title: Delineation of DNA sequences that are important for in vitro transcription from the adenovirus EIIa late promoter.
abstract: Late in infection, transcription of the EIIa gene is initiated primarily at map unit 72 of the adenovirus genome. A cell-free nuclear extract system was used to determine sequence elements important for the function of this late promoter. In such a system, the transcriptional activity of a circular template was found to be much higher (5- to 10-fold) than that of a linear template. The effect of template topology appeared to be dependent on two distal upstream elements with 5' boundaries located near -265 to -223 and -147 to -133 (in relation to the initiation site), since deletions of these regions reduced transcription of the circular template, in a stepwise fashion, to a level similar to that observed with the linear template. Further deletions revealed an element in the -116 region that appeared to be more important for transcription of the circular template (10-fold reduction) than for transcription of the linear template (3-fold reduction). Lastly, deletion of the TACAAA sequence in the -29 region resulted in further reduction in transcription, indicating that this element functions as a promoter in vitro.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
authors.forename: D H
authors.initials: DH
authors.lastname: Huang
authors.forename: R G
authors.initials: RG
authors.lastname: Roeder
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D017456:Adenovirus Early Proteins; D004270:DNA, Circular / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D005814:Genes, Viral; D009690:Nucleic Acid Conformation; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D017456:Adenovirus Early Proteins; D004270:DNA, Circular; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral