title: Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3") (9).
abstract: The nucleotide sequence of the spc determinant of the Staphylococcus aureus transposon Tn554 has been determined. This gene encodes a spectinomycin adenyltransferase, AAD(9), that mediates resistance to spectinomycin but not to streptomycin. The sequence predicts a 260 amino acid protein of molecular weight 28,943. A spectinomycin-sensitive mutant (spc-1) contains a G----A transition resulting in substitution of threonine (ACA) for alanine (GCA) at residue 165. The predicted amino acid sequence is 36% homologous to that of a widely distributed, gram-negative streptomycin/spectinomycin adenyltransferase, AAD(3") (9), specified by the aadA determinant (Holingshead and Vapnek 1985).
authors.forename: E
authors.initials: E
authors.lastname: Murphy
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D009154:Mutation; D009690:Nucleic Acid Conformation; D009713:Nucleotidyltransferases / Q000235:genetics*; D010957:Plasmids; D011523:Protoplasts / Q000201:enzymology; D000198:Spectinomycin / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D000198:Spectinomycin; D009713:Nucleotidyltransferases; C022682:streptomycin-spectinomycin adenylyltransferase; D004262:DNA Restriction Enzymes