A new 5'-nucleotidase inhibitor, nucleoticidin. II. Physico-chemical properties and structure elucidation.
A novel 5'-nucleotidase inhibitor, named nucleoticidin, was isolated from a fermentation broth of Pseudomonas sp. The molecular weight was estimated by gel filtration to be over 1,000,000. Nucleoticidin is composed of D-glucose and D-mannose at a molar ratio of 1.7 to 1.0. Combined analyses using chemical and physico-chemical methods, such as gas liquid chromatography and mass fragmentography, revealed that nucleoticidin has a structural unit with mannosyl residues at the terminal of a (1----4) linked D-glucosyl main chain with beta-configuration.
Uchino
K
K
Ogawara
H
H
Akiyama
T
T
Fukuchi
A
A
Shibata
S
S
Takahashi
K
K
Narui
T
T
D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002241:Carbohydrates / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D004586:Electrophoresis / Q000379:methods; D005285:Fermentation; D006868:Hydrolysis; D008745:Methylation; D009708:Nucleotidases / Q000037:antagonists & inhibitors*; D011549:Pseudomonas / Q000378:metabolism; D013057:Spectrum Analysis; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D002241:Carbohydrates; D009708:Nucleotidases; C030287:nucleotidase