[Partial suppression of rec B rec C mutations in E. coli by plasmid pBR 322 containing a Bacillus subtilis chromosome insertion].
ATP-dependent DNAase genes of Bac. subtilis were originally cloned in E. coli plasmid pBR322. These genes are expressed in rec BC mutant E. coli cells leading to a complete recovery of the enzyme activity. Bac. subtilis enzyme suppresses reparative properties of the rec BC mutant to a considerable extent but is unable to replace functionally the E. coli mutant enzyme in recombination process.
Barabanshchikov
B I
BI
Bashkirov
V I
VI
Gizatullin
F Sh
FSh
Gulitashvili
V Sh
VSh
D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000187:drug effects / Q000201:enzymology*; D003001:Cloning, Molecular / Q000187:drug effects; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics; D009154:Mutation; D010957:Plasmids / Q000187:drug effects; D011995:Recombination, Genetic / Q000187:drug effects; D013489:Suppression, Genetic / Q000187:drug effects
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI
Chastichnaia supressiia mutatsiĭ rec B rec C E. coli plazmidoĭ pBR 322, soderzhashcheĭ vstavku khromosomy Bacillus subtilis.