Mismatched dsRNA (ampligen) induces protection against genomic variants of the human immunodeficiency virus type 1 (HIV-1) in a multiplicity of target cells.
Mismatched double-stranded RNA of the form r(I)n.r(C12-U)n (Ampligen) has been shown to be active against human immunodeficiency virus type 1 (HIV-1) using CEM and C3 cells as targets for infection by the highly similar HIV-1 isolates HTLV-IIIB and LAV (Montefiori, D.C. and Mitchell, W.M., 1987, Proc. Natl. Acad. Sci. U.S.A., 84, 2985-2989). The scope of Ampligen's anti-HIV-1 activity was examined in this study using the genetically divergent HIV-1 isolate HTLV-IIIRF, two additional target T-cell lines, H9 and MT-2, and a monocyte/macrophage cell line, U937. As judged by indirect immunofluorescence, reverse transcriptase activity and vital dye uptake, Ampligen was active against HTLV-IIIRF in H9, MT-2, C3 and U937 cells in addition to being active against HTLV-IIIB in U937 cells. A minimum of 1 h preincubation of cells (MT-2) with Ampligen was required for maximum activity. These results suggest that Ampligen's potential clinical efficacy may not be limited by either the highly variable nature or host cell range of HIV-1.
Montefiori
D C
DC
Department of Pathology, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232.
Robinson
W E
WE
Mitchell
W M
WM
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D014644:Genetic Variation; D006678:HIV / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008264:Macrophages; D009000:Monocytes; D011070:Poly I-C; D011072:Poly U; D011131:Polyribonucleotides / Q000494:pharmacology*; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D006377:T-Lymphocytes, Helper-Inducer
D000998:Antiviral Agents; D011131:Polyribonucleotides; D012330:RNA, Double-Stranded; D011072:Poly U; C047490:poly(I).poly(c12,U); D011070:Poly I-C