title: Oncogene expression in T-cell lymphoproliferative disorders.
abstract: We have investigated the expression of oncogenes and other related genes in eleven patients with T-cell lymphoproliferative disorders and ten patients with other hematologic malignancies. The phenotypes of the T-cell disorders were determined using monoclonal antibodies specific for helper or suppressor subsets. RNA preparations were isolated from peripheral blood mononuclear cells and/or lymph node sections, 5'-end labeled with gamma-32P-ATP, and hybridized under stringent conditions to an excess of nitrocellulose-bound specific cloned DNA; autoradiographs were analysed by microdensitometry. Results revealed increased expression of K-ras, v-fps, transferrin receptor, alpha-tubulin and alpha-interferon in at least five of six helper T-cell lymphoproliferative disorders, while five of five suppressor T-cell disorders demonstrated levels of hybridization to these clones no higher than background. However, studies of T-suppressor disorders demonstrated enhanced levels of beta-interferon-specific RNA in five of five patients, an increase apparent in three of six T-helper chronic lymphoproliferative disorders. These results demonstrate different patterns of gene expression evident in T-helper and T-suppressor abnormalities.
authors.affiliation: Dept. of Immunology, Mayo Clinic, Rochester, MN 55905.
authors.forename: G E
authors.initials: GE
authors.lastname: Woloschak
authors.forename: W C
authors.initials: WC
authors.lastname: Hooper
authors.forename: M J
authors.initials: MJ
authors.lastname: Doerge
authors.forename: R L
authors.initials: RL
authors.lastname: Phyliky
authors.forename: T E
authors.initials: TE
authors.lastname: Witzig
authors.forename: P M
authors.initials: PM
authors.lastname: Banks
authors.forename: G W
authors.initials: GW
authors.lastname: Dewald
authors.forename: C Y
authors.initials: CY
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D005260:Female; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D008223:Lymphoma / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D013601:T-Lymphocytes; D006377:T-Lymphocytes, Helper-Inducer; D050378:T-Lymphocytes, Regulatory
chemical_list: D000911:Antibodies, Monoclonal