title: The cytoplasmically-made subunit IV is necessary for assembly of cytochrome c oxidase in yeast.
abstract: Yeast cytochrome c oxidase contains three large subunits made in mitochondria and at least six smaller subunits made in the cytoplasm. There is evidence that the catalytic centers (heme a and copper) are associated with the mitochondrially-made subunits, but the role of the cytoplasmically-made subunits has remained open. Using a gene interruption technique, we have now constructed a Saccharomyces cerevisiae mutant which lacks the largest of the cytoplasmically-made subunits (subunit IV). This mutant is devoid of cyanide-sensitive respiration, the absorption spectrum of cytochrome aa3 and cytochrome c oxidase activity. It still contains the other cytochrome c oxidase subunits but these are not assembled into a stable complex. Active cytochrome c oxidase was restored to the mutant by introducing a plasmid-borne wild-type subunit IV gene; no restoration was seen with a gene carrying an internal deletion corresponding to amino acid residues 28-66 of the mature subunit. Subunit IV is thus necessary for proper assembly of cytochrome c oxidase.
authors.forename: W
authors.initials: W
authors.lastname: Dowhan
authors.forename: C R
authors.initials: CR
authors.lastname: Bibus
authors.forename: G
authors.initials: G
authors.lastname: Schatz
mesh_terms: D003593:Cytoplasm / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005111:Extrachromosomal Inheritance; D005796:Genes; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D003576:Electron Transport Complex IV