title: Metabolism of leukotriene B4 in hepatic microsomes.
abstract: Leukotriene B4 was metabolized in rat hepatic microsomes to two products. Mass spectral analysis of these two metabolites indicated that the major metabolite was the 20-hydroxy metabolite while the minor metabolite was the 19-hydroxy metabolite. The formation of these metabolites required NADPH and was linear with time (20 min) and protein (1.6 mg/ml). The Km apparent and Vmax for omega hydroxylation of LTB4 was 14 uM and 0.138 nmol/min/mg protein. In contrast, the km and Vmax for omega minus one hydroxylation was 54 uM and 0.093 nmol/min/mg protein. These results suggest that omega and omega minus one hydroxylations of LTB4 may be mediated by different isozymes of hepatic P-450.
authors.forename: J F
authors.initials: JF
authors.lastname: Newton
authors.forename: R
authors.initials: R
authors.lastname: Eckardt
authors.forename: P E
authors.initials: PE
authors.lastname: Bender
authors.forename: T
authors.initials: T
authors.lastname: Leonard
authors.forename: K
authors.initials: K
authors.lastname: Straub
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D007975:Leukotriene B4 / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D007975:Leukotriene B4