Interferon gamma is active on human lymphoblastoid Namalva cells without inducing an antiviral state.
We demonstrate the presence of high affinity receptors specific for interferon-gamma (IFN-gamma) in human lymphoblastoid Namalva cells. The presence of these receptors, whose binding affinity and cross-linking characteristics were not distinguishable from those of the corresponding receptors in sensitive cells, was not consistent with the lack of responsiveness of Namalva cells to IFN-gamma as regards growth inhibition, induction of 2'-5' oligoadenylate synthetase activity and inhibition of virus multiplication. Nevertheless, IFN-gamma enhanced the expression of two genes, HLA class II and c-myc. Although the mechanism of these IFN-gamma-mediated modifications is not understood, these results provide evidence that the IFN-gamma receptors present in Namalva cells are functional.
der Stepani
I
I
Unité 196 INSERM, Institut Curie, Paris, France.
Stefanos
S
S
Lando
D
D
Falcoff
R
R
Wietzerbin
J
J
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D003432:Cross-Linking Reagents; D004790:Enzyme Induction / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006681:HLA-D Antigens / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000502:physiology*; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D014762:Viral Interference
D003432:Cross-Linking Reagents; D006681:HLA-D Antigens; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase