[Effect of natural or synthetic detergents on the transport of D-glucose in the membranes of vesicles of the brush border of the intestine of the rabbit].
We describe here the effects of natural and synthetic detergents on the D-glucose transport into brush-border membranes of vesicles of rabbit's intestine. Two synthetic detergents: Triton X-100 and dodecyltrimethylammonium bromide have been found very strong inhibitors (more than 50 p. 100 of inhibition of maximal D-glucose uptake). Kinetic studies showed that these detergents behaved as mixed type inhibitors. The Na+-dependent transport of amino acids (aspartic acid, lysine, phenylalanine) is only poorly affected by dodecyltrimethylammonium bromide, while Triton X-100 inhibits unspecifically all the transport studied.
Favilli
F
F
Département des Sciences biochimiques, Université de Florence, Italie.
Iantomasi
T
T
Stio
M
M
Treves
C
C
Vanni
P
P
Vincenzini
M T
MT
D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology*; D005947:Glucose / Q000493:pharmacokinetics*; D007421:Intestine, Small / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D011817:Rabbits; D012964:Sodium / Q000493:pharmacokinetics; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D009004:Monosaccharide Transport Proteins; D013501:Surface-Active Agents; D012964:Sodium; D005947:Glucose
Action de détergents naturels ou synthétiques sur le transport du D-glucose dans les membranes des vésicules de la bordure en brosse de l'intestin du lapin.