Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.
A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.
Winans
S C
SC
Elledge
S J
SJ
Krueger
J H
JH
Walker
G C
GC
D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D011938:Rec A Recombinases