Effects of fructose 2,6-bisphosphate and glucose 1,6-bisphosphate on phosphofructokinase from chicken erythrocytes.
1. Phosphofructokinase (EC 2.7.1.11) from chicken erythrocytes is activated by fructose 2,6-bisphosphate, glucose 1,6-bisphosphate and AMP, and it is inhibited by 2,3-bisphosphoglycerate and inositol hexaphosphate. 2. The stimulatory effects produced by the two bisphosphorylated hexoses are additive and the effects produced by fructose 2,6-bisphosphate and by AMP are synergistic. 3. The activatory effect produced by fructose 2,6-bisphosphate is counteracted by fructose 1,6-bisphosphate. 4. The inhibition produced by both 2,3-bisphosphoglycerate and inositol hexaphosphate is released by fructose 2,6-bisphosphate. 5. It is concluded that, like phosphofructokinase from mammalian tissues, the enzyme from chicken erythrocytes can be modulated by the relative concentrations of those metabolites.
Espinet
C
C
Facultat de Medicina, Universitat de Barcelona, Department de Ciències Fisiològiques Humanes i de la Nutrició, Spain.
Bartrons
R
R
Carreras
J
J
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D004912:Erythrocytes / Q000201:enzymology*; D005635:Fructosediphosphates / Q000494:pharmacology*; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000494:pharmacology*; D006598:Hexosediphosphates / Q000494:pharmacology*; D007700:Kinetics; D010732:Phosphofructokinase-1 / Q000097:blood*
D005635:Fructosediphosphates; D005958:Glucosephosphates; D006598:Hexosediphosphates; D000249:Adenosine Monophosphate; D019298:Glucose-6-Phosphate; C027652:fructose 2,6-diphosphate; C016473:glucose-1,6-bisphosphate; D010732:Phosphofructokinase-1