Dot-blot assays and their use as a direct antigen-binding method to screen monoclonal antibodies to 1,4-beta- and 1,3-beta-glucan synthases.
A rapid method has been developed to assay beta-glucan synthases spotted on a nitrocellulose sheet. The sensitivity of this method allows screening of hybridoma-making monoclonal antibodies in a direct antigen-binding assay by measurement of the activity of the enzymes retained by the antibodies previously fixed on nitrocellulose.
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Laboratoire de Différenciation Fongique, UA CNRS 1127, Université Claude Bernard Lyon, Villeurbanne, France.
Grange
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Fevre
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D029681:Arabidopsis Proteins; D005964:Glucosyltransferases / Q000276:immunology*; D015151:Immunoblotting / Q000379:methods; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009868:Oomycetes / Q000201:enzymology; D029702:Schizosaccharomyces pombe Proteins
D000911:Antibodies, Monoclonal; D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D029702:Schizosaccharomyces pombe Proteins; D005964:Glucosyltransferases; C457769:PRC1 protein, Arabidopsis; C028634:cellulose synthase (UDP-forming); C020570:1,3-beta-glucan synthase