title: MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited by EGF.
abstract: Epidermal growth factor (EGF) has been noted to stimulate proliferation of a variety of normal and malignant cells including those of human breast epithelium. We report here that MDA-468, a human breast cancer cell line with a very high number of EGF receptors, is growth-inhibited at EGF concentrations that stimulate most other cells. The basis for the elevated receptor level is EGF receptor gene amplification and over-expression. An MDA-468 clone selected for resistance to EGF-induced growth inhibition shows a number of receptors within the normal range. The results are discussed in relation to a threshold model for EGF-induced growth inhibition.
authors.forename: J
authors.initials: J
authors.lastname: Filmus
authors.forename: M N
authors.initials: MN
authors.lastname: Pollak
authors.forename: R
authors.initials: R
authors.lastname: Cailleau
authors.forename: R N
authors.initials: RN
authors.lastname: Buick
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015247:Deoxyribonuclease HindIII; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors; D005784:Gene Amplification; D006801:Humans; D011956:Receptors, Cell Surface / Q000235:genetics*
chemical_list: D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D004247:DNA; D066246:ErbB Receptors; D004262:DNA Restriction Enzymes; D015247:Deoxyribonuclease HindIII