title: "Differential displacement"--a simple method to study receptors for 1,25-dihydroxyvitamin D3 in the presence of contaminating serum vitamin D binding protein.
abstract: This report describes a simple procedure to quantitate receptors for 1,25-dihydroxyvitamin D3 in the presence of contaminating serum vitamin D binding protein. The method ("differential displacement") takes advantage of the greatly different rates of dissociation of 1,25-dihydroxyvitamin D3 from the serum vitamin D binding protein (t 1/2 less than 5 min) and from the receptor (t 1/2 greater than 120 h) at 0 degrees C. The principle of "differential displacement" can be used for other steroid receptors as well, and in combination with a variety of different binding assays, provided they are performed under conditions where the dissociation of the steroid from the receptor is slow.
authors.forename: F O
authors.initials: FO
authors.lastname: Levy
authors.forename: L
authors.initials: L
authors.lastname: Eikvar
authors.forename: A
authors.initials: A
authors.lastname: Frøysa
authors.forename: V
authors.initials: V
authors.lastname: Hansson
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002117:Calcitriol / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism*; D014316:Tritium; D014809:Vitamin D-Binding Protein / Q000097:blood*
chemical_list: D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D014809:Vitamin D-Binding Protein; D014316:Tritium; D002117:Calcitriol