title: [Experimental conditions of a technic of in vitro transcription using placental nuclei].
abstract: To examine the expression of human placental lactogen, we investigated transcription in isolated placental nuclei. Using SH-agarose affinity chromatography, we have isolated Hg-RNA synthetized in vitro. These RNA are translated in a wheat germ cell-free system. hPL synthetized in vitro, accounting for 20% of total protein. This system should prove useful for the study of gene expression of hPL.
authors.affiliation: Laboratoire de Biochimie Médicale I, Faculté de Médecine, Vandaeuvre-les-Nancy.
authors.forename: K
authors.initials: K
authors.lastname: Hess
authors.forename: H
authors.initials: H
authors.lastname: el Ouardirhi
authors.forename: F
authors.initials: F
authors.lastname: Belleville
authors.forename: P
authors.initials: P
authors.lastname: Paysant
authors.forename: P
authors.initials: P
authors.lastname: Nabet
mesh_terms: D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008722:Methods; D010928:Placental Lactogen / Q000235:genetics*; D011247:Pregnancy; D014176:Protein Biosynthesis; D015138:RNA, Nuclear / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D015138:RNA, Nuclear; D010928:Placental Lactogen
vernacular_title: Etude des conditions expérimentales d'une technique de transcription in vitro utilisant des noyaux de placenta.