Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs.
Two distinct but distantly related complementary DNAs encoding proteins sharing human interleukin-1 (IL-1) activity (termed IL-1 alpha and IL-1 beta), were isolated from a macrophage cDNA library. The primary translation products of the genes are 271 and 269 amino acids long, although expression in Escherichia coli of the carboxy-terminal 159 and 153 amino acids produces IL-1 biological activity.
March
C J
CJ
Mosley
B
B
Larsen
A
A
Cerretti
D P
DP
Braedt
G
G
Price
V
V
Gillis
S
S
Henney
C S
CS
Kronheim
S R
SR
Grabstein
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000302:isolation & purification; D014981:Xenopus
D004270:DNA, Circular; D007375:Interleukin-1; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes