Experimental parainfluenza virus infection in mice: growth and spread of a highly pathogenic variant of parainfluenza 3 virus in the mouse brain.
We had previously showed that following intracerebral inoculation of newborn mice, the 910 N and M strains of bovine parainfluenza 3 virus induce a non-lethal hydrocephalus and a lethal disease with marked thymic and splenic atrophy, respectively. Moreover, only the M virus was lethal for 2-week-old mice. In the present study, we demonstrate that the M virus multiplies and spreads in the mouse brain invading the thalamus, hypothalamus and brain stem beyond the ependyma whereas the 910 N virus causes only slight ependymitis. This growth and spread of M virus was blocked by passive immunization 3 days after infection. Mouse embryo brain cell cultures were infected with M and 910 N viruses, about 50 per cent became antigen-positive for M whereas only a small proportion of cells were positive for the 910 N virus. However, the latter did produce higher yeilds of infectious virus than M.
Shibuta
H
H
Kanda
T
T
Nozawa
A
A
Sato
S
S
Kumanishi
T
T
D000818:Animals; D000831:Animals, Newborn; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000382:microbiology; D006849:Hydrocephalus / Q000209:etiology* / Q000382:microbiology; D007116:Immunization, Passive; D007158:Immunologic Techniques; D051379:Mice; D010224:Parainfluenza Virus 3, Human / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D018184:Paramyxoviridae Infections / Q000209:etiology* / Q000382:microbiology; D018108:Respirovirus / Q000472:pathogenicity*
D000956:Antigens, Viral