title: Adoptive cell transfer studies to examine the role of T lymphocytes in immunity to Trypanosoma musculi.
abstract: Previous studies in this laboratory utilizing monoclonal antibody-induced immunosuppression have demonstrated that the T-helper lymphocyte is primarily responsible for the T lymphocyte dependency of Trypanosoma musculi elimination from the bloodstream of mice, and that T-cytotoxic lymphocytes play a minimal role in this response. In the current study, these findings were extended by examining the effects of adoptive cell transfers on the course of infection with T. musculi using immune splenocytes enriched for T lymphocyte subpopulations. These studies demonstrated that adoptive transfer of immune splenic T lymphocytes resulted in a specific, dose-related enhancement of kinetics of trypanosome elimination. This effect was found to be due to the presence of L3T4+ T-helper cells in the immune splenocyte population. Adoptive transfer of Lyt-2+ T-cytotoxic cells or lymphokine-activated killer (LAK) cells was ineffective in altering the course of infection. In addition, it was found that immune B lymphocytes were equally capable of adoptively transferring immunity to T. musculi, suggesting that the primary role of the T-helper lymphocyte is to provide help in the induction of parasite-specific antibodies.
authors.affiliation: Department of Cellular and Molecular Toxicology, Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709.
authors.forename: R V
authors.initials: RV
authors.lastname: House
authors.forename: J H
authors.initials: JH
authors.lastname: Dean
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001402:B-Lymphocytes / Q000502:physiology / Q000637:transplantation; D005260:Female; D007116:Immunization, Passive; D007694:Killer Cells, Natural / Q000502:physiology / Q000637:transplantation; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology* / Q000637:transplantation; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology* / Q000637:transplantation; D014352:Trypanosomiasis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte