Influence of dietary lipids on microsomal membranes from chick breast muscle.
1. The effect of different dietary fat intake on the lipid composition and fluidity of microsomal membranes as well as in the enzymatic activity of the Ca2+-ATPase from chick breast muscle was investigated. 2. When a standard diet was supplemented with 10% sunflower seed oil, an increase in the relative amounts of unsaturated fatty acids and membrane fluidity and a decrease in the cholesterol content was observed. 3. The presence of 6% cholesterol in the diet does not modify the fatty acid composition and the fluidity of the membrane but increased, in a low extension, the cholesterol content. 4. The provision of the sunflower seed oil-rich diet supplemented with cholesterol just 48 hr before death promoted an increase in the relative amounts of unsaturated fatty acids and cholesterol content whereas the membrane fluidity decreased in a significant extent. 5. Despite that dietary lipids gave rise in some cases to changes in lipid composition and in the physical state of the microsomal membrane, neither the Ca2+ uptake capacity nor the ATPase activity were significantly affected.
Soler
F
F
Departamento de Bioquimica y Biologia Molecular, Universidad de Murcia, Spain.
Alejandre
M J
MJ
Garcia-Gonzalez
M
M
Segovia-Parra
J L
JL
Fernandez-Belda
F
F
Gomez-Fernandez
J C
JC
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002645:Chickens; D002791:Cholesterol, Dietary / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*
D002791:Cholesterol, Dietary; D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D000252:Calcium-Transporting ATPases