Identification of an HLA-DQ beta-chain related genomic sequence associated with insulin-dependent diabetes.
Restriction fragment length polymorphism detected by a cDNA probe for an HLA-DQ beta-chain gene has revealed a HLA-DR4 linked BamH1 3.7 kb fragment which is rarely found among insulin-dependent diabetic patients. The present analysis demonstrates that the BamH1 3.7 kb fragment present on a HLA-DR4 positive chromosome in a healthy individual contains coding sequences for an HLA-DQ beta-chain gene and that the absence of this fragment among HLA-DR4 positive insulin-dependent patients is due to the loss of a BamH1 restriction within an intervening sequence.
Michelsen
B
B
Kastern
W
W
Lernmark
A
A
Owerbach
D
D
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D008040:Genetic Linkage; D006683:HLA-DQ Antigens; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic
D006683:HLA-DQ Antigens; D000949:Histocompatibility Antigens Class II; D004262:DNA Restriction Enzymes