Further characterization of [3H] flunitrazepam binding sites on cultured mouse astroglia.
Astroglial cells in primary cultures bind [3H]flunitrazepam with a high affinity on a single type of site and on a number of binding sites which increased during astroglial growth and differentiation. These binding sites show a particular pharmacological spectrum characterized by an inhibition of high affinity by RO-5-4864 (4'-chlorodiazepam), an anticonvulsant of the benzodiazepine family and by an inhibition of binding of lower affinities by diazepam clonazepam and clobazam. RO-5-4864 and clonazepam compete for the same binding site in astroglia. The heat stability and the hormonal modulation by thyroxine are similar for astroglia and neuronal-cells. Benzodiazepines modulate the astroglial 5-HT receptor. Such an effect could be a possible physiological response to benzodiazepines for astroglial cells in primary cultures.
Tardy
M
M
Fages
C
C
Dupre
G
G
Costa
M F
MF
Bardakdjian
J
J
Rolland
B
B
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001665:Binding Sites; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D003975:Diazepam / Q000494:pharmacology; D005445:Flunitrazepam / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D008566:Membranes / Q000378:metabolism; D051379:Mice; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism
D008024:Ligands; D011963:Receptors, GABA-A; D012701:Serotonin; D005445:Flunitrazepam; D003994:Bucladesine; D003975:Diazepam