Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus.
An indirect immunofluorescence assay and a direct immunofluorescence assay were evaluated for typing clinical isolates of herpes simplex virus (HSV). The indirect immunofluorescence assay (Electro-Nucleonics, Inc.) correctly identified 16 HSV type 2 (HSV-2) isolates, but failed to identify 4 of 14 HSV-1 isolates because of background fluorescence and instability of reagents. Forty-nine HSV-1 isolates were correctly typed by direct immunofluorescence assay (Kallestad Laboratories, Inc.), but 1 of 39 HSV-2 isolates did not react with the HSV-2 type-specific antibody conjugate.
Swierkosz
E M
EM
Arens
M Q
MQ
Schmidt
R R
RR
Armstrong
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D008722:Methods; D018139:Simplexvirus / Q000145:classification* / Q000276:immunology
D000911:Antibodies, Monoclonal; D004279:DNA, Viral