title: Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.
abstract: A series of human carcinoma cell lines was examined for human papillomavirus (HPV) DNA sequences with the use of HPV-6, HPV-11, HPV-16, and HPV-18 DNA probes. Six of eight cell lines derived from human cervical carcinomas were shown to contain integrated HPV DNA sequences. In five of these six lines, HPV-specific polyadenylated RNA species could also be identified. The expression of HPV sequences was detected in three lines with a HPV-18 DNA probe and in two lines with a HPV-16 DNA probe. Of the two lines which contained HPV-16 specific RNA, one contained HPV DNA sequences which hybridized only to an HPV-16 probe, and the other contained HPV DNA sequences which hybridized to both HPV-16 and HPV-18 DNA probes. Six cell lines established from human squamous-cell carcinomas of the bladder, pharynx, lung, esophagus, and vulva were negative for HPV-6, HPV-11, HPV-16, and HPV-18 DNA sequences under stringent hybridization conditions.
authors.forename: C
authors.initials: C
authors.lastname: Yee
authors.forename: I
authors.initials: I
authors.lastname: Krishnan-Hewlett
authors.forename: C C
authors.initials: CC
authors.lastname: Baker
authors.forename: R
authors.initials: R
authors.lastname: Schlegel
authors.forename: P M
authors.initials: PM
authors.lastname: Howley
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral