[Natural interferon inducers: double-stranded RNA of killer plasmids of Saccharomyces cerevisiae].
Methods for isolation and purification of yeast double spiral RNA (dsRNA) are described. The most simple method includes reprecipitation of dsRNA in solutions of lithium chloride and its interphase distribution in the system of chloroform: isoamilic alcohol: water. Preparations with the content (w/w) of dsRNA from 30 to 90 per cent were obtained. They possess a high interferon-inducing activity and an antiviral effect on the experimental infection caused by the virus of mouse encephalomyocarditis.
Duzhak
A B
AB
Kostomakha
A N
AN
Lobova
N N
NN
Podgornyĭ
V F
VF
Nosik
N N
NN
D000818:Animals; D004686:Encephalomyocarditis virus; D004769:Enterovirus Infections / Q000628:therapy; D066298:In Vitro Techniques; D007369:Interferon Inducers / Q000302:isolation & purification*; D007370:Interferon Type I / Q000096:biosynthesis / Q000627:therapeutic use; D051379:Mice; D010957:Plasmids; D012330:RNA, Double-Stranded / Q000302:isolation & purification*; D012331:RNA, Fungal / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D007369:Interferon Inducers; D007370:Interferon Type I; D012330:RNA, Double-Stranded; D012331:RNA, Fungal
Prirodnye induktory interferona: dvuspiral'naia RNK killernykh plazmid Saccharomyces cerevisiae.