The cytotoxic response to murine cytomegalovirus. II. In vitro requirements for generation of cytotoxic T cells.
A cytotoxic response to murine cytomegalovirus (MCMV) was obtained by the culture of lymph node cells from mice inoculated with MCMV into both hind footpads 7 days previously. The cytotoxicity was mediated by Thy1.2+, Lyt2+, H-2-restricted effector cells and was virus-specific. Investigation of the in vitro conditions established that T cell proliferation was necessary for optimal generation of cytotoxicity, that proliferation was dependent upon Thy1.2+, Lyt2+ cell populations and that supernatants from concanavalin A-activated spleen cells enhanced the levels of cytotoxicity obtained.
Sinickas
V G
VG
Ashman
R B
RB
Blanden
R V
RV
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D003165:Complement System Proteins / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D003586:Cytomegalovirus Infections / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000276:immunology / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013696:Temperature
D000961:Antilymphocyte Serum; D003208:Concanavalin A; D003165:Complement System Proteins