Con A-propagated, auto-reactive T cell clones that secrete factors promoting high IgA responses.
Four BALB/c T cell clones from among a set propagated in the presence of concanavalin A (Con A) were selected on the basis of their ability to produce supernatant factors promoting high IgA plaque-forming cell (PFC) responses by 2,4,6-trinitrophenyl-conjugated keyhole limpet hemocyanin (TNP-KLH)-primed splenic B cells in the presence of TNP-SRBC. Such clones could be derived from cultures containing T cells not only from gut-associated lymphoid tissue, but also from the spleen. The selected clones all proliferated well in the presence of syngeneic, irradiated APC without either Con A or exogenous IL-2, but required both APC and Con A to produce helper factors. Factors from three of the clones helped B cells both to proliferate and to differentiate into IgM, IgG and IgA PFC. Factors from the fourth clone helped B cells differentiate into IgA and IgG PFC and may have promoted switching to these isotypes but did not support either B cell proliferation or generation of IgM PFC. Cross-linking of B cell receptors for antigen was not required for the response to the helper factors since TNP-SRBC were unnecessary and high concentrations of them were actually inhibitory.
Maghazachi
A A
AA
Department of Pathology, New York University Medical Center.
Phillips-Quagliata
J M
JM
D000818:Animals; D015703:Antigens, CD; D015478:Antigens, Heterophile / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002474:Cell-Free System; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D003208:Concanavalin A; D004247:DNA / Q000096:biosynthesis; D006462:Hemolytic Plaque Technique; D007070:Immunoglobulin A / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc / Q000032:analysis; D012756:Sheep; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000378:metabolism; D014301:Trinitrobenzenes / Q000276:immunology
D015703:Antigens, CD; D015478:Antigens, Heterophile; C036832:Fc(alpha) receptor; D007070:Immunoglobulin A; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D011961:Receptors, Fc; D014301:Trinitrobenzenes; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D003208:Concanavalin A; D004247:DNA