Location of rep and inc sequences in the F secondary replicon.
Miniplasmids derived by deletion of DNA from the F plasmid secondary replicon have been tested for the ability to replicate and to express incompatibility with the IncFI plasmid, ColV3-K30. The results demonstrate that the minimal rep region of the secondary replicon lies within a 1.9-kb sequence (33.7F-35.6F kb), and that an inc region, presumably involved in replication control, is present in a 0.45-kb portion of the rep region (33.7F-34.15F kb). In addition, the secondary replicon was found not to require DNA polymerase I activity.
Gardner
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McAnulty
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Feher
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Lane
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D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005798:Genes, Bacterial; D010957:Plasmids; D012093:Replicon
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI