Elevated growth factor levels in transformed mouse embryo cells treated with N,N-dimethylformamide.
The transformed mouse embryo fibroblast cell line AKR-MCA, produces several transforming growth factor (TGF) activities which can be identified in cell extracts and serum-free conditioned medium. Treatment of these transformed cells with 1% N,N-dimethylformamide resulted in a more normal phenotype and an increased level of TGFs in cell extracts and conditioned medium. In addition, an 11-fold increase in an epidermal growth factor receptor-competing activity was observed in cell extracts and conditioned medium compared to control untreated cells. Fractionation of the conditioned medium on Bio-Gel P-100 showed that the same size classes of TGFs were present in N,N-dimethylformamide-treated as well as untreated cells. The increased EGF-receptor-competing activity was eluted in two peaks at Mr 6500 and Mr 4000. The Mr 6500 peak did not coelute with a TGF peak in the N,N-dimethylformamide-treated cells.
Levine
A E
AE
Hamilton
D A
DA
McRae
L J
LJ
Brattain
M G
MG
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002850:Chromatography, Gel; D004126:Dimethylformamide / Q000494:pharmacology*; D004622:Embryo, Mammalian; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors; D005260:Female; D006133:Growth Substances / Q000096:biosynthesis*; D006801:Humans; D051379:Mice; D008806:Mice, Inbred AKR; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000378:metabolism; D015291:Transforming Growth Factors
D006133:Growth Substances; D010455:Peptides; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D004126:Dimethylformamide; D066246:ErbB Receptors