High-resolution polyacrylamide gradient gel electrophoresis (PGGE) of isoenzymes from five Naegleria species.
High-resolution polyacrylamide gradient gel electrophoresis (PGGE) was used to separate isoenzymes of 12 Naegleria strains: one N. australiensis, two N. lovaniensis, one N. jadini, two N. gruberi isolated from environmental samples, and six N. fowleri strains isolated from patients with primary amoebic meningoencephalitis. Of the eight enzymes studied, seven showed zymograms with interspecific variation that identified all the species tested. Although the six N. fowleri strains were biochemically the most homogeneous, they showed intraspecific isoenzyme variation that allowed them to be grouped into four zymodemes. The PGGE technique, which separates isoenzymes by their molecular shape, is both sensitive and economical. It offers an addition or an attractive alternative to isoelectric focusing which has commonly been used to aid species identification of Naegleria by separating isoenzymes by their isoelectric point.
Moss
D M
DM
U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Georgia 30333.
Brandt
F H
FH
Mathews
H M
HM
Visvesvara
G S
GS
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D005969:Glutamate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis*; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D009253:Naegleria / Q000145:classification / Q000201:enzymology*; D010733:Phosphoglucomutase / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis
D007527:Isoenzymes; D008291:Malate Dehydrogenase; D013482:Superoxide Dismutase; D005969:Glutamate Dehydrogenase; D002265:Carboxylic Ester Hydrolases; C034216:propionyl esterase; D000135:Acid Phosphatase; D007931:Leucyl Aminopeptidase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase