Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.
Monoclonal antibodies (K3-2F2 and K3-4C8) raised against hepatitis A virus were used to develop a solid-phase radioimmunoassay and enzyme-linked immunosorbent assay for the detection of hepatitis A virus and antibody. Assays with this pair of monoclonal antibodies were compared in parallel with similarly constructed solid-phase radioimmunoassays and enzyme-linked immunosorbent assays in which human polyclonal serum was used. The monoclonal antibody assay proved to be more sensitive for the detection of hepatitis A virus from fecal specimens as well as for anti-hepatitis A virus immunoglobulin G (IgG) and IgM in sera.
Coulepis
A G
AG
Veale
M F
MF
MacGregor
A
A
Kornitschuk
M
M
Gust
I D
ID
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D006506:Hepatitis A / Q000175:diagnosis*; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000032:analysis*; D006507:Hepatovirus / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M