Dihydrocytochalasin B promotes adipose conversion of 3T3 cells.
Differentiation of preadipose 3T3-F442A cells into adipose cells is accelerated by the addition of dihydrocytochalasin B. The effect of the drug on 3T3-C2 cells is more marked: these cells are practically unable to differentiate in the absence of H2CB but a long-term exposure to the drug enables the cells to accumulate lipid droplets in medium supplemented with fetal calf serum and insulin. During their differentiation under these conditions the 3T3-C2 cells develop markers typical of adipose cells: glycerophosphate dehydrogenase, ATP-citrate lyase, fatty acid synthetase and glycerophosphate acyltransferase.
Pairault
J
J
Institut National de la Santé et de la Recherche Médicale, Unité 282, Hôpital Henri-Mondor, Créteil, France.
Lasnier
F
F
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D002999:Clone Cells; D003571:Cytochalasin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism; D051379:Mice
D004798:Enzymes; C024794:dihydrocytochalasin B; D003571:Cytochalasin B