Analysis of the HSV-2 early AG-4 antigen.
Genital herpes simplex virus type 2 (HSV-2) infections can be distinguished from present or past HSV-1 infections by an AG-4 antigen complement fixation assay. The assay which utilizes a 4 hour HSV-2 infected cell extract prepared at a multiplicity of infection (MOI) of 1.0 PFU/cell, appears to consist of several viral proteins. Studies using monoclonal antibodies, polyclonal rabbit hyperimmune serum, HSV-1 X HSV-2 intertypic recombinant viruses and polyacrylamide gel electrophoresis suggest that ICP 8 may be one of the major antigens involved in the complement fixing reaction. It is probable that the success of the assay is not due to a true type specificity but rather a threshold phenomenon in which HSV-2 extracts contain more early viral antigens (including ICP 8) and sera from HSV-2 patients contain more complement fixing antibody to these antigens.
Evans
L A
LA
Sheppard
M
M
May
J T
JT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D003168:Complement Fixation Tests; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D018139:Simplexvirus / Q000276:immunology*; D014764:Viral Proteins / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014764:Viral Proteins