Ability of various alkylating agents to induce adaptive and SOS responses: a study with lacZ fusion.
We used alkA'-lacZ' and umuC'-lacZ' fused genes and determined the ability of various alkylating agents to induce adaptive and SOS responses. The degree of induction of expression of these genes was quantitatively measured by a simple colorimetric assay of beta-galactosidase activity. SN1 type methylating agents, such as N-methyl-N'-nitro-N-nitrosoguanidine and N-methyl-N-nitrosourea, were more effective inducers for the alkA than for the umuC system, while SN1 type ethylating agents, such as N-ethyl-N'-nitro-N-nitrosoguanidine and N-ethyl-N-nitrosourea, were more potent inducers for the umuC than for the alkA system. Similar but less striking effects on the two systems were obtained with SN2 type alkylating agents.
Otsuka
M
M
Nakabeppu
Y
Y
Sekiguchi
M
M
D000477:Alkylating Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D045647:DNA Glycosylases; D011088:DNA Ligases / Q000096:biosynthesis* / Q000235:genetics; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005038:Ethylnitrosourea / Q000494:pharmacology; D005798:Genes, Bacterial; D005813:Genes, Synthetic / Q000187:drug effects; D006147:Guanine / Q000378:metabolism; D007763:Lac Operon; D008745:Methylation; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008770:Methylnitrosourea / Q000494:pharmacology; D009699:N-Glycosyl Hydrolases / Q000235:genetics; D011118:Polynucleotide Ligases / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D000477:Alkylating Agents; D001426:Bacterial Proteins; D008769:Methylnitronitrosoguanidine; D006147:Guanine; D008770:Methylnitrosourea; D001616:beta-Galactosidase; C021565:3-methyladenine-DNA glycosylase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D011088:DNA Ligases; D011118:Polynucleotide Ligases; D005038:Ethylnitrosourea