Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor.
A mouse monoclonal IgG2a antibody, designated MRC OX-26, is shown to be specific for the rat transferrin receptor, but does not block transferrin binding. The antibody labelled a myeloma, three leukaemia cell lines and normal dividing cells of various types, but also bound to a number of nondividing normal tissues. No labelling of lymphopoietic stem cells could be detected, even though approximately 25% of bone marrow and over 95% of fetal liver cells were clearly labelled.
Jefferies
W A
WA
Brandon
M R
MR
Williams
A F
AF
Hunt
S V
SV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis; D001666:Binding Sites, Antibody; D001854:Bone Marrow Cells; D002469:Cell Separation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D008099:Liver / Q000166:cytology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000276:immunology*; D011990:Receptors, Transferrin; D014168:Transferrin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D014168:Transferrin