Biochemical changes induced by Coxsackie B4 virus in short-term culture of mouse pancreatic islets.
Isolated mouse pancreatic islets were infected in vitro with two strains of Coxsackie B4 virus--a tissue culture-adapted strain and a mouse pancreas-adapted strain. Within 48 h of infection changes had occurred in the biochemical activities of islets infected with the mouse pancreas-adapted strain of virus. Basal insulin release was increased two-fold in these islets, while glucose-induced insulin secretion remained unchanged. Insulin biosynthesis was greatly reduced at a stimulatory concentration of glucose (20 mM), thus leading to a reduced insulin content in these islets. These effects are of importance because they demonstrate that certain strains of Coxsackie B4 virus, like encephalomyocarditis virus, may selectively alter beta-cell function in vitro.
Szopa
T M
TM
Gamble
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DR
Taylor
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KW
D000818:Animals; D003384:Coxsackievirus Infections / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000209:etiology; D029822:Enterovirus B, Human; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010195:Pancreatitis / Q000378:metabolism*
D007328:Insulin; D005947:Glucose