[The appearance of specific IgG and IgA antibodies to Streptococcus pneumoniae in serum and lung lavage fluid of mice after the administration of different antigens. 2. IgG and IgA antibody detection after intraperitoneal, aerosol and oral immunization with Streptococcus pneumoniae].
The stimulation of secretory and systemic IgG- and IgA-antibodies in the lung lavage fluid was examined in an animal test. It was also examined in the serum of mice after once to four times oral, aerosol or intraperitoneal immunization with total formalin destroyed bacteria of the species Streptococcus pneumoniae type I. Not only in the lung lavage fluid, but also in the sera specific antibodies could be found with the enzyme immunoassay. An intraperitoneal antigen application mainly resulted in increase of the specific IgG in the serum, whereas an oral and especially aerosol antigen application mainly developed a stimulation of IgA in the lung lavage fluid.
Schumann
V
V
Innere Abteilung I des St. Hedwig-Krankenhauses Berlin, DDR.
Tischner
H
H
Noack
K
K
Schröder
R
R
Pohl
W D
WD
D000284:Administration, Oral; D000336:Aerosols; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000008:administration & dosage*; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis; D005260:Female; D007114:Immunization; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis* / Q000096:biosynthesis; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000032:analysis* / Q000096:biosynthesis; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D013047:Specific Pathogen-Free Organisms; D013296:Streptococcus pneumoniae / Q000276:immunology*
D000336:Aerosols; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G
Auftreten von spezifischen IgG- und IgA-Antikörpern gegen Streptococcus pneumoniae im Serum und in der Lungenspülflüssigkeit von Mäusen nach unterschiedlicher Antigenapplikation. 2. Mitteilung: IgG- und IgA-Antikörpernachweis nach intraperitonealer, aerogener und oraler Immunisierung mit Streptococcus pneumoniae.