Smart2, a cosmid vector with a phage lambda origin for both systematic chromosome walking and P-element-mediated gene transfer in Drosophila.
We describe a new phage-lambda-replicon-based cosmid vector suitable for both chromosome walking and P-element-mediated transformation in Drosophila. Its unique BamHI cloning site is flanked by the promoters for the SP6 and T7-encoded RNA polymerases, permitting the synthesis of probes complementary to the ends of the cloned inserts for library screening. The selectable marker is tet for bacterial cell transformation and neo for Drosophila transformation expressed under the control of the Drosophila hsp70 promoter.
Speek
M
M
Department of Biochemistry, Imperial College, London, U.K.
Raff
J W
JW
Harrison-Lavoie
K
K
Little
P F
PF
Glover
D M
DM
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D003360:Cosmids; D004330:Drosophila / Q000235:genetics*; D005822:Genetic Vectors; D006360:Heat-Shock Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D012093:Replicon; D014162:Transfection
D006360:Heat-Shock Proteins