title: 'Red pigment' from ADE-2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases.
abstract: Protection of DNA from cleavage by restriction endonucleases EcoRI, HindIII, BamHI, and Bg/II with red pigment, produced by ADE-2 mutants of Saccharomyces cerevisiae is demonstrated. Purification of yeast DNA from pigment can be achieved by chromatography on hydroxyapatite columns.
authors.forename: A
authors.initials: A
authors.lastname: Meskauskas
authors.forename: V
authors.initials: V
authors.lastname: Ksenzenko
authors.forename: M
authors.initials: M
authors.lastname: Shlyapnikov
authors.forename: V
authors.initials: V
authors.lastname: Kryukov
authors.forename: D
authors.initials: D
authors.lastname: Citavicius
mesh_terms: D000225:Adenine / Q000096:biosynthesis*; D001426:Bacterial Proteins; D010582:Bacteriophage lambda; D004396:Coloring Agents / Q000494:pharmacology*; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors*; D004271:DNA, Fungal / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004396:Coloring Agents; D004271:DNA, Fungal; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific; D000225:Adenine