title: [Isolation and purification of restriction endonuclease PmiI from Proteus mirabilis 1667].
abstract: A new restriction endonuclease Pmi I was detected in Proteus mirabilis 1667. The enzyme hydrolyzes DNA of the phage lambda into 10 electrophoretically separating fragments with molecular weights of 1.3-7.9 mD. With the use of two-stage chromatography on blue sepharose and phosphocellulose it is possible to obtain restriction endonuclease Pmi I free of the admixtures of ballast proteins, nonspecific nucleases and phosphatases.
authors.forename: N L
authors.initials: NL
authors.lastname: Bakh
authors.forename: N V
authors.initials: NV
authors.lastname: Tsvetkova
authors.forename: I E
authors.initials: IE
authors.lastname: Semina
authors.forename: A P
authors.initials: AP
authors.lastname: Tarasov
authors.forename: M M
authors.initials: MM
authors.lastname: Mileĭkovskaia
mesh_terms: D010582:Bacteriophage lambda; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; D011513:Proteus mirabilis / Q000201:enzymology*
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C046158:endodeoxyribonuclease PmiI; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Vydelenie i ochistka restriktsionnoĭ éndonukleasy PmiI iz shtamma Proteus mirabilis 1667.