Intraparenchymal allografts in the mouse brain in relation to immunocytochemical identification of T lymphocyte subsets.
In a study of intracerebellar allografts of mice brainstem anlagen (embryonic day 12-14), we examined immunocytochemically the expression of two different types of T lymphocytes in and around the grafts. Helper/inducer and cytotoxic/suppressor T cells were identified with anti-L3T4 and anti-Lyt-2 monoclonal antibodies, respectively. Allografts into major histocompatibility complex (MHC)-compatible recipients showed no histological signs of rejection such as marked neovascularization and cellular infiltrates even 6 months after transplantation, but those into MHC-incompatible recipients generally had rejection reactions within one month after transplantation. In the latter cases, the L3T4/Lyt-2 ratio for the T lymphocytes in the infiltrates of the grafts was 1.03 +/- 0.14 (mean +/- S.D.), suggesting that both helper/inducer and cytotoxic/suppressor T cells may play important roles in the mediation of intraparenchymal brain allograft rejection.
Date
I
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Department of Neurological Surgery, Okayama University Medical School, Japan.
Kawamura
K
K
Nakashima
H
H
Ono
K
K
Nishimoto
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D001933:Brain Stem / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D002531:Cerebellum / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D006084:Graft Rejection; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000502:physiology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal