Characterization of a Q subregion gene in the murine major histocompatibility complex.
We have used restriction enzyme digests, Southern blot analysis, and gene transfer experiments to identify a class I gene in the Q subregion of the murine major histocompatibility complex. By comparisons of class I genes from Q congeneic strains, five restriction fragment length polymorphisms were identified. Further studies of mutant (Qa-2-) and wild-type (Qa-2+) BALB/c sublines indicated that at least part of the structural or regulatory gene controlling a Q subregion antigen resides on a 3.7-kilobase Xba I DNA fragment and is absent in all tested Qa-2- strains. The spontaneously occurring Qa-2- BALB/cBy mutant appears to have an extensive deletion in this region. The identity of this gene was confirmed by gene transfer experiments as well as by the use of a single-copy probe.
Flaherty
L
L
DiBiase
K
K
Lynes
M A
MA
Seidman
J G
JG
Weinberger
O
O
Rinchik
E M
EM
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I; D008285:Major Histocompatibility Complex; D051379:Mice; D014162:Transfection
D000954:Antigens, Surface; D015395:Histocompatibility Antigens Class I; C032907:Q surface antigens; D004262:DNA Restriction Enzymes