Phorbol ester stimulation of fibronectin-mediated cell adhesion.
Stimulation of Chinese hamster ovary cells with 12-O-tetradecanoyl 13-acetate increases the rate of adhesion to fibronectin-coated substratum. The EC50 of the phorbol ester that initiates the change in kinetics of adhesion is approximately 8 nM and is specific to those phorbol esters which activate protein kinase C. When compared to control cells, cells stimulated with active phorbol esters require a significantly lower amount of fibronectin to support their adhesion, and exhibit 50% adhesion inhibition by a log-fold higher concentration of PB1, a monoclonal antibody which specifically blocks fibronectin-mediated adhesion. These results indicate that stimulation of cells with phorbol esters results in a modification of the fibronectin receptor leading to an apparent increase in the interaction of the receptor with fibronectin.
Brown
P J
PJ
Department of Pharmacology, University of Texas Medical School, Houston 77225.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005353:Fibronectins / Q000378:metabolism*; D007700:Kinetics; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D005353:Fibronectins; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D013755:Tetradecanoylphorbol Acetate