A herpes simplex virus type 1 mutant with a deletion in the polypeptide-coding sequences of the ICP4 gene.
A deletion mutant derived from herpes simplex virus type 1 (HSV-1) strain ANG was analysed. The deletion mapped within the polypeptide-coding region of the immediate-early ICP4 gene. Based on DNA sequence data the deletion was shown to comprise 84 base pairs. In the wild-type genome of strain ANG these sequences were almost completely homologous to the known sequences of HSV-1 strain 17. The ICP4 polypeptide induced by the mutant was similar in size to the wild-type ICP4 protein and was recognized by a monoclonal antibody against ICP4. The data presented suggest that the deletion corresponds to a region on the ICP4 polypeptide that is nonessential for the replication of the virus in vitro.
Schröder
C H
CH
DeZazzo
J
J
Knopf
K W
KW
Kaerner
H C
HC
Levine
M
M
Glorioso
J
J
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D005796:Genes; D005814:Genes, Viral; D009154:Mutation; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral; D010455:Peptides; D014764:Viral Proteins; D004262:DNA Restriction Enzymes