Cloning of the contiguous 165-kilobase-pair region around the terminus of Escherichia coli K-12 DNA replication.
Escherichia coli K-12 chromosomal DNA was partially digested with either EcoRI or HindIII, and cosmid libraries were constructed. By screening these libraries, a series of partially overlapping clones which covered the terC region was isolated. The cloned area spanned about 165 kilobase pairs, corresponding to the 29.7-to-33.2-min region of the genetic map of the E. coli chromosome.
Asada
K
K
Nakatani
S
S
Takanami
M
M
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D003001:Cloning, Molecular; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes