Evidence for an association between calmodulin and membrane patches containing gonadotropin-releasing hormone--receptor complexes in cultured gonadotropes.
participation of calmodulin, clathrin, and actin in receptor mediated endocytosis of gonadotropin-releasing hormone (GnRH) was studied in an in vitro system of dispersed pituitary cells with a triple staining procedure. Cells were incubated in D-Lys6-Pro9-Des10-GnRH-biotin and stained with avidin-peroxidase-diaminobenzidine. Calmodulin, clathrin, and actin as well as luteinizing hormone were identified by indirect immunofluorescence with FITC- and rhodamine-labeled second antibody. The results indicate a close spatial association of calmodulin, but not of clathrin and actin, with GnRH-containing plasma membrane patches.
Jennes
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L
Bronson
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Stumpf
W E
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Conn
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PM
D000199:Actins / Q000378:metabolism; D000818:Animals; D002147:Calmodulin / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D002966:Clathrin / Q000378:metabolism; D003034:Coated Pits, Cell-Membrane / Q000648:ultrastructure; D004705:Endocytosis; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007158:Immunologic Techniques; D007986:Luteinizing Hormone / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011966:Receptors, LHRH
D000199:Actins; D002147:Calmodulin; D002966:Clathrin; D011956:Receptors, Cell Surface; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone