Trans-activation of the human immunodeficiency virus long terminal repeat sequences, expressed in an adenovirus vector, by the adenovirus E1A 13S protein.
The human immunodeficiency virus 1 (HIV-1) long terminal repeat (LTR) sequences were inserted into adenovirus in place of the E1 region. The HIV-1 LTR contained in this recombinant adenovirus responds to trans-activation by tatIII in a HeLa cell line constitutively expressing that HIV-1 gene product. In addition, the HIV-1 LTR is activated by the adenovirus E1A 13S, but not 12S or 9S, gene product when it is supplied in trans by a coinfecting wild-type adenovirus. The Rous sarcoma virus LTR, in a similar recombinant adenovirus, is insensitive to tatIII but is also trans-activated by the E1A 13S protein. The action of the 13S E1A and tatIII proteins are additive for the HIV-1 LTR in the context of adenovirus and they appear to act at the transcriptional level. As in HeLa cells, the adenovirus-borne HIV-1 LTR is inactive in the absence of a trans-activator in H9 and Jurkat cells, two human leukemic T-cell lines. This suggests that recombinant adenoviruses have diagnostic potential for the detection of trans-activators of the HIV-1 LTR that are present in circulating human lymphocytes.
Rice
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AP
Cold Spring Harbor Laboratory, NY 11724.
Mathews
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MB
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics*; D000957:Antigens, Viral, Tumor; D002460:Cell Line; D005786:Gene Expression Regulation; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D006678:HIV / Q000235:genetics*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
D017456:Adenovirus Early Proteins; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral; D014764:Viral Proteins