Crystallization and preliminary x-ray investigation of the regulatory subunit of cAMP-dependent protein kinase.
Crystals of type I cAMP-dependent protein kinase regulatory subunit have been grown from solutions of ammonium sulfate. The crystals are square bipyramids, space group P4(1)2(1)2 (P4(3)2(1)2), with a = b = 106.9 +/- 0.6 A and c = 212.4 +/- 1.0 A. There are two dimers of the regulatory subunit/crystallographic asymmetric unit. The crystals are stable for 3-4 days in the x-ray beam and diffract to at least 3.5-A resolution.
Lee
J H
JH
Bechtel
P J
PJ
Phillips
G N
GN
D000818:Animals; D002417:Cattle; D003460:Crystallization; D000242:Cyclic AMP / Q000378:metabolism; D009132:Muscles / Q000201:enzymology; D011487:Protein Conformation; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D014961:X-Ray Diffraction / Q000379:methods
D000242:Cyclic AMP; D011494:Protein Kinases