Uptake of a fluorinated bisphosphonate by cultured bones.
The uptake of bisphosphonates into bone was studied using 19-day-old fetal rat bones cultured with a new fluorinated bisphosphonate, difluoromethylidene bisphosphonate (F2MBP). F2MBP uptake was assessed by determining the weight percent of fluoride using electron probe microanalysis. By 30 min the weight percent of fluoride was significantly greater in the F2MBP-treated bones than in controls and continually increased throughout the duration of the experiment to reach a fluoride concentration 6-fold greater than controls after 120 h of incubation. When the peripheral cortical bone was analyzed separately from the interior trabecular bone in the F2MBP-treated bones, the fluoride concentration in the periphery increased until 24 h and then remained somewhat constant, while the interior, which is more actively remodeling, showed a continual increase. The uptake of F2MBP during the 1 to 6 h time intervals demonstrated no differences between vital and devitalized bone and, thus, is not cell-mediated. Because analysis of free fluoride in F2MBP media incubated with bones showed that the concentration of fluoride was less than 1% of the total amount of fluoride, the fluoride detected by the probe was most likely that of the intact molecule and not free fluoride. The rapid uptake of the F2MBP molecule was supported by assessing the effects of short-term F2MBP treatment on subsequent bone resorption, as determined by the release of 45Ca from prelabeled bones. Bones treated with F2MBP for only 5 min exhibited reductions in the percentage of 45Ca released during the remainder of the 120 h incubation period similar to that when F2MBP was continuously in the medium.(ABSTRACT TRUNCATED AT 250 WORDS)
Rowe
D J
DJ
Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City 52242.
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D000818:Animals; D001862:Bone Resorption / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism*; D002132:Calcium Radioisotopes / Q000378:metabolism; D004002:Clodronic Acid / Q000031:analogs & derivatives / Q000493:pharmacokinetics*; D046508:Culture Techniques; D004164:Diphosphonates / Q000493:pharmacokinetics*; D004577:Electron Probe Microanalysis; D004622:Embryo, Mammalian; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D002132:Calcium Radioisotopes; D004164:Diphosphonates; D004002:Clodronic Acid; C041121:difluoromethylene diphosphonate