In vitro effect of estrogens on the peroxidase activity of human endometrium.
The effect of estrogen on the induction of human endometrial peroxidase was studied in vitro. When 17 beta-estradiol was added to the incubation medium, endometrial peroxidase activity was markedly increased, and the highest enzymatic activities were reached at about 12 h of incubation. The ability of estrogen and related compounds to increase peroxidase activity at a concentration of 10(-7) M was 17 beta-estradiol greater than estrone greater than 17 alpha-ethynylestradiol greater than estriol = diethylstilbestrol. Mestranol, progesterone, and testosterone had almost no activity. The induction of peroxidase by 17 beta-estradiol was inhibited by actinomycin D or cycloheximide. This method may be useful for determining the direct estrogenic effect of compounds in human endometrium.
Okada
H
H
Nawa
M
M
Kimura
J
J
D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000494:pharmacology; D004717:Endometrium / Q000201:enzymology*; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D004964:Estriol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D004970:Estrone / Q000494:pharmacology; D004997:Ethinyl Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D008656:Mestranol / Q000494:pharmacology; D009195:Peroxidase; D010544:Peroxidases / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology
D004967:Estrogens; D007527:Isoenzymes; D003609:Dactinomycin; D004970:Estrone; D013739:Testosterone; D004997:Ethinyl Estradiol; D011374:Progesterone; D004958:Estradiol; D004054:Diethylstilbestrol; D003513:Cycloheximide; D008656:Mestranol; D010544:Peroxidases; D009195:Peroxidase; D004964:Estriol