Detection of BK virus antibodies measured by enzyme-linked immunosorbent assay (ELISA) and two haemagglutination inhibition methods: a comparative study.
We developed a solid phase enzyme-linked immunosorbent assay (ELISA) for detecting IgG antibodies to BK virus (BKV). This test was compared to two haemagglutination inhibition methods, one using receptor-destroying enzyme and the other NaIO4 to remove nonspecific inhibitors. The results show that this single dilution ELISA is a sensitive and specific test. The inherent advantages of this method make it an attractive alternative for the seroepidemiology of BKV.
Flaegstad
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T
Traavik
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D000914:Antibodies, Viral / Q000032:analysis*; D001739:BK Virus / Q000276:immunology*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D011120:Polyomavirus / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G