title: Expression of the c-sis gene and secretion of a platelet-derived growth factor-like protein by simian virus 40-transformed BHK cells.
abstract: SV40-transformed BHK cells were shown to express two transcripts, of 3.5 kb and 2.0 kb, that hybridised to a human c-sis probe. Antibodies directed against human PDGF specifically recognized a 31 kDa protein in SV40/BHK cell conditioned medium, which upon reduction was split into 16 kDa species. Unfractionated conditioned medium and one of two growth factors isolated from SV40/BHK cells competed with 125I-PDGF for binding to its receptor. The present communication thus provides compelling evidence that an SV40/BHK cell-derived growth factor is a hamster equivalent to human PDGF.
authors.forename: C
authors.initials: C
authors.lastname: Betsholtz
authors.forename: M
authors.initials: M
authors.lastname: Bywater
authors.forename: B
authors.initials: B
authors.lastname: Westermark
authors.forename: R R
authors.initials: RR
authors.lastname: Bürk
authors.forename: C H
authors.initials: CH
authors.lastname: Heldin
mesh_terms: D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D006224:Cricetinae; D005786:Gene Expression Regulation; D005796:Genes; D006133:Growth Substances / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D013539:Simian virus 40
chemical_list: D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor