Methodology in preserving field-collected flies for bluetongue virus assay.
Preliminary tests were conducted to evaluate the use of phosphate buffered saline (PBS) as a collecting medium for the preservation of flies for virus assay from our bluetongue (BT) study areas in Colorado, USA. These tests were made possible by the use of a colony of Culicoides variipennis (Coquillett) that is highly susceptible to infection with BT virus (BTV).
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D000818:Animals; D001820:Bluetongue virus / Q000302:isolation & purification*; D002519:Ceratopogonidae / Q000382:microbiology*; D007303:Insect Vectors / Q000382:microbiology*; D012087:Reoviridae / Q000302:isolation & purification*; D012107:Research Design