Alkaline phosphatase promotes radioprotection and accumulation of WR-1065 in V79-171 cells incubated in medium containing WR-2721.
Addition of alkaline phosphatase and WR-2721 to culture medium containing V79-171 cells leads to production of WR-1065 and its disulphide forms in the medium, to cellular accumulation of WR-1065, and to radioprotection which correlates with cellular WR-1065 level.
Calabro-Jones
P M
PM
Fahey
R C
RC
Smoluk
G D
GD
Ward
J F
JF
D000469:Alkaline Phosphatase / Q000494:pharmacology*; D004999:Amifostine / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D002588:Cesium Radioisotopes; D006224:Cricetinae; D003412:Cricetulus; D005720:Gamma Rays; D066298:In Vitro Techniques; D008624:Mercaptoethylamines / Q000378:metabolism* / Q000494:pharmacology; D009946:Organothiophosphorus Compounds / Q000378:metabolism*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D002588:Cesium Radioisotopes; D008624:Mercaptoethylamines; D009946:Organothiophosphorus Compounds; D011837:Radiation-Protective Agents; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane; D000469:Alkaline Phosphatase; D004999:Amifostine