Monoclonal anti-idiotypic antibodies to an I-J interacting molecule inhibit suppression in an H-2 restricted way.
We have obtained 10 mAb from two independent fusions that are anti-idiotypic to the combining site of an anti-I-Jk antibody. These mAb block Ts cell function isn a genetically restricted manner in vitro and in vivo and recognize a determinant on macrophage membranes. In addition, they do not affect the I-Ek-restricted activation of a Th cell line specific for pigeon heart cytochrome c. We conclude that these mAb may recognize a molecule other than conventional I-Ek on cells interacting with Ts cells that is involved in mediating Ts activity. The molecule recognized may be a modified I-Ek molecule or a molecule not encoded by the genes encoding I-Ek.
Jaureguiberry
B
B
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.
Liao
L
L
Kuchroo
V
V
Dorf
M E
ME
Diamond
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000502:physiology*; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D008213:Lymphocyte Activation; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010455:Peptides / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000037:antagonists & inhibitors; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; C043911:I-J-antigen; D007130:Immunoglobulin Idiotypes; D007166:Immunosuppressive Agents; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D013491:Suppressor Factors, Immunologic; C027606:L-glutamic acid-L-tyrosine copolymer