Suppression of immune cell function in vitro by recombinant human transforming growth factor-beta.
The influence of recombinant human transforming growth factor-beta (rHuTGF-beta) on B-cell function and antigen-specific T-cell responses in vitro was investigated. The addition of 0.1 ng/ml of rHuTGF-beta to cultures of peripheral blood mononuclear cells (PBMC) stimulated with tetanus toxoid resulted in a 50% inhibition of the PBMC proliferative response as determined by [3H]thymidine incorporation. Further, rHuTGF-beta at 0.37 ng/ml caused a greater than 50% reduction in the number of immunoglobulin G-secreting cells among PBMC induced by pokeweed mitogen. rHuTGF-beta also inhibited the generation of allospecific cytotoxic T lymphocytes (CTL) in the mixed-lymphocyte reaction but had no effect on the cytolytic function of CTL generated in the absence of exogenous HuTGF-beta. The results indicate additional immunoregulatory activities for HuTGF-beta and suggest that this factor may play an important role in the regulation of the antigen-dependent immune response.
Shalaby
M R
MR
Department of Pharmacological Sciences, Genentech, Inc., South San Francisco, California 94080.
Ammann
A J
AJ
D000921:Antibody-Producing Cells / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D010455:Peptides / Q000494:pharmacology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013745:Tetanus Toxoid / Q000494:pharmacology; D015291:Transforming Growth Factors
D006133:Growth Substances; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D010455:Peptides; D011043:Pokeweed Mitogens; D011994:Recombinant Proteins; D013745:Tetanus Toxoid; D015291:Transforming Growth Factors