Purification of Plasmodium falciparum digestive vacuoles and partial characterization of the vacuolar membrane ATPase.
Plasmodium falciparum digestive vacuoles containing ferric oxide granules were purified from parasite homogenates by centrifugation on discontinuous sucrose gradients. Digestive vacuole membranes prepared by osmotic lysis and washed with KCl showed no detectable contamination by erythrocyte membrane proteins and only minimal contamination by non-vacuolar parasite proteins. Purified vacuolar membranes were 2.6-fold enriched in total parasite membrane ATPase activity. This ATPase was optimally active at pH 7 in the presence of at least 2 mM Mg2+. Ca2+ and Mn2+ were approximately 80-90% as effective as Mg2+, and Zn2+, Co2+ and Fe2+ also exerted some stimulatory effect. The vacuolar membrane also hydrolyzed GTP, UTP, CTP and ADP, but AMP and 3',5'-cyclic AMP were hydrolyzed only one-tenth as effectively as ATP. The ATPase was unaffected by vanadate, ouabain or oligomycin but was significantly inhibited by the proton pump inhibitors NEM and NBD-Cl. Of 6 antimalarial drugs tested, quinine and quinacrine were the most effective inhibitors and mefloquine was the least effective.
Choi
I
I
Department of Biology, University of Alabama, Tuscaloosa 35487.
Mego
J L
JL
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005290:Ferric Compounds; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000201:enzymology; D010963:Plasmodium falciparum / Q000201:enzymology*; D014617:Vacuoles / Q000201:enzymology*
D000962:Antimalarials; D002413:Cations, Divalent; D005290:Ferric Compounds; C000499:ferric oxide; D000251:Adenosine Triphosphatases