Evidence for dopamine D-2 receptors on cholinergic interneurons in the rat caudate-putamen.
The aziridinium ion of ethylcholine (AF64A) is a neurotoxin that has demonstrated selectivity for cholinergic neurons. Unilateral stereotaxic injection of AF64A into the caudate-putamen of rats, resulted in a decrease in dopamine D-2 receptors as evidenced by a decrease in [3H]-sulpiride binding. Dopamine D-1 receptors, labeled with [3H]-SCH 23390, were unchanged. The efficacy of the lesion was demonstrated by the reduction of Na+-dependent high affinity choline uptake sites labeled with [3H]-hemicholinium-3. These data indicate that a population of D-2 receptors are postsynaptic on cholinergic interneurons within the striatum of rat brain.
Dawson
V L
VL
Department of Psychiatry, University of Utah Medical Center, Salt Lake City 84132.
Dawson
T M
TM
Filloux
F M
FM
Wamsley
J K
JK
D000818:Animals; D001388:Aziridines / Q000494:pharmacology; D002421:Caudate Nucleus / Q000187:drug effects / Q000378:metabolism*; D002794:Choline / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D006426:Hemicholinium 3 / Q000378:metabolism; D007395:Interneurons / Q000378:metabolism*; D008297:Male; D011699:Putamen / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000187:drug effects / Q000378:metabolism*; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D013469:Sulpiride / Q000378:metabolism
D001388:Aziridines; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D006426:Hemicholinium 3; D013469:Sulpiride; C044894:ethylcholine aziridinium; D002794:Choline