Purification and functional characterization of a cellular transcription factor that binds to an enhancer element within the adenovirus early EIIa promoter.
The adenovirus EIa-inducible early EIIa (EIIaE) promoter is comprised of several sequence elements essential for constitutive and induced expression. We report here the purification of the host-cell factor that interacts with the major upstream element of this promoter, extending between positions -90 and -70 with respect to the main EIIaE cap site and exhibiting enhancer properties. The purified factor, which corresponds to a 40- to 43-kDa polypeptide, specifically binds to its recognition site and stimulates EIIaE promoter activity when added to an in vitro transcription system, reconstituted from purified factors and RNA polymerase. The implication of this factor in the control of the other adenovirus early genes is discussed.
Jalinot
P
P
Centre National de la Recherche Scientifique, Unité 184 de Biologie Moléculaire et de Génie, Génétique, Faculté de Médecine, Strasbourg, France.
Wintzerith
M
M
Gaire
M
M
Hauss
C
C
Egly
J M
JM
Kédinger
C
C
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics; D002846:Chromatography, Affinity; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D004742:Enhancer Elements, Genetic; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000302:isolation & purification*; D014158:Transcription, Genetic
D017456:Adenovirus Early Proteins; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D014157:Transcription Factors