title: Molecular probes define different regions of the mouse t complex.
abstract: Four genomic clones obtained from microdissected fragments of the proximal portion of mouse chromosome 17 have been used to identify a series of t-haplotype-specific restriction fragments. Their specificity is defined by presence in eight complete t haplotypes and absence from 18 inbred strains of wild-type mice. Partial t haplotypes contain subsets of the t-specific fragments, and each can be classified according to the t-specific fragments it contains. This is the first molecular evidence that independent partial t haplotypes contain different lengths of t haplotype DNA. Recombination studies indicate that partial t haplotypes suppress recombination in proportion to the extent of t haplotype DNA they contain. Molecular analysis of partial t haplotypes shows that the t-specific fragments map to and thus define different regions of the t complex. Certain regions of t haplotype DNA defined by t-specific restriction fragments can be correlated with loci involved in the control of transmission ratio distortion.
authors.forename: H S
authors.initials: HS
authors.lastname: Fox
authors.forename: G R
authors.initials: GR
authors.lastname: Martin
authors.forename: M F
authors.initials: MF
authors.lastname: Lyon
authors.forename: B
authors.initials: B
authors.lastname: Herrmann
authors.forename: A M
authors.initials: AM
authors.lastname: Frischauf
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: L M
authors.initials: LM
authors.lastname: Silver
mesh_terms: D000483:Alleles; D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D008040:Genetic Linkage; D006183:H-2 Antigens / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D013489:Suppression, Genetic
chemical_list: D004274:DNA, Recombinant; D006183:H-2 Antigens; D004247:DNA; D004262:DNA Restriction Enzymes