title: Demonstration of a ouabainlike plasma compound in hypertension prone and hypertension resistant rats.
abstract: Material extracted and partially purified from plasma of the Sabra hypertension prone rats was found to be capable of 1) inhibiting the binding of 3H-ouabain to rat brain synaptosomes, 2) inhibiting the activity of rat brain microsomal Na, K activated adenosine triphosphatase, and 3) increasing the contractile force of rat heart muscle. The results demonstrate the presence of a ouabainlike compound in the plasma of these rats. The plasma concentration of this compound in Sabra hypertension prone rats was 698 +/- 199 nmol/ml in ouabain equivalents (SEM; n = 11) versus 2543 +/- 1140 nmol/ml (n = 9) in the Sabra normotensive strain. The presence of ouabainlike compound in the plasma is consistent with the hypothesis that this compound functions as a hormone that regulates Na, K activated adenosine triphosphatase activity and the physiological processes in which this enzyme is involved.
authors.forename: D
authors.initials: D
authors.lastname: Lichtstein
authors.forename: D
authors.initials: D
authors.lastname: Minc
authors.forename: Y
authors.initials: Y
authors.lastname: Shimoni
authors.forename: J
authors.initials: J
authors.lastname: Deutsch
authors.forename: J
authors.initials: J
authors.lastname: Mekler
authors.forename: D
authors.initials: D
authors.lastname: Ben-Ishay
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006973:Hypertension / Q000097:blood*; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D010042:Ouabain / Q000097:blood* / Q000494:pharmacology; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014316:Tritium
chemical_list: D014316:Tritium; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase