Characterization of restriction-modification enzymes Cfr13 I from Citrobacter freundii RFL13.
This communicatiopn describes some properties of RCfr13 I and MCfr13 I, isolated from Citrobacter freundii RFL13. RCFfr13 I restriction enzyme recognizes the 5'-G GNCC sequence and cleaves, as indicated by the arrow. MCfr13 I methylase modifies the internal cytosine producing m5C (5'-GGNm5CC). RCfr13 I is sensitive not only to this type of substrate modification but also to hemimethylation in overlapping sites by MCfr10 I (internal cytosine of RCfr13 I recognition is methylated) and MHpa II (external cytosine is methylated). From these results the sensitivity of RCfr13 I to methylation by dcm methylase of E.coli in overlapping sites is deduced.
Bitinaité
J B
JB
Klimasauskas
S J
SJ
Butkus
V V
VV
Janulaitis
A A
AA
D010582:Bacteriophage lambda; D001483:Base Sequence; D002954:Citrobacter / Q000201:enzymology*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D015257:DNA-Cytosine Methylases; D015252:Deoxyribonucleases, Type II Site-Specific; D004789:Enzyme Activation; D008274:Magnesium / Q000494:pharmacology; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D013379:Substrate Specificity
D004279:DNA, Viral; C044884:DNA modification methylase Cfr13I; D015257:DNA-Cytosine Methylases; D008780:Methyltransferases; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C032262:GGNCC-specific type II deoxyribonucleases; D008274:Magnesium