Activation and expansion of hapten- and protein-specific T helper cells from nonsensitized mice.
Hapten- and protein-antigen-specific T helper cells are usually expanded in vitro from lymphocytes obtained from sensitized animals. In this paper we report on the primary activation and proliferation in vitro of T helper cells from nonsensitized animals by using syngeneic cultured epidermal Langerhans cells as a source of potent antigen-presenting cells. The primary in vitro proliferation was blocked with monoclonal antibodies to Ia molecules, to lymphocyte function-associated antigen 1 (LFA-1), and to L3T4. T helper cell populations sensitized in vitro to haptens and protein antigens showed hapten- and antigen-specific proliferation when restimulated in vitro with spleen cells. Besides its experimental usefulness, in vitro generation of syngeneic specific T helper cells may afford possibilities for adoptive immunotherapy.
Hauser
C
C
Dermatology Branch, National Cancer Institute, Bethesda, MD 20892.
Katz
S I
SI
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D006241:Haptens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007801:Langerhans Cells / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D006241:Haptens; D000949:Histocompatibility Antigens Class II