Purification and characterization of a human T-lymphocyte-derived glial growth-promoting factor.
Antigen- or lectin-stimulated T lymphocytes and human T-cell leukemia virus (HTLV)-infected cell lines secrete lymphokines that can influence the growth and function of a variety of cell types. We recently demonstrated that supernatants from the HTLV-II-infected Mo-T-cell line stimulate the proliferation of rat brain oligodendrocytes and astrocytes. We have now purified a glial growth-promoting factor (GGPF) from these supernatants. Purification from serum-free conditioned medium was accomplished by sequential concentration, ammonium sulfate precipitation, lentillectin affinity chromatography, gel filtration, and reversed-phase high-performance liquid chromatography. GGPF is assayed by its ability to stimulate DNA synthesis in oligodendrocytes, as measured by [3H]thymidine uptake. The purified GGPF has an apparent Mr of 30,000 when analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis under nonreducing conditions. Under reducing conditions, however, GGPF appears as a single band of Mr 18,000. Both reduced and unreduced forms have biological activity, suggesting that GGPF exists in both a functional monomeric and dimeric form. Purified GGPF appears to be a biochemically and functionally distinct lymphokine.
Benveniste
E N
EN
Merrill
J E
JE
Kaufman
S E
SE
Golde
D W
DW
Gasson
J C
JC
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D017977:Deltaretrovirus / Q000032:analysis*; D020904:Glia Maturation Factor; D006133:Growth Substances / Q000302:isolation & purification*; D008213:Lymphocyte Activation; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D009836:Oligodendroglia / Q000187:drug effects; D051381:Rats; D013601:T-Lymphocytes / Q000032:analysis*
D020904:Glia Maturation Factor; D006133:Growth Substances; D009419:Nerve Tissue Proteins