Degradation of the labelled beta-endorphin is an important factor of direct RIA in the human and rat plasma.
Degradation of iodinated beta-endorphin in human and rat plasma was investigated, using different proteinase inhibitors. This degradation affects the radioimmunoassay quality. Additional blank tubes were also prepared during assay procedure to avoid the effect of 125-I-beta-endorphin adsorption on the dextran-coated charcoal in the presence and absence of plasma proteins, what avoids underestimation of the beta-endorphin level in plasma samples. Based on the above observations, the sensitive and direct radioimmunoassay of beta-endorphin was developed in human and rat plasma.
Silberring
J
J
Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland.
Majeed
N H
NH
Lasoń
W
W
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011485:Protein Binding; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D001615:beta-Endorphin / Q000097:blood* / Q000276:immunology / Q000378:metabolism
D001798:Blood Proteins; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D001615:beta-Endorphin