Hypochlorite radioiodination of parathyroid peptides (hPTH1-34, [Tyr43]hPTH44-68).
Parathyroid hormone (PTH) radioimmunoassays have conventionally utilized [125I]bPTH1-84 as a radioligand, but more region-specific PTH assays are now possible with the use of recently available synthetic PTH peptides as standards and radioligands. A radioiodination procedure has been developed that utilizes hypochlorite as an oxidant and that is capable of producing PTH tracers of high specific activity (200 to 250 microCi/micrograms), prolonged stability, and excellent immunologic potency. Radioiodinated hPTH1-34 and [Tyr43]hPTH44-68 produced by hypochlorite iodination techniques can be used to develop sensitive and region-specific PTH assays of use in clinical and research situations.
Orwoll
E
E
Kopel
A
A
Opsahl
Z
Z
Roberts
L
L
Endres
D
D
McClung
M
M
D011232:Chemical Precipitation; D002850:Chromatography, Gel; D006801:Humans; D006997:Hypochlorous Acid; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D010281:Parathyroid Hormone / Q000032:analysis / Q000097:blood; D010446:Peptide Fragments / Q000032:analysis; D011863:Radioimmunoassay; D011869:Radioligand Assay; D019379:Teriparatide
D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D019379:Teriparatide; D006997:Hypochlorous Acid; C044339:parathyroid hormone (44-68), 43-Tyr-