Two related but differentially expressed potential membrane proteins encoded by the EcoRI Dhet region of Epstein-Barr virus B95-8.
Three mRNAs in the EcoRI Dhet region of Epstein-Barr virus B95-8 were mapped. Their 3' ends are coterminal. A latent gene containing three exons is transcribed from the ED-L1 promoter and was predicted to lead to expression of a 42-kilodalton protein. An unspliced late mRNA is produced by transcription from the ED-L1A promoter within the first intron of the above gene and was predicted to lead to expression of a 28-kilodalton protein corresponding to the C-terminal two-thirds of the 42-kilodalton protein. Both proteins would have hydrophobic N-terminal domains and highly acidic C-terminal domains and were predicted to span the cell membrane. An early promoter (ED-L2) is located in the 3' untranslated region of the above genes and was predicted to lead to expression of a 6.5-kilodalton polypeptide containing a C-terminal hydrophobic region.
Hudson
G S
GS
Farrell
P J
PJ
Barrell
B G
BG
D001483:Base Sequence; D002462:Cell Membrane / Q000032:analysis; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*
D004279:DNA, Viral; D008565:Membrane Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI