Establishment of a statistical base for use of ELISA in diagnostic serology for infectious bovine rhinotracheitis.
The critical statistical parameters of an enzyme-linked immunosorbent assay were determined to enable quantitation of antibody responses in cattle affected with infectious bovine rhinotracheitis. A system of controlling well-to-well variations in optical density reading across a microtitre plate was evolved and dose--response assays were carried out to determine the dilution of serum which gave the greatest discrimination between acute and convalescent sera from an infected animal. Use of a standard serum was studied in further assays. An increase in optical density value of 0.15 was set as a diagnostic criterion for a significantly rising antibody response. This compared well with the conventional criterion of a fourfold rise in virus neutralizing antibody titre.
Hebert
C N
CN
Edwards
S
S
Bushnell
S
S
Jones
P C
PC
Perry
C T
CT
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D007242:Herpesvirus 1, Bovine / Q000276:immunology; D007124:Immunoenzyme Techniques; D007241:Infectious Bovine Rhinotracheitis / Q000175:diagnosis*; D009500:Neutralization Tests; D012015:Reference Standards; D012698:Serologic Tests; D013223:Statistics as Topic
D000914:Antibodies, Viral