Linear simian virus 40 DNA fragments exhibit a propensity for rolling-circle replication.
A linear simian virus 40 origin-containing DNA fragment replicated in monkey COS cells, generating tandemly repeated (head-to-tail) structures. Electron microscopy revealed circle-and-tail configurations characteristic of rolling-circle replication intermediates. Circularization of the same DNA before transfection led to a theta type of replication which generated supercoiled DNA molecules.
Deichaite
I
I
Laver-Rudich
Z
Z
Dorsett
D
D
Winocour
E
E
D000818:Animals; D002522:Chlorocebus aethiops; D004261:DNA Replication; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D008854:Microscopy, Electron; D013539:Simian virus 40 / Q000235:genetics*
D004270:DNA, Circular; D004279:DNA, Viral