High expression of an N-acetylglucosaminyltransferase III in 3'-methyl DAB-induced hepatoma and ascites hepatoma.
Ascites hepatoma AH-66 and 3'-Me-DAB-induced hepatoma of rats contain highly active N-acetylglucosaminyltransferase III (GnT-III) which catalyzes the addition of N-acetylglucosamine through a beta 1-4 linkage (bisecting N-acetylglucosamine) to the beta-linked mannose of the trimannosyl core of asparagine-linked sugar chains, whereas normal rat liver contains very little. The high activity was also detected in fetal rat liver, newborn rat liver, hyperplastic nodules and various transplantable hepatomas.
Nishikawa
A
A
Department of Biochemistry, Osaka University Medical School, Japan.
Fujii
S
S
Sugiyama
T
T
Hayashi
N
N
Taniguchi
N
N
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005260:Female; D005333:Fetus; D005964:Glucosyltransferases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000254:growth & development; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008115:Liver Regeneration; D008297:Male; D008749:Methyldimethylaminoazobenzene; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases; D009844:Oligosaccharides / Q000096:biosynthesis; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D009844:Oligosaccharides; D008749:Methyldimethylaminoazobenzene; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C054599:beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase