title: Profiles of lymphokine activities and helper function for IgE in human T cell clones.
abstract: A large panel of phytohemagglutinin (PHA)-induced T cell clones (690 in total), established from four different human lymphoid tissues (peripheral blood, tonsils, lymph nodes and spleens) by a high-efficiency cloning technique, was characterized according to their pattern of lymphokine production. The majority of both CD4+ and CD8+ clones from all lymphoid tissues produced interleukin (IL) 2 and/or interferon (IFN)-gamma in response to 24-h stimulation with PHA. In contrast, higher proportions of IL 4-producing clones were found among CD4+ clones from tonsils and spleens than from peripheral blood and lymph nodes, whereas only a minority of CD8+ clones from all lymphoid tissues were found to produce IL 4. It was not possible to divide the CD4+ (helper/inducer) clones on the basis of their pattern of lymphokine activity into two clear-cut groups analogous to Th1 and Th2 helper clones described in mice. Although 21 out of 503 (4%) CD4+ T cell clones produced IL 4, but not IFN-gamma or IL 2, and 208 (41%) produced IL 2 and/or IFN-gamma, but not IL 4, a total number of 185 (37%) CD4+ clones showed the ability to produce IL 4 plus IL 2 and/or IFN-gamma. All types of CD4+ T cells (as classified according to their pattern of lymphokine activity) provided help for IgG production in allogeneic B cells. In contrast, helper function for IgE was detectable only among the IL 4-producing clones.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Allergology, University of Florence, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Maggi
authors.forename: G
authors.initials: G
authors.lastname: Del Prete
authors.forename: D
authors.initials: D
authors.lastname: Macchia
authors.forename: P
authors.initials: P
authors.lastname: Parronchi
authors.forename: A
authors.initials: A
authors.lastname: Tiri
authors.forename: I
authors.initials: I
authors.lastname: Chrétien
authors.forename: M
authors.initials: M
authors.lastname: Ricci
authors.forename: S
authors.initials: S
authors.lastname: Romagnani
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002999:Clone Cells; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4; D007378:Interleukins / Q000096:biosynthesis; D008211:Lymphocyte Cooperation; D008222:Lymphokines / Q000502:physiology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification / Q000276:immunology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D007074:Immunoglobulin G; D007376:Interleukin-2; D007378:Interleukins; D008222:Lymphokines; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma