[Standardization of the conditions for measuring the specific fluorescence intensity in continuous cell cultures infected with variants of the Japanese encephalitis virus].
The use of modernized photometric accessories to a model microscope manufactured by the Leningrad Optico-Mechanical Amalgamation (USSR), as well as the practical application of innovations facilitating and standardizing research work, has made it possible to obtain objective data indicating the presence of significant, direct, linear correlation between infectious activity and the intensity of fluorescence emitted by fluorescent antibodies bound with the antigen of 11 studied variants of Japanese encephalitis virus in continuous cell lines. The study of the dynamics of fluorescence intensity permitted the objective evaluation of the previously revealed regularity in the increase of the intensity of the induced fluorescence of Japanese encephalitis antigen in continuous cell cultures.
Cherednichenko
Iu N
IuN
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D004664:Encephalitis Virus, Japanese / Q000276:immunology; D004672:Encephalitis, Japanese / Q000276:immunology / Q000382:microbiology*; D005455:Fluorescent Antibody Technique / Q000295:instrumentation; D006367:HeLa Cells / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007739:L Cells / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000592:standards
D000914:Antibodies, Viral; D000956:Antigens, Viral
Standartizatsiia usloviĭ izmereniia intensivnosti spetsificheskoĭ fliuorestsentsii v zarazhennykh variantami virusa iaponskogo éntsefalita kul'turakh perevivaemykh kletok.