Allogeneic lymphocyte stimulation by human spleen. Identification of cells that are very effective for presenting class II HLA antigens.
Human spleen cells were fractionated by percoll density gradient centrifugation and by sorting in the FACS with mixtures of fluorescent antibodies against T cells, B cells, monocytes, and Sig-bearing cells. Cells responsible for powerful MLR stimulation were class II HLA antigen-positive and were concentrated in preparations depleted of all the markers listed above. These cells represented 1-2% of the initial spleen cells. They were remarkably more active than other HLA class II antigen-positive cells. The procedure described allows rapid enrichment for the responsible cells. It should be useful for further characterization of these cells and for performing studies on their function.
Pettaway
C A
CA
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
Ball
E J
EJ
Stastny
P
P
D000938:Antigen-Presenting Cells / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D002448:Cell Adhesion; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology
D000944:Antigens, Differentiation, B-Lymphocyte; D006684:HLA-DR Antigens; D011947:Receptors, Antigen, B-Cell