title: Separation of enzymically active bovine cytochrome c oxidase monomers and dimers by high performance liquid chromatography.
abstract: The aggregation state of two types of bovine heart cytochrome c oxidase preparations in the presence of laurylmaltoside was investigated by high performance liquid chromatography in two buffers of ionic strengths of 388 mM and 45 mM, respectively. At high ionic strength, it was found that the Fowler cytochrome c oxidase preparation was monomeric (Mr = 2 X 10(5)), while monomers and dimers (2 X aa3, Mr = 4 X 10(5)) could be isolated from the Yonetani preparation. Under these conditions there was no rapid equilibrium between the two forms. Covalent cytochrome c oxidase-cytochrome c complexes were largely dimeric, and addition of ascorbate and cytochrome c to the oxidase also promoted dimerization. At low ionic strength (I = 45 mM) in the presence of laurylmaltoside the oxidase and the covalent complex with cytochrome c were largely monomeric. In the steady-state oxidation of ferrous horse heart cytochrome c, the monomeric enzyme displayed biphasic kinetics at I = 45 mM. This suggests that the presence of high- and low-affinity reactions is an intrinsic property of the cytochrome c oxidase monomer.
authors.forename: T B
authors.initials: TB
authors.lastname: Hakvoort
authors.forename: K M
authors.initials: KM
authors.lastname: Sinjorgo
authors.forename: B F
authors.initials: BF
authors.lastname: Van Gelder
authors.forename: A O
authors.initials: AO
authors.lastname: Muijsers
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003574:Cytochrome c Group / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology; D009994:Osmolar Concentration; D013053:Spectrophotometry
chemical_list: D003574:Cytochrome c Group; D046911:Macromolecular Substances; D003576:Electron Transport Complex IV