Plasma levels of alpha-atrial natriuretic peptide in normal subjects under various postural conditions.
The present paper describes a radioimmunoassay, in conjunction with an extraction procedure on octadecasilyl silica cartridges, of alpha-atrial natriuretic peptide in human plasma and its application in normal subjects under various postural conditions. The sensitivity of this assay is 1.96 pg/ml. The plasma concentrations of alpha-atrial natriuretic peptide in 18 normal male subjects ranged from 10 to 55 pg/ml. This assay was shown to be reproducible as indicated by the low within- and between-assay coefficients of variation. The plasma alpha-ANP concentration decreased during prolonged storage of the plasma at -20 degrees C or -80 degrees C and its level is also lower in hemolyzed plasma samples. The plasma alpha-ANP concentration is similar in standing and supine position but rose significantly when the subjects were tilted 20 degrees head-down.
Lijnen
P
P
Department of Pathophysiology, University of Leuven, Belgium.
M'Buyamba-Kabangu
J R
JR
Huysecom
J
J
Fagard
R
R
Staessen
J
J
Amery
A
A
D000450:Aldosterone / Q000097:blood; D009320:Atrial Natriuretic Factor / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D011187:Posture; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012083:Renin / Q000097:blood; D013696:Temperature
D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D012083:Renin