Regulatory effects of IgG-BF on hybridoma B cells. molecular characterization of variant cell lines.
Immunoglobulin G-binding factors (IgG-BF) produced by mouse T cells or hybridoma T cells (T2D4) were used to manipulate in vitro mouse hybridoma B cells. Both IgG production by, and proliferation of, these cells was inhibited by IgG-BF, or during co-cultures with IgG-BF-producing T2D4 cells. Thus, treatment of tumor B cells, besides its potential therapeutic use, represents an invaluable model for studying the regulation of Ig production by IgG-BF at a molecular level. To further analyze the molecular events induced by IgG-BF in B cell hybridomas, a set of variant clones of a hybridoma cell line (UN2) was isolated and variants were characterized for their Ig production and their Fc gamma R expression.
Brunati
S
S
Laboratoire d'Immunologie Cellulaire et Clinique, INSERM U.255, Paris, France.
Miossec
C
C
Mathiot
C
C
Moncuit
J
J
Amigorena
S
S
Teillaud
J L
JL
Fridman
W H
WH
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015152:Blotting, Northern; D015153:Blotting, Western; D002455:Cell Division; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc / Q000032:analysis; D017452:Receptors, IgG
D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc; D017452:Receptors, IgG; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors