title: Effects of acetaminophen and nonsteroidal anti-inflammatory drugs on progesterone production by porcine granulosa cells in vitro.
abstract: We investigated the effects of a group of pharmaceutical agents commonly ingested by reproductive-aged women, acetaminophen and the nonsteroidal anti-inflammatory drugs (NSAID), on progesterone (P) production by cultures of highly differentiated porcine granulosa cells. These compounds were added to cultures over a dose range of 10(-8) to 10(-5) M and P, and cell protein was measured after 24 hours. P production was suppressed by acetaminophen, fenoprofen, and sulindac to a maximum of 81%, 76%, and 71% of control, respectively. P production was enhanced by butazolidin at all doses tested to a maximum of 140% of control. Granulosa cell protein was suppressed by butazolidin and salicylic acid to a maximum of 81% of controls. These data imply that acetaminophen and several NSAID have the potential for clinical reproductive toxicity with differing individual effects on reproductive tract tissues, suggesting further selective testing in vivo.
authors.affiliation: Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: A F
authors.initials: AF
authors.lastname: Haney
authors.forename: S F
authors.initials: SF
authors.lastname: Hughes
authors.forename: C L
authors.initials: CL
authors.lastname: Hughes
mesh_terms: D000082:Acetaminophen / Q000009:adverse effects*; D000704:Analysis of Variance; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D002478:Cells, Cultured; D015925:Cryopreservation; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*; D013552:Swine
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000082:Acetaminophen; D011374:Progesterone