Binding of immunoglobulin G to peripheral blood lymphocytes.
The specific and saturable binding of FITC conjugate of aggregated goat IgG to goat peripheral blood lymphocytes was studied in PBS containing 1% BSA. The polar nature of the specific interaction of heterologous aggregated IgG, IgG monomer and its fragment F(ab'2) with the cells was studied by ELISA using the peroxidase conjugated F(ab'2) of anti-human IgG under different conditions of pH and ionic strength.
Hajela
K
K
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, India.
Salahuddin
A
A
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001665:Binding Sites; D006041:Goats; D006863:Hydrogen-Ion Concentration; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G / Q000378:metabolism*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D009994:Osmolar Concentration; D011961:Receptors, Fc / Q000378:metabolism; D017452:Receptors, IgG
D000943:Antigens, Differentiation; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG