Detection of antibodies to bovid herpesvirus 4 by ELISA.
An enzyme linked immunosorbent assay for antibodies to bovid herpesvirus 4 was developed using antigen prepared by detergent lysis of infected cell cultures. The assay was used to study the immune responses of experimentally-immunised calves. The results correlated well with the indirect fluorescent antibody method. A viral neutralizing antibody response could not be demonstrated in the calves.
Edwards
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Newman
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D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle / Q000382:microbiology*; D003587:Cytomegalovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay
D000914:Antibodies, Viral; D000956:Antigens, Viral