A rapid and sensitive enzyme immunoassay for Cu/Zn superoxide dismutase with polyclonal and monoclonal antibodies.
An enzyme immunoassay for the quantification of human Cu/Zn SOD in serum, urine and erythrocytes was developed applying monoclonal and polyclonal antibodies. The one-step assay is completed within 30 min and enables the detection of 0.3 microgram Cu/Zn SOD per litre. A Cu/Zn SOD concentration of 46 +/- 21.5 micrograms/l and of 1 +/- 0.6 micrograms/mmol creatinine was determined in the serum and the urine, respectively, of healthy individuals. A content of 15 +/- 1.7 ng Cu/Zn SOD was found in 10(6) erythrocytes. Patients with Down's syndrome exhibited a 3.8-fold, a 2-fold and a 1.6-fold higher concentration of Cu/Zn SOD in their serum, urine and erythrocytes.
Porstmann
T
T
Institute of Medical Immunology, Faculty of Medicine, Humboldt University of Berlin, GDR.
Wietschke
R
R
Schmechta
H
H
Grunow
R
R
Porstmann
B
B
Bleiber
R
R
Pergande
M
M
Stachat
S
S
von Baehr
R
R
D000906:Antibodies; D000911:Antibodies, Monoclonal; D002648:Child; D004314:Down Syndrome / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques; D013482:Superoxide Dismutase / Q000032:analysis* / Q000097:blood / Q000652:urine
D000906:Antibodies; D000911:Antibodies, Monoclonal; D013482:Superoxide Dismutase