[The occurrence of Fusarium varieties and their mycotoxins on silo corn. 1. A method for the determination of zearalenone in corn and corn silage by high performance liquid chromatography (HPLC) with fluorescence detection].
A method for the determination of zearalenone in maize and maize silage was developed which distinguishes itself by the effective and fast cleaning of the extracts with the help of a silica gel minicolumn. The samples were extracted with chloroform/methanol (9 + 1) and cleaned on a silica gel minicolumn after acid-base partition. The zearalenone was quantitatively determined optionally by means of high-performance liquid chromatography (HPLC) with fluorescence detection (excitation wavelength 236 nm, emission filter 418 nm) or thin-layer chromatography (TLC), p-methoxybenzene diazonium fluoroborate and aluminium chloride were used as detection chemicals. The limits of detection are 0.01 mg/kg (HPLC) and 0.1 mg/kg resp. (TLC), the average recovery is 81%. The method was used for the determination of zearalenone in grain maize, CCM silage and silage from whole maize plants.
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Institut für Futterproduktion Paulinenaue, Akademie der Landwirtschaftswissenschaften der DDR.
D000821:Animal Feed / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005670:Fusarium; D009183:Mycotoxins / Q000032:analysis*; D012118:Resorcinols / Q000032:analysis*; D012820:Silage / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D003313:Zea mays / Q000032:analysis*; D015025:Zearalenone / Q000032:analysis*
D009183:Mycotoxins; D012118:Resorcinols; D015025:Zearalenone
Vorkommen von Fusarium-Arten und ihren Mykotoxinen auf Silomais. 1. Mitteilung. Methode zur Bestimmung von Zearalenon in Mais und Maissilage durch Hochleistungs-Flüssigkeitschromatographie (HPLC) mit Fluoreszenzdetektion.