Messenger RNAs from the transforming region of bovine papilloma virus type I.
Messenger RNAs present in C127 mouse cells transformed by bovine papilloma virus type 1 (BPV-1) were studied by the S1 nuclease protection technique, Northern blotting, and electron microscopic heteroduplex analysis. The results revealed at least five classes of spliced mRNAs which we designate types 1 to 5. They had a common poly(A) addition site located at co-ordinate 53 and all mRNAs, except the type 3 mRNAs, contained an exon located between co-ordinates 41 and 53. In the type 1 mRNAs this exon was connected to a very short leader sequence located around co-ordinate 31. The type 2 mRNAs contained 220 to 400-nucleotide long leaders which were located approximately 1.5 X 10(3) base-pairs further upstream. Two different subclasses of type 2 molecules (2A and 2B) were identified and these had slightly different leaders. The type 4 mRNAs contained a bipartite leader, whereas the type 5 mRNAs carried an approximately 900-nucleotide long leader. The type 3 mRNAs consisted of a main exon located between co-ordinates 32 and 53, linked to the same leader as is present in the type 2A mRNAs. A cap site which presumably is utilized by the type 2A, type 3, type 4 and type 5 mRNAs was mapped at nucleotide 89 in the BPV-1 sequence. A putative cap site for the type 1 mRNAs was mapped at co-ordinate 31.
Stenlund
A
A
Zabielski
J
J
Ahola
H
H
Moreno-Lopez
J
J
Pettersson
U
U
D000818:Animals; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002417:Cattle; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral; D004720:Endonucleases; D005814:Genes, Viral; D051379:Mice; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D012332:RNA, Heterogeneous Nuclear / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases
D004279:DNA, Viral; D012332:RNA, Heterogeneous Nuclear; D012333:RNA, Messenger; D012367:RNA, Viral; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases