title: Linear simian virus 40 DNA fragments exhibit a propensity for rolling-circle replication.
abstract: A linear simian virus 40 origin-containing DNA fragment replicated in monkey COS cells, generating tandemly repeated (head-to-tail) structures. Electron microscopy revealed circle-and-tail configurations characteristic of rolling-circle replication intermediates. Circularization of the same DNA before transfection led to a theta type of replication which generated supercoiled DNA molecules.
authors.forename: I
authors.initials: I
authors.lastname: Deichaite
authors.forename: Z
authors.initials: Z
authors.lastname: Laver-Rudich
authors.forename: D
authors.initials: D
authors.lastname: Dorsett
authors.forename: E
authors.initials: E
authors.lastname: Winocour
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004261:DNA Replication; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D008854:Microscopy, Electron; D013539:Simian virus 40 / Q000235:genetics*
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral