Interaction of prostaglandin D2 with prostacyclin, carbacyclin and the hydantoin prostaglandin, BW245C, in guinea-pig platelets.
The anti-aggregating actions of prostaglandin D2 (PGD2) have been compared to prostacyclin (PGI2), its stable analogue carbacyclin and a hydantoin prostaglandin, BW245C, in guinea-pig PGI2, carbacyclin and BW245C were potent inhibitors of ADP-induced aggregation in guinea-pig platelets, with ID50 values comparable to those obtained in human platelet-rich-plasma. In contrast, PGD2 acted as a weak and partial inhibitor in guinea-pig platelet aggregation, producing a bell-shaped dose-response relationship. PGD2 induced a dose-related antagonism of the inhibitory actions of BW245C, prostacyclin and carbacyclin on guinea-pig platelets. However, PGD2 did not antagonize the inhibitory actions of either forskolin or dibutyryl cyclic AMP on this platelet preparation. The results suggest a non-specific interaction of PGD2 with these prostanoid binding sites on guinea-pig platelets.
Hamid
S
S
Whittle
B J
BJ
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D003994:Bucladesine / Q000494:pharmacology; D005576:Colforsin; D004224:Diterpenes / Q000037:antagonists & inhibitors; D004347:Drug Interactions; D011464:Epoprostenol / Q000494:pharmacology* / Q000031:analogs & derivatives; D006168:Guinea Pigs; D006827:Hydantoins / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D015230:Prostaglandin D2; D011457:Prostaglandins D / Q000494:pharmacology*
D004224:Diterpenes; D006827:Hydantoins; D011457:Prostaglandins D; D005576:Colforsin; D000244:Adenosine Diphosphate; D003994:Bucladesine; C026678:carboprostacyclin; C030669:BW 245C; D011464:Epoprostenol; D015230:Prostaglandin D2