Toxaphene: accumulation in the adrenal cortex and effect on ACTH-stimulated corticosteroid synthesis in the rat.
Uptake and distribution of [14C]toxaphene was studied in the adrenals of rats using whole-body autoradiography. An accumulation of radioactivity was seen in the adrenal cortex (zona fasciculata) 1-24 h after a single gavage of [14C]toxaphene (16 mg/kg b.w.). In in vitro studies toxaphene was found to inhibit ACTH-stimulated corticosterone synthesis in the cultured rat adrenocortical cells (IC50 2.8 X 10(-5) M). Moderate but significant inhibition (P less than 0.001) of ACTH-stimulated corticosterone synthesis was also observed in the adrenocortical cells isolated from rats after a prolonged exposure (5 weeks) to low levels (1.2 ppm) of toxaphene in feed. The results indicate a direct adrenotoxic effect of toxaphene.
Mohammed
A
A
Hallberg
E
E
Rydström
J
J
Slanina
P
P
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D005260:Female; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity*; D051381:Rats; D013997:Time Factors; D014112:Toxaphene / Q000378:metabolism / Q000633:toxicity*
D000305:Adrenal Cortex Hormones; D007306:Insecticides; D014112:Toxaphene; D000324:Adrenocorticotropic Hormone