Further evidence for the presence of specific binding sites for prolactin in the rabbit brain. Preferential distribution in the hypothalamus and substantia nigra.
In the present research we have extended our work on the presence of binding sites for prolactin in the rabbit brain focusing our attention on the brain areas with high dopamine cell bodies density. Among these areas the hypothalamus showed the highest specific binding of labeled ovine prolactin (oPRL). Clearly detectible specific binding was observed also in substantia nigra, whereas in other brain regions the specific binding was very small, except for the striatum where a low but not negligible binding was found in female rabbits. The binding of 125I-oPRL showed a hormonal specificity and Scatchard analysis of the binding showed no clear difference in dissociation constant (Kd) between hypothalamus, nigra and striatum.
Di Carlo
R
R
Muccioli
G
G
Lando
D
D
Bellussi
G
G
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D004298:Dopamine / Q000502:physiology; D005260:Female; D007031:Hypothalamus / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D011388:Prolactin / Q000378:metabolism*; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011981:Receptors, Prolactin; D013378:Substantia Nigra / Q000378:metabolism*
D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D011388:Prolactin; D004298:Dopamine