Glycosidases in normal and regenerating chicken liver, hepatoma Mc-29, Rous sarcoma, in turkey poult liver and hemocytoblastomes, provoked by the leukosis virus strain Mc-31.
Four glycosidases (beta-galactosidase, alpha-mannosidase, alpha-fucosidase and beta-N-acetylglucosaminidase) were studied in chicken normal and regenerating liver, in turkey poult liver and in virus induced avian tumors--chicken hepatoma (strain Mc-29), Rous sarcoma (strain Schmidt-Ruppin) and turkey poult hemocytoblastoma nodules (strain Mc-31). The multiple forms of beta-N-acetylglucosaminidase were assayed as well. A particular enzyme pattern was found in the tumor lines under investigation. A characteristic property of hepatoma cells was the elevation of beta-galactosidase activity and of the former enzyme and that of beta-N-acetylglucosaminidase for the hemocytoblastoma. In Rous sarcoma the glycosidase activities (except that of alpha-fucosidase) were much lower, compared to the other two solid tumors. All enzyme activities were compared with those in the normal liver of the corresponding avian species, and with the liver of tumor bearing fowls and with regenerating chicken liver. Unlike the rat liver in the avian normal and tumor tissues the percentual ratio between the multiple forms A and B of beta-N-acetylglucosaminidase was found to be 30:70%.
Chelibonova-Lorer
H
H
Ivanov
S
S
Gavazova
E
E
Antonova
M
M
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001353:Avian Leukosis / Q000201:enzymology*; D002645:Chickens / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000209:etiology; D008115:Liver Regeneration; D008361:Mannosidases / Q000378:metabolism; D051381:Rats; D001357:Sarcoma, Avian / Q000201:enzymology*; D014422:Turkeys / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000378:metabolism; D043323:alpha-Mannosidase; D001616:beta-Galactosidase / Q000378:metabolism
D006026:Glycoside Hydrolases; D008361:Mannosidases; D001616:beta-Galactosidase; D043323:alpha-Mannosidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase