title: Suicide vector for transposon mutagenesis in Pseudomonas solanacearum.
abstract: A suicide vector was constructed by cloning the transfer genes of the wide-host-range (IncW group) plasmid R388 into the BamHI site of pBR325. This plasmid can deliver Tn5 into Pseudomonas solanacearum at frequencies ranging from 10(-6) to 10(-9) per recipient.
authors.forename: V M
authors.initials: VM
authors.lastname: Morales
authors.forename: L
authors.initials: L
authors.lastname: Sequeira
mesh_terms: D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D005822:Genetic Vectors; D009154:Mutation; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes