Biosynthesis of bloodgroup I and i antigens. A sensitive and specific assay of UDP-GlcNAc:beta-galactoside beta 1----3-N-acetylglucosaminyltransferase activity in hematopoietic cells by HPLC.
A sensitive HPLC method for the assay of UDP-GlcNAc:beta-galactoside beta 1----3-N-acetylglucosaminyltransferase activity was developed. Using lactose as an acceptor, the formation of the product GlcNAc beta 1----3Gal beta 1----4Glc can be determined without interference by substrates resulting from enzymatic and chemical breakdown of the donor substrate UDP-GlcNAc. The method is very specific since products of other transferase reactions, which potentially may be formed in the incubations in vitro, elute at positions different from that of GlcNAc beta 1----3Gal beta 1----4Glc. By use of this assay method it could be demonstrated that normal and malignant hematopoietic cells and cell-lines, with the exception of erythrocytes and reticulocytes, contain beta 1----3-N-acetylglucosaminyltransferase activity.
Koenderman
A H
AH
Department of Medical Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
Loppen
P L
PL
Marinus
L A
LA
van den Eijnden
D H
DH
D001789:Blood Group Antigens; D001853:Bone Marrow / Q000201:enzymology*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D007048:I Blood-Group System; D007519:Isoantigens; D017351:N-Acetylglucosaminyltransferases
D001789:Blood Group Antigens; D007048:I Blood-Group System; D007519:Isoantigens; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase