Characterization of the vasopressin receptor on WRK-1 cells.
Specific vasopressin binding to WRK-1 rat mammary tumor cells was assessed and compared with vasopressin-induced alterations in phosphatidylinositol metabolism. Scatchard analysis revealed the presence of two binding sites: a saturable, high affinity site with a dissociation constant of 1 X 10(-9) M and an n of 2700 sites per cell, and a nonsaturable, apparent lower affinity site. The higher affinity site appeared to have V1a specificity and to correlate with vasopressin's ability to stimulate phosphatidylinositol turnover in the cells.
Woods
D J
DJ
Department of Physiology and Biophysics, New York University Medical Center, New York 10016.
Monaco
M E
ME
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D011945:Receptors, Angiotensin / Q000378:metabolism*; D017483:Receptors, Vasopressin; D014407:Tumor Cells, Cultured; D014667:Vasopressins / Q000494:pharmacology
D010716:Phosphatidylinositols; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D014667:Vasopressins; D001127:Arginine Vasopressin