Fe3+-hydroxamate as immobilized metal affinity-adsorbent for protein chromatography.
The adsorbent glycinehydroxamate-Sepharose 6B, charged with Fe3+ under specified conditions, is reported. It was used at various pH values for chromatography of the following proteins: lysozyme, cytochrome c, avidin, bovine pancreatic RNase, myoglobin, ovalbumin and human serum albumin. The common naturally occurring amino acids were tested for their interactions with the new sorbent under neutral conditions.
Ramadan
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Porath
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D000327:Adsorption; D000596:Amino Acids / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D003574:Cytochrome c Group / Q000302:isolation & purification; D005291:Ferrichrome; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids; D007501:Iron; D008670:Metals; D009113:Muramidase / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*
D000596:Amino Acids; D003574:Cytochrome c Group; D006877:Hydroxamic Acids; D008670:Metals; D011506:Proteins; D005291:Ferrichrome; D007501:Iron; D009113:Muramidase