title: Comparison of the chemotactic response to conditioned medium of BALB/c3T3 fibroblasts and their SV 40 transformants.
abstract: The chemotactic migration of transformed cells out of their tissue of origin might represent an essential component of tumor invasion and metastasis. In the Boyden chamber assay SV 40 virus-transformed BALB-c3T3 fibroblast (SV/3T3) showed an increased chemotactic response to fibroblasts-conditioned medium in comparison to non-transformed BALB/c3T3 cells. This was verified for a large range of incubation times and cell densities. Fibroblast-conditioned medium contains components of the extracellular matrix of connective tissue. Since organs rich in connective tissue are often the site of metastasis, the enhanced chemotaxis of virus-transformed cells to conditioned medium could contribute to explain the malignancy of these cells.
authors.forename: A
authors.initials: A
authors.lastname: Albini
authors.forename: G
authors.initials: G
authors.lastname: Allavena
authors.forename: S
authors.initials: S
authors.lastname: Parodi
authors.forename: L
authors.initials: L
authors.lastname: Santi
mesh_terms: D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002472:Cell Transformation, Viral; D002633:Chemotaxis; D003470:Culture Media; D005347:Fibroblasts / Q000473:pathology; D005353:Fibronectins / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D013539:Simian virus 40; D013997:Time Factors
chemical_list: D003470:Culture Media; D005353:Fibronectins