Correlation between PLD repair capacity and the survival curve of human fibroblasts in exponential growth phase: analysis in terms of several parameters.
Published data on the in vitro radiosensitivity of 46 nontransformed fibroblasts of different genetic origins studied in plateau phase with immediate or delayed plating were used to investigate to what extent potentially lethal damage repair capacity is related to intrinsic radiosensitivity (i.e., irradiated in exponential growth phase). While most of the survival curve analysis is conducted in terms of D0, Dq, and the mean inactivation dose D, some of the data are also discussed in terms of the linear-quadratic model parameter alpha. Using D it is shown that: (i) the radiosensitivity of human fibroblasts in exponential growth phase does not significantly differ from that of plateau-phase fibroblasts with immediate plating; (ii) the radiosensitivity of plateau-phase cells with delayed plating is correlated to the radiosensitivity of cells with immediate plating: the more radioresistant the cell strain in exponential growth phase, the higher its repair capacity; (iii) the repair capacity of the cell strains is related to their genetic origin. In conclusion, we suggest that the survival curve of growing cells depends on the repair capacity of the cells.
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Méthodologie en Informatique et Statistique en Médecine (Unité Inserm 194), Paris, France.
Deschavanne
P J
PJ
Debieu
D
D
Malaise
E P
EP
D001260:Ataxia Telangiectasia / Q000235:genetics; D001478:Basal Cell Nevus Syndrome / Q000235:genetics; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects*; D003057:Cockayne Syndrome / Q000235:genetics; D004260:DNA Repair; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D006816:Huntington Disease / Q000235:genetics; D011831:Radiation Genetics; D011836:Radiation Tolerance; D012175:Retinoblastoma / Q000235:genetics