Induction of human interferon gamma in nylon-column nonadherent human lymphocyte cells by heat-treated poly I:poly C.
Human peripheral blood mononuclear leukocytes (PBML) were separated by Ficoll-hypaque gradient from human peripheral whole blood cells. The PBML were further separated by nylon-column. When nylon-column nonadherent cells (NNA cells) were induced by poly I:poly C which was prepared by heat-treatment, high levels of acid- and heat-labile interferon (IFN) were obtained. The IFN was not neutralized with human IFN-alpha or -beta anti-serum, but was completely neutralized with human IFN-gamma anti-serum, indicating that the IFN was human IFN-gamma.
Tamura
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M
Saito
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Sasakawa
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S
D002448:Cell Adhesion; D002850:Chromatography, Gel; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007369:Interferon Inducers; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human; D011070:Poly I-C / Q000494:pharmacology*
D007369:Interferon Inducers; D007371:Interferon-gamma; D011070:Poly I-C