Stress-induced secretion of adrenocorticotropin in rats is inhibited by administration of antisera to ovine corticotropin-releasing factor and vasopressin.
Intact handled rats were pretreated with the immunoglobulin G fractions from normal rabbit serum or antisera to ovine corticotropin-releasing factor (CRF) and/or vasopressin and subjected to restraint or formalin stress. The formalin-induced rise in plasma ACTH was reduced to 28% in rats pretreated with anti-CRF, to 53% in those pretreated with antivasopressin, and to 16% in rats given both antibodies. Pretreatment of animals with anti-CRF, antivasopressin, or a combination of both antibodies also attenuated the ACTH response to restraint stress to 13%, 37%, and 12%, respectively, of those in normal rabbit serum-treated rats. Antiserum pretreatment did not reduce the restraint- or formalin-induced rise in plasma PRL in the same animals, however. We conclude, therefore, that both vasopressin and an ovine CRF-like peptide are physiologically relevant peptides involved in stress-induced ACTH release.
Linton
E A
EA
Tilders
F J
FJ
Hodgkinson
S
S
Berkenbosch
F
F
Vermes
I
I
Lowry
P J
PJ
D000324:Adrenocorticotropic Hormone / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D001834:Body Water / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000276:immunology*; D005557:Formaldehyde / Q000494:pharmacology; D007106:Immune Sera / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012149:Restraint, Physical; D012756:Sheep; D013312:Stress, Physiological / Q000139:chemically induced / Q000378:metabolism*; D014667:Vasopressins / Q000276:immunology*
D007106:Immune Sera; D014667:Vasopressins; D005557:Formaldehyde; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone