Enzymatic detection of phospholipid biosynthetic enzymes after electroblotting.
The membrane-associated enzymes phosphatidylinositol synthase (CDPdiacylglycerol:myo-inositol 3-phosphatidyltransferase; EC 2.7.8.11) and phosphatidylserine synthase (CDPdiacylglycerol:L-serine O-phosphatidyltransferase; EC 2.7.8.8) from Saccharomyces cerevisiae were detected enzymatically after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotting. Enzyme activities were measured on nitrocellulose blots by using pure enzyme preparations as well as Triton X-100-solubilized membranes. Phosphatidylinositol synthase activity migrated to Mr 34,000, and phosphatidylserine synthase activity migrated to Mr 23,000.
Poole
M A
MA
Fischl
A S
AS
Carman
G M
GM
D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D010770:Phosphotransferases / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D017855:Transferases (Other Substituted Phosphate Groups)
D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase