title: T cell clones providing helper function for IgE synthesis release soluble factor(s) that induce IgE production in human B cells: possible role for interleukin 4 (IL-4).
abstract: We have previously demonstrated that a proportion of human T cell clones (TCC) derived from tonsil or peripheral blood (PB) of non-allergic donors, upon triggering with phytohaemagglutinin (PHA) or anti-CD3 monoclonal antibody (MoAb), were able to provide help for IgE synthesis in B cells from both allergic and non-allergic individuals. In this study we show that, upon PHA stimulation, culture supernatants from 10 selected TCC active on IgE synthesis also provided helper activity for IgE, whereas supernatants from unstimulated cultures of the same TCC were ineffective. In contrast, culture supernatants derived from five PHA-stimulated TCC, unable to provide helper function for IgE synthesis, consistently failed to elicit production of IgE. While the induction of IgE synthesis by TCC occurred in B cells from virtually all allergic and non-allergic donors, their soluble factor(s) were found to be able to provide substantial help for IgE production only in B cells from a proportion of donors tested. In addition, B cells from non-atopic donors usually appeared to be less responsive than atopic B cells to the activity of such factor(s). In contrast, synthesis of both IgG and IgM was induced in every B cell donor by both TCC and their supernatants. Partial characterization of the factor(s) providing helper function for IgE synthesis in B cells showed that it apparently had a mol. wt between 10 and 50 kD and did not bind to immobilized IgE. Such an activity appeared to be associated with the presence of interleukin 4 (IL-4) in supernatants and it was inhibited by adding both gamma-interferon and anti-human IL-4 antibody in culture.
authors.affiliation: Division of Allergology and Clinical Immunology, University of Florence, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Maggi
authors.forename: G F
authors.initials: GF
authors.lastname: Del Prete
authors.forename: A
authors.initials: A
authors.lastname: Tiri
authors.forename: D
authors.initials: D
authors.lastname: Macchia
authors.forename: P
authors.initials: P
authors.lastname: Parronchi
authors.forename: M
authors.initials: M
authors.lastname: Ricci
authors.forename: S
authors.initials: S
authors.lastname: Romagnani
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D015847:Interleukin-4; D007378:Interleukins / Q000276:immunology*; D008211:Lymphocyte Cooperation; D012130:Respiratory Hypersensitivity / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D007378:Interleukins; D015847:Interleukin-4; D007073:Immunoglobulin E