Improved localization of intracellular sites of phosphatases using cerium and cell permeabilization.
A simple permeabilization method has been developed that allows for intracellular localization of acid phosphatase in neutrophils and several types of tissue culture cells with cerium. This permeabilization procedure also facilitates intracellular alkaline phosphatase localization in neutrophils without the loss of cell surface reaction in this cell type. Only the cell surface reaction was detected in the absence of permeabilization. Glutaraldehyde-fixed cells were permeabilized with detergent during the cytochemical reaction. Triton X-100 at 0.0001-0.0002% gave the best results for the enzymes and cell types tested.
Robinson
J M
JM
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001854:Bone Marrow Cells; D002417:Cattle; D002463:Cell Membrane Permeability; D002563:Cerium; D006224:Cricetinae; D006168:Guinea Pigs; D051379:Mice; D008854:Microscopy, Electron; D009504:Neutrophils / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D002563:Cerium; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases