title: Cyclic-AMP-dependent protein kinase type II is associated with the Golgi complex and with centrosomes.
abstract: The subcellular distribution of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) in epithelial and fibroblastic cells was determined by indirect immunofluorescence microscopy. In interphase cells both regulatory (R II) and catalytic (C) subunits were concentrated in a perinuclear area. By comparison of the R II distribution with the location of a bona fide Golgi membrane constituent, this area was identified as the Golgi complex. The cytochemical localization of R II was confirmed by subcellular fractionation. In addition, cAMP-dPK II was associated with microtubule-organizing centers, in particular with mitotic spindle poles. These distributions of cAMP-dPK II probably represent important factors in mediating the effects of cAMP on basic cellular activities ranging from secretion and proliferation to cell shape and motility.
authors.forename: E A
authors.initials: EA
authors.lastname: Nigg
authors.forename: G
authors.initials: G
authors.lastname: Schäfer
authors.forename: H
authors.initials: H
authors.lastname: Hilz
authors.forename: H M
authors.initials: HM
authors.lastname: Eppenberger
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D002458:Cell Fractionation; D002460:Cell Line; D000242:Cyclic AMP / Q000494:pharmacology; D004848:Epithelium; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000201:enzymology*; D007399:Interphase; D008938:Mitosis; D011494:Protein Kinases / Q000032:analysis* / Q000276:immunology; D008941:Spindle Apparatus / Q000201:enzymology*
chemical_list: D000906:Antibodies; D000242:Cyclic AMP; D011494:Protein Kinases