5-Nucleotidase activity in isolated follicular dendritic cells.
Follicular dendritic cells isolated from mouse lymph nodes were incubated in the presence of AMP to test 5-nucleotidase (5-Nase) activity. Ultrastructural observations showed the presence of 5-Nase on external membranes but also some activity inside the nucleus. 5-Nase was found associated to Fc receptors labelled with homologous immunoglobulins fixed on colloidal gold particles. Lymphocytes and macrophages, found in association with the follicular dendritic cells, were either 5-Nase positive or negative. The hypothetical roles played by 5-Nase in germinal centers are discussed.
Heinen
E
E
Kinet-Denoël
C
C
Simar
L J
LJ
D015720:5'-Nucleotidase; D000818:Animals; D006651:Histocytochemistry; D008198:Lymph Nodes / Q000201:enzymology* / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D009708:Nucleotidases / Q000378:metabolism*; D011961:Receptors, Fc / Q000378:metabolism
D011961:Receptors, Fc; D009708:Nucleotidases; D015720:5'-Nucleotidase