Adult rat heart cell culture. The morphological study of tissue fragments on various environments in culture.
This paper reports a modification of Jacobson's method for culturing adult rat heart cells after mechanical and enzyme treatment. For heart cell dissociation, it is useful to wash tissue fragments 3 times with saline A solution after enzyme treatment, and it is important to change the medium immediately after culturing heart cells in order to prevent myocardial cells from degeneration caused by the enzyme solution and toxins in the cell debris.
Yamana
K
K
Kinoshita
T
T
Nakano
R
R
Tokunaga
O
O
Morimatsu
M
M
Nakashima
T
T
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D003599:Cytoskeleton / Q000648:ultrastructure; D008297:Male; D003012:Microbial Collagenase; D008929:Mitochondria, Heart / Q000648:ultrastructure; D009206:Myocardium / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D014357:Trypsin
D003470:Culture Media; D014357:Trypsin; D003012:Microbial Collagenase