The influence of ipratropium bromide and sodium cromoglycate on benzalkonium chloride-induced bronchoconstriction in asthma.
1. Benzalkonium chloride, an antibacterial preservative that is added to nebuliser solutions, has been shown to cause bronchoconstriction when inhaled by asthmatic subjects. 2. To investigate the potential role of reflex and mast cell-dependent mechanisms in the pathogenesis of bronchoconstriction produced by benzalkonium chloride we examined the effects of ipratropium bromide and sodium cromoglycate on this response in both concentration-response and time-course studies in nine asthmatic subjects. 3. Pretreatment with inhaled ipratropium bromide (1 mg) and sodium cromoglycate (40 mg) displaced the benzalkonium chloride concentration-response curves to the right by a mean 2.2 fold and 3.1 fold respectively. 4. Ipratropium bromide and sodium cromoglycate markedly attenuated the airway response to benzalkonium chloride throughout the 45 min time course period, inhibiting the overall response by 56% and 78% respectively. 5. We conclude that benzalkonium chloride provokes bronchoconstriction in asthmatic subjects through a combination of mast cell activation and stimulation of peripheral and central neural pathways.
Miszkiel
K A
KA
Immunopharmacology Group, Southampton General Hospital, Shirley.
Beasley
R
R
Holgate
S T
ST
D000328:Adult; D001249:Asthma / Q000503:physiopathology*; D001286:Atropine Derivatives / Q000494:pharmacology*; D001548:Benzalkonium Compounds / Q000494:pharmacology*; D001980:Bronchi / Q000187:drug effects* / Q000503:physiopathology; D004205:Cromolyn Sodium / Q000494:pharmacology*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D009241:Ipratropium / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged
D001286:Atropine Derivatives; D001548:Benzalkonium Compounds; D009241:Ipratropium; D004205:Cromolyn Sodium