title: Herpes simplex virus type 2 and human papillomavirus type 16 in cervicitis, dysplasia and invasive cervical carcinoma.
abstract: Sera and biopsies of cervical lesions from 55 women with benign or malignant disease were analyzed for evidence of infection with herpes simplex virus type 2 (HSV-2) or human papillomavirus (HPV). In addition, information regarding known risk factors for cervical cancer was obtained by interview. The sera were tested for HSV-2 antibodies and the biopsies were tested for HPV or HSV DNA sequences by Southern blot hybridization. HSV-2 sequences were detected in 2/13 (15%) invasive neoplasms and in 1/12 (7%) benign lesions. Under non-stringent conditions of hybridization, reactions with HPV DNA were detected in biopsies of 2/17 (12%) inflammatory lesions, 6/12 (50%) intraepithelial neoplasms and 13/20 (65%) invasive neoplasms. All but one of the positive biopsies of invasive cancer, but only 4/11 biopsies of non-invasive lesions, contained HPV-16 DNA as determined by stringent hybridization conditions. Women with cervical cancer possessed the risk factors associated with the disease. Cigarette smoking and the presence of HPV-16 DNA were the most prominent risk factors. No evidence of an interaction between HSV-2 and HPV-16 was found among the cases of invasive cervical cancer.
authors.forename: S S
authors.initials: SS
authors.lastname: Prakash
authors.forename: W C
authors.initials: WC
authors.lastname: Reeves
authors.forename: G R
authors.initials: GR
authors.lastname: Sisson
authors.forename: M
authors.initials: M
authors.lastname: Brenes
authors.forename: J
authors.initials: J
authors.lastname: Godoy
authors.forename: S
authors.initials: S
authors.lastname: Bacchetti
authors.forename: R C
authors.initials: RC
authors.lastname: de Britton
authors.forename: W E
authors.initials: WE
authors.lastname: Rawls
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D001706:Biopsy; D004279:DNA, Viral / Q000032:analysis; D004812:Epidemiologic Methods; D005260:Female; D006801:Humans; D008875:Middle Aged; D010176:Panama; D027383:Papillomaviridae; D011230:Precancerous Conditions / Q000382:microbiology*; D012306:Risk; D012698:Serologic Tests; D018139:Simplexvirus; D002578:Uterine Cervical Dysplasia / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*; D002575:Uterine Cervicitis / Q000382:microbiology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral