S13, a rapidly oncogenic replication-defective avian retrovirus.
The avian leukemia sarcoma virus S13 transforms chicken and Japanese quail embryo fibroblasts and chicken erythroid cells in tissue culture. S13-induced erythroid transformation requires culture conditions suitable for the growth of normal erythroid precursors (H. Beug and M. J. Hayman (1984), Cell 36, 963-972). S13-transformed erythroid colonies contain a high percentage of cells that differentiate in absence of erythropoietin. S13 is defective in pol and env functions but can code for a complete set of gag proteins. Nonproducer cell clones transformed by S13 release a noninfectious viral particle containing gag but no functional env or pol proteins. They also synthesize a transformation-specific protein of 155,000 molecular weight. This protein reacts with antibody to viral envelope glycoproteins and appears to represent onc as well as env sequences. The 155,000-molecular weight env-linked protein does not cross react immunologically with an antiserum against the v-erb A and v-erb B gene products.
Beug
H
H
Hayman
M J
MJ
Graf
T
T
Benedict
S H
SH
Wallbank
A M
AM
Vogt
P K
PK
D001355:Alpharetrovirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000096:biosynthesis / Q000235:genetics; D001854:Bone Marrow Cells; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D003370:Coturnix; D003673:Defective Viruses / Q000502:physiology*; D004900:Erythroblasts; D004920:Erythropoiesis; D005347:Fibroblasts; D006378:Helper Viruses / Q000502:physiology; D009857:Oncogenes; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014779:Virus Replication
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D014764:Viral Proteins