The distribution of 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNP) in the CNS of normal (+/+) and shiverer (Shi/Shi) mice.
Immunohistochemical techniques were utilized to localize the putative myelin enzyme 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNP) in the central nervous system (CNS) of normal (+/+) and Shiverer (Shi/Shi) mice (Mus musculus). CNP appeared to be only associated with myelinated nerve fibers in the CNS (corpus callosum, subcortical white matter, caudate nucleus, cerebellum and medulla oblongata) of +/+ mice. However, little or no immunostaining was observed in the same regions of the CNS of Shi/Shi mice, although these mice have normal levels of a CNS CNP activity. Unexpectedly, oligodendrocytes (cell periphery) were not stained for CNP in either +/+ or Shi/Shi mice, although erythrocytes were immunostained.
Sheedlo
H J
HJ
Sprinkle
T J
TJ
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002421:Caudate Nucleus / Q000201:enzymology; D002531:Cerebellum / Q000201:enzymology; D003337:Corpus Callosum / Q000201:enzymology; D007124:Immunoenzyme Techniques; D008297:Male; D008526:Medulla Oblongata / Q000201:enzymology; D051379:Mice; D008818:Mice, Neurologic Mutants; D009413:Nerve Fibers, Myelinated / Q000201:enzymology; D009836:Oligodendroglia / Q000201:enzymology
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; C039809:2',3'-cyclic nucleotide 2'-phosphodiesterase