Focal contacts of normal and RSV-transformed quail cells. Hypothesis of the transformation-induced deficient maturation of focal contacts.
Morphology and distribution of cell-substrate contacts and their association with microfilament bundles in normal and RSV-transformed quail fibroblasts (16Q line) were studied by indirect immunofluorescence. The focal contacts were visualized by antibody exclusion method using monoclonal antibody to 80 kD serum protein adsorbed on the substratum. Embryo quail cells formed focal contacts of two morphological types: (1) small punctate; and (2) elongated large contacts. These two variants of contacts were designated respectively as dot and dash contacts. Both of focal contacts contained vinculin and alpha-actinin. Double immunofluorescence staining with polyclonal antibody to actin and monoclonal antibody to vinculin revealed that the dot contacts, in contrast to the dash ones, were not associated with microfilament bundles. The dot contacts were localized mostly near the active cell edges, while the dash contacts were found near the retracted cell edges and also under the central parts of the cell. We suppose that dot contacts represent initial structures which then can undergo maturation transforming them into dash contacts. RSV-transformed 16Q cells had predominantly the dot contacts which were not only located at the edges but also in the more central parts of the lamella. The dash contacts were present only in the minority of 16Q cells. RSV transformation is assumed to affect not the ability of cells to form initial dot contacts, but the maturation of dot contacts into dash contacts.
Bershadsky
A D
AD
Tint
I S
IS
Neyfakh
A A
AA
Vasiliev
J M
JM
D000199:Actins / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D001358:Avian Sarcoma Viruses; D002450:Cell Communication; D002460:Cell Line; D002472:Cell Transformation, Viral; D003370:Coturnix / Q000196:embryology*; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D009124:Muscle Proteins / Q000032:analysis; D011784:Quail / Q000196:embryology*; D016596:Vinculin
D000199:Actins; D000911:Antibodies, Monoclonal; D009124:Muscle Proteins; D016596:Vinculin