title: Human leukocyte interferon inhibits human chorionic gonadotropin stimulated testosterone production by porcine Leydig cells in culture.
abstract: Pretreatment of primary porcine Leydig cell cultures with human leukocyte interferon suppressed the subsequent hCG-stimulated testosterone production in a dose-dependent manner, with an ED50 at 13 IU/ml. The treatment had no effect on hCG-binding to its receptor, and the inhibition of testosterone production was not abolished by 8Br-cAMP addition. The results indicate that the site of interferon action on hCG-stimulated testosterone production in primary cultures of porcine Leydig cells is located distal to cAMP formation.
authors.forename: M
authors.initials: M
authors.lastname: Orava
authors.forename: K
authors.initials: K
authors.lastname: Cantell
authors.forename: R
authors.initials: R
authors.lastname: Vihko
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000096:biosynthesis; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007700:Kinetics; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D013552:Swine; D013739:Testosterone / Q000096:biosynthesis*
chemical_list: D006063:Chorionic Gonadotropin; D007370:Interferon Type I; D015124:8-Bromo Cyclic Adenosine Monophosphate; D013739:Testosterone; D000242:Cyclic AMP