Presence of atrial natriuretic peptide-like material in guinea pig intestine.
Acidic extracts of guinea pig jejunum and colon contain atrial natriuretic peptide-like material (IR-ANP) detected by radioimmunoassay after purification by Sephadex G-50 gel filtration. Immunohistochemical analysis of guinea pig colon also revealed IR-ANP to be located directly beneath the lamina muscularis mucosae. High performance gel permeation chromatography (HP-GPC) and reverse phase high performance chromatography (RP-HPLC) of the IR-ANP showed correspondence to the 15 kD ANP precursor molecule (pro-ANP). No low molecular weight forms of ANP were detected. The extracted pro-ANP could be converted to alpha-ANP-like material by incubation with serum or supernatant of colonic homogenate. These data indicate the intestine to be a further site of ANP synthesis.
Vollmar
A M
AM
Institut für Pharmakologie, Toxikologie und Pharmazie, Universität Mäunchen, FRG.
Friedrich
A
A
Sinowatz
F
F
Schulz
R
R
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000032:analysis* / Q000166:cytology; D006168:Guinea Pigs; D007583:Jejunum / Q000032:analysis* / Q000166:cytology; D008297:Male; D011863:Radioimmunoassay / Q000379:methods
D009320:Atrial Natriuretic Factor