Early clonality and high-frequency proviral integration into the c-myc locus in AKR leukemias.
Blot hybridization of thymocyte DNA from AKR/J mice was used to detect new proviral junction fragments as markers of clonality at different stages of viral leukemogenesis and to detect DNA rearrangements at the c-myc locus due to proviral insertion. Clonal populations of thymocytes were observed in mink cell focus-forming virus-injected mice as early as 35 days postinjection, at a stage distinguishable from frank leukemia by flow cytometric analysis and transplantation bioassay. Specific proviral integrations in the c-myc locus were detected in 15% of these early clones and in up to 65% of late-developing thymomas and frank leukemias. Thus, in this system c-myc activation appears to be a common mechanism in T-cell leukemogenesis.
O'Donnell
P V
PV
Fleissner
E
E
Lonial
H
H
Koehne
C F
CF
Reicin
A
A
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002999:Clone Cells; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000235:genetics*; D007942:Leukemia, Experimental / Q000235:genetics / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008806:Mice, Inbred AKR; D009857:Oncogenes; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000382:microbiology / Q000473:pathology; D013945:Thymoma / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D013953:Thymus Neoplasms / Q000235:genetics / Q000382:microbiology / Q000473:pathology