Heparan sulfate proteoglycan from human and equine glomeruli and tubules.
1. Proteoglycans were isolated from human and equine glomeruli or tubules by guanidine extraction and anion exchange chromatography. 2. These proteoglycan preparations contained about equal amounts of heparan sulfate and chondroitin sulfates. 3. During the preparation of glomerular or tubular basement membranes the main part of proteoglycans (greater than 50%) was extracted in the salt extract. Chondroitin sulfate proteoglycan was mainly found in the water and salt extracts of glomeruli and tubules, heparan sulfate proteoglycan in the deoxycholate extracts and the basement membranes. 4. The glomerular basement membrane (GBM) contains about 12% (human) or 20% (equine) of the proteoglycans of the total glomerulus. They consist of greater than 70% (equine) or 80% (human) of heparan sulfate. 5. Heparan sulfate proteoglycan was isolated from the proteoglycan preparations of human or equine glomeruli and tubules by additional treatment with nucleases and chondroitinase ABC followed by CsCl gradient centrifugation. 6. Protein accounts for about 40% (dry weight) of the heparan sulfate proteoglycans. Their amino acid composition is characterized by a high content of glycine, but 3-hydroxyproline, 4-hydroxyproline and hydroxylysine are lacking. 7. The biochemical characteristics of the heparan sulfate proteoglycan of human or equine glomeruli or tubules differ from that isolated from rat glomeruli by their higher protein content and their amino acid composition. The significance of these differences is discussed.
van den Heuvel
L P
LP
Department of Biochemistry, University of Nijmegen, The Netherlands.
Veerkamp
J H
JH
Monnens
L A
LA
Schröder
C H
CH
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001894:Borohydrides / Q000494:pharmacology; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D004588:Electrophoresis, Cellulose Acetate; D006025:Glycosaminoglycans / Q000302:isolation & purification* / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000032:analysis / Q000302:isolation & purification*; D006736:Horses; D006801:Humans; D007678:Kidney Glomerulus / Q000032:analysis*; D007684:Kidney Tubules / Q000032:analysis*; D008566:Membranes; D009608:Nitrous Acid / Q000494:pharmacology; D011509:Proteoglycans / Q000302:isolation & purification*
D000596:Amino Acids; D001894:Borohydrides; D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D009608:Nitrous Acid; D011509:Proteoglycans; C025364:sodium borohydride