title: Inhibition of hepatic phosphoenolpyruvate carboxykinase by avian reticuloendotheliosis viruses.
abstract: Severe weight loss is associated with many malignant diseases of humans and animals. Avian reticuloendotheliosis viruses (RE viruses) induce runting in experimentally infected chickens. Chickens infected with a replication-competent RE virus, reticuloendotheliosis-associated virus, weighed 30-50% less than control birds at the time of death. Chickens infected with reticuloendotheliosis virus, a replication-defective acute leukemia virus, weighed 30% less than the controls. The runting induced by RE viruses does not occur because of reduced food intake. Activities of phosphoenolpyruvate carboxykinase, a key gluconeogenic enzyme in the liver, were reduced approximately 40 and 50%, respectively, by infection with reticuloendotheliosis-associated virus and reticuloendotheliosis virus. RE virus infection, however, did not affect the hepatic pyruvate carboxylase activity, indicating that inhibition of phosphoenolpyruvate carboxykinase is not due to a general inhibition of all liver enzymes. Birds given injections of UV-inactivated RE viruses or reticuloendotheliosis virus-transformed, non-virus-producing tumor cells also exhibited a reduction in phosphoenolpyruvate carboxykinase activity.
authors.forename: R F
authors.initials: RF
authors.lastname: Garry
authors.forename: G M
authors.initials: GM
authors.lastname: Shackleford
authors.forename: L J
authors.initials: LJ
authors.lastname: Berry
authors.forename: H R
authors.initials: HR
authors.lastname: Bose
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004435:Eating; D006378:Helper Viruses; D008099:Liver / Q000201:enzymology*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism*; D001356:Reticuloendotheliosis virus / Q000528:radiation effects; D014412:Tumor Virus Infections / Q000201:enzymology*; D014779:Virus Replication
chemical_list: D010729:Phosphoenolpyruvate Carboxykinase (GTP)