The structure and evolution of a 461 amino acid human protein C precursor and its messenger RNA, based upon the DNA sequence of cloned human liver cDNAs.
Human liver cDNA coding for protein C has been synthesized, cloned and sequenced. The abundance of protein C message is approximately 0.02% of total mRNA. Three overlapping clones contain 1,798 nucleotides of contiguous sequence, which approximates the size of the protein's mRNA, based upon Northern hybridization. The cDNA sequence consists of 73 5'-noncoding bases, coding sequence for a 461 amino acid nascent polypeptide precursor, a TAA termination codon, 296 3'-noncoding bases, and a 38 base polyadenylation segment. The nascent protein consists of a 33 amino acid "signal", a 9 amino acid propeptide, a 155 amino acid "light" chain, a Lys-Arg connecting dipeptide, and a 262 amino acid "heavy" chain. Human protein C and Factor IX and X precursors possess about one third identical amino acids (59% in the gamma-carboxyglutamate domain), including two forty-six amino acid segments homologous to epidermal growth factor. Human protein C also has similar homology with prothrombin in the "leader", gamma-carboxyglutamate and serine protease domains, but lacks the two "kringle" domains found in prothrombin.
Beckmann
R J
RJ
Schmidt
R J
RJ
Santerre
R F
RF
Plutzky
J
J
Crabtree
G R
GR
Long
G L
GL
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D005164:Factor IX / Q000032:analysis; D005170:Factor X / Q000032:analysis; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011486:Protein C; D011498:Protein Precursors / Q000235:genetics*; D011516:Prothrombin / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*
D006023:Glycoproteins; D011486:Protein C; D011498:Protein Precursors; D012333:RNA, Messenger; D011516:Prothrombin; D005164:Factor IX; D005170:Factor X; D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases; C029792:GTCGAC-specific type II deoxyribonucleases