[Ingestion of soybean epoxide oil. Effects on monooxygenases, epoxide hydrolase and activities of UDP glucuronosyltransferases in hepatic microsomes of the rat].
The effect of peroxidized soybean oil in the diet of male Wistar rats was studied on hepatic drug metabolizing enzymes and their phenobarbital induction and compared to that of natural soybean diet in the same conditions. No hepatomegaly or increase in serum transaminases occurred, however growth was inhibited after ingestion of peroxidized soybean oil. In addition, the protein biosynthesis of epoxide hydrase determined by immunochemistry was largely stimulated by this treatment; but the corresponding activity measured with benzo(a)pyrene 4-5 oxide as a substrate was increased in weaker proportions. This induction was limited to epoxide hydrolase only, since the enzymes of phase one were not affected and UDP glucuronosyltransferase activities toward group I substrates were randomly activated. The induction of epoxide hydrolase may affect only one or several isoforms of the membrane enzyme which are not necessarily specific to benzo(a)pyrene 4-5 oxide activity determination of the enzyme.
Mounié
J
J
Formation de Biochemie pharmacologique de l'Université de Bourgogne.
Faye
B
B
Goudonnet
H
H
Magdalou
J
J
Champion
L
L
Escousse
A
A
Siest
G
G
Truchot
R C
RC
D000818:Animals; D004032:Diet; D004851:Epoxide Hydrolases / Q000378:metabolism*; D004852:Epoxy Compounds / Q000494:pharmacology*; D004988:Ethers, Cyclic / Q000494:pharmacology*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013024:Soybean Oil / Q000494:pharmacology*
D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D010938:Plant Oils; D013024:Soybean Oil; D014453:Glucuronosyltransferase; D004851:Epoxide Hydrolases
Ingestion d'huile de soja époxydée. Effet sur les monooxygénases, l'époxyde hydrolase et les activités UDP glucuronosyltransférases des microsomes hépatiques chez le rat.