Isolation of the human glyceraldehyde-3-phosphate dehydrogenase gene by hybridization with synthetic oligonucleotides.
By screening a human genomic library from human lymphocyte DNA cloned in EMBL 3 vector with synthetic oligonucleotides homologous to the human GAPD cDNA 3'-non-coding region as probes, a unique lambda recombinant clone (EMBL-G5) was isolated at the Tm value. Preliminary restriction analysis and sequence data proved that this recombinant clone is the human structural gene for the glyceraldehyde- 3-phosphate dehydrogenase.
Martinelli
R
R
Istituto di Scienze Biochimiche, II Facoltà di Medicina e Chirurgia, Napoli.
Arcari
P
P
Salvatore
F
F
D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D004274:DNA, Recombinant; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics; D011544:Pseudogenes
D004274:DNA, Recombinant; D009841:Oligonucleotides; D004247:DNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases