title: Immunoelectrophoretic characterization of the ADP/ATP carrier from heart, kidney, and liver.
abstract: Earlier studies gave an indication for an organ specificity of the ADP/ATP carrier. We used a modified charge-shift crossed immunoelectrophoresis for a more precise immunochemical characterization of this detergent-solubilized hydrophobic membrane protein. Immunological differences between the carrier protein from heart, kidney, and liver were demonstrated by a different electrophoretic migration of the three ligand-protein complexes in the first dimension and a distinct staining intensity, sharpness, and shape of the precipitates in the second dimension. However, the antibodies against the heart and kidney protein showed a cross-reactivity between the three antigens. The results are consistent with the view that the ADP/ATP carrier has organ-specific antigenic determinants although there is a partial identity between the carrier proteins from heart, kidney, and liver.
authors.forename: H P
authors.initials: HP
authors.lastname: Schultheiss
authors.forename: M
authors.initials: M
authors.lastname: Klingenberg
mesh_terms: D000818:Animals; D002417:Cattle; D003902:Detergents; D007123:Immunoelectrophoresis, Two-Dimensional; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D000226:Mitochondrial ADP, ATP Translocases / Q000032:analysis*; D009206:Myocardium / Q000201:enzymology*; D009713:Nucleotidyltransferases / Q000032:analysis*; D009928:Organ Specificity; D011487:Protein Conformation; D012995:Solubility
chemical_list: D003902:Detergents; D000226:Mitochondrial ADP, ATP Translocases; D009713:Nucleotidyltransferases