Regulation of thymocyte proliferative response by macrophage-derived prostaglandin E2 and interleukin 1.
The antagonistic effects of macrophage (M phi)-derived interleukin 1 (IL 1) and prostaglandin E2 (PGE2) on thymocyte proliferation were uncoupled and studied. Elimination of PGE2 from the culture medium of prostaglandin E2-stimulated M phi was achieved by dialysis of the media or by indomethacin treatment of the M phi. IL1 secretion appears to be PGE2 independent. Experiments using exogenous PGE2 revealed a quantitative relationship between the two monokines. PGE2 (1.25 ng/ml) reduced the proliferative effect of IL 1 produced by 1.5 X 10(5) peritoneal M phi to 50%. This PGE2 dose increased significantly the levels of intracellular cAMP. Separation of peritoneal exudate M phi on a bovine serum albumin discontinuous gradient demonstrated that the main part of PGE2 synthesis was in a fraction of lower density, large M phi, whereas IL 1 activity was detected in all tested fractions.
Hayari
Y
Y
Kukulansky
T
T
Globerson
A
A
D000818:Animals; D002469:Cell Separation; D002474:Cell-Free System; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism; D003956:Dialysis; D015232:Dinoprostone; D004306:Dose-Response Relationship, Immunologic; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000145:classification / Q000378:metabolism*; D051379:Mice; D011458:Prostaglandins E / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*
D003470:Culture Media; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011458:Prostaglandins E; D000242:Cyclic AMP; D015232:Dinoprostone; D007213:Indomethacin