Chromosomal localization and preliminary characterization of the human gene encoding insulin-like growth factor II.
A cDNA probe corresponding to mRNA encoding a closely related variant of human insulin-like growth factor II (IGF-II) was used for the chromosomal assignment of the IGF-II gene. Southern blot hybridization analysis of DNA from human-Chinese hamster somatic cell hybrids showed that the human IGF-II gene is located on chromosome 11. Using the same IGF-II variant probe, a cosmid was isolated which contains the human IGF-II gene. Restriction enzyme analysis revealed that the gene encoding IGF-II has a discontinuous structure and contains at least four exons.
de Pagter-Holthuizen
P
P
Höppener
J W
JW
Jansen
M
M
Geurts van Kessel
A H
AH
van Ommen
G J
GJ
Sussenbach
J S
JS
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D007328:Insulin / Q000235:genetics*; D010455:Peptides / Q000235:genetics*; D010957:Plasmids; D013002:Somatomedins / Q000235:genetics*
D007328:Insulin; D010455:Peptides; D013002:Somatomedins; D004247:DNA; D004262:DNA Restriction Enzymes