Chitin-coated Celite as an affinity adsorbent for high-performance liquid chromatography of lysozyme.
Preparation of chitin-coated Celite as an affinity adsorbent for high-performance liquid chromatography of lysozymes and its application to separation of N-bromosuccinimide-oxidized lysozymes are described. By pH gradient elution, two diastereomers of oxindolealanine-62-lysozyme, delta 1-acetoxytryptophan-62-lysozyme (intermediate product in the reaction in acetate buffer), and native lysozyme were all separated within 40 min.
Yamada
H
H
Fukumura
T
T
Ito
Y
Y
Imoto
T
T
D000818:Animals; D002645:Chickens; D002686:Chitin; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007692:Diatomaceous Earth; D004529:Egg White; D009113:Muramidase / Q000302:isolation & purification*; D012822:Silicon Dioxide
D002686:Chitin; D007692:Diatomaceous Earth; D012822:Silicon Dioxide; D009113:Muramidase