title: Induction of post-aggregative differentiation in Dictyostelium discoideum by cAMP. Evidence of involvement of the cell surface cAMP receptor.
abstract: Exogenous cAMP is known to induce post-aggregative differentiation in Dictyostelium discoideum under conditions that normal development is blocked. We have analysed the cyclic nucleotide specificity, the effect of modulation of the cAMP signal and the dose-response relationship of the induction of two independent markers of post-aggregative differentiation, i.e., a prespore cell-specific antigen detected by a monoclonal antibody, and the activity of glycogen phosphorylase. Our results confirm that high concentrations of cAMP (10(-6)-10(-3)M) are required for the induction of these markers. The cells are shown not to adapt to the cAMP signal. The cyclic nucleotide specificity of induction agrees with the specificity of the cell surface cAMP receptor, but is very dissimilar to the specificity of the intracellular cAMP-dependent protein kinase. It is thus unlikely that cAMP leaks into the cell and activates the cAMP-dependent protein kinase directly. Instead, the induction of post-aggregative differentiation by cAMP seems to be mediated by cell surface cAMP receptors.
authors.forename: P
authors.initials: P
authors.lastname: Schaap
authors.forename: R V
authors.initials: RV
authors.lastname: van Driel
mesh_terms: D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D002462:Cell Membrane / Q000502:physiology; D000242:Cyclic AMP / Q000494:pharmacology*; D004023:Dictyostelium / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D006005:Phosphorylases / Q000378:metabolism; D011953:Receptors, Cyclic AMP / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP; D006005:Phosphorylases