The PLC/PRF/5 human hepatoma cell line. II. Chromosomal assignment of hepatitis B virus integration sites.
The chromosomal sites at which hepatitis B virus (HBV) DNA is integrated into the genome of the hepatocellular carcinoma (HCC) cell line, PLC/PRF/5 were investigated in an attempt to understand the mechanisms by which hepatitis B virus may induce malignant transformation. In situ hybridization of an HBV DNA probe to metaphase chromosomes of the PLC/PRF/5 cell line, followed by statistical analysis, identified three integration sites; these were 15q22-q23, 11q22, and 18q12. In particular, hybridization to chromosome #15, which is present in four copies in complete metaphases of this cell line, was highly significant (p much less than 0.0005).
Bowcock
A M
AM
Pinto
M R
MR
Bey
E
E
Kuyl
J M
JM
Dusheiko
G M
GM
Bernstein
R
R
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000382:microbiology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002901:Chromosomes, Human, 13-15; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D005819:Genetic Markers; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics* / Q000382:microbiology; D008297:Male; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral; D005819:Genetic Markers