Two electropherotypes of bluetongue virus serotype 2 from naturally infected calves.
The first isolation of bluetongue virus (BTV) serotype 2 in the U.S.A. was in 1982 from a sentinel herd of cattle at Ona, Florida. Electrophoretic analysis of genome RNA revealed that all 16 serotype 2 isolates obtained from this focus of infection had one of two electropherotypes (designated Ona A and Ona B). All genome segments of Ona A and Ona B had detectable differences in electrophoretic mobility, with major differences noted for segments 1, 4, 5, 7, 8, 9 and 10. Electrophoretic comparison revealed that Ona A was indistinguishable from the African serotype 2 prototype isolated 23 years earlier. In 1983, Ona B, in the apparent absence of Ona A, was isolated from two additional cattle herds in Florida. These results suggest that Ona B may be a variant of Ona A that evolved as a result of mutation or reassortment with another BTV strain, and may be better adapted to the selective pressures found in southern Florida. Comparison of the electropherotypes of Ona B with two Florida isolates of serotype 13 and 17 and the prototypes of BTV 10, 11, 13 or 17 produced no evidence for reassortment between Ona A and any of these strains as the possible origin of Ona B.
Collisson
E W
EW
Barber
T L
TL
Gibbs
E P
EP
Greiner
E C
EC
D000349:Africa; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001819:Bluetongue / Q000382:microbiology; D001820:Bluetongue virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005431:Florida; D005814:Genes, Viral; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000145:classification*; D012703:Serotyping; D012756:Sheep; D014764:Viral Proteins / Q000032:analysis
D000956:Antigens, Viral; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins