Plasminogen activation: biochemistry, physiology, and therapeutics.
The mammalian serine protease zymogen, plasminogen, can be converted into the active enzyme plasmin by vertebrate plasminogen activators urokinase (uPA), tissue plasminogen activator (tPA), factor XII-dependent components, or by bacterial streptokinase. The biochemical properties of the major components of the system, plasminogen/plasmin, plasminogen activators, and inhibitors of the plasminogen activators, are reviewed. The plasmin system has been implicated in a variety of physiological and pathological processes such as fibrinolysis, tissue remodeling, cell migration, inflammation, and tumor invasion and metastasis. A defective plasminogen activator/inhibitor system also has been linked to some thromboembolic complications. Recent studies of the mechanism of fibrinolysis in human plasma suggest that tPA may be the primary initiator and that overall fibrinolytic activity is strongly regulated at the tPA level. A simple model for the initiation and regulation of plasma fibrinolysis based on these studies has been formulated. The plasminogen activators have been used for thrombolytic therapy. Three new thrombolytic agents--tPA, pro-uPA, and acylated streptokinase-plasminogen complex--have been found to possess better properties over their predecessors, urokinase and streptokinase. Further improvements of these molecules using genetic and protein engineering tactics are being pursued.
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Department of Biological Sciences, Monsanto Co., Chesterfield, Missouri.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005341:Fibrinolysin / Q000502:physiology; D005342:Fibrinolysis; D006023:Glycoproteins / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D010958:Plasminogen / Q000502:physiology*; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors / Q000502:physiology / Q000627:therapeutic use; D015849:Plasminogen Inactivators
D006023:Glycoproteins; D015849:Plasminogen Inactivators; D010958:Plasminogen; D010960:Plasminogen Activators; D005341:Fibrinolysin