Isotypic exclusion of gamma delta T cell receptors in transgenic mice bearing a rearranged beta-chain gene.
The rearrangement of T cell antigen receptor beta- and gamma-chain gene segments was studied in transgenic mice that bear a functional beta-chain gene. Virtually all CD3-positive T cells derived from transgenic mice express beta chains containing the transgene-encoded V beta 8.2 variable region on their surfaces and do not express endogenous beta-chain variable regions. Expression of endogenous V beta genes is inhibited at the level of somatic recombination during thymic ontogeny. Furthermore, rearrangements of the TCR gamma-chain genes are also markedly inhibited in these transgenic animals. Hence expression of the TCR beta transgene has led to allelic exclusion of alpha beta receptors and isotypic exclusion of gamma delta T cell receptors.
Fenton
R G
RG
Department of Genetics, Harvard Medical School, Boston, MA 02115.
Marrack
P
P
Kappler
J W
JW
Kanagawa
O
O
Seidman
J G
JG
D000483:Alleles; D000818:Animals; D000911:Antibodies, Monoclonal; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D007118:Immunoassay; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008099:Liver / Q000032:analysis; D051379:Mice; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000378:metabolism; D013950:Thymus Gland / Q000032:analysis
D000911:Antibodies, Monoclonal; D004274:DNA, Recombinant; D007143:Immunoglobulin Heavy Chains; D011948:Receptors, Antigen, T-Cell; D010047:Ovalbumin