title: Interaction between prostaglandin E1 and forskolin in modulation of cyclic AMP levels in rat epididymal adipocytes.
abstract: The interaction between the diterpene, forskolin and prostaglandin E1 on cAMP levels in isolated adipocytes of the rat was examined. Various doses of Prostaglandin E1 in the presence of 1-methyl-3-isobutylxanthine (2 mM) blocked the increase in the intracellular level of cAMP elicited by forskolin (0.1 microM or 5 microM). Interestingly, in the absence of 1-methyl-3-isobutylxanthine (or an adenosine blocker), or in the presence of 1-methyl-3-isobutylxanthine (2 mM) and forskolin (30 microM), prostaglandin E1 (1 nM-100 microM) exhibited biphasic dose-response kinetics. The stimulatory effect of PGE1 (greater than 1 microM) in presence of forskolin (30 microM) may be a consequence of prostaglandin receptor modification following endogeneous release of adenosine and prostaglandins by adipocytes stimulated to high levels of cAMP accumulation due to 30 microM forskolin.
authors.forename: S H
authors.initials: SH
authors.lastname: Fatemi
mesh_terms: D000243:Adenosine Deaminase / Q000494:pharmacology; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000527:Alprostadil; D000818:Animals; D002478:Cells, Cultured; D005576:Colforsin; D000242:Cyclic AMP / Q000378:metabolism*; D004224:Diterpenes / Q000494:pharmacology*; D004357:Drug Synergism; D004822:Epididymis; D004837:Epinephrine / Q000494:pharmacology; D008297:Male; D008469:Meclofenamic Acid / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004224:Diterpenes; D011458:Prostaglandins E; D005576:Colforsin; D008469:Meclofenamic Acid; D000242:Cyclic AMP; D000243:Adenosine Deaminase; D000527:Alprostadil; D004837:Epinephrine