Human lens gamma-crystallin sequences are located in the p12-qter region of chromosome 2.
The human gamma-crystallin genes constitute a multigene family whose members are only expressed in the eye lens. The chromosomal location of these sequences has been determined by screening a panel of human/rodent hybrid cell lines containing overlapping subsets of human chromosomes for the presence of human gamma-crystallin sequences. By correlating these genomic hybridization data with the chromosomal constitution of the somatic cell hybrids, all human gamma-crystallin sequences could be assigned to chromosome 2. The use of human/hamster cell hybrids derived from human Burkitt lymphoma cells carrying a reciprocal translocation between human chromosomes 2 and 8, allowed a further localization of the sequences to the region 2p12-qter.
den Dunnen
J T
JT
Jongbloed
R J
RJ
Geurts van Kessel
A H
AH
Schoenmakers
J G
JG
D000818:Animals; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3; D006224:Cricetinae; D003459:Crystallins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009693:Nucleic Acid Hybridization; D051381:Rats
D003459:Crystallins; D004247:DNA; D004262:DNA Restriction Enzymes