Induction of tumor suppression and delayed-type footpad reaction by transfer of lymphocytes sensitized to a xenogenized tumor variant.
Splenocytes immune to a highly immunogenic ("xenogenized", L5178Y/DTIC) variant of a murine lymphoma exert anti-parental-tumor activity in a systemic adoptive transfer system, the effect being apparently associated with the Lyt-2- fraction of the lymphocyte population. During investigation of the mechanisms of this protection, we found that the L5178Y/DTIC tumor-immune lymphocytes exhibited an appreciable anti-L5178Y delayed-type hypersensitivity (DTH) response. Enrichment of those lymphocytes in L3T4+ cells significantly enhanced the protective effect as well as the DTH reaction, whereas the use of an anti-L3T4 but not anti-Lyt-2 reagent blocked both activities. In vitro, lymphocyte proliferation against L5178Y cells occurred and was apparently associated with the Lyt-2- fraction of a population of L5178Y/DTIC immune splenocytes.
Bianchi
R
R
Institute of Pharmacology, University of Perugia, Italy.
Romani
L
L
Puccetti
P
P
Fioretti
M C
MC
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007116:Immunization, Passive; D007940:Leukemia L5178 / Q000276:immunology; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology