title: Complex rearrangements within the human J delta-C delta/J alpha-C alpha locus and aberrant recombination between J alpha segments.
abstract: We have examined DNA rearrangements within a 120 kb cloned region of the human T cell receptor J delta-C delta/J alpha-C alpha locus. Three types of pattern emerge from an analysis of T cell lines and clones. Firstly, cells with two rearrangements within J delta-C delta; secondly, cells with one rearrangement within J delta-C delta and one or more J alpha rearrangements, and finally, cells with rearrangements within J alpha and consequential deletion of the delta locus. Further analysis by cloning of rearrangements within the J alpha locus show that, in addition to V alpha-J alpha joins, J alpha-J alpha aberrant recombinations occur and rearrangement data indicate that such events are frequent. A model is presented to account for such recombinations.
authors.affiliation: Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: R
authors.initials: R
authors.lastname: Baer
authors.forename: T
authors.initials: T
authors.lastname: Boehm
authors.forename: H
authors.initials: H
authors.lastname: Yssel
authors.forename: H
authors.initials: H
authors.lastname: Spits
authors.forename: T H
authors.initials: TH
authors.lastname: Rabbitts
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D005796:Genes; D006801:Humans; D007938:Leukemia / Q000473:pathology; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta