Biochemical studies on 5'-nucleotidase of Schwann cells in degenerated nerve.
This report describes the partial characterization of 5'-nucleotidase (5'-AMPase) in Schwann-cell plasmalemmae (PM) prepared from degenerated cat sciatic nerve. 5'-AMPase was enriched 3.7-fold in the PM fraction over that of the crude homogenate preparation. The plant lectin concanavalin-A (Con-A) reduced Schwann cell PM 5'-AMPase activity in a concentration-dependent manner (30-600 micrograms/ml). Plasma membrane 5'-AMPase activity was maximally inhibited to 20% of control values by Con-A (400-600 micrograms/ml), and activity returned to control levels by pretreatment with the hapten sugar alpha-methyl-D-mannoside (50 mM). Equimolar concentrations of UDP and ADP (100 microM) reduced the rate of hydrolysis of labeled AMP to labeled adenosine in PM to 45% and 35% of control, respectively. This is the first study to characterize a Schwann-cell PM enzyme and demonstrates that 5'-AMPase may be used as a Schwann-cell PM marker enzyme.
Ross
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Sabri
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Spencer
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D015720:5'-Nucleotidase; D000241:Adenosine / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D002415:Cats; D002462:Cell Membrane / Q000201:enzymology; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D008297:Male; D008766:Methylmannosides / Q000494:pharmacology; D009410:Nerve Degeneration; D009708:Nucleotidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012583:Schwann Cells / Q000201:enzymology*; D012584:Sciatic Nerve / Q000201:enzymology; D014530:Uridine Diphosphate / Q000494:pharmacology
D008766:Methylmannosides; D003208:Concanavalin A; D000249:Adenosine Monophosphate; C008466:methylmannoside; D014530:Uridine Diphosphate; D000244:Adenosine Diphosphate; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000241:Adenosine