Transcription of the Bacillus subtilis bacteriophage phi 3T in vitro.
The in vitro transcription pattern of Bg/II-digested phi 3T DNA is described. Eight Bg/II fragments that hybridized to in vitro transcription products were unequivocally identified. A further hybridizing region corresponding to a Bg/II triplet was also revealed, giving a total of nine to 11 Bg/II fragments. These represent 47 to 53% of the phi 3T genome. Transcription was shown to initiate within Bg/II fragments B, G, C, H, I, F and J, implying that all of these contain at least one promoter. The relevance of these data to the construction of a cloning vector based on phi 3T is discussed.
Kenny
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Atkinson
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Hartley
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D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D002474:Cell-Free System; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000201:enzymology; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D014158:Transcription, Genetic
D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C018492:GCCNNNNNGGC-specific type II deoxyribonucleases