[Determination of kinase activity by using an enzyme electrode].
A method for determining the enzymatic activity of hexokinases, acetate kinase and pyruvate kinase using an enzyme electrode was developed. The assay time is 2-3 min. The lower limit of the activity determining is 0,054 U/ml. The proposed method was applied to investigation of pyruvate kinase and acetate kinase reactivation under the action of mercaptoethanol.
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D000084:Acetate Kinase / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D002021:Buffers; D004796:Clinical Enzyme Tests / Q000379:methods; D004566:Electrodes; D006593:Hexokinase / Q000032:analysis; D008567:Membranes, Artificial; D008623:Mercaptoethanol / Q000494:pharmacology; D010770:Phosphotransferases / Q000032:analysis*; D011770:Pyruvate Kinase / Q000032:analysis; D012996:Solutions
D002021:Buffers; D008567:Membranes, Artificial; D012996:Solutions; D008623:Mercaptoethanol; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D006593:Hexokinase; D011770:Pyruvate Kinase; D000084:Acetate Kinase
Opredelenie aktivnosti kinaz pri pomoshchi fermentnogo élektroda.