title: Influence of Neurospora endonuclease on trenimon-induced structural chromosomal aberrations and sister-chromatid exchanges in human peripheral lymphocytes and Chinese hamster ovary cells.
abstract: Human peripheral lymphocytes and Chinese hamster ovary cells were treated in the G1 phase of the cell cycle with the trifunctional alkylating agent trenimon (TRN) and post-treated with a single-strand specific endonuclease from Neurospora crassa (NE). TRN induces chromosomal aberrations of the chromatid type (CA) and sister-chromatid exchanges (SCE). NE post-treatment leads to an elevation of the frequencies of CA but not of SCEs. This indicates that TRN induced CA are the result of DNA double-strand breaks and that the SCEs originate from other types of lesions, most probably base damage.
authors.forename: C
authors.initials: C
authors.lastname: Nowak
authors.forename: G
authors.initials: G
authors.lastname: Obe
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D004277:DNA, Single-Stranded / Q000235:genetics*; D004720:Endonucleases; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D015719:Single-Strand Specific DNA and RNA Endonucleases; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D014228:Triaziquone / Q000633:toxicity*
chemical_list: D000477:Alkylating Agents; D004277:DNA, Single-Stranded; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014228:Triaziquone