title: Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W.
abstract: The aspA gene of Escherichia coli W which encodes aspartase was cloned into the plasmid vector pBR322. The nucleotide sequences of aspA and its flanking regions were determined. The aspA gene encodes a protein with a molecular weight of 52,224 consisted of 477 amino acid residues. The amino acid sequence of the protein predicted from the nucleotide sequence was consistent with those of the NH2- and COOH-terminal regions and also with the amino acid composition of the purified aspartase determined previously. Potential promoter and terminator sequences for aspA were also found in the determined sequence.
authors.forename: J S
authors.initials: JS
authors.lastname: Takagi
authors.forename: N
authors.initials: N
authors.lastname: Ida
authors.forename: M
authors.initials: M
authors.lastname: Tokushige
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Shimura
mesh_terms: D000595:Amino Acid Sequence; D000642:Ammonia-Lyases / Q000235:genetics*; D001220:Aspartate Ammonia-Lyase / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008040:Genetic Linkage; D011401:Promoter Regions, Genetic
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D000642:Ammonia-Lyases; D001220:Aspartate Ammonia-Lyase