Simultaneous electrophoretic determination of phosphoglucomutase subtypes, adenosine deaminase, erythrocyte acid phosphatase, and adenylate kinase enzyme phenotypes.
Many of the conventional agarose phosphoglucomutase (PGM) subtyping systems presently in use fail to provide a good separation between the 1 + and 2- bands as well as the 2+ band and the more anodic moving bands. Use of a 1-mm-thick gel composed of 1% ISO GEL (FMC Corp.) and phosphate-citric acid gel and tank buffers with a pH of 5.3 provided exceptionally good separation between all four of the major subtyping bands. The additional criteria for this procedure is a voltage of 21 V/cm and a run time of 4 h. Utilization of this procedure using case samples of varied ages proved the reliability of the procedure. Also examined were the effects of several reducing agents on the enzyme band patterns and the use of this system for the simultaneous determinations of the adenosine deaminase (ADA), erythrocyte acid phosphatase (EAP), and adenylate kinase (AK) enzyme phenotypes.
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D000135:Acid Phosphatase / Q000235:genetics*; D000243:Adenosine Deaminase / Q000235:genetics*; D000263:Adenylate Kinase / Q000235:genetics*; D001797:Blood Protein Electrophoresis; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics; D009700:Nucleoside Deaminases / Q000235:genetics*; D010641:Phenotype; D010733:Phosphoglucomutase / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*
D007527:Isoenzymes; D010770:Phosphotransferases; D000263:Adenylate Kinase; D000135:Acid Phosphatase; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D010733:Phosphoglucomutase