A scheme for determining the correct activity of the kinetic angiotensin-converting enzyme.
The absorbance difference measured when angiotensin-converting enzyme (EC 3.4.15.1) hydrolyzes the substrate N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine is the basis for measuring its activity. We show this difference to be instrument dependent, and describe a method for deriving it that is applicable to manual or automated procedures.
Buttery
J E
JE
Chamberlain
B R
BR
D001322:Autoanalysis; D006033:Glycylglycine / Q000032:analysis; D007700:Kinetics; D009842:Oligopeptides / Q000032:analysis; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D009842:Oligopeptides; D006033:Glycylglycine; D010649:Phenylalanine; C044911:N-(3-(2-furyl)acryloyl)phenylalanine; C038275:2-furanacryloyl-phenylalanyl-glycyl-glycine; D007703:Peptidyl-Dipeptidase A