Uvr-independent repair of 8-methoxypsoralen crosslinks in Escherichia coli: evidence for a recombinational process.
On the basis of survival data, repair of 8-methoxypsoralen DNA crosslinks in Escherichia coli strains lacking a functional uvrABC endonuclease, is shown to require the products of the recA, recB, recF and recN genes. Bacteria, grown under conditions where most cells contain only a single genome, show no evidence of crosslink repair. Similarly, bacteriophage lambda shows evidence of crosslink repair only in SOS-induced cells, and only at multiplicities of infection greater than 1. The requirement for rec+ genes may be partly ascribed to the need for a functional SOS response, but taken together, the results suggest a recombinational step involving a homologous region of DNA may occur during uvr-independent crosslink repair.
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D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D010582:Bacteriophage lambda / Q000235:genetics; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D011088:DNA Ligases / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism / Q000528:radiation effects; D004279:DNA, Viral / Q000378:metabolism / Q000528:radiation effects; D004706:Endodeoxyribonucleases; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000378:metabolism; D008730:Methoxsalen / Q000494:pharmacology*; D011118:Polynucleotide Ligases / Q000378:metabolism*; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D004279:DNA, Viral; D029968:Escherichia coli Proteins; D011938:Rec A Recombinases; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C038225:recE protein, E coli; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli; D004262:DNA Restriction Enzymes; C036385:endodeoxyribonuclease uvrABC; D011088:DNA Ligases; D011118:Polynucleotide Ligases; D008730:Methoxsalen