Reconstitution of the gastrointestinal microflora of lactobacillus-free mice.
A colony of mice that do not harbor lactobacilli in their digestive tracts but whose intestinal microflora is otherwise functionally similar to that of conventional animals was derived. Methods used to reconstitute the intestinal microflora of the mice included inoculation of the animals with cultures of specific microbes, noncultivable microbes attached to epithelial cells, and cecal contents from conventional mice treated with chloramphenicol. Twenty-six microflora-associated characteristics were monitored by using relatively simple tests to determine the microflora status of the mice.
Tannock
G W
GW
Department of Microbiology, University of Otago, Dunedin, New Zealand.
Crichton
C
C
Welling
G W
GW
Koopman
J P
JP
Midtvedt
T
T
D000818:Animals; D001419:Bacteria / Q000201:enzymology / Q000302:isolation & purification; D002432:Cecum / Q000201:enzymology / Q000382:microbiology; D004064:Digestive System / Q000201:enzymology / Q000382:microbiology*; D005856:Germ-Free Life; D007778:Lactobacillus / Q000302:isolation & purification* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010447:Peptide Hydrolases / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms
D010447:Peptide Hydrolases