title: Monoclonal antibody internalization and degradation during modulation of the CD3/T-cell receptor complex.
abstract: Although it is well known that the CD3/T-cell receptor (TCR) complex modulates from the surface of T cells upon exposure to monoclonal antibodies (mAb) directed against it, the fate of bound mAb has not been yet elucidated. We therefore perform direct binding experiments of 125I-labeled mAb against CD3 or TCR to investigate their fate in Jurkat T cells. We demonstrated that all mAb were progressively internalized and degraded in Jurkat T cells and that this degradation was inhibited by chloroquine, an inhibitor of lysosomal degradation enzymes. The sequestration of anti-CD3 mAb in acid compartments was furthermore shown using cytofluorometry. All together our results show that antibodies against CD3 or against TCR follow the same endocytic pathway.
authors.affiliation: INSERM U210, Faculté de Médecine, Nice, France.
authors.forename: L
authors.initials: L
authors.lastname: Schaffar
authors.forename: A
authors.initials: A
authors.lastname: Dallanegra
authors.forename: J P
authors.initials: JP
authors.lastname: Breittmayer
authors.forename: S
authors.initials: S
authors.lastname: Carrel
authors.forename: M
authors.initials: M
authors.lastname: Fehlmann
mesh_terms: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D017252:CD3 Complex; D002451:Cell Compartmentation; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D004705:Endocytosis; D006801:Humans; D007152:Immunologic Capping; D008024:Ligands; D008970:Molecular Weight; D011940:Receptor Aggregation; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D008024:Ligands; D011948:Receptors, Antigen, T-Cell; D002738:Chloroquine