The Neurospora mitochondrial genome: the region coding for the polycistronic cytochrome oxidase subunit I transcript is preceded by a transfer RNA gene.
We have sequenced a 682 bp fragment of Neurospora crassa mitochondrial DNA to complete the sequence between the gene for cytochrome b and the unassigned reading frame, URF U. The sequence contains a gene for a cysteine tRNA. The 5' end of the 6 kb polycistronic transcript of cytochrome c oxidase subunit 1 is immediately downstream from this tRNA. This shows that also in fungal mitochondria tRNAs can be used as processing signals, whereas palindromic sequences containing double Pst I sites, also present in this region, are not used for processing.
de Vries
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Haima
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Brinker
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de Jonge
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JC
D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV / Q000235:genetics*; D005796:Genes; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics*; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D014158:Transcription, Genetic
D003573:Cytochrome b Group; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D003576:Electron Transport Complex IV