Coliphage lambda to terminator lowers the stability of messenger RNA in Escherichia coli hosts.
The effects of the transcription terminators to and tfd on the overall high-level expression of a human interferon-beta gene (IFN-beta) in Escherichia coli hosts were compared. Deletion mapping shows that mRNA lability is caused by sequences at or near the lambda terminator to stem-loop structure. Extensive RNA secondary structure in this region indicates a potential RNase III cleavage/binding site. In RNase III- E. coli hosts, IFN-beta synthesis is indeed considerably enhanced. The bacteriophage tfd terminator does not confer this mRNA labilization phenomenon. In all cases, RNA level and stability correlate with the level of IFN-beta synthesized in the cell. In the system described, ongoing translation stabilizes mRNA only moderately.
Gross
G
G
Gesellschaft für Biotechnologische Forschung, Abteilung Genetik, Braunschweig, F.R.G.
Hollatz
I
I
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005822:Genetic Vectors; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins; D014158:Transcription, Genetic
D007370:Interferon Type I; D012333:RNA, Messenger; D011994:Recombinant Proteins