title: Crystallization and preliminary X-ray studies of T4 phage beta-glucosyltransferase.
abstract: Crystals of the DNA glucosylating enzyme beta-glucosyltransferase from phage T4 have been grown in the presence of uridine diphosphate glucose. The crystals are orthorhombic, space group P2(1)2(1)2 with a = 148.3 A, b = 52.6 A, c = 52.6 A. The assumption of one monomer of Mr 40,000 per asymmetric unit gives rise to a Vm of 2.56 A 3/dalton. The crystals diffract to beyond 2.7 A and are suitable for X-ray structure analysis.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
authors.forename: P S
authors.initials: PS
authors.lastname: Freemont
authors.forename: W
authors.initials: W
authors.lastname: Rüger
mesh_terms: D003460:Crystallization; D005964:Glucosyltransferases; D013604:T-Phages / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D005964:Glucosyltransferases; C026585:DNA beta-glucosyltransferase