Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3") (9).
The nucleotide sequence of the spc determinant of the Staphylococcus aureus transposon Tn554 has been determined. This gene encodes a spectinomycin adenyltransferase, AAD(9), that mediates resistance to spectinomycin but not to streptomycin. The sequence predicts a 260 amino acid protein of molecular weight 28,943. A spectinomycin-sensitive mutant (spc-1) contains a G----A transition resulting in substitution of threonine (ACA) for alanine (GCA) at residue 165. The predicted amino acid sequence is 36% homologous to that of a widely distributed, gram-negative streptomycin/spectinomycin adenyltransferase, AAD(3") (9), specified by the aadA determinant (Holingshead and Vapnek 1985).
Murphy
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D000595:Amino Acid Sequence; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D009154:Mutation; D009690:Nucleic Acid Conformation; D009713:Nucleotidyltransferases / Q000235:genetics*; D010957:Plasmids; D011523:Protoplasts / Q000201:enzymology; D000198:Spectinomycin / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic
D000198:Spectinomycin; D009713:Nucleotidyltransferases; C022682:streptomycin-spectinomycin adenylyltransferase; D004262:DNA Restriction Enzymes