title: The internal dynamics of gene 32 protein-DNA complexes studied by quasi-elastic light scattering.
abstract: The hydrodynamic properties of large homodisperse single stranded DNAs complexed with the helix destabilizing protein of phage T4, the product of gene 32 (GP32), have been measured. The results suggest a size of the binding site between 8 and 10 nucleotides/GP32 molecule, in reasonable agreement with earlier work on a complex between GP32 and single stranded 145 base DNA. From static light scattering experiments it is concluded that the persistence length of these complexes is about 30 nm, distinctly smaller than the generally accepted value for double stranded DNA. The quasi-elastic light scattering properties of the DNA-GP32 complexes were determined. The variation of the apparent translation diffusion coefficient Dapp with the scattering vector q was analyzed using the discrete ISMF and Rouse-Zimm models [S.C. Lin et al., Biopolymers 17 (1978) 425]. The model parameters that followed from the fit of Dapp versus q2 and from an extensive global analysis of the actually measured autocorrelation functions agreed with the notion that these DNA-protein complexes are indeed rather flexible. The continuous Soda model [K. Soda, Macromolecules 17 (1984) 2365] could successfully explain the variation of Dapp versus q2, assuming a persistence length of 30 nm and a base-base distance in the complex of 0.44 nm.
authors.affiliation: Department of Biophysics, Free University, Amsterdam, The Netherlands.
authors.forename: M E
authors.initials: ME
authors.lastname: Kuil
authors.forename: F
authors.initials: F
authors.lastname: van Mourik
authors.forename: W
authors.initials: W
authors.lastname: Burger
authors.forename: R
authors.initials: R
authors.lastname: van Grondelle
mesh_terms: D010582:Bacteriophage lambda / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004548:Elasticity; D004926:Escherichia coli / Q000378:metabolism; D008027:Light; D008433:Mathematics; D008962:Models, Theoretical; D010957:Plasmids; D012542:Scattering, Radiation; D013604:T-Phages / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D014764:Viral Proteins