Epidermis and lymphocyte interactions during a tuberculin skin reaction. I. Increased ETAF/IL-1 like activity, expression of tissue antigens and mixed skin lymphocyte reactivity.
Forty-one persons were tested for tuberculin skin reactivity. Epidermal cells (ECs) were isolated from the tuberculin reaction and from a contra lateral, non injected skin area. We found a significant increase of epidermal thymocyte activating factor (ETAF) in epidermis overlying a positive tuberculin reaction together with an increase of OKT6 and class II (HLA-DR) positive cells. Allogeneic lymphocytes proliferated significantly more when mixed with ECs from a positive tuberculin skin test. Injection of tuberculin per se or a negative reaction did not induce similar changes. The described model seems useful for functional studies of ECs and lymphocytes in patients with contact dermatitis.
Larsen
C G
CG
Department of Dermatology, Marselisborg Hospital, University of Aarhus, Denmark.
Ternowitz
T
T
Larsen
F G
FG
Thestrup-Pedersen
K
K
D000328:Adult; D000368:Aged; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D004247:DNA / Q000096:biosynthesis; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014374:Tuberculin Test
D000945:Antigens, Differentiation, T-Lymphocyte; D006684:HLA-DR Antigens; D007375:Interleukin-1; D004247:DNA