[Experimental conditions of a technic of in vitro transcription using placental nuclei].
To examine the expression of human placental lactogen, we investigated transcription in isolated placental nuclei. Using SH-agarose affinity chromatography, we have isolated Hg-RNA synthetized in vitro. These RNA are translated in a wheat germ cell-free system. hPL synthetized in vitro, accounting for 20% of total protein. This system should prove useful for the study of gene expression of hPL.
Hess
K
K
Laboratoire de Biochimie Médicale I, Faculté de Médecine, Vandaeuvre-les-Nancy.
el Ouardirhi
H
H
Belleville
F
F
Paysant
P
P
Nabet
P
P
D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008722:Methods; D010928:Placental Lactogen / Q000235:genetics*; D011247:Pregnancy; D014176:Protein Biosynthesis; D015138:RNA, Nuclear / Q000235:genetics*; D014158:Transcription, Genetic
D015138:RNA, Nuclear; D010928:Placental Lactogen
Etude des conditions expérimentales d'une technique de transcription in vitro utilisant des noyaux de placenta.