Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 alpha subunit subsequent to infection.
The virus-associated VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of mRNAs late after infection. Deletion mutant dl331 fails to produce this RNA and, as a result, grows poorly. Three lines of evidence suggest that VAI RNA facilitates translation by preventing inactivation of the function of eukaryotic initiation factor 2 (eIF-2). First, the mutant's translational defect can be relieved by addition of eIF-2 or eIF-2B (GTP recycling factor). Second, extracts of mutant-infected cells exhibit enhanced protein P1/eIF-2 alpha subunit kinase activity. Third, dl331 can grow with nearly normal kinetics in cells that do not express the kinase.
Schneider
R J
RJ
Safer
B
B
Munemitsu
S M
SM
Samuel
C E
CE
Shenk
T
T
D000257:Adenoviridae Infections / Q000382:microbiology*; D000258:Adenovirus Infections, Human / Q000382:microbiology*; D000260:Adenoviruses, Human / Q000235:genetics* / Q000254:growth & development; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D015852:Eukaryotic Initiation Factor-2; D005260:Female; D006801:Humans; D007700:Kinetics; D009154:Mutation; D010448:Peptide Initiation Factors / Q000378:metabolism*; D010766:Phosphorylation; D011247:Pregnancy; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*
D015852:Eukaryotic Initiation Factor-2; D010448:Peptide Initiation Factors; D011506:Proteins; D012367:RNA, Viral; D011494:Protein Kinases