our findings are discussed in relation to the methodology for investigating a possible viral etiology of some neuropsychiatric diseases.
Taylor
G R
GR
Crow
T J
TJ
Higgins
T
T
Reynolds
G
G
D000328:Adult; D000368:Aged; D001921:Brain / Q000382:microbiology*; D002999:Clone Cells / Q000032:analysis; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D005625:Frontal Lobe / Q000032:analysis; D006801:Humans; D006816:Huntington Disease / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012367:RNA, Viral / Q000032:analysis; D012559:Schizophrenia / Q000382:microbiology
D004279:DNA, Viral; D012367:RNA, Viral