OK-432 and IL-2-augmented cytotoxicity of human natural killer cells and cytotoxic T lymphocytes at the clonal level.
The biological response modifiers OK-432 and interleukin-2 (IL-2) were found to enhance the lytic capacity of cloned CD3- natural killer (NK) cells and CD3+ T cells. With respect to NK cells, only those clones with a high proliferative capacity and cultured without phytohaemagglutinin (PHA) responded with enhanced lytic capacity to OK-432. OK-432, but not IL-2, was found to augment the antibody-dependent cellular cytotoxicity of cloned NK cells. With T-cell clones, OK-432 augmented the cellular cytotoxicity of CD3+8+ but not that of CD3+4+ cytotoxic T lymphocytes, while IL-2 augmented the cytotoxicity of both types of clone. Taken together, these results indicate that OK-432-augmented lytic capacity is not restricted to NK cells and its pathway of action may be independent of IL-2.
Gravekamp
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Department of Immunology, Dr. Daniel Den Hoed Cancer Center, Rotterdam Radiotherapeutic Institute, The Netherlands.
Vreugdenhil
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Bolhuis
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RL
D000943:Antigens, Differentiation / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001688:Biological Products / Q000494:pharmacology*; D017252:CD3 Complex; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D010844:Picibanil / Q000494:pharmacology*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D011961:Receptors, Fc / Q000502:physiology; D017452:Receptors, IgG; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology
D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D001688:Biological Products; D017252:CD3 Complex; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D011961:Receptors, Fc; D017452:Receptors, IgG; D010844:Picibanil