title: Proton-coupled transport of glycylglycine in rabbit renal brush-border membrane vesicles.
abstract: Transport of glycylglycine into rabbit renal brush-border membrane vesicles was found to be Na+-independent, H+ gradient-dependent and electrogenic. Marked overshoot uptake of the dipeptide was observed when an inward-directed proton gradient and inside-negative potential difference were imposed simultaneously across the vesicular membranes. Saturable depolarization of vesicular membranes could be demonstrated with glycylglycine by use of a fluorescent cyanine dye, di-S-C3(5). The results indicate that glycylglycine is contransported with H+ across the membranes.
authors.forename: N
authors.initials: N
authors.lastname: Takuwa
authors.forename: T
authors.initials: T
authors.lastname: Shimada
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Hoshi
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004151:Dipeptides / Q000378:metabolism*; D006033:Glycylglycine / Q000378:metabolism*; D007672:Kidney Cortex / Q000648:ultrastructure*; D008564:Membrane Potentials; D008871:Microvilli / Q000378:metabolism; D011522:Protons; D011817:Rabbits; D013997:Time Factors; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D004151:Dipeptides; D011522:Protons; D006033:Glycylglycine; D014634:Valinomycin; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone