title: Possible applications of recombinant DNA technology to the diagnosis and study of variants of alpha 1-antitrypsin.
abstract: We describe the basic principles of recombinant DNA technology and how this methodology has been used to isolate and characterise cloned genes coding for alpha 1-antitrypsin. We show how these probes can be used for diagnosis and to study molecular variants of alpha 1-antitrypsin which may predispose individuals to develop lung disease.
authors.forename: N
authors.initials: N
authors.lastname: Kalsheker
authors.forename: D
authors.initials: D
authors.lastname: Chiswell
authors.forename: A
authors.initials: A
authors.lastname: Markham
authors.forename: A
authors.initials: A
authors.lastname: Imam
authors.forename: S
authors.initials: S
authors.lastname: Wallis
authors.forename: R
authors.initials: R
authors.lastname: Williamson
authors.forename: S E
authors.initials: SE
authors.lastname: Humphries
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005819:Genetic Markers; D006801:Humans; D008171:Lung Diseases / Q000201:enzymology / Q000235:genetics; D009154:Mutation; D012306:Risk; D012907:Smoking; D000515:alpha 1-Antitrypsin / Q000235:genetics*
chemical_list: D000596:Amino Acids; D004274:DNA, Recombinant; D005819:Genetic Markers; D000515:alpha 1-Antitrypsin; D004247:DNA; D004262:DNA Restriction Enzymes