Effect of prostaglandin E2 on PZ-peptidase and several other peptidase activities in a clonal osteoblast-like cell line derived from newborn mouse calvaria.
The effects of prostaglandin E2(PGE2) on the degradation of collagen and non-collagenous peptides in clonal osteoblastic MC3T3-E1 cells were investigated by using highly sensitive assay methods for PZ-peptidase, collagenase-like peptidase (CL-peptidase), dipeptidyl-aminopeptidase (DAP), leucine aminopeptidase (LAP), and post-proline cleaving enzyme (PPCE). PGE2, at concentrations of 0.1 to 4.0 micrograms/ml, doubled the PZ-peptidase and CL-peptidase activities in the cells on 24 h culturing in a dose-dependent manner. PGE2, at a concentration of 2.0 micrograms/ml, enhanced the specific activities of PZ-peptidase, CL-peptidase, DAP, LAP, and PPCE for 75 h after the start of PGE2 stimulation. The time dependent changes in PZ-peptidase and CL-peptidase activities showed similar patterns, and 3- and 2-fold increases were seen after 48 h, respectively. The protein and DNA contents gradually increased after addition of PGE2. Since the PZ-peptidase and CL-peptidase, involved in degradation of collagen peptides, were significantly induced by PGE2 in comparison with LAP and PPCE, involved in the degradation of non-collagenous peptides, these results show that PGE2 specifically stimulates induction of collagen catabolizing enzymes in clonal osteoblasts.
Chikuma
T
T
Ishii
Y
Y
Kato
T
T
Kodama
H
H
Hakeda
Y
Y
Kumegawa
M
M
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells; D004247:DNA / Q000096:biosynthesis; D015232:Dinoprostone; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008666:Metalloendopeptidases; D051379:Mice; D008810:Mice, Inbred C57BL; D003012:Microbial Collagenase / Q000378:metabolism; D010006:Osteoblasts / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D000085822:Prolyl Oligopeptidases; D011458:Prostaglandins E / Q000494:pharmacology*; D012697:Serine Endopeptidases
D011458:Prostaglandins E; D004247:DNA; D010450:Endopeptidases; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase; D003012:Microbial Collagenase; D015232:Dinoprostone