Three-dimensional structure of renal Na,K-ATPase determined by electron microscopy of membrane crystals.
The three-dimensional structure of Na,K-ATPase was determined by electron microscopy and image processing. Tilt series of negatively stained membrane crystals were recorded. The projections were analyzed by Fourier methods and the data combined to a 3-D model. The unit cell contains two rod-shaped stain-deficient regions interpreted as alpha beta-promoters of Na,K-ATPase. The rods are related by dyad axes oriented perpendicular to the membrane. Outside the lipid bilayer the rods contact different protein units on the two sides of the membrane.
Herbert
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H
Skriver
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E
Maunsbach
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AB
D000818:Animals; D003460:Crystallization; D007679:Kidney Medulla / Q000201:enzymology*; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008956:Models, Chemical; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D000254:Sodium-Potassium-Exchanging ATPase