A direct radioimmunoassay for the determination of adrenocorticotropic hormone (ACTH) and a clinical evaluation.
A direct radioimmunoassay for the determination of ACTH in a small volume of serum was developed using a commercially available antiserum and tracer. A single-stage radioimmunoassay with an incubation period of 4 days gave reliable and reproducible ACTH values. In the incubation medium 0.1% 2-mercaptoethanol was used as the antioxidant. During a 15-month period the assay has been used routinely in the laboratory. The coefficient of variation was less than 10% in the physiological and pathological range. A normal range of 12 to 60 ng/L was found. The reliability of the assay is demonstrated by results obtained in patients with disorders of the pituitary-adrenal axis.
Arts
C J
CJ
Koppeschaar
H P
HP
Veeman
W
W
Thijssen
J H
JH
D000324:Adrenocorticotropic Hormone / Q000097:blood*; D002940:Circadian Rhythm; D003480:Cushing Syndrome / Q000097:blood; D005069:Evaluation Studies as Topic; D006801:Humans; D008623:Mercaptoethanol; D011786:Quality Control; D011863:Radioimmunoassay / Q000379:methods*; D012016:Reference Values
D008623:Mercaptoethanol; D000324:Adrenocorticotropic Hormone