Detection of sendai virus protein by reversed-phase high-performance liquid chromatography combined with immuno-chromatography.
Reversed-phase high-performance liquid chromatography (RP-HPLC) was applied to the detection of Sendai virus F2 protein. Immunoaffinity chromatography was used prior to RP-HPLC to increase the specificity. With this combined method, the presence of F2 protein in complex solutions could be demonstrated within 150 min. Using RP-HPLC, F2 protein could be detected in amounts down to 20 ng, which could be reduced to 1 ng by application of a microbore column.
van der Zee
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Welling
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GW
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007120:Immunochemistry; D010222:Parainfluenza Virus 1, Human / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*
D014764:Viral Proteins