title: Aggravation of experimental cutaneous leishmaniasis in mice by administration of interleukin 3.
abstract: Previous studies from our laboratory have shown that some in vitro maintained Leishmania major-specific L3T4+ T cells were capable of exacerbating cutaneous leishmaniasis after adoptive transfer to normal syngeneic mice. Results presented in this report show that these cells released substantial amounts of interleukin 3 (IL 3) and granulocyte-macrophage colony-stimulating factors after specific stimulation in vitro. In order to assess the involvement of such lymphokines in the exacerbation of cutaneous leishmaniasis by these L3T4+ T cells, the effect of the administration of important doses of IL 3 on the course of infection with L. major was investigated. The treatment of genetically susceptible BALB/c mice with IL 3 resulted in an enhancement of the size of lesions and favored the multiplication of parasites at anatomical sites distant from the primary lesion. Although IL 3 did not modify the development of lesions in genetically resistant CBA mice, this lymphokine promoted the growth of Leishmania in lymph node draining the lesion. Finally, the addition of IL 3 to macrophages parasitized in vitro enhanced the survival of intracellular Leishmania major.
authors.affiliation: WHO-IRTC, University of Lausanne, Epalinges, Switzerland.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Feng
authors.forename: J
authors.initials: J
authors.lastname: Louis
authors.forename: V
authors.initials: V
authors.lastname: Kindler
authors.forename: T
authors.initials: T
authors.lastname: Pedrazzini
authors.forename: J F
authors.initials: JF
authors.lastname: Eliason
authors.forename: R
authors.initials: R
authors.lastname: Behin
authors.forename: P
authors.initials: P
authors.lastname: Vassalli
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D003115:Colony-Stimulating Factors / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000502:physiology*; D007377:Interleukin-3 / Q000494:pharmacology / Q000502:physiology*; D007895:Leishmania tropica / Q000254:growth & development; D007896:Leishmaniasis / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000469:parasitology; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor