title: In vitro recombination of bacteriophage T7 DNA: further characterization of the reaction using plasmid DNA.
abstract: We have used a plasmid which contains a cloned fragment of T7 DNA to study the properties of general recombination of phage T7 in vitro. It was shown that T7-infected cell extracts promote recombination by the exchange of double strands of DNA. While both products of these double-strand exchanges were detected, we were unable to show that they were formed during a single recombination event.
authors.forename: D
authors.initials: D
authors.lastname: Lee
authors.forename: D
authors.initials: D
authors.lastname: Vetter
authors.forename: L
authors.initials: L
authors.lastname: Beatty
authors.forename: P
authors.initials: P
authors.lastname: Sadowski
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic; D013604:T-Phages / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes