Characterization of a mouse interferon gene locus I. Isolation of a cluster of four alpha interferon genes.
A BALB/c mouse genomic library was screened with a murine interferon alpha 2 (MuIFN-alpha 2) cDNA coding region fragment. Eight clones were isolated which contain different mouse chromosomal segments related to the MuIFN-alpha 2 probe and a 28 kilobase (kb) region of mouse genomic DNA containing four different MuIFN-alpha genes (alpha 1, alpha 4, alpha 5 and alpha 6) was identified and characterized; an intergenic 1000 nucleotide long conserved sequence was found to be associated with three of these four alpha genes, indicating that this alpha-IFN gene cluster evolved through tandem duplications. Sequence analysis revealed the absence of a polyadenylation site in the 3' untranslated region of MuIFN-alpha 1, and showed that one of the genes (alpha 4) contains an internal deletion of 5 amino acids in the coding region.
Kelley
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KA
Pitha
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D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D007370:Interferon Type I / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D012091:Repetitive Sequences, Nucleic Acid
D007370:Interferon Type I; D004262:DNA Restriction Enzymes