title: Detection of sendai virus protein by reversed-phase high-performance liquid chromatography combined with immuno-chromatography.
abstract: Reversed-phase high-performance liquid chromatography (RP-HPLC) was applied to the detection of Sendai virus F2 protein. Immunoaffinity chromatography was used prior to RP-HPLC to increase the specificity. With this combined method, the presence of F2 protein in complex solutions could be demonstrated within 150 min. Using RP-HPLC, F2 protein could be detected in amounts down to 20 ng, which could be reduced to 1 ng by application of a microbore column.
authors.forename: R
authors.initials: R
authors.lastname: van der Zee
authors.forename: G W
authors.initials: GW
authors.lastname: Welling
mesh_terms: D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007120:Immunochemistry; D010222:Parainfluenza Virus 1, Human / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D014764:Viral Proteins