title: The accessibility of phage MS2 RNA to structure specific nucleases in various conditions.
abstract: The accessibility of ds- and ss-segments of phage MS2 RNA to ds- and ss-specific nucleases (RNase III, nuclease SV and nuclease S1) was studied. The results show that the RNA has hydrolysis sites for all the nucleases used. These sites are unvariable in a wide range of the conditions (ionic strength, pH, bivalent cations and temperature) and are not changed also after denaturation-renaturation of the RNA. This testifies that the distribution and interactions of ds- and ss-segments in the whole molecule are very specific and stable.
authors.forename: V V
authors.initials: VV
authors.lastname: Grechko
authors.forename: G I
authors.initials: GI
authors.lastname: Tymokhina
authors.forename: L A
authors.initials: LA
authors.lastname: Aleshkina
authors.forename: J I
authors.initials: JI
authors.lastname: Kalashnikova
authors.forename: S P
authors.initials: SP
authors.lastname: Majev
authors.forename: S K
authors.initials: SK
authors.lastname: Vassilenko
mesh_terms: D001435:Bacteriophages / Q000378:metabolism*; D001665:Binding Sites; D004720:Endonucleases / Q000378:metabolism; D004722:Endoribonucleases / Q000378:metabolism; D006868:Hydrolysis; D012330:RNA, Double-Stranded / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D043244:Ribonuclease III; D012260:Ribonucleases / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D004720:Endonucleases; D004722:Endoribonucleases; D012260:Ribonucleases; C043004:cobra venom endonuclease; D043244:Ribonuclease III; D015719:Single-Strand Specific DNA and RNA Endonucleases