Rabbit anti-idiotypic antibodies raised against monoclonal anti-morphine IgG block mu & delta opiate receptor sites.
Rabbit antibodies have been raised against murine monoclonal anti-morphine Fab fragments. Following affinity purification, these antibodies competitively inhibit morphine binding to anti-morphine monoclonal antibodies, yet do not recognize normal mouse IgGs, suggesting that the antibodies produced are anti-idiotypic with respect to the anti-morphine IgG. More importantly, the purified antibodies competitively inhibit binding of morphine, naloxone, and D-ala-2-D-leu-5-enkephalin to rat brain opiate receptors.
Glasel
J A
JA
Myers
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WE
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000276:immunology; D016308:Enkephalin, Leucine-2-Alanine; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D009020:Morphine / Q000276:immunology*; D009270:Naloxone / Q000276:immunology; D011817:Rabbits; D051381:Rats; D011957:Receptors, Opioid / Q000276:immunology*; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D009270:Naloxone; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; D009020:Morphine