Molecular cloning of murine p68, a Ca2+-binding protein of the lipocortin family.
A plasmid cDNA library prepared from a T-lymphocyte clone of murine strain B10.A origin was screened by cross-species DNA hybridisation using a partial human p68 cDNA clone, identified as containing coding sequences for previously determined amino-acid sequences. The longest p68 cDNA insert from this library and a full-length cDNA insert from a second similar library were fully sequenced. A comparison of the derived amino-acid sequence with that of human p68 revealed extensive homology (95% overall). Homology at the nucleotide level was 89% in the open reading frame and 85% and 50% in the 5' (33 nucleotides) and 3' (347 nucleotides) non-coding regions respectively. Eight segments of internal homology were observed, each containing a highly conserved consensus region of 17 amino acids correlating with that described for several membrane associated calcium-binding proteins [Geisow, M. J., Fritsche, U., Hexham, J. M. & Johnson, T. (1986) Nature (Lond.) 320, 636-638]. These results provide further evidence that p68 is a member of the same gene family as p32,p36 and lipocortin I and demonstrate an unusually high level of inter-species sequence conservation of p68 between mouse and human.
Moss
S E
SE
Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London, England.
Crompton
M R
MR
Crumpton
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D017302:Annexins; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D017302:Annexins; D002135:Calcium-Binding Proteins; D006023:Glycoproteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D004247:DNA