Human B cell responsiveness to B cell growth factor after activation by phorbol ester and monoclonal anti-mu antibody.
The effect of phorbol ester on human B cell activation was examined. Picomolar to nanomolar concentrations of phorbol ester induced a high level of proliferation in small IgM-positive B cells isolated from peripheral blood by fluorescence-activated cell sorting. The addition of optimal doses of anti-mu antibody resulted in enhanced proliferation of phorbol ester-activated B cells. The addition of B cell growth factor (BCGF) to phorbol ester-activated B cells also resulted in a dose-dependent synergistic effect and maximal enhancement on day 3. BCGF activity could be absorbed with either phorbol ester- or anti-mu-activated B cells, but not with resting B cells, thus confirming the induction of functional BCGF receptor expression. Cell proliferation was not necessary for the induction of functional BCGF receptors. Phorbol ester was a more efficient inducer of BCGF receptor expression than was anti-mu antibody; gamma-interferon treatment had no effect. BCGF enhanced transferrin receptor expression by phorbol ester-activated B cells. The results suggest that phorbol ester-activated small B cells can be used to monitor BCGF activity, and this synergistic combination may be useful in establishing BCGF-dependent B cell clones in culture.
Suzuki
T
T
Butler
J L
JL
Cooper
M D
MD
D000042:Absorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004357:Drug Synergism; D006041:Goats; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007148:Immunoglobulin mu-Chains / Q000276:immunology*; D007371:Interferon-gamma / Q000502:physiology; D015847:Interleukin-4; D007399:Interphase / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000502:physiology*; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D010704:Phorbols / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D011971:Receptors, Immunologic / Q000096:biosynthesis; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D007143:Immunoglobulin Heavy Chains; D007148:Immunoglobulin mu-Chains; D008222:Lymphokines; D008934:Mitogens; D010704:Phorbols; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin; D015847:Interleukin-4; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate