Phosphofructokinase activity in fibroblasts from patients with Alzheimer's disease and age- and sex-matched controls.
The activity of the enzyme phosphofructokinase (PFK) was comparable in cultured skin fibroblasts from eight patients with Alzheimer's disease and eight age- and sex-matched controls. Mean activities were similar in the two groups whether measured under nonallosteric conditions at pH 8.0 or under allosteric conditions at pH 7.0, in the presence of 0.1 or 1 mM ATP. Activities of PFK in Alzheimer's disease and control cells also showed a similar temperature dependence and similar isozyme patterns on column chromatography. These results argue against the existence of significant structural variations of PFK in Alzheimer's disease.
Sims
N R
NR
Altschul Laboratory for Dementia Research, Cornell University Medical College, White Plains, New York 10605.
Blass
J P
JP
D000255:Adenosine Triphosphate / Q000378:metabolism; D000368:Aged; D000544:Alzheimer Disease / Q000201:enzymology* / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D013696:Temperature
D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1