title: Stage-specific patterns of collagen gene expression during development of Caenorhabditis elegans.
abstract: Collagens are the major protein components of the Caenorhabditis elegans cuticle and are encoded by a large family of 40 to 150 closely related but nonidentical genes. We have determined temporal patterns of mRNA accumulation for a large number of collagen genes by screening recombinant phages and plasmids containing cloned collagen genes under high stringency conditions with 32P-labeled cDNA preparations specific for eggs or three postembryonic molts. We find that collagen mRNA levels are regulated both temporally and quantitatively during C. elegans development. Most genes studied exhibit one of four patterns of mRNA accumulation which correlate with changes in cuticle morphology and collagen protein composition during development. Our results suggest that, in general, there is a progressive activation of new collagen genes during normal development.
authors.forename: G N
authors.initials: GN
authors.lastname: Cox
authors.forename: D
authors.initials: D
authors.lastname: Hirsh
mesh_terms: D000818:Animals; D002107:Caenorhabditis / Q000254:growth & development*; D003094:Collagen / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D003094:Collagen; D004262:DNA Restriction Enzymes