Localization of the upstream regulatory sites of yeast iso2-cytochrome c gene.
In order to study the regulation of expression of the iso2-cytochrome c gene, we have constructed a fused gene between the 5'flanking region of the gene coding for the yeast iso2-cytochrome c and the coding region of the E. coli beta-galactosidase lacZ gene. When introduced in yeast cells this hybrid gene is expressed and regulated like the production of iso2-cytochrome c: it is under the control of the general catabolic repression and of the unlinked trans-acting CYP1 gene whose CYP1-18 allele causes an overproduction of iso2-cytochrome c. The expression of hybrid genes whose upstream region has been progressively shortened or altered by internal deletions was studied either in wild-type CYP1+ cells or in cells carrying the CYP1-18 allele grown either on glucose or on glycerol. It appears that the expression and the regulation of the iso2-cytochrome c gene is controlled by an upstream regulatory site composed of a positive and a negative element. This site is the target of regulation by the CYP1 gene product and, directly or through this gene, of the control by the general catabolic repression.
Iborra
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Francingues
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Guerineau
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D001483:Base Sequence; D002874:Chromosome Mapping; D003574:Cytochrome c Group / Q000031:analogs & derivatives* / Q000235:genetics; D045304:Cytochromes c; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005796:Genes; D005809:Genes, Regulator; D008040:Genetic Linkage; D005947:Glucose / Q000235:genetics; D005990:Glycerol / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D003574:Cytochrome c Group; D004274:DNA, Recombinant; C032139:iso-2-cytochrome C; D045304:Cytochromes c; D001616:beta-Galactosidase; D005947:Glucose; D005990:Glycerol