Identification of transformation-specific proteins synthesized in cryptovirogenic mammalian cells.
The cryptovirogenic PR-RSH-19 (H-19) hamster cell line, derived originally from a tumour induced by the virus rescued from XC cells by transfection, was studied. The pp60v-src protein was detected by immunoprecipitation with sera from tumour-bearing rabbits (TBR sera) from lysates of H-19 cells. A closely related protein p60 was precipitated by antiserum obtained from hamsters with tumours induced by H-19 cells. The pp60v-src of H-19 cells possesses an associated kinase activity. These results are consistent with the notion that the detected pp60v-src protein is a product of the cryptic sequences in H-19 cells.
Grófová
M
M
Bízik
J
J
Blahová
S
S
Geryk
J
J
Svoboda
J
J
D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology; D001358:Avian Sarcoma Viruses; D002460:Cell Line; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D006224:Cricetinae; D008647:Mesocricetus; D009363:Neoplasm Proteins / Q000276:immunology / Q000302:isolation & purification*; D015688:Oncogene Protein pp60(v-src); D011494:Protein Kinases / Q000302:isolation & purification; D011817:Rabbits; D012513:Sarcoma, Experimental / Q000032:analysis* / Q000382:microbiology; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*
D000912:Antibodies, Neoplasm; D009363:Neoplasm Proteins; D014764:Viral Proteins; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)