Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced nitroblue tetrazolium reduction in mouse peritoneal macrophages by various tumor promotion inhibitors.
The effects of various inhibitors on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced reduction of nitroblue tetrazolium (NBT) in mouse peritoneal macrophages were investigated. The reduction was inhibited by phospholipase A2 inhibitors, such as dibromoacetophenone, the lipoxygenase inhibitor nordihydroguaiaretic acid, an NADH-dehydrogenase inhibitor, the microfilament inhibitor cytochalasin B, oxygen radical scavengers such as superoxide dismutase, antioxidants such as butyl hydroxyanisole and non-specific inhibitors such as retinoic acid. The reduction was not affected by the cyclooxygenase inhibitor indomethacin or the H2O2 scavenger catalase.
Ohkawa
Y
Y
Iwata
K
K
Shibuya
H
H
Inui
N
N
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors; D005260:Female; D066298:In Vitro Techniques; D016859:Lipoxygenase Inhibitors; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010704:Phorbols / Q000633:toxicity*; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D013778:Tetrazolium Salts / Q000378:metabolism*
D000975:Antioxidants; D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D010704:Phorbols; D013778:Tetrazolium Salts; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase; D009245:NADH Dehydrogenase; D010741:Phospholipases A; D054467:Phospholipases A2; D013755:Tetradecanoylphorbol Acetate