Evidence for the existence of multiple forms of choline (ethanolamine) kinase in rat tissues.
In order to characterize the form of choline kinase in rat tissues, both electrophoretic and gel chromatographic patterns of choline kinase activity were compared in the liver, kidney, lung, whole intestine and carbon tetrachloride-induced liver cytosols. Kinetic parameters of the reaction were also compared for the main forms of choline kinase protein from these tissues. The overall results suggested strongly that choline kinase does not exist in one particular active form but exists in multiple forms in rat tissues. In the study present here, the electrophoretic patterns of both choline kinase and ethanolamine kinase activities were compared in rat liver, kidney, lung and intestinal cytosols. The results strongly supported the view that both kinase activities are represented on the same enzyme protein(s) in each of the rat tissues examined.
Ishidate
K
K
Iida
K
K
Tadokoro
K
K
Nakazawa
Y
Y
D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D002797:Choline Kinase / Q000302:isolation & purification*; D003600:Cytosol / Q000201:enzymology; D004790:Enzyme Induction / Q000187:drug effects; D007422:Intestines / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008168:Lung / Q000201:enzymology*; D010770:Phosphotransferases / Q000302:isolation & purification*; D017853:Phosphotransferases (Alcohol Group Acceptor); D051381:Rats
D002251:Carbon Tetrachloride; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); D002797:Choline Kinase; C020125:ethanolamine kinase