Transposon-like sequences in extrachromosomal circular DNA from mouse thymocytes.
Small polydisperse circular (spc) DNA was isolated from mouse thymocytes and cloned into the HindIII site of lambda vector Charon 7. Fifty-six recombinants from this spc DNA library were analyzed. R repeats, which were originally found near immunoglobulin genes, were enriched in spc DNA clones relative to their representation in the chromosome. In one clone, the R sequence was linked to Bam and MIF sequences and the contiguous arrangement was truncated from both ends. In another clone, composite Bam/R and R repeats existed as a pair in inverted repeat orientation. Truncation occurred from the 5' side without affecting the 3' ends. In both clones, short direct repeats flanked the repeated sequences. The possible role of R sequences in transposition and circular formation is discussed.
Fujimoto
S
S
Tsuda
T
T
Toda
M
M
Yamagishi
H
H
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004270:DNA, Circular / Q000032:analysis*; D007136:Immunoglobulins / Q000235:genetics; D051379:Mice; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000032:analysis
D004251:DNA Transposable Elements; D004270:DNA, Circular; D007136:Immunoglobulins