A combined enzyme immunoassay for rotavirus and adenovirus (EIARA).
A combined enzyme immunoassay for rotavirus and adenovirus (EIARA) was developed as a double-antibody sandwich assay in which test samples are added to plastic wells coated with rotavirus or adenovirus goat antibody. The presence of antigens is detected by mixed-guinea pig antisera to the same viruses followed by rabbit anti-guinea pig IgG conjugated with peroxidase and subsequently by ortho-phenylenediamine substrate. Titrations of rotavirus (SA11) and adenovirus type 2 tissue culture grown viruses in the presence of separate capture sera and mixed detector sera revealed that the two virus-antibody reactions occurred independently and were not affected by each other. Comparison with another enzyme immunoassay for rotavirus, with immunoelectron microscopy and with polyacrylamide gel electrophoresis showed that EIARA is a sensitive and specific method for detecting rotaviruses and adenoviruses in faeces from children with gastroenteritis.
Pereira
H G
HG
Azeredo
R S
RS
Leite
J P
JP
Andrade
Z P
ZP
De Castro
L
L
D000260:Adenoviruses, Human / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D006168:Guinea Pigs; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D011817:Rabbits; D012401:Rotavirus / Q000276:immunology* / Q000302:isolation & purification
D000956:Antigens, Viral