title: "Retroposon" insertion into the cellular oncogene c-myc in canine transmissible venereal tumor.
abstract: We examined by Southern blotting the state of the cellular oncogene c-myc in the dog transmissible venereal tumor. The tumor DNA contains a 16.8-kilobase pair (kbp) rearranged c-myc fragment in addition to the normal 15-kbp and 7.5-kbp fragments. We compared the structure of the cloned rearranged c-myc (re-myc) with that of a cloned normal c-myc and found that the rearrangement was due to the insertion of a 1.8-kbp DNA upstream to the first exon of c-myc. The inserted DNA is flanked by 10-base-pair direct repeats and contains a dA-rich tail, suggesting its origin from mRNA. Partial sequence of the inserted element showed 62% homology with the primate interdispersed Kpn I repetitive element. These results provide an example for the behavior of repetitive DNA sequences like the Kpn I family, as movable elements that can transpose nearby to oncogenes or other structural genes and perhaps affect their activity.
authors.forename: N
authors.initials: N
authors.lastname: Katzir
authors.forename: G
authors.initials: G
authors.lastname: Rechavi
authors.forename: J B
authors.initials: JB
authors.lastname: Cohen
authors.forename: T
authors.initials: T
authors.lastname: Unger
authors.forename: F
authors.initials: F
authors.lastname: Simoni
authors.forename: S
authors.initials: S
authors.lastname: Segal
authors.forename: D
authors.initials: D
authors.lastname: Cohen
authors.forename: D
authors.initials: D
authors.lastname: Givol
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004273:DNA, Neoplasm / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D009857:Oncogenes; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014685:Venereal Tumors, Veterinary / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004273:DNA, Neoplasm