Comparison of neutralization and DNA restriction enzyme methods for typing clinical isolates of human adenovirus.
Sixty-five adenovirus isolates collected over a 3.5-year period were typed by both standard microneutralization techniques and restriction endonuclease digestion of viral DNA. Of the 65 isolates, 47 (72.3%) representing six adenovirus types could be typed by microneutralization. Eighteen isolates demonstrated partial neutralization with standard antisera to two or more adenovirus serotypes and thus could not be definitively typed. DNA analysis permitted typing of 64 of the 65 isolates (98.5%) (including four isolates which contained mixtures of two adenovirus types), and 12 different types were identified. Neutralization and DNA typing disagreed for five isolates, and in each case, digestion with multiple restriction endonucleases and DNA hybridization studies were consistent with the type assigned by DNA analysis. In addition, the DNA analysis method allowed the identification of genomic variants (genome types) of five adenovirus types. We conclude that typing clinical isolates of adenovirus by restriction endonuclease digestion of viral DNA can be done rapidly, provides additional epidemiological and typing information, and provides fewer ambiguous results than does typing by neutralization.
Fife
K H
KH
Ashley
R
R
Shields
A F
AF
Salter
D
D
Meyers
J D
JD
Corey
L
L
D000260:Adenoviruses, Human / Q000145:classification* / Q000201:enzymology / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D002500:Centrifugation, Isopycnic; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis* / Q000276:immunology; D004586:Electrophoresis; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D006801:Humans; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral; D004262:DNA Restriction Enzymes