Characterization of ferrioxamine E as the principal siderophore of Erwinia herbicola (Enterobacter agglomerans).
Several strains of the enterobacterial group Erwinia herbicola (Enterobacter agglomerans) were screened for siderophore production. After 3 days of growth in a low-iron medium, all strains studied produced hydroxamate siderophores. The retention values of the main siderophore during thin-layer chromatography on silica gel plates and on HPLC reversed-phase columns were identical with those of an authentic sample of ferrioxamine E (norcardamine). Gas-chromatographic analysis of the HI hydrolyzate yielded succinic acid and 1,5-diaminopentane in equimolar amounts; fast-atom-bombardment (FAB) mass spectroscopy showed a molecular mass of 653 Da. Iron from 55Fe-labelled ferrioxamine E was well taken up by iron-starved cells of E. herbicola (Km = 0.1 microM, Vmax = 8 pmol mg-1 min-1). However, besides ferrioxamine E (100%), several exogenous siderophores such as enterobactin (94.5%), ferric citrate (78.5%), coprogen (63.5%) and ferrichrome (17.5%) served as siderophores, suggesting the presence of multiple siderophore receptors in the outer membrane of E. herbicola.
Berner
I
I
Institut für Biologie I, Universität Tübingen, Federal Republic of Germany.
Konetschny-Rapp
S
S
Jung
G
G
Winkelmann
G
G
D001693:Biological Transport, Active; D002851:Chromatography, High Pressure Liquid; D004885:Erwinia / Q000378:metabolism*; D005290:Ferric Compounds / Q000302:isolation & purification / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000378:metabolism*; D017262:Siderophores
D005290:Ferric Compounds; D007502:Iron Chelating Agents; D010456:Peptides, Cyclic; D017262:Siderophores; C017508:ferrioxamine E; D007501:Iron