title: Physical characterization of the genome of the Myxococcus xanthus bacteriophage MX-8.
abstract: We have constructed a restriction map for the genome of bacteriophage MX-8 from Myxococcus xanthus using the enzymes PvuII, MboI, and EcoRI. The phage genome size, as determined by restriction analysis, is 51.7 +/- 0.6 Kb. Double digestions, redigestions of isolated fragments, and crossed-contact hybridization of partial digestion products show that the restriction map is circular. Restriction analysis and Southern hybridization show that the phage DNA molecules are packaged sequentially from a concatemer starting from a specific site which we have mapped. The DNA molecules have an average terminal redundancy of approximately 8% and are circularly permuted over at least 40% of the genome.
authors.forename: E
authors.initials: E
authors.lastname: Stellwag
authors.forename: J M
authors.initials: JM
authors.lastname: Fink
authors.forename: J
authors.initials: J
authors.lastname: Zissler
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000648:ultrastructure; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D008970:Molecular Weight; D009231:Myxococcales / Q000235:genetics*; D009690:Nucleic Acid Conformation
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D004262:DNA Restriction Enzymes