Interaction of adriamycin with human erythrocyte membranes. Role of the negatively charged phospholipids.
The interaction of the antitumor compound adriamycin with human erythrocyte membranes, used as models of target cell membranes, has been studied using circular dichroism measurements. In order to elucidate the nature of the sites involved in the electrostatic interaction between adriamycin and erythrocyte membranes, its interaction with the following macromolecular systems was studied: phosphatidylserine-containing small unilamellar vesicles (SUV), prepared from total lipid extracts of erythrocytes, sialic acid-depleted erythrocyte ghosts and mucopolysaccharides. We have shown that the interaction between adriamycin and carboxylate groups is very weak and that negatively charged phosphate groups, in the case of membranes, or sulfate groups, in the case of mucopolysaccharides, are responsible for the prime interaction of adriamycin with these macromolecular systems.
Garnier-Suillerot
A
A
Laboratoire de Chimie Bioinorganique (UA CNRS 198), UFR de Santé, Médecine et Biologie Humaine, Université Paris-Nord, Bobigny, France.
Gattegno
L
L
D002942:Circular Dichroism; D004317:Doxorubicin / Q000097:blood*; D004563:Electrochemistry; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000097:blood; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D046911:Macromolecular Substances; D008563:Membrane Lipids / Q000097:blood; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000097:blood; D010718:Phosphatidylserines / Q000097:blood; D010743:Phospholipids / Q000097:blood*; D011402:Pronase / Q000494:pharmacology; D012794:Sialic Acids / Q000097:blood; D013049:Spectrin / Q000502:physiology; D014357:Trypsin / Q000494:pharmacology
D006025:Glycosaminoglycans; D008081:Liposomes; D046911:Macromolecular Substances; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D010743:Phospholipids; D012794:Sialic Acids; D013049:Spectrin; D004317:Doxorubicin; D009439:Neuraminidase; D014357:Trypsin; D011402:Pronase; D019158:N-Acetylneuraminic Acid