Coexpression of glial fibrillary acidic protein and vimentin in the central and peripheral nervous systems of the twitcher mutant.
A method to purify glial fibrillary acidic protein (GFAP) from mouse spinal cord is described, which permits the measurement of GFAP in the sciatic nerve of the twitcher mutant and control mouse. Cytoskeletal proteins from sciatic nerves and purified GFAP standards were electrophoresed on gel, transferred to nitrocellulose paper, and immunostained with anti-GFAP antibody. From the immunostained, 51,000-dalton band, we estimated about 200 ng GFAP per 50 micrograms of cytoskeletal protein in the twitcher sciatic nerve. The control nerve showed no detectable GFAP. Double-labeled fluorescence immunocytochemistry showed that in the brainstem of twitcher mutant, GFAP and vimentin were coexpressed in the majority of astrocytes.
Chiu
F C
FC
Saul Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 10461.
Sacchi
R S
RS
Claudio
L
L
Kobayashi
S
S
Suzuki
K
K
D000818:Animals; D001933:Brain Stem / Q000032:analysis*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis* / Q000302:isolation & purification; D007150:Immunohistochemistry; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000378:metabolism*; D008970:Molecular Weight; D012584:Sciatic Nerve / Q000032:analysis*; D013347:Subcellular Fractions / Q000032:analysis; D014746:Vimentin / Q000032:analysis*
D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin