title: [Characterization and differentiation of rat preadipocytes in primary culture].
abstract: Using a density gradient medium, we isolated homogeneous cell populations from the inguinal tissue of 3-day old rats. In primary culture we obtained, for the first time, the differentiation of 99% of the cells in the presence of a physiological concentration of insulin (10(-9) M). This model closely mimicked the events occurring during normal mammalian adipose development, i.e. a positive change in beta-adrenergic sensitivity, early induction of lipoprotein lipase, expression of the enzymes involved in the triglyceride systems, and the development of responsiveness to glucagon.
authors.forename: A M
authors.initials: AM
authors.lastname: Gaben-Cogneville
authors.forename: A
authors.initials: A
authors.lastname: Quignard-Boulangé
authors.forename: Y
authors.initials: Y
authors.lastname: Aron
authors.forename: L
authors.initials: L
authors.lastname: Brigant
authors.forename: T
authors.initials: T
authors.lastname: Jahchan
authors.forename: J Y
authors.initials: JY
authors.lastname: Pello
authors.forename: B
authors.initials: B
authors.lastname: Poussin
authors.forename: E
authors.initials: E
authors.lastname: Swierczewski
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D005934:Glucagon / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000494:pharmacology; D051381:Rats; D013234:Stem Cells / Q000166:cytology; D014280:Triglycerides / Q000378:metabolism
chemical_list: D007328:Insulin; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D005934:Glucagon; D000242:Cyclic AMP; D008071:Lipoprotein Lipase; D007545:Isoproterenol
vernacular_title: Caractérisation et différenciation de préadipocytes de rats en culture primaire.