The proteins expressed in vivo and in vitro by an orbivirus of the Kemerovo serogroup isolated from Ixodes uriae ticks from St. Abb's Head, Scotland.
The genome of FT363 virus, a member of the Kemerovo serogroup of orbiviruses, was shown to consist of 10 segments of dsRNA. Examination of the intracellular proteins expressed by this virus in Vero cells showed the presence of 9 major and several minor products. Similar results were obtained by translation of the virus dsRNA in a rabbit reticulocyte lysate. Comparison of in vivo and in vitro products by immunoprecipitation using polyclonal FT363 ascitic fluid showed a difference in the numbers of polypeptides precipitated. The significance of these results is discussed.
Eley
S M
SM
Nuttall
P A
PA
Moore
N F
NF
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D005814:Genes, Viral; D018163:Orbivirus / Q000032:analysis* / Q000235:genetics; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000032:analysis*; D013987:Ticks / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis*
D012367:RNA, Viral; D014764:Viral Proteins