title: Use of transposon mutants to assess the role of exoenzyme S in chronic pulmonary disease due to Pseudomonas aeruginosa.
abstract: Among the potential virulence factors produced by Pseudomonas aeruginosa there are two distinct ADP-ribosyl transferases, exotoxin A and exoenzyme S. The role of exoenzyme S in Pseudomonas aeruginosa infection was studied using the rat chronic pulmonary infection model. Pseudomonas aeruginosa strain DG1 and an isogenic mutant of DG1 differing only in its capacity to produce exoenzyme S were employed in the study. Both Pseudomonas aeruginosa strains tested established a chronic pulmonary infection in this model and organisms recovered from lung homogenates were phenotypically unaltered with respect to exoenzyme S production in vitro. The extent of the observed pathology was markedly greater with the strain producing exoenzyme S, indicating that exoenzyme S may play a role in the progressive pathology observed in chronic lung disease due to Pseudomonas aeruginosa.
authors.forename: D E
authors.initials: DE
authors.lastname: Woods
authors.forename: P A
authors.initials: PA
authors.lastname: Sokol
mesh_terms: D036002:ADP Ribose Transferases; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001427:Bacterial Toxins; D002908:Chronic Disease; D004251:DNA Transposable Elements; D008297:Male; D009154:Mutation; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D011014:Pneumonia / Q000382:microbiology* / Q000473:pathology; D011065:Poly(ADP-ribose) Polymerases; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000472:pathogenicity*; D051381:Rats
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; C444820:exoenzyme S; D009713:Nucleotidyltransferases