Expression of transfected DNA in avian cells can be enhanced in trans by retroviral infection.
Using a quantitative S1 nuclease protection assay, we demonstrated that acute or chronic infection of avian cells enhances expression of an exogenously introduced rat preproinsulin II gene by approximately equal to 50-fold. The degree of enhancement is shown to vary with the transfection technique used but is independent of the transcription control region of the transfected gene. We conclude that retroviral infection of avian cells enhances expression of transfected DNA in trans by facilitating the uptake of DNA rather than by activating the transfected promoter.
Cullen
B R
BR
Katz
R A
RA
Ju
G
G
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D005786:Gene Expression Regulation; D007328:Insulin; D011384:Proinsulin / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics; D011784:Quail; D012192:Retroviridae Infections / Q000235:genetics*; D014162:Transfection
D007328:Insulin; D011498:Protein Precursors; C013737:preproinsulin; D004247:DNA; D011384:Proinsulin