title: Sodium balance effects on renin, angiotensinogen, and atrial natriuretic polypeptide mRNA levels.
abstract: The expressions of the renin, angiotensinogen, and atrial natriuretic polypeptide (ANP) genes were studied in rats with varying sodium intakes. mRNAs for angiotensinogen and renin were measured by a sensitive radiodensitometric hybridization assay. ANP mRNA was determined by a dot-blot hybridization assay. In the high-sodium state, plasma renin concentration (PRC), renal renin concentration (RRC), and renal renin mRNA decreased by 88, 90, and 75%, respectively. In the low-sodium state, PRC, RRC, and renin mRNA increased 17-fold, 2.5-fold, and 4.5-fold, respectively. With captopril treatment during sodium depletion, PRC and renin mRNA increased 144-fold and 17.1-fold, respectively, and RRC increased 4.2-fold. The angiotensinogen mRNA level in the liver and kidney decreased in the high-sodium and increased in the low-sodium state to similar extents. Captopril treatment decreased angiotensinogen mRNA in both organs by about half. Plasma ANP and ANP mRNA decreased in the low- compared with the high-sodium state, whereas atrial ANP content was unaltered in both low- and high-sodium states. These results demonstrate that sodium intake affects the expression of the renin and angiotensinogen genes and slightly alters the expression of ANP gene.
authors.affiliation: Department of Pharmacology, Kagawa Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iwao
authors.forename: K
authors.initials: K
authors.lastname: Fukui
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Nakayama
authors.forename: H
authors.initials: H
authors.lastname: Ohkubo
authors.forename: S
authors.initials: S
authors.lastname: Nakanishi
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
mesh_terms: D000450:Aldosterone / Q000097:blood; D000804:Angiotensin II / Q000097:blood; D000808:Angiotensinogen / Q000235:genetics*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D007668:Kidney / Q000201:enzymology; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012083:Renin / Q000097:blood / Q000235:genetics*; D012084:Renin-Angiotensin System / Q000187:drug effects; D012982:Sodium, Dietary / Q000494:pharmacology*
chemical_list: D012333:RNA, Messenger; D012982:Sodium, Dietary; D000808:Angiotensinogen; D000804:Angiotensin II; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D012083:Renin