Modulation of lymphocyte adrenergic receptors by hormones and anti-asthmatic drugs.
Viable lymphocytes were used to investigate the binding of physiological concentrations of hormones and therapeutic concentrations of anti-asthmatic drugs to [3H]dihydroalprenolol (DHA, beta antagonist) and [3H]prazosin (alpha antagonist) receptor sites. All studied drugs and hormones inhibited the specific binding of prazosin to receptors identified with 10(-4)M phentolamine. Similar concentrations of drugs and hormones, with the exception of prostaglandin F2 alpha and cimetidine, inhibited the specific binding of DHA to lymphocyte receptors identified with 10(-7)M propranolol. These results confirm current mechanisms of hormone and drug action in asthma.
Williams
W R
WR
Davies
B H
BH
D001249:Asthma / Q000188:drug therapy; D001667:Binding, Competitive; D004082:Dihydroalprenolol / Q000378:metabolism; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D011224:Prazosin / Q000378:metabolism; D011433:Propranolol / Q000378:metabolism; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*
D006728:Hormones; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D011433:Propranolol; D011224:Prazosin