Restriction fragment length polymorphism of C4 genes in mice with t chromosomes.
Genomic DNA was isolated from 29 t strains and 4 congenic lines of mice, digested with restriction endonucleases, and hybridized with a probe representing the complement component 4 (C4) gene. All but one of the enzymes revealed restriction fragment length polymorphism in this sample of C4-related genes. Double digestion analysis suggested the presence of three C4 gene copies in some of the t chromosomes and two copies in others. The enzymes distinguished 16 different haplotypes among the 33 strains tested. Based on their restriction fragment length patterns, the t strains could be divided into four groups with strains in each group more closely related to each other with respect to their C4-region genes than strains belonging to different groups. At least three of these four groups represent different branches of the evolutionary tree constructed for the t chromosomes. The C4-related genes of the chromosomes are in strong linkage disequilibrium with the class II genes of the H-2 complex. Typing for the Ss and Slp allotypes of C4 has revealed the presence of the Ss1 phenotype in two t strains and of the Slpa phenotype in one strain.
Golubić
M
M
Figueroa
F
F
Tosi
M
M
Klein
J
J
D000818:Animals; D003181:Complement C4 / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005796:Genes; D008040:Genetic Linkage; D051379:Mice; D008815:Mice, Inbred Strains; D011110:Polymorphism, Genetic
D003181:Complement C4; D004262:DNA Restriction Enzymes