[Effects of synthetic bioactive lipids on the activity of D-beta-hydroxybutyrate dehydrogenase, a membrane enzyme].
The structural requirements of lecithins analogs for purified D-beta-hydroxybutyrate dehydrogenase activation have been studied with chemically defined phospholipids. It appears that the trimethylamine group of choline can be changed by a pyridinium group. On the other hand, the decrease of density of the positive charges on the liposomes surface obtained by dilution of such bearing molecules with negative or non charged phospholipids increases the enzyme reactivation. Finally, the PAF acether, a lipid mediator, is able to reactivate the enzyme in similar conditions as these obtained with mitochondrial phosphatidylcholines.
Nasser
B
B
Université de Franche-Comté, Laboratoire de Biochimie et Biologie Moléculaire (UA CNRS 531), Besançon, France.
Morpain
C
C
Laude
B
B
Latruffe
N
N
D055598:Chemical Phenomena; D002621:Chemistry; D004789:Enzyme Activation; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology; D008081:Liposomes / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000494:pharmacology
D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D010972:Platelet Activating Factor; D006884:Hydroxybutyrate Dehydrogenase
Effets de lipides bioactifs synthétiques sur l'activité de la D-beta-hydroxybutyrate déshydrogénase, une enzyme membranaire.