Selective recycling of the mannose 6-phosphate/IGF-II receptor to the trans Golgi network in vitro.
Mannose 6-phosphate receptors carry soluble lysosomal enzymes from the trans Golgi network (TGN) to prelysosomes, and then return to the TGN for another round of lysosomal enzyme sorting. We describe here a complementation scheme that detects the vesicular transport of the 300 kd mannose 6-phosphate/IGF-II receptor from prelysosomes to the TGN in cell extracts. In vitro transport displays the same selectivity observed in living cells in that the transferrin receptor traverses to the TGN at a much lower rate than mannose 6-phosphate receptors. Furthermore, recycling of mannose 6-phosphate/IGF-II receptors to the TGN requires GTP hydrolysis and can be distinguished biochemically from the constitutive transport of proteins between Golgi cisternae by its resistance to the weak base, primaquine.
Goda
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Y
Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.
Pfeffer
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SR
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D006224:Cricetinae; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D006868:Hydrolysis; D037181:Lectins, C-Type; D008247:Lysosomes / Q000201:enzymology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011319:Primaquine / Q000494:pharmacology; D011972:Receptor, Insulin / Q000378:metabolism*; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000378:metabolism*; D017451:Receptors, Somatomedin; D011990:Receptors, Transferrin / Q000378:metabolism
D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D017451:Receptors, Somatomedin; D011990:Receptors, Transferrin; D006160:Guanosine Triphosphate; D011972:Receptor, Insulin; D011319:Primaquine