New 5'-nucleotidase inhibitors, melanocidin A and melanocidin B. I. Taxonomy, fermentation, isolation and biological properties.
New 5'-nucleotidase inhibitors named melanocidins A and B were produced by Nocardioides sp. 1681 J. They were isolated from fermentation broth by trichloracetic acid extraction, ethanol precipitation and CM-cellulose and DEAE-cellulose column chromatography. They inhibited 5'-nucleotidase activity from snake venom but not from rat liver membrane at 200 micrograms/ml. They also show antitumor activity against melanoma B16.
Ogawara
H
H
Uchino
K
K
Akiyama
T
T
Watanabe
S
S
D015720:5'-Nucleotidase; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D005285:Fermentation; D009708:Nucleotidases / Q000037:antagonists & inhibitors*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D013306:Streptomycetaceae / Q000378:metabolism
D000970:Antineoplastic Agents; D011134:Polysaccharides; C046077:melanocidin A; C046078:melanocidin B; D009708:Nucleotidases; D015720:5'-Nucleotidase