Cyclic AMP and the heat shock response in Chinese hamster ovary cells.
Heat shock leads to transient increases in cAMP levels in HA-1-CHO cells. Such pulses are correlated temporally with the induction of heat resistance (thermotolerance) and with heat shock protein synthesis. Although the kinetics of cAMP increase after heating suggest a role in thermotolerance induction, raising cAMP levels directly using dBcAMP did not produce full thermotolerance. The resistance induced by dBcAMP may thus be either a component of or different to heat-shock triggered resistance. Cells which had been made thermotolerant by heat shock did not produce a pulse in cAMP level on heating. The cAMP producing system thus seemed desensitized to heat in thermotolerant cells.
Calderwood
S K
SK
Stevenson
M A
MA
Hahn
G M
GM
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006358:Hot Temperature; D007700:Kinetics; D010053:Ovary
D006360:Heat-Shock Proteins; D003994:Bucladesine; D000242:Cyclic AMP