Synthetic CD4 peptide derivatives that inhibit HIV infection and cytopathicity.
Synthetic peptide segments of the CD4 molecule were tested for their ability to inhibit infection of CD4+ cells by the human immunodeficiency virus (HIV) and to inhibit HIV-induced cell fusion. A peptide mixture composed of CD4(76-94), and synthesis side products, blocked HIV-induced cell fusion at a nominal concentration of 125 micromolar. Upon high-performance liquid chromatography, the antisyncytial activity of the peptide mixture was found not in the fraction containing the peptide CD4(76-94) itself, but in a side fraction containing derivatized peptide products generated in the automated synthesis. Derivatized deletion and substitution peptides in the region CD4(76-94) were used to demonstrate sequence specificity, a requirement for benzyl derivatization, and a core seven-residue fragment required for antisyncytial activity. A partially purified S-benzyl-CD4(83-94) peptide mixture inhibited HIV-induced cell fusion at a nominal concentration of less than or equal to 32 micromolar. Derivatized CD4 peptides blocked cell fusion induced by several HIV isolates and by the simian immunodeficiency virus, SIV, and blocked infection in vitro by four HIV-1 isolates with widely variant envelope gene sequences. Purified CD4(83-94) dibenzylated at cysteine 86 and glutamate 87 possessed antisyncytial activity at 125 micromolar. Derivatization may specifically alter the conformation of CD4 holoreceptor peptide fragments, increasing their antiviral efficacy.
Lifson
J D
JD
Division of Cellular Immunology, Genelabs Incorporated, Redwood City, CA 94063.
Hwang
K M
KM
Nara
P L
PL
Fraser
B
B
Padgett
M
M
Dunlop
N M
NM
Eiden
L E
LE
D000595:Amino Acid Sequence; D000945:Antigens, Differentiation, T-Lymphocyte / Q000302:isolation & purification / Q000494:pharmacology; D000998:Antiviral Agents; D015704:CD4 Antigens; D002459:Cell Fusion; D002851:Chromatography, High Pressure Liquid; D006678:HIV / Q000187:drug effects / Q000502:physiology*; D007959:Lymphocyte Culture Test, Mixed; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000998:Antiviral Agents; D015704:CD4 Antigens; D010446:Peptide Fragments; C056725:CD4 (76-94); C056724:S-benzyl-CD4 (83-94) peptide