Translational signals of a major head protein gene of bacteriophage lambda.
The D gene of bacteriophage lambda which codes for a major head protein is expressed at a high level during lytic growth. We have constructed a set of D-lacZ gene fusions in order to examine the factors determining the high efficiency of the D translational initiation signals. It was found that an integral sequence, 300 bp long and upstream of the ATG initiation codon, is required for maximal protein synthesis.
Suissa
M
M
Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Altuvia
S
S
Koby
S
S
Giladi
H
H
Oppenheim
A B
AB
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D005822:Genetic Vectors; D010957:Plasmids; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D014764:Viral Proteins; D001616:beta-Galactosidase