Anti-CD3 antibodies induce T helper function for human B cell differentiation in vitro by an interleukin 2-independent pathway.
Previously we have shown that interleukin 2 (IL 2) is an essential mediator in T cell-dependent B cell differentiation induced by pokeweed mitogen. Here we show that activation with monoclonal anti-CD3 antibodies of peripheral blood T cells led to the induction of helper activity for IgM secretion by human B cells from a prolymphatic leukemia. With the use of monoclonal antibodies against the IL 2 receptor and CD3+CD4+CD8- chronic lymphatic leukemia T cells with a strongly reduced capacity to produce IL 2, it was demonstrated that the anti-CD3-driven Ig secretion was obtained by an IL 2-independent pathway. The T cell help in this system is mediated by soluble factors.
Sauerwein
R W
RW
Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Van der Meer
W G
WG
Van Oostveen
J W
JW
Miedema
F
F
Aarden
L A
LA
D000911:Antibodies, Monoclonal / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D007376:Interleukin-2 / Q000502:physiology*; D007945:Leukemia, Lymphoid / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D011043:Pokeweed Mitogens / Q000494:pharmacology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D007075:Immunoglobulin M; D007376:Interleukin-2; D011043:Pokeweed Mitogens