title: Spin-labeled erythrocyte membranes: direct identification of nitroxide-conjugated proteins.
abstract: The covalent incorporation of a spin-labeled analog of N-ethyl maleimide into erythrocyte membrane proteins has been monitored by electron paramagnetic resonance spectroscopy and the individually labeled proteins detected by immunoblotting techniques, using an anti-nitroxide antibody, following electrophoretic separation of the membrane components. Spin-label was primarily found in the high molecular weight bands (I and II) with no incorporation in proteins with molecular weights less than 35,000. Increasing the reaction time between the spin-label and ghosts altered both the observed labeling pattern and the epr spectra with an increase in the proportion of strongly-immobilized species.
authors.forename: M J
authors.initials: MJ
authors.lastname: Barber
authors.forename: L P
authors.initials: LP
authors.lastname: Solomonson
authors.forename: D C
authors.initials: DC
authors.lastname: Eichler
mesh_terms: D003497:Cyclic N-Oxides / Q000097:blood*; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008565:Membrane Proteins / Q000097:blood*; D008970:Molecular Weight; D013113:Spin Labels
chemical_list: D003497:Cyclic N-Oxides; D008565:Membrane Proteins; D013113:Spin Labels; C017480:4-maleimido-2,2,6,6-tetramethylpiperidinooxyl