Defective myo-inositol-1-phosphate synthase production in an inositolless double mutant Neurospora crassa strain.
A slow growing inl+/- mutant was isolated from an inositol dependent (inl) Neurospora crassa strain. The latter strain produces defective myo-inositol-1-phosphate synthase which has residual activity. Inositol, similarly to that found in wild and inl mutant strains, represses the enzyme production in the inl+/- strain as well. Withdrawing inositol from the medium results in derepression of the enzyme synthesis. Derepression is hindered by cycloheximide. Inl+/- character in the double mutant is brought about by overproduction of the defective myo-inositol-1-phosphate synthase.
Kiss
A
A
Department of Biochemistry, University Medical School of Debrecen, Hungary.
Schablik
M
M
Zsindely
A
A
Szabó
G
G
D002238:Carbohydrate Epimerases / Q000235:genetics*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D007122:Immunoelectrophoresis; D007296:Myo-Inositol-1-Phosphate Synthase / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D013997:Time Factors
D003513:Cycloheximide; D002238:Carbohydrate Epimerases; D007296:Myo-Inositol-1-Phosphate Synthase