Characterization of ouabain-resistant mutants of the preadipocyte Ob17 clonal line. Adipose conversion in vitro and in vivo.
After exposure to a mutagenic drug (ethylmethanesulfonate), mutant clones of Ob17 preadipose cells resistant to the cytotoxic action of ouabain have been isolated. Their ability to grow in serum-supplemented medium containing greater than or equal to 3 mM ouabain is consistent with the decreased ouabain sensitivity of K+ transport observed in one of these clones (Ob17-OR11). Ouabain-resistant mutants retain their ability to convert into adipose-like cells in vitro with a frequency similar to that of original Ob17 cells. Long-term maintenance of Ob17-OR11 cells in ouabain-enriched medium leads to a higher frequency of adipose conversion, which is linked to a rather specific and limited growth-promoting effect of the drug. Undifferentiated Ob17-OR11 cells, when injected subcutaneously into athymic mouse, give rise in vivo to a fat pad containing ouabain-resistant mature adipocytes. Therefore, Ob17-OR11 clone should be a useful tool to distinguish in vivo between intrinsic and extrinsic factors involved in the differentiation of adipose precursor cells.
Gaillard
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Poli
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Négrel
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D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D003066:Coenzyme A Ligases / Q000378:metabolism; D003470:Culture Media; D004351:Drug Resistance; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009154:Mutation; D010042:Ouabain / Q000494:pharmacology*; D010641:Phenotype; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D003470:Culture Media; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D010042:Ouabain; D005993:Glycerolphosphate Dehydrogenase; D008071:Lipoprotein Lipase; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase; D000254:Sodium-Potassium-Exchanging ATPase