T cell blast stimulation of MLR: role of interleukin 1 and interleukin 2.
T cells blasts were examined for the ability to stimulate primary mixed leucocyte reactions (MLR) in the presence or absence of supplemental interleukin 1 (IL-1) or interleukin 2 (IL-2). After purification, resting T cells were activated using monoclonal anti-CD3 antibody in an accessory-cell free systems. After irradiation, such cells were found to be non-stimulatory in autologous or allogeneic MLR except in the presence of supplemental IL-2. IL-1 was an ineffective cofactor, despite its ability to enhance anti-CD3 mediated stimulation of T cells. It is concluded that T cell blasts may lack an essential factor needed to stimulate IL-2 secretion (other than IL-1), or may elicit responses only from allogeneic T cells not bearing IL-1 receptors.
Aaron
S
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Department of Medicine, University of Alberta, Canada.
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D002454:Cell Differentiation; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D007959:Lymphocyte Culture Test, Mixed; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D006684:HLA-DR Antigens; D007375:Interleukin-1; D007376:Interleukin-2