Induction of suppression of delayed type hypersensitivity to herpes simplex virus by epidermal cells exposed to UV-irradiated urocanic acid in vivo.
Urocanic acid (UCA), the putative photoreceptor for ultraviolet radiation (UV)-induced suppression, undergoes a UV-dependent trans to cis isomerisation. Epidermal cells from mice painted with UCA, containing a known proportion of the cis-isomer, generate suppression of the delayed type hypersensitivity response to herpes simplex virus type 1 (HSV-1) when transferred to naive syngeneic recipients at the same time and site as infection with HSV-1. One T suppressor cell subset, of phenotype (Thy1+, L3T4+, Ly2-), is induced by the cis-UCA modified epidermal cell transfer. Flow cytometric analysis of the epidermal cells from skin treated with UV or cis-UCA indicates an overall reduction from normal in the number of cells expressing MHC Class II antigens, but no alteration in the number expressing I-J antigens.
Ross
J A
JA
Department of Clinical and Laboratory Haematology, Western General Hospital, Edinburgh, Scotland.
Howie
S E
SE
Norval
M
M
Maingay
J P
JP
D000818:Animals; D002469:Cell Separation; D004342:Drug Hypersensitivity / Q000276:immunology; D004817:Epidermis / Q000187:drug effects*; D005434:Flow Cytometry; D006561:Herpes Simplex / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007093:Imidazoles / Q000528:radiation effects*; D007165:Immunosuppression Therapy; D051379:Mice; D008809:Mice, Inbred C3H; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014560:Urocanic Acid / Q000494:pharmacology / Q000528:radiation effects*; D014709:Vero Cells / Q000378:metabolism
D000949:Histocompatibility Antigens Class II; D007093:Imidazoles; D014560:Urocanic Acid