MZ15, a monoclonal antibody recognizing keratan sulphate, stains chick tendon.
Biochemical, morphometric and electron histochemical methods have failed to demonstrate the presence in tendon of keratan sulphate, a common component of connective tissue proteoglycans. Using a monoclonal antibody specific for keratan sulphate, a positive localization of this molecule was made in the gastrocnemius tendon of stage 44 chicken embryos both at the light and electron microscopical levels.
Craig
F M
FM
Institute of Orthopaedics, Royal National Orthopaedic Hospital, Stanmore, Middlesex, UK.
Ralphs
J R
JR
Bentley
G
G
Archer
C W
CW
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002642:Chick Embryo; D006025:Glycosaminoglycans / Q000032:analysis*; D007150:Immunohistochemistry; D007632:Keratan Sulfate / Q000032:analysis* / Q000276:immunology; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D013710:Tendons / Q000032:analysis* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D006025:Glycosaminoglycans; D007632:Keratan Sulfate