title: Calcium transport and catabolism of adenosine triphosphate in the protozoan parasite Giardia lamblia.
abstract: 1. Calcium uptake by washed trophozoites of Giardia lamblia was dependent on inorganic orthophosphate and stimulated by glucose. Uptake was both rapid and substantial: 224 +/- 73 nmoles Ca2+/mg protein/min. 2. Known inhibitors of Ca2+ uptake in mammalian cells also impeded Ca2+ influx into G. lamblia. 3. The inhibitor studies indicated that Ca2+ transport in G. lamblia was an active process. Energy for such a process could be provided by the action of ATPases. 4. Two types of ATPases were found in the parasite; one, a membrane-associated enzyme activated by Ca2+; the other, a soluble, cytosolic enzyme activated by Mg2+. 5. These enzymes differed not only in their intracellular distribution and divalent cation requirements, but also in their sensitivity to calmodulin antagonists. The particulate enzyme was sensitive to these inhibitors whereas the soluble ATPase was not. 6. Our data indicate that Ca2+ transport in G. lamblia is mediated by a membrane-bound, calmodulin-regulated, Ca2+-ATPase.
authors.affiliation: Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda.
authors.forename: M L
authors.initials: ML
authors.lastname: Munoz
authors.forename: C E
authors.initials: CE
authors.lastname: Claggett
authors.forename: E C
authors.initials: EC
authors.lastname: Weinbach
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002147:Calmodulin / Q000037:antagonists & inhibitors; D005872:Giardia / Q000378:metabolism*; D009711:Nucleotides; D012995:Solubility; D013379:Substrate Specificity; D014268:Trifluoperazine / Q000494:pharmacology
chemical_list: D002147:Calmodulin; D009711:Nucleotides; D014268:Trifluoperazine; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium