The influence of cell population density and serum on the (Na+K)-ATPase of 3T3 cells.
The (Na+K)-ATPase of 3T3 cells has been measured as a function of culture density and the type of serum in which the cells were grown. When 3T3 cells were grown in medium containing 10% newborn calf serum their (Na+K)-ATPase activity increased as the culture density increased and the cells became quiescent at densities of about 17.5 X 10(4) cells/cm2. When these cells are subcultured into medium containing 10% foetal bovine serum the activity of the enzyme decreases as the culture density increases and the cells attain final densities of about 5 X 10(4) cells/cm2.
McCaldin
B
B
Tenang
E M
EM
D000818:Animals; D001769:Blood; D002417:Cattle; D002452:Cell Count; D002455:Cell Division; D002460:Cell Line; D002477:Cells / Q000201:enzymology*; D002478:Cells, Cultured; D003470:Culture Media; D051379:Mice; D008815:Mice, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D003470:Culture Media; D000254:Sodium-Potassium-Exchanging ATPase