title: Removal of a 67-base-pair sequence in the noncoding region of protooncogene fos converts it to a transforming gene.
abstract: Transformation of fibroblasts by protooncogene fos (c-fos) requires the linkage of viral long terminal repeat (LTR) sequences and interruption of 3'-noncoding sequences. We have identified an A + T-rich stretch of 67 nucleotides, located 627-693 base pairs downstream from the coding domain and 123-189 base pairs upstream from the putative poly(A) addition site, removal of which confers transforming activity to the c-fos gene. A novel regulation of the expression of the c-fos gene is proposed, which may be functional in vivo to prevent the gene from becoming an oncogene.
authors.forename: F
authors.initials: F
authors.lastname: Meijlink
authors.forename: T
authors.initials: T
authors.lastname: Curran
authors.forename: A D
authors.initials: AD
authors.lastname: Miller
authors.forename: I M
authors.initials: IM
authors.lastname: Verma
mesh_terms: D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D002872:Chromosome Deletion; D005786:Gene Expression Regulation; D051379:Mice; D009154:Mutation; D009690:Nucleic Acid Conformation; D009857:Oncogenes; D011061:Poly A / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D009053:Sarcoma Viruses, Murine / Q000235:genetics*
chemical_list: D011061:Poly A