Lectin (UEA-1) reaction of capillary endothelium with reference to permeability in autopsied cases of cerebral infarction.
The relationship between endothelial reactivity to Ulex europaeus agglutinin-1 (UEA-1) and the permeability of the vascular wall in human autopsied cases of cerebral infarction was studied. Sections from the cerebral cortex were reacted with horseradish peroxidase UEA-1 to demonstrate the surface membrane of endothelial cells. Albumin in the neuropil of sections was demonstrated for the estimation of increased vascular permeability. The results showed that endothelial reactivity to UEA-1 was reduced in cases where death had occurred 3 to 5 days after onset of cerebral infarction. Reactivity was also diminished in cases where death had occurred after 13 and 25 days; these cases showed fresh ischemic lesions caused by re-attacks of infarction. Albumin extravasation into the neuropil was demonstrated in these intermediate cases. Chronic cases, dying after more than 52 days, showed no reduction of endothelial reactivity to UEA-1 and no albumin extravasation was proved. It was concluded that UEA-1 can be employed as a useful morphological marker for evaluation of endothelial function and vascular permeability.
Kadota
E
E
Second Department of Pathology, Kinki University School of Medicine, Osaka, Japan.
Tanji
K
K
Nishida
S
S
Takahashi
M
M
Maeda
M
M
Hiruma
S
S
Enomoto
Y
Y
Hashimoto
S
S
Akai
F
F
D000368:Aged; D000369:Aged, 80 and over; D001344:Autopsy; D002199:Capillary Permeability; D002544:Cerebral Infarction / Q000378:metabolism / Q000473:pathology*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins; D008297:Male; D037121:Plant Lectins; D012709:Serum Albumin / Q000032:analysis
D037102:Lectins; D037121:Plant Lectins; D012709:Serum Albumin; C036850:Ulex europaeus lectins