Platelet membrane molecular organization: relationship with membrane bound calcium.
The fatty acid spin label 5 nitroxide stearate has been used to determine the membrane organization changes induced by platelet aggregation. A decrease in order is observed with thrombin, even in the presence of EDTA, when aggregation is inhibited. Conversely, after aggregation by the calcium ionophore A23187 the rigidity of the phospholipids is not modified. These effects are discussed in relation to the release of membrane bound calcium induced by thrombin.
Rendu
F
F
Viret
J
J
Daveloose
D
D
Levy-Toledano
S
S
Leterrier
F
F
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D066298:In Vitro Techniques; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000097:blood; D010974:Platelet Aggregation / Q000187:drug effects; D013917:Thrombin / Q000494:pharmacology
D008563:Membrane Lipids; D000001:Calcimycin; D004492:Edetic Acid; D013917:Thrombin; D002118:Calcium