In vivo transfer of pAM beta 1 from Lactobacillus reuteri to Enterococcus faecalis.
Trials were conducted to determine the in vivo transferability of plasmid-mediated antibiotic resistance between two strains of enteric Gram-positive bacteria. Germ-free mice were associated with the donor Lactobacillus reuteri DSM 20016 strain, carrying the broad host range pAM beta 1 plasmid, and with the Enterococcus faecalis JH2SS recipient strain. Analysis of faecal content of associated mice demonstrated that the in vivo transfer of this plasmid did occur and that frequencies of conjugation were affected by the presence of subtherapeutic levels of antibiotic in the diet.
Morelli
L
L
Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Piacenza, Italy.
Sarra
P G
PG
Bottazzi
V
V
D000818:Animals; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology; D005243:Feces / Q000382:microbiology; D005856:Germ-Free Life; D007778:Lactobacillus / Q000187:drug effects / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D011815:R Factors; D000198:Spectinomycin / Q000494:pharmacology
D004269:DNA, Bacterial; D004917:Erythromycin; D000198:Spectinomycin