Recognition of mos-related proteins with an antiserum to a peptide of the v-mos gene product.
A mos-specific antiserum was generated by injection of rabbits with a peptide predicted from the sequence of the v-mos gene of Moloney murine sarcoma virus (MuSV) strain 124. The peptide is composed of amino acids 37-55 (cyclized at the cysteine residues) conjugated to keyhole limpet haemocyanin. This serum [anti-mos(37-55c)] specifically recognized p37mos in MuSV-124 acutely infected NIH-3T3 cells, P85gag-mos in 6m2 cells, an NRK clone infected with the temperature-sensitive mutant (ts110) of Moloney MuSV, and P100gag-mos in 54-5A4 cells, an NRK clone infected with a spontaneous revertant of ts110. An additional protein of Mr 55000 from uninfected cells was recognized by this serum. Reactivity of the serum toward the v-mos-containing proteins and the 55K protein was completely inhibited by prior incubation with free peptide. The 55K protein was not recognized by antisera made from synthetic peptides prepared from the C-terminal eight or 12 amino acids of v-mos.
Gallick
G E
GE
Sparrow
J T
JT
Singh
B
B
Maxwell
S A
SA
Stanker
L H
LH
Arlinghaus
R B
RB
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D002472:Cell Transformation, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D015683:Gene Products, gag; D007106:Immune Sera; D051379:Mice; D008970:Molecular Weight; D008980:Moloney murine sarcoma virus / Q000032:analysis* / Q000502:physiology; D011233:Precipitin Tests; D011506:Proteins / Q000276:immunology; D051381:Rats; D009053:Sarcoma Viruses, Murine / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D015683:Gene Products, gag; D007106:Immune Sera; D011506:Proteins; D014764:Viral Proteins