U5 snRNP particles bind pre-messenger RNA in vitro.
The ability of purified U5 small nuclear RNP particles (U5 snRNPs) to bind in vitro pre-mRNA of the human beta-globin gene was investigated. The transcript which contained sequences, corresponding to the second intron and fragments of the second and third exons of the gene was bound to U5 snRNP particles in the presence of antibodies specific against U5 snRNPs. The in vitro splicing of pre-mRNA was inhibited in the presence of autoantibodies against U5 snRNP particles. The results are discussed in terms of the possible role of U5 RNP particles in pre-mRNA splicing.
Pironcheva
G L
GL
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Russev
G
G
D000906:Antibodies / Q000276:immunology; D002460:Cell Line; D005914:Globins / Q000032:analysis / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D011485:Protein Binding; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000276:immunology / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear
D000906:Antibodies; D012322:RNA Precursors; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D005914:Globins