Comparison of virulence factors and R plasmids of Salmonella spp. isolated from healthy and ill swine.
The antibiotic resistance and virulence profiles of Salmonella spp. isolated from healthy (group 1) and ill (group 2) swine were compared. Parameters studied included colicin and siderophore production; mannose-sensitive hemagglutination of erythrocytes; resistance to the lethal effect of serum complement; resistance to antibiotics; and the transmissibility of these characteristics to recipient organisms. Group 1 (19 isolates) had 14 serotypes, and group 2 (20 isolates) had 2 serotypes. Isolates from group 2 were resistant to more antibiotics and had a greater ability to hemagglutinate erythrocytes and transfer R plasmids to recipient organisms, but a lesser ability to produce siderophore than group 1. All 39 isolates resisted the lethal effects of serum complement. Colicin was produced by 1 of 19 from group 1 and 0 of 20 from group 2. A donor Escherichia coli isolated from a pig with enteritis transferred R plasmids to 62% of group 1 and 0% of group 2 Salmonella spp. when they were used as recipient organisms. A transconjugant from the mating of donor E. coli to a group 1 Salmonella spp. was further able to pass an R plasmid to recipient E. coli and salmonellae. Plasmid isolation from group 1 yielded 1 of 19 strains with a 56-megadalton plasmid, while 20 of 20 strains from group 2 contained three to five plasmids from 2.4 to 60 megadaltons in size.
Simmons
K W
KW
Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Wooley
R E
RE
Brown
J
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001770:Blood Bactericidal Activity; D003087:Colicins / Q000096:biosynthesis; D003165:Complement System Proteins / Q000276:immunology; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks / Q000662:veterinary; D004758:Enterobactin / Q000096:biosynthesis; D006386:Hemagglutination Tests; D011815:R Factors; D012475:Salmonella / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012703:Serotyping; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000382:microbiology*; D014774:Virulence
D000900:Anti-Bacterial Agents; D003087:Colicins; D004269:DNA, Bacterial; D004758:Enterobactin; D003165:Complement System Proteins