[A library of genes of plasmid PSS120 of Shigella sonnei].
The gene library of S. sonnei plasmid pSS120 was constructed with the use of plasmid pSL5 as vector. The complete restriction of the vector DNA and the partial restriction of the DNA of plasmid pSS120 were carried out by means of the enzyme EcoRI. The restricted DNA was ligated and packed in vitro into the capsid of phage lambda. The titer of negative colonies obtained after packing was 0.8 X 10(3) clones per 1 microgram of S. sonnei DNA. The total number of detected clones was 250. On the basis of the results, obtained in the analysis of the inserts of the DNA of plasmid pSS120 into the DNA of recombinant clones, the theoretical volume of the library, equal to 92 clones, was calculated. The collection of clones thus obtained will be used for checking the presence of the determinants of invasiveness and phase I antigen, localized in the DNA of plasmid pSS120.
Belova
T S
TS
Romanova
Iu M
IuM
D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005822:Genetic Vectors; D010957:Plasmids; D012764:Shigella sonnei / Q000235:genetics*
Biblioteka genov plazmidy PSS120 Shigella sonnei.