Coupled endo-exodeoxyribonuclease activity from HeLa cell nuclei can function in an excision repair mode in vitro.
A single strand specific coupled endo-exodeoxyribonuclease activity has been purified and characterized from the nuclei of HeLa cells(7). The novelity of this activity is in the nature of its associated endo and exonuclease activities. Due to this aspect the ability of this activity to work in unison with DNA polymerase molecules in the process of DNA repair synthesis was investigated.
Quarless
S A
SA
D000818:Animals; D002417:Cattle; D004247:DNA / Q000528:radiation effects; D004256:DNA Polymerase I / Q000502:physiology; D004257:DNA Polymerase II / Q000502:physiology; D004260:DNA Repair; D004278:DNA, Superhelical / Q000378:metabolism; D004720:Endonucleases / Q000502:physiology*; D005092:Exonucleases / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D011485:Protein Binding; D014466:Ultraviolet Rays
D004278:DNA, Superhelical; D004247:DNA; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004720:Endonucleases; D005092:Exonucleases; C020104:endoexonuclease