title: High expression of an N-acetylglucosaminyltransferase III in 3'-methyl DAB-induced hepatoma and ascites hepatoma.
abstract: Ascites hepatoma AH-66 and 3'-Me-DAB-induced hepatoma of rats contain highly active N-acetylglucosaminyltransferase III (GnT-III) which catalyzes the addition of N-acetylglucosamine through a beta 1-4 linkage (bisecting N-acetylglucosamine) to the beta-linked mannose of the trimannosyl core of asparagine-linked sugar chains, whereas normal rat liver contains very little. The high activity was also detected in fetal rat liver, newborn rat liver, hyperplastic nodules and various transplantable hepatomas.
authors.affiliation: Department of Biochemistry, Osaka University Medical School, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nishikawa
authors.forename: S
authors.initials: S
authors.lastname: Fujii
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005260:Female; D005333:Fetus; D005964:Glucosyltransferases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000254:growth & development; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008115:Liver Regeneration; D008297:Male; D008749:Methyldimethylaminoazobenzene; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases; D009844:Oligosaccharides / Q000096:biosynthesis; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
chemical_list: D009844:Oligosaccharides; D008749:Methyldimethylaminoazobenzene; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C054599:beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase