A modified photometric quantification of skin surface lipids sampled using the cigarette paper method.
In severe cases of acne conglobata the use of substances which considerably reduce the sebum excretion rate, such as 13-cis-retinoic acid, is reasonable. To monitor the effect of such preparation a noninvasive and accurate method for measuring skin surface lipids (SSLs) is required. A new modified photometric quantification of SSLs sampled using the cigarette paper method based on reaction with phosphovanilic reagent was introduced. Our overall results indicate that a 3-h SSLs assessment measured photometrically is as accurate as the gravimetric technique; it is reproducible in situations with low and high levels of SSLs.
Bolanca-Bumber
S
S
Department of Dermatology, Clinical Center Zagreb, Yugoslavia.
Basta-Juzbasić
A
A
Kansky
A
A
Gross
T
T
D000152:Acne Vulgaris / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000032:analysis*; D010209:Paper; D012629:Sebum / Q000032:analysis; D012867:Skin / Q000032:analysis*; D013053:Spectrophotometry
D008055:Lipids