title: Isolation of Fasciola hepatica metacercariae by density gradients.
abstract: Fasciola hepatica metacercariae were purified in high yield, removing contaminating cyst walls and plant material by step gradients consisting of 10 ml of 60% Percoll (density = 1.08 g ml-1) and 10 ml of 50% Metrizamide (density = 1.25 g ml-1). Greater than 90% of the metacercariae applied to the density gradients were recovered. These isolated metacercariae had an in vitro excystment rate of greater than 80%, which was the same excystment rate as metacercariae not subjected to density gradient centrifugation.
authors.forename: T R
authors.initials: TR
authors.lastname: Fry
authors.forename: J J
authors.initials: JJ
authors.lastname: Reddington
authors.forename: R B
authors.initials: RB
authors.lastname: Wescott
authors.forename: R W
authors.initials: RW
authors.lastname: Leid
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D005210:Fasciola hepatica / Q000254:growth & development; D008793:Metrizamide; D010274:Parasitology / Q000379:methods*; D011205:Povidone; D012822:Silicon Dioxide
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; D008793:Metrizamide