title: A modified photometric quantification of skin surface lipids sampled using the cigarette paper method.
abstract: In severe cases of acne conglobata the use of substances which considerably reduce the sebum excretion rate, such as 13-cis-retinoic acid, is reasonable. To monitor the effect of such preparation a noninvasive and accurate method for measuring skin surface lipids (SSLs) is required. A new modified photometric quantification of SSLs sampled using the cigarette paper method based on reaction with phosphovanilic reagent was introduced. Our overall results indicate that a 3-h SSLs assessment measured photometrically is as accurate as the gravimetric technique; it is reproducible in situations with low and high levels of SSLs.
authors.affiliation: Department of Dermatology, Clinical Center Zagreb, Yugoslavia.
authors.forename: S
authors.initials: S
authors.lastname: Bolanca-Bumber
authors.forename: A
authors.initials: A
authors.lastname: Basta-Juzbasić
authors.forename: A
authors.initials: A
authors.lastname: Kansky
authors.forename: T
authors.initials: T
authors.lastname: Gross
mesh_terms: D000152:Acne Vulgaris / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000032:analysis*; D010209:Paper; D012629:Sebum / Q000032:analysis; D012867:Skin / Q000032:analysis*; D013053:Spectrophotometry
chemical_list: D008055:Lipids