Analysis of parapoxvirus genomes.
Eight stomatitis papulosa (SP), four orf and two milker's nodes (MN) virus isolates were compared by restriction enzyme analysis. Considerable genetic heterogeneity was found not only between isolates belonging to the three different taxonomic groups but also between members of the same group. This heterogeneity precludes classification of parapoxviruses simply by comparison of their DNA cleavage patterns. Restriction maps were therefore prepared for 12 parapoxvirus DNAs. Fragments from defined regions of the genome were then selected and used as probes for cross-hybridizations to all other parapoxvirus DNAs. DNA fragments derived from an internal region of the genome hybridized strongly to all parapoxvirus isolates examined. In contrast, cross-hybridization of the end region of the DNA molecule was observed only between members of the same virus group. Molecular hybridization as a means of classifying parapoxvirus isolates is discussed.
Gassmann
U
U
Wyler
R
R
Wittek
R
R
D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005814:Genes, Viral; D009693:Nucleic Acid Hybridization; D009923:Orf virus / Q000235:genetics; D010802:Phylogeny; D011212:Poxviridae / Q000145:classification / Q000235:genetics*; D010285:Pseudocowpox Virus / Q000235:genetics
D004279:DNA, Viral; D004262:DNA Restriction Enzymes