Use of a specific monoclonal antibody for studying the liver metastatic invasion of a rat colon cancer.
A simple model for liver metastasis from colon cancer resulted from the intraportal injection of 2 x 10(7) highly tumorigenic DHD/K12/PROb cells into syngeneic BDIX rats. Early detection and development of cancer invasion were studied by conventional microscopy and immunoenzymatic staining using a specific monoclonal antibody. Metastases developed either from isolated cancer cells early disseminated in sinusoid network or from intraportal microthrombi. An intense immune reaction developed until day 15 after injection but decreased and disappeared at the latest stages of evolution.
Chauffert
B
B
Research Group on Human and Experimental Digestive Cancers, INSERM U. 252 Faculty of Medicine, Dijon, France.
Shimizu
T
T
Caignard
A
A
Hammann
A
A
Genne
P
P
Pelletier
H
H
Martin
M S
MS
D000818:Animals; D000911:Antibodies, Monoclonal; D003110:Colonic Neoplasms / Q000473:pathology*; D005260:Female; D007124:Immunoenzyme Techniques; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary*; D008297:Male; D009361:Neoplasm Invasiveness; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal