title: Topographical distribution of pro-opiomelanocortin-derived peptides (ACTH/beta-END/alpha-MSH) in the rat median eminence.
abstract: The detailed distribution of adrenocorticotropin (ACTH), beta-endorphin (beta-END) and alpha-melanotropin (alpha-MSH) immunoreactivity was examined in the rat median eminence (ME) and pituitary stalk using light microscopic immunocytochemistry and radioimmunoassay (RIA). Nerve fibers and varicosities immunoreactive for ACTH/beta-END/alpha-MSH had identical distributions in the ME suggesting that they are part of the same arcuate proopiomelanocortin neuronal (POMC) system. The quantitative image analysis of POMC immunoreactive varicosities in the ME indicates no significant differences between the various rostro-caudal segments. In the main (preinfundibular) portion of the ME, a moderate density of immunoreactive elements was located in the lateral part of the internal zone and throughout the postinfundibular ME. Very few scattered varicosities were observed in the neurohemal (external) zone and in the pituitary stalk. By RIA, alpha-MSH is present in a substantially higher concentration than ACTH and beta-END throughout the ME. Knife cuts between the arcuate nucleus and ME indicate that proopiomelanocortin (POMC) fibers enter the ME in its whole rostro-caudal extent. Thus POMC neurons seem to provide innervation of structures in the internal zone but not in the neurohemal/external/zone where the portal capillary system is located. Moreover, the observation that the density of immunoreactive elements is substantially lower in the pituitary stalk than in the ME, suggests that the majority of immunoreactive fibers in the internal zone are not fibers of passage directed towards the neurohypophysis.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Kiss
authors.forename: E
authors.initials: E
authors.lastname: Mezey
authors.forename: M D
authors.initials: MD
authors.lastname: Cassell
authors.forename: T H
authors.initials: TH
authors.lastname: Williams
authors.forename: G P
authors.initials: GP
authors.lastname: Mueller
authors.forename: T L
authors.initials: TL
authors.lastname: O'Donohue
authors.forename: M
authors.initials: M
authors.lastname: Palkovits
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D004723:Endorphins / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008473:Median Eminence / Q000032:analysis / Q000378:metabolism*; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D001615:beta-Endorphin
chemical_list: D004723:Endorphins; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones