Murine c-fms cDNA: cloning, sequence analysis and retroviral expression.
We have isolated and sequenced a cDNA clone coding for the murine c-fms gene. The 3677 nucleotide cDNA clone codes for a protein of 976 amino acids, which has 76% and 75% homology to the v-fms and human proteins, respectively. The predicted membrane protein, c-fms, has an amino terminal signal sequence and external, transmembrane and cytoplasmic domains. The homology between murine c-fms and v-fms or human c-fms is the strongest (90%-95%) in the cytoplasmic domain and weakest (59%-63%) in the external domain. The c-fms clone was inserted into a retroviral vector containing a neomycin resistance gene and cell lines resistant to G418 were isolated. These cell lines expressed protein which was immunoprecipitated by anti-c-fms antibodies and of the same size as murine c-fms. These cells were also found to specifically bind human CSF-1.
Rothwell
V M
VM
Cell Biology Department, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Rohrschneider
L R
LR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D051379:Mice; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016186:Receptor, Macrophage Colony-Stimulating Factor; D012190:Retroviridae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D011518:Proto-Oncogene Proteins; D004247:DNA; D011505:Protein-Tyrosine Kinases; D016186:Receptor, Macrophage Colony-Stimulating Factor