title: Induction of collagenase secretion in human fibroblast cultures by growth promoting factors.
abstract: In human foreskin fibroblast cultures, two proteins with Mr 60,000 and 55,000 were found to be induced about 3.5-fold by epidermal growth factor (EGF), platelet-derived growth factor, and beta-transforming growth factor. The induced proteins were identified as procollagenases by immunoprecipitation of induced medium with antibodies to purified human fibroblast collagenase. Collagenase enzyme activity in the medium from EGF-treated cultures was also induced at least 3-fold compared to control cultures. Induction of collagenase was dependent upon de novo protein and RNA synthesis and was observed in the medium 10 h after addition of EGF. Although these growth-promoting factors interact with separate membrane receptors, each induced the secretion of a common protein, suggesting that collagenase may be important in some aspect of mitogenesis, cell mobilization, and migration.
authors.forename: C C
authors.initials: CC
authors.lastname: Chua
authors.forename: D E
authors.initials: DE
authors.lastname: Geiman
authors.forename: G H
authors.initials: GH
authors.lastname: Keller
authors.forename: R L
authors.initials: RL
authors.lastname: Ladda
mesh_terms: D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D017364:Collagenases; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D004792:Enzyme Precursors / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000201:enzymology*; D006801:Humans; D003012:Microbial Collagenase / Q000096:biosynthesis / Q000378:metabolism*; D008970:Molecular Weight; D010455:Peptides / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D013997:Time Factors; D015291:Transforming Growth Factors
chemical_list: D004792:Enzyme Precursors; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D003094:Collagen; D017364:Collagenases; C022440:procollagenase; D003012:Microbial Collagenase