Possible applications of recombinant DNA technology to the diagnosis and study of variants of alpha 1-antitrypsin.
We describe the basic principles of recombinant DNA technology and how this methodology has been used to isolate and characterise cloned genes coding for alpha 1-antitrypsin. We show how these probes can be used for diagnosis and to study molecular variants of alpha 1-antitrypsin which may predispose individuals to develop lung disease.
Kalsheker
N
N
Chiswell
D
D
Markham
A
A
Imam
A
A
Wallis
S
S
Williamson
R
R
Humphries
S E
SE
D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005819:Genetic Markers; D006801:Humans; D008171:Lung Diseases / Q000201:enzymology / Q000235:genetics; D009154:Mutation; D012306:Risk; D012907:Smoking; D000515:alpha 1-Antitrypsin / Q000235:genetics*
D000596:Amino Acids; D004274:DNA, Recombinant; D005819:Genetic Markers; D000515:alpha 1-Antitrypsin; D004247:DNA; D004262:DNA Restriction Enzymes