title: [Isolation and properties of 2 forms of cyclic nucleotide phosphodiesterase from the rat brain under normal conditions and after irradiation].
abstract: It is established that the functional activity of two phosphodiesterase forms--phosphodiesterase I (Ca2+-calmodulin-sensitive) and phosphodiesterase II (Ca2+-calmodulin-insensitive), isolated from grey matter of the irradiated rat brain varies essentially in comparison with that of the normal rats. In the early period of acute radiation injury both phosphodiesterase I sensitivity to calmodulin and phosphodiesterase II special activity under hydrolysis of 3', 5'-GMP decrease but phosphodiesterase I special activity under hydrolysis of 3', 5'-GMP increases. The investigation of temperature dependence of phosphodiesterase I and phosphodiesterase II activations revealed changes in character of curves, the temperature optimum under irradiation being unchanged and inflections appearing on the Arrhenius curves.
authors.forename: T I
authors.initials: TI
authors.lastname: Parkhomets
authors.forename: A N
authors.initials: AN
authors.lastname: Vasil'ev
authors.forename: S N
authors.initials: SN
authors.lastname: Chernen'kaia
authors.forename: N E
authors.initials: NE
authors.lastname: Kucherenko
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002147:Calmodulin / Q000378:metabolism; D005260:Female; D006157:Guanosine Monophosphate / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008297:Male; D011833:Radiation Injuries, Experimental / Q000201:enzymology*; D051381:Rats; D013696:Temperature
chemical_list: D002147:Calmodulin; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases
vernacular_title: Vydelenie i svoĭstva dvukh form fosfodiésterazy tsiklicheskikh nukleotidov iz golovnogo mosga krys v norme i pri obluchenii.