Purified proteins acting on cultured chick embryo ciliary ganglion neurons.
Chick embryo ciliary ganglion neurons in dissociated monolayer culture have been used to examine molecular requirements for neuronal survival and neurite growth. These neurons will rapidly die in vitro unless supplied with an adequate level of ciliary neuronotrophic factor (CNTF), and even in the presence of CNTF they will not vigorously extend neurites on polyornithine substrata unless supplied with appropriate amounts of polyornithine-binding neurite-promoting factors (PNPFs). Recent work on the purification and partial characterization of embryonic chick eye CNTF and rat schwannoma PNPF is reviewed, and in vitro responses of ciliary ganglion neurons to other purified proteins such as laminin, fibronectin, insulin, and nerve growth factor are mentioned.
Manthorpe
M
M
Davis
G E
GE
Varon
S
S
D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D020934:Ciliary Neurotrophic Factor; D004591:Electrophoresis, Polyacrylamide Gel; D005123:Eye / Q000737:chemistry; D005726:Ganglia, Parasympathetic / Q000166:cytology*; D007526:Isoelectric Point; D007797:Laminin / Q000494:pharmacology; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000737:chemistry; D009380:Neoplasms, Nerve Tissue / Q000737:chemistry; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D010455:Peptides / Q000378:metabolism; D051381:Rats; D012583:Schwann Cells / Q000737:chemistry
D002352:Carrier Proteins; D020934:Ciliary Neurotrophic Factor; D007797:Laminin; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D010455:Peptides; C008973:polyornithine