Rapid separation of Pneumocystis carinii from lung lavage fluids.
A simple, rapid procedure to separate Pneumocystis carinii obtained by lavage of lungs of steroid-treated rats from rat leukocytes is described. Commercially available monoclonal antibody to rat common leukocyte determinants is used to sediment the leukocytes, resulting in supernatant fluid containing P. carinii cells virtually free of intact rat cells.
Pesanti
E L
EL
University of Connecticut Health Center, Newington, Connecticut 06111.
McCarron
B A
BA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000469:parasitology*; D002498:Centrifugation; D006649:Histocompatibility Antigens / Q000276:immunology; D017493:Leukocyte Common Antigens; D007962:Leukocytes / Q000276:immunology; D008168:Lung / Q000469:parasitology*; D011010:Pneumocystis / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis* / Q000469:parasitology; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens; D017493:Leukocyte Common Antigens