Interactions of arachidonate metabolism and protein kinase C in mediating neutrophil function.
Diglyceride activators of protein kinase C (i.e., 1-0-myristoyl-, 1-0-palmitoyl-, and 1-0-oleoyl-2-acetylglycerol) interacted synergistically with an arachidonate metabolite, 5-hydroxyicosatetraenoate, to stimulate neutrophil degranulation and superoxide anion generation. Contrastingly, combinations of 15-hydroxyicosatetraenoate with the glycerides or 5-hydroxyicosatetraenoate with a dialkylglyceride (1-0-hexadecyl-2-ethylglycerol) produced no such synergy. The data support a view of stimulus-response coupling wherein protein kinase C is activated in parallel with the mobilization of arachidonate. Respective products of these events, e.g., phosphorylated proteins and hydroxyicosatetraenates, then interact to mediate function.
O'Flaherty
J T
JT
Schmitt
J D
JD
Wykle
R L
RL
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D003594:Cytoplasmic Granules / Q000502:physiology; D004075:Diglycerides / Q000494:pharmacology; D004357:Drug Synergism; D004789:Enzyme Activation / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D011493:Protein Kinase C; D011494:Protein Kinases / Q000097:blood*; D013481:Superoxides / Q000378:metabolism
D001095:Arachidonic Acids; D004075:Diglycerides; D006893:Hydroxyeicosatetraenoic Acids; D013481:Superoxides; D016718:Arachidonic Acid; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; C038237:1-oleoyl-2-acetylglycerol; C042096:1-O-hexadecanoyl-2-acetylglycerol; C044777:1-O-myristoyl-2-acetyl-glycerol; D011494:Protein Kinases; D011493:Protein Kinase C; D009113:Muramidase; D005966:Glucuronidase