Distinct phenotypic composition of diffuse interstitial and perivascular focal infiltrates in renal allografts: a morphometric analysis of cellular infiltration under conventional immunosuppressive therapy and under cyclosporine A.
Phenotypic analysis of interstitial mononuclear cell infiltrates was undertaken in 40 transplant renal specimens obtained from 38 patients in order to assess the influence of immunosuppressive therapy. Thirteen patients were given conventional immunosuppressive treatment (azathioprine and prednisone) and the other 25 received cyclosporine. The immunostaining was performed using seven antileucocyte antibodies by alkaline phosphatase-anti-alkaline phosphatase method. Interstitial infiltrates were distributed in two patterns: diffuse infiltrates and periglomerular/perivascular aggregates. The phenotypic composition was distinct in these two patterns: in diffuse infiltrates, monocytes/macrophages (EBM 11) represented the predominant inflammatory cell and were associated with a minor component of T cells (T 11). In contrast, aggregates had a major T lymphocyte phenotype in addition with few foci of B cells. T4 subset of T lymphocytes always predominated over T8 subset. The repartition and the proportion of each cell type were not significantly different in rejecting and not rejecting grafts and were not affected by the immunosuppressive regimen.
Guettier
C
C
Research group for Vascular and Renal Morphology and Pathology, INSERM U 28, Broussais Hospital, Paris, France.
Nochy
D
D
Hinglais
N
N
Pelletier
L
L
Mandet
C
C
Bedrossian
J
J
Duboust
A
A
Moulonguet Doleris
L
L
Camilleri
J P
JP
Bariety
J
J
D000284:Administration, Oral; D000911:Antibodies, Monoclonal; D001379:Azathioprine / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000032:analysis; D002449:Cell Aggregation / Q000187:drug effects; D003524:Cyclosporins / Q000008:administration & dosage / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000473:pathology*; D007668:Kidney / Q000473:pathology; D016030:Kidney Transplantation; D007694:Killer Cells, Natural / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis*; D008264:Macrophages / Q000032:analysis*; D010641:Phenotype; D006377:T-Lymphocytes, Helper-Inducer / Q000032:analysis; D050378:T-Lymphocytes, Regulatory / Q000032:analysis
D000911:Antibodies, Monoclonal; D003524:Cyclosporins; D001379:Azathioprine