Regulation of expression of the crp gene of Escherichia coli K-12: in vivo study.
Expression of the crp gene was studied in vivo by use of a crp-lacZ gene fusion first constructed on a plasmid and then transferred onto the chromosome. Our in vivo data confirm the in vitro findings that crp is negatively autoregulated via the cyclic AMP-catabolite gene activator protein complex. We present evidence that gene crp is repressed by glucose.
Cossart
P
P
Gicquel-Sanzey
B
B
D000242:Cyclic AMP / Q000494:pharmacology; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005798:Genes, Bacterial; D005947:Glucose / Q000494:pharmacology; D007763:Lac Operon; D010957:Plasmids; D011953:Receptors, Cyclic AMP / Q000235:genetics*; D014158:Transcription, Genetic; D014161:Transduction, Genetic
D004274:DNA, Recombinant; D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP; D005947:Glucose