title: Endothelial cell adhesion to vascular prosthetic surfaces.
abstract: Improving the long term patency of small diameter prosthetic grafts remains an important but elusive objective in vascular surgery. In pursuit of a non-thrombogenic surface, we have cultured human-adult endothelial cells, examined their adhesive properties and their ability to colonize the inner surface of a Dacron graft. To examine cell adhesion, endothelial cells were labelled with 111In-oxine and inoculated onto prosthetic wells previously prepared with either cold insoluble globulin (CIG), 1% gelatin, alginate or left untreated as a control. At 100 min, mean percentage adhesion to CIG and gelatin precoated wells was 86.0 +/- 9.9% (+/- SD) and 81.6 +/- 2.9% respectively, whereas alginate at 57.5 +/- 7.5% and the control well at 48.3 +/- 9.9% showed significantly less adhesion. Further experiments examined the adhesion of Indiumoxine labelled endothelial cells to Dacron graft (2 cm X 6 mm ID) placed in an in vitro arterial circuit. An immediate loss of approximately 20% of the cells occurred within the first 30 s, whereafter, a stable population were adherent to the graft material. By 102 min, 73.4 +/- 1.8% of cells remained attached when exposed to tissue culture medium, but only 64.1 +/- 6.9% after exposure to blood. Cultured human adult endothelial cells adhere most effectively to prosthetic surfaces precoated with CIG or gelatin, and remain attached following exposure to shear forces.
authors.affiliation: Department of Surgery and Pathology, East Birmingham Hospital, UK.
authors.forename: D
authors.initials: D
authors.lastname: Gourevitch
authors.forename: C E
authors.initials: CE
authors.lastname: Jones
authors.forename: J
authors.initials: J
authors.lastname: Crocker
authors.forename: M
authors.initials: M
authors.lastname: Goldman
mesh_terms: D000464:Alginates; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D005353:Fibronectins; D005780:Gelatin; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D007205:Indium Radioisotopes; D015125:Oxyquinoline; D011093:Polyethylene Terephthalates; D014654:Vascular Patency
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D005353:Fibronectins; D006603:Hexuronic Acids; D007205:Indium Radioisotopes; D011093:Polyethylene Terephthalates; D015125:Oxyquinoline; D020723:Glucuronic Acid; D005780:Gelatin