A simple purification method for a glucosyltransferase complex from Streptococcus mutans OMZ 176 with a high yield.
1. Glucosyltransferase was purified from Streptococcus mutans OMZ 176 by ammonium sulfate fractionation and gel filtration. 2. The overall purification was about 15,000-fold with a yield of 53%. 3. The purified enzyme appeared to be a multienzyme complex consisting of three different components with molecular weights of 158,000, 153,000 and 149,000, respectively.
Furutani
M
M
Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Japan.
Iwaki
M
M
Yagi
T
T
Iida
M
M
Horiike
K
K
Nozaki
M
M
D000645:Ammonium Sulfate; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel; D005964:Glucosyltransferases / Q000302:isolation & purification*; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D013291:Streptococcus / Q000201:enzymology*
D011135:Polysaccharides, Bacterial; D005964:Glucosyltransferases; D000645:Ammonium Sulfate