Ultramicroscopic morphometric, biochemical and histophotometric investigations of myocardial biopsies taken from Fallot patients before and after cardioplegia with cold Kirsch's solution.
Biopsies taken from the myocardium of 5 patients with Morbus Fallot and from 1 patient with ventricle septum defect were investigated with a combination of morphometric, biochemical and histophotometric techniques in order to study the cardioprotective effect of cold Kirsch's solution. At the final phase of cardioplegia the cardiomyocytes reveal the following alterations: The volume densities of mitochondria, of their degenerated areas and that of cytoplasmic vacuoles show a significant increase whereas that of myofilaments decreases. Cristae and matrix mitochondriales, however, show only moderate alterations without statistical significance. Biochemically the total ATP-concentration and creatine phosphate (CP)-concentration were more or less diminished, in most of the cases the activity of the myosin ATPase was increased, that of the creatine phosphate kinase (CPK) diminished. Compared with the biochemical estimations of the ATPase activity, its histophotometric estimations yielded corresponding results in 2 of 4 cases. In general our findings confirm the cardioprotective effect of Kirsch's solution. The combination of methods used gives more reliable results than one technique alone.
Welt
K
K
Karl Marx University, Leipzig, DDR.
Fitzl
G
G
Punkt
K
K
Punkt
J
J
Hutschenreiter
W
W
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000328:Adult; D002314:Cardioplegic Solutions / Q000494:pharmacology*; D002648:Child; D003402:Creatine Kinase / Q000378:metabolism; D005260:Female; D006324:Heart Arrest, Induced / Q000379:methods*; D006330:Heart Defects, Congenital / Q000601:surgery*; D006345:Heart Septal Defects, Ventricular / Q000601:surgery; D006801:Humans; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000648:ultrastructure*; D013771:Tetralogy of Fallot / Q000601:surgery; D014286:Trilogy of Fallot / Q000601:surgery
D002314:Cardioplegic Solutions; D003402:Creatine Kinase; D000251:Adenosine Triphosphatases