title: Mutational analyses of the Moloney murine sarcoma virus enhancer.
abstract: Retroviral enhancers seem to play an integral role in determining tissue tropism. In an attempt to define the sequences essential for Moloney murine sarcoma virus (Mo-MSV) enhancer activity, we have undertaken deletion and insertion mutational analyses of the 72-bp repeats. We demonstrate that, in contrast to simian virus 40 (SV40), the proximal 19 bp of the 72-bp unit of Mo-MSV are not essential for enhancer function in mouse fibroblasts. Our analysis further localizes the 5' boundary of the Mo-MSV enhancer to a 5'-CAGGAT-3' region.
authors.forename: F
authors.initials: F
authors.lastname: Schulze
authors.forename: E
authors.initials: E
authors.lastname: Boehnlein
authors.forename: P
authors.initials: P
authors.lastname: Gruss
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase; D002874:Chromosome Mapping; D004274:DNA, Recombinant; D004742:Enhancer Elements, Genetic; D005809:Genes, Regulator; D051379:Mice; D008980:Moloney murine sarcoma virus / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004274:DNA, Recombinant; D012333:RNA, Messenger; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase