Monoclonal antibody-ricin conjugate cytotoxic to cells expressing the common acute lymphoblastic leukemia antigen (CALLA).
The monoclonal antibody PHM-6, which is specific for the common acute lymphoblastic leukemia antigen (CALLA), was conjugated to the plant toxin ricin. Binding of the PHM-6-ricin conjugate to cells via the ricin molecule was blocked by the presence of 100 mM lactose. The IC50 (concentration resulting in 50% inhibition) of the PHM-6-ricin conjugate for the CALLA-positive KM-3 cell line was 280-fold greater than for bone marrow stem cells, indicating the potential of this conjugate for immunological purging of autologous remission marrow.
Pelham
J M
JM
Haematology Department, Royal Perth Hospital.
Meyer
B F
BF
Herrmann
R P
RP
Davis
R E
RE
Raphael
C L
CL
Kraft
N
N
Atkins
R C
RC
D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D001853:Bone Marrow / Q000187:drug effects; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007201:Indicator Dilution Techniques; D007942:Leukemia, Experimental / Q000473:pathology; D015260:Neprilysin; D012276:Ricin / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D013234:Stem Cells / Q000187:drug effects
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D012276:Ricin; D015260:Neprilysin