[Effect of lithium on the proliferation of fibroblasts and tumor cell lines in vitro].
The specificity of a proliferation-inducing effect of lithium was investigated. Cell lines of embryonal and adult solid tumors as well as fibroblasts were cultured in lithium-concentrations ranging from 0,5 to 5,0 mmol/l. Neuroblastoma-cell-lines SK-N-SH, SK-N-LO, IMR 32, osteosarcoma-cell-line SAOS 2, melanoma-line IgR 3 and a fibroblast-line were used in this study. Cell proliferation was measured with a 3H-TdR-incorporation-assay and a tumor-stem cell-assay, the fibroblast-proliferation was measured following growth as monolayer respectively. No stimulation of proliferation was observed. The data of this in vitro study are basic for the clinical evaluation of the benefit of lithium in attenuation of chemotherapy induced leukopenia in patients with solid tumors.
Bruchelt
G
G
Buck
J
J
Burdach
S E
SE
Treuner
J
J
D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002712:Chlorides / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008094:Lithium / Q000494:pharmacology*; D018021:Lithium Chloride; D014410:Tumor Stem Cell Assay
D002712:Chlorides; D008094:Lithium; D018021:Lithium Chloride
Der Einfluss von Lithium auf die Proliferation von Fibroblasten- und Tumorzellinien in vitro.