title: Molecular organisation of the quinic acid utilization (QUT) gene cluster in Aspergillus nidulans.
abstract: The functional integrity of the QUTB gene (encoding quinate dehydrogenase) has been confirmed by transformation of a qutB mutant strain. The DNA sequence of the contiguous genes QUTD (quinate permease), QUTB and QUTG (function unknown) has been determined and analysed, together with that of QUTE (catabolic 3-dehydroquinase). The QUTB sequence shows significant homology with the shikimate dehydrogenase function of the complex AROM locus of Aspergillus nidulans, and with the QA-3 quinate dehydrogenase and QA-1S (repressor) genes of Neurospora crassa. The QUTD gene shows strong homology with the N. crassa QA-Y gene and QUTG with the QA-X gene. QUTD, QUTB, and QUTG, QUTE form two pairs of divergently transcribed genes, and conserved sequence motifs identified in the two common 5' non-coding regions show significant homology with UASGAL and UASQA sequences of the Saccharomyces cerevisiae and N. crassa Gal and QA systems. In addition, conserved 5' sequences homologous to the mammalian CAAT box are noted and a previously unreported conserved 22 nucleotide motif is presented.
authors.affiliation: Department of Genetics, University of Newcastle upon Tyne, UK.
authors.forename: A R
authors.initials: AR
authors.lastname: Hawkins
authors.forename: H K
authors.initials: HK
authors.lastname: Lamb
authors.forename: M
authors.initials: M
authors.lastname: Smith
authors.forename: J W
authors.initials: JW
authors.lastname: Keyte
authors.forename: C F
authors.initials: CF
authors.lastname: Roberts
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000483:Alleles; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D005796:Genes; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics; D011801:Quinic Acid / Q000378:metabolism*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011801:Quinic Acid; D000429:Alcohol Oxidoreductases; C022511:quinate dehydrogenase