[Comparative study of the kinetic properties of the neutral maltase of human granulocytes and of the kidney maltase of the rat].
Neutral maltase from human granulocytes has a different substrate specificity from the human neutral maltase of kidney, though it has been reported that these two enzymes are immunologically similar. We report here that human granulocyte neutral maltase is similar to the neutral maltase from rat's kidney as regards the substrate specificity and the inhibition by Tris and maltodextrins. We also report a different thermal stability that might imply some structural differences between the two enzymes.
Stio
M
M
Département des Sciences biochimiques, Université de Florence, Italie.
Vanni
P
P
Pinzauti
G
G
D000818:Animals; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D008297:Male; D011134:Polysaccharides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors; D014325:Tromethamine / Q000494:pharmacology; D000520:alpha-Glucosidases / Q000493:pharmacokinetics*
D011134:Polysaccharides; D014325:Tromethamine; C008315:maltodextrin; D000520:alpha-Glucosidases
Etude comparée des propriétés cinétiques de la maltase neutre des granulocytes humains et de la maltase rénale du rat.