title: Splenic leukocytes from chickens injected with Salmonella pullorum antigen stimulate production of corticosteroids by isolated adrenal cells.
abstract: Corticosteroid levels in the blood serum of White Rock pullets were significantly increased within 1 hr after an iv injection of heat-killed Salmonella pullorum antigen (SP-Ag), and reached levels 7 to 10 times that of PBS-injected controls within 2 hr after SP-Ag injection. Incubations with isolated adrenal cells indicated that serum from SP-Ag-injected birds had the ability to stimulate the synthesis or release of corticosteroids twice that of serum PBS-injected birds. Stripping the serum from SP-Ag-injected birds with activated charcoal and precipitated silica (Quso G-32) removed the corticosteroids and the adrenal-stimulating ability. A 2-hr incubation of isolated adrenal cells with leukocytes from spleens removed from chickens 1 hr after injection with SP-Ag, using stripped serum as the medium, stimulated a two- to fivefold increase in corticosteroid as compared to splenic leukocytes from PBS-injected chickens incubated in the same medium. The results indicate that an "ACTH-like" substance was produced by the S. pullorum antigen-stimulated splenic leukocytes.
authors.forename: H S
authors.initials: HS
authors.lastname: Siegel
authors.forename: N R
authors.initials: NR
authors.lastname: Gould
authors.forename: J W
authors.initials: JW
authors.lastname: Latimer
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology* / Q000378:metabolism; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis* / Q000097:blood; D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002645:Chickens; D005260:Female; D007962:Leukocytes / Q000276:immunology / Q000502:physiology*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D051381:Rats; D012475:Salmonella / Q000276:immunology*; D013154:Spleen / Q000166:cytology
chemical_list: D000305:Adrenal Cortex Hormones; D000942:Antigens, Bacterial; D008077:Lipoproteins, LDL; D000324:Adrenocorticotropic Hormone