IL-1 is an autocrine growth factor for T cell clones.
Activation of Th lymphocytes requires that Ag be presented on the surface of accessory cells displaying Ia Ag. A number of studies have concluded that the T cell also requires IL-1 from accessory cells. However, we recently reported that one murine T cell clone (D10.G4.1) produced its own IL-1-like activity after encountering APC (9). In this report, we demonstrate that 1) IL-1 production is a common property of murine T cell clones, 2) T cell IL-1 activity is blocked by anti-IL-1-alpha antiserum, 3) IL-1-alpha mRNA can be directly visualized in individual cloned T cells using in situ hybridization techniques, and 4) IL-1 appears to serve an autocrine role in the activation of T cell clones inasmuch as anti-IL-1-alpha antiserum blocks cell proliferation when the T cell is the only IL-1 source.
Tartakovsky
B
B
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701.
Finnegan
A
A
Muegge
K
K
Brody
D T
DT
Kovacs
E J
EJ
Smith
M R
MR
Berzofsky
J A
JA
Young
H A
HA
Durum
S K
SK
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive; D002999:Clone Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D007106:Immune Sera; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology*; D007424:Intracellular Fluid / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology* / Q000276:immunology / Q000378:metabolism
D007106:Immune Sera; D007375:Interleukin-1; D012333:RNA, Messenger