Pathogenesis and virus content of lymphomas induced by pure ecotropic Graffi murine leukemia virus.
Tumors induced by wild type Graffi murine leukemia virus (Gi-MuLV) contained high titers of MuLV consisting of a predominant ecotropic (e)-MuLV and a scarcer titer recombinant (RM) MuLV component. Each of these was purified by biological cloning and examined for its envelope properties and leukemogenicity. Both the e- and the RM-MuLV's were single isolates and unique in terms of their neutralization profiles and peptide maps. The cloned e-Gi-MuLV was highly leukemogenic in C57Bl mice, inducing a very rapid lethal thymic lymphoma but no myeloid leukemia. e-Gi-MuLV also accelerated thymic lymphoma in AKR mice. The purified RM-MuLV did not induce any tumors. Infectious cell center (ICC) experiments of organs of mice inoculated with e-Gi-MuLV showed that virus replicated very rapidly and reached maximal titers in about one week in C57Bl mice. There was a highly preferential replication in the thymus of the animal so that this e-Gi-MuLV can be considered as thymotropic. Within two weeks after infection of mice, infected cells of the thymus also began to release low levels of a non-ecotropic MuLV. The rapid induction of lymphoma is compared to that induced by other e-MuLV's and their RM-MuLV's, and to the natural AKR-MuLV-associated disease. These findings are discussed in the context of prevailing theories on envelope gene rearrangements in the virus and in the proviral sequences in resulting tumors.
Fischinger
P J
PJ
Dunlop
N M
NM
Robey
W G
WG
D000818:Animals; D006023:Glycoproteins / Q000032:analysis; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D008223:Lymphoma / Q000209:etiology*; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic; D013950:Thymus Gland / Q000382:microbiology; D014759:Viral Envelope Proteins / Q000032:analysis
D006023:Glycoproteins; D014759:Viral Envelope Proteins