Identification of immediate early genes from herpes simplex virus that transactivate the virus thymidine kinase gene.
A HeLa cell transient-expression assay system was used to determine if isolated immediate early (alpha) genes from herpes simplex virus (HSV) could transcriptionally activate (transactivate) the type 1 (HSV-1) thymidine kinase (TK) gene [an early (beta) gene]. Cells transfected with the TK gene alone transcribed very low levels of TK RNA. Cells cotransfected with plasmids bearing the sequences that encode the alpha-gene product infected cell protein 0 or 4 (ICP0 or ICP4) and the TK gene faithfully transcribed high levels of TK RNA. The plasmid containing the sequences encoding ICP0 was a more potent transactivator than the plasmid containing the sequences for ICP4.
Gelman
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Silverstein
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D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D012333:RNA, Messenger; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes