Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes.
We have examined the distribution of human papillomavirus (HPV) DNA in paraffin sections of humans warts by in situ hybridization with biotin-labeled DNA probes. Recombinant plasmid DNAs (HPV-1, -6, -11, -16) were labeled by nick translation with biotinylated deoxyuridine triphosphate. Paraffin sections were hybridized with the probes for 18 h in stringent or non-stringent conditions, and DNA-DNA hybrids were detected by immunocytochemistry. Paraffin sections of warts were also examined for the presence of HPV capsid antigen with the avidin-biotin peroxidase complex method for immunocytochemistry. HPV DNA was detected and localized in paraffin sections from a plantar wart, a laryngeal papilloma, and seven anogenital condylomas. The specific HPV type present in each lesion was determined by hybridization under stringent conditions with the homologous DNA probe. The papillomas were found to contain many more cells with replicating virus DNA, as demonstrated by in situ hybridization, than was apparent from the number of cells containing detectable virus antigen. In situ hybridization with biotin-labeled probes is an effective technique for the identification of HPV infection in routinely collected and processed tissue specimens.
Beckmann
A M
AM
Myerson
D
D
Daling
J R
JR
Kiviat
N B
NB
Fenoglio
C M
CM
McDougall
J K
JK
D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001005:Anus Neoplasms / Q000032:analysis / Q000382:microbiology; D001710:Biotin; D003218:Condylomata Acuminata / Q000032:analysis* / Q000382:microbiology; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005833:Genital Neoplasms, Female / Q000032:analysis / Q000382:microbiology; D005834:Genital Neoplasms, Male / Q000032:analysis / Q000382:microbiology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000032:analysis / Q000382:microbiology; D008297:Male; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000032:analysis / Q000382:microbiology; D027383:Papillomaviridae / Q000032:analysis* / Q000276:immunology; D014860:Warts / Q000235:genetics / Q000276:immunology / Q000382:microbiology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral; D001710:Biotin