title: Regulation of the Ig kappa-chain enhancer by the adenovirus E1A gene products. Repression in lymphoid cells, activation in fibroblasts.
abstract: Proteins encoded by early region 1A (E1A) regulate transcription of viral and cellular genes. The mechanism of this trans-activation is not understood, but is of considerable interest as an example of transcription regulation through a cellular intermediate. We have therefore studied the effect of E1A products on the activity of the kappa L chain gene promoter and enhancer. By using transient and stable transfections into lymphoid and nonlymphoid cells, we found that the E1A proteins have a pleiotropic effect on the regulation of the mouse kappa-chain gene enhancer. In lymphoid cells the E1A products repress kappa-chain enhancer, whereas in fibroblasts, the kappa-chain enhancer is activated by the E1A products whether the E1A gene is in an extrachromosomal location or stably integrated in the genome. Furthermore, a functional kappa-chain promoter, containing the octanucleotide and "TATA" sequences is needed in order to be transcribed in E1A-producing cells. This ability of E1A products to negatively and positively regulate kappa-chain transcription may reflect a more general phenomenon in which a given cellular protein could participate in a variety of different cellular controls.
authors.affiliation: Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Bergman
authors.forename: D
authors.initials: D
authors.lastname: Shavit
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000502:physiology; D017456:Adenovirus Early Proteins; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005347:Fibroblasts / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins; D014158:Transcription, Genetic
chemical_list: D017456:Adenovirus Early Proteins; D007145:Immunoglobulin kappa-Chains; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins