Amiloride-sensitive Na+-H+ antiporter in Escherichia coli.
In everted vesicles of Escherichia coli, delta pH caused by H+ efflux through the Na+/H+ antiporter was measured by using a fluorescent dye. Amiloride inhibited the activity of the Na+/H+ antiporter. Kinetic studies showed that amiloride competed with Na+. The inhibition constant of 40 microM was obtained.
Mochizuki-Oda
N
N
Oosawa
F
F
D000584:Amiloride / Q000494:pharmacology*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D011719:Pyrazines / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers; D013050:Spectrometry, Fluorescence
D002352:Carrier Proteins; D011719:Pyrazines; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride