title: Regulation of pro-opiomelanocortin synthesis by dopamine and cAMP in the amphibian pituitary intermediate lobe.
abstract: The modulation of pro-opiomelanocortin (POMC) synthesis in Xenopus laevis pituitary intermediate lobe (IL) during background adaptation and the role of dopamine and cAMP in mediating this effect were examined. Neurointermediate lobes (NILs) were pulselabeled in vitro with [3H]arginine and analyzed for POMC synthesis by acid-urea gel electrophoresis. After black background adaptation of the animal (7 days), POMC synthesis increased 5-6-fold, while after white background adaptation (7 days), POMC synthesis decreased by 76%. Dopamine (50 microM) suppressed POMC synthesis in NILs in culture. In the absence of dopamine, POMC synthesis was stimulated. Several experiments were conducted to determine the category of dopamine receptor in the X. laevis IL. A D-2 dopamine receptor agonist inhibited immunoreactive alpha-MSH release from the NIL in a D-2 antagonist-reversible manner. A D-1 receptor agonist or antagonist did not alter the release of immunoreactive alpha-MSH from the NIL. Dopamine (10 microM) inhibited forskolin-stimulated cAMP accumulation. In addition, dopamine inhibition of POMC synthesis in cultured ILs was reversed by 8-Br-cAMP. These studies suggest that white background adaptation results in stimulation of the X. laevis D-2 receptor, which reduces cAMP production and POMC synthesis. Conversely, during black background adaptation the IL D-2 receptor is not stimulated, leading to increased cAMP production and POMC synthesis.
authors.forename: Y P
authors.initials: YP
authors.lastname: Loh
authors.forename: B
authors.initials: B
authors.lastname: Myers
authors.forename: B
authors.initials: B
authors.lastname: Wong
authors.forename: D C
authors.initials: DC
authors.lastname: Parish
authors.forename: M
authors.initials: M
authors.lastname: Lang
authors.forename: M E
authors.initials: ME
authors.lastname: Goldman
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000222:Adaptation, Physiological; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D005576:Colforsin; D003116:Color; D000242:Cyclic AMP / Q000378:metabolism / Q000502:physiology*; D004224:Diterpenes / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology*; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis*; D011954:Receptors, Dopamine / Q000032:analysis; D014982:Xenopus laevis
chemical_list: D004224:Diterpenes; D011954:Receptors, Dopamine; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011333:Pro-Opiomelanocortin; D000242:Cyclic AMP; D004298:Dopamine