Evidence for IFN-beta heterogeneity in a substrain of Namalwa cells.
A substrain of Namalwa cells, denoted substrain B, was grown in fermentors up to the 100-L scale, and was induced with Sendai virus to produce interferon (IFN). The titer of the crude IFN varied extensively between different batches; part of the variation was caused by a differential expression of IFN-alpha and IFN-beta. More than 80% of the IFN activity was IFN-beta by several criteria. A two-step purification procedure was developed and the resulting preparation had a specific activity of approximately 10(6) U/mg protein. The IFN-beta type was found to be heterogeneous, and could be separated into several components, which probably represented post-translational modifications of one molecule.
Borg
H
H
Nordensson
I
I
Ny
T
T
Lundgren
E
E
D000327:Adsorption; D002051:Burkitt Lymphoma / Q000378:metabolism*; D002460:Cell Line; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004854:Herpesvirus 4, Human; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis* / Q000302:isolation & purification; D007525:Isoelectric Focusing; D010222:Parainfluenza Virus 1, Human / Q000276:immunology
D007370:Interferon Type I