title: Study of beta-glucosidase from Helix pomatia by active site-directed inhibitors.
abstract: This work describes the purification of a beta-glucosidase (beta-D-glucoside-glucohydrolase EC 3.2.1.21) from the digestive juice of Helix pomatia and the study of the enzyme's active site by using different reversible and irreversible inhibitors. The catalytic constants of arylglycosides and their pH-dependent variations have also been determined. The inhibition studies demonstrate that conduritol epoxides are irreversible inhibitors of beta-glucosidase from the digestive juice of H. pomatia, and that nojirimicin shows tight binding with glucosidase: the formation and dissociation of the enzyme-inhibitor complex (dissociation constant 1.1 mumol/1) required several minutes.
authors.affiliation: Laboratoire de Biochimie, Hôpital Tenon, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Donsimoni
authors.forename: G
authors.initials: G
authors.lastname: Legler
authors.forename: R
authors.initials: R
authors.lastname: Bourbouze
authors.forename: P
authors.initials: P
authors.lastname: Lalegerie
mesh_terms: D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives; D000818:Animals; D005944:Glucosamine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D005959:Glucosidases / Q000378:metabolism*; D006372:Helix, Snails / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007294:Inositol / Q000031:analogs & derivatives* / Q000494:pharmacology; D007700:Kinetics; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D017485:1-Deoxynojirimycin; D007294:Inositol; D005959:Glucosidases; D001617:beta-Glucosidase; D005944:Glucosamine; C011423:conduritol epoxide; C013626:nojirimycin