[Proteinase participation in chromosome radiation damage. Research on the action of phenylmethylsulfonyl fluoride].
Phenylmethylsulfonyl fluoride, a chromatin proteinase inhibitor, caused a nearly twofold diminution of the cytogenetic injury and a twofold increase in the rate of DNA repair in gamma-irradiated (3-15 Gy) Chinese hamster fibroblasts. The effect of the inhibitor was mainly exhibited by a rapidly repaired (for 15-20 min) component of the cytogenetic damage. A simultaneous treatment with phenylmethylsulfonyl fluoride and nicotinamide did not influence the effect of the proteinase inhibitor under study. The results obtained are indicative of poly (ADP-ribosylation)-independent contribution of chromatin proteinases to radiation-induced chromosome mutagenesis.
Aptikaeva
G F
GF
Rozanova
O M
OM
Saugabaeva
K M
KM
Ganassi
E E
EE
D000246:Adenosine Diphosphate Ribose / Q000096:biosynthesis; D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D002875:Chromosomes / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D010450:Endopeptidases / Q000528:radiation effects*; D009536:Niacinamide / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D011480:Protease Inhibitors; D013450:Sulfones / Q000494:pharmacology*
D002843:Chromatin; D011480:Protease Inhibitors; D013450:Sulfones; D000246:Adenosine Diphosphate Ribose; D009536:Niacinamide; D010664:Phenylmethylsulfonyl Fluoride; D010450:Endopeptidases
Uchastie proteinaz v radiatsionnom povrezhdenii khromosom. Issledovanie deĭstviia fenilmetilsul'fonilftorida.