Herpes virus infection prevalence in regular haemodialysis patients--a comparative evaluation of complement fixation, indirect immunofluorescence and Elisa tests.
The presence and titres of specific serum IgG and IgM antibodies to cytomegalovirus, herpes simplex virus and varicella-zoster virus were evaluated in 50 haemodialysis patients by complement fixation, immunofluorescence and Elisa tests. A second serum sample was tested in 24 patients after four weeks. Specific serum IgG antibodies to Epstein-Barr virus were also measured by immunofluorescence in 26 patients. By immunofluorescence and Elisa tests, the prevalence of cytomegalovirus, herpes simplex virus and Epstein-Barr virus infection is approximately 100 per cent, and varicella-zoster virus 60 per cent. High titres of IgG specific antibodies found by Elisa tests, detection of IgM antibodies in 16-18 per cent of patients and sero-conversion in 25 per cent of patients, suggests continuous antigenic stimulation.
Scolari
F
F
Manca
N
N
Sandrini
S
S
Cristinelli
L
L
Prati
E
E
Setti
G
G
Savoldi
S
S
Turano
A
A
Maiorca
R
R
D000328:Adult; D000368:Aged; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000209:etiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects*