Suppression by phenytoin of convulsant-induced afterdischarges at presynaptic nerve terminals.
The mechanisms underlying the induction of afterdischarges at presynaptic nerve terminals by convulsant aminopyridines and their suppression by the anticonvulsant drug phenytoin were studied at the frog neuromuscular preparation. Addition of aminopyridine to the perfusing solution induced the appearance of afterdischarges in motor nerve fibres following their primary response to a single nerve stimulus. The afterdischarges seemed to originate at or near the nerve terminals and to propagate both antidromically and orthodromically. The latter resulted in repetitive activation of the neuromuscular synapse. Focal recordings of nerve terminal potentials suggested that aminopyridines may induce afterdischarges by slowing spike repolarization and thereby producing a prolonged depolarization of nerve terminals. Phenytoin suppressed the aminopyridine-induced afterdischarges and the resultant repetitive excitation of the postsynaptic muscle fibres. This effect of phenytoin was associated with a depression of the action potential at the motor nerve terminals but not at their parent axons. These results single the presynaptic nerve terminals as preferential sites for convulsant and anticonvulsant actions.
David
G
G
Selzer
M E
ME
Yaari
Y
Y
D015761:4-Aminopyridine; D000200:Action Potentials / Q000187:drug effects; D000631:Aminopyridines / Q000037:antagonists & inhibitors*; D000818:Animals; D002118:Calcium / Q000502:physiology; D066298:In Vitro Techniques; D009469:Neuromuscular Junction / Q000187:drug effects*; D010672:Phenytoin / Q000494:pharmacology*; D011895:Rana ridibunda; D009435:Synaptic Transmission / Q000187:drug effects*
D000631:Aminopyridines; D010672:Phenytoin; D015761:4-Aminopyridine; C038615:2,3-diaminopyridine; D002118:Calcium