title: Enzymatic detection of phospholipid biosynthetic enzymes after electroblotting.
abstract: The membrane-associated enzymes phosphatidylinositol synthase (CDPdiacylglycerol:myo-inositol 3-phosphatidyltransferase; EC 2.7.8.11) and phosphatidylserine synthase (CDPdiacylglycerol:L-serine O-phosphatidyltransferase; EC 2.7.8.8) from Saccharomyces cerevisiae were detected enzymatically after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotting. Enzyme activities were measured on nitrocellulose blots by using pure enzyme preparations as well as Triton X-100-solubilized membranes. Phosphatidylinositol synthase activity migrated to Mr 34,000, and phosphatidylserine synthase activity migrated to Mr 23,000.
authors.forename: M A
authors.initials: MA
authors.lastname: Poole
authors.forename: A S
authors.initials: AS
authors.lastname: Fischl
authors.forename: G M
authors.initials: GM
authors.lastname: Carman
mesh_terms: D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D010770:Phosphotransferases / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D017855:Transferases (Other Substituted Phosphate Groups)
chemical_list: D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase