Biologically active proviral clone of myeloblastosis-associated virus type 1: implications for the genesis of avian myeloblastosis virus.
A biologically active myeloblastosis-associated virus (MAV) provirus was cloned from a bacteriophage recombinant library constructed from leukemic chicken myeloblast DNA. The restriction endonuclease map of this clone was consistent with that of a type 1 MAV (MAV-1). Interference assays of virus recovered from cultured chicken embryo fibroblasts after DNA transfection established that the provirus was infectious and confirmed that it belonged to avian retrovirus subgroup A (type 1). Antipeptide antibodies raised against the env-encoded carboxyl terminus of p48myb, the transforming protein of avian myeloblastosis virus, specifically immunoprecipitated the gp37env from quail cells transfected with MAV-1 proviral DNA but not from cells infected with MAV-2. This suggests that MAV-1 rather than MAV-2 is the progenitor helper virus from which avian myeloblastosis virus arose by the transduction of cellular proto-oncogene sequences.
Perbal
B
B
Lipsick
J S
JS
Svoboda
J
J
Silva
R F
RF
Baluda
M A
MA
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D002645:Chickens; D003001:Cloning, Molecular; D003429:Cross Reactions; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D006378:Helper Viruses / Q000235:genetics* / Q000276:immunology; D014162:Transfection; D014759:Viral Envelope Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology; D014779:Virus Replication
D004279:DNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D004262:DNA Restriction Enzymes