title: Retrovirus-mediated immunosuppression. II. FeLV-UV alters in vitro murine T lymphocyte behavior by reversibly impairing lymphokine secretion.
abstract: Murine splenocytes and cloned murine T cells were used to study the in vitro immunosuppressive effects of UV-inactivated feline leukemia virus (FeLV-UV) on lymphokine secretion. FeLV-UV can significantly depress the accumulation of IL 2 in cultures of Con A-stimulated C57BL/6 splenocytes and in cultures containing the alloreactive helper T cell clone B6D/2-2m plus Con A. Inhibition of lymphokine accumulation in these cultures could not be attributed to absorption or inactivation of IL 2 by the FeLV-UV or to the FeLV-UV-induced production of substances which interfere with the IL 2 bioassay. Thus, FeLV-UV appears to block production and/or secretion of IL 2 by a direct inhibitory effect on IL 2-secreting murine T lymphocytes. Additional studies indicate that FeLV-UV impairs IL 2 production only if added very soon after lymphocyte contact with lymphokine-inducing agents and that IL 2 secretion resumes when FeLV-UV is removed from the culture. FeLV-UV also impairs accumulation of MAF (interferon-gamma?) in cultures of Con A-stimulated C57BL/6 splenocytes and in cultures containing the alloreactive cytotoxic T lymphocyte clone B6D/2-7c plus Con A. The latter observation again suggests that FeLV-UV impairs lymphokine secretion by a direct effect on lymphokine-producing T lymphocytes. Furthermore, it suggests that FeLV-UV does not selectively impair production of IL 2 nor does it have selective inhibitory effects on helper T cells. Rather, FeLV-UV appears to have a general inhibitory effect on lymphokine production by T lymphocytes. Finally, concentrations of FeLV-UV which suppress MAF production by the CTL clone have little influence on the cytolysis mediated by the same cloned T cell population. Thus, the immunosuppressive influence of FeLV-UV is selective for phenomena associated with induction of new T lymphocyte functions, such as lymphokine secretion, and spares other immune functions already expressed by the same cells.
authors.forename: C G
authors.initials: CG
authors.lastname: Orosz
authors.forename: N E
authors.initials: NE
authors.lastname: Zinn
authors.forename: R G
authors.initials: RG
authors.lastname: Olsen
authors.forename: L E
authors.initials: LE
authors.lastname: Mathes
mesh_terms: D000818:Animals; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D005260:Female; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000378:metabolism; D005256:Leukemia Virus, Feline / Q000502:physiology* / Q000528:radiation effects; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D016215:Macrophage-Activating Factors; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D007376:Interleukin-2; D008222:Lymphokines; D016215:Macrophage-Activating Factors