[Functional and specific vasoactive intestinal peptide receptors in enterocytes isolated from fetal or adult rats].
Functional and specific VIP receptors (relative potencies: VIP greater than PHI greater than secretin) have been characterized (cAMP generation) in enterocytes isolated from rat fetuses at 19 days gestation, after incubation at 37 degrees C in the presence of IBMX. In fetuses, VIP is about 6 times more potent (EC50 = 2.5 X 10(-10) M) than in adults (EC50 = 15 X 10(-10) M). This difference is not observed for PGE2, and is not related to cAMP-PDE activities (Km, Vmax). It is suggested that VIP may regulate the differentiation and function of enterocytes during the fetal life in rats.
Chastre
E
E
Gespach
C
C
Rosselin
G
G
Broer
Y
Y
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000818:Animals; D005260:Female; D007422:Intestines / Q000166:cytology* / Q000196:embryology / Q000201:enzymology; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000502:physiology; D018005:Receptors, Vasoactive Intestinal Peptide
D011956:Receptors, Cell Surface; D018005:Receptors, Vasoactive Intestinal Peptide; D015105:3',5'-Cyclic-AMP Phosphodiesterases
Récepteurs fonctionnels et spécifiques du peptide intestinal vasoactif dans les entérocytes isolés chez le Rat à l'âge foetal ou adulte.