title: Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression.
abstract: Derivatives of plasmid pRK290 that are useful for cloning and for analyzing gene expression in a wide variety of Gram-negative bacteria are described. A smaller broad host range plasmid derived from RK2, with properties similar to that of pRK290, is also described.
authors.forename: G
authors.initials: G
authors.lastname: Ditta
authors.forename: T
authors.initials: T
authors.lastname: Schmidhauser
authors.forename: E
authors.initials: E
authors.lastname: Yakobson
authors.forename: P
authors.initials: P
authors.lastname: Lu
authors.forename: X W
authors.initials: XW
authors.lastname: Liang
authors.forename: D R
authors.initials: DR
authors.lastname: Finlay
authors.forename: D
authors.initials: D
authors.lastname: Guiney
authors.forename: D R
authors.initials: DR
authors.lastname: Helinski
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007763:Lac Operon; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase