Fanconi's anemia lymphocytes: effect of caffeine, adenosine and niacinamide during G2 prophase.
In this investigation peripheral blood lymphocytes from 3 Fanconi's anemia (FA) patients, 2 FA heterozygotes and 4 normal subjects were treated with caffeine and/or adenosine, and/or niacinamide during G2 prophase. Caffeine dramatically increased breakage levels in homozygote and heterozygote cells. Niacinamide and adenosine decreased the amount of chromosomal aberrations detected in FA homozygote and heterozygote lymphocytes treated and untreated with caffeine during G2 prophase. Caffeine sensitivity of heterozygote lymphocytes is proposed as a new clinical test to explore heterozygosis in individuals of FA families.
Pincheira
J
J
Depto. de Biología Celular y Genética, Facultad de Medicina, Universidad de Chile, Santiago.
Bravo
M
M
López-Sáez
J F
JF
D000241:Adenosine / Q000494:pharmacology*; D000741:Anemia, Aplastic / Q000235:genetics*; D002110:Caffeine / Q000494:pharmacology*; D002869:Chromosome Aberrations; D004260:DNA Repair / Q000187:drug effects*; D005199:Fanconi Anemia / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D007399:Interphase; D008214:Lymphocytes; D009243:NAD / Q000502:physiology; D009536:Niacinamide / Q000494:pharmacology*
D009243:NAD; D009536:Niacinamide; D002110:Caffeine; D000241:Adenosine