title: Physical properties of a plasmid-like DNA from Euglena gracilis.
abstract: A small circular extrachromosomal DNA of the flagellate protozoan Euglena gracilis has been characterized as having a contour length of 11.3 kb, with a consistent restriction map. The buoyant density (rho = 1.717) and melting temperature (tm = 89 degrees C) both indicate a base content of 59% G + C. The DNA is found in both wild-type cells and those lacking plastids. The copy number is estimated to be about 1000.
authors.forename: J R
authors.initials: JR
authors.lastname: Cook
authors.forename: R
authors.initials: R
authors.lastname: Roxby
mesh_terms: D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000302:isolation & purification*; D005056:Euglena gracilis / Q000032:analysis* / Q000235:genetics; D008854:Microscopy, Electron; D010957:Plasmids; D012093:Replicon
chemical_list: D004270:DNA, Circular; D004262:DNA Restriction Enzymes