In vitro immunological function of human corneal fibroblasts.
Gamma-interferon-induced HLA-DR-like antigens on normal human fibroblasts were compared functionally to HLA-DR antigens on peripheral blood mononuclear leucocytes (PBML) from the same individual. Irradiated DR+ fibroblasts did not stimulate allogeneic PBML to divide in a mixed lymphocyte fibroblast culture (MLFC) as determined by 3H-thymidine incorporation, although lymphocytes autologous to the fibroblasts stimulated normally. Co-culture with interleukin 1 (IL-1), IL-2, 2-mercaptoethanol and indomethacin had no effect on MLFC. Pretreatment of stimulator fibroblasts with neuraminidase to modify sialoglycoproteins increased responses only modestly. Both DR+ and DR- fibroblasts inhibited a normal mixed lymphocyte culture (MLC) response in a dose-dependent fashion. These results suggest that the induction of HLA-DR-like molecules on fibroblasts is not sufficient to initiate host sensitization and that fibroblasts may inhibit normal lymphoproliferation.
Young
E
E
Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Stark
W J
WJ
D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology / Q000276:immunology*; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008623:Mercaptoethanol / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D012794:Sialic Acids / Q000494:pharmacology
D007375:Interleukin-1; D007376:Interleukin-2; D012794:Sialic Acids; D008623:Mercaptoethanol; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D007213:Indomethacin