Identification and immunolocalization of the laminin binding protein from embryonic avian corneal epithelial cells.
We report the identification of a 65-kDa laminin-binding protein (LBP) on the basal cell surface of embryonic corneal epithelium in chicken. The 65-kDa LBP was isolated by affinity chromatography with laminin-Sepharose. When reconstituted with lipid vesicles, it demonstrated specific binding for laminin. We produced monoclonal antibodies (MAbs) against 65-k Da LBP; these MAbs immunohistochemically localized to the basal epithelial cell surface. One MAb interfered with the binding of laminin to isolated epithelia and purified 65-k Da LBP. It appears that we have identified, at least in part, a cell-surface binding site for laminin. This site would provide the important link between the extracellular laminin and the intracellular cytoskeleton, and potentially the metabolic machinery of the corneal epithelial cell.
Sugrue
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Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D003315:Cornea / Q000166:cytology / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D007150:Immunohistochemistry; D008970:Molecular Weight; D011971:Receptors, Immunologic / Q000378:metabolism*; D017474:Receptors, Laminin
D000911:Antibodies, Monoclonal; D011971:Receptors, Immunologic; D017474:Receptors, Laminin