title: Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene.
abstract: We describe the cloning and the DNA sequence of an amber suppressor allele of the Escherichia coli leuX (supP) gene. The suppressor allele codes for a tRNA with anticodon CUA, presumably derived by a single base change from a CAA anticodon. The mature coding sequence of the leuX gene is preceded by a putative Pribnow box sequence (TATAAT) and followed by a termination signal. The sequence of the leuX-coded tRNA is compared with the sequences of the four remaining tRNALeu isoacceptors of E. coli and with two tRNALeu species from bacteriophage T4 and T5. The conserved nucleotides in these seven tRNAs recognized by E. coli leucyl-tRNA synthetase are located mainly in the aminoacyl stem and in the D-stem/loop region.
authors.forename: S
authors.initials: S
authors.lastname: Thorbjarnardóttir
authors.forename: T
authors.initials: T
authors.lastname: Dingermann
authors.forename: T
authors.initials: T
authors.lastname: Rafnar
authors.forename: O S
authors.initials: OS
authors.lastname: Andrésson
authors.forename: D
authors.initials: D
authors.lastname: Söll
authors.forename: G
authors.initials: G
authors.lastname: Eggertsson
mesh_terms: D000926:Anticodon; D010582:Bacteriophage lambda / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D000926:Anticodon; D012346:RNA, Transfer, Amino Acyl; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII