[A plasmid vector with temperature-controlled gene expression].
A 169 b.p. fragment including the bla gene promoter p3 has been removed from pBR327 plasmid, and the deleted plasmid used for cloning the TaqI/BglII-fragment of the lambda c1857ind- DNA containing promoter pR and gene cI to obtain plasmid pCE119. Cells containing pCE119 produced a high level of beta-lactamase at 42 degrees C, the yield at 42 degrees C being 100 times higher than at 32 degrees C. For cloning and functional assays a pCEZ12 plasmid was constructed, in which promoter pR and repressor cI of lambda phage control the expression of the semi-synthetic beta-galactosidase gene. Yield of beta-galactosidase produced by pCEZ12 at 42 degrees C was ca. 300 times higher than at 32 degrees C.
Kravchenko
V V
VV
Iamshchikov
V F
VF
Pletnev
A G
AG
D010582:Bacteriophage lambda / Q000235:genetics; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D005822:Genetic Vectors; D007763:Lac Operon; D010957:Plasmids; D013696:Temperature; D001616:beta-Galactosidase / Q000235:genetics
D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase
Plazmidnyĭ vektor s kontroliruemoĭ temperaturoĭ ékspressieĭ genov