Molecular tools for the mapping of the human genome.
The rapid progress in molecular cloning and DNA analysis techniques, together with the use of cloned DNA probes from specific chromosomes of the human genome might allow localisation and ultimately identification of genes defined by single mutations. The discrepancy between genetic dimensions expressed in centimorgans, each corresponding to millions of base pairs and distances easily accessible by molecular techniques amounting to at the most hundreds of kilobase pairs may be bridged with some new cloning techniques partially developed at the European Molecular Biology Laboratory. These techniques were designed to allow rapid cloning and analysis of large regions of mammalian genomes.
Lehrach
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Frischauf
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Philipson
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D000818:Animals; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D008677:Metaphase; D051379:Mice; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D005819:Genetic Markers; D004262:DNA Restriction Enzymes