Altered form of subunit 6 of mitochondrial ATP synthase complex in oligomycin-resistant mutants of Chinese hamster ovary cells.
Using an antiserum generated against a synthetic peptide predicted from the DNA sequence of the ATPase 6 gene of the mitochondrial DNA, we demonstrate that mitochondria from two oligomycin-resistant Chinese hamster ovary cell lines with a defined mutation in the ATPase 6 gene synthesize an altered ATPase 6 gene product. This altered gene product migrates in sodium dodecyl sulfate-polyacrylamide gels as if it has a molecular mass that is larger by 1000 daltons than the wild-type ATPase 6 gene product. We also demonstrate that mitochondria from four other independently isolated oligomycin-resistant Chinese hamster ovary mutant cell lines contain a similar altered ATPase 6 gene product. These results suggest that all six oligomycin-resistant cell lines have a similar mutation in the ATPase 6 gene of the mitochondrial DNA that encodes subunit 6 of the ATP synthase complex.
Holmans
P L
PL
Biology Program, University of Texas at Dallas, Richardson 75083-0688.
Breen
G A
GA
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D004351:Drug Resistance; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology*; D008970:Molecular Weight; D009154:Mutation; D009840:Oligomycins / Q000494:pharmacology
D007527:Isoenzymes; D009840:Oligomycins; D000251:Adenosine Triphosphatases