IL-1 secretion and membrane IL-1 expression by neonatal spleen cells during soluble antigen presentation.
Antigen presentation and IL-1 production by neonatal spleen cells were studied in a murine model. The T-helper-cell line (D10-G4.1) (D10), which is specific for soluble antigen presented on syngeneic antigen-presenting cells and dependent on IL-1 for its proliferation, was used as an indicator cell for the ability of syngeneic neonatal or adult spleen cells to present antigen and produce IL-1. The antigen-presenting capacity of neonatal spleen cells is low as attested by D10 proliferation. During antigen presentation there is an augmentation of IL-1 production by the antigen-presenting spleen cell population. However, neonatal spleen cells do not respond to the same levels as do adult spleen cells. These reduced levels of secreted IL-1 cannot be attributed to a low potential for producing IL-1 as attested by the high levels of IL-1 made by these cells after induction by a crude IL-1 inducer factor (IL-1-IF) and by the stimulus of the IL-1-IF produced by D10 cells during antigen presentation by paraformaldehyde-fixed adult cells. The spontaneous expression of membrane IL-1 by neonatal cells is low. Membrane IL-1 levels on neonatal cells can be brought to adult levels by induction with IL-1-IF. Neonatal spleen cells have an impaired capacity to process and/or present soluble antigen. This impairment leads to a decreased stimulus of the T helper cell to produce inducer factors and thus a reduced level of IL-1 production by the neonatal cells during antigen presentation.
Levin
D
D
Department of Immunology, Technion Faculty of Medicine, Haifa, Israel.
Gershon
H
H
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001685:Biological Factors / Q000096:biosynthesis / Q000502:physiology; D002460:Cell Line; D007375:Interleukin-1 / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology
D001685:Biological Factors; D007375:Interleukin-1; D008565:Membrane Proteins