title: Monoclonal antibodies NORM-1 and NORM-2 induce more normal behavior of tumor cells in vitro and reduce tumor growth in vivo.
abstract: In this study, large numbers of hybridomas (produced by syngeneic immunization with B16 mouse melanoma and fusion with NS-1 myeloma cells) were screened for the production of antibodies that affected morphology and growth of animal and human tumor cells in vitro. Two such antibodies, NORM-1 and NORM-2 (both IgG2a), inhibited the growth of B16 melanoma cells in soft agar and increased the serum requirements of tumor cells in tissue culture. Antibody NORM-2 also inhibited the growth of SV40-transformed 3T3 cells in agar and caused them to deposit more fibronectin into extracellular matrix. These antibodies thus seem to induce a more normal behavior of tumor cells in vitro. In vivo both antibodies reduced the number of growing lung tumors of B16 melanoma in C57BL/6 mice by 70%-90% when injected 3 days after the tumor cells. By immunoprecipitation of 35S-methionine-labeled cell extracts, NORM-2 antibody recognized a 59 kd protein in B16 mouse and in A375 human melanoma cells but not in 3T3 fibroblasts.
authors.forename: H P
authors.initials: HP
authors.lastname: Vollmers
authors.forename: B A
authors.initials: BA
authors.lastname: Imhof
authors.forename: I
authors.initials: I
authors.lastname: Wieland
authors.forename: A
authors.initials: A
authors.lastname: Hiesel
authors.forename: W
authors.initials: W
authors.lastname: Birchmeier
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D002277:Carcinoma / Q000473:pathology; D002448:Cell Adhesion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007163:Immunosorbent Techniques; D008175:Lung Neoplasms / Q000556:secondary; D008545:Melanoma / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000473:pathology*; D013539:Simian virus 40; D013724:Teratoma / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins