Inhibitors of ADP-ribosyl transferase enhance the transformation of NIH3T3 cells following transfection with SV40 DNA.
The influence of inhibitors of the enzyme ADP-Ribosyl Transferase (ADPRT) upon the morphological transformation of NIH3T3 cells by calcium phosphate-SV40 DNA co-precipitates was examined. Marked enhancement in the frequency of foci formation was noted when 3-methoxybenzamide was added to cells either during or after exposure to SV40 DNA, but there was no effect when cells were only pretreated with the inhibitor. The greatest enhancement was observed when cells were exposed to inhibitor both during and after transfection with DNA. The involvement of ADPRT activity in the events during transfection of DNA into cells is not understood. These results, however, highlight the importance of the key regulatory molecule poly (ADP-ribose) in the events which occur within the nucleus and which lead to transformation with SV40 DNA.
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D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002472:Cell Transformation, Viral; D004279:DNA, Viral; D004305:Dose-Response Relationship, Drug; D051379:Mice; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D011065:Poly(ADP-ribose) Polymerases; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
D001549:Benzamides; D004279:DNA, Viral; D011065:Poly(ADP-ribose) Polymerases; D009713:Nucleotidyltransferases; C025159:3-methoxybenzamide