[Rearrangement of the proto-oncogene c-myc in fresh cells from Burkitt's leukemia (L3)].
The presence of a rearrangement of the proto-oncogene c-myc was investigated in the DNA of fresh cells isolated from the blood of two patients with Burkitt's leukemia (L3), and from the node biopsy of a patient with Burkitt's lymphoma. Both samples from the L3 leukemia patients had the characteristic t(8;14) translocation, while the lymphoma specimen presented no abnormality of chromosome 8. Only one of the leukemic DNA's presented a rearranged c-myc pattern, with the breakpoint region located between the first and the second exon. The c-myc pattern of the two other patients appeared normal. The finding of a rearranged c-myc oncogene in fresh cells from a Burkitt's leukemia is direct evidence for the implication of c-myc in the disease whereas most of the rearrangements previously described have been found in cell lines established in culture.
Mathieu-Mahul
D
D
Bernheim
A
A
Berger
R
R
Mauchauffe
M
M
Flandrin
G
G
Larsen
C J
CJ
D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics; D002906:Chromosomes, Human, 6-12 and X; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D014178:Translocation, Genetic
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004247:DNA; D004262:DNA Restriction Enzymes
Remaniement du proto-oncogène c-myc dans les cellules fraîches d'une leucémie de Burkitt (L3).