Thymocyte activating factors from SV40-transformed human embryo fibroblasts.
A novel factor was found in the medium conditioned by SV40-transformed human embryo fibroblasts, which stimulate concanavalin A-induced thymocyte DNA synthetic response. This activity was estimated to be 10-15 kD and divided into two activities by ion exchange chromatography. One of them is a protein molecule and the other is a glycoprotein. In addition, these activities are not derived from the growth factors reported previously such as interleukin 2 (Morgan, R., Ruscetti, F. and Gallo, R. C. (1976) Science 193, 1007-1008) and transforming growth factor (De Larco, J. E. and Todaro, G. J. (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 4001-4005).
Okai
Y
Y
Gotoh
S
S
Yamashita
U
U
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004622:Embryo, Mammalian; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000302:isolation & purification*; D008213:Lymphocyte Activation; D051379:Mice; D013539:Simian virus 40; D013601:T-Lymphocytes / Q000276:immunology*
D007375:Interleukin-1; D003208:Concanavalin A