Plasmid fusions mediated by one end of TnA.
We have observed plasmid fusions in a recA background mediated by a single end of TnA. These occur when transposase is provided either in cis or in trans. Insertions of the plasmid carrying the TnA inverted repeat sequence occur at many sites in the target plasmid. The point of fusion on the plasmid carrying TnA sequences always appears to be located in the region which carries the TnA inverted repeat sequence. In contrast to the transposition of an intact TnA element, plasmid fusions mediated by one end of TnA are very rare events. The implications of our results for models of transposition are discussed.
Heritage
J
J
Bennett
P M
PM
D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005819:Genetic Markers; D010957:Plasmids; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D005819:Genetic Markers; D004262:DNA Restriction Enzymes