A study of enterotoxigenic Escherichia coli, serogroup 0126, by bacterial restriction endonuclease DNA analysis (BRENDA).
Sixteen isolates of Escherichia coli were subjected to bacterial restriction endonuclease DNA analysis (BRENDA). Nine of these isolates were from an outbreak of human diarrhoea and produced stable toxin, the remaining seven were non-toxigenic strains from animal and human sources. The isolates from the outbreak produced indistinguishable DNA electrophoretic patterns in spite of their assignment to seven different H serotypes. Their BRENDA patterns were markedly different from the other isolates examined. These results support the epidemiological evidence that a single-strain outbreak had occurred, and they cast doubt on the value of H typing for this particular investigation.
Marshall
R B
RB
Winter
P J
PJ
Robinson
A J
AJ
Bettelheim
K A
KA
D001427:Bacterial Toxins; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI; D004196:Disease Outbreaks; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000032:analysis / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D029968:Escherichia coli Proteins; D005759:Gastroenteritis / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D012703:Serotyping
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI