Chimeric IgG-binding receptors engineered from staphylococcal protein A and streptococcal protein G.
Chimeric Fc receptors, consisting of the IgG-binding domains of both staphylococcal protein A and streptococcal protein G, were constructed. An efficient bacterial expression system was used to produce the recombinant proteins, which vary in size and number of IgG-binding domains. The purified receptors were analyzed by immunodiffusion and a competitive enzyme-linked immunosorbent assay to establish the relative binding strength to various polyclonal and monoclonal immunoglobulins from different species. The results demonstrate that protein A and protein G have complementary binding patterns and that the chimeric receptors retain the binding capacities of both the parental constituents. This suggests that these novel chimeric receptors might be versatile reagents for immunochemical assays.
Eliasson
M
M
Department of Biochemistry, Royal Institute of Technology, Stockholm, Sweden.
Olsson
A
A
Palmcrantz
E
E
Wiberg
K
K
Inganäs
M
M
Guss
B
B
Lindberg
M
M
Uhlén
M
M
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002678:Chimera; D004285:Dogs; D006168:Guinea Pigs; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000378:metabolism; D051381:Rats; D011961:Receptors, Fc / Q000235:genetics* / Q000378:metabolism; D017452:Receptors, IgG; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics*; D012756:Sheep; D013205:Staphylococcal Protein A / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A