title: Characterization of restriction endonuclease maps of hepatitis B viral DNAs.
abstract: The HBV DNA isolated from Dane particles of 9 patients' plasma was cloned into the EcoRI or BamHI site of the pUC8 plasmids. Two plasmids with full length HBV DNA and four plasmids containing the HBV surface antigen gene were obtained. Based on our cloned HBV DNA and a comparison with 7 complete sequences and 5 restriction endonuclease patterns of HBV DNA published by others, we can recognize common restriction sites shared by different subtypes (adw, adr, ayw, and adyw): (1) a HincII site in the S gene, (2) a BamHI site in the X region, and (3) two BglII sites in the C gene. In addition adw has specific sites for HincII, BamHI, and PstI in the pre-S region. A unique XhoI site is present in the pre-S region in all subtypes except for adw.
authors.forename: S J
authors.initials: SJ
authors.lastname: Lo
authors.forename: Y H
authors.initials: YH
authors.lastname: Lee
authors.forename: J S
authors.initials: JS
authors.lastname: Chiou
authors.forename: L P
authors.initials: LP
authors.lastname: Ting
authors.forename: W T
authors.initials: WT
authors.lastname: Liu
authors.forename: K B
authors.initials: KB
authors.lastname: Choo
mesh_terms: D001426:Bacterial Proteins; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; C024404:BglII endonuclease; C034510:CTCGAG-specific type II deoxyribonucleases; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases