[Demonstration of calmodulin in cultured human malignant melanocytes by indirect immunofluorescence].
Using calmodulin antibody, evidence for the presence of calmodulin, a calcium-binding protein modulator of numerous enzymes was shown by indirect immunofluorescence in 9 cell lines of cultured human malignant melanocytes. Calmodulin was localized in cytoplasm and in higher concentration in the perinuclear region. These observations agree with the distribution of calmodulin-binding proteins in cytoplasm and membranes.
Dutoit
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C
Reynier
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Aquaron
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R
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism; D002147:Calmodulin / Q000032:analysis* / Q000502:physiology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D003593:Cytoplasm / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008543:Melanins / Q000096:biosynthesis; D008544:Melanocytes / Q000032:analysis* / Q000201:enzymology; D008545:Melanoma / Q000032:analysis
D002147:Calmodulin; D008543:Melanins; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000262:Adenylyl Cyclases
Mise en évidence de la calmoduline dans des mélanocytes malins humains en culture par immunofluorescence indirecte.