title: Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs.
abstract: Two distinct but distantly related complementary DNAs encoding proteins sharing human interleukin-1 (IL-1) activity (termed IL-1 alpha and IL-1 beta), were isolated from a macrophage cDNA library. The primary translation products of the genes are 271 and 269 amino acids long, although expression in Escherichia coli of the carboxy-terminal 159 and 153 amino acids produces IL-1 biological activity.
authors.forename: C J
authors.initials: CJ
authors.lastname: March
authors.forename: B
authors.initials: B
authors.lastname: Mosley
authors.forename: A
authors.initials: A
authors.lastname: Larsen
authors.forename: D P
authors.initials: DP
authors.lastname: Cerretti
authors.forename: G
authors.initials: G
authors.lastname: Braedt
authors.forename: V
authors.initials: V
authors.lastname: Price
authors.forename: S
authors.initials: S
authors.lastname: Gillis
authors.forename: C S
authors.initials: CS
authors.lastname: Henney
authors.forename: S R
authors.initials: SR
authors.lastname: Kronheim
authors.forename: K
authors.initials: K
authors.lastname: Grabstein
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000302:isolation & purification; D014981:Xenopus
chemical_list: D004270:DNA, Circular; D007375:Interleukin-1; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes