title: Phosphorylation of neurofilament proteins by endogenous calcium/calmodulin-dependent protein kinase.
abstract: A protein fraction containing neurofilaments was prepared from rat brain cytosol by differential centrifugation and gel filtration chromatography. These preparations were enriched for a calcium/calmodulin-dependent kinase activity that phosphorylated endogenous neurofilament proteins. The enzyme incorporated approximately 1 mol PO4/mol of each neurofilament triplet polypeptide. These data suggest that a calmodulin-dependent kinase may mediate some of the effects of calcium on cytoskeletal function by phosphorylation of neurofilament proteins.
authors.forename: M L
authors.initials: ML
authors.lastname: Vallano
authors.forename: T M
authors.initials: TM
authors.lastname: Buckholz
authors.forename: R J
authors.initials: RJ
authors.lastname: DeLorenzo
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002147:Calmodulin / Q000302:isolation & purification; D002417:Cattle; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007381:Intermediate Filament Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D016900:Neurofilament Proteins; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism
chemical_list: D002147:Calmodulin; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases