An empirical method for the evaluation of the quality of genomic DNA libraries.
An empirical method is presented that enables one to determine, with only a relatively small investment of time and materials, whether a lambda genomic DNA library will be a productive source of clones carrying specific sequences. The method provides an indication of the abundance of the sequences in library DNA and indicates whether major DNA rearrangements have occurred during library construction and amplification.
Phillips
S L
SL
Casavant
N C
NC
Hutchison
C A
CA
Edgell
M H
MH
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005784:Gene Amplification; D005914:Globins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization
D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes