title: Characteristics of benzodiazepine receptor binding in living cultures of mouse cerebral cortex at physiologic temperature.
abstract: Benzodiazepine (BDZ) receptor binding was assayed in situ in dispersed cultures of fetal mouse cerebral cortex at 37 degrees C and at 0 degrees C and compared. Contrary to data obtained from disrupted tissues, receptor binding at physiologic temperature was reduced only to 40% of that observed in the cold when intact tissues were used for assay. Neuronal (clonazepam-displaceable) receptor binding was essentially unaffected by changes in temperature; however, non-neuronal (Ro5-4864-displaceable) binding was reduced to 25% of that of 0 degrees C. A relatively high-affinity (Kd approximately 24 nM) as well as a low-affinity (Kd approximately 200 nM) binding site were identified; the numbers of binding sites were only modestly reduced at physiologic temperature and probably reflect a predominant reduction in non-neuronal sites.
authors.forename: P K
authors.initials: PK
authors.lastname: Sher
mesh_terms: D000818:Animals; D001570:Benzodiazepinones / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D002998:Clonazepam / Q000378:metabolism; D046508:Culture Techniques; D007700:Kinetics; D051379:Mice; D011963:Receptors, GABA-A / Q000378:metabolism*; D013696:Temperature
chemical_list: D001570:Benzodiazepinones; D011963:Receptors, GABA-A; C028513:4'-chlorodiazepam; D002998:Clonazepam