title: Molecular cloning and characterization of the cDNA coding for hepatitis B virus surface antigen.
abstract: Hepatitis B virus surface antigen (HBsAg) mRNA has been enriched from a hepatoma cell line (PLC/PRF/5) by specific polysome immunoprecipitation and used for cDNA cloning. A HBsAg cDNA clone was identified by in situ hybridization with a cloned viral probe. It was characterized by restriction endonuclease mapping and DNA sequence analysis. Molecular hybridization of PLC/PRF/5 cellular DNA and RNA to [32P]-labeled HBsAg cDNA revealed the integration of at least six copies of the hepatitis B virus (HBV) DNA into the host genome and expression of three DNA species containing HBsAg-specific sequences. The possible role of HBV in the oncogenesis of primary hepatocellular carcinoma is discussed.
authors.forename: B G
authors.initials: BG
authors.lastname: He
authors.forename: J L
authors.initials: JL
authors.lastname: Melnick
authors.forename: A
authors.initials: A
authors.lastname: Siddiqui
authors.forename: W S
authors.initials: WS
authors.lastname: Robinson
authors.forename: S W
authors.initials: SW
authors.lastname: Law
authors.forename: E C
authors.initials: EC
authors.lastname: Lai
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D005815:Genetic Code; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D008113:Liver Neoplasms; D009857:Oncogenes; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D012333:RNA, Messenger