Regulatory proteins (inhibitors or activators) affect estimates of Mr of enzymes and receptors by radiation inactivation. A theoretical model.
The radiation-inactivation method allows the determination of the Mr of enzymes and receptors by monitoring the decay of biological activity as a function of absorbed dose. The presence of regulatory or effector proteins (inhibitors or activators) associated with an enzyme or receptor, or released in the preparation after tissue homogenization, may affect the decay of biological activity. How the activity is affected, however, will depend on the type of inhibition (competitive or non-competitive), the inhibitor or activator concentration, the dissociation constant of the enzyme-effector system, and the effector Mr relative to that of the enzyme. Since little is known on how effector proteins influence radiation inactivation of enzymes and receptors, we have considered a theoretical model in an effort to provide a framework for the interpretation of experimentally obtained data. Our model predicts that competitive and non-competitive inhibitors of enzymes could be distinguished by analysing irradiated samples with various substrate concentrations. Inhibitors will decrease whereas activators will increase the apparent target size of enzymes or receptors.
Potier
M
M
Giroux
S
S
D001667:Binding, Competitive; D003201:Computers; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism* / Q000528:radiation effects; D007700:Kinetics; D008956:Models, Chemical; D011506:Proteins / Q000494:pharmacology*; D011854:Radiochemistry; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000528:radiation effects
D004791:Enzyme Inhibitors; D004798:Enzymes; D011506:Proteins; D011956:Receptors, Cell Surface