Construction of a vector, pRSVcatamb38, for the rapid and sensitive assay of amber suppression in human and other mammalian cells.
We describe the generation of an amber mutation in the chloramphenicol acetyltransferase (cat) gene of the mammalian cell transfection vector pRSVcat (Gorman et.al. (1982), Proc.Natl.Acad.Sci. 79 6777-6791). We have demonstrated the in vivo suppression of this amber mutation in monkey and human cells by co-transfection with a synthetic Xenopus suppressor tRNATyr under the control of the late SV40 promoter. The vector, pRSVcatamb38, may be used to quantitate amber suppression in various mammalian cells.
Burke
J F
JF
Mogg
A E
AE
D000123:Acetyltransferases / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes; D005818:Genetic Engineering; D005822:Genetic Vectors; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D012343:RNA, Transfer / Q000235:genetics*; D013489:Suppression, Genetic
D012343:RNA, Transfer; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D004262:DNA Restriction Enzymes