title: Hormone dependency of human breast neoplasms cultured in vitro in the stem cell assay.
abstract: The stem cell soft agar culture system provides a biological tool with which to study tumor clones derived from heterogeneous tumor cell populations. For testing the feasibility of this approach in identifying hormone-responsive clones of mammary tumors, a study was done to determine whether estrogen deprivation or supplementation would alter colony formation in sequential breast neoplasms received in the Cell Kinetics Laboratory, The Pennsylvania State University. Single-cell suspensions obtained from 22 freshly excised breast neoplasms (20 malignant, 2 benign) were plated in soft agar with and without tamoxifen (Tam) (10(-7) M) with the use of serum-supplemented media and with and without 17 beta-estradiol (E2) (10(-8) M] under serum-free medium conditions. Twenty tumors successfully grew, producing 42 +/- 8 (mean +/- SEM) colonies in control dishes in the presence of serum and 27 +/- 5 in its absence (P less than .001). Tam inhibited colony formation in 78% of malignant tumors, whereas a stimulatory effect was observed with E2 in 94%. An increase in colony formation was induced by Tam in 4 tumors (2 benign and 2 malignant). The effects of E2 and Tam on tumor growth were not influenced by the estrogen and progesterone receptor status of the tumor. These preliminary results suggest that all tumors, irrespective of their receptor status, may contain clones of hormone-responsive cells.
authors.forename: A
authors.initials: A
authors.lastname: Manni
authors.forename: C
authors.initials: C
authors.lastname: Wright
authors.forename: M
authors.initials: M
authors.lastname: Pontari
authors.forename: P
authors.initials: P
authors.lastname: Feil
authors.forename: R
authors.initials: R
authors.lastname: Joehl
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000473:pathology; D000232:Adenofibroma / Q000473:pathology; D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000473:pathology* / Q000556:secondary; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005348:Fibrocystic Breast Disease / Q000473:pathology; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011959:Receptors, Estradiol / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D013629:Tamoxifen / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
chemical_list: D011959:Receptors, Estradiol; D011980:Receptors, Progesterone; D013629:Tamoxifen; D004958:Estradiol