title: Cyclohexyladenosine binding in rat striatum.
abstract: N6-Cyclohexyl-[3H]adenosine [( 3H]CHA) binds specifically to rat brain membranes prepared from the caudate-putamen complex with a Kd value of 2.50 +/- 0.39 nM and Bmax of 458 +/- 51 fmol/mg protein. Lesioning the nigrostriatal dopaminergic pathway using 6-hydroxydopamine failed to alter [3H]CHA binding characteristics. Intrastriatal kainate lesions reduced the binding capacity of [3H]CHA by 28% though this was not statistically significant (0.1 less than P greater than 0.05). In kainate-lesioned striata, however, 2-deoxyglucose uptake was reduced by only 39%.
authors.forename: H G
authors.initials: HG
authors.lastname: Lloyd
authors.forename: T W
authors.initials: TW
authors.lastname: Stone
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D002421:Caudate Nucleus / Q000032:analysis / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism*; D006892:Hydroxydopamines / Q000494:pharmacology; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D016627:Oxidopamine; D011699:Putamen / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000032:analysis; D011983:Receptors, Purinergic
chemical_list: D006892:Hydroxydopamines; D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; C027513:N(6)-cyclohexyladenosine; D016627:Oxidopamine; D000241:Adenosine; D007608:Kainic Acid