Identification by immunofluorescence of ACTH-producing cells in the pituitary gland of the tree frog Hyla arborea.
The indirect immunofluorescence procedure was used to localize ACTH-producing cells in the pituitary distal lobe from Hyla arborea adult specimens; the antiserum employed was rabbit anti-synthetic ACTH (1-24) conjugated with BSA. Immunohistochemical staining was suppressed by solid-phase absorption of the anti-ACTH with the specific antigen. In the distal lobe one cell population is immunoreactive; these cells are predominantly localized in the anterior half, both ventrally and dorsally; they exclusively correspond to type-3 basophils. In the intermediate lobe the total cell population is immunoreactive. Parallel inspections carried out on the pituitary of Xenopus laevis specimens gave results fully consistent with those reported by other authors.
Campantico
E
E
Guastalla
A
A
Patriarca
E
E
D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis*; D000818:Animals; D001001:Anura; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000166:cytology
D007106:Immune Sera; D000324:Adrenocorticotropic Hormone