High level human interleukin 1 production by a hepatoma cell line.
The human hepatic adenocarcinoma cell line, SK-hep-1, was found to constitutively produce Interleukin 1. Addition of the ionophore A23187 and lipopolysaccharide resulted in a 30-fold enhancement in the release of biological activity. Serum supplementation did not affect the level of production. Interleukin 1 from these cells had a molecular weight of 10-20,000 daltons on gel exclusion chromatography. Polyadenylated RNA, when fractionated on sucrose density gradients and injected into Xenopus laevis oocytes, produced high levels of biological activity in the 14-16s region. An oligonucleotide probe, complementary to the coding sequence of the Interleukin 1 cDNA isolated from human monocytes, hybridized specifically to this part of the gradient. These results demonstrate that SK-hep-1 cells are a valuable source of material for studying the polypeptide and messenger RNA of Interleukin 1.
Doyle
M V
MV
Brindley
L
L
Kawasaki
E
E
Larrick
J
J
D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D000001:Calcimycin / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008113:Liver Neoplasms / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009865:Oocytes / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D014982:Xenopus laevis
D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D000001:Calcimycin