Isolation and characterization of a human T cell leukemia virus type II from a hemophilia-A patient with pancytopenia.
Human T cell leukemia virus (HTLV) type I has been isolated from the cultured T cells of several patients with adult T cell leukemia (ATL) and has been etiologically linked to ATL. However, HTLV-type II has been isolated only once, from the T cells of a patient with a T cell variant of hairy-cell leukemia. We report here the isolation of HTLV-II-related virus from the cultured T cells of a hemophilia-A patient with pancytopenia. The T cell line (CM) grows in the absence of T cell growth factor. Cord blood T cells were rapidly transformed when co-cultivated with irradiated CM cells. Heterologous competition radioimmunoassays using purified HTLV-I p24 showed the expression of HTLV-IIMO-related protein in these cells. Electron microscopy of the CM cells showed the presence of intracellular and extracellular type C viral particles. Comparison of the proviral genome in the CM cell line and the prototype HTLV-IIMO-containing cell line (MO) by molecular hybridization with probes specific for HTLV-IIMO indicated that restriction cleavage sites were identical. The fresh peripheral blood leukocytes of the patient contained two complete copies of the proviral genome, despite the lack of HTLV-II p24 expression. The virus from the cell line CM is designated as HTLV-IICM to distinguish it from the original HTLV-IIMO isolate.
Kalyanaraman
V S
VS
Narayanan
R
R
Feorino
P
P
Ramsey
R B
RB
Palmer
E L
EL
Chorba
T
T
McDougal
S
S
Getchell
J P
JP
Holloway
B
B
Harrison
A K
AK
D000328:Adult; D002460:Cell Line; D002472:Cell Transformation, Viral; D017977:Deltaretrovirus / Q000235:genetics / Q000302:isolation & purification*; D005814:Genes, Viral; D006467:Hemophilia A / Q000382:microbiology*; D006801:Humans; D008297:Male; D010198:Pancytopenia / Q000382:microbiology*; D013601:T-Lymphocytes / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis
D014764:Viral Proteins