Mechanism of T cell-derived helper factor production upon stimulation with pokeweed mitogen in humans.
T cell-derived helper factors can cause activated B cells to proliferate and differentiate into immunoglobulin (Ig)-secreting cells. In the pokeweed mitogen (PWM)-driven Ig production system, the cell-cell interaction necessary for the production of the helper factors was analysed. T cells were pulsed with PWM with or without autologous monocytes. The T cells were then isolated, and incubated for 48 h. Supernatants were tested for their ability to promote differentiation of activated B cells. We found that cell-cell contact between T cells and monocytes for the first 3 h of culture is required to produce the T cell-derived helper factors upon stimulation with PWM. We next examined which T cell subsets are involved in the production of helper factors and which surface molecules either on T cells or on monocytes are responsible for the cell-cell interaction to produce helper factors. A series of monoclonal antibodies were added to T cell subsets with monocytes during the PWM-pulsed period. Although T4+ and T8+ cells could produce almost equal amounts of helper factor activity upon PWM-stimulation, the interactions between T3 and T4 antigens on T4+ cells and Ia-like antigens on monocytes and those between T3 and T8 antigens on T8+ cells and HLA class I antigens on monocytes are respectively essential for the production of T4+ cell-derived and T8+ cell-derived helper factors.
Suzuki
N
N
Department of Internal Medicine, Shimane Medical University, Japan.
Sakane
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000957:Antigens, Viral, Tumor / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002450:Cell Communication; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D009000:Monocytes / Q000276:immunology; D011043:Pokeweed Mitogens; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000957:Antigens, Viral, Tumor; D006680:HLA Antigens; D007375:Interleukin-1; D011043:Pokeweed Mitogens