title: Photo-oxidation of tetracycline adsorbed on hydroxyapatite in relation to the light-induced staining of teeth.
abstract: In an attempt to understand the chemistry of the light-induced staining of children's teeth by tetracycline, we studied the photo-chemical behavior of tetracycline adsorbed on hydroxyapatite, as a simple model of enamel. Tetracycline was strongly bound by hydroxyapatite to give a pale yellow material which, under ultraviolet light, showed a bright yellow fluorescence (lambda max 525 nm). On exposure of this material to the radiation from a medium-pressure mercury lamp, the fluorescence gradually disappeared, and a red-purple product was formed. Photo-acoustic spectroscopy was employed to follow the disappearance of tetracycline and the concomitant formation of the red-purple product, the spectrum of which (lambda max 530 nm) led to the conclusion that it was 4 alpha, 12 alpha-anhydro-4-oxo-4-dedimethylaminotetracycline (AODTC). This assignment was supported by the observation that 1 mole of oxygen was absorbed per mole of adsorbed tetracycline converted to the red-purple product. It is suggested that the formation of AODTC on hydroxyapatite probably takes place by a mechanism of photo-oxidation similar to that already proposed for solutions of tetracycline, and that the formation of AODTC in children's teeth is responsible for the light-induced staining caused by tetracycline.
authors.forename: A K
authors.initials: AK
authors.lastname: Davies
authors.forename: R B
authors.initials: RB
authors.lastname: Cundall
authors.forename: Y
authors.initials: Y
authors.lastname: Dandiker
authors.forename: M A
authors.initials: MA
authors.lastname: Slifkin
mesh_terms: D002648:Child; D003116:Color; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites; D008027:Light / Q000009:adverse effects*; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010777:Photochemistry; D013752:Tetracycline / Q000009:adverse effects* / Q000528:radiation effects; D014075:Tooth Discoloration / Q000139:chemically induced*
chemical_list: D006882:Hydroxyapatites; D017886:Durapatite; D013752:Tetracycline