Rapid method for the isolation of bacteriophages from lysogens.
A rapid method for the isolation of bacteriophages from lysogens is described. Phages are induced in broth medium containing mitomycin C, which is then replicated onto agar medium. Molten medium containing indicator strains is then poured in these plates. Bacterial lysis is subsequently detected with tetrazolium-containing broth.
Parisi
J T
JT
School of Medicine, Department of Microbiology, University of Missouri-Columbia 65212.
Meng
L
L
D000362:Agar; D001435:Bacteriophages / Q000302:isolation & purification*; D003470:Culture Media; D008242:Lysogeny; D013204:Staphylococcus Phages / Q000302:isolation & purification
D003470:Culture Media; D000362:Agar