Serial transplantation of a heterologous, mixed Müllerian tumor in the nude (athymic) mouse: growth characteristics and morphology.
In view of the poor prognosis for patients with mixed Müllerian tumors, transplantation of these tumors into nude mice offers an important model for the study of the physiologic processes occurring in this type of malignancy. We report the growth and serial transplantation of all elements of this tumor in the nude mouse. The lag period for establishment of the original tumor was 5 weeks. The tumor required 11 weeks to achieve a 2 X 2 X 1 cm volume at the initial pass, but on subsequent passes reached this volume in 6-12 weeks (mean = 8.3). Microscopic examination revealed that the epithelial, stromal and heterologous elements were serially transplanted. An osteoid matrix, confirmed by special staining for bone, was identifiable at both the light and electron microscopic levels. Throughout successive passages, the tumor retained the morphologic features of the original tumor. This tumor line is stable and reproducible, providing a readily available source of this ovarian carcinoma for clinical research studies.
Centola
G M
GM
Bibro
M C
MC
Hoskins
W J
WJ
D000230:Adenocarcinoma / Q000473:pathology; D000368:Aged; D000818:Animals; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009373:Neoplasms, Germ Cell and Embryonal / Q000473:pathology* / Q000648:ultrastructure; D010051:Ovarian Neoplasms / Q000473:pathology