Alkaline phosphatase activity can interfere with the enzymatic determination of triglycerides.
Unlike human plasma, rat plasma was found to contain substantial quantities of alkaline phosphatase. The large amount of phosphatase activity in rat plasma interferes with assay methods that employ orthophosphoric acid esters as substrates or reaction intermediates. Although these methods are effective when applied to human plasma samples, they cannot be used with rodent plasma.
Dombrowski
G J
GJ
Swiatek
K R
KR
Chao
K L
KL
D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D006801:Humans; D007202:Indicators and Reagents; D051379:Mice; D009243:NAD; D009244:NAD+ Nucleosidase / Q000097:blood; D051381:Rats; D013053:Spectrophotometry / Q000379:methods; D014280:Triglycerides / Q000097:blood*
D007202:Indicators and Reagents; D014280:Triglycerides; D009243:NAD; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase; D009244:NAD+ Nucleosidase