title: Interferon gamma production by herpes simplex virus antigen-specific T cell clones from patients with recurrent herpes labialis.
abstract: Nineteen herpes simplex virus (HSV) antigen-specific human T lymphocyte clones were established from three volunteers with recent recurrent herpes labialis. All produced interferon gamma (IFN-gamma) at titres of 200 to 700 units/ml when cultured in vitro with HSV antigen and irradiated peripheral blood mononuclear cells (PBMC) as filler cells. All 10 of those clones whose phenotype was determined were Leu 4+, Leu 2-, Leu 3+. Interleukin 2 alone failed to induce IFN-gamma in titres greater than 10 units/ml from these clones cultured at 10(4)/0.2 ml/well. However, the effect of different accessory or filler cells on IFN-gamma production by clones was quite marked. For example, high titres were produced when irradiated PBMC or plastic-adherent cells (predominantly monocytes) were added and low titres when macrophages and irradiated Epstein-Barr virus-transformed B (EBV-B) cells were added. When tested for HSV antigen-stimulated IFN production alone, the irradiated PBMC and adherent cells produced low titres, but no detectable interferon was produced by the others. However, with higher concentrations of EBV-B cells, low concentrations of IFN-alpha were occasionally produced. Irradiation strikingly reduced IFN-alpha-production by PBMC. The IFN-alpha and -gamma produced by accessory cells may contribute to total IFN production by priming the production by cloned cells, and acting in synergy with IFN-gamma produced by the cloned cells. Alternatively, the effect may be due to the presence of permissive concentrations of other lymphokines such as the interleukins. Interferon production by cloned T lymphocytes in the presence of non-producing macrophages was maximal within 24 h, much faster than with a similar polyclonal system, although attaining lower titres. EBV-B cells from only one of three patients supported antigen-specific lymphocyte activation. Almost all cells of the three cell lines expressed DR antigens, while DS/DC antigens were also expressed on nearly all cells of the antigen-presenting line and, at lower densities, on two-thirds of the cells of the other two lines.
authors.forename: A L
authors.initials: AL
authors.lastname: Cunningham
authors.forename: P A
authors.initials: PA
authors.lastname: Nelson
authors.forename: C G
authors.initials: CG
authors.lastname: Fathman
authors.forename: T C
authors.initials: TC
authors.lastname: Merigan
mesh_terms: D000938:Antigen-Presenting Cells / Q000378:metabolism*; D000956:Antigens, Viral / Q000276:immunology; D002999:Clone Cells / Q000187:drug effects / Q000378:metabolism; D006680:HLA Antigens / Q000032:analysis; D006560:Herpes Labialis / Q000378:metabolism*; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000494:pharmacology; D012008:Recurrence; D018139:Simplexvirus / Q000276:immunology; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000956:Antigens, Viral; D006680:HLA Antigens; D007370:Interferon Type I; D007376:Interleukin-2; D007371:Interferon-gamma