An A-chain ricin immunotoxin targeted against rat class II major histocompatibility complex molecules. In vitro and in vivo effects.
Studies reported here have demonstrated that an antirat class II major histocompatibility complex molecule A-chain ricin immunotoxin could specifically remove, in a dose-dependent manner, the cells capable of stimulating the rat mixed lymphocyte reaction, an in vitro model of transplant rejection. It was further demonstrated that administration of a monoclonal antibody against class II major histocompatibility complex molecules to isolated rat pancreas grafts by hypothermic perfusion resulted in the targeting of cells bearing class II major histocompatibility complex molecules. But administration of the immunotoxin to isolated pancreases in a similar manner did not prolong their survival when allografted across a major histocompatibility barrier.
Wiley
K N
KN
Urology/Transplantation Laboratories, Royal Hallamshire Hospital, Sheffield, England.
Powell
C S
CS
Henry
L
L
Clark
A
A
Fox
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000502:physiology*; D001667:Binding, Competitive; D005260:Female; D006085:Graft Survival; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D000922:Immunotoxins / Q000008:administration & dosage / Q000494:pharmacology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016035:Pancreas Transplantation; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012276:Ricin / Q000008:administration & dosage / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D000922:Immunotoxins; D012276:Ricin