Extension of platelet concentrate storage by addition of sodium bicarbonate.
Viability of platelet concentrate (PC) stored in polyvinylchloride bags in an elliptical rotator at 22 degrees C (standard PC) was assessed by in vitro tests, and an alternate approach to extending the shelf-life of PC by the addition of hypertonic sodium bicarbonate (test PC) was investigated. The fall in the pH which occurred during storage in standard PC was arrested in test PC. Furthermore, platelets stored under these test conditions maintained their morphology better than in standard PC as judged by their mean platelet volume and platelet distribution width. Recovery of stored platelets from hypotonic shock at 37 degrees C following resuspension in fresh plasma was better for test platelets. Results indicated that platelets in standard PC were viable up to day 3 but were not viable at day 7. Platelets store better in PC to which sodium bicarbonate has been added and behave as viable platelets up to 7 days.
Shah
G A
GA
Lenferink
J C
JC
D001639:Bicarbonates / Q000494:pharmacology*; D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007038:Hypotonic Solutions / Q000494:pharmacology; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D010976:Platelet Count; D017693:Sodium Bicarbonate; D013997:Time Factors
D001639:Bicarbonates; D007038:Hypotonic Solutions; D017693:Sodium Bicarbonate; D010100:Oxygen