Production of cell-mediated immune response to herpes simplex virus by immunization with anti-idiotypic heteroantisera.
Three BALB/c monoclonal antibodies capable of neutralizing herpes simplex virus type 1 (HSV-1) were used to prepare rabbit anti-idiotypic antisera. Affinity-purified antibodies from four of these rabbits were used to immunize mice by repeated subcutaneous injection over a period of 6 to 7 weeks: the mice were then challenged with HSV-1 subcutaneously in the ear pinna. Measurement of ear swelling showed that prior administration of anti-idiotypic serum could generate dose-dependent delayed-type hypersensitivity responses.
Gell
P G
PG
Moss
P A
PA
D000818:Animals; D000910:Antibodies, Heterophile / Q000008:administration & dosage*; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007111:Immunity, Cellular; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007249:Inflammation; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000276:immunology*
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes