Combined macroscopic and microscopic detection of viral genes in tissues.
A hybridization technique has been devised for detecting and quantitating viral genes in tissues that combines macroscopic and microscopic analyses in the same section. The method is based on dual labeling virus-specific probes with 125I and 35S to generate signals that can be detected both with X-ray films and nuclear track emulsions. The regions of increased hybridization evident in the X-ray film serve as a guide to the portion of the section that warrants microscopic examination. Detection of viral RNA in tissues with visna virus and viral DNA with hepatitis B virus are illustrated, and potential applications of this technique in virology and other disciplines are discussed.
Haase
A T
AT
Gantz
D
D
Blum
H
H
Stowring
L
L
Ventura
P
P
Geballe
A
A
Moyer
B
B
Brahic
M
M
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000382:microbiology; D006528:Carcinoma, Hepatocellular / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics; D007457:Iodine Radioisotopes; D008099:Liver / Q000382:microbiology; D008113:Liver Neoplasms / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D012756:Sheep; D013462:Sulfur Radioisotopes; D014790:Visna-maedi virus / Q000235:genetics
D004279:DNA, Viral; D007457:Iodine Radioisotopes; D012367:RNA, Viral; D013462:Sulfur Radioisotopes