title: Effects of vasopressin on phosphoinositides and prostaglandin production in cultured mesangial cells.
abstract: We studied the effects of vasopressin on phospholipid metabolism in mesangial cells and the temporal relationship of these changes to prostaglandin production. The changes included: 1) increased breakdown of phosphatidylinositol (PI), 2) increased breakdown of diphosphoinositide (PI-P) and triphosphoinositide (PI-P2), 3) an increase in the mass of diglyceride and phosphatidic acid, 4) increased synthesis, first of PI-P and PI-P2, then phosphatidic acid, and, finally, PI, and 5) increased water-soluble inositol phosphates. Vasopressin treatment resulted in a significant increase (90%) in the mass of phosphatidic acid and a smaller (13%) decline in the mass of PI. Changes in diglyceride were seen following 45 s of treatment with vasopressin. Alterations of phosphoinositide metabolism were seen as early as 45 s and continued for up to 5 h following hormone exposure. By contrast, prostaglandin production declined after 30 min. These observations on vasopressin-stimulated metabolism in cultured mesangial cells may provide a basis for an understanding of the functional changes that follow hormone exposure.
authors.forename: D A
authors.initials: DA
authors.lastname: Troyer
authors.forename: J I
authors.initials: JI
authors.lastname: Kreisberg
authors.forename: D W
authors.initials: DW
authors.lastname: Schwertz
authors.forename: M A
authors.initials: MA
authors.lastname: Venkatachalam
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism; D015232:Dinoprostone; D005227:Fatty Acids / Q000378:metabolism; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D011453:Prostaglandins / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical; D013997:Time Factors; D014667:Vasopressins / Q000494:pharmacology*
chemical_list: D004075:Diglycerides; D005227:Fatty Acids; D010712:Phosphatidic Acids; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; D011453:Prostaglandins; D011458:Prostaglandins E; C023687:diphosphoinositides; C018803:triphosphoinositide; D014667:Vasopressins; D015232:Dinoprostone