title: Effect of structure of the initiator codon on translation in E. coli.
abstract: A set of plasmids carrying different initiator codons--either AUG, or GUG, or UUG, or CUG (as a control) in the hybrid gene lacIZ--was constructed by using synthetic oligonucleotides. GUG and UUG codons were demonstrated to be 2-3 times less effective than AUG in translation initiation. Furthermore, the correlation between the efficiencies of different initiator codons in translation initiation proved to vary, depending on the phase of bacterial growth. The rarely occurring usage in nature of the initiator codons GUG and UUG is supposed to be due to the particular role played by the initiator triplets in regulation of gene expression.
authors.affiliation: D.I. Ivanovsky Institute of Virology, Academy of Medical Sciences, Moscow, USSR.
authors.lastname: Khudyakov YuE
authors.forename: V S
authors.initials: VS
authors.lastname: Neplyueva
authors.forename: T I
authors.initials: TI
authors.lastname: Kalinina
authors.forename: V D
authors.initials: VD
authors.lastname: Smirnov
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D009876:Operon; D010442:Peptide Chain Initiation, Translational; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D001616:beta-Galactosidase