Purification of a new restriction endonuclease, StyI, from Escherichia coli carrying the hsd+ miniplasmid.
A new restriction endonuclease, StyI, free of contaminating nuclease activities, has been isolated from Escherichia coli carrying the hsd+ miniplasmid of Salmonella typhi origin. In the presence of 10 mM Mg2+, it recognizes and cleaves a hexanucleotide sequence of 5'-C decreases C(AT)(AT)GG. The advantages of the StyI endonuclease include its stability, high yield (more than 2 X 10(3) units/g of wet cells), easy handling of producer cells, and the ability to recognize new sequences, CCAAGG and CCTTGG.
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D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D012485:Salmonella typhi / Q000201:enzymology / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C045874:endodeoxyribonuclease StyI; D015252:Deoxyribonucleases, Type II Site-Specific