[Na, K-ATPase activity of the microsomal fraction of the rat brain exposed to insulin].
The effect of insulin on the activity of Na, K-ATPase was studied in rat brain microsomes. Addition of insulin to the incubation medium in a dose of 0.18 U/ml coupled with strophanthine did not change the enzyme activity. The raising of the hormone dose to 0.36 U/ml was accompanied by inhibition of the enzyme activity. The incubation duration (10 and 30 min) did not influence the Na-pump. Preincubation of brain microsomes with insulin for 5 min significantly activated Na, K-ATPase. It has been thus demonstrated that insulin is capable of influencing the activity of Na, K-ATPase of rat brain microsomes in vitro. The effect obtained depends both on the dose of the hormone introduced into the incubation medium and the experimental conditions.
Komissarenko
V P
VP
Efimova
E K
EK
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D008861:Microsomes / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013997:Time Factors
D007328:Insulin; D000254:Sodium-Potassium-Exchanging ATPase
Aktivnost' Na, K-ATFazy mikrosomnoĭ fraktsii golovnogo mozga krys pri deĭstvii insulina.