The large scale purification of ubiquitin from human erythrocytes.
A simple, reproducible method for the large-scale purification of active ubiquitin from human erythrocytes is described. Erythrocytes contain 100 micrograms free ubiquitin per cc of packed cells, of which 44% can be recovered in homogeneous form by a combination of heat treatment, ammonium sulfate fractionation, and ion exchange chromatography.
Haas
A L
AL
Wilkinson
K D
KD
D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D004912:Erythrocytes / Q000032:analysis*; D006609:High Mobility Group Proteins / Q000097:blood*; D006358:Hot Temperature; D006801:Humans; D014452:Ubiquitins / Q000097:blood*
D006609:High Mobility Group Proteins; D014452:Ubiquitins