title: Leishmania mexicana: purine-metabolizing enzymes of amastigotes and promastigotes.
abstract: Cultured promastigote and isolated amastigote forms of Leishmania mexicana mexicana have been surveyed for the presence of enzymes involved in purine metabolism. Quantitative but not qualitative differences between the enzymes of two forms were discovered. There were found to be significant differences between the enzyme content of L. m. mexicana and that reported for L. donovani. Extracts of both parasite forms of L. m. mexicana were found to have higher levels of adenine deaminase (EC 3.5.4.2) and guanine deaminase (EC 3.5.4.3) than adenosine deaminase (EC 3.5.4.4). There appeared to be two distinct nucleosidases (EC 3.2.2.1), one active on nucleosides, the other on deoxynucleosides. Phosphorylase (EC 2.4.2.1) could be detected only in the catabolic direction. Nucleotidases were present, but were more active on 3' (EC 3.1.3.6)- than 5' (EC 3.1.3.5)-nucleotides. Phosphoribosyltransferase (EC 2.4.2.7,.8 and .22) and nucleoside kinase (EC 2.7.1.20) activities were detected in both forms. Nucleotide-interconverting enzymes were found to be present, with IMP dehydrogenase (EC 1.2.1.14) being the most active. Cell fractionation experiments revealed that, in the promastigote, enzyme separation within the parasite may play an important part in regulating cellular purine metabolism.
authors.forename: H F
authors.initials: HF
authors.lastname: Hassan
authors.forename: G H
authors.initials: GH
authors.lastname: Coombs
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism; D000619:Aminohydrolases / Q000378:metabolism; D000818:Animals; D006148:Guanine Deaminase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007891:Leishmania / Q000201:enzymology* / Q000254:growth & development; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009708:Nucleotidases / Q000378:metabolism; D010430:Pentosyltransferases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011687:Purines; D010430:Pentosyltransferases; C022484:purine phosphoribosyltransferase; D011683:Purine-Nucleoside Phosphorylase; D010770:Phosphotransferases; C021892:nucleoside phosphotransferase; D009708:Nucleotidases; D009699:N-Glycosyl Hydrolases; C030681:purine nucleosidase; D000619:Aminohydrolases; C018774:adenine deaminase; D006148:Guanine Deaminase; D000243:Adenosine Deaminase