Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization.
Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0 degree projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 A. Our structural results are in agreement with the model of Henderson et al. [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.
Kim
C H
CH
Radermacher
M
M
Kessel
M
M
Frank
J
J
King
T E
TE
D019826:Cholic Acid; D002793:Cholic Acids; D003460:Crystallization; D003576:Electron Transport Complex IV; D005583:Fourier Analysis; D008081:Liposomes; D008854:Microscopy, Electron; D008958:Models, Molecular; D012995:Solubility
D002793:Cholic Acids; D008081:Liposomes; D003576:Electron Transport Complex IV; D019826:Cholic Acid