Keratan-sulphate-containing proteoglycans in human osteochondrophytic spurs of the femoral head.
Newly synthesized and endogenous proteoglycan was isolated from human femoral head osteochondrophytic spurs. 35SO4-containing keratan sulphate was measured by its susceptibility to endo-beta-D-galactosidase (keratanase) and comprised 15-17% of the two subpopulations of a proteoglycan monomer fraction (D1) resolved by Sepharose CL-2B chromatography (Kav (I), 0.22; (II), 0.78). The size of the newly synthesized keratan sulphate in these fractions was large (Mr greater than 7,000). The hydroxylamine cleavage product of a proteoglycan aggregate fraction (A1) which eluted in the void volume of a Sepharose CL-2B column was immunoreactive with an anti-keratan sulphate monoclonal antibody, 5-D-4. Unlike the proteoglycan aggregate A1 fraction from bovine nasal cartilage, immunoreactivity against 5-D-4 was also found in chromatographic fractions retarded by Sepharose CL-2B. These results lend additional support to our assertion that the osteophyte extracellular matrix consists of hyaline cartilage-type proteoglycan. Stimulation of osteophyte proliferation may be useful as a repair mechanism for resurfacing denuded areas of osteoarthritic femoral heads.
Malemud
C J
CJ
Department of Medicine, Case Western Reserve University, Cleveland, Ohio.
Goldberg
V M
VM
Kresina
T F
TF
D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D002358:Cartilage, Articular / Q000032:analysis / Q000378:metabolism; D002850:Chromatography, Gel; D005270:Femur Head / Q000032:analysis / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D007632:Keratan Sulfate / Q000032:analysis* / Q000276:immunology; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism*; D011509:Proteoglycans / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D006025:Glycosaminoglycans; D011509:Proteoglycans; D007632:Keratan Sulfate