Phosphorylation of neurofilament proteins by endogenous calcium/calmodulin-dependent protein kinase.
A protein fraction containing neurofilaments was prepared from rat brain cytosol by differential centrifugation and gel filtration chromatography. These preparations were enriched for a calcium/calmodulin-dependent kinase activity that phosphorylated endogenous neurofilament proteins. The enzyme incorporated approximately 1 mol PO4/mol of each neurofilament triplet polypeptide. These data suggest that a calmodulin-dependent kinase may mediate some of the effects of calcium on cytoskeletal function by phosphorylation of neurofilament proteins.
Vallano
M L
ML
Buckholz
T M
TM
DeLorenzo
R J
RJ
D000818:Animals; D001921:Brain / Q000201:enzymology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002147:Calmodulin / Q000302:isolation & purification; D002417:Cattle; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007381:Intermediate Filament Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D016900:Neurofilament Proteins; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism
D002147:Calmodulin; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases