title: cDNA sequence of the beta 2-subunit of human liver alcohol dehydrogenase.
abstract: A cDNA library of mRNA from a human liver expressing the beta 2-subunit of alcohol dehydrogenase was constructed in lambda gt11. One clone coding for 352 of a total of 374 amino acid residues of the beta 2-subunit was isolated. The sequence differed from that of the beta 1-subunit at one nucleotide position resulting in an Arg/His exchange at position 47 of the peptide chain, in agreement with data from protein sequence analysis [(1984) FEBS Lett. 173, 360-366].
authors.affiliation: Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Ehrig
authors.forename: J P
authors.initials: JP
authors.lastname: von Wartburg
authors.forename: B
authors.initials: B
authors.lastname: Wermuth
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014357:Trypsin
chemical_list: D003062:Codon; D010446:Peptide Fragments; D012333:RNA, Messenger; D009243:NAD; D004247:DNA; D000426:Alcohol Dehydrogenase; D014357:Trypsin