Characterization of the gamma-aminobutyric acid receptor system in human brain gliomas.
The properties of [3H]-gamma-aminobutyric acid [( 3H]GABA) binding were studied in biopsied specimens from normal human brain and from 18 cases of human brain gliomas, made up of 6 astrocytomas, 6 glioblastomas, 3 oligodendrogliomas, and 3 medulloblastomas. In fresh membranes obtained from normal gray and white matter one population of Na+-dependent GABA receptors was observed, while in the frozen Triton X-100-treated membranes two distinct populations of Na+-independent binding sites were detected. Specific GABA binding sites in brain gliomas were shown only in frozen Triton X-100-treated membranes. As in normal tissue, these receptors are Na+-independent and bind [3H]GABA with two distinct affinity components. The biochemical profiles of [3H]GABA binding to membranes obtained from different tumors of glial origin are quite similar and cannot be related to the degree of malignancy of the neoplasia.
Frattola
L
L
Ferrarese
C
C
Canal
N
N
Gaini
S M
SM
Galluso
R
R
Piolti
R
R
Trabucchi
M
M
D001932:Brain Neoplasms / Q000032:analysis*; D005910:Glioma / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D011963:Receptors, GABA-A / Q000032:analysis*; D012964:Sodium / Q000494:pharmacology; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D011963:Receptors, GABA-A; D014316:Tritium; D005680:gamma-Aminobutyric Acid; D012964:Sodium