Detection of lecithin: cholesterol acyltransferase (LCAT) in a human hepatoma cell line.
A human hepatoma cell line (HepG-2) was probed for the presence of lecithin: cholesterol acyltransferase (LCAT) using an antiserum to human plasma LCAT. Double immunodiffusion analysis using antiserum to human plasma LCAT revealed a single precipitin line in the sonicated cell homogenate. This precipitin line showed a reaction of identity with highly purified plasma LCAT. The presence of LCAT within the hepatoma cells was also confirmed by an immunofluorescence test. In contrast, the cell culture supernate showed a weak and inconsistent precipitin line. These data suggest that HepG-2 cells synthesize LCAT but secretion of the enzyme by these cells into the culture medium may be partially or totally impaired.
Koren
E
E
McConathy
W J
WJ
Lacko
A G
AG
Knowles
B
B
D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008113:Liver Neoplasms / Q000201:enzymology*; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000032:analysis*
D007862:Phosphatidylcholine-Sterol O-Acyltransferase