Cloning and sequence analysis of a cDNA encoding the beta-subunit of mouse beta-hexosaminidase.
A cDNA encoding the prepro-beta-polypeptide of mouse beta-hexosaminidase (Hex) was isolated from a mouse lymphoblast cDNA library. The cDNA contains an open reading frame corresponding to a polypeptide of 536 amino acids which shows 74% homology with the human prepro-beta-polypeptide. An examination of the amino acid sequence identifies a putative signal peptide and five possible glycosylation sites, two of which are identical to the confirmed glycosylation sites of the human beta-chain. The amino acid sequence also shows a structurally similar though not identical site for internal cleavage responsible for the generation of mature beta a- and beta b-polypeptides.
Bapat
B
B
Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
Ethier
M
M
Neote
K
K
Mahuran
D
D
Gravel
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005796:Genes; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics*
D046911:Macromolecular Substances; D004247:DNA; D001619:beta-N-Acetylhexosaminidases