title: Influence of dietary lipids on microsomal membranes from chick breast muscle.
abstract: 1. The effect of different dietary fat intake on the lipid composition and fluidity of microsomal membranes as well as in the enzymatic activity of the Ca2+-ATPase from chick breast muscle was investigated. 2. When a standard diet was supplemented with 10% sunflower seed oil, an increase in the relative amounts of unsaturated fatty acids and membrane fluidity and a decrease in the cholesterol content was observed. 3. The presence of 6% cholesterol in the diet does not modify the fatty acid composition and the fluidity of the membrane but increased, in a low extension, the cholesterol content. 4. The provision of the sunflower seed oil-rich diet supplemented with cholesterol just 48 hr before death promoted an increase in the relative amounts of unsaturated fatty acids and cholesterol content whereas the membrane fluidity decreased in a significant extent. 5. Despite that dietary lipids gave rise in some cases to changes in lipid composition and in the physical state of the microsomal membrane, neither the Ca2+ uptake capacity nor the ATPase activity were significantly affected.
authors.affiliation: Departamento de Bioquimica y Biologia Molecular, Universidad de Murcia, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Soler
authors.forename: M J
authors.initials: MJ
authors.lastname: Alejandre
authors.forename: M
authors.initials: M
authors.lastname: Garcia-Gonzalez
authors.forename: J L
authors.initials: JL
authors.lastname: Segovia-Parra
authors.forename: F
authors.initials: F
authors.lastname: Fernandez-Belda
authors.forename: J C
authors.initials: JC
authors.lastname: Gomez-Fernandez
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002645:Chickens; D002791:Cholesterol, Dietary / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002791:Cholesterol, Dietary; D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D000252:Calcium-Transporting ATPases