Purification of non-synaptic and synaptic mitochondria and plasma membranes from rat brain by a rapid Percoll gradient procedure.
A method is described for the isolation and purification of non-synaptic and synaptic mitochondria and plasma membranes. The procedure involved differential centrifugation and successively a Ficoll gradient followed by a Percoll one. The method is rapid and maintains constant osmotic conditions. The Percoll-purified fractions show a lower density than those prepared by the conventional methods. The fractions were identified and characterized by enzyme markers and electron microscopy. The method will be useful for routine preparations rendering a good degree of homogeneity and purity.
Rendon
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Masmoudi
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D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000201:enzymology; D002499:Centrifugation, Density Gradient / Q000379:methods; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis; D011205:Povidone; D051381:Rats; D011919:Rats, Inbred Strains; D012822:Silicon Dioxide; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis; D013347:Subcellular Fractions / Q000648:ultrastructure; D013574:Synaptosomes / Q000201:enzymology* / Q000378:metabolism
C016039:Percoll; D012822:Silicon Dioxide; D009251:NADPH-Ferrihemoprotein Reductase; D000135:Acid Phosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D011205:Povidone