Rapid subtyping of equine herpesvirus 1 with monoclonal antibodies.
Two antigenically similar subtypes of equine herpesvirus 1 (EHV-1) cause disease in horses. A procedure for rapid differentiation of the two EHV-1 subtypes with monoclonal antibodies was developed. Subtype-specific pools of monoclonal antibodies were constructed, characterized, and used in enzyme immunofiltration and indirect immunofluorescence assays to subtype 50 epizootiologically unrelated field isolates of EHV-1. Both assays allowed accurate subtype identification of each EHV-1 isolate with the monoclonal antibody pools. The subtyping procedures were simple and amenable to typing many isolates at one time and permitted unambiguous EHV-1 subtype identification with 3 h after isolation of the virus in tissue culture.
Yeargan
M R
MR
Allen
G P
GP
Bryans
J T
JT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002478:Cells, Cultured; D005374:Filtration; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000145:classification*; D004861:Herpesvirus 1, Equid / Q000145:classification* / Q000276:immunology; D006736:Horses / Q000382:microbiology; D006825:Hybridomas
D000911:Antibodies, Monoclonal