title: Establishment and characterization of an Epstein-Barr virus nuclear antigen-negative hairy cell line.
abstract: Continuous cell lines have been established from spleen cells of patients with confirmed hairy cell leukemia (HCL). One cell line, HCL-Z1, lacks Epstein-Barr virus nuclear antigen (EBNA), grows attached to the substratum and retains typical features of hairy cells as revealed by transmission and scanning electron microscopy. HCL-Z1 differs morphologically from the three other EBNA-positive lymphoblastoid cell lines obtained (HCL-Z2, HCL-Z3, HCL-Z4) as well as from normal spleen cells or lymphocytes. The three lymphoblastoid cell lines derived from HCL patients show similar surface features as a line from a myeloma patient. Therefore, not all cell lines derived from HCL patients may be considered as representative of the patients leukemia cells.
authors.forename: A B
authors.initials: AB
authors.lastname: Lang
authors.forename: B F
authors.initials: BF
authors.lastname: Odermatt
authors.forename: D R
authors.initials: DR
authors.lastname: Gut
authors.forename: J R
authors.initials: JR
authors.lastname: Rüttner
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000382:microbiology / Q000473:pathology*; D008854:Microscopy, Electron; D013154:Spleen / Q000473:pathology
chemical_list: D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens