Cloning of the Escherichia coli recJ chromosomal region and identification of its encoded proteins.
A 9.6-kilobase BamHI-SalI fragment carrying recJ+ was cloned into vector pBR322. Deletion and transposon mutagenesis were used to map the recJ gene on this fragment. The maxicell protein-labeling technique was used to correlate a functional recJ gene with the presence of a polypeptide of 53,000 apparent molecular weight. Two additional genes, one encoding two proteins of 26,000 and 25,000 Mr and the other encoding a 31,000-Mr protein, were mapped on a 3.7-kilobase HindIII-SalI subfragment with recJ. Functions for these adjacent genes are not known; however, insertion mutations in these genes lessen the expression of the putative recJ protein detected in maxicells. A 9.6-kilobase BamHI-SalI fragment carrying the temperature-sensitive mutation recJ147 was also cloned and used for complementation studies to identify other recJ mutations.
Lovett
S T
ST
Clark
A J
AJ
D001426:Bacterial Proteins / Q000032:analysis*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005816:Genetic Complementation Test; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
D001426:Bacterial Proteins