Activated human B cells display a functional IL 2 receptor.
These studies deal with the expression of a functional IL 2 receptor on activated primary human B cells. Antibody against the receptor (alpha-TAC) reacted with 25 to 65% activated B cells, inhibited B cell proliferation by 50% and inhibited B cell secretion of Ig by greater than 90%. These effects were shown to be independent of contaminating T lymphocytes. Anti-TAC immunoprecipitated a molecule of identical size (65,000 daltons) from T and B lymphocytes; B cells were also shown to actively synthesize the IL 2 receptor. The chymotryptic peptide chromatograms of TAC antigen from T and B cells show these molecules to be indistinguishable.
Mittler
R
R
Rao
P
P
Olini
G
G
Westberg
E
E
Newman
W
W
Hoffmann
M
M
Goldstein
G
G
D000911:Antibodies, Monoclonal / Q000502:physiology; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D001667:Binding, Competitive; D002918:Chymotrypsin / Q000494:pharmacology; D003165:Complement System Proteins / Q000502:physiology; D006462:Hemolytic Plaque Technique; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation; D010446:Peptide Fragments / Q000302:isolation & purification; D011233:Precipitin Tests; D011971:Receptors, Immunologic / Q000032:analysis*; D015375:Receptors, Interleukin-2; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007376:Interleukin-2; D010446:Peptide Fragments; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D003165:Complement System Proteins; D002918:Chymotrypsin