In vitro immunization for antibody production against tetanus toxin and toxoid. 3. Recombinant gamma interferon and indomethacin support specific antibody production induced by antigen-pulsed immobilized monocytes.
Specific antibody production against Tetanus Toxin by in vitro cultured human peripheral blood lymphocytes may be enhanced, when monocytes were immobilized onto FCS-coated culture plates and then pulsed with Tetanus Toxoid. Using this model several immunomodulators were tested for their influence on in vitro immunization efficiency. A combination of recombinant gamma interferon and indomethacin added during antigen presentation to lymphocytes, was able to potentiate specific antibody response.
Jahn
S
S
Department of Medicine Charité, Humboldt-University, Berlin.
Grunow
R
R
Kiesing
S T
ST
Volk
H D
HD
von Baehr
R
R
D000917:Antibody Formation / Q000187:drug effects*; D000918:Antibody Specificity / Q000187:drug effects; D000938:Antigen-Presenting Cells / Q000276:immunology; D006801:Humans; D007213:Indomethacin / Q000008:administration & dosage*; D007371:Interferon-gamma / Q000008:administration & dosage*; D007376:Interleukin-2 / Q000008:administration & dosage; D011994:Recombinant Proteins / Q000008:administration & dosage*; D013744:Tetanus Toxin / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
D007376:Interleukin-2; D011994:Recombinant Proteins; D013744:Tetanus Toxin; D013745:Tetanus Toxoid; D007371:Interferon-gamma; D007213:Indomethacin