[Heterologous desensitization of pigeon erythrocyte adenylate cyclase by the catalytic subunit of cAMP-dependent protein kinase].
Preincubation of pigeon erythrocyte plasma membranes with the catalytic subunit of cAMP-dependent protein kinase results in the desensitization of erythrocyte adenylate cyclase. The adenylate cyclase activity measured in the presence of 10 microM isoproterenol and 50 microM GTP-gamma-S decreases by 40% after 10 min incubation; that in the presence of 50 microM GTP-gamma-S by 35% (20 min). The decrease of the adenylate cyclase activity is due to the prolongation of the lag phase of the enzyme activation in the presence of a hydrolysis-resistant GTP analog and to the drop in activity in the steady state of the activation. The heterologous desensitization of adenylate cyclase induced by cAMP-dependent protein kinase is also coupled with the decrease of the number of beta-adrenoreceptors capable of acquiring a high affinity for the agonists in the absence of guanyl nucleotides. The effect of the catalytic subunit on adenylate cyclase is fully compatible with the process of the enzyme desensitization in erythrocytes treated with isoproterenol or cAMP.
Popov
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Bilargina
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Severin
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D000262:Adenylyl Cyclases / Q000097:blood* / Q000378:metabolism; D000818:Animals; D010856:Columbidae / Q000097:blood; D004910:Erythrocyte Membrane / Q000201:enzymology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D046911:Macromolecular Substances; D010766:Phosphorylation; D011494:Protein Kinases / Q000097:blood* / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D013873:Thionucleotides / Q000378:metabolism
D046911:Macromolecular Substances; D011943:Receptors, Adrenergic, beta; D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D011494:Protein Kinases; D000262:Adenylyl Cyclases; D007545:Isoproterenol
Geterologicheskaia desensitizatsiia adenilattsiklazy iz éritrotsitov golubia pod deĭstviem kataliticheskoĭ sub"edinitsy cAMP-zavisimoĭ proteinkinazy.