[Synthesis of visual rhodopsin in a cell-free translation system. I. Influence of the structure of the synthetic bovine visual opsin mRNA on its translational efficiency].
Influence of structural changes in nontranslated regions and translation initiation site of the in vitro synthesized bovine opsin mRNA on its translational efficiency in the wheat germ cell-free system has been studied. It is shown that level of the opsin synthesis up to 30 micrograms per 1 ml of translational mixture can be attained by optimizing structure of 5'-nontranslated region.
Zozulia
S A
SA
Gurevich
V V
VV
Shmukler
B E
BE
Namochin
M Iu
MIu
Zviaga
T A
TA
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002474:Cell-Free System; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides / Q000235:genetics; D010957:Plasmids; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012168:Retinal Pigments / Q000096:biosynthesis* / Q000235:genetics*; D012243:Rhodopsin / Q000096:biosynthesis*; D017299:Rod Opsins; D014158:Transcription, Genetic
D005136:Eye Proteins; D009841:Oligonucleotides; D012333:RNA, Messenger; D012168:Retinal Pigments; D017299:Rod Opsins; D004247:DNA; D012243:Rhodopsin
Sintez zritel'nogo rodopsina v beskletochnoĭ sisteme transliatsii. I. Vliianie struktury sinteticheskoĭ mRNK zritel'nogo opsina byka na éffektivnost' ee transliatsii.