Liposomes in chemo- and immunotherapy of cancer.
In this paper, we report on the in vivo behavior of liposomes as a function of their size and composition. It is emphasized that by varying these parameters we can influence not only the rate of blood elimination but also the intrahepatic destination of the liposomes. Thus, we show that small liposomes with diameters well below 100 nm can reach and be internalized by the parenchymal cells of the liver, i.e. the hepatocytes. The rate and the extent at which this occurs depends on the liposomal composition. With respect to the application of liposomes as a drug carrier system in anticancer therapy, we put emphasis on the liver macrophage, i.e. the Kupffer cell, as a target cell. Large liposomes with diameters well over 100 nm exclusively are taken up by these cells as far as hepatic uptake is concerned. By encapsulation within liposomes, a drug may be delivered specifically to these macrophages; this will prevent its rapid excretion from the body and/or undesired accumulation in other cell types. Two examples of the way in which this condition may be exploited are presented. First, we demonstrate the formation of intracellular depots in the macrophages of the cytostatic drug 5-fluorodeoxyuridine (FUdR), thus preventing the rapid metabolism of the drug by the hepatocytes and allowing its sustained release from the macrophages and subsequent uptake by adjacent metastatic tumor cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Scherphof
G L
GL
Laboratory of Physiological Chemistry, University of Groningen, The Netherlands.
Daemen
T
T
Spanjer
H H
HH
Roerdink
F H
FH
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000493:pharmacokinetics / Q000627:therapeutic use*; D000230:Adenocarcinoma / Q000188:drug therapy* / Q000556:secondary; D000818:Animals; D005467:Floxuridine / Q000493:pharmacokinetics / Q000627:therapeutic use*; D008081:Liposomes / Q000008:administration & dosage* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000556:secondary; D008262:Macrophage Activation / Q000187:drug effects*; D051381:Rats; D013936:Thymidine
D008081:Liposomes; D005467:Floxuridine; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D013936:Thymidine