Induction of nitroblue tetrazolium reduction in mouse peritoneal macrophages by tumour promoters and inhibition of the induced nitroblue tetrazolium reduction by some inhibitors.
Two polyacetates, aplysiatoxin and debromoaplysiatoxin, as well as 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein and teleocidin enhance nitroblue tetrazolium (NBT) reduction in mouse peritoneal macrophages in vitro. The ED50 values for NBT reduction of these 5 TPA-type tumor promoters were 4.2 ng/ml for TPA, 36 ng/ml for mezerein, 0.53 ng/ml for teleocidin, 1.5 ng/ml for aplysiatoxin and 108 ng/ml for debromoaplysiatoxin. The NBT reduction induced by the 5 tumor promoters is inhibited by 2 inhibitors of tumor promotion, retinoic acid and dibromoacetophenone. The possibility that tumor promotion by TPA-type tumor promoters involves similar mechanisms such as superoxide anion radicals release in cell membranes is discussed.
Ohkawa
Y
Y
Iwata
K
K
Shibuya
H
H
Inui
N
N
D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D004224:Diterpenes; D005260:Female; D005609:Free Radicals; D008235:Lyngbya Toxins / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology
D000098:Acetophenones; D002273:Carcinogens; D004224:Diterpenes; D005609:Free Radicals; D008235:Lyngbya Toxins; D013729:Terpenes; D013778:Tetrazolium Salts; D013481:Superoxides; C022176:teleocidins; D009580:Nitroblue Tetrazolium; C011309:mezerein; C017179:debromoaplysiatoxin; C006610:aplysiatoxin; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate; C006821:4-bromophenacyl bromide