Nuclear ADP-ribosyl transferase activity correlates with induction of P-450 monooxygenases by phenobarbital in rat liver microsomes.
The effect of phenobarbital treatment on the nuclear ADP-ribosyl transferase activity has been studied in parallel with microsomal cytochrome P-450 concentration and related mono-oxygenase activities, in rat liver. A marked activation of the ADP-ribosyl transferase was observed 24 h after phenobarbital administration. The chronological study performed between 0-6 days after phenobarbital treatment showed a sharp increase in this nuclear enzyme activity, to approximately equal to 270% of the control value produced in 48 h. The administration of 5'-methylnicotinamide in vivo, an inhibitor of ADP-ribosyl transferase activity in vitro, produced a decrease both of the induction of liver microsomal cytochrome P-450 mono-oxygenases and nuclear ADP-ribosyl transferase activity. The role of nuclear ADP-ribosyl transferase in the adaptative response of the liver cell to phenobarbital is discussed.
Lechner
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Bráz
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D000633:Aminopyrine N-Demethylase / Q000096:biosynthesis; D000815:Aniline Hydroxylase / Q000096:biosynthesis; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009536:Niacinamide / Q000031:analogs & derivatives / Q000494:pharmacology; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010105:Oxygenases / Q000096:biosynthesis*; D010634:Phenobarbital / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases; D051381:Rats; D011919:Rats, Inbred Strains
D009536:Niacinamide; C024785:5-methylnicotinamide; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase; D011065:Poly(ADP-ribose) Polymerases; D009713:Nucleotidyltransferases; D010634:Phenobarbital