title: Monoclonal antibody-ricin conjugate cytotoxic to cells expressing the common acute lymphoblastic leukemia antigen (CALLA).
abstract: The monoclonal antibody PHM-6, which is specific for the common acute lymphoblastic leukemia antigen (CALLA), was conjugated to the plant toxin ricin. Binding of the PHM-6-ricin conjugate to cells via the ricin molecule was blocked by the presence of 100 mM lactose. The IC50 (concentration resulting in 50% inhibition) of the PHM-6-ricin conjugate for the CALLA-positive KM-3 cell line was 280-fold greater than for bone marrow stem cells, indicating the potential of this conjugate for immunological purging of autologous remission marrow.
authors.affiliation: Haematology Department, Royal Perth Hospital.
authors.forename: J M
authors.initials: JM
authors.lastname: Pelham
authors.forename: B F
authors.initials: BF
authors.lastname: Meyer
authors.forename: R P
authors.initials: RP
authors.lastname: Herrmann
authors.forename: R E
authors.initials: RE
authors.lastname: Davis
authors.forename: C L
authors.initials: CL
authors.lastname: Raphael
authors.forename: N
authors.initials: N
authors.lastname: Kraft
authors.forename: R C
authors.initials: RC
authors.lastname: Atkins
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D001853:Bone Marrow / Q000187:drug effects; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007201:Indicator Dilution Techniques; D007942:Leukemia, Experimental / Q000473:pathology; D015260:Neprilysin; D012276:Ricin / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D013234:Stem Cells / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D012276:Ricin; D015260:Neprilysin