Gluconate kinase from Zymomonas mobilis: isolation and characteristics.
The enzyme gluconate kinase EC 2.7.1.12 has been found at high levels in glucose-grown Zymomonas mobilis cells. A simple procedure, based on differential dye-ligand chromatography, has been used to isolate the enzyme, purifying it some 600-fold. The purified enzyme is a monomer of molecular weight 18,000 Da, which is much smaller than other gluconate kinases reported. It has a relatively low affinity for ATP. (Km = 1.5 mM), but high for gluconate (Km = 0.33 mM), and has little activity with any other potential substrates.
Zachariou
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D002845:Chromatography; D007700:Kinetics; D010770:Phosphotransferases / Q000302:isolation & purification* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011548:Pseudomonadaceae / Q000201:enzymology*
D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020578:gluconokinase