IL-2 production in the autologous mixed lymphocyte reaction of patients with juvenile rheumatoid arthritis.
Interleukin 2 (IL-2) production in the CD4/non-T cell autologous mixed lymphocyte reaction (AMLR) was estimated for patients with juvenile rheumatoid arthritis (JRA). A cellular IL-2 assay (CILA) consisting of co-cultures of indicator cells, CTLL-2, added to CD4/non-T cell AMLR cultures was used. As both CD4 and CTLL-2 cell proliferation are IL-2 dependent, results of the CILA assay were taken as a reflection of the total IL-2 in the co-culture. By this assay most patients with JRA had normal IL-2 production. However, five patients with polyarticular disease had both a low AMLR and low levels of IL-2 production. Three of those tested showed little improvement in the AMLR on addition of recombinant IL-2, suggesting decreased responsiveness to IL-2 as well.
Oen
K
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Department of Pediatrics, University of Manitoba, Winnipeg, Saskatchewan.
Warrington
R
R
Rosenberg
A M
AM
Krzekotowska
D
D
D000293:Adolescent; D001171:Arthritis, Juvenile / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D007959:Lymphocyte Culture Test, Mixed; D008938:Mitosis; D013601:T-Lymphocytes / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D013997:Time Factors
D007376:Interleukin-2