Studies on cytochrome c oxidase, XI. The amino-acid sequence of bovine heart polypeptide VIc.
The complete primary structure of the cytoplasmically synthesized polypeptide VIc from beef heart cytochrome c oxidase was determined via isolation and sequencing of overlapping methionine and glutamic acid fragments. The protein consists of 73 amino acids (Mr 8 480). Through the protein contains, from residues 21 to 40, a hydrophobic sequence interrupted by one lysine it may not penetrate the membrane. A sequence of 33 amino acids highly homologous to the C-terminal part of VIc has been translated from a cDNA clone of a nuclear coded subunit of the enzyme from rat liver. The function of this component of the terminal oxidase is yet unknown.
Erdweg
M
M
Buse
G
G
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D002417:Cattle; D003488:Cyanogen Bromide; D003576:Electron Transport Complex IV / Q000032:analysis* / Q000302:isolation & purification; D010450:Endopeptidases; D006868:Hydrolysis; D008666:Metalloendopeptidases; D009206:Myocardium / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis*; D010455:Peptides / Q000032:analysis
D000596:Amino Acids; D010446:Peptide Fragments; D010455:Peptides; D003576:Electron Transport Complex IV; D002268:Carboxypeptidases; D010450:Endopeptidases; D043422:Carboxypeptidases A; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus; D003488:Cyanogen Bromide