Behaviour of guinea pig T cells stimulated by antigen, allo-antigen and mitogen.
The expression of major histocompatibility (MHC) antigens on guinea pig T cells was used as a functional marker for lymphocyte activation. Antigen-stimulated lymphocytes were recovered from guinea pigs responding to the contact sensitizer DNFB, and isolated T cells were then phenotyped using a new antiguinea pig monoclonal antibody, MSgp7. The level of expression of MHC class II, as defined by the monclonal antibody, MSgp8, was increased on T cells recovered 4 days after sensitization, as compared with unsensitized controls. The value of this experiment was extended by measuring MHC class II expression on T cells stimulated in vitro by the mitogen concanavalin A, where a clear increase in MSgp8 binding was also observed. Confirmation of the specificity of MSgp8 for guinea pig MHC class II antigens was achieved by studying the inhibitory capacity of this antibody on an MHC class II restricted mixed leucocyte reaction. The combination of antibodies MSgp7 and MSgp8 with flow cytometry could be applied to other guinea pig experimental models to quantitate the expression of MHC class II antigens on T cells to determine their putative value in disease manifestation.
Healey
D G
DG
Department of Pathology, Royal College of Surgeons of England, London.
Agha
N
N
Turk
J L
JL
D000818:Animals; D000911:Antibodies, Monoclonal; D003208:Concanavalin A; D004139:Dinitrofluorobenzene / Q000276:immunology*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000032:analysis / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007519:Isoantigens; D009578:Nitrobenzenes; D003208:Concanavalin A; D004139:Dinitrofluorobenzene