title: On the specificity of cytochrome c synthetase in recognition of the amino acid sequence of apocytochrome c.
abstract: Two forms of yeast cytochrome c synthetases with different specificities were resolved, one (synthetase I), solubilized from mitochondria or the cell debris with Triton X-100, recognizing not horse apocytochrome c but yeast apo-iso-1-cytochrome c as a substrate and the other (synthetase II) still bound with the particulate fraction from mitochondria after treatment with Triton, recognizing both horse and yeast apocytochromes c. The activity with labeled yeast apo-iso-1-cytochrome c as a substrate of cytochrome c synthetase I can be quantitatively inhibited by nonlabeled Candida krusei apocytochrome c and partially by nonlabeled tuna apocytochrome c but not by nonlabeled horse apocytochrome c indicating a specific amino acid sequence being recognized. However, an enzyme similarly solubilized from beef heart mitochondria recognized both horse apocytochrome c and yeast apo-iso-1-cytochrome c for attachment of heme. In view of the fact that the yeast synthetase II and the beef synthetase can both utilize either horse apocytochrome c or yeast apo-iso-1-cytochrome c as substrates, we suggest that these enzymes may also be involved in biosynthesis of cytochrome c1, that is, the ability to attach heme to apocytochrome c and apocytochrome c1 may have been conserved in eucaryotic cells, and that both synthetases may therefore be homologous.
authors.forename: C
authors.initials: C
authors.lastname: Visco
authors.forename: H
authors.initials: H
authors.lastname: Taniuchi
authors.forename: B S
authors.initials: BS
authors.lastname: Berlett
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001059:Apoproteins / Q000378:metabolism*; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism*; D045304:Cytochromes c; D008190:Lyases; D008928:Mitochondria / Q000201:enzymology; D008929:Mitochondria, Heart / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013045:Species Specificity; D013379:Substrate Specificity; D014166:Transferases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001059:Apoproteins; D003574:Cytochrome c Group; D045304:Cytochromes c; D014166:Transferases; D008190:Lyases; C031581:cytochrome C synthetase