Ouabain-insensitive active sodium transport in rat jejunum: evidence from ATPase activities, Na uptake by basolateral membrane vesicles and in vitro transintestinal transport.
The basolateral membrane of the jejunal enterocyte of the rat was separated by self-orienting Percoll-gradient centrifugation and further purified from brush border contamination. Pellets were analysed for Mg-, Na- and (Na,K)-ATPase activities. The uptake of 0.02 M NaCl was also followed by the rapid micro-filtration technique. Transintestinal transport of fluid and electrolytes, and cell water, Na and K were determined in the in vitro everted and incubated jejunum. There is ouabain-insensitive Na-ATPase in addition to the well-known (Na,K)-ATPase in the basolateral membrane. These are differently inhibited by furosemide and ethacrynate. Na uptake by osmotically active basolateral membrane vesicles is enhanced by ATP and a further enhancement is obtained if there is intravesicular K. The ATP effect is inhibited differently by strophanthidin, furosemide and ethacrynate. In the everted sac preparation, transintestinal transport of Na and fluid still occurs when the Na/K pump is totally inhibited by ouabain. These experimental results suggest that there is also a ouabain-insensitive Na pump, different from the Na/K pump, in the basolateral membrane.
Tosco
M
M
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Orsenigo
M N
MN
Esposito
G
G
Faelli
A
A
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D027682:Cation Transport Proteins; D002462:Cell Membrane / Q000201:enzymology; D066298:In Vitro Techniques; D007583:Jejunum / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D014882:Water-Electrolyte Balance
D027682:Cation Transport Proteins; D010042:Ouabain; D000255:Adenosine Triphosphate; D012964:Sodium; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase