The dynamic nature of DNA-strand breaks present in differentiating muscle cells and quiescent lymphocytes.
Cellular differentiation in a number of eukaryotic systems is associated with changes in the number of DNA-strand breaks and involves the activity of adenosine diphosphoribosyl transferase (ADPRT). DNA-strand breaks are essential for activation of nuclear ADPRT, the activity of which is required for efficient religation of DNA-strand breaks. In this study we demonstrate the dynamic nature of DNA-strand breaks formed in the genome of differentiating avian skeletal muscle cells and quiescent human lymphocytes. Inhibition of ADPRT activity blocks DNA-strand ligation in both cell types and leads to the accumulation of a higher number of strand breaks.
Farzaneh
F
F
Shall
S
S
Johnstone
A P
AP
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002454:Cell Differentiation; D002642:Chick Embryo; D004247:DNA / Q000032:analysis*; D004260:DNA Repair; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D009132:Muscles / Q000166:cytology*; D009713:Nucleotidyltransferases / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases
D001549:Benzamides; C025160:3-aminobenzamide; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D009713:Nucleotidyltransferases