title: Mammalian collagenase predisposes bone surfaces to osteoclastic resorption.
abstract: The cell-free endocranial surface of young adult rat parietal bones was used as a substrate for bone cell-derived mammalian collagenase. Incubation of parietal bones in a concentration of enzyme comparable to that secreted by osteoblastic cells in vitro caused destruction of surface osteoid, and resulted in exposure of mineral onto the bone surface. Bones so pre-treated were considerably more susceptible to osteoclastic resorption than bones pre-incubated in the absence of collagenase. These results are consistent with the view that the osteoid layer which covers bone surfaces acts as a barrier to osteoclastic contact with underlying, resorption-stimulating bone mineral; and that cells of the osteoblastic lineage induce osteoclastic resorption through collagenase secretion which, by digestion of the surface osteoid, exposes bone mineral to osteoclastic contact.
authors.forename: T J
authors.initials: TJ
authors.lastname: Chambers
authors.forename: J A
authors.initials: JA
authors.lastname: Darby
authors.forename: K
authors.initials: K
authors.lastname: Fuller
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects; D003012:Microbial Collagenase / Q000494:pharmacology*; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010294:Parietal Bone; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D003012:Microbial Collagenase