[Study of the mechanism of initiation of enzymatic NADPH-dependent lipid peroxidation in membranes of the endoplasmic reticulum].
The localization and mechanism of generation of active oxygen species in the enzymatic NADPH-dependent lipid peroxidation system in liver microsomes were studied. Using the spin-trapping method, the key role of active oxygen species in the initiation of NADPH-dependent enzymatic lipid peroxidation was confirmed. It was shown that active oxygen species are generated via consecutive one-electron reduction of the oxygen molecule by NADPH-cytochrome P-450 reductase.
Kagan
V E
VE
Serbinova
E A
EA
Minin
A A
AA
Savov
V M
VM
Novikov
K N
KN
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D003579:Cytochrome Reductases / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism*; D005609:Free Radicals; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000201:enzymology / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology
D005609:Free Radicals; D008054:Lipid Peroxides; D002374:Catalase; D013482:Superoxide Dismutase; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase
Izuchenie mekhanizma initsiirovaniia fermentativnogo NADPH-zavisimogo perekisnogo okisleniia lipidov v membranakh éndoplazmaticheskogo retikuluma.