title: Chromosomal localization and preliminary characterization of the human gene encoding insulin-like growth factor II.
abstract: A cDNA probe corresponding to mRNA encoding a closely related variant of human insulin-like growth factor II (IGF-II) was used for the chromosomal assignment of the IGF-II gene. Southern blot hybridization analysis of DNA from human-Chinese hamster somatic cell hybrids showed that the human IGF-II gene is located on chromosome 11. Using the same IGF-II variant probe, a cosmid was isolated which contains the human IGF-II gene. Restriction enzyme analysis revealed that the gene encoding IGF-II has a discontinuous structure and contains at least four exons.
authors.forename: P
authors.initials: P
authors.lastname: de Pagter-Holthuizen
authors.forename: J W
authors.initials: JW
authors.lastname: Höppener
authors.forename: M
authors.initials: M
authors.lastname: Jansen
authors.forename: A H
authors.initials: AH
authors.lastname: Geurts van Kessel
authors.forename: G J
authors.initials: GJ
authors.lastname: van Ommen
authors.forename: J S
authors.initials: JS
authors.lastname: Sussenbach
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D007328:Insulin / Q000235:genetics*; D010455:Peptides / Q000235:genetics*; D010957:Plasmids; D013002:Somatomedins / Q000235:genetics*
chemical_list: D007328:Insulin; D010455:Peptides; D013002:Somatomedins; D004247:DNA; D004262:DNA Restriction Enzymes