title: S13, a rapidly oncogenic replication-defective avian retrovirus.
abstract: The avian leukemia sarcoma virus S13 transforms chicken and Japanese quail embryo fibroblasts and chicken erythroid cells in tissue culture. S13-induced erythroid transformation requires culture conditions suitable for the growth of normal erythroid precursors (H. Beug and M. J. Hayman (1984), Cell 36, 963-972). S13-transformed erythroid colonies contain a high percentage of cells that differentiate in absence of erythropoietin. S13 is defective in pol and env functions but can code for a complete set of gag proteins. Nonproducer cell clones transformed by S13 release a noninfectious viral particle containing gag but no functional env or pol proteins. They also synthesize a transformation-specific protein of 155,000 molecular weight. This protein reacts with antibody to viral envelope glycoproteins and appears to represent onc as well as env sequences. The 155,000-molecular weight env-linked protein does not cross react immunologically with an antiserum against the v-erb A and v-erb B gene products.
authors.forename: H
authors.initials: H
authors.lastname: Beug
authors.forename: M J
authors.initials: MJ
authors.lastname: Hayman
authors.forename: T
authors.initials: T
authors.lastname: Graf
authors.forename: S H
authors.initials: SH
authors.lastname: Benedict
authors.forename: A M
authors.initials: AM
authors.lastname: Wallbank
authors.forename: P K
authors.initials: PK
authors.lastname: Vogt
mesh_terms: D001355:Alpharetrovirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000096:biosynthesis / Q000235:genetics; D001854:Bone Marrow Cells; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D003370:Coturnix; D003673:Defective Viruses / Q000502:physiology*; D004900:Erythroblasts; D004920:Erythropoiesis; D005347:Fibroblasts; D006378:Helper Viruses / Q000502:physiology; D009857:Oncogenes; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014779:Virus Replication
chemical_list: D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D014764:Viral Proteins