In vitro recombination of bacteriophage T7 DNA: further characterization of the reaction using plasmid DNA.
We have used a plasmid which contains a cloned fragment of T7 DNA to study the properties of general recombination of phage T7 in vitro. It was shown that T7-infected cell extracts promote recombination by the exchange of double strands of DNA. While both products of these double-strand exchanges were detected, we were unable to show that they were formed during a single recombination event.
Lee
D
D
Vetter
D
D
Beatty
L
L
Sadowski
P
P
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic; D013604:T-Phages / Q000235:genetics*
D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes