5-Hydroxytryptamine1A receptors are linked to a Gi-adenylate cyclase complex in rat hippocampus.
The ability of 5-hydroxytryptamine (5-HT) and the 5-HT1A selective agonist 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) to modulate adenylate cyclase activity was measured in rat hippocampus. In vitro ADP ribosylation of GTP-binding proteins by pertussis toxin in this tissue abolished both 5-HT- and 8-OH-DPAT-induced inhibition of forskolin-stimulated adenylate cyclase activity. These findings indicate that 5-HT1A receptors are linked a pertussis-sensitive Gi protein in rat hippocampus.
Harrington
M A
MA
Department of Neurology, Stanford University Medical Center, CA 94305.
Oksenberg
D
D
Peroutka
S J
SJ
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000032:analysis*; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D037342:Pertussis Toxin; D015221:Potassium Channels / Q000187:drug effects; D051381:Rats; D011985:Receptors, Serotonin / Q000502:physiology*; D013764:Tetrahydronaphthalenes / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D015221:Potassium Channels; D011985:Receptors, Serotonin; D013764:Tetrahydronaphthalenes; D010566:Virulence Factors, Bordetella; D005576:Colforsin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases