Isolation and characterisation of a cDNA clone for human apolipoprotein CI and assignment of the gene to chromosome 19.
We have synthesised a mixed oligonucleotide 17 bases long and used it to isolate cDNA clones for apolipoprotein CI (apo CI) from an adult liver cDNA library. The partial sequence of one of these clones confirms its identity. We have used this probe and Southern blotting techniques to identify the human apo CI gene in DNA from a series of rodent X human somatic cell hybrids. Our results provide evidence for the assignment of this gene to human chromosome 19.
Tata
F
F
Henry
I
I
Markham
A F
AF
Wallis
S C
SC
Weil
D
D
Grzeschik
K H
KH
Junien
C
C
Williamson
R
R
Humphries
S E
SE
D000818:Animals; D053302:Apolipoprotein C-I; D001056:Apolipoproteins C / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002903:Chromosomes, Human, 19-20; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009841:Oligonucleotides
D053302:Apolipoprotein C-I; D001056:Apolipoproteins C; D004274:DNA, Recombinant; D009841:Oligonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes