title: Localization by in situ hybridization of the coagulation factor IX gene and of two polymorphic DNA probes with respect to the fragile X site.
abstract: The coagulation factor IX gene and two other polymorphic loci corresponding to DNA probes 52 A and St 14 have been previously localized in the q27 to qter region of the human X chromosome. In order to study their localization with respect to the fragile site at Xq27-28, we have hybridized the three DNA probes to metaphase chromosomes of a boy with fragile X mental retardation. We show that probe 52 A is located in the proximal part of the Xq27 band, while the coagulation factor IX gene is on the distal part of this band, but proximal to the fragile site. The very polymorphic St 14 probe is located in the distal part of the Xq28 band, on the other side of the fragile site.
authors.forename: M G
authors.initials: MG
authors.lastname: Mattei
authors.forename: M A
authors.initials: MA
authors.lastname: Baeteman
authors.forename: R
authors.initials: R
authors.lastname: Heilig
authors.forename: I
authors.initials: I
authors.lastname: Oberlé
authors.forename: K
authors.initials: K
authors.lastname: Davies
authors.forename: J L
authors.initials: JL
authors.lastname: Mandel
authors.forename: J F
authors.initials: JF
authors.lastname: Mattei
mesh_terms: D002871:Chromosome Banding; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005164:Factor IX / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D014960:X Chromosome
chemical_list: D005819:Genetic Markers; D005164:Factor IX; D004247:DNA; D004262:DNA Restriction Enzymes