Identification of new atrial natriuretic peptides in frog heart.
It has been observed that mammalian atrial natriuretic peptide (ANP)-like immunoreactivity is found in frog heart, but to date the natriuretic factors have not yet been identified. Isolation from bull-frog heart extract was performed mainly by immunoaffinity chromatography on a column linked with anti-hANP IgG. From the low molecular weight fraction, 24- and 21-amino acid peptides were purified to homogeneity. Both peptides were found to elicit diuretic-natriuretic as well as vasorelaxant activity, and were named "frog ANP-24" and "frog ANP-21", respectively. Complete amino acid sequences of the peptides were established by microsequencing and confirmed by syntheses. Frog ANP-21 was identified as an N-terminally three amino acid deleted form of frog ANP-24. Remarkable sequence homology was observed between frog ANP and mammalian ANP, especially in the regions flanked by two half-cystine residues.
Sakata
J
J
Department of Biochemistry, Miyazaki Medical College, Japan.
Kangawa
K
K
Matsuo
H
H
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008970:Molecular Weight; D009206:Myocardium / Q000032:analysis*; D011892:Rana catesbeiana
D009320:Atrial Natriuretic Factor