Preparation of bacteriophage lambda DNA using the TL-100 ultracentrifuge.
A procedure for the preparation of DNA from bacteriophage lambda is described, using the Beckman TL-100 bench-top ultracentrifuge. The procedure involves growth of phage in agar plates, precipitation with polyethylene glycol, and a single centrifugation in cesium chloride under conditions that disrupt the phage coat. The method avoids the use of enzymes, ion exchange resins, and phenol. It can be completed in less than a day. The resulting DNA is of good purity and is easily cuttable by restriction enzymes.
Buckley
M F
MF
Department of Pathology and Immunology, Monash University Medical School, Alfred Hospital, Prahran, Victoria, Australia.
Goding
J W
JW
D010582:Bacteriophage lambda / Q000032:analysis*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D002586:Cesium; D011232:Chemical Precipitation; D002712:Chlorides; D004279:DNA, Viral / Q000302:isolation & purification*; D000431:Ethanol; D011092:Polyethylene Glycols
D002712:Chlorides; D004279:DNA, Viral; D002586:Cesium; D000431:Ethanol; D011092:Polyethylene Glycols; C028019:cesium chloride