title: Gonadotrophin-stimulated cyclic AMP production by granulosa cells from Booroola x Romney ewes with and without a fecundity gene.
abstract: The influence of follicular size and health on FSH and LH stimulation of cAMP production by granulosa cells in vitro was studied in cells from Booroola X Romney ewes, with (F+) and without (++) a fecundity gene. The granulosa cells were obtained 0-48 h after the initiation of luteolysis on Day 10 of the oestrous cycle by cloprostenol. The highest mean amounts of cAMP produced by granulosa cells challenged with FSH or LH were not significantly different between the genotypes. However, they were achieved using granulosa cells from follicles greater than 3-4 mm in diameter in F+ ewes but from follicles greater than 4 mm in diameter in ++ ewes. Follicles may thus attain ovulatory maturity at a smaller diameter in F+ ewes than in ++ ewes. Granulosa cells from most atretic follicles gave a poor cAMP response to FSH or LH, compared to cells from non-atretic follicles. Granulosa cell responsiveness to FSH was independent of the time the cells were recovered after cloprostenol treatment in F+ ewes, but not in ++ ewes. Cellular responsiveness to LH was independent of time for sheep of both genotypes. There was a significant positive relationship for sheep of both genotypes between the level of aromatase activity in granulosa cells and cellular responsiveness to FSH and LH.
authors.forename: K M
authors.initials: KM
authors.lastname: Henderson
authors.forename: L E
authors.initials: LE
authors.lastname: Kieboom
authors.forename: K P
authors.initials: KP
authors.lastname: McNatty
authors.forename: S
authors.initials: S
authors.lastname: Lun
authors.forename: D
authors.initials: D
authors.lastname: Heath
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism; D003008:Cloprostenol / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D005260:Female; D005298:Fertility; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005838:Genotype; D006062:Gonadotropins / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects* / Q000201:enzymology; D007986:Luteinizing Hormone / Q000494:pharmacology; D006080:Ovarian Follicle / Q000033:anatomy & histology; D010060:Ovulation; D012756:Sheep
chemical_list: D006062:Gonadotropins; D003008:Cloprostenol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D001141:Aromatase