[Mechanism of action of interferon-alpha 2 in hairy cells and lymphoblastoid cell lines: the significance of type I interferon receptors and RNA synthesis].
Lymphoblastoid cells were subjected to Western Blot analysis for detection of Interferon-alpha-binding proteins. JOK-1 cells--a human hairy cell leukemia line--revealed three proteins with apparent molecular weights of 120, 100 and 32 kD, respectively. Down-regulation of the receptor was observed. A differential binding pattern of two proteins (100 and 85 kD) was observed in T-CLL, whereas no signal detection was achieved in B-CLL. Mononuclear cells from 6 patients with hairy cell leukemia and 6 patients with CLL were found to differ significantly in terms of nucleic acid precursor incorporation.
Schwabe
M
M
1. Medizinische Universitätsklinik, Wien, Osterreich.
Wagner
L
L
Prischl
F
F
Pinter
G
G
Schwarzmeier
J D
JD
D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D007943:Leukemia, Hairy Cell / Q000276:immunology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D011971:Receptors, Immunologic / Q000187:drug effects*; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D007370:Interferon Type I; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D012334:RNA, Neoplasm; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins
Zum Wirkungsmechanismus von Interferon-alpha 2 bei Haarzellen und lymphoblastoiden Zellinien: Die Bedeutung von Typ-I-Interferon-Rezeptoren und RNS-Synthese.