An antiserum to the C-terminus of the Alzheimer amyloid precursor recognizes a soluble 70 kDa protein.
A rabbit antiserum to the C-terminus of the putative brain amyloid precursor was used to probe Western blots of tissue proteins separated by SDS-PAGE. The antiserum specifically labelled a protein of approx. 70 kDa in the Tris buffer-soluble fraction of brain samples from rat, Alzheimer subjects, cases of young and old Down's syndrome, and age-matched controls. The 70 kDa protein was present in low concentrations in human liver and kidney, and was undetectable in human skeletal muscle. The 70 kDa protein may be a metabolite of the amyloid precursor.
Simpson
J
J
Department of Pharmacology, University of Edinburgh, Scotland.
Bladon
C M
CM
Yates
C M
CM
Harmar
A J
AJ
D000328:Adult; D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D000682:Amyloid / Q000032:analysis* / Q000276:immunology; D016564:Amyloid beta-Protein Precursor; D001923:Brain Chemistry; D004314:Down Syndrome / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera; D007223:Infant; D008875:Middle Aged; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis; D009928:Organ Specificity; D011498:Protein Precursors / Q000032:analysis* / Q000276:immunology
D000682:Amyloid; D016564:Amyloid beta-Protein Precursor; D007106:Immune Sera; D009419:Nerve Tissue Proteins; D011498:Protein Precursors