title: DNA sequence analysis of the Olir2-76 and Ossr1-92 alleles of the Oli-2 region of the yeast Saccharomyces cerevisiae. Analysis of related amino-acid substitutions and protein-antibiotic interaction.
abstract: Petite deletion mapping helped to generate a fine-structure genetic map of the Oli-2 region of the mitochondrial genome of Saccharomyces cerevisiae. Here we report the DNA sequence analysis of the Oli-2 region from two drug-resistant alleles (Olir2-76 and Ossr1-92) which are located in the gene for subunit-6 of mitochondrial ATPase, in agreement with their genetic locations on the mitochondrial genome. An analysis of the corresponding amino-acid substitutions is also presented in the context of protein-antibiotic interactions.
authors.affiliation: Department of Chemistry and Molecular Sciences, University of Warwick, Coventry, U.K.
authors.forename: M K
authors.initials: MK
authors.lastname: Ray
authors.forename: I F
authors.initials: IF
authors.lastname: Connerton
authors.forename: D E
authors.initials: DE
authors.lastname: Griffiths
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D004351:Drug Resistance / Q000235:genetics; D018942:Macrolides; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009840:Oligomycins / Q000378:metabolism / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D018942:Macrolides; D008565:Membrane Proteins; D009840:Oligomycins; C008717:ossamycin; D000251:Adenosine Triphosphatases