title: Sequences essential for IS50 transposition. The first base-pair.
abstract: Sequences near the ends of the insertion element IS50 are essential for its transposition, probably because they serve as sites upon which the IS50-encoded transposase protein acts. To determine if these essential sequences include the first base-pair at each end of IS50 we generated 5'C to 5'G transversions at these positions. Each mutation reduced the transposition frequency to 1% to 2% of wild-type. DNA sequence analyses showed that the mutant 5'G is preserved during transposition.
authors.forename: C
authors.initials: C
authors.lastname: Sasakawa
authors.forename: S H
authors.initials: SH
authors.lastname: Phadnis
authors.forename: G F
authors.initials: GF
authors.lastname: Carle
authors.forename: D E
authors.initials: DE
authors.lastname: Berg
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004279:DNA, Viral; D009154:Mutation; D010957:Plasmids
chemical_list: D004251:DNA Transposable Elements; D004279:DNA, Viral; D004262:DNA Restriction Enzymes