Peculiarities of the B to A transition of the lambda phage regulatory site OR3 and of its fragment.
Conformations of the synthetic deoxyoligonucleotide 17 base pairs long, which is an OR3 operator of lambda phage, and of its 9-b.p. fragment were studied by the circular dichroism method (CD). The regions of stability of the double-stranded state were determined for these duplexes. A comparison of the CD spectra for these oligonucleotides with the CD for a lengthy DNA showed the conformation of these short DNA pieces to belong to the B-family. A cooperative change in the CD spectra is observed in trifluoroethanol (TFE) solutions at a TFE concentration specific for each oligonucleotide, which is supposed to stem from a B to A transition. The length of the fragment was found to affect the ability for the B-A transition. The transition into the A form is hindered by 13% TFE for the short 9-nucleotide in comparison with the 17-nucleotide. We suggest that this is due to the B form stabilization by terminal base pairs (B-phility of the ends).
Ivanov
V I
VI
Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Minchenkova
L E
LE
Schyolkina
A K
AK
Chernov
B K
BK
Yartsev
A P
AP
Kirpichnikov
M P
MP
D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002942:Circular Dichroism; D004279:DNA, Viral; D009690:Nucleic Acid Conformation; D009875:Operator Regions, Genetic; D013816:Thermodynamics; D014270:Trifluoroethanol
D004279:DNA, Viral; D014270:Trifluoroethanol