[Effect of an increase in the intracellular concentration of calcium ions on the transmembrane current induced by iontophoretic injection of cAMP into Helix pomatia neurons].
The effect of intracellular Ca2+ concentration increase on transmembrane current induced by cAMP injection into a neuron (cAMP-current) was studied on Helix pomatia neurons under voltage clamp conditions. The elevation of intracellular Ca2+ concentration was produced by iontophoretic injection of Ca2+ into the cytoplasm, generation of action potentials, addition of uncouplers of oxidative phosphorylation into the external solution resulting in Ca2+ efflux from mitochondria. It was found that elevation of the intracellular Ca2+ concentration increases the amplitude of the cAMP-current and in most cases its duration as well. It is suggested that membrane structures responsible for the cAMP-current have two centers of phosphorylation: cAMP-dependent and Ca2+-calmodulin-dependent. Possible role of the studied process in integration of signals on the intraneuronal level is discussed.
Kononenko
N I
NI
Shcherbatko
A D
AD
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002147:Calmodulin / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology*; D004140:Dinitrophenols / Q000494:pharmacology; D005724:Ganglia / Q000187:drug effects*; D006372:Helix, Snails; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology
D002147:Calmodulin; D004140:Dinitrophenols; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D000242:Cyclic AMP; D002118:Calcium; C013289:tetraphenylphosphonium
Vliianie uvelicheniia vnutrikletochnoĭ kontsentratsii ionov kal'tsiia na transmembrannyĭ tok, vyzvannyĭ ionoforeticheskoĭ in''ektsieĭ tsAMF, v neĭronakh vinogradnoĭ ulitki.