IgG binding enhances DNAase I sensitivity of N-acetoxy-N-2-acetylaminofluorene-modified phi X-174 RF DNA.
DNA restriction fragments of phi X-174 RF were modified with the carcinogen, N-acetoxy-N-2-acetylaminofluorene (N-Aco-AAF). Immune complexes of 5'-32P-labeled AAF-modified DNA and rabbit immunoglobulin (IgG) against AAF-guanosine were specifically bound by surface membranes of Cowan I strain micrococci whose protein A binds the Fc portion of IgG. DNAase I sensitivity of the bound DNA was 20-fold greater than in solution, but the normal pattern of hydrolysis was not altered, as determined in sequencing gels. Nonadducted DNA ligated to AAF-modified DNA acquired the enhanced sensitivity to DNAase I hydrolysis when the ligation hybrid was immunobound.
Bases
R
R
Mendez
F
F
Davidson
A
A
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives*; D000099:Acetoxyacetylaminofluorene / Q000494:pharmacology*; D000818:Animals; D000936:Antigen-Antibody Complex; D010584:Bacteriophage phi X 174 / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G; D007700:Kinetics; D010761:Phosphorus Radioisotopes; D011323:Primates; D013205:Staphylococcal Protein A
D000936:Antigen-Antibody Complex; D004279:DNA, Viral; D007074:Immunoglobulin G; D010761:Phosphorus Radioisotopes; D013205:Staphylococcal Protein A; D000099:Acetoxyacetylaminofluorene; D015073:2-Acetylaminofluorene; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I