Paradoxical stimulation of both lipocortin and prostaglandin production in human amnion cells by dexamethasone.
Glucocorticoids inhibit prostaglandin biosynthesis by inducing the formation of lipocortins. In human amnion cells dexamethasone elicited a concentration-dependent increase in prostaglandin production and raised intracellular lipocortin 1 concentrations. Dexamethasone could also potentiate the epidermal growth factor (EGF)-induced stimulation of prostaglandin production. EGF alone or in combination with dexamethasone increased lipocortin 1 formation in amnion cells. Human amnion cells may provide a unique insight into interactions between glucocorticoids, lipocortin and eicosanoid biosynthesis.
Mitchell
M D
MD
Department of Reproductive Medicine, University of California, San Diego, La Jolla 92093-0802.
Lytton
F D
FD
Varticovski
L
L
D000650:Amnion / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D017302:Annexins; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D015232:Dinoprostone; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D011458:Prostaglandins E / Q000096:biosynthesis*
D017302:Annexins; D006023:Glycoproteins; D011458:Prostaglandins E; D004815:Epidermal Growth Factor; D003907:Dexamethasone; D015232:Dinoprostone