A site-specific endonuclease from Pseudomonas aeruginosa.
PaeI, a new restriction endonuclease from Pseudomonas aeruginosa clinical strain was isolated and characterized. It recognizes and cleaves the sequence 5'-GCATG reduced C-3' generating DNA fragments with 3'-tetranucleotide sticky ends. DNAs of pBR322, SV40 and bacteriophage lambda have one, two and six PaeI recognition sites, respectively. Seventy-two strains of Pseudomonas, Clostridium, Escherichia coli, Shigella, Proteus and Saccharomyces were screened for the presence of site-specific endonucleases. Here we describe the PaeI restriction enzyme found in Pseudomonas aeruginosa; other data will be published elsewhere. Earlier Hinkle and Miller isolated from P. aeruginosa a PaeR7 restriction endonuclease recognizing and cleaving a sequence 5'-C reduced TCGAG-3' (1). Sequence analysis of DNAs cleaved by PaeI shows that the enzyme is the isoschizomer of SphI (2).
Sokolov
N N
NN
Fitsner
A B
AB
Anikeitcheva
N V
NV
Choroshoutina YuB
Samko
O T
OT
Kolosha
V O
VO
Fodor
I
I
Votrin
I I
II
D001483:Base Sequence; D001665:Binding Sites; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013379:Substrate Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C046184:endodeoxyribonuclease PaeI; D015252:Deoxyribonucleases, Type II Site-Specific