Microbore reversed-phase chromatography of proteins with conventional gradient equipment for high-performance liquid chromatography.
Reversed-phase high-performance liquid chromatography with microbore columns (50 X 1.0 mm) was used effectively for the separation and analysis of proteins down to 1 ng at flow-rates of 0.1-0.2 ml/min. With the use of standard low-pressure gradient HPLC equipment, the peak volumes were five times smaller when compared with a conventional column at equal chromatographic efficiencies and analysis time. The sensitivity of detection was further increased by a reduction in solvent peaks, resulting in a 20-fold overall increase.
van der Zee
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Welling
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D000097:Acetonitriles; D000818:Animals; D002256:Carbonic Anhydrases / Q000302:isolation & purification; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D003574:Cytochrome c Group / Q000302:isolation & purification; D006736:Horses; D010047:Ovalbumin / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis*; D012260:Ribonucleases / Q000302:isolation & purification
D000097:Acetonitriles; D003574:Cytochrome c Group; D011506:Proteins; D010047:Ovalbumin; D012260:Ribonucleases; D002256:Carbonic Anhydrases; C032159:acetonitrile