Isolation of ovine rotavirus in cell cultures. Brief report.
Three cytopathic rotavirus strains were isolated in MA 104 cells from intestinal contents of lambs with diarrhea. Pretreatment of virus with pancreatin and incorporation of a small amount of pancreatin in maintenance medium were important for establishment of the strains in these cells. The isolates showed marked cross reactions with bovine rotavirus in immunofluorescence, but no cross reaction with bovine, human, simian, equine, porcine and lapine rotaviruses in neutralization.
Makabe
T
T
Komaniwa
H
H
Kishi
Y
Y
Yataya
K
K
Imagawa
H
H
Sato
K
K
Inaba
Y
Y
D000818:Animals; D002460:Cell Line; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004622:Embryo, Mammalian; D005766:Gastrointestinal Contents / Q000032:analysis; D007668:Kidney; D008253:Macaca mulatta; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000382:microbiology / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D014776:Virus Cultivation