title: The amino acid sequence of ribonuclease N1, a guanine-specific ribonuclease from the fungus Neurospora crassa.
abstract: The complete amino acid sequence of ribonuclease N1 (RNase N1), a guanine-specific ribonuclease from a fungus, Neurospora crassa, was determined by conventional protein sequencing, using peptide fragments obtained by tryptic digestion of cyanogen bromide-treated RNase N1 and by Staphylococcus aureus V8 protease digestion of heat-denatured RNase N1. The results showed that the protein is composed of a single polypeptide chain of 104 amino acid residues cross-linked by two disulfide bonds and has a molecular weight of 11,174: (sequence; see text) (Disulfide bonds: C2-C10, C6-C103) The amino acid sequence was homologous with those of RNase T1 (65% identity) and related microbial RNases.
authors.affiliation: Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003488:Cyanogen Bromide; D003553:Cystine; D004722:Endoribonucleases / Q000032:analysis*; D008969:Molecular Sequence Data; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification; D006163:Ribonuclease T1 / Q000032:analysis*; D012260:Ribonucleases / Q000032:analysis; D013211:Staphylococcus aureus / Q000201:enzymology; D009626:Terminology as Topic; D014357:Trypsin / Q000378:metabolism
chemical_list: D000596:Amino Acids; D010455:Peptides; D003553:Cystine; D004722:Endoribonucleases; D012260:Ribonucleases; D006163:Ribonuclease T1; D010447:Peptide Hydrolases; D014357:Trypsin; D003488:Cyanogen Bromide