A spectrophotometric method for N-nicotinoyl-tryptamine (tryptamide) assay in the blood serum of rats.
A spectrophotometric method for N-nicotinoyl-tryptamine (tryptamide) (TR) assay in the blood serum of rats has been elaborated. Sensitivity of the method is approx. 1 microgram of TR/ml of blood serum, recovery approx. 76%. Using this method a preliminary pharmacokinetic study of TR was carried out in rats after ip or po administration of different doses of TR. It was found that the total amount of TR, expressed as corresponding AUC values, was nearly the same after both routes of administration of the same dose of TR. In addition, an effect of two solubilizing agents, 1% Tween 80 and gum arabic on TR (free base or hydrochloride) absorption from gastrointestinal tract of rats into general circulation has been investigated. Results of this study indicated the best absorption of TR when the substance, was given to rats as a hydrochloride suspended in 1% Tween 80 solution.
Danek
L
L
Institute of Pharmacology, Polish Academy of Sciences, Kraków.
Rurak
A
A
Melzacka
M
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D008297:Male; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry; D014363:Tryptamines / Q000097:blood* / Q000493:pharmacokinetics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D014363:Tryptamines; D009536:Niacinamide; C057564:tryptamide