Adenovirus transcriptional complexes contain EIa encoded tumour antigens physically bound to cellular proteins.
Adenovirus type 12 transcriptional complexes were isolated from cells during the early phase of infection. Sedimentation analysis identified a fast sedimenting complex type I and a slow sedimenting complex type II. Both complexes made virus specific RNA complementary to all the early genes and both contained viral DNA, which in type II but not in type I had nucleosome like configuration. Analysis of the proteins of the complexes with antiserum against Ad 12 EIa-beta-galactosidase fusion protein expressed in E. coli demonstrated the following: (a) type I complex contained EIa 45 K protein, which co-precipitated with cellular proteins of mol. wt. 42, 58, and 60 K, (b) type II complex contained EIa 47 K protein, which co-precipitated with major cellular proteins of 35, 40-46 K and minor proteins of 58, 60, 68, 76, 86, and 120-150 K. Association of EIa specific and cellular proteins to transcriptional complexes was sensitive to both 1 M NaCl and DNAse I indicating the DNA binding nature of these proteins. Treatment of transcriptional complexes with 1 M NaCl or DNase I released EIa proteins, which still remained strongly bound to cellular proteins. These findings suggested that EIa proteins bind to viral DNA and that this binding is probably mediated by cellular proteins.
Mirza
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Institut für Molekularbiologie (Tumorforschung), Universitätsklinikum Essen, Federal Republic of Germany.
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000032:analysis*; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009687:Nuclear Proteins / Q000032:analysis; D009707:Nucleosomes; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D011233:Precipitin Tests; D012367:RNA, Viral / Q000032:analysis
D017456:Adenovirus Early Proteins; D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009707:Nucleosomes; D009856:Oncogene Proteins, Viral; D012367:RNA, Viral