Proteolysis of mitochondria in reticulocytes during maturation is ubiquitin-dependent and is accompanied by a high rate of ATP hydrolysis.
The ATP-dependent breakdown of mitochondria-containing stroma proceeds via the ubiquitin-requiring pathway. The proteolysis is linked to a large ATP-cleaved consumption amounting to 1 ATP per peptide bond or more. Proteins of mitochondria-containing stroma are much better substrates of ATP-ubiquitin-dependent proteolysis than heat-denatured ones. Hemin suppresses both proteolysis and ATP hydrolysis.
Rapoport
S
S
Dubiel
W
W
Müller
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D005033:Ethylmaleimide / Q000494:pharmacology; D006427:Hemin / Q000494:pharmacology; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006868:Hydrolysis; D008274:Magnesium / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000648:ultrastructure*; D014452:Ubiquitins / Q000378:metabolism*
D006609:High Mobility Group Proteins; D014452:Ubiquitins; D006427:Hemin; D000255:Adenosine Triphosphate; D008274:Magnesium; D005033:Ethylmaleimide