Cell cycle position and expression of encephalomyocarditis virus in mouse embryo fibroblasts.
Infection of mouse embryo fibroblasts in G1 or S phase with encephalomyocarditis virus gave different kinetics of viral RNA synthesis. In S phase cells, RNA synthesis was faster and reached higher levels than in G1 cells. Virus-specified proteins were fewer in G1 cells than in S cells during the early stage of the infection and c.p.e. in G1 cells appeared about 4 h later than in S cells. Addition of a cellular factor with ability to affect cell conformation had an inhibitory effect on viral RNA synthesis.
Mallucci
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L
Wells
V
V
Beare
D
D
D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004622:Embryo, Mammalian; D004686:Encephalomyocarditis virus / Q000378:metabolism* / Q000502:physiology; D005347:Fibroblasts; D007399:Interphase; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000494:pharmacology; D012367:RNA, Viral / Q000096:biosynthesis*; D014764:Viral Proteins / Q000096:biosynthesis*
D011506:Proteins; D012367:RNA, Viral; D014764:Viral Proteins