Expression of the gene for the atrial natriuretic peptide in cardiac myocytes in vitro.
Primary cultures of neonatal cardiocytes express the gene for atrial natriuretic peptide (ANP). In general the levels of expression follow the rank order: atrial greater than ventricular much greater than nonmyocardial cells. Following the initial dispersion cardiocytes require 48 to 72 hours before ANP secretion and ANP mRNA accumulation approach a new steady-state level. In situ hybridization analysis indicates that ANP gene expression is concentrated in a subpopulation of cardiocytes in both the atrial and the ventricular cell cultures. These findings suggest that these primary cultures may be of value in defining the factors governing the expression of the ANP gene in the cardiac cell.
Gardner
D G
DG
Metabolic Research Unit, University of California, San Francisco 94143.
Wu
J P
JP
LaPointe
M C
MC
Hedges
B K
BK
Deschepper
C F
CF
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D015870:Gene Expression; D009206:Myocardium / Q000378:metabolism*; D051381:Rats
D009320:Atrial Natriuretic Factor