title: Correlation of rates of calcium entry and endogenous dopamine release in mouse striatal synaptosomes.
abstract: The time course of simultaneous Ca2+ entry and endogenous dopamine release was examined in mouse striatal synaptosomes depolarized by 30 mM KCl. Ca2+ entry and endogenous dopamine release exhibited fast and slow phase processes. The fastest rates occurred between 0 and 1 s. Ca2+ uptake and dopamine release dropped off quickly with 5-15 s rates at 13 and 10%, respectively, of the 0-1 s rate. Both processes were maintained at relatively high rates at the 1-3 and 3-5 s intervals suggesting mixed fast and slow phase processes. Uptake of Ca2+ and release of dopamine occurred in parallel over the entire 30 s measurement period; however, approximately 70% of the Ca2+ uptake and dopamine release occurred within the first 5 s following depolarization. A calculated ratio of Ca2+ entry versus dopamine release showed that approximately 1-2 Ca2+ ions were required to cause the release of one dopamine molecule. This ratio remained constant from 1 to 15 s following depolarization. Our results suggest that Ca2+ entry is coupled to endogenous dopamine release for both the fast and slow phase process.
authors.forename: S W
authors.initials: SW
authors.lastname: Leslie
authors.forename: J J
authors.initials: JJ
authors.lastname: Woodward
authors.forename: R E
authors.initials: RE
authors.lastname: Wilcox
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials; D051379:Mice; D009435:Synaptic Transmission; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D002118:Calcium; D004298:Dopamine