title: Calcium uptake and inositol trisphosphate-induced calcium release from parathyroid gland membranes.
abstract: The manner in which changes in Ca2+ are translated by the parathyroid gland into a signal for secretion of its major secretory proteins, parathormone and secretory protein-I, is not understood. In order to gain insight into this mechanism, Ca2+ transport was evaluated in porcine parathyroid gland membranes prepared by homogenization and differential centrifugation. The membranes were considered to be intact and appropriately 'sided' since they contained secretory protein-I and exhibited ATP-dependent uptake of [45Ca2+]. About 1 mol of ATP was hydrolyzed per mol of Ca2+ transported, signifying that the ATPase activity was relevant to the Ca2+ transport system of the membranes. Inositol trisphosphate, which is believed to be a universal signal for generating a change in cytosolic Ca2+ by discharging Ca2+ from internal membranes, caused the parathyroid membranes to release accumulated Ca2+. The membranes were able to partially reaccumulate the discharged Ca2+. The data suggest that intracellular Ca2+ metabolism in the parathyroid cell is similar to that of other secretory cells. If a change in intracellular concentration of this ion is involved in secretion, it may occur locally within the cytosol.
authors.affiliation: Department of Biochemistry, University of Louisville, KY 40292.
authors.forename: W L
authors.initials: WL
authors.lastname: Dean
authors.forename: S E
authors.initials: SE
authors.lastname: Adunyah
authors.forename: D V
authors.initials: DV
authors.lastname: Cohn
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000494:pharmacology*; D008566:Membranes / Q000378:metabolism; D010280:Parathyroid Glands / Q000187:drug effects / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000378:metabolism; D013403:Sugar Phosphates / Q000494:pharmacology*; D013552:Swine
chemical_list: D007295:Inositol Phosphates; D010281:Parathyroid Hormone; D013403:Sugar Phosphates; D015544:Inositol 1,4,5-Trisphosphate; D000252:Calcium-Transporting ATPases; D002118:Calcium