Sequential ADP-ribosylation pattern of nucleosomal histones. ADP-ribosylation of nucleosomal histones.
The pattern of nucleosomal histones poly(ADP-ribosyl)ation is changed under conditions which affect the poly(ADP-ribosyl)ation state of the enzyme. At low NAD concentrations the enzyme can poly(ADP-ribosyl)ate histones H1 and H1, H2A, A2A, and H2B. However at NAD concentrations above 10 microM the enzyme preferentially poly(ADP-ribosyl)ates histone H1 to a hyper ADP-ribosylated form. Furthermore we have observed hyper ADP-ribosylation of histone H2B at NAD concentrations of 10 microM suggesting that histone H2B can undergo the same type of ADP-ribosylation pattern as histone H1. Also at higher NAD concentrations an elongation of the polymer attached to the enzyme and other nuclear proteins takes place.
Huletsky
A
A
Niedergang
C
C
Fréchette
A
A
Aubin
R
R
Gaudreau
A
A
Poirier
G G
GG
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002843:Chromatin / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D006657:Histones / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology*; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D009707:Nucleosomes / Q000201:enzymology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D013950:Thymus Gland / Q000201:enzymology
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D009243:NAD; D011065:Poly(ADP-ribose) Polymerases; D009244:NAD+ Nucleosidase