Reversible induction of right ventricular atriopeptin synthesis in hypertrophy due to hypoxia.
Right ventricular hypertrophy produced in rats exposed to 10% oxygen for 3 weeks resulted in a ninefold increase in atriopeptin immunoreactivity (APir) and a 160-fold increase in atriopeptin messenger RNA (AP mRNA) in the right ventricular myocardium. A small but significant increase in left ventricular APir and AP mRNA was also present, probably representing the interventricular septum. Right atrial APir was decreased by 50%, but left atrial APir was not different from normoxic controls. Purification of ventricular tissue extracts by high-performance liquid chromatography revealed primarily the high molecular weight prohormone. The development of right ventricular hypertrophy and right ventricular APir content followed a similar time course, each evident at 7 days of hypoxia and reaching a plateau at 14 days. Hypoxia followed by normoxia caused right ventricular APir to fall to control levels within 3 days, despite persistent right ventricular hypertrophy. This data demonstrates that hypoxia can reversibly induce extra-atrial expression of atriopeptin synthesis in the cardiac ventricle.
Stockmann
P T
PT
Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110.
Will
D H
DH
Sides
S D
SD
Brunnert
S R
SR
Wilner
G D
GD
Leahy
K M
KM
Wiegand
R C
RC
Needleman
P
P
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis*; D006332:Cardiomegaly / Q000209:etiology / Q000378:metabolism*; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology; D000860:Hypoxia / Q000150:complications*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor