Adenovirus type 12 E1A gene represses accumulation of MHC class I mRNAs at the level of transcription.
The levels of the class I antigens and mRNAs of the major histocompatibility complex (MHC) are greatly diminished in cells transformed by adenovirus type 12 (Ad12). Although the Ad12-transforming gene, E1A, is responsible for reduced class I expression, the site at which E1A blocks accumulation of class I transcripts is not known. In this study, we demonstrate by nuclear run-on assays that in Ad12-transformed mouse cells, E1A acts by reducing the rate of transcription of class I genes.
Friedman
D J
DJ
Wistar Institute, Philadelphia, Pennsylvania 19104.
Ricciardi
R P
RP
D000256:Adenoviridae / Q000502:physiology*; D017456:Adenovirus Early Proteins; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D005805:Genes, MHC Class I; D006183:H-2 Antigens / Q000096:biosynthesis*; D007668:Kidney; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic
D017456:Adenovirus Early Proteins; D006183:H-2 Antigens; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger