Enhancement of mixed lymphocyte culture reactions between donor and recipient in B cell chronic leukemia using antibody-coated beads.
Matching of donors and recipients in cases of B cell chronic lymphocytic leukemia (CLL) is complicated by the fact that the cancer clone dominates the blood. A case of CLL was characterized for its antigenic phenotype using monoclonal antibodies. Iron beads were coated with the appropriate antibody and used to remove the cancer clone. A mixed lymphocyte culture (MLC) utilizing the purified cells as well as unpurified cells and sheep cell rosetted purified cells was performed with the donor. Using coated beads, the T cell population rose from 8% to 95% in one treatment versus 8% to 42% with sheep cells. MLC data were statistically better with bead-treated cells, and the results demonstrated the importance of this technique for transplantation matching.
Measel
J W
JW
Department of Microbiology and Immunology, Texas College of Osteopathic Medicine, Fort Worth 76017.
Cammarata
P
P
Ferguson
K A
KA
Distefano
A
A
D000906:Antibodies; D005434:Flow Cytometry; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008722:Methods; D010641:Phenotype; D012397:Rosette Formation; D013936:Thymidine / Q000493:pharmacokinetics
D000906:Antibodies; D013936:Thymidine