title: Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme.
abstract: A mouse lactate dehydrogenase-A cDNA was isolated and it was shown to contain the 393bp of the protein-coding sequence and 488bp of the 3' untranslated region. The amino acid sequence deduced from its open reading frame provided independent evidence for the sequence of residues 201-331 of mouse LDH-A subunit (muscle). This cDNA clone was used as a probe to isolate a mouse genomic clone containing a truncated, processed LDH-A pseudogene. This pseudogene showed 81.6% homology at 713 positions compared with the LDH-A cDNA sequence. The divergence of this pseudogene was estimated to have occurred 39 million years ago.
authors.forename: K
authors.initials: K
authors.lastname: Akai
authors.forename: K
authors.initials: K
authors.lastname: Yagi
authors.forename: H F
authors.initials: HF
authors.lastname: Tiano
authors.forename: Y C
authors.initials: YC
authors.lastname: Pan
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: K
authors.initials: K
authors.lastname: Fong
authors.forename: R A
authors.initials: RA
authors.lastname: Jungmann
authors.forename: S S
authors.initials: SS
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D005796:Genes; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D051379:Mice
chemical_list: D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase; D004262:DNA Restriction Enzymes