Study of oncogenic potentialities of human melanoma: identification of N-ras oncogene after DNA transfer and tumour induction.
The oncogenic potentialities of human melanoma cells derived from two different patients were studied using DNA-mediated gene transfer into NIH 3T3 cells followed by tumor induction into athymic nude nice. 64% of the mice injected subcutaneously with selected cells which had been co-transfected with human melanoma DNA and the selective marker NeoR developed tumors within 3-4 weeks, while up to 100% of those injected with cells transfected three days before with melanoma DNA developed tumors within 4-6 weeks. Southern blots analysis of the tumors indicated that almost all of them contained human sequences. Hybridization with different oncogene probes showed the presence of an human Eco RI N-ras-hybridizing fragment in the primary and secondary derived tumors, indicating that a transforming N-ras oncogene in human melanoma had been transferred to recipient cells and that transformed cells induced tumors in nude mice.
Jouanneau
J
J
U248 INSERM Faculté de Médecine Lariboisière-Saint-Louis, Paris, France.
Bertrand
S
S
Ducros
E
E
Longuet
M
M
Dore
J F
JF
Tavitian
A
A
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D011905:Genes, ras; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D051379:Mice; D009447:Neuroblastoma / Q000235:genetics; D014162:Transfection
D004273:DNA, Neoplasm