Method for identification of intracellular free flavin species in the photosensitive fungus Neurospora crassa.
Establishing the relative intracellular proportions of flavins in Neurospora crassa (and in other organisms) in vivo may be hampered by degradation of flavins after homogenization of the cells. The system described here allows separation and identification of intracellular free and bound flavins under conditions restrictive for the FAD-degrading enzyme(s). A "protective buffer" containing 0.1 M citrate adjusted to pH 4.0 with K2HPO4, 5 mM ATP, and 0.5 mM EDTA prevents FAD from rapid enzymatic cleavage in crude cell lysates of the Neurospora crassa mutant "slime."
Scheideler
L
L
Institut für Chemische Pflanzenphysiologie, Universität Tübingen, Federal Republic of Germany.
Ninnemann
H
H
D002021:Buffers; D005182:Flavin-Adenine Dinucleotide / Q000302:isolation & purification; D005415:Flavins / Q000302:isolation & purification*; D009491:Neurospora / Q000032:analysis*; D009492:Neurospora crassa / Q000032:analysis*; D010777:Photochemistry
D002021:Buffers; D005415:Flavins; D005182:Flavin-Adenine Dinucleotide