title: Effects of altered Ca2+ and Mg2+ concentrations on proliferation and functional differentiation of the clonal insulin-producing cells RINm5F.
abstract: Effects of calcium and magnesium on proliferation and functional differentiation were investigated in the clonal insulin-producing rat cell line RINm5F. Variations of the extracellular concentrations of Ca2+ and Mg2+ had only minor effects on the cellular contents of these elements. Even when the medium was depleted of extracellular Ca2+ by the addition of EGTA, the Ca/Mg ratio corresponded to approximately 0.1. In a Mg2+-deficient medium a lowering of the extracellular calcium to 0.13 mM reduced the amount of insulin in the media but increased that in the cells. Irrespective of the concentration of Mg2+, depletion of Ca2+ was associated with low media levels of insulin and a reduced proliferation rate. In addition, the cells became smaller in size and showed an increased ability to exclude trypan blue. a rise of the magnesium concentration to 6.30 mM was accompanied by a decrease of the cellular content of insulin despite reduced amounts of hormone in the medium. This finding might be due to suppression of insulin biosynthesis, suggesting that the RINm5F cells have a markedly different metabolism and/or sensitivity to extracellular magnesium than non-tumorigenic beta-cells.
authors.forename: V
authors.initials: V
authors.lastname: Hoftiezer
authors.forename: P O
authors.initials: PO
authors.lastname: Berggren
authors.forename: B
authors.initials: B
authors.lastname: Hellman
mesh_terms: D007516:Adenoma, Islet Cell / Q000473:pathology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis*; D007340:Insulinoma / Q000378:metabolism / Q000473:pathology*; D008274:Magnesium / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D051381:Rats
chemical_list: D007328:Insulin; D004533:Egtazic Acid; D008274:Magnesium; D002118:Calcium