title: Cytochrome b, the var 1 protein, and subunits I and III of cytochrome c oxidase are synthesized without transient presequences in Saccharomyces cerevisiae.
abstract: The N-termini of four mitochondrial translation products, the var 1 protein, cytochrome b, and subunits I and III of cytochrome c oxidase have been characterized in Saccharomyces cerevisiae and compared with the known DNA sequences of the respective structural genes. The four mature proteins correspond to the predicted primary translation products and retain the formylated methionine residue. Thus, subunit II of cytochrome c oxidase studied previously [Pratje et al. (1983) EMBO J.2, 1049-1054] is so far the only mitochondrial translation product carrying a N-terminal-extended transient presequence in S. cerevisiae.
authors.forename: G
authors.initials: G
authors.lastname: Mannhaupt
authors.forename: K
authors.initials: K
authors.lastname: Beyreuther
authors.forename: G
authors.initials: G
authors.lastname: Michaelis
mesh_terms: D000595:Amino Acid Sequence; D003573:Cytochrome b Group / Q000096:biosynthesis* / Q000235:genetics; D004271:DNA, Fungal / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000096:biosynthesis* / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D005796:Genes; D008565:Membrane Proteins; D008928:Mitochondria / Q000201:enzymology; D024101:Mitochondrial Proteins; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D003573:Cytochrome b Group; D004271:DNA, Fungal; D005656:Fungal Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D011498:Protein Precursors; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C026383:VAR1 protein, S cerevisiae; D003576:Electron Transport Complex IV