title: Increase in DNA polymerase alpha in nuclei of chicken kidney cells after avian adenovirus infection.
abstract: DNA polymerase activity which sedimented at about 7s in the presence of 0.5M KC1 increased in cultured kidney cells after infection with a chicken embryo lethal orphan virus, an avian adenovirus. Elevated levels of antigenic material to a monoclonal antibody specific for the large subunit of chicken DNA polymerase alpha were also detected in nuclei of virus-infected cells by an immunofluorescence method. Since the elevations of DNA polymerase activity and of nuclear immunofluorescence was not inhibited with 1-beta-D-arabinofuranosylcytosine, the early viral gene(s) seems to be responsible for the induction of the synthesis and nuclear accumulation of DNA polymerase alpha.
authors.forename: A
authors.initials: A
authors.lastname: Matsukage
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Yasue
authors.forename: M
authors.initials: M
authors.lastname: Ishibashi
mesh_terms: D000257:Adenoviridae Infections / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D000259:Aviadenovirus; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens; D004257:DNA Polymerase II / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D004257:DNA Polymerase II