Quantitative and functional change in T cells of primiparous mice following injection of benzo(a)pyrene at the second trimester of pregnancy.
Progeny from benzo(a)pyrene (BP) exposed (150 micrograms/g body weight) primiparous mothers, injected during the second trimester of pregnancy, are severely compromised immunologically. In view of maternal-fetal associations, we studied, during pregnancy and postpartum, the quantitative and functional status of the maternal T cell population in the thymus and/or spleen. In the thymus, there is an exacerbated depression in the amounts of thymocytes, (theta +, Lyt 1+, Lyt 2+ cells) during pregnancy relative to the corn oil-injected controls, which is sustained postpartum. In the spleen, while there are inconsistencies in the level of theta + cells, The Lyt 1+ are depressed postpartum relative to virgins, but the Lyt 2+ are enhanced during pregnancy and postpartum, reaching levels greater than 700-fold of controls. In controls, while the number of Lyt 1+ cells was higher than BP-exposed mice or virgins during pregnancy, they virtually disappeared postpartum. A similar but reversed image is mirrored by the Lyt 2+ cells, i.e., they were virtually absent during pregnancy but increased postpartum. Splenic allogeneic and syngeneic mixed lymphocyte responses were subnormal. The data show that BP disrupts the maternal T cell repertoire, leading to an accumulation of Lyt 1-2+ cells, and suggest that splenic disruption may be due to changes in the differentiation potential of T precursor cells. These changes not only are most likely to affect maternal defenses, but also may have a direct bearing on the establishment of progeny immune status.
Urso
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Department of Microbiology and Immunology, Morehouse School of Medicine, Atlanta, GA 30310-1495.
Johnson
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RA
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000187:drug effects; D007958:Leukocyte Count; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008809:Mice, Inbred C3H; D009929:Organ Size / Q000187:drug effects; D010298:Parity; D011247:Pregnancy; D013601:T-Lymphocytes / Q000145:classification / Q000187:drug effects* / Q000276:immunology
D001564:Benzo(a)pyrene