title: Effects of tunicamycin on NGF binding and neurite outgrowth in PC12 cells.
abstract: The effects of inhibition of glycosylation on nerve growth factor (NGF) binding and neurite outgrowth response of PC12 cells have been examined. Exposure to tunicamycin (1-10 micrograms/ml) for 24-36 hr eliminates the rapidly dissociating component of NGF binding and decreases the proportion of PC12 cells capable of elaborating neurites in a dose-dependent manner. These decreased cellular responses are probably due to an underglycosylation of the NGF receptor, since the effects of tunicamycin are correlated with a decrease in 3H-fucose incorporation rather than a general decline in cellular metabolism as measured by viability and protein synthesis. These results suggest that carbohydrate side chains are important for the function and/or orientation of the NGF receptor in PC12 cells and that the rapidly dissociating component of NGF binding may be associated with a minimum concentration of functional receptors per cell required for the full biologic response.
authors.forename: T J
authors.initials: TJ
authors.lastname: Baribault
authors.forename: K E
authors.initials: KE
authors.lastname: Neet
mesh_terms: D000310:Adrenal Gland Neoplasms; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004347:Drug Interactions; D005944:Glucosamine / Q000031:analogs & derivatives*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D010673:Pheochromocytoma; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects*; D017475:Receptors, Nerve Growth Factor; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D014415:Tunicamycin; D005944:Glucosamine