title: Induction of the in vitro anti-HBs response by hepatitis B surface antigen.
abstract: The production in vitro of antibody to hepatitis B surface antigen by peripheral blood mononuclear cells of healthy volunteers was studied after each of the three doses of hepatitis B vaccine. An in vitro hepatitis B surface antigen antibody response was successfully induced in 12% of the specimens taken over a 7 month period. The response to this antigen was induced in additional samples if cells had been treated previously with anti-CD4 and complement or anti-CD8 and complement prior to culture initiation. The addition of interleukin 2 could also induce the formation of antibodies to hepatitis B surface antigen. The results suggest that the antibody response to hepatitis B surface antigen is complex and varies depending on the individual and time of sampling.
authors.affiliation: Department of Microbiology and Immunology, University of Ottawa, Ontario, Canada.
authors.forename: L G
authors.initials: LG
authors.lastname: Filion
authors.forename: R
authors.initials: R
authors.lastname: Saginur
mesh_terms: D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005260:Female; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014761:Viral Hepatitis Vaccines / Q000276:immunology
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007074:Immunoglobulin G; D014761:Viral Hepatitis Vaccines