Elimination of silica gel from gangliosides by using a reversed-phase column after preparative thin-layer chromatography.
A simple and effective procedure has been developed for eliminating silica gel from gangliosides after preparative thin-layer chromatography. Gangliosides were extracted from the scraped silica gel with chloroform-methanol-water 10:10:3 (by volume) and dried. The residue was suspended in 0.1 M NaCl and centrifuged. After filtration through a sintered glass funnel, the supernatant was applied to a reversed-phase column. The column was washed with 0.1 M NaCl, water, and methanol-water 1:1 (v/v). Gangliosides were eluted from the cartridge with methanol with yields of 92% and 90% in terms of dry weight and sialic acid content, respectively. Almost all of the silica gel contamination was eliminated from gangliosides by this procedure.
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D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002845:Chromatography / Q000379:methods; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000302:isolation & purification*; D058428:Silica Gel; D012822:Silicon Dioxide / Q000302:isolation & purification*
D005732:Gangliosides; D058428:Silica Gel; D012822:Silicon Dioxide