The simian virus 40 sequences between 0.169 and 0.423 map units are not essential to immortalize early-passage rat embryo cells.
F8dl is a simian virus 40 early-region deletion mutant that lacks the sequences between 0.169 and 0.423 map units. We show that cloned F8dl DNA immortalized early-passage Fisher rat embryo cells with an efficiency that was about 20% of that of cloned wild-type simian virus 40 DNA. In contrast, we detected no immortalized colonies when we transfected the cells with DNA of five other early-region deletion mutants that do not make stable truncated forms of T antigen. Since all five of these mutants have intact early- and late-region control sequences, we conclude that these control sequences are not sufficient for immortalization. Three of the mutants that did not immortalize did make a normal small t antigen, suggesting that the expression of this protein alone is not sufficient for immortalization of early-passage Fisher rat embryo cells.
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D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics; D001483:Base Sequence; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics*; D003673:Defective Viruses / Q000235:genetics; D005814:Genes, Viral; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics*
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral