title: Localization of ganglioside GM1 on myelin, in dissociated cells from rat embryonic cerebral hemispheres, using biotinylated choleragen and avidin peroxidase.
abstract: The distribution of ganglioside GM1 in primary cultured cells from rat cerebral hemispheres was studied. These dissociated cell cultures are capable of forming myelin. 2',3'-Cyclic nucleotide 3'-phosphodiesterase (CNPase) activity, which is considered to be a good marker for myelin, increased with the onset of myelin formation. Ganglioside GM1 was localized on the myelin-related structures using a biotinylated choleragen and avidin peroxidase technique. The distribution of ganglioside GM1 resembled that of immunostained CNPase.
authors.forename: H
authors.initials: H
authors.lastname: Asou
authors.forename: N
authors.initials: N
authors.lastname: Mutou
authors.forename: S
authors.initials: S
authors.lastname: Hirano
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000818:Animals; D001360:Avidin; D001369:Axons / Q000378:metabolism; D001710:Biotin; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002772:Cholera Toxin; D004622:Embryo, Mammalian; D005677:G(M1) Ganglioside / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D009186:Myelin Sheath / Q000378:metabolism*; D051381:Rats
chemical_list: D005732:Gangliosides; D001360:Avidin; D005677:G(M1) Ganglioside; D001710:Biotin; D002772:Cholera Toxin; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases