A novel inhibitor of mammalian collagenase.
N-[[[(5-chloro-2-benzothiazolyl)thiolphenyllacetyll-L-cysteine (WY-45,368) is a potent inhibitor of human skin fibroblast collagenase. Kinetic data show that the inhibition is competitive, with a Ki of 3.5 microM. WY-45,368 inhibits neither of two other metalloproteinases, thermolysin and angiotensin converting enzyme, nor does it inhibit clostridial collagenase--thus indicating specificity for mammalian collagenase.
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D000111:Acetylcysteine / Q000031:analogs & derivatives*; D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D052160:Benzothiazoles; D002460:Cell Line; D003013:Clostridium / Q000201:enzymology; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000494:pharmacology*; D051381:Rats; D012867:Skin / Q000201:enzymology; D013379:Substrate Specificity; D013623:Tail; D013710:Tendons; D013820:Thermolysin / Q000037:antagonists & inhibitors; D014357:Trypsin / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D052160:Benzothiazoles; C046256:N-((((5-chloro-2-benzothiazolyl)thio)phenyl)acetyl)cysteine; D011480:Protease Inhibitors; D014357:Trypsin; D013820:Thermolysin; D003012:Microbial Collagenase; D003545:Cysteine; D000111:Acetylcysteine