Amelioration of hydroxyurea-induced suppression of phagocytosis in human granulocytes by free radical scavengers.
The exposure of human circulatory white cells in vitro to 0.1-1-10 mol/l hydroxyurea (HU) for 20 h induced a progressive dose-dependent suppression of the phagocytic activity of granulocytes. The suppressing effect of 20 h exposure to 1 mol/l HU was used to examine the protection afforded by free radical scavengers against HU-induced cytotoxicity. It has been found that, in the suitable concentration of the protecting agent, a substantial protective effect of sodium benzoate, acetylosalicylic acid, alpha-tocopherol, ascorbic acid, catalase, peroxidase or superoxide dismutase can be achieved.
Szczepańnska
I
I
Kwiatkowska
J
J
Przybyszewski
W M
WM
Sitarska
E
E
Malec
J
J
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D019817:Benzoic Acid; D002374:Catalase / Q000494:pharmacology; D003864:Depression, Chemical; D005609:Free Radicals; D006098:Granulocytes / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D007527:Isoenzymes / Q000494:pharmacology; D009195:Peroxidase; D010544:Peroxidases / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D001565:Benzoates; D005609:Free Radicals; D007527:Isoenzymes; D014810:Vitamin E; D019817:Benzoic Acid; D010544:Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; D001241:Aspirin; D006918:Hydroxyurea