title: Allogeneic lymphocyte stimulation by human spleen. Identification of cells that are very effective for presenting class II HLA antigens.
abstract: Human spleen cells were fractionated by percoll density gradient centrifugation and by sorting in the FACS with mixtures of fluorescent antibodies against T cells, B cells, monocytes, and Sig-bearing cells. Cells responsible for powerful MLR stimulation were class II HLA antigen-positive and were concentrated in preparations depleted of all the markers listed above. These cells represented 1-2% of the initial spleen cells. They were remarkably more active than other HLA class II antigen-positive cells. The procedure described allows rapid enrichment for the responsible cells. It should be useful for further characterization of these cells and for performing studies on their function.
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: C A
authors.initials: CA
authors.lastname: Pettaway
authors.forename: E J
authors.initials: EJ
authors.lastname: Ball
authors.forename: P
authors.initials: P
authors.lastname: Stastny
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D002448:Cell Adhesion; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D006684:HLA-DR Antigens; D011947:Receptors, Antigen, B-Cell