Murine cutaneous leishmaniasis: susceptibility correlates with differential expansion of helper T-cell subsets.
BALB/c mice develop fatal illness following infection with Leishmania major despite expansion of helper L3T4+ T cells in the draining lymph nodes and spleen. Healer mice, either genetically resistant C57BL/6 or BALB/c that have been pretreated with monoclonal antibody GK 1.5, also develop expanded numbers of L3T4+ T cells at the time of healing. Lymph node cells from healer mice produce gamma-interferon in vitro and message for gamma-interferon can be recovered from the lymph nodes during healing in vivo. Conversely, cells harvested from non-healer mice during the course of infection produce minimal gamma-interferon in vitro and have little message for gamma-interferon detectable in vivo. When the same Northern blots are hybridized for IL-4, large amounts of IL-4 message are detected only in cells from non-healer mice. The data are consistent with the expansion of type 1 helper cells (Th1) during healing and type 2 helper cells (Th2) during progressive leishmania infection.
Locksley
R M
RM
Department of Medicine, University of California San Francisco Medical Center 94143.
Heinzel
F P
FP
Sadick
M D
MD
Holaday
B J
BJ
Gardner
K D
KD
D000818:Animals; D004198:Disease Susceptibility; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007896:Leishmaniasis / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification* / Q000276:immunology
D012333:RNA, Messenger; D007371:Interferon-gamma