Drosophila P element-enhanced transfection in mammalian cells.
We constructed a gene transfer vector containing the herpes simplex virus type 1 thymidine kinase (TK) gene flanked by Drosophila P element terminal repeats (W. R. Engels, Annu. Rev. Genet. 17:315-344). This vector was introduced into mouse LTK- cells and enhanced the frequency of stable transformation to the TK+ phenotype by approximately 50-fold relative to a similar plasmid lacking the P element terminal repeats.
Clough
D W
DW
Lepinske
H M
HM
Davidson
R L
RL
Storti
R V
RV
D000818:Animals; D002460:Cell Line; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005822:Genetic Vectors; D018139:Simplexvirus / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection
D004251:DNA Transposable Elements; D013937:Thymidine Kinase