Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level.
A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5-bromodeoxyuridine (BUdR) in conjunction with 5-fluorodeoxyuridine (FUdR) and BUdR incorporated into DNA was then detected on Lowicryl-embedded sections by immunogold technique using a monoclonal anti-BUdR antibody. After using this method, chromatin and chromosomes are strongly labelled.
Thiry
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Laboratoire de Cytologie et Histologie animale, Liege, Belgique.
Dombrowicz
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D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000276:immunology*; D002286:Carcinoma, Ehrlich Tumor; D004247:DNA / Q000032:analysis / Q000648:ultrastructure*; D004261:DNA Replication; D005467:Floxuridine; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D005467:Floxuridine; D004247:DNA; D001973:Bromodeoxyuridine