Transmission of latent cytomegalovirus in a murine kidney tissue transplantation model.
This paper examines the hypothesis that latent murine cytomegalovirus (MCMV) may be transmitted in kidney tissue to transplant recipients. Balb/c mice were infected with MCMV, and at intervals of less than 1 week to greater than 1 year, transmission of the virus from infected donors was attempted by transplantation of kidney sections or transfusion of blood into uninfected recipients. Graft recipients were killed from 2-4 weeks later, and cultured for MCMV. Restriction endonuclease digestion profiles of viral DNA were performed. Acutely infected donors transmitted MCMV in kidney tissue to 83-66% of untreated, susceptible recipients. Latently infected donors transmitted the infecting strain of virus to 20% of all and 31% of immunosuppressed recipients but to 37% of the syngeneic versus 21% of the allogeneic (P less than .027). Blood transfusions transmitted latent virus to 28% of recipients. In conclusion, kidney tissue may serve as the source of latent virus in this murine transplantation model. Retained blood in the kidney is unlikely to be the only source of virus.
Hamilton
J D
JD
Seaworth
B J
BJ
D000208:Acute Disease; D000818:Animals; D001803:Blood Transfusion; D003587:Cytomegalovirus / Q000254:growth & development / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000382:microbiology / Q000635:transmission*; D004195:Disease Models, Animal; D006183:H-2 Antigens / Q000235:genetics; D006650:Histocompatibility Testing; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007668:Kidney / Q000382:microbiology; D016030:Kidney Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013997:Time Factors; D014019:Tissue Donors; D014775:Virus Activation
D006183:H-2 Antigens; D007166:Immunosuppressive Agents