Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts.
Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.
Nielsen
P E
PE
Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
Jeppesen
C
C
Buchardt
O
O
D010582:Bacteriophage lambda / Q000032:analysis; D001483:Base Sequence; D004247:DNA / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004278:DNA, Superhelical / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D009875:Operator Regions, Genetic; D009942:Organometallic Compounds / Q000494:pharmacology*; D010777:Photochemistry; D010957:Plasmids; D011506:Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D014466:Ultraviolet Rays; D014501:Uranium / Q000494:pharmacology*; D014502:Uranyl Nitrate / Q000494:pharmacology*
D004278:DNA, Superhelical; D009942:Organometallic Compounds; D011506:Proteins; D012097:Repressor Proteins; D014502:Uranyl Nitrate; C005460:uranyl acetate; D014501:Uranium; D004247:DNA