Chromosomal site of hepatitis B virus (HBV) integration in a human hepatocellular carcinoma-derived cell line.
The single site of integration of hepatitis B virus in the human hepatocellular carcinoma cell line Hep 3B 2-1/7 was found to segregate with human chromosome 12 in somatic cell hybrids. Analysis of metaphase spreads of Hep 3B 2-1/7 following in situ hybridization with pHBV revealed integration at 12q13----q14, a location that coincides with a fragile site, fra (12q13). The possible significance of this location to the development of hepatocellular carcinomas is discussed.
Simon
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D
Searls
D B
DB
Cao
Y
Y
Sun
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K
Knowles
B B
BB
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000382:microbiology; D002460:Cell Line; D002906:Chromosomes, Human, 6-12 and X; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics* / Q000382:microbiology; D008114:Liver Neoplasms, Experimental / Q000235:genetics; D051381:Rats
D006514:Hepatitis B Surface Antigens