title: Dolichol kinase in Ascaris suum and Onchocerca volvulus.
abstract: Dolichol kinase was demonstrated in the microsomal fraction of Ascaris suum and Onchocerca volvulus. The enzyme from nematodes exhibited specificity for CTP as phosphoryl donor and was found to be inhibited by the reaction product CDP. Enzyme activity was optimal at pH 7.4, in the temperature range between 30 degrees and 37 degrees C, and in the presence of 0.5% Triton X-100. In addition, the enzyme was found to depend on divalent cations for activity; Mg2+ being more effective than Mn2+ and Ca2+. The dolichol kinase from both nematodes was shown to be independent of Ca2+-calmodulin for activity. The apparent Km values for dolichol were determined to be 7.5 and 9.0 micrograms ml-1 for the enzyme from A. suum and O. volvulus, respectively. Those for CTP were estimated to be 0.85 and 0.75 mM, respectively.
authors.forename: R D
authors.initials: RD
authors.lastname: Walter
authors.forename: E
authors.initials: E
authors.lastname: Ossikovski
authors.forename: E J
authors.initials: EJ
authors.lastname: Albiez
mesh_terms: D000818:Animals; D001200:Ascaris / Q000201:enzymology*; D002413:Cations, Divalent / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009854:Onchocerca / Q000201:enzymology*; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011092:Polyethylene Glycols / Q000494:pharmacology; D013347:Subcellular Fractions / Q000378:metabolism; D013696:Temperature; D014268:Trifluoperazine / Q000494:pharmacology
chemical_list: D002413:Cations, Divalent; D014268:Trifluoperazine; D011092:Polyethylene Glycols; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020087:dolichol kinase