Coupled endo-exodeoxyribonuclease activity from HeLa cell nuclei interacts with specific regions of the SV40 mini chromosome.
A recently described endo-exodeoxyribonuclease activity (1) was determined to interact preferentially with the origin/enhancer region and the 5' end of the late promoter region of the SV40 mini chromosome. The enzymatic activity has no preferential site of interaction with naked DNA, which suggests, the chromatin organization is responsible for this site directed interaction.
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D001665:Binding Sites; D002467:Cell Nucleus / Q000201:enzymology; D002843:Chromatin / Q000378:metabolism; D002874:Chromosome Mapping; D002875:Chromosomes / Q000378:metabolism*; D004256:DNA Polymerase I; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D011485:Protein Binding; D013539:Simian virus 40 / Q000378:metabolism*
D002843:Chromatin; D004279:DNA, Viral; D004256:DNA Polymerase I; D004720:Endonucleases; D005092:Exonucleases; D004262:DNA Restriction Enzymes; C020104:endoexonuclease