Immunofluorescence detection of liver cell membrane autoantibodies using PLC/PRF/5 cells.
Detection of liver cell membrane autoantibodies is routinely performed by immunofluorescence testing of patient sera on rabbit hepatocyte suspensions. We have investigated the possible use of cells from the PLC/PRF/5 human hepatoma cell-line. These cells were employed as substrate in an immunofluorescence test which was compared with the conventional rabbit hepatocyte assay. We found a close correlation between the results obtained with these different substrates on visual reading. We furthermore compared visual reading of immunofluorescence preparations with flow-cytometrical analysis of immunostained cell suspensions. The results with these different methods were largely confirmatory. The PLC/PRF/5 cells are easily available and should therefore be regarded as a highly valuable new substrate for detection of liver cell membrane autoantibodies. Flow cytometry appears to be a technically simple and reliable method for quantitative analysis.
de Koning
R W
RW
Daemen
M
M
Schutte
B
B
Geertzen
H G
HG
Blijham
G H
GH
Bosman
F T
FT
D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002908:Chronic Disease; D005455:Fluorescent Antibody Technique; D006505:Hepatitis / Q000276:immunology*; D006801:Humans; D008099:Liver / Q000276:immunology*; D008113:Liver Neoplasms / Q000276:immunology*; D011817:Rabbits
D001323:Autoantibodies