title: Two simultaneously active VSG gene transcription units in a single Trypanosoma brucei variant.
abstract: Trypanosomes can change their surface coat either by slotting a different surface antigen gene copy into an active (telomeric) expression site or by activating a new VSG gene expression site and inactivating the old one. How expression sites are activated or inactivated is not clear. We report an exceptional trypanosome variant in which the inactivation of a surface antigen gene is accompanied by a 30 kb DNA insertion 5' of the gene. Transcription of the region upstream of the insertion continues unaltered and retains the characteristic insensitivity to alpha-amanitin of VSG gene transcription units, showing that the expression site is still active. The expressed VSG gene in this trypanosome variant resides in another telomere. Hence, two VSG gene transcription units can be simultaneously active. This argues against a single mobile activating element controlling VSG gene transcription and favors a stochastic model of telomere activation/inactivation.
authors.forename: A W
authors.initials: AW
authors.lastname: Cornelissen
authors.forename: P J
authors.initials: PJ
authors.lastname: Johnson
authors.forename: J M
authors.initials: JM
authors.lastname: Kooter
authors.forename: L H
authors.initials: LH
authors.lastname: Van der Ploeg
authors.forename: P
authors.initials: P
authors.lastname: Borst
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D009154:Mutation; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D014643:Variant Surface Glycoproteins, Trypanosoma
chemical_list: D004251:DNA Transposable Elements; D006023:Glycoproteins; D008565:Membrane Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma