Subclass specificity of the Fc receptor for human IgG on K562.
The erythroleukemic cell line K562 bears a 40-kDa Fc receptor (Fc gamma RII) serologically related to and with a similar molecular weight as the Fc gamma R present on a broad range of leukocytes. The human IgG subclass specificity of the Fc gamma R on K562 was investigated using IgG aggregates of defined size, obtained from purified human myeloma proteins. The monoclonal antibody IV.3, which reacts with the Fc gamma RII present on various cell types, totally prevented binding of 125I-IgG2 trimers to K562. Experiments with radiolabeled IgG2 trimers showed that K562 cells bound a mean of 156,764 +/- 9895 molecules per cell with an association constant (Ka) of 1.8 +/- 0.7 X 10(8) M-1. Similar results were obtained with IgG3 oligomers. IgG3 and IgG2 trimers were about two- to threefold more effective in inhibiting binding of 125I-IgG2 trimers to K562 than IgG1 and IgG4 trimers. These results were confirmed by inhibition experiments using IgG monomers. The subclass specificity of the Fc gamma RII on K562 (i.e., IgG2 = IgG3 greater than IgG1 = IgG4) is quite distinct from the one reported for the Fc gamma RI and III of human cells (i.e., IgG1 = IgG3 greater than IgG4 and IgG2).
Chiofalo
M S
MS
Department of Pathology, Medical University of South Carolina, Charleston 29425.
Teti
G
G
Goust
J M
JM
Trifiletti
R
R
La Via
M F
MF
D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology / Q000378:metabolism*; D046911:Macromolecular Substances; D011869:Radioligand Assay; D011961:Receptors, Fc / Q000145:classification* / Q000276:immunology / Q000502:physiology; D017452:Receptors, IgG
D007074:Immunoglobulin G; D046911:Macromolecular Substances; D011961:Receptors, Fc; D017452:Receptors, IgG