Inhibition of human platelet aggregation by vitamin K.
The effect of several vitamin K (Vit K) analogues on the aggregation of human platelets was examined. The analogues were potent inhibitors of aggregation induced by ADP, thrombin, collagen and arachidonate but were less active against aggregation induced by the calcium ionophore A23187. Vit K3 also prevented platelet membrane phosphatide breakdown induced by collagen. These effects were not due to a direct inhibition of enzymes involved in the liberation of arachidonate or its subsequent transformation. The analogues exerted no effects on enzymes regulating intraplatelet cAMP. However, these effects could be overcome by increasing extracellular Ca++ levels, indicating a possible interaction with Ca++ regulation in platelets.
Blackwell
G J
GJ
Radomski
M
M
Moncada
S
S
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects*; D013438:Sulfhydryl Compounds / Q000032:analysis; D014812:Vitamin K / Q000494:pharmacology*
D001095:Arachidonic Acids; D010743:Phospholipids; D013438:Sulfhydryl Compounds; D014812:Vitamin K; D016718:Arachidonic Acid; D003094:Collagen; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases