title: A regulatory protein for orthophosphate-regulated cyclic phosphodiesterase in Neurospora crassa.
abstract: A regulatory protein for orthophosphate-regulated cyclic phosphodiesterase (cPDase) was detected in mycelial extracts of Neurospora crassa. The protein, designated neucrassin, was precipitated by ammonium sulfate between 60 to 100% saturation, and fractionated by gel filtration through a TSK-gel column. The molecular weight was estimated to be 65,000. Neucrassin inhibited the hydrolyzing activity of cPDase for cyclic 3',5'-AMP in the presence of MnCl2 in a noncompetitive manner, whereas it stimulated the activity for cyclic 2',3'-AMP over a wide ranges of pH's, between 2.8 to 6.8.
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.forename: K
authors.initials: K
authors.lastname: Hasunuma
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000302:isolation & purification* / Q000378:metabolism; D002413:Cations, Divalent; D004533:Egtazic Acid / Q000494:pharmacology; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D002413:Cations, Divalent; D005656:Fungal Proteins; C046269:neucrassin, Neurospora; D004533:Egtazic Acid; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases