Right-side-out pumping Na,K-ATPase-liposomes: a new tool to study the enzyme's receptor function.
The technology to prepare right-side-out pumping Na,K-ATPase-liposomes is described. The 50% right-side-out oriented pumps of ATP-containing liposomes are then activated by the addition of external Rb ions, leading to a ouabain-sensitive Rb-influx which is the mirror-image of the inside-out transport. The resulting internal Rb concentration is 4 to 10 fold larger than the external concentration. Finally, the accumulated Rb ions can be extruded by driving the 50% inside-out oriented pumps by external ATP.
Anner
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Moosmayer
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D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D004789:Enzyme Activation; D008081:Liposomes / Q000378:metabolism*; D008722:Methods; D010042:Ouabain / Q000494:pharmacology; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013696:Temperature
D008081:Liposomes; D010042:Ouabain; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium