Alkaline deoxyribonuclease induced with diterpene ester TPA and n-butyrate in Epstein-Barr virus genome-carrying Raji cells.
The combination of 12-O-tetradecanoylphorbol-13-acetate and n-butyrate induced Epstein-Barr virus (EBV)-associated alkaline DNase in Raji cells. The anti-early-antigen-positive sera neutralized EBV-associated DNase activity. The enzyme activity was eluted at about 0.20 and 0.24 M KCl from DEAE-cellulose and phosphocellulose columns, respectively. The partially purified enzyme was sensitive to (NH4)2SO4 and spermine. Immunological and biochemical properties of EBV-associated DNase induced in Raji cells were similar to those of the enzyme induced in P3HR-1 cells.
Kawanishi
M
M
Ito
Y
Y
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D002848:Chromatography, DEAE-Cellulose; D003851:Deoxyribonucleases / Q000032:analysis / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D004854:Herpesvirus 4, Human; D006801:Humans; D010704:Phorbols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002087:Butyrates; D010704:Phorbols; D003851:Deoxyribonucleases; D013755:Tetradecanoylphorbol Acetate