Storage of spheroplasts at -70 degrees C for transfection with phi X174 RF DNA.
A method was developed for preparation of spheroplasts used for transfection with phi X174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at -70 degrees C.
Malling
H V
HV
National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Fater
M C
MC
Burkhart
J G
JG
Hardies
S C
SC
Hutchison
C A
CA
Edgell
M H
MH
D010584:Bacteriophage phi X 174 / Q000235:genetics*; D002458:Cell Fractionation; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D005615:Freezing; D013104:Spheroplasts / Q000648:ultrastructure*; D014162:Transfection
D004279:DNA, Viral