The platelet activation induced by wheat germ agglutinin.
In human platelets, wheat germ agglutinin (WGA) induced serotonin release without cell agglutination. WGA induced the phosphorylation of both 40-kDa and 20-kDa proteins in a parallel manner, and at least, the phosphorylation of 40-kDa protein was preceded by transient formation of endogenous diacylglycerol (DG) accompanied by a decrease in phosphatidylinositol (PI). Both phosphorylation of these two proteins and serotonin release were inhibited by prior treatment of platelets with dibutyryl cyclic AMP, W-7, or TMB-8. These results suggest that both phosphatidylinositol turnover and Ca2+ mobilization play an essential role in WGA-induced platelet activation.
Higashihara
M
M
Takahata
K
K
Ohashi
T
T
Kariya
T
T
Kume
S
S
Oka
H
H
D000634:Aminoquinolines; D001792:Blood Platelets / Q000187:drug effects*; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000097:blood; D002751:Chlortetracycline; D004075:Diglycerides / Q000097:blood; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D010710:Phosphates / Q000097:blood; D010716:Phosphatidylinositols / Q000097:blood; D010766:Phosphorylation; D012701:Serotonin / Q000097:blood; D013449:Sulfonamides / Q000494:pharmacology; D014909:Wheat Germ Agglutinins
D000634:Aminoquinolines; D004075:Diglycerides; D037102:Lectins; D010710:Phosphates; D010716:Phosphatidylinositols; D013449:Sulfonamides; D014909:Wheat Germ Agglutinins; D012701:Serotonin; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D003994:Bucladesine; C017967:W 7; C035739:Quin2; D002118:Calcium; D002751:Chlortetracycline