Sequential onset of permeability changes in mouse ascites cells induced by Sendai virus.
The addition of haemolytic Sendai virus to cells induces membrane changes in the following sequence: (i) Increased permeability to ions, (ii) increased permeability to low molecular weight metabolites, (iii) increased permeability to proteins. The consequences of an increased permeability to ions are: (a) alteration of membrane potential, (b) net changes in intracellular cations and (c) cell swelling, in that order. Depending on virus: cell ratio, Ca2+ concentration and temperature, it is possible to observe ion leakage without metabolite or protein leakage, and ion and metabolite leakage without protein leakage. A model for the induction of permeability changes is presented.
Bashford
C L
CL
Micklem
K J
KJ
Pasternak
C A
CA
D000818:Animals; D001201:Ascites / Q000473:pathology*; D002118:Calcium / Q000378:metabolism; D002463:Cell Membrane Permeability; D003588:Cytopathogenic Effect, Viral; D007093:Imidazoles / Q000494:pharmacology; D008564:Membrane Potentials; D051379:Mice; D008970:Molecular Weight; D010222:Parainfluenza Virus 1, Human; D010539:Permeability; D011506:Proteins / Q000378:metabolism; D012413:Rubidium / Q000378:metabolism
D007093:Imidazoles; D011506:Proteins; C031938:calmidazolium; D012413:Rubidium; D002118:Calcium