Changes in heparan sulfate pattern but not in oncogene expression correlate with tumor growth in spontaneous transformation of cells.
For the clonal analysis of spontaneous malignant transformation of cells, a clonal cell line of low tumorigenicity, derived from a mouse embryo mass culture, was injected into syngeneic animals in a sufficient dose (10(7) cells) to produce tumors. Cell lines and clones produced from several such tumors had acquired a 10(4)- to 10(5)-fold higher level of tumorigenicity. Anchorage-independent growth did not co-select with tumorigenicity. There was no evidence for overexpression of proto-oncogenes in the highly tumorigenic clones; p53 protein and mRNA levels were also essentially equal and low. There was a specific structural difference in the O-sulfate residues in oligosaccharides of heparan sulfates in all the tumor cell lines when compared with their parent clone, similar to that observed before in SV40 virus-induced cell transformation (Winterbourne and Mora, 1978).
Smith
C A
CA
Division of Cancer Biology and Diagnosis, National Cancer Institute, Bethesda, Maryland 20892.
Winterbourne
D J
DJ
McFarland
V W
VW
Mora
P T
PT
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002999:Clone Cells; D006001:Glycoconjugates / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000378:metabolism*; D009857:Oncogenes; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000378:metabolism; D016159:Tumor Suppressor Protein p53
D006001:Glycoconjugates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53