Molecular cloning and expression in Streptomyces lividans of a streptomycin 6-phosphotransferase gene from a streptomycin-producing microorganism.
The gene encoding streptomycin 6-kinase involved in the self-resistance of the streptomycin-producing Streptomyces griseus HUT 6037 was cloned in the plasmid vector pIJ703. The resulting plasmid, pSP6, contained 2.5 kb inserts of S. griseus DNA. When streptomycin-susceptible S. lividans 1326 was retransformed with pSP6, all transformants produced streptomycin 6-kinase. Addition of streptomycin to the culture medium of S. lividans carrying pSP6 plasmid brought about a remarkable increase in streptomycin 6-kinase activity in the cell extracts. It is suggested from the results that the production of streptomycin 6-kinase in streptomycin producer was induced by streptomycin accumulated during cultivation.
Shinkawa
H
H
Sugiyama
M
M
Nimi
O
O
Nomi
R
R
D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004790:Enzyme Induction / Q000187:drug effects; D005798:Genes, Bacterial; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*; D013305:Streptomyces griseus / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022680:streptomycin 6-kinase; D013307:Streptomycin