title: Lectin (UEA-1) reaction of capillary endothelium with reference to permeability in autopsied cases of cerebral infarction.
abstract: The relationship between endothelial reactivity to Ulex europaeus agglutinin-1 (UEA-1) and the permeability of the vascular wall in human autopsied cases of cerebral infarction was studied. Sections from the cerebral cortex were reacted with horseradish peroxidase UEA-1 to demonstrate the surface membrane of endothelial cells. Albumin in the neuropil of sections was demonstrated for the estimation of increased vascular permeability. The results showed that endothelial reactivity to UEA-1 was reduced in cases where death had occurred 3 to 5 days after onset of cerebral infarction. Reactivity was also diminished in cases where death had occurred after 13 and 25 days; these cases showed fresh ischemic lesions caused by re-attacks of infarction. Albumin extravasation into the neuropil was demonstrated in these intermediate cases. Chronic cases, dying after more than 52 days, showed no reduction of endothelial reactivity to UEA-1 and no albumin extravasation was proved. It was concluded that UEA-1 can be employed as a useful morphological marker for evaluation of endothelial function and vascular permeability.
authors.affiliation: Second Department of Pathology, Kinki University School of Medicine, Osaka, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Kadota
authors.forename: K
authors.initials: K
authors.lastname: Tanji
authors.forename: S
authors.initials: S
authors.lastname: Nishida
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: S
authors.initials: S
authors.lastname: Hiruma
authors.forename: Y
authors.initials: Y
authors.lastname: Enomoto
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.forename: F
authors.initials: F
authors.lastname: Akai
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001344:Autopsy; D002199:Capillary Permeability; D002544:Cerebral Infarction / Q000378:metabolism / Q000473:pathology*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins; D008297:Male; D037121:Plant Lectins; D012709:Serum Albumin / Q000032:analysis
chemical_list: D037102:Lectins; D037121:Plant Lectins; D012709:Serum Albumin; C036850:Ulex europaeus lectins