Identification of subtypes of [3H]prazosin-labelled alpha 1 receptor binding sites in rat brain.
Detailed antagonist competition curves for [3H]prazosin-labelled binding sites in rat cerebral cortex membranes reveal anomalous binding characteristics. Dihydroergocryptine and indoramine compete in a steep, monophasic manner while WB4101 and phentolamine exhibit shallow competition curves. Computer-assisted analysis of binding data indicate that both WB4101 and phentolamine discriminate identical subpopulations of [3H]prazosin binding sites, which each comprise approximately 50% of specific [3H]prazosin binding. These data suggest the presence of subtypes of [3H]prazosin-labelled alpha 1 adrenergic receptors in rat cerebral cortex.
Morrow
A L
AL
Battaglia
G
G
Norman
A B
AB
Creese
I
I
D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry; D004146:Dioxanes / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010646:Phentolamine / Q000378:metabolism; D011224:Prazosin / Q000378:metabolism*; D011799:Quinazolines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000032:analysis*; D014316:Tritium
D004146:Dioxanes; D011799:Quinazolines; D011942:Receptors, Adrenergic, alpha; D014316:Tritium; C010654:(2-(2',6'-dimethoxy)phenoxyethylamino)methylbenzo-1,4-dioxane; D011224:Prazosin; D010646:Phentolamine