Possible involvement of Ly-1 B cells in the effector phase of IgG suppression mediated by suppressor T cell factor.
The effector phase of IgG suppression mediated by an antigen-specific suppressor T cell factor (TsF) was studied. The monoclonal TsF of an inducer type derived from a KLH-specific suppressor T cell hybridoma (34S-704) suppressed IgG response mounted by DNP-primed B cells (anti-Thy-1 treated spleen cells) and KLH-specific cloned helper T cells only in the presence of unprimed Thy-1-, Ly-1+, I-J+, Ig+ cells. The addition of naive Ly-1+ cells to the culture of DNP-primed Ly-1/Thy-1 depleted B cells and KLH-Th clones augments anti-DNP IgG responses, and KLH-TsF suppressed the enhanced parts of IgG responses. The Ly-1+ cell seems to be a target of TsF, because naive spleen cells treated either with anti-Ly-1 or with anti-MIg failed to absorb TsF, whereas TsF activity was absorbed with whole spleen cells and anti-Thy-1 treated spleen cells. The functional role of Ly-1 B cells and possible mechanisms in the effector phase of TsF-mediated IgG suppression will be discussed.
Ono
K
K
Department of Immunology, School of Medicine, Chiba University, Japan.
Kato
F
F
Taniguchi
M
M
D000818:Animals; D000950:Antigens, Ly; D001402:B-Lymphocytes / Q000276:immunology*; D000949:Histocompatibility Antigens Class II; D007108:Immune Tolerance; D007074:Immunoglobulin G / Q000096:biosynthesis*; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000950:Antigens, Ly; D000949:Histocompatibility Antigens Class II; C043911:I-J-antigen; D007074:Immunoglobulin G; D013491:Suppressor Factors, Immunologic