title: Detection of deoxyribonucleoside-triphosphate imbalance death-induced DNA double strand breakage in FM3A cells by orthogonal-field-alternation gel electrophoresis (OFAGE).
abstract: The mechanism of intracellular deoxyribonucleoside-triphosphate (dNTP) imbalance death of mouse mammary tumor FM3A cells was studied. When the cells were exposed to 5-fluorodeoxyuridine, deoxyadenosine, or 2-chlorodeoxyadenosine, dNTP pool imbalance resulted. The imbalance was followed by DNA double strand breaks and subsequent cell death. The DNA double strand breaks have been directly examined by means of orthogonal-field-alternation gel electrophoresis (OFAGE). Fragmented DNA band appeared to be approximately 100-200 kb in size.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Wataya
authors.forename: T
authors.initials: T
authors.lastname: Hazawa
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Hirota
authors.forename: A
authors.initials: A
authors.lastname: Yoshioka-Hiramoto
mesh_terms: D000818:Animals; D002460:Cell Line; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects; D003854:Deoxyribonucleotides / Q000378:metabolism*; D005467:Floxuridine / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D051379:Mice
chemical_list: D004273:DNA, Neoplasm; D003854:Deoxyribonucleotides; D005467:Floxuridine