The effect of detergents on immunoprecipitability of lysosomal sphingomyelinase.
Antibodies raised against the soluble form of acid sphingomyelinase from human urine and placenta are able to precipitate about 70% of the sphingomyelinase activity present in preparations of urinary sphingomyelinase. In contrast, no precipitation of sphingomyelinase activity occurs in detergent-containing preparations from placenta or splenic membranes. The formation of immune complexes between the antibodies and urinary sphingomyelinase is inhibited if detergents are added. With the non-ionic detergent Triton X-100 significant inhibition occurs only above the critical micellar concentration of the detergent. With the anionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulphonate (Chaps) substantial inhibition is already observed below the critical micellar concentration of the detergent.
Driessen
M
M
Weitz
G
G
Brouwer-Kelder
E M
EM
Donker-Koopman
W E
WE
Bastiaannet
J
J
Sandhoff
K
K
Barranger
J A
JA
Tager
J M
JM
Schram
A W
AW
D000818:Animals; D002793:Cholic Acids / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D006801:Humans; D007106:Immune Sera; D007163:Immunosorbent Techniques; D008247:Lysosomes / Q000201:enzymology*; D017830:Octoxynol; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D010920:Placenta / Q000201:enzymology / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits; D013053:Spectrophotometry; D013108:Sphingomyelin Phosphodiesterase / Q000032:analysis* / Q000652:urine; D013154:Spleen / Q000201:enzymology; D013501:Surface-Active Agents / Q000494:pharmacology*
D002793:Cholic Acids; D003902:Detergents; D007106:Immune Sera; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate