[The use of phage lambda promotors for expressing genes of secreted proteins in Bacillus subtilis cells].
At was shown with the help of promoterless alpha-amylase and staphylokinase genes that lambda PR and lambda PL promoters could be used in Bacillus subtilis. Promoters strength was compared to promoter of alpha-amylase gene, this enabled to order the promoters in a row: PAA greater than lambda PR greater than lambda PL. The lambda PR promoter region was controlled by temperature in E. coli cells only, but not in B. subtilis, therefore, the active lambda C1857 gene product was not produced in B. subtilis cells. The lambda PR promoter is used by B. subtilis at a later growth stage than PAA and the lambda PL promoter at a still later stage than lambda PR. The data enables lambda PR to be considered as quite useful for Bacilli.
Sorokin
A V
AV
Breitling
R
R
Behnke
D
D
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D010582:Bacteriophage lambda / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D008666:Metalloendopeptidases / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013696:Temperature; D014170:Transformation, Genetic; D000516:alpha-Amylases / Q000235:genetics
D001426:Bacterial Proteins; D000516:alpha-Amylases; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus
Ispol'zovanie promotorov faga lambda dlia ékspressii genov sekretiruemykh belkov v kletkakh Bacillus subtilis.