Expression of IgG Fc receptors in myeloid leukemic cell lines. Effect of colony-stimulating factors and cytokines.
Three classes of FcR have been defined on human myeloid cells by their reactivity with mAb; FcRI (mAb 32); FcRII (mAb IV3); and FcRIII (mAb 3G8). We have quantitated the expression of each FcR on human myeloid leukemia cells and cell lines (KG-1, HL-60, U937, and K562). Detailed analysis of FcR surface expression is provided for the U937 cell line after exposure to CSF and cytokines. Increased expression of FcRI and FcRII occurred at 72 h in cells exposed to GCT or Mo cell line-conditioned medium as well as to medium from PHA-treated mononuclear cells. The augmentation of FcRII required protein synthesis and was diminished by a neutralizing antibody to granulocyte-macrophage CSF. We also show that fractions containing natural granulocyte CSF or granulocyte-macrophage CSF as well as r-granulocyte and r-granulocyte-macrophage CSF are capable of inducing FcRII on these cells, whereas other cytokines such as IL-1 and IL-2, TNF-alpha, INF-gamma and macrophages CSF failed to do so.
Liesveld
J L
JL
Department of Medicine, University of Rochester School of Medicine and Dentistry, NY 14642.
Abboud
C N
CN
Looney
R J
RJ
Ryan
D H
DH
Brennan
J K
JK
D000911:Antibodies, Monoclonal / Q000502:physiology; D001666:Binding Sites, Antibody; D001688:Biological Products / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000276:immunology / Q000502:physiology*; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D016207:Cytokines; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology; D007958:Leukocyte Count; D009000:Monocytes / Q000378:metabolism / Q000473:pathology; D011961:Receptors, Fc / Q000032:analysis* / Q000187:drug effects / Q000276:immunology; D017452:Receptors, IgG; D011994:Recombinant Proteins / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D001688:Biological Products; D003115:Colony-Stimulating Factors; D003470:Culture Media; D016207:Cytokines; D011961:Receptors, Fc; D017452:Receptors, IgG; D011994:Recombinant Proteins; D003513:Cycloheximide