Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other gram negative bacteria.
The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.
Boulnois
G J
GJ
Varley
J M
JM
Sharpe
G S
GS
Franklin
F C
FC
D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D013045:Species Specificity
D004251:DNA Transposable Elements