Identification of a T cell subset using a rabbit antibody raised against a T suppressor molecule.
Rabbit antiserum to a unique component of an Ag-binding Ts-factor was generated by repeated immunization with purified 30-kDa protein isolated from Fd11 Ts factor (11). This antiserum (anti-p30) was shown to recognize cell surface determinants expressed on the Ts hybridomas Fd11 and A10 but not the fusion partner BW5147. Furthermore, this antiserum was shown to bind to approximately 4% of thymocytes and 10% of nylon wool-purified splenic T cells from all strains of mice tested. Sorting nylon wool-purified T cells from DBA/2 mice for the CD4+ and CD8+ subsets revealed both populations contained cells that bound anti-p30. In addition, when CD4-8- thymocytes were examined for anti-p30 labeling, it was found that about 30% of this enriched population also expressed p30 molecules. In a functional study, anti-p30 was able to neutralize the suppressive effects of Fd11 on a specific assay for in vitro antibody synthesis against ferredoxin.
North
J
J
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Weaver
M S
MS
Stammers
A T
AT
Chu
N R
NR
Chan
A
A
Levy
J G
JG
D000818:Animals; D001666:Binding Sites, Antibody; D002460:Cell Line; D002474:Cell-Free System; D005434:Flow Cytometry; D006825:Hybridomas / Q000032:analysis; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011817:Rabbits; D013045:Species Specificity; D013491:Suppressor Factors, Immunologic / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D013601:T-Lymphocytes / Q000032:analysis / Q000145:classification* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000145:classification* / Q000276:immunology
D007106:Immune Sera; D013491:Suppressor Factors, Immunologic