Activation of actin-containing microfilaments by vasopressin in the amphibian urinary bladder epithelium: a fluorescent study using NBD-phallacidin.
The effect of an antidiuretic hormone (ADH, vasopressin) on the microfilament system of the toad urinary bladder lumenal epithelium was investigated using NBD-phallacidin (NBD-ph). The latter material is a specific fluorescent label for F actin. In the presence of an osmotic gradient, both ADH and cyclic adenosine monophosphate (cAMP) appear to induce the polymerization of monomeric actin into F actin-containing microfilaments. The latter may then be involved in the morphological changes, including the formation of lateral intercellular lakes, associated with the typical hydroosmotic response.
Davis
W L
WL
Jones
R G
RG
Richemont
P C
PC
Goodman
D B
DB
D000199:Actins / Q000378:metabolism*; D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002024:Bufo marinus / Q000502:physiology*; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects* / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects; D005260:Female; D008856:Microscopy, Fluorescence; D010456:Peptides, Cyclic; D013873:Thionucleotides / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects*
D000199:Actins; D000546:Amanitins; D010456:Peptides, Cyclic; D013873:Thionucleotides; D001127:Arginine Vasopressin; C017613:phallacidin; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; C028877:7-nitrobenz-2-oxa-1,3-diazole-phallacidin; D000242:Cyclic AMP