Photodynamic effects of hematoporphyrin-derivative on enzyme activities of murine L929 fibroblasts.
Photodynamic treatment of murine L929 fibroblasts with hematoporphyrin-derivative resulted in the inactivation of cytosolic, mitochondrial and lysosomal enzymes and in a decrease in cellular adenosine triphosphate and reduced glutathione concentrations. Comparison of these results with those of previous studies revealed that transmembrane transport systems and DNA repair enzymes are inactivated after much shorter illumination periods than are intracellular enzymes. Although the pattern of photodynamic damage altered by varying the protocol of preincubation with hematoporphyrin-derivative and washing, it appeared that under all experimental conditions the plasma membrane was much more sensitive to photodynamic damage than were the intracellular enzymes. Lysosomal membrane disruption with subsequent detrimental release of lysosomal enzymes has been implicated previously in certain forms of porphyrin-induced photodynamic cell destruction. Cytochemical studies on enzyme localization virtually exclude such a mechanism in hematoporphyrin-derivative-induced cell inactivation in L929 fibroblasts.
Boegheim
J P
JP
Sylvius Laboratories, Department of Medical Biochemistry, Leiden, The Netherlands.
Scholte
H
H
Dubbelman
T M
TM
Beems
E
E
Raap
A K
AK
van Steveninck
J
J
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004798:Enzymes / Q000378:metabolism; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000494:pharmacology*; D007700:Kinetics; D007739:L Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
D004798:Enzymes; D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative