title: Neoplastic transformation of mast cells by Abelson-MuLV: abrogation of IL-3 dependence by a nonautocrine mechanism.
abstract: Normal mast cells can be propagated in culture when medium is supplemented with interleukin-3 (IL-3). We demonstrate that Abelson-MuLV (Ab-MuLV) infection of mast cells eliminates dependence on IL-3 for growth. By contrast, Harvey, BALB, and Moloney MSV, which also productively infect mast cells, are unable to relieve IL-3 dependence. Ab-MuLV-induced IL-3-independent lines express the v-abl-specific transforming protein and have phenotypic characteristics of mast cells. These cells also possess high cloning efficiencies in soft agarose and are tumorigenic in nude mice. In addition, Ab-MuLV induces transplantable mastocytomas in pristane-primed adult mice resistant to lymphoid transformation, defining a new hematopoietic target for malignant transformation by this virus. None of the Ab-MuLV-derived transformants express or secrete detectable levels of IL-3 nor is their growth inhibited by anti-IL-3 serum. These results argue that Ab-MuLV abrogation of the IL-3 requirement is not due to an autocrine mechanism.
authors.forename: J H
authors.initials: JH
authors.lastname: Pierce
authors.forename: P P
authors.initials: PP
authors.lastname: Di Fiore
authors.forename: S A
authors.initials: SA
authors.lastname: Aaronson
authors.forename: M
authors.initials: M
authors.lastname: Potter
authors.forename: J
authors.initials: J
authors.lastname: Pumphrey
authors.forename: A
authors.initials: A
authors.lastname: Scott
authors.forename: J N
authors.initials: JN
authors.lastname: Ihle
mesh_terms: D000011:Abelson murine leukemia virus / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002999:Clone Cells; D007377:Interleukin-3; D009052:Leukemia Virus, Murine / Q000502:physiology*; D008099:Liver; D008222:Lymphokines / Q000096:biosynthesis / Q000494:pharmacology*; D008407:Mast Cells / Q000378:metabolism / Q000382:microbiology* / Q000502:physiology; D012515:Mast-Cell Sarcoma / Q000209:etiology; D051379:Mice; D008815:Mice, Inbred Strains; D011971:Receptors, Immunologic / Q000032:analysis; D016185:Receptors, Interleukin-3; D014764:Viral Proteins / Q000032:analysis
chemical_list: D007377:Interleukin-3; D008222:Lymphokines; D011971:Receptors, Immunologic; D016185:Receptors, Interleukin-3; D014764:Viral Proteins