title: Shedding and synthesis de novo of Fc and C3b receptors by cultured guinea-pig macrophages.
abstract: Resident macrophages freshly obtained from the peritoneal cavity of guinea-pigs were demonstrated to form a higher percentage of Fc and C3b rosettes than elicited macrophages when low concentrations of IgG and IgM-C3b were used to sensitize ox red blood cells (ORBC) in rosette assays. Culture of the total resident and elicited macrophages for 6 h at 37 degrees C resulted in a decrease of Fc and C3b rosette-forming cells, the loss of Fc receptor-bearing cells by resident macrophages only being apparent when using a sub-optimal concentration of sensitizing IgG. After 24 h incubation the percentages of Fc and C3b rosettes returned to their initial values. In contrast, there was no decline in the percentage of Fc and C3b rosettes formed by the adherent population of resident and elicited macrophages cultured for 6 h. However, extending the incubation of the adherent macrophage to 24 h produced an increase of Fc receptor-positive cells and a dramatic decrease of C3b receptor-positive cells. Culture supernatants of the total macrophage population that had been incubated for 6 h inhibited Fc and C3b rosette formation by freshly obtained elicited macrophages. These results, together with the demonstration that treatment of the total macrophage population with cycloheximide led to an inhibition of Fc and C3b receptor expression after 24 h culture, suggest that the Fc and C3b receptors of guinea-pig macrophages are shed and synthesized de novo during short-term culture. This system could be applied to the study in vitro of soluble immunoregulatory mediators on macrophage functions which are dependent on the expression of Fc and C3b receptors.
authors.affiliation: Department of Immunology, Rayne Institute, United Medical School, St Thomas's Hospital, London, UK.
authors.forename: G A
authors.initials: GA
authors.lastname: Limb
authors.forename: K A
authors.initials: KA
authors.lastname: Brown
authors.forename: R A
authors.initials: RA
authors.lastname: Wolstencroft
authors.forename: D C
authors.initials: DC
authors.lastname: Dumonde
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003179:Complement C3b / Q000276:immunology; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D011951:Receptors, Complement / Q000096:biosynthesis*; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000096:biosynthesis*; D012397:Rosette Formation; D013997:Time Factors
chemical_list: D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc; D003179:Complement C3b; D003513:Cycloheximide