Acidic fibroblast growth factor stimulates opsin levels in retinal photoreceptor cells in vitro.
It is demonstrated that newborn rat retinal photoreceptor cells can differentiate in monolayer culture, and synthesize de novo photoreceptor-specific proteins such as opsin. When maintained in serum supplemented medium on a laminin substrate, these cells survive for up to 3 weeks. The addition of acidic fibroblast growth factor stimulates an increase in the levels of opsin of 5-10-fold control values, and prolongs cell survival by up to 6 days.
Hicks
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Unité de Recherches Gérontologiques, INSERM U118-CNRS U A630, Paris, France.
Courtois
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Y
D000818:Animals; D002478:Cells, Cultured; D005136:Eye Proteins / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D007700:Kinetics; D010786:Photoreceptor Cells / Q000187:drug effects / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D012168:Retinal Pigments / Q000378:metabolism*; D017299:Rod Opsins
D005136:Eye Proteins; D012168:Retinal Pigments; D017299:Rod Opsins; D005346:Fibroblast Growth Factors