Renal V2 vasopressin receptor proteins: identification and enrichment.
The synthesis of the tritium labelled photoreactive analogue of 1-deamino-vasopressin [1-(3-mercaptopropionic acid, 8-(N6-4-azido-phenylamidino)-lysine] vasopressin is described. This analogue retains a high affinity for hepatic V1 and renal V2 vasopressin receptors (apparent dissociation constant KD approximately 1-2 nM). A membrane protein from bovine kidney and pig kidney with an apparent relative molecular mass (Mr) of 30,000 was preferentially labelled and with lower yield a protein band with a Mr-value of 50,000 to 60,000. The photolabelled 30,000-Mr protein from bovine kidney was enriched by size-exclusion chromatography and by reversed-phase-high-performance liquid chromatography.
Fahrenholz
F
F
Max-Planck-Institut für Biophysik, Frankfurt am Main.
Kojro
E
E
Plage
G
G
Müller
M
M
D000345:Affinity Labels / Q000138:chemical synthesis / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007668:Kidney / Q000032:analysis*; D008236:Lypressin / Q000031:analogs & derivatives / Q000138:chemical synthesis; D011945:Receptors, Angiotensin / Q000032:analysis* / Q000378:metabolism; D017483:Receptors, Vasopressin; D013552:Swine
D000345:Affinity Labels; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; C053818:vasopressin, 1-(3-mercapto)propionic acid-8-(N(6)-4-azidophenylamidino)lysine-; D008236:Lypressin