Nucleotide sequence of a herpes simplex virus type 1 gene that causes cell fusion.
The nucleotide sequence (2041 nucleotides) of a genomic region of herpes simplex virus type 1 (KOS strain) associated with virus-induced cell fusion has been determined. The sequence is bounded by a NruI site at 0.732 and a BamHI site at 0.745 prototypic map units. An open reading frame in the left-to-right orientation specifies a protein of 338 amino acids. The protein is positively charged. Since secondary structure analysis predicts four extensive hydrophobic domains the protein is probably a membrane-associated or a transmembrane protein. Transcription of the putative fusion gene is dependent on viral DNA synthesis, characteristic of the late (gamma) viral gene class. Two syncytia-inducing mutations, syn20 and MP, have been previously mapped to a 504-base pair PstI fragment within these genomic coordinates (V. C. Bond and S. Person (1984), Virology 132, 368-376). The nucleotide sequence of the PstI fragment was determined for the two mutants. Both were shown to have an amino acid substitution at residue 40 of the fusion protein. A second change at residue 101 for MP is probably unrelated to the fusion phenotype.
Debroy
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C
Pederson
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Person
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S
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D002459:Cell Fusion; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D009154:Mutation; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics; D014760:Viral Fusion Proteins
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins