The effect of fructose 2,6-bisphosphate on muscle fructose-1,6-bisphosphatase activity.
Rat and rabbit muscle fructose 1,6-bisphosphatase (D-fructose-1,6-bisphosphate 1-phosphohydrolase, EC 3.1.3.11) are inhibited by fructose 2,6-bisphosphate. In contrast with the liver isozyme, the inhibition of muscle fructose-1,6-bisphosphatase by fructose 2,6-bisphosphate is not synergistic with that of AMP. Activation of fructose-1,6-bisphosphatase by fructose 2,6-bisphosphate has been observed at high concentrations of substrate. An attempt is made to correlate changes in concentrations of hexose monophosphate, fructose 1,6-bisphosphate and fructose 2,6-bisphosphate with changes in fluxes through 6-phosphofructokinase and fructose-1,6-bisphosphatase in isolated epitrochlearis muscle challenged with insulin and adrenaline.
Bosca
L
L
Challiss
R A
RA
Newsholme
E A
EA
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors*; D005635:Fructosediphosphates / Q000378:metabolism / Q000494:pharmacology*; D006598:Hexosediphosphates / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology*; D051381:Rats
D005635:Fructosediphosphates; D006598:Hexosediphosphates; D007328:Insulin; D007527:Isoenzymes; D000249:Adenosine Monophosphate; C027652:fructose 2,6-diphosphate; D006597:Fructose-Bisphosphatase; C029063:fructose-1,6-diphosphate; D004837:Epinephrine