[Comparative effects of prostaglandins and ACTH on the production of corticosterone and cyclic AMP in isolated adrenal gland cells of rats].
The authors compared the effect of prostaglandin PGE2 and ACTH on corticosterone and cAMP production in isolated adrenal cells of rats. PGE2 stimulated steroidogenesis in the concentrations of 0.01-1.10 micrograms/ml and the maximum stimulation (2.5-fold) was observed in the PGE2 concentration of 0.1 micrograms/ml. ACTH stimulated steroidogenesis 12-fold as compared to PGE2. In combined addition of different PGE2 doses and the physiological ACTH dose (5 pg/ml) their steroid effects were summated. The results obtained are indicative of the fact that PGE2 is no mediator of the ACTH effect but that it is capable of potentiating this effect. Like ACTH, PGE2 raised the cAMP level in the adrenal gland. In concentrations causing a similar to ACTH increase in the cAMP level, PGE2 stimulated steroidogenesis to a lesser degree than ACTH, and in this connection a possibility of the existence of two cAMP foci in the adrenal gland is discussed. It is assumed that one of the foci is related and the other is not related with steroidogenesis, and PGE2 stimulates mainly the second focus.
Morozova
M S
MS
Razina
L G
LG
Goncharova
V N
VN
D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D003345:Corticosterone / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000096:biosynthesis*; D015237:Dinoprost; D015232:Dinoprostone; D066298:In Vitro Techniques; D008297:Male; D011458:Prostaglandins E / Q000494:pharmacology*; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology*; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical
D011458:Prostaglandins E; D011459:Prostaglandins E, Synthetic; D011460:Prostaglandins F; D011461:Prostaglandins F, Synthetic; D000324:Adrenocorticotropic Hormone; D015237:Dinoprost; D000242:Cyclic AMP; D015232:Dinoprostone; D003345:Corticosterone
Sravnenie deĭstviia prostaglandinov i AKTG na obrazovanie kortikosterona i tsAMF v izolirovannykh kletkakh nadpochechnikov krys.