Recombinant human interferon-gamma induces increased IgE receptor expression on human platelets.
Human recombinant interferon-gamma (IFN-gamma) significantly increased the expression of receptors for IgE (Fc epsilon RII) on blood platelets. Fc epsilon RII was measured by specific binding of 125I-labeled IgE or flow cytometry experiments. Scatchard analysis of 125I-labeled IgE binding curves revealed that treatment with IFN-gamma increased the number of Fc epsilon RII but did not change the value of the association constant of Fc epsilon RII for 125I-labeled IgE. IFN-alpha had no effect on the expression or affinity of Fc epsilon RII. In addition to Fc epsilon RII, IFN-gamma also modified the expression of the glycoprotein IIb-IIIa complex on the platelet membrane.
Pancré
V
V
Centre d'Immunologie et de Biologie Parasitaire, Institut Pasteur, Lille, France.
Joseph
M
M
Capron
A
A
Wietzerbin
J
J
Kusnierz
J P
JP
Vorng
H
H
Auriault
C
C
D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis; D011961:Receptors, Fc / Q000096:biosynthesis*; D017455:Receptors, IgE; D011994:Recombinant Proteins / Q000494:pharmacology; D013268:Stimulation, Chemical
D010980:Platelet Membrane Glycoproteins; D011961:Receptors, Fc; D017455:Receptors, IgE; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D007371:Interferon-gamma