Cytomegalovirus early and late membrane antigens detected by antibodies in human convalescent sera.
Using indirect immunofluorescence and a panel of human convalescent-phase sera, we identified cytomegalovirus (CMV) early and late membrane antigens (CMV-EMA and CMV-LMA, respectively) as separate entities on the surfaces of viable CMV-infected fibroblasts starting at 6 to 12 and 36 to 48 h postinoculation, respectively. For expression of CMV-EMA and CMV-LMA, infectious virus and active protein synthesis were required, whereas the expression of CMV-LMA, in addition, required viral DNA synthesis. Our data suggest that CMV-EMA and CMV-LMA form an individual set of CMV antigens that are different from intracellular CMV antigens and possibly (partly) different from the viral envelope.
Middeldorp
J M
JM
Jongsma
J
J
The
T H
TH
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology; D013997:Time Factors
D000914:Antibodies, Viral; D000956:Antigens, Viral; D008565:Membrane Proteins