title: Isolation and characterization of cDNA clones for human liver fibronectin.
abstract: Cellular and plasma fibronectin dimers are constituted by similar but not identical polypeptides. Their differences are the consequence of internal primary sequence variability due to alternative splicing in at least 2 regions (ED and IIICS) of the pre mRNAs [1-8]. A detailed analysis of human liver fibronectin mRNA in these regions was carried out by isolating cDNA clones and determining their nucleotide sequence. A novel type of IIICS segment (coding for 64 amino acids) was present in the two cDNA clones studied and, as expected from previous S1 mapping studies [6], the ED segment was absent in both.
authors.forename: K
authors.initials: K
authors.lastname: Umezawa
authors.forename: A R
authors.initials: AR
authors.lastname: Kornblihtt
authors.forename: F E
authors.initials: FE
authors.lastname: Baralle
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D005353:Fibronectins / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000032:analysis*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D005353:Fibronectins; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes