title: Cloning and sequence analysis of a cDNA encoding the beta-subunit of mouse beta-hexosaminidase.
abstract: A cDNA encoding the prepro-beta-polypeptide of mouse beta-hexosaminidase (Hex) was isolated from a mouse lymphoblast cDNA library. The cDNA contains an open reading frame corresponding to a polypeptide of 536 amino acids which shows 74% homology with the human prepro-beta-polypeptide. An examination of the amino acid sequence identifies a putative signal peptide and five possible glycosylation sites, two of which are identical to the confirmed glycosylation sites of the human beta-chain. The amino acid sequence also shows a structurally similar though not identical site for internal cleavage responsible for the generation of mature beta a- and beta b-polypeptides.
authors.affiliation: Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Bapat
authors.forename: M
authors.initials: M
authors.lastname: Ethier
authors.forename: K
authors.initials: K
authors.lastname: Neote
authors.forename: D
authors.initials: D
authors.lastname: Mahuran
authors.forename: R A
authors.initials: RA
authors.lastname: Gravel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005796:Genes; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics*
chemical_list: D046911:Macromolecular Substances; D004247:DNA; D001619:beta-N-Acetylhexosaminidases