title: Down's syndrome and acute myelofibrosis. Time study of DNA content during the progression to leukemia.
abstract: Flow cytometry (FCM) for the determination of cellular DNA content was performed on multiple bone marrow biopsy specimens from a 3-year-old boy with Down's syndrome and myelofibrosis. A rapidly fatal acute nonlymphocytic leukemia developed within 3 months after initial bone marrow evaluation. The clinical and morphologic changes corresponded to the development of aneuploidy as determined by FCM and cytogenetic analysis. These findings support the clinical observations of the premalignant potential of myelofibrosis in Down's syndrome.
authors.affiliation: Department of Pediatrics, Medical College of Georgia, Augusta 30912.
authors.forename: C G
authors.initials: CG
authors.lastname: Pantazis
authors.forename: V C
authors.initials: VC
authors.lastname: McKie
authors.forename: H
authors.initials: H
authors.lastname: Sabio
authors.forename: P C
authors.initials: PC
authors.lastname: Davis
authors.forename: W C
authors.initials: WC
authors.lastname: Allsbrook
mesh_terms: D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000032:analysis / Q000473:pathology; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000032:analysis*; D004314:Down Syndrome / Q000235:genetics*; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D008297:Male; D011289:Preleukemia / Q000235:genetics*; D055728:Primary Myelofibrosis / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm