Regulator soluble factors of human IgE synthesis: II. Biological activity.
In view of the experiments carried out on rats by various investigators in order to identify and characterize soluble factors that regulate IgE synthesis, we decided to apply these systems to humans through different "in vitro" techniques, and in different population groups. After its production through bidirectional mixed cultures as well as its isolation by column of Sepharose Con-A (affinity chromatography), we proceeded to confirm its biological activity. The modifications produced by these factors on the synthesis of IgE "in vitro" (modification of Phadebast IgE PRIST radioimmunoanalysis technique for low levels) on lymphocytes of controls, atopics and pollinosis with and without immunotherapy were confirmed, as well as the non-modification on IgG synthesis. A considerable increase (greater than 300%) in IgE production in lymphocytes of healthy subjects was observed in relation with the stimulating factor obtained in CML of atopic lymphocytes.
Tabar
A I
AI
Department of Allergology, Faculty of Medicine, University of Navarra, Pamplona, Spain.
Sanz
M L
ML
Gamboa
P M
PM
Oehling
A
A
D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000378:metabolism*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*
D006023:Glycoproteins; D007073:Immunoglobulin E