Enhancement of DNA-mediated gene transfer by high-Mr carrier DNA in synchronized CV-1 cells.
Synchronized CV-1 cells were transfected with SV40 (simian virus 40) DNA-calcium phosphate co-precipitates. In the presence of carrier DNA, the transfection efficiency of SV40 DNA was decreased 5-fold in S-phase cells and was increased 4-fold in preparations of mitotically enriched cells as compared with asynchronous controls. No difference was observed when carrier DNA was omitted, when cells had progressed through S-phase and into G2-phase, or when the infectivity of cells to intact SV40 virus was tested. These results highlight the importance of cell-cycle-dependent factors on DNA-mediated gene transfer.
Strain
A J
AJ
Wallace
W A
WA
Wyllie
A H
AH
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D004247:DNA / Q000494:pharmacology; D004279:DNA, Viral / Q000235:genetics*; D006918:Hydroxyurea / Q000494:pharmacology; D008938:Mitosis; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection / Q000187:drug effects
D004279:DNA, Viral; D004247:DNA; D006918:Hydroxyurea