Mapping of cellular protein-binding sites on the products of early-region 1A of human adenovirus type 5.
The binding sites for the 300-, 107-, and 105-kilodalton cellular proteins which associate with human adenovirus type 5 E1A products were studied with E1A deletion mutants. All appeared to bind to the amino-terminal half of E1A products in regions necessary for oncogenic transformation. These results suggest that these cellular species may be important for the biological activity of E1A products.
Egan
C
C
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Jelsma
T N
TN
Howe
J A
JA
Bayley
S T
ST
Ferguson
B
B
Branton
P E
PE
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics*; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D001665:Binding Sites; D002872:Chromosome Deletion; D005796:Genes; D005814:Genes, Viral; D006801:Humans; D007624:KB Cells; D008970:Molecular Weight; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding
D017456:Adenovirus Early Proteins; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral