Intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides.
The intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides was examined by polyacrylamide gel electrophoresis of [35S]methionine-labeled cell extracts under reducing conditions. All of the virion structural proteins were detected in cell extracts, including: L, 180,000 molecular weight (180K); P, 83K; HN, 69K; NP, 66K; F0, 60K; F1, 51K; and M, 38K. P and NP were phosphorylated. HN and F were glycosylated. The kinetics of intracellular viral protein synthesis did not detect any early or late proteins. Pulse-chase experiments failed to detect any precursor-product relationships. No nonstructural proteins were detected.
Wechsler
S L
SL
Lambert
D M
DM
Galinski
M S
MS
Pons
M W
MW
D002460:Cell Line; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D010224:Parainfluenza Virus 3, Human / Q000378:metabolism*; D010766:Phosphorylation; D018108:Respirovirus / Q000378:metabolism*; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000096:biosynthesis*
D006023:Glycoproteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins