Establishment of the Amsalem T-cell line from a patient with acute lymphoblastic leukemia. Expression of E-receptor-associated antigens in cells incapable of forming E-rosettes.
A new T-cell line (Amsalem) was established from the peripheral blood of a patient with pre-T leukemia. Amsalem cells are unique in that they possess antigenic determinants associated with the E-receptor, yet fail to form rosettes with sheep red blood cells (SRBC). Amsalem cells were found to possess morphological and cytochemical features characteristic of T-lymphocytes, and were sensitive to the cytotoxic effect of rabbit antisera specific for T-cell antigens. In immunofluorescent tests with monoclonal antibodies, Amsalem cells showed a strong reactivity with the OKT-11A and A-22 antibodies, specific for the E-receptor. The cells were reactive with OKT-4 and showed a very weak reactivity with OKT-6 and OKT-8. No reactivity was found with the OKT-3, Leu 7, Leu 11, and OKM1 antibodies. Amsalem cells failed to form rosettes with SRBC; however, mouse anti-Amsalem serum inhibited the formation of E-rosettes. It is concluded that the Amsalem cell line is a line of pre-T leukemia cells characterized by a dissociation between its inability to form E-rosettes and the presence of antigenic constituents of the E-receptor.
Ben Bassat
H
H
Rabinowitz
R
R
Polliack
A
A
Hadar
R
R
Schlesinger
M
M
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D002460:Cell Line; D002648:Child; D005260:Female; D005455:Fluorescent Antibody Technique; D006511:Hepatitis B Antigens / Q000032:analysis*; D006513:Hepatitis B e Antigens / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000276:immunology; D006651:Histocytochemistry; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008855:Microscopy, Electron, Scanning; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011951:Receptors, Complement / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis; D011991:Receptors, Virus / Q000032:analysis; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology / Q000473:pathology
D000911:Antibodies, Monoclonal; D006511:Hepatitis B Antigens; D006513:Hepatitis B e Antigens; D011947:Receptors, Antigen, B-Cell; D011951:Receptors, Complement; D011961:Receptors, Fc; D011991:Receptors, Virus