Replication forks of Escherichia coli are not the preferred sites for lysogenic integration of bacteriophage Mu.
The question of whether bacteriophage Mu prefers replication forks for lysogenic integration into Escherichia coli chromosomes was tested by using two different systems. In the first, inactivation of genes was scored in synchronized cultures infected by Mu at various times. No increase in the mutation frequency of a gene was found after infection at the time of its replication. In the second, the composition of colonies formed by bacteria lysogenized by Mu was determined; the newly formed lysogens should give rise to mixed colonies (containing lysogenized as well as nonlysogenized bacteria), uniform colonies, or both, depending on the mode of integration. Both types of colonies were found, and the fraction of uniform colonies was proportional to the relative length of the unreplicated segment of an average chromosome in the culture. The results in both systems clearly preclude the possibility that a lysogenizing Mu integrates with high preference at the chromosome replication forks.
Sivan
S
S
Department of Biology, Ben Gurion University of the Negev, Beer Sheva, Israel.
Zaritsky
A
A
Kagan-Zur
V
V
D010583:Bacteriophage mu / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008242:Lysogeny; D009154:Mutation; D013696:Temperature
D004269:DNA, Bacterial