Molecular cloning and characterization of the recA gene of Pseudomonas aeruginosa PAO.
The recA gene of Pseudomonas aeruginosa PAO has been isolated and introduced into Escherichia coli K-12. Resistance to killing by UV irradiation was restored in several RecA-E. coli K-12 hosts by the P. aeruginosa gene, as was resistance to methyl methanesulfonate. Recombination proficiency was also restored, as measured by HfrH-mediated conjugation and by the ability to propagate Fec-phage lambda derivatives. The cloned P. aeruginosa recA gene restored both spontaneous and mitomycin C-stimulated induction of lambda prophage in lysogens of a recA strain of E. coli K-12.
Kokjohn
T A
TA
Miller
R V
RV
D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000528:radiation effects; D011938:Rec A Recombinases / Q000235:genetics*; D013045:Species Specificity; D014466:Ultraviolet Rays
D011938:Rec A Recombinases; D004262:DNA Restriction Enzymes