title: Effect of chronic desmethylimipramine or electroconvulsive shock on selected brain and platelet neurotransmitter recognition sites.
abstract: Rats were treated with electroconvulsive shock (ECS), desmethylimipramine (DMI), ECS plus DMI, or diazepam. In vitro analyses showed that chronic ECS produced an elevated density of recognition sites for [3H]imipramine (IMI) in platelet membranes, but had no effect on membrane preparations derived from cortical tissue. A similar elevation in receptor binding was seen exclusively in platelets after chronic ECS plus DMI, whereas no effect was observed with DMI alone. Equilibrium dissociation constant (KD) values for [3H]IMI were also increased in platelet membranes from rats given chronic ECS or ECS plus DMI treatment. Chronic ECS or DMI administration produced a decreased density of beta-adrenergic recognition sites in frontal cortex and cerebellum as assessed by [3H]dihydroalprenolol (DHA) binding. The combination of ECS plus DMI produced a similar decrease. In addition, chronic diazepam administration produced a down-regulation of the beta-adrenergic receptor only in the cerebellum. These data provide evidence for the differential regulation of brain and peripheral neurotransmitter recognition sites.
authors.forename: M S
authors.initials: MS
authors.lastname: Abel
authors.forename: D E
authors.initials: DE
authors.lastname: Clody
authors.forename: L P
authors.initials: LP
authors.lastname: Wennogle
authors.forename: L R
authors.initials: LR
authors.lastname: Meyerson
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000032:analysis*; D001923:Brain Chemistry / Q000187:drug effects*; D002352:Carrier Proteins; D003891:Desipramine / Q000494:pharmacology*; D004082:Dihydroalprenolol / Q000378:metabolism; D004597:Electroshock; D007099:Imipramine / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000032:analysis*; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter / Q000032:analysis*
chemical_list: D002352:Carrier Proteins; D011943:Receptors, Adrenergic, beta; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter; C027775:imipramine receptor; D004082:Dihydroalprenolol; D007099:Imipramine; D003891:Desipramine