title: Effects of N-terminal peptide of salmon proopiocortin on interrenal function of the rainbow trout.
abstract: Effects of N-terminal peptide of salmon proopiocortin (salmon NPP-I) on cortisol secretion was examined in vitro using diced interrenal tissue from the rainbow trout, Salmo gairdneri. ACTH(1-24) at concentrations of 1 to 50 nM stimulated cortisol secretion in dose-dependent manner, whereas salmon NPP-I had no effect over a range of 50 pM to 500 nM. Cortisol secretion in response to various doses of ACTH(1-24) was modified slightly when 1 to 100 nM of salmon NPP-I was added to the incubation medium together with ACTH. An augmentation of in vitro secretion of cortisol in response to ACTH(1-24) was observed when the interrenal was removed from the trout pretreated with one IU of porcine ACTH but not with 10 micrograms of salmon NPP-I. A slight but significant potentiating effect of salmon NPP-I (10 or 100 nM) on the ACTH-induced cortisol secretion was observed when the trout was sensitized to ACTH by porcine ACTH pretreatment. Furthermore, six daily injections of salmon NPP-I into the trout induced hyperplasia of interrenal tissue. These findings suggest that NPP-I, together with ACTH, may be involved in controlling interrenal function in the trout. Such activities could be due to conservative region in the N-terminal portion of NPP.
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.forename: J
authors.initials: J
authors.lastname: Kubota
authors.forename: H
authors.initials: H
authors.lastname: Kawauchi
authors.forename: T
authors.initials: T
authors.lastname: Hirano
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000502:physiology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000805:Angiotensin III / Q000494:pharmacology; D000818:Animals; D003366:Cosyntropin / Q000494:pharmacology; D004357:Drug Synergism; D010446:Peptide Fragments / Q000494:pharmacology; D011333:Pro-Opiomelanocortin / Q000494:pharmacology*; D012474:Salmon; D012487:Salmonidae / Q000502:physiology*; D014337:Trout / Q000502:physiology*; D014668:Vasotocin / Q000494:pharmacology
chemical_list: D010446:Peptide Fragments; D000804:Angiotensin II; D000805:Angiotensin III; D003366:Cosyntropin; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D014668:Vasotocin