Molecular weight of human brain neutral sphingomyelinase determined in situ by the radiation inactivation method.
The radiation inactivation method was used to determine the molecular weight of membrane-bound neutral sphingomyelinase from normal human brain. Inactivation curves showed a molecular mass of 167,000 +/- 32,000. Molecular weights of two control enzymes, beta-N-acetylglucosaminidase and nonspecific beta-glucosidase, determined by the same procedure, were consistent with previous reports.
Levade
T
T
Potier
M
M
Salvayre
R
R
Douste-Blazy
L
L
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008970:Molecular Weight; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism* / Q000528:radiation effects; D001617:beta-Glucosidase / Q000378:metabolism
D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase; D001617:beta-Glucosidase; D000118:Acetylglucosaminidase