Atrial natriuretic factor inhibits norepinephrine release in an adrenergic clonal cell line (PC12).
Evidence for a presynaptic neuromodulatory effect of atrial natriuretic factor (ANF) has been obtained in rat adrenal pheochromocytoma cells (PC12), a clonal line that differentiates into adrenergic neuron-like cells when treated with nerve growth factor. ANF had no effect on basal norepinephrine (NE) release. In contrast, ANF markedly and significantly inhibited carbachol-induced NE release in a concentration-dependent manner. This result is consistent with the hypothesis that ANF may be an inhibitory neuromodulator.
Drewett
J
J
Department of Pharmacology, School of Medicine, University of Minnesota, Duluth 55812.
Marchand
G
G
Ziegler
R
R
Trachte
G
G
D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism*; D010673:Pheochromocytoma / Q000378:metabolism; D051381:Rats; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D009320:Atrial Natriuretic Factor; D002217:Carbachol; D009638:Norepinephrine