Radioimmunoassay for detection of VP1 specific neutralizing antibodies of foot and mouth disease virus.
A solid-phase radioimmunoassay was developed for the detection of antibodies against a specific region of the VP1 protein of the A24 and 01 serotypes of foot and mouth disease virus. The antibody titers from the radioimmunoassay showed a positive correlation with neutralizing antibody titers determined by a mouse protection assay. The specificity of the assay resides in the peptide used as antigen. The assay is rapid, reproducible and does not require the use of whole virions.
Patzer
E J
EJ
Jackson
M L
ML
Moore
D M
DM
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D005537:Aphthovirus / Q000276:immunology*; D006168:Guinea Pigs; D051379:Mice; D011863:Radioimmunoassay; D014764:Viral Proteins / Q000276:immunology*; D015678:Viral Structural Proteins
D000914:Antibodies, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins