Effect of glucose on induction of delta-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead.
In the present work we have been able to demonstrate that phenobarbital and lead exert an inducing effect on the biosynthesis of delta-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the induction effect produced by phenobarbital in this in vitro system. Glucose inhibits the induction of delta-aminolevulinic acid synthase and ferrochelatase. This repression effect can be reversed with increasing concentrations of dibutyryl cyclic AMP. No glucose effect was observed on the phenobarbital- and lead-mediated inductions of cytochrome P-450. he present results add more experimental evidence to support the concept that the last enzyme of the heme pathway is inducible, and as such may have a significant role in regulatory mechanisms of porphyrin and heme biosynthesis.
Cánepa
E T
ET
Llambías
E B
EB
Grinstein
M
M
D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D005294:Ferrochelatase / Q000096:biosynthesis*; D005947:Glucose / Q000494:pharmacology*; D006420:Hemeproteins / Q000096:biosynthesis*; D007328:Insulin / Q000494:pharmacology; D007854:Lead / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008190:Lyases / Q000096:biosynthesis*; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013806:Theophylline / Q000494:pharmacology
D006420:Hemeproteins; D007328:Insulin; D007854:Lead; D003994:Bucladesine; D003577:Cytochrome P-450 Enzyme System; D003513:Cycloheximide; D013806:Theophylline; D000624:5-Aminolevulinate Synthetase; D008190:Lyases; D005294:Ferrochelatase; D005947:Glucose; D004837:Epinephrine; D010634:Phenobarbital