Interaction with mitochondrial membranes of a synthetic peptide with a sequence common to extra peptides of mitochondrial precursor proteins.
A hepta-peptide, Arg-Leu-Leu-Pro-Ser-Leu-Gly, which has a sequence involved in the extra peptides of mitochondrial proteins, was synthesized chemically. The peptide was found to bind specifically to mitochondria, but not to microsomes. The binding was blocked by pretreatment of mitochondria with trypsin but was not affected by the presence of apocytochrome c. The synthetic peptide inhibited the binding to mitochondria of the precursor protein of ATPase inhibitor, which was synthesized in vitro, but did not inhibit that of the precursor of the 9 K stabilizing factor, which has an entirely different extra-peptide sequence. The peptide also did not inhibit the binding of apocytochrome c. These results suggest the existence of a common protein receptor on mitochondrial membranes that facilitates entrance of a group of mitochondrial precursor proteins, including pre-ATPase inhibitor.
Yoshida
Y
Y
Hashimoto
T
T
Kimura
H
H
Sakakibara
S
S
Tagawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003574:Cytochrome c Group / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D012440:Saccharomyces / Q000648:ultrastructure; D014357:Trypsin / Q000378:metabolism; D000097795:ATPase Inhibitory Protein
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003574:Cytochrome c Group; D010455:Peptides; D011498:Protein Precursors; D011506:Proteins; D014357:Trypsin; D000097795:ATPase Inhibitory Protein