Pleiotropic effects of the Bcg gene. II. Genetic restriction of responses to mitogens and allogeneic targets.
The response of Bcgr and Bcgs spleen cells to allogeneic Ag, mitogens, and in a system of oxidative mitogenesis using neuraminidase and galactose oxidase was investigated in two Bcg congenic systems. The Bcgr macrophages supported the MLR across H-2 barrier much better than the Bcgs macrophages. At sub-optimal or optimal doses of mitogens Bcgr mice were higher responders than their Bcgs counterparts. The superior response of Bcgr spleen cells to Con A was further investigated with the aim of identifying the population expressing this phenotype. T cells of either Bcgr or Bcgs type showed equal ability to respond to Con A in the presence of macrophages. Purified splenic macrophages from Bcgr mice contained a significantly greater percentage of Ia+-bearing macrophages compared to Bcgs mice. Splenic macrophages of the Bcgr type were more efficient than their Bcgs counterparts at restoring the Con A response of accessory cell-depleted spleen cells. Resident peritoneal macrophages as well as splenic dendritic cells from Bcgr and Bcgs mice were equally efficient at restoring this response. Glutaraldehyde-fixed Bcgr splenic macrophages were shown to be more efficient than the Bcgs cells at replenishing the response of Con A-unresponsive spleen cells when supplemented with IL-1.
Denis
M
M
Département de Microbiologie et d'Immunologie, Université de Montréal, Québec, Canada.
Buschman
E
E
Forget
A
A
Pelletier
M
M
Skamene
E
E
D000818:Animals; D003208:Concanavalin A; D000949:Histocompatibility Antigens Class II; D007113:Immunity, Innate; D007519:Isoantigens / Q000235:genetics*; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008934:Mitogens; D010101:Oxygen Consumption; D010835:Phytohemagglutinins; D013154:Spleen; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D008934:Mitogens; D010835:Phytohemagglutinins; D003208:Concanavalin A