title: Selective degradation of T cell antigen receptor chains retained in a pre-Golgi compartment.
abstract: We have examined the fate of newly synthesized T cell antigen receptor (TCR) subunits in a T cell hybridoma deficient in expression of the clonotypic beta chain. Synthesis and assembly of the remaining chains proceed normally but surface expression of TCR chains is undetectable in these cells. A variety of biochemical and morphological techniques has been used to show that the TCR chains in these cells fail to be transported to any of the Golgi cisternae. Instead, they are retained in a pre-Golgi compartment which is either part of or closely related to the endoplasmic reticulum. The CD3-delta chain is degraded by a non-lysosomal process that is inhibited at temperatures at or below 27 degrees C. By contrast, the remaining chains (CD3-epsilon, CD3-gamma, and zeta) are very stable over 7 h. We propose possible mechanisms that may explain the differential fate of TCR chains retained in a pre-Golgi compartment.
authors.affiliation: Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: J S
authors.initials: JS
authors.lastname: Bonifacino
authors.forename: L C
authors.initials: LC
authors.lastname: Yuan
authors.forename: R D
authors.initials: RD
authors.lastname: Klausner
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D001692:Biological Transport; D017252:CD3 Complex; D002451:Cell Compartmentation; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006825:Hybridomas; D008247:Lysosomes / Q000187:drug effects; D046911:Macromolecular Substances; D051379:Mice; D008854:Microscopy, Electron; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism* / Q000648:ultrastructure; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000378:metabolism*; D013696:Temperature
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell