title: Structure of the penicillin acylase gene from Escherichia coli: a periplasmic enzyme that undergoes multiple proteolytic processing.
abstract: Penicillin acylase is processed from a 90-kD precursor through the cleavage of a leader peptide and two further endopeptidase cleavages to yield an enzyme that contains a 22-kD (or 23-kD) and a 65-kD subunit. The endopeptidase cleavages require an intact carboxy terminus. This type of processing appears to be unique for a prokaryotic enzyme, having its most closely related analog in the synthesis and processing of preproinsulin and other eukaryotic hormones.
authors.forename: W
authors.initials: W
authors.lastname: Bruns
authors.forename: J
authors.initials: J
authors.lastname: Hoppe
authors.forename: H
authors.initials: H
authors.lastname: Tsai
authors.forename: H J
authors.initials: HJ
authors.lastname: Brüning
authors.forename: F
authors.initials: F
authors.lastname: Maywald
authors.forename: J
authors.initials: J
authors.lastname: Collins
authors.forename: H
authors.initials: H
authors.lastname: Mayer
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D010399:Penicillin Amidase / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational
chemical_list: D004251:DNA Transposable Elements; D000581:Amidohydrolases; D010399:Penicillin Amidase