Estrogen-controlled gene expression in tissue culture cells by zearalenone.
In two estrogen-sensitive cell lines, Le42 and MCF-7, the estrogenic potential of the nonsteroidal mycotoxin zearalenone has been investigated. The chloramphenicol acetyltransferase (CAT) gene expression in Le42 cells is induced by zearalenone after transfection with a CAT-gene construct controlled by an estrogen responsive element [(1986) Cell 46, 1053-1061]. In MCF-7 cells zearalenone induces at least 2 exoproteins (52 and 160 kDa) which are estrogen-specific [(1980) Cell 20, 353-362). These data suggest that zearalenone acts by activating the estrogen receptor. Due to the high sensitivity of these cell lines for zearalenone both test systems are proposed as assays for a quantitative estimation of the biological (estrogenic) activity of this widespread mycotoxin.
Mayr
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Institut für Tierernährung, Universität Hohenheim, Stuttgart, FRG.
D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology*; D005796:Genes / Q000187:drug effects*; D014176:Protein Biosynthesis; D011960:Receptors, Estrogen / Q000096:biosynthesis; D012118:Resorcinols / Q000494:pharmacology*; D014162:Transfection; D015025:Zearalenone / Q000494:pharmacology*
D011960:Receptors, Estrogen; D012118:Resorcinols; D004958:Estradiol; D015025:Zearalenone; D015500:Chloramphenicol O-Acetyltransferase