title: Estrogen synthetase stimulation by hemin in human choriocarcinoma cell culture.
abstract: The ability of hemin to stimulate estrogen synthetase (aromatase) in cultured human trophoblast cells and in cellular homogenates was investigated and compared with aromatase stimulation by dibutyryl cAMP [(Bu)2 cAMP]. Cells grown with hemin for 24 h, or homogenates incubated for 45 min with hemin, showed maximal aromatase stimulation (150 to 200% of activities in the absence of hemin) at 25 microM and 0.1 microM, respectively. Aromatase stimulation in culture by 25 microM hemin was observed within 4 h after hemin addition, while (Bu)2 cAMP required more than 6 h. Intracellular heme and porphyrin levels were higher (160 to 185%) in 96 h (Bu)2 cAMP-grown cells than control cells.
authors.forename: F L
authors.initials: FL
authors.lastname: Bellino
authors.forename: R O
authors.initials: RO
authors.lastname: Hussa
mesh_terms: D001141:Aromatase / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000201:enzymology*; D004789:Enzyme Activation; D005260:Female; D006418:Heme / Q000031:analogs & derivatives* / Q000032:analysis; D006427:Hemin / Q000494:pharmacology*; D006801:Humans; D010088:Oxidoreductases / Q000378:metabolism*; D011166:Porphyrins / Q000032:analysis; D011247:Pregnancy; D013997:Time Factors; D014327:Trophoblasts / Q000201:enzymology; D014594:Uterine Neoplasms / Q000201:enzymology*
chemical_list: D011166:Porphyrins; D006418:Heme; D003994:Bucladesine; D006427:Hemin; D010088:Oxidoreductases; D001141:Aromatase