title: Amiloride-sensitive Na+-H+ antiporter in Escherichia coli.
abstract: In everted vesicles of Escherichia coli, delta pH caused by H+ efflux through the Na+/H+ antiporter was measured by using a fluorescent dye. Amiloride inhibited the activity of the Na+/H+ antiporter. Kinetic studies showed that amiloride competed with Na+. The inhibition constant of 40 microM was obtained.
authors.forename: N
authors.initials: N
authors.lastname: Mochizuki-Oda
authors.forename: F
authors.initials: F
authors.lastname: Oosawa
mesh_terms: D000584:Amiloride / Q000494:pharmacology*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D011719:Pyrazines / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers; D013050:Spectrometry, Fluorescence
chemical_list: D002352:Carrier Proteins; D011719:Pyrazines; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride