Sequence specific damage of DNA induced by reducing sugars.
Reducing sugars induced alkali-labile sites in DNA. The DNA reacted with D-fructose 6-phosphate or D-fructose in the presence of Cu2+ was cleaved by the treatment with aqueous piperidine at 90 degrees C for 30 min. Alkali-labile sites were induced frequently at the pyrimidine residues, especially at the pyrimidine residues in pyrimidine-purine (5'----3') sequences. Transition metal ions such as Cu2+ and oxygen radicals such as hydrogen peroxide were possibly involved in the induction of alkali-labile sites.
Morita
J
J
Ueda
K
K
Nanjo
S
S
Komano
T
T
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003300:Copper; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005636:Fructosephosphates; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship
D005636:Fructosephosphates; D003300:Copper; D004247:DNA; D004262:DNA Restriction Enzymes