title: Fluorescence line narrowing-nonphotochemical hole burning spectrometry: femtomole detection and high selectivity for intact DNA-PAH adducts.
abstract: A new fluorescence line narrowing (FLN) apparatus is described and evaluated through experiments on intact DNA-PAH (polycyclic aromatic hydrocarbon) and globin-PAH adducts, as well as polar PAH metabolites. A detection limit of approximately 3 modified bases in 10(8) for a DNA adduct formed with a diol-epoxide of benzo[a]pyrene (BPDE-DNA) is reported for 20 micrograms of DNA at a spectral resolution of approximately 8 cm-1. The methodology employed avoids or minimizes spectral degradation and loss of sensitivity due to photooxidation and nonphotochemical hole burning (NPHB). A new double selection technique that employs both FLN and NPHB is described and found to lead to a significant improvement in selectivity over that obtained with conventional FLN.
authors.affiliation: Ames Laboratory--USDOE and Department of Chemistry, Iowa State University, Ames 50011.
authors.forename: R
authors.initials: R
authors.lastname: Jankowiak
authors.forename: R S
authors.initials: RS
authors.lastname: Cooper
authors.forename: D
authors.initials: D
authors.lastname: Zamzow
authors.forename: G J
authors.initials: GJ
authors.lastname: Small
authors.forename: G
authors.initials: G
authors.lastname: Doskocil
authors.forename: A M
authors.initials: AM
authors.lastname: Jeffrey
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis*; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D018736:DNA Adducts; D008832:Microchemistry; D011083:Polycyclic Compounds / Q000032:analysis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D018736:DNA Adducts; D011083:Polycyclic Compounds; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA