title: Paramagnetic probes of multicomponent electron-transfer systems.
abstract: Electron-transfer systems associated with steroid hormone production in the adrenals and with photosynthetic NADP+ reduction in the chloroplast share some important features. Complexes between components in both systems affect the exposure of prosthetic groups to water-soluble paramagnetic groups. The sensitivity of the components to probes of different charges suggests that electrostatic interactions are important in complex formation. Haem plane orientation in cytochrome P-450 of the adrenal system is parallel to the membrane plane in both native and reconstituted systems.
authors.forename: H
authors.initials: H
authors.lastname: Kamin
authors.forename: C
authors.initials: C
authors.lastname: Batie
authors.forename: J D
authors.initials: JD
authors.lastname: Lambeth
authors.forename: J
authors.initials: J
authors.lastname: Lancaster
authors.forename: L
authors.initials: L
authors.lastname: Graham
authors.forename: J C
authors.initials: JC
authors.lastname: Salerno
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004579:Electron Transport; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism; D005288:Ferredoxins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism
chemical_list: D005288:Ferredoxins; D003577:Cytochrome P-450 Enzyme System; D004492:Edetic Acid; D013250:Steroid Hydroxylases; D005287:Ferredoxin-NADP Reductase