title: Changes in vasopressin-converting aminopeptidase activity in the rat pineal gland during summer: relationship to vasopressin contents.
abstract: Vasopressin (VP)-converting aminopeptidase (VP-AP) activity and VP contents were measured in single rat pineal glands during the summer of two successive years. The peptidase activity decreased significantly in August. The lowest activity (+/- SEM) of 0.18 +/- 0.02 pmol.hour-1 was recorded on August 14, compared to the basal activity of 0.25 +/- 0.01 pmol.hour-1 in July and September of 1986. The change with similar percentage occurred in the same period of 1987. The specific activity of the enzyme in the crude homogenate, 15,000 g pellet and supernatant fraction of rat pineal glands, exhibited the same pattern of variations. The decrease in peptidase activity coincided with the previously reported dramatic rise in pineal VP content in early August which was confirmed in this series of experiments. Another peptidase, the so-called gamma-endorphin generating endopeptidase (gamma-EGE) activity, and beta-endorphin-related peptides in the pineal gland did not change in this period. The results show that the variations of pineal VP contents and VP-AP activity during summer are not general for other peptides and peptidases. The coincidence of opposite changes in VP content and VP-AP activity of the pineal gland may indicate a role of the peptidase activity to regulate the VP content.
authors.affiliation: Rudolf Magnus Institute for Pharmacology, University of Utrecht, The Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.forename: J P
authors.initials: JP
authors.lastname: Burbach
mesh_terms: D000064:Acclimatization; D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D008297:Male; D010870:Pineal Gland / Q000201:enzymology / Q000502:physiology*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012621:Seasons; D014667:Vasopressins / Q000378:metabolism*; D001615:beta-Endorphin / Q000032:analysis
chemical_list: D014667:Vasopressins; D001615:beta-Endorphin; D000626:Aminopeptidases; C052354:vasopressin-converting aminopeptidase