[Cytometric analysis of the DNA content of the thymus, spleen and liver in C57BL/6 mice].
Flow cytometric analysis of cell DNA content in the mouse C 57 BL/6 shows better results with fine needle aspiration than with mechanical dissociation of the tissues. There is no difference fresh and deep frozen stored tissues. In the liver, it has been found two cell populations with different DNA content including diploid and tetraploid cell lines. In the spleen and the thymus, only diploid cells are encountered with different cell cycles belonging to the organ.
Vago
P
P
Laboratoire de Biologie cellulaire et d'Immunogénétique, Faculté de Médecine, Nîmes.
Abdoukhil
S
S
Odier
F
F
Mion
H
H
Bureau
J P
JP
D000818:Animals; D001707:Biopsy, Needle / Q000379:methods; D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry; D005615:Freezing; D008099:Liver / Q000032:analysis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000032:analysis*; D013950:Thymus Gland / Q000032:analysis*; D014021:Tissue Preservation
D004247:DNA
Analyse cytométrique du contenu en ADN du thymus, de la rate et du foie de la souris C 57 BL/6.