Immunological study of the rat hypothalamic ventromedial nucleus.
Specific anti-sera were prepared by injecting the homogenates of rat ventromedial nucleus (VMH) and caudate nucleus (Cd) into the rabbit. Anti-VMH serum, after absorption of common components in rat normal serum and anti-Cd serum, reacted specifically to the rat VMH with only one precipitation line. Anti-VMH serum was successfully applied to 80 VMH neurons by electrophoresis through 5 barreled micropipettes. Fresh anti-VMH serum caused an irreversible response (increase and subsequent sudden cessation of firing) in 8 of the 25 VMH neurons tested. Anti-VMH serum reversibly inhibited 32 of 80 VMH neurons and 13 of these were also tested with glucose. Discharge rates of 12 of the 13 neurons increased by glucose. Most of these neurons were not affected by anti-Cd serum or normal rabbit serum. Results of these immunological and electrophysiological studies suggest the existence of specific membrane receptor binding sites on the glucoreceptor neurons in the VMH. These sites afford one route for producing the excitatory effect that glucose has on VMH neurons.
Oomura
Y
Y
Ono
T
T
Sugimori
M
M
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D002421:Caudate Nucleus / Q000276:immunology; D002540:Cerebral Cortex / Q000276:immunology; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008297:Male; D009474:Neurons / Q000145:classification / Q000276:immunology / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D009435:Synaptic Transmission; D014697:Ventromedial Hypothalamic Nucleus / Q000276:immunology* / Q000502:physiology
D000906:Antibodies