title: Drosophila P element-enhanced transfection in mammalian cells.
abstract: We constructed a gene transfer vector containing the herpes simplex virus type 1 thymidine kinase (TK) gene flanked by Drosophila P element terminal repeats (W. R. Engels, Annu. Rev. Genet. 17:315-344). This vector was introduced into mouse LTK- cells and enhanced the frequency of stable transformation to the TK+ phenotype by approximately 50-fold relative to a similar plasmid lacking the P element terminal repeats.
authors.forename: D W
authors.initials: DW
authors.lastname: Clough
authors.forename: H M
authors.initials: HM
authors.lastname: Lepinske
authors.forename: R L
authors.initials: RL
authors.lastname: Davidson
authors.forename: R V
authors.initials: RV
authors.lastname: Storti
mesh_terms: D000818:Animals; D002460:Cell Line; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005822:Genetic Vectors; D018139:Simplexvirus / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection
chemical_list: D004251:DNA Transposable Elements; D013937:Thymidine Kinase