Carcinogen-induced insertion mutations in E. coli.
Mutagenic effects of AAF have been examined by transforming competent E. coli cells with plasmid DNA which were regionally-modified with this carcinogen. Transposition of IS5 from chromosomal DNA to target sequences in the AAF-modified region was detected in six plasmids obtained from wild type and recA strains. A single GC addition was identified in an additional plasmid; no deletions were detected. The observed frequency of IS5 insertion, 2.5 X 10(-3), exceeds that reported for naturally-occurring IS5 transposition in E.coli. These results suggest that the frequency of recombinational events in E.coli can be increased by the presence of a DNA-carcinogen adduct.
Takeshita
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M
Van der Keyl
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H
Grollman
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AP
D000099:Acetoxyacetylaminofluorene / Q000494:pharmacology; D001483:Base Sequence; D002273:Carcinogens / Q000494:pharmacology*; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements / Q000187:drug effects*; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D009154:Mutation; D014169:Transformation, Bacterial
D002273:Carcinogens; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D000099:Acetoxyacetylaminofluorene; D004262:DNA Restriction Enzymes