The HLA-AW24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes.
A cosmid clone containing two class I sequences was found to cause expression of the HLA-AW24 protein after transfection into mouse L cells. The restriction map of this cosmid shows extensive homology over 26 kb with the map of the HLA-A3 region obtained from cosmids of the same library, constructed with DNA from an HLA-A3/HLA-AW24 heterozygote, but diverges over the remaining 14 kb. The HLA-AW24 gene was subcloned from this cosmid and its nucleotide sequence was determined. Amino acid and, more strikingly, nucleotide sequence comparisons with other HLA alleles indicate that the A locus alleles are more closely related to each other than to alleles from other HLA loci. A very skewed distribution of silent substitutions is apparent, and the occurrence of clustered multiple substitutions hints at gene-conversion-like events.
N'Guyen
C
C
Sodoyer
R
R
Trucy
J
J
Strachan
T
T
Jordan
B R
BR
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; D059871:HLA-A24 Antigen; D015791:HLA-A3 Antigen; D006801:Humans; D007739:L Cells / Q000276:immunology; D051379:Mice; D014162:Transfection
D006680:HLA Antigens; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; D059871:HLA-A24 Antigen; D015791:HLA-A3 Antigen; D004262:DNA Restriction Enzymes