Morphological investigations in human islets of Langerhans isolated by the Velcro-technic.
The lower yield of viable, isolated islets from one donor pancreas and the immunogenicity are responsible for disappointing results in human islet transplantation. Our preliminary results in the application of Velcro-technic (Lacy, 1982) studied by morphological investigations demonstrate the feasibility of this technic in human islet isolation. If we compare the influence of Velcro-technic with regard to the first step of Velcro-technic, the duct perfusion, we can conclude that A-cell granules, stained by Grimelius, are reduced during the collagenase perfusion. On the other side, we recognized B-cell granules up to 60 min after duct perfusion. Connective tissue is reduced by the isolation technic and absent completely up to the Ficoll separation. The exocrine tissue of the pancreas is damaged continuously, and after Ficoll separation we detected singular acinar cells only. The yield from the used lienal human pancreas were about 80 000 islets. Islets after Ficoll separation, studied by electron microscopic investigation, showed non-damaged A and B cells with granules in the typical manner.
Kühn
F
F
Schulz
H J
HJ
Lorenz
D
D
Hahn
H J
HJ
Lippert
H
H
Abri
O
O
Wilke
T
T
D002469:Cell Separation / Q000379:methods*; D006801:Humans; D016381:Islets of Langerhans Transplantation; D003012:Microbial Collagenase
D003012:Microbial Collagenase