Auxin-binding protein from coleoptile membranes of corn (Zea mays L.). II. Localization of a putative auxin receptor.
With the aid of affinity chromatography on auxin-binding protein-Sepharose (ABP-Sepharose) monospecific IgGanti-ABP from rabbit antisera were isolated as judged by immuno-double diffusion test and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. With this IgGanti-ABP the ABP is localized within the outer epidermal cells of coleoptiles using indirect immunofluorescence labeling. Auxin-induced growth of coleoptile segments can be inhibited by IgGanti-ABP, and the auxin response of split coleoptile sections is also strongly reduced by IgGanti-ABP. The ABP, therefore, is referred to as an auxin receptor. This auxin receptor is localized at the plasmalemma of the outer epidermal cells of the coleoptile.
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D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007074:Immunoglobulin G; D010937:Plant Growth Regulators; D010940:Plant Proteins / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000276:immunology / Q000302:isolation & purification*; D003313:Zea mays
D007074:Immunoglobulin G; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C046131:auxin receptor, plant