title: A vehicle for the introduction of transposons into plant-associated pseudomonads.
abstract: A recombinant plasmid with wide-host-range transfer functions, narrow-host-range replication functions, and carrying a kanamycin-resistant transposon transferred kanamycin resistance to a number of plant-associated pseudomonads. Southern hybridization studies suggest that only a small portion of the plasmid, coinciding with the location of the transposon, is present in the kanamycin-resistant Pseudomonas derivatives. The plasmid sequences appear to be inserted at a number of different sites in the recipient genome. This plasmid can thus be used as a vehicle for the introduction of transposons into some plant-associated pseudomonads and should be useful in both genetic and ecological studies of these bacteria.
authors.forename: S T
authors.initials: ST
authors.lastname: Lam
authors.forename: B S
authors.initials: BS
authors.lastname: Lam
authors.forename: G
authors.initials: G
authors.lastname: Strobel
mesh_terms: D004251:DNA Transposable Elements; D005822:Genetic Vectors; D007612:Kanamycin / Q000494:pharmacology; D010944:Plants / Q000382:microbiology; D011549:Pseudomonas / Q000235:genetics*; D011815:R Factors
chemical_list: D004251:DNA Transposable Elements; D007612:Kanamycin