Receptor-mediated uptake of hypertriglyceridaemic VLDL remnants by mouse macrophages in vitro.
Following incubation of the VLDL isolated from hypertriglyceridaemic patients with normal human postheparin plasma VLDL remnants were obtained. 125I-VLDL remnants were taken up and catabolized by mouse macrophages in vitro. This was a receptor-dependent process. Unlabelled VLDL remnants added to the incubation medium inhibited the degradation of 125I-VLDL remnants in the cells. This could not be produced by the presence of VLDL or acetyl-LDL in the medium. It shows that mouse macrophages bind VLDL remnants by means of an other receptor than that for acetyl-LDL. The cell cholesterol content increased after incubation of the cells with VLDL remnants or acetyl-LDL, but not with native VLDL or LDL.
Naruszewicz
M
M
Szostak
W B
WB
D000818:Animals; D002784:Cholesterol / Q000097:blood; D006953:Hyperlipoproteinemia Type IV / Q000097:blood / Q000378:metabolism*; D066298:In Vitro Techniques; D008079:Lipoproteins, VLDL / Q000097:blood*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018110:Receptors, Lipoprotein; D014280:Triglycerides / Q000097:blood*
D008079:Lipoproteins, VLDL; D011956:Receptors, Cell Surface; D018110:Receptors, Lipoprotein; D014280:Triglycerides; D002784:Cholesterol