Gene expression in E. coli after treatment with streptozotocin.
Gene induction by the methylating agents streptozotocin (STZ), N-methyl-N-nitrosourea (MNU), and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was evaluated in E. coli fusion mutants. These mutants have fusions of the lac operon to genes induced by treatment with sublethal levels of alkylating agents and were previously selected from random insertions of the Mu-dl (Apr lac) phage by screening for induction of beta-galactosidase activity in the presence of methyl methanesulfonate or MNNG. The results demonstrate that STZ differs from MNNG and MNU in failing to induce aidC expression. Further, expression of aidC after exposure to MNU and MNNG occurs only in nonaerated cultures; aeration blocks the induction. Induction of aidD, alkA, aidB, and sfiA expression occurs with all 3 agents although at markedly lower concentrations of MNNG and STZ compared to MNU. alkA and to a lesser extent aidD mutants of E. coli strains were more sensitive to these agents, while no differences were evident between wild-type and aidB or aidC fusion mutants.
Fram
R J
RJ
Departments of Medicine, University of Massachusetts Medical School, Worcester 01605.
Marinus
M G
MG
Volkert
M R
MR
D000477:Alkylating Agents / Q000494:pharmacology*; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D005798:Genes, Bacterial; D008745:Methylation; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008770:Methylnitrosourea / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013311:Streptozocin / Q000494:pharmacology*; D015533:Transcriptional Activation
D000477:Alkylating Agents; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D008769:Methylnitronitrosoguanidine; D013311:Streptozocin; D008770:Methylnitrosourea