Activation of muscle phosphofructokinase by alpha-glucose 1,6-bisphosphate and fructose 2,6-bisphosphate is differently affected by other allosteric effectors and by pH.
Citrate, ATP and AMP affect similarly the activation of muscle phosphofructokinase by alpha-glucose 1,6-bisphosphate and fructose 1,6-bisphosphate, but they affect differently its activation by fructose 2,6-bisphosphate. Activation by alpha-glucose 1,6-bisphosphate and fructose 2,6-bisphosphate is also differently affected by pH. This suggest that both alpha-glucose 1,6-bisphosphate and fructose 1,6-bisphosphate induce the same conformational change on muscle phosphofructokinase, distinct from that produced by fructose 2,6-bisphosphate.
Andrés
V
V
Unitat de Bioquimica, Facultat de Medicina, Universitat de Barcelona, Spain.
Carreras
J
J
Cussó
R
R
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000494:Allosteric Regulation / Q000187:drug effects; D000818:Animals; D002951:Citrates / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005635:Fructosediphosphates / Q000494:pharmacology*; D005958:Glucosephosphates / Q000494:pharmacology*; D006598:Hexosediphosphates / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009132:Muscles / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D051381:Rats
D002951:Citrates; D005635:Fructosediphosphates; D005958:Glucosephosphates; D006598:Hexosediphosphates; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1