Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter.
In vitro expression of cDNA encoding bovine opsin is accomplished using the baculovirus expression vector system. Full-length opsin was synthesized which was recognized by poly- and monoclonal antisera raised against bovine rhodopsin. Upon infection with a recombinant virus, 1 x 10(6) insect cells produced up to 3 micrograms opsin. Incubation of the in vitro synthesized opsin with 11-cis retinal produced a hydroxylamine-stable, photosensitive pigment.
Janssen
J J
JJ
Department of Biochemistry, University of Nijmegen, The Netherlands.
van de Ven
W J
WJ
van Groningen-Luyben
W A
WA
Roosien
J
J
Vlak
J M
JM
de Grip
W J
WJ
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors; D007304:Insect Viruses / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017299:Rod Opsins
D005136:Eye Proteins; D011994:Recombinant Proteins; D017299:Rod Opsins; D004247:DNA