Hydrogen peroxide and hydroxyl free radical production by hematoporphyrin derivative, ascorbate and light.
Photofrin II, a commercial preparation of a hematoporphyrin derivative containing a high proportion of the active di-hematoporphyrin ether, was found to convert oxygen to hydrogen peroxide and hydroxyl free radicals in the presence of ascorbate and light. In addition, a light-dependent increase in the ascorbate free radical concentration was observed in hematoporphyrin derivative/ascorbate solutions with subsequent oxidation of ascorbate.
Buettner
G R
GR
Need
M J
MJ
D001205:Ascorbic Acid / Q000378:metabolism* / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000378:metabolism* / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000528:radiation effects; D066298:In Vitro Techniques; D008027:Light; D010100:Oxygen / Q000378:metabolism / Q000528:radiation effects
D005609:Free Radicals; D006415:Hematoporphyrins; D017324:Hematoporphyrin Derivative; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; D010100:Oxygen