Effect of gangliosides on primary and secondary plaque-forming cells responses and on suppressor T-cells induction.
This study was designed to determine the suppressive effect of bovine brain gangliosides on primary and secondary anti-sheep red blood cell (SRBC) plaque-forming cells (PFC) response. Spleen cells from inbred C57BL/6J mice were used as sources of lymphocytes. The result showed that levels of gangliosides at 50 and 100 micrograms/ml suppressed primary anti-SRBC PFC responses about 30% and 60%, respectively, of normal control. In contrast, secondary anti-SRBC PFC were not suppressed by the same levels of gangliosides. Likewise, PFC response from mixing SRBC-primed T-cells with naive B-cells was not suppressed by gangliosides. In addition, induction of suppressor T-cells was reduced by 50 and 100 micrograms/ml of gangliosides, 57% and 63%, respectively, versus 86% of suppression of PFC response by the control suppressor T-cells. These results further suggest the role of gangliosides as immunomodulators.
Jeng
K C
KC
Department of Internal Medicine, Taichung Veterans General Hospital, Taiwan, ROC.
Lan
J L
JL
Chou
H F
HF
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D003864:Depression, Chemical; D004912:Erythrocytes / Q000276:immunology; D005732:Gangliosides / Q000494:pharmacology*; D006462:Hemolytic Plaque Technique; D007114:Immunization; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D012756:Sheep; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
D005732:Gangliosides