title: Water deprivation upregulates angiotensin II receptors in rat anterior pituitary.
abstract: Angiotensin II (ANG II) receptors were quantitated in pituitary glands of individual male Long Evans rats by autoradiography after incubation of 8-microns thick pituitary sections with 125I-[Sar1]ANG II. Rat anterior pituitary had a single class of high-affinity saturable ANG II receptors with a Bmax of 1,360 +/- 109 fmol/mg protein and a Ka of 0.510 +/- 0.03 X 10(9) M-1. Five days of water deprivation produced a marked increase in the number of anterior pituitary ANG II receptors (Bmax: 2,428 +/- 233 fmol/mg protein, a 79% increase, P less than 0.001) and a decrease in affinity for the ligand (Ka: 0.337 +/- 0.01 10(9) M-1, a 34% decrease, P less than 0.05). Our results suggest a role for anterior pituitary ANG II receptors in the regulation of fluid and electrolyte metabolism in the rat.
authors.forename: A
authors.initials: A
authors.lastname: Israel
authors.forename: J M
authors.initials: JM
authors.lastname: Saavedra
authors.forename: L
authors.initials: L
authors.lastname: Plunkett
mesh_terms: D000804:Angiotensin II; D000818:Animals; D001345:Autoradiography; D008297:Male; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000032:analysis / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014868:Water Deprivation / Q000502:physiology*
chemical_list: D011945:Receptors, Angiotensin; D011956:Receptors, Cell Surface; D000804:Angiotensin II