Inhibition of lymphocyte proliferation by synthetic peptides homologous to human plasma apolipoproteins B and E.
Apolipoproteins B and E of the human plasma lipoproteins are known inhibitors of lymphocyte proliferation. In this report, two synthetic peptide amides, apoB3358-3372 and apoE141-155, showed a dose-dependent inhibition of both the murine mixed lymphocyte culture reaction and the anti-T3 induced proliferation of lymphocytes. Their structures and antiproliferative potencies were similar to that of the heptadecapeptide CKS-17, a consensus peptide of a highly conserved region among HTLV-I, -II and C-type human retroviral proteins. SP-9-2-amide, a peptide homologous to the amino-terminal half of CKS-17, also suppressed lymphocyte activation. In contrast, a peptide homologous to the gp41 protein of HTLV-III that is sequence related to CKS-17 (approximately 35% homology) showed little antiproliferative activity. Neurotensin, a control peptide, showed no activity. The results suggest that a basic tetrapeptide sequence common to CKS-17-amide, SP-9-2, apoB3358-3372 and apoE141-155, but not HTLV-III-amide may account, in part, for the antiproliferative activities of these peptides.
Cardin
A D
AD
Merrell Dow Research Institute, Cincinnati, OH 45215.
Bowlin
T L
TL
Krstenansky
J L
JL
D001055:Apolipoproteins B / Q000031:analogs & derivatives* / Q000494:pharmacology; D001057:Apolipoproteins E / Q000031:analogs & derivatives* / Q000494:pharmacology; D001665:Binding Sites; D002455:Cell Division / Q000187:drug effects; D006678:HIV / Q000032:analysis; D006493:Heparin / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D013936:Thymidine / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis
D001055:Apolipoproteins B; D001057:Apolipoproteins E; D014764:Viral Proteins; D006493:Heparin; D013936:Thymidine