Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product.
We have mapped a transcriptional regulatory sequence within the 1.0 kb noncoding region of the bovine papilloma virus (BPV-1) genome, using an enhancer dependent expression vector for chloramphenicol acetyltransferase. This transcriptional regulatory element works independently of position and orientation, and its function is significantly augmented in BPV-1 transformed C127 cells and in monkey CV-1 cells acutely transfected with plasmids expressing BPV-1 early gene products. Using defined deletion mutants of the BPV-1 DNA and full-length viral cDNAs expressed from an SV40 early promoter, we demonstrate that the expression of this trans-activating factor maps to the 3' open reading frames of the viral transforming region. A premature termination codon engineered into the E2 ORF eliminates expression of this diffusible transactivation function establishing the E2 gene product as the diffusible trans-activating factor.
Spalholz
B A
BA
Yang
Y C
YC
Howley
P M
PM
D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics* / Q000502:physiology; D002417:Cattle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D015500:Chloramphenicol O-Acetyltransferase; D002522:Chlorocebus aethiops; D004261:DNA Replication; D004274:DNA, Recombinant; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D005814:Genes, Viral; D051379:Mice; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection; D014779:Virus Replication
D004274:DNA, Recombinant; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase