Steroid conversions by the 19-day old foetal rat ovary in organ culture.
The ovaries from 19-day old foetal rats were cultured in vitro on Wolff and Haffen's semi-solid medium with radioactive progesterone, dehydroepiandrosterone, testosterone or androstenedione added as precursors, and in the presence or absence of lutropin, follitropin and human chorion gonadotropin. After a 24-h culture period, the media were analysed for the presence of testosterone, androstenedione, oestrone and oestradiol, which were measured by specific activity determination after isotopic dilution.
Weniger
J P
JP
Chouraqui
J
J
Zeis
A
A
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D004970:Estrone / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D009924:Organ Culture Techniques; D010053:Ovary / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000378:metabolism
D006063:Chorionic Gonadotropin; D012739:Gonadal Steroid Hormones; D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015067:17-Hydroxysteroid Dehydrogenases; C032690:3-alpha-(17-beta)-hydroxysteroid dehydrogenase (NAD(+))