title: Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon.
abstract: The preceding paper presents evidence for the co-transcriptional expression of the ileS and lsp genes in Escherichia coli. To identify the promoter for the ileS-lsp operon, we have determined the nucleotide sequence of an 1.8-kilobase DNA fragment between the rpsT and IleS genes. The sequence data have revealed an open reading frame, designated gene X, which encodes a polypeptide with 312 amino acid residues. Both in vivo and in vitro expressions of the x gene result in the synthesis of a soluble protein with an apparent Mr of 35,000. The x gene is transcribed in the same direction as that of the ileS-lsp operon and opposite to that of the upstream adjacent rpsT gene. No transcription termination sequence can be discerned in the intercistronic region between the x and ileS genes. DNase I footprinting experiment revealed a RNA polymerase binding site at 170-151 base pairs upstream of the x gene.
authors.forename: Y
authors.initials: Y
authors.lastname: Kamio
authors.forename: C K
authors.initials: CK
authors.lastname: Lin
authors.forename: M
authors.initials: M
authors.lastname: Regue
authors.forename: H C
authors.initials: HC
authors.lastname: Wu
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D009876:Operon; D010957:Plasmids
chemical_list: D001426:Bacterial Proteins; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII; C024406:endodeoxyribonuclease HpaI; D015252:Deoxyribonucleases, Type II Site-Specific