Collisional spectroscopy as a screening procedure for the determination of FFI in complex natural matrices.
The build-up of brown products on long-lived proteins could be an important factor in determining long-term diabetic complications. A brown product 2-(2-furoyl)-4(5)-(2-furanyl)-1H-imidazole (FFI), present in the acid hydrolysate mixtures of beta-polylysine and beta-albumin has been identified by collisional spectroscopy, using a commercial double-focusing, reverse-geometry mass spectrometer. Using this approach the analyses were carried out without any sample derivatization or need for chromatographic separation procedures.
Poli
T
T
Istituto di Medicina Interna, Patologia Medica I., Policlinico Universitario, Padova, Italy.
Lapolla
A
A
Pelli
B
B
Sturaro
A
A
Traldi
P
P
Crepaldi
G
G
Fedele
D
D
D000418:Albumins / Q000032:analysis; D048909:Diabetes Complications; D003920:Diabetes Mellitus / Q000378:metabolism; D007093:Imidazoles / Q000032:analysis*; D011107:Polylysine / Q000032:analysis; D013057:Spectrum Analysis / Q000379:methods
D000418:Albumins; D007093:Imidazoles; D011107:Polylysine; C041306:2-(2-furoyl)-4(5)-(2-furanyl)-1H-imidazole