title: Importance of MHC antigen expression on solid tumors in the in vitro interaction with autologous blood lymphocytes.
abstract: In 54 patients with lung and 14 with ovarian carcinomas the quantitative variations of the major histocompatibility complex (MHC)-determined class I and class II antigens of the tumor cells were related to their in vitro interaction with blood lymphocytes, to the lymphoid infiltration of the tumors, and to the metastatic state of the disease. The tumor cell-lymphocyte interaction was measured by the proliferative response in mixed lymphocyte tumor cell culture and by the cytotoxic activity of the lymphocytes. The results showed that (1) none of the 23 tumors from patients with disseminated disease were lysed; (2) class I-negative tumors were not damaged; (3) lymphoid infiltration was present in a higher proportion of class II-positive tumors; and (4) both MHC-positive and -negative tumors were found among the disseminated tumors. The requirement of class I expression in the lytic interaction substantiates our earlier conclusion concerning the cytotoxic T lymphocyte nature of lymphocyte-mediated auto-tumor lysis. The lack of auto-tumor lysis in patients with metastases suggests impairment of lymphocyte function in advanced stages of the disease.
authors.affiliation: Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Vánky
authors.forename: G
authors.initials: G
authors.lastname: Stuber
authors.forename: J
authors.initials: J
authors.lastname: Willems
authors.forename: K
authors.initials: K
authors.lastname: Sjöwall
authors.forename: B
authors.initials: B
authors.lastname: Larsson
authors.forename: K
authors.initials: K
authors.lastname: Böök
authors.forename: T
authors.initials: T
authors.lastname: Ivert
authors.forename: A
authors.initials: A
authors.lastname: Péterffy
authors.forename: E
authors.initials: E
authors.lastname: Klein
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000276:immunology*
chemical_list: D006680:HLA Antigens