Relaxation of PvuII recognition sequence.
The substrate specificity of PvuII endonuclease is relaxed in the presence of dimethyl sulfoxide. The new recognition sequences cleaved in pBR322 DNA have been found to be CCGCTG, CATCTG, CAGATG, CAGGTG and CAGCGG.
Nasri
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Sayadi
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Thomas
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D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D004270:DNA, Circular / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D010957:Plasmids; D013379:Substrate Specificity
D004269:DNA, Bacterial; D004270:DNA, Circular; D004262:DNA Restriction Enzymes; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D004121:Dimethyl Sulfoxide