title: An immunocytochemical method for assaying oestrogen receptors in breast cancers. A comparison with the steroid binding assay.
abstract: The presence of oestrogen receptors was studied in 105 human breast carcinomas using monoclonal antibodies (Abbott ER-ICA kit). The oestrogen receptors of neoplastic cells were semiquantitatively measured and correlations were made to receptor values determined by a dextran-coated charcoal (DCC) steroid binding assay and to histological grade. Immunoreactive cells were found in about 2/3 of the tumours. Usually only a fraction of the cells within each tumour were immunoreactive, and the staining intensity varied among different cells. In general, well differentiated tumours had a greater proportion of immunoreactive cells than poorly differentiated ones. In most cases (65/98) a good agreement was found between the ER-ICA method and the DCC assay. However, in 33 cases discrepancies were demonstrated.
authors.affiliation: Department of Biochemistry, Norwegian Radium Hospital, Oslo.
authors.forename: L O
authors.initials: LO
authors.lastname: Ottestad
authors.forename: K M
authors.initials: KM
authors.lastname: Tveit
authors.forename: J M
authors.initials: JM
authors.lastname: Nesland
authors.forename: I
authors.initials: I
authors.lastname: Dybsjord
authors.forename: O
authors.initials: O
authors.lastname: Børmer
authors.forename: J
authors.initials: J
authors.lastname: Høie
authors.forename: A
authors.initials: A
authors.lastname: Pihl
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D011960:Receptors, Estrogen / Q000032:analysis*; D013256:Steroids / Q000378:metabolism
chemical_list: D011960:Receptors, Estrogen; D013256:Steroids