Increased release of hydrogen peroxide and superoxide anion from asbestos-primed macrophages. Effect of hydrogen peroxide on the functional activity of alpha 1-protease inhibitor.
The ability of asbestos-elicited murine peritoneal macrophages to release superoxide anion and hydrogen peroxide, following in vitro triggering, has been investigated. The asbestos-elicited macrophages produced increased levels of superoxide and hydrogen peroxide compared to control macrophages and similar levels to those produced by Corynebacterium parvum elicited macrophages. The supernatants from asbestos-elicited macrophages which had been triggered in vitro were capable of impairing the ability of alpha 1-protease inhibitor to inhibit elastase function. The catalase sensitivity of this effect showed it to be due to hydrogen peroxide.
Donaldson
K
K
Slight
J
J
Hannant
D
D
Bolton
R E
RE
D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D002374:Catalase; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D013481:Superoxides / Q000378:metabolism*; D000515:alpha 1-Antitrypsin / Q000378:metabolism
D000515:alpha 1-Antitrypsin; D013481:Superoxides; D001194:Asbestos; D006861:Hydrogen Peroxide; D002374:Catalase; D010196:Pancreatic Elastase