Enzyme immunoassay of anti-glomerular basement membrane antibodies.
An immunoassay has been developed to detect anti-glomerular basement membrane (GBM) antibodies in human sera. Various plating conditions, types of microtiter plates, and the use of biotinylated or peroxidase-labeled secondary antibodies were examined. The described assay is reliable, fast, and convenient. Sera with positive reactivity in anti-GBM nephritis and Goodpasture's syndrome are readily discriminated from sera obtained from normal individuals or patients with a variety of other diseases.
Fish
A J
AJ
Kleppel
M
M
Jeraj
K
K
Michael
A F
AF
D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology; D000906:Antibodies / Q000032:analysis*; D001485:Basement Membrane / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005921:Glomerulonephritis / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007678:Kidney Glomerulus / Q000276:immunology*; D003012:Microbial Collagenase
D000906:Antibodies; D003012:Microbial Collagenase