Antigen presenting cells in human decidual tissue: III. Role of accessory cells in the activation of suppressor cells.
Human decidual antigen presenting cells (DAPCs) exposed to fetal cells in vitro induce generation of suppressor T cells among a population of peripheral blood lymphocytes. Human lymphocyte antigen (HLA)-class II positive antigen presenting cells were isolated from early normal human decidual tissue and from peripheral blood (PAPCs) by adhering Ficoll-Paque separated cell suspensions to fibronectin. In contrast to PAPCs, DAPCs pulsed with fetal antigens induced a radio-sensitive, Leu 1,2-positive T suppressor cell population. A nylon wool adherent B cell population is required during the in vitro induction of the suppressor cells. These suppressor cells impair primary mitogen and mixed lymphocyte culture (MLC) responses, generation of anti-trinitrophenyl (TNP) cytotoxic T lymphocytes, and antibody response of autologous and allogeneic lymphocytes. Only intact viable embryonic cells can effectively confer upon DAPCs the ability to induce T suppressor cells. The T suppressor cell induction by DAPCs primed with fetal antigens is restricted by the major histocompatibility complex. Our results show that the HLA-DR molecules are the most prominent restriction elements.
Oksenberg
J R
JR
Lautenberg Center for Tumor and General Immunology, Hadassah Medical School, Hebrew University of Jerusalem, Israel.
Mor-Yosef
S
S
Ezra
Y
Y
Brautbar
C
C
D000328:Adult; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002448:Cell Adhesion; D003656:Decidua / Q000276:immunology*; D005260:Female; D005333:Fetus / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D011247:Pregnancy; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D006684:HLA-DR Antigens