title: SV40 chromatin structure is not essential for viral gene expression.
abstract: The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T- and V-antigen synthesis was demonstrable in nearly every recipient cell. Only linear DNA induced T-antigen synthesis with a very low efficiency after cytoplasmic injection. This low activity correlates with a rapid degradation of DNA III in the recipient cells. Further modifications observed immediately after injection are relaxation of superhelical molecules and formation of high-Mr DNA. Assembly of the injected DNA into SV40 chromatin-like structure, however, occurred only late after early viral gene expression.
authors.forename: A
authors.initials: A
authors.lastname: Graessmann
authors.forename: C
authors.initials: C
authors.lastname: Bumke-Vogt
authors.forename: G
authors.initials: G
authors.lastname: Buschhausen
authors.forename: M
authors.initials: M
authors.lastname: Bauer
authors.forename: M
authors.initials: M
authors.lastname: Graessmann
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D002460:Cell Line; D002522:Chlorocebus aethiops; D002843:Chromatin / Q000502:physiology*; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D005814:Genes, Viral; D007668:Kidney; D013539:Simian virus 40 / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D002843:Chromatin; D004279:DNA, Viral