Molecular forms of atrial natriuretic peptides in dog atrium and plasma.
The level of immunoreactive ANP (iANP) as determined by radioimmunoassay, exhibits a fairly even distribution throughout the canine atria. However, the maximal concentration was found in the appendages of both the left and right atrium, and the level was significantly higher on the left side in all analyzed localizations. Reverse-phase HPLC of atrial extracts, coupled with RIA, revealed the presence of three iANP fractions. The dominant fraction (65% of iANP) has an estimated molecular weight 16,300, showing that it corresponds to the hormone precursor. By contrast, in the plasma extract, 92% of iANP coelutes with synthetic human ANP99-126, thus confirming the structural identity of dog and human ANP.
Cernacek
P
P
Department of Medicine, McGill University, Royal Victoria Hospital, Montreal, Canada.
Maher
E
E
Crawhall
J C
JC
Levy
M
M
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006325:Heart Atria / Q000032:analysis; D006801:Humans; D046911:Macromolecular Substances; D008970:Molecular Weight; D009206:Myocardium / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis; D011863:Radioimmunoassay; D014018:Tissue Distribution
D046911:Macromolecular Substances; D011498:Protein Precursors; D009320:Atrial Natriuretic Factor