Biochemical effects of gossypol in isolated mitochondria: monovalent cations and ATPase activity.
1. The effect of gossypol acetic acid upon the energy-producing system of isolated liver mitochondria was studied. Gossypol was found to exert an uncoupling effect. 2. Oxygen consumption and ATPase activity were stimulated only when a monovalent alkali metal cations was present. 3. The mitochondrial proton gradient, produced by the enzymatic hydrolysis of ATP, was lower in the gossypol-treated mitochondria than in the control experiments. 4. It is proposed that gossypol stimulates oxygen consumption and the ATPase activity in mitochondria by a protonophoretic effect and a cation translocation process.
Martínez
F
F
Departamento de Bioquímica, Facultad de Medicina, México, D.F. México.
Gamboa
S
S
Díaz-Sánchez
V
V
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002414:Cations, Monovalent; D004734:Energy Metabolism / Q000187:drug effects*; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008094:Lithium / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012413:Rubidium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D014475:Uncoupling Agents
D002414:Cations, Monovalent; D014475:Uncoupling Agents; D000255:Adenosine Triphosphate; D008094:Lithium; D012964:Sodium; D000251:Adenosine Triphosphatases; D006072:Gossypol; D012413:Rubidium; D011188:Potassium; C028178:gossypol acetic acid