Physiological concentrations of atrial natriuretic factor stimulate human erythroid progenitors in vitro.
Human alpha-ANF[1-28] potentiated erythroid colony formation up to four-fold in cultures containing erythropoietin. Both early and late erythroid precursor cells responded to alpha-ANF[1-28] [0.032 to 1 nM] in a dose dependent fashion. Removal of T lymphocytes and macrophages which have been shown to modulate erythropoiesis did not abolish the stimulatory effect. All major circulatory forms of ANF (alpha-ANF[1-28], alpha-ANF[4-28] and alpha-ANF[5-28]) had potent erythropoietic activity. These results indicate that concentrations of ANF reached during hypoxia stimulate erythroid progenitor cells in the presence of erythropoietin.
Niskanen
E
E
Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville 22908.
Baertschi
A J
AJ
D009320:Atrial Natriuretic Factor / Q000502:physiology*; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004921:Erythropoietin / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D008264:Macrophages / Q000166:cytology; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000166:cytology
D006454:Hemoglobins; C472955:NPPA protein, human; D004921:Erythropoietin; D009320:Atrial Natriuretic Factor