In vitro culture of two populations (dividing and nondividing) of exoerythrocytic parasites of Plasmodium vivax.
Plasmodium vivax sporozoites invaded human hepatoma cells and differentiated into 2 types of exoerythrocytic (EE) parasites. One group was composed of actively dividing schizonts, which released merozoites after 9 days of culture. The second group was nondividing and persisted after the primary schizonts disappeared from the culture. EE schizonts progressively lost reactivity to monoclonal antibodies to the surface-protective protein antigen of P. vivax sporozoites, but the persisting parasites remained strongly reactive. In the 2 strains of P. vivax studied, the ratio of schizonts to persisting parasites was approximately equal.
Hollingdale
M R
MR
Collins
W E
WE
Campbell
C C
CC
Schwartz
A L
AL
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis; D001399:Azure Stains; D006528:Carcinoma, Hepatocellular; D002455:Cell Division; D002460:Cell Line; D006801:Humans; D008113:Liver Neoplasms; D010966:Plasmodium vivax / Q000166:cytology / Q000254:growth & development* / Q000276:immunology; D014617:Vacuoles / Q000469:parasitology
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D001399:Azure Stains