A practical cytopathic effect/dye-uptake interferon assay for routine use in the clinical laboratory.
The clinical value of interferon (IFN) level determinations has been demonstrated, but a practical assay procedure for routine use in the diagnostic laboratory has not been available. The authors examined the susceptibility of five cell lines (WISH, HEp-2, Vero, A549, and WI-26VA4) to vesicular stomatitis virus (VSV) and encephalomyocarditis virus (EMCV), and compared the response of these cells to the inhibitory activity of IFN-alpha and IFN-gamma for the respective viral cytopathic effect (CPE). The WISH-EMCV system was the most sensitive for IFN-alpha, and was approximately as sensitive as the HEp-2-VSV system for IFN-gamma. WISH cells were found to be significantly more sensitive for both IFN-alpha and IFN-gamma when EMCV, instead of VSV, was used (P less than 0.001). Therefore, the latter system served as the basis for developing a CPE dye-uptake procedure that was found to be considerably more rapid but slightly less sensitive than the conventional technic. However, both procedures were equally reproducible and should be suitable for automation.
Yousefi
S
S
Escobar
M R
MR
Gouldin
C W
CW
D000650:Amnion; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D019411:Clinical Laboratory Techniques; D004396:Coloring Agents; D003588:Cytopathogenic Effect, Viral; D004686:Encephalomyocarditis virus / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007370:Interferon Type I / Q000032:analysis* / Q000494:pharmacology; D007371:Interferon-gamma / Q000032:analysis* / Q000494:pharmacology; D007822:Laryngeal Neoplasms; D008175:Lung Neoplasms; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014779:Virus Replication
D004396:Coloring Agents; D007370:Interferon Type I; D007371:Interferon-gamma