title: Chitin-coated Celite as an affinity adsorbent for high-performance liquid chromatography of lysozyme.
abstract: Preparation of chitin-coated Celite as an affinity adsorbent for high-performance liquid chromatography of lysozymes and its application to separation of N-bromosuccinimide-oxidized lysozymes are described. By pH gradient elution, two diastereomers of oxindolealanine-62-lysozyme, delta 1-acetoxytryptophan-62-lysozyme (intermediate product in the reaction in acetate buffer), and native lysozyme were all separated within 40 min.
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Fukumura
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Imoto
mesh_terms: D000818:Animals; D002645:Chickens; D002686:Chitin; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007692:Diatomaceous Earth; D004529:Egg White; D009113:Muramidase / Q000302:isolation & purification*; D012822:Silicon Dioxide
chemical_list: D002686:Chitin; D007692:Diatomaceous Earth; D012822:Silicon Dioxide; D009113:Muramidase