Assignment of aromatic spin systems in the 1H nuclear magnetic resonance spectrum of adenylate kinase.
A combination of selective spin decoupling, two-dimensional double quantum spectroscopy, correlated spectroscopy (COSY), and pH titration experiments brought about the assignment of all tyrosyl spin systems and completed the assignment of the histidyl spin systems in porcine adenylate kinase. In the detection of the tyrosyl spin systems it proved to be advantageous to resort to the COSY method rather than to two-dimensional double quantum spectroscopy. In the titration experiments, His189 revealed a second apparent pK value at pH 8.3, which is explained by deprotonation of the adjacent residue Cys187. None of the seven tyrosyl side-chains shows any evidence for deprotonation up to the point of denaturation of the protein, which took place around pH 10.
Rösch
P
P
Gross
K H
KH
D000263:Adenylate Kinase; D000595:Amino Acid Sequence; D000818:Animals; D006639:Histidine; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010770:Phosphotransferases; D013552:Swine
D006639:Histidine; D010770:Phosphotransferases; D000263:Adenylate Kinase