title: Differential phosphorylation of multiple sites in protein 4.1 and protein 4.9 by phorbol ester-activated and cyclic AMP-dependent protein kinases.
abstract: The phosphorylation of the membrane skeleton components protein 4.1 and protein 4.9 in intact erythrocytes is shown to increase in the presence of either 1 microM 12-O-tetradecanoyl phorbol 13-acetate or 2 mM dibutyryl cAMP. The phosphorylation induced by these protein kinase activators is compared by two-dimensional tryptic peptide mapping. In both proteins, the pattern of peptides phosphorylated in the presence of 12-O-tetradecanoyl phorbol 13-acetate differs from the pattern of peptides phosphorylated in the presence of dibutyryl cAMP. The relative locations of the phosphorylated sites on protein 4.1 have been determined using limited proteolysis by alpha-chymotrypsin.
authors.forename: W C
authors.initials: WC
authors.lastname: Horne
authors.forename: T L
authors.initials: TL
authors.lastname: Leto
authors.forename: V T
authors.initials: VT
authors.lastname: Marchesi
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology*; D003598:Cytoskeletal Proteins; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009479:Neuropeptides; D010446:Peptide Fragments / Q000032:analysis; D010704:Phorbols / Q000494:pharmacology*; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011494:Protein Kinases / Q000097:blood*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D001798:Blood Proteins; D003598:Cytoskeletal Proteins; C044805:DMTN protein, human; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009479:Neuropeptides; D010446:Peptide Fragments; D010704:Phorbols; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1; D003994:Bucladesine; D011494:Protein Kinases; D013755:Tetradecanoylphorbol Acetate