In vivo and in vitro autoradiographic labelling of central dopaminergic systems with [3H]GBR12783 in rodents.
Labelling of central dopaminergic systems with the dopamine uptake inhibitor [3H]GBR12783 was performed in vivo in mice by i.v. injection of a tracer dose of the ligand (40 microCi) 1 h before sacrifice. Autoradiographic pictures obtained from these experiments were quantified. The data were compared to those obtained by in vitro labelling of rat brain slices with this ligand. Significant specific labelling was observed both in vivo and in vitro in the striatum, the nucleus accumbens, the olfactory tubercle and the substantia nigra.
Leroux-Nicollet
I
I
Unité de Neuropsychopharmacologie Expérimentale, U.A. CNRS 1170, Saint-Etienne-du-Rouvray, France.
Costentin
J
J
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D014179:Neurotransmitter Uptake Inhibitors / Q000378:metabolism*; D010879:Piperazines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D014179:Neurotransmitter Uptake Inhibitors; D010879:Piperazines; C039737:1-(2-(diphenylmethoxy)ethyl)-4-(3-phenyl-2-propenyl)piperazine; D004298:Dopamine