Demonstration of T antigens on the surface of cells transformed with primate polyoma viruses.
Primate polyoma virus-transformed hamster, mouse, and rat cell lines were examined by indirect immunofluorescence staining for cell surface-associated T antigens, by using a rabbit antiserum prepared against sodium dodecyl sulfate-denatured large T antigen of simian virus 40 (anti-SV40-SDS-T serum). Positive surface staining was shown not only on SV40-transformed cells, but also on BK and JC virus-transformed cells. In contrast, normal cells and cells transformed with mouse polyoma-, human adeno-, and murine sarcoma viruses were negative. The data on SV40-transformed cells confirmed the reports of others demonstrating the cell surface location of SV40 large T antigen, and the data on BK and JC virus-transformed cells proved that these cells have cell-surface T antigens that cross-react with anti-SV40-SDS-T serum.
Ohno
T
T
Yamaguchi
N
N
Uchida
S
S
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000954:Antigens, Surface / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D051379:Mice; D011120:Polyomavirus / Q000276:immunology*; D051381:Rats; D014764:Viral Proteins / Q000032:analysis*
D000952:Antigens, Polyomavirus Transforming; D000954:Antigens, Surface; D000956:Antigens, Viral; D000957:Antigens, Viral, Tumor; D014764:Viral Proteins