title: Rat Sertoli cells acquire a beta-adrenergic response during primary culture.
abstract: Two-dimensional polyacrylamide gel electrophoresis and the radioligand (-)-[125I]iodopindolol (125I-Pin) have been used to study isoproterenol-dependent protein phosphorylation and beta-adrenergic receptor availability, respectively, in cultured Sertoli cells and freshly isolated seminiferous tubular segments of sexually immature and mature rats. Sertoli cells prepared from sexually immature rats show progressive 125I-Pin binding in primary cultures that correlates with isoproterenol-induced cell shape changes, redistribution of immunoreactive vimentin, and phosphorylation of this intermediate filament protein. The development of 125I-Pin binding to Sertoli cell lysates is blocked by cycloheximide. Seminiferous tubules do not show significant isoproterenol-dependent vimentin phosphorylation nor 125I-Pin binding. However, vimentin phosphorylation can be induced by follicle-stimulating hormone or a cyclic nucleotide analog. This study stresses the need for correlating pharmacological-induced responses observed in Sertoli cell primary cultures with those in the intact seminiferous tubule.
authors.forename: A L
authors.initials: AL
authors.lastname: Kierszenbaum
authors.forename: W A
authors.initials: WA
authors.lastname: Spruill
authors.forename: M G
authors.initials: MG
authors.lastname: White
authors.forename: L L
authors.initials: LL
authors.lastname: Tres
authors.forename: J P
authors.initials: JP
authors.lastname: Perkins
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010766:Phosphorylation; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000032:analysis*; D012671:Seminiferous Tubules / Q000378:metabolism; D012708:Sertoli Cells / Q000032:analysis / Q000187:drug effects*
chemical_list: D011506:Proteins; D011943:Receptors, Adrenergic, beta; C029247:3-iodopindolol; D005640:Follicle Stimulating Hormone; D010869:Pindolol; D007545:Isoproterenol