Adenylylation of trospectomycin by crude enzyme preparations from Escherichia coli.
Employing osmotically shocked lysate of a spectinomycin resistant strain of Escherichia coli, trospectomycin, a new alkylspectinomycin, was adenylylated in the presence of adenosine 5'-triphosphate and magnesium ion. A highly resistant strain of E. coli was obtained by transforming a laboratory strain with a newly constructed plasmid consisting of pBR322 and a determinant for spectinomycin resistance originally found on a low copy number plasmid in E. coli strain NR79. The biologically inactive adenylylated trospectomycin was found to be trospectomycin 6-(5'-adenylate).
Yagi
Y
Y
Upjohn Company, Kalamazoo, Michigan 49001.
Baczynskyj
L
L
Mizsak
S A
SA
Argoudelis
A D
AD
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000494:pharmacology; D002474:Cell-Free System; D004351:Drug Resistance; D004926:Escherichia coli / Q000201:enzymology*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D011995:Recombination, Genetic / Q000187:drug effects; D000198:Spectinomycin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology
C057890:trospectinomycin 6-(5'-adenylate); D000249:Adenosine Monophosphate; C043267:trospectomycin; D000198:Spectinomycin