Lymphocyte activation by purified HLA-DR molecules fused into autochthonous "stimulating cells".
Affinity-purified Ia molecules derived from the Daudi cell line were reconstituted into vesicles with Sendai virus envelope glycoproteins. These vesicles inserted into human peripheral leukocytes could induce stimulation of autologous lymphocytes, as measured by thymidine uptake, 6 days later. It is suggested that this method could provide a means to study allostimulation at the molecular level.
Diu
A
A
Abikar
K
K
Rode
H N
HN
Gordon
J
J
D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000276:immunology; D010222:Parainfluenza Virus 1, Human; D014759:Viral Envelope Proteins
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D008565:Membrane Proteins; D014759:Viral Envelope Proteins