title: cAMP induces a transient elevation of cGMP levels during early culmination of Dictyostelium minutum.
abstract: Oscillatory cAMP signals very likely organize the cell movement which leads to fruiting body construction in Dictyostelium minutum [Schaap, P., Konijn, T.M. and Van Haastert, P.J.M.: Proc. Natl. Acad. Sci. USA 81, 2122-2126 (1984)]. Stimulation with cAMP induces a transient elevation of cGMP in cells at the early culmination stage, which peaks at 12-18 s. A half maximal cGMP response is induced by 10(-7) M cAMP and saturation of the response is reached at 10(-5) M cAMP. No cGMP accumulation was induced by stimulation of vegetative or aggregative cells of D. minutum by cAMP. Since the transient increase of cGMP is most likely involved in the transduction of chemotactic signals, our results indicate that cAMP signals organize fruiting body formation by inducing chemotaxis inside the aggregate.
authors.forename: P
authors.initials: P
authors.lastname: Schaap
authors.forename: M
authors.initials: M
authors.lastname: Wang
mesh_terms: D002633:Chemotaxis / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000378:metabolism*; D004023:Dictyostelium / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000242:Cyclic AMP; D006152:Cyclic GMP