Antiprostate carcinoma monoclonal antibody (D83.21) cross reacts with a membrane antigen expressed on cytomegalovirus-transformed human fibroblasts.
Virological and epidemiological studies have implicated human cytomegalovirus (HCMV) as a possible etiological agent of prostate cancer. Because of the suspected associations, this laboratory tested the reactivity of a prostate-associated monoclonal antibody with HCMV-transformed cells. This mouse monoclonal antibody, D83.21, reacts with a membrane antigen on prostate and bladder tumor cells and does not bind to a variety of other malignant or normal cells. The results of this study indicated that the prostate-associated antibody bound to a membrane antigen on HCMV-transformed cells as detected by radioimmunoassay, immunofluorescence, and complemented-dependent cytotoxicity. This cross-reactivity appeared to be specific for HCMV-transformed cells and did not react with HCMV-infected cells or those transformed by other viruses. Antibody affinity chromatography, used to isolate the D83.21-reactive protein, revealed two peptides of 60 and 28 kd on both prostate tumor and HCMV-transformed cells. The results suggest that D83.21 reacts with a common cell surface protein expressed on HCMV-transformed cells and urogenital tumors.
Campbell
A E
AE
Beckett
M L
ML
Starling
J J
JJ
Sieg
S M
SM
Wright
G L
GL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000276:immunology*; D005347:Fibroblasts / Q000276:immunology; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D011471:Prostatic Neoplasms / Q000276:immunology*; D011961:Receptors, Fc / Q000032:analysis; D001749:Urinary Bladder Neoplasms / Q000276:immunology
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D011961:Receptors, Fc