[Effect of vanadate on the vacuolar ATPase of Acer pseudoplatanus].
The ATPase of vacuoles isolated from Acer pseudoplatanus cells is strongly inhibited by vanadate, a specific inhibitor of plasma membrane ATPase. The degree of inhibition depends upon the ionic composition, ATP and magnesium concentrations of the reaction medium and the inhibition is reversed by EDTA which complexes vanadate. In absence of factor which may interfere with the effectiveness of inhibitor, vanadate inhibits the ATPase non-competitively.
Montrichard
F
F
Laboratoire de Biochimie, UA CNRS 531, Faculté des Sciences et des Techniques, Besançon.
Magnin
T
T
Pugin
A
A
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000643:Ammonium Chloride / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D008278:Magnesium Sulfate / Q000494:pharmacology; D010944:Plants / Q000648:ultrastructure; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014617:Vacuoles / Q000201:enzymology*; D014638:Vanadates / Q000494:pharmacology*
D000643:Ammonium Chloride; D014638:Vanadates; D012965:Sodium Chloride; D011189:Potassium Chloride; D008278:Magnesium Sulfate; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D000251:Adenosine Triphosphatases
Effet du vanadate sur l'ATPase vacuolaire d'Acer pseudoplatanus.