title: Transplantation of aortic and pulmonary allografts, enhanced viability of endothelial cells by cryopreservation, importance of histocompatibility.
abstract: Fresh heart valve allografts were preserved at 4 degrees C for 14 days, cryopreserved and stored for 63 days, and studied for endothelial viability and antigenicity, in order to obtain some information on the immunobiological status of allografts before transplantation. The surgical technique described by Ross for subcoronary position is preferred and briefly outlined. Four explanted incompatible allografts were studied by light and scanning electron microscopy and immunohistochemistry to assess the immunological reactions and tissue changes that occurred between 9 days and 16 weeks postoperatively. Valve leaflet motion and opening velocities were studied by echocardiography postoperatively to establish a baseline with which to distinguish early leaflet degeneration. Distensibility of the aortic annulus was studied postoperatively by supraaortic angiography to justify one of the goals of reconstruction of the aortic root with allografts. Antibiotic preserved allografts at 4 degrees C showed no viable endothelial cells after 8 days while the cryopreserved allografts demonstrated a high rate of viable endothelial cells capable of expressing surface antigens (HLA class I and II). Although the valve explants showed focal mononuclear cell infiltrations with T-lymphocytes, the allografts healed in place. The "classic" findings of rejection could not therefore be demonstrated. In summary, cryopreserved valve allografts, like the fresh, are antigenic. It is therefore recommended to use compatible valve grafts, when possible, which might be a positive step to improve the functional longevity of valve allografts. Immune response after valve allograft transplantation does not cause acute valvular dysfunction but rather chronic tissue changes which might lead to early degeneration of the allograft. The opening velocities of preserved aortic and pulmonary allografts were normal at 3-4 years postoperatively irrespective of histocompatibility. Echocardiography might be a useful tool to detect early degenerative changes of incompatible valve leaflets. The aortic root is distensible after allograft transplantation.
authors.affiliation: German Heart Center, Berlin-West.
authors.forename: A C
authors.initials: AC
authors.lastname: Yankah
authors.forename: H U
authors.initials: HU
authors.lastname: Wottge
authors.forename: H K
authors.initials: HK
authors.lastname: Muller-Hermelink
authors.forename: A C
authors.initials: AC
authors.lastname: Feller
authors.forename: P
authors.initials: P
authors.lastname: Lange
authors.forename: U
authors.initials: U
authors.lastname: Wessel
authors.forename: H
authors.initials: H
authors.lastname: Dreyer
authors.forename: A
authors.initials: A
authors.lastname: Bernhard
authors.forename: W
authors.initials: W
authors.lastname: Müller-Ruchholtz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001021:Aortic Valve / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D002470:Cell Survival; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation; D004730:Endothelium, Vascular / Q000473:pathology; D006648:Histocompatibility; D006801:Humans; D007223:Infant; D008875:Middle Aged; D009926:Organ Preservation; D011664:Pulmonary Valve / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D013318:Stroke Volume / Q000502:physiology; D014022:Tissue Survival; D009927:Tissue and Organ Procurement; D014181:Transplantation Immunology; D014184:Transplantation, Homologous