Influence of cholesterol oxidation derivatives on membrane bound enzymes in cultured aortic smooth muscle cells.
Cholestane-3 beta, 5 alpha, 6 beta-triol and 25-hydroxycholesterol are two of the most cytotoxic and also relatively abundant of the autoxidation derivatives of cholesterol. Cultured aortic smooth muscle cells which were incubated with 10 micrograms/ml of either sterol for 24 to 48 hr showed a marked decrease of 5'-nucleotidase activity in isolated crude membranes. It was further demonstrated that 5'-nucleotidase activity was also markedly decreased in plasma membrane-enriched fractions when cells were incubated with cholestane-3 beta,5 alpha,6 beta-triol. Na+,K+-ATPase activity in crude membranes showed a significant decrease (32%) only in cells incubated with cholestane-3 beta,5 alpha,6 beta-triol for 48 hr. There was no effect on Na+,K+-ATPase activity in cells incubated for 24 hr with either sterol.
Peng
S K
SK
Hill
J C
JC
Morin
R J
RJ
Taylor
C B
CB
D015720:5'-Nucleotidase; D000818:Animals; D001011:Aorta / Q000201:enzymology; D002462:Cell Membrane / Q000201:enzymology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002777:Cholestanols / Q000494:pharmacology*; D006888:Hydroxycholesterols / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D009708:Nucleotidases / Q000378:metabolism*; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D002777:Cholestanols; D006888:Hydroxycholesterols; D000960:Hypolipidemic Agents; C000474:cholestane-3,5,6-triol; C007997:25-hydroxycholesterol; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000254:Sodium-Potassium-Exchanging ATPase