Modulation of oligosaccharide processing in an exocrine secretory glycoprotein of rat parotid cells by beta-adrenoreceptor activation.
Such stimulation of rat parotid acinar cells in vitro modulated the rate of processing of N-linked oligosaccharides in a high-molecular weight (220 kdalton) secretory glycoprotein. Conversion of polymannose-type oligosaccharides to complex-type oligosaccharides was evaluated by sensitivity to endoglucosaminidase H and alpha-mannosidase, and with a specific inhibitor of glucosidases I/II. Oligosaccharide maturation in the 220 kdalton glycoprotein required one-third to half less time in cells exposed to the beta-adrenergic agonist isoproterenol than in controls.
Kousvelari
E E
EE
Clinical Investigations and Patient Care Branch, National Institute of Dental Research, Bethesda, MD 20892.
Banerjee
D K
DK
Grant
S R
SR
Baum
B J
BJ
D017485:1-Deoxynojirimycin; D000818:Animals; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009844:Oligosaccharides / Q000378:metabolism*; D010306:Parotid Gland / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D006023:Glycoproteins; D009844:Oligosaccharides; D017485:1-Deoxynojirimycin; D007545:Isoproterenol; D005944:Glucosamine