Identification of the short dispersed repetitive DNA sequences isolated from the zones of initiation of DNA synthesis in human cells as Alu-elements.
DNA of Xeroderma pigmentosum cells was crosslinked in vivo with trioxsalen and long wave length ultraviolet light and the cells were cultured in the presence of labelled thymidine for one hour. The nascent DNA chains synthesized during this period and containing the DNA replication origins were isolated from the high molecular weight chromosomal DNA by an alkaline sucrose density gradient centrifugation. They were 5-10-fold enriched in short dispersed repetitive sequences identified by dot-blot hybridization to BLUR 8 plasmid as members of the human Alu-family.
Anachkova
B
B
Russev
G
G
Altmann
H
H
D004247:DNA / Q000302:isolation & purification*; D004261:DNA Replication; D004720:Endonucleases / Q000378:metabolism; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D012091:Repetitive Sequences, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D012871:Skin Diseases / Q000235:genetics; D014307:Trioxsalen / Q000494:pharmacology; D014983:Xeroderma Pigmentosum / Q000235:genetics
D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014307:Trioxsalen