Effects of food deprivation and high fat diet on immunoreactive beta-endorphin levels in brain regions of Zucker rats.
The levels of immunoreactive beta-endorphin (ir-beta-EP) were measured in the brain and pituitary of lean Zucker rats subjected to food deprivation for 72 h and to a high fat diet, and in fatty Zucker rats after food deprivation for 72 h. Ir-beta-EP was increased in the neurointermediate (NI-) pituitary lobe but reduced in the medulla-pons of fatty rats when compared to lean littermates fed ad libitum. Food deprivation decreased ir-beta-EP in the cortex and medulla-pons of lean rats and in the cortex, midbrain and NI-pituitary of fatty rats. In contrast, ir-beta-EP was increased in the anterior pituitary of lean rats and in the striatum of fatty rats after deprivation. The high fat diet produced a decrease in ir-beta-EP in the cortex, midbrain and NI-pituitary with an increase in the striatum and hypothalamus of lean rats. These results suggest that the ir-beta-EP concentration could be differentially affected in different brain regions of Zucker rats by changes in the energy balance.
Tsujii
S
S
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Nakai
Y
Y
Fukata
J
J
Nakaishi
S
S
Takahashi
H
H
Usui
T
T
Imura
H
H
D000818:Animals; D001921:Brain / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D001923:Brain Chemistry; D004041:Dietary Fats / Q000494:pharmacology*; D005508:Food Deprivation / Q000502:physiology*; D008297:Male; D010902:Pituitary Gland / Q000032:analysis / Q000276:immunology / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D011924:Rats, Zucker; D001615:beta-Endorphin / Q000032:analysis / Q000276:immunology
D004041:Dietary Fats; D001615:beta-Endorphin