Lipopolysaccharide-induced suppression of graft-versus-host reactivity in mice.
Reconstitution of lethally irradiated mice with spleen cells from donors that had been treated with lipopolysaccharide (LPS) intravenously and allogeneic spleen cells subcutaneously leads to a suppressed anti-host delayed-type hypersensitivity (DTH). Either donor injection alone proved to be ineffective. The state of suppression appeared to be antigen-specific, but, depending on the experimental conditions, also anti-host DTH to third-party alloantigens could be suppressed. The suppression was mediated by a population of Thy-1- suppressor cells that could also be induced in athymic nude mice. The suppressor cells specifically adhered to anti-kappa-coated plastic plates, but were not adsorbed by passage through a Sephadex G-10 column. Thus, it appears that the combined donor treatment with LPS and allogeneic spleen cells induces a population of B cells that can suppress anti-host immune reactivity.
Molendijk
A
A
Department of Cell Biology, Erasmus University, Rotterdam, The Netherlands.
Knulst
A C
AC
van Gurp
R J
RJ
van Oudenaren
A
A
Benner
R
R
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006087:Graft vs Host Reaction / Q000187:drug effects*; D006968:Hypersensitivity, Delayed; D007114:Immunization; D007165:Immunosuppression Therapy; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D008070:Lipopolysaccharides