title: Amino-acid substitutions at codon 13 of the N-ras oncogene in human acute myeloid leukaemia.
abstract: DNAs from four out of five patients with acute myeloid leukaemia (AML) tested by an in vivo selection assay in nude mice using transfected mouse NIH 3T3 cells were found to contain an activated N-ras oncogene. Using a set of synthetic oligonucleotide probes, we have detected a mutation at codon 13 in all four genes. The same codon is mutated in an additional AML DNA that is positive in the focus-formation assay on 3T3 cells. DNA from the peripheral blood of one patient in remission does not contain a codon 13 mutation.
authors.forename: J L
authors.initials: JL
authors.lastname: Bos
authors.forename: D
authors.initials: D
authors.lastname: Toksoz
authors.forename: C J
authors.initials: CJ
authors.lastname: Marshall
authors.forename: M
authors.initials: M
authors.lastname: Verlaan-de Vries
authors.forename: G H
authors.initials: GH
authors.lastname: Veeneman
authors.forename: A J
authors.initials: AJ
authors.lastname: van der Eb
authors.forename: J H
authors.initials: JH
authors.lastname: van Boom
authors.forename: J W
authors.initials: JW
authors.lastname: Janssen
authors.forename: A C
authors.initials: AC
authors.lastname: Steenvoorden
mesh_terms: D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000235:genetics; D001665:Binding Sites; D004262:DNA Restriction Enzymes; D006160:Guanosine Triphosphate; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D009154:Mutation; D009857:Oncogenes; D011110:Polymorphism, Genetic; D014162:Transfection; D014633:Valine / Q000235:genetics
chemical_list: D001224:Aspartic Acid; D006160:Guanosine Triphosphate; D004262:DNA Restriction Enzymes; D014633:Valine