[Identification of the number of mutant copies of the genetic transfer factor AP42].
The copy number mutant of genetic transfer factor pAP42 (incFIX) was induced by the treatment of E. coli AP115 cells carrying the plasmid by N-methyl-N-nitro-N-nitrosoguanidine. The copy number mutant pAP42::Tn1cop1 is characterized by the increased copy number. The cells carrying the copy number mutant have a higher ampicillin resistance and higher beta-lactamase activity. Mutant plasmid pAP42: :Tn1cop1 is incompatible with plasmid pAP42 and compatible with plasmids of the other inc-groups of F-like plasmids.
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D002876:Chromosomes, Bacterial / Q000187:drug effects; D004251:DNA Transposable Elements / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D005815:Genetic Code / Q000187:drug effects; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation; D011815:R Factors / Q000187:drug effects; D001618:beta-Lactamases / Q000235:genetics
D004251:DNA Transposable Elements; D008769:Methylnitronitrosoguanidine; D001618:beta-Lactamases
Identifikatsiia mutantov kopiĭnosti faktora geneticheskogo perenosa pAP42.