title: Bovine interleukin 2: production by an E-rosette-defined lymphocyte subpopulation.
abstract: E-rosette-separated bovine peripheral blood lymphocyte subpopulations were examined for ability to produce interleukin 2 (IL 2). Sequential E-rosetting techniques resulted in three T-cell subpopulations and a non-T population. Separated cells were stimulated with Con A and the resulting culture supernatants were assayed for IL 2 activity on IL 2-dependent cells. The bovine T-cell subpopulation which rosetted with both neuraminidase-treated and 2-aminoethylisothiouronium bromide (AET)-treated erythrocytes was found to produce significantly more IL 2 than the other T-cell subpopulations or the non-T population. These results suggest that this population may have a T-helper cell function. IL 2-dependent cells were found to be predominately T-cells by E-rosetting, were lymphoblastoid in appearance and surface immunoglobulin negative. Conditioned media containing IL 2 were used to demonstrate cytotoxic T-cell activity against allogeneic lymphocytes in peripheral blood lymphocytes.
authors.affiliation: Department of Molecular Biology, College of Agriculture, University of Wyoming, Laramie 82071.
authors.forename: P T
authors.initials: PT
authors.lastname: Zelarney
authors.forename: E L
authors.initials: EL
authors.lastname: Belden
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D007376:Interleukin-2 / Q000096:biosynthesis*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000145:classification / Q000276:immunology*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D007376:Interleukin-2; D003208:Concanavalin A