Monoclonal antibodies recognizing early and late antigens of human cytomegalovirus: heterogeneity of polypeptides recognized between virus isolates.
The characteristics of four human cytomegalovirus (HCMV)-specific monoclonal antibodies as assessed by ELISA, immunofluorescence, immunoprecipitation and Western blotting are described. Two antibodies recognized a 67K late polypeptide of HCMV, one recognized 43K and 79K polypeptides present early and late in HCMV-infected cells, and the fourth identified a 72K early nuclear protein of HCMV. The antibodies recognized these antigens in all HCMV isolates tested by immunofluorescence and ELISA, but demonstrated inter-isolate variations in polypeptides recognized by Western blotting.
Rodgers
B C
BC
Mundin
J
J
Sissons
J G
JG
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral