Structural analysis of a developmentally regulated 25-kDa protein gene of Sarcophaga peregrina.
In the previous paper, we described the identification of two abundant mRNAs of Sarcophaga peregrina (flesh-fly) which are selectively expressed in the fat body of middle third instar larvae. One of these mRNAs was found to encode a protein with a molecular mass of about 25,000 (25-kDa protein) when translated in vitro (Tamura, H., et al. (1983) Dev. Biol. 99, 145-151). Present paper reports the nucleotide sequence of a 2.3 kb DNA containing the entire gene for the 25-kDa protein. This gene consisted of four exons and contained an open reading frame for 184 amino acids. A CAT box and a TATA box were found in the 5'-flanking sequence. A poly A addition signal of AATAAA was assigned to the non-coding region in the fourth exon. A sequence having 75% homology with SV40 enhancer core sequence was identified in the non-coding region of the first exon.
Matsumoto
N
N
Sekimizu
K
K
Soma
G
G
Ohmura
Y
Y
Andoh
T
T
Nakanishi
Y
Y
Obinata
M
M
Natori
S
S
D000273:Adipose Tissue; D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004175:Diptera / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D005796:Genes; D008970:Molecular Weight; D011506:Proteins / Q000235:genetics*
D011506:Proteins; D004262:DNA Restriction Enzymes