The effect of bezafibrate and long-chain fatty acids on peroxisomal activities in cultured rat hepatocytes.
Peroxisomal activities have been evaluated in cultured rat hepatocytes in the presence of bezafibrate or long-chain fatty acids added to the culture medium. All activities decreased continuously over a time period of 100 h in culture but selected activities were relatively increased as a function of the added effectors. This relative increase in peroxisomal activities was dose-dependent, discernible within the first 24 h in culture and consisted of activities related specifically to peroxisomal fatty acyl beta-oxidation, e.g., cyanide-insensitive palmitoyl-CoA oxidation, H2O2-forming palmitoyl-CoA oxidase and heat-labile enoyl-CoA hydratase. Peroxisomal catalase or mitochondrial fatty acyl beta-oxidation (cyanide-sensitive) remained relatively unchanged. The relative increase in peroxisomal activities was accompanied by a respective increase in the number of peroxisomes as well as in thymidine incorporation rate.
Hertz
R
R
Arnon
J
J
Bar-Tana
J
J
D000818:Animals; D001629:Bezafibrate / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D014503:Urate Oxidase / Q000378:metabolism
D005227:Fatty Acids; D014280:Triglycerides; D010171:Palmitoyl Coenzyme A; D002374:Catalase; D014503:Urate Oxidase; D005952:Glucose-6-Phosphatase; D004746:Enoyl-CoA Hydratase; D013936:Thymidine; D001629:Bezafibrate