Movement initiation characteristics in young adult rats in relation to the high- and low-affinity agonist states of the striatal D2 dopamine receptor.
Changes in the speed of movement initiation as a function of age, brain damage, or rat strain are associated with altered characteristics of nigrostriatal dopamine (DA) neurons and of striatal D2 DA receptors. In the present study we investigated the relationship between movement initiation (response parameters: percent of successful responses and response latency) and the agonist binding states of the D2 DA receptor in corpus striatum in 3-month-old Sprague-Dawley rats (n = 51). In contrast to the typical experimental procedure, the variances of the behavioral and receptor binding data were intentionally made as small as possible to provide the most stringent test of putative relationships among variables. Rats were trained to release a lever as rapidly as possible in response to a light/buzzer (CS) combination in order to avoid a mild footshock (UCS). Percent avoidance scores, latencies of the fastest successful trials (successful latencies) and mean latencies for all responses (mean latencies) were collected for 1000-, 500-, 300- and 200-ms CS-UCS intervals. Twenty-four hours following the last behavioral test, animals were euthanized for measurements of the high- and low-affinity binding of DA to D2 receptors in corpus striatum. The standard errors of the mean for both behavioral and receptor binding parameters were, generally, less than 10%. The tightness of the receptor binding data appeared to be related to a lack of biological variance in the animals rather than to an artifact associated with the behavioral testing procedure, since a parallel experiment indicated that different numbers of behavioral shaping sessions had no effects on striatal D2 binding characteristics.(ABSTRACT TRUNCATED AT 250 WORDS)
Wilcox
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RE
Department of Pharmacology and Toxicology, College of Pharmacy, University of Texas, Austin 78712.
Mudie
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Mayfield
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Young
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RK
Spirduso
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WW
D000818:Animals; D001362:Avoidance Learning; D001667:Binding, Competitive; D003342:Corpus Striatum / Q000502:physiology*; D004597:Electroshock; D007700:Kinetics; D008297:Male; D008433:Mathematics; D008959:Models, Neurological; D009043:Motor Activity; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000378:metabolism*; D017448:Receptors, Dopamine D2; D013134:Spiperone / Q000378:metabolism
D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D013134:Spiperone