A new mutagen-sensitive mutant in Neurospora, mus-16.
A new gene, mus-16, is determined by the nitrogen mustard-sensitive Neurospora mutant of Baker, Parish and Curtis (1984) which is defective in the removal of DNA-DNA and DNA-protein crosslinks. This gene is on the left arm of linkage group V between caf-1 and lys-1. The mus-16(JMB) mutant is sensitive to the alkylating agents methyl methanesulfonate (MMS) [dose reduction factor (drf) 8-10 X], N-methyl-N'-nitro-N-nitrosoguanidine (drf 5-6 X), the amino acid histidine and the drug hydroxyurea. It is not sensitive to ultraviolet-light, gamma-irradiation, or mitomycin C (MMC). It shows normal spontaneous mutation rates but increased induction of mutation by MMS. Homozygous crosses are barren, showing no signs of sporulation. Mitotic spontaneous chromosome instability is increased. The mus-16 mutation is similar to several non-excision repair-defective mutants in Neurospora. Some of these may be defective in repair of alkylation damage. The MMC data supports earlier data that in fungi MMC is incapable of forming DNA-DNA crosslinks.
Inoue
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Department of Regulation Biology, Faculty of Science, Saitama University, Urawa, Japan.
Schroeder
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AL
D002872:Chromosome Deletion; D004260:DNA Repair; D004271:DNA, Fungal; D005800:Genes, Fungal; D008466:Mechlorethamine / Q000494:pharmacology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D011995:Recombination, Genetic; D013172:Spores, Fungal / Q000235:genetics; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D008937:Mitomycins; D009153:Mutagens; D008466:Mechlorethamine; D016685:Mitomycin