Serum antibodies to the major HSV-2-specified DNA-binding protein in patients with an acute HSV infection or cervical neoplasia.
The major HSV-2-specified DNA-binding protein (ICSP 11/12) was purified from HSV-2-infected cells. ELISA and immunoblotting techniques were used to study its antigenicity in HSV-infected patients and patients with cervical neoplasia and control women. Patients with an acute HSV-2 infection had clearcut antibody responses to the purified ICSP 11/12 preparation. Determination of the ICSP 11/12 antibodies by ELISA revealed considerably higher serum antibody levels in patients with cervical carcinoma than in the controls.
Lehtinen
M
M
Lehtinen
T
T
Koivisto
V
V
Paavonen
J
J
Leinikki
P
P
D000293:Adolescent; D000328:Adult; D000912:Antibodies, Neoplasm / Q000032:analysis; D000914:Antibodies, Viral / Q000276:immunology*; D002277:Carcinoma / Q000032:analysis* / Q000209:etiology / Q000382:microbiology; D002278:Carcinoma in Situ / Q000032:analysis* / Q000209:etiology / Q000382:microbiology; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006558:Herpes Genitalis / Q000150:complications; D006801:Humans; D008297:Male; D008875:Middle Aged; D018139:Simplexvirus / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000032:analysis* / Q000209:etiology / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D000912:Antibodies, Neoplasm; D000914:Antibodies, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; C046299:herpes simplex virus type 2 protein ICSP 11-12