title: Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of F1 subunit.
abstract: The amino acid sequence of respiratory syncytial virus fusion protein (Fo) was deduced from the sequence of a partial cDNA clone of mRNA and from the 5' mRNA sequence obtained by primer extension and dideoxysequencing. The encoded protein of 574 amino acids is extremely hydrophobic and has a molecular weight of 63371 daltons. The site of proteolytic cleavage within this protein was accurately mapped by determining a partial amino acid sequence of the N-terminus of the larger subunit (F1) purified by radioimmunoprecipitation using monoclonal antibodies. Alignment of the N-terminus of the F1 subunit within the deduced amino acid sequence of Fo permitted us to identify a sequence of lys-lys-arg-lys-arg-arg at the C-terminus of the smaller N-terminal F2 subunit that appears to represent the cleavage/activation domain. Five potential sites of glycosylation, four within the F2 subunit, were also identified. Three extremely hydrophobic domains are present in the protein; a) the N-terminal signal sequence, b) the N-terminus of the F1 subunit that is analogous to the N-terminus of the paramyxovirus F1 subunit and the HA2 subunit of influenza virus hemagglutinin, and c) the putative membrane anchorage domain near the C-terminus of F1.
authors.forename: N
authors.initials: N
authors.lastname: Elango
authors.forename: M
authors.initials: M
authors.lastname: Satake
authors.forename: J E
authors.initials: JE
authors.lastname: Coligan
authors.forename: E
authors.initials: E
authors.lastname: Norrby
authors.forename: E
authors.initials: E
authors.lastname: Camargo
authors.forename: S
authors.initials: S
authors.lastname: Venkatesan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*; D014760:Viral Fusion Proteins
chemical_list: D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D004262:DNA Restriction Enzymes