[Postradiation inhibition of poly ADP ribose polymerase is not determined by internucleosomal fragmentation of DNA].
Postirradiation changes in poly(ADP-ribose) polymerase activity in nuclei and permeable thymocytes were studied. The incorporation of 14C-NAD into poly(ADP-ribose) was virtually invariable during the first 60 min after irradiation at a dose of 10 Gy, but after 2 and 3 h it made up, as compared to the control, 60 and 45% for nuclei, and 65 and 55% for permeable thymocytes, respectively. It was shown that the internucleosomal fragmentation of DNA was not responsible for the observed changes in poly(ADP-ribose) polymerase activity.
Nelipovich
P A
PA
Nikonova
L V
LV
Umanskiĭ
S R
SR
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002588:Cesium Radioisotopes; D004247:DNA / Q000528:radiation effects*; D005720:Gamma Rays; D008297:Male; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D009707:Nucleosomes / Q000528:radiation effects*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011833:Radiation Injuries, Experimental / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000201:enzymology
D002588:Cesium Radioisotopes; D009707:Nucleosomes; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D009244:NAD+ Nucleosidase
Postradiatsionnoe ingibirovanie poli(ADF-ribozo)-polimerazy ne opredeliaetsia mezhnukleosomnoĭ fragmentatsieĭ DNK.