The human vasopressin gene is linked to the oxytocin gene and is selectively expressed in a cultured lung cancer cell line.
The human genes for prepro-arginine-vasopressin-neurophysin II (prepro-AVP-NPII) and prepro-oxytocin-neurophysin I (prepro-OT-NPI) were cloned from a human genomic library and the nucleotide sequence of both genes was determined. The two genes are similar in their intron-exon structure, linked together with 12 kilobases intervening, and transcribed from opposite DNA strands. A human small cell lung cancer cell line, H378, produces significant quantities of pre-pro-AVP-NPII mRNA using a transcription unit predicted from the genomic DNA sequence. Despite the proximity of the actively transcribed prepro-AVP-NPII gene, transcription of prepro-OT-NPI is not detected in this cell line.
Sausville
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Carney
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Battey
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D000595:Amino Acid Sequence; D001127:Arginine Vasopressin / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D008040:Genetic Linkage; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D009481:Neurophysins / Q000235:genetics*; D010121:Oxytocin / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D014667:Vasopressins / Q000235:genetics*
D009481:Neurophysins; D011498:Protein Precursors; D014667:Vasopressins; D001127:Arginine Vasopressin; D010121:Oxytocin; D004262:DNA Restriction Enzymes