Aggregation state of the gonadotropin receptor.
The aggregation state of the gonadotropin receptor has been examined by coupling fluorescence energy transfer donor and acceptor fluorophores to hCG and LH. Energy transfer is observed at low (4 degrees C) but not at high (37 degrees C) temperature. Energy transfer could also be detected with receptor solubilized in the presence of hormone at the lower temperature only. Solubilization of receptor in the absence of hormone and subsequent addition of hormone conjugates revealed no energy transfer. These results are consistent with stabilization of receptor complexes at low temperatures, but presumptive hormone induced receptor dissociation under physiological conditions.
Harmatz
D
D
Ji
T H
TH
Middaugh
C R
CR
D055598:Chemical Phenomena; D002621:Chemistry; D006063:Chorionic Gonadotropin / Q000378:metabolism; D004735:Energy Transfer; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006801:Humans; D007986:Luteinizing Hormone / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011966:Receptors, LHRH; D012235:Rhodamines; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D013696:Temperature; D013861:Thiocyanates
D006063:Chorionic Gonadotropin; D005452:Fluoresceins; D011956:Receptors, Cell Surface; D011966:Receptors, LHRH; D012235:Rhodamines; D013861:Thiocyanates; D007986:Luteinizing Hormone; C027755:rhodamine isothiocyanate; D016650:Fluorescein-5-isothiocyanate