title: Biochemical characteristics of the Mr 52,000 component of Akazara scallop troponin.
abstract: Some biochemical properties of the Mr 52,000 component of Akazara scallop striated adductor troponin, which had been tentatively identified as troponin-I, were compared with those of rabbit troponin-I. Both the Mr 52,000 component and rabbit troponin-I together with rabbit tropomyosin inhibited the Mg-ATPase activity of rabbit reconstituted actomyosin to 1/10 of the original activity. The inhibition was neutralized by the addition of Akazara scallop and rabbit troponin-C or Patinopecten scallop calmodulin. The Mr 52,000 component and rabbit troponin-I were insoluble below 0.15 M KCl, but were solubilized by complexing with an equimolar amount of troponin-C or calmodulin. On alkaline urea-polyacrylamide gel electrophoresis, the Mr 52,000 component as well as rabbit troponin-I was found to form a stable complex with troponin-C or calmodulin in the presence of Ca2+.
authors.affiliation: Department of Chemistry, Faculty of Fisheries, Hokkaido University.
authors.forename: T
authors.initials: T
authors.lastname: Ojima
authors.forename: K
authors.initials: K
authors.lastname: Nishita
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000032:analysis / Q000037:antagonists & inhibitors; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D008974:Mollusca / Q000032:analysis*; D009218:Myosins / Q000037:antagonists & inhibitors; D012995:Solubility; D014336:Troponin / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D019209:Troponin C
chemical_list: D002135:Calcium-Binding Proteins; D002147:Calmodulin; D014336:Troponin; D019209:Troponin C; D017301:Ca(2+) Mg(2+)-ATPase; D009218:Myosins