Production of beta-human chorionic gonadotrophin by human squamous carcinoma cell lines.
Eight out of 9 human squamous carcinoma cell lines ectopically secrete the beta subunit of human chorionic gonadotrophin (beta-HCG) in amounts detectable by radioimmunoassay. In contrast, both normal and SV40-transformed keratinocytes show no such evidence of beta-HCG secretion. Ectopic beta-HCG is identical in its gel exclusion and high-performance liquid chromatographic properties to placental beta-HCG and, in radioimmunoassay, yields a dilution curve parallel to that of placental beta-HCG. Dibutyryl cyclic AMP, but not sodium butyrate, stimulates beta-HCG secretion by the one human cell line examined, LICR-LON-HN-5, suggesting that ectopic production by these cells is similar to trophoblastic beta-HCG secretion, and differs from most other non-trophoblastic cell lines previously examined.
Cowley
G
G
Smith
J A
JA
Ellison
M
M
Gusterson
B
B
D003994:Bucladesine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002460:Cell Line; D002472:Cell Transformation, Viral; D006063:Chorionic Gonadotropin / Q000032:analysis / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006258:Head and Neck Neoplasms / Q000378:metabolism; D006729:Hormones, Ectopic / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D011863:Radioimmunoassay; D013539:Simian virus 40; D012867:Skin / Q000166:cytology / Q000378:metabolism / Q000382:microbiology
D002087:Butyrates; D006063:Chorionic Gonadotropin; D006729:Hormones, Ectopic; D020148:Butyric Acid; D003994:Bucladesine