Isolation, characterization, and regulation of the prolactin receptor.
The prolactin, or lactogenic hormone, receptor has been purified (approximately 80%) from lactating mouse liver and human term placenta by the nondenaturing zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate and a prolactin affinity column. The isolated "core-binding unit" has a molecular weight of 37,000 +/- 2,000 daltons. It retains the specificity for lactogenic hormones and binds prolactin with an affinity (Ka = 2 to 6 X 10(9) M-1) similar to that of the receptor as it occurs in its membranous environment (Ka = 3 to 5 X 10(9) M-1). Whether this "core-binding unit" exists on the cell surface in a cryptic or active form is influenced greatly by its association with other membrane proteins and the concentration of phosphatidylcholine within its local membranous environment.
Vonderhaar
B K
BK
Bhattacharya
A
A
Alhadi
T
T
Liscia
D S
DS
Andrew
E M
EM
Young
J K
JK
Ginsburg
E
E
Bhattacharjee
M
M
Horn
T M
TM
D000818:Animals; D002793:Cholic Acids; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D008099:Liver / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008861:Microsomes / Q000032:analysis; D008862:Microsomes, Liver / Q000032:analysis; D008954:Models, Biological; D008970:Molecular Weight; D010743:Phospholipids / Q000378:metabolism; D010920:Placenta / Q000032:analysis; D011388:Prolactin / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism; D011981:Receptors, Prolactin; D012995:Solubility
D002793:Cholic Acids; D010743:Phospholipids; D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D003208:Concanavalin A; D011388:Prolactin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate