[Study of 2'-phosphodiesterase activity in cultured NIH 3T3 cells during activation of cAMP-dependent phosphorylation].
A rapid and transient decrease in 2'-phosphodiesterase activity in NIH 3T3 mouse cells was observed after adrenaline addition. The decrease of activity was accompanied by an elevation of intracellular cAMP level. The 2'-phosphodiesterase activity changed similarly when cells sink deeper into the resting state. In the latter case, the fall of the enzyme activity was correlated with elevation of the activity of cAMP-dependent proteinkinase and, moreover, a considerable increase of the intracellular level of 2',5'-oligoadenylate was observed. Phosphorylation of proteins by cAMP-dependent proteinkinase in the cell lysate also produced a pronounced drop of 2'-phosphodiesterase activity. Exogenous 2',5'-oligo (A) treatment of the cells resulted in the rise of 2'-phosphodiesterase activity; actinomycin D prevented this effect. The data presented suggest the involvement of two different mechanisms in regulation of 2'-phosphodiesterase activity: cAMP-dependent phosphorylation and induction of 2'-phosphodiesterase by 2',5'-oligoadenylate.
Kartasheva
O N
ON
Itkes
A V
AV
Turpaev
K T
KT
Tunitskaia
V L
VL
Severin
E S
ES
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D005095:Exoribonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051379:Mice; D009843:Oligoribonucleotides / Q000494:pharmacology; D010766:Phosphorylation; D011433:Propranolol / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*; D013552:Swine
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D003609:Dactinomycin; C023505:2',5'-oligoadenylate; D011433:Propranolol; D000242:Cyclic AMP; D011494:Protein Kinases; D015088:2',5'-Oligoadenylate Synthetase; D005095:Exoribonucleases; C029364:2'-phosphodiesterase; D004837:Epinephrine
Issledovanie aktivnosti 2'-fosfodiésterazy v kul'ture kletok NIH 3T3 pri aktivatsii cAMP-zavisimogo fosforilirovaniia.