Adenosine uptake in pyrimidine 5'-nucleotidase deficient human erythrocytes via a high affinity transport system.
Using a pulse-labeling technique, 14C-adenosine uptake into pyrimidine 5'-nucleotidase (P5N) deficient erythrocytes (RBC) was found to be impaired. The Lineweaver-Burk plot showed Km values of 2.0 X 10(-3) mM and 0.2 X 10(-3) mM for normal RBC and P5N deficient RBC, respectively. These results indicate that P5N is one of regulators of the adenosine transport system and/or is associated with adenosine carrier protein.
Kagimoto
T
T
Tomino
S
S
Takatsuki
K
K
D015720:5'-Nucleotidase; D000241:Adenosine / Q000097:blood*; D001692:Biological Transport; D004912:Erythrocytes / Q000378:metabolism*; D006579:Heterozygote; D006801:Humans; D007700:Kinetics; D009708:Nucleotidases / Q000172:deficiency*; D013103:Spherocytosis, Hereditary / Q000097:blood
D009708:Nucleotidases; D015720:5'-Nucleotidase; D000241:Adenosine