Two pathways for oxygen exchange by heavy meromyosin and their dependence on actin.
In the hydrolysis of MgATP by acto heavy meromyosin (HMM) there are two enzymatic pathways that differ in the properties of their intermediate oxygen exchange; one of these is designated the low exchange pathway (P1); the other is designated the high exchange pathway (P2). A plot of the P1 flux versus the actin concentration gives a sigmoid curve, whereas the corresponding curve for the P2 flux rises in an approximately hyperbolic manner. At low concentrations of actin, where the sigmoid curve of the P1 flux is in a lag phase, the major flux is along P2; but at higher concentrations of actin, as the P1 curve rises sharply, the flux along P1 comes to predominate. Even at the highest levels of actin, at saturating levels for both pathways, the kinetics of exchange along P1 and P2 are significantly different. In addition to these differences in the actin dependence, the flux of P1 relative to P2 is markedly inhibited by KCl. Therefore, which of the two pathways dominates during the hydrolysis of MgATP by HMM is strongly dependent on experimental conditions. The findings suggest that P1 involves the interaction of HMM with two actin units whereas P2 involves the interaction of HMM with one actin unit. The results are discussed in relation to a kinetic scheme based on this proposal.
Shukla
K K
KK
Department of Physiology and Biophysics, School of Medicine, State University of New York, Stony Brook 11794.
Levy
H M
HM
Ramirez
F
F
Marecek
J F
JF
D000199:Actins / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism; D015879:Myosin Subfragments / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D011189:Potassium Chloride / Q000378:metabolism; D011817:Rabbits; D014357:Trypsin / Q000378:metabolism
D000199:Actins; D015879:Myosin Subfragments; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D002918:Chymotrypsin; D014357:Trypsin; D000251:Adenosine Triphosphatases; D010100:Oxygen