title: Kinetics of IgM and IgG responses to Japanese encephalitis virus in human serum and cerebrospinal fluid.
abstract: We measured levels of antibodies to Japanese encephalitis virus (JEV) in serum and in cerebrospinal fluid (CSF) specimens obtained from 32 patients with acute encephalitis by using "antibody-capture" solid-phase enzyme-linked immunoassays specific for IgM or IgG to JEV. The proportions of confirmed cases with IgM to JEV detectable in CSF were 68% (obtained on day 1), 100% (day 7), 96% (day 30), and 72% (day 180). For IgG in CSF the proportions were 47% (day 1), 89% (day 7), 100% (day 30), and 100% (day 180). Twenty-five CSF samples were obtained from control patients with other diseases with possible nervous system involvement (but none with a clinical diagnosis of viral encephalitis); none had detectable IgM to JEV. Five JEV-infected but asymptomatic siblings of patients with encephalitis were also examined; all had high levels of IgM to JEV in serum, but none had detectable IgM to JEV in CSF.
authors.forename: D S
authors.initials: DS
authors.lastname: Burke
authors.forename: A
authors.initials: A
authors.lastname: Nisalak
authors.forename: M A
authors.initials: MA
authors.lastname: Ussery
authors.forename: T
authors.initials: T
authors.lastname: Laorakpongse
authors.forename: S
authors.initials: S
authors.lastname: Chantavibul
mesh_terms: D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000134:cerebrospinal fluid; D002555:Cerebrospinal Fluid / Q000166:cytology; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000134:cerebrospinal fluid / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007958:Leukocyte Count; D008657:Metabolic Clearance Rate; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M