Molecular cloning and characterization of the cDNA coding for hepatitis B virus surface antigen.
Hepatitis B virus surface antigen (HBsAg) mRNA has been enriched from a hepatoma cell line (PLC/PRF/5) by specific polysome immunoprecipitation and used for cDNA cloning. A HBsAg cDNA clone was identified by in situ hybridization with a cloned viral probe. It was characterized by restriction endonuclease mapping and DNA sequence analysis. Molecular hybridization of PLC/PRF/5 cellular DNA and RNA to [32P]-labeled HBsAg cDNA revealed the integration of at least six copies of the hepatitis B virus (HBV) DNA into the host genome and expression of three DNA species containing HBsAg-specific sequences. The possible role of HBV in the oncogenesis of primary hepatocellular carcinoma is discussed.
He
B G
BG
Melnick
J L
JL
Siddiqui
A
A
Robinson
W S
WS
Law
S W
SW
Lai
E C
EC
D006528:Carcinoma, Hepatocellular / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D005815:Genetic Code; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D008113:Liver Neoplasms; D009857:Oncogenes; D012333:RNA, Messenger / Q000235:genetics
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D012333:RNA, Messenger