[Streptozocin-sensitized lymphocytes suppress the glucose-stimulated insulin secretion of the islands of Langerhans].
Isolated islets of Langerhans (from C57Bl/6J mice) were either incubated with culture medium only or medium substituted by 1 mM streptozocin (SZ) and cultivated with lymphocytes (Ly) for seven days. Ly were isolated from the same mouse strain sensitized against SZ by 5 x 40 mg SZ per kg and day intraperitoneally or 1 mg SZ subcutaneously in vivo. Glucose-stimulated insulin secretion rate was used as an indicator for the metabolic condition of islets. SZ-pretreated islets showed significantly decreased insulin secretion in the presence of Ly. The secretion capacity of untreated islets was not impaired.
Linn
T
T
Medizinische Klinik III, Universität Giessen.
Marzillier
J
J
Flesch
B
B
Federlin
K
K
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013311:Streptozocin / Q000494:pharmacology*
D003470:Culture Media; D007328:Insulin; D013311:Streptozocin; D005947:Glucose
Streptozocin-sensibilisierte Lymphozyten unterdrücken die glukosestimulierte Insulinsekretion Langerhansscher Inseln.