Modulation of the activity of the platelet-derived growth factor receptor by phorbol myristate acetate.
Phorbol myristate acetate (PMA) weakly activates Na+/H+ exchange in NR-6 cells. Simultaneously, PMA blocks the activation of Na+/H+ exchange by platelet-derived growth factor or by serum. Phorbol esters that do not activate protein kinase C do not show this metabolic response. We conclude that activation of Na+/H+ exchange by platelet-derived growth factor or serum does not require the intermediate activation of protein kinase C. We postulate from this and previous observations that a major role of protein kinase C is to act as an inhibitor of the activity of cell surface receptors, in particular mitogen receptors.
Whiteley
B
B
Deuel
T
T
Glaser
L
L
D000818:Animals; D001769:Blood; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D005260:Female; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000032:analysis; D010704:Phorbols / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor; D017923:Sodium-Hydrogen Exchangers; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002352:Carrier Proteins; D010704:Phorbols; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D017923:Sodium-Hydrogen Exchangers; D017479:Receptors, Platelet-Derived Growth Factor; D013755:Tetradecanoylphorbol Acetate