title: A comparative survey of several bacterial aa3-type cytochrome c oxidases.
abstract: The aa3-type cytochrome c oxidases purified from Nitrobacter agilis, Thiobacillus novellus, Nitrosomonas europaea, and Pseudomonas AM 1 were compared. They have haem a and copper atom as the prosthertic groups and show alpha and gamma absorption peaks at around 600 and 440 nm, respectively. Each oxidase molecule is composed of two kinds of subunits. The N. agilis oxidase has 2 moles of haem a and 2 atoms of copper in the minimal structural unit composed of one molecule each of the two kinds of subunits, while the T. novellus enzyme seems to contain one molecule of the haem and one atom of the metal in the unit. The N. europaea oxidase shows very low affinity for carbon monoxide. Each oxidase reacts rapidly with some eukaryotic cytochromes c as well as with its native cytochrome c. The cytochrome c oxidase activity of the N. agilis oxidase is 50% inhibited by 1 microM KCN, while 50% inhibition of the activity requires 100 microM KCN in the case of the N. europaea enzyme.
authors.forename: T
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumori
authors.forename: T
authors.initials: T
authors.lastname: Yamazaki
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Nakayama
mesh_terms: D001386:Azides / Q000494:pharmacology; D002248:Carbon Monoxide / Q000378:metabolism; D002308:Cardiolipins / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D003574:Cytochrome c Group / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008970:Molecular Weight; D009576:Nitrobacter / Q000201:enzymology*; D009606:Nitrosomonas / Q000201:enzymology*; D011190:Potassium Cyanide / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology*; D019810:Sodium Azide; D013054:Spectrophotometry, Atomic; D013855:Thiobacillus / Q000201:enzymology*
chemical_list: D001386:Azides; D002308:Cardiolipins; D003574:Cytochrome c Group; D002248:Carbon Monoxide; D019810:Sodium Azide; D003576:Electron Transport Complex IV; D011190:Potassium Cyanide