Activation regions in a yeast transposon have homology to mating type control sequences and to mammalian enhancers.
The DNA sequence of the Ty1 activating region from the CYC7-H2 mutant of Saccharomyces cerevisiae is presented. Analysis of the data revealed the presence of four simian virus 40-type enhancer core sequences. Two of the Ty1 enhancer cores are contiguous with sequences also homologous to the diploid control site at MAT alpha. We postulate that these two Ty1 regions of approximately equal to 30 base pairs are regulatory blocks, and we have analyzed deletions to ascertain whether they are necessary for effects of Ty1 on adjacent gene expression. We found that activation is lost when a restriction fragment encompassing both postulated regulatory blocks is deleted. Deletion of restriction fragments that remove only one of the two regulatory blocks has little or no effect on Ty1 activating ability in haploid yeast cells or on repression of this function in diploid yeast cells. Because the most significant internal homologies in the restriction fragments analyzed are the putative regulatory blocks, these observations suggest that enhancer-like sequences are involved in cell-type control of Ty1 effects on gene expression.
Errede
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B
Company
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Ferchak
J D
JD
Hutchison
C A
CA
Yarnell
W S
WS
D001483:Base Sequence; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D004742:Enhancer Elements, Genetic; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005809:Genes, Regulator; D005838:Genotype; D009154:Mutation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013045:Species Specificity
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes