title: Storage of spheroplasts at -70 degrees C for transfection with phi X174 RF DNA.
abstract: A method was developed for preparation of spheroplasts used for transfection with phi X174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at -70 degrees C.
authors.affiliation: National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
authors.forename: H V
authors.initials: HV
authors.lastname: Malling
authors.forename: M C
authors.initials: MC
authors.lastname: Fater
authors.forename: J G
authors.initials: JG
authors.lastname: Burkhart
authors.forename: S C
authors.initials: SC
authors.lastname: Hardies
authors.forename: C A
authors.initials: CA
authors.lastname: Hutchison
authors.forename: M H
authors.initials: MH
authors.lastname: Edgell
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics*; D002458:Cell Fractionation; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D005615:Freezing; D013104:Spheroplasts / Q000648:ultrastructure*; D014162:Transfection
chemical_list: D004279:DNA, Viral