Induction of interleukin-4-dependent IgE synthesis and interleukin-5-dependent eosinophil differentiation by supernatants of a human helper T-cell clone.
The human alloreactive T-cell clone A1 produces interleukin (IL)-4, IL-5, and granulocyte macrophage-colony stimulating factor (GM-CSF), but not IL-2 or interferon-gamma (IFN-gamma), as assessed by probing for mRNA transcripts, immunoassays, or bioassays. Supernatants from clone A1 induced IgE synthesis by normal human peripheral blood mononuclear cells. IL-4 was shown to be necessary and sufficient for the induction of IgE by clone A1 supernatants. In contrast, IgE induction by clone A1 supernatants and recombinant (r) IL-4 was inhibited by IFN-gamma. This suggests that the absence of IFN-gamma from the IL-4-containing A1 supernatants was important for their IgE-inducing ability. Supernatants from clone A1 could also specifically induce the growth of cord blood cell progenitors and their differentiation into eosinophils but not into basophils. rIL-5, but not rIL-4 or rGM-CSF, also induced eosinophil maturation. These data suggest that IL-5 secreted by clone A1 was responsible for its ability to induce eosinophil differentiation. The implications of the concomitant production of IL-4 and IL-5 by a single T-cell clone are discussed.
Jabara
H H
HH
Division of Immunology, Children's Hospital, Boston, Massachusetts.
Ackerman
S J
SJ
Vercelli
D
D
Yokota
T
T
Arai
K
K
Abrams
J
J
Dvorak
A M
AM
Lavigne
M C
MC
Banchereau
J
J
De Vries
J
J
D002454:Cell Differentiation; D002999:Clone Cells; D004804:Eosinophils / Q000166:cytology* / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4; D015848:Interleukin-5; D007378:Interleukins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D007074:Immunoglobulin G; D015848:Interleukin-5; D007378:Interleukins; D011994:Recombinant Proteins; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma