Purification and identification of endogenous anti-opioid substances from bovine brain.
Two fractions with high potency for reversing the inhibitory effect of Met-enkephalin on the electrically induced contractility of guinea-pig ileum have been purified from bovine brain extract. Unexpectedly, one isolated peptide was identified as [Val5]-angiotensin I and the other fraction was [Val5]-angiotensin II, as judged by chromatographic comparisons on HPLC and amino acid analysis. Since angiotensins did not affect opioid binding to brain membrane, we consider that angiotensins may act as physiological antagonists to the opioid system in the brain, as well as in the guinea-pig ileum.
Kaneko
S
S
Tamura
S
S
Takagi
H
H
D000596:Amino Acids / Q000032:analysis; D000803:Angiotensin I / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000804:Angiotensin II / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000809:Angiotensins; D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004744:Enkephalin, Methionine / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D008297:Male; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000378:metabolism
D000596:Amino Acids; D000809:Angiotensins; D011957:Receptors, Opioid; D000804:Angiotensin II; C044451:angiotensin I, Val(5)-; D004744:Enkephalin, Methionine; C020539:angiotensin II, Val(5)-Trp(8)-; D000803:Angiotensin I