Ethanol and leucine as possible stress factors in rats marginally deficient in niacin and vitamin B-6.
Young rats were fed a purified diet containing no niacin (and 0.85 g/kg tryptophan) and only 0.5 mg/kg vitamin B-6. Three supplements were tested in a 2 x 2 x 2 multifactorial experiment: 1) 25 mg/kg niacin plus 7.3 mg/kg vitamin B-6; 2) 15 g/kg L-leucine; and 3) 12.15% (wt/wt) ethanol in the drinking water. The ethanol, for those receiving it, contributed approximately 15% of the rats' total energy intake, and their diets had some of the carbohydrate omitted to maintain approximately the same proportion of energy to other nutrients. The vitamin supplements stimulated 4-wk weight gain (and gain-feed ratio), but ethanol depressed it, and to a significantly greater extent in the absence of the vitamin supplement. Excretion of N1-methylnicotinamide was increased only by the vitamin supplement. Leucine had no significant effects either by itself or by interaction with the other supplements.
Cook
N E
NE
Department of Nutritional Sciences, University of California, Berkeley 94720.
Carpenter
K J
KJ
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002149:Energy Intake; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D007930:Leucine / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009525:Niacin / Q000008:administration & dosage / Q000172:deficiency*; D009536:Niacinamide / Q000031:analogs & derivatives / Q000652:urine; D010383:Pellagra / Q000209:etiology; D011736:Pyridoxine / Q000008:administration & dosage; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D013312:Stress, Physiological; D026681:Vitamin B 6 Deficiency / Q000150:complications*
D009536:Niacinamide; D009525:Niacin; D000431:Ethanol; D007930:Leucine; D011736:Pyridoxine; C008472:N-methylnicotinamide