title: Location of rep and inc sequences in the F secondary replicon.
abstract: Miniplasmids derived by deletion of DNA from the F plasmid secondary replicon have been tested for the ability to replicate and to express incompatibility with the IncFI plasmid, ColV3-K30. The results demonstrate that the minimal rep region of the secondary replicon lies within a 1.9-kb sequence (33.7F-35.6F kb), and that an inc region, presumably involved in replication control, is present in a 0.45-kb portion of the rep region (33.7F-34.15F kb). In addition, the secondary replicon was found not to require DNA polymerase I activity.
authors.forename: R
authors.initials: R
authors.lastname: Gardner
authors.forename: J
authors.initials: J
authors.lastname: McAnulty
authors.forename: E
authors.initials: E
authors.lastname: Feher
authors.forename: D
authors.initials: D
authors.lastname: Lane
mesh_terms: D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005798:Genes, Bacterial; D010957:Plasmids; D012093:Replicon
chemical_list: D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI