title: Quantitative and qualitative analysis of the Fc receptor for IgE (Fc epsilon RII) on human eosinophils.
abstract: In order to characterize the Fc receptor for IgE (Fc epsilon RII) on human eosinophils, we have compared the binding of human IgE myeloma protein to that of a monoclonal antibody (mAb BB10) directed against a common antigenic determinant of the Fc epsilon RII present on eosinophils, platelets and macrophages. Scatchard analysis of the binding to human eosinophils of the BB10 mAb revealed a linear monophasic binding curve, with a binding affinity of 1.17 x 10(7) M-1 and a number of 10(5) binding sites per cell. Biochemical analysis of the human eosinophil Fc epsilon R, performed by immunosorbent chromatography with either BB10 mAb or IgE, showed under nonreducing conditions a major component of 200 kDa. Under reducing conditions, 3 peptide fragments were obtained, with molecular masses of 45-50, 23 and 15 kDa. Finally, comparative analysis suggested that the Fc epsilon RII of human eosinophils and of a human macrophage cell line (U937) are structurally related and differ from the high-affinity Fc epsilon RI present on basophilic granulocytes.
authors.affiliation: Unité Mixte INSERM, Institut Pasteur, Lille, France.
authors.forename: T
authors.initials: T
authors.lastname: Jouault
authors.forename: M
authors.initials: M
authors.lastname: Capron
authors.forename: J M
authors.initials: JM
authors.lastname: Balloul
authors.forename: J C
authors.initials: JC
authors.lastname: Ameisen
authors.forename: A
authors.initials: A
authors.lastname: Capron
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel; D004804:Eosinophils / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000302:isolation & purification; D051379:Mice; D011961:Receptors, Fc / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D017455:Receptors, IgE
chemical_list: D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E