Production of superoxide anion by bovine pulmonary macrophages challenged with soluble and particulate stimuli.
The effects of opsonized zymosan, phorbal myristate acetate, and live Pasteurella haemolytica on superoxide anion production by bovine pulmonary macrophages were determined. The anion responses were dose-dependent for all stimuli, except for unopsonized P haemolytica. The effect of viable P haemolytica on macrophage viability was related to bacterial dosage and the presence of opsonizing antibody. Superoxide responses varied directly with the dose of opsonized live P haemolytica, but indirectly with macrophage viability.
Dyer
R M
RM
Benson
C E
CE
Boy
M G
MG
D000818:Animals; D002417:Cattle; D003124:Colorimetry; D007106:Immune Sera / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010325:Pasteurella / Q000502:physiology*; D010704:Phorbols / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000166:cytology; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology*
D007106:Immune Sera; D010704:Phorbols; D013481:Superoxides; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate