Effect of intratracheal inoculation of Pasteurella haemolytica cytotoxin on the integrity of rat lung.
This study was conducted to investigate the in vivo effect of a single intratracheal inoculation of Pasteurella haemolytica cytotoxin on the rat lung. Changes in the biochemical and cytological composition of bronchoalveolar lavage fluid were used to estimate the magnitude of pulmonary cell injury, inflammatory response, vascular permeability and functional status of pulmonary alveolar macrophages. Effect of treatment was compared with rats intratracheally inoculated with supernatants of Pasteurella multocida or with sterile physiological saline solution (vehicle). Results indicated that Pasteurella haemolytica supernatants were not significantly toxic for the lungs of rats.
Lopez
A
A
Animal Sciences Wing, Alberta Environmental Centre, Vegreville.
Yong
S
S
Shewen
P
P
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis; D002470:Cell Survival; D003603:Cytotoxins / Q000633:toxicity*; D008168:Lung / Q000166:cytology* / Q000502:physiology; D008264:Macrophages / Q000166:cytology* / Q000502:physiology; D010101:Oxygen Consumption; D010325:Pasteurella; D051381:Rats; D011916:Rats, Inbred F344; D013047:Specific Pathogen-Free Organisms; D014132:Trachea
D001427:Bacterial Toxins; D003603:Cytotoxins