Angiofollicular lymph node hyperplasia associated with myelofibrosis case report and immunologic marker studies.
A case of angiofollicular lymph node hyperplasia (AFH) (hyaline vascular type) of the mediastinum associated with myelofibrosis is reported with immune marker studies. We hypothesize that the myelofibrosis is more than a mere coincidence since other myeloproliferative disorders have been observed in conjunction with AFH. Immunohistochemistry on cryostat sections of lymph node using the biotin-avidin fluorescence technique showed the presence of numerous T helper cells and very few T suppressor/cytotoxic cells in the parafollicular areas. Concentric rings of lymphocytes showed the phenotype of mantle zone lymphocytes (i.e. IgM, IgD positivity), while the germinal centres were ill-formed as shown by Leu 14 (a Pan B cell marker) staining. By electron microscopy, follicular dendritic reticulum cells were readily found. The above findings suggest an abnormality of germinal center formation.
Bleiweiss
I J
IJ
Laboratory Service, Bronx Veterans' Administration Medical Center, NY 10468.
Dumitrescu
O L
OL
Jagirdar
J
J
D000943:Antigens, Differentiation / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology; D005871:Castleman Disease / Q000276:immunology / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D006965:Hyperplasia; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D055728:Primary Myelofibrosis / Q000473:pathology*; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology
D000943:Antigens, Differentiation