title: [Participation of coronaviruses in the etiology of respiratory diseases in calves].
abstract: Studied was the Bovine corona virus as taking part in the epizootiology of respiratory diseases in calves. On 4 out of the 7 investigated farms there was a rise of the antibodies against the virus. Two strains were isolated from 2 diseased calves, using tissue culture of trachea. They were the III 213/81 and III 214/81 strains and were identified as bovine corona viruses. Test calves experimentally infected via the trachea with the III 214/81 strain responded with a slight and transient rise of body temperature and a slight cough. The virus was reisolated from the nasal discharge of 3 diseased calves as well as from the feces of one affected calf. The direct immunofluorescence method was employed to establish cytoplasmic fluorescence between the 4th and the 8 h day of infection. The immune response observed with the infected animals confirmed the development of the corona-virus infection. The clinical observations with calves on both the farms with spontaneous infections and those with experimentally induced infections showed that the bovine corona virus could not cause a strongly manifested respiratory disease.
authors.forename: I
authors.initials: I
authors.lastname: Sizov
authors.forename: B
authors.initials: B
authors.lastname: Mitov
authors.forename: Kh E
authors.initials: KhE
authors.lastname: Kharalambiev
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000209:etiology*; D003332:Coronaviridae / Q000276:immunology; D003333:Coronaviridae Infections / Q000175:diagnosis / Q000209:etiology / Q000662:veterinary*; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000209:etiology / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral
vernacular_title: Uchastie na koronavirusi v etiologiiata na respiratornite zaboliavaniia po teletata.