beta-Adrenergic and cholinergic receptors in hypertension-induced hypertrophy.
Perinephritic hypertension was produced in dogs by wrapping one kidney with silk and removing the contralateral kidney 1 week later. Mean arterial pressure rose from 104 +/- 3 to 156 +/- 11 mm Hg, while left ventricular free wall weight, normalized for body weight, was increased by 49%. Muscarinic, cholinergic receptor density measured with [3H]-quinuclidinyl benzilate, fell in hypertensive left ventricles (181 +/- 19 fmol/mg, n = 6; p less than 0.01) as compared with that found in normal left ventricles (272 +/- 16 fmol/mg, n = 8), while receptor affinity was not changed. The beta-adrenergic receptor density, measured by binding studies with [3H]-dihydroalprenolol, rose in the hypertensive left ventricles (108 +/- 10 fmol/mg, n = 7; p less than 0.01) as compared with that found in normal left ventricles (68.6 +/- 5.2 fmol/mg, n = 15), while beta-adrenergic receptor affinity decreased in the hypertensive left ventricles (10.4 +/- 1.2 nM) compared with that found in the normal left ventricles (5.0 +/- 0.7 nM). Plasma norepinephrine levels were similar in the two groups, but myocardial norepinephrine levels were depressed (p less than 0.05) in dogs with hypertension. Moderate left ventricular hypertrophy induced by long-term aortic banding in dogs resulted in elevations in beta-adrenergic receptor density (115 +/- 14 fmol/mg) and decreases in affinity (10.4 +/- 2.2 nM) similar to those observed in the dogs with left ventricular hypertrophy induced by hypertension. Thus, our results suggest that perinephritic hypertension in the dog induces divergent effects on cholinergic and beta-adrenergic receptor density. The increased beta-adrenergic receptor density and decreased affinity may be a characteristic of left ventricular hypertrophy rather than hypertension.
Vatner
D E
DE
Kirby
D A
DA
Homcy
C J
CJ
Vatner
S F
SF
D000818:Animals; D001835:Body Weight; D006332:Cardiomegaly / Q000209:etiology / Q000378:metabolism*; D004082:Dihydroalprenolol / Q000378:metabolism; D004285:Dogs; D005260:Female; D006978:Hypertension, Renovascular / Q000150:complications*; D008297:Male; D009392:Nephrectomy; D009929:Organ Size; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000032:analysis*; D011950:Receptors, Cholinergic / Q000032:analysis*; D014316:Tritium
D011943:Receptors, Adrenergic, beta; D011950:Receptors, Cholinergic; D014316:Tritium; D004082:Dihydroalprenolol; D011813:Quinuclidinyl Benzilate