A new method of inhibiting glycolysis in blood samples.
The maintenance of hydrogen ion concentration in blood samples at pH 5.3-5.9 immediately inhibits glycolysis. This effect is due to the inhibition of all glycolytic enzymes, as shown by measurement of various glycolytic intermediates. At the inhibitory pH at 25 degrees C, the glucose content did not decrease over a period of 8 h, but it did decrease by 1.3 +/- 1.1 mg/dl over 24 h.
Uchida
K
K
Kyoto Medical Science Laboratory Incorporation, Hazukashi Fushimi-ku, Japan.
Matuse
R
R
Toyoda
E
E
Okuda
S
S
Tomita
S
S
D000255:Adenosine Triphosphate / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D001800:Blood Specimen Collection; D004573:Electrolytes / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D006593:Hexokinase / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010732:Phosphofructokinase-1 / Q000097:blood
D001786:Blood Glucose; D004573:Electrolytes; D000255:Adenosine Triphosphate; D006593:Hexokinase; D010732:Phosphofructokinase-1