Radioimmunoassay for 21-deoxycortisol: clinical applications.
A radioimmunoassay for 21-deoxycortisol is described. The immunogen, 21-deoxycortisol-3-(0-carboxymethyl) oxime-bovine serum albumin, was prepared, the antisera raised against it were studied and the reliability of the assay was checked. The antiserum selected cross-reacted with 11-deoxycortisol (0.08%), corticosterone (0.25%), cortisol (0.6%) and 17-hydroxyprogesterone (1.6%). 21-deoxycortisol was separated by celite partition chromatography and eluted in the 70/30 (v/v) isooctane/ethyl acetate fraction together with 11-deoxycortisol and corticosterone. The radioimmunoassay was used to measure 21-deoxycortisol in the plasma of normal subjects and patients with androgen excess. In normal subjects, men (0.19 ng/ml +/- 0.08) and women (0.18 ng/ml +/- 0.09) had similar basal levels (mean +/- SD). One hour after ACTH stimulation, these levels were increased by a factor of 3.5. In 7 patients treated for classical congenital adrenal hyperplasia associated with 21-hydroxylase deficiency, basal values varied between 9.1 and 39.9 ng/ml (measured at 8 a.m.). In 7 untreated women with late-onset congenital adrenal hyperplasia (with 21-hydroxylase deficiency), ACTH-stimulated levels were increased to between 9 and 25.5 ng/ml. In 14 heterozygous carriers of 21-hydroxylase deficiency, diagnosed by HLA genotyping, all ACTH-stimulated levels were well above the highest corresponding levels in normal subjects, whereas 17-hydroxyprogesterone levels remained within the normal range in 9 of the cases.
Gueux
B
B
Fiet
J
J
Pham-Huu-Trung
M T
MT
Villette
J M
JM
Gourmelen
M
M
Galons
H
H
Brerault
J L
JL
Vexiau
P
P
Julien
R
R
D015065:17-Hydroxycorticosteroids / Q000097:blood*; D019326:17-alpha-Hydroxyprogesterone; D000312:Adrenal Hyperplasia, Congenital / Q000097:blood; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000328:Adult; D003350:Cortodoxone / Q000097:blood* / Q000276:immunology; D003429:Cross Reactions; D005260:Female; D006241:Haptens; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood; D007106:Immune Sera; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
D015065:17-Hydroxycorticosteroids; D006241:Haptens; D006908:Hydroxyprogesterones; D007106:Immune Sera; C003556:21-deoxycortisol; D019326:17-alpha-Hydroxyprogesterone; D000324:Adrenocorticotropic Hormone; D003350:Cortodoxone