The crystal structure of beta-lactamase from Staphylococcus aureus at 0.5 nm resolution.
The preparation, crystallization and low-resolution structure determination of beta-lactamase (EC 3.5.2.6, 'penicillinase') from Staphylococcus aureus is described. The enzyme crystallizes in space group I222 with 1 molecule per asymmetric unit and cell dimensions a = 5.45(1), b = 9.39(1) and c = 13.87(2) nm. The structure was determined at 0.5 nm resolution by using phases calculated from (NH4)2Pt(CN)4 and KAu(CN)2 derivatives. The mean figure of merit mean value of m, for the 1106 reflexions used was 0.70. Difference Fourier syntheses for data collected from crystals soaked in platinum D-methionine and in 6-(4-hydroxy-3,5-di-iodobenzamido)penicilloic acid revealed the likely position of the active site of the enzyme.
Moult
J
J
Sawyer
L
L
Herzberg
O
O
Jones
C L
CL
Coulson
A F
AF
Green
D W
DW
Harding
M M
MM
Ambler
R P
RP
D002241:Carbohydrates / Q000032:analysis; D003460:Crystallization; D007462:Iodobenzenes / Q000138:chemical synthesis; D010397:Penicillanic Acid / Q000138:chemical synthesis / Q000031:analogs & derivatives; D010405:Penicillinase / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000201:enzymology*; D014961:X-Ray Diffraction
D002241:Carbohydrates; D007462:Iodobenzenes; C044378:4-hydroxy-3,5-diiodophenylacetic acid; D010397:Penicillanic Acid; C044379:6-(4-hydroxy-3,5-diiodobenzamido)penicilloic acid; D010405:Penicillinase