Isolation and partial characterization of the entire human pro alpha 1(II) collagen gene.
Using a cDNA probe specific for the bovine Type II procollagen, a series of overlapping genomic clones containing 45 kb of contiguous human DNA have been isolated. Sequencing of a 54 bp exon, number 29, provided direct evidence that the recombinant clones bear human Type II collagen sequences. Localization of the 5' and 3' ends of the gene indicated that the human Type II collagen gene is 30 kb in size. This value is significantly higher than that of the homologous avian gene. The segregation of a polymorphic restriction site in informative families conclusively demonstrated that the Type II gene is found in a single copy in the human haploid genome. Finally, sequencing of a triple helical domain exon has confirmed that a rearrangement leading to the fusion of two exons occurred in the pro alpha 1(I) gene, following the divergence of the fibrillar collagens.
Sangiorgi
F O
FO
Benson-Chanda
V
V
de Wet
W J
WJ
Sobel
M E
ME
Tsipouras
P
P
Ramirez
F
F
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006801:Humans; D009693:Nucleic Acid Hybridization; D011347:Procollagen / Q000235:genetics*
D011347:Procollagen; D004247:DNA; D004262:DNA Restriction Enzymes