Cloning of the A gene of bacteriophage Mu and purification of its product, the Mu transposase.
The bacteriophage Mu transposase (the Mu A gene product), which is absolutely required for both integration of Mu and replicative transposition during the lytic cycle, has been overproduced by cloning the gene on a plasmid under the control of the phage lambda PL promoter. The protein has been purified to near homogeneity from the lysate of heat-induced cells of a strain carrying the plasmid. The purified protein is active as judged by its ability to complement Mu A- cell extracts for supporting Mu transposition in a cell-free reaction.
Craigie
R
R
Mizuuchi
K
K
D010582:Bacteriophage lambda / Q000235:genetics; D010583:Bacteriophage mu / Q000201:enzymology / Q000235:genetics*; D002845:Chromatography; D003001:Cloning, Molecular; D004279:DNA, Viral; D004926:Escherichia coli; D005814:Genes, Viral; D009713:Nucleotidyltransferases / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids; D019895:Transposases
D004279:DNA, Viral; D009713:Nucleotidyltransferases; D019895:Transposases