Cloning of cDNAs coding for rat hepatic microsomal UDP-glucuronyltransferases.
Radioiodinated, affinity-purified, anti-UDP-glucuronyltransferase (UDPGT) antibodies have been used to isolate cDNAs coding for UDPGT(s) from a rat liver cDNA library cloned in the expression vector bacteriophage lambda gt11. The sizes of ten cloned cDNAs range from 0.3-2.1 kb. The identity of the cDNAs was confirmed by the hybrid-select translation and immunochemical analyses. Restriction mapping indicates that two classes of cDNA coding for different UDPGT mRNAs have been cloned.
Jackson
M R
MR
McCarthy
L R
LR
Corser
R B
RB
Barr
G C
GC
Burchell
B
B
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D014453:Glucuronosyltransferase / Q000235:genetics*; D008862:Microsomes, Liver / Q000201:enzymology*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains
D004274:DNA, Recombinant; D012333:RNA, Messenger; D004247:DNA; D014453:Glucuronosyltransferase; D004262:DNA Restriction Enzymes