Immunochemical characterization and quantitation of lipoprotein (a) in baboons. Development of an assay depending on two antigenically distinct proteins.
We have raised specific antibodies against the protein component of baboon lipoprotein (a) (Lp(a]. Apolipoprotein (apo) Lp(a) is a very large protein which separates into two distinct proteins, apo B and apo (a), when 2-mercaptoethanol is included during sample treatment for sodium dodecyl sulfate-electrophoresis. The antibodies were specific for baboon apo (a) and apo B. The presence of the two distinct antigens in the lipoprotein permitted the development of an enzyme-linked immunosorbent assay that was specific for Lp(a) particles in serum. The assay could detect less than 1 ng of Lp(a) protein per well and was useful in the range of 1-9 ng. The assay was specific for Lp(a) and did not respond to other lipoproteins, such as low density lipoprotein. Lp(a) could be accurately quantitated in serum frozen at -80 degrees C in plastic tubing segments. Using the Lp(a) assay, the mean serum level of 80 unrelated baboons was 4.7 mg/dl, with the distribution skewed toward the lower levels. These data further support the value of the baboon as a model of the atherogenic lipoprotein Lp(a).
Rainwater
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Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78284.
Manis
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D000818:Animals; D000918:Antibody Specificity; D001054:Apolipoproteins A / Q000276:immunology; D001055:Apolipoproteins B / Q000276:immunology; D001797:Blood Protein Electrophoresis; D002499:Centrifugation, Density Gradient; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D017270:Lipoprotein(a); D008074:Lipoproteins / Q000097:blood* / Q000276:immunology; D010215:Papio / Q000097:blood*
D001054:Apolipoproteins A; D001055:Apolipoproteins B; D017270:Lipoprotein(a); D008074:Lipoproteins