title: Epstein-Barr virus induces a unique pyrimidine deoxynucleoside kinase activity in superinfected and virus-producer B cell lines.
abstract: Epstein-Barr (EB) virus induces a new pyrimidine deoxynucleoside kinase [thymidine kinase (dTk)] activity in Raji B lymphocyte cells after superinfection. This dTk activity is also present in small amounts in the HR-1 virus-producer cell line and in larger amounts in the B95-8 virus-producer line. The dTk activity induced by EB virus coelutes from DEAE-cellulose columns with deoxycytidine kinase (dCk) activity and elutes as a broad peak well separated from the large peaks of cellular dTk and dCk activities. This EB virus-induced pyrimidine deoxynucleoside kinase activity from HR-1 cells differs from cellular kinases in most basic biochemical properties but shares certain properties with the herpes simplex virus dTk.
authors.forename: T
authors.initials: T
authors.lastname: Stinchcombe
authors.forename: W
authors.initials: W
authors.lastname: Clough
mesh_terms: D001402:B-Lymphocytes / Q000201:enzymology*; D002051:Burkitt Lymphoma / Q000201:enzymology; D002460:Cell Line; D002472:Cell Transformation, Viral; D003842:Deoxycytidine Kinase / Q000096:biosynthesis* / Q000302:isolation & purification*; D004790:Enzyme Induction; D004854:Herpesvirus 4, Human / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D010770:Phosphotransferases / Q000096:biosynthesis* / Q000302:isolation & purification*; D013937:Thymidine Kinase / Q000096:biosynthesis* / Q000302:isolation & purification; D014316:Tritium
chemical_list: D014316:Tritium; D010770:Phosphotransferases; D013937:Thymidine Kinase; D003842:Deoxycytidine Kinase