Glycoprotein modifications of sarcoma L-1 tumor cells by tunicamycin, swainsonine, bromoconduritol or 1-desoxynojirimycin treatment inhibits their metastatic lung colonization in Balb/c-mice.
Synthesis and expression of cell surface carbohydrates appear to be involved in recognition events associated with tumor invasion and metastasis. Thus, the potential of murine sarcoma L-1 cells to form experimental lung metastases after i.v. injection was assessed after inhibiting tumor cell protein glycosylation with tunicamycin, swainsonine, bromoconduritol, or 1-desoxynojirimycin. Incubation of sarcoma L-1 cells with 0.5 microgram (or above) of these substances/ml medium for 20-24 h significantly inhibited lung colonization. Cytotoxic side effects or additional organ manifestations could not be found. Gas liquid chromatographic examinations of carbohydrates from treated L-1 cells indicated that sugar synthesis was evidently inhibited. These results suggest that specific glycan structures on tumor cells are required for expression of the metastatic phenotype.
Pulverer
G
G
Institute of Hygiene, University of Cologne, Federal Republic of Germany.
Beuth
J
J
Ko
H L
HL
Yassin
A
A
Ohshima
Y
Y
Roszkowski
K
K
Uhlenbruck
G
G
D017485:1-Deoxynojirimycin; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D053138:Cyclohexenes; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000032:analysis* / Q000502:physiology*; D007294:Inositol / Q000031:analogs & derivatives* / Q000494:pharmacology; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012513:Sarcoma, Experimental / Q000473:pathology*; D017026:Swainsonine; D014415:Tunicamycin / Q000494:pharmacology*
D000470:Alkaloids; D002241:Carbohydrates; D053138:Cyclohexenes; D006023:Glycoproteins; D014415:Tunicamycin; D017485:1-Deoxynojirimycin; C036388:6-bromoconduritol; D007294:Inositol; D005944:Glucosamine; D017026:Swainsonine