[Development of new plate tests for the detection of microbial hydrolysis of esters and oxidation of 2-hydroxycarboxylic acids].
The application of the thin-agar-layer coated filter culture technique (Z. Naturforsch. 42c, 1082 (1987)) has been extended to the detection of ester hydrolysis and 2-hydroxyacid oxidation by microbial colonies. The former was performed by spraying with bromocresol purple and the latter with a salicylhydrazide reagent. Under the optimized conditions, the hydrolysis activity of more than 20 mumol/h.g-wet cells and the oxidation activity of more than 40 mumol/h.g-wet cells were usually detected directly on the filter-plate cultures of bacteria, yeasts and molds.
Yamazaki
Y
Y
Institut für Enzymtechnologie der Universität Düsseldorf in der Kernforschungsanlage Jülich, Bundesrepublik Deutschland.
Kula
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MR
D000421:Alcaligenes / Q000378:metabolism; D001236:Aspergillus oryzae / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D004952:Esters; D005658:Fungi / Q000378:metabolism*; D006868:Hydrolysis; D006880:Hydroxy Acids / Q000378:metabolism*; D007202:Indicators and Reagents; D009090:Mucorales / Q000378:metabolism; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D004952:Esters; D006880:Hydroxy Acids; D007202:Indicators and Reagents
Entwicklung neuer Plattentests zum Nachweis mikrobieller Hydrolysen von Estern und Oxidationen von 2-Hydroxycarbonsäuren.