title: Constitutively phosphorylated residues in the NS protein of vesicular stomatitis virus.
abstract: The NS protein of vesicular stomatitis virus is an auxiliary protein in the virus core (nucleocapsid) that plays a role in virus-specific RNA synthesis. NS exhibits a variety of phosphorylated forms, and the degree of phosphorylation correlates with the rate of RNA synthesis. However, chymotryptic peptide mapping has indicated that all forms of NS share a common cluster of phosphorylated residues. To locate these residues in the primary structure of the molecule, we performed a series of residue-specific chemical and enzymatic cleavages and separated radiophosphate-labeled peptides by gel electrophoresis. The data indicate that the constitutively phosphorylated sites in NS molecules reside in the amino-terminal region of the molecule, between residues 35 and 78. The previously reported resistance of the phosphoamino acids in this region to dephosphorylation by exogenous phosphatase suggests that this domain is embedded within the tertiary structure of the molecule or involved in quaternary interactions. In contrast, the amino acid residues that are phosphorylated secondarily, making NS more active in RNA synthesis, reside in more exposed regions of the molecule.
authors.forename: C H
authors.initials: CH
authors.lastname: Hsu
authors.forename: D W
authors.initials: DW
authors.lastname: Kingsbury
mesh_terms: D001224:Aspartic Acid; D002918:Chymotrypsin / Q000494:pharmacology; D003488:Cyanogen Bromide; D008715:Methionine; D008970:Molecular Weight; D010748:Phosphopeptides / Q000032:analysis*; D010766:Phosphorylation; D011392:Proline; D014357:Trypsin / Q000494:pharmacology; D014364:Tryptophan; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis*; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D010748:Phosphopeptides; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D001224:Aspartic Acid; D014364:Tryptophan; D011392:Proline; D008715:Methionine; D002918:Chymotrypsin; D014357:Trypsin; D003488:Cyanogen Bromide