title: Experimental myocardial ischemia. Differential injury of mitochondrial subpopulations.
abstract: Distinct populations of subsarcolemmal (SS) and inter-fibrillar (IF) rat cardiac mitochondria were studied following 15 and 30 minutes of warm and cold global ischemia. The respiratory control index, state 3, state 4, adenosine diphosphate-oxygen ratio, and specific enzyme activities of these mitochondrial populations were examined. The subsarcolemmar and IF mitochondria were both severely uncoupled and inhibited by warm ischemia. However, IF mitochondria had a higher RCI at each ischemic interval. In cold ischemia, IF mitochondria were not injured compared with control specimens. Subsarcolemmar mitochondria showed a trend towards a lower RCI that was statistically significant at 30 minutes with succinate as a substrate. These data implicate a differential injury of ischemia on the compartmentalized bioenergy metabolism of the myocardial cell.
authors.forename: E S
authors.initials: ES
authors.lastname: Weinstein
authors.forename: D W
authors.initials: DW
authors.lastname: Benson
authors.forename: D J
authors.initials: DJ
authors.lastname: Ratcliffe
authors.forename: J
authors.initials: J
authors.lastname: Maksem
authors.forename: D E
authors.initials: DE
authors.lastname: Fry
mesh_terms: D000818:Animals; D003080:Cold Temperature; D003327:Coronary Disease / Q000378:metabolism* / Q000473:pathology; D003402:Creatine Kinase / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D006358:Hot Temperature; D066298:In Vitro Techniques; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007521:Isocitrate Dehydrogenase; D003576:Electron Transport Complex IV; D003402:Creatine Kinase