DNA sequence of the leftward junction in the adenovirus-simian virus 40 hybrid Ad2+D2 and determination of the structure of the D2-T antigen.
The nucleotide sequence of the junction between the simian virus 40 early region and the adenovirus type 2 late region L4 in the hybrid virus Ad2+D2 was determined. The deduced amino acid sequence suggests that the D2-T antigen is a chimeric protein sharing 594 amino acids with the C-terminal end of the simian virus 40 T antigen and 104 amino acids with the N terminus of the adenovirus type 2 33,000-molecular-weight protein. The predicted structure of the D2-T antigen was confirmed by an immunoprecipitation analysis.
Baumann
E A
EA
Baur
C P
CP
Baack
M
M
Beck
S
S
D000256:Adenoviridae / Q000235:genetics*; D000595:Amino Acid Sequence; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D006824:Hybridization, Genetic; D013539:Simian virus 40 / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis*
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D014764:Viral Proteins