title: Positive regulation of the colicin E1 gene by cyclic AMP and cyclic AMP receptor protein.
abstract: In previous experiments, we showed that the in vivo transcription of the colicin E1 gene was dependent on cyclic AMP in adenylate cyclase-defective mutant cells of Escherichia coli (Ebina, Y. and Nakazawa, A (1983) J. Biol. Chem. 258, 7072-7078). We now show that cyclic AMP and cyclic AMP receptor protein stimulated the in vitro transcription of the gene in the presence of spermidine. As determined in DNase I protection experiments, two binding sites for the complex of cyclic AMP and the receptor protein were identified about 60 base pairs (CRP-1) and 110 base pairs (CRP-2) upstream from the transcription initiation site of the colicin E1 gene. CRP-1 had a higher affinity for the complex than that of CRP-2. Substituting an unrelated DNA sequence for CRP-2 reduced the efficiency of in vitro stimulation of the gene by cyclic AMP and the receptor protein. These potential binding sites for the cyclic AMP-cyclic AMP receptor protein complex probably participate in the stimulation of the colicin E1 gene transcription.
authors.forename: K
authors.initials: K
authors.lastname: Shirabe
authors.forename: Y
authors.initials: Y
authors.lastname: Ebina
authors.forename: T
authors.initials: T
authors.lastname: Miki
authors.forename: T
authors.initials: T
authors.lastname: Nakazawa
authors.forename: A
authors.initials: A
authors.lastname: Nakazawa
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003087:Colicins / Q000235:genetics*; D000242:Cyclic AMP / Q000502:physiology*; D012321:DNA-Directed RNA Polymerases; D005786:Gene Expression Regulation / Q000187:drug effects; D009876:Operon; D011189:Potassium Chloride / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D011953:Receptors, Cyclic AMP / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D003087:Colicins; D012333:RNA, Messenger; D011953:Receptors, Cyclic AMP; D011189:Potassium Chloride; D000242:Cyclic AMP; D012321:DNA-Directed RNA Polymerases