title: A rapid and sensitive enzyme immunoassay for Cu/Zn superoxide dismutase with polyclonal and monoclonal antibodies.
abstract: An enzyme immunoassay for the quantification of human Cu/Zn SOD in serum, urine and erythrocytes was developed applying monoclonal and polyclonal antibodies. The one-step assay is completed within 30 min and enables the detection of 0.3 microgram Cu/Zn SOD per litre. A Cu/Zn SOD concentration of 46 +/- 21.5 micrograms/l and of 1 +/- 0.6 micrograms/mmol creatinine was determined in the serum and the urine, respectively, of healthy individuals. A content of 15 +/- 1.7 ng Cu/Zn SOD was found in 10(6) erythrocytes. Patients with Down's syndrome exhibited a 3.8-fold, a 2-fold and a 1.6-fold higher concentration of Cu/Zn SOD in their serum, urine and erythrocytes.
authors.affiliation: Institute of Medical Immunology, Faculty of Medicine, Humboldt University of Berlin, GDR.
authors.forename: T
authors.initials: T
authors.lastname: Porstmann
authors.forename: R
authors.initials: R
authors.lastname: Wietschke
authors.forename: H
authors.initials: H
authors.lastname: Schmechta
authors.forename: R
authors.initials: R
authors.lastname: Grunow
authors.forename: B
authors.initials: B
authors.lastname: Porstmann
authors.forename: R
authors.initials: R
authors.lastname: Bleiber
authors.forename: M
authors.initials: M
authors.lastname: Pergande
authors.forename: S
authors.initials: S
authors.lastname: Stachat
authors.forename: R
authors.initials: R
authors.lastname: von Baehr
mesh_terms: D000906:Antibodies; D000911:Antibodies, Monoclonal; D002648:Child; D004314:Down Syndrome / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques; D013482:Superoxide Dismutase / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D013482:Superoxide Dismutase