[The viroimmunotest for mass determinations of nanogram amounts of antigens].
Simple highly sensitive screening variant of viral immunoassay for detection of nanogram quantity of antigen is proposed. The antigen linked with antibodies adsorbed on polystyrene 96-well plates was revealed by conjugate of Fab' fragments of antibodies with phage phi X174. The concentration of the antigen could be detected qualitatively and quantitatively (from 0.3 ng/ml to 1000 ng/ml).
Skvortsov
V T
VT
Suslov
A P
AP
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D010584:Bacteriophage phi X 174 / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008403:Mass Screening / Q000379:methods*; D011817:Rabbits; D051381:Rats; D010948:Viral Plaque Assay
D000956:Antigens, Viral; D007140:Immunoglobulin Fab Fragments
Viroimmunotest dlia massovykh opredeleniĭ nanogrammovykh kolichestv antigena.