Incorporation and turnover of phospholipid precursors in normal and tumoral glial cells in culture.
The incorporation and turnover of phospholipid precursors in cultured normal and tumoral glial cells was investigated during the plateau phase of growth. Glycerol was incorporated similarly by all cell types, and was renewed with a half-life of 19-37 hr. Acetate had a much longer half-life in primary cultures (50-75 hr) than in proliferative tumor cells (20-40 hr). Phosphate had a more rapid turnover rate in primary cultures (25 hr) than in proliferative tumor cells (50 hr). For all precursors, inositol- and choline phosphoglycerides had a faster turnover rate than other phospholipids.
Hugues
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P
Montaudon
D
D
Robert
J
J
D000085:Acetates / Q000378:metabolism; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D006224:Cricetinae; D005910:Glioma / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D006207:Half-Life; D008563:Membrane Lipids / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
D000085:Acetates; D008563:Membrane Lipids; D010710:Phosphates; D010743:Phospholipids; D003994:Bucladesine; D005990:Glycerol