[Polymorphism of membrane proteinases from mitochondria].
Using native polyacrylamide gel electrophoresis in the absence of sodium dodecyl sulfate, the detergent extracts of sonic submitochondrial particles (SMP) were separated into three protein fractions capable of accomplishing the proteolysis of cytochrome c and three other fractions catalyzing the hydrolysis of N-a-benzoyl-L-arginine-p-nitroanilide (BAPA) and N-a-benzoyl-L-arginine-B-naphthylamide (BANA). The fractions isolated from the gel were subjected to a thorough anaylsis. Cytochrome c hydrolases were shown to have identical molecular weights (17000) but different isoelectric points (4.0, 4.2 and 4.4). The total cytochrome c hydrolase activity of these enzymes was inhibited by phenylmethylsulfonylfluoride but was insensitive to ethylenediaminetetraacetate and o-phenanthroline. Three BANA (BAPA) hydrolases have identical Mr values (approximately 17500) but different pI values (4.2, 4.3 and 4.7). Apart from the indicated hydrolases, the detergent extracts of SMP were shown to contain minor components with identical activities distinguished by the tightness of binding to the inner mitochondrial membrane, Mr and sensitivity to proteinase inhibitors. The observed phenomenon is considered to be due to the polymorphism of proteinases coupled with the inner mitochondrial membrane.
Zubatov
A S
AS
Mikhailova
A E
AE
Luzikov
V N
VN
D001586:Benzoylarginine Nitroanilide / Q000378:metabolism; D001587:Benzoylarginine-2-Naphthylamide / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism*; D011110:Polymorphism, Genetic; D011480:Protease Inhibitors / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
D003574:Cytochrome c Group; D011480:Protease Inhibitors; D001587:Benzoylarginine-2-Naphthylamide; D001586:Benzoylarginine Nitroanilide; D010447:Peptide Hydrolases
Polimorfizm membrannyx proteinaz mitokhondriĭ.