Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells.
The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (psrc-11). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60src, and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.
Chang
C C
CC
Trosko
J E
JE
Kung
H J
HJ
Bombick
D
D
Matsumura
F
F
D000818:Animals; D002450:Cell Communication; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002999:Clone Cells; D004262:DNA Restriction Enzymes; D005796:Genes; D007365:Intercellular Junctions / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D011493:Protein Kinase C; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology
D014500:Uracil Nucleotides; D014764:Viral Proteins; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src); D011493:Protein Kinase C; D004262:DNA Restriction Enzymes