Pertussis toxin inhibits chemotactic factor-induced phospholipase C stimulation and lysosomal enzyme secretion in rabbit neutrophils.
Pertussis toxin suppressed [32P]polyphosphoinositide breakdown and lysosomal enzyme secretion induced by fMet-Leu-Phe in rabbit neutrophils. Likewise, fMet-Leu-Phe- or leukotriene B4-evoked [3H]inositol trisphosphate accumulation was inhibited by the toxin. These findings, taken together with evidence that pertussis toxin specifically causes inactivation of the guanine nucleotide binding protein (Ni), suggests that guanine nucleotide binding proteins may mediate coupling between calcium-mobilising receptors and phospholipase C-mediated reactions in rabbit neutrophils.
Bradford
P G
PG
Rubin
R P
RP
D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D007975:Leukotriene B4 / Q000494:pharmacology; D008247:Lysosomes / Q000201:enzymology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D037342:Pertussis Toxin; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D011817:Rabbits; D010738:Type C Phospholipases / Q000378:metabolism*; D010566:Virulence Factors, Bordetella
D001427:Bacterial Toxins; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010566:Virulence Factors, Bordetella; D007975:Leukotriene B4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D037342:Pertussis Toxin; D010740:Phospholipases; D010738:Type C Phospholipases; D004121:Dimethyl Sulfoxide