Biochemically stimulated release of atrial natriuretic factor from heart-lung preparation of Dahl rats.
We investigated the effect of sodium chloride and adrenergic agents on the release of atrial natriuretic factor (ANF) using working heart-lung preparations from Dahl salt-hypertension sensitive (S) and Dahl salt-hypertension resistant (R) rats. High concentrations of NaCl moderately increased ANF release, but this was attributed to small increases in left atrial pressure rather than to a direct effect of NaCl on ANF release; S and R rats responded similarly. Neither isoproterenol (beta 1 + beta 2 agonist) nor clonidine (alpha 2 agonist) had any effect on ANF release in the heart-lung preparation. In contrast, phenylephrine (alpha 1 agonist) stimulated ANF release. This could not be accounted for by change in atrial pressure and appeared to be a direct effect. S and R rats both released ANF in response to phenylephrine, but there was a modest tendency for hypertensive S rats to release more ANF than normotensive R rats, which is consistent with previous data on mechanically induced (atrial stretch) ANF release in these strains.
Onwochei
M O
MO
Department of Medicine, Medical College of Ohio, Toledo 43699.
Rapp
J P
JP
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D003000:Clonidine / Q000494:pharmacology; D006439:Hemodynamics / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D015016:Yohimbine / Q000494:pharmacology
D010656:Phenylephrine; D015016:Yohimbine; D012965:Sodium Chloride; D009320:Atrial Natriuretic Factor; D003000:Clonidine