Immunohistochemical distribution of S-100 protein and glial fibrillary acidic protein in normal and neoplastic salivary glands.
Immunohistochemical localization of S-100 protein, its alpha and beta subunits, and glial fibrillary acidic protein (GFAP) in normal and neoplastic salivary glands was studied by the peroxidase-antiperoxidase method and immunoblot analysis. Positive immunostaining for S-100 protein was observed in pleomorphic adenoma, adenolymphoma, tubular adenoma, adenoid cystic carcinoma, acinic cell tumour, adenocarcinoma and carcinoma in pleomorphic adenoma. S-100 protein was localized in myoepithelial cells, epithelial cells of intercalated ducts and serous acinar cells of normal salivary gland. Both alpha and beta subunits of S-100 protein showed almost identical distribution in normal and neoplastic salivary glands, but skeletal muscle cells were alpha-positive/beta-negative whereas Schwann cells and fat cells were alpha-negative/beta-positive in the stroma and neighbouring tissue. GFAP was only found in pleomorphic adenoma and its malignant counterpart. Immunoblot analysis showed that the GFAP-related antigen consisted of several polypeptide bands with a molecular weight ranging between 35,000 to 50,000 daltons.
Nakazato
Y
Y
Ishida
Y
Y
Takahashi
K
K
Suzuki
K
K
D000230:Adenocarcinoma / Q000032:analysis; D008949:Adenoma, Pleomorphic / Q000032:analysis; D000941:Antigens / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis; D003528:Carcinoma, Adenoid Cystic / Q000032:analysis; D004848:Epithelium / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D010307:Parotid Neoplasms / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis*; D012468:Salivary Gland Neoplasms / Q000032:analysis* / Q000276:immunology; D012469:Salivary Glands / Q000032:analysis* / Q000166:cytology / Q000276:immunology
D000941:Antigens; D000951:Antigens, Neoplasm; D005904:Glial Fibrillary Acidic Protein; D010446:Peptide Fragments; D009418:S100 Proteins