Benzodiazepine receptor binding in vivo with [3H]-Ro 15-1788.
In vivo benzodiazepine receptor binding has generally been studied by "ex vivo" techniques. In this investigation, we identify the conditions where [3H]-Ro 15-1788 labels benzodiazepine receptors by true "in vivo" binding, i.e. where workable specific to nonspecific ratios are obtained in intact tissues without homogenization or washing. [3H]-Flunitrazepam and [3H]-clonazepam did not exhibit useful in vivo receptor binding.
Goeders
N E
NE
Kuhar
M J
MJ
D000818:Animals; D001345:Autoradiography; D001570:Benzodiazepinones / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002998:Clonazepam / Q000378:metabolism; D005442:Flumazenil; D005445:Flunitrazepam / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011963:Receptors, GABA-A / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D001570:Benzodiazepinones; D011963:Receptors, GABA-A; D014316:Tritium; D005442:Flumazenil; D002998:Clonazepam; D005445:Flunitrazepam