Inhibition of hamster sperm Na+, K+-ATPase activity by taurine and hypotaurine.
Taurine, hypotaurine and the structural analogue, beta-alanine, were tested for their effects on Na+, K+-ATPase activity of crude homogenates prepared from washed cauda epididymal hamster sperm. Preincubation with 0.1-10 mM taurine or hypotaurine inhibited Na+, K+-ATPase in a dose-dependent manner, while beta-alanine had an inhibitory effect only at 10 mM. The results of this study are the first evidence to demonstrate inhibition of Na+, K+-ATPase activity by taurine and hypotaurine and are discussed in relation to the ability of these compounds to sustain hamster sperm motility and fertility.
Mrsny
R J
RJ
Meizel
S
S
D000818:Animals; D006224:Cricetinae; D008297:Male; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013094:Spermatozoa / Q000187:drug effects / Q000201:enzymology*; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D015091:beta-Alanine / Q000494:pharmacology
D015091:beta-Alanine; D013654:Taurine; C003949:hypotaurine; D000254:Sodium-Potassium-Exchanging ATPase