title: Acquisition process of differentiation competence in Dictyostelium discoideum.
abstract: It was previously shown [K. Okamoto, J. Gen. Microbiol. 127, 301 (1981)] that Dictyostelium discoideum cells dissociated from early aggregates, but not aggregation competent cells obtained in a suspension culture, undergo prespore differentiation, when transferred into a medium containing glucose, albumin, and cAMP. Therefore, the former, but not the latter, is considered to have been acquired "differentiation competence." In the present work, the requirements for cells to acquire the differentiation competence are investigated with D. discoideum NC4 strain. On solid substratum, the incubation above a threshold density is absolutely required for this process, while cell aggregation itself is not essential. In suspension cultures, the competence is acquired only under hypertonic conditions. Inhibition of protein synthesis or depletion of cAMP does not affect the acquisition process of the competence. The requirement of hypertonic treatment was also investigated with several other D. discoideum strains.
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000643:Ammonium Chloride / Q000494:pharmacology; D002449:Cell Aggregation; D002454:Cell Differentiation; D003470:Culture Media; D000242:Cyclic AMP / Q000502:physiology; D004023:Dictyostelium / Q000166:cytology*; D005656:Fungal Proteins / Q000096:biosynthesis; D009994:Osmolar Concentration; D006005:Phosphorylases / Q000378:metabolism; D013172:Spores, Fungal
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D000643:Ammonium Chloride; D000242:Cyclic AMP; D006005:Phosphorylases; D015105:3',5'-Cyclic-AMP Phosphodiesterases