The effect of in vitro and in vivo toluene exposure on rat erythrocyte and synaptosome membrane integral enzymes.
The mechanism of the anaesthetic effect of toluene on the central nervous system (CNS) was studied by using rat erythrocyte and synaptosome membranes as nerve cell models both in vitro and in vivo. The activities of the membrane-bound integral enzymes acetylcholinesterase (AChE), total adenosine triphosphatase (total ATPase) and magnesium-activated adenosine triphosphatase (Mg2+-ATPase) were determined. A short-term exposure to 2000 p.p.m. of toluene had an inhibitory effect on the enzyme activities studied. The degree of inhibition in erythrocyte membranes in vitro and in vivo, and in synaptosome membranes in vitro were in good correlation. In in vivo conditions, the synaptosome-bound enzymes were, however, significantly more inhibited by toluene, which indicates that membranes in vivo are even more vulnerable to the toxic effects of organic solvents than they are as isolated membranes in vitro. However, our results show that in vitro experiments can be used to predict the toxic nerve cell membrane effects of organic solvents. Toluene caused similar enzyme inhibitions both in neural cell membranes and in erythrocyte membranes. Thus, even peripheral non-excitable cell membranes, like erythrocytes, can be used as nerve cell membrane models in studies on the mechanism of the anaesthesia caused by solvents.
Korpela
M
M
Department of Biomedical Sciences, University of Tampere, Finland.
Tähti
H
H
D000110:Acetylcholinesterase / Q000032:analysis / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002800:Cholinesterase Inhibitors; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000187:drug effects* / Q000201:enzymology; D014050:Toluene / Q000494:pharmacology*
D002800:Cholinesterase Inhibitors; D014050:Toluene; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase