Alloantigen presenting capacity of human decidual tissue.
The capacity of first trimester human decidua-derived cells to serve as accessory cells for the presentation of alloantigens to unprimed T lymphocytes was assessed using a mixed lymphocyte culture (MLC) between accessory cell-depleted responder and stimulator peripheral blood lymphocytes (d PBL). In all of the seven experiments performed decidua-derived cells achieved significant reconstitution of the lymphoproliferative response between autologous responder and stimulator dPBL. Reconstitution indices (RIs) ranged between 3.1 and 22.2 and were significant in all cases. In six out of the seven experiments the level of lymphoproliferation exceeded that between untreated responder PBLs co-cultured with stimulator d PBL. Substitution of recombinant interleukin 1 (rIL-1) alpha or beta for the decidua-derived cells resulted in significantly lower RIs than those achieved by the decidua-derived cells. These findings suggest that decidua-derived cells can serve as antigen presenting cells for the presentation of membrane-bound alloantigens in a primary lymphoproliferative response. This may have implications for maternal recognition of fetal antigens during first pregnancy.
Dorman
P J
PJ
Department of Anatomy, Medical School, University of Newcastle upon Tyne, U.K.
Searle
R F
RF
D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface; D003656:Decidua / Q000276:immunology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D007375:Interleukin-1; D007519:Isoantigens