[Changes in purine metabolism in the macrophages of mice exposed to a new synthetic analog of muramyl dipeptide].
It has been established that N-acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutamine (GMDP), a new synthetic analog of muramyl dipeptide, while incubated in vitro with macrophages essentially inhibits 5'-nucleotidase (5-N) activity without any influence on the activity of adenosine deaminase in these cells. The maximal effect was recorded 24 h after co-incubation. As 0.01 = 1 microgram/ml concentration of GMDP was added, the enzyme activity gradually decreased to minimum. L-D-isomer of GMDP was shown to affect 5-N activity whereas the effect of its analog with a double peptide chain GM (DP)2 was found to be less. Inhibition of 5-N activity may be one of the mechanisms by which macrophages are activated under the influence of GMDP.
Umanskiĭ
V Iu
VIu
Tarakhovskiĭ
A M
AM
Andronova
T M
TM
D015720:5'-Nucleotidase; D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000243:Adenosine Deaminase / Q000378:metabolism; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009708:Nucleotidases / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D013997:Time Factors
D000276:Adjuvants, Immunologic; D011687:Purines; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C023868:N-acetyl-beta-glucosaminyl-N-acetylmuramyl-alanylisoglutamine; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase
Izmenenie purinovogo metabolizma v makrofagakh mysheĭ pod vliianiem novogo sinteticheskogo analoga muramildipetida.