title: Regulation by heme of the synthesis of cytochrome C oxidase subunits V and VII in yeast.
abstract: The effects of heme on the synthesis of subunits V and VII of cytochrome c oxidase were examined in the heme-deficient yeast mutant, GL1. In vitro translation and immunodetection with subunit-specific antisera indicated a 50% decrease in both proteins, with RNA obtained from hemeless cells. Unsupplemented, pulse-labeled cells contained both V and VII polypeptides, but at extremely low levels as compared with those found in delta-aminolevulinic acid-supplemented cells. The data suggest that heme controls the formation of mRNAs for the two subunits, and may also have a regulatory role in translation and in the stability of the polypeptides.
authors.forename: E G
authors.initials: EG
authors.lastname: Gollub
authors.forename: J
authors.initials: J
authors.lastname: Dayan
mesh_terms: D003576:Electron Transport Complex IV / Q000096:biosynthesis* / Q000235:genetics; D006418:Heme / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D011487:Protein Conformation; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D006418:Heme; D003576:Electron Transport Complex IV