Interleukin 1 stimulates its own receptor expression on human fibroblasts through the endogenous production of prostaglandin(s).
The regulation of interleukin 1 receptor (IL 1R) expression on human dermal fibroblasts was investigated. On exposure to IL 1 for 3 h at 37 degrees C, the capacity of fibroblasts to bind 125I-labeled human recombinant IL 1 alpha (125I-IL 1 alpha) was reduced by 75%. The IL 1 binding capability of the fibroblasts was restored to control levels by 16 h after removal of unbound IL 1, and then increased to about twofold over that of control cells by 48 h. This later enhancement of IL 1 receptor expression after IL 1 treatment was abolished by indomethacin. Addition of exogenous (PGE1 and PGE2, also analogues of AMP, or forskolin increased the specific binding of 125I-IL 1 alpha to fibroblasts. Scatchard analysis indicated that PGE2 increased the number of IL 1R from approximately 1.6 X 10(3) to 5.4 X 10(3) per cell without change in the binding affinity. These data suggest that the later IL 1-induced up-regulation of IL 1R is mediated by IL 1 stimulation of endogenous prostaglandin production. The combination of PGE2 and prednisolone increased the number of IL 1R on fibroblasts in an additive manner.
Akahoshi
T
T
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, Maryland 21701-1013.
Oppenheim
J J
JJ
Matsushima
K
K
D000249:Adenosine Monophosphate; D005576:Colforsin; D015232:Dinoprostone / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005938:Glucocorticoids; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D011453:Prostaglandins / Q000096:biosynthesis*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000187:drug effects*; D017472:Receptors, Interleukin-1
D005938:Glucocorticoids; D007375:Interleukin-1; D011453:Prostaglandins; D011500:Protein Synthesis Inhibitors; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D005576:Colforsin; D000249:Adenosine Monophosphate; D015232:Dinoprostone