A commercially available serum-free medium appropriate for studies of macrophage functions in vitro.
This report presents results using a commercially available serum-free medium, HL-1, to study growth and differentiation of the macrophage-like cell line Mm-1. Mm-1 cells grown in RPMI 1640 plus 5% calf serum were compared with the same cells acclimated to and maintained in HL-1. The two medium formulations are compared for their ability to support replication of the line as well as their ability to support the expression of non-specific esterase production, lysozyme secretion, Fc receptor expression, and phagocytosis of latex beads. The serum-supplemented RPMI promoted growth of Mm-1 cells slightly better than HL-1, but the HL-1 medium consistently supported differentiated functions of the cells 8-10% above the level expressed in serum-supplemented RPMI at the same cell density. Based on these findings and the low endotoxin levels of HL-1, we conclude that the medium is appropriate for refined physiological studies of macrophage functions in this cell line.
Majde
J A
JA
Office of Naval Research, Arlington, VA 22217.
D000818:Animals; D000943:Antigens, Differentiation; D001790:Blood Physiological Phenomena; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media / Q000032:analysis / Q000494:pharmacology; D004731:Endotoxins / Q000032:analysis; D008264:Macrophages / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D009113:Muramidase / Q000096:biosynthesis; D010587:Phagocytosis; D011961:Receptors, Fc; D017452:Receptors, IgG
D000943:Antigens, Differentiation; D003470:Culture Media; D004731:Endotoxins; D011961:Receptors, Fc; D017452:Receptors, IgG; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D009113:Muramidase