Enhancement of murine mixed lymphocyte response by 1,1-dimethylhydrazine: characterization and possible mechanism.
Treatment of mice with 1,1-dimethylhydrazine (UDMH) resulted in enhancement of the one-way mixed lymphocyte response (MLR); this effect was seen when both responder and stimulator mice were treated as well as when just the stimulator or just the responder mice were treated. Experiments in which splenocytes were exposed to UDMH in vitro indicated that exposure of the stimulator cells alone resulted in an enhanced MLR; exposure of the responder cells alone had no effect; and addition of UDMH to the assay (exposure of both populations) resulted in suppression of the response at higher concentrations. A possible mechanism for the enhancement of the MLR by UDMH was suggested by further experiments showing that UDMH inhibited prostaglandin E2 production by adherent splenocytes.
Tarr
M J
MJ
Department of Veterinary Pathobiology, Ohio State University, Columbus.
McKown
B J
BJ
Olsen
R G
RG
D000818:Animals; D004127:Dimethylhydrazines / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects*; D008763:Methylhydrazines / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D004127:Dimethylhydrazines; D008763:Methylhydrazines; C013958:dimazine; D015232:Dinoprostone