title: Induction of nitroblue tetrazolium reduction in mouse peritoneal macrophages by tumour promoters and inhibition of the induced nitroblue tetrazolium reduction by some inhibitors.
abstract: Two polyacetates, aplysiatoxin and debromoaplysiatoxin, as well as 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein and teleocidin enhance nitroblue tetrazolium (NBT) reduction in mouse peritoneal macrophages in vitro. The ED50 values for NBT reduction of these 5 TPA-type tumor promoters were 4.2 ng/ml for TPA, 36 ng/ml for mezerein, 0.53 ng/ml for teleocidin, 1.5 ng/ml for aplysiatoxin and 108 ng/ml for debromoaplysiatoxin. The NBT reduction induced by the 5 tumor promoters is inhibited by 2 inhibitors of tumor promotion, retinoic acid and dibromoacetophenone. The possibility that tumor promotion by TPA-type tumor promoters involves similar mechanisms such as superoxide anion radicals release in cell membranes is discussed.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkawa
authors.forename: K
authors.initials: K
authors.lastname: Iwata
authors.forename: H
authors.initials: H
authors.lastname: Shibuya
authors.forename: N
authors.initials: N
authors.lastname: Inui
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D004224:Diterpenes; D005260:Female; D005609:Free Radicals; D008235:Lyngbya Toxins / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D000098:Acetophenones; D002273:Carcinogens; D004224:Diterpenes; D005609:Free Radicals; D008235:Lyngbya Toxins; D013729:Terpenes; D013778:Tetrazolium Salts; D013481:Superoxides; C022176:teleocidins; D009580:Nitroblue Tetrazolium; C011309:mezerein; C017179:debromoaplysiatoxin; C006610:aplysiatoxin; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate; C006821:4-bromophenacyl bromide