title: DNA binding properties of murine p53.
abstract: We analysed the in vitro binding of p53 from normal (3T3) and from chemically transformed (Meth A) Balb/c mouse cells to double-stranded (ds-) DNA and to single-stranded (ss-) DNA by DNA-cellulose chromatography. We confirm previous findings that p53 in cellular extracts exhibits ds-DNA-binding activity (Lane and Gannon, 1983). In addition, we demonstrate that such p53 also binds to ss-DNA. Analyses with immunopurified p53 protein provide evidence that this DNA-binding activity is intrinsic to p53. DNA binding of p53 could not be inhibited by a monoclonal antibody specific for the C-terminal region. An N-terminal deletion mutant of p53 (Rovinski et al., 1987) exhibited similar DNA-binding properties as wild-type p53, indicating that the N-terminus also is dispensable for DNA binding. We further show a close correlation between the DNA-binding activity of p53 from 3T3 cells and its association with nuclear substructures.
authors.affiliation: Department of Biochemistry, University of Ulm, Federal Republic of Germany.
authors.forename: K
authors.initials: K
authors.lastname: Steinmeyer
authors.forename: W
authors.initials: W
authors.lastname: Deppert
mesh_terms: D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D015513:Oncogene Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D004268:DNA-Binding Proteins; D015513:Oncogene Proteins; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53