Detection of IgA heavy chain constant region genes in IgA deficient donors: evidence against gene deletions.
Sixty-six donors with selective IgA deficiency and one patient with selective IgA2 deficiency were investigated for immunoglobulin gene defects using restriction enzyme digestions and Southern blot analysis. All patients carried alpha 1 and alpha 2 genes in their genome, suggesting that large deletions are uncommon causes for IgA deficiency. Digestion with Bam HI, Pst I and Pvu II, did not reveal any polymorphism in the studied samples.
Hammarström
L
L
Carlsson
B
B
Smith
C I
CI
Wallin
J
J
Wieslander
L
L
D002872:Chromosome Deletion; D004247:DNA; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004406:Dysgammaglobulinemia / Q000235:genetics*; D005802:Genes, MHC Class II; D006801:Humans; D017098:IgA Deficiency; D007070:Immunoglobulin A / Q000235:genetics; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics*; D008214:Lymphocytes / Q000276:immunology; D008970:Molecular Weight
D007070:Immunoglobulin A; D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins; D004247:DNA; D004262:DNA Restriction Enzymes