Beta-endorphin-like immunoreactivity in normal mucosa, muscle layer, adenocarcinoma, and polyp of the colon.
beta-Endorphin-like immunoreactivity was detected in the mucosa and muscle layer of normal colon, adenocarcinomas derived from the colon mucosa, and colon polyps which were histologically confirmed to be adenoma without a focus of carcinoma or with in situ carcinoma. The contents of beta-endorphin-like immunoreactivity in adenocarcinomatous tissue (11.94 +/- 1.77 pmol/g wet wt) and colon polyps without focus of carcinoma (10.71 +/- 1.50 pmol/g wet wt) were found to be significantly higher than those in the mucosal layer (6.86 +/- 0.64 pmol/g wet wt) and muscle layer (8.30 +/- 0.68 pmol/g wet wt) of normal colon. These data suggest that the production of beta-endorphin-like immunoreactivity is specifically increased in some adenocarcinomas and adenomatous polyps and may be related to the alteration of bowel habits. Gel exclusion chromatography of beta-endorphin-like immunoreactivity revealed three peaks corresponding to beta-endorphin, beta-lipotropin, and an immunoreactive form between the two. In the mucosal layer and muscle layer of the colon, a broad major peak was eluted at the position of beta-endorphin, and minor peaks were eluted at the position of beta-lipotropin and between beta-endorphin and beta-lipotropin. In adenocarcinoma and polyp, the peak size corresponding to authentic beta-lipotropin was greater than that of beta-endorphin. This study demonstrated that beta-endorphin-like immunoreactivity existed at a high concentration in some colon adenocarcinomas and polyps whose elution patterns were different from those of normal colon tissue.
Tari
A
A
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Miyachi
Y
Y
Sumii
K
K
Haruma
K
K
Yoshihara
M
M
Kajiyama
G
G
Miyoshi
A
A
D000230:Adenocarcinoma / Q000032:analysis; D000328:Adult; D000368:Aged; D002850:Chromatography, Gel; D003106:Colon / Q000032:analysis*; D003110:Colonic Neoplasms / Q000032:analysis*; D003111:Colonic Polyps / Q000032:analysis; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000032:analysis; D011863:Radioimmunoassay; D012016:Reference Values; D001615:beta-Endorphin / Q000032:analysis*; D008083:beta-Lipotropin / Q000032:analysis
D001615:beta-Endorphin; D008083:beta-Lipotropin