title: Plasma levels of alpha-atrial natriuretic peptide in normal subjects under various postural conditions.
abstract: The present paper describes a radioimmunoassay, in conjunction with an extraction procedure on octadecasilyl silica cartridges, of alpha-atrial natriuretic peptide in human plasma and its application in normal subjects under various postural conditions. The sensitivity of this assay is 1.96 pg/ml. The plasma concentrations of alpha-atrial natriuretic peptide in 18 normal male subjects ranged from 10 to 55 pg/ml. This assay was shown to be reproducible as indicated by the low within- and between-assay coefficients of variation. The plasma alpha-ANP concentration decreased during prolonged storage of the plasma at -20 degrees C or -80 degrees C and its level is also lower in hemolyzed plasma samples. The plasma alpha-ANP concentration is similar in standing and supine position but rose significantly when the subjects were tilted 20 degrees head-down.
authors.affiliation: Department of Pathophysiology, University of Leuven, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Lijnen
authors.forename: J R
authors.initials: JR
authors.lastname: M'Buyamba-Kabangu
authors.forename: J
authors.initials: J
authors.lastname: Huysecom
authors.forename: R
authors.initials: R
authors.lastname: Fagard
authors.forename: J
authors.initials: J
authors.lastname: Staessen
authors.forename: A
authors.initials: A
authors.lastname: Amery
mesh_terms: D000450:Aldosterone / Q000097:blood; D009320:Atrial Natriuretic Factor / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D011187:Posture; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012083:Renin / Q000097:blood; D013696:Temperature
chemical_list: D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D012083:Renin