Induction of chromosome damage and sister-chromatid exchanges in human lymphocyte cultures by the antitumour antibiotic Neocarzinostatin.
Neocarzinostatin (NCS), a chemotherapeutic antibiotic, was investigated for the ability to induce chromosomal aberrations and sister-chromatid exchanges (SCEs) in human lymphocyte cultures. It was observed that the antibiotic causes a cell-cycle delay and reduces the mitotic index. Analysis of the induced chromosomal abnormalities showed that they are mainly chromosome and chromatid breaks; while the frequency of SCEs was increased, the magnitude indicates that NCS cannot be considered a potent inducer of SCEs.
Psaraki
K
K
University of Patras, Department of Biology, Patras, Greece.
Demopoulos
N A
NA
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D004249:DNA Damage; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects; D008940:Mitotic Index / Q000187:drug effects; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects; D009353:Zinostatin / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D009153:Mutagens; D009353:Zinostatin