title: The metabolism of leukotrienes in blood plasma studied by high-performance liquid chromatography.
abstract: The metabolism of leukotrienes (B4, C4, D4, and E4) within human plasma was studied and a simple sample preparation is presented. It was demonstrated that leukotriene E4 and leukotriene B4 were stable during incubation at 37 degrees C using the in vitro system. In contrast, leukotriene C4 was metabolized by gamma-glutamyl transpeptidase activities into leukotriene D4 which was further metabolized by dipeptidase activities of plasma into leukotriene E4. The transition state inhibitor of gamma-glutamyl transpeptidase L-serine-borate decreased the metabolism of leukotriene C4 in plasma. Dilution of plasma demonstrated that the dipeptidase was more active compared to the gamma-glutamyl transpeptidase. The metabolizing activities of plasma were functionally characterized by fractionating the plasma proteins.
authors.forename: M
authors.initials: M
authors.lastname: Köller
authors.forename: W
authors.initials: W
authors.lastname: Schönfeld
authors.forename: J
authors.initials: J
authors.lastname: Knöller
authors.forename: K D
authors.initials: KD
authors.lastname: Bremm
authors.forename: W
authors.initials: W
authors.lastname: König
authors.forename: B
authors.initials: B
authors.lastname: Spur
authors.forename: A
authors.initials: A
authors.lastname: Crea
authors.forename: W
authors.initials: W
authors.lastname: Peters
mesh_terms: D001798:Blood Proteins / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006801:Humans; D007201:Indicator Dilution Techniques; D007975:Leukotriene B4 / Q000097:blood*; D017999:Leukotriene E4; D011489:Protein Denaturation; D013189:SRS-A / Q000031:analogs & derivatives* / Q000097:blood*
chemical_list: D001798:Blood Proteins; D013189:SRS-A; D007975:Leukotriene B4; D017999:Leukotriene E4