The effect of delta 1tetrahydrocannabinol on luteinizing hormone release in castrated and hypothalamic deafferented male rats.
delta 1Tetrahydrocannabinol (THC) acutely suppresses tonic serum luteinizing hormone (LH) and prolactin levels in adult male rats. The exact site of its action has not been identified. We have performed complete hypothalamic deafferentation (CHD), which disrupts the medial basal hypothalamus (MBH) from the rest of the CNS, but did not abolish the ability of THC to suppress hypothalamic-pituitary responses in gonadectomized male rats. This was shown by the equal reduction in serum levels of LH and prolactin in non-deafferented (ND) and CHD animals. These results indicate that THC is able to act inside the MBH and that the MBH-pituitary axis remains responsive to its inhibitory effect despite interruption of the neural connections between the MBH and extrahypothalamic areas. However, the corticotropin releasing factor neurons in the MBH appear functionally impaired as a result of the transection and become unresponsive to the normally produced THC stimulation. Different patterns of action seem to govern the various hypophyseal hormones controlled by the hypothalamus, suggesting that the release of LH releasing hormone and prolactin inhibiting factor might be maintained by the activity of neurons surviving inside the island.
Puder
M
M
Nir
I
I
Siegel
R A
RA
Weidenfeld
J
J
Ayalon
D
D
D000344:Afferent Pathways / Q000502:physiology*; D000818:Animals; D002369:Castration; D013759:Dronabinol / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology*; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D008297:Male; D011388:Prolactin / Q000097:blood / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D013759:Dronabinol; D011388:Prolactin; D007986:Luteinizing Hormone