Effects of an organosilicon compound on the tubular apparatus of rat kidney. A histological and enzyme histochemical report.
Male adult Wistar rats were treated for 8 weeks with an organosilicone [2,2-dimethyl-4-(chloromethyl)-1,3-dioxa-2-silacyclopentane] employing two different dosages (25 and 50 mg/kg body weight i.p. daily) and the changes in the tubular apparatus of the kidney were investigated employing histological and enzyme-histochemical methods. The effects, more pronounced at the higher dosage, were the following: The brush borders of the proximal tubules were stuck together and disintegrated; only few epithelial cells remained intact showing a decreased activity of nonspecific esterases and the increase of beta-hydroxybutyric acid dehydrogenase. The nuclei of most of the epithelial cells in the distal tubules were dislocated towards the enlarged lumen and the cytoplasma showed a decrease of nonspecific esterases and an increase of beta-hydroxybutyric acid dehydrogenase. The collagen fibers in the walls of the collecting tubules were dislocated and disintegrated with a discontinuous border of Mg2+-ATPase; the nuclei of the epithelial cells were pyknotic, the cytoplasma showed an increase of beta-hydroxybutyric acid dehydrogenase. The induced changes were partially reversible after a recovery period of 8 weeks.
Haider
S G
SG
Institute of Anatomy II, University of Düsseldorf, FRG.
Rolauffs
D
D
Goslar
H G
HG
Stuhl
O
O
Birkofer
L
L
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology*; D004950:Esterases / Q000378:metabolism; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects*; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000187:drug effects; D007686:Kidney Tubules, Distal / Q000166:cytology / Q000187:drug effects; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000187:drug effects; D008297:Male; D017646:Organosilicon Compounds; D051381:Rats; D011919:Rats, Inbred Strains; D012825:Silicon / Q000494:pharmacology*
D003517:Cyclopentanes; D017646:Organosilicon Compounds; C039270:2,2-dimethyl-4-(chloromethyl)-1,3-dioxa-2-silacyclopentane; D006884:Hydroxybutyrate Dehydrogenase; D004950:Esterases; D017301:Ca(2+) Mg(2+)-ATPase; D012825:Silicon