Production of D- and L-xylulose by mutants of Klebsiella pneumoniae and Erwinia uredovora.
D-Xylulose and L-xylulose were produced biologically by the oxidation of a corresponding pentitol. A Klebsiella pneumoniae mutant was constructed for the oxidation of D-arabitol to D-xylulose. This mutant constitutively synthesized the D-arabitol permease system and D-arabitol dehydrogenase but was unable to produce the D-xylulokinase of the D-arabitol pathway or the D-xylose isomerase and D-xylulokinase of the D-xylose pathway. An Erwinia uredovora mutant which constitutively synthesized a novel xylitol-4-dehydrogenase but could not synthesize L-xylulokinase was used for the oxidation of xylitol to L-xylulose. Washed cell suspensions of either mutant incubated with 0.5% pentitol would oxidize 60 to 65% of the pentitol to the corresponding ketopentose in 18 h and excrete the ketopentose into the medium. Ketopentoses were rapidly purified from the remaining pentitol by hydroxyl affinity chromatography.
Doten
R C
RC
Mortlock
R P
RP
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000378:metabolism; D004885:Erwinia / Q000235:genetics* / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010429:Pentoses / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D013237:Stereoisomerism; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D013402:Sugar Alcohols / Q000378:metabolism; D014993:Xylitol / Q000378:metabolism; D014996:Xylulose / Q000096:biosynthesis*
D010429:Pentoses; D013402:Sugar Alcohols; D014996:Xylulose; D013401:Sugar Alcohol Dehydrogenases; C022751:D-arabinitol dehydrogenase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D014993:Xylitol; C014999:arabitol