title: Comparison of media with and without 'Panmede' for the isolation of Streptobacillus moniliformis from blood cultures and observations on the inhibitory effect of sodium polyanethol sulphonate.
abstract: Fastidious anaerobe broth and brain-heart infusion cysteine broth supplemented with 'Panmede' (a papain digest of ox liver) 2.5% supported the recovery of five Streptobacillus moniliformis strains from simulated blood cultures. Other media tested in parallel--brain heart infusion cysteine broth without 'Panmede' and Brewer's thioglycollate broth--were unreliable. Sodium polyanethol sulphonate (Liquoid) 0.05%, inhibited five isolates of S. moniliformis, including isolates from patients with Haverhill Fever. Occasionally, Liquoid 0.025% was also inhibitory and a heavy inoculum of one strain, NCTC11194, was completely inhibited by Liquoid 0.012% in simulated nutrient-broth blood cultures. These results suggest that the choice of media included in each blood-culture set is critical for the optimal isolation of S. moniliformis. Brain-heart infusion cysteine broth supplemented with 'Panmede', or commercially available fastidious anaerobe broth, without Liquoid, is recommended.
authors.forename: D C
authors.initials: DC
authors.lastname: Shanson
authors.forename: J
authors.initials: J
authors.lastname: Pratt
authors.forename: P
authors.initials: P
authors.lastname: Greene
mesh_terms: D000693:Anaerobiosis; D001557:Benzenesulfonates / Q000494:pharmacology*; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D006801:Humans; D008099:Liver; D010206:Papain; D011074:Polyanetholesulfonate / Q000494:pharmacology*; D011906:Rat-Bite Fever / Q000382:microbiology*; D018805:Sepsis / Q000382:microbiology; D013288:Streptobacillus / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D001557:Benzenesulfonates; D003470:Culture Media; D011074:Polyanetholesulfonate; D010206:Papain