Posttransfusion non-A, non-B hepatitis in chimpanzees. Physicochemical evidence that the tubule-forming agent is a small, enveloped virus.
Posttransfusion non-A, non-B hepatitis associated with the formation of hepatocyte cytoplasmic tubules was experimentally transmitted to chimpanzees by intravenous inoculation of a proven-infectious plasma that had been pelleted and microfiltrated, or purified by a combination of pelleting and rate-zonal banding. The results of these studies indicate that a factor VIII-derived non-A, non-B tubule-forming agent will pass through an 80-nm membrane filter and that it can be recovered from infected plasma by use of a purification procedure that assumes the non-A, non-B tubule-forming agent is a small, enveloped virus. Our findings, in combination with the known sensitivity of the non-A, non-B tubule-forming agent to chloroform and its apparent lack of nucleic acid homology with hepatitis B virus, further suggest that at least one etiologic agent of human posttransfusion non-A, non-B hepatitis may be a small, enveloped RNA virus.
Bradley
D W
DW
McCaustland
K A
KA
Cook
E H
EH
Schable
C A
CA
Ebert
J W
JW
Maynard
J E
JE
D000818:Animals; D002725:Chloroform / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006526:Hepatitis C / Q000209:etiology / Q000382:microbiology*; D006525:Hepatitis, Viral, Human / Q000382:microbiology*; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008870:Microtubules / Q000648:ultrastructure; D002679:Pan troglodytes; D012328:RNA Viruses / Q000187:drug effects / Q000648:ultrastructure; D018108:Respirovirus / Q000648:ultrastructure; D065227:Transfusion Reaction
D002725:Chloroform