Spectroscopic characterization of vesicle formation on heated human erythrocytes and the influence of the antiviral agent amantadine.
EPR investigations on the vesiculation process of heated human erythrocytes were performed, using different fatty acid spin labels. Spectrin denaturation and vesiculation do not influence the fluidity of the lipid phase of the remaining membrane of human erythrocytes: Vesicles released differ in chemical composition as well as in the lipid fluidity of their membrane from the intact human erythrocyte membrane. A reduced cholesterol-to-phospholipid ratio and a depletion of spectrin was found. By changing the ionic concentration of the suspension medium an effect on membrane spectra and on vesicle release was established. The adamantane derivative amantadine causes fluidization of the human erythrocyte membrane and inhibits vesicle release. Based on these results, a model for the mechanism by which adamantane-like molecules could interact with membranes is proposed.
Herrmann
A
A
Lentzsch
P
P
Lassmann
G
G
Ladhoff
A M
AM
Donath
E
E
D000547:Amantadine / Q000494:pharmacology*; D002784:Cholesterol / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects*; D005227:Fatty Acids / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D010743:Phospholipids / Q000032:analysis; D011489:Protein Denaturation; D013049:Spectrin / Q000032:analysis; D013113:Spin Labels / Q000378:metabolism
D005227:Fatty Acids; D010743:Phospholipids; D013113:Spin Labels; D013049:Spectrin; D002784:Cholesterol; D000547:Amantadine