title: Ultrastructural localization of the herpes simplex virus major DNA-binding protein in the nucleus of infected cells.
abstract: Using immunocytochemical procedures in conjunction with electron microscopy we have examined the distribution of the major DNA-binding protein (DBP) of herpes simplex virus (HSV) in infected nuclei. In embedded specimens, DBP was preferentially associated with fibrillar material of electron-translucent viral inclusions, and to a lesser extent with peripheral (marginated) host chromatin. The latter association was sensitive to a non-ionic detergent ('Photo flo'). In chromatin spread by the Miller technique, DBP was found to be a component of the 10 nm 'thick filaments' previously described in HSV infection.
authors.forename: F
authors.initials: F
authors.lastname: Puvion-Dutilleul
authors.forename: M
authors.initials: M
authors.lastname: Laithier
authors.forename: P
authors.initials: P
authors.lastname: Sheldrick
mesh_terms: D002467:Cell Nucleus / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D018139:Simplexvirus / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins