title: Transformation by Epstein-Barr virus requires DNA sequences in the region of BamHI fragments Y and H.
abstract: Eight independent recombinant Epstein-Barr virus genomes, each of which was a transforming strain, were made by superinfecting cell lines containing Epstein-Barr virus DNA (Raji or B95-8 strain) with a nontransforming virus (P3HR1 strain). A knowledge of the constitution of each transforming recombinant allowed the localization of the defect in the genome of the nontransforming parent to a 12-megadalton sequence within the EcoRI A fragment. Within this region, the nontransforming virus has a deletion of the BamHI Y fragment and about half of the sequences in the adjacent BamHI H fragment. The present data suggest that this deletion is responsible for the nontransforming phenotype. Furthermore, mapping a deletion in one of the recombinant genomes allowed the conclusion that a sequence (comprising about 20% of the Epstein-Barr virus genome) from the center of BamHI-D to BamHI-I' is not necessary for the maintenance of transformation by Epstein-Barr virus.
authors.forename: J
authors.initials: J
authors.lastname: Skare
authors.forename: J
authors.initials: J
authors.lastname: Farley
authors.forename: J L
authors.initials: JL
authors.lastname: Strominger
authors.forename: K O
authors.initials: KO
authors.lastname: Fresen
authors.forename: M S
authors.initials: MS
authors.lastname: Cho
authors.forename: H
authors.initials: H
authors.lastname: zur Hausen
mesh_terms: D001483:Base Sequence; D002472:Cell Transformation, Viral; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D010641:Phenotype; D011995:Recombination, Genetic
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI