Increased methotrexate-induced chromosome breakage in patients with free trisomy 21 and their parents.
Increased susceptibility of chromosomes from peripheral blood lymphocytes to the antimetabolite methotrexate (2 X 10(-6) M) has been found in patients with free trisomy 21 and their parents (N = 14). The level of induced chromatid and chromosome breaks is lowest in normal controls intermediate in patients' mothers and fathers, and highest in trisomy 21 patients. The findings are viewed as a special type of cytogenetic polymorphism or as a defective chromosomal infrastructure, also in the parents of trisomic children.
Pelz
L
L
Abteilung für Neonatologie und Klinische Genetik, Universitäts-Kinderklinik, Wilhelm-Pieck Universität, Rostock, German Democratic Republic.
Götz
J
J
Krüger
G
G
Witt
G
G
D000328:Adult; D002478:Cells, Cultured; D002675:Child, Preschool; D002873:Chromosome Fragility; D002891:Chromosomes, Human, Pair 21; D004314:Down Syndrome / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D010290:Parents; D011110:Polymorphism, Genetic
D008727:Methotrexate