Three-dimensional collagen matrices as cell culture substrata affect the generation of alloreactive cytotoxic T lymphocytes.
Primary one-way mixed lymphocyte cultures (MLC) of C3H/He responder and DBA/2 stimulator were performed in three-dimensional (3-D) collagen matrices and the generation of alloreactive cytotoxic T cell (CTL) responses was compared to those in MLC which were done on usual plastic surfaces or on collagen-coated plastic surfaces. MLC in the 3-D collagen matrices were found to generate strong CTL responses. Flow cytometric analysis of Lyt-2 and L3T4 antigen expressions on the effector cells showed that the Lyt-2/L3T4 ratios were substantially higher in the 3-D collagen matrices, and that a larger proportion of the cells in the 3-D collagen matrices were Lyt-2+ lymphoblasts. These results indicate that the milieu of the 3-D collagen matrices favors the proliferation of Lyt-2+ lymphocytes, and suggests that cell-to-matrix interactions in 3-D collagen matrices may play a regulatory role in the maturation process of alloreactive CTLs.
Kubota
K
K
Department of Microbiology, Kitasato University School of Medicine, Kanagawa, Japan.
Tamauchi
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D003094:Collagen; D005434:Flow Cytometry; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D051379:Mice; D008815:Mice, Inbred Strains; D010969:Plastics; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D010969:Plastics; D003094:Collagen