Inhibitory effect of GABA on sympathetic neurotransmission in rabbit ear artery.
GABA 100 microM inhibited neurogenic vasoconstrictor responses elicited in rabbit ear artery by field stimulation at various frequencies. GABA was ineffective both on resting tone and on noradrenaline (0.05-5 microM)- and high-K+ (24-54 mM)-induced tonic contraction. GABA effectiveness against field stimulation-induced vasoconstriction was inversely related to the frequency of stimulation. This action of GABA was mimicked by a selective GABAB agonist, such as baclofen (100 microM), but not by selective GABAA agonists as muscimol (100 microM) and homotaurine (100 microM). The selective GABAB antagonists 5-aminovaleric acid (1 mM) and homotaurine (100 microM) completely suppressed the inhibitory effect of GABA on field stimulation-induced vasoconstrictions. GABA action was partially reversed also by the selective GABAA antagonist picrotoxin (100 microM); however, this drug "per se" determined an increase in amplitude of field stimulation-induced contractions which in turn could have determined the minor effect of GABA. As a whole these data suggest that GABA can inhibit sympathetic neurotransmission in rabbit ear artery through the stimulation of a prejunctional receptor of the GABAB subtype.
Manzini
S
S
Maggi
C A
CA
Meli
A
A
D000596:Amino Acids / Q000494:pharmacology; D021542:Amino Acids, Neutral; D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000294:innervation; D001418:Baclofen / Q000494:pharmacology; D004431:Ear, External / Q000098:blood supply; D004558:Electric Stimulation; D018756:GABA Antagonists; D066298:In Vitro Techniques; D008297:Male; D009118:Muscimol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010852:Picrotoxin / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011817:Rabbits; D013564:Sympathetic Nervous System / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects*; D013654:Taurine / Q000031:analogs & derivatives / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D000596:Amino Acids; D021542:Amino Acids, Neutral; D001418:Baclofen; D018756:GABA Antagonists; D009118:Muscimol; D009638:Norepinephrine; D010852:Picrotoxin; D011188:Potassium; D013654:Taurine; D005680:gamma-Aminobutyric Acid; C001355:tramiprosate; C013809:5-aminovaleric acid