Constant-pressure perfusion on the isolated rat liver: local oxygen supply and metabolic function.
Isolated rat livers were perfused with a haemoglobin-free Krebs-Ringer solution containing 35 g albumin/1. During a 3 hour perfusion total flow, O2-uptake rate and local O2-supply remained constant. During the same period of time the ATP-content increased from a value of 0.59 +/- 0.17 mumol/g to 1.81 +/- 0.42 mumol/g. The low initial value was due to the ischaemic period during preparation of the organ. The glycogen content increased by 13.4 mg/g, indicating that the organ did not lose the ability for glyconeogenesis. In addition to an increase in glycogen, there was a continuous increase in the glucose concentration of the perfusate, while lactate was taken up by the organ. The results indicate that the isolated organ can be perfused for at least 3 hours without development of disturbances in local O2-supply.
Höper
J
J
Institut für Physiologie und Kardiologie, Universität Erlangen-Nürnberg, FRG.
Kessler
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D009924:Organ Culture Techniques; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D010477:Perfusion; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013047:Specific Pathogen-Free Organisms
D007773:Lactates; D011773:Pyruvates; D000255:Adenosine Triphosphate; D006003:Glycogen; D005947:Glucose; D010100:Oxygen