Peripheral-type benzodiazepine receptors in endocrine organs: autoradiographic localization in rat pituitary, adrenal, and testis.
We have used [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, to identify and localize peripheral-type benzodiazepine receptors in endocrine organs. Autoradiographic studies reveal an uniform distribution of [3H]Ro5-4864 binding sites within the anterior, intermediate, and posterior lobes of the pituitary gland, with highest concentrations present in the posterior pituitary. In rat adrenal gland, specific binding sites for [3H]Ro5-4864 are found only in the adrenal cortex, with highest density in the zona glomerulosa and significantly lower concentrations in the zona fasciculata and zona reticularis. [3H]Ro5-4864-associated silver grains in the testis are intensely localized over the interstitial tissue; low concentrations of silver grains are present over the epithelium of the seminiferous tubules but are absent from the tubular lumen. These studies demonstrate a differential and discrete localization of peripheral-type benzodiazepine receptors in rat pituitary, adrenal, and testis.
De Souza
E B
EB
Anholt
R R
RR
Murphy
K M
KM
Snyder
S H
SH
Kuhar
M J
MJ
D000311:Adrenal Glands / Q000032:analysis*; D000818:Animals; D001345:Autoradiography; D001570:Benzodiazepinones / Q000378:metabolism; D001667:Binding, Competitive; D002998:Clonazepam / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D008297:Male; D010902:Pituitary Gland / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000032:analysis*; D013737:Testis / Q000032:analysis; D014018:Tissue Distribution
D001570:Benzodiazepinones; D007546:Isoquinolines; D011963:Receptors, GABA-A; C028513:4'-chlorodiazepam; D002998:Clonazepam; C037850:PK 11195