Expression of A and B types of monoamine oxidase in differentiated neuroblastoma hybrid cells.
The total activities of monoamine oxidase (MAO) and the ratio of type B/type A activities were determined in mouse neuroblastoma N1E-115 cells, and in NX31T and NG108-15 hybrid cells derived from mouse neuroblastoma X rat sympathetic ganglion hybrid or mouse neuroblastoma X rat glioma hybrid cells. N1E-115 and NX31T cells possessed type A activities exclusively, although NG108-15 cells showed both type A (65-90%) and type B (10-35%) MAO activities. The activity of type A MAO in NX31T and N1E-115 cells was relatively constant during culturing periods in the presence or absence of dibutyryl cyclic AMP (Bt2cAMP), whereas total MAO activity and the ratio of type B MAO/type A MAO in NG108-15 cells increased as a function of culture periods. Prostaglandin E1 (PGE1) and theophylline, the best known combination to increase intracellular cyclic AMP content of NG108-15 cells, caused similar increases of MAO and of the type B/type A ratio in NG108-15 cells. The results suggest that MAO activity and expression of MAO B activity are regulated in NG108-15 cells in a cyclic AMP-dependent manner.
Nakano
T
T
Nagatsu
T
T
Higashida
H
H
D000527:Alprostadil; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D006822:Hybrid Cells / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D008995:Monoamine Oxidase / Q000378:metabolism*; D009447:Neuroblastoma / Q000201:enzymology*; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats; D013806:Theophylline / Q000494:pharmacology
D007527:Isoenzymes; D011458:Prostaglandins E; D003994:Bucladesine; D013806:Theophylline; D008995:Monoamine Oxidase; D000527:Alprostadil