title: Construction of a vector, pRSVcatamb38, for the rapid and sensitive assay of amber suppression in human and other mammalian cells.
abstract: We describe the generation of an amber mutation in the chloramphenicol acetyltransferase (cat) gene of the mammalian cell transfection vector pRSVcat (Gorman et.al. (1982), Proc.Natl.Acad.Sci. 79 6777-6791). We have demonstrated the in vivo suppression of this amber mutation in monkey and human cells by co-transfection with a synthetic Xenopus suppressor tRNATyr under the control of the late SV40 promoter. The vector, pRSVcatamb38, may be used to quantitate amber suppression in various mammalian cells.
authors.forename: J F
authors.initials: JF
authors.lastname: Burke
authors.forename: A E
authors.initials: AE
authors.lastname: Mogg
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes; D005818:Genetic Engineering; D005822:Genetic Vectors; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D012343:RNA, Transfer / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: D012343:RNA, Transfer; D000123:Acetyltransferases; D015500:Chloramphenicol O-Acetyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D004262:DNA Restriction Enzymes