Spin state studies on cytochrome P-450 in liver microsomes from obese and diabetic animals.
The spin state of liver microsomal cytochrome P-450 from obese mice and streptozotocin-diabetic mice and rats has been studied both by the temperature and the type I substrates-induced spectral changes. The high spin cytochrome P-450 is significantly decreased in these animals. Moreover absolute spectra indicate that low spin cytochrome P-450 is stabilized in streptozotocin induced-diabetic animals. Thus the physiopathological state may modify the in vivo spin state of cytochrome P-450 and modifications of the microsomal fatty acid composition might contribute to these changes.
Rouer
E
E
Delaforge
M
M
Rocchiccioli
F
F
Gibson
G G
GG
Leroux
J P
JP
D000818:Animals; D003577:Cytochrome P-450 Enzyme System; D003920:Diabetes Mellitus / Q000201:enzymology*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004578:Electron Spin Resonance Spectroscopy; D005227:Fatty Acids / Q000032:analysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D008862:Microsomes, Liver / Q000201:enzymology*; D009765:Obesity; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry
D005227:Fatty Acids; D003577:Cytochrome P-450 Enzyme System