title: Characterization and physical map of choleraphage phi 149 DNA.
abstract: Choleraphage phi 149 DNA is a linear double-stranded molecule 69 X 10(6) Da or 104 kilobase pairs (kbp). From restriction enzyme analysis, it has been concluded that the DNA is circularly permuted. There are at least three S1 nuclease-sensitive sites along the length of the molecule. These sites represent single-strand interruptions repairable by T4 DNA ligase. A physical map of the DNA has been constructed using the restriction endonucleases BamH1 and BglII.
authors.forename: A
authors.initials: A
authors.lastname: Sengupta
authors.forename: P
authors.initials: P
authors.lastname: Ray
authors.forename: J
authors.initials: J
authors.lastname: Das
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014733:Vibrio
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases