Plasma membrane and cytoskeletal constituents in Leishmania mexicana.
The presence and the localization of actin, spectrin and ankyrin are studied by immunofluorescence and immunoblotting in Leishmania mexicana promastigotes growing in vitro. These proteins, amphitropic in nature, coexist both in soluble and insoluble forms. Our results demonstrate that the Triton insoluble form of these proteins constitutes beside tubulin the cytoskeletal scaffold of promastigotes in close association with the plasma membrane, the axoneme and the basal body of the parasite.
Dagger
F
F
Department of Cell Biology, Faculty of Sciences, Universidad Central de Venezuela, Caracas.
Dunia
I
I
Hernandez
A G
AG
Pradel
L A
LA
Benedetti
E L
EL
D000199:Actins / Q000032:analysis; D000818:Animals; D017487:Ankyrins; D001798:Blood Proteins / Q000032:analysis; D015153:Blotting, Western; D002462:Cell Membrane / Q000032:analysis; D003598:Cytoskeletal Proteins / Q000032:analysis*; D003599:Cytoskeleton / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007894:Leishmania mexicana / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis; D013049:Spectrin / Q000032:analysis; D013329:Structure-Activity Relationship
D000199:Actins; D017487:Ankyrins; D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D013049:Spectrin