Subtyping of phosphoglucomutase 1 (PGM1) in microstains of different biological materials with subsequent species differentiation without further stain material consumption.
Micromethods for subtyping of phosphoglucomutase 1 (PGM 1) in small amounts of biological stain material are described, using an applicator for highly diluted stain extracts. With the aid of this applicator strip blood and semen micro-stains as well as single hair-roots could be extracted by electroelution directly on the PGM1 isoelectric focusing gel. Species differentiation was also possible either by radial immunodiffusion using the extract remaining in the applicator strip after isoelectric focusing or by interpretation of the PGM pattern itself.
Pflug
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Kriminaltechnisches Institut, Landeskriminalamt Baden-Württemberg, Stuttgart, Federal Republic of Germany.
D001801:Blood Stains; D005554:Forensic Medicine; D005782:Gels; D006197:Hair / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D010733:Phosphoglucomutase / Q000032:analysis / Q000235:genetics*; D012661:Semen / Q000032:analysis; D013045:Species Specificity
D005782:Gels; D007527:Isoenzymes; D010733:Phosphoglucomutase