[Rpo pathway as a possible recombination mechanism of the interaction between DNA of transducing phages and the Escherichia coli genome].
The conditions affecting recombination of DNAs of transducing lambdoid rifd phages with the chromosome of Escherichia coli K-12 recA in the region of homology were studied. In support to the previously obtained data, the Int system was shown to take no part in the process. The homologous character of recombination interactions and their dependence on efficiency of transcription were demonstrated. It is therefore suggested that recombination takes the Rpo pathway. Similar peculiarities were revealed in the processes of interaction between DNAs of rifd phages as well as of lambdoid phages carrying trp genes and the host genome. A hypothesis is put forward that the Rpo pathway operates, depending on the density of DNA supercoiling.
Kholodiĭ
G Ia
GIa
Mindlin
S Z
SZ
D010582:Bacteriophage lambda / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008957:Models, Genetic; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D004279:DNA, Viral
Rpo-put' kak vozmozhnyĭ rekombinatsionnyĭ mekhanizm vo vzaimodeĭstviiakh DNK transdutsiruiushchikh fagov s genomom Escherichia coli.