title: Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase-type plasminogen activator.
abstract: Controlled extracellular proteolysis is catalyzed in part by the secretion of plasminogen activators. As a step in the study of the expression of these enzymes in mouse tissues, we have isolated five cDNAs encoding the mouse urokinase-type plasminogen activator from a cDNA library prepared with size-selected mRNA from MSV-transformed 3T3 cells. The longest cDNA insert contains the entire coding region of mouse urokinase, 58 base pairs of the 5' non-coding region, and the entire 3' non-coding region, which is 942 base pairs long. The deduced protein sequence, which starts with a signal peptide of 20 amino acids, shows extensive homology to that of human and porcine urokinase. However, in contrast to these enzymes, mouse urokinase contains no N-glycosylation site. Bacteria harbouring one of the recombinant plasmids synthesize and secrete into their periplasm a protease indistinguishable from mouse urokinase.
authors.forename: D
authors.initials: D
authors.lastname: Belin
authors.forename: J D
authors.initials: JD
authors.lastname: Vassalli
authors.forename: C
authors.initials: C
authors.lastname: Combépine
authors.forename: F
authors.initials: F
authors.lastname: Godeau
authors.forename: Y
authors.initials: Y
authors.lastname: Nagamine
authors.forename: E
authors.initials: E
authors.lastname: Reich
authors.forename: H P
authors.initials: HP
authors.lastname: Kocher
authors.forename: R M
authors.initials: RM
authors.lastname: Duvoisin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D051379:Mice; D009693:Nucleic Acid Hybridization; D010960:Plasminogen Activators / Q000235:genetics*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004247:DNA; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator