Equine herpesvirus type 2: cell-virus relationship during persistent cell-associated viremia.
Some aspects of the biology of equine herpesvirus type 2 (EHV-2) were investigated by examination of the persistent cell-associated viremia stage of the infection. The EHV-2 infection of leukocytes was latent, because free virus was not retrieved without first cultivating harvested leukocytes in vitro. A virus infective center (IC) assay was developed to enumerate latently infected cells in the leukocyte population. This assay proved to be simple and reproducible and revealed a linear relationship between IC plaques formed and the number of cells inoculated, except where large numbers of cells (greater than 4 X 10(6)) were inoculated per 10 cm2 dish. This reduction at high cell densities of IC/10(6) cells inoculated was dependent on cells obtained from an EHV-2-infected horse. There was considerable variation in the numbers of IC/10(6) leukocytes harvested from different horses, but little variation in the harvests from the same horse at different times. There seemed to be a direct relationship between serum-neutralization titers and IC numbers. Transfer of viable infected leukocytes to 2 fetuses failed to establish EHV-2 infection. Infection of equine fetal kidney cells with EHV-2 virus failed to produce detectable Fc receptors on the cell surface.
Gleeson
L J
LJ
Coggins
L
L
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D006564:Herpesviridae / Q000254:growth & development* / Q000276:immunology; D006566:Herpesviridae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D006734:Horse Diseases / Q000276:immunology / Q000382:microbiology*; D006736:Horses; D007962:Leukocytes / Q000382:microbiology*; D009500:Neutralization Tests; D010948:Viral Plaque Assay / Q000662:veterinary; D014766:Viremia / Q000382:microbiology / Q000662:veterinary*
D000914:Antibodies, Viral