Structure of the fructose-containing K52 capsular polysaccharide of uropathogenic Escherichia coli O4:K52:H-.
The chemical structure of the K52 antigenic capsular polysaccharide (K52 antigen) of Escherichia coli O4:K52:H- was elucidated by composition, nuclear magnetic resonance spectroscopy, methylation, periodate oxidation before and after graded acid hydrolysis and by oligosaccharide analysis. The polysaccharide consists of a backbone of alpha-galactose units interlinked between C1 and C3 by phosphodiester bridges. This poly(alpha-galactosyl-phosphate) is substituted at C2 of each galactose unit by beta-fructofuranose residues. About 80% of the galactose units are O-acetylated at C4 and about 10% of the fructose units are both O-acetylated and O-propionylated at C1. The K52 polysaccharide has an average molecular mass of 34 kDa, thus consisting of approximately 65 fructosyl-galactosyl-phosphate repeating units.
Hofmann
P
P
Jann
B
B
Jann
K
K
D000942:Antigens, Bacterial / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli / Q000276:immunology*; D005632:Fructose / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; D005632:Fructose; C009288:metaperiodate