title: Docosahexaenoate-containing molecular species of glycerophospholipids from frog retinal rod outer segments show different rates of biosynthesis and turnover.
abstract: We have studied the de novo synthesis and subsequent turnover of major docosahexaenoate-containing molecular species in frog rod outer segment (ROS) phospholipids following intravitreal injection of [2-3H]glycerol. On selected days after injection, ROS were prepared and phospholipids extracted. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS) were isolated and converted to diradylglycerols with phospholipase C. Diradylglycerols were derivatized with benzoic anhydride and resolved into diacylglycerobenzoates and ether-linked glycerobenzoates. The diacylglycerobenzoates were fractionated into molecular species by HPLC, quantitated, and counted for radioactivity. Label was incorporated into ROS phospholipids by day 1 and was followed up through the eighth day. The dipolyenoic species 22:6-22:6 from PC showed a 3-5 times higher radiospecific activity than the same species from either PE or PS. In PC, the specific activities of 16:0-22:6 and 18:0-22:6 were 3-5 times lower than the specific activity of 22:6-22:6. In contrast, for PE, the specific activities of 16:0-22:6 and 18:0-22:6 were 2-5 times higher than that of 22:6-22:6. The specific activities of 18:0-22:6 and 22:6-22:6 were similar in PS. Specific activities of the docosahexaenoate-containing species began approximating an exponential decline 6-8 days postinjection and continued through the 31st day. The rate of decline was determined by calculating the half-life of each molecular species, which was used as a measure of the turnover of the species. The species 22:6-22:6-PE and 18:0-22:6-PE showed a 2-3 times slower turnover rate than the corresponding species from either PC or PS.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: K
authors.initials: K
authors.lastname: Louie
authors.forename: R D
authors.initials: RD
authors.lastname: Wiegand
authors.forename: R E
authors.initials: RE
authors.lastname: Anderson
mesh_terms: D000818:Animals; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004281:Docosahexaenoic Acids / Q000032:analysis*; D005990:Glycerol / Q000378:metabolism; D007700:Kinetics; D010713:Phosphatidylcholines / Q000032:analysis / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000032:analysis / Q000378:metabolism*; D010718:Phosphatidylserines / Q000032:analysis / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000032:analysis*; D011894:Rana pipiens; D012374:Rod Cell Outer Segment / Q000032:analysis*
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D004281:Docosahexaenoic Acids; D005990:Glycerol