Monoclonal antibody AHN-1 inhibits phagocytosis by human neutrophils.
The granulocyte-specific monoclonal antibody, AHN-1, immunoprecipitates two major surface-iodinated proteins of 105,000 and 145,000 to 150,000 daltons from normal human neutrophils. In this study, the effect of AHN-1 on a number of neutrophil functions was evaluated in vitro. Both complement- and antibody-mediated phagocytosis were inhibited when human neutrophils were pretreated with AHN-1 and opsonized bacteria were used as targets. The inhibition of phagocytosis was specific, in that lysosomal enzyme release and chemotaxis were not altered by treatment with AHN-1. AHN-1 did inhibit superoxide production by neutrophils in response to particulate stimuli, but not in response to the soluble stimulus, 12-O-tetradecanoylphorbol-13-acetate. The data indicate that one or both of these surface proteins may be important in the process of phagocytosis. AHN-1 should be useful in isolating and further characterizing the nature of these molecules.
Skubitz
K M
KM
Weisdorf
D J
DJ
Peterson
P K
PK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002457:Cell Extracts / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D003594:Cytoplasmic Granules / Q000201:enzymology; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D010587:Phagocytosis; D013212:Staphylococcus epidermidis / Q000276:immunology; D013481:Superoxides / Q000097:blood
D000911:Antibodies, Monoclonal; D002457:Cell Extracts; D013481:Superoxides