Fc receptor-mediated binding and ingestion of immunoglobulin G-coated erythrocytes by the epithelium of the posterior ciliary processes: an in-vitro study.
In the present in vitro study of intact ciliary processes, we observed, under differential interference microscopy, IgG inhibitable selective epithelial adherence and internalization of IgG-coated erythrocytes (E-IgG). Unequivocal evidence of ligand ingestion was obtained with a fluorescence technique which employed glutaraldehyde-fixed opsonized erythrocytes. Neither adherence nor internalization were seen when uncoated erythrocytes, IgM, or F (ab')2-coated erythrocytes were substituted for E-IgG in the incubation media. These in-vitro studies demonstrated that the cell membranes of this epithelium exhibit a specific receptor for IgG which may clear the aqueous of IgG aggregates and be associated with the exocytosis of soluble mediators of inflammation.
Siegelman
J
J
Veterans Administration Medical Center, Northport, NY 11768.
Peress
N S
NS
D000268:Adhesiveness; D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology; D002924:Ciliary Body / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D066298:In Vitro Techniques; D010587:Phagocytosis; D011817:Rabbits; D011961:Receptors, Fc / Q000276:immunology; D017452:Receptors, IgG; D013997:Time Factors
D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG