title: The HLA-AW24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes.
abstract: A cosmid clone containing two class I sequences was found to cause expression of the HLA-AW24 protein after transfection into mouse L cells. The restriction map of this cosmid shows extensive homology over 26 kb with the map of the HLA-A3 region obtained from cosmids of the same library, constructed with DNA from an HLA-A3/HLA-AW24 heterozygote, but diverges over the remaining 14 kb. The HLA-AW24 gene was subcloned from this cosmid and its nucleotide sequence was determined. Amino acid and, more strikingly, nucleotide sequence comparisons with other HLA alleles indicate that the A locus alleles are more closely related to each other than to alleles from other HLA loci. A very skewed distribution of silent substitutions is apparent, and the occurrence of clustered multiple substitutions hints at gene-conversion-like events.
authors.forename: C
authors.initials: C
authors.lastname: N'Guyen
authors.forename: R
authors.initials: R
authors.lastname: Sodoyer
authors.forename: J
authors.initials: J
authors.lastname: Trucy
authors.forename: T
authors.initials: T
authors.lastname: Strachan
authors.forename: B R
authors.initials: BR
authors.lastname: Jordan
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; D059871:HLA-A24 Antigen; D015791:HLA-A3 Antigen; D006801:Humans; D007739:L Cells / Q000276:immunology; D051379:Mice; D014162:Transfection
chemical_list: D006680:HLA Antigens; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; D059871:HLA-A24 Antigen; D015791:HLA-A3 Antigen; D004262:DNA Restriction Enzymes