The metabolism of leukotrienes in blood plasma studied by high-performance liquid chromatography.
The metabolism of leukotrienes (B4, C4, D4, and E4) within human plasma was studied and a simple sample preparation is presented. It was demonstrated that leukotriene E4 and leukotriene B4 were stable during incubation at 37 degrees C using the in vitro system. In contrast, leukotriene C4 was metabolized by gamma-glutamyl transpeptidase activities into leukotriene D4 which was further metabolized by dipeptidase activities of plasma into leukotriene E4. The transition state inhibitor of gamma-glutamyl transpeptidase L-serine-borate decreased the metabolism of leukotriene C4 in plasma. Dilution of plasma demonstrated that the dipeptidase was more active compared to the gamma-glutamyl transpeptidase. The metabolizing activities of plasma were functionally characterized by fractionating the plasma proteins.
Köller
M
M
Schönfeld
W
W
Knöller
J
J
Bremm
K D
KD
König
W
W
Spur
B
B
Crea
A
A
Peters
W
W
D001798:Blood Proteins / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006801:Humans; D007201:Indicator Dilution Techniques; D007975:Leukotriene B4 / Q000097:blood*; D017999:Leukotriene E4; D011489:Protein Denaturation; D013189:SRS-A / Q000031:analogs & derivatives* / Q000097:blood*
D001798:Blood Proteins; D013189:SRS-A; D007975:Leukotriene B4; D017999:Leukotriene E4