Physical characterization of the genome of the Myxococcus xanthus bacteriophage MX-8.
We have constructed a restriction map for the genome of bacteriophage MX-8 from Myxococcus xanthus using the enzymes PvuII, MboI, and EcoRI. The phage genome size, as determined by restriction analysis, is 51.7 +/- 0.6 Kb. Double digestions, redigestions of isolated fragments, and crossed-contact hybridization of partial digestion products show that the restriction map is circular. Restriction analysis and Southern hybridization show that the phage DNA molecules are packaged sequentially from a concatemer starting from a specific site which we have mapped. The DNA molecules have an average terminal redundancy of approximately 8% and are circularly permuted over at least 40% of the genome.
Stellwag
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Fink
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Zissler
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D001435:Bacteriophages / Q000235:genetics* / Q000648:ultrastructure; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D008970:Molecular Weight; D009231:Myxococcales / Q000235:genetics*; D009690:Nucleic Acid Conformation
D004270:DNA, Circular; D004279:DNA, Viral; D004262:DNA Restriction Enzymes