title: Fucokinase, its anomeric specificity and mechanism of phosphate group transfer.
abstract: Fucokinase phosphorylates L-fucose at the anomeric position and, as such, might use either the alpha or beta anomer as its substrate. Examination of the utilization of radiolabelled alpha and alpha,beta mixtures established beta-L-fucose as the required substrate. Phosphorylation at the anomeric center might involve either the loss or retention of the anomeric oxygen. The mechanism has been shown to involve anomeric oxygen retention through mass spectrometric analysis of the product phosphate derived from 18O-labelled L-fucose.
authors.forename: W
authors.initials: W
authors.lastname: Butler
authors.forename: G S
authors.initials: GS
authors.lastname: Serif
mesh_terms: D005643:Fucose; D066298:In Vitro Techniques; D010100:Oxygen; D010710:Phosphates; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D010710:Phosphates; D005643:Fucose; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020153:fucokinase; D010100:Oxygen