title: Construction of a cosmid clone library of Pseudomonas syringae pv. phaseolicola and isolation of genes by functional complementation.
abstract: A genomic library constructed from a wild-type strain of Pseudomonas syringae pv. phaseolicola in the broad-host-range cosmid vector pVK102 was used to isolate wild-type genes by complementation of Tn5-induced auxotrophic mutants. Selection pressure was required for maintenance of the vector and members of the library in strains of P. syringae.
authors.forename: M
authors.initials: M
authors.lastname: Ehrenshaft
authors.forename: D
authors.initials: D
authors.lastname: Mills
mesh_terms: D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements