title: Generation of lymphomagenic mouse type-C viruses from radiation- or chemically-induced non-producer T-cell lymphoma cell lines infected with non-oncogenic ecotropic virus.
abstract: Highly lymphomagenic mouse type-C viruses were generated from radiation- or chemically-induced T-cell lymphoma cell lines of NFS/N mouse origin infected with a non-oncogenic ecotropic virus E4. By analysis of these progeny viruses, the following results were obtained. 1) The viruses were lymphomagenic in neonatally inoculated NFS/N and C3H/He mice and W/Fu rats but not in Balb/c and C57BL/6N mice, indicating that they possess the Fv-1n tropism of exogenously infected parent virus. 2) Lymphomagenic viruses consisted of plural viral subpopulations. Recombinant mink cell focus-inducing (MCF) and ecotropic viruses were cloned from them. Inoculation of either MCF or ecotropic virus alone or both viruses together did not cause lymphoma in NFS/N mice and there was no evidence of viral replication in the recipients. 3) Inoculation of either MCF- or ecotropic virus-infected NFS-ME cells alone did not cause lymphoma development in pre-irradiated NFS/N mice, while transplantation of both MCF- and ecotropic virus-infected NFS-ME cells resulted in the development of lymphomas of host origin. These results show that lymphomagenic MCF virus was generated through the recombination of E4 viral genome and a modified proviral DNA of endogenous viruses present in radiation- or chemically-induced lymphomas, and that an interaction or synergism of MCF and ecotropic viruses is required for MCF virus to exert lymphomagenic activity.
authors.forename: K
authors.initials: K
authors.lastname: Yanagihara
authors.forename: T
authors.initials: T
authors.lastname: Seyama
authors.forename: K
authors.initials: K
authors.lastname: Kamiya
authors.forename: A
authors.initials: A
authors.lastname: Inoh
authors.forename: K
authors.initials: K
authors.lastname: Yokoro
mesh_terms: D000818:Animals; D002460:Cell Line; D005038:Ethylnitrosourea; D005260:Female; D005347:Fibroblasts / Q000382:microbiology; D008223:Lymphoma / Q000139:chemically induced / Q000382:microbiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008907:Mink; D009381:Neoplasms, Radiation-Induced / Q000382:microbiology*; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D012190:Retroviridae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D013045:Species Specificity; D013601:T-Lymphocytes; D014412:Tumor Virus Infections / Q000382:microbiology*
chemical_list: D005038:Ethylnitrosourea