title: [Detection of anti-double stranded DNA antibodies of IgG, IgA and IgM classes by ELISA. Comparison with the Farr test and indirect immunofluorescence].
abstract: Anti-double stranded DNA antibodies were measured by an immunoglobulin class-specific immunoenzymatic assay (ELISA), in 450 sera from 265 patients as well as by indirect immunofluorescence using Crithidia luciliae as a substrate and, for 124 sera, by the Farr test. ELISA proved specific and reproducible and it yielded results that were well correlated with the Farr assay, with a slightly higher sensitivity of ELISA. Correlation with immunofluorescence was not as good because of the lower sensitivity of the latter method. ELISA enables the levels and isotypes of anti-DNA antibodies to be determined. Both appear to be critical parameters for a clinical interpretation of results, especially with respect to the diagnosis of systemic lupus erythematosus.
authors.affiliation: CNRS UA 1172, CHUR La Milétrie, Poitiers.
authors.forename: J L
authors.initials: JL
authors.lastname: Preud'homme
authors.forename: F
authors.initials: F
authors.lastname: Danon
authors.forename: E
authors.initials: E
authors.lastname: Rochard
authors.forename: D
authors.initials: D
authors.lastname: Gouet
authors.forename: G
authors.initials: G
authors.lastname: Touchard
authors.forename: P
authors.initials: P
authors.lastname: Aucouturier
authors.forename: M
authors.initials: M
authors.lastname: Alcalay
mesh_terms: D000974:Antibodies, Antinuclear / Q000032:analysis*; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D011863:Radioimmunoassay
chemical_list: D000974:Antibodies, Antinuclear; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004247:DNA
vernacular_title: Détection des anticorps anti-ADN natif de classes IgG, IgA et IgM par ELISA. Comparaison avec le test de Farr et l'immunofluorescence indirecte.