Evaluation of two commercial kits for rapid detection of human rotavirus in feces: Rotalex, a latex agglutination test and Rotavirus ELISA Kit.
96 fecal specimens from children with symptoms of gastroenteritis were tested with a latex agglutination test (Rotalex, Orion Diagnostica, Finland) and an enzyme-linked immunosorbent test (Rotavirus ELISA Kit, Dakopatts, Copenhagen, Denmark). Using the ELISA method as reference, the latex agglutination (LX) came out with three non-specific and two false negative results. The LX test was found to be a rapid, practical and non-expensive choice for a laboratory examinating few samples daily.
Jensen
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D000372:Agglutination Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D006801:Humans; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis*