A comparison of the genomes of bovine herpesvirus type 1 and pseudorabies virus.
The DNA sequence homology between bovine herpesvirus type 1 (BHV-1) and pseudorabies virus (PRV) was examined. Reciprocal cross-hybridization of viral DNA labelled by nick translation to Southern blots of restriction endonuclease-digested DNA detected homologous sequences dispersed throughout the genomes of the two viruses. The DNA-DNA hybrids formed were stable under high-stringency wash conditions. Sequences of a 32P-labelled PRV DNA fragment probe were found to hybridize only to a specific region of the BHV-1 genome, suggesting that the detected sequence homology was not due to fortuitous hybridization of G + C-rich sequences. As measured by liquid reassociation kinetics the homology between these two viruses was approximately 8%.
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D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification; D005814:Genes, Viral; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D007668:Kidney; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D011817:Rabbits; D013045:Species Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes