Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.
A series of human carcinoma cell lines was examined for human papillomavirus (HPV) DNA sequences with the use of HPV-6, HPV-11, HPV-16, and HPV-18 DNA probes. Six of eight cell lines derived from human cervical carcinomas were shown to contain integrated HPV DNA sequences. In five of these six lines, HPV-specific polyadenylated RNA species could also be identified. The expression of HPV sequences was detected in three lines with a HPV-18 DNA probe and in two lines with a HPV-16 DNA probe. Of the two lines which contained HPV-16 specific RNA, one contained HPV DNA sequences which hybridized only to an HPV-16 probe, and the other contained HPV DNA sequences which hybridized to both HPV-16 and HPV-18 DNA probes. Six cell lines established from human squamous-cell carcinomas of the bladder, pharynx, lung, esophagus, and vulva were negative for HPV-6, HPV-11, HPV-16, and HPV-18 DNA sequences under stringent hybridization conditions.
Yee
C
C
Krishnan-Hewlett
I
I
Baker
C C
CC
Schlegel
R
R
Howley
P M
PM
D001483:Base Sequence; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D004279:DNA, Viral; D012367:RNA, Viral