title: Isolation, characterization, and regulation of the prolactin receptor.
abstract: The prolactin, or lactogenic hormone, receptor has been purified (approximately 80%) from lactating mouse liver and human term placenta by the nondenaturing zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate and a prolactin affinity column. The isolated "core-binding unit" has a molecular weight of 37,000 +/- 2,000 daltons. It retains the specificity for lactogenic hormones and binds prolactin with an affinity (Ka = 2 to 6 X 10(9) M-1) similar to that of the receptor as it occurs in its membranous environment (Ka = 3 to 5 X 10(9) M-1). Whether this "core-binding unit" exists on the cell surface in a cryptic or active form is influenced greatly by its association with other membrane proteins and the concentration of phosphatidylcholine within its local membranous environment.
authors.forename: B K
authors.initials: BK
authors.lastname: Vonderhaar
authors.forename: A
authors.initials: A
authors.lastname: Bhattacharya
authors.forename: T
authors.initials: T
authors.lastname: Alhadi
authors.forename: D S
authors.initials: DS
authors.lastname: Liscia
authors.forename: E M
authors.initials: EM
authors.lastname: Andrew
authors.forename: J K
authors.initials: JK
authors.lastname: Young
authors.forename: E
authors.initials: E
authors.lastname: Ginsburg
authors.forename: M
authors.initials: M
authors.lastname: Bhattacharjee
authors.forename: T M
authors.initials: TM
authors.lastname: Horn
mesh_terms: D000818:Animals; D002793:Cholic Acids; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D008099:Liver / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008861:Microsomes / Q000032:analysis; D008862:Microsomes, Liver / Q000032:analysis; D008954:Models, Biological; D008970:Molecular Weight; D010743:Phospholipids / Q000378:metabolism; D010920:Placenta / Q000032:analysis; D011388:Prolactin / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism; D011981:Receptors, Prolactin; D012995:Solubility
chemical_list: D002793:Cholic Acids; D010743:Phospholipids; D011956:Receptors, Cell Surface; D011981:Receptors, Prolactin; D003208:Concanavalin A; D011388:Prolactin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate