Sequences outside of the long terminal repeat determine the lymphomogenic potential of Rous-associated virus type 1.
Recombinant avian leukosis viruses have been constructed from the molecularly cloned DNAs of Rous-associated virus type 1 (RAV-1) and Rous-associated virus type 0(RAV-0). Virus encoded by the cloned RAV-1 DNA induced a high incidence of B-cell lymphoma and a moderate incidence of a variety of other neoplasms. Virus encoded by the cloned RAV-0 DNA did not cause disease. Virus recovered from DNA constructions that encoded the gag, pol, and 5' env sequences of RAV-0 and the 3' env and long terminal repeat sequences of RAV-1 did not cause a high incidence of lymphoma. Rather, these constructed viruses induced a low incidence of a variety of neoplasms. Virus recovered from reconstructed pRAV-1 DNA had the same disease potential as did virus recovered from the parental pRAV-1 DNA. These results indicate that the long terminal repeat sequences of RAV-1 do not confer the potential to induce a high incidence of B-cell lymphoma.
Robinson
H L
HL
Jensen
L
L
Coffin
J M
JM
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000302:isolation & purification; D001402:B-Lymphocytes; D001483:Base Sequence; D002645:Chickens; D004274:DNA, Recombinant; D005814:Genes, Viral; D008223:Lymphoma / Q000235:genetics* / Q000382:microbiology; D014764:Viral Proteins
D004274:DNA, Recombinant; D014764:Viral Proteins