Intracellular transport of herpes simplex virus gD occurs more rapidly in uninfected cells than in infected cells.
A mouse L cell line which expresses the herpex simplex virus type 1 immediate-early polypeptides ICP4 and ICP47 was cotransfected with a cloned copy of the BglII L fragment of herpes simplex virus type 2, which includes the gene for gD, and the plasmid pSV2neo, which contains the aminoglycosyl 3'-phosphotransferase (agpt) gene conferring resistance to the antibiotic G418. A G418-resistant transformed cell line was isolated which expressed herpes simplex virus type 2 gD at higher levels than were found in infected cells. The intracellular transport and processing of gD was compared in transformed and infected cells. In the transformed Z4/6 cells gD was rapidly processed and transported to the cell surface; in contrast, the processing and cell surface appearance of gD in infected parental Z4 cells occurred at a much slower rate, and gD accumulated in nuclear membrane to a greater extent. Thus, the movement of HSV-2 gD to the cell surface in infected cells is retarded as viral glycoproteins accumulate in the nuclear envelope, probably because they interact with other viral structural components.
Johnson
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DC
Smiley
J R
JR
D000818:Animals; D001692:Biological Transport; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006561:Herpes Simplex / Q000378:metabolism*; D051379:Mice; D014176:Protein Biosynthesis; D018139:Simplexvirus / Q000235:genetics; D014764:Viral Proteins / Q000378:metabolism*
D006023:Glycoproteins; D014764:Viral Proteins