Removal of a 67-base-pair sequence in the noncoding region of protooncogene fos converts it to a transforming gene.
Transformation of fibroblasts by protooncogene fos (c-fos) requires the linkage of viral long terminal repeat (LTR) sequences and interruption of 3'-noncoding sequences. We have identified an A + T-rich stretch of 67 nucleotides, located 627-693 base pairs downstream from the coding domain and 123-189 base pairs upstream from the putative poly(A) addition site, removal of which confers transforming activity to the c-fos gene. A novel regulation of the expression of the c-fos gene is proposed, which may be functional in vivo to prevent the gene from becoming an oncogene.
Meijlink
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Curran
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Miller
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Verma
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D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D002872:Chromosome Deletion; D005786:Gene Expression Regulation; D051379:Mice; D009154:Mutation; D009690:Nucleic Acid Conformation; D009857:Oncogenes; D011061:Poly A / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D009053:Sarcoma Viruses, Murine / Q000235:genetics*
D011061:Poly A