Effects of electrical stimulation upon post-hatching development of fibre types in normally innervated fast and slow latissimus dorsii muscles of the chicken.
In chicken, the main characteristic properties of muscle fibre types in slow anterior (ALD) and fast posterior (PLD) latissimus dorsii are acquired during post-hatching development. At day 4 it becomes possible to distinguish between alpha' and beta' fibre types in ALD muscle. At the same time, mATPase staining and NADH-TR activity permit recognition of alpha w and alpha R fibres within PLD muscle. During further development, muscle fibre typology progressively changes towards the adult slow and fast type. Chronic stimulation at a slow rhythm (5 Hz) of PLD prevents the change in relative proportions of alpha R and alpha W fibres within the muscle that occurs in normal post-hatching development and increases the number of beta R fibres. Moreover, oxidative activity is increased in all muscle fibre types following stimulation. In ALD muscle, chronic stimulation at a fast rhythm (40 Hz) results in a decrease in oxidative activity and inhibits the differentiation of alpha' and beta' muscle fibre types. This study demonstrates that in young chicken, the pattern of activity influences the differenciation of fibre types in slow and fast muscles.
Khaskiye
A
A
Groupe de Physiologie Cellulaire, Faculté des Sciences, Nantes, France.
Renaud
D
D
Le Douarin
G
G
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000375:Aging; D000818:Animals; D002454:Cell Differentiation; D002645:Chickens; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D004558:Electric Stimulation; D006651:Histocytochemistry; D024510:Muscle Development; D009132:Muscles / Q000166:cytology / Q000201:enzymology; D009210:Myofibrils / Q000201:enzymology; D009928:Organ Specificity
D008058:Dihydrolipoamide Dehydrogenase; D000251:Adenosine Triphosphatases