title: Effect of propranolol upon protein and proteolytic synthesis activity in hypertrophic myocardium.
abstract: The aim of the study was to investigate the effect of propranolol upon protein synthesis and degradation processes in cell-free subfractions of rat myocardium in experimental cardiac hypertrophy induced by aortic stenosis. It was found that hypertrophy stimulates incorporation of 3H-amino acids by the postmitochondrial supernatant (PMS) by 17 +/- 4% (mean +/- SE). Propranolol 10 6M inhibited protein synthesis in the control and experimental groups by 37 +/- 5% and 34 +/- 7%, respectively. At a concentration of 5 X 10 6M, propranolol-induced inhibition in each group was 74 +/- 4% and 58 +/- 5%, respectively. The activity of neutral proteases in PMS of hypertrophic hearts increased up to 255 +/- 29 IU in comparison to 154 +/- 10 IU in controls; in the cytosol it was unchanged. Propranolol had no effect upon the activity of proteases in both fractions. Activity of calcium-activated neutral proteases (CANP) remained at the same level in control and hypertrophic hearts and was unaffected by propranolol.
authors.affiliation: Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Kwiatkowska-Patzer
authors.forename: T
authors.initials: T
authors.lastname: Zalewska
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D006332:Cardiomegaly / Q000201:enzymology*; D002474:Cell-Free System; D005260:Female; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D011433:Propranolol / Q000494:pharmacology*; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011433:Propranolol; D010447:Peptide Hydrolases; D002118:Calcium