title: Functional and structural evaluation of the vasculature of skin flaps after ischemia and reperfusion.
abstract: Free radicals and other toxic oxygen species play a role in the pathogenesis of ischemic organ damage. The abdominal skin flap has been used as a model to study the effects of superoxide dismutase on the survival of ischemic skin. We have evaluated the evolution of functional and structural injury to the vasculature after ischemic injury in superoxide dismutase-treated and control skin flaps. Ischemia was induced by creating abdominal skin flaps and occluding either the venous or both the venous and arterial blood supplies. Superoxide dismutase was administered immediately after the occlusion was released. At 1 hour of reflow, erythrocyte stasis, platelet deposition, neutrophil adherence, and injury to the endothelium of the large vessels and of the microvasculature were evident. The blood flow in the ischemic skin was only 3 percent of normal. Superoxide dismutase caused no change in the ultrastructure of the vasculature and a marginal decrease in vascular permeability in the ischemic skin at 1 hour of reflow. Increased fluorescent staining of the skin was evident after 24 hours of reflow in the superoxide dismutase-treated flaps. These findings indicate that injury to vascular endothelium by ischemia and reperfusion plays a role in the evolution of skin necrosis.
authors.affiliation: Department of Pathology, University of Maryland, Baltimore 21201.
authors.forename: L
authors.initials: L
authors.lastname: Marzella
authors.forename: R R
authors.initials: RR
authors.lastname: Jesudass
authors.forename: P N
authors.initials: PN
authors.lastname: Manson
authors.forename: R A
authors.initials: RA
authors.lastname: Myers
authors.forename: G B
authors.initials: GB
authors.lastname: Bulkley
mesh_terms: D000009:Abdominal Muscles; D000818:Animals; D002199:Capillary Permeability; D004730:Endothelium, Vascular / Q000648:ultrastructure; D005452:Fluoresceins; D006801:Humans; D007511:Ischemia / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D011868:Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply* / Q000473:pathology / Q000648:ultrastructure; D013482:Superoxide Dismutase / Q000494:pharmacology; D013524:Surgical Flaps
chemical_list: D005452:Fluoresceins; D011868:Radioisotopes; D013482:Superoxide Dismutase