The expression of B cell surface receptors. I. The ontogeny and distribution of the murine B cell IgE Fc receptor.
The ontogenic appearance and lymphoid tissue distribution of the murine B cell IgE FcR (Fc epsilon R) was examined. Flow cytometry was utilized to study the expression of the Fc epsilon R on splenic B cells from mice of increasing age, as well as B cells from various lymphoid organs. A large panel of B cell tumors was also screened for the presence of the Fc epsilon R. The results demonstrate that the Fc epsilon R appears very late in B cell development, and is preceded in appearance even by IgD. In adult animals, the Fc epsilon R was found to be expressed on virtually all mature IgM, IgD bearing B cells, whether taken from the spleen, lymph nodes, or Peyer's patches. Further examination showed that B cells which had switched to express an isotype other than IgD, appeared to no longer display the Fc epsilon R. When surveying a variety of B cell tumors, the Fc epsilon R was found to be present on WEHI 279, an IgM, IgD-bearing lymphoma. The receptor was not found on pre-B cell, immature B cell, switched B cell, or secreting B cell tumors. Taken together, these results indicate that the B cell Fc epsilon R is expressed predominantly on mature, virgin B cells, and is lost after activation and switching.
Waldschmidt
T J
TJ
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.
Conrad
D H
DH
Lynch
R G
RG
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D005260:Female; D005434:Flow Cytometry; D007073:Immunoglobulin E / Q000378:metabolism*; D008221:Lymphoid Tissue / Q000166:cytology; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E