title: Generation and characterization of a temperature-sensitive mutation in the NS-1 gene of the autonomous parvovirus minute virus of mice.
abstract: In-phase single-codon insertion mutations were constructed in the open reading frames of the NS-1 and NS-2 genes of the autonomous parvovirus minute virus of mice. A viral mutant containing an isoleucine insertion exclusively within NS-1 between residues 229 and 230 was isolated that produced approximately 3 orders of magnitude fewer plaques at 39 degrees C than at 32 degrees C. Preliminary characterization of the mutant demonstrated that the NS-1 gene product is independently required for both genome amplification and the regulation of the temporal expression between the two viral transcription units during lytic infection.
authors.affiliation: Department of Microbiology, School of Medicine, University of Missouri-Columbia 65212.
authors.forename: G E
authors.initials: GE
authors.lastname: Tullis
authors.forename: L
authors.initials: L
authors.lastname: Labieniec-Pintel
authors.forename: K E
authors.initials: KE
authors.lastname: Clemens
authors.forename: D
authors.initials: D
authors.lastname: Pintel
mesh_terms: D002213:Capsid / Q000235:genetics*; D003062:Codon; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000235:genetics; D005784:Gene Amplification; D005786:Gene Expression Regulation; D005814:Genes, Viral; D008915:Minute Virus of Mice / Q000235:genetics* / Q000254:growth & development; D010321:Parvoviridae / Q000235:genetics*; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics; D013696:Temperature; D014758:Viral Core Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins; D014779:Virus Replication
chemical_list: D003062:Codon; D004279:DNA, Viral; D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins