title: The Neurospora mitochondrial genome: the region coding for the polycistronic cytochrome oxidase subunit I transcript is preceded by a transfer RNA gene.
abstract: We have sequenced a 682 bp fragment of Neurospora crassa mitochondrial DNA to complete the sequence between the gene for cytochrome b and the unassigned reading frame, URF U. The sequence contains a gene for a cysteine tRNA. The 5' end of the 6 kb polycistronic transcript of cytochrome c oxidase subunit 1 is immediately downstream from this tRNA. This shows that also in fungal mitochondria tRNAs can be used as processing signals, whereas palindromic sequences containing double Pst I sites, also present in this region, are not used for processing.
authors.forename: H
authors.initials: H
authors.lastname: de Vries
authors.forename: P
authors.initials: P
authors.lastname: Haima
authors.forename: M
authors.initials: M
authors.lastname: Brinker
authors.forename: J C
authors.initials: JC
authors.lastname: de Jonge
mesh_terms: D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV / Q000235:genetics*; D005796:Genes; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics*; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D003573:Cytochrome b Group; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D003576:Electron Transport Complex IV