Characterization of the DNA of the hamster papovavirus: II. A comparison of binding sites for Escherichia coli- and calf thymus RNA polymerase II on the hamster papovavirus genome.
Calf thymus (CT) RNA polymerase II bound to hamster papovavirus (HaPV) DNA was visualized by electron microscopy and compared to binding sites obtained after binding of Escherichia coli RNA polymerase to the HaPV genome. Thirteen binding sites were observed with CT polymerase II at map positions 0.04-0.07; 0.11; 0.18; 0.30; 0.37; 0.47; 0.57; 0.65; 0.78; 0.83; 0.90 and 0.94 using the unique BamHI cleavage site as zero point on the HaPV physical map. These binding sites correlate well with A + T rich sequences within the HaPV DNA as revealed by experiments using protein 32 coded for by phage T4. A comparison of binding of prokaryotic and eukaryotic RNA polymerase demonstrates a high degree of correspondence for most of the binding sites on the HaPV genome.
Vogel
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Scherneck
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D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D006224:Cricetinae; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology*; D005814:Genes, Viral; D027383:Papillomaviridae / Q000032:analysis* / Q000235:genetics; D027382:Polyomaviridae; D012319:RNA Polymerase II / Q000378:metabolism*; D013950:Thymus Gland / Q000201:enzymology
D004279:DNA, Viral; D012319:RNA Polymerase II; D004262:DNA Restriction Enzymes