title: A site-specific endonuclease from Pseudomonas aeruginosa.
abstract: PaeI, a new restriction endonuclease from Pseudomonas aeruginosa clinical strain was isolated and characterized. It recognizes and cleaves the sequence 5'-GCATG reduced C-3' generating DNA fragments with 3'-tetranucleotide sticky ends. DNAs of pBR322, SV40 and bacteriophage lambda have one, two and six PaeI recognition sites, respectively. Seventy-two strains of Pseudomonas, Clostridium, Escherichia coli, Shigella, Proteus and Saccharomyces were screened for the presence of site-specific endonucleases. Here we describe the PaeI restriction enzyme found in Pseudomonas aeruginosa; other data will be published elsewhere. Earlier Hinkle and Miller isolated from P. aeruginosa a PaeR7 restriction endonuclease recognizing and cleaving a sequence 5'-C reduced TCGAG-3' (1). Sequence analysis of DNAs cleaved by PaeI shows that the enzyme is the isoschizomer of SphI (2).
authors.forename: N N
authors.initials: NN
authors.lastname: Sokolov
authors.forename: A B
authors.initials: AB
authors.lastname: Fitsner
authors.forename: N V
authors.initials: NV
authors.lastname: Anikeitcheva
authors.lastname: Choroshoutina YuB
authors.forename: O T
authors.initials: OT
authors.lastname: Samko
authors.forename: V O
authors.initials: VO
authors.lastname: Kolosha
authors.forename: I
authors.initials: I
authors.lastname: Fodor
authors.forename: I I
authors.initials: II
authors.lastname: Votrin
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C046184:endodeoxyribonuclease PaeI; D015252:Deoxyribonucleases, Type II Site-Specific