Determination of cytotoxic T lymphocyte activity by time-resolved fluorometry using europium-labelled concanavalin A-stimulated cells as targets.
Time-resolved fluorometry was used to detect the cytolysis of concanavalin A-stimulated blast cells by employing cells stimulated in a mixed lymphocyte culture as effectors. The target cells were labelled with europium diethylenetriaminopentaacetate (EuDTPA) chelates. The results obtained showed that this method, which has been successfully applied to the measurement of natural killer cell activity, was also applicable to the more specific cytotoxic T lymphocyte reaction. The specific release was higher with EuDTPA-labelled target cells than with 51chromium-labelled target cells. As with 51chromium, EuDTPA can be used to distinguish between the cytolysis of specific and non-specific target cells.
Granberg
C
C
Department of Medical Microbiology, Turku University, Finland.
Blomberg
K
K
Hemmilä
I
I
Lövgren
T
T
D002860:Chromium Radioisotopes / Q000378:metabolism; D003208:Concanavalin A; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D005063:Europium / Q000378:metabolism; D005470:Fluorometry / Q000379:methods; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods; D004369:Pentetic Acid / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors
D002860:Chromium Radioisotopes; D003208:Concanavalin A; D005063:Europium; D004369:Pentetic Acid