title: A temperature-sensitive Chinese hamster ovary cell mutant pleiotropically defective in protein export.
abstract: We have developed a new selection procedure for mammalian cell mutants defective in protein export by the use of diphtheria toxin, and devised a new screening method for defective protein secretion using nitrocellulose membranes. By the combination of these procedures, we have isolated a temperature-sensitive mutant clone of Chinese hamster ovary cells which shows a pleiotropic defect in protein export. This mutant, designated DS28-6, is temperature-sensitive for growth. Secretion of a series of proteins is markedly inhibited at the non-permissive temperature. These proteins seem to be normally synthesized and accumulated within the cell at the non-permissive temperature and secreted upon shift down to the permissive temperature. When this mutant is infected with vesicular stomatitis virus, oligosaccharide processing of G-protein is arrested at an endoglycosidase-H-sensitive stage at the non-permissive temperature. The lesion of this mutant appears to be in the endoplasmic reticulum or the cis Golgi or both.
authors.forename: A
authors.initials: A
authors.lastname: Nakano
authors.forename: M
authors.initials: M
authors.lastname: Nishijima
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: Y
authors.initials: Y
authors.lastname: Akamatsu
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D009154:Mutation; D010053:Ovary; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D011506:Proteins; D014764:Viral Proteins