Islet beta-cell function and polymorphism in the 5'-flanking region of the human insulin gene.
The present study investigates the possible relationship between human beta-cell secretory capacity and polymorphism in the 5'-flanking region of the human insulin gene. The glucose potentiation slope was measured in normal and non-insulin-dependent diabetic subjects (NIDDM). This slope, as reported previously (Ward, W. K., et al., Am. J. Physiol. 1984; 246:E405-11), is an index of the ability of hyperglycemia to potentiate the insulin response to arginine and as such is a measure of beta-cell responsiveness to glucose. Restriction enzyme analysis using a human insulin gene probe was performed on leukocyte DNA isolated from the same individuals. We conclude that a 1.6 kb polymorphism in the 5'-flanking region of the human insulin gene in both normal and NIDDM subjects has no association with insulin secretory responses as defined here by the glucose potentiation slope.
Permutt
M A
MA
Rotwein
P
P
Andreone
T
T
Ward
W K
WK
Porte
D
D
D000328:Adult; D000483:Alleles; D001120:Arginine; D001786:Blood Glucose / Q000378:metabolism; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D007328:Insulin / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008875:Middle Aged; D011110:Polymorphism, Genetic
D001786:Blood Glucose; D007328:Insulin; D001120:Arginine; D004262:DNA Restriction Enzymes