Nucleotide sequence of a functional cDNA for human thymidylate synthase.
We have determined the nucleotide sequence of a cDNA clone, pcHTS-1, encoding human thymidylate synthase (5,10-methylenetetrahydrofolate: dUMP C-methyltransferase, EC 2.1.1.45) which was previously isolated from a human fibroblast expressible cDNA library and functional in mouse cells. The 1.6 kilobase cDNA insert of pcHTS-1 encodes a subunit protein of 313 amino acid (Mr = 35,706) and its predicted amino acid sequence is highly conserved in many regions including folylpolyglutamate and 5-fluoro-2'-deoxyuridylate binding sites, when compared with those of Lactobacillus casei, Escherichia coli, and bacteriophage T4. The cDNA contains in its 5'-untranslated region a triple tandemly repeated sequence consisting of 90 nucleotides, which starts immediately upstream of the ATG initiator codon, is very high in G+C content (80%), and can form three possible interconvertible stem-loop structures.
Takeishi
K
K
Kaneda
S
S
Ayusawa
D
D
Shimizu
K
K
Gotoh
O
O
Seno
T
T
D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D005809:Genes, Regulator; D006801:Humans; D008780:Methyltransferases / Q000235:genetics*; D009690:Nucleic Acid Conformation; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D013940:Thymidylate Synthase / Q000235:genetics*
D011061:Poly A; D004247:DNA; D008780:Methyltransferases; D013940:Thymidylate Synthase; D004262:DNA Restriction Enzymes