title: A temperate phage of Streptomyces azureus.
abstract: A new phage, SAt1, was isolated from soil on Streptomyces azureus ATCC 14921. This phage was able to lysogenize S. azureus. The percentage of lysogenic responses was ca. 10%. Electron microscopic observation showed that this phage belonged to group B of Bradley's morphological classification. The molecular mass of SAt1 DNA was ca. 24 megadaltons. The guanine-plus-cytosine content and the density of SAt1 DNA were ca. 71% and 1.724 g/cm3, respectively. A cleavage map of SAt1 DNA was constructed with restriction endonucleases BanIII, BglII, HindIII, and XbaI. Furthermore, some other characteristics of this phage were investigated.
authors.forename: S
authors.initials: S
authors.lastname: Ogata
authors.forename: H
authors.initials: H
authors.lastname: Suenaga
authors.forename: S
authors.initials: S
authors.lastname: Hayashida
mesh_terms: D001435:Bacteriophages / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis; D008242:Lysogeny; D008854:Microscopy, Electron; D012988:Soil Microbiology; D013045:Species Specificity; D013302:Streptomyces; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes