Activation of major histocompatibility complex class I mRNA containing an Alu-like repeat in polyoma virus-transformed rat cells.
Class I genes of the major histocompatibility complex (MHC) appear to be activated in mouse cells transformed by the DNA tumour virus simian virus 40 (SV40). Conversely, suppression of MHC class I genes has been reported in adenovirus-12-transformed baby kidney rat cells. We have now investigated the expression of genes encoded by the rat MHC locus in rat fibroblast cells transformed by polyoma virus (Py). Using a mouse genomic H-2 clone as a probe in Northern transfer hybridization analysis, we have observed a high level of expression of rat MHC class I messenger RNA in all the transformed rat cell lines analysed. The class I 1.6-kilobase (kb) mRNA activated in Py-transformed rat cells appears to contain an Alu-like type II repeat element, as the same 1.6-kb mRNA is detected using either the H-2 class I sequence or a repetitive Alu-like type II element as a probe. High levels of heterogeneous poly(A)+ transcripts of 0.5-0.8 kb are also observed in Py-transformed rat cells using probes containing an Alu-like type II repetitive element.
Majello
B
B
La Mantia
G
G
Simeone
A
A
Boncinelli
E
E
Lania
L
L
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006649:Histocompatibility Antigens / Q000235:genetics*; D008285:Major Histocompatibility Complex; D011120:Polyomavirus; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid
D000957:Antigens, Viral, Tumor; D006649:Histocompatibility Antigens; D012333:RNA, Messenger