title: alpha 2-Adrenoceptors in dog kidney: autoradiographic localization and putative functions.
abstract: The location of alpha 2-adrenoceptors has been examined in slide-mounted sections of dog kidney by using labelling in vitro with [3H]rauwolscine and autoradiography. [3H]Rauwolscine binding in dog kidney was of high affinity, saturable, and to a single population of sites. Stereoselectivity was observed for (-)-noradrenaline and the relative affinity of a series of competing antagonists indicated binding to alpha 2-adrenoceptors. Autoradiography of [3H]rauwolscine binding revealed localization to three areas: glomeruli, the intimal and adventitial surfaces of arcuate blood vessels and the medullary vascular bundles.
authors.forename: R J
authors.initials: RJ
authors.lastname: Summers
authors.forename: J A
authors.initials: JA
authors.lastname: Stephenson
authors.forename: S
authors.initials: S
authors.lastname: Lipe
authors.forename: C B
authors.initials: CB
authors.lastname: Neylon
mesh_terms: D000818:Animals; D001345:Autoradiography; D004285:Dogs; D007668:Kidney / Q000032:analysis* / Q000098:blood supply / Q000378:metabolism; D007678:Kidney Glomerulus / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism; D011942:Receptors, Adrenergic, alpha / Q000032:analysis*; D015016:Yohimbine / Q000378:metabolism
chemical_list: D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine