title: Cloned DNA sequences that determine mRNA stability of bacteriophage phi X174 in vivo are functional.
abstract: The stability of two species of phi X174 polycistronic mRNA in vivo can be altered by mutating sequences existing immediately upstream of a termination site. The wild type phage contains an mRNA stabilizing sequence ((+) sequence), while the same sequence mutated by insertion ((-) sequence) reduces the stability of the mRNAs. These two sequences were cloned at the 3' ends of gene D or gene B of phi X174 in a pBR322 derivative plasmid. The cloned sequences were functional. The (+) sequence stabilized gene B or gene D mRNA; half-lives of these mRNAs were 7 to 8 min. When the (+) sequence is eliminated ((o) sequence) or replaced with the (-) sequence, the half-lives of the mRNA were reduced to about 1 to 2 min. The stabilization of mRNAs caused an increased production of these proteins.
authors.forename: M N
authors.initials: MN
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004355:Drug Stability; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes