Quantitation of glucagon by radioreceptorassay.
Receptors for glucagon on rat liver membranes were characterized. They bound [125I] glucagon rapidly in a specific and saturable way. Addition of unlabelled glucagon displaced [125I] glucagon from the binding sites in a concentration dependent way. Concentrations from 10(-9) to 10(-8) M of glucagon caused a linear reduction of binding of labelled glucagon. This concentration interval was used for a three-point assay which fulfilled statistical requirements for validity. Individual assays normally resulted in potency estimates of high precision and statistical weight. Mean values for glucagon activity of preparations tested by receptor assay were within the fiducial limits (P = 0.95) for corresponding activity determined by the rabbit blood glucose method. The receptor assay is less time consuming and requires only part of one rat liver while the in vivo assay uses 16 rabbits. Thus, the receptor assay is less resource demanding and should serve well as a screening instrument for control of potency of glucagon preparations.
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D000818:Animals; D001667:Binding, Competitive; D001681:Biological Assay; D005934:Glucagon / Q000032:analysis* / Q000378:metabolism / Q000592:standards; D008099:Liver / Q000378:metabolism; D008297:Male; D011817:Rabbits; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism; D018027:Receptors, Glucagon; D012015:Reference Standards
D011956:Receptors, Cell Surface; D018027:Receptors, Glucagon; D005934:Glucagon