title: Cosecretion of peptides derived from gamma-human atrial natriuretic polypeptide in normal volunteers and patients with essential hypertension and adrenal disorders.
abstract: Using two radio-immunoassays for N-terminal and C-terminal fragments of human atrial natriuretic polypeptide (ANP) precursor, gamma-hANP [human atrial natriuretic factor-(1-126)], that is gamma-hANP(1-25) [human atrial natriuretic factor-(1-25)] and alpha-hANP [human atrial natriuretic factor-(99-126)], we studied the secretion of gamma-hANP-derived peptides into circulation from the heart in normal subjects and patients with essential hypertension and adrenal disorders. Volume expansion with 2 litres physiological saline increased plasma gamma-hANP(1-25)-like immunoreactivity concomitantly with plasma alpha-hANP-like immunoreactivity in normal subjects. Infusion of angiotensin II (20 ng/kg per min) or noradrenaline (200 ng/kg per min) also caused a parallel increase in plasma gamma-hANP(1-25)-like and alpha-hANP-like immunoreactivity. Plasma gamma-hANP(1-25)-like immunoreactivity levels were changed together with alpha-hANP-like immunoreactivity in patients with essential hypertension and adrenal disorders. These results indicate that gamma-hANP-derived peptides, alpha-hANP and the 10-k N-terminal fragment of gamma-hANP (N-peptide) are cosecreted from the heart and that the simultaneous measurement of N-peptide and alpha-hANP serves as an indicator of the cardiac endocrine function. The significance of N-peptide as a hormone must await further clarification.
authors.affiliation: Department of Medicine, Kyoto University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Sugawara
authors.forename: K
authors.initials: K
authors.lastname: Nakao
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Mukoyama
authors.forename: H
authors.initials: H
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Hosoda
authors.forename: G
authors.initials: G
authors.lastname: Shirakami
authors.forename: N
authors.initials: N
authors.lastname: Morii
mesh_terms: D000224:Addison Disease / Q000378:metabolism; D000307:Adrenal Gland Diseases / Q000378:metabolism*; D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000804:Angiotensin II / Q000494:pharmacology; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001810:Blood Volume; D003480:Cushing Syndrome / Q000378:metabolism*; D006801:Humans; D006929:Hyperaldosteronism / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism*; D007118:Immunoassay; D010673:Pheochromocytoma / Q000378:metabolism
chemical_list: D000804:Angiotensin II; D009320:Atrial Natriuretic Factor