A threshold effect in the induction of tumorigenicity of an established human cell line by v-mos.
The nontumorigenic immortal human cell line, SV80, was transfected with the v-mos gene to assess the gene's effect on tumorigenicity of cultured human cells. Two classes of cells, each containing functional v-mos, were obtained. The first class contained low levels of v-mos RNA, was morphologically transformed, but was nontumorigenic in nude mice. The second was also morphologically transformed, but contained high levels of v-mos RNA and was tumorigenic. The results indicate that SV80 cells behave similarly to murine fibroblasts in their response to v-mos in that they can be rendered tumorigenic by the viral oncogene. However, tumorigenicity was effected through a mechanism which involves different threshold doses for morphologic and tumorigenic transformation.
O'Hara
B M
BM
Laboratory of Molecular Oncology, NCI-FCRF, Frederick, Maryland 21701-1013.
Blair
D G
DG
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000209:etiology / Q000473:pathology*; D002472:Cell Transformation, Viral; D005347:Fibroblasts / Q000473:pathology / Q000637:transplantation; D006367:HeLa Cells / Q000473:pathology / Q000637:transplantation; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008980:Moloney murine sarcoma virus / Q000235:genetics; D009374:Neoplasms, Experimental / Q000209:etiology*; D017232:Oncogene Proteins v-mos; D012191:Retroviridae Proteins / Q000502:physiology*
D000952:Antigens, Polyomavirus Transforming; D017232:Oncogene Proteins v-mos; D012191:Retroviridae Proteins