title: Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts.
abstract: Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.
authors.affiliation: Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
authors.forename: P E
authors.initials: PE
authors.lastname: Nielsen
authors.forename: C
authors.initials: C
authors.lastname: Jeppesen
authors.forename: O
authors.initials: O
authors.lastname: Buchardt
mesh_terms: D010582:Bacteriophage lambda / Q000032:analysis; D001483:Base Sequence; D004247:DNA / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004278:DNA, Superhelical / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D009875:Operator Regions, Genetic; D009942:Organometallic Compounds / Q000494:pharmacology*; D010777:Photochemistry; D010957:Plasmids; D011506:Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D014466:Ultraviolet Rays; D014501:Uranium / Q000494:pharmacology*; D014502:Uranyl Nitrate / Q000494:pharmacology*
chemical_list: D004278:DNA, Superhelical; D009942:Organometallic Compounds; D011506:Proteins; D012097:Repressor Proteins; D014502:Uranyl Nitrate; C005460:uranyl acetate; D014501:Uranium; D004247:DNA