Helper T lymphocytes which recognize the MHC class I alloantigens in vivo are CD4+CD8-1.
To determine the CD4 or CD8 phenotype of the Th lymphocyte which recognizes in vivo the MHC class I alloantigens, B10 recombinant mice were treated with anti-CD8 or anti-CD4 mAb and immunized with lymphoid cells from donors differing in the K or D region of the MHC. Alloantibodies were evaluated by a 51Cr-release assay or by indirect immunofluorescence. The production of IgG anti-Dd and anti-Kk alloantibodies was increased by the deletion of the CD8+ and absent in mice depleted of the CD4+ subset. These experiments indicate that the helper influence elicited by the recognition of a MHC class I alloantigen in vivo is due to cells of the CD4+CD8- phenotype.
Piguet
P F
PF
Department of Pathology, University of Geneva, Switzerland.
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D000961:Antilymphocyte Serum / Q000032:analysis; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000008:administration & dosage / Q000276:immunology*; D007518:Isoantibodies / Q000032:analysis; D008212:Lymphocyte Depletion; D051379:Mice; D010641:Phenotype; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification* / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D000961:Antilymphocyte Serum; D015395:Histocompatibility Antigens Class I; D007518:Isoantibodies