Further characterization of cardiodigin, Na+, K+-ATPase inhibitor extracted from mammalian tissues.
This study was undertaken to characterize endogenous digitalis-like activity in water extract from mammalian tissues. Prepurified samples obtained from guinea-pig heart were analysed by reverse-phase HPLC using an acetonitrile gradient. The eluent was assayed for its activity as inhibitor of human heart Na+, K+-ATPase and digoxin-like immunoreactivity. Both activities were recovered in the same fraction after two successive chromatographic steps. These results provide further evidence for the presence of an endogenous digitalis-like factor, cardiodigin, in mammalian heart.
Fagoo
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Godfraind
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D000818:Animals; D001688:Biological Products / Q000302:isolation & purification* / Q000494:pharmacology; D002301:Cardiac Glycosides; D002851:Chromatography, High Pressure Liquid; D004077:Digoxin / Q000032:analysis; D006168:Guinea Pigs; D009206:Myocardium / Q000032:analysis*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D001688:Biological Products; D002301:Cardiac Glycosides; D004077:Digoxin; C045241:cardiodigin; D000254:Sodium-Potassium-Exchanging ATPase