title: Demonstration of receptors for a PDGF-like mitogen on human osteosarcoma cells.
abstract: U-2 OS human osteosarcoma cells synthesize, process and secrete a platelet-derived growth factor (PDGF)-like mitogen. Incubation of these cells with 1 mM suramin unmasks PDGF receptor sites which are normally occupied or down regulated by the secreted endogenous PDGF-like mitogen. Partially purified preparations of metabolically labelled U-2 OS conditioned medium binds to U-2 OS cells and binding is inhibited by excess PDGF. These findings suggest that U-2 OS cells are capable of autocrine stimulation.
authors.forename: D T
authors.initials: DT
authors.lastname: Graves
authors.forename: A J
authors.initials: AJ
authors.lastname: Owen
authors.forename: H N
authors.initials: HN
authors.lastname: Antoniades
mesh_terms: D001667:Binding, Competitive; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D012516:Osteosarcoma / Q000032:analysis*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis*; D017479:Receptors, Platelet-Derived Growth Factor; D013498:Suramin / Q000494:pharmacology
chemical_list: D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D013498:Suramin; D004815:Epidermal Growth Factor; D017479:Receptors, Platelet-Derived Growth Factor