Physicochemical studies of the iron(III)-carminomycin complex and evidence of the lack of stimulated superoxide production by NADH dehydrogenase.
Fe(III) complex of an antitumoral antibiotic carminomycin has been studied. Using potentiometric and spectroscopic measurements we have shown that carminomycin forms with Fe(III) a well-defined species in which three molecules of drug are chelated to one Fe(III) ion. This occurs with the release of one proton per molecule of drug. Magnetic susceptibility measurements suggest that six oxygen atoms are bound to iron. The stability constant is 3 X 10(34). The in vitro inhibition of P 388 leukemia cell growth by this complex compares with that of the free drug. This complex, unlike the free drug, does not catalyze the flow of electrons from NADH to molecular oxygen through NADH dehydrogenase.
Fiallo
M M
MM
Garnier-Suillerot
A
A
D000818:Animals; D002360:Carubicin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002942:Circular Dichroism; D003579:Cytochrome Reductases / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism; D003630:Daunorubicin / Q000031:analogs & derivatives; D004355:Drug Stability; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology; D007941:Leukemia P388 / Q000473:pathology; D051379:Mice; D009206:Myocardium; D009245:NADH Dehydrogenase / Q000378:metabolism*; D009942:Organometallic Compounds; D011199:Potentiometry; D013053:Spectrophotometry; D013481:Superoxides / Q000378:metabolism*
D003574:Cytochrome c Group; D009942:Organometallic Compounds; C045654:carminomycin-iron complex; D013481:Superoxides; D007501:Iron; D002360:Carubicin; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase; D003630:Daunorubicin