title: Modulation of the sodium periodate (NaIO4) response in systemic lupus erythematosus (SLE): effect of interleukin-1 (IL-1), interleukin-2 (IL-2), phorbol myristate acetate (PMA), and indomethacin.
abstract: The proliferative activity of peripheral blood mononuclear cells (PBMN) from patients with systemic lupus erythematosus (SLE) activated by sodium periodate (NaIO4) is greatly diminished. The effect of the cytokines Interleukin-1 (IL-1) and Interleukin-2 (IL-2) on the NaIO4 reaction was investigated. Addition of IL-1 resulted in partial restoration of the reaction of SLE PBMN to NaIO4, and a similar effect was demonstrated in the presence of phorbol myristate acetate (PMA). Addition of IL-2 to NaIO4 activated SLE PBMN, however, caused a marked improvement in their proliferative activity. The presence of indomethacin resulted in only a slight increase in [3H]thymidine incorporation by the NaIO4-treated SLE cells. The results suggest that the defect in the response of SLE PBMN cells to NaIO4 is due to inadequate availability of IL-1 and IL-2. Excessive production of prostaglandin in SLE might also contribute to the defective response to NaIO4 but does not appear to play a major role.
authors.forename: R
authors.initials: R
authors.lastname: Phillips
authors.forename: R
authors.initials: R
authors.lastname: Lomnitzer
authors.forename: A R
authors.initials: AR
authors.lastname: Rabson
mesh_terms: D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000503:physiopathology*; D008297:Male; D008934:Mitogens / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects; D010504:Periodic Acid / Q000502:physiology*; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D007375:Interleukin-1; D007376:Interleukin-2; D008934:Mitogens; D010704:Phorbols; D010504:Periodic Acid; C009288:metaperiodate; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin