title: [Effects of pepstatin A on neutrophils; cross-deactivation with FMLP].
abstract: The ability of pepstatin A, a protease inhibitor produced by Streptomyces testaceus, to elicit a number of responses by the human PMN has been studied. In lysozyme and beta-glucuronidase release, pepstatin A 10(-5)M is equivalent to the synthetic oligopeptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) 10(-7)M. In superoxide release, pepstatin A 10(-5)M produces 80% of that originated by FMLP 10(-7). After two minutes of incubation the superoxide release is important, there being no further increase after 10 minutes. Preincubation of the cells with cytochalasin B before stimulation with pepstatin A elicits a noticeable increase in O2- release. In chemotaxis, pepstatin A 10(-6) originates the same cell motility as FMLP 10(-9). Pepstatin A produces a cross deactivation with FMLP which adds further evidence to the hypothesis that both stimuli compete for the same receptor in the PMN.
authors.forename: M A
authors.initials: MA
authors.lastname: Muniain
authors.forename: F
authors.initials: F
authors.lastname: Pozuelo
authors.forename: J J
authors.initials: JJ
authors.lastname: Pérez-Venegas
authors.forename: A
authors.initials: A
authors.lastname: Romero-Tabares
authors.forename: M C
authors.initials: MC
authors.lastname: Rodríguez-Hornillo
authors.forename: M
authors.initials: M
authors.lastname: Garrido
mesh_terms: D001667:Binding, Competitive; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology; D003574:Cytochrome c Group / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D009113:Muramidase / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D009842:Oligopeptides / Q000494:pharmacology*; D010436:Pepstatins / Q000494:pharmacology*; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism
chemical_list: D003574:Cytochrome c Group; D009842:Oligopeptides; D010436:Pepstatins; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D013481:Superoxides; C014195:Streptomyces pepsin inhibitor; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009113:Muramidase; D005966:Glucuronidase; C031375:pepstatin
vernacular_title: Efectos de la pepstatina A sobre los neutrófilos; desactivación cruzada con el FMLP.