title: Cultured rat vascular smooth muscle cells: extracellular calcium and Na+-K+ regulation.
abstract: This study explores the relationship between extracellular calcium (Cao) and Na+-K+ regulation as it particularly pertains to the activity of the Na+ pump in cultured vascular smooth muscle cells (VSMCs) originating from Sprague-Dawley rats. As compared with cells incubated in media containing 0.5, 2.0, or 4.0 mM calcium, when the Na pump is active, VSMCs incubated in a Ca-deficient medium show a marked increase in intracellular sodium and no significant change in intracellular potassium. Associated with the rise in intracellular sodium there is an augmented activity of the Na pump. When the Na pump is inhibited, VSMCs incubated in either high-Ca medium (Cao = 4.0 mM) or Ca-deficient medium manifest a greater decline in intracellular potassium than cells incubated in media containing 0.5 or 2.0 mM calcium. Furthermore, when the Na pump is inhibited, VSMCs incubated in a Ca-deficient medium exhibit higher intracellular sodium levels in comparison with their counterparts incubated in media containing calcium. Flux experiments indicate that the aforementioned changes reflect increased membrane permeabilities to Na+ and K+. It is concluded that by regulating the permeability of the VSMC membrane, Cao plays an important role in the intracellular Na+-K+ homeostasis and that its effect on the Na pump is mediated via perturbations in the intracellular Na+ and K+ concentrations.
authors.forename: M
authors.initials: M
authors.lastname: Kino
authors.forename: A
authors.initials: A
authors.lastname: Tokushige
authors.forename: H
authors.initials: H
authors.lastname: Tamura
authors.forename: L
authors.initials: L
authors.lastname: Hopp
authors.forename: B M
authors.initials: BM
authors.lastname: Searle
authors.forename: F
authors.initials: F
authors.lastname: Khalil
authors.forename: A
authors.initials: A
authors.lastname: Aviv
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D005110:Extracellular Space / Q000378:metabolism; D008297:Male; D008433:Mathematics; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium; D002118:Calcium