[Detection of macrophage activity in the blood and synovia of chronic polyarthritis patients using an activated oxygen microassay].
A microassay was developed for the estimation of macrophage (M phi)-activation by released activated oxygen. From peripheral blood or synovia M phi are isolated. O-2 which is released by activated M phi is photometrically detected by cytochrome c reduction. Differences in O-2-production do not exist between monocytes of rheumatoid arthritis patients and controls. In contrast synovial M phi from rheumatoid arthritis patients show increased levels of O-2-production.
Schmitz
E
E
Warnatz
H
H
D001172:Arthritis, Rheumatoid / Q000276:immunology*; D003574:Cytochrome c Group / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D010100:Oxygen / Q000378:metabolism*; D013582:Synovial Fluid / Q000276:immunology*
D003574:Cytochrome c Group; D010100:Oxygen
Nachweis der Makrophagenaktivität in Blut und Synovia von chronisch Polyarthritis-Kranken mittels eines aktivierten Sauerstoff-Mikroassays.