Quinacrine antagonizes the effects of Na,K-ATPase inhibitors on renal prostaglandin E2 release but not their effects on renin secretion.
Previous results have demonstrated that two inhibitors of Na-and-K-activated adenosine triphosphatase (ouabain, vanadate) lead to stimulated prostaglandin E2 release and to inhibited renin secretion in the rat renal cortical slice preparation. It was speculated that stimulation of phospholipase A2 activity accounted for the effect on prostaglandin E2 release. We used the same preparation in the present experiments, and showed that another inhibitor of Na-and-K-activated adenosine triphosphatase (K-free incubation medium) stimulates prostaglandin E2 release and inhibits renin secretion. Quinacrine antagonized the stimulatory effects of ouabain, vanadate, and K-free medium on prostaglandin E2 release (consistent with phospholipase A2 involvement), but did not antagonize their inhibitory effects on renin secretion. Collectively, these observations lend further weight to the argument against a mediatory role of prostaglandin synthesis in the renin secretory process.
Churchill
P C
PC
Churchill
M C
MC
McDonald
F D
FD
D000818:Animals; D015232:Dinoprostone; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000037:antagonists & inhibitors; D011458:Prostaglandins E / Q000378:metabolism*; D011796:Quinacrine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012083:Renin / Q000097:blood*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D014638:Vanadates; D014639:Vanadium / Q000037:antagonists & inhibitors
D011458:Prostaglandins E; D014639:Vanadium; D014638:Vanadates; D010042:Ouabain; D012083:Renin; D000254:Sodium-Potassium-Exchanging ATPase; D011796:Quinacrine; D015232:Dinoprostone