Induction of cAMP-dependent protein kinase I during human monocyte differentiation.
Differentiation of human peripheral blood monocytes into macrophages was accompanied by induction of the regulatory subunit of cAMP-dependent protein kinase I as determined by photoaffinity labeling of cytosol proteins with 8-N3-[32P]cAMP and DEAE-Sephacel chromatography. The appearance of cAMP-dependent protein kinase I in macrophages was not due to translocation from the particulate fraction of monocytes. The regulatory subunit of cAMP-dependent protein kinase II was present in both monocytes and in vitro-differentiated macrophages. Protein kinase I in macrophages demonstrated higher affinity for 8-N3-cAMP (KD = 0.7 nM) than did protein kinase II from either monocytes (KD = 14.5 nM) or macrophages (KD = 4.9 nM). These studies demonstrate induction of the regulatory subunit of cAMP-dependent protein kinase I during the differentiation of a normal human cell and support the hypothesis that cAMP may regulate some stages of differentiation.
Wenger
G D
GD
O'Dorisio
M S
MS
D001386:Azides / Q000378:metabolism; D001667:Binding, Competitive; D002454:Cell Differentiation; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology / Q000201:enzymology*; D011494:Protein Kinases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis; D011983:Receptors, Purinergic; D013347:Subcellular Fractions / Q000201:enzymology
D001386:Azides; D007527:Isoenzymes; D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; C017042:8-azidoadenosine-3',5'-monophosphate; D000242:Cyclic AMP; D011494:Protein Kinases