Stimulation of glucose uptake by transforming growth factor beta: evidence for the requirement of epidermal growth factor-receptor activation.
Transforming growth factor beta (TGF-beta), derived from human platelets, stimulates the uptake of 2-deoxy-glucose by cultured cell monolayers 2- to 4-fold. Stimulation can be detected as early as 30 min with as little as 0.1 ng of TGF-beta per ml and maximal effects can be obtained at 2 hr with 1 ng of the growth factor per ml. TGF-beta-induced stimulation of sugar uptake is enhanced by the co-addition of platelet-derived growth factor (10 ng/ml) or epidermal growth factor (EGF, 1 ng/ml). The NR-6 variant of mouse 3T3 cells, which lack EGF receptors, is not stimulated by TGF-beta. Antisera to EGF receptors that block 125I-labeled EGF binding also inhibit TGF-beta stimulation of 2-deoxyglucose uptake, although 125I-labeled TGF-beta binding remains unimpaired. In contrast, anti-sera to the EGF receptor, which do not block EGF binding, have no measurable effect on the TGF-beta-stimulated uptake of 2-deoxyglucose. We confirm that the receptor for TGF-beta is distinct from the receptor for EGF and we conclude that TGF-beta stimulation of 2-deoxyglucose uptake requires the co-activation of the EGF receptor kinase system.
Inman
W H
WH
Colowick
S P
SP
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors; D005347:Fibroblasts / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology*; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000502:physiology*; D015291:Transforming Growth Factors
D006133:Growth Substances; D010455:Peptides; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D003847:Deoxyglucose; D011494:Protein Kinases; D066246:ErbB Receptors; D005947:Glucose