Crystallization of and preliminary crystallographic data for Bacillus stearothermophilus cyclodextrin glucanotransferase.
Cyclodextrin glucanotransferase from Bacillus stearothermophilus TC-91 has been crystallized from an ammonium sulfate solution by the dialysis equilibrium method. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with cell dimensions of a = 125.5 A, b = 88.1 A, and c = 81.5 A. The crystals appear to be suitable for X-ray structure analysis, diffracting to at least 2.1 A and being resistant to radiation damage.
Kubota
M
M
Research Institute for Food Science, Kyoto University.
Mikami
B
B
Tsujisaka
Y
Y
Morita
Y
Y
D002852:Chromatography, Ion Exchange; D003460:Crystallization; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D005964:Glucosyltransferases / Q000302:isolation & purification; D011487:Protein Conformation; D014462:Ultrafiltration; D014961:X-Ray Diffraction
D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase