title: Nucleotide sequence of the Bacillus subtilis xylose isomerase gene: extensive homology between the Bacillus and Escherichia coli enzyme.
abstract: The xylose isomerase gene from Bacillus subtilis was cloned from a genomic BamH1 library by complementation of an isomerase defective Escherichia coli strain as previously described. The ATG initiation codon is preceded by a Shine-Dalgarno sequence and two hexamers being characteristic for the promoter region of Bacillus genes. The structural gene consists of 1320 base pairs, thus coding for a polypeptide chain of 440 amino acids with a molecular weight of 49 680. The polypeptide primary structure shows over 50% homology to that of the E. coli xylose isomerase.
authors.forename: M
authors.initials: M
authors.lastname: Wilhelm
authors.forename: C P
authors.initials: CP
authors.lastname: Hollenberg
mesh_terms: D019747:Aldose-Ketose Isomerases; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002238:Carbohydrate Epimerases / Q000235:genetics*; D003062:Codon; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D009876:Operon; D010770:Phosphotransferases / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor)
chemical_list: D003062:Codon; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase