Cyclic-AMP-dependent protein kinase type II is associated with the Golgi complex and with centrosomes.
The subcellular distribution of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) in epithelial and fibroblastic cells was determined by indirect immunofluorescence microscopy. In interphase cells both regulatory (R II) and catalytic (C) subunits were concentrated in a perinuclear area. By comparison of the R II distribution with the location of a bona fide Golgi membrane constituent, this area was identified as the Golgi complex. The cytochemical localization of R II was confirmed by subcellular fractionation. In addition, cAMP-dPK II was associated with microtubule-organizing centers, in particular with mitotic spindle poles. These distributions of cAMP-dPK II probably represent important factors in mediating the effects of cAMP on basic cellular activities ranging from secretion and proliferation to cell shape and motility.
Nigg
E A
EA
Schäfer
G
G
Hilz
H
H
Eppenberger
H M
HM
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D002458:Cell Fractionation; D002460:Cell Line; D000242:Cyclic AMP / Q000494:pharmacology; D004848:Epithelium; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000201:enzymology*; D007399:Interphase; D008938:Mitosis; D011494:Protein Kinases / Q000032:analysis* / Q000276:immunology; D008941:Spindle Apparatus / Q000201:enzymology*
D000906:Antibodies; D000242:Cyclic AMP; D011494:Protein Kinases