The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus.
An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences.
Binns
M M
MM
Boursnell
M E
ME
Foulds
I J
IJ
Brown
T D
TD
D003001:Cloning, Molecular; D003332:Coronaviridae / Q000235:genetics*; D004247:DNA / Q000096:biosynthesis*; D005814:Genes, Viral; D001351:Infectious bronchitis virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000096:biosynthesis; D010957:Plasmids
D009838:Oligodeoxyribonucleotides; D004247:DNA