title: The large scale purification of ubiquitin from human erythrocytes.
abstract: A simple, reproducible method for the large-scale purification of active ubiquitin from human erythrocytes is described. Erythrocytes contain 100 micrograms free ubiquitin per cc of packed cells, of which 44% can be recovered in homogeneous form by a combination of heat treatment, ammonium sulfate fractionation, and ion exchange chromatography.
authors.forename: A L
authors.initials: AL
authors.lastname: Haas
authors.forename: K D
authors.initials: KD
authors.lastname: Wilkinson
mesh_terms: D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D004912:Erythrocytes / Q000032:analysis*; D006609:High Mobility Group Proteins / Q000097:blood*; D006358:Hot Temperature; D006801:Humans; D014452:Ubiquitins / Q000097:blood*
chemical_list: D006609:High Mobility Group Proteins; D014452:Ubiquitins