Immunoregulation and prostaglandin production by mechanically-derived and enzyme-derived murine decidual cells.
Uterine cells from pregnant mice release soluble mediators which suppress in vitro immune responses non-specifically. Two methodologic approaches have been employed to obtain regulatory cells, and, although all cell populations derived by both methods were immunosuppressive, different factors and possibly different mechanisms of suppression appear to have been involved. The current study was designed to determine how suppression by mechanically and enzymatically derived cells might differ. Both enzymatically and mechanically derived cell populations suppressed mixed lymphocyte reactions non-specifically, mediated their effects through release of soluble factors, were immunosuppressive whether added at initiation or near the end of culture, suppressed generation of both IL-1 and IL-2, and suppression was not reversible with IL-1, IL-2, gamma interferon or combinations of the above. Immunosuppression by enzymatically derived, but not mechanically derived cells, was reversible with indomethacin. Enzymatically derived cells, but not mechanically derived cells, produced considerable amounts of PGE2. Morphological and immunohistochemical analysis revealed significant numbers of macrophages in both populations. Enzymatic treatment of mechanically derived cells did not increase the output of PGE2 when cells were cultured in vitro. The results suggest that, despite several similarities, enzymatically and mechanically derived cell populations are distinct and produce a distinct profile of soluble suppressive molecules.
Wood
G W
GW
Department of Pathology and Oncology, University of Kansas Medical Center, Kansas City 66103.
Kamel
S
S
Smith
K
K
D000818:Animals; D002469:Cell Separation; D003656:Decidua / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D007108:Immune Tolerance; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D008222:Lymphokines / Q000096:biosynthesis; D008431:Maternal-Fetal Exchange; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D011453:Prostaglandins / Q000096:biosynthesis*; D013491:Suppressor Factors, Immunologic / Q000302:isolation & purification
D008222:Lymphokines; D011453:Prostaglandins; D013491:Suppressor Factors, Immunologic; D015232:Dinoprostone