Effect of changes in sodium balance on renin, angiotensinogen and atrial natriuretic factor messenger RNA levels in rats.
The expression of messenger (m)RNA for renin, angiotensinogen and atrial natriuretic factor (ANF) was investigated in rats on different sodium intakes. Messenger RNA was measured by a radiodensitometric hybridization assay. In the high-sodium state, renal renin mRNA decreased, but it increased in the low-sodium state. A further increase in renin mRNA was seen in the low-sodium state after captopril administration. Angiotensinogen mRNA levels in the liver, kidney and brain were altered by varying sodium intake. In the high-sodium state angiotensinogen mRNA decreased, but in the low-sodium state it increased. After treatment with captopril, angiotensinogen mRNA levels decreased in the liver and kidney. Angiotensinogen mRNA showed tissue specificity for expression, especially in the brain. Atrial ANF mRNA levels changed slightly with different levels of sodium intake.
Iwao
H
H
Department of Pharmacology, Kagawa Medical School, Japan.
Fukui
K
K
Kim
S
S
Nakayama
K
K
Ohkubo
H
H
Nakanishi
S
S
Abe
Y
Y
D000808:Angiotensinogen / Q000235:genetics*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D001921:Brain / Q000502:physiology*; D005786:Gene Expression Regulation; D007668:Kidney / Q000502:physiology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000097:blood / Q000235:genetics*; D012964:Sodium / Q000502:physiology*
D000808:Angiotensinogen; D009320:Atrial Natriuretic Factor; D012964:Sodium; D012083:Renin