Generation and characterization of a temperature-sensitive mutation in the NS-1 gene of the autonomous parvovirus minute virus of mice.
In-phase single-codon insertion mutations were constructed in the open reading frames of the NS-1 and NS-2 genes of the autonomous parvovirus minute virus of mice. A viral mutant containing an isoleucine insertion exclusively within NS-1 between residues 229 and 230 was isolated that produced approximately 3 orders of magnitude fewer plaques at 39 degrees C than at 32 degrees C. Preliminary characterization of the mutant demonstrated that the NS-1 gene product is independently required for both genome amplification and the regulation of the temporal expression between the two viral transcription units during lytic infection.
Tullis
G E
GE
Department of Microbiology, School of Medicine, University of Missouri-Columbia 65212.
Labieniec-Pintel
L
L
Clemens
K E
KE
Pintel
D
D
D002213:Capsid / Q000235:genetics*; D003062:Codon; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000235:genetics; D005784:Gene Amplification; D005786:Gene Expression Regulation; D005814:Genes, Viral; D008915:Minute Virus of Mice / Q000235:genetics* / Q000254:growth & development; D010321:Parvoviridae / Q000235:genetics*; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics; D013696:Temperature; D014758:Viral Core Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins; D014779:Virus Replication
D003062:Codon; D004279:DNA, Viral; D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins