Nuclear testicular 1,25-dihydroxyvitamin D3 receptors in Sertoli cells and seminiferous tubules of adult rodents.
1,25(OH)2D3 receptors were studied in whole testes, Sertoli cells, seminiferous tubules, Leydig cells and spermatogonia of adult NMRI mice and SD rats. Specific reversible high affinity binding (KD 1.4 x 10(-10)M; Nmax 72 fmol/mg protein) by a 3.5 S macromolecule was demonstrated in whole testes, Sertoli cells and seminiferous tubules. With identical techniques, no receptors were found in Leydig cells despite previous reports of 1,25(OH)2D3 actions on Leydig cell function.
Merke
J
J
Hügel
U
U
Ritz
E
E
D000818:Animals; D002117:Calcitriol / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D004247:DNA / Q000031:analogs & derivatives / Q000378:metabolism; D007700:Kinetics; D007985:Leydig Cells / Q000032:analysis; D008297:Male; D051379:Mice; D051381:Rats; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000032:analysis*; D012671:Seminiferous Tubules / Q000032:analysis*; D012708:Sertoli Cells / Q000032:analysis*; D013093:Spermatogonia / Q000032:analysis; D013737:Testis / Q000032:analysis*
C033190:DNA-cellulose; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D002482:Cellulose; D004247:DNA; D002117:Calcitriol