Radiolabeling of the interferon-alpha receptor.
The action of alpha interferon (IFN-alpha) is initiated by its binding to a specific cell-surface glycoprotein, the IFN-alpha receptor, which is not well characterized. IFN-alpha A was reacted with an 125I-labeled, cleavable, heterobifunctional reagent. The derivatized IFN-alpha A was bound to human Daudi cells and photoactivated, forming a covalent IFN/receptor complex of apparent molecular weight 130,000-140,000 by SDS-polyacrylamide gel electrophoresis. Cleavage of the complex produced a new 125I-labeled 110 kDa band, representing the 125I-labeled IFN-alpha receptor free of IFN-alpha. This result provides a better estimate of the apparent molecular weight of the IFN-alpha receptor, and also provides a tool for tracking the migration of the free receptor in SDS-polyacrylamide gel electrophoresis.
Langer
J A
JA
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854-5635.
D000345:Affinity Labels; D001386:Azides; D002460:Cell Line; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D008970:Molecular Weight; D010777:Photochemistry; D011971:Receptors, Immunologic / Q000378:metabolism; D017471:Receptors, Interferon; D011994:Recombinant Proteins
D000345:Affinity Labels; D001386:Azides; D003432:Cross-Linking Reagents; D007370:Interferon Type I; D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins; C042649:N-(4-(4-azido-3-iodophenylazo)benzoyl)-3-aminopropyl-N'-oxysulfosuccinimide ester