The effect of concentration on the antioxidant effectiveness of alpha-tocopherol in lipid peroxidation induced by superoxide free radicals.
Egg yolk phosphatidylcholine liposomes were rapidly oxidized in the presence of chelated iron and a superoxide-generating system. alpha-Tocopherol incorporated in the bilayer was oxidized at the same time. No lipid or alpha-tocopherol oxidation occurred in liposomes composed of dimyristoyl phosphatidylcholine. The antioxidant did not inhibit lipid peroxidation until its concentration reached a critical level, which depended on the effectiveness of the oxidative stress. Beyond this level, peroxidation was inhibited completely and, simultaneously, the rate of oxidation of tocopherol was lowered. The results suggest that the antioxidant efficiency of alpha-tocopherol depends on its ability to react mainly with the chain-initiating or chain-propagating lipid radicals. This, in turn, is closely tied to the tocopherol content of the membrane. Ascorbate inhibited the consumption of alpha-tocopherol, possibly by regenerating its reduced form.
Fukuzawa
K
K
Takase
S
S
Tsukatani
H
H
D000975:Antioxidants; D001205:Ascorbic Acid / Q000494:pharmacology; D005609:Free Radicals; D008051:Lipid Bilayers; D008054:Lipid Peroxides / Q000096:biosynthesis*; D008081:Liposomes / Q000378:metabolism*; D008956:Models, Chemical; D010084:Oxidation-Reduction / Q000187:drug effects; D010713:Phosphatidylcholines / Q000378:metabolism; D013481:Superoxides / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D005609:Free Radicals; D008051:Lipid Bilayers; D008054:Lipid Peroxides; D008081:Liposomes; D010713:Phosphatidylcholines; D013481:Superoxides; D014810:Vitamin E; D001205:Ascorbic Acid