[Genetic transformation of somatic cells. VI. Transfer of the trait of the rate of loss of transformant phenotype by means of DNA from cells containing the thymidine kinase gene of the herpes simplex virus].
Using dot-hybridization with thymidine kinase gene (tk gene) of Herpes simplex virus type 1 (HSV 1) of DNA preparations obtained from isolated metaphase chromosomes and lysate fractions of metaphase cells, which presumably contain smaller particles compared to metaphase chromosomes, it has been shown that the tk gene of HSV 1 is localized in chromosomes of cells of transformant clones unstable in TK+-phenotype. The DNA isolated from the metaphase chromosomes from cells of transformant clones is 1.5- or 2-fold more efficient in transforming TK-Chinese hamster cells than is the total high molecular weight DNA from the same cells. Upon transformation of TK- cells by the high molecular weight DNA from the tk gene of HSV 1-containing clones, varying in the rate of the loss of TK+-phenotypes, the character "rate of the loss of transformant phenotype" is transferred together with the tk gene of HSV 1 in 22% of cases. Cells of rerevertant clones, produced from TK- subclones of transformant clones, display the rate of the loss of transformant phenotype characteristic of cells of parental TK+-clones. A comparison of the results allows a conclusion that DNA sequences, determining the character "rate of the loss of transformant phenotype", are linked tightly with the transforming DNA proper containing the tk gene of HSV 1, but are not localized inside such a DNA.
Glebov
O K
OK
Abramian
D S
DS
D000818:Animals; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000166:cytology / Q000201:enzymology; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008677:Metaphase; D010641:Phenotype; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014170:Transformation, Genetic
D004279:DNA, Viral; D004247:DNA; D013937:Thymidine Kinase
Geneticheskaia transformatsiia somaticheskikh kletok. VI. Peredacha priznaka "skorost' poteri transformantnogo fenotipa" s pomoshch'iu DNK iz kletok, soderzhashchikh gen timidinkinazy virusa prostogo gerpesa.