title: A simple purification method for a glucosyltransferase complex from Streptococcus mutans OMZ 176 with a high yield.
abstract: 1. Glucosyltransferase was purified from Streptococcus mutans OMZ 176 by ammonium sulfate fractionation and gel filtration. 2. The overall purification was about 15,000-fold with a yield of 53%. 3. The purified enzyme appeared to be a multienzyme complex consisting of three different components with molecular weights of 158,000, 153,000 and 149,000, respectively.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Furutani
authors.forename: M
authors.initials: M
authors.lastname: Iwaki
authors.forename: T
authors.initials: T
authors.lastname: Yagi
authors.forename: M
authors.initials: M
authors.lastname: Iida
authors.forename: K
authors.initials: K
authors.lastname: Horiike
authors.forename: M
authors.initials: M
authors.lastname: Nozaki
mesh_terms: D000645:Ammonium Sulfate; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel; D005964:Glucosyltransferases / Q000302:isolation & purification*; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D013291:Streptococcus / Q000201:enzymology*
chemical_list: D011135:Polysaccharides, Bacterial; D005964:Glucosyltransferases; D000645:Ammonium Sulfate