title: Modulation of oligosaccharide processing in an exocrine secretory glycoprotein of rat parotid cells by beta-adrenoreceptor activation.
abstract: Such stimulation of rat parotid acinar cells in vitro modulated the rate of processing of N-linked oligosaccharides in a high-molecular weight (220 kdalton) secretory glycoprotein. Conversion of polymannose-type oligosaccharides to complex-type oligosaccharides was evaluated by sensitivity to endoglucosaminidase H and alpha-mannosidase, and with a specific inhibitor of glucosidases I/II. Oligosaccharide maturation in the 220 kdalton glycoprotein required one-third to half less time in cells exposed to the beta-adrenergic agonist isoproterenol than in controls.
authors.affiliation: Clinical Investigations and Patient Care Branch, National Institute of Dental Research, Bethesda, MD 20892.
authors.forename: E E
authors.initials: EE
authors.lastname: Kousvelari
authors.forename: D K
authors.initials: DK
authors.lastname: Banerjee
authors.forename: S R
authors.initials: SR
authors.lastname: Grant
authors.forename: B J
authors.initials: BJ
authors.lastname: Baum
mesh_terms: D017485:1-Deoxynojirimycin; D000818:Animals; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009844:Oligosaccharides / Q000378:metabolism*; D010306:Parotid Gland / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D017485:1-Deoxynojirimycin; D007545:Isoproterenol; D005944:Glucosamine