Hemadsorption focus assay for growth of influenza and parainfluenza viruses in human dermal fibroblasts.
An assay for virus-induced hemadsorption foci in cultures of human dermal fibroblasts is described. The assay allows determination of activity of species-specific as well as nonspecific antiviral agents or biological factors, such as interferons. The assay may be used for abortive (e.g., influenza virus) and productive (e.g., parainfluenza virus) infections of human fibroblasts.
Bell
D M
DM
Roberts
N J
NJ
Madore
H P
HP
Hall
C B
CB
Simons
R L
RL
D005347:Fibroblasts / Q000382:microbiology*; D006382:Hemadsorption; D006389:Hemagglutinins, Viral / Q000032:analysis; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D009975:Orthomyxoviridae / Q000254:growth & development*; D018108:Respirovirus / Q000254:growth & development*; D012867:Skin / Q000382:microbiology; D014776:Virus Cultivation
D006389:Hemagglutinins, Viral; D007370:Interferon Type I