Distribution of cells bearing the Tac antigen during ontogeny of human lymphoid tissue.
The monoclonal antibody anti-Tac, which binds to the interleukin 2 (IL 2) receptor, was used to identify this antigen in human fetal and adult lymphoid tissue. Liver, spleen, thymus, lymph node, and peripheral blood were examined for Tac-positive cells with the use of frozen sections or cytocentrifuge preparations. The results show that cells in the fetal and neonatal thymus express the Tac antigen; these cells are predominantly located in the medulla. The liver and spleen of both fetus and adult exhibit very few Tac-positive cells. Double staining demonstrates that cells bearing the Tac-antigen stain with Leu-4, an anti-T cell antibody. In adult lymph node tissue, the Tac-bearing cells are predominantly distributed in the interfollicular area, with positive cells also present in the germinal center and mantle zone. The Tac antigen is present on both T and B cells. Few Tac-positive cells are present in the circulating peripheral blood.
Hofman
F M
FM
Modlin
R L
RL
Bhoopat
L
L
Taylor
C R
CR
D000328:Adult; D000375:Aging; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology* / Q000502:physiology; D002454:Cell Differentiation; D005333:Fetus; D006801:Humans; D007231:Infant, Newborn; D008099:Liver; D008221:Lymphoid Tissue / Q000166:cytology* / Q000254:growth & development / Q000276:immunology; D013154:Spleen; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D013950:Thymus Gland; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7