Molecular cloning of a full-length cDNA encoding the hemagglutinin-neuraminidase glycoprotein of Sendai virus.
We cloned a full-length complementary DNA for the hemagglutinin-neuraminidase (HN) mRNA of Sendai virus (HVJ) using a synthetic 27-mer as a probe. Nucleotide sequence analysis showed that there is a long open reading frame on the mRNA that encodes a protein of 575 amino acids. The deduced amino acid sequence indicated that only one hydrophobic region sufficiently long to anchor the protein in the membrane and located near the N-terminus (amino acids 35-60). It is suggested that HN protein is oriented with its N-terminus inside the membrane.
Miura
N
N
Nakatani
Y
Y
Ishiura
M
M
Uchida
T
T
Okada
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D015707:HN Protein; D010222:Parainfluenza Virus 1, Human / Q000201:enzymology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D004274:DNA, Recombinant; D015707:HN Protein; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D004247:DNA