Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor.
DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino acid residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.
Furutani
Y
Y
Notake
M
M
Yamayoshi
M
M
Yamagishi
J
J
Nomura
H
H
Ohue
M
M
Furuta
R
R
Fukui
T
T
Yamada
M
M
Nakamura
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008264:Macrophages / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D013045:Species Specificity
D007375:Interleukin-1; D011498:Protein Precursors; D012333:RNA, Messenger; C043830:interleukin 1 precursor; D004247:DNA; D004262:DNA Restriction Enzymes