Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W.
The aspA gene of Escherichia coli W which encodes aspartase was cloned into the plasmid vector pBR322. The nucleotide sequences of aspA and its flanking regions were determined. The aspA gene encodes a protein with a molecular weight of 52,224 consisted of 477 amino acid residues. The amino acid sequence of the protein predicted from the nucleotide sequence was consistent with those of the NH2- and COOH-terminal regions and also with the amino acid composition of the purified aspartase determined previously. Potential promoter and terminator sequences for aspA were also found in the determined sequence.
Takagi
J S
JS
Ida
N
N
Tokushige
M
M
Sakamoto
H
H
Shimura
Y
Y
D000595:Amino Acid Sequence; D000642:Ammonia-Lyases / Q000235:genetics*; D001220:Aspartate Ammonia-Lyase / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008040:Genetic Linkage; D011401:Promoter Regions, Genetic
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D000642:Ammonia-Lyases; D001220:Aspartate Ammonia-Lyase