title: Phosphorylation of fodrin (nonerythroid spectrin) by the purified insulin receptor kinase.
abstract: Fodrin (nonerythroid spectrin) from porcine brain was found to be phosphorylated on tyrosine residues by the purified insulin receptor kinase. The phosphorylation occurred in an insulin-sensitive manner with a physiologically relevant km. The beta(235 K) subunit of fodrin, but not the alpha(240 K) subunit, was phosphorylated by the kinase. Neither the alpha(240 K) subunit nor the beta(220 K) subunit of erythrocyte spectrin was phosphorylated under the same conditions. Fodrin phosphorylation by the purified insulin receptor kinase was markedly inhibited by F-actin. These data raise the possibility that tyrosine phosphorylation of fodrin plays some roles in the regulation of plasma membrane-microfilament interaction.
authors.forename: T
authors.initials: T
authors.lastname: Kadowaki
authors.forename: E
authors.initials: E
authors.lastname: Nishida
authors.forename: M
authors.initials: M
authors.lastname: Kasuga
authors.forename: T
authors.initials: T
authors.lastname: Akiyama
authors.forename: F
authors.initials: F
authors.lastname: Takaku
authors.forename: M
authors.initials: M
authors.lastname: Ishikawa
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: S
authors.initials: S
authors.lastname: Kathuria
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita-Yamaguchi
mesh_terms: D000199:Actins / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000378:metabolism*; D002384:Catalysis; D002417:Cattle; D006801:Humans; D066298:In Vitro Techniques; D008840:Microfilament Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011972:Receptor, Insulin; D013049:Spectrin / Q000378:metabolism; D013552:Swine; D014443:Tyrosine / Q000378:metabolism
chemical_list: D000199:Actins; D000596:Amino Acids; D002352:Carrier Proteins; D008840:Microfilament Proteins; D011494:Protein Kinases; D011972:Receptor, Insulin; D013049:Spectrin; D014443:Tyrosine