Three-dimensional reconstruction of maltoporin from electron microscopy and image processing.
Two dimensional crystals of maltoporin (or phage lambda receptor) were obtained by reconstitution of purified maltoporin trimers and Escherichia coli phospholipids by detergent dialysis. Two different trimer packing forms were observed. One was hexagonal (a = 7.8 nm) and one rectangular (a = 7.8 nm, b = 13.6 nm). In this paper we describe the three-dimensional structure of maltoporin, deduced from the study of the rectangular form by electron microscopy and image processing. At a resolution of approximately 2.5 nm, maltoporin trimers form aqueous channel triplets which appear to merge into a single outlet at the periplasmic surface of the outer membrane. The pore defined by maltoporin has a similar structure to that outlined by the matrix protein. From the results of functional studies by conductance measurement, it is concluded that the three channels defined by maltoporin act, contrary to those formed by the porin (OmpF protein), as a single conducting unit. A tentative outline of the maltoporin promoter is given. Maltoporin appears to be constituted by three different domains: a major rod-like domain spanning the membrane, a minor domain located near the periplasmic surface of the membrane and finally a central domain responsible for the splitting of the channel.
Lepault
J
J
European Molecular Biology Laboratory, Heidelberg, FRG.
Dargent
B
B
Tichelaar
W
W
Rosenbusch
J P
JP
Leonard
K
K
Pattus
F
F
D001425:Bacterial Outer Membrane Proteins; D010582:Bacteriophage lambda / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D010743:Phospholipids / Q000378:metabolism; D018272:Porins; D011487:Protein Conformation; D011991:Receptors, Virus / Q000302:isolation & purification / Q000378:metabolism; D012984:Software
D001425:Bacterial Outer Membrane Proteins; D046911:Macromolecular Substances; D010743:Phospholipids; D018272:Porins; D011991:Receptors, Virus; C016532:maltoporins