title: The human apolipoprotein A-II gene: complete nucleic acid sequence and genomic organization.
abstract: The gene for human apolipoprotein (apo) A-II has been isolated from a human genomic DNA library. The cloned fragment was approximately 14 kilobase-pair (kb) long, and extended about 9.0 kb upstream as well as 3.5 kb downstream from the apoA-II gene, which was contained within a 3.1 kb HindIII fragment of human DNA. The complete nucleic acid sequence of the apoA-II gene has been determined, establishing that the apoA-II gene is interrupted by three intervening sequences of 182, 293, and 395 bp. The second intron is of particular interest, because it contains a 33 bp sequence of alternating G and T residues very close to the 3' splice site which has the potential to form a left handed Z-helix structure in vivo. A restriction fragment length polymorphism 3' from the apoA-II gene has been detected which may serve as a marker for the long arm of chromosome 1 in linkage analyses.
authors.forename: K J
authors.initials: KJ
authors.lastname: Lackner
authors.forename: S W
authors.initials: SW
authors.lastname: Law
authors.forename: H B
authors.initials: HB
authors.lastname: Brewer
mesh_terms: D016633:Apolipoprotein A-II; D001054:Apolipoproteins A / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D005796:Genes; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids
chemical_list: D016633:Apolipoprotein A-II; D001054:Apolipoproteins A; D004279:DNA, Viral; D008075:Lipoproteins, HDL; D004247:DNA; D004262:DNA Restriction Enzymes