Mg2+-dependent adenosine triphosphatase: an enzyme marker for ovine T lymphocytes.
Sheep T lymphocytes showed a cell surface magnesium-dependent adenosine triphosphatase (Mg2+-ATPase) reaction, which is reported to be characteristic of human B lymphocytes. In cryostat sections of lymph nodes, spleen and thymus, Mg2+-ATPase positive regions closely matched those labelled by sheep pan T monoclonal antibodies (Moab). An Mg2+-ATPase reaction was also found in fibroblastic recticulum cells of T cell regions in lymph nodes. Double labelling of cells from peripheral blood and peripheral lymph for Mg2+-ATPase and the pan T marker showed that 78% of the lymphocytes were positive for both of these markers. In cell suspensions enriched for B lymphocytes the percentage of cells positively labelled was decreased to 37%. Samples of each cell population which were labelled with a pan T Moab and analysed by flow microfluorometry revealed T cell levels which were not significantly different from those obtained by histochemical or immunohistochemical techniques. Less than 1% of lymphocytes positive for heavy and light chains of immunoglobulin (Ig) G were labelled with Mg2+-ATPase. Veiled cells in lymph and monocytes showed a cytoplasmic Mg2+-ATPase reaction.
Alders
R G
RG
Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Landsverk
T
T
Shelton
J N
JN
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology; D015415:Biomarkers; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry; D007074:Immunoglobulin G / Q000378:metabolism; D011247:Pregnancy; D012756:Sheep; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007074:Immunoglobulin G; D017301:Ca(2+) Mg(2+)-ATPase