title: Cloning of sporulation gene spoIVC in Bacillus subtilis.
abstract: Sporulation gene spoIVC of Bacillus subtilis was cloned by the prophage transformation method in temperate phage phi 105. The specialized transducing phage, phi 105 spoIVC-1, restored the sporulation of the asporogenous mutant of B. subtilis strain 1S47 (spoIVC133). Transformation experiments showed that the spoIVC gene resides on a 7.3 kb HindIII restriction fragment. Subsequent analysis of the 7.3 kb HindIII fragment with restriction endonuclease EcoRI showed that the spoIVC gene resides on a 3.6 kb EcoRI fragment within the 7.3 kb fragment. The 3.6 kb fragment was recloned into the unique EcoRI site of plasmid pUB110 and deletion derivatives having a deletion within the 3.6 kb insert were constructed. The plasmid carrying the entire spoIVC gene restored the sporulation of strain HU1214 (spoIVC133, recE4) at a frequency of 10(7) spores/ml, and reduced the sporulation of strain HU1018 (spo+, recE4) to 10(7) spores/ml.
authors.forename: M
authors.initials: M
authors.lastname: Fujita
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005798:Genes, Bacterial; D005838:Genotype; D010957:Plasmids; D013045:Species Specificity; D013171:Spores, Bacterial / Q000502:physiology; D014161:Transduction, Genetic
chemical_list: D004262:DNA Restriction Enzymes