[Antitumor effect of human interferon-alpha A/D in mice (II). Activation of natural killer cells and suppression of metastasis].
Human recombinant interferon-alpha A/D (IFN-alpha A/D) is known to be effective on murine cells. We studied the in vivo effects of pure IFN-alpha A/D on murine splenic natural killer (NK) activity and experimentally induced pulmonary metastasis of B16-F10 melanoma. Intraperitoneal injection of IFN-alpha A/D augmented splenic NK activity, and suppressed melanoma metastasis. This suppression was abrogated by pretreatment of mice with anti-asialo GMl, but not with silica or carrageenan, showing that the effect of IFN-alpha A/D was dependent on NK cells. The suppression was strongest when IFN-alpha A/D treatment was given 12 hrs before or at the same time as tumor injection, showing that NK cells are most effective in the early phase of melanoma metastasis.
Yokoyama
T
T
Yoshie
O
O
Aso
H
H
Ebina
T
T
Ishida
N
N
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D007370:Interferon Type I / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D008545:Melanoma / Q000276:immunology* / Q000556:secondary; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D012822:Silicon Dioxide / Q000494:pharmacology; D013154:Spleen / Q000276:immunology
D007370:Interferon Type I; D012822:Silicon Dioxide; D002351:Carrageenan