Partial characterization of the macrophage factor that stimulates fibroblasts to produce collagenase and to degrade collagen.
Rabbit bone marrow-derived macrophages in culture produce and release a soluble factor that activates collagenase secretion and collagen degradation by cultured skin fibroblasts from either rabbit, mouse or human origin. The factor is heat-labile and is inactivated by phenylglyoxal. After gel filtration, it is recovered in both an apparent high-Mr (67000-76000) and a low-Mr (9000-14000) form. Chromatography on cation exchangers suggests two molecular species with different charge properties. These characteristics are compatible with known properties of rabbit interleukin 1.
Huybrechts-Godin
G
G
Peeters-Joris
C
C
Vaes
G
G
D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D007375:Interleukin-1 / Q000502:physiology; D007526:Isoelectric Point; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D003012:Microbial Collagenase / Q000096:biosynthesis*; D008970:Molecular Weight; D015846:Monokines; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology*; D011817:Rabbits
D007375:Interleukin-1; D015846:Monokines; D011506:Proteins; D003094:Collagen; D003012:Microbial Collagenase