title: Characterization of beta-adrenergic receptors in fresh and primary cultured bovine corneal endothelium.
abstract: Homogenates of fresh bovine corneal endothelium and of cells from primary cultures exhibited high affinity binding of [3H]-dihydroalprenolol, a specific beta-adrenergic antagonist. The binding was rapid and reversible. Specific binding of the radioligand in each preparation was saturable with half-maximal binding occurring at 0.5 nM. Homogenates of fresh tissue consistently showed a higher maximal binding capacity than did those from cell cultures. Both homogenates bound adrenergic agents in a manner consistent with the labelling of beta-adrenergic receptors. The relative affinities of epinephrine and norepinephrine indicated that beta-adrenergic receptors in both preparations are of the beta 2 subtype. The beta-adrenergic agonist, isoproterenol, increased the cAMP content of intact, cultured endothelial cells 27-fold over control. This effect was completely blocked by the beta-adrenergic antagonist, propranolol. Bovine corneal endothelial cells in primary culture contain beta-adrenergic receptors which are essentially identical to those from in vivo sources and appear to be linked functionally to cAMP synthesis in these cells.
authors.forename: R J
authors.initials: RJ
authors.lastname: Walkenbach
authors.forename: W T
authors.initials: WT
authors.lastname: Chao
authors.forename: D B
authors.initials: DB
authors.lastname: Bylund
authors.forename: S R
authors.initials: SR
authors.lastname: Gibbs
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004082:Dihydroalprenolol / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine