title: Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter.
abstract: In vitro expression of cDNA encoding bovine opsin is accomplished using the baculovirus expression vector system. Full-length opsin was synthesized which was recognized by poly- and monoclonal antisera raised against bovine rhodopsin. Upon infection with a recombinant virus, 1 x 10(6) insect cells produced up to 3 micrograms opsin. Incubation of the in vitro synthesized opsin with 11-cis retinal produced a hydroxylamine-stable, photosensitive pigment.
authors.affiliation: Department of Biochemistry, University of Nijmegen, The Netherlands.
authors.forename: J J
authors.initials: JJ
authors.lastname: Janssen
authors.forename: W J
authors.initials: WJ
authors.lastname: van de Ven
authors.forename: W A
authors.initials: WA
authors.lastname: van Groningen-Luyben
authors.forename: J
authors.initials: J
authors.lastname: Roosien
authors.forename: J M
authors.initials: JM
authors.lastname: Vlak
authors.forename: W J
authors.initials: WJ
authors.lastname: de Grip
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors; D007304:Insect Viruses / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017299:Rod Opsins
chemical_list: D005136:Eye Proteins; D011994:Recombinant Proteins; D017299:Rod Opsins; D004247:DNA