title: Modulation of collagen production in cultured fibroblasts by a low-frequency, pulsed magnetic field.
abstract: Primary cultures of chicken tendon fibroblasts have been exposed for various periods to a low-frequency, pulsed magnetic field, and the effects on protein and collagen synthesis have been examined by radioisotopic incorporation. Total protein synthesis was increased in confluent cells treated with a pulsed magnetic field for the last 24 h of culture as well as in cells treated for a total of 6 days. However, in 6 day-treated cultures, collagen accumulation was specifically enhanced as compared to total protein, whereas after short-term exposure, collagen production was increased only to the same extent as total protein. Levels of cyclic AMP were significantly decreased after 6-day pulsed magnetic field treatment, probably as a consequence of diminished adenylate cyclase activity. Exposure to pulsed magnetic field had no effect on cell proliferation or collagen phenotype. These results indicate that a pulsed magnetic field can specifically increase production of collagen, the major differentiated function of fibroblasts, possibly by altering cyclic-AMP metabolism.
authors.forename: J C
authors.initials: JC
authors.lastname: Murray
authors.forename: R W
authors.initials: RW
authors.lastname: Farndale
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000378:metabolism; D004574:Electromagnetic Fields; D055590:Electromagnetic Phenomena; D005347:Fibroblasts / Q000378:metabolism*; D006909:Hydroxyproline / Q000378:metabolism; D011392:Proline / Q000378:metabolism
chemical_list: D003094:Collagen; D011392:Proline; D000242:Cyclic AMP; D006909:Hydroxyproline