The basolateral membrane of rat enterocyte: its purification from brush border contamination.
Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaCl2 to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low L-glucose permeability across basolateral membrane vesicles together with ATP-dependent sodium uptake was observed.
Orsenigo
M N
MN
Tosco
M
M
Esposito
G
G
Faelli
A
A
D000818:Animals; D002118:Calcium; D002462:Cell Membrane / Q000032:analysis* / Q000201:enzymology; D002498:Centrifugation; D005947:Glucose / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism; D012979:Sodium Radioisotopes; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D012979:Sodium Radioisotopes; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D002118:Calcium