title: Thymocyte cyclic AMP and cyclic GMP response to treatment with metabolites issued from the lipoxygenase pathway.
abstract: Evidence has been presented that cGMP is the second messenger for the lipoxygenase metabolites 15-HETE and LTB4 in the mouse splenocyte and thymocyte. Incubation of splenocytes with 10(-7) to 10(-9) M 15-HETE caused a slight decrease in cAMP levels and an increase in cGMP levels after 10 to 20 min. Mature PNA-, immature PNA+, and whole thymocytes treated with 10(-7) to 10(-10) M 15-HETE and 10(-11) M LTB4 showed an approximately 100% increase in cGMP production. In mixed lymphocyte reactions, 15-HETE- and LTB4-treated PNA+, PNA-, and whole thymocyte populations inhibited thymidine uptake by fresh allostimulated splenocytes. These results demonstrate that the eicosanoid-induced generation of suppressor cells follows a rise in lymphocyte cGMP levels.
authors.forename: S
authors.initials: S
authors.lastname: Mexmain
authors.forename: J
authors.initials: J
authors.lastname: Cook
authors.forename: J C
authors.initials: JC
authors.lastname: Aldigier
authors.forename: N
authors.initials: N
authors.lastname: Gualde
authors.forename: M
authors.initials: M
authors.lastname: Rigaud
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis*; D006152:Cyclic GMP / Q000096:biosynthesis*; D005260:Female; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology; D037102:Lectins; D007975:Leukotriene B4 / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D019887:Peanut Agglutinin; D010641:Phenotype; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000145:classification / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D037102:Lectins; D019887:Peanut Agglutinin; D007975:Leukotriene B4; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D000242:Cyclic AMP; D008084:Lipoxygenase; D006152:Cyclic GMP