title: A comparative analysis of radiation- and virus-induced leukemias in BALB/c mice.
abstract: Endogenous murine leukemia virus (MuLV) proviral copies were analyzed in thymomas induced in normal BALB/c (Fv-1b) and in Fv-1n congenic mice by X-irradiation. Both strains of mice developed leukemia with similar kinetics, indicating that N-tropism of endogenous MuLV was not a rate-limiting factor in development of disease. Southern blot analysis, using a probe specific for ecotropic virus and for ecotropic-specific sequences retained in pathogenic, env-recombinant viruses, showed that the majority of radiation leukemias lacked newly acquired, clonally integrated, proviruses. This was in contrast to virus-induced leukemias, which routinely exhibited several new proviral integration sites. When an internal proviral DNA restriction fragment was monitored, some radiation leukemias showed evidence of nonclonal infection, accounting for more frequent isolation of infectious virus from such leukemias. Differences in expression of T-cell surface antigens were found in X-ray-induced and virus-induced leukemias. All radiation leukemias were TL positive, whereas virus-induced leukemias were primarily negative for TL. Some differences were also found in Lyt-1 and Lyt-2 expression. The data as a whole suggest that, in the majority of cases, radiation leukemogenesis is not initiated by a viral route--that is, the sort of viral mechanism for which exogenous infection by known pathogenic MuLV is the paradigm.
authors.forename: E W
authors.initials: EW
authors.lastname: Newcomb
authors.forename: R
authors.initials: R
authors.lastname: Binari
authors.forename: E
authors.initials: E
authors.lastname: Fleissner
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D009052:Leukemia Virus, Murine / Q000472:pathogenicity; D007942:Leukemia, Experimental / Q000382:microbiology* / Q000503:physiopathology; D007953:Leukemia, Radiation-Induced / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology; D013945:Thymoma / Q000382:microbiology / Q000503:physiopathology*; D013953:Thymus Neoplasms / Q000382:microbiology / Q000503:physiopathology*; D014965:X-Rays
chemical_list: D000954:Antigens, Surface; D004273:DNA, Neoplasm