Ultraviolet radiation augments epidermal beta-adrenergic adenylate cyclase response.
Pig skin was irradiated in vivo with fluorescent sunlamp tubes (peak emission at 305 nm). A significant increase in epidermal beta-adrenergic adenylate cyclase response was observed as early as 12 h following 1-2 minimum erythema doses (MEDs) UVB exposure, which lasted at least 48 h. The augmentation of adenylate cyclase response was relatively specific to the beta-adrenergic system and there was no significant difference in either adenosine- or histamine-adenylate cyclase response of epidermis. The increased beta-adrenergic adenylate cyclase response was less marked at higher doses of UVB exposure (5 MEDs); in the latter condition, a significant reduction in adenosine- or histamine-adenylate cyclase response was observed. There was no significant difference in either low- or high-Km cyclic AMP phosphodiesterase activity between control and UVB-treated skin at 1-2 MEDs. Our data indicate that the epidermal adenylate cyclase responses are affected in vivo by UVB irradiation, which might be a significant regulatory mechanism of epidermal cyclic AMP systems.
Iizuka
H
H
Kajita
S
S
Ohkawara
A
A
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004817:Epidermis / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D004837:Epinephrine / Q000494:pharmacology; D011941:Receptors, Adrenergic / Q000528:radiation effects*; D013552:Swine; D013997:Time Factors; D014466:Ultraviolet Rays
D011941:Receptors, Adrenergic; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000262:Adenylyl Cyclases; D004837:Epinephrine