Protection from genital herpes simplex virus type 2 infection by vaccination with cloned type 1 glycoprotein D.
Guinea pigs were vaccinated with truncated herpes simplex virus type-1 (HSV-1) glycoprotein D produced in the genetically engineered mammalian cell line gD10.2. Vaccinated animals formed antibodies that neutralized both HSV-1 and herpes simplex virus type 2 (HSV-2) in an in vitro neutralization assay. Vaccinated animals were challenged with HSV-2 by intravaginal infection. Animals that received the immunogen in Freund's complete adjuvant were completely protected from the clinical manifestations of genital HSV-2 infection. Animals that received the immunogen incorporated in alum adjuvants were partly protected from clinical disease; the infections that did develop were significantly less severe than those that occurred in control animals injected with adjuvant alone. The results demonstrate that immunization with a purified viral protein can provide significant protection against primary genital infection by HSV-2 in guinea pigs.
Berman
P W
PW
Gregory
T
T
Crase
D
D
Lasky
L A
LA
D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D000536:Aluminum Hydroxide; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D003001:Cloning, Molecular; D005260:Female; D005620:Freund's Adjuvant; D006168:Guinea Pigs; D006558:Herpes Genitalis / Q000517:prevention & control*; D008297:Male; D009500:Neutralization Tests; D010710:Phosphates; D018139:Simplexvirus / Q000276:immunology*; D014611:Vaccination; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D000914:Antibodies, Viral; D010710:Phosphates; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C040570:glycoprotein D-herpes simplex virus type 2; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant; C012714:aluminum phosphate