The expression of ceruloplasmin, an angiogenic glycoprotein, by mouse embryonic fibroblasts.
Balb/c 3T3, Swiss 3T3 and Rous sarcoma virus transformed Balb/c 3T3 mouse embryonic fibroblasts produced ceruloplasmin in vitro, whereas primary cultures prepared from the Balb/c mouse embryos did not produce ceruloplasmin. The amount of ceruloplasmin synthesis by the Balb/c 3T3 cell line is enhanced by Rous sarcoma virus-transformation (1.5-3 fold) and by treatment with dexamethasone (about 2.4 fold). The protein was identified as ceruloplasmin by immunoprecipitation with ceruloplasmin-specific polyclonal antibody, and by similarity of peptide maps, and subunit molecular weight (135,000 dalton) to that of authentic ceruloplasmin from primary cultures of mouse hepatocytes.
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D000818:Animals; D001358:Avian Sarcoma Viruses; D002472:Cell Transformation, Viral / Q000187:drug effects; D002478:Cells, Cultured; D002570:Ceruloplasmin / Q000096:biosynthesis*; D006224:Cricetinae; D003907:Dexamethasone / Q000494:pharmacology; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011247:Pregnancy; D051381:Rats
D005353:Fibronectins; D003907:Dexamethasone; D002570:Ceruloplasmin