c-erbB activation in ALV-induced erythroblastosis: novel RNA processing and promoter insertion result in expression of an amino-truncated EGF receptor.
ALV-induced erythroblastosis results from the specific interruption of the host oncogene, c-erbB, by the insertion of an intact provirus. Integrated proviruses are oriented in the same transcriptional direction as c-erbB, and expression of truncated c-erbB transcripts is observed. Evidence, including sequence analysis of cDNA clones, indicates that transcription of truncated c-erbB mRNA is initiated in the 5' LTR of the integrated provirus. This transcript is processed through a series of remarkable splicing reactions to yield viral gag and env sequences fused to erbB sequences. These results establish a novel pathway of promoter insertion oncogenesis that stands in contrast to the pathways used in the activation of c-myc in B lymphomas.
Nilsen
T W
TW
Maroney
P A
PA
Goodwin
R G
RG
Rottman
F M
FM
Crittenden
L B
LB
Raines
M A
MA
Kung
H J
HJ
D000818:Animals; D001353:Avian Leukosis / Q000235:genetics* / Q000382:microbiology; D001354:Avian Leukosis Virus / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D004247:DNA; D066246:ErbB Receptors; D015683:Gene Products, gag; D005814:Genes, Viral; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D015683:Gene Products, gag; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D004247:DNA; D066246:ErbB Receptors