Stimulation of Na+/H+ antiport activity by epidermal growth factor and insulin occurs without activation of protein kinase C.
Addition of phorbol 12, 13 dibutyrate (PBt2) or a combination of epidermal growth factor (EGF) and insulin to quiescent cultures of Swiss 3T3 cells increased intracellular pH in a Na+-dependent fashion. In contrast to PBt2, EGF plus insulin failed to stimulate protein kinase C and elicited the ionic response in cells lacking this enzyme. We suggest that the stimulation of the Na+/H+ antiport in Swiss 3T3 cells is mediated by at least two separate pathways, only one of which is dependent upon the activation of protein kinase C.
Vara
F
F
Rozengurt
E
E
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D004789:Enzyme Activation; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D010042:Ouabain / Q000494:pharmacology; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D011493:Protein Kinase C; D011494:Protein Kinases / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers
D002352:Carrier Proteins; D007328:Insulin; D010703:Phorbol Esters; D017923:Sodium-Hydrogen Exchangers; D015240:Phorbol 12,13-Dibutyrate; D000001:Calcimycin; D010042:Ouabain; D004815:Epidermal Growth Factor; D011494:Protein Kinases; D011493:Protein Kinase C; D012413:Rubidium; D011188:Potassium; D002118:Calcium