title: Effect of chronic ethanol administration on enzyme and lipid properties of liver plasma membranes in long and short sleep mice.
abstract: Mechanisms of alcoholic liver disease are still ill defined. We evaluated in two outbred lines of mice whether chronic ingestion of ethanol alters the lipid composition and/or enzyme activity of liver plasma membranes. Two mouse lines with different sensitivities towards the hypnotic effect of ethanol, designated long sleep and short sleep, were fed a liquid diet containing ethanol for 30 days. Ethanol intake reached 30 gm per kg per day in both lines, and serum ethanol levels were similar. In addition, hepatic triglyceride levels were similarly increased 2-fold with ethanol feeding. The following effects of ethanol treatment were observed in liver plasma membrane fractions: (i) Na+,K+-ATPase was significantly increased to 26% above control in long sleep only; (ii) alkaline phosphatase activity was 2-fold increased in both lines; (iii) 5'-nucleotidase, leucine aminopeptidase and Mg2+-ATPase activities remained unchanged in both lines; (iv) unesterified cholesterol and total phospholipid contents were unaltered in both lines, and (v) cholesteryl esters were increased in both lines, but to a greater extent in short sleep (1.5 vs. 4-fold). Thus, chronic ethanol ingestion induces specific alterations in liver plasma membrane structure and function, suggesting that adaptive responses to ethanol may be determined in part by inherited factors.
authors.forename: T
authors.initials: T
authors.lastname: Zysset
authors.forename: M A
authors.initials: MA
authors.lastname: Polokoff
authors.forename: F R
authors.initials: FR
authors.lastname: Simon
mesh_terms: D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008108:Liver Diseases, Alcoholic / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D009708:Nucleotidases / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D012890:Sleep / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D002788:Cholesterol Esters; D008563:Membrane Lipids; D010743:Phospholipids; D014280:Triglycerides; D000431:Ethanol; D002784:Cholesterol; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D007931:Leucyl Aminopeptidase; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase