Studies on four cellular proto-oncogenes and their expression in PCC4 embryonal carcinoma cells: amplification of c-Ki-ras oncogene.
We examined the expression of four cellular proto-oncogenes in two teratocarcinoma cell lines, one undifferentiated and malignant (PCC4), one differentiated and non malignant (PCD1). In this paper we report that transcript levels of c-Ki-ras, c-myc and c-fos are significantly higher in PCC4 cells whereas transcripts of c-Ha-ras are unchanged. Southern blot analysis does not reveal any structural alteration of c-myc and c-fos in PCC4 cells. On the contrary, the c-Ki-ras proto-oncogene is amplified 10 to 20 fold in PCC4 cells.
Vilette
D
D
Emanoil-Ravier
R
R
Tobaly
J
J
Peries
J
J
D000818:Animals; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D005786:Gene Expression Regulation; D051379:Mice; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D013724:Teratoma / Q000235:genetics*; D014158:Transcription, Genetic
D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII