Molecular cloning of integrated proviral DNA of bovine leukaemia virus from virus-producing foetal lamb kidney cells.
Over 90% of the total viral information together with the adjacent 5' cellular sequences were cloned in the lambdoid spi selection vector lambda-2558 by taking advantage of the unique EcoRI restriction site very close to the 3' long terminal repeat. Of the fifteen isolated recombinant phages with viral inserts, one has been propagated and its DNA isolated and subjected to preliminary restriction endonuclease analysis. The proviral insert has been found to be almost identical to the unintegrated proviral DNA reported by Kashmiri et al. (1984).
Nyakatura
G
G
Jantschak
J
J
Nötzel
U
U
Prösch
S
S
Rosenthal
S
S
Rosenthal
H A
HA
D000818:Animals; D010582:Bacteriophage lambda; D002460:Cell Line; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007668:Kidney; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D012756:Sheep; D014776:Virus Cultivation
D004279:DNA, Viral; D004262:DNA Restriction Enzymes