Enhancement of suppressor T cell activity by injection of anti-IFN-gamma monoclonal antibody.
In the present study, the contribution of IFN-gamma to the generation of helper activity in mice was investigated by use of anti-mouse IFN-gamma rat mAB (AN 18.17.24). This mAb was alum precipitated and injected i.p. before or after carrier priming. Results show that spleen cell helper activity is markedly inhibited by anti-IFN-gamma mAb injection. This inhibition is time and dose dependent, and counteracted by IFN-gamma administration. Thus, the anti-IFN-gamma mAb appears to inhibit helper cell activity by neutralization of the IFN-gamma required for the antibody response. Moreover, AN 18.17.24 mAb injection results in increased activation of Lyt-2+ T cells which markedly suppress Th activity. These findings altogether indicate that besides the activation of macrophages and Th, IFN-gamma seems to exert a negative interference in suppressor T lymphocyte circuits and, as a consequence, to inhibit immunosuppression.
Frasca
D
D
Laboratory of Pathology, ENEA C.R.E. Casaccia, Roma, Italy.
Adorini
L
L
Landolfo
S
S
Doria
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D000950:Antigens, Ly; D004306:Dose-Response Relationship, Immunologic; D006462:Hemolytic Plaque Technique; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D010641:Phenotype; D051381:Rats; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D007166:Immunosuppressive Agents; D014301:Trinitrobenzenes; D007371:Interferon-gamma