Detection of human polyomavirus DNA in urine specimens by hybridot assay.
A hybridot assay method using a labelled BK virus probe has been used to detect the presence of human polyomavirus DNA in 81 urine specimens from 61 patients, most of whom were immuno-compromised to some degree. Twenty-eight urines from 23 patients had detectable DNA. The results have been compared to virus isolation and electron microscopy on the same specimens. In 90 per cent a comparable result was obtained by at least one of the other two tests and in the 73 specimens on which all 3 tests were performed there was 80 per cent agreement between all. In 6 cases the hybridot assay was more sensitive in detecting infection.
Gibson
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PE
Gardner
S D
SD
Porter
A A
AA
D000818:Animals; D001739:BK Virus / Q000302:isolation & purification; D004279:DNA, Viral / Q000652:urine*; D006801:Humans; D007577:JC Virus / Q000302:isolation & purification; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000302:isolation & purification; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine; D014556:Urine / Q000382:microbiology*
D004279:DNA, Viral