[Activation of adenylate cyclase system enzymes and lysosomal acid phosphatase in target cells interacting with T-killer cells].
The interaction of T-killers with target cells was studied to reveal the biochemical changes in the latter. On specific binding of target cells with T-killers the activity in target cells of cAMP phosphodiesterase increased 2.1-fold, the level of cAMP decreased 1.5-fold, the adenylate cyclase activity decreased 2.0-fold, the phosphorylation of intracellular proteins decreased 1.8-fold, the cAMP-dependent protein kinase activity decreased 1.7-fold. No change in the activity of lysosomal enzymes was observed. At the "independent target cells lysis" stage the level of cAMP increased 1.8-fold, the phosphodiesterase activity decreased 1.7-fold, the cAMP-dependent protein kinase activity increased 1.8-fold, the released activity of acid phosphatase increased up to 40% compared with the control cells. In the presence of 1 mM dibutyryl cAMP the released activity of the acid phosphatase in target cells was inhibited by 29%, the target cells lysis was decreased by 23,5%. The data obtained allowed to suppose that the activation of the host lysosomal enzymes causes target cells autolysis and that cAMP takes part in the regulation of these processes.
Sashchenko
L P
LP
Kupriianova
T A
TA
Memelova
L V
LV
Bykovskaia
S N
SN
Gnuchev
N V
NV
D000135:Acid Phosphatase / Q000378:metabolism*; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000201:enzymology / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D007739:L Cells / Q000201:enzymology* / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008247:Lysosomes / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology
D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP; D011494:Protein Kinases; D000135:Acid Phosphatase; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases; D008274:Magnesium
Aktivatsiia fermentov adenilattsiklaznoĭ sistemy i lizosomnoĭ kisloĭ fosfatazy v kletkakh-misheniakh, vzaimodeĭstvuiushchikh s T-killerami.