Liquid chromatographic analysis of doxazosin in human serum with manual and robotic sample preparation.
A specific method for the determination of the antihypertensive drug doxazosin in human serum is described. The method utilizes the related drug prazosin as an internal standard and is based on a simple extraction scheme followed by analysis by reversed-phase ion suppression high-performance liquid chromatography (HPLC) on an alumina-based column with fluorescence detection. The method is completely automated with a flexible robotic system for the analysis of drugs in biological fluids. The robotic automation of the method allows a 20% increase in the sample throughput and the savings of about 7 man-hours a day. Both the manual and robotic procedures yield precise quantitative results over the therapeutically relevant concentration range of 0.5 to 20 ng/mL of serum.
Fouda
H G
HG
Central Research, Pfizer Inc., Groton, Connecticut 06340.
Twomey
T M
TM
Schneider
R P
RP
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D017292:Doxazosin; D006801:Humans; D011224:Prazosin / Q000031:analogs & derivatives* / Q000097:blood; D012371:Robotics; D013050:Spectrometry, Fluorescence
D017292:Doxazosin; D011224:Prazosin