title: Glycosylation and cross-linkage of cardiac myosin in diabetic subjects: a post-mortem study.
abstract: We have investigated the possibility that post-translational modification of myosin by protein glycosylation and cross-linking occurs in cardiac myosin. Left ventricular muscle was obtained at post-mortem from 6 diabetic and 7 non-diabetic subjects. Myosin was extracted from muscle and purified using Sephadex chromatography followed by protein concentration. Glycosylation was estimated using boronate affinity chromatography with the myosin dissolved in a pyrophosphate buffer, the glycosylated myosin being displaced with sorbitol. Cross-linkage was assessed by fluorescence at 440 nm upon excitation at 370 nm. Diabetic subjects had significantly higher levels (p less than 0.02) of glycosylated myosin (median 6.0% (range 3.8-6.6%] than non-diabetic subjects (median 2.4% (range 0.3-4.2%] but there was no difference in the degree of cross-linkage as assessed by fluorescence (diabetic median 9.8 (range 6.5-17.0) arbitrary units; non-diabetic median 9.7 (range 6.0-11.4) arbitrary units). Glycosylation of left ventricular myosin may be of relevance to the excess risk of congestive cardiac failure in diabetic patients.
authors.affiliation: Department of Medicine, University College and Middlesex School of Medicine, London, UK.
authors.forename: J S
authors.initials: JS
authors.lastname: Yudkin
authors.forename: M B
authors.initials: MB
authors.lastname: Cooper
authors.forename: B J
authors.initials: BJ
authors.lastname: Gould
authors.forename: J
authors.initials: J
authors.lastname: Oughton
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D003920:Diabetes Mellitus / Q000378:metabolism*; D005260:Female; D006031:Glycosylation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D012016:Reference Values
chemical_list: D009218:Myosins