In vitro metabolism of 7 alpha-methyl-19-nortestosterone by rat liver, prostate, and epididymis.
The in vitro metabolism of 7 alpha-methyl-19-nortestosterone (7 alpha-methyl-3-oxo-estr-4-ene-17 beta-ol) was investigated in male rat liver, ventral prostate, and epididymis. Three metabolites were recovered from the liver incubation: 7 alpha-methyl-estr-4-ene-3,17-dione, 7 alpha-methyl-5 beta-estrane-3,17 beta-diol, and 7 alpha-methyl-3-oxo-estr-4-ene-16,17 beta-diol, whereas prostate and epididymis did not perceptibly metabolize the steroid. The kinetics of metabolism in liver differed significantly from those of testosterone or 19-nortestosterone. About 85% of the 7 alpha-methyl-19-nortestosterone added was recovered at the end of 30 min of incubation. We conclude that the androgenic potency of 7-alpha-methyl-19-nortestosterone is not dependent on its further conversion to 5 alpha-dihydro-metabolites.
Agarwal
A K
AK
Population Council, Center for Biomedical Research, New York, New York 10021.
Monder
C
C
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004822:Epididymis / Q000378:metabolism*; D004963:Estrenes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004963:Estrenes