The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor.
Glucocorticoid receptor (GCR) protein stimulates transcription from a variety of cellular genes. We show here that GCR partially purified from rat liver binds specifically to a site within the first intron of the human growth hormone (hGH) gene, approximately 100 base pairs downstream from the start of hGH transcription. GCR binding is selectively inhibited by methylation of two short, symmetrically arranged clusters of guanine residues within this site. A cloned synthetic 24-base-pair deoxyoligonucleotide containing the predicted GCR binding sequence interacts specifically with GCR. The hGH binding site shares sequence homology with a GCR binding site upstream from the human metallothionein II gene and a subset of GCR binding sites from mouse mammary tumor virus. All of these binding sites for this eukaryotic transcriptional regulatory protein show remarkable similarity in overall geometry to the binding sites for several prokaryotic transcriptional regulatory proteins.
Moore
D D
DD
Marks
A R
AR
Buckley
D I
DI
Kapler
G
G
Payvar
F
F
Goodman
H M
HM
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005374:Filtration; D013006:Growth Hormone / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000032:analysis; D009841:Oligonucleotides / Q000378:metabolism; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D014158:Transcription, Genetic
D009841:Oligonucleotides; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D013006:Growth Hormone; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes