Expression of the c-sis gene and secretion of a platelet-derived growth factor-like protein by simian virus 40-transformed BHK cells.
SV40-transformed BHK cells were shown to express two transcripts, of 3.5 kb and 2.0 kb, that hybridised to a human c-sis probe. Antibodies directed against human PDGF specifically recognized a 31 kDa protein in SV40/BHK cell conditioned medium, which upon reduction was split into 16 kDa species. Unfractionated conditioned medium and one of two growth factors isolated from SV40/BHK cells competed with 125I-PDGF for binding to its receptor. The present communication thus provides compelling evidence that an SV40/BHK cell-derived growth factor is a hamster equivalent to human PDGF.
Betsholtz
C
C
Bywater
M
M
Westermark
B
B
Bürk
R R
RR
Heldin
C H
CH
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D005786:Gene Expression Regulation; D005796:Genes; D006133:Growth Substances / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D013539:Simian virus 40
D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor