DNA-binding proteins present in varicella-zoster virus-infected cells.
DNA-binding proteins present in varicella-zoster virus-infected cells were identified by DNA-cellulose chromatography of radioactively labeled cell extracts. Seven virus-specific proteins, ranging in molecular weight from approximately 175,000 to 21,000, showed affinity for single- or double-stranded DNA or both. These proteins include the varicella-zoster virus major capsid protein, a phosphorylated tegument protein, and a 125,000-molecular-weight species which may be analogous to the major DNA-binding protein of herpes simplex virus. We also identified a number of DNA-binding phosphoproteins by these procedures. Finally, protein blot studies were carried out to determine whether these proteins bind preferentially to virus rather than to host cell DNA.
Roberts
C R
CR
Weir
A C
AC
Hay
J
J
Straus
S E
SE
Ruyechan
W T
WT
D001665:Binding Sites; D002213:Capsid / Q000378:metabolism; D002644:Chickenpox / Q000235:genetics* / Q000378:metabolism; D002846:Chromatography, Affinity; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D014645:Herpesvirus 3, Human; D008970:Molecular Weight; D010750:Phosphoproteins / Q000302:isolation & purification* / Q000378:metabolism; D014764:Viral Proteins / Q000302:isolation & purification* / Q000378:metabolism
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D014764:Viral Proteins