Individual tumors of multifocal EB virus-induced malignant lymphomas in tamarins arise from different B-cell clones.
Cotton-top tamarins were inoculated with sufficient Epstein-Barr virus to induce multiple tumors in each animal within 14 to 21 days. The tumors consisted of large-cell lymphomas that contained multiple copies of the Epstein-Barr virus genome and generated Epstein-Barr virus-carrying cell lines showing no detectable consistent chromosomal abnormality. Hybridization of tumor DNA with immunoglobulin gene probes revealed that each lymphoma was oligo- or monoclonal in origin and that individual tumors from the same animal arose from different B-cell clones. Thus the virus induced multiple transformation events in tamarins in vivo to cause malignant tumors resembling the Epstein-Barr virus-associated lymphomas of patients with organ transplants.
Cleary
M L
ML
Epstein
M A
MA
Finerty
S
S
Dorfman
R F
RF
Bornkamm
G W
GW
Kirkwood
J K
JK
Morgan
A J
AJ
Sklar
J
J
D000818:Animals; D001402:B-Lymphocytes / Q000382:microbiology*; D002051:Burkitt Lymphoma / Q000235:genetics / Q000382:microbiology*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000235:genetics; D016027:Heart Transplantation; D004854:Herpesvirus 4, Human; D006801:Humans; D009374:Neoplasms, Experimental / Q000235:genetics / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012452:Saguinus
D004273:DNA, Neoplasm