Activation of protein kinase C potentiates isoprenaline-induced cyclic AMP accumulation in rat pinealocytes.
The pineal gland has proven to be an excellent model for the study of adrenergic control systems. Noradrenaline, released from sympathetic nerve terminals in the pineal gland, regulates a large nocturnal increase in melatonin synthesis by stimulating the activity of arylalkylamine N-acetyltransferase (NAT, EC 2.3.1.87) 30-70-fold. An essential step in both the induction and maintenance of high NAT activity is an increase in intracellular cyclic AMP. Noradrenaline acts via beta-adrenoceptors to increase pineal cyclic AMP by activating adenylate cyclase, and the activation of pineal alpha 1-adrenoceptors potentiates beta-adrenergic stimulation not only of NAT but of both cyclic AMP and cyclic GMP. Here we describe investigations designed to test whether alpha 1-adrenergic potentiation of beta-adrenergic stimulation of pineal cyclic AMP involves protein kinase C. Our results suggest that kinase activation is involved and the data provide the first demonstration of a synergistic interaction between Ca2+-phospholipid-dependent protein kinase (protein kinase C) and neurotransmitter-dependent stimulation of cyclic AMP.
Sugden
D
D
Vanecek
J
J
Klein
D C
DC
Thomas
T P
TP
Anderson
W B
WB
D000818:Animals; D002118:Calcium / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism*; D004075:Diglycerides / Q000494:pharmacology; D004357:Drug Synergism; D005260:Female; D007545:Isoproterenol / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology; D010870:Pineal Gland / Q000378:metabolism*; D011493:Protein Kinase C; D011494:Protein Kinases / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D004075:Diglycerides; D010703:Phorbol Esters; D010656:Phenylephrine; D000242:Cyclic AMP; D011494:Protein Kinases; D011493:Protein Kinase C; D007545:Isoproterenol; D002118:Calcium