A method for detecting human enteroviruses in aquatic sediments.
A method is described for detecting enteroviruses in both freshwater and marine sediments. Viruses were recovered from sediments by elution into 6% beef extract at pH 9.0 and concentration with polyethylene glycol 6000. The recovery efficiency ranged from 6 to 55% for marine sediments and 16 to 77% for freshwater sediments. Although the efficiency of the method was influenced by the composition and source of the sediments it was used successfully to detect viruses occurring in marine and freshwater sediments near sewer outfalls.
Lewis
G D
GD
Loutit
M W
MW
Austin
F J
FJ
D004770:Enterovirus / Q000302:isolation & purification*; D005618:Fresh Water; D012623:Seawater; D012722:Sewage / Q000032:analysis; D012988:Soil Microbiology
D012722:Sewage