Carbon nuclear magnetic resonance spectra of oligosaccharides isolated from human milk and ovarian cyst mucin.
Natural abundance Carbon-13 nuclear magnetic resonance spectra at 20 MHz were reported for the three common human milk oligosaccharides, lacto-N-tetraose and lacto-N-fucopentaoses I and II, as well as for two related tetra- and hexasaccharide alditols isolated from the alkaline borohydride degradation products of an ovarian cyst glycoprotein. Spectral assignments made with the help of deuterium-induced shift (DIS), attached proton test (APT), and T1 data indicated some very irregular glycosylation shifts which were attributed to effects of steric crowding and non-nearest-neighbor interactions. Samples as small as 10 mumol of oligosaccharide gave acceptable 20-MHz spectra with the use of a 5-mm probe coil.
Bush
C A
CA
Panitch
M M
MM
Dua
V K
VK
Rohr
T E
TE
D002247:Carbon Isotopes; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008895:Milk, Human / Q000032:analysis*; D009077:Mucins / Q000032:analysis*; D009844:Oligosaccharides / Q000302:isolation & purification*; D010048:Ovarian Cysts / Q000032:analysis*; D011522:Protons
D002247:Carbon Isotopes; D009077:Mucins; D009844:Oligosaccharides; D011522:Protons; C041762:lacto-N-fucopentaose II; C045442:lacto-N-fucopentaose I; C013084:lacto-N-neotetraose