Visualization of reversible macromolecular reactions in an analytical ultracentrifuge.
It has been shown that the saw-like disturbances of sedimentation observed in an analytical ultracentrifuge are not caused by convective disturbances of the solution but result from a special type of intermolecular reaction of reversible association/dissociation. A qualitative theory of saw-like anomalies has been suggested and the sedimentation and kinetic conditions of their origin have been indicated. Such reactions are a frequent occurrence in the serum of patients affected with rheumatic diseases and acute myocardial infarction. Experimental data indicate the involvement of immunoglobulins (viz., low-affinity antibodies) which form reversible immune complexes. Saw-like sedimentation patterns, especially those of the schlieren type, are a direct testimony to reversible association/dissociation reactions in macromolecular solutions, whereas other experimental methods provide only oblique evidence.
Chernyak
V Y
VY
A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Kogan-Yasny
V V
VV
D000915:Antibody Affinity; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D002499:Centrifugation, Density Gradient; D006801:Humans; D007105:Immune Complex Diseases / Q000097:blood; D007136:Immunoglobulins; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D008433:Mathematics; D009203:Myocardial Infarction / Q000097:blood / Q000276:immunology; D014461:Ultracentrifugation
D007136:Immunoglobulins