The production of tumor necrosis factor-alpha by rat basophilic leukemia cells with triggering IgE receptor.
We evaluated cytotoxic factor released from rat basophilic leukemia cells (RBL) sensitized with anti-ovalbumin (OVA) mouse serum after incubation with OVA. The cytotoxic activity of this factor was completely blocked by anti-mouse tumor necrosis factor-alpha (mTNF-alpha) specific antibody. Therefore, we concluded that by triggering the IgE receptor, RBL could produce and release TNF-alpha.
Ohno
I
I
The First Department of Internal Meicine, Tohoku University School of Medicine, Sendai.
Tanno
Y
Y
Yamauchi
K
K
Ida
S
S
Takishima
T
T
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology*; D007073:Immunoglobulin E / Q000276:immunology*; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism*; D051381:Rats; D011961:Receptors, Fc / Q000502:physiology*; D017455:Receptors, IgE; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000944:Antigens, Differentiation, B-Lymphocyte; D011961:Receptors, Fc; D017455:Receptors, IgE; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E