Amino acid uptake and protein synthesis in rat testes: stimulation by dissociable factors.
Low concentration (25 mM) of sodium in the incubation medium produced a decrease in the amino acid uptake by the testis tissue as well as a reduction in the response to FSH. In this experimental condition, the basal protein synthesis and the stimulatory effect of FSH was not modified. The subcutaneous administration of testosterone to 15 day old rats increased the protein synthesis in the testis without any modification in the amino acid uptake. The addition of DBcAMP (1 mM) or glucose (14 mM) to the incubation medium increased the protein synthesis in the testes of immature (12 day-old) or prepubertal (32 day-old) rats respectively. The amino acid uptake was not modified. In immature rat testes, with protein synthesis completely inhibited by cycloheximide, the restoration of the sodium concentration in the incubation medium to normal levels produced an increase in amino acid uptake. The results above seem to indicate that protein synthesis and amino acid uptake in rat testes tissue can be regulated, at least partially, by different factors.
Spritzer
P M
PM
Wassermann
G F
GF
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology
D000596:Amino Acids; D013739:Testosterone; D003994:Bucladesine; D005640:Follicle Stimulating Hormone; D003513:Cycloheximide; D012964:Sodium; D005947:Glucose