title: Characterization of a hybridoma-derived T cell factor that promotes the production of antibodies bearing a dominant cross-reactive idiotype(s).
abstract: The participation of postulated subsets of T helper cells in antigen-specific antibody responses has generated both interest and controversy among immunologists. Specifically the import as well as the very existence of multiple populations of T helper cells has led to an intense search in recent years for cloned lines of such subsets that permit unambiguous classification and study. Furthermore, the means by which some of these T cells induce antibody responses may be via the elaboration of soluble factors mandating their characterization both biochemically and mechanistically. We have recently reported the existence of a T helper factor present in a 24-h Con A supernatant that specifically enhances an idiotype-bearing (Id+) response to trinitrophenol (TNP). The unique biochemical properties of this substance, namely, its capacity to bind both antigen and cross-reactive idiotype (CRI), has led to the generation of a cloned T cell hybridoma that constitutively "secretes" a factor which appears identical to the helper activity in Con A Sn. The cloned T cell hybridoma, herein designated LOP 1.4, elaborates a factor which selectively enhances the CRI+ anti-TNP antibody response in vitro. The specificity of the assay employed as well as its sensitivity for detecting significant enhancement of the percent CRI+ anti-TNP PFC response lent itself well as a useful vehicle for subsequent characterization of the factor. The LOP 1.4 factor, which can act at the later stages of the B cell response in a dose-dependent fashion, was characterized by affinity chromatography in order to probe the mechanism of its selective Id enhancement. The factor binds both the idiotype and the ligand for which one of the idiotype-bearing monoclonal antibodies is specific. That the factor binds idiotype and can be eluted selectively with ligand but not with noncross-reacting ligand suggests that the factor possesses separate but not independent binding sites, or alternatively, a single binding site that preferentially binds to a unique composite of antigen-idiotype. In addition, the factor bears I-J determinants, consistent with what we have previously detected on the surface of TH2-like cells. These results, collectively, suggest that the T cell hybridoma LOP 1.4 is a TH2-like cell (supporting the concept of multiple TH subsets) in light of its ability to enhance an idiotypic response to specific antigen through the production of a soluble factor that demonstrates affinity for both antigen and idiotype. In addition, like the I-J+ TH2 cell, the LOP 1.4 factor also bears I-J region determinants.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Microbiology, St. Louis University School of Medicine, MO 63104.
authors.forename: A M
authors.initials: AM
authors.lastname: Wardzala
authors.forename: M B
authors.initials: MB
authors.lastname: Bowen
authors.forename: G S
authors.initials: GS
authors.lastname: Jendrisak
authors.forename: C J
authors.initials: CJ
authors.lastname: Bellone
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D001665:Binding Sites; D003429:Cross Reactions; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis*; D015847:Interleukin-4; D007378:Interleukins / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation / Q000187:drug effects; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D000949:Histocompatibility Antigens Class II; C043911:I-J-antigen; D007130:Immunoglobulin Idiotypes; D007378:Interleukins; D015847:Interleukin-4