Immortalization of human lymphocytes by co-cultivation with lethally irradiated autologous T-cell lines harbouring human T-cell leukaemia virus-I.
Human adult peripheral blood lymphocytes were successfully immortalized by co-cultivation with irradiated autologous and homologous T-cell lines harbouring human T-cell leukaemia virus-I (HTLV-I). The efficiency of transformation was the same in both cases. The participation of alloantigen stimulation in co-cultivation procedures is discussed, and it is stressed that factors other than alloantigen stimulation might be required for the efficient immortalization of human T-lymphocytes in vitro by HTLV.
Akagi
T
T
Ohtsuki
Y
Y
Takahashi
K
K
Takeda
I
I
Oka
T
T
Miyoshi
I
I
D000328:Adult; D002460:Cell Line; D002472:Cell Transformation, Viral; D017977:Deltaretrovirus / Q000254:growth & development*; D006801:Humans; D007519:Isoantigens; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D013601:T-Lymphocytes / Q000382:microbiology* / Q000528:radiation effects
D007519:Isoantigens