Effects of TRIS and HEPES on function of rabbit muscle light sarcoplasmic reticulum.
ATP hydrolysis activity and calcium transport activity were determined on light sarcoplasmic reticulum from rabbit skeletal muscle. The effects of two buffers, TRIS and HEPES, were compared. Titration of TRIS into sarcoplasmic reticulum preparations in HEPES provided evidence for TRIS inhibition of ATPase activity and TRIS stimulation of calcium transport activity.
Selinsky
B S
BS
Department of Biochemistry, School of Medicine, University of Buffalo, SUNY 14214.
Messana
A D
AD
Scherer
W
W
Yeagle
P L
PL
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D006531:HEPES / Q000494:pharmacology*; D066298:In Vitro Techniques; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D014325:Tromethamine / Q000494:pharmacology*
D010879:Piperazines; D014325:Tromethamine; D000255:Adenosine Triphosphate; D000252:Calcium-Transporting ATPases; D006531:HEPES; D002118:Calcium