hst-1 transforming protein: expression in silkworm cells and characterization as a novel heparin-binding growth factor.
A protein encoded by an hst-1 transforming gene was expressed in silkworm-derived BmN cells and secreted into the culture medium using a recombinant baculovirus vector. The strong affinity for heparin of the secreted protein made it possible to purify the hst-1 protein to homogeneity in a two-step procedure. The purified hst-1 protein has a molecular weight of 18,000 and stimulates both DNA synthesis in NIH3T3 cells and human umbilical vein endothelial cell proliferation. In addition, morphological changes and anchorage-independent growth of NIH3T3 cells are induced by this product. These results show that the hst-1 transforming protein is a novel heparin-binding growth factor as predicted by nucleotide sequence analysis.
Miyagawa
K
K
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Sakamoto
H
H
Yoshida
T
T
Yamashita
Y
Y
Mitsui
Y
Y
Furusawa
M
M
Maeda
S
S
Takaku
F
F
Sugimura
T
T
Terada
M
M
D000818:Animals; D001483:Base Sequence; D012831:Bombyx; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004261:DNA Replication / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors; D015870:Gene Expression; D005822:Genetic Vectors; D006133:Growth Substances / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D006493:Heparin / Q000235:genetics*; D008934:Mitogens / Q000235:genetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D015513:Oncogene Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D009857:Oncogenes; D011518:Proto-Oncogene Proteins; D014162:Transfection
D051519:Fibroblast Growth Factor 4; D006133:Growth Substances; D008934:Mitogens; D015345:Oligonucleotide Probes; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D005346:Fibroblast Growth Factors; D006493:Heparin