Characterization of macrophage adhesion molecule.
Macrophage adhesion molecule (MAM), an abundant surface molecule which functions in the adhesion and spreading of guinea pig macrophages on surfaces, is characterized as a heterodimer of the trypsin- and plasmin-sensitive glycopeptide gp160 (MAM-alpha) and the glycopeptide gp93 (MAM-beta). The density of MAM molecules is estimated at 630,000 per macrophage on the basis of quantitative binding of 125I-labeled monoclonal antibody. The glycopeptide subunits display microheterogeneity on isoelectrofocusing; the pI is 5.8-6.3 for gp160 (MAM-alpha) and 6.4-7.0 for gp93 (MAM-beta). A neutrophil gp160, gp93 molecule was shown to be indistinguishable from macrophage MAM on the basis of electrophoresis, isoelectrofocusing, and reactivity with 10 monoclonal antibodies. A related heterodimer of gp93 associated with a larger, antigenically different glycopeptide (gp180,gp93) was identified on circulating lymphocytes. Cumulative properties indicate that MAM is the guinea pig analogue of human Mo1 and mouse Mac-1.
Remold-O'Donnell
E
E
Center for Blood Research, Boston, Massachusetts 02115.
Savage
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000302:isolation & purification*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D004591:Electrophoresis, Polyacrylamide Gel; D005341:Fibrinolysin; D006020:Glycopeptides / Q000302:isolation & purification; D006168:Guinea Pigs; D008214:Lymphocytes / Q000276:immunology; D046911:Macromolecular Substances; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D008297:Male; D008970:Molecular Weight; D009504:Neutrophils / Q000276:immunology; D014357:Trypsin
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D006020:Glycopeptides; D046911:Macromolecular Substances; D014357:Trypsin; D005341:Fibrinolysin