title: Human papillomavirus type 16 recombinant DNA is maintained as an autonomously replicating episome in monkey kidney cells.
abstract: Human papillomavirus type 16 (HPV16) DNA cloned in the expression vector pSV2-neo has been shown to be maintained in transfected monkey kidney cells as an autonomously replicating episome at a level of 2 to 10 copies per cell. Integration of pSV2-neo/HPV16 DNA with the host genome occurred in transfected mouse fibroblasts. Neither type of cell appeared to be phenotypically transformed.
authors.forename: P M
authors.initials: PM
authors.lastname: Chesters
authors.forename: D J
authors.initials: DJ
authors.lastname: McCance
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004261:DNA Replication; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D000882:Haplorhini; D007668:Kidney / Q000382:microbiology; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral