Intermediate forms of human beta-N-acetylhexosaminidase lack activity towards 4-methylumbelliferyl beta-N-acetylglucosaminide 6-sulphate.
4-Methylumbelliferyl beta-N-acetylglucosaminide 6-sulphate was purified from a mixture containing its unsulphated precursor. The substrate was used to test for the presence of functional alpha-subunits in 'intermediate' forms of human beta-N-acetylhexosaminidase in samples of normal and pregnancy serum and in extracts of placenta and lymphocytes from a patient with common acute lymphoblastic leukaemia. Intermediate forms in these samples had no activity towards 4-methylumbelliferyl beta-N-acetylglucosaminide 6-sulphate, indicating that they lack alpha-subunits.
Beccari
T
T
Istituto di Chimica Biologica, Facoltà di Scienze MM.FF.NN, Università di Perugia, Italy.
Emiliani
C
C
Hosseini
R
R
Orlacchio
A
A
Stirling
J L
JL
D002848:Chromatography, DEAE-Cellulose; D005260:Female; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D007945:Leukemia, Lymphoid / Q000097:blood / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D014468:Umbelliferones / Q000378:metabolism*; D001619:beta-N-Acetylhexosaminidases / Q000097:blood / Q000378:metabolism*
D007527:Isoenzymes; D014468:Umbelliferones; D006923:Hymecromone; C043647:4-methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-beta-glucopyranoside; D001619:beta-N-Acetylhexosaminidases