Thermoinducible transcription system for Bacillus subtilis that utilizes control elements from temperate phage phi 105.
We describe a thermoinducible-expression system for Bacillus subtilis which utilized an early promoter-operator sequence from temperate phage phi 105 and the thermolabile prophage repressor from the phage variant phi 105 cts23. The system operated at the transcriptional level to control expression in B. subtilis of the cat-86 gene derived from Bacillus pumilis. Details of the strategies used to isolate the early phage promoter are described. This promoter lay in close proximity to the prophage repressor gene on the phi 105 genome. The sequence of the early promoter differed from that of the vegetative B. subtilis consensus promoter by 1 base pair in both the -10 and -35 regions. We also present evidence that our phage-derived expression system could function in Escherichia coli to effect thermoinducible expression of the galK gene.
Osburne
M S
MS
Craig
R J
RJ
Rothstein
D M
DM
D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics; D005809:Genes, Regulator; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013045:Species Specificity; D013696:Temperature; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases