[Characterization, in the guinea pig, of a hepatic cytosol protein binding dehydroepiandrosterone sulfate and estrone sulfate].
A protein which binds dehydroepiandrosterone sulfate and estrone sulfate was detected in the cytosolic fraction of female Guinea-pig liver. It is characterized by a molecular mass of 14,400 Da, its affinity for DHEA sulfate (KD = 8.8 microM) and estrone sulfate (KD = 8.5 microM), and its lack of affinity for free steroids such as dehydroepiandrosterone or estrone. It is eluted by gel filtration on Sephadex G-50 simultaneously with the inhibitor of microsomal DHEA sulfatase recently described by some of us. This protein could be implicated in the intracellular transport or in the metabolism of sulfated steroids.
Adessi
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Unité de Biochimie hormonale et des Régulations, I.N.S.E.R.M. U. n. 98, Besançon.
Roblin
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Nicollier
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D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003600:Cytosol / Q000032:analysis; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000378:metabolism; D019314:Dehydroepiandrosterone Sulfate; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000037:antagonists & inhibitors
D002352:Carrier Proteins; D004970:Estrone; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D013429:Sulfatases; D043266:Steryl-Sulfatase; C017296:estrone sulfate
Mise en évidence, chez le cobaye, d'une protéine cytosolique hépatique liant le sulfate de déhydroépiandrostérone et le sulfate d'estrone.