title: Use of egg yolk to determine antibody levels in chickens inoculated with a hemagglutinating duck adenovirus (adenovirus 127-like).
abstract: Chickens were experimentally infected with a duck adenovirus that has been shown to be serologically indistinguishable from Adenovirus 127. Sera and eggs were collected at intervals after exposure for antibody determination by the hemagglutination-inhibition (HI) test, the enzyme-linked immunosorbent assay (ELISA), and the immunodiffusion (ID) test. Egg yolks were processed for use in the serological tests by (a) dilution in phosphate-buffered saline (PBS), (b) extraction of the water-soluble fraction with chloroform, or (c) freezing and thawing PBS-diluted yolks and testing the supernatant fluid. HI antibody titers from serum and extracted yolk were similar except during the initial 2 weeks, when yolk antibody levels were low or absent. Chloroform-extracted yolks were suitable material for the HI, ELISA, and ID tests. Heat inactivation of the chloroform-extracted yolk had no effect on titers.
authors.forename: T H
authors.initials: TH
authors.lastname: Piela
authors.forename: V J
authors.initials: VJ
authors.lastname: Yates
authors.forename: P W
authors.initials: PW
authors.lastname: Chang
mesh_terms: D000256:Adenoviridae / Q000276:immunology*; D000257:Adenoviridae Infections / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000259:Aviadenovirus / Q000276:immunology*; D002645:Chickens; D004372:Ducks / Q000382:microbiology; D004530:Egg Yolk; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D005779:Immunodiffusion / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral