Mitogenic stimulation of malignant B cells CLL: diminished in-vitro stimulation with anti-CR1 antibodies.
Peripheral B lymphocytes of five CLL patients were tested in a radioimmunoassay to determine the density of the C3b receptor (CR1) and the cells were assayed for their ability to mature into IgM secreting cells after in-vitro culture with a combination of Pokeweed Mitogen (PWM) and antibodies directed against CR1. Despite the presence of normal amounts of CR1 on the leukemic B cells, crosslinking of these receptors by anti-CR1 antibodies stimulated only a fraction of the leukemic cell population to differentiate into IgM secreting cells. These results add to the partial functional impairment of CLL-B cells.
Bloem
A C
AC
Department of Clinical Immunology, University Hospital, Utrecht, The Netherlands.
Chand
M A
MA
Daha
M R
MR
Bast
B J
BJ
Ballieux
R E
RE
D000906:Antibodies / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000473:pathology*; D008213:Lymphocyte Activation / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011951:Receptors, Complement / Q000276:immunology*; D017463:Receptors, Complement 3b
D000906:Antibodies; D007075:Immunoglobulin M; D011043:Pokeweed Mitogens; D011951:Receptors, Complement; D017463:Receptors, Complement 3b