Production of human bone marrow-derived suppressor factor. Effect on antibody synthesis and lectin-activated cell proliferation.
Natural suppressor cells resident in normal human bone marrow (BM) exert potent suppressor activity on in vitro antibody responses and other immune functions. A suppressor-enriched population of BM cells can constitutively produce a soluble mediator with similar suppressor activity and kinetics as the suppressor cells. This novel BM-derived suppressor factor (BDSF) suppresses human in vitro primary antibody responses as well as lectin-activated proliferative responses. The mediator (BDSF) has a Mr of less than 1.5 kDa, contains a lipid component, and is insensitive to indomethacin treatment. The BM cells producing the factor bear the HNK-1 surface marker but not T, B, or macrophage markers. The ability of BDSF to suppress Ag-dependent IgM responses during the inductive phase makes it an ideal molecule with the potential to regulate early immune and hemopoietic events within the BM compartment.
Mortari
F
F
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
Singhal
S K
SK
D000911:Antibodies, Monoclonal / Q000633:toxicity; D000917:Antibody Formation / Q000187:drug effects; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism*; D003208:Concanavalin A; D003602:Cytotoxicity, Immunologic; D004247:DNA / Q000096:biosynthesis; D006462:Hemolytic Plaque Technique; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000502:physiology; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis* / Q000302:isolation & purification / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011500:Protein Synthesis Inhibitors; D013491:Suppressor Factors, Immunologic; C035892:soluble immune-response suppressor, bone marrow; D003208:Concanavalin A; D004247:DNA