Lack of effect of insulin in hepatocytes isolated from streptozotocin-diabetic male rats.
Diabetes mellitus is known to affect drug and steroid metabolism in the rat liver. Recently we have demonstrated that in-vitro insulin addition to hepatocytes obtained from normal male rats showed a significant dose-related increase in androstenedione metabolism. We have extended our study this time by using 3- and 21-days streptozotocin (STZ) diabetic and insulin-treated STZ-diabetic male rats. Hepatocytes from 3- and 21-days STZ-diabetic rats were resistant to the effect of insulin while insulin-treated diabetic rats indicated partial restoration of insulin effect. Since insulin resistance is a characteristic feature of type II diabetes mellitus, we would like to suggest that STZ-diabetic rats may be a model for type II diabetes mellitus.
Hussin
A H
AH
Department of Pharmacology, The University, Glasgow, Scotland, U.K.
Skett
P
P
D000735:Androstenedione / Q000378:metabolism*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000503:physiopathology*; D013250:Steroid Hydroxylases / Q000378:metabolism; D013311:Streptozocin
D007328:Insulin; D000735:Androstenedione; D013311:Streptozocin; D013250:Steroid Hydroxylases