Identification of the protein product of the c-mos proto-oncogene in mouse testes.
The mouse c-mos proto-oncogene RNA is expressed primarily in mouse gonadal tissues and embryos. Until now, the c-mos protein has not been identified. Utilizing two different site-directed affinity purified anti-peptide antibodies, we have identified a 43 kDa c-mos protein in mouse testes and in germ cell preparations derived from testes. This 43 kDa testicular protein was found to be structurally related to a bacterially expressed c-mos protein by peptide mapping. Immunoblots of whole mouse sections were employed to establish that the c-mos protein is expressed primarily in the testes.
Herzog
N K
NK
Department of Molecular Pathology, University of Texas, MD and Anderson Cancer Center, Houston 77030.
Singh
B
B
Elder
J
J
Lipkin
I
I
Trauger
R J
RJ
Millette
C F
CF
Goldman
D S
DS
Wolfes
H
H
Cooper
G M
GM
Arlinghaus
R B
RB
D000818:Animals; D008297:Male; D051379:Mice; D008970:Molecular Weight; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000276:immunology; D017231:Proto-Oncogene Proteins c-mos; D013094:Spermatozoa / Q000032:analysis; D013737:Testis / Q000032:analysis*
D011518:Proto-Oncogene Proteins; D017231:Proto-Oncogene Proteins c-mos