Rat Sertoli cells acquire a beta-adrenergic response during primary culture.
Two-dimensional polyacrylamide gel electrophoresis and the radioligand (-)-[125I]iodopindolol (125I-Pin) have been used to study isoproterenol-dependent protein phosphorylation and beta-adrenergic receptor availability, respectively, in cultured Sertoli cells and freshly isolated seminiferous tubular segments of sexually immature and mature rats. Sertoli cells prepared from sexually immature rats show progressive 125I-Pin binding in primary cultures that correlates with isoproterenol-induced cell shape changes, redistribution of immunoreactive vimentin, and phosphorylation of this intermediate filament protein. The development of 125I-Pin binding to Sertoli cell lysates is blocked by cycloheximide. Seminiferous tubules do not show significant isoproterenol-dependent vimentin phosphorylation nor 125I-Pin binding. However, vimentin phosphorylation can be induced by follicle-stimulating hormone or a cyclic nucleotide analog. This study stresses the need for correlating pharmacological-induced responses observed in Sertoli cell primary cultures with those in the intact seminiferous tubule.
Kierszenbaum
A L
AL
Spruill
W A
WA
White
M G
MG
Tres
L L
LL
Perkins
J P
JP
D000818:Animals; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010766:Phosphorylation; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000032:analysis*; D012671:Seminiferous Tubules / Q000378:metabolism; D012708:Sertoli Cells / Q000032:analysis / Q000187:drug effects*
D011506:Proteins; D011943:Receptors, Adrenergic, beta; C029247:3-iodopindolol; D005640:Follicle Stimulating Hormone; D010869:Pindolol; D007545:Isoproterenol