title: The domain structure of tryptophan synthase. A neutron scattering study.
abstract: Tryptophan synthase from Escherichia coli is a complex of two alpha subunits and two beta subunits. Small-angle neutron scattering involving deuterium-labelled isomers revealed the quaternary structure of the enzyme at the level of the beta 2 subunit and the two structural domains P1 and P2 which constitute the alpha subunits. Within the alpha 2 beta 2 complex, the two alpha subunits are completely separated. They are situated on opposite sides of the beta 2 subunit. The most probable distance between the two alpha protomers is 10.5 +/- 1 nm; the nearest distance is 5.8 +/- 0.5 nm, and the largest distance is 13.5 +/- 0.5 nm. The two domains of the same alpha subunit are intimately juxtaposed. The distances between two like or unlike domains belonging to opposite alpha subunits are roughly equal. All domains exhibit about equal distances to the beta 2 subunit which is situated in the centre of the complex. Thus the cleft between P1 and P2, which probably contains the active site of the alpha subunit, makes intimate contact with the beta 2 subunit. Neutron scattering allows us to determine the shape of the beta 2 subunit within the complex. Comparison with the free dimer suggests a conformational change, upon assembly, from an elongated into a more compact form.
authors.forename: K
authors.initials: K
authors.lastname: Ibel
authors.forename: R P
authors.initials: RP
authors.lastname: May
authors.forename: K
authors.initials: K
authors.lastname: Kirschner
authors.forename: A N
authors.initials: AN
authors.lastname: Lane
authors.forename: H
authors.initials: H
authors.lastname: Szadkowski
authors.forename: M T
authors.initials: MT
authors.lastname: Dauvergne
authors.forename: M
authors.initials: M
authors.lastname: Zulauf
mesh_terms: D003903:Deuterium; D004926:Escherichia coli / Q000201:enzymology; D008027:Light; D046911:Macromolecular Substances; D009502:Neutrons; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D055503:Protein Multimerization; D011522:Protons; D012542:Scattering, Radiation; D014367:Tryptophan Synthase
chemical_list: D046911:Macromolecular Substances; D010446:Peptide Fragments; D011522:Protons; D003903:Deuterium; D014367:Tryptophan Synthase