H-2Ld antigen encoded by a recombinant retrovirus genome is expressed on the surface of infected cells.
A recombinant murine retrovirus was constructed which contains, within its genome, a truncated version of the gene encoding the murine H-2Ld major histocompatibility antigen. The H-2Ld gene, which was inserted 3' of the env splice acceptor site in the recombinant retrovirus MSV-neo, lacked the 5' promoter and TATA sequences and the 3' transcription termination and polyadenylate addition sites of the normal H-2Ld gene. Transfection of the MSV-neo/H-2Ld plasmid (pLTV-11) into Y-2 cells resulted in the production of the transmissible recombinant retrovirus LTV-11. Cells infected with LTV-11 virus were resistant to the eucaryotic antibiotic G418 and expressed H-2Ld on the cell surface. These infected cells contained a viral RNA species which possessed both the H-2Ld and the neomycin resistance gene sequences but did not contain significant levels of the smaller H-2Ld-specific mRNA. The H-2Ld antigen expressed on the surface of infected cells functioned as a target for cytolytic T cells specific for the H-2Ld antigen.
Weis
J H
JH
Reiss
C S
CS
Mulligan
R C
RC
Burakoff
S J
SJ
Seidman
J G
JG
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002460:Cell Line; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D005822:Genetic Vectors; D006183:H-2 Antigens / Q000032:analysis / Q000235:genetics*; D064532:Histocompatibility Antigen H-2D; D006825:Hybridomas / Q000276:immunology; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
D000954:Antigens, Surface; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D