The human cellular abl gene product in the chronic myelogenous leukemia cell line K562 has an associated tyrosine protein kinase activity.
Three antisera against the mouse v-abl gene product were used to identify two potential human c-abl gene products in the chronic myelogenous leukemia cell line K562. Two antipeptide sera were generated in rabbits using the predicted amino acid sequence of the mouse v-abl gene product. One antiserum was made against a polypeptide overlapping the in vivo tyrosine phosphorylation site of murine P120gag-abl and what is believed to be a homologous tyrosine phosphorylation site of the predicted normal human c-abl gene product (v-abl 263-280). The second antipeptide serum, abl 389-403, was generated against a predicted hydrophilic peptide of the v-abl gene product. Immunoprecipitation from K562 cells metabolically labeled with [32P]orthophosphate by a mouse tumor regressor and abl 389-403 antipeptide sera detected two proteins of 190,000 and 240,000 Da. Both proteins were labeled primarily at serine and, to a much lesser extent, at tyrosine residues. Immune complex kinase assays using conditions that allow the tyrosine phosphorylation of P120gag-abl showed that in vitro phosphorylation of P190 and P240 occurs primarily at tyrosine residues. The detection of these enzymatically active human c-abl gene products is a rare observation which may be in part attributed to the c-abl gene translocation from chromosomes 9 to 22 occurring in the vast majority of chronic myelogenous leukemia patients.
Kloetzer
W
W
Kurzrock
R
R
Smith
L
L
Talpaz
M
M
Spiller
M
M
Gutterman
J
J
Arlinghaus
R
R
D000011:Abelson murine leukemia virus / Q000235:genetics / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D006801:Humans; D007122:Immunoelectrophoresis; D009052:Leukemia Virus, Murine / Q000276:immunology*; D007951:Leukemia, Myeloid / Q000235:genetics*; D051379:Mice; D009363:Neoplasm Proteins / Q000276:immunology; D009857:Oncogenes; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics* / Q000276:immunology; D011505:Protein-Tyrosine Kinases; D014764:Viral Proteins / Q000276:immunology
D009363:Neoplasm Proteins; D014764:Viral Proteins; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases