Preparation of a cell-free translation system from a wild-type strain of Neurospora crassa.
We describe the preparation of an in vitro translation system from a wild-type strain of Neurospora crassa. The system is capable of supporting efficient and faithful translation of native and in vitro transcribed eukaryotic messages. The translation products have minimal background and can be clearly analyzed by SDS-polyacrylamide gel electrophoresis. The method of preparation of the lysate is simple, fast and reproducible. The procedure should be readily applicable to other filamentous fungi.
Devchand
M
M
Allelix Inc., Mississauga, Ontario, Canada.
Buxton
F P
FP
Gwynne
D I
DI
Davies
R W
RW
D002474:Cell-Free System; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics*; D011188:Potassium / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D012333:RNA, Messenger; D008274:Magnesium; D011188:Potassium