Production of and response to interleukin-2 by cultured T cells: effects of lithium chloride and other putative modulators.
Immunomodulating substances may be useful as host-modifying adjuvants in patients receiving immunosuppressive therapies or as probes for studying the immune system. Detection of interleukin-2 (IL-2) is possible via a microassay, enabling investigation of the effects of immunomodulating agents on T cell production of and response to IL-2 in vitro. Four such agents were examined: lithium chloride, Bestatin, Azimexon, and muramyl dipeptide (MDP). All four agents increased the response of cultured T cells to IL-2 over control populations supplied with IL-2 alone. Lithium chloride, Bestatin, and MDP enhanced the production of IL-2 in standard mixed lymphocyte cultures over control cultures receiving media alone. These results suggest that several immunomodulators may produce their effects by the modulation of IL-2 or other lymphokines. Additionally, microassay systems may prove useful as probes to elucidate the function of putative immunoregulatory substances.
Kishter
S
S
Hoffman
F A
FA
Pizzo
P A
PA
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001388:Aziridines / Q000494:pharmacology; D002460:Cell Line; D002712:Chlorides / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
D000276:Adjuvants, Immunologic; D001388:Aziridines; D002712:Chlorides; D007376:Interleukin-2; D010835:Phytohemagglutinins; C016866:azimexon; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D008094:Lithium; D018021:Lithium Chloride; D007930:Leucine; C012211:ubenimex