title: Ultrahistochemical localization of adenylate cyclase activity in the electric organ of Torpedo marmorata.
abstract: The lead pyrophosphate precipitation technique was used to visualize adenylate cyclase activity with the electron microscope in unfixed electric organ and synaptosomes of Torpedo marmorata, with special attention to presynaptic membranes. Specificity of the deposition of reaction product was ensured by using 5'-adenylyl imidodiphosphate as substrate and 5'-guanylyl imidodiphosphate and sodium fluoride as activators. Under suitable conditions a reaction product was deposited on the Schwann cell, on presynaptic vesicles, on the inner side of membranes of cisternae and on glycogen granules of the presynaptic region of the endplate. In some cases, a precipitate was also found on postsynaptic membranes of the synaptic cleft and on mitochondria. In isolated synaptosomes localization of the reaction product was identical with that of minced tissue. However, most strikingly, on presynaptic membranes no precipitate was ever found, neither in pieces of electric organ nor in isolated synaptosomes. Furthermore, the extended membrane system of the postsynaptic region of the electroplax remained always free of lead pyrophosphate precipitate.
authors.forename: U
authors.initials: U
authors.lastname: Müller
authors.forename: K
authors.initials: K
authors.lastname: Munz
authors.forename: P G
authors.initials: PG
authors.lastname: Waser
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis; D004557:Electric Organ / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D006651:Histocytochemistry; D008854:Microscopy, Electron; D014101:Torpedo
chemical_list: D000242:Cyclic AMP; D000262:Adenylyl Cyclases