title: Specific substrate for histone kinase II: a synthetic nonapeptide.
abstract: Based on the previously determined intrinsic substrate specificity of histone kinase II, a nonapeptide was synthesized which was a specific substrate for this enzyme. The Vmax value of phosphorylation of the peptide (Ala-Ala-Ala-Ser-Phe-Lys-Ala-Lys-Lys-amide) was about the same as that for H1 histone and the apparent Km for the phosphorylation of the peptide was 0.2 mM, an order of magnitude higher than that for H1 histone. H1 histone inhibited the phosphorylation of the peptide, while the peptide did not inhibit the phosphorylation of H1 histone. In the crude extracts of calf thymus, spleen and liver, histone kinase II was the only enzyme which phosphorylated the synthetic peptide. The rate of phosphorylation of this peptide was used to determine the activity of histone kinase II in the crude extracts of several tissues obtained from different species.
authors.forename: T
authors.initials: T
authors.lastname: Romhányi
authors.forename: J
authors.initials: J
authors.lastname: Seprödi
authors.forename: F
authors.initials: F
authors.lastname: Antoni
authors.forename: G
authors.initials: G
authors.lastname: Mészáros
authors.forename: A
authors.initials: A
authors.lastname: Faragó
mesh_terms: D000818:Animals; D006168:Guinea Pigs; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009842:Oligopeptides / Q000378:metabolism; D010766:Phosphorylation; D006656:Protamine Kinase / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013154:Spleen / Q000201:enzymology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013950:Thymus Gland / Q000201:enzymology
chemical_list: D009842:Oligopeptides; D011494:Protein Kinases; D006656:Protamine Kinase