Direct cloning of the trxB gene that encodes thioredoxin reductase.
A strain was constructed which contains mutations in the genes encoding thioredoxin (trxA) and thioredoxin reductase (trxB) such that filamentous phage f1 cannot grow. The complementation of either mutation with its wild-type allele permits phage growth. We used this strain to select f1 phage which contain a cloned trxB gene. The location of the gene on the cloned fragment was determined, and its protein product was identified. Plasmid subclones that contain this gene overproduce thioredoxin reductase.
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D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics*; D010957:Plasmids; D013880:Thioredoxin-Disulfide Reductase / Q000235:genetics*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D009247:NADH, NADPH Oxidoreductases; D013880:Thioredoxin-Disulfide Reductase; D004262:DNA Restriction Enzymes