title: Plasmid genes required for microcin B17 production.
abstract: The production of the antibiotic substance microcin B17 (Mcc) is determined by a 3.5-kilobase DNA fragment from plasmid pMccB17. Several Mcc- mutations on plasmid pMccB17 were obtained by both transposon insertion and nitrosoguanidine mutagenesis. Plasmids carrying these mutations were tested for their ability to complement Mcc- insertion or deletion mutations on pMM102 (pMM102 is a pBR322 derivative carrying the region encoding microcin B17). Results from these experiments indicate that at least four plasmid genes are required for microcin production.
authors.forename: J L
authors.initials: JL
authors.lastname: San Millán
authors.forename: R
authors.initials: R
authors.lastname: Kolter
authors.forename: F
authors.initials: F
authors.lastname: Moreno
mesh_terms: D001430:Bacteriocins / Q000096:biosynthesis*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005838:Genotype; D009154:Mutation; D010957:Plasmids; D013045:Species Specificity
chemical_list: D001430:Bacteriocins; C020411:microcin; D004262:DNA Restriction Enzymes