[Production of immobilized SalI and PvuII restrictase preparations].
Conditions for the immobilization of specific endonucleases Sal I and Pvu II on BrCN-activated Sepharose 4B have been selected. Some physico-chemical properties of the preparations of immobilized restrictases Sal I and Pvu II have been characterized. The specific and general activity values of the preparations thus obtained have been established. The immobilized enzymes have been used for the multiple restriction of the DNA of phage lambda and the DNA of Neisseria meningitidis.
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D010582:Bacteriophage lambda / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000494:pharmacology; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004800:Enzymes, Immobilized / Q000302:isolation & purification* / Q000494:pharmacology; D006868:Hydrolysis; D009345:Neisseria meningitidis / Q000187:drug effects; D011514:Proteus vulgaris / Q000201:enzymology; D013302:Streptomyces / Q000201:enzymology
D004269:DNA, Bacterial; D004279:DNA, Viral; D004800:Enzymes, Immobilized; D004262:DNA Restriction Enzymes; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases
Poluchenie preparatov immobilizovannykh restriktaz SalI i PvuII.