[Purine metabolism and blastogenesis in lymphocytes of alcoholic subjects].
Immune deficiency is characteristic of alcoholic subjects. These subjects usually show altered lymphocyte function. We determined the activities of adenosine deaminase (ADA) and ecto-5'-nucleotidase (ecto-5'N) in lymphocytes from 54 subjects: 15 healthy controls, 28 non-cirrhotic alcoholics, 8 alcoholic cirrhotics and 3 non-alcoholic cirrhotics. Whereas ADA activity was the same for all 54 subjects, ecto-5'N activity was in general lower in alcoholic subjects after cessation of alcohol intake. Following alcohol intoxication, however, ecto-5'N activity increased. The decrease of ecto-5'N activity in alcoholic subjects might be explained by shedding of the ecto-enzyme and alteration of lymphocyte subpopulations. We observed decreased mitogenic-induced lymphoblastic transformation in 3 patients with cirrhosis. All other subjects (including healthy controls) had normal mitogenic-induced blastogenesis. Interestingly, following alcohol intake, non-stimulated lymphoblastic transformation increased, leading to an apparently decreased stimulation index.
Levallois
C
C
Dornand
J
J
Ngou
J
J
Mani
J C
JC
Balmes
J L
JL
D015720:5'-Nucleotidase; D000243:Adenosine Deaminase / Q000097:blood; D000437:Alcoholism / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000276:immunology; D008104:Liver Cirrhosis, Alcoholic / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D009708:Nucleotidases / Q000097:blood; D011687:Purines / Q000097:blood*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology
D011687:Purines; D009708:Nucleotidases; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase
Métabolisme des purines et blastogénèse des lymphocytes chez les sujets éthyliques.