Ferricrocin functions as the main intracellular iron-storage compound in mycelia of Neurospora crassa.
Neurospora crassa produces several structurally distinct siderophores: coprogen, ferricrocin, ferrichrome C and some minor unknown compounds. Under conditions of iron starvation, desferricoprogen is the major extracellular siderophore whereas desferriferricrocin and desferriferichrome C are predominantly found intracellularly. Mössbauer spectroscopic analyses revealed that coprogen-bound iron is rapidly released after uptake in mycelia of the wild-type N. crassa 74A. The major intracellular target of iron distribution is desferriferricrocin. No ferritin-like iron pools could be detected. Ferricrocin functions as the main intracellular iron-storage peptide in mycelia of N. crassa. After uptake of ferricrocin in both the wild-type N. crassa 74A and the siderophore-free mutant N. crassa arg-5 ota aga, surprisingly little metabolization (11%) could be observed. Since ferricrocin is the main iron-storage compound in spores of N. crassa, we suggest that ferricrocin is stored in mycelia for inclusion into conidiospores.
Matzanke
B F
BF
Institut für Biologie I, Universität Tübingen, Federal Republic of Germany.
Bill
E
E
Trautwein
A X
AX
Winkelmann
G
G
D001693:Biological Transport, Active; D005291:Ferrichrome / Q000031:analogs & derivatives* / Q000378:metabolism; D006877:Hydroxamic Acids / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000378:metabolism; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism*; D017262:Siderophores; D015204:Spectroscopy, Mossbauer; D013172:Spores, Fungal / Q000378:metabolism
D006877:Hydroxamic Acids; D007502:Iron Chelating Agents; D017262:Siderophores; D005291:Ferrichrome; C021678:ferricrocin; C010896:coprogen; D007501:Iron