Regulation of growth and differentiation of pre-activated B lymphocytes.
The differential effects of H-2 IAk-specific T helper cells, their soluble factors and Sepharose-coupled anti-mu antibodies on the growth and differentiation of pre-activated B cells were studied. It was found that the prolonged growth of pre-activated B cells required activation signals from major histocompatibility complex (MHC)-restricted T helper cells or Sepharose-coupled anti-mu antibodies. The MHC-restricted T helper cells induced both prolonged growth and differentiation of activated B cells. Anti-mu antibodies together with T helper cell-derived soluble factors induced prolonged growth of activated B cells, but no differentiation into Ig secretion was detected. The inhibition of Ig secretion in anti-mu cultures could be overcome to some extent by either MHC-restricted T helper cells or lipopolysaccharide together with soluble factors. It was also observed that T helper cell interactions were needed for long-term in vitro culture of pre-activated B lymphocytes.
Rajasekar
R
R
Department of Immunology, University of Uppsala, Sweden.
Andersson
J
J
Leanderson
T
T
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D002454:Cell Differentiation; D007075:Immunoglobulin M / Q000276:immunology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D008222:Lymphokines