title: In situ localization of the major capsid protein during lytic infection by herpes simplex virus.
abstract: The intracellular localization of the major capsid protein (ICP5) of herpes simplex virus was studied during virogenesis. Except for a brief period at the onset of synthesis, this protein was found almost exclusively inside the nucleus. Its localization was not at random since 80% was tightly bound to the nuclear matrix as early as 4 h after infection. Discrete modifications of the fluorescence pattern occurred in an orderly fashion during the progression of the infection. Immunoelectron microscopic studies using Protein A-gold labelling demonstrated that this protein is synthesized on cytoskeleton-bound polyribosomes and accumulates in the central part of the nucleus where formation of viral capsids occurs; no gold particles were found in association with the peripheral chromatin or with the nucleolus.
authors.forename: V
authors.initials: V
authors.lastname: Bibor-Hardy
authors.forename: A
authors.initials: A
authors.lastname: Dagenais
authors.forename: R
authors.initials: R
authors.lastname: Simard
mesh_terms: D000818:Animals; D002213:Capsid / Q000032:analysis* / Q000378:metabolism; D006224:Cricetinae; D006561:Herpes Simplex / Q000378:metabolism; D018139:Simplexvirus / Q000032:analysis*