Increased superoxide radical production evokes inducible DNA repair in Escherichia coli.
Paraquat induced the SOS response in Escherichia coli. This was measured in terms of acquired resistance towards UV lethality in a wild-type strain and in terms of appearance of beta-galactosidase activity in a din::Mu d(Ap lac) fusion strain. However measured, the induction of the SOS response by paraquat was entirely dioxygen-dependent; whereas induction of the SOS response by mitomycin C was independent of the presence of dioxygen. As expected, recA(Def) and lexA(Ind-) isogenic strains did not show the SOS response. It appears likely that O-2, whose intracellular production is increased by paraquat, leads to DNA damage which in turn induces the SOS response.
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D004260:DNA Repair / Q000187:drug effects; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism; D010269:Paraquat / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000096:biosynthesis
D013481:Superoxides; D001616:beta-Galactosidase; D010269:Paraquat; D010100:Oxygen