title: Platelets mediate neutrophil-dependent immune complex nephritis in the rat.
abstract: Neutrophils and platelets are frequently present in glomeruli in immune glomerulonephritis (GN). No role for the platelet in acute neutrophil-mediated renal injury has been defined. We investigated a neutrophil-mediated model of subendothelial immune complex GN in the rat. Rats were platelet-depleted (mean platelet less than 10,000/microliter) with goat anti-platelet IgG before induction of GN by the renal artery perfusion of concanavalin A followed by anti-concanavalin A IgG. Platelet-depletion resulted in a significant reduction in albuminuria (7 +/- 2 vs. 55 +/- 10 mg/24 h) and fractional albumin excretion (0.045 +/- 0.01 vs. 0.410 +/- 0.09) compared with controls. The decrease in albuminuria was not due to differences in blood or glomerular neutrophil counts, complement, renal function, or glomerular antibody binding. Platelet-depleted rats had equivalent subendothelial deposits and glomerular endothelial cell injury but had minimal platelet infiltrates and fibrin deposition compared with controls. These studies demonstrate a role for platelets in mediating acute neutrophil-induced glomerular injury and proteinuria in this model of GN.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195.
authors.forename: R J
authors.initials: RJ
authors.lastname: Johnson
authors.forename: C E
authors.initials: CE
authors.lastname: Alpers
authors.forename: P
authors.initials: P
authors.lastname: Pritzl
authors.forename: M
authors.initials: M
authors.lastname: Schulze
authors.forename: P
authors.initials: P
authors.lastname: Baker
authors.forename: C
authors.initials: C
authors.lastname: Pruchno
authors.forename: W G
authors.initials: WG
authors.lastname: Couser
mesh_terms: D000818:Animals; D001666:Binding Sites, Antibody; D001792:Blood Platelets / Q000276:immunology / Q000473:pathology*; D003165:Complement System Proteins / Q000378:metabolism; D005921:Glomerulonephritis / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D007105:Immune Complex Diseases / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D007106:Immune Sera; D007677:Kidney Function Tests; D007678:Kidney Glomerulus / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D009504:Neutrophils / Q000276:immunology / Q000473:pathology*; D011507:Proteinuria / Q000209:etiology; D051381:Rats; D011919:Rats, Inbred Strains; D013921:Thrombocytopenia / Q000209:etiology / Q000276:immunology / Q000473:pathology
chemical_list: D007106:Immune Sera; D003165:Complement System Proteins