title: Nucleotide sequence of the gene for the gamma chain of human fibrinogen.
abstract: A human genomic DNA library was screened for the gene coding for the gamma chain of fibrinogen by using a human cDNA for the gamma chain as a hybridization probe. The gene was identified in three overlapping recombinant lambda bacteriophage, and its sequence, including the immediate 5' and 3' flanking regions, was determined. The DNA sequence analysis revealed the presence of 10 exons coding for 411 amino acids present in the mature protein and a signal sequence of 26 amino acids. Two 30 base pair (bp) direct repeats of 93% identity were found 468 bp upstream from the transcription initiation site. The DNA sequence of the gene for the gamma chain of human fibrinogen showed considerable sequence homology with a partial sequence reported for the gene for the gamma chain of rat fibrinogen.
authors.forename: M W
authors.initials: MW
authors.lastname: Rixon
authors.forename: D W
authors.initials: DW
authors.lastname: Chung
authors.forename: E W
authors.initials: EW
authors.lastname: Davie
mesh_terms: D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D005340:Fibrinogen / Q000235:genetics*; D005796:Genes; D006801:Humans; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; C027539:fibrinopeptides gamma; D005340:Fibrinogen; D004247:DNA; D004262:DNA Restriction Enzymes