title: Antibodies to calcium/phospholipid binding protein (calelectrin) recognize neurons, astrocytes and Schwann cells in the nervous system of rat.
abstract: We report on the identification of proteins Mr 32 kDa, 34 kDa, 68 kDa in rat brain or sciatic nerve which cross-react (by immunoblotting) with antibodies to purified Ca2+/phospholipid binding protein calelectrin from Torpedo marmorata. Immunocytochemical staining revealed that anti-calelectrin antibodies labeled cell bodies and processes of cortical neurons and astrocytes in the central nervous system (CNS). In sciatic nerve, calelectrin-like immunoreactivity was expressed in the cytoplasm of Schwann cells of adult as well as newborn rat. Additional staining occurred at periaxonal sites in mature nerve. The localization of calelectrin-related proteins in distinct cell types of the mammalian nervous system suggests a cell-specific role of this new group of proteins in Ca2+-mediated membrane processes involved in neural function.
authors.affiliation: Labor für Molekulare Neurobiologie, Universität Düsseldorf, F.R.G.
authors.forename: G
authors.initials: G
authors.lastname: Stoll
authors.forename: U
authors.initials: U
authors.lastname: Fritsche
authors.forename: V
authors.initials: V
authors.lastname: Witzemann
authors.forename: H W
authors.initials: HW
authors.lastname: Müller
mesh_terms: D000818:Animals; D017302:Annexins; D000906:Antibodies / Q000378:metabolism; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000276:immunology* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000378:metabolism*; D014101:Torpedo
chemical_list: D017302:Annexins; D000906:Antibodies; D002135:Calcium-Binding Proteins