title: Alterations in lactate dehydrogenase isoenzymes and phosphofructokinase activity in the hormonally induced G-XII cell line.
abstract: G-XII cell line was earlier developed in our laboratory from the granulosa lutein cells of goat ovaries, subjected to sustained stimulation by ovine luteinizing hormone. It is a non-clonogenic and non-tumorigenic cell line which secretes progesterone. In the present paper, we report some biochemical findings on the cell line studied between passages 10 and 15. The cells exhibited a shift in the lactate dehydrogenase isoenzyme pattern from the heart form to muscle form and a 300% increase in the phosphofructokinase activity compared to fresh granulosa cells.
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
authors.forename: A K
authors.initials: AK
authors.lastname: Malik
authors.forename: N
authors.initials: N
authors.lastname: Sharma
authors.forename: P
authors.initials: P
authors.lastname: Chandiok
authors.forename: T N
authors.initials: TN
authors.lastname: Chapekar
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D005812:Genes, Switch; D006107:Granulosa Cells / Q000187:drug effects / Q000201:enzymology*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D010732:Phosphofructokinase-1 / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D007986:Luteinizing Hormone; D007770:L-Lactate Dehydrogenase; D010732:Phosphofructokinase-1