title: Post-translational modification of the beta-subunit of the human fibronectin receptor.
abstract: Monoclonal antibody DH12, directed against the beta-subunit of the fibronectin receptor recognizes a doublet of proteins (100 and 110 kDa) in Western blots of solubilized whole fibroblasts. Pulse-chase experiments with [35S]methionine in human skin fibroblasts suggested that the two proteins might be metabolically related as precursor (100 kDa) and product (110 kDa). Endo H digestion and [3H]fucose labeling suggested that maturation converted the high-mannose oligosaccharides (100 kDa) to the endoglycosidase H resistant complex type (110 kDa). This was supported by N-glycanase digestion and by chemical deglycosylation which showed a single polypeptide. Surface iodination of intact cells labeled only the presumed mature beta-subunit.
authors.affiliation: Center for Human Genetics, University of Leuven, Campus Gasthuisberg, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Jaspers
authors.forename: B
authors.initials: B
authors.lastname: de Strooper
authors.forename: M
authors.initials: M
authors.lastname: Spaepen
authors.forename: F
authors.initials: F
authors.lastname: van Leuven
authors.forename: G
authors.initials: G
authors.lastname: David
authors.forename: H
authors.initials: H
authors.lastname: van den Berghe
authors.forename: J J
authors.initials: JJ
authors.lastname: Cassiman
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011498:Protein Precursors; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic