An immunocytochemical method for assaying oestrogen receptors in breast cancers. A comparison with the steroid binding assay.
The presence of oestrogen receptors was studied in 105 human breast carcinomas using monoclonal antibodies (Abbott ER-ICA kit). The oestrogen receptors of neoplastic cells were semiquantitatively measured and correlations were made to receptor values determined by a dextran-coated charcoal (DCC) steroid binding assay and to histological grade. Immunoreactive cells were found in about 2/3 of the tumours. Usually only a fraction of the cells within each tumour were immunoreactive, and the staining intensity varied among different cells. In general, well differentiated tumours had a greater proportion of immunoreactive cells than poorly differentiated ones. In most cases (65/98) a good agreement was found between the ER-ICA method and the DCC assay. However, in 33 cases discrepancies were demonstrated.
Ottestad
L O
LO
Department of Biochemistry, Norwegian Radium Hospital, Oslo.
Tveit
K M
KM
Nesland
J M
JM
Dybsjord
I
I
Børmer
O
O
Høie
J
J
Pihl
A
A
D001943:Breast Neoplasms / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D011960:Receptors, Estrogen / Q000032:analysis*; D013256:Steroids / Q000378:metabolism
D011960:Receptors, Estrogen; D013256:Steroids