An enzyme immunoassay for phospholamban.
Phospholamban, a phosphorylatable protein of the cardiac sarcoplasmic reticulum, has been estimated by a semi-quantitative immunoassay. It can be determined in purified membrane preparations as well as in crude fractions of cardiac muscle membranes, regardless of the phosphorylation state of the phosphoprotein. The content of phospholamban in mammalian heart muscle membrane vesicles correlates with the activity of the calcium/magnesium-dependent ATPase with the exception of the oxalate-loaded membrane preparations. This observation indicates that phospholamban and the calcium transporting enzyme are localized at different sites in cardiac sarcoplasmic reticulum.
Holtzhauer
M
M
Central Institute for Cardiovascular Research, Academy of Sciences, GDR, Berlin.
D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D002135:Calcium-Binding Proteins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology*; D012508:Sarcolemma / Q000201:enzymology; D012519:Sarcoplasmic Reticulum / Q000201:enzymology; D013045:Species Specificity; D013552:Swine; D000099211:Phospholamban
D000251:Adenosine Triphosphatases; D000906:Antibodies; D002135:Calcium-Binding Proteins; D000099211:Phospholamban