Nuclear location of the p55 subunit of the IL-2 receptor following activation of the HT-2 T helper cell line.
After mitogenic or antigenic stimulation, T cells express interleukin-2 receptors (IL-2R). The mechanism and control of signal transduction following binding of IL-2 to IL-2R are poorly understood. Using two rat monoclonal antibodies (5A2 and 7D4) specific for two distinct epitopes of the p55 subunit of mouse IL-2R, we have studied the cellular localization of this molecule by immunocytochemistry during the IL-2-mediated activation of mouse T helper cell clone HT-2. During the activation cycle, nuclear staining for the p55 subunit of the IL-2 receptor was transiently observed. It is suggested that the transient nuclear location of the IL-2R may play a critical role in the control of T-cell activation, proliferation and/or differentiation.
Jothy
S
S
Department of Pathology, McGill University, Montreal, Quebec.
Abadie
A
A
Froussard
P
P
Duphot
M
M
Thèze
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D008213:Lymphocyte Activation; D051379:Mice; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015375:Receptors, Interleukin-2