[Isolation and purification of restriction endonuclease PmiI from Proteus mirabilis 1667].
A new restriction endonuclease Pmi I was detected in Proteus mirabilis 1667. The enzyme hydrolyzes DNA of the phage lambda into 10 electrophoretically separating fragments with molecular weights of 1.3-7.9 mD. With the use of two-stage chromatography on blue sepharose and phosphocellulose it is possible to obtain restriction endonuclease Pmi I free of the admixtures of ballast proteins, nonspecific nucleases and phosphatases.
Bakh
N L
NL
Tsvetkova
N V
NV
Semina
I E
IE
Tarasov
A P
AP
Mileĭkovskaia
M M
MM
D010582:Bacteriophage lambda; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; D011513:Proteus mirabilis / Q000201:enzymology*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C046158:endodeoxyribonuclease PmiI; D015252:Deoxyribonucleases, Type II Site-Specific
Vydelenie i ochistka restriktsionnoĭ éndonukleasy PmiI iz shtamma Proteus mirabilis 1667.