[Cell cycle analysis using the monoclonal antibody Ki-67].
We determined by flow cytometry the proportion of cells in cycle with the monoclonal antibody Ki-67 and also in S-phase after incorporation of bromodeoxyuridine (BrdUrd) with monoclonal antibody to BrdUrd. The monoclonal antibody Ki-67 was useful to detect a nuclear antigen present only in proliferating cells but not expressed in resting (Go) cells. Cell preparation to measure BrdUrd amount incorporated into cellular DNA was difficult but this anti-BrdUrd antibody was useful for measuring the rate of DNA synthesis and for the analysis of precious cell kinetics. These antibodies may provide useful information of cell kinetics.
Isobe
H
H
First Department of Internal Medicine, School of Medicine, Hokkaido University.
Shimizu
T
T
Haneda
H
H
Mizuno
S
S
Ishiguro
A
A
Araya
Y
Y
Harada
M
M
Miyamoto
H
H
Kawakami
Y
Y
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000276:immunology; D002453:Cell Cycle; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D019394:Ki-67 Antigen; D009687:Nuclear Proteins / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D004247:DNA; D001973:Bromodeoxyuridine