Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene.
The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene from Escherichia coli has allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.
Lobel
L I
LI
Patel
M
M
King
W
W
Nguyen-Huu
M C
MC
Goff
S P
SP
D001483:Base Sequence; D004251:DNA Transposable Elements; D004274:DNA, Recombinant / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005814:Genes, Viral; D008979:Moloney murine leukemia virus / Q000235:genetics; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012343:RNA, Transfer / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D013489:Suppression, Genetic
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D004279:DNA, Viral; D012343:RNA, Transfer