Biochemical and immunological characterization of mutant L-M cells with altered levels of dibutyryl cyclic AMP-inducible alkaline phosphatase.
Phosphotyrosine-Sepharose 4B was synthesized and used to purify L-cell alkaline phosphatase. Antibodies to this enzyme interacted with the alkaline phosphatase of strains A-1-2 and A-3-3, mutants that express the enzyme constitutively. This and thermal stability studies suggest that these mutants contain the same alkaline phosphatase isozyme as their parent strain.
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Raetz
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D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D005576:Colforsin; D004224:Diterpenes / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D007739:L Cells; D051379:Mice; D009154:Mutation; D013696:Temperature; D013806:Theophylline / Q000494:pharmacology
D004224:Diterpenes; D005576:Colforsin; D003994:Bucladesine; D013806:Theophylline; D000469:Alkaline Phosphatase