Host species specificity of polyomavirus DNA replication is not altered by simian virus 40 72-base-pair repeats.
The simian virus 40 72-base-pair repeats substituted for the polyomavirus enhancer, allowing replication and transcription in mouse 3T6 but not monkey CV-1 cells. A polyomavirus genome containing the entire simian virus 40 control region replicated at low levels in 3T6 and CV-1 cells; however, transcripts were detected only in 3T6 cells. Our results suggest that the simian virus 40 72-base-pair repeats are unable to alter the host species specificity of the complete polyomavirus genome.
Campbell
B A
BA
Villarreal
L P
LP
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004261:DNA Replication; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D005814:Genes, Viral; D051379:Mice; D011120:Polyomavirus / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics*; D013045:Species Specificity; D014158:Transcription, Genetic; D014779:Virus Replication