title: Role of the medial preoptic area in sexual behaviour of the male rat: a study using repeated cycloheximide infusions.
abstract: The effect of cycloheximide (CHX), an inhibitor of proteosynthesis, on sexual behaviour was studied in adult male rats in which it was infused into the medial preoptic area of the hypothalamus (MPOA). Sexual interaction took place under control and modification of the precopulatory behaviour of the female. Among the various bilaterally infused amounts of CHX--20 micrograms in 1 microliter, 40 micrograms in 1 microliter, 80 micrograms in 2 microliters--the effect of the largest dose corresponded to a hypothetical state of copulatory readiness of the males; 2 h after administration the males were not capable of initiating copulatory behaviour with a passively receptive female, but did so successfully with a highly soliciting female. The dependence of copulatory readiness of the males on the precopulatory behaviour of the females was confirmed repeatedly after this dose of CHX. At the same time, the precopulatory activity of the males towards a passively receptive female was unimpaired. The effect of CHX was reversible; 48 h after infusion the males displayed high copulatory readiness. Only half the males (n = 7) given bilateral infusions of 80 micrograms CHX fulfilled criterion of copulatory performance. Histological control demonstrated that the MPOA was affected bilaterally by CHX infusion in 10 males out of 14. The results are discussed from the aspect of participation of the MPOA in the regulation of male sexual behaviour. The method allows changes in sexual behaviour to be studied in the same individual in a chronic experiment.
authors.affiliation: Psychiatric Research Institute, Prague.
authors.forename: Z
authors.initials: Z
authors.lastname: Hlinák
authors.forename: M
authors.initials: M
authors.lastname: Spinka
authors.forename: J
authors.initials: J
authors.lastname: Madlafousek
authors.forename: F
authors.initials: F
authors.lastname: Semerád
mesh_terms: D000818:Animals; D002408:Catheters, Indwelling; D003513:Cycloheximide / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D008297:Male; D011301:Preoptic Area / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012726:Sexual Behavior, Animal / Q000187:drug effects*
chemical_list: D003513:Cycloheximide