title: Rhythmic cyclic AMP changes in Chlamydomonas cells synchronized by temperature and light cycles in chemostat culture.
abstract: Division in chemostat cultures of Chlamydomonas reinhardi was synchronized by daily cycles of both temperature and light. In response to 8 h cool (20 degrees C) - dim light/16 h warm (28 degrees C) - bright light cycles and continuous dilution that removed half the cells daily, the cells replicated nuclear DNA late in the warm-bright phase; released an average of two daughter zoospores early in the warm-bright phase of the next cycle. In these synchronized green algal cultures all ATP and most cAMP were intracellular; most cGMP (approximately 91%) was extracellular. Early in the warm-bright phase ATP doubled after which cAMP level fell 25%. During DNA replication cAMP level doubled, rising to a modest peak reached as replication ended.
authors.forename: M A
authors.initials: MA
authors.lastname: Sharaf
authors.forename: D W
authors.initials: DW
authors.lastname: Rooney
mesh_terms: D002453:Cell Cycle; D002478:Cells, Cultured; D002696:Chlamydomonas / Q000166:cytology / Q000378:metabolism*; D002940:Circadian Rhythm; D000242:Cyclic AMP / Q000378:metabolism*; D004261:DNA Replication; D008027:Light; D013696:Temperature
chemical_list: D000242:Cyclic AMP