title: Plasmid-borne Tn5 insertion mutation resulting in accumulation of gentisate from salicylate.
abstract: Plasmid-borne Tn5 insertion mutants of a Pseudomonas species which accumulated 2,5-dihydroxybenzoate (gentisate) following growth on 2-hydroxybenzoate (salicylate) were obtained from a pool of mutants that were unable to grow on naphthalene. One such mutant was characterized further. The ability of this mutant to oxidize gentisate was 100-fold less than the ability of a Nah+ Sal+ strain harboring the unmutagenized plasmid, although both strains oxidized and grew on salicylate. These bacteria were presumably able to metabolize salicylate via catechol, since they possessed an inducible, plasmid-encoded catechol 2,3-dioxygenase. Our results suggest that there is an alternate, plasmid-encoded route of salicylate degradation via gentisate and that some plasmid-associated relationship between this pathway and naphthalene oxidation exists.
authors.forename: D J
authors.initials: DJ
authors.lastname: Monticello
authors.forename: D
authors.initials: D
authors.lastname: Bakker
authors.forename: M
authors.initials: M
authors.lastname: Schell
authors.forename: W R
authors.initials: WR
authors.lastname: Finnerty
mesh_terms: D050557:Catechol 2,3-Dioxygenase; D004251:DNA Transposable Elements; D049308:Dioxygenases; D005841:Gentisates / Q000378:metabolism*; D009154:Mutation; D010101:Oxygen Consumption; D010105:Oxygenases / Q000235:genetics; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D012459:Salicylates / Q000378:metabolism*; D020156:Salicylic Acid; D012988:Soil Microbiology
chemical_list: D004251:DNA Transposable Elements; D005841:Gentisates; D012459:Salicylates; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D020156:Salicylic Acid