Alterations in lactate dehydrogenase isoenzymes and phosphofructokinase activity in the hormonally induced G-XII cell line.
G-XII cell line was earlier developed in our laboratory from the granulosa lutein cells of goat ovaries, subjected to sustained stimulation by ovine luteinizing hormone. It is a non-clonogenic and non-tumorigenic cell line which secretes progesterone. In the present paper, we report some biochemical findings on the cell line studied between passages 10 and 15. The cells exhibited a shift in the lactate dehydrogenase isoenzyme pattern from the heart form to muscle form and a 300% increase in the phosphofructokinase activity compared to fresh granulosa cells.
Malik
A K
AK
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
Sharma
N
N
Chandiok
P
P
Chapekar
T N
TN
D000818:Animals; D002460:Cell Line; D005260:Female; D005812:Genes, Switch; D006107:Granulosa Cells / Q000187:drug effects / Q000201:enzymology*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D010732:Phosphofructokinase-1 / Q000378:metabolism*
D007527:Isoenzymes; D007986:Luteinizing Hormone; D007770:L-Lactate Dehydrogenase; D010732:Phosphofructokinase-1