Infection of HTLV-III/LAV in HTLV-I-carrying cells MT-2 and MT-4 and application in a plaque assay.
The human T-cell lines MT-2 and MT-4 carry the human T-cell leukemia virus type I (HTLV-I). When MT-2 and MT-4 were infected with HTLV-III, the probable etiologic agent of the acquired immune deficiency syndrome (AIDS), rapid cytopathogenic effects and cytotoxicity were observed that made it possible to titrate the biologically active virus in a plaque-forming assay. The cytopathogenic effects were preceded by the rapid induction and increase of HTLV-III antigens as revealed by immunofluorescence and immunoprecipitation. Activities of HTLV-III were neutralized by the human antibodies against the virus when immunofluorescence and plaque assays were used. Essentially the same results were obtained with the lymphadenopathy-associated virus (LAV1).
Harada
S
S
Koyanagi
Y
Y
Yamamoto
N
N
D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D011232:Chemical Precipitation; D003588:Cytopathogenic Effect, Viral; D017977:Deltaretrovirus / Q000254:growth & development* / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007120:Immunochemistry; D007938:Leukemia; D013601:T-Lymphocytes; D010948:Viral Plaque Assay; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
D000956:Antigens, Viral