Ethanol oxidation by hydroxyl radicals: role of iron chelates, superoxide, and hydrogen peroxide.
Oxygen-derived free radicals such as the hydroxyl radical (.OH) have been shown to mediate the oxidation of ethanol by a variety of oxy radical-generating systems. Among these are microsomal electron transport systems (both intact and purified, reconstituted systems), the coupled oxidation of hypoxanthine or xanthine by xanthine oxidase, and the model iron-ascorbate system. The sequence of reactions leading to the oxy radical-dependent oxidation of ethanol as well as other hydroxyl radical-scavenging agents by these various systems is believed to proceed through the formation of a common intermediate, namely, hydrogen peroxide (H2O2), after dismutation of the superoxide anion radical (O2-.). The presence of iron, especially chelated iron, greatly enhances the production of .OH by serving as an oxidant for O2-. or a reductant for H2O2. Experiments were carried out to evaluate the role of iron, the chelating agent, O2-., and H2O2 in the oxidation of ethanol by a variety of in vitro systems (chemical, enzymatic, and intact membrane bound) that can produce oxy radicals via different mechanisms. The generation of .OH by all the systems studied was sensitive to catalase, which indicates that H2O2 is the precursor of .OH. Superoxide radical appears to be the reducing agent in the hypoxanthine-xanthine oxidase system, indicating an iron-catalyzed Haber-Weiss reaction. In the ascorbate, reductase, and microsomal systems, superoxide radical does not appear to be the reducing agent. However, superoxide radical probably is the precursor of H2O2. While iron plays an important role in the production of .OH by the various systems, the effect of iron depends on the nature of the iron chelate.(ABSTRACT TRUNCATED AT 250 WORDS)
Feierman
D E
DE
Winston
G W
GW
Cederbaum
A I
AI
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism*; D005290:Ferric Compounds / Q000494:pharmacology; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives / Q000378:metabolism; D008862:Microsomes, Liver / Q000502:physiology; D009251:NADPH-Ferrihemoprotein Reductase / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D013481:Superoxides / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000494:pharmacology
D005290:Ferric Compounds; D005609:Free Radicals; D007502:Iron Chelating Agents; D013481:Superoxides; D000431:Ethanol; C014632:2-keto-4-methylthiobutyric acid; D004492:Edetic Acid; D008715:Methionine; D006861:Hydrogen Peroxide; D002374:Catalase; D014969:Xanthine Oxidase; D009251:NADPH-Ferrihemoprotein Reductase; C019179:Fe(III)-EDTA; D001205:Ascorbic Acid