title: [Establishment and characterization of human pancreatic adenocarcinoma cell line JHP-1 producing carbohydrate antigen 19-9 and carcinoembryonic antigen].
abstract: A new tumor cell line derived from the ascites of a patient with adenocarcinoma of the head of pancreas was established in culture and the nude mouse. The cell line was characterized by the growth with a population doubling time of 22 hr., a high plating efficiency on the plastic surface and a modal chromosome number of 66. The tumorigenicity was proved by the growth in nude mouse and in soft agar. Morphologically the cell line grew as a confluent monolayer with tight adhesion to the plastic surface. Histologically the cell line was epithelial-like in culture and poorly differentiated adenocarcinoma in nude mouse. Ultrastructurally the cell line showed a characteristic pancreatic epithelium. Furthermore, the cell line expressed carbohydrate antigen 19-9 and carcinoembryonic antigen. This cell line, designated JHP-1, has been cultured for at least 100 passages in vitro and maintained for more than 2 years. This cell line would be used as a new model for human pancreatic carcinoma.
authors.affiliation: First Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sujino
authors.forename: S
authors.initials: S
authors.lastname: Nagamori
authors.forename: K
authors.initials: K
authors.lastname: Fujise
authors.forename: S
authors.initials: S
authors.lastname: Hasumura
authors.forename: S
authors.initials: S
authors.lastname: Homma
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: M
authors.initials: M
authors.lastname: Niiya
authors.forename: H
authors.initials: H
authors.lastname: Kameda
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D000368:Aged; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis*; D002455:Cell Division; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen