title: The DNA between Rz and cosR in bacteriophage lambda is nonessential.
abstract: Near the right end of phage lambda DNA, between gene Rz and the cos site, are 2050 bp of apparently non-coding DNA. We have cloned a lambda DNA fragment containing this DNA into a plasmid and constructed a deletion, omega l, extending from a site within the Rz gene to a site about 560 bp from cos. This deletion could be recombined into viable lambda phage at a frequency equal to that observed for the undeleted sequence. Recombinant phage lambda carrying the omega l deletion were demonstrated to have the same burst size and kinetics of phage production as undeleted lambda. The omega l deletion can be used to extend the capacity of lambda cloning vectors and to provide a region for the insertion of heterologous DNA which should exhibit controllable high level expression from the lambda late promoter, p'R.
authors.forename: V J
authors.initials: VJ
authors.lastname: Hernandez
authors.forename: T D
authors.initials: TD
authors.lastname: Edlind
authors.forename: R F
authors.initials: RF
authors.lastname: Young
authors.forename: G M
authors.initials: GM
authors.lastname: Ihler
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D008242:Lysogeny; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes