[Electron microscopy of bovine leukosis viruses (BLV) in a fetal lamb spleen cell line].
The ultra-thin section method, the method of negative staining, and immunoelectron microscopy were used for detecting BLV and for determining its morphological characteristics in the FLS continual cell line used as a virus antigen producer for the ELISA test. Particles of C type, about 110 nm in size, having a structure corresponding to BLV, were detected in the FLS cells on the ultra-thin sections. The viruses were located extracellularly, in cytoplasmic vacuoles, and in different stages of maturation by budding from cell plasma membrane. BLV presence was also demonstrated by immunoelectron microscopy.
Valícek
L
L
Smíd
B
B
Machatková
M
M
D000818:Animals; D002460:Cell Line; D001909:Leukemia Virus, Bovine / Q000648:ultrastructure*; D012190:Retroviridae / Q000648:ultrastructure*; D012756:Sheep; D013154:Spleen / Q000382:microbiology*
Elektronová mikroskopie viru bovinní leukózy (BLV) v bunĕcné linii fetální jehnĕcí sleziny.