Adult T cell leukemia cell lines that originated from primary leukemic clones also had a defect of expression of CD3-T cell receptor complex.
Surface phenotypes and genotypes of six T cell lines established from four patients with adult T cell leukemia (ATL) were compared with those of fresh ATL cells. The surface antigen densities of CD3 and T cell receptor (TCR) complex examined by OKT3 and WT-31 mAbs on fresh ATL cells were low. But three IL-2-dependent cell lines, SKT-2, SKT-5, and ATL-17-2, established from three patients expressed a high density of CD3-TCR complex on their surfaces. On the contrary, three other cell lines (SKT-1A, SKT-1B, and MMT-2), established from two other patients, expressed a low density of CD3-TCR complex. Genotypic analysis of TCR beta chain (T beta) gene and integration sites of the proviral genome of human T cell lymphotropic virus type 1 demonstrated that SKT-1A, SKT-1B, and MMT-2 were derived from the original leukemic clones. These apparent associations between a defect of expression of CD3-TCR complex and identical genotypes of fresh and cultured cells suggest that the defect of expression of CD3-TCR complex may play a key role for development of ATL.
Shirono
K
K
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
Hattori
T
T
Matsuoka
M
M
Matsushita
S
S
Asou
N
N
Takatsuki
K
K
D000328:Adult; D000368:Aged; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000032:analysis; D017252:CD3 Complex; D002460:Cell Line; D002474:Cell-Free System; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005838:Genotype; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis / Q000502:physiology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000302:isolation & purification*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
D000945:Antigens, Differentiation, T-Lymphocyte; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell