title: Molecular cloning of engrailed: a gene involved in the development of pattern in Drosophila melanogaster.
abstract: The engrailed gene acts early in Drosophila embryogenesis and plays an essential role in the processes that establish and maintain the repeating segmental pattern. To begin molecular analysis of the role of the engrailed gene in embryonic pattern formation, we used a chromosomal walk to clone genomic sequences that encompass the locus, and have physically mapped the positions of 15 engrailed mutations. The positions of engrailed rearrangement mutations indicate that the engrailed complementation unit includes a minimum of 70 kb. The locus can be divided into two regions. Rearrangement mutations interrupting the centromere proximal 50 kb of the locus result in embryonic lethality while mutants altered in the distal 20 kb of the locus survive to show morphological abnormalities in several adult segments. It appears that long-range cis interactions play a role in the function of the engrailed gene.
authors.forename: J M
authors.initials: JM
authors.lastname: Kuner
authors.forename: M
authors.initials: M
authors.lastname: Nakanishi
authors.forename: Z
authors.initials: Z
authors.lastname: Ali
authors.forename: B
authors.initials: B
authors.lastname: Drees
authors.forename: E
authors.initials: E
authors.lastname: Gustavson
authors.forename: J
authors.initials: J
authors.lastname: Theis
authors.forename: L
authors.initials: L
authors.lastname: Kauvar
authors.forename: T
authors.initials: T
authors.lastname: Kornberg
authors.forename: P H
authors.initials: PH
authors.lastname: O'Farrell
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D005816:Genetic Complementation Test; D009154:Mutation; D014178:Translocation, Genetic
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes