Antibodies to human T lymphotropic virus type III demonstrated by a dot immunobinding assay.
A dot immunobinding (DIB) technique was applied to the demonstration of antibodies to human T-lymphotropic virus type III (HTLV-III). By this technique IgG antibodies to HTLV-III were demonstrated in six of six Swedish patients with acquired immune deficiency syndrome and in 37 of 39 (95%) Swedish homosexual men with persistent generalized lymphadenopathy but in none of 50 Swedish healthy blood donors. Findings obtained by this method showed complete concordance with those obtained by an enzyme-linked immunosorbent assay. All sera positive by DIB were also positive by Western blotting. The DIB assay is simple and well suited for screening of sera for HTLV-III antibodies.
Biberfeld
G
G
Bredberg-Råden
U
U
Böttiger
B
B
Biberfeld
P
P
Morfeldt-Månsson
L
L
Suni
J
J
Vaheri
A
A
Saxinger
C
C
Gallo
R
R
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000382:microbiology; D000914:Antibodies, Viral / Q000032:analysis*; D001782:Blood Donors; D017977:Deltaretrovirus / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D008206:Lymphatic Diseases / Q000276:immunology* / Q000382:microbiology; D008297:Male
D000914:Antibodies, Viral