Detection of haematoporphyrin derivative and haematoporphyrin excited states in cell environments.
The photosensitiser currently used in the photodynamic therapy of cancer is haematoporphyrin derivative. Pulsed laser studies of this material and also of the "parent" molecule haematoporphyrin in polyoma-transformed fibroblast cells have now been studied. We report, for the first time, the observation of the triplet absorption of these sensitisers in cells. The corresponding triplet-triplet spectra are red shifted compared to aqueous buffer, lambda(max) 420 nm shifts to 460 nm. We also report our failure to observe singlet oxygen from the cells even though the triplet state can be seen to interact with the oxygen and even though singlet oxygen can be readily detected with the same sensitisers bound to serum albumin.
Truscott
T G
TG
Department of Chemistry, Paisley College of Technology, Scotland.
McLean
A J
AJ
Phillips
A M
AM
Foulds
W S
WS
D002460:Cell Line; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins; D010100:Oxygen / Q000032:analysis; D010782:Photolysis
D006415:Hematoporphyrins; D017324:Hematoporphyrin Derivative; D010100:Oxygen