title: Antibodies against a synthetic peptide as a probe for the kinase activity of the avian EGF receptor and v-erbB protein.
abstract: The transforming protein v-erbB of avian erythroblastosis virus (AEV) displays extensive sequence homology with the presumptive protein-tyrosine kinase domain of the human EGF receptor and with the src protein-tyrosine kinase family of oncogenes. However, no kinase activity has previously been demonstrated for the v-erbB protein. Here antibodies generated against a synthetic peptide from the C terminus of human EGF receptor are shown to immunoprecipitate the EGF receptor from human and avian cells, as well as the v-erbB proteins from AEV-transformed cells that become phosphorylated on tyrosine residues upon the addition of gamma-32P-ATP. The immunoprecipitates are also able to phosphorylate exogenous tyrosine-containing substrates. Hence, it is likely that both avian EGF receptor and v-erbB proteins are protein tyrosine-specific protein kinases. Since the kinase activity of v-erbB protein cannot be regulated by EGF, it is proposed that the tyrosine protein kinase function of v-erbB may be constitutively activated.
authors.forename: R M
authors.initials: RM
authors.lastname: Kris
authors.forename: I
authors.initials: I
authors.lastname: Lax
authors.forename: W
authors.initials: W
authors.lastname: Gullick
authors.forename: M D
authors.initials: MD
authors.lastname: Waterfield
authors.forename: A
authors.initials: A
authors.lastname: Ullrich
authors.forename: M
authors.initials: M
authors.lastname: Fridkin
authors.forename: J
authors.initials: J
authors.lastname: Schlessinger
mesh_terms: D001355:Alpharetrovirus / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D001354:Avian Leukosis Virus / Q000201:enzymology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D066246:ErbB Receptors; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D009857:Oncogenes; D010446:Peptide Fragments / Q000276:immunology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000276:immunology / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D000941:Antigens; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D014764:Viral Proteins; D011494:Protein Kinases; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases