Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase.
The yeast GDH1 gene encodes NADP-dependent glutamate dehydrogenase. This gene was isolated by complementation of an Escherichia coli glutamate auxotroph. NADP-dependent glutamate dehydrogenase was overproduced 6-10-fold in Saccharomyces cerevisiae bearing GDH1 on a multicopy plasmid. The nucleotide sequence of the 1362-base pair coding region and 5' and 3' flanking sequences were determined. Transcription start sites were located by S1 nuclease mapping. Regulation of GDH1 was not maintained when the gene was present on a multicopy plasmid. Protein secondary structure predictions identified a region with potential to form the dinucleotide-binding domain. The amino acid sequences of the yeast and Neurospora crassa enzymes are 63% conserved. Unlike the N. crassa gene, yeast GDH1 has no introns.
Moye
W S
WS
Amuro
N
N
Rao
J K
JK
Zalkin
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D004720:Endonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D018086:Glutamate Dehydrogenase (NADP+) / Q000235:genetics*; D009249:NADP / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D009249:NADP; D004247:DNA; D005969:Glutamate Dehydrogenase; C000609733:GDH1 protein, S cerevisiae; D018086:Glutamate Dehydrogenase (NADP+); D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015719:Single-Strand Specific DNA and RNA Endonucleases