beta-Adrenergic activation of glycosyltransferases in the dolichylmonophosphate-linked pathway of protein N-glycosylation.
beta-Adrenoreceptor stimulation of rat parotid acinar cells increases the activity of several microsomal membrane associated, dolichylmonophosphate (Dol-P) linked glycosyltransferases. The activities of Man-P-Dol synthase and Glc-P-Dol synthase are increased by approximately 50%, and the activity of N-acetylglucosaminyl 1-phosphate transferase plus N-acetylglucosaminyl transferase increased by approximately 60%, after agonist treatment. Increases in enzyme activity are (i) independent of endogenous Dol-P levels and (ii) observed under conditions in which the specific activities of donor sugar nucleotides are kept constant. Activation of these enzymes is specific since comparable levels of NADPH-cytochrome c reductase are found in control and agonist-treated membranes. The data thus provide the initial demonstration of neurotransmitter modulation of enzymes in the dolichol-linked pathway of protein N-glycosylation.
Banerjee
D K
DK
Kousvelari
E E
EE
Baum
B J
BJ
D000818:Animals; D004288:Dolichol Phosphates / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D010306:Parotid Gland / Q000201:enzymology; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology
D004288:Dolichol Phosphates; D011106:Polyisoprenyl Phosphates; D011943:Receptors, Adrenergic, beta; D014415:Tunicamycin; C028306:dolichol monophosphate; D006602:Hexosyltransferases; D007545:Isoproterenol