[The mixed lympho-epidermal culture: a predictive test of the graft-versus-host reaction in receivers of bone marrow grafts].
Mixed lymphocyte reactions (MLR) and mixed epidermal cell-lymphocyte reactions (MECLR) were performed concurrently before grafting in patients scheduled to receive a bone marrow graft from an HLA-identical sibling for an hematological malignancy. For each donor-recipient pair, the ratio of the counts per minute (cpm) for MECLR to the cpm for MLR was calculated. This ratio was significantly higher in the group of patients who subsequently developed grade II to IV graft-vs-host (GVH) disease as compared to the group who had grade O-I GVH. Statistical analysis showed that this parameter was the most significant risk factor for acute GVH, as it was the leading factor selected by stepwise discriminant analysis. The other significant risk factors for acute GVH were previous pregnancies in female donors and chronic myeloid leukemia in recipients. Thus, these parameters allow more accurate evaluation of the risk for GVH in individual recipients before grafting and therefore of the indications for T-cell depletion.
Bagot
M
M
Service de Dermatologie, Hôpital Henri Mondor, Créteil, France.
D016026:Bone Marrow Transplantation; D000078404:Epidermal Cells; D006087:Graft vs Host Reaction; D006801:Humans; D007938:Leukemia / Q000473:pathology / Q000628:therapy*; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D009367:Neoplasm Staging; D011237:Predictive Value of Tests
La culture mixte lympho-épidermique: un test prédictif de la réaction du greffon contre l'hôte chez les receveurs de greffes de moelle.