Glycosphingolipids of leukemic cells in adult T-cell leukemia-lymphoma.
We analyzed lipids from leukemic cells of two patients with adult T-cell leukemia and compared them with those from T-cell lymphocytes of normal subjects. The neutral glycosphingolipids and gangliosides which were isolated were characterized by thin-layer chromatography and neuraminidase treatment. Both leukemic cells and normal lymphocytes had monoglycosylceramide and diglycosylceramide as major neutral glycosphingolipids. In one patient, diglycosylceramide was markedly increased. II3NeuAc-LacCer (GM3) and more complex gangliosides were detected in both cells. The most characteristic finding in leukemic cells was the occurrence of a disialylated ganglioside, II3(NeuAc)2-LacCer (GD3), which is not found in normal lymphocytes and neutrophils. This ganglioside may be due to the induced synthesis in association with malignant transformation.
Kuriyama
M
M
Nomura
K
K
Tara
M
M
Matsubara
H
H
Igata
A
A
D002462:Cell Membrane / Q000378:metabolism; D002855:Chromatography, Thin Layer; D017977:Deltaretrovirus; D005732:Gangliosides / Q000378:metabolism; D006028:Glycosphingolipids / Q000378:metabolism*; D006801:Humans; D012192:Retroviridae Infections / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D005732:Gangliosides; D006028:Glycosphingolipids