title: Deletion of the gag region from FBR murine osteosarcoma virus does not affect its enhanced transforming activity.
abstract: Both FBJ murine osteosarcoma virus (FBJ-MSV) and FBR-MSV induce transformation in tissue culture and osteogenic sarcomas in mice. In tissue culture, however, FBR-MSV induces larger foci with a shorter latency than those induced by FBJ-MSV. Transformation is dependent on expression of the fos oncogene in FBJ-MSV and of a gag-fos fusion protein in FBR-MSV. We have determined that the gag sequences can be deleted from FBR-MSV without affecting the high transforming activity of this virus in comparison to FBJ-MSV. The resultant virus, designated FBJ/R-MSV, has a protein coding region that is half the size of that of FBR-MSV and about one-third smaller than that of FBJ-MSV. Thus, FBJ/R-MSV will provide a useful tool for studying the transforming activity of the fos oncogene.
authors.forename: A D
authors.initials: AD
authors.lastname: Miller
authors.forename: I M
authors.initials: IM
authors.lastname: Verma
authors.forename: T
authors.initials: T
authors.lastname: Curran
mesh_terms: D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D015683:Gene Products, gag; D005814:Genes, Viral; D051379:Mice; D009857:Oncogenes; D012516:Osteosarcoma / Q000382:microbiology; D051381:Rats; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D015683:Gene Products, gag; D014764:Viral Proteins