Mapping parathyroid hormone, beta-globin, insulin, and LDH-A genes within the human chromosome 11 short arm by spot blotting sorted chromosomes.
Rearranged human chromosomes carrying segments of chromosome 11 were separated from the normal chromosome 11 by high-resolution chromosome sorting. Sorted chromosomes were tested with parathyroid hormone, beta-globin, insulin, and LDH-A gene-specific probes to determine the genes carried by each chromosome segment. Based on the gene content and karyotypes of these abnormal chromosomes, the parathyroid hormone, beta-globin, insulin, and LDH-A genes and the unique restriction fragment ADJ-762 are all located on the terminal band of the short arm of human chromosome 11 (band 11p15), with LDH-A proximal to the other loci.
Lebo
R V
RV
Cheung
M C
MC
Bruce
B D
BD
Riccardi
V M
VM
Kao
F T
FT
Kan
Y W
YW
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D004262:DNA Restriction Enzymes; D005819:Genetic Markers; D005914:Globins / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D007328:Insulin / Q000235:genetics*; D007527:Isoenzymes; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D010281:Parathyroid Hormone / Q000235:genetics*
D005819:Genetic Markers; D007328:Insulin; D007527:Isoenzymes; D010281:Parathyroid Hormone; D005914:Globins; D007770:L-Lactate Dehydrogenase; D004262:DNA Restriction Enzymes