[Characterization and differentiation of rat preadipocytes in primary culture].
Using a density gradient medium, we isolated homogeneous cell populations from the inguinal tissue of 3-day old rats. In primary culture we obtained, for the first time, the differentiation of 99% of the cells in the presence of a physiological concentration of insulin (10(-9) M). This model closely mimicked the events occurring during normal mammalian adipose development, i.e. a positive change in beta-adrenergic sensitivity, early induction of lipoprotein lipase, expression of the enzymes involved in the triglyceride systems, and the development of responsiveness to glucagon.
Gaben-Cogneville
A M
AM
Quignard-Boulangé
A
A
Aron
Y
Y
Brigant
L
L
Jahchan
T
T
Pello
J Y
JY
Poussin
B
B
Swierczewski
E
E
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D005934:Glucagon / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000494:pharmacology; D051381:Rats; D013234:Stem Cells / Q000166:cytology; D014280:Triglycerides / Q000378:metabolism
D007328:Insulin; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D005934:Glucagon; D000242:Cyclic AMP; D008071:Lipoprotein Lipase; D007545:Isoproterenol
Caractérisation et différenciation de préadipocytes de rats en culture primaire.