title: Acute changes in blood glucose concentration do not promote thrombin generation or fibrin breakdown in type 1 diabetes.
abstract: To investigate the effect of blood glucose concentration on thrombin generation and fibrinolytic activity, six Type 1 patients had the blood glucose concentration maintained for 1 h at 5, 15, and 25 mmol l-1, and 8 patients underwent hypoglycaemia of 20 min duration after the blood glucose had been kept at 8 mmol l-1 for 1 h. During hyperglycaemia plasminogen activator activity rose from 214 (11-625) (median, range) to 478 (18-772) units (p less than 0.05) at a blood glucose of 5 mmol l-1 and to 511 (89-816) (p less than 0.05) and 535 (33-976) (p less than 0.05) units at a blood glucose of 15 and 25 mmol l-1, respectively. Cross-linked fibrin degradation products (FDP) were 45 and 53 micrograms l-1 at a blood glucose of 5 mmol l-1 and remained unchanged at higher glucose levels. Fibrinopeptide A was 1.3 (0.6-2.8) nmol l-1 at a blood glucose of 5 mmol l-1, and remained unchanged with hyperglycaemia, being 1.3 (0.9-1.3) nmol l-1 after 1h at 25 mmol l-1. During hypoglycaemia, plasminogen activator activity rose from 155 to 745 units (p less than 0.05) while both fibrinopeptide A and cross-linked FDP remained unchanged. The results indicate that acute fluctuations in blood glucose concentration do not lead to thrombin generation. Additionally, increased fibrinolytic activity measured in vitro is not associated with an increase in cross-linked FDP. This suggests that short-term hyper- and hypoglycaemia do not affect the end-products of the coagulation and fibrinolytic pathways.
authors.affiliation: University Department of Medicine, Leeds, UK.
authors.forename: P J
authors.initials: PJ
authors.lastname: Grant
authors.forename: M H
authors.initials: MH
authors.lastname: Stickland
authors.forename: P G
authors.initials: PG
authors.lastname: Wiles
authors.forename: P J
authors.initials: PJ
authors.lastname: Gaffney
authors.forename: J A
authors.initials: JA
authors.lastname: Davies
authors.forename: C R
authors.initials: CR
authors.lastname: Prentice
mesh_terms: D001786:Blood Glucose / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005337:Fibrin / Q000378:metabolism*; D005344:Fibrinopeptide A / Q000032:analysis; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D006943:Hyperglycemia / Q000097:blood; D007003:Hypoglycemia / Q000097:blood; D008297:Male; D010960:Plasminogen Activators / Q000032:analysis; D013917:Thrombin / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D005337:Fibrin; D005344:Fibrinopeptide A; D006442:Glycated Hemoglobin; D010960:Plasminogen Activators; D013917:Thrombin