Phase-contrast microscopy of the primate retina.
The study of hematoxylin and eosin stained thick sections (15 microns) of the primate retina with the phase-contrast microscope provided a means for the selective demonstration of many cellular structures that could not be resolved with the same degree of detail which was possible when bright-field microscopy was used, or when phase-contrast microscopy was employed to examine unstained material. The H & E-stain greatly enhanced the phase-contrast image, so that cytoplasmic structure, fiber trajectories, and gross synaptic detail of the retina could be demonstrated to better advantage.
Fix
J D
JD
Department of Anatomy, Marshall University School of Medicine, Huntington, West Virginia 25701.
D000818:Animals; D008253:Macaca mulatta; D008858:Microscopy, Phase-Contrast / Q000379:methods; D012160:Retina / Q000166:cytology*