Bacteriophage lambda DNA packaging. The product of the FI gene promotes the incorporation of the prohead to the DNA-terminase complex.
Lambda DNA packaging in vitro can be examined in stages. In a first step, lambda DNA interacts with terminase to form a DNA-enzyme complex, called complex I. Upon addition of proheads, in a second step, a ternary complex, complex II, containing DNA, terminase and the prohead is formed. Finally, upon addition of the rest of the morphogenetic components, complete phages are assembled. We have investigated the effect of the FI gene product (gpFI) in these reactions and found that a stimulation in phage yield is observed when gpFI is included early in the reaction, at the time when DNA, terminase and proheads interact to form complex II. Measurements of complex II formation revealed that gpFI stimulated the rate of formation of this intermediate. gpFI was further shown to stimulate the addition of proheads to preformed complexes I to give complex II, but the protein did not stimulate complex I formation.
Becker
A
A
Department of Medical Genetics, University of Toronto, Ontario, Canada.
Murialdo
H
H
Lucko
H
H
Morell
J
J
D010582:Bacteriophage lambda / Q000502:physiology*; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D005814:Genes, Viral; D007700:Kinetics; D010957:Plasmids; D014176:Protein Biosynthesis; D014764:Viral Proteins / Q000378:metabolism
D004279:DNA, Viral; D014764:Viral Proteins; D004706:Endodeoxyribonucleases; C030894:terminase