[Effect of freezing and thawing on the structure and function of cytochrome oxidase].
The effect of freezing-thawing on cytochrome oxidase from cattle heart was studied. The enzyme was characterized for its activity, absorption spectra, temperature perturbation differential spectra and conformational transitions. A decrease in the activity and conformational changes depend on the composition of the buffer system and the presence of potassium and sodium chlorides in the solution.
Rozanova
E D
ED
Moiseev
V A
VA
Naumenko
E I
EI
D000818:Animals; D002021:Buffers; D002417:Cattle; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005615:Freezing; D066298:In Vitro Techniques; D009206:Myocardium / Q000201:enzymology*; D011487:Protein Conformation; D013053:Spectrophotometry; D014021:Tissue Preservation
D002021:Buffers; D003576:Electron Transport Complex IV
Vliianie zamorazhivaniia-otogreva na strukturu i funktsiiu tsitokhromoksidazy.