title: The immediate-early enhancer element of herpes simplex virus type 1 can replace a regulatory region of the c-Ha-ras1 oncogene required for transformation.
abstract: A 0.8-kilobase SacI DNA fragment in the distal 5'-noncoding region of the c-Ha-ras1 oncogene hybridized to high guanine X cytosine sites of herpes simplex virus type 1 (HSV-1) DNA restriction fragments. Nucleotide sequence comparisons localized one of these sites to the intergenic region of HSV between the immediate-early genes coding for IEmRNA-3 and IEmRNA-4/5 that has enhancer-type activity. We tested the possibility that the HSV-1 enhancer and the upstream c-Ha-ras1 SacI fragment were functionally related by assaying for the capacity of recombinant plasmids in which the HSV-1 enhancer replaced the oncogene 0.8-kilobase SacI fragment to transform NIH/3T3 cells. Deletion of the 0.8-kilobase SacI fragment abolished the biological activity of c-Ha-ras1, but its replacement by the HSV-1 enhancer fully restored it. These results confirm the enhancer properties of the HSV-1 immediate-early intergenic region and suggest that c-Ha-ras1 sequences contained within the 0.8-kilobase SacI fragment plays a role in the transcriptional activation of the oncogene.
authors.forename: A
authors.initials: A
authors.lastname: Puga
authors.forename: J
authors.initials: J
authors.lastname: Gomez-Marquez
authors.forename: P R
authors.initials: PR
authors.lastname: Brayton
authors.forename: E M
authors.initials: EM
authors.lastname: Cantin
authors.forename: L K
authors.initials: LK
authors.lastname: Long
authors.forename: M
authors.initials: M
authors.lastname: Barbacid
authors.forename: A L
authors.initials: AL
authors.lastname: Notkins
mesh_terms: D001483:Base Sequence; D002472:Cell Transformation, Viral; D005786:Gene Expression Regulation; D009857:Oncogenes; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics*