title: Antibodies to human T lymphotropic virus type III demonstrated by a dot immunobinding assay.
abstract: A dot immunobinding (DIB) technique was applied to the demonstration of antibodies to human T-lymphotropic virus type III (HTLV-III). By this technique IgG antibodies to HTLV-III were demonstrated in six of six Swedish patients with acquired immune deficiency syndrome and in 37 of 39 (95%) Swedish homosexual men with persistent generalized lymphadenopathy but in none of 50 Swedish healthy blood donors. Findings obtained by this method showed complete concordance with those obtained by an enzyme-linked immunosorbent assay. All sera positive by DIB were also positive by Western blotting. The DIB assay is simple and well suited for screening of sera for HTLV-III antibodies.
authors.forename: G
authors.initials: G
authors.lastname: Biberfeld
authors.forename: U
authors.initials: U
authors.lastname: Bredberg-Råden
authors.forename: B
authors.initials: B
authors.lastname: Böttiger
authors.forename: P
authors.initials: P
authors.lastname: Biberfeld
authors.forename: L
authors.initials: L
authors.lastname: Morfeldt-Månsson
authors.forename: J
authors.initials: J
authors.lastname: Suni
authors.forename: A
authors.initials: A
authors.lastname: Vaheri
authors.forename: C
authors.initials: C
authors.lastname: Saxinger
authors.forename: R
authors.initials: R
authors.lastname: Gallo
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000382:microbiology; D000914:Antibodies, Viral / Q000032:analysis*; D001782:Blood Donors; D017977:Deltaretrovirus / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D008206:Lymphatic Diseases / Q000276:immunology* / Q000382:microbiology; D008297:Male
chemical_list: D000914:Antibodies, Viral