Membrane polypeptide in rabbit erythrocytes associated with the inhibition of L-lactate transport by a synthetic anhydride of lactic acid.
The synthetic lactyl anhydride isobutylcarbonyl lactyl anhydride (iBCLA), a selective and potent inhibitor of L-(+)-lactate transport in rabbit erythrocytes, reduces the chemical labeling of a 40-50-kdalton polypeptide by tritiated 4,4'-diisothiocyanato-2,2'-dihydrostilbenedisulfonate ([3H]H2DIDS). iBCLA does so in a dose-dependent manner at concentrations that strongly inhibit lactate lactate exchange but not chloride-phosphate exchange. These labeling experiments and inhibition reversal studies using iBCLA, p-(chloro-mercuri)benzenesulfonic acid (pCMBS), and dithiothreitol (DDT) suggest that iBCLA does not act at sulfhydryl groups but at or near an amino group that is near a disulfide linkage in the polypeptide which catalyzes lactate transport. These experiments support the association between specific monocarboxylate transport and a 40-50-kdalton membrane-bound polypeptide of the rabbit erythrocyte.
Donovan
J A
JA
Jennings
M L
ML
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D007773:Lactates / Q000097:blood* / Q000494:pharmacology*; D019344:Lactic Acid; D008565:Membrane Proteins / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology*; D008970:Molecular Weight; D027501:Monocarboxylic Acid Transporters; D011817:Rabbits; D014316:Tritium
D002352:Carrier Proteins; D007773:Lactates; D008565:Membrane Proteins; D027501:Monocarboxylic Acid Transporters; D014316:Tritium; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D019344:Lactic Acid; D002731:4-Chloromercuribenzenesulfonate; C026807:isobutylcarbonyllactyl anhydride; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D004229:Dithiothreitol