title: Identification of cross-reacting glycoproteins of four herpesviruses by Western blotting.
abstract: Monospecific rabbit antisera against purified herpes simplex virus type 1 (HSV-1) gB, gC or gD antigens or polyvalent rabbit antiserum against equine herpesvirus type 1 (EHV-1)-infected cells was used in Western blotting to identify antigenically related proteins in cells infected with HSV-1, HSV-2, bovine mammillitis virus or EHV-1. Monomeric and oligomeric polypeptides related to HSV-1 gB were found in cells infected with each of the four herpesviruses. The gC antiserum was specific for HSV-1 and the gD antiserum reacted only with polypeptides in HSV-1- or HSV-2-infected cells. Neither gC nor gD antiserum revealed the existence of oligomeric forms of the glycoproteins with the single exception of HSV-1 strain KOS which had a heat-unstable high molecular weight form gD. The EHV-1 antiserum reacted with high molecular weight polypeptides of the same mobility as the oligomeric form of gB in cells infected with each of the four herpesviruses.
authors.forename: B W
authors.initials: BW
authors.lastname: Snowden
authors.forename: I W
authors.initials: IW
authors.lastname: Halliburton
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D006224:Cricetinae; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D006564:Herpesviridae / Q000276:immunology*; D001911:Herpesvirus 2, Bovine / Q000276:immunology; D006736:Horses; D007668:Kidney; D051381:Rats; D018139:Simplexvirus / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins