Cloning and characterization of the yeast nuclear gene for subunit 5 of cytochrome oxidase.
The nuclear gene COX5 coding for subunit 5 of cytochrome oxidase has been cloned by transformation of the cox5-1 mutant aE4-238/AL1 with a library of yeast genomic DNA. The recombinant plasmid pG46/ST2 bearing a nuclear DNA insert of 1.17 kilobase pairs restores the ability of cox5 mutants to respire and to synthesize a wild type subunit 5. The COX5 gene has been sequenced and determined to code for a 153-amino acid long protein with a molecular weight of 17,121. The amino-terminal 20 residues comprise the signal peptide. The sequence starting from residue 21 matches the partial sequence reported for the mature subunit 5. The sequence of the subunit 5 gene indicates that the mature protein has a molecular weight of 14,858 which agrees with previous size estimates based on electrophoretic migration. The primary sequence and polarity profile of yeast subunit 5 establishes that it is homologous to subunit 4 of bovine cytochrome oxidase.
Koerner
T J
TJ
Hill
J
J
Tzagoloff
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D003576:Electron Transport Complex IV / Q000235:genetics*; D005796:Genes; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology; D009492:Neurospora crassa; D015003:Yeasts / Q000201:enzymology / Q000235:genetics
D046911:Macromolecular Substances; D003576:Electron Transport Complex IV