Discovery of novel brain lipoxygenase products formed from docosahexaenoic acid (22:6w3).
It is known that alcohol exposure leads to a decline in brain docosahexaenoate (22:6w3). We hypothesized that alcohol could stimulate the metabolism of this polyunsaturated fatty acid to bioactive products. Several oxidized products of 22:6w3 were indeed observed when rat brain homogenate was incubated with 14C22:6w3 in vitro. A similar group of metabolites was formed in vivo from 14C-22:6w3 injected into the lateral ventricle. These metabolites were characterized by thermospray- and GC/MS as well as by the synthesis of standards using purified enzymes. Platelet lipoxygenase also proved useful in identifying one of the brain metabolites and served as a source of enzyme for preparative studies. Their physiological effects on smooth muscle tone and platelet aggregation will be presented.
Shingu
T
T
Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892.
Karanian
J W
JW
Kim
H Y
HY
Yergey
J A
JA
Salem
N
N
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D008084:Lipoxygenase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004281:Docosahexaenoic Acids; D008084:Lipoxygenase