title: A role for glycosylation of the alpha subunit in transduction of biological signal in glycoprotein hormones.
abstract: The biological properties of recombinants of glycoprotein hormones in which the alpha and beta subunits were differentially deglycosylated have been investigated. Specific deglycosylation of the alpha subunit generated a recombinant that had more receptor-binding activity but did not produce hormone response in the target cells. The deglycosylated alpha + beta recombinant was also an antagonist of the action of the native hormone. Thus, the carbohydrates in the alpha subunit play a dominant role in the transduction of the hormone signal into the cell.
authors.forename: M R
authors.initials: MR
authors.lastname: Sairam
authors.forename: G N
authors.initials: GN
authors.lastname: Bhargavi
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology*; D006023:Glycoproteins / Q000502:physiology*; D007985:Leydig Cells / Q000502:physiology; D007986:Luteinizing Hormone / Q000502:physiology*; D008297:Male; D010053:Ovary / Q000502:physiology; D051381:Rats; D011956:Receptors, Cell Surface / Q000502:physiology*; D011962:Receptors, FSH; D011974:Receptors, LH; D012671:Seminiferous Tubules / Q000502:physiology; D012756:Sheep; D013329:Structure-Activity Relationship; D013552:Swine
chemical_list: D006023:Glycoproteins; D011956:Receptors, Cell Surface; D011962:Receptors, FSH; D011974:Receptors, LH; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP