title: Regulation of the trehalose-6-phosphate synthase complex in Saccharomyces. I. Interconversion of forms by phosphorylation.
abstract: Trehalose-6-phosphate synthase is another example of an enzyme of carbohydrate metabolism, in Saccharomyces, which could be regulated by interconversion of forms. Deactivation was mediated both in vivo and in vitro by a cyclic AMP-dependent protein kinase. Reversibility of this process was obtained by a phosphatase treatment leading to an increase in activity. The phosphorylated, less active form of the enzyme proved to be more susceptible to activation by ATP.Mg. Mutants with well defined lesions in the cyclic AMP-dependent protein kinase system were used to corroborate our findings of a possible regulatory mechanism of trehalose-6-phosphate synthase activity by interconversion of forms.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, CCMN, Universidade Federal do Rio de Janeiro, Brasil.
authors.forename: A C
authors.initials: AC
authors.lastname: Panek
authors.forename: P S
authors.initials: PS
authors.lastname: de Araujo
authors.forename: V
authors.initials: V
authors.lastname: Moura Neto
authors.forename: A D
authors.initials: AD
authors.lastname: Panek
mesh_terms: D005796:Genes; D005800:Genes, Fungal; D005809:Genes, Regulator; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D009097:Multienzyme Complexes / Q000235:genetics*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity
chemical_list: D009097:Multienzyme Complexes; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase; D011494:Protein Kinases