Alkali-labile structures linked to the 5' ends of Bacillus subtilis short DNA chains.
Alkali-labile portion covalently linked to the 5' ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature sensitive DNA polymerase I mutant, which accumulates nascent DNA fragments at a restrictive temperature. A novel oligonucleotide structure as well as mono- to triribonucleotide stretches were isolated at the 5' end of the short DNA chains. A structure for the novel oligonucleotide is proposed to be p5' X3' pp5' rN, where X represents unidentified nucleoside with a peculiar property. Possible metabolic relationship between these molecules and primer RNA has been discussed.
Yasuda
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H
Okazaki
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D000469:Alkaline Phosphatase; D001412:Bacillus subtilis / Q000235:genetics*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D009841:Oligonucleotides / Q000032:analysis; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D010761:Phosphorus Radioisotopes; D012265:Ribonucleotides / Q000032:analysis
D004269:DNA, Bacterial; D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D012265:Ribonucleotides; D004259:DNA-Directed DNA Polymerase; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I