Regulation of a cya-lac fusion by cyclic AMP in Salmonella typhimurium.
cya-lac and crp-lac operon fusions were isolated in Salmonella typhimurium by using the phage Mu d1(lac cts Apr). Both transduction and reversion analyses have indicated that lac expression is controlled by the appropriate promoter, e.g., either crpp or cyap. By using chromosomal mobilization techniques, we found that cya had a clockwise direction of transcription on the standard S. typhimurium map. The cya-lac fusions could be complemented by Escherichia coli F'133, which covers cya, with a resultant 17 to 38% decrease in cya expression. Cyclic AMP was found to be able to repress the expression of the cya-lac fusion ninefold when present at 25 mM. This repression was not seen in crp backgrounds, and hence is mediated by the cAMP receptor protein. Repression of cya was also found upon growth on carbon sources known to elicit high cyclic AMP levels.
Jovanovich
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SB
D000262:Adenylyl Cyclases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D000242:Cyclic AMP / Q000494:pharmacology*; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D006460:Hemolysin Proteins; D007763:Lac Operon; D010957:Plasmids; D012486:Salmonella typhimurium / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D003470:Culture Media; D006460:Hemolysin Proteins; D002244:Carbon; D000242:Cyclic AMP; D000262:Adenylyl Cyclases