Wound-induced proteinase inhibitors from tomato leaves. I. The cDNA-deduced primary structure of pre-inhibitor I and its post-translational processing.
A cDNA containing the coding region for the complete amino acid sequence of wound-induced proteinase Inhibitor I from tomato leaves was constructed in the plasmid pUC9 and characterized. The open reading frame codes for a protein of 111 amino acids. This deduced amino acid sequence revealed the presence of a 42-amino acid N-terminal sequence that is not found in the native protein. This sequence appears to contain a 23-amino acid segment typical of a signal sequence followed by a 19-amino acid sequence containing 9 charged amino acids. The 42-amino acid sequence is apparently lost during maturation to the native Inhibitor I and represents 38% of the translated protein. The Inhibitor I amino acid sequence contains 71% identity with potato tuber Inhibitor I sequence and 35% identity with an inhibitor from the leech.
Graham
J S
JS
Pearce
G
G
Merryweather
J
J
Titani
K
K
Ericsson
L
L
Ryan
C A
CA
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D010935:Plant Diseases; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D011480:Protease Inhibitors / Q000032:analysis* / Q000235:genetics; D011498:Protein Precursors / Q000032:analysis* / Q000235:genetics; D011499:Protein Processing, Post-Translational
D010940:Plant Proteins; D011480:Protease Inhibitors; D011498:Protein Precursors; C023578:proteinase inhibitor I (plants); D004247:DNA; D004262:DNA Restriction Enzymes