Vasopressin stimulates inositol phospholipid metabolism in rat medulla oblongata in vivo.
We have investigated whether microinjections of arginine-vasopressin (AVP) into the nucleus tractus solitarii (NTS) of rats results in the stimulation of inositol phospholipid metabolism, a biochemical event related to the activation of V1-type receptors. We found that AVP, but not the related peptides oxytocin or deamino-D-AVP, increases the incorporation of [3H]myoinositol into lipids and the accumulation of labelled inositol phosphates in the dorsomedial medulla oblongata of lithium-treated rats. These effects were abolished by local pretreatment with D-(CH2)5-Tyr(Me)-AVP, a specific antagonist of V1-receptors. It is concluded that the effects of AVP in the NTS of rats are likely to be due to an action on specific receptors (V1-like) and subsequent activation of polyphosphoinositide turnover.
Moratalla
R
R
Medical Unit, Charing Cross and Westminster Medical School, London, U.K.
Vallejo
M
M
Lightman
S L
SL
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D007295:Inositol Phosphates / Q000378:metabolism*; D008297:Male; D008526:Medulla Oblongata / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000187:drug effects / Q000502:physiology; D017483:Receptors, Vasopressin; D013403:Sugar Phosphates / Q000378:metabolism*
D007295:Inositol Phosphates; D010743:Phospholipids; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D013403:Sugar Phosphates; D001127:Arginine Vasopressin