title: Analysis of HSV isolated from patients with unilateral and bilateral herpetic keratitis.
abstract: Restriction enzyme analysis was utilized to investigate 11 HSV isolates from patients with herpetic keratitis. The electrophoretic profiles of the DNAs indicate that the isolates belong to HSV-1 and are epidemiologically unrelated. Two of the isolates, derived from a patient with simultaneous bilateral herpetic keratitis were further characterized by genetic and phenotypic analyses. These studies showed the following: (i) the two recrudescent lesions were caused by the same HSV-1 isolate; (ii) the isolate yielded two different plaque phenotypes; (iii) these plaques showed different sensitivity to I.D.U.
authors.forename: M
authors.initials: M
authors.lastname: Tognon
authors.forename: R
authors.initials: R
authors.lastname: Manservigi
authors.forename: A
authors.initials: A
authors.lastname: Sebastiani
authors.forename: G
authors.initials: G
authors.lastname: Bragliani
authors.forename: M
authors.initials: M
authors.lastname: Busin
authors.forename: E
authors.initials: E
authors.lastname: Cassai
mesh_terms: D000212:Acyclovir / Q000627:therapeutic use; D000328:Adult; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D004352:Drug Resistance, Microbial; D004586:Electrophoresis; D006561:Herpes Simplex; D006801:Humans; D007065:Idoxuridine / Q000627:therapeutic use; D007634:Keratitis / Q000188:drug therapy / Q000209:etiology* / Q000382:microbiology; D008297:Male; D018139:Simplexvirus / Q000032:analysis / Q000145:classification / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D007065:Idoxuridine; D000212:Acyclovir