Demonstration of receptors for a PDGF-like mitogen on human osteosarcoma cells.
U-2 OS human osteosarcoma cells synthesize, process and secrete a platelet-derived growth factor (PDGF)-like mitogen. Incubation of these cells with 1 mM suramin unmasks PDGF receptor sites which are normally occupied or down regulated by the secreted endogenous PDGF-like mitogen. Partially purified preparations of metabolically labelled U-2 OS conditioned medium binds to U-2 OS cells and binding is inhibited by excess PDGF. These findings suggest that U-2 OS cells are capable of autocrine stimulation.
Graves
D T
DT
Owen
A J
AJ
Antoniades
H N
HN
D001667:Binding, Competitive; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D012516:Osteosarcoma / Q000032:analysis*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis*; D017479:Receptors, Platelet-Derived Growth Factor; D013498:Suramin / Q000494:pharmacology
D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D013498:Suramin; D004815:Epidermal Growth Factor; D017479:Receptors, Platelet-Derived Growth Factor