title: Common features of polyomavirus mutants selected on PCC4 embryonal carcinoma cells.
abstract: The genomic rearrangements of six polyomavirus mutants selected on PCC4 embryonal carcinoma cells have been compared and their common characteristics pointed out. All mutants show a duplication which includes at least the adenovirus type 5 (Ad5) E1A-like enhancer core sequence plus a deletion of variable size and location. The presence of the second enhancer core sequence, the SV40-like enhancer, is not required for expression of the PyEC PCC4 phenotype. Two of these mutants are also able to express polyomavirus T antigen on F9 and LT1 cells. Multiadaptation seems to require the duplication of the Ad5 E1A-like core sequence, the maintenance of the SV40-like core sequence and a local change in DNA stability.
authors.forename: F
authors.initials: F
authors.lastname: Melin
authors.forename: H
authors.initials: H
authors.lastname: Pinon
authors.forename: C
authors.initials: C
authors.lastname: Reiss
authors.forename: C
authors.initials: C
authors.lastname: Kress
authors.forename: N
authors.initials: N
authors.lastname: Montreau
authors.forename: D
authors.initials: D
authors.lastname: Blangy
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D051379:Mice; D009154:Mutation; D011120:Polyomavirus / Q000235:genetics*; D013724:Teratoma / Q000382:microbiology*
chemical_list: D004262:DNA Restriction Enzymes