Antibody development and cellular immune responses in sheep immunized and challenged with Sarcocystis tenella sporocysts.
Four specific-pathogen-free (SPF) sheep were experimentally infected with 10(3) or 10(4) Sarcocystis tenella (syn. S. ovicanis) sporocysts and another two sheep served as uninfected controls. All sheep were challenged 49 days later by infection with 2.5 X 10(5) sporocysts and their humoral and cellular responses to infection and challenge were assessed weekly by enzyme immunoassays and lymphocyte transformation assays. The control sheep died from acute sarcocystosis 29-30 days after challenge, whereas the immunized sheep survived and were protected against acute disease. Specific IgM and IgG antibodies were detected in the immunized sheep from 28 days after infection onwards. Lymphocytes collected before and after challenge did not exhibit any significant differences in their responses to stimulation with S. tenella cystozoite or sporozoite antigens. Furthermore, lymphocytes collected before challenge did not differ from the controls in their responses to stimulation with the mitogens lipopolysaccharide or phytohaemagglutinin. However, lymphocytes collected after challenge did exhibit increased blastogenic responses to stimulation with both mitogens from 21-28 days after challenge onwards. The infected sheep were necropsied 46 days after challenge, and histological and ultrastructural studies revealed numerous infiltrates of lymphocytes, histiocytes and plasma cells in the skeletal muscles, sometimes in association with degenerating parasitic cysts and macrophage myophagia. Parasites were not completely eliminated nor prevented from further establishment, therefore the protective immunity was not sterile but rather a state of premunition.
O'Donoghue
P J
PJ
Central Veterinary Laboratories, Department of Agriculture, Adelaide, South Australia.
Wilkinson
R G
RG
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D007111:Immunity, Cellular; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D009132:Muscles / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D012522:Sarcocystis / Q000276:immunology* / Q000648:ultrastructure; D012523:Sarcocystosis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000473:pathology; D013047:Specific Pathogen-Free Organisms
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M