A new 5'-nucleotidase inhibitor, nucleoticidin. I. Taxonomy, fermentation, isolation and biological properties.
A new 5'-nucleotidase inhibitor named nucleoticidin was produced by Pseudomonas sp. YM-3229G. It was isolated from a fermentation broth by trichloroacetic acid extraction, ethanol precipitation and Dowex 1 and DEAE-52 column chromatography. It inhibited 5'-nucleotidase activity of snake venom and rat liver membrane. It also showed antitumor activity against solid type Sarcoma 180.
Ogawara
H
H
Uchino
K
K
Akiyama
T
T
Watanabe
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D005285:Fermentation; D008099:Liver / Q000201:enzymology; D009708:Nucleotidases / Q000037:antagonists & inhibitors*; D011549:Pseudomonas / Q000378:metabolism; D051381:Rats; D012513:Sarcoma, Experimental / Q000378:metabolism; D012910:Snake Venoms / Q000037:antagonists & inhibitors; D012988:Soil Microbiology
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D012910:Snake Venoms; D009708:Nucleotidases; C030287:nucleotidase