Immunocytochemical localization of the Ca2+-ATPase polypeptide in human platelets.
Specific polyclonal antibodies raised against purified human platelet Ca2+-ATPase were used with protein A-gold immunocytochemistry to localize this protein in human platelets. Immunolabeling specifically detected Ca2+-ATPase over the surface connected membrane system (SCS) in sections of paraformaldehyde-fixed, Lowicryl-embedded platelets. The maximum density of label, determined by quantitative morphometric techniques, was observed over electron-dense regions within the SCS which may represent specialized structures for uptake and release of Ca2+. Less intense immunolabeling was observed over cytosol and may represent localization over the dense tubular system (DTS) which was not readily visualized under the processing procedures employed.
Herbener
G H
GH
Department of Anatomical Sciences, University of Louisville School of Medicine, KY 40292.
Dean
W L
WL
D001792:Blood Platelets / Q000201:enzymology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002451:Cell Compartmentation; D006801:Humans; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D007425:Intracellular Membranes / Q000201:enzymology; D008854:Microscopy, Electron
D000252:Calcium-Transporting ATPases