title: Plasma cell antigen PC-1 and the transferrin receptor in mouse, rat, and hamster: serologic and biochemical analysis.
abstract: The plasma cell membrane antigen PC-1 and the receptor for the iron transport protein transferrin are high m.w., developmentally regulated proteins consisting of two similar or identical disulfide-bonded subunits. In this paper, we report the results of a serologic and biochemical analysis of these proteins in various strains of inbred mice, and in rats and hamsters. A monoclonal antibody against the PC-1a allelic product is shown to detect an antigenic determinant on the PC-1 molecule that has the same strain distribution as the antigen previously detected with polyclonal alloantisera. The mouse PC-1 protein was purified from plasma cells of the PC-1a genotype and was used to generate polyclonal rabbit anti-PC-1 antibodies. These antibodies precipitated a homologous protein from plasmacytoma cells derived from PC-1- congenic mice, demonstrating that PC-1b is not a "null" allele. The PC-1b allelic product had a slightly lower apparent m.w. than the PC-1a product, and had a slightly more basic isoelectric point. Rabbit anti-mouse PC-1 antibodies also precipitated a homologous protein from immunoglobulin-secreting cells of rat and hamster origin, but did not show detectable cross-reaction with the transferrin receptor. Disulfide bonding between chains was conserved in both PC-1 and the transferrin receptor in all species examined, but transferrin receptors from mouse cells had a significantly higher apparent m.w. than those of rat, hamster, or human cells.
authors.forename: I R
authors.initials: IR
authors.lastname: van Driel
authors.forename: J W
authors.initials: JW
authors.lastname: Goding
authors.forename: N
authors.initials: N
authors.lastname: Koch
mesh_terms: D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics; D006224:Cricetinae; D004721:Endoplasmic Reticulum / Q000276:immunology; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009194:Myeloma Proteins / Q000032:analysis* / Q000235:genetics; D010802:Phylogeny; D010950:Plasma Cells / Q000276:immunology* / Q000378:metabolism; D010954:Plasmacytoma / Q000276:immunology; D011498:Protein Precursors / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics; D011990:Receptors, Transferrin
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D008565:Membrane Proteins; D009194:Myeloma Proteins; D011498:Protein Precursors; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin