Fractionation of red diamond rattlesnake (Crotalus ruber ruber) venom: protease, phosphodiesterase, L-amino acid oxidase activities and effects of metal ions and inhibitors on protease activity.
Crotalus ruber ruber venom contains several different proteases, and the proteolytic activity of the crude venom is 6-15 times greater in adult than in juvenile venom. Venom samples were assayed for proteolytic, phosphodiesterase, L-amino acid oxidase and elastinase-like activities and were subjected to gel filtration on BioGel P-100. Two major size classes of proteases were resolved (mol. wt 67,000 and 20,500). EDTA, N-ethylmaleimide (N-EM) and 1,10-phenanthroline inhibited proteolytic activity of crude venom, and EDTA, Zn2+ and Cu2+ inhibited proteolytic activity of the fractionated venom.
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D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D002850:Chromatography, Gel; D003435:Crotalid Venoms / Q000032:analysis*; D050830:L-Amino Acid Oxidase; D008670:Metals / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000032:analysis*; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D011480:Protease Inhibitors / Q000494:pharmacology*
D003435:Crotalid Venoms; D008670:Metals; D011480:Protease Inhibitors; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; D010727:Phosphoric Diester Hydrolases; D010447:Peptide Hydrolases