Gonadotrophin- and androgen precursor-stimulated testosterone secretion by interstitial cells from Mongolian gerbil testes: influence of plasma proteins and elevated temperature.
Interstitial cells isolated from Mongolian gerbil testes have been used to investigate the effects of plasma proteins and incubation temperature on HCG- and androgen precursor-stimulated testosterone secretion. Short term (15 min) incubation of interstitial cells with various precursors resulted in a significant increase of testosterone release. On the other hand, no stimulatory effect of HCG (10 mIU) could be observed. Precursor (e.g. progesterone)-stimulated testosterone secretion linearly increased with cell concentrations (0.5 x 10(5) to 4.0 x 10(5) cells/0.7 ml medium, r = less than 0.99, p less than 0.001). In the presence of 50% horse plasma, progesterone-stimulated testosterone secretion was even more pronounced. Similarly, also gerbil, rat, calf or human plasma significantly increased progesterone-stimulated testosterone output. Interestingly, this effect was markedly reduced in the presence of cortisol. While incubation of interstitial cells for 15 min at either 40 degrees C or 42 degrees C had no significant effect on androgen precursor- or HCG-stimulated testosterone secretion, incubation of cells at 44 degrees C resulted in a drastic reduction of HCG-stimulated testosterone release, without affecting progesterone- or DHEA-stimulated testosterone secretion. Taken the simplicity to make interstitial cells unresponsive to HCG into account, heat-treated cells might prove to be a versatile tool to distinguish between HCG- and protein-/androgen precursor-stimulated testosterone secretion in vitro.
Fenske
M
M
Department of Animal Physiology, University of Bayreuth, F.R.G.
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D005849:Gerbillinae / Q000502:physiology*; D006358:Hot Temperature; D007985:Leydig Cells / Q000187:drug effects / Q000502:physiology*; D008297:Male; D011374:Progesterone / Q000494:pharmacology; D011498:Protein Precursors / Q000494:pharmacology; D013739:Testosterone / Q000378:metabolism*
D000728:Androgens; D001798:Blood Proteins; D006063:Chorionic Gonadotropin; D011498:Protein Precursors; D013739:Testosterone; D003687:Dehydroepiandrosterone; D011374:Progesterone