Suicide vector for transposon mutagenesis in Pseudomonas solanacearum.
A suicide vector was constructed by cloning the transfer genes of the wide-host-range (IncW group) plasmid R388 into the BamHI site of pBR325. This plasmid can deliver Tn5 into Pseudomonas solanacearum at frequencies ranging from 10(-6) to 10(-9) per recipient.
Morales
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VM
Sequeira
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D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D005822:Genetic Vectors; D009154:Mutation; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes