Monoclonal antibodies in enzyme research: present and potential applications.
Hybridoma antibodies are powerful tools. Their impact is already apparent in many areas of basic and applied research. In contrast, their impact is just beginning to be felt in enzymology. The existing literature on monoclonal antibodies to enzymes and isozymes, reviewed in this article, is as yet largely descriptive. However, the potential applications discussed herein promise to revolutionize existing strategies of unraveling the basic biochemistry, immunochemistry, and developmental, somatic cell, and molecular genetics of enzymes and isozymes. At a clinical level, monoclonal antibodies to enzymes promise to radically improve the current modalities of diagnosis and therapy in clinical enzymology and oncology. It is becoming increasingly apparent that the future applications of hybridoma antibodies to enzymes and isozymes appear to be limited only by our imagination.
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D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis; D000110:Acetylcholinesterase / Q000032:analysis; D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D002795:Choline O-Acetyltransferase / Q000032:analysis; D002846:Chromatography, Affinity; D004259:DNA-Directed DNA Polymerase / Q000032:analysis; D004798:Enzymes / Q000032:analysis* / Q000276:immunology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007527:Isoenzymes / Q000032:analysis; D010651:Phenylalanine Hydroxylase / Q000032:analysis; D010732:Phosphofructokinase-1 / Q000032:analysis; D010960:Plasminogen Activators / Q000032:analysis
D000911:Antibodies, Monoclonal; D004798:Enzymes; D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase; D010651:Phenylalanine Hydroxylase; D002795:Choline O-Acetyltransferase; D010732:Phosphofructokinase-1; D004259:DNA-Directed DNA Polymerase; D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D010960:Plasminogen Activators