title: Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization.
abstract: Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0 degree projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 A. Our structural results are in agreement with the model of Henderson et al. [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Radermacher
authors.forename: M
authors.initials: M
authors.lastname: Kessel
authors.forename: J
authors.initials: J
authors.lastname: Frank
authors.forename: T E
authors.initials: TE
authors.lastname: King
mesh_terms: D019826:Cholic Acid; D002793:Cholic Acids; D003460:Crystallization; D003576:Electron Transport Complex IV; D005583:Fourier Analysis; D008081:Liposomes; D008854:Microscopy, Electron; D008958:Models, Molecular; D012995:Solubility
chemical_list: D002793:Cholic Acids; D008081:Liposomes; D003576:Electron Transport Complex IV; D019826:Cholic Acid