Expression of C gamma 4 T cell receptors and lack of isotype exclusion by dendritic epidermal T cell lines.
Although four murine C gamma gene segments (C gamma 1, 2, 3, and 4) are known to exist, the large majority of expressed gamma-chains have been shown to be of the C gamma 1 isotype and no evidence exists for the expression of more than one receptor by gamma delta TCR-bearing cells. We investigated the nature of the TCR expressed on a number of murine dendritic epidermal T cell-derived cell lines by using both Northern blot and immunoprecipitation analyses. One of these CD3+ cell lines (T195) expresses C gamma 4, V gamma 1, and delta mRNA, and its CD3-associated TCR complex can be precipitated by both anti-C gamma 4 and anti-delta sera, indicating that this receptor is a C gamma 4/delta heterodimer. Furthermore, we show that two cell lines (Y245, Y93) express two distinct TCR gamma-chains, one derived from the C gamma 4 locus, whereas the second gamma-chain is probably derived from the C gamma 2 locus. Together with the previous demonstration of C gamma 1/delta TCR on a number of dendritic epidermal T cell lines (DETC), these results indicate that such DETC are capable of expressing a variety of gamma delta TCR and that, in some DETC, isotype exclusion of gamma-chain expression does not occur.
Koning
F
F
Biological Resources Branch, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Yokoyama
W M
WM
Maloy
W L
WL
Stingl
G
G
McConnell
T J
TJ
Cohen
D I
DI
Shevach
E M
EM
Coligan
J E
JE
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000302:isolation & purification; D000954:Antigens, Surface / Q000235:genetics / Q000302:isolation & purification; D017252:CD3 Complex; D002460:Cell Line; D003713:Dendritic Cells / Q000378:metabolism*; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D007132:Immunoglobulin Isotypes / Q000235:genetics / Q000302:isolation & purification*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000302:isolation & purification; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000302:isolation & purification*; D013601:T-Lymphocytes / Q000378:metabolism*
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D017252:CD3 Complex; D007132:Immunoglobulin Isotypes; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell