title: Role of phospholipids in the binding activity of vasoactive intestinal peptide receptors.
abstract: Phospholipase digestion of rat intestinal epithelial cell membranes was performed in order to study the influence of membrane phospholipids on the binding activity of VIP receptors. Phospholipases A2 and C strongly (ED50 congruent to 4 X 10(-2) and 4 X 10(-1) micrograms/ml, respectively) and rapidly reduced 125I-VIP binding to membranes whereas phospholipase D was ineffective. This suggests an important role of both hydrophobic and hydrophilic groups of phospholipids on VIP receptor binding activity.
authors.forename: A
authors.initials: A
authors.lastname: Sarrieau
authors.forename: N
authors.initials: N
authors.lastname: Boige
authors.forename: M
authors.initials: M
authors.lastname: Laburthe
mesh_terms: D000818:Animals; D007413:Intestinal Mucosa / Q000378:metabolism*; D008563:Membrane Lipids / Q000502:physiology; D010740:Phospholipases; D010743:Phospholipids / Q000502:physiology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018005:Receptors, Vasoactive Intestinal Peptide; D013329:Structure-Activity Relationship; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D008563:Membrane Lipids; D010743:Phospholipids; D011956:Receptors, Cell Surface; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D010740:Phospholipases