The E. coli multitest: a set of strains to characterize diverse genotoxic effects.
A set of E. coli strains was developed by Toman et al. (1985) to study the effects of chemical and physical agents on forward mutation, homologous recombination and induction of the SOS system. New tester strains have been constructed to improve this test system in order to explore quantitative genotoxicity spectra. Through the use of these strains: (i) SOS induction can be specifically detected without interference from mutagenesis; (ii) SOS-dependent and SOS-independent mutational events can be distinguished; (iii) the sensitivity of the recombination system has been considerably increased.
Tenenbaum
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Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse Belgium.
Quinto
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Faelen
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D000667:Ampicillin / Q000494:pharmacology; D010582:Bacteriophage lambda / Q000235:genetics; D002463:Cell Membrane Permeability; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics*; D005720:Gamma Rays; D005818:Genetic Engineering; D007612:Kanamycin / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D010957:Plasmids; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000378:metabolism; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D008769:Methylnitronitrosoguanidine; D007612:Kanamycin; D000667:Ampicillin