title: In vitro culture of two populations (dividing and nondividing) of exoerythrocytic parasites of Plasmodium vivax.
abstract: Plasmodium vivax sporozoites invaded human hepatoma cells and differentiated into 2 types of exoerythrocytic (EE) parasites. One group was composed of actively dividing schizonts, which released merozoites after 9 days of culture. The second group was nondividing and persisted after the primary schizonts disappeared from the culture. EE schizonts progressively lost reactivity to monoclonal antibodies to the surface-protective protein antigen of P. vivax sporozoites, but the persisting parasites remained strongly reactive. In the 2 strains of P. vivax studied, the ratio of schizonts to persisting parasites was approximately equal.
authors.forename: M R
authors.initials: MR
authors.lastname: Hollingdale
authors.forename: W E
authors.initials: WE
authors.lastname: Collins
authors.forename: C C
authors.initials: CC
authors.lastname: Campbell
authors.forename: A L
authors.initials: AL
authors.lastname: Schwartz
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis; D001399:Azure Stains; D006528:Carcinoma, Hepatocellular; D002455:Cell Division; D002460:Cell Line; D006801:Humans; D008113:Liver Neoplasms; D010966:Plasmodium vivax / Q000166:cytology / Q000254:growth & development* / Q000276:immunology; D014617:Vacuoles / Q000469:parasitology
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D001399:Azure Stains