"Cytoplasmic" islet cell antibodies. Evidence that the target antigen is a sialoglycoconjugate.
We have biochemically treated (periodate, borohydride, neuraminidase, organic solvents) frozen sections of human pancreas and studied the reactivity of islet-cell-antibody-positive human sera and monoclonal antibodies. The autoantigen of pancreatic sections has the properties of sialic acid containing glycolipid.
Nayak
R C
RC
Omar
M A
MA
Rabizadeh
A
A
Srikanta
S
S
Eisenbarth
G S
GS
D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D003593:Cytoplasm / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007515:Islets of Langerhans / Q000276:immunology*; D009439:Neuraminidase / Q000378:metabolism; D010504:Periodic Acid; D011402:Pronase / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D006017:Glycolipids; D012794:Sialic Acids; D010504:Periodic Acid; C009288:metaperiodate; D009439:Neuraminidase; D011402:Pronase