Thiophosphorylation and phosphorylation of chromatin proteins from calf thymus in vitro.
Thiophosphorylation and phosphorylation of 5% perchloric acid extractable proteins from calf thymus chromatin were studied using a cyclic GMP-dependent protein kinase from bovine lung and a nuclear protein kinase II from rat liver. The phosphorylation reaction catalyzed by nuclear protein kinase II utilized [gamma -35S]ATP as a phosphate donor almost as efficiently as [gamma -32P]ATP, but the cGMP-dependent protein kinase mediated phosphorylation by [35S]ATP was about 20 times less effective than that by [32P]ATP. In addition, using [35S]ATP instead of [32P]ATP changed markedly the cGMP-dependent phosphorylation pattern of the PCA-extractable proteins as examined by gel electrophoresis. Thus, depending on the type of protein kinase, the results from thiophosphorylation and phosphorylation reactions may vary considerably.
Palvimo
J
J
Linnala-Kankkunen
A
A
Mäenpää
P H
PH
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D006152:Cyclic GMP / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006609:High Mobility Group Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013950:Thymus Gland / Q000378:metabolism*
D000596:Amino Acids; D006609:High Mobility Group Proteins; D010710:Phosphates; D011494:Protein Kinases; C026813:protein kinase NII; D006152:Cyclic GMP; C035638:thiophosphoric acid