Macrophage colony-stimulating factor (M-CSF) enhances the capacity of murine macrophages to secrete oxygen reduction products.
The capacity of macrophage colony-stimulating factor (M-CSF) to enhance respiratory burst activity in peritoneal macrophages was measured. Macrophages incubated for 48 hr or more with concentrated L cell-conditioned medium as a source of M-CSF released two to three times as much O2- in response to PMA as did unexposed macrophages. Stimulation was noted at concentrations of colony-stimulating activity from 0.1 to 2000 U/ml and was maximal at 10 to 100 U/ml. Purified, endotoxin-free CSF enhanced secretion to a similar degree as unpurified L cell-conditioned medium. Release of O2- by M-CSF macrophages occurred over 60 min and was triggered by opsonized zymosan as well as PMA. H2O2 release was also enhanced in macrophages exposed to both unpurified and purified M-CSF. These data indicate that M-CSF enhances the capacity of mature macrophages to release oxygen reduction products, and they are consistent with reports that CSF can stimulate the release of other secretory products.
Wing
E J
EJ
Ampel
N M
NM
Waheed
A
A
Shadduck
R K
RK
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D005260:Female; D006861:Hydrogen Peroxide / Q000096:biosynthesis*; D007700:Kinetics; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D013481:Superoxides / Q000096:biosynthesis*; D015054:Zymosan / Q000494:pharmacology
D001500:BCG Vaccine; D003115:Colony-Stimulating Factors; D013481:Superoxides; D015054:Zymosan; D006861:Hydrogen Peroxide