title: Morphological and functional characteristics of rat Leydig cells isolated on Percoll gradients: is Leydig cell heterogeneity in vitro an artifact?
abstract: Rat testicular intertubular cells have been isolated on Percoll density gradients. Detailed light and electron microscopic studies have determined the sedimentation positions for Leydig cells, macrophages, fibroblasts, endothelial cells, germ cells and residual bodies. Stereological techniques have been utilized to determine the number of cells in the region of the gradient where Leydig cells sediment. Morphologically intact Leydig cells were present in the more dense region of the gradient (1.0590-1.0900 g/ml), and they responded to hCG stimulation with an 11-fold increase in testosterone production and contained LH/hCG receptors. Leydig cells in the less dense region of the gradient (1.0440-1.0589 g/ml) secreted less testosterone and contained less hCG receptors than those obtained from denser regions. However, the morphological studies described herein provide evidence for the first time that the majority of these less functional 'Leydig cells' from the lighter region of the gradients do not contain a nucleus and represent pieces of Leydig cell cytoplasm with variable size, shape and complement of organelles.
authors.forename: A O
authors.initials: AO
authors.lastname: Laws
authors.forename: N G
authors.initials: NG
authors.lastname: Wreford
authors.forename: D M
authors.initials: DM
authors.lastname: de Kretser
mesh_terms: D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D004727:Endothelium / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D007985:Leydig Cells / Q000166:cytology* / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D011205:Povidone; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism; D011974:Receptors, LH; D012671:Seminiferous Tubules / Q000166:cytology; D012822:Silicon Dioxide; D013739:Testosterone / Q000096:biosynthesis
chemical_list: D011956:Receptors, Cell Surface; D011974:Receptors, LH; D013739:Testosterone; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone