Sialosyllactotetraosylceramide, a novel ganglioside antigen detected in human carcinomas by a monoclonal antibody.
A novel ganglioside was detected in a small cell lung carcinoma by TLC-immunostaining of gangliosides with a monoclonal antibody, the C-50 MAb. Structural characterization showed this ganglioside to be IV3NeuAc-LcOse4Cer, a hitherto unknown ganglioside. This ganglioside has also been detected as a minor component in many different carcinomas using the C-50 MAb. The normally dominant CA-50 ganglioside antigen is IV3NeuAc, III4Fuc-LcOse4Cer. Based upon solid-phase binding to IV3NeuAc, III4-LcOse4Cer and IV3NeuAc-LcOse4Cer it is concluded that the C-50 MAb recognizes an epitope present in sialylated type I carbohydrate chains.
Nilsson
O
O
Månsson
J E
JE
Lindholm
L
L
Holmgren
J
J
Svennerholm
L
L
D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D018288:Carcinoma, Small Cell / Q000032:analysis*; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis* / Q000276:immunology; D005915:Globosides; D006801:Humans; D007158:Immunologic Techniques; D008175:Lung Neoplasms / Q000032:analysis*; D051379:Mice
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D005732:Gangliosides; D005915:Globosides; C037790:sialosylgloboside