The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent.
Four rat mAb directed against mouse IL-5 have been characterized by their ability to remove and neutralize mouse IL-5 activity in various bioassays. All four mAb absorbed IL-5 activity from solution. Although all were able to neutralize mouse IL-5 bioactivity, two were significantly more effective. These two were also able to neutralize the activity of mouse IL-5 delivered to B cells during a cognate-linked interaction with a Th cell clone. A two-site sandwich ELISA specific for mouse IL-5 was developed by using pairs of mAb. The mouse IL-5 ELISA is capable of detecting natural or mouse rIL-5 in supernatants, crude bacterial lysates, and high concentrations of mouse serum, and has a detection limit of less than 20 pg. Two of these antibodies cross-reacted with and neutralized human rIL-5, and one of these was used for development of an ELISA for human IL-5.
Schumacher
J H
JH
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304-1140.
O'Garra
A
A
Shrader
B
B
van Kimmenade
A
A
Bond
M W
MW
Mosmann
T R
TR
Coffman
R L
RL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000502:physiology*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D001774:Blood Chemical Analysis; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000032:analysis; D007164:Immunosorbents / Q000302:isolation & purification; D015848:Interleukin-5; D007378:Interleukins / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D051381:Rats; D011917:Rats, Inbred Lew; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007164:Immunosorbents; D015848:Interleukin-5; D007378:Interleukins