Human recombinant interleukin 4 induces Fc epsilon R2/CD23 on normal human monocytes.
rIL-4 (B cell stimulatory factor 1) induces the expression of Fc epsilon R2/CD23 on normal human monocytes (Mo). Fc epsilon R2/CD23 induction was detectable both by flow cytometry using anti-CD23 mAbs as well as soluble IgE, and by the immunoprecipitation with CD23-specific mAb or IgE of a 45-kD band from 125I-lactoperoxidase-labeled Mo. Fc epsilon R2/CD23 was fully expressed after a 24-h incubation with rIL-4, and was still detectable after 72 h from the addition of IL-4. This effect was specific, because none of the other rILs tested (IL-1, IL-2, IL-3, IL-5, B cell stimulatory factor 2, granulocyte-macrophage colony stimulating factor, and IFN-gamma) could induce FC epsilon R2/CD23, either alone or in various combinations. No synergism was observed between IL-4 and other ILs. IFN-gamma was not able to inhibit the IL-4-induced expression of Fc epsilon R2/CD23 on Mo, neither when added to the culture together with IL-4, nor when added 36 h earlier.
Vercelli
D
D
The Division of Allergy and Immunology, Children's Hospital, Boston, Massachusetts.
Jabara
H H
HH
Lee
B W
BW
Woodland
N
N
Geha
R S
RS
Leung
D Y
DY
D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008222:Lymphokines / Q000494:pharmacology; D011961:Receptors, Fc / Q000096:biosynthesis*; D017455:Receptors, IgE; D011994:Recombinant Proteins / Q000494:pharmacology; D013268:Stimulation, Chemical
D007378:Interleukins; D008222:Lymphokines; D011961:Receptors, Fc; D017455:Receptors, IgE; D011994:Recombinant Proteins; D015847:Interleukin-4; D007073:Immunoglobulin E