A novel tumor-associated molecular species of 5'-nucleotide phosphodiesterase.
5'-Nucleotide phosphodiesterase (5'-NPDase) was partially purified from plasma membranes of murine lymphoma L5178Y. The enzyme was inactivated by N-ethylmaleimide and Zn2+, but stabilized by dithiothreitol, suggesting that it is an SH enzyme. The enzyme, Km 1.54 mM, pI 5.8 and MW 23k, differs from liver 5'-NPDase in MW, Km and sensitivity to some inhibitors. On the contrary, 5'-NPDase, derived from normal mouse organs, is similar to the liver enzyme. The results suggest that tumor cells possess a novel molecular species of 5'-NPDase.
Fukazawa
H
H
Suzuki
H
H
Tanaka
N
N
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D007700:Kinetics; D007940:Leukemia L5178 / Q000201:enzymology*; D007942:Leukemia, Experimental / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D051379:Mice; D008811:Mice, Inbred DBA; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000201:enzymology; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D014018:Tissue Distribution
D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I