Biochemical and physiochemical characteristics of a pre-B cell stimulating factor produced by the plasmacytoma cell line LICR LON HMY2.
We have characterized the pre-B cell colony stimulating activity (pre-B cell CSA) from LICR LON HMY2 conditioned medium (CM) by a variety of biochemical techniques. Pre-B cell CSA was found to be associated with a heat stable glycoprotein which has an isoelectric point of 8.3 and a mol. wt, as determined by polyacrylamide gel electrophoresis, of 28-32 kD. The relationship of this activity to previously described factors acting on cells of the B cell lineage is discussed.
Trudgett
A
A
Fay
A C
AC
Thompson
J M
JM
McNeill
T A
TA
D001402:B-Lymphocytes; D002460:Cell Line; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006867:Hydrolases; D007526:Isoelectric Point; D008970:Molecular Weight; D010504:Periodic Acid / Q000494:pharmacology; D010954:Plasmacytoma / Q000378:metabolism*; D017680:Potassium Compounds; D013234:Stem Cells
D003115:Colony-Stimulating Factors; D017680:Potassium Compounds; D010504:Periodic Acid; D006867:Hydrolases; C037192:potassium periodate