title: Sialosyllactotetraosylceramide, a novel ganglioside antigen detected in human carcinomas by a monoclonal antibody.
abstract: A novel ganglioside was detected in a small cell lung carcinoma by TLC-immunostaining of gangliosides with a monoclonal antibody, the C-50 MAb. Structural characterization showed this ganglioside to be IV3NeuAc-LcOse4Cer, a hitherto unknown ganglioside. This ganglioside has also been detected as a minor component in many different carcinomas using the C-50 MAb. The normally dominant CA-50 ganglioside antigen is IV3NeuAc, III4Fuc-LcOse4Cer. Based upon solid-phase binding to IV3NeuAc, III4-LcOse4Cer and IV3NeuAc-LcOse4Cer it is concluded that the C-50 MAb recognizes an epitope present in sialylated type I carbohydrate chains.
authors.forename: O
authors.initials: O
authors.lastname: Nilsson
authors.forename: J E
authors.initials: JE
authors.lastname: Månsson
authors.forename: L
authors.initials: L
authors.lastname: Lindholm
authors.forename: J
authors.initials: J
authors.lastname: Holmgren
authors.forename: L
authors.initials: L
authors.lastname: Svennerholm
mesh_terms: D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D018288:Carcinoma, Small Cell / Q000032:analysis*; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis* / Q000276:immunology; D005915:Globosides; D006801:Humans; D007158:Immunologic Techniques; D008175:Lung Neoplasms / Q000032:analysis*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D005732:Gangliosides; D005915:Globosides; C037790:sialosylgloboside