Development and characterization of a human ovarian cancer cell line which clones without agar in vitro.
A new human ovarian cancer cell line has been initiated which clones without agar in vitro. The cell line has been characterized by growth of a tumor resembling the primary tumor in a nude mouse, by human lactic dehydrogenase isozyme pattern, by human karyotype, and by lack of contamination by other cell lines. Initial studies have demonstrated the presence of epidermal growth factor receptors in this cell line. The utility of this line for clonogenic studies is demonstrated by dose-response studies with doxorubicin, cisplatinum, and 13-cis-retinoic acid.
Clamon
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Loh
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White
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Robinson
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D000362:Agar; D000368:Aged; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002291:Carcinoma, Papillary / Q000032:analysis / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D066246:ErbB Receptors; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000032:analysis / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000032:analysis; D014410:Tumor Stem Cell Assay
D000970:Antineoplastic Agents; D007527:Isoenzymes; D011956:Receptors, Cell Surface; D000362:Agar; D066246:ErbB Receptors