Dopamine-stimulated fucosylation of brain proteins in vitro is not inhibited by puromycin.
In slices of rat hippocampus, the influence of puromycin (0.5 mM) on dopamine (0.5 mM)-stimulated L-fucose incorporation into glycoproteins was studied. Puromycin (15 min, 2 h and 4 h pretreatment) inhibited protein synthesis by some -70% (estimated by the in vitro incorporation rate of L-leucine), whilst L-fucose incorporation into rat hippocampal glycoproteins was only inhibited about -20% (4 h pretreatment) compared to control values (100%). Under these experimental conditions, the dopamine-induced increase in both the L-fucose incorporation into glycoproteins and the activity of fucokinase was not affected by puromycin. These data suggest that these short-lasting dopamine actions do not depend on protein synthesis.
Lössner
B
B
Linke
I
I
Eppendorfer
B
B
Jork
R
R
D000818:Animals; D004298:Dopamine / Q000494:pharmacology*; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011691:Puromycin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D006023:Glycoproteins; D009419:Nerve Tissue Proteins; D005643:Fucose; D011691:Puromycin; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020153:fucokinase; D004298:Dopamine