Hydrolysis of histones by proteinases.
Hydrolysis of histones by proteinases from rat liver, skin and other sources was studied by using a rat thymus histone preparation as the substrate and polyacrylamide-gel electrophoresis and densitometric analysis as the methods to detect histone subtypes and their hydrolysis. The rat mast-cell proteinase I effectively hydrolysed histones except type H4. Thrombin hydrolysed effectively histones H1 and H2A, whereas plasmin hydrolysed all types of histones. Cathepsin D hydrolysed especially histone H2A. Cathepsins B and L hydrolysed all histones more slowly, and cathepsin H hydrolysed them extremely slowly. Epidermal aminoendopeptidase did not hydrolyse histones. Trypsin and chymotrypsin were used as reference enzymes, which hydrolysed all types of histones in very low concentrations. This study suggests that a variety of proteinases could play a role in histone hydrolysis. Hydrolysis of a specific subtype of histones, such as histone H2A at pH 6 by cathepsin D, may be directly involved in regulation of epidermal-cell differentiation.
Harvima
R J
RJ
Department of Dermatology, University of Kuopio, Finland.
Yabe
K
K
Fräki
J E
JE
Fukuyama
K
K
Epstein
W L
WL
D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D002403:Cathepsins / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D004817:Epidermis / Q000201:enzymology; D005341:Fibrinolysin / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013917:Thrombin / Q000378:metabolism
D006657:Histones; D002403:Cathepsins; D010450:Endopeptidases; D000626:Aminopeptidases; D013917:Thrombin; D005341:Fibrinolysin