Variability of the region of the herpes simplex virus type 1 genome yielding defective DNA: SmaI fragment polymorphism.
A SmaI map of class I defective DNA of herpes simplex virus type 1 (HSV1) was constructed using a set of deletion hybrid phages. Four SmaI fragments on the defective DNA had a variability in length common among 15 HSV1 isolates: the 1.45 kilobase (kb) fragment located within the BamHI-Z (map coordinates 0.936-0.949) fragment, the 0.92-kb fragment neighboring on the 'a' sequence, the 0.44-kb fragment containing the intervening sequence of immediate-early mRNA-5 gene, and the 0.205-kb fragment corresponding to the 'a' sequence. The four SmaI fragments have several sets of reiterated sequences, among which the 1.45- and 0.92-kb fragments hybridized with mammalian cellular DNAs (human, monkey, rabbit, and mouse).
Umene
K
K
D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D003673:Defective Viruses / Q000235:genetics*; D011110:Polymorphism, Genetic; D018139:Simplexvirus / Q000235:genetics*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes