Independent lateral diffusion of cytochrome bc1 complex and cytochrome oxidase in the mitochondrial inner membrane.
Distinct fluorophores have been conjugated to antibodies for cytochrome bc1 complex and cytochrome oxidase, two integral electron transferring proteins in the mitochondrial inner membrane. Addition of these fluorescent antibodies to preparations of mitochondrial inner membranes followed by appropriate secondary antibodies causes distinct and independent aggregation of the two cytochrome proteins. These results reveal that both cytochrome bc1 complex and cytochrome oxidase diffuse laterally in the membrane plane independent of one another consistent with the random collision model for electron transport in the mitochondrial inner membrane.
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D000818:Animals; D004058:Diffusion; D004579:Electron Transport; D014450:Electron Transport Complex III; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007425:Intracellular Membranes / Q000502:physiology; D008560:Membrane Fluidity; D008930:Mitochondria, Liver / Q000502:physiology* / Q000648:ultrastructure; D009097:Multienzyme Complexes / Q000502:physiology*; D009247:NADH, NADPH Oxidoreductases / Q000502:physiology*; D011808:Quinone Reductases / Q000502:physiology*; D051381:Rats
D009097:Multienzyme Complexes; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases; D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III