Immunohistochemical localization of atrial natriuretic polypeptide (ANP) in human atrial and ventricular myocardiocytes.
To date, there have been few immunohistochemical investigations of atrial natriuretic polypeptide (ANP) in human cardiac tissue, especially the ventricles. In this study, myocardial tissue was obtained from two sources: the bilateral atria and ventricles at autopsy; and biopsy tissues from the right auricle and left ventricle of a patient with myocardial infarction undergoing surgery. These tissues were examined by the avidin-biotin immunoperoxidase technique using three kinds of primary ANP-antibodies. ANP-immunoreactivity was observed in the perinuclear region of myocytes of all tissues examined. The intensity of the reaction was stronger in atrial tissue, weaker in ventricular tissue. In the later tissue, the positive-staining myocytes were not part of the pulse-conducting system. Although the tissues we studied were not obtained from normal hearts, our data demonstrates that ANP-reactivity can be detected in ventricular myocytes outside the pulse-conducting system.
Yamada
H
H
Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
Saito
Y
Y
Mukoyama
M
M
Nakao
K
K
Yasue
H
H
Ban
T
T
Imura
H
H
Sano
Y
Y
D009320:Atrial Natriuretic Factor / Q000032:analysis*; D006325:Heart Atria / Q000032:analysis* / Q000166:cytology; D006352:Heart Ventricles / Q000032:analysis* / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged
D009320:Atrial Natriuretic Factor