title: Suppression of contractile force in muscle fibers by antibody to myosin subfragment 2.
abstract: Polyclonal antibody directed against the subfragment-2 region of myosin was purified by affinity chromatography. Skinned muscle fibers that had been preincubated with antibody were able to sustain only 7% of the active isometric force generated by control fibers. The effect of antibody on force production could not be accounted for by inhibition of ATP turnover.
authors.affiliation: Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
authors.forename: S
authors.initials: S
authors.lastname: Lovell
authors.forename: T
authors.initials: T
authors.lastname: Karr
authors.forename: W F
authors.initials: WF
authors.lastname: Harrington
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D002645:Chickens; D005998:Glycine; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D009119:Muscle Contraction; D015879:Myosin Subfragments; D009218:Myosins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D051381:Rats
chemical_list: D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D015879:Myosin Subfragments; D010446:Peptide Fragments; D000251:Adenosine Triphosphatases; D009218:Myosins; D005998:Glycine