The effect of adenylate kinase activity on the rate and efficiency of energy transport from mitochondria to hexokinase.
The rate and efficiency of energy transport were examined in a system containing isolated rabbit heart mitochondria, hexokinase, adenylate kinase and low concentrations of adenine nucleotides. Oxygen consumption by mitochondria and glucose-6-phosphate synthesis by hexokinase were registered. It was found that when adenylate kinase is active both in mitochondria and in the environmental solution, the rate and efficiency (glucose-6-phosphate/O ratio) of glucose-6-phosphate formation considerably increase. The effects of adenylate kinase activity are fully abolished by diadenosine pentaphosphate, an inhibitor of adenylate kinase.
Dzeja
P
P
Kalvenas
A
A
Toleikis
A
A
Praskevicius
A
A
D000227:Adenine Nucleotides / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000263:Adenylate Kinase / Q000378:metabolism*; D000818:Animals; D003593:Cytoplasm / Q000201:enzymology; D015226:Dinucleoside Phosphates; D004735:Energy Transfer; D006593:Hexokinase / Q000378:metabolism*; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism*; D008954:Models, Biological; D009206:Myocardium / Q000201:enzymology; D010101:Oxygen Consumption; D010770:Phosphotransferases / Q000378:metabolism*; D011817:Rabbits
D000227:Adenine Nucleotides; D015226:Dinucleoside Phosphates; C012275:P(1),P(5)-di(adenosine-5'-)pentaphosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D006593:Hexokinase; D000263:Adenylate Kinase