title: Cultured Sertoli cell-mediated FSH stimulatory effect on Leydig cell steroidogenesis.
abstract: To determine the precise role of Sertoli cells in the stimulating effects of follicle stimulating hormone (FSH) on Leydig cell activity, porcine purified Leydig and Sertoli cells were cultured separately or together in a chemically defined medium in the absence or presence of porcine, FSH 50 ng/ml. Leydig cell activity was evaluated using two parameters: human chorionic gonadotropin (hCG) binding sites; and hCG-stimulated cAMP production and testosterone secretion. First, it was found that FSH increases Leydig cell activity in crude Leydig cell preparations (40-60% of Leydig cells), whereas it exerts no effect on purified Leydig cells (greater than 90% of Leydig cells). Second, FSH stimulates the activity of Leydig cells cocultured with Sertoli cells, whereas it remains without effect on purified Leydig cells cultured alone. This stimulating effect of FSH on Leydig cell activity is dependent on the Sertoli cell number in the coculture. These data 1) show that the stimulating effect of FSH on Leydig cell function is mediated by Sertoli cells and 2) support the concept of local control of Leydig cell function originating from Sertoli cells.
authors.forename: M
authors.initials: M
authors.lastname: Benahmed
authors.forename: J
authors.initials: J
authors.lastname: Reventos
authors.forename: E
authors.initials: E
authors.lastname: Tabone
authors.forename: J M
authors.initials: JM
authors.lastname: Saez
mesh_terms: D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis; D005640:Follicle Stimulating Hormone / Q000494:pharmacology / Q000502:physiology*; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D012708:Sertoli Cells / Q000502:physiology*; D013268:Stimulation, Chemical; D013552:Swine; D013739:Testosterone / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D013739:Testosterone; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP