title: The basolateral membrane of rat enterocyte: its purification from brush border contamination.
abstract: Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaCl2 to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low L-glucose permeability across basolateral membrane vesicles together with ATP-dependent sodium uptake was observed.
authors.forename: M N
authors.initials: MN
authors.lastname: Orsenigo
authors.forename: M
authors.initials: M
authors.lastname: Tosco
authors.forename: G
authors.initials: G
authors.lastname: Esposito
authors.forename: A
authors.initials: A
authors.lastname: Faelli
mesh_terms: D000818:Animals; D002118:Calcium; D002462:Cell Membrane / Q000032:analysis* / Q000201:enzymology; D002498:Centrifugation; D005947:Glucose / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism; D012979:Sodium Radioisotopes; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D012979:Sodium Radioisotopes; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D002118:Calcium