title: Heterogeneity of drug-dependent platelet antigens and their antibodies in quinine- and quinidine-induced thrombocytopenia: involvement of glycoproteins Ib, IIb, IIIa, and IX.
abstract: The molecular nature of platelet receptors for quinine- and quinidine-dependent antiplatelet antibodies (Q.Ab and Qd.Ab) was studied by immunoblotting. One Q.Ab caused quinine-dependent IgG binding to platelet proteins with molecular weights (mol wts) of 174 Kd and 93 Kd and another to only a 93-Kd protein. A third Q.Ab caused binding to 174-, 140-, 93-, and 57-Kd proteins, while a fourth Q.Ab and a Qd.Ab caused IgG binding to 174- and 18-Kd proteins. Using platelets from patients with Glanzmann's thrombasthenia or Bernard Soulier syndrome and purified GPIIIa, these proteins were shown to be GPIb, GPIIb, GPIIIa, GPIX, and an unidentified 57-Kd protein missing in Bernard Soulier syndrome. Binding to the 93-Kd protein was independent of the PIA1 antigen. Absorption of one Q.Ab with Glanzmann's thrombasthenia platelets revealed different populations of antibodies with different specificities within the one patient. Thus Q.Ab and Qd.Ab are heterogeneous and may be directed toward different epitopes on major platelet glycoproteins.
authors.affiliation: Department of Medicine, Monash University Medical School, Prahran, Victoria, Australia.
authors.forename: S L
authors.initials: SL
authors.lastname: Pfueller
authors.forename: R A
authors.initials: RA
authors.lastname: Bilston
authors.forename: D
authors.initials: D
authors.lastname: Logan
authors.forename: J M
authors.initials: JM
authors.lastname: Gibson
authors.forename: B G
authors.initials: BG
authors.lastname: Firkin
mesh_terms: D000943:Antigens, Differentiation / Q000032:analysis; D001666:Binding Sites, Antibody / Q000187:drug effects; D001791:Blood Platelet Disorders / Q000097:blood / Q000276:immunology; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007518:Isoantibodies / Q000032:analysis*; D007519:Isoantigens / Q000032:analysis* / Q000276:immunology; D010980:Platelet Membrane Glycoproteins / Q000172:deficiency / Q000302:isolation & purification / Q000378:metabolism*; D011802:Quinidine / Q000009:adverse effects*; D011803:Quinine / Q000009:adverse effects*; D011961:Receptors, Fc / Q000032:analysis; D017452:Receptors, IgG; D013921:Thrombocytopenia / Q000097:blood* / Q000139:chemically induced / Q000276:immunology
chemical_list: D000943:Antigens, Differentiation; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007518:Isoantibodies; D007519:Isoantigens; D010980:Platelet Membrane Glycoproteins; D011961:Receptors, Fc; D017452:Receptors, IgG; D011803:Quinine; D011802:Quinidine