title: Molecular weight of human brain neutral sphingomyelinase determined in situ by the radiation inactivation method.
abstract: The radiation inactivation method was used to determine the molecular weight of membrane-bound neutral sphingomyelinase from normal human brain. Inactivation curves showed a molecular mass of 167,000 +/- 32,000. Molecular weights of two control enzymes, beta-N-acetylglucosaminidase and nonspecific beta-glucosidase, determined by the same procedure, were consistent with previous reports.
authors.forename: T
authors.initials: T
authors.lastname: Levade
authors.forename: M
authors.initials: M
authors.lastname: Potier
authors.forename: R
authors.initials: R
authors.lastname: Salvayre
authors.forename: L
authors.initials: L
authors.lastname: Douste-Blazy
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008970:Molecular Weight; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism* / Q000528:radiation effects; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase; D001617:beta-Glucosidase; D000118:Acetylglucosaminidase