Suppression of contractile force in muscle fibers by antibody to myosin subfragment 2.
Polyclonal antibody directed against the subfragment-2 region of myosin was purified by affinity chromatography. Skinned muscle fibers that had been preincubated with antibody were able to sustain only 7% of the active isometric force generated by control fibers. The effect of antibody on force production could not be accounted for by inhibition of ATP turnover.
Lovell
S
S
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
Karr
T
T
Harrington
W F
WF
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D002645:Chickens; D005998:Glycine; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D009119:Muscle Contraction; D015879:Myosin Subfragments; D009218:Myosins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D051381:Rats
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D015879:Myosin Subfragments; D010446:Peptide Fragments; D000251:Adenosine Triphosphatases; D009218:Myosins; D005998:Glycine