title: Inhibition of growth of Chlamydia trachomatis by nonoxynol-9 in vitro.
abstract: We evaluated the ability of the widely used spermicide nonoxynol-9, Conceptrol gel containing nonoxynol-9, and Conceptrol vehicle (without nonoxynol-9) to inhibit the formation of inclusions of Chlamydia trachomatis in cycloheximide-treated McCoy cells. Conceptrol vehicle produced a non-dose-related 40 to 59% reduction of the number of inclusions formed. In contrast, the addition of nonoxynol-9 and Conceptrol gel containing nonoxynol-9 at a concentration of 100 micrograms/ml reduced the number of inclusions by 84 to 95%. Prolongation of contact between nonoxynol-9 and the chlamydial organisms from 90 to 180 min did not result in additional inhibition. Using a higher concentration of chlamydial organisms and diluting the drug-chlamydia mixture before inoculation of the monolayers, we were able to test higher concentrations of nonoxynol-9. At concentrations of 100 to 5,000 micrograms of nonoxynol-9 per ml, a dose-related 19 to 84% reduction of the number of inclusions was noted.
authors.forename: S
authors.initials: S
authors.lastname: Benes
authors.forename: W M
authors.initials: WM
authors.lastname: McCormack
mesh_terms: D002692:Chlamydia trachomatis / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D017137:Nonoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013089:Spermatocidal Agents / Q000494:pharmacology*
chemical_list: D013089:Spermatocidal Agents; D017137:Nonoxynol; D011092:Polyethylene Glycols