Adenine nucleotides directly stimulate pertussis toxin.
Both cholera toxin and pertussis toxin catalyzed ADP-ribosylation of purified bovine brain tubulin. The effect of cholera toxin was evident in the absence or presence of nucleotides. In contrast, pertussis toxin required adenine nucleotides for its ADP-ribosylating activity. ATP, ATP gamma S, App(NH)p, deoxy-ATP, and ADP all supported pertussis toxin-catalyzed ADP-ribosylations in the absence or presence of EDTA, suggesting that nucleotide hydrolysis was not involved. Adenine nucleotides also promoted pertussis toxin-catalyzed ADP-ribosylation of heat-treated bovine serum albumin. This result suggests that adenine nucleotides directly affect pertussis toxin. ATP stimulation of pertussis toxin-catalyzed hydrolysis of NAD to ADP-ribose supports this hypothesis.
Lim
L K
LK
Sekura
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Kaslow
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D000227:Adenine Nucleotides / Q000494:pharmacology*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000266:Adenylyl Imidodiphosphate / Q000494:pharmacology; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D001923:Brain Chemistry; D002417:Cattle; D002772:Cholera Toxin / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000378:metabolism; D037342:Pertussis Toxin; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D010566:Virulence Factors, Bordetella
D000227:Adenine Nucleotides; D001427:Bacterial Toxins; D014404:Tubulin; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D000266:Adenylyl Imidodiphosphate; D012710:Serum Albumin, Bovine; D002772:Cholera Toxin; D037342:Pertussis Toxin; D009244:NAD+ Nucleosidase