Mechanism of skin allograft enhancement across an H-2 class I mutant difference. Evidence for involvement of veto cells.
Intravenous injection of spleen cells across mutant class I H-2 incompatibility results in a drastic donor-specific prolongation of skin allograft survival and a marked decrease in the donor-specific cytotoxic T lymphocyte precursor (CTLp) frequency. This immunosuppressive effect depends on the presence of radiosensitive T cells in the donor cell inoculum. It was excluded that a graft-vs.-host reaction was responsible for the observed effects. In mixing experiments, spleen cells from animals transfused with allogeneic lymphocytes could not suppress a normal CTL response against the alloantigen, despite an excess of putative recipient-derived spleen suppressor cells. The data are compatible with the idea that donor T cells function as veto cells which inactivate recipient CTLp directed against the alloantigen expressed by the veto cell.
Kast
W M
WM
Central Laboratory of the Netherlands, Red Cross Blood Transfusion Service.
Van Twuyver
E
E
Mooijaart
R J
RJ
Verveld
M
M
Kamphuis
A G
AG
Melief
C J
CJ
De Waal
L P
LP
D000818:Animals; D001803:Blood Transfusion; D002470:Cell Survival / Q000528:radiation effects; D006183:H-2 Antigens / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy / Q000379:methods*; D051379:Mice; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D006183:H-2 Antigens