Photoaffinity labeling of atrial natriuretic factor receptors of rat kidney cortex plasma membranes.
Synthetic rat atrial natriuretic factor (ANF) was derivatized with the N-hydroxysuccinimide ester of [125I]iodoazidosalicylic acid to yield a radioactive photoaffinity probe. Incubation of purified plasma membranes from rat kidney cortex with this photoaffinity probe resulted in the specific labeling of a 140-kDa glycoprotein. The photoaffinity labeling of this protein was inhibited by ANF but not by reduced and alkylated ANF nor by other unrelated peptides. A 140-kDa band was also specifically labeled in liver plasma membranes but not in adipocyte plasma membranes. These observations suggest strongly that the 140-kDa glycoprotein is the ANF receptor.
Yip
C C
CC
Laing
L P
LP
Flynn
T G
TG
D000273:Adipose Tissue / Q000166:cytology; D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor; D001386:Azides / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004229:Dithiothreitol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000378:metabolism; D007460:Iodoacetamide / Q000494:pharmacology; D007672:Kidney Cortex / Q000166:cytology*; D008099:Liver / Q000166:cytology; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism; D010777:Photochemistry; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012459:Salicylates / Q000378:metabolism; D013552:Swine
D000345:Affinity Labels; D001386:Azides; D011956:Receptors, Cell Surface; D012459:Salicylates; C472610:atrial natriuretic peptide, rat; D009320:Atrial Natriuretic Factor; C046019:4-azidosalicylic acid N-hydroxysuccinimide ester; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D017461:Receptors, Atrial Natriuretic Factor; D004229:Dithiothreitol; D007460:Iodoacetamide