Release of IgD-binding factor by T cells under the influence of interleukin 2, interleukin 4, or cross-linked IgD.
Helper T cells with receptors specific for IgD have immunoaugmenting properties. We have now detected soluble IgD-binding factor in cell supernatants immobilized on nitrocellulose paper by their ability to bind 125I-labeled IgD. IgD-binding factor is released by normal splenic T cells stimulated with recombinant interleukin 2, recombinant interleukin 4, or crosslinked IgD in amounts paralleling the induction of IgD receptors on the cells. IgD receptors are constitutively produced by antigen-specific helper T-cell hybridomas 2H10 and A3.4C6. Incubation of these hybridoma cells with recombinant interleukin 2 increases release of IgD-binding factor while reducing expression of IgD receptors. Specificity of the binding factor for IgD is established by (i) competitive inhibition; (ii) the ability of the binding factor to bind radiolabeled IgD and not monoclonal IgE, IgG2a, or polyclonal IgG; and (iii) the removal of the binding factor on passage through an IgD-Sepharose column and recovery in a subsequent acid eluate.
Amin
A R
AR
Department of Pathology, New York University Medical Center, NY 10016.
Coico
R F
RF
Finkelman
F
F
Siskind
G W
GW
Thorbecke
G J
GJ
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D007072:Immunoglobulin D / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology*; D015847:Interleukin-4; D007378:Interleukins / Q000276:immunology*; D008222:Lymphokines / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D029584:Prostatic Secretory Proteins; D011994:Recombinant Proteins / Q000276:immunology*; D013491:Suppressor Factors, Immunologic / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D007072:Immunoglobulin D; D007376:Interleukin-2; D007378:Interleukins; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D011994:Recombinant Proteins; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D015847:Interleukin-4