Anti-morphine anti-idiotypic antibodies. Opiate receptor binding and isolated tissue responses.
Anti-idiotypic antibodies which recognize the opiate receptor were generated in guinea pigs following immunization against purified rabbit anti-morphine antibodies. The anti-idiotypic antibodies produced a concentration-dependent inhibition of [3H]naloxone binding to opiate receptors in a membranous mouse brain preparation. Saturation analysis indicated that the antibodies produced a non-competitive inhibition of naloxone binding. The ability of the antibodies to interact with biological systems was investigated in in vitro systems. In both the isolated guinea pig ileal longitudinal muscle and mouse vas deferens, the antibodies produced a concentration-dependent, opiate agonist-like action. The anti-morphine anti-idiotypic antibodies appear to interact specifically with the opiate receptor and may serve as useful tools in characterization of this receptor system.
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D001921:Brain / Q000378:metabolism; D006168:Guinea Pigs; D007082:Ileum / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009020:Morphine / Q000276:immunology* / Q000378:metabolism; D009270:Naloxone / Q000378:metabolism; D011817:Rabbits; D011957:Receptors, Opioid / Q000378:metabolism*; D014649:Vas Deferens / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes; D011957:Receptors, Opioid; D009270:Naloxone; D009020:Morphine