[Gene expression of D-beta-hydroxybutyrate dehydrogenase. III. Molecular cloning of a DNAc].
In order to continue the molecular studies of D-beta-hydroxybutyrate dehydrogenase (BDH) undertaken in our laboratory for several years, we have initiated a genetic approach which consists in the BDH cDNA cloning from a rat liver cDNA library. The immunoscreening method allowed to isolate a clone which exhibits a DNA insert shorter than the expected full length BDH cDNA.
Bailly
A
A
Laboratoire de Biochimie, UA CNRS 531 Biologie Moléculaire des Membranes, Université de Franche-Comté, Besancon, France.
Kanté
A
A
Cherkaoui-Malki
M
M
Adami
P
P
Latruffe
N
N
D000818:Animals; D001345:Autoradiography; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D006884:Hydroxybutyrate Dehydrogenase / Q000235:genetics*; D051381:Rats
D004247:DNA; D006884:Hydroxybutyrate Dehydrogenase
Expression génique de la D-beta-hydroxybutyrate déshydrogénase. III. Clonage moléculaire d'un ADNc.