title: Guinea-pig liver testosterone 17 beta-dehydrogenase (NADP+) and aldehyde reductase exhibit benzene dihydrodiol dehydrogenase activity.
abstract: We have kinetically and immunologically demonstrated that testosterone 17 beta-dehydrogenase (NADP+) isoenzymes (EC 1.1.1.64) and aldehyde reductase (EC 1.1.1.2) from guinea-pig liver catalyse the oxidation of benzene dihydrodiol (trans-1,2-dihydroxycyclohexa-3,5-diene) to catechol. One isoenzyme of testosterone 17 beta-dehydrogenase, which has specificity for 5 beta-androstanes, oxidized benzene dihydrodiol at a 3-fold higher rate than 5 beta-dihydrotestosterone, and showed a more than 4-fold higher affinity for benzene dihydrodiol and Vmax. value than did another isoenzyme, which exhibits specificity for 5 alpha-androstanes, and aldehyde reductase. Immunoprecipitation of guinea-pig liver cytosol with antisera against the testosterone 17 beta-dehydrogenase isoenzymes and aldehyde reductase indicated that most of the benzene dihydrodiol dehydrogenase activity in the tissue is due to testosterone 17 beta-dehydrogenase.
authors.forename: A
authors.initials: A
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Hayashibara
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: K
authors.initials: K
authors.lastname: Hasebe
authors.forename: S
authors.initials: S
authors.lastname: Usui
authors.forename: H
authors.initials: H
authors.lastname: Sawada
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000276:immunology / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000276:immunology / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D006168:Guinea Pigs; D007527:Isoenzymes / Q000276:immunology / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010088:Oxidoreductases / Q000276:immunology / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism
chemical_list: D000438:Alcohols; D007527:Isoenzymes; D013256:Steroids; D010088:Oxidoreductases; D015067:17-Hydroxysteroid Dehydrogenases; D000429:Alcohol Oxidoreductases; C019642:alcohol dehydrogenase (NADP+); C032690:3-alpha-(17-beta)-hydroxysteroid dehydrogenase (NAD(+)); C048338:trans-1,2-dihydrobenzene-1,2-diol dehydrogenase