Mouse alpha-amylase loci, Amy-1a and Amy-2a, are closely linked.
We have cloned a contiguous 106 X 10(3) base-pair long stretch of mouse DNA. The isolated chromosomal DNA segment contains the single copy gene Amy-1a that is strongly expressed in the parotid gland and, 23 X 10(3) base-pairs downstream from it, one member of the pancreas-specific Amy-2a oligogene family. At least two of the four Amy-2a genes, including the copy linked to Amy-1a, are efficiently transcribed. The cloned DNA sequences do not appear to specify messenger RNAs other than those encoding alpha-amylase in pancreas, parotid gland or liver. Transcription termination on Amy-1a occurs within 3 X 10(3) base-pairs downstream from the polyadenylation site in both parotid gland and liver, in which this gene is transcribed at different rates from different promoters.
Pittet
A C
AC
Schibler
U
U
D000818:Animals; D001483:Base Sequence; D004247:DNA; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel; D005796:Genes; D008040:Genetic Linkage; D051379:Mice; D010179:Pancreas / Q000201:enzymology; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics*
D004274:DNA, Recombinant; D004247:DNA; D004262:DNA Restriction Enzymes; D000516:alpha-Amylases