Phospholipids in plant and animal chromatin.
Isolated hepatic nuclei and hepatic chromatin have been analysed for their DNA, RNA, protein and phospholipid content. The protein/DNA ratio is 3 for nuclei and 1.95 for chromatin extracted from Triton X-100 treated nuclei. The phospholipids, (2.36 +/- 0.91 (S.D.) per cent of the total nuclear material), are lost during the chromatin preparation mainly during the Triton X-100 washings of the nuclei. Nevertheless, 10 per cent of the total nuclear phospholipids remain bound to the chromatin. The comparative analysis of both nuclei and chromatin shows a difference in phospholipids and fatty acid composition. Thus, the chromatin-associated phospholipid cannot be attributed simply to contaminating nuclear membrane. This is supported by the autoradiographic study of semi-thin sections of interphase nuclei from root apices of Vicia faba in which [3H] ethanolamine is clearly localized in the chromatin and nucleolar regions of the nuclei.
Viola-Magni
M P
MP
Gahan
P B
PB
Pacy
J
J
D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002843:Chromatin / Q000302:isolation & purification; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D007887:Fabaceae / Q000032:analysis; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008099:Liver / Q000032:analysis; D017830:Octoxynol; D010743:Phospholipids / Q000032:analysis*; D010946:Plants, Medicinal; D011092:Polyethylene Glycols; D051381:Rats; D011919:Rats, Inbred Strains
D002843:Chromatin; D010743:Phospholipids; D011092:Polyethylene Glycols; D017830:Octoxynol; D005952:Glucose-6-Phosphatase