Isolation and characterization of the pMI10 plasmid from Bacillus subtilis, an alpha-amylase producer.
The plasmid DNA pMI10 (5310 bp) was isolated from the alpha-amylase producing strain B. subtilis A18. Thirteen restriction endonucleases were used to digest pMI10 DNA and the restriction map of pMI10 DNA was constructed by mapping PstI (1), HindII (2), BglI (2), BspRI (3) and HindIII (3) sites.
Tichý
P
P
Hartmann
M
M
Holubová
I
I
Krumphanzl
V
V
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D010957:Plasmids; D000516:alpha-Amylases / Q000235:genetics*
D004262:DNA Restriction Enzymes; D000516:alpha-Amylases