[Stimulation of the chemiluminescence of human polymorphonuclear leukocytes in various stages of the intraerythrocyte development of Plasmodium falciparum].
The synchronized cultures of Plasmodium falciparum were used to stimulate in vitro the chemiluminescence of human polymorphonuclear leukocytes in the presence of immune serum. The schizonts were concentrated by Percoll gradient centrifugation method (density 1.085 and osmolarity 285 mOsmol), and placed in culture, treated 6 hours later by sorbitol. Under incubation at constant temperature and pressure, the rate of synchronization reached 85% for schizonts during 5 replicative cycles. Every asexual stages of Plasmodium falciparum were used separately to stimulate polymorphonuclear leukocytes: merozoites were the most effective, followed by schizonts, trophozoites, and lastly supernatants of cultures containing degradation products of parasites.
Malhotra
K
K
Hôpital Claude Bernard, Paris.
Salmon
D
D
Le Bras
J
J
Savel
J
J
Vilde
J L
JL
D000818:Animals; D006801:Humans; D008163:Luminescent Measurements; D008288:Malaria / Q000097:blood*; D009504:Neutrophils / Q000378:metabolism* / Q000469:parasitology; D010963:Plasmodium falciparum / Q000254:growth & development*; D013012:Sorbitol / Q000494:pharmacology; D013997:Time Factors
D013012:Sorbitol
Stimulation de la chemiluminescence des polynucléaires humains par différents stades du développement intraérythrocytaire de Plasmodium falciparum.