Two human genes encoding tRNA(GCCGly).
Two human DNA fragments of 16.7 and 15.5 kb have been selected from a human lambda Charon-4A library by hybridization to an unfractionated tRNA probe. Restriction mapping and Southern and Northern hybridization analyses revealed the presence of a single tRNA-hybridizing region in each of the human DNA fragments. Nucleotide sequence analysis has identified two identical members of the tRNA(GCCGly) gene family. These tRNA(GCCGly) genes encode all of the conserved and semiconserved nucleotides of the tDNA split promoter sequences. Neither gene contains introns or encodes the CCA sequence present on the 3' terminus of mature tRNA. One of these identical tRNA(GCCGly) genes was found to be expressed at a substantially greater efficiency than the other in a HeLa cell lysate in vitro transcription system. No similarity was detected in the nucleotide sequences flanking these genes other than the characteristic, 3' oligo[dT] transcription termination signals and a TCTTT sequence located 7 to 10 bp upstream. These data are consistent with the hypothesis that as yet unidentified tDNA flanking sequences may have an important role in modulating human tRNA gene expression.
Doran
J L
JL
Department of Microbiology, University of Alberta, Edmonton, Canada.
Bingle
W H
WH
Roy
K L
KL
D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D004274:DNA, Recombinant; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012353:RNA, Transfer, Gly / Q000235:genetics*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012345:RNA, Transfer, Amino Acid-Specific; D012353:RNA, Transfer, Gly