A mouse tRNA pseudogene derived from a tRNA(Trp) coding sequence.
The identification and characterization of a mouse tRNA(Trp) pseudogene is reported. A synthetic oligonucleotide (31 mer), identical with the 3' half of a tRNA(Trp), was used to examine three mouse lambda clones that are known to contain clusters of tRNA genes. A fragment of one of these lambda clones strongly hybridizes to the oligonucleotide; the sequence of this region shows the presence of a gene significantly homologous to the chick tRNA(Trp). However two point mutations and a three base deletion prevent the folding of a possible transcript of this gene. The presence of a conserved promoter sequence for RNA polymerase III, that should allow the transcription of this gene, does not ensure the transcription of the gene, at least in our in vitro system.
Ammendola
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R
Istituto di Scienze Biochimiche, II Facoltà di Medicina e Chirurgia, Napoli.
Russo
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T
Cimino
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F
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D003062:Codon; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D010761:Phosphorus Radioisotopes; D011544:Pseudogenes; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012364:RNA, Transfer, Trp / Q000235:genetics*; D014158:Transcription, Genetic
D003062:Codon; D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D012345:RNA, Transfer, Amino Acid-Specific; D012364:RNA, Transfer, Trp