'Red pigment' from ADE-2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases.
Protection of DNA from cleavage by restriction endonucleases EcoRI, HindIII, BamHI, and Bg/II with red pigment, produced by ADE-2 mutants of Saccharomyces cerevisiae is demonstrated. Purification of yeast DNA from pigment can be achieved by chromatography on hydroxyapatite columns.
Meskauskas
A
A
Ksenzenko
V
V
Shlyapnikov
M
M
Kryukov
V
V
Citavicius
D
D
D000225:Adenine / Q000096:biosynthesis*; D001426:Bacterial Proteins; D010582:Bacteriophage lambda; D004396:Coloring Agents / Q000494:pharmacology*; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors*; D004271:DNA, Fungal / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D004396:Coloring Agents; D004271:DNA, Fungal; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific; D000225:Adenine