title: Dot-blot assays and their use as a direct antigen-binding method to screen monoclonal antibodies to 1,4-beta- and 1,3-beta-glucan synthases.
abstract: A rapid method has been developed to assay beta-glucan synthases spotted on a nitrocellulose sheet. The sensitivity of this method allows screening of hybridoma-making monoclonal antibodies in a direct antigen-binding assay by measurement of the activity of the enzymes retained by the antibodies previously fixed on nitrocellulose.
authors.affiliation: Laboratoire de Différenciation Fongique, UA CNRS 1127, Université Claude Bernard Lyon, Villeurbanne, France.
authors.forename: P
authors.initials: P
authors.lastname: Nodet
authors.forename: J
authors.initials: J
authors.lastname: Grange
authors.forename: M
authors.initials: M
authors.lastname: Fevre
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D029681:Arabidopsis Proteins; D005964:Glucosyltransferases / Q000276:immunology*; D015151:Immunoblotting / Q000379:methods; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009868:Oomycetes / Q000201:enzymology; D029702:Schizosaccharomyces pombe Proteins
chemical_list: D000911:Antibodies, Monoclonal; D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D029702:Schizosaccharomyces pombe Proteins; D005964:Glucosyltransferases; C457769:PRC1 protein, Arabidopsis; C028634:cellulose synthase (UDP-forming); C020570:1,3-beta-glucan synthase