Anti-idiotypes against a monoclonal anti-haloperidol antibody bind to dopamine receptor.
Anti-idiotypic antibodies were raised in rabbits by immunization with a monoclonal anti-haloperidol antibody. Some of these anti-idiotypic antibodies bind in a concentration dependent manner to bovine striatal membranes. Following affinity purification, these antibodies inhibit haloperidol binding to striatal membranes and deplete [3H]-spiperone binding sites from a solubilized preparation of striatal membranes. It is thus concluded that these anti-idiotypic antibodies are an internal image of haloperidol and as such can interact with D2-dopamine receptors.
Elazar
Z
Z
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Kanety
H
H
Schreiber
M
M
Fuchs
S
S
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D002417:Cattle; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D003342:Corpus Striatum / Q000276:immunology / Q000378:metabolism; D006220:Haloperidol / Q000276:immunology* / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007163:Immunosorbent Techniques; D051379:Mice; D011817:Rabbits; D011954:Receptors, Dopamine / Q000276:immunology* / Q000378:metabolism; D017448:Receptors, Dopamine D2
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000941:Antigens; D007130:Immunoglobulin Idiotypes; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D006220:Haloperidol