Distribution of G + C-rich regions in varicella-zoster virus DNA.
The distribution of G + C-rich sequences in the genome of varicella-zoster virus (VZV) was investigated by partial denaturation, equilibrium sedimentation and Southern blot analyses. Portions of the IRS and TRS repeat sequences bounding the US region of the DNA were found to have a G + C content 10 to 20% greater than the overall 47% G + C content of the VZV genome. A stretch of DNA (approx. 1500 base pairs) at the UL-IRS junction and repeated at the terminus of the TRS sequences was found to be about 64% G + C, based on sedimentation equilibrium measurements. We also report the cloning of a novel fragment containing sequences from both the UL and TRS termini of the VZV genome. Our ability to clone this fragment suggests that unusual forms of VZV DNA including closed circular molecules and molecules with an inverted UL region can be packaged into nucleocapsids.
Ruyechan
W T
WT
Casey
T A
TA
Reinhold
W
W
Weir
A C
AC
Wellman
M
M
Straus
S E
SE
Hay
J
J
D001482:Base Composition; D001483:Base Sequence; D003596:Cytosine / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D006147:Guanine / Q000032:analysis*; D014645:Herpesvirus 3, Human / Q000235:genetics*; D009691:Nucleic Acid Denaturation
D004279:DNA, Viral; D006147:Guanine; D003596:Cytosine; D004262:DNA Restriction Enzymes