title: Detection of human polyomavirus DNA in urine specimens by hybridot assay.
abstract: A hybridot assay method using a labelled BK virus probe has been used to detect the presence of human polyomavirus DNA in 81 urine specimens from 61 patients, most of whom were immuno-compromised to some degree. Twenty-eight urines from 23 patients had detectable DNA. The results have been compared to virus isolation and electron microscopy on the same specimens. In 90 per cent a comparable result was obtained by at least one of the other two tests and in the 73 specimens on which all 3 tests were performed there was 80 per cent agreement between all. In 6 cases the hybridot assay was more sensitive in detecting infection.
authors.forename: P E
authors.initials: PE
authors.lastname: Gibson
authors.forename: S D
authors.initials: SD
authors.lastname: Gardner
authors.forename: A A
authors.initials: AA
authors.lastname: Porter
mesh_terms: D000818:Animals; D001739:BK Virus / Q000302:isolation & purification; D004279:DNA, Viral / Q000652:urine*; D006801:Humans; D007577:JC Virus / Q000302:isolation & purification; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000302:isolation & purification; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine; D014556:Urine / Q000382:microbiology*
chemical_list: D004279:DNA, Viral