Hemagglutination assay of polypeptide coded by the pre-S region of hepatitis B virus DNA with monoclonal antibody: correlation of pre-S polypeptide with the receptor for polymerized human serum albumin in serums containing hepatitis B antigens.
The receptor for polymerized human as well as chimpanzee serum albumins has been identified on the 55-amino acid polypeptide coded by the pre-S region of hepatitis B virus DNA. Monoclonal antibodies were raised against a synthetic polypeptide of 19 amino acid residues representing a hydrophilic region of the pre-S amino acid sequence deduced from hepatitis B virus DNA. Sheep erythrocytes fixed with glutaraldehyde were coated with monoclonal antibody against the synthetic polypeptide to develop a hemagglutination assay for pre-S polypeptide. The pre-S polypeptide was detected in the serum containing hepatitis B surface antigen particles along with hepatitis B e antigen, with titers in parallel with those of the receptor for polymerized human serum albumin.
Okamoto
H
H
Imai
M
M
Usuda
S
S
Tanaka
E
E
Tachibana
K
K
Mishiro
S
S
Machida
A
A
Nakamura
T
T
Miyakawa
Y
Y
Mayumi
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D006386:Hemagglutination Tests; D006509:Hepatitis B / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D051379:Mice; D009694:Nucleic Acid Precursors / Q000032:analysis; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000032:analysis*; D012709:Serum Albumin / Q000378:metabolism*; D000075462:Serum Albumin, Human; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D009694:Nucleic Acid Precursors; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D012709:Serum Albumin; D014764:Viral Proteins; C032137:polyalbumin; D000075462:Serum Albumin, Human