Characterization of the peribacteroid membrane ATPase of lupin root nodules.
Peribacteroid membranes can be isolated in essentially pure form from 20-day lupin root nodules by osmotic shock of the purified membrane enclosed bacteroids. The ATPase (EC 3.6.1.3) associated with this membrane has an acid pH optimum (5.25) and is specific for ATP (Mg-ATP Km = 0.16 mM). The enzyme activity requires magnesium or manganese ions, is slightly stimulated by the cations potassium and rubidium, and is inhibited by vanadate, diethylstilbestrol, N,N'-dicyclohexylcarbodiimide, fluoride, molybdate, and calcium. Molybdate and fluoride sensitivity do not in this case indicate the presence of significant nonspecific phosphatase activity. The ATPase is not inhibited by oligomycin, azide, or the soluble carbodiimide 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. In some respects the lupin peribacteroid membrane ATPase appears to differ from the plasma membrane ATPase of other plants.
Domigan
N M
NM
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Farnden
K J
KJ
Robertson
J G
JG
Monk
B C
BC
D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D002412:Cations; D002462:Cell Membrane / Q000378:metabolism; D007887:Fabaceae; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D008345:Manganese / Q000494:pharmacology; D010944:Plants / Q000201:enzymology* / Q000382:microbiology; D010946:Plants, Medicinal; D012231:Rhizobium / Q000201:enzymology*; D012969:Sodium Fluoride / Q000494:pharmacology; D013379:Substrate Specificity; D014638:Vanadates / Q000494:pharmacology
D002412:Cations; D015636:Magnesium Chloride; D014638:Vanadates; D008345:Manganese; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D012969:Sodium Fluoride; D000251:Adenosine Triphosphatases; D008274:Magnesium