Antibodies against a synthetic peptide as a probe for the kinase activity of the avian EGF receptor and v-erbB protein.
The transforming protein v-erbB of avian erythroblastosis virus (AEV) displays extensive sequence homology with the presumptive protein-tyrosine kinase domain of the human EGF receptor and with the src protein-tyrosine kinase family of oncogenes. However, no kinase activity has previously been demonstrated for the v-erbB protein. Here antibodies generated against a synthetic peptide from the C terminus of human EGF receptor are shown to immunoprecipitate the EGF receptor from human and avian cells, as well as the v-erbB proteins from AEV-transformed cells that become phosphorylated on tyrosine residues upon the addition of gamma-32P-ATP. The immunoprecipitates are also able to phosphorylate exogenous tyrosine-containing substrates. Hence, it is likely that both avian EGF receptor and v-erbB proteins are protein tyrosine-specific protein kinases. Since the kinase activity of v-erbB protein cannot be regulated by EGF, it is proposed that the tyrosine protein kinase function of v-erbB may be constitutively activated.
Kris
R M
RM
Lax
I
I
Gullick
W
W
Waterfield
M D
MD
Ullrich
A
A
Fridkin
M
M
Schlessinger
J
J
D001355:Alpharetrovirus / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D001354:Avian Leukosis Virus / Q000201:enzymology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D066246:ErbB Receptors; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D009857:Oncogenes; D010446:Peptide Fragments / Q000276:immunology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000276:immunology / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D000941:Antigens; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D014764:Viral Proteins; D011494:Protein Kinases; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases