Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme.
A mouse lactate dehydrogenase-A cDNA was isolated and it was shown to contain the 393bp of the protein-coding sequence and 488bp of the 3' untranslated region. The amino acid sequence deduced from its open reading frame provided independent evidence for the sequence of residues 201-331 of mouse LDH-A subunit (muscle). This cDNA clone was used as a probe to isolate a mouse genomic clone containing a truncated, processed LDH-A pseudogene. This pseudogene showed 81.6% homology at 713 positions compared with the LDH-A cDNA sequence. The divergence of this pseudogene was estimated to have occurred 39 million years ago.
Akai
K
K
Yagi
K
K
Tiano
H F
HF
Pan
Y C
YC
Shimizu
M
M
Fong
K
K
Jungmann
R A
RA
Li
S S
SS
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D005796:Genes; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D051379:Mice
D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase; D004262:DNA Restriction Enzymes