Changes in intracellular redox and energy status during induced transplasma membrane electron transport in Cuscuta protoplasts.
Extracellular reduction of ferricyanide was exhibited by isolated Cuscuta protoplasts. A larger decrease in NADH than NADPH levels of the ferricyanide-treated protoplasts pointed to the major involvement of the former as an electron donor. Glutathione levels were also found to be lowered in similarly treated tissue. The time-dependent variation in intracellular ATP levels in presence of ferricyanide supported the concept of plasma membrane ATPase activation during transplasma membrane electron transport in eukaryotes.
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Department of Biochemistry, Lucknow University, India.
Misra
P C
PC
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004579:Electron Transport; D004734:Energy Metabolism; D005292:Ferricyanides / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010944:Plants / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism*
D005292:Ferricyanides; D009243:NAD; C007931:hexacyanoferrate III; D009249:NADP; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D005978:Glutathione