Observations on cell kinetics and viability of a human melanoma cell line exposed to dicarboxylic acids in tissue culture.
Cultures of human melanoma cell line B0008 were exposed to the disodium salts of azelaic acid (C9 2Na), adipic acid (C6 2Na) and dodecanediaic acid (C12 2Na) at 10(-2) M and 5 x 10(-2) M for 24 hrs. None of the diacid salts had a significant effect on growth rate or viability of the cells, at 10(-2) M for 24 hrs nor had C6 2Na any effect at 5 x 10(-2) M. At 5 x 10(-2) M for 24 hrs, both C9 2Na, and C12 2Na had a significant effect in reducing both growth and viability. These effects were accompanied by morphological evidence of cell death, and swelling of mitochondria and accumulation of lipid droplets within cytoplasm of still viable cells.
Breathnach
A S
AS
Department of Anatomy, St. Mary's Hospital Medical School, London, UK.
Robins
E J
EJ
Bhasin
Y
Y
Ethridge
L
L
Nazzaro-Porro
M
M
Passi
S
S
Picardo
M
M
D000272:Adipates / Q000494:pharmacology*; D002452:Cell Count / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003998:Dicarboxylic Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008545:Melanoma / Q000648:ultrastructure; D008928:Mitochondria / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000648:ultrastructure
D000272:Adipates; D003998:Dicarboxylic Acids; C029900:adipic acid; C036836:dodecanedioic acid; C010038:azelaic acid