Sequences essential for IS50 transposition. The first base-pair.
Sequences near the ends of the insertion element IS50 are essential for its transposition, probably because they serve as sites upon which the IS50-encoded transposase protein acts. To determine if these essential sequences include the first base-pair at each end of IS50 we generated 5'C to 5'G transversions at these positions. Each mutation reduced the transposition frequency to 1% to 2% of wild-type. DNA sequence analyses showed that the mutant 5'G is preserved during transposition.
Sasakawa
C
C
Phadnis
S H
SH
Carle
G F
GF
Berg
D E
DE
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004279:DNA, Viral; D009154:Mutation; D010957:Plasmids
D004251:DNA Transposable Elements; D004279:DNA, Viral; D004262:DNA Restriction Enzymes