Prevention of supersensitivity-like phenomena in rat vas deferens by colchicine.
Rat vas deferens has been shown to become supersensitive to alpha-adrenergic agonists on brief treatment with epinephrine (Epi-treatment). Epi-treatment increased the contractile response and the number of alpha-adrenoceptors in the tissue. The application of colchicine (2.5 mM) during Epi-treatment prevented the supersensitivity-like phenomena and increase in number of alpha-adrenoceptors. Vinblastine (10 microM) also counteracted the effect of Epi-treatment but cytochalasin B (100 microM) and strychnine (2.5 mM) did not. It is suggested that the supersensitivity-like phenomena induced in rat vas deferens by Epi-treatment were due to a change in microtubular components of the membrane.
Hata
F
F
Noguchi
Y
Y
Koda
N
N
Kihira
Y
Y
Kondo
E
E
Ishikawa
Y
Y
Ishida
H
H
D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008870:Microtubules / Q000502:physiology; D009119:Muscle Contraction / Q000187:drug effects; D011224:Prazosin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects; D014649:Vas Deferens / Q000187:drug effects*
D011942:Receptors, Adrenergic, alpha; D003078:Colchicine; D011224:Prazosin; D004837:Epinephrine