SnRNP core protein enrichment in the nuclear matrix.
The D protein (16 kDa) is part of a protein core, common to U1, U2, U5, U4/U6 small nuclear RNA containing ribonucleoprotein particles. Monoclonal antibodies reactive with the D protein were used in quantitative dot blotting and Western blotting to demonstrate that this protein was a component of salt resistant nuclear structures and was enriched greater than 3 to 5-fold in RNAase-protected nuclear matrix preparations.
Harris
S G
SG
Department of Pathology and Laboratory Medicine, University of Rochester, NY 14642.
Smith
H C
HC
D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000032:analysis; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D012260:Ribonucleases / Q000378:metabolism; D012261:Ribonucleoproteins / Q000032:analysis*; D017411:Ribonucleoproteins, Small Nuclear
D000911:Antibodies, Monoclonal; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D012260:Ribonucleases