Iontophoresis of epinephrine isomers to rabbit eyes induced HSV-1 ocular shedding.
Iontophoresis of 0.01% levo(-) epinephrine for 8 min at 0.8 mAmp once daily for 3 consecutive days induces ocular shedding of herpes simplex virus type 1 (HSV-1) in latently infected rabbits. In the present experiment, we tested dextro(+) and levo(-) epinephrine for their comparative effects on induced HSV-1 ocular shedding. One hundred percent of the eyes shed virus after either 0.01% dextro(+) or levo(-) epinephrine iontophoresis (8 min, 0.8 mAmp). However, the shedding frequency caused by 0.005% levo(-) epinephrine was significantly higher (P less than 0.05) than that by 0.005% dextro(+) epinephrine when the iontophoresis was conducted at 0.4 mAmp for 4 min. Iontophoresis of 0.001% levo(-) epinephrine for 8 min at 0.8 mAmp once daily for 3 consecutive days and iontophoresis of 0.001% dextro(+) epinephrine for 8 min at 0.8 mAmp once daily for 3 consecutive days did not induce HSV-1 ocular shedding in latently infected rabbits. The data suggest that the mechanism of induction of HSV-1 ocular shedding by epinephrine is correlated to the receptor potency of levo(-) epinephrine.
Hill
J M
JM
Shimomura
Y
Y
Kwon
B S
BS
Gangarosa
L P
LP
D000818:Animals; D004837:Epinephrine / Q000031:analogs & derivatives / Q000494:pharmacology*; D007478:Iontophoresis; D011817:Rabbits; D018139:Simplexvirus / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
D004837:Epinephrine