Human papillomavirus type 16 recombinant DNA is maintained as an autonomously replicating episome in monkey kidney cells.
Human papillomavirus type 16 (HPV16) DNA cloned in the expression vector pSV2-neo has been shown to be maintained in transfected monkey kidney cells as an autonomously replicating episome at a level of 2 to 10 copies per cell. Integration of pSV2-neo/HPV16 DNA with the host genome occurred in transfected mouse fibroblasts. Neither type of cell appeared to be phenotypically transformed.
Chesters
P M
PM
McCance
D J
DJ
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004261:DNA Replication; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D000882:Haplorhini; D007668:Kidney / Q000382:microbiology; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D014158:Transcription, Genetic; D014162:Transfection
D004274:DNA, Recombinant; D004279:DNA, Viral