Liquid chromatographic determination of medroxyprogesterone acetate in tablets.
A reverse-phase liquid chromatographic method is described for the assay of medroxyprogesterone acetate in tablets. An octadecylsilane (C18) column with a mobile phase of methanol-0.01M dibasic ammonium phosphate (80 + 20 v/v, pH 7.2 +/- 0.1) and photometric detection at 254 nm separates medroxyprogesterone acetate from excipients. Detector responses were linear to concentrations of medroxyprogesterone acetate over the range 50-150 micrograms/mL (r = 0.999). Mean recovery of medroxyprogesterone acetate added to tablet excipients was 100.8%. Mean assay results were 101.3% (n = 3). The assay results are comparable to those obtained by the compendial liquid chromatographic method.
Fatmi
A A
AA
Reid-Rowell, Inc., Analytical Research and Development, Marietta, GA 30062.
Williams
G V
GV
Hickson
E A
EA
D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D008525:Medroxyprogesterone / Q000031:analogs & derivatives* / Q000032:analysis; D017258:Medroxyprogesterone Acetate; D012997:Solvents; D013607:Tablets
D007202:Indicators and Reagents; D012997:Solvents; D013607:Tablets; D017258:Medroxyprogesterone Acetate; D008525:Medroxyprogesterone