title: Atrial natriuretic polypeptide in bovine adrenal medulla.
abstract: Two radioimmunoassays for alpha-human atrial natriuretic polypeptide (alpha-hANP) with different specificities were used to study the tissue level and the nature of alpha-hANP-like immunoreactivity in the bovine adrenal gland. A considerable amount of alpha-hANP-like immunoreactivity was detected in the adrenal medulla (90.8 +/- 21.1 and 90.0 +/- 23.1 ng/g with the two radioimmunoassays), while no detectable amount (less than 1.0 ng/g) was present in the cortex. Gel permeation chromatographic analysis showed that ANP in the medulla is composed of two components of alpha-hANP-like immunoreactivity with high and low molecular weights in the approximate ratio of 2:1, eluting at the elution positions of gamma-hANP and alpha-hANP, respectively. Reverse-phase high performance liquid chromatographic analysis revealed that alpha-hANP-like immunoreactivity with a low molecular weight in the medulla consists of two major components, which comigrate with synthetic alpha-hANP(5-28) and alpha-hANP. When cultured bovine adrenal chromaffin cells were incubated in the presence of nicotine (10(-5) M), alpha-hANP-like immunoreactivity was released into the medium concomitantly with catecholamines from chromaffin cells. These findings indicate that a discrete ANP system is present in the adrenal medulla and that ANP is cosecreted with catecholamines from chromaffin cells, suggesting the possible involvement of ANP in the adrenomedullary function.
authors.affiliation: Department of Medicine, Kyoto University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Mukoyama
authors.forename: K
authors.initials: K
authors.lastname: Nakao
authors.forename: N
authors.initials: N
authors.lastname: Morii
authors.forename: S
authors.initials: S
authors.lastname: Shiono
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: A
authors.initials: A
authors.lastname: Sugawara
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Arai
authors.forename: H
authors.initials: H
authors.lastname: Imura
mesh_terms: D000302:Adrenal Cortex / Q000032:analysis; D000313:Adrenal Medulla / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D002395:Catecholamines / Q000032:analysis / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D002837:Chromaffin Granules / Q000187:drug effects / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D009538:Nicotine / Q000494:pharmacology; D011863:Radioimmunoassay
chemical_list: D002395:Catecholamines; D009538:Nicotine; D009320:Atrial Natriuretic Factor