Cell morphology, proliferation and collagen synthesis of human fibroblasts cultured on sepiolite-collagen complexes.
The growth and morphology as well as collagen biosynthesis of human fibroblasts obtained and cultured on sepiolite-collagen complexes have been studied. No differences on cell morphology and growth properties nor collagen synthesis were observed when compared with standard culture substrates. The type I/type III ratio of biosynthesized collagen by fibroblasts cultured on sepiolite-collagen complexes was about 5-6 with no difference when compared to control conditions. This normal behavior was also observed for the type I/type III procollagens. According to these studies the sepiolite-collagen complexes do not modify the studied features of the fibroblasts.
Olmo
N
N
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad Complutense, Madrid, Spain.
Lizarbe
M A
MA
Turnay
J
J
Müller
K P
KP
Gavilanes
J G
JG
D001672:Biocompatible Materials; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000145:classification; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D017633:Magnesium Silicates; D008903:Minerals
D001672:Biocompatible Materials; D017633:Magnesium Silicates; D008903:Minerals; D003094:Collagen; C001671:magnesium trisilicate