Protection and deprotection of horse cytochrome c.
The last step in the semisynthesis of horse cytochrome c analogues (formation of the bond 65-66) requires the conformation of the complex between two complementary fragments, (1-65) lactone and (66-104). The fragments can be obtained from a limited degradation with cyanogen bromide. The amino component in this reaction can also be obtained from organo chemical synthesis in which the C-terminal fragment (81-104) is required in a selectively protected form. The latter is available from a cyanogen bromide degradation of ubiquitously protected cytochrome c. The details of the protection/deprotection reaction and the properties of nonadecamethylsulfonylethyloxycarbonyl cytochrome c are described.
Boon
P J
PJ
Tesser
G I
GI
D000595:Amino Acid Sequence; D000818:Animals; D003488:Cyanogen Bromide; D003574:Cytochrome c Group / Q000138:chemical synthesis* / Q000378:metabolism; D006736:Horses; D007202:Indicators and Reagents; D009206:Myocardium; D010446:Peptide Fragments; D010455:Peptides / Q000138:chemical synthesis
D003574:Cytochrome c Group; D007202:Indicators and Reagents; D010446:Peptide Fragments; D010455:Peptides; D003488:Cyanogen Bromide