Percoll and Ficoll self-generated density gradients by low-speed osmocentrifugation.
Percoll and Ficoll self-generated density gradients can be obtained by low-speed centrifugation of their solutions within dialysis cells. Useful Percoll density gradients can be obtained after 10-30 min centrifugation at 220-2010g, within dialysis cells. Ficoll density gradients, which are more difficult to self-generate, can be obtained by the same technique. Red cell band formation in a Percoll density gradient can be done in a single step by using dialysis cells as the centrifugation solution container.
Nunes
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Galembeck
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D002499:Centrifugation, Density Gradient / Q000379:methods*; D055598:Chemical Phenomena; D002621:Chemistry; D003956:Dialysis; D005362:Ficoll; D009994:Osmolar Concentration; D011134:Polysaccharides; D011205:Povidone; D012822:Silicon Dioxide
D011134:Polysaccharides; D005362:Ficoll; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone