A role for L3T4+ T cells and their lymphokines in the generation of suppressor effector (TS3) cells.
The cellular requirements for the in vitro induction of antigen-specific suppressor T cells were examined. Previous reports indicated that Ia-bearing macrophages and anti-idiotypic B cells are required as accessory cells to facilitate the generation of suppressor effector (TS3) cells which regulate the response to the 4-hydroxy-3-nitrophenyl acetyl (NP) hapten. The present study describes two distinct T cell populations which interact to generate antigen-specific TS3. Fractionation of the T cell populations with monoclonal antibody to the L3T4 determinant led to the identification of an NP-specific L3T4- TS3 progenitor population and an L3T4+ helper/inducer subset. In the presence of NP-coupled antigen, the L3T4+ subset could induce progenitor TS3 to differentiate into mature TS3 cells. The activity of the L3T4+ inducer population could be replaced with specifically activated cloned helper cells which were not NP-reactive since an I-Ab-restricted, insulin-reactive, L3T4+ clone was capable of supporting the generation of NP-specific TS3. Inducer activity appeared to be confined to the Th1 but not the Th2 subset. In addition, 18-hr supernatants from antigen-activated clones were capable of substituting for L3T4+ cells or T cell clones in TS3 induction cultures. The TS maturation/differentiation factor(s) active in these supernatants does not appear to be IL-1, IL-2, IL-3, or interferon-gamma alone since purified sources of these lymphokines failed to induce TS3 activity.
O'Hara
R M
RM
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Hausman
P B
PB
Sherr
D H
DH
Abromson-Leeman
S R
SR
Dorf
M E
ME
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D001685:Biological Factors / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells; D007700:Kinetics; D008222:Lymphokines / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*
D000945:Antigens, Differentiation, T-Lymphocyte; D001685:Biological Factors; D008222:Lymphokines