tRNA binding to programmed ribosomes increases the ribosomal affinity for tuberactinomycin O.
The binding of 14C-labelled tuberactinomycin O was analysed in equilibrium dialysis cells. The ionic conditions and the concentration of the labelled drug used in the binding assays allowed the binding of just one drug molecule per non-programmed ribosome. Under these conditions, the occupation of the ribosomal P-site by deacylated tRNAPhe in the presence of poly(U) increased the amount of [14C]tuberactinomycin O bound by a factor of two. Kanamycin, gentamicin and neomycin reduced the binding of tuberactinomycin O, whereas chloramphenicol, tetracycline, streptomycin and puromycin had no effect. A stimulation of the binding of tuberactinomycin O was found upon addition of erythromycin.
Yamada
T
T
Teshima
T
T
Shiba
T
T
Nierhaus
K H
KH
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002250:Carbon Radioisotopes; D004776:Enviomycin / Q000031:analogs & derivatives / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014508:Urea / Q000378:metabolism; D014756:Viomycin / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; C017784:tuberactinomycin-O; D014508:Urea; D012343:RNA, Transfer; D004776:Enviomycin; D014756:Viomycin