title: The binding of IgG1 containing immune complexes to the FcR of allogenically activated T cells induces changes in the membrane potential and the cell surface charge.
abstract: The effect on membrane potential and cell surface charge of binding immune complexes containing IgG1 and IgG2a monoclonal antibodies to Fc receptors was studied in resting and allogenically activated murine T cells. IgG1 complexed by antigen or heat aggregation induced electrophysiological changes on activated T cells. A biphasic alteration of membrane potential was detected by measurement of the intra- and extracellular distribution of the fluorescent dye, DiOC6. A short-lived hyperpolarization, detectable for 4-6 min after adding the respective ligand, was followed by a longer lasting depolarization. The cell surface charge, measured by cell electrophoresis, was also changed. This alteration was detected 2-4 hr after addition of immune complexes and disappeared by the 8th hr of incubation. Monoclonal antibody 2.4.G2, reactive with mouse FcR, induced a similar membrane potential response on activated T cells, but did not affect the cell surface charge. Monomeric IgGs and complexes of IgG2a did not modify these parameters. FcR ligands had no effect on the studied characteristics of resting T cells.
authors.affiliation: Institute of Biochemistry, Wilhelm-Pieck-University Rostock, G.D.R.
authors.forename: C
authors.initials: C
authors.lastname: Huckel
authors.forename: M
authors.initials: M
authors.lastname: Sandor
authors.forename: H L
authors.initials: HL
authors.lastname: Jenssen
authors.forename: J
authors.initials: J
authors.lastname: Rychly
authors.forename: J
authors.initials: J
authors.lastname: Brock
authors.forename: J
authors.initials: J
authors.lastname: Gergely
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000378:metabolism*; D002462:Cell Membrane / Q000502:physiology; D004594:Electrophysiology; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D008213:Lymphocyte Activation; D008564:Membrane Potentials; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011961:Receptors, Fc / Q000378:metabolism*; D017452:Receptors, IgG; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*
chemical_list: D000936:Antigen-Antibody Complex; D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG