title: The sequence of rat leukosialin (W3/13 antigen) reveals a molecule with O-linked glycosylation of one third of its extracellular amino acids.
abstract: Leukosialin is one of the major glycoproteins of thymocytes and T lymphocytes and is notable for a very high content of O-linked carbohydrate structures. The full protein sequence for rat leukosialin as translated from cDNA clones is now reported. The molecule contains 371 amino acids with 224 residues outside the cell, one transmembrane sequence and 124 cytoplasmic residues. Data from the peptide sequence and carbohydrate composition suggest that one in three of the extracellular amino acids may be O-glycosylated with no N-linked glycosylation sites. The cDNA sequence contained a CpG rich region in the 3' coding sequence and a large 3' non-coding region which included tandem repeats of the sequence GGAT.
authors.affiliation: MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, UK.
authors.forename: N
authors.initials: N
authors.lastname: Killeen
authors.forename: A N
authors.initials: AN
authors.lastname: Barclay
authors.forename: A C
authors.initials: AC
authors.lastname: Willis
authors.forename: A F
authors.initials: AF
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001483:Base Sequence; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D051917:Leukosialin; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D051381:Rats; D012795:Sialoglycoproteins / Q000235:genetics* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D051917:Leukosialin; D012795:Sialoglycoproteins; C497864:Spn protein, rat; D004247:DNA