title: Liquid chromatographic analysis of doxazosin in human serum with manual and robotic sample preparation.
abstract: A specific method for the determination of the antihypertensive drug doxazosin in human serum is described. The method utilizes the related drug prazosin as an internal standard and is based on a simple extraction scheme followed by analysis by reversed-phase ion suppression high-performance liquid chromatography (HPLC) on an alumina-based column with fluorescence detection. The method is completely automated with a flexible robotic system for the analysis of drugs in biological fluids. The robotic automation of the method allows a 20% increase in the sample throughput and the savings of about 7 man-hours a day. Both the manual and robotic procedures yield precise quantitative results over the therapeutically relevant concentration range of 0.5 to 20 ng/mL of serum.
authors.affiliation: Central Research, Pfizer Inc., Groton, Connecticut 06340.
authors.forename: H G
authors.initials: HG
authors.lastname: Fouda
authors.forename: T M
authors.initials: TM
authors.lastname: Twomey
authors.forename: R P
authors.initials: RP
authors.lastname: Schneider
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D017292:Doxazosin; D006801:Humans; D011224:Prazosin / Q000031:analogs & derivatives* / Q000097:blood; D012371:Robotics; D013050:Spectrometry, Fluorescence
chemical_list: D017292:Doxazosin; D011224:Prazosin