title: Correlation of laser-Doppler-velocity measurements and endothelial cell shape in a stenosed dog aorta.
abstract: Laser-Doppler-velocity measurements were carried out in an elastic 1:1 true-to-scale silicone rubber model of a dog aorta with stenosis. The model was constructed from a cast of a severely stenosed dog aorta (71% of its area). The stenosis in the dog aorta was prepared by wrapping a cotton band around the aorta. This band was tightened until the presence of a thrill or a bruit was felt distal to the band. Twelve weeks later the animal was sacrificed and a cast was prepared from the aorta. From this vascular cast, the cross-sectional area was calculated. Endothelial cell geometry and orientation was studied using computerized analysis to determine the cell area and shape index. An elastic silicone rubber model was prepared from the cast to measure the velocity profiles and to estimate the local wall shear stress. Velocity measurements were done at steady and pulsatile flow using a Newtonian aqueous-glycerol solution and a non-Newtonian blood-like fluid. From those velocity measurements the velocity gradients near the wall were determined and the shear stress calculated. The flow distal to the stenosis separates from the wall at physiological conditions. The endothelial cells are smaller and more elongated in the throat; distal to the stenosis they are larger and rounder. The shape index distribution along the stenosed aorta is correlated with the level of wall shear stress. It is shown that even low changes in the wall shear stress have an influence on the orientation of the endothelial cells.
authors.affiliation: Hal B. Wallis Research Facility, Eisenhower Medical Ctr., Rancho Mirage, CA 92270.
authors.forename: D W
authors.initials: DW
authors.lastname: Liepsch
authors.forename: M
authors.initials: M
authors.lastname: Levesque
authors.forename: R M
authors.initials: RM
authors.lastname: Nerem
authors.forename: S T
authors.initials: ST
authors.lastname: Moravec
mesh_terms: D000818:Animals; D001011:Aorta / Q000503:physiopathology* / Q000648:ultrastructure; D001018:Aortic Diseases / Q000503:physiopathology*; D001783:Blood Flow Velocity; D004285:Dogs; D004730:Endothelium, Vascular / Q000648:ultrastructure*; D007834:Lasers; D008026:Ligation; D008953:Models, Anatomic; D008955:Models, Cardiovascular; D012212:Rheology; D013314:Stress, Mechanical; D014465:Ultrasonics