A comparison of the effects of cytotoxic cerebrospinal fluid on cell cultures with other cytopathogenic agents.
Protein synthesis, antigen synthesis, and cell membrane permeability were analyzed after inoculating human diploid fibroblasts with control or cytotoxic CSF, herpes simplex virus type 1 (HSV 1), poliovirus 3, or Clostridium difficile toxin. Whereas protein synthesis and membrane permeability were affected by the viruses, and virus antigens detectable by pooled human serum were synthesized, the bacterial toxin and cytotoxic CSF did not induce any new proteins or antigens, although the cytotoxic CSF reduced cellular protein synthesis levels and caused an increase in the permeability of the cell membranes. The effect of the cytotoxic CSF in cell culture resembles that of a toxin rather than a replicating virus.
Taylor
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Carter
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Crow
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D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000494:pharmacology; D002478:Cells, Cultured; D002555:Cerebrospinal Fluid / Q000382:microbiology / Q000502:physiology*; D003013:Clostridium / Q000502:physiology; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D017955:Poliovirus / Q000276:immunology / Q000502:physiology; D014176:Protein Biosynthesis; D018139:Simplexvirus / Q000276:immunology / Q000502:physiology; D013936:Thymidine / Q000378:metabolism
D000956:Antigens, Viral; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C031815:toxB protein, Clostridium difficile; D013936:Thymidine