Antimycin binds to a small subunit of the ubiquinol: cytochrome c oxidoreductase.
Bovine heart ubiquinol: cytochrome c oxidoreductase in Triton X-100 is split with guanidine into a number of fractions. A new method for measuring antimycin binding is developed using extraction with pentanol of the reversibly bound antimycin. By this method and the normal titration method, antimycin-binding capacity is found in a fraction containing a small subunit with a molecular mass of about 12000. This polypeptide was associated with cytochrome c1 but is probably not the 'hinge protein'. Fractions that contain cytochrome b did not show binding by the pentanol-extraction method.
van Keulen
M A
MA
Berden
J A
JA
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives* / Q000378:metabolism; D001665:Binding Sites; D002417:Cattle; D003573:Cytochrome b Group / Q000378:metabolism; D003575:Cytochromes c1 / Q000378:metabolism; D014450:Electron Transport Complex III; D066298:In Vitro Techniques; D009097:Multienzyme Complexes / Q000032:analysis / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D000439:Pentanols; D011808:Quinone Reductases / Q000032:analysis / Q000378:metabolism*
D000596:Amino Acids; D003573:Cytochrome b Group; D009097:Multienzyme Complexes; D000439:Pentanols; C032456:antimycin; D000968:Antimycin A; D003575:Cytochromes c1; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases; D014450:Electron Transport Complex III; C024999:n-pentanol