Antibodies against synthetic peptides react with the second Epstein-Barr virus-associated nuclear antigen.
Five peptides were synthesized on the basis of amino acid sequences predicted from the transformation-associated BamHI WYH region of the genome of the Epstein-Barr virus (EBV). Antisera to two peptides deduced from a 1.6-kb open reading frame in the BamHI H fragment identified an 87 000-dalton nuclear polypeptide that was present in EBV-carrying cell lines that expressed the second EBV-determined nuclear antigen (EBNA-2). This polypeptide was not detected in cell lines that carried EBV variants with a deleted BamHI WYH region or in EBV-negative cell lines. Three peptides deduced from the 1.6-kb open reading frame reacted with human EBNA-positive sera, but not with EBNA-negative sera. Following affinity purification with the peptides, two of the corresponding human antibodies also reacted with the 87 000-dalton polypeptide.
Dillner
J
J
Kallin
B
B
Klein
G
G
Jörnvall
H
H
Alexander
H
H
Lerner
R
R
D000595:Amino Acid Sequence; D000906:Antibodies; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004797:Enzyme-Linked Immunosorbent Assay; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000138:chemical synthesis; D013329:Structure-Activity Relationship; D014763:Viral Matrix Proteins
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D010455:Peptides; D014763:Viral Matrix Proteins; D004262:DNA Restriction Enzymes