Regulation of single and multicopy his operons in Escherichia coli.
We fused segments of the Escherichia coli his regulatory region to galK in single-copy and multicopy vectors. These fusions demonstrated that (i) derepression of his by histidine starvation is due exclusively to attenuation; (ii) the his promoter is metabolically regulated; and (iii) both regulatory systems operate when the his regulatory region is present on a multicopy plasmid. Thus, there is no evidence for titration of his regulatory elements. Deletions of the his anti-attenuator region, carried on multicopy plasmids, cause low-level galK expression. This expression is not stimulated by histidine starvation, but is growth rate dependent. We replaced the his attenuator with the efficient lambda terminator, to. In the context of the his regulatory region, however, lambda to only partially terminates transcription.
Riccio
A
A
Bruni
C B
CB
Rosenberg
M
M
Gottesman
M
M
McKenney
K
K
Blasi
F
F
D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002872:Chromosome Deletion; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005686:Galactokinase / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006639:Histidine / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009876:Operon; D010957:Plasmids; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D006639:Histidine; D005686:Galactokinase; D004262:DNA Restriction Enzymes