Detection of deoxyribonucleoside-triphosphate imbalance death-induced DNA double strand breakage in FM3A cells by orthogonal-field-alternation gel electrophoresis (OFAGE).
The mechanism of intracellular deoxyribonucleoside-triphosphate (dNTP) imbalance death of mouse mammary tumor FM3A cells was studied. When the cells were exposed to 5-fluorodeoxyuridine, deoxyadenosine, or 2-chlorodeoxyadenosine, dNTP pool imbalance resulted. The imbalance was followed by DNA double strand breaks and subsequent cell death. The DNA double strand breaks have been directly examined by means of orthogonal-field-alternation gel electrophoresis (OFAGE). Fragmented DNA band appeared to be approximately 100-200 kb in size.
Wataya
Y
Y
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Hazawa
T
T
Watanabe
K
K
Hirota
Y
Y
Yoshioka-Hiramoto
A
A
D000818:Animals; D002460:Cell Line; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects; D003854:Deoxyribonucleotides / Q000378:metabolism*; D005467:Floxuridine / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D051379:Mice
D004273:DNA, Neoplasm; D003854:Deoxyribonucleotides; D005467:Floxuridine