Independence with respect to Ca2+ changes of the neutrophil respiratory and secretory response to exogenous phospholipase C and possible involvement of diacylglycerol and protein kinase C.
Exogenous phospholipase C induces in human neutrophils the activation of a respiratory burst, measured as O2 consumption and O-2 production, and of secretion of specific granules, measured as release of vitamin B-12 binding protein. The secretory response is minimal and follows the onset of the respiratory response. Studies carried out using cells prelabeled with [3H]glycerol and 32P on the molecular mechanism of the stimulations demonstrate that the effects are dependent on the formation of diacylglycerol by hydrolysis of different classes of glycerophospholipids. They are, however, independent of the activation of a 'phosphoinositide turnover' as occurs in cells stimulated with fMet-Leu-Phe. Furthermore, the respiratory and secretory responses to exogenous phospholipase C are not associated with modifications of cytosolic Ca2+ concentration, measured with the Quin-2 method, and of the release of bound Ca2+, measured with the membrane probe, chlorotetracycline. Apart from a quantitative difference, mostly regarding the ratio of the intensity of the respiratory and secretory responses, the effects caused by exogenous phospholipase C are qualitatively similar to those induced by phorbol myristate acetate and are probably linked to an involvement of protein kinase C, activated by diacylglycerol liberated in the plasma membrane.
Grzeskowiak
M
M
Della Bianca
V
V
De Togni
P
P
Papini
E
E
Rossi
F
F
D002118:Calcium / Q000502:physiology*; D004075:Diglycerides / Q000502:physiology*; D005089:Exocytosis; D005989:Glycerides / Q000502:physiology*; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology*; D010101:Oxygen Consumption; D010716:Phosphatidylinositols / Q000502:physiology; D010740:Phospholipases / Q000502:physiology*; D010743:Phospholipids / Q000378:metabolism; D011493:Protein Kinase C; D011494:Protein Kinases / Q000502:physiology*; D010738:Type C Phospholipases / Q000502:physiology*
D004075:Diglycerides; D005989:Glycerides; D008563:Membrane Lipids; D010716:Phosphatidylinositols; D010743:Phospholipids; D011494:Protein Kinases; D011493:Protein Kinase C; D010740:Phospholipases; D010738:Type C Phospholipases; D002118:Calcium