title: Fractionation of red diamond rattlesnake (Crotalus ruber ruber) venom: protease, phosphodiesterase, L-amino acid oxidase activities and effects of metal ions and inhibitors on protease activity.
abstract: Crotalus ruber ruber venom contains several different proteases, and the proteolytic activity of the crude venom is 6-15 times greater in adult than in juvenile venom. Venom samples were assayed for proteolytic, phosphodiesterase, L-amino acid oxidase and elastinase-like activities and were subjected to gel filtration on BioGel P-100. Two major size classes of proteases were resolved (mol. wt 67,000 and 20,500). EDTA, N-ethylmaleimide (N-EM) and 1,10-phenanthroline inhibited proteolytic activity of crude venom, and EDTA, Zn2+ and Cu2+ inhibited proteolytic activity of the fractionated venom.
authors.forename: S P
authors.initials: SP
authors.lastname: Mackessy
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D002850:Chromatography, Gel; D003435:Crotalid Venoms / Q000032:analysis*; D050830:L-Amino Acid Oxidase; D008670:Metals / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000032:analysis*; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D011480:Protease Inhibitors / Q000494:pharmacology*
chemical_list: D003435:Crotalid Venoms; D008670:Metals; D011480:Protease Inhibitors; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; D010727:Phosphoric Diester Hydrolases; D010447:Peptide Hydrolases