Determination of the catalytic activity of cyclomaltodextrin glucanotransferase by maltotriose-methylorange assay.
A kinetic assay of cyclomaltodextrin glucanotransferase (EC 2.4.1.19), based on the use of methylorange dye as the indicator of cyclodextrins, was studied and the reaction verified with independent HPLC analyses. The assay was optimized for the enzyme of an alkalophilic Bacillus sp. (ATCC 21783). Described enzymological data provide a reasonable background for detection of possible errors. Numerical correlations with previous assays are presented. This method can be adopted as a standard analysis of any cyclomaltodextrin-forming enzyme.
Mäkelä
M J
MJ
Department of Biochemistry, University of Turku, Finland.
Korpela
T K
TK
D001391:Azo Compounds; D001407:Bacillus / Q000201:enzymology; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D013053:Spectrophotometry; D013379:Substrate Specificity; D014312:Trisaccharides
D001391:Azo Compounds; D003505:Cyclodextrins; D007202:Indicators and Reagents; D014312:Trisaccharides; C008317:maltotriose; C100258:methyl orange; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase