Specific transforming potential of oncogenes encoding protein-tyrosine kinases.
Several chimeric murine retroviruses were constructed to test whether the gag sequence of Abelson murine leukemia virus (A-MuLV) could influence the in vitro specificity of two sarcoma-inducing oncogenes: src of Rous sarcoma virus and fps of Fujinami sarcoma virus. Although the src- or fps- containing chimerae could transform fibroblasts, they were unable to mimic the action of A-MuLV in causing lymphoid transformation in vitro. A-MuLV-derived gag sequences could, however, functionally replace the 5' end of src and restore the transformation potential of a 5'-truncated src gene. To investigate this functional similarity, we replaced the gag sequence of an A-MuLV virus with the 5' end of src. This recombinant virus behaved like the A-MuLV virus from which it was derived: it transformed both fibroblasts and lymphoid cells in vitro. Taken together, these results suggest that lymphoid transformation in vitro is a specific property of abl and not of src or fps. Furthermore, it shows that a functional homology exists between the gag sequence of A-MuLV and the 5' end of src.
Mathey-Prevot
B
B
Baltimore
D
D
D000011:Abelson murine leukemia virus / Q000235:genetics; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002678:Chimera; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D051379:Mice; D008815:Mice, Inbred Strains; D009857:Oncogenes; D010957:Plasmids; D011494:Protein Kinases / Q000235:genetics*; D011505:Protein-Tyrosine Kinases; D012190:Retroviridae / Q000201:enzymology / Q000235:genetics*
D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D004262:DNA Restriction Enzymes