Proliferative and/or cytotoxic activity of lymphocyte clones to autologous human melanoma.
Peripheral blood lymphocytes (PBL) of a patient with metastatic melanoma were cultured with autologous melanoma cells (Auto-Me) and recombinant interleukin 2 (IL-2) (MLTC-PBL). Thirty-five days later, when no cytotoxicity against Auto-Me or K562 was detectable, MLTC-PBL were cloned in the presence of Auto-Me, IL-2 (25 U/ml) and Daudi cells as feeder. Eighty-one growing clones were simultaneously screened for proliferative and cytotoxic activity to Auto-Me. Twenty-two clones proliferated in the presence of Auto-Me only, 29 in the presence of IL-2 only and 41 in the presence of Auto-Me plus IL-2; 12 clones showed cytotoxic activity against Auto-Me. Six clones expressed both cytotoxic and proliferative activity to Auto-Me. The phenotype of 6 proliferative clones tested was CD3+, CD4+, WT31+, CD8-, CD16-, Leu19-, whereas that of 2 cytotoxic-proliferative clones tested was CD3+, CD8+, Leu19+, WT31+, CD4-, CD16-. Specificity analysis of proliferative response of 6 clones and of cytotoxicity of 7 clones, tested on a panel of 14 different target cells, revealed a complex pattern of reactivity, each clone expressing a peculiar specificity. Our results suggest the possibility of isolating, from melanoma patients' PBL, T-cell clones with proliferative activity to Auto-Me and Auto-Me plus IL-2, and T-cell clones which apparently express both proliferative and cytotoxic activity to Auto-Me.
Fossati
G
G
Division of Experimental Oncology D, Istituto Nazionale Tumori, Milan, Italy.
Anichini
A
A
Squarcina
P
P
Mazzocchi
A
A
Parmiani
G
G
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002455:Cell Division; D002999:Clone Cells / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008545:Melanoma / Q000097:blood* / Q000276:immunology; D051379:Mice; D010641:Phenotype; D011961:Receptors, Fc / Q000032:analysis; D017452:Receptors, IgG
D000945:Antigens, Differentiation, T-Lymphocyte; D011961:Receptors, Fc; D017452:Receptors, IgG