[Effect of retinoic acid on the differentiation and growth of murine mastocyte precursors].
In cultures of normal mouse hematopoietic cells containing Interleukin-3 develop cells with many features of mast cells. These cells seem heterogeneous with respect to morphological and biochemical examination. Nevertheless, most of the cells show many granules and a low ability to self-renew. In the present report we describe the development of a blastic cell population, termed mastoblasts, when normal mouse hematopoietic cells are exposed continuously to retinoic acid (RA: 10(-6) to 10(-5) M/l). Using H*3-thymidine incorporation, cell cycle measurement and protein content by flow cytometry, transmission and scanning electron microscopy, we show that these cells seem to be of mast cell lineage but with a high self-renewing capability. So, RA is able to inhibit mast cell differentiation and to provide us a "mastoblastic" population which could be used as a model to study mast cell differentiation.
Arock
M
M
Laboratoire d'Hématologie, Faculté de Pharmacie, Paris.
Nafziger
J
J
Gorenflot
A
A
Guillosson
J J
JJ
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D008407:Mast Cells / Q000166:cytology* / Q000254:growth & development; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin
Effet de l'acide rétinoïque sur la différenciation et la croissance des précurseurs mastocytaires murins.