Isolation and structural mapping of a human c-src gene homologous to the transforming gene (v-src) of Rous sarcoma virus.
We have utilized a lambda Charon 4A human genomic library to isolate recombinant clones harboring a highly conserved c-src locus containing nucleotide sequences homologous to the transforming gene of Rous sarcoma virus (v-src). Four overlapping clones spanning 24 kilobases of cellular DNA were analyzed by restriction endonuclease mapping. Human c-src sequences homologous to the entire v-src region are present in a 20-kilobase region that contains 11 exons as determined by restriction mapping studies utilizing hybridization to labeled DNA probes representing various subregions of the v-src gene and by preliminary DNA sequencing analyses. A considerable degree of similarity exists between the organization of the human c-src gene and that of the corresponding chicken c-src gene with respect to exon size and number. However, the human c-src locus is larger than the corresponding chicken c-src locus, because many human c-src introns are larger than those of chicken c-src. alu family repetitive sequences are present within several human c-src introns. This locus represents a highly conserved human c-src locus that is detectable in human cellular DNAs from various sources including placenta, HeLa cells, and WI-38 cells.
Gibbs
C P
CP
Tanaka
A
A
Anderson
S K
SK
Radul
J
J
Baar
J
J
Ridgway
A
A
Kung
H J
HJ
Fujita
D J
DJ
D001358:Avian Sarcoma Viruses / Q000201:enzymology / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D006801:Humans; D009693:Nucleic Acid Hybridization; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D011494:Protein Kinases / Q000235:genetics*; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics*
D004251:DNA Transposable Elements; D014764:Viral Proteins; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src); D004262:DNA Restriction Enzymes