The locus encoding alpha A-crystallin is closely linked to H-2K on mouse chromosome 17.
We have used a complementary DNA (cDNA) for mouse alpha A-crystallin to probe genomic DNA for restriction fragment length polymorphisms which could be used to map the alpha A-crystallin gene locus (Acry-1) in the mouse genome. Ten of 12 restriction endonucleases produced fragment polymorphism among various inbred strains of mice. A comprehensive strain survey conducted with six endonucleases resulted in the discovery of six allelic forms of Acry-1. Linkage analysis was conducted on DNA from three sets of recombinant inbred strains of mice and demonstrated close linkage of Acry-1 with the major histocompatibility complex (H-2) on chromosome 17. Analysis of congenic and recombinant congenic strains of mice confirmed the linkage of Acry-1 and H-2 and located the alpha A gene to the region between glyoxylase (Glo-1) and H-2K.
Skow
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Donner
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D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D003459:Crystallins / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D005796:Genes; D008040:Genetic Linkage; D006183:H-2 Antigens / Q000235:genetics*; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains; D011110:Polymorphism, Genetic; D014176:Protein Biosynthesis; D013045:Species Specificity
D003459:Crystallins; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D004247:DNA; D004262:DNA Restriction Enzymes