Identification of a PDGF-like mitoattractant produced by NIH/3T3 cells after transformation with SV40.
It has previously been shown that fibroblastic cells transformed by SV40 exhibit a reduced requirement for PDGF for growth. In addition, NIH/3T3 cells lose both their chemotactic response to PDGF and specific cell surface binding of PDGF after transformation with SV40. We have now examined whether the SV40 transformed NIH/3T3 cells are producing a factor which acts similarly to PDGF. Our studies indicate that NIH/3T3 cells transformed with SV 40 produce a factor which shares many biological properties with PDGF. We were unable to detect this activity in conditioned media from nontransformed NIH/3T3 cells. The SV40/NIH/3T3 derived factor appears to possess both chemotactic and mitogenic activity for connective tissue cells but not endothelial or epithelial cells. Furthermore, in preliminary studies, this activity competes with 125I-PDGF for binding to smooth muscle cells. The biochemical properties of the SV40/NIH/3T3 derived factor are different from those of PDGF. The SV40 activity appears to reside in a heat labile acidic protein (pI less than 7.0) of MW less than 30,000 whereas PDGF is a heat stable basic protein (pI9.8) of 30,000 MW. Production of this factor may play a role in the decreased serum requirement for cell replication exhibited by SV40-transformed NIH/3T3 cells by supplying the cells with their own PDGF-like growth factor.
Bleiberg
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I
Harvey
A K
AK
Smale
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Grotendorst
G R
GR
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002472:Cell Transformation, Viral; D002630:Chemotactic Factors / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology; D002633:Chemotaxis; D004727:Endothelium / Q000166:cytology; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology; D006358:Hot Temperature; D051379:Mice; D008934:Mitogens / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000166:cytology; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D013539:Simian virus 40 / Q000502:physiology
D002630:Chemotactic Factors; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor