Use of OKT3 hybridoma cells to clonally activate CD3+ human T lymphocytes.
A simple and reliable method was developed to induce clonal growth of resting human T cells. In this limiting dilution (LD) culture system, responder cells (unseparated mononuclear cells, E rosette-purified T cells, or cell sorter-separated CD4+ and CD8+ subsets) were activated by irradiated anti-CD3-secreting (OKT3) hybridoma cells in the presence of exogenous IL-2 (crude culture supernatant or recombinant IL-2). Under these conditions, one out of 2-3 CD4+ and CD8+ T cells developed into a proliferating cell clone. Addition of recombinant IL-1 slightly enhanced the growth frequency and increased the clone size of CD4+ cells but did not affect the growth pattern of CD8+ cells.
Kabelitz
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Department of Medical Microbiology and Immunology, University of Ulm, F.R.G.
Janssen
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Brucker
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D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D017252:CD3 Complex; D002469:Cell Separation; D002478:Cells, Cultured; D004261:DNA Replication; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D011994:Recombinant Proteins / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D007376:Interleukin-2; D011994:Recombinant Proteins