title: MgATP-dependent, glucose 6-phosphate-stimulated liver microsomal Ca2+ accumulation: difference between rough and smooth microsomes.
abstract: Some features of the MgATP-dependent Ca2+-accumulating capacity of rough as compared to smooth liver microsomal fraction were studied. Smooth microsomes accumulate somewhat higher amounts of Ca2+ than rough ones in the presence of MgATP. In the presence of glucose 6-phosphate, which markedly stimulates MgATP-dependent Ca2+ accumulation in both fractions, smooth microsomes exhibit a much higher Ca2+-accumulating capacity than rough ones. Possible reasons of the differences observed between the two fractions were investigated. Smooth microsomes exhibit a higher Ca2+-dependent ATPase activity, suggesting a higher Ca2+ inward transport into smooth vesicles. Also, following the inhibition of active Ca2+ transport by means of vanadate, smooth microsomes appear to release the Ca2+ previously accumulated--both in the absence (i.e., with MgATP only) and in the presence of glucose 6-phosphate--at a lower rate than rough ones. This indicates a lower passive backflux of Ca2+ accumulated in smooth vesicles. On the basis of these data, differences can be envisaged with respect to cellular Ca2+ handling by different domains of endoplasmic reticulum in the liver.
authors.affiliation: Istituto di Patologia Generale, Università di Siena, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Romani
authors.forename: R
authors.initials: R
authors.lastname: Fulceri
authors.forename: A
authors.initials: A
authors.lastname: Pompella
authors.forename: A
authors.initials: A
authors.lastname: Benedetti
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002451:Cell Compartmentation / Q000187:drug effects; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D005958:Glucosephosphates / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D014638:Vanadates / Q000494:pharmacology
chemical_list: D005958:Glucosephosphates; D000001:Calcimycin; D014638:Vanadates; D000255:Adenosine Triphosphate; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium