Identification of the antigen-binding molecule on helper T cell lines specific for L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT).
GAT-specific helper T cell lines were shown to bind GAT, coupled to sepharose or sheep erythrocytes. The binding of T cell lines to GAT was specific, since forming of rosettes with GAT-SRBC was inhibited by pretreatment of T cells with free GAT or anti-idiotypic antibodies. Surface iodination of cells and purification of cell lysates on GAT-sepharose, revealed the presence of a 66 KD molecule and a smaller, 33 KD fragment. The proteins could be eluted specifically from GAT-sepharose with a free GAT, or nonspecifically with SDS. The 66 KD molecule could not be reduced by 5% 2-mercaptoethanol. In addition, it was found that the GAT-binding molecule did not bind to the antibodies against the heterodimer (T-cell receptor). The results suggest that the antigen receptor for GAT on helper T cells and inducer suppressor T cells may be related. The GAT-binding molecule may be loosely associated with the heterodimer.
Zimecki
M
M
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Kapp
J A
JA
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D011108:Polymers; D011487:Protein Conformation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D012397:Rosette Formation; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D007130:Immunoglobulin Idiotypes; D010455:Peptides; D011108:Polymers; D011948:Receptors, Antigen, T-Cell; C022204:anti-GAT antibodies, idiotypic; C020749:GAT