Analysis of deoxynivalenol as its trifluoroacetyl ester by gas chromatography-electron ionization mass spectrometry.
Mass spectra of the trifluoroacetyl esters of the trichothecenes deoxynivalenol, diacetoxyscirpenol and T-2 toxin, as well as zearalenone, were obtained by gas chromatography-electron ionization mass spectrometry. Quantitation of deoxynivalenol as its trifluoroacetyl ester by selected ion monitoring was then possible down to 10 pg in the mass spectrometer (equivalent to 1 ng/g in a sample). The method was tested by extracting and analysing mouldy wheat which had previously given a positive result for deoxynivalenol when analysed by thin layer chromatography. The method was also suitable for the analysis of diacetoxyscirpenol and T-2 toxin; however, quantitation of zearalenone was unsuccessful because of the instability of the derivative.
Wreford
B J
BJ
CSIRO Division of Food Research, North Ryde, NSW, Australia.
Shaw
K J
KJ
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D012717:Sesquiterpenes / Q000032:analysis*; D013605:T-2 Toxin / Q000032:analysis; D014255:Trichothecenes / Q000032:analysis*; D015025:Zearalenone / Q000032:analysis
D012717:Sesquiterpenes; D014255:Trichothecenes; D015025:Zearalenone; D013605:T-2 Toxin; C007262:deoxynivalenol; C001809:diacetoxyscirpenol