title: Thio-analogues of inorganic pyrophosphate inhibit the replication of influenza virus A in vitro.
abstract: Mono- and bisthiopyrophosphate can inhibit the replication of influenza virus A/X49 in Madin-Darby canine kidney (MDCK) cells at concentrations at which no cytotoxic effect is observed after 3 days. The thiopyrophosphate analogues inhibit the RNA transcriptase activity of this virus possibly by chelating with an essential metal ion in the transcriptase complex. [31P]NMR spectroscopy indicates that bisthiopyrophosphate coordinates to zinc through sulphur and magnesium through oxygen which may influence the inhibitory properties of this compound with metal-containing enzymes.
authors.forename: D W
authors.initials: DW
authors.lastname: Hutchinson
authors.forename: M
authors.initials: M
authors.lastname: Naylor
authors.forename: P M
authors.initials: PM
authors.lastname: Cullis
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors; D011756:Diphosphates / Q000494:pharmacology*; D009980:Influenza A virus / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D011756:Diphosphates; C046011:bis(thiopyrophosphoric acid); C046010:monothiopyrophosphoric acid; D012321:DNA-Directed RNA Polymerases