Cloned and expressed human Fc receptor for IgG mediates anti-CD3-dependent lymphoproliferation.
We have utilized gene transfer experiments to investigate the role of a human monocyte receptor for IgG (Fc gamma RII) in mouse IgG1 anti-CD3 (Leu 4)-induced lymphoproliferation in vitro. Mouse Ltk- cells expressing human Fc gamma RII or a mutant of Fc gamma RII lacking the entire cytoplasmic domain of the receptor mediate anti-CD3-induced lymphoproliferation in cultures of adherent cell-depleted human PBMC. Expression of an Fc gamma RII mutant lacking transmembrane and cytoplasmic domains (soluble Fc gamma RII) in COS7 cells yielded a secreted receptor which retained affinity for IgG, even in the absence of the mutant receptor's N-linked oligosaccharides. Soluble Fc gamma RII inhibits rosette formation by human IgG-sensitized RBC and the Fc gamma RII-bearing cell line K562, but does not sitmulate anti-CD3-induced lymphoproliferation under the conditions tested.
Peltz
G A
GA
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304.
Trounstine
M L
ML
Moore
K W
KW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000943:Antigens, Differentiation / Q000235:genetics / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D002460:Cell Line; D002474:Cell-Free System; D000882:Haplorhini; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007166:Immunosuppressive Agents / Q000502:physiology; D008077:Lipoproteins, LDL / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D009154:Mutation; D011961:Receptors, Fc / Q000235:genetics / Q000502:physiology*; D017452:Receptors, IgG; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D008077:Lipoproteins, LDL; D011961:Receptors, Fc; D017452:Receptors, IgG; C022060:rosette-inhibiting factor