title: A drug-resistant mutation in the ribosomal DNA of Tetrahymena.
abstract: A mutation that confers resistance to the drug paromomycin is shown to be in the structural gene that codes for the ribosomal RNA in Tetrahymena. This observation was made by exploiting a variant of the ribosomal DNA that distorts amplification of this locus when a new somatic nucleus develops during conjugation. Because the allelic forms of this locus have a restriction endonuclease site polymorphism, it was possible to correlate drug resistance with presence of a specific allele. The genetic results have been confirmed by sequence analysis (presented elsewhere). Thus, the crosses presented here provide a unique opportunity to identify mutations in the ribosomal DNA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Bruns
authors.forename: A L
authors.initials: AL
authors.lastname: Katzen
authors.forename: L
authors.initials: L
authors.lastname: Martin
authors.forename: E H
authors.initials: EH
authors.lastname: Blackburn
mesh_terms: D000483:Alleles; D000818:Animals; D004262:DNA Restriction Enzymes; D004275:DNA, Ribosomal / Q000235:genetics*; D004352:Drug Resistance, Microbial; D005784:Gene Amplification; D005796:Genes; D009154:Mutation; D010303:Paromomycin / Q000494:pharmacology; D013768:Tetrahymena / Q000187:drug effects / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D010303:Paromomycin; D004262:DNA Restriction Enzymes