Occlusion of Rb+ by detergent-solubilized (Na+ + K+)-ATPase from shark salt glands.
Occlusion of Rb+ by C12E8-solubilized (Na+ + K+)-ATPase from shark salt glands has been measured. The rate of de-occlusion at room temperature is about 1 s-1, which is the same as for the membrane-bound enzyme. The amount of Rb+ occluded is 3 moles Rb+ per mole membrane-bound shark enzyme, whereas only about 2 moles Rb+ are occluded by the C12E8-solubilized enzyme.
Esmann
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D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003902:Detergents; D004284:Dogfish; D007700:Kinetics; D011485:Protein Binding; D012413:Rubidium / Q000378:metabolism*; D012491:Salt Gland / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D012995:Solubility; D013329:Structure-Activity Relationship
D003902:Detergents; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium