Nucleotide sequence of a cDNA clone encoding mouse protamine 1.
The nucleotide sequence of a 404-base cDNA encoding the cysteine-rich, tyrosine-containing mouse protamine has been determined. This insert, isolated from a mouse testis cDNA library, encodes a polypeptide of 50 amino acids of which 28 are arginine, 9 are cysteine, and 3 are tyrosine. The insert contains the complete 3' noncoding region of 151 bases and most of the 5' noncoding region. The predicted amino acid sequence of mouse protamine 1 is about 80% homologous to boar protamine and 67% homologous to bull protamine and contains the central, highly basic domain of four arginine clusters found in the trout protamines. The identification of a cDNA clone for a mouse protamine will facilitate studies of the evolution, regulation, and protein-DNA interaction of this nuclear protein unique to haploid spermatogenic cells.
Kleene
K C
KC
Distel
R J
RJ
Hecht
N B
NB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D011479:Protamines / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013737:Testis / Q000378:metabolism*
D003062:Codon; D011479:Protamines; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes