Mononuclear cell-mediated modulation of synovial cell metabolism. I. Collagen synthesis.
Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-mediated. The active material, of apparent molecular weight 12 000-20 000, also displays the properties of the mononuclear cell factor (MCF) previously described by others, since its stimulates collagenase and PGE2 release by the cultured synovial cells. Furthermore, it co-purifies with interleukin 1 (IL 1) as shown by lymphocyte-activating factor activity. This strongly suggests that IL 1 could be responsible for some (or all) the effects observed on MCF-exposed synovial cells. From these data, we deduce the possibility that mononuclear cells may participate in limiting synovial collagen deposition in rheumatoid arthritis.
Pujol
J P
JP
Penfornis
H
H
Arenzana-Seisdedos
F
F
Bocquet
J
J
Farjanel
J
J
Rattner
A
A
Brisset
M
M
Virelizier
J L
JL
Beliard
R
R
Loyau
G
G
D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003470:Culture Media; D006602:Hexosyltransferases / Q000378:metabolism; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis; D008970:Molecular Weight; D009000:Monocytes / Q000502:physiology*; D015846:Monokines; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000502:physiology*; D013583:Synovial Membrane / Q000378:metabolism*
D003470:Culture Media; D007375:Interleukin-1; D007376:Interleukin-2; D015846:Monokines; D011506:Proteins; D003094:Collagen; D011393:Procollagen-Proline Dioxygenase; D006602:Hexosyltransferases; D007213:Indomethacin