The activity of membranes reconstituted from HVJ envelope proteins and lipids to induce hemolysis and fusion between liposomes and erythrocytes.
A simple method for preparation of lipid-free envelope proteins (HN protein and F protein) of HVJ (Sendai virus) was developed. Reconstituted 'envelopes' were then prepared from envelope proteins and various lipids by the detergent dialysis method, and the activity to induce hemolysis and fusion between liposome and erythrocyte was studied. Lipid-free envelope protein aggregates could induce hemolysis and liposome-erythrocyte fusion. The activity was however greatly augmented by incorporation of envelope proteins into membrane of viral total lipids. Hemolytic and fusogenic activity was somewhat augmented by incorporation of envelope proteins into dipalmitoylphosphatidylcholine/cholesterol (1:1, molar ratio) and dimyristoylphosphatidylcholine/cholesterol (1:1), though the augmentation was lower than that observed with viral total lipids. When 'envelopes' were reconstituted with the proteins and viral total lipids supplemented with phosphatidylethanolamine, two kinds of 'envelopes' were prepared; one was permeable to Dextran (Mr 75000) and hemolytic, and the other was impermeable to Dextran and nonhemolytic. The latter acquired hemolytic activity after subjection to freezing and thawing, and its barrier function was lost concomitantly. The study suggests that envelope proteins (HN protein and F protein) could function without lipids but their activity was greatly influenced by not only the composition of additional lipids but also mode of arrangement of components on the reconstituted membranes.
Inoue
J
J
Nojima
S
S
Inoue
K
K
D002459:Cell Fusion; D002499:Centrifugation, Density Gradient; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine; D004912:Erythrocytes / Q000378:metabolism*; D005615:Freezing; D006461:Hemolysis; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D008722:Methods; D010222:Parainfluenza Virus 1, Human / Q000032:analysis*; D010714:Phosphatidylethanolamines; D011663:Pulmonary Surfactants; D014759:Viral Envelope Proteins / Q000378:metabolism*
D008081:Liposomes; D010714:Phosphatidylethanolamines; D011663:Pulmonary Surfactants; D014759:Viral Envelope Proteins; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine