The structural gene for a phosphorus-repressible phosphate permease in Neurospora crassa can complement a mutation in positive regulatory gene nuc-1.
van+, a gene encoding a phosphorus-repressible phosphate permease, was isolated by its ability to complement nuc-1, a positive regulatory locus that normally regulates van+ expression. This was unexpected because the nuc-1 host already contained a resident van+ gene. Plasmids carrying van+ complemented a nuc-2 mutation as well. Probing of RNA from untransformed wild-type (nuc-1+) and constitutive (nuc-1c) strains by van+ probes indicated that levels of the van+ transcript were subject to control by nuc-1+. Probing of the same RNAs with a cosmid clone, containing approximately 15 kilobases of upstream and downstream DNA, revealed no other detectable phosphorus-regulated transcripts within this 40-kilobase region of the chromosome.
Mann
B J
BJ
Department of Physiological Chemistry, University of Wisconsin, Madison 53706.
Akins
R A
RA
Lambowitz
A M
AM
Metzenberg
R L
RL
D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005796:Genes; D005800:Genes, Fungal; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D026901:Membrane Transport Proteins / Q000235:genetics*; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D028061:Phosphate Transport Proteins; D010710:Phosphates / Q000378:metabolism*; D010758:Phosphorus / Q000494:pharmacology; D010957:Plasmids; D012331:RNA, Fungal / Q000235:genetics; D014158:Transcription, Genetic; D014638:Vanadates / Q000378:metabolism
D026901:Membrane Transport Proteins; D028061:Phosphate Transport Proteins; D010710:Phosphates; D012331:RNA, Fungal; D010758:Phosphorus; D014638:Vanadates; C055831:phosphate permease