Chronic inhalation of short asbestos fibers.
An animal inhalation study was initiated to study the chronic biological effects of inhalation of short chrysotile asbestos fibers. Rats and monkeys were exposed for 18 months, 7 hr/day, 5 days/week to a specially prepared, chrysotile asbestos aerosol. Based upon daily chamber measurements, the mean concentration of fibers in the chamber air was 1.0 mg/m3. By phase contrast microscopy, the number of fibers greater than 5 micron in length was determined to be 0.79 fiber per cubic centimeter. Rats were autopsied for pathological and histochemical examination at 1, 3, 6, 12, 18, and 24 months after initiating exposures. No significant differences in the histochemical data were seen between the exposed and control groups. Gross and histopathologic examination of exposed and control groups of rats indicated no compound-related lesions, including fibrosis. Open lung biopsies were performed on the chrysotile-exposed and the control monkeys 28 months after initiating exposures. Histopathologic evaluation of the lung biopsy tissue showed the presence of asbestos bodies adjacent to the terminal bronchioles of the asbestos-exposed monkeys. There was no observed fibrosis in pulmonary tissue. All monkeys are being maintained for an indefinite period and observed for signs of latent pulmonary disease.
Platek
S F
SF
Groth
D H
DH
Ulrich
C E
CE
Stettler
L E
LE
Finnell
M S
MS
Stoll
M
M
D000336:Aerosols; D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D017632:Asbestos, Serpentine; D001835:Body Weight / Q000187:drug effects; D004391:Dust; D008168:Lung / Q000648:ultrastructure; D008171:Lung Diseases / Q000209:etiology / Q000473:pathology; D008252:Macaca fascicularis; D008297:Male; D008855:Microscopy, Electron, Scanning; D009929:Organ Size / Q000187:drug effects; D010316:Particle Size; D051381:Rats; D011919:Rats, Inbred Strains; D012825:Silicon / Q000097:blood / Q000378:metabolism; D013045:Species Specificity; D013997:Time Factors
D000336:Aerosols; D017632:Asbestos, Serpentine; D004391:Dust; D001194:Asbestos; D012825:Silicon