[Construction and properties of the expression vector based on the temperature-regulated P'R promoter in phage lambda DNA].
Plasmid expression vector using the temperature-regulated promoter P'R of bacteriophage lambda is described. The vector carries a combination of two regions of lambda cI857indgenome, that contain: 1) gene cI and promoter PR, and 2) gene Q and promoter P'R. Transcription or gene Q is initiated at promoter PR, which is controlled by cI857 repressor. The Q gene product acts as a positive regulator of RNA synthesis from P'R. At 37 degrees C, sufficient amounts of protein Q are synthesised to initiate the expression of the cloned gene from P'R. Inactivation of Q gene (by elimination of a single NcoI site) results in the loss of P'R expression activity in the vector. E. coli beta-galactosidase gene (lacZ) and human leukocyte interferon alpha 2 gene (ifn alpha 2) were cloned into a single EcoRI cleavage site under the control of P'R. These constructs express high levels of beta-galactosidase and interferon alpha 2 in E. coli at 37 degrees C.
Petrenko
L A
LA
Gileva
I P
IP
Kravchenko
V V
VV
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D005822:Genetic Vectors; D007370:Interferon Type I / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic
D004279:DNA, Viral; D007370:Interferon Type I
Konstruirovanie i svoĭstva ékspressionnogo vektora na osnove reguliruemogo temperaturoĭ promotora P'R DNK bakteriofaga lambda.